Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
Acyl-ester intermediate; for transpeptidase activity
Penicillin-binding protein transpeptidase
Proton donor; for transglycosylase activity
Transglycosylase PBP1b N-terminal transmembrane
4Fe-4S ferredoxin-type domain-containing protein
Pimeloyl-[acyl-carrier protein] methyl ester esterase
Probable HTH-type transcriptional regulator SinR
ABC-type glycine betaine transport system substrate-binding
Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic
Low molecular weight protein-tyrosine-phosphatase Wzb
Surface presentation of antigens protein SpaO
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Pyruvate dehydrogenase E1 component middle
Cytochrome oxidase subunit II copper A binding
Cytochrome oxidase subunit II transmembrane region profile
Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
Increases ATPase-inducing activity and histidine transport; when associated with K-47.
Slight decrease in ATPase-inducing activity and histidine transport.
Strong decrease in ATPase-inducing activity and histidine transport.
Oxygen-dependent coproporphyrinogen-III oxidase
Periplasmic nitrate reductase, electron transfer subunit
Prokaryotic-type class I peptide chain release factors
4-hydroxyphenylacetate 3-monooxygenase reductase component
Ribosomal RNA large subunit methyltransferase G
Anaerobic glycerol-3-phosphate dehydrogenase subunit B
3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase
23S rRNA (uracil(747)-C(5))-methyltransferase RlmC
Orn/Lys/Arg decarboxylases family 1 pyridoxal-P attachment site
Probable HTH-type transcriptional regulator LeuO
Unable to grow in minimal glucose medium; requires thiamine for growth. Is defective in FAD binding.
Transposase for insertion sequence element IS200
Lipopolysaccharide biosynthesis protein RfbH
Abrogates host cell invasion and effector secretion; when associated with A-76.
Abrogates host cell invasion and effector secretion; when associated with A-79.
Abrogates host cell invasion and effector secretion; when associated with A-8. Can secrete effector proteins; when associated with A-20.
Can only secrete early substrates such as InvJ/ScpT, PrgJ/SctI and PrgI/SctF. Can polymerize into filaments in vitro and in vivo, but the stability of the filaments is compromised.
Can only secrete early substrates such as InvJ/ScpT, PrgJ/SctI and PrgI/SctF. Can polymerize into filaments in vitro, but not in vivo. Cannot enter cultured epithelial cells.
Can only secrete early substrates such as InvJ/ScpT, PrgJ/SctI and PrgI/SctF. Does not yield assembled filaments in vitro. Cannot enter cultured epithelial cells.
Can secrete effector proteins; when associated with A-5. Exhibits constitutive secretion of substrates. Retains the ability to display SipD/SctA at the tip of the needle filament.
Can secrete only early substrates. Can assemble needle filaments in vitro, but shows a filament assembly defect in vivo. Can invade cultured epithelial cells.
Cannot secrete the translocase SipB/SctE and SptP. Retains the ability to assemble needle complexes, but is not able to invade cultured epithelial cells.
Cannot secrete the translocases SipB/SctE and SipC/SctB. Retains the ability to assemble needle complexes, but is not able to invade cultured epithelial cells.
Cannot secrete the translocases SipB/SctE and SipC/SctB. Shows increased secretion of SptP. Retains the ability to assemble needle complexes, but is not able to invade cultured epithelial cells.
Decreases invasiveness. Abrogates host cell invasion and effector secretion; when associated with A-5.
Exhibits constitutive secretion of substrates. Can invade cultured epithelial cells.
Exhibits constitutive secretion of substrates. Does not display SipD/SctA at the tip of the needle filament. Is non-invasive. Can polymerize into filaments in vitro.
Exhibits constitutive secretion of substrates. Retains the ability to display SipD/SctA at the tip of the needle filament.
Exhibits constitutive secretion of substrates. Retains the ability to display SipD/SctA at the tip of the needle filament. Does not yield assembled filaments in vitro.
SPI-1 type 3 secretion system needle filament protein
Shows a specific deficiency in the secretion of the translocase SipB/SctE. Retains the ability to assemble needle complexes, but is not able to invade cultured epithelial cells.
Shows a specific deficiency in the secretion of the translocase SipB/SctE. Shows fully assembled needle complexes, but has a reduced number of needle filaments. Is able to invade cultured epithelial cells.
Shows a specific deficiency in the secretion of the translocase SipB/SctE. Shows fully assembled needle complexes, but has a reduced number of needle filaments. Is drastically affected in its ability to enter into host cells.
Shows a specific deficiency in the secretion of the translocase SipB/SctE. Shows fully assembled needle complexes. Is able to invade cultured epithelial cells.
Transcription termination/antitermination protein NusG
Lipoprotein-releasing system ATP-binding protein LolD
Threonine/serine exporter-like N-terminal
Pyruvate phosphate dikinase AMP/ATP-binding
Major facilitator superfamily (MFS) profile
Putative colanic acid biosynthesis glycosyltransferase WcaL
Protoporphyrinogen IX dehydrogenase [quinone]
Molybdenum import ATP-binding protein ModC
Guanine nucleotide exchange factor sopE2
Thiol:disulfide interchange protein DsbC
DNA-binding and helicase activity, interacts with RecC
Nuclease activity, interacts with RecD and RecA
Extradiol ring-cleavage dioxygenase class III enzyme subunit B
Oxygen-insensitive NAD(P)H nitroreductase
Essential for the oxygen-regulated activity
Fumarate and nitrate reduction regulatory protein
Sulfate transport system permease protein CysT
In gyrA209TS; a temperature-sensitive mutant, 15% decreased growth rate, decreased (-) supercoiling even at permissive temperature.
In gyrA213TS; a temperature-sensitive mutant, decreased (-) supercoiling even at permissive temperature.
O-(5'-phospho-DNA)-tyrosine intermediate
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Ribosomal RNA large subunit methyltransferase I
Regulator of nucleoside diphosphate kinase N-terminal
Transcription elongation factor GreA/GreB C-terminal
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
O-(3'-phospho-DNA)-tyrosine intermediate
Electron transfer flavoprotein alpha/beta-subunit N-terminal
Surface presentation of antigens protein SpaM
sn-glycerol-3-phosphate transport system permease protein UgpE
FMN-dependent NADH:quinone oxidoreductase
PTS system glucose-specific EIIA component
Tele-phosphohistidine intermediate; for EIIA activity
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like
Mechanosensitive ion channel inner membrane
Cyclic pyranopterin monophosphate synthase
Fe-S hydro-lyase tartrate dehydratase beta-type catalytic
Anaerobic ribonucleoside-triphosphate reductase
High frequency lysogenization protein HflD homolog
Mandelate racemase/muconate lactonizing enzyme C-terminal
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Adenosylcobinamide-GDP ribazoletransferase
Acetylornithine/succinyldiaminopimelate aminotransferase
3'(2'),5'-bisphosphate nucleotidase CysQ
Loss of activity. Cannot bind acetyl-CoA. Does not interact with pro-PanD.
DNA-3-methyladenine glycosylase AlkA N-terminal
DNA polymerase III subunit delta C-terminal
Sugar fermentation stimulation protein A
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Resolvase/invertase-type recombinase catalytic
Acetaldehyde dehydrogenase (acetylating) EutE
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Phosphocysteine intermediate; for EIIB activity
In isoform Soluble copper chaperone CopA(Z).
p-hydroxybenzoic acid efflux pump subunit AaeB
Sec-independent protein translocase protein TatB
Putative surface-exposed virulence protein BigA
Probable cobalt-factor III C(17)-methyltransferase
Chromosomal replication initiator protein DnaA
Oligopeptide transport ATP-binding protein OppF
Cysteine sulfenic acid (-SOH) intermediate
Spermidine/putrescine transport system permease protein PotB
Peptide transport system ATP-binding protein SapD
Ribosomal RNA small subunit methyltransferase B
Involved in Mg(2+) ion dislocation from EF-Tu
Aminodeoxychorismate synthase component 1
N6-(4-deoxychorismate)-lysine intermediate
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE
Peptidase M1 alanyl aminopeptidase C-terminal
Peptidase M1 alanyl aminopeptidase Ig-like fold
Peptidase M1 membrane alanine aminopeptidase
Complements deletion as well as wild-type.
Slight reduction in complementation of deletion.
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
Glucose-6-phosphate isomerase prokaryote
Uncharacterized N-acetyltransferase YjgM
Yop protein translocation protein D periplasmic
Putative D-alanyl-D-alanine carboxypeptidase
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
Ionotropic glutamate receptor C-terminal
Solute-binding protein family 3/N-terminal
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
5,10-methylenetetrahydrofolate reductase
Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom
Increases geranylation activity. Lack of geranylation activity; when associated with A-155.
Reduces the level of geranylation but does not abolish it.
Type III restriction-modification enzyme StyLTI Res subunit
Cytochrome c-type biogenesis protein CcmE
Helical; Signal-anchor for type II membrane protein
2-fold decrease in enzymatic activity, but no effect on substrate affinity.
20-fold decrease in enzymatic activity, but no effect on substrate affinity.
47-fold decrease in enzymatic activity, but no effect on substrate affinity.
7-fold decrease in enzymatic activity, but no effect on substrate affinity.
More than 5-fold decrease in substrate affinity.
Nearly no decrease in enzymatic activity.
C-terminal domain (CTD), required but not sufficient to bind 70S or 30S ribosomes
Large ribosomal subunit assembly factor BipA
No longer associates with 70S ribosomes.
No longer binds to 70S ribosomes in presence of GMPPNP, altered secondary structure.
No longer binds to 70S ribosomes in presence of GMPPNP.
No longer binds to 70S ribosomes, has no GTPase activity.
Still binds to 70S ribosomes in presence of GMPPNP.
Membrane-bound lytic murein transglycosylase F
Secretion system apparatus lipoprotein SsaJ
tRNA N6-adenosine threonylcarbamoyltransferase
TonB-dependent receptor-like beta-barrel
Lipopolysaccharide 1,3-galactosyltransferase
Negative modulator of initiation of replication
High-affinity branched-chain amino acid transport system permease protein LivM
Surface presentation of antigens protein SpaP
1000-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
2-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
750-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
Inactive. Retains the same oligomeric state of the wild-type.
Periplasmic binding protein/LacI sugar binding
Aspartate carbamoyltransferase catalytic subunit
Low level streptomycin and spectinomycin resistance.
Ribosomal RNA small subunit methyltransferase G
Glutathione transport system permease protein GsiC
Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK
Participates in proton transfer during catalysis
D-alanyl-D-alanine carboxypeptidase DacD
Phosphoadenosine phosphosulphate reductase
Bacterial microcompartment shell protein EutS
EutC and EutE constructs are no longer targeted to EutS or eutSMNLKBMCs. BMCs are formed in E.coli by both sets of genes.
EutC constructs are targeted to EutSBMCs, cells are somewhat elongated.
No longer targets EutC-tagged constructs to BMCs, probably forms flat symmetric hexamers.
Greasy slide, important in sugar transport
Bifunctional polymyxin resistance protein ArnA
Proton acceptor; for decarboxylase activity
Proton donor; for decarboxylase activity
Proton donor; for formyltransferase activity
In gyrB1820TS; a temperature-sensitive mutant, 50% decreased growth rate, greatly decreased (-) supercoiling even at permissive temperature.
In gyrB652TS; a temperature-sensitive mutant, no growth at 42 degrees Celsius, 36% decreased growth rate at 30 degrees Celsius (permissive), low k(cat) at 30, 37 and 42 degrees Celsius, in vivo significantly decreased (-) supercoiling even at permissive temperature.
Probable csgAB operon transcriptional regulatory protein
L-methionine sulfoximine/L-methionine sulfone acetyltransferase
High-affinity zinc uptake system protein ZnuA
Adenosylcobalamin/alpha-ribazole phosphatase
Cytochrome b561 bacterial/Ni-hydrogenase
Putative 2-aminoethylphosphonate transport system permease protein PhnV
Membrane-bound lytic murein transglycosylase C
DNA-directed DNA polymerase family B exonuclease
DNA-directed DNA polymerase family B multifunctional
Spermidine/putrescine-binding periplasmic protein
Ion-translocating oxidoreductase complex subunit A
Iron-sulfur cluster insertion protein ErpA
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Loss of kinase activity. No effect on translocation of the protein.
Cobalt-precorrin-2 C(20)-methyltransferase
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Outer membrane protein assembly factor BamA
Small ribosomal subunit biogenesis GTPase RsgA
Dual-specificity RNA pseudouridine synthase RluF
10-fold reduced MIC for streptomycin, no change in spectinomycin resistance, reduced streptomycin but not spectinomycin binding.
2.7-fold reduced MIC for streptomycin, loss of spectinomycin resistance, no change in ATP or antibiotic binding.
4-5-fold reduced MIC for streptomycin and spectinomycin, very little spectinomycin binding.
5-fold reduced MIC for streptomycin, 1.5-fold reduced MIC for spectinomycin resistance, reduced streptomycin but not spectinomycin binding.
8-fold reduced MIC for streptomycin, loss of spectinomycin resistance.
Aminoglycoside (3"") (9) adenylyltransferase
Loss of streptomycin and spectinomycin resistance, 4-fold decreased ATP-binding, significantly decreased streptomycin binding, no spectinomycin binding.
Loss of streptomycin and spectinomycin resistance, no ATP-binding, near wild-type streptomycin binding, significantly decreased spectinomycin binding.
Loss of streptomycin and spectinomycin resistance, no ATP-binding, very little antibiotic binding.
Propanediol dehydratase-reactivating factor small subunit
Putative membrane-bound redox modulator Alx
Thiamine transport system permease protein ThiP
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
DNA-directed RNA polymerase subunit alpha
Important for beta-aspartyl-AMP intermediate formation
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
Undecaprenyl-phosphate galactose phosphotransferase
O-(phosphoribosyl dephospho-coenzyme A)serine
Transcriptional regulator SgrR N-terminal HTH
Leucyl/phenylalanyl-tRNA--protein transferase
Galactose/methyl galactoside import ATP-binding protein MglA
3,4-dihydroxy-2-butanone 4-phosphate synthase
BREX system Lon protease-like BrxL N-terminal
RNA polymerase-binding transcription factor DksA
Allows movement of 1,2-PD and NAD(+)/NADH across the BMC, excretes slightly more propionaldehyde, makes normal BMCs.
Bacterial microcompartment shell protein PduT
No transport of 1,2-PD or NAD(+)/NADH across the BMC, makes normal BMCs.
No transport of 1,2-PD or NAD(+)/NADH across the BMC, no change in propionaldehyde excretion, makes normal BMCs.
Purified protein is more stable, crystallized, has a triangular pore of about 46 Angstroms(2) area. Allows movement of 1,2-PD and NAD(+)/NADH across the BMC, excretes slightly more propionaldehyde, makes normal BMCs.
Probable L-ascorbate-6-phosphate lactonase UlaG
Negative regulator of flagellin synthesis
Reversibly protonated during proton transport
Magnesium and cobalt efflux protein CorC
Acetolactate synthase isozyme 3 small subunit
Succinate dehydrogenase cytochrome b556 subunit
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Ribosomal RNA small subunit methyltransferase E PUA-like
Ribosomal RNA small subunit methyltransferase E methyltransferase
Glucose-1-phosphate cytidylyltransferase
Surface presentation of antigens protein SpaS
Thiosulfate reductase electron transfer subunit PhsB
Localizes almost exclusively to the cytosol of transfected cells.
Ion-translocating oxidoreductase complex subunit D
Sensory histidine kinase/phosphatase NtrB
Maltose/maltodextrin transport system permease protein MalF
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
Low affinity potassium transport system protein Kup
Lipopolysaccharide core biosynthesis protein RfaZ
2-dehydro-3-deoxyphosphooctonate aldolase
Outer membrane protein assembly factor BamD
TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase
Crossover junction endodeoxyribonuclease RuvC
D-galactarate/Altronate dehydratase C-terminal
Histidine/lysine/arginine/ornithine transport system permease protein HisQ
Nucleoside-specific channel-forming protein Tsx
Does not complement deletion, 0.6% activity in vitro. No metal cofactor.
Does not complement deletion, 1.2% activity in vitro. No metal cofactor.
Does not complement deletion, 20% activity in vitro. Reduces metal content of enzyme, complements a deletion.
No effect in vivo or in vitro. No binding of Co(2+).
Reduces metal content of enzyme, does not complement deletion. No binding of Co(2+).
Potassium-transporting ATPase KdpC subunit
Type I restriction enzyme StySJI methylase subunit
Quercetin 2,3-dioxygenase C-terminal cupin
Ribosomal RNA small subunit methyltransferase C
Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
Considerably reduced DNA-binding, in fragment 91-137.
Loss of DNA-binding, in fragment 91-137.
No change in DNA-binding, in fragment 91-137.
No effect on DNA-binding or physical properties.
No longer binds Hha or YdgT, slightly altered DNA-binding, may self associate into longer than wild-type filaments. Derepression.
No longer binds Hha or YdgT, slightly altered DNA-binding, wild-type self-association. Derepression of some H-NS-regulated genes, acts similarly to an hha deletion strain.
No longer binds Hha, still able to bind YdgT.
Reduced DNA-binding, in fragment 91-137.
Dual-specificity RNA methyltransferase RlmN
Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal
Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
Pyridoxine/pyridoxamine 5'-phosphate oxidase
Cysteine sulfinic acid (-SO2H); alternate
Probable protein adenylyltransferase Fic
Enzyme no longer associated with BMCs, no effect on enzyme activity.
Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal
RNA binding; important for wobble base 34 recognition
Loss of activity at elevated temperature.
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Ion-translocating oxidoreductase complex subunit B
tRNA1(Val) (adenine(37)-N6)-methyltransferase
Glycine betaine/proline betaine transport system permease protein ProW
Phosphoribosylaminoimidazole-succinocarboxamide synthase
ATP-dependent dethiobiotin synthetase BioD 2
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB
Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport
Mediates proton transfer from the protein to the inner aqueous phase
Pros-phosphohistidine intermediate; for HPr activity
Much less protein associates with BMCs, no effect on enzyme activity.
Propanal dehydrogenase (CoA-propanoylating)
Substantially decreased protein levels in BMCs, no change in enzyme activity.
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
Methylated-DNA--protein-cysteine methyltransferase
Lipopolysaccharide 1,2-glucosyltransferase
Charge relay system; for autoendoproteolytic cleavage activity
Cleavage (non-hydrolytic); by autocatalysis
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
Peptide methionine sulfoxide reductase MsrB
Holliday junction branch migration complex subunit RuvA
UDP-3-O-acyl-N-acetylglucosamine deacetylase
Glutathione-regulated potassium-efflux system protein KefC
FAD-dependent oxidoreductase 2 FAD binding
Ribosomal RNA large subunit methyltransferase F
Aerobic C4-dicarboxylate transport protein
Thiamine import ATP-binding protein ThiQ
Large ribosomal RNA subunit accumulation protein YceD
Aerobactin siderophore biosynthesis IucA/IucC-like C-terminal
Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
Maltose/maltodextrin import ATP-binding protein MalK
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
6-phosphogluconate dehydrogenase, decarboxylating
Curli production assembly/transport component CsgE
Involved in proton transfer during cleavage
Carboxy-S-adenosyl-L-methionine synthase
Phosphoenolpyruvate-dependent phosphotransferase system
Galactose/methyl galactoside import permease protein MglC
L-Threonine dehydratase biosynthetic IlvA
Polyribonucleotide nucleotidyltransferase
Glucans biosynthesis glucosyltransferase H
Acetolactate synthase isozyme 3 large subunit
Integral membrane protein YccS N-terminal
Type 1 fimbrin D-mannose specific adhesin
Interaction with polymerase core subunit RpoC
5-fold decrease in deacylase activity. Does not affect translocation by T3SS and cellular localization.
Fatty acid oxidation complex subunit alpha
Anaerobic ribonucleoside-triphosphate reductase-activating protein
Decrease in activity. Defect in histidine binding.
Intracellular growth attenuator protein IgaA
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
Galactose-1-phosphate uridylyltransferase
Abolishes the dehydrogenase and ferrochelatase activities and reduces 10-fold the methyltransferase activity.
Abolishes the dehydrogenase and ferrochelatase activities and reduces 6-fold the methyltransferase activity.
Abolishes the methyltransferase activity and increases 3 and 4-fold the dehydrogenase and ferrochelatase activities, respectively.
Abolishes the methyltransferase activity and reduces 10 and 5-fold the dehydrogenase and ferrochelatase activities, respectively.
Abolishes the methyltransferase, dehydrogenase and ferrochelatase activities.
Precorrin-2 dehydrogenase /sirohydrochlorin ferrochelatase
Uroporphyrinogen-III C-methyltransferase
Putative transcriptional regulator of 2-aminoethylphosphonate degradation operons
Glycosyltransferase subfamily 4-like N-terminal
Endo-type membrane-bound lytic murein transglycosylase A
Ion-translocating oxidoreductase complex subunit G
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
Membrane fusion protein biotin-lipoyl like
Formate hydrogenlyase transcriptional activator
Flagellar transcriptional regulator FlhD
Could provide a fine-tuning to achieve optimal pKa matching between active site and substrate
Sodium/calcium exchanger membrane region
NIF system FeS cluster assembly NifU N-terminal
Alters interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-130.
Fails to induce host endosomal tubulation; when associated with A-197.
Fails to induce host endosomal tubulation; when associated with A-201.
Loss of interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-131.
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase
Glutathionylspermidine synthase pre-ATP-grasp-like
Concentrative nucleoside transporter C-terminal
Concentrative nucleoside transporter N-terminal
SPI-1 type 3 secretion system stator protein
Tryptophan synthase beta chain-like PALP
ATP-dependent Clp protease ATP-binding subunit ClpX
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
Ribosomal large subunit pseudouridine synthase C
Alpha-glycerophosphate oxidase C-terminal
Impaired helical structure and abolished catalytic activity.
Hinge residue important for conformational flexibility
No acetyl-coenzyme A synthetase activity.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
3-keto-L-gulonate-6-phosphate decarboxylase UlaD
Basal-body rod modification protein FlgD
Maltose/maltodextrin transport system permease protein MalG
Cytochrome c-type biogenesis protein CcmF C-terminal
Biosynthetic peptidoglycan transglycosylase
Pyridoxine/pyridoxal/pyridoxamine kinase
DNA-directed RNA polymerase subunit beta'
Decreases global transcription rate, 28% decreased growth rate, increased negative supercoiling level.
Pyruvate dehydrogenase complex repressor
Multiple antibiotic resistance protein MarR
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Uncharacterized MFS-type transporter YcaD
Histidine biosynthesis bifunctional protein HisIE
Probable Sec-independent protein translocase protein TatE
Soluble pyridine nucleotide transhydrogenase
Penicillin-binding protein activator LpoB
Purine nucleoside phosphorylase DeoD-type
Surface presentation of antigens protein SpaQ
Shows thiamine-independent growth under anaerobic conditions, but thiamine dependent growth under aerobic conditions.
Influences the redox potential of the prosthetic heme and FAD groups
Involved in heme-bound ligand stabilization and O-O bond activation
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
Protein-arginine N-acetylglucosaminyltransferase SseK2
3-isopropylmalate dehydratase small subunit 2
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Medium/long-chain acyl-CoA thioesterase YigI
Complete loss of NMN adenylyltransferase activity.
Nicotinamide mononucleotide adenylyltransferase
No NMN adenylyltransferase activity. Binds DNA independently of NAD.
No NMN adenylyltransferase activity. Binds DNA independently of NAD; when associated with H-123.
No NMN adenylyltransferase activity. Binds DNA independently of NAD; when associated with N-173.
Trifunctional NAD biosynthesis/regulator protein NadR
Choloylglycine hydrolase/NAAA C-terminal
Type VI secretion system IcmF C-terminal
Lipopolysaccharide export system protein LptA
Organic solvent tolerance-like N-terminal
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
YbaK/aminoacyl-tRNA synthetase-associated
Vitamin B12 import ATP-binding protein BtuD
UTP--glucose-1-phosphate uridylyltransferase
TtsA-like Glycoside hydrolase family 108
Aspartate carbamoyltransferase regulatory chain
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Gp5/Type VI secretion system Vgr protein OB-fold
Cell division topological specificity factor
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM
Succinate--CoA ligase [ADP-forming] subunit beta
Complements a cobB deletion, produces only the long isoform. Has deacylation and phosphoribosyltransferase activities.
Complements a cobB deletion, produces only the short isoform. Has deacylation and phosphoribosyltransferase activities.
Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Putative 2-aminoethylphosphonate-binding periplasmic protein
Phosphoenolpyruvate-protein phosphotransferase
Trk system potassium uptake protein TrkH
Phosphoglycerate transport regulatory protein PgtC
Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate
Expression at concentrations above 30 uM retains a protein-protein interaction and PhoQ-independent induction of PAG. Yields insoluble purified protein above 7 uM.
Virulence transcriptional regulatory protein PhoP
No localization with flagellar basal body, thus no binding to FliM.
Suppresses a yhjH disruption mutant upon overexpression, binds FliM but no longer affects motility in this assay.
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
TRAP C4-dicarboxylate transport system permease DctM subunit
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
In strain: LT2 / SGSC1412 and S4194; resistance to polymyxin B and neutrophil proteins.
Malonyl CoA-acyl carrier protein transacylase
In strain: RKS2985, RKS2993, RKS2995 and RKS3057.
In strain: RKS3013, RKS3014 and RKS3015.
SPI-2 type 3 secretion system translocon protein SctB
ATP-dependent Clp protease proteolytic subunit
3-isopropylmalate dehydratase small subunit 1
sn-glycerol-3-phosphate import ATP-binding protein UgpC
Bacterial microcompartment shell protein EutL
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
RND efflux pump membrane fusion protein barrel-sandwich
DNA-binding transcriptional regulator NtrC
O antigen biosynthesis rhamnosyltransferase RfbN
Moves into active site upon enzyme activation, plays a role in electron transfer
Peptide transport system permease protein SapC
Ribosomal RNA small subunit methyltransferase H
Putative membrane protein insertion efficiency factor
Oxaloacetate decarboxylase gamma chain 2
SPI-2 type 3 secretion system stator protein
Alters specificity to use phenolic compounds as substrate; when associated with M-88 and M-175.
Alters specificity to use phenolic compounds as substrate; when associated with Y-80 and M-175.
Alters specificity to use phenolic compounds as substrate; when associated with Y-80 and M-88.
Decreases specific activity for DMB and adenine about 20-fold. Decreases specific activity 1600- to 15000-fold; when associated with A-317.
Decreases specific activity for DMB and adenine about 50-fold; crystals bind substrate less well. Decreases specific activity 1600- to 15000-fold; when associated with A-174.
Important for substrate positioning, might be proton acceptor
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Ribosomal RNA small subunit methyltransferase F
ABC-type transport auxiliary lipoprotein component
Succinate dehydrogenase iron-sulfur subunit
Isocitrate dehydrogenase kinase/phosphatase
Glucose-1-phosphate thymidylyltransferase
Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.
Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
DNA-directed RNA polymerase subunit beta
Can bind and transfer an Fe-S cluster, but has no detectable ATPase activity.
Can hydrolyze ATP and transfer an Fe-S cluster.
Hydrolyzes ATP and can bind an Fe-S cluster, but cluster transfer is compromised.
3-deoxy-D-manno-octulosonic-acid transferase N-terminal
Non-specific ribonucleoside hydrolase RihC
Mannose-1-phosphate guanylyltransferase ManC
Sec-independent protein translocase protein TatA
Required for dimer formation and molybdate binding
Pantothenate precursors transporter PanS
1-deoxy-D-xylulose 5-phosphate reductoisomerase
E3 ubiquitin-protein ligase catalytic domain
Cytoplasmic dimerization domain (CDD), when dimerized forms osmosensitive core
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
Ribonucleoside-diphosphate reductase 1 subunit alpha
Cytochrome C biogenesis protein transmembrane
Thiol:disulfide interchange protein DsbD N-terminal
For 3-hydroxyacyl-CoA dehydrogenase activity
Almost no change in feedback control by tryptophan.
Complete loss of feedback control by tryptophan.
Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.
Decrease in feedback control by tryptophan.
Important for interaction with partner proteins
Thiol:disulfide interchange protein DsbE
Important in orienting the L-aspartate substrate
Lipopolysaccharide core heptose(II) kinase RfaY
Methionine import ATP-binding protein MetN 2
Protein-methionine-sulfoxide reductase catalytic subunit MsrP
Putative N-acetylmannosamine-6-phosphate 2-epimerase 1
Ribosomal RNA large subunit methyltransferase H
3-methyl-2-oxobutanoate hydroxymethyltransferase
Aromatic amino acid transport protein AroP
Glycine betaine/proline betaine transport system ATP-binding protein ProV
SUF system FeS cluster assembly SufBD N-terminal
Propionate catabolism operon regulatory protein
Glutamate-1-semialdehyde 2,1-aminomutase
Glyceraldehyde-3-phosphate dehydrogenase
2-iminobutanoate/2-iminopropanoate deaminase
Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Important for catalytic activity at high pH
Xanthine-guanine phosphoribosyltransferase
May be important for providing the necessary conformational flexibility for enzymatic catalysis
Type III restriction-modification enzyme StyLTI Mod subunit
Curli production assembly/transport component CsgF
Outer-membrane lipoprotein carrier protein
Lipopolysaccharide core heptose(I) kinase RfaP
3-isopropylmalate dehydratase large subunit 2
NAD-dependent dihydropyrimidine dehydrogenase subunit PreA
Macrolide export ATP-binding/permease protein MacB
Peptide transport system ATP-binding protein SapF
Virulence genes transcriptional activator
Ethanolamine ammonia-lyase reactivase EutA
Loss of covalent pyridoxal phosphate binding.
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase
High-affinity branched-chain amino acid transport ATP-binding protein LivG
Peptidase S11 D-Ala-D-Ala carboxypeptidase A C-terminal
serine-type D-Ala-D-Ala carboxypeptidase
Localizes throughout the cytosol and not to late endocytic compartments; when associated with P-37.
Localizes throughout the cytosol and not to late endocytic compartments; when associated with R-44.
Required to target late endocytic compartments
Alters cation selectivity. Decreases H(+)- and Na(+)-coupled activity, with little effect on Li(+)-coupled melibiose transport.
Alters cation selectivity. Inhibits H(+)- and Na(+)-coupled activity, with little effect on Li(+)-coupled melibiose transport.
Alters cation selectivity. Loss of transporter activity.
Alters cation selectivity. Retains a low level of H(+)-coupled melibiose transport, but retains only a weak activity with Na(+) or Li(+).
Alters cation selectivity. Retains only a low level of H(+)-coupled melibiose binding and active transport, but Na(+) or Li(+) does not stimulate either binding or transport.
Inhibits Na(+)- and Li(+)-coupled activity, with little effect on H(+)-coupled melibiose transport.
Retains weak activity with Na(+), Li(+) or H(+).
Deoxyuridine 5'-triphosphate nucleotidohydrolase
DnaJ homologue subfamily C member 28 conserved
Holliday junction branch migration complex subunit RuvB
Phosphatidic acid phosphatase type 2/haloperoxidase
Recombination-promoting nuclease pSLT051
(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase
DNA-directed RNA polymerase subunit omega
Confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
In strain: JB279; fast-growing suppressor of streptomycin resistant S12 mutant K42N.
Important for substrate binding and specificity
SPI-2 type 3 secretion system translocon protein SctE
Stationary-phase-induced ribosome-associated protein
Propanediol dehydratase-reactivating factor large subunit
ATP-dependent dethiobiotin synthetase BioD 1
RNA 2-O ribose methyltransferase substrate binding
Considerably decreased amount of PduL in BMCs, enzyme has greater than wild-type activity.
Decreased amount of PduL in BMCs, enzyme has wild-type activity.
Shikimate dehydrogenase substrate binding N-terminal
Molybdopterin-synthase adenylyltransferase
Glycine betaine/proline betaine-binding periplasmic protein
HTH-type transcriptional regulatory protein TyrR
Glucose-6-phosphate dehydrogenase C-terminal
Glucose-6-phosphate dehydrogenase NAD-binding
Ribosomal large subunit pseudouridine synthase D
Endolytic peptidoglycan transglycosylase RlpA
Ornithine carbamoyltransferase, catabolic
Type-F conjugative transfer system protein TraW N-terminal
Confers streptomycin independence on the streptomycin-dependent mutant L-90.
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
Erythromycin biosynthesis protein CIII-like central
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Ascorbate-specific PTS system EIIA component
Release factor glutamine methyltransferase
Ion-translocating oxidoreductase complex subunit E
Appears to be important in orienting the phosphate for catalysis
Essential for prephenate dehydratase activity
Interacts with tRNA; defines subfamily-specific binding signature
Peptide transport system permease protein SapB
Elongation factor P--(R)-beta-lysine ligase
Lack of ATPase activity. Cannot dissociate SseB/SsaE complex.
Retains ATPase activity, but does not interact with the chaperone SrcA. Strong virulence attenuation phenotype.
Glutathione-regulated potassium-efflux system ancillary protein KefG
Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)
Acetyl-CoA hydrolase/transferase C-terminal
Acetyl-CoA hydrolase/transferase N-terminal
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Inhibitor of hydrogen peroxide resistance
Large-conductance mechanosensitive channel
Imidazole glycerol phosphate synthase subunit HisF
Nitric oxide reductase FlRd-NAD(+) reductase
Sulfite reductase [NADPH] flavoprotein alpha-component
Required for zinc-mediated homotetramerization and catalytic activity
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
Oligopeptide transport system permease protein OppC
Aminodeoxychorismate synthase component 2
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Histidine/lysine/arginine/ornithine transport system permease protein HisM
Abolished protein-arginine N-acetylglucosaminyltransferase activity.
N-beta-linked (GlcNAc) arginine; by autocatalysis
Protein-arginine N-acetylglucosaminyltransferase SseK1
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Type I restriction enzyme StySJI specificity subunit
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Positions MEP for the nucleophilic attack
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
PduOC is no longer red, the mutation in the whole protein does not complement as well as wild-type.
Formate-dependent phosphoribosylglycinamide formyltransferase
Autoinducer 2 import system permease protein LsrD
Putative glucose-6-phosphate 1-epimerase
Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase C-terminal
Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase N-terminal
Ribonucleoside-diphosphate reductase 2 subunit beta
Adaptive response protein AidB N-terminal
Ribosomal protein uS12 methylthiotransferase RimO
Arylamine N-acetyltransferase / N-hydroxyarylamine O-acetyltransferase
Loss of O- and N-acetyltransferase activities.
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
Maltose/maltodextrin-binding periplasmic protein
Glutathione-regulated potassium-efflux system ancillary protein KefF
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Osmoprotectant import permease protein OsmW
Peptidyl-lysine N-acetyltransferase YjaB
Nicotinate-nucleotide adenylyltransferase
Ribosomal RNA large subunit methyltransferase M
Reduced enzymatic activity and poor motility.
Reduced enzymatic activity and poor motility; strongly reduced enzymatic activity and severely attenuated motility; when associated with N-248.
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
Dual-specificity RNA pseudouridine synthase RluA
Transcription antitermination protein NusB
7 X 2 AA approximate tandem repeats of E-P
7 X 2 AA approximate tandem repeats of K-P
Histidine biosynthesis bifunctional protein HisB
Outer membrane protein assembly factor BamB
HTH-type transcriptional repressor STM4068
Sulfate/thiosulfate import ATP-binding protein CysA
Beta-ketoacyl-[acyl-carrier-protein] synthase III
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Type IV secretion system coupling protein TraD DNA-binding
Mannose-1-phosphate guanylyltransferase RfbM
Reduces affinity for magnesium about 10-fold. Reduces magnesium transport by about 98%.
Reduces magnesium transport by about 95%.
Reduces magnesium transport by about 99%.
Putative 2-aminoethylphosphonate import ATP-binding protein PhnT
Interaction with host membrane and with target proteins
Phosphoglycerate transport system sensor protein PgtB
Glutamate/aspartate import solute-binding protein
Pyrimidine/purine nucleoside phosphorylase
Galactofuranosyltransferase GlfT2 N-terminal
In SS-C; sensitive to hydrophobic antibiotics and compounds.
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose
Reduces the epimerase activity by over 100-fold.
Reduces the epimerase activity by over 1000-fold.
Signal transduction histidine-protein kinase/phosphatase UhpB
Activating region 1 (AR1); probably contacts the C-terminus of RpoA
Activating region 2 (AR2); probably contacts the N-terminus of RpoA
Activating region 3 (AR3); probably contacts sigma-70 (RpoD)
In acr-4 and acr-3 respectively; higher levels of cAMP synthesis.
cAMP-activated global transcriptional regulator CRP
PTS system glucose-specific EIICB component
Abolishes formation of s(2)C32; when associated with A-122.
Abolishes formation of s(2)C32; when associated with A-125.
Ribosomal RNA large subunit methyltransferase E
Succinate dehydrogenase flavoprotein subunit
Osmoprotectant import permease protein OsmY
In parC281TS; a temperature-sensitive mutant, no change in growth rate or negative supercoiling at permissive temperature.
ECA polysaccharide chain length modulation protein
Ascorbate-specific PTS system EIIB component
Probable succinate transporter subunit YjjB
D-tagatose-1,6-bisphosphate aldolase subunit GatZ
UDP-N-acetyl-D-mannosaminuronic acid transferase
3-oxoacyl-[acyl-carrier-protein] reductase FabG
Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
Loss of the temperature-sensitive phenotype; when associated with I-125.
Loss of the temperature-sensitive phenotype; when associated with T-223.
Methionine import ATP-binding protein MetN 1
FAD-dependent cmnm(5)s(2)U34 oxidoreductase
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
DNA replication terminus site-binding protein
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
Ribosomal small subunit pseudouridine synthase A
Tetrathionate response regulatory protein TtrR
Cellulose synthase catalytic subunit [UDP-forming]
[Ribosomal protein bS18]-alanine N-acetyltransferase
Ubiquinone biosynthesis O-methyltransferase
Thiol:disulfide interchange protein DsbA
Penicillin-insensitive murein endopeptidase
Type III secretion system chaperone SseA
Iron-sulfur cluster assembly protein CyaY
Glutathione-regulated potassium-efflux system protein KefB
Autoinducer 2 import system permease protein LsrC
Phosphoenolpyruvate synthase regulatory protein
Surface presentation of antigens protein SpaR
Transcription termination/antitermination protein NusA
Hemolysin expression-modulating protein Hha
No longer interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
Anaerobic nitric oxide reductase flavorubredoxin
Ubiquinone biosynthesis accessory factor UbiK
Plays an important role in substrate specificity
Multiple antibiotic resistance protein MarA
Abolishes enzyme activity and 1.5-fold reduction of the affinity for 3-dehydroquinate.
Strong reduction of the catalytic efficiency and slight increase of the affinity for 3-dehydroquinate.
Very strong reduction of the catalytic efficiency and almost the same affinity for 3-dehydroquinate.
High-affinity branched-chain amino acid transport ATP-binding protein LivF
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Osmoprotectant import ATP-binding protein OsmV
Single-stranded-DNA-specific exonuclease RecJ
Ethanolamine ammonia-lyase large subunit
Lipopolysaccharide core heptose(II)-phosphate phosphatase
Phosphoribosylformylglycinamidine synthase
This mutant has a lower overall folding of the secondary structure and shows a 60% loss in FGAM synthase activity.
This mutant has the same secondary structure content and FGAM synthetase activity as the wild-type protein and exhibits almost no destabilization.
This mutant is extremely deleterious to the structural integrity of the protein.
This mutant is structurally identical to the wild-type protein.
This mutant shows a disturbance in the secondary structure of the protein and causes a 30% loss in FGAM synthase activity.
This mutant shows a perturbation of the local environment, however has a secondary structure content and a FGAM synthase activity very similar to the wild-type protein.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Translocation and assembly module subunit TamA
Putative multidrug resistance protein MdtD
tRNA/tmRNA (uracil-C(5))-methyltransferase
Ribosomal RNA large subunit methyltransferase K/L
No inhibition of cell growth, no degradation of tRNA(fMet).
Cobalt-precorrin-5B C(1)-methyltransferase
Altered motor bias with clockwise rotation, partially suppresses a yhjH disruption.
Penicillin-binding protein activator LpoA
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Confers to cells a PhoP constitutively active phenotype. Affects PhoP/PhoQ-signaling cascade and increases net phosphorylation of PhoP. No effect on initial rate of autophosphorylation and decreases phosphatase activity.
Confers to cells a PhoP constitutively inactive phenotype. Affects PhoP/PhoQ-signaling cascade but no significantly effect on net phosphorylation of PhoP. Decreases 3-fold initial rate of autophosphorylation and increases phosphatase activity.
Confers to cells a PhoP wild-type phenotype. No effect on net phosphorylation of PhoP. Decreases 5-fold initial rate of autophosphorylation and increases phosphatase activity.
Confers to cells a PhoP wild-type phenotype. No effect on net phosphorylation of PhoP. Decreases 5-fold initial rate of autophosphorylation and shows a wild-type phosphatase activity.
Impaired PhoP repression by high concentrations of divalent cation.
Impaired PhoP repression by high concentrations of divalent cations.
In pho-24; low affinity for Ca(2+). Confers to cells a PhoP constitutively active phenotype. Affects PhoP/PhoQ-signaling cascade and increase net phosphorylation of PhoP. No effect on initial rate of autophosphorylation and decreases phosphatase activity.
Plays a critical role in the switching between kinase and phosphatase states
Retains a wild-type Mg(2+) response only at 10 mM in strain PhoP* PhoQ expressing mutant phoPN-93. Loss of autophosphorylation, irrespective of the presence of Mg(2+). Unable to promote phoP dephosphorylation even in presence of added Mg(2+).
Zinc-regulated secreted antivirulence protein C-terminal
In parE206TS; a temperature-sensitive mutant, 33% decreased growth rate, slightly decreased negative supercoiling at permissive temperature.
In temperature-sensitive mutant parE377.
In temperature-sensitive mutant parE493.
In temperature-sensitive mutants parE206 and parE374.
NADP-dependent 3-hydroxy acid dehydrogenase YdfG
Carbamoyl-phosphate synthase large chain
Abolishes plasmid resolution but not chromosomal recombination.
Enzyme associates with BMCs, 25% decrease in enzyme activity.
Malonyl-[acyl-carrier protein] O-methyltransferase
Cytochrome c biogenesis ATP-binding export protein CcmA 1
Essential for degradation by Lon protease
Thiol:disulfide interchange protein DsbL
Oxaloacetate decarboxylase gamma chain 3
HupH hydrogenase expression protein C-terminal
Oxygen-independent coproporphyrinogen III oxidase
Molybdopterin synthase catalytic subunit
Gamma-glutamylcyclotransferase AIG2-like
Peptide transport periplasmic protein SapA
1-acyl-sn-glycerol-3-phosphate acyltransferase
sn-glycerol-3-phosphate-binding periplasmic protein UgpB
Iron(3+)-hydroxamate import system permease protein FhuB
SPI-1 type 3 secretion system translocon protein SctE
Ribosomal large subunit pseudouridine synthase B
Aromatic amino acid beta-eliminating lyase/threonine aldolase
Probable [Fe-S]-dependent transcriptional repressor
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
N-acetyl-gamma-glutamyl-phosphate reductase
Heme-copper oxidase subunit III family profile
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF
Surface presentation of antigens protein SpaK
Trk system potassium uptake protein TrkA
3-phosphoshikimate 1-carboxyvinyltransferase
UDP-N-acetylenolpyruvoylglucosamine reductase
ATP-dependent Clp protease adapter protein ClpS
Ubiquinone biosynthesis accessory factor UbiJ
Sulfite reductase [NADPH] hemoprotein beta-component
Bacterial microcompartment shell protein EutK
Methyl-accepting chemotaxis citrate transducer
4-hydroxy-tetrahydrodipicolinate reductase
3-deoxy-manno-octulosonate cytidylyltransferase
Bacterial type II secretion system protein E
Glycosyl hydrolase family 30 beta sandwich
23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD
Tetrathionate sensor histidine kinase TtrS
Bacterial microcompartment shell protein EutM
No longer complements pduA in the pdu operon.
Multiple antibiotic resistance protein MarB
Putative polysaccharide export protein wza
Defective in mismatch repair; ATPase activity reduced 6-fold.
Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP
tRNA(Met) cytidine acetyltransferase TmcA
Ribosomal large subunit pseudouridine synthase E
Aminoalkylphosphonate N-acetyltransferase
Cobalt-precorrin-4 C(11)-methyltransferase
L-carnitine/gamma-butyrobetaine antiporter
Putative glutamine amidotransferase-like protein YfeJ
Nucleophile; methyl group acceptor from phosphotriester
Bacterial microcompartment shell vertex protein PduN
NADPH-dependent 7-cyano-7-deazaguanine reductase
2-amino-3-ketobutyrate coenzyme A ligase
BMCs have a severe assembly defect, makes about 27% BMCs.
Bacterial microcompartment shell protein PduB
Does not make PduB', makes about 66% BMCs, with wild-type appearance.
Makes about 25% BMCs, 35% diol dehydratase content.
Makes about 70% BMCs, 74% diol dehydratase content.
Makes about 80% BMCs, 78% diol dehydratase content.
Makes about 90% BMCs, 87% diol dehydratase content.
Makes large empty BMCs, makes about 11% BMCs.
Makes large empty BMCs, makes about 30% BMCs.
Probable helix that binds cargo to the BMC shell
In mutants SL1102 and SL1027; heptoseless LPS.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
SPI-2 type 3 secretion system needle filament protein
Decreases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2.
Increases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2.
Partial reduction in the peripheral accumulation of GFP-PipB2 and LAMP-1.
Prevents LE/Lys redistribution. Does not affect the peripheral accumulation of GFP-PipB2.
Prevents the peripheral accumulation of GFP-PipB2 and LAMP-1. Slightly affects its LE/Lys redistribution.
Aminoglycoside N(6')-acetyltransferase type 1
Surface presentation of antigens protein SpaN
Bifunctional purine biosynthesis protein PurH
Ethanolamine ammonia-lyase small subunit
Lipopolysaccharide 1,6-galactosyltransferase
Increases speed of forward and back reactions by 2-3 fold.
Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH.
Slightly decreases speed of forward and back reactions.
Confers streptomycin independence on streptomycin-dependent S12 mutant P90R. ARAM mutant.
Confers streptomycin independence on streptomycin-dependent S12 mutant P91D. ARAM mutant.
In JB221; fast-growing suppressor of streptomycin resistant mutant S12 K42N. ARAM mutant.
Streptomycin resistant. Also confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
Streptomycin resistant. Also confers streptomycin independence on streptomycin-dependent S12 mutant P91D.
Oxaloacetate decarboxylase gamma chain 1
Curli production assembly/transport component CsgG
D-galactose/methyl-galactoside binding periplasmic protein MglB
Interacts with membrane-bound trg signal transducer
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
NAD-dependent dihydropyrimidine dehydrogenase subunit PreT
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase
Cobalt-precorrin-7 C(5)-methyltransferase
Carbamoyl-phosphate synthase small chain
Periplasmic oligopeptide-binding protein
Could play a key role in the communication between the regulatory and the substrate sites
Thiol:disulfide interchange protein DsbD
Branched-chain-amino-acid aminotransferase
Electron transfer via tryptophanyl radical
Protein-glutamate methylesterase/protein-glutamine glutaminase
Slight increase in methylesterase activity.
Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)
Salmonella pathogenicity island 2 protein C
Glutathione import ATP-binding protein GsiA
Glycoside hydrolase family 31 N-terminal
Ascorbate-specific PTS system EIIC component
Protein-L-isoaspartate O-methyltransferase
Bacterial microcompartment shell protein PduK
Peptidase S9 prolyl oligopeptidase catalytic
Bifunctional adenosylcobalamin biosynthesis protein CobU
No activity; when associated with Ala-46.
No activity; when associated with Ala-47.
HTH-type DNA-binding transcriptional activator EutR
Lipopolysaccharide heptosyltransferase 1
sn-glycerol-3-phosphate transport system permease protein UgpA
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
N-succinylglutamate 5-semialdehyde dehydrogenase
Involved in high-affinity binding for sodium and proline
D-methionine transport system permease protein MetI
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
Almost loss of activity. Decreases helical content of the protein.
Strong decrease in activity. Small decrease in the helical content of the protein.
Bacterial microcompartment shell vertex protein EutN
Deoxyguanosinetriphosphate triphosphohydrolase
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Pyrimidine-specific ribonucleoside hydrolase RihA
Oligopeptide transport ATP-binding protein OppD
Anaerobic nitric oxide reductase transcription regulator NorR
Uncharacterized inner membrane transporter YicL
Autoinducer 2 import ATP-binding protein LsrA
4-hydroxythreonine-4-phosphate dehydrogenase
Nucleophile; cysteine thiosulfonate intermediate
Phosphoadenosine 5'-phosphosulfate reductase
Major facilitator superfamily associated
Putative 2,3-dihydroxypropane-1-sulfonate exporter
DNA protection during starvation protein
Loss of ubiquitin ligase activity. No thioester formation.
NADH:ubiquinone oxidoreductase 30kDa subunit
Probable transcriptional regulatory protein YebC
3-isopropylmalate dehydratase large subunit 1
Involved in allosteric activation by GTP
Probable 2-(5""-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase 1
The 3 Arg may form a positively charged pocket, which binds the alpha-carboxyl group of the attractant AA
Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.
Decreases arginine and histidine binding affinity.
Has no effect on the arginine and histidine binding affinity.
Lysine/arginine/ornithine-binding periplasmic protein
Shows a drastic decrease in arginine and histidine binding affinity.
Bacterial microcompartment shell protein PduA
Crystallized, central pore can be open or occluded, less permeable to substrate, cells grow slowly on 1,2-PD.
Crystallized, central pore is less symmetric, no change in shell permeability, increased permeability to glycerol, wild-type growth on 1,2-PD.
Crystallized, central pore is more hydrophobic but same size, less permeable to PD, 50% DDH activity, cells grow slowly on 1,2-PD.
Crystallized, central pore is occluded, less permeable to PD, 62% DDH activity, cells grow slowly on 1,2-PD.
Crystallized, central pore is occluded, less permeable to substrate, 75% DDH activity, cells grow slowly on 1,2-PD.
Decreased amounts of PduP in purified BMCs.
Improved growth on 1,2-PD, makes slightly larger BMCs, alters accumulation of PD metabolites.
No BMCs are made, forms hexamers which do not form arrays, dominant to wild-type protein. Little to no growth on 1,2-PD, cells elongate and are joined by filaments, BMC proteins aggregate near poles. No longer forms filaments upon overexpression, does not restore BMC formation to a double pduA-pduJ deletion. Does not form closed shells.
No change in shell permeability to PD, excretes more propionaldehyde, wild-type growth on 1,2-PD.
No change in shell permeability, wild-type growth on 1,2-PD.
Slow growth at limiting vitamin B12, wild-type at saturating conditions.
Subject to propionaldehyde toxicity, makes about 70% BMCs, protein shells appear wild-type but leak.
Subject to propionaldehyde toxicity, makes about 75% BMCs, shells are wrinkled and leaky.
Ribonucleoside-diphosphate reductase 2 subunit alpha
Potassium-transporting ATPase potassium-binding subunit
Restriction system protein Mrr-like N-terminal
Voltage-gated ClC-type chloride channel ClcB
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Resistance to polyketide antibiotic thailandamide.
Gly-cisPro motif, important for rejection of L-amino acids
Phosphate import ATP-binding protein PstB
N-(5'-phosphoribosyl)anthranilate isomerase
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Uncharacterized MFS-type transporter YhhS
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Glutathione transport system permease protein GsiD
Bacterial microcompartment assembly protein PduM
D-tagatose-1,6-bisphosphate aldolase subunit GatY
Peptide methionine sulfoxide reductase MsrA
Contains three zinc binding sites, but the third site shows a decreased affinity for zinc.
Flagellar transcriptional regulator FlhC
Tellurite resistance methyltransferase TehB-like
UDP-N-acetyl-D-mannosamine dehydrogenase
Alpha-D-phosphohexomutase alpha/beta/alpha
Reduces the ability to hydrolyze Asp-Leu without decreasing its ability to hydrolyze Leu-Leu.
4-hydroxy-tetrahydrodipicolinate synthase
High-affinity branched-chain amino acid transport system permease protein LivH
Ribosomal protein uL3 glutamine methyltransferase
Probable transcriptional regulatory protein YeeN
Oligopeptide transport system permease protein OppB
Phosphoribosylformylglycinamidine cyclo-ligase
Vitamin B12 import system permease protein BtuC
Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ
Glycosyl transferase family 3 N-terminal
Uncharacterized MFS-type transporter STM4428
Putative 2-aminoethylphosphonate transport system permease protein PhnU
10-fold reduction in amidation activity with glutamine as substrate. The affinity for cobyrinate is nearly not affected whereas that for the c-monoamide intermediate decreases by 6-fold.
26-fold reduction in amidation activity with glutamine as substrate. The affinity for cobyrinate is nearly not affected whereas that for the c-monoamide intermediate decreases by 27-fold.
3-fold reduction in amidation activity with glutamine as substrate.
500-fold reduction in amidation activity with either glutamine or ammonia as substrate.
Cobyrinate a,c-diamide synthase, N-terminally processed
Increases nucleophilicity of active site Cys
Loss of amidation activity when glutamine is used as substrate, and 750-fold reduction in amidation activity with ammonia as substrate.
Loss of amidation activity with either glutamine or ammonia as substrate.
Ribosomal protein bS6--L-glutamate ligase
Phosphoglycerate transport system transcriptional regulatory protein PgtA
Thiosulfate reductase cytochrome B subunit PhsC
Polysaccharide chain length determinant N-terminal
2-aminoethylphosphonate--pyruvate transaminase
Decreased affinity for all of the substrates.
RNA methyltransferase SpoU/TrmH type C-terminal
DNA-binding dual transcriptional regulator OmpR
NADH-rubredoxin oxidoreductase C-terminal
Nitrite/Sulfite reductase ferredoxin-like
Homoserine/homoserine lactone efflux protein
Bacterial microcompartment shell protein PduJ
Produces amorphous bodies instead of BMCs. Reduces crystallographic problems that result from edge-to-edge aggregation of BMC hexamers. No longer forms filaments upon overexpression, does not restore BMC formation to a double pduA-pduJ deletion.
Wild-type growth rate, BMCs appear normal, wild-type DDH activity. In the chromosomal position of pduA, less permeable to 1,2-PD, 65% DDH activity, cells grow slower on 1,2-PD.
Ribonucleoside-diphosphate reductase 1 subunit beta
SPI-1 type 3 secretion system translocon protein SctB
Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
DNA polymerase III delta subunit C-terminal
Flagellar hook-basal body complex protein FliE
N-acetylmuramic acid 6-phosphate etherase
Abolishes function, decreased autophosphatase activity.
Abolishes function, reduced rate of phosphorylation and affinity for magnesium ion.
Responsible for conferring strict specificity to NAD
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
Bacterial microcompartment shell protein PduU
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Ribosomal RNA small subunit methyltransferase J
Capsule biosynthesis GfcC-like C-terminal
Capsule biosynthesis GfcC-like N-terminal
Almost completely abrogates the secretion of SptP and also affects the secretion of early (InvJ/SctP) and middle (SipB/SctE) substrates.
Can still secrete the T3SS components early substrates (InvJ/SctP), middle substrates (SipB/SctE, SipC/SctB and SipD/SctA), but shows a marked defect in the secretion of the effector proteins SptP and SopB (late substrates). Secretion of the effectors SipA and SopE2 is only marginally reduced.
Exists predominantly as monomer in solution and can self-associates into dimers.
Lack of ATPase activity. Defective in type III secretion and invasion of cultured cells.
Lack of ATPase activity. Exhibits a decreased ability to form oligomers. Defective in type III secretion and invasion of cultured cells.
Lack of ATPase activity. Severely defective for the secretion of early (InvJ/SctP), middle (SipB/SctE) and late (SptP) substrates.
Retains wild-type levels of ATPase activity. Defective in type III secretion and invasion of cultured cells.
Retains wild-type levels of ATPase activity. Defective in type III secretion and invasion of cultured cells. Does not bind the SicP-SptP complex.
Retains wild-type levels of ATPase activity. Drastically impaired in its ability to associate with the membrane. Defective in type III secretion and invasion of cultured cells.
Shows a defect in the secretion of SptP but not in the secretion of early (InvJ/SctP) or middle (SipB/SctE) substrates.
Loss of ATP hydrolysis and autophosphorylation.
Uncharacterized MFS-type transporter YfcJ
Kdo(2)-lipid A phosphoethanolamine 7""-transferase
Plays a key role in the stabilization of the transition state and positioning the aldehyde component
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Phenylalanine--tRNA ligase alpha subunit
ATP-dependent protease ATPase subunit HslU
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Ribosomal RNA small subunit methyltransferase A
Flagella basal body P-ring formation protein FlgA
Potassium-transporting ATPase ATP-binding subunit
Cytochrome c biogenesis ATP-binding export protein CcmA 2
Thiosulfate reductase molybdopterin-containing subunit PhsA
Probable 2-(5""-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase 2
Redox-sensitive transcriptional activator SoxR
Low level streptomycin, spectinomycin and kanamycin resistance, has small colony phenotype.
Probable amino-acid metabolite efflux pump
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
Vitamin B6 salvage pathway transcriptional repressor PtsJ
p-hydroxybenzoic acid efflux pump subunit AaeA
Ion-translocating oxidoreductase complex subunit C
Prepilin peptidase dependent protein C-like C-terminal
Putative N-acetylmannosamine-6-phosphate 2-epimerase 2
Aspartate/homoserine dehydrogenase NAD-binding
Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252)
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-105)
Could be important to modulate the pK values of the two catalytic cysteine residues
Alpha-ketoglutarate-dependent dioxygenase AlkB
Imidazole glycerol phosphate synthase subunit HisH
Outer membrane protein assembly factor BamE
Spermidine/putrescine import ATP-binding protein PotA
Schiff-base intermediate with acetaldehyde
Voltage-dependent anion channel-forming protein YneE
5-glutamyl coenzyme A thioester intermediate