Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3.
Functional analysis of hyperthermophilic endocellulase from Pyrococcus horikoshii by crystallographic snapshots.
Structure of hyperthermophilic endocellulase from Pyrococcus horikoshii.
The role of disulfide bond in hyperthermophilic endocellulase.
Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins.
Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.
Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA.
Crystal structure and mutational study of a unique SpoU family archaeal methylase that forms 2'-O-methylcytidine at position 56 of tRNA.
Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA.
Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Structure of an archaeal homologue of the bacterial Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase.
Crystal structures of tyrosyl-tRNA synthetases from Archaea.
A structural code for discriminating between transcription signals revealed by the feast/famine regulatory protein DM1 in complex with ligands.
A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3.
Structural basis for the transglycosylase activity of a GH57-type glycogen branching enzyme from Pyrococcus horikoshii.
Cloning, purification, crystallization and preliminary crystallographic analysis of acylphosphatase from Pyrococcus horikoshii OT3.
Crystal structure of a hyperthermophilic archaeal acylphosphatase from Pyrococcus horikoshii -- structural insights into enzymatic catalysis, thermostability, and dimerization.
Crystallization and preliminary crystallographic analysis of an acylphosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii.
Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes.
Structure of mannosyl-3-phosphoglycerate phosphatase from Pyrococcus horikoshii.
Molecular structure and novel DNA binding sites located in loops of flap endonuclease-1 from Pyrococcus horikoshii.
Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis.
Molecular basis for the subunit assembly of the primase from an archaeon Pyrococcus horikoshii.
Structure of a putative 2'-5' RNA ligase from Pyrococcus horikoshii.
The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94 A resolution reveals a possible open form with a wider active-site cleft.
Crystal structure of aspartate racemase from Pyrococcus horikoshii OT3 and its implications for molecular mechanism of PLP-independent racemization.
Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases.
Structure of aspartate racemase complexed with a dual substrate analogue, citric acid, and implications for the reaction mechanism.
Structure of a haloacid dehalogenase superfamily phosphatase PH1421 from Pyrococcus horikoshii OT3: oligomeric state and thermoadaptation mechanism.
Crystal structure of the putative RNA methyltransferase PH1948 from Pyrococcus horikoshii, in complex with the copurified S-adenosyl-L-homocysteine.
Structural basis for translation factor recruitment to the eukaryotic/archaeal ribosomes.
Three binding sites for stalk protein dimers are generally present in ribosomes from archaeal organism.
Crystal structure of a novel FAD-, FMN-, and ATP-containing L-proline dehydrogenase complex from Pyrococcus horikoshii.
Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii.
Temperature dependence of the enzyme-substrate recognition mechanism.
The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii.
Crystal Structures of a Hyperthermophilic Archaeal Homoserine Dehydrogenase Suggest a Novel Cofactor Binding Mode for Oxidoreductases.
A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii: substrate selection achieved through multimerization.
A family of metal-dependent phosphatases implicated in metabolite damage-control.
The crystal structure of a novel SAM-dependent methyltransferase PH1915 from Pyrococcus horikoshii.
Crystal structure of the PH1932 protein, a unique archaeal ArsR type winged-HTH transcription factor from Pyrococcus horikoshii OT3.
Crystal structure of PH1733, an aspartate racemase homologue, from Pyrococcus horikoshii OT3.
Structure of protein PH0536 from Pyrococcus horikoshii at 1.7 A resolution reveals a novel assembly of an oligonucleotide/oligosaccharide-binding fold and an alpha-helical bundle.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii.
Crystal structure of hypothetical protein PH0828 from Pyrococcus horikoshii.
Observation of a calcium-binding site in the gamma-class carbonic anhydrase from Pyrococcus horikoshii.
A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3.
Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.
Thermodynamic analysis of a multifunctional RNA-binding protein, PhoRpp38, in the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
Computational design of enone-binding proteins with catalytic activity for the Morita-Baylis-Hillman reaction.
Crystal structure of the probable haloacid dehalogenase PH0459 from Pyrococcus horikoshii OT3.
Design and evolution of an enzyme with a non-canonical organocatalytic mechanism.
Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.
An unexpected species determined by X-ray crystallography that may represent an intermediate in the reaction catalyzed by quinolinate synthase.
Crystal structure of the NAD biosynthetic enzyme quinolinate synthase.
Crystal structures of the iron-sulfur cluster-dependent quinolinate synthase in complex with dihydroxyacetone phosphate, iminoaspartate analogues, and quinolinate.
Structure of quinolinate synthase from Pyrococcus horikoshii in the presence of its product, quinolinic acid.
Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme.
Crystal structure of hypothetical protein PH0642 from Pyrococcus horikoshii at 1.6A resolution.
The structures of the C146A variant of the amidase from Pyrococcus horikoshii bound to glutaramide and acetamide suggest the basis of amide recognition.
The impact of CRISPR repeat sequence on structures of a Cas6 protein-RNA complex.
Investigating a catalytic mechanism of hyperthermophilic L-threonine dehydrogenase from Pyrococcus horikoshii.
Kinetic study of thermostable L-threonine dehydrogenase from an archaeon Pyrococcus horikoshii.
L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3: gene cloning and enzymatic characterization.
The first crystal structure of L-threonine dehydrogenase.
A tRNA splicing operon: Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation.
Molecular basis of alanine discrimination in editing site.
The structure of alanyl-tRNA synthetase with editing domain.
Structure of the type I L-asparaginase from the hyperthermophilic archaeon Pyrococcus horikoshii at 2.16 angstroms resolution.
Purification and characterization of the first archaeal glutamate decarboxylase from Pyrococcus horikoshii.
A hyperthermostable D-ribose-5-phosphate isomerase from Pyrococcus horikoshii characterization and three-dimensional structure.
Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.
Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2.
Crystal structure of archaeal toxin-antitoxin RelE-RelB complex with implications for toxin activity and antitoxin effects.
Crystal structure of a core domain of stomatin from Pyrococcus horikoshii illustrates a novel trimeric and coiled-coil fold.
Crystal structure of a membrane stomatin-specific protease in complex with a substrate peptide.
Structural and biochemical analysis of a thermostable membrane-bound stomatin-specific protease.
Acta Crystallogr. F Struct. Biol. Commun.
Structure of the archaeal chemotaxis protein CheY in a domain-swapped dimeric conformation.
Feast/famine regulation by transcription factor FL11 for the survival of the hyperthermophilic archaeon Pyrococcus OT3.
Interactions between the archaeal transcription repressor FL11 and its coregulators lysine and arginine.
The archaeal feast/famine regulatory protein: potential roles of its assembly forms for regulating transcription.
Crystal structure of hyperthermophilic archaeal initiation factor 5A: a homologue of eukaryotic initiation factor 5A (eIF-5A).
Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution.
Structural and functional analysis of the archaeal endonuclease Nob1.
Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.
The three-dimensional structure of septum site-determining protein MinD from Pyrococcus horikoshii OT3 in complex with Mg-ADP.
Structure based hyperthermostability of archaeal histone HPhA from Pyrococcus horikoshii.
Reconstitution of diphthine synthase activity in vitro.
Structures of two archaeal diphthine synthases: insights into the post-translational modification of elongation factor 2.
Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii.
Crystal structure of product-bound complex of UDP-N-acetyl-d-mannosamine dehydrogenase from Pyrococcus horikoshii OT3.
Purification, crystallization and preliminary X-ray diffraction studies of a putative UDP-N-acetyl-D-mannosamine dehydrogenase from Pyrococcus horikoshii OT3.
An archaeal Dim2-like protein, aDim2p, forms a ternary complex with a/eIF2 alpha and the 3' end fragment of 16S rRNA.
Crystal structure of Dim2p: a preribosomal RNA processing factor, from Pyrococcus horikoshii OT3 at 2.30 A.
Crystal structure of a ribonuclease P protein Ph1601p from Pyrococcus horikoshii OT3: an archaeal homologue of human nuclear ribonuclease P protein Rpp21.
Reconstitution of archaeal ribonuclease P from RNA and four protein components.
Structure of an archaeal homolog of the human protein complex Rpp21-Rpp29 that is a key core component for the assembly of active ribonuclease P.
Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation.
Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate.
Crystal structure of alanyl-tRNA synthetase editing-domain homolog (PH0574) from a hyperthermophile, Pyrococcus horikoshii OT3 at 1.45 A resolution.
First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.
Bifunctional isocitrate-homoisocitrate dehydrogenase: a missing link in the evolution of beta-decarboxylating dehydrogenase.
Alkyl hydroperoxide reductase dependent on thioredoxin-like protein from Pyrococcus horikoshii.
Oxidative stress response in an anaerobic hyperthermophilic archaeon: presence of a functional peroxiredoxin in Pyrococcus horikoshii.
Structure of peroxiredoxin from the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii.
Structure of PIN-domain protein PH0500 from Pyrococcus horikoshii.
Common evolutionary origin of swapped-hairpin and double-psi beta barrels.
Crystal structure of a novel type of ornithine delta-aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii.
The first archaeal agmatinase from anaerobic hyperthermophilic archaeon Pyrococcus horikoshii: cloning, expression, and characterization.
Crystal structure of SAM-dependent methyltransferase from Pyrococcus horikoshii.
Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site.
Accurate design of co-assembling multi-component protein nanomaterials.
Geometric Lessons and Design Strategies for Nanoscale Protein Cages.
Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system.
X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii.
A novel thermostable membrane protease forming an operon with a stomatin homolog from the hyperthermophilic archaebacterium Pyrococcus horikoshii.
Molecular structure of a novel membrane protease specific for a stomatin homolog from the hyperthermophilic archaeon Pyrococcus horikoshii.
Novel dimer structure of a membrane-bound protease with a catalytic Ser-Lys dyad and its linkage to stomatin.
Acta Crystallogr Sect F Struct Biol Cryst Commun
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3.
Binding thermodynamics of a glutamate transporter homolog.
Coupled ion binding and structural transitions along the transport cycle of glutamate transporters.
Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter.
Crystal structure of an asymmetric trimer of a bacterial glutamate transporter homolog.
Direct visualization of glutamate transporter elevator mechanism by high-speed AFM.
Functional characterization of a Na+-dependent aspartate transporter from Pyrococcus horikoshii.
Structure of a glutamate transporter homologue from Pyrococcus horikoshii.
The uncoupled chloride conductance of a bacterial glutamate transporter homolog.
Transport mechanism of a bacterial homologue of glutamate transporters.
Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62).
S-adenosyl-L-methionine:hydroxide adenosyltransferase: a SAM enzyme.
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies.
Structural basis of CoA recognition by the Pyrococcus single-domain CoA-binding proteins.
Structure determination of a novel protein by sulfur SAD using chromium radiation in combination with a new crystal-mounting method.
Structures of dimeric nonstandard nucleotide triphosphate pyrophosphatase from Pyrococcus horikoshii OT3: functional significance of interprotomer conformational changes.
Structural analysis of the transcriptional regulator homolog protein from Pyrococcus horikoshii OT3.
Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis.
Structural insights into the mechanism of archaellar rotational switching.
Crystal structure of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii.