Interaction with polymerase core subunit RpoC
Transposase IS116/IS110/IS902 C-terminal
Part of the proton transportation network
Part of the proton-transportation channel
50-fold reduction in activity and slight reduction in the affinity for beta-alanine. 30- and 40-fold decrease in adenylate formation and pantothenate formation, respectively.
More than 1000-fold reduction in activity and 120-fold decrease in adenylate formation. The enzymatic activity and the affinity for beta-alanine can be increased 10- and 3-fold, respectively, by alkylation of cysteine of mutant C-160.
More than 1000-fold reduction in activity and 45-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 50-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 50-fold decrease in the affinity for ATP.
More than 1000-fold reduction in activity and 52-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 60-fold decrease in adenylate formation. 10-fold decrease in the affinity for ATP.
Glutamyl-tRNA(Gln) amidotransferase subunit C
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Cysteine persulfide intermediate; for sulfurtransferase activity
Glycyl thioester intermediate; for adenylyltransferase activity
Probable adenylyltransferase/sulfurtransferase MoeZ
GDP-mannose-dependent alpha-mannosyltransferase
Probable soluble pyridine nucleotide transhydrogenase
Important for determining product chain length
Leads to the appearance of shorter chain products, GPP and E,E-FPP, with only small amounts of GGPP produced.
HTH-type transcriptional regulator Rv0880
14-fold reduction in catalytic activity. 10-fold decrease in affinity for ATP but no change in affinity for UTP. Causes resistance to the anti-tubercular compounds 7947882 and 7904688. Catalytic activity cannot be inhibited by the active metabolite of 7947882.
Characteristic for family members without dihydropteroate synthase activity
Decreases activity and alters substrate specificity.
Putative maltooligosyl trehalose synthase
Trehalose transport system permease protein SugA
Seven-bladed beta-propeller protein Rv1057
Major facilitator superfamily (MFS) profile
Salicyl-AMP ligase / salicyl-S-ArCP synthetase
Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Loss of more than 70% enzymatic activity.
Uncharacterized formamidopyrimidine-DNA glycosylase-like protein
Putative HTH-type transcriptional regulator Rv0890c
Uncharacterized glycosyltransferase Rv0539
Multidrug efflux system ATP-binding protein Rv1218c
Blue shift in fluorescence emission, possible decrease in -10 promoter recognition.
Nucleophile; cysteine thiosulfonate intermediate
Ribosomal RNA small subunit methyltransferase G
No effect on PbpB (PBP3, FtsI) processing.
Uncharacterized PPE family protein PPE23
Solute-binding protein family 3/N-terminal
Acyl-CoA dehydrogenase/oxidase C-terminal
Uncharacterized PPE family protein PPE46
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3399
(3R)-3-[(carboxymethyl)amino]fatty acid oxygenase/decarboxylase
Fluoroquinolones export ATP-binding protein Rv2688c
Resolvase/invertase-type recombinase catalytic
F420H(2)-dependent quinone reductase Rv1261c
Uncharacterized HTH-type transcriptional regulator Rv2912c
Uncharacterized MFS-type transporter Rv0849
Succinate--CoA ligase [ADP-forming] subunit beta
Pyridoxamine 5'-phosphate oxidase putative
Unable to dehydrogenate pregnene-carboxyl-CoA ester.
12-fold decrease in catalytic efficiency with NADH as substrate. 26-fold decrease in catalytic efficiency; when associated with A-146.
2.2-fold decrease in catalytic efficiency with NADH as substrate. 26-fold decrease in catalytic efficiency; when associated with A-445.
3-oxoacyl-[acyl-carrier-protein] reductase [NADH]
Drastic reduction of ketoreductase activity.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0731c
Molybdopterin synthase sulfur carrier subunit
3-hydroxyacyl-CoA dehydrogenase C-terminal
3-hydroxyacyl-CoA dehydrogenase NAD binding
Probable peptidoglycan biosynthesis protein MviN
Uncharacterized HTH-type transcriptional regulator Rv0767c
Does not inhibit growth, forms complex with VapB4.
Diacylglycerol acyltransferase/mycolyltransferase Ag85C
Do not form alpha,alpha-trehalose dimycolate (TDM, cord factor) and alpha,alpha-trehalose monomycolate (TMM).
NADH-rubredoxin oxidoreductase C-terminal
Nitrite/Sulfite reductase ferredoxin-like
Probable protein-export membrane protein SecG
Uncharacterized HTH-type transcriptional regulator Rv1395
F420H(2)-dependent quinone reductase Rv1558
Decreased ability to bind dsDNA, unwinds but does not collapse cosmid DNA.
6-phosphogluconate dehydrogenase C-terminal
(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase
Probable hercynylcysteine sulfoxide lyase
Putative trans-acting enoyl reductase Rv2449c
Decreased TLR2 agonist activity, cannot acquire lipid from mycobacterial extracts, cavity entrance and size decrease. Binds PIM6, LM and ManLAM 10-100-fold less well than wild-type, binds de-acylated LM and ManLAM as well as wild-type. Decreased ability to transfer triacylglyceride between lipid bilayers.
Prevents bacterial uptake by a human macrophage-like cell line
Sec translocon accessory complex subunit YajC
About 5% of wild-type phosphatase activity.
Almost completely abolished phosphatase activity.
Disrupts dimerization of the enzyme, which exists as a monomer and has lost its ability to perform dephosphorylation.
Loss of GTP-synthesizing activity and lack of autophosphorylation. Does not interact with Ndk.
Partial loss of GTP-synthesizing activity and decrease in autophosphorylation. Loss of GTP-synthesizing activity and lack of autophosphorylation; when associated with A-115.
Partial loss of GTP-synthesizing activity and decrease in autophosphorylation. Loss of GTP-synthesizing activity and lack of autophosphorylation; when associated with A-247.
Partial loss of GTP-synthesizing activity.
Ribonucleoside-diphosphate reductase subunit alpha
Uncharacterized ABC transporter ATP-binding protein Rv0073
Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins); alternate
Abolishes interaction with Rv1747. Impairs autokinase activity.
Decreases interaction with Rv1747. Decreases autokinase activity.
Loss of kinase activity. Abolishes interaction with Rv1747.
Contributes to the formation of an oxyanion binding site for the epoxide oxygen of substrate
Plays an orienting role for the imidazole group of His-333
Cysteine sulfenic acid (-SOH) intermediate
Interchain (with C-61 in AhpC); in linked form
Loss of activity. Loss of interchain disulfide bond with AhpC.
Reduces protein stability. No effect on catalytic activity.
Hypoxanthine-guanine phosphoribosyltransferase
Probable cytochrome c oxidase polypeptide 4
Uncharacterized ABC transporter ATP-binding protein Rv1281c
Acyl-ester intermediate; for transpeptidase activity
Proton donor; for transglycosylase activity
ABC-type glycine betaine transport system substrate-binding
Probable cystathionine beta-synthase Rv1077
Transposase for insertion sequence element IS1557
Loss of autophosphorylation. Partially complements an M.smegmatis mtrB disruption, cells are still filamentous and bulged but the protein targets to septa.
Acyl-CoA dehydrogenase/oxidase N-terminal
Required for the condensation reaction. Not required to bind substrate
Probable 3-hydroxyisobutyrate dehydrogenase
Probable transport accessory protein MmpS1
Putative FAD-containing monooxygenase MymA
Can form a disulfide bond; when associated with C-440. Decreased autophosphorylation; when associated with C-524. Loss of autophosphorylation; when associated with C-440 or C-440 and C-524.
Can form a disulfide bond; when associated with C-537. Loss of autophosphorylation; when associated with C-524 or C-524 and C-537.
Decreased autophosphorylation when CO- or NO-bound, none in the deoxy or O(2)-bound state, protein more easily oxidized to Fe(3+) state.
Isolated kinase core no longer forms dimers, autophosphorylation unaffected. Decreased autophosphorylation; when associated with C-537. Loss of autophosphorylation; when associated with C-440 or C-440 and C-537.
No autophosphorylation activity no matter the bound gaseous ligand, protein more easily oxidized to Fe(3+) state.
No autophosphorylation activity no matter the bound gaseous ligand.
No autophosphorylation when Fe(2+) protein is bound to CO or NO; no change in autophosphorylation of deoxy-protein. No change in auto-oxidation, slightly higher affinity for O(2) and CO.
No autophosphorylation, no transfer to DevR (DosR).
No autophosphorylation. Isolated kinase core binds ATP.
No change in autophosphorylation when NO-bound, decreased autophosphorylation in deoxy or CO-bound state, slightly increased activity in O(2)-bound state.
No change in autophosphorylation when NO-bound, loss of autophosphorylation in deoxy or CO-bound state.
No change in autophosphorylation when deoxy or NO-bound, decreased autophosphorylation when CO-bound, increased activity in O(2)-bound state.
Oxygen sensor histidine kinase response regulator DevS/DosS
Phosphomannose isomerase type I catalytic
HTH-type transcriptional regulator Mce2R
Probable fatty acid methyltransferase Rv3720
Probable phosphatidylglycerophosphate synthase
Loss of phosphorylation; when associated with A-168.
Loss of phosphorylation; when associated with A-183.
Alpha-ketoglutarate-dependent sulfate ester dioxygenase
No in vitro phosphorylation by PknB; when associated with A-106.
No in vitro phosphorylation by PknB; when associated with A-26.
Uncharacterized PPE family protein PPE30
Uncharacterized HTH-type transcriptional regulator Rv0472c
Does not affect interaction with NCF2 and ROS production.
Does not interact with NCF2 and cannot inhibit ROS production.
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Cell envelope-related transcriptional attenuator
Involved in Mg(2+) ion dislocation from EF-Tu
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3767c
Uncharacterized PPE family protein PPE33
Probable histone acetyltransferase Rv0428c
7-fold increase in holoenzyme adenylyl cyclase activity at pH 8.0.
Almost complete loss of adenylyl cyclase activity in the catalytic fragment.
Loss of adenylyl cyclase activity in the catalytic fragment.
Retains 10% adenylyl cyclase activity in the catalytic fragment.
HTH-type transcriptional regulator Rv1931c
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-23; A-56 and A-62.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-33; A-53 and A-56.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-33; A-53 and A-62.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-53; A-56 and A-62.
Redox- and pH-responsive transcriptional regulator WhiB3
Abolishes catalytic activity on AG substrates.
Abolishes catalytic activity on AG substrates. Abolishes catalytic activity on HupB.
Decreases catalytic activity on AG substrates, leads to a change in the number of acetylated sites.
Nearly abolishes catalytic activity on AG substrates.
No effect on catalytic activity on AG substrates. Prevents artifactual CoA adduct formation during crystallization.
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
UDP-N-acetylenolpyruvoylglucosamine reductase
Uncharacterized MFS-type transporter Rv2456c
Could have a critical role in the catalytic mechanism
Small but detectable amount of cYY-forming activity.
Small but detectable amount of catalytic activity (200-fold decrease).
Enhances catalytic activity and stability.
NADPH-dependent stearoyl-CoA 9-desaturase
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM
Arabinogalactan biosynthesis recruiting protein Rv3789
Uncharacterized PPE family protein PPE63
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase
4-fold decrease in affinity for acylated mas protein. No change in catalytic activity.
40-fold decrease in affinity for acylated mas protein. No change in catalytic activity.
Important for mas ACP domain recognition
Less than 1.9-fold decrease in activity.
Phthiocerol/phthiodiolone dimycocerosyl transferase
Structural role in the organization of the active site
Uncharacterized PPE family protein PPE66
Deoxyguanosinetriphosphate triphosphohydrolase-like protein
F420-dependent hydroxymycolic acid dehydrogenase
Abolishes phosphorylation. No loss of DNA-binding activity in vitro.
Methylated-DNA--protein-cysteine methyltransferase
HTH-type transcriptional regulator Rv1816
Phosphoribosylformylglycinamidine synthase subunit PurL
Ribosomal RNA adenine methylase transferase N-terminal
Putative diacyglycerol O-acyltransferase Rv3480c
Causes a decrease in ammonia channel efficiency to 70% compared with wild-type enzyme.
Lack of glutamine-dependent activity. Retains 30% of ammonia-dependent activity.
Lack of glutamine-dependent activity. Retains 90% of ammonia-dependent activity.
Lack of glutamine-dependent and ammonia-dependent activities.
Proton acceptor; for glutaminase activity
Acyl-ester intermediate; for acyltransferase activity
Acyl-thioester intermediate; for beta-ketoacyl synthase activity
Cannot form alpha-alkyl beta-ketoacids derivatives.
Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase
Protein no longer targeted to encapsulin nanocompartments, retains enzyme activity.
Loss of activity, cannot rescue a deletion mutant.
3-oxoacyl-[acyl-carrier-protein] synthase 1
Interacts with the inhibitor thiolactomycin
Mimics structural changes caused by acyl-enzyme formation.
UDP-glucose/GDP-mannose dehydrogenase C-terminal
Putative regulatory protein FmdB Zinc ribbon
Reduces lipoamide dehydrogenase activity by 82%.
Reduces lipoamide dehydrogenase activity by 89%.
Reduces lipoamide dehydrogenase activity by 91%.
Reduces lipoamide dehydrogenase activity by 94%.
Reduces lipoamide dehydrogenase activity by 95%.
Reduces lipoamide dehydrogenase activity by 96%.
ESX-3 secretion-associated protein EspG3
20% ligase activity for singly nicked dsDNA, normal N6-AMP-lysine intermediate formed, no effect on phosphodiester bond formation, no effect on DNA polymerase.
Better than wild-type DNA-binding and filling on single nt gaps, impaired gap filling on more complicated templates (in Pol domain).
Binds DNA, no formation of DNA end-bridging complex, no polymerase activity. Significantly decreased ability to fill in 2 nt gaps (in Pol domain).
Binds DNA, no gap-filling (in Pol domain).
DNA repair polymerase domain (Pol); interacts with Ku
Loss of DNA-binding, no polymerase activity, no effect of gap-filling (in Pol domain).
Loss of adenyltransferase activity, no N6-AMP-lysine formation and loss of ligase activity. No effect on phosphodiester bond formation on pre-adenylated nicked DNA, or on DNA polymerase.
Loss of polymerase activities, no DNA repair (in Pol domain).
Multifunctional non-homologous end joining DNA repair protein LigD
N6-AMP-lysine intermediate; for ligase activity
No ligase of singly nicked dsDNA activity, 25% N6-AMP-lysine intermediate formed, decreased phosphodiester bond formation, no effect on DNA polymerase.
No ligase of singly nicked dsDNA activity, no N6-AMP-lysine intermediate formed, decreased phosphodiester bond formation on pre-adenylated nicked DNA, no effect on DNA polymerase.
No ligase of singly nicked dsDNA activity, no N6-AMP-lysine intermediate formed, no phosphodiester bond formation on pre-adenylated nicked DNA, no effect on DNA polymerase.
On substrate with 5'-phosphate, 1 base pair (bp) complementarity and 1 nucleotide (nt) gap, greatly reduced DNA-binding and gap-filling. Barely detectable activity on substrate with high complementarity and 3'-overhang (in Pol domain).
On substrate with 5'-phosphate, 1 bp complementarity and 1 nt gap, greatly reduced DNA-binding and gap-filling. Barely detectable activity on substrate with high complementarity and 3'-overhang (in Pol domain).
On substrate with 5'-phosphate, 1 bp complementarity and 1 nt gap, greatly reduced DNA-binding and gap-filling. No activity on substrate with high complementarity and 3'-overhang (in Pol domain).
Transition state stabilizer; for 3'-phosphoesterase activity
Wild-type filling on single nt gaps, impaired gap filling on more complicated templates (in Pol domain).
Decrease in activity; when associated with A-106; A-109 and A-128.
Decrease in activity; when associated with A-106; A-109 and A-131.
Decrease in activity; when associated with A-106; A-128 and A-131.
Decrease in activity; when associated with A-109; A-128 and A-131.
Decreases inhibition by AX20017; when associated with S-87.
Decreases inhibition by AX20017; when associated with S-92.
Lack of activity; when associated with S-106; S-109 and S-128.
Lack of activity; when associated with S-106; S-109 and S-131.
Lack of activity; when associated with S-106; S-128 and S-131.
Lack of activity; when associated with S-109; S-128 and S-131.
Lack of phosphorylation. Does not affect kinase activity.
tRNA (adenine(58)-N(1))-methyltransferase TrmI
Bifunctional F420 biosynthesis protein FbiB
Loss of activity; when associated with A-179.
Loss of activity; when associated with A-181.
Adopts a conformation almost identical to the wild-type.
3-oxoacyl-[acyl-carrier-protein] reductase MabA
70% decrease in activity with acetoacetyl-CoA as substrate. Does not affect NADP binding.
Complete protein inactivation and freezes the catalytic site into its closed form. Totally inactive; when associated with V-60 and L-144.
Displays a lower activity than the wild-type and a slightly decreased affinity for the cofactor. Retains 84% of activity; when associated with L-144. Totally inactive; when associated with A-139 and L-144.
Loss of activity. Impaired NADPH binding.
Phosphomimetic mutant that retains less than 10% of wild-type reductase activity. Impaired NADPH binding. Overproduction of the mutant leads to a significant inhibition of de novo biosynthesis of mycolic acids.
Recombinant protein expressed in E.coli.
Retains 22% of wild-type reductase activity. Strong decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-114.
Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-114 and A-191.
Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-191.
Stabilizes the catalytic loop in its open active form. Retains 84% of activity; when associated with V-60. Totally inactive; when associated with V-60 and A-139.
Mycothiol-dependent maleylpyruvate isomerase metal-binding
Decaprenyl-phosphate phosphoribosyltransferase
No DPPR synthase activity and the protein is not expressed in the membrane.
Only small effects on the DPPR synthase activity.
Substantially reduced the DPPR synthase activity.
The DPPR synthase activity is strongly diminished.
Ribosomal RNA small subunit methyltransferase I
4-hydroxy-tetrahydrodipicolinate synthase
Exists as dimer in solution. Has similar activity to the wild-type enzyme. Slight decrease in affinity for substrates.
Loss of DNA-binding, in fragment 66-112.
Loss of activity. Can form dimers but does not bind DNA.
No change in activity. Can still bind DNA and form dimers.
Reduced DNA-binding, in fragment 66-112.
Uncharacterized PE-PGRS family protein PE_PGRS54
Uncharacterized protein Rv2386A/RVBD_2386A
Decrease in FAD-binding and loss of activity.
Mycobactin import ATP-binding/permease protein IrtA
No change in FAD-binding and in activity.
Cytochrome C biogenesis protein transmembrane
14.5-fold decrease in catalytic efficiency.
24-fold decrease in catalytic efficiency.
3.1-fold decrease in catalytic efficiency.
3.5-fold decrease in catalytic efficiency.
4.4-fold decrease in catalytic efficiency.
6.6-fold decrease in catalytic efficiency.
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
RNA 2-O ribose methyltransferase substrate binding
Beta-dihydromenaquinone-9 omega-hydroxylase
17-fold increased resistance to fluoroquinolones, 4- to 8-fold reduction in fluoroquinolone-induced DNA cleavage.
25- 45-fold increased resistance to fluoroquinolones, 4- to 8-fold reduction in fluoroquinolone-induced DNA cleavage. Supercoiling, relaxation, decatenation activities no longer inhibited by MfpA.
4- to 16-fold more efficient in fluoroquinolone-induced DNA cleavage alone. Hypersusceptibile, 2- to 14-fold higher sensitivity to fluoroquinolones, 2- to 8-fold more efficient in fluoroquinolone-induced DNA cleavage; when associated with A-80.
80-fold increased resistance to fluoroquinolones, 32- to 64-fold reduction in fluoroquinolone-induced DNA cleavage.
Confers ciprofloxacin resistance, in clinical isolate.
Confers fluoroquinolone resistance, IC(50) is 2- to 26-fold higher than wild-type.
Confers fluoroquinolone resistance, IC(50) is 3- to 43-fold higher than wild-type, in strains H37Ra and H37Rv.
Increased susceptibility to fluoroquinolones (makes sequence more like E.coli), supercoiling, relaxation, decatenation activities still inhibited by MfpA.
No change in supercoiling activity, wild-type decatenation or fluoroquinolone-induced DNA cleavage.
No supercoiling activity, almost wild-type decatenation activity, wild-type fluoroquinolone-induced DNA cleavage.
No supercoiling activity, wild-type decatenation and fluoroquinolone-induced DNA cleavage.
No supercoiling or decatenation activity, decreased fluoroquinolone-induced DNA cleavage.
O-(5'-phospho-DNA)-tyrosine intermediate
Reduced supercoiling activity, wild-type decatenation and fluoroquinolone-induced DNA cleavage.
Significant reduction in DNA wrapping and supercoiling activity, no change in decatanation or relaxation activities.
Slight reduction in supercoiling activity.
Slight resistance to fluoroquinolones. Hypersusceptibile, 2- to 14-fold higher sensitivity to fluoroquinolones, 2- to 8-fold more efficient in fluoroquinolone-induced DNA cleavage; when associated with G-90.
Wild-type decatenase activity (changes residue to match E.coli).
Wild-type decatenase activity (changes residues to match E.coli).
Wild-type supercoiling, decatenation and fluoroquinolone-induced DNA cleavage.
Sec-independent protein translocase protein TatA
Doxorubicin resistance ATP-binding protein DrrA
Putative Rieske 2Fe-2S iron-sulfur protein Rv3818
Loss of interaction with the proteasome subunit PrcA.
Appears to be functional by complementation study.
Appears to be non-functional by complementation study.
Uncharacterised methyltransferase Rv1498c
No longer neutralizes the toxic effect of MbcT.
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Uncharacterized SufE-like protein Rv3284
Abolishes complementation of the Rv3671c mutant. It does not exhibit autocleavage activity.
Significant decrease of protease activity.
Probable monoacyl phosphatidylinositol tetramannoside-binding protein LpqW
Loss of protease activity. Shows neither autoproteolytic nor proteolytic activity against beta-casein.
Ribosomal RNA small subunit methyltransferase H
Covalent link with the inhibitor MLN-273
Loss of proteasome proteolytic activity. This mutant enables optimal growth in vitro and in vivo, confers RNI resistance but is not sufficient for persistence in mice.
Removed in mature form; by autocatalysis
Proton acceptor; for dehydratase activity
Putative inactive phenolphthiocerol synthesis polyketide synthase type I Pks1
Uncharacterized PPE family protein PPE12
3-fold decrease in catalytic activity, and 2-fold decrease in substrate affinity.
4-fold decrease in catalytic activity with Mg(2+) as cofactor. 3-fold increase in catalytic activity with Zn(2+) as cofactor. Shifts the pH for optimal activity to 8.5.
Nearly no effect on catalytic activity with Mg(2+) as cofactor. 10-fold increase in catalytic activity with Zn(2+) as cofactor.
Bifunctional aminodeoxychorismate lyase / D-amino acid transaminase
Putative thiosulfate sulfurtransferase SseB
Transcriptional regulatory protein PdtaR
Affects protein stability. Decreases affinity for DNA and dimerization capacity.
Affects protein stability. Decreases affinity for DNA. Does not affect dimerization.
Decreases affinity for DNA. Does not affect dimerization.
Moderate impact on protein stability. Does not affect DNA binding.
Fully oxidized recombinant protein tagged at both termini.
Fully reduced recombinant protein tagged at both termini.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-59 and S-62.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-59 and S-68.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-62 and S-68.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-59; S-62 and S-68.
Adaptive response protein AidB N-terminal
Probable acetyl-CoA acetyltransferase, propeptide removed
Acetyl-CoA dehydrogenase-like C-terminal
Acyl-CoA dehydrogenase N-terminal bacteria
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0145
Could provide a fine-tuning to achieve optimal pKa matching between active site and substrate
Putative 3-methyladenine DNA glycosylase
Exopolysaccharide phosphotransferase CpsY
Tuberculostearic acid methyltransferase UfaA1
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Uncharacterized N-acetyltransferase Rv2669
Reversibly protonated during proton transport
Putative 8-amino-7-oxononanoate synthase 2
Pyridoxal 5'-phosphate synthase subunit PdxT
1200-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
1400-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
1700-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
2-oxoglutarate oxidoreductase subunit KorA
Uncharacterized PPE family protein PPE44
Putative diacyglycerol O-acyltransferase Rv1425
Peptidoglycan recognition protein family
Cyclopropane mycolic acid synthase MmaA2
TVP38/TMEM64 family membrane protein Rv1491c
Phosphate-specific transport system accessory protein PhoU homolog 2
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Does not show any significant binding to either DNA or RNA and lacks m2G966 methyltransferase activity.
Does not show any significant binding to either DNA or RNA in vitro and lacks m2G966 methyltransferase activity in vivo; when associated with A-12 and A-13.
Does not show any significant binding to either DNA or RNA in vitro and lacks m2G966 methyltransferase activity in vivo; when associated with A-3 and A-12.
Does not show any significant binding to either DNA or RNA in vitro and lacks m2G966 methyltransferase activity in vivo; when associated with A-3 and A-13.
Shows reduced nuclear localization; when associated with A-171 and A-177.
Shows reduced nuclear localization; when associated with A-171 and A-183.
Shows reduced nuclear localization; when associated with A-177 and A-183.
Uncharacterized HTH-type transcriptional regulator Rv0043c
Glyceraldehyde-3-phosphate dehydrogenase
Same dehydrogenase activity compared to the wild-type.
ATP-dependent Clp protease proteolytic subunit 1
Medium/long-chain-fatty-acid--[acyl-carrier-protein] ligase MbtM
Important for beta-aspartyl-AMP intermediate formation
Putative asparagine synthetase [glutamine-hydrolyzing]
Cyclic pyranopterin monophosphate synthase 3
12 X 8 AA approximate repeats of A-P-A-D-L-A-P-P
Ribosomal RNA small subunit methyltransferase A
Probable ribonucleotide transport ATP-binding protein mkl
Glutamyl-tRNA(Gln) amidotransferase subunit A
Bifunctional purine biosynthesis protein PurH
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase epsilon subunit
ATP-dependent 6-phosphofructokinase isozyme 2
Protein no longer targeted to encapsulin nanocompartments.
Fluoroquinolones export permease protein Rv2687c
Putative flavin-containing monoamine oxidase AofH
Copper-sensing transcriptional repressor CsoR
Decrease in ability to dissociate from DNA in the presence of copper.
Loss of ability to bind copper; loss of ability to dissociate from DNA in the presence of copper.
Loss of ability to bind to DNA; when associated with A-15 and A-36.
Loss of ability to bind to DNA; when associated with A-15 and A-52.
Loss of ability to bind to DNA; when associated with A-36 and A-52.
Reduces FtsZ polymerization and GTP hydrolysis. Does not affect the structure or the stability of the unpolymerized FtsZ.
Lack of autophosphorylation. Decreases affinity for FhaB.
Reduces activity and autophosphorylation. Decreases interaction with GarA.
Uncharacterized PE-PGRS family protein PE_PGRS10
Medium/long-chain-fatty-acid--CoA ligase FadD6
700-fold decrease in the rate of the first half-reaction using OPS. Affects neither the rate of the first half-reaction using OAS nor the rate of the second half-reaction using sulfide or CysO-COSH.
Decreased lifetime of the alpha-aminoacrylate reaction intermediate, increased susceptibility to oxidation by oxidative agents such as hydrogen peroxide, and partial loss of selectivity towards CysO-COSH as sulfur donor.
Alpha-(1->6)-mannopyranosyltransferase A
DNA-binding and helicase activity, interacts with RecC
Nuclease activity, interacts with RecD and RecA
N-acetylmuramoyl-L-alanine amidase Rv3717
Alpha-(1->6)-mannopyranosyltransferase Rv1459c
Potassium-transporting ATPase ATP-binding subunit
Terminal beta-(1->2)-arabinofuranosyltransferase
Putative peptidoglycan hydrolase Rv2525c
Putative acetolactate synthase small subunit
Decreases structure stability of OmpA-like domain.
Increases conformational stability of OmpA-like domain. Does not alter peptidoglycan-binding.
Loss of peptidoglycan-binding; in association with A236.
Required for protein translocation to the outer membrane
3'-phosphoadenosine 5'-phosphate phosphatase
Less than 2% of wild-type IMPase and FBPase activities.
Less than 3% of wild-type IMPase and FBPase activities.
No effect on IMPase and FBPase activities. Much less sensitive to inhibition by Li(+).
[Acyl-carrier-protein] phosphodiesterase PptH
Endoribonuclease L-PSP/chorismate mutase-like
It promotes stronger allosteric interactions.
Lower catalytic efficiency. A perturbation of the allosteric interactions is observed when NAD is used as substrate.
Possesses 30% of the activity compared to the wild-type enzyme. While mutant affects the catalytic efficiency, it does not alter the binding affinity for ATP and poly(P). It causes a decrease in the affinity for NAD and alters the allosteric interactions mediated by the dinucleotide, both in the presence of poly(P) and ATP.
Mycocerosic acid synthase-like polyketide synthase
ESX-1 secretion-associated protein EspG1
3-methyl-2-oxobutanoate dehydrogenase subunit alpha
Uncharacterized ABC transporter permease Rv0072
Probable glycerol-3-phosphate dehydrogenase 2 [NAD(P)+]
Type II NADH:quinone oxidoreductase NdhA
Almost completely abolishes phosphorylation by PknH.
Uncharacterized HTH-type transcriptional regulator Rv0681
Bifunctional oligoribonuclease and PAP phosphatase NrnA
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Polymerase/histidinol phosphatase N-terminal
Uncharacterized PPE family protein PPE45
Uncharacterized PE-PGRS family protein PE_PGRS20
Considerably reduced interaction with SigE. Complete loss of interaction; when associated with A-70.
Considerably reduced interaction with SigE. Complete loss of interaction; when associated with A-73.
Loss of phosphorylation, no protein degradation.
Probable dolichyl-phosphate-mannose--protein mannosyltransferase
Putative diacyglycerol O-acyltransferase Rv3740c
GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase
Probable polyglutamine synthesis accessory protein Rv0574c
Uncharacterized NTE family protein Rv1063c
Probable low-affinity inorganic phosphate transporter
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase beta6 subunit
13% supercoiling, 10% decatenation, 42% DNA cleavage in presence of norfloxacin.
15% supercoiling, 19% decatenation, 143% DNA cleavage in presence of norfloxacin.
17-fold increased resistance to fluoroquinolones, slightly increased DNA cleavage in absence of drugs.
18% supercoiling, 11% decatenation, 131% DNA cleavage in presence of norfloxacin.
37% supercoiling, 54% decatenation, 126% DNA cleavage in presence of norfloxacin.
<3% supercoiling, 18% decatenation, 75% DNA cleavage in presence of norfloxacin.
Increased resistance to aminopyrazinamides, however genome not sequenced.
Increased resistance to pyrrolamides and novobiocin, however genome not sequenced.
Increased susceptibility to fluoroquinolones, half supercoiling activity, no fluoroquinolone-induced DNA cleavage (makes sequence more like E.coli).
Does not affect formation of the PE25/PPE41 dimer, but abolishes EspG5 binding to PE25/PPE41.
Does not disrupt the interaction with EspG5.
Probable transcriptional regulatory protein NarL
Can catalyze the hydrolysis of palmitoyl-CoA, but cannot produce cyclic polyketide products.
Methyl-branched alkylpyrone synthesis polyketide synthase-like Pks11
Probable transport accessory protein MmpS3
3-isopropylmalate dehydratase small subunit
1,4-dihydroxy-2-naphthoate octaprenyltransferase
Abolishes DNA-binding and repressor activity; when associated with A-52 and A-57.
Abolishes DNA-binding and repressor activity; when associated with A-52 and A-62.
Abolishes DNA-binding and repressor activity; when associated with A-57 and A-62.
HTH-type transcriptional repressor Rv3405c
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
S-adenosylmethionine-dependent methyltransferase Rv2258c
Uncharacterized PE-PGRS family protein PE_PGRS24
Probable ferredoxin/ferredoxin--NADP reductase
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Orn/Lys/Arg decarboxylases family 1 pyridoxal-P attachment site
Uncharacterized ABC transporter permease Rv2563
Adenosylcobinamide-GDP ribazoletransferase
Restriction system protein Mrr-like N-terminal
Probable glycerophosphodiester phosphodiesterase 2
Broad-specificity linear acyl-CoA dehydrogenase FadE5
Decreases affinity for eicosanoyl-CoA; when associated with A-447.
Increases affinity for eicosanoyl-CoA; when associated with A-447.
Putative F420H(2)-dependent quinone reductase Rv3178
Putative acetolactate synthase large subunit IlvX
4-fold decrease in affinity relative to the wild-type and able to synthesize prenyl diphosphates containing 20 and 50 carbon atoms when GPP and EE-FPP are used as the allylic substrates, respectively, whereas the wild-type enzyme generates product that is restricted to 15 carbon atoms with GPP as substrate and does not react with EE-FPP; when associated with G-107.
Important for determining product length
No detectable activity. 4-fold decrease in affinity relative to the wild-type and able to synthesize prenyl diphosphates containing 20 and 50 carbon atoms when GPP and EE-FPP are used as the allylic substrates, respectively, whereas the wild-type enzyme generates product that is restricted to 15 carbon atoms with GPP as substrate and does not react with EE-FPP; when associated with A-84.
Resistant to 3bMP1 compound. Defective in uptake of propionamide, glucose and glycerol, and is unable to grow on them as the sole carbon sources, but grows normally on hexanamide.
Resistant to thio-disaccharide T-6, but not to other thio-functionalized carbohydrate derivatives, such as aminothiadiazole T-23 or thiazoline T-26. Sensitive similarly to wild-type to antitubercular drugs including isoniazid, ethambutol, streptomycin, rifampicin and thiolactomycin.
Resistant to thio-disaccharide T-6, but not to other thio-functionalized carbohydrate derivatives, such as aminothiadiazole T-23 or thiazoline T-26. Sensitive similarly to wild-type to antitubercular drugs including isoniazid, ethambutol, streptomycin, rifampicin and thiolactomycin. Reduced uptake of radiolabeled T-6 after its 48-hour incubation with the cell culture, but not after its shorter incubation time. Significantly slower growth compared to the wild-type on minimal medium supplemented with maltose or lactose as the sole carbon and energy sources at both neutral pH and acidic pH 5.7. Growth is promoted in acidic minimal medium by supplementation of glycerol or glucose compared to the wild-type. No effect on growth in rich medium.
Resistant to trehalose analog 6-azido trehalose, but not to 2-azido trehalose. Normal growth on trehalose as the sole carbon source.
Multidrug efflux ATP-binding/permease protein Rv0194
20-fold decrease in the rate of INH-NAD adduct formation. Exhibits significantly reduced affinity for INH (KM is increased by 43-fold).
Exhibits 1.7-fold decreased catalytic efficiency for the activation of INH.
Exhibits 2-fold increased affinity for INH.
Exhibits 8-fold increased catalytic efficiency for the activation of INH (INH-NAD formation). Possesses an enlarged substrate access channel.
Nearly no effect on the kinetic parameters for the activation of INH.
Nearly no effect on the kinetic parameters for the catalase and peroxidase activity. Activates INH and mediates InhA inactivation as efficiently as wild-type.
Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255); alternate
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-107)
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107); alternate
Proton donor/acceptor; for phosphatase activity
D-glycero-alpha-D-manno-heptose 7-phosphate kinase
Probable diacyglycerol O-acyltransferase tgs3
Uncharacterized PPE family protein PPE24
Important for bacterial uptake by host macrophages
Remains cell wall associated, no difference in gel migration, upon overexpression in M.smegmatis.
Transcription antitermination protein NusB
The measured mass is that of a dimer, plus 2 cadmium ions.
Sec-independent protein translocase protein TatB
Molybdopterin synthase catalytic subunit 2
Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
Completely abolishes enzymatic activity.
Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.
Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
Uncharacterized ABC transporter permease protein Rv0987
Important for substrate specificity; cannot use PPi as phosphoryl donor
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase
DNA-directed RNA polymerase subunit omega
Causes a probable loss of enzymatic activity.
Phthioceranic/hydroxyphthioceranic acid synthase
Completely abolishes the interaction with the aliphatic tail of the substrate lysine.
Induces autoacetylation of the new lysine in the absence of cAMP.
Substantially decreases the interaction with the aliphatic tail of the substrate lysine.
Substantially decreases the interaction with the aliphatic tail of the substrate lysine. Markedly reduced activity, with an altered pH-rate profile and abolishes direct interactions with R-184.
Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol mannoside mannosyltransferase
Putative diacyglycerol O-acyltransferase Rv3371
Probable doxorubicin resistance ABC transporter permease protein DrrC
11-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Does not affect nitrosylation. 80% decrease in phosphatase activity.
Lack of nitrosylation. Does not affect phosphatase activity.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. 36-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. Reduces its effect on phagolysosome fusion in infected macrophages. Does not affect nitrosylation.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. Reduces its effect on phagolysosome fusion in infected macrophages. Still binds V-ATPase subunit H. 380-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Loss of phosphatase activity; when associated with E-128.
Loss of phosphatase activity; when associated with E-129.
Low molecular weight protein-tyrosine phosphatase A
Mutant can be phosphorylated. Lack of phosphorylation and loss of phosphatase activity; when associated with A-128.
Mutant can be phosphorylated. Lack of phosphorylation and loss of phosphatase activity; when associated with A-129.
No longer binds to ubiquitin. Abolishes the Jnk- and p38-dependent production of TNF and IL-1-beta in macrophages. Also abolishes the PtpA-mediated inhibition of phagosome acidification.
Retains phosphatase activity but does not bind V-ATPase subunit H. Fails to inhibit V-ATPase trafficking to phagosomes and shows impaired intracellular survival.
Slight decrease in phosphatase activity.
Strong decrease in phosphatase activity.
Uncharacterized glycosyltransferase Rv1524
3-ketosteroid-9-alpha-monooxygenase, ferredoxin reductase component
Putative phosphoribosyl transferase Rv0571c
Loss of catalytic activity but still able to mediate uptake of glucose and phosphocholine; when associated with N-353.
Loss of catalytic activity but still able to mediate uptake of glucose and phosphocholine; when associated with N-481.
Lack of phosphorylation by PknD. Reduced oprA induction in response to osmotic stress.
Completely abolishes the diaminopimelate epimerase activity.
Could be important to modulate the pK values of the two catalytic cysteine residues
Strongly reduces the diaminopimelate epimerase activity.
Putative bifunctional exonuclease/endonuclease protein Rv2191
Uncharacterized methyltransferase Rv0089
Abolishes interaction with host ubiquitin, impairs host xenophagy and increases bacterial loads in mice with enhanced inflammatory responses.
Important for interaction with host ubiquitin
Reduces interaction with host ubiquitin.
Reduces interaction with host ubiquitin. Shows enhanced viability, reduced p62 aggregation and reduced colocalization with LC3 and LAMP1 in BMDMs.
Uncharacterized methyltransferase Rv3342
Acetolactate synthase large subunit IlvB1
200000-fold decrease in catalytic efficiency with cefamandole as substrate.
Functions as a gatekeeper residue that regulates substrate accessibility to the enzyme active site
Increases nucleophilicity of active site Ser
Loss of catalytic activity with cefamandole as substrate.
No longer exported via the Tat-system. Loss of cell resistance to beta-lactam antibiotics.
Only minor impairment of catalytic activity with both nitrocefin and ampicillin. Still inhibited by clavulanate and carbapenems.
Significant increase in ampicillin resistance. 2-fold and 3-fold increase in catalytic efficiency with ampicillin and nitrocefin as substrate, respectively, mainly due to an increase in substrate affinity.
Significant reduction of catalytic activity for both nitrocefin and ampicillin. Leads to in vitro clavulanate resistance and decreased susceptibility to carbapenem inhibitors, but is still susceptible to ampicillin-clavulanate in vivo.
Significant reduction of catalytic activity for both nitrocefin and ampicillin. Only minor impairment of the inactivation by clavulanate. Larger increase in resistance to carbapenems.
Meromycolate extension acyl carrier protein
Bifunctional lipase/adenylate cyclase LipJ
Does not substantially affect adenylate cyclase activity but increases activity with GTP.
Does not substantially affect adenylate cyclase activity.
Substantially affects adenylate cyclase activity.
Precorrin-6Y C(5,15)-methyltransferase [decarboxylating]
Uncharacterized NTE family protein Rv2565
RNA polymerase-binding transcription factor CarD
Weakens interaction with RpoB, decreases growth rate and virulence, and increases rifampicin sensitivity. Abolishes interaction with RpoB; when associated with E-25.
Weakens interaction with RpoB. Abolishes interaction with RpoB; when associated with E-47.
Cleavage (non-hydrolytic); by autocatalysis
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Phosphate transport system permease protein PstA 1
Does not block ESX-1-mediated secretion during ATP depletion. Lack of ATP-binding.
Uncharacterized HTH-type transcriptional regulator Rv1556
Fluoroquinolones export permease protein Rv2686c
3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase alpha subunit
Malto-oligosyltrehalose trehalohydrolase
Cyclic pyranopterin monophosphate synthase 1
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Uncharacterized PPE family protein PPE20
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Uncharacterized membrane protein Rv1735c
Uncharacterized MFS-type transporter Rv1250
Sec-independent protein translocase protein TatC
Decreases affinity for NaHCO(3) 3-fold, for CTP 1.8-fold and for GTP 3.8-fold.
Potassium-transporting ATPase potassium-binding subunit
10-fold decrease in enzymatic activity with NAM as substrate, and 21-fold decrease in enzymatic activity with PZA as substrate. The mutant has a low metal ion content.
100-fold decrease in enzymatic activity with NAM or PZA as substrate. The mutant has a low metal ion content.
112-fold decrease in enzymatic activity with NAM as substrate, and 164-fold decrease in enzymatic activity with PZA as substrate. The mutant has no detectable iron and manganese.
2.4-fold decrease in enzymatic activity with NAM or PZA as substrate. No effect on metal ion content.
3000-fold decrease in enzymatic activity with NAM as substrate, and 410-fold decrease in enzymatic activity with PZA as substrate. The mutant has no detectable iron and manganese.
5-fold decrease in enzymatic activity with NAM as substrate, and 3-fold decrease in enzymatic activity with PZA as substrate. No effect on metal ion content.
Loss of enzymatic activity. No effect on metal ion content.
Does not bind 4Fe-4S cluster, binds DNA.
For fully alkylated recombinant protein tagged at both termini.
Loss of 4Fe-4S cluster, loss of DNA-binding.
Loss of DNA-binding, still binds 4Fe-4S cluster.
Cholesterol ring-cleaving hydrolase IpdB subunit
When expressed from this site no longer acts as a TLR2 agonist, suggests active protein is acylated. No glycosylated form detected.
Omega-hydroxy-beta-dihydromenaquinone-9 sulfotransferase Stf3
Loss of phosphorylation by PknG/PknK/PknJ.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
40% of the wild-type enzyme activity at pH 7.5 and 27% of the wild-type enzyme activity at pH 4.
Less than 1% of the wild-type enzyme activity.
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
Putative RNA-guided DNA endonuclease Rv2885c
Medium/long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligase
ESX-5 secretion-associated protein EspG5
Markedly increases peptidolytic activity. Disappearance of the apparent obstruction in alpha rings. Designated open-gate mutant.
Putative thiosulfate sulfurtransferase SseA
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv1729c
3-methyl-2-oxobutanoate hydroxymethyltransferase
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA
Gly-cisPro motif, important for rejection of L-amino acids
Uncharacterized PE-PGRS family protein PE_PGRS34
HTH-type transcriptional regulator Rv0792c
Does not affect activity and regulation by metal ions.
No change in activity. Lack of regulation by metal ions.
Uncharacterized PPE family protein PPE54
Uncharacterized HTH-type transcriptional regulator Rv0081
Expressed in M.bovis, lipidated and glycosylated.
Putative phthiocerol dimycocerosate transporter LppX
Required for sulfate transport in E.coli
Holliday junction branch migration complex subunit RuvB
Probable 1,4-alpha-glucan branching enzyme Rv3031
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Decreases resistance to ethidium bromide, tested in M.smegmatis.
Probable triacylglyceride transporter Rv1410c
Susceptible to ethidium bromide, tested in M.smegmatis.
Phenolphthiocerol/phthiocerol polyketide synthase subunit E
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
2-fold increase in bis(pNPP) hydrolysis. 4-fold decrease in pNPP hydrolysis. 5-fold decrease in 2',3'-cAMP hydrolysis.
25% decrease in bis(pNPP) hydrolysis. 70% decrease in cAMP hydrolysis. Decreases interaction with the cell wall.
40% decrease in bis(pNPP) hydrolysis. 80% decrease in 3',5'-cAMP and 2',3'-cAMP hydrolysis.
40% decrease in bis(pNPP) hydrolysis. Strong decrease in 3',5'-cAMP and 2',3'-cAMP hydrolysis. Abolishes pNPP hydrolysis. Decreases interaction with the cell wall.
50% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Decreases interaction with the cell wall.
50% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Strong decrease in interaction with the cell wall.
75% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Decreases catalytic efficiency and shows lower affinity for both linear and cyclic substrates. Almost completely abrogates interaction with the cell wall in vitro.
8-fold decrease in phosphorylation. Affects localization to the cell wall.
80% decrease in hydrolysis of 3',5'-cAMP and bis(pNPP). 40% decrease in 2',3'-cAMP hydrolysis. Strong decrease in interaction with the cell wall.
90% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Strong decrease in interaction with the cell wall.
Does not affect localization to the cell wall.
Loss of bis(pNPP) and 3',5'-cAMP hydrolysis. Strong decrease in 2',3'-cAMP hydrolysis. Slight decrease in interaction with the cell wall.
cAMP/cGMP dual specificity phosphodiesterase Rv0805
Putative conjugal transfer protein Rv3659c
Inosine/uridine-preferring nucleoside hydrolase
Probable cation efflux system protein Rv2025c
Uncharacterized tRNA/rRNA methyltransferase Rv3579c
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
2,4-diaminopentanoate dehydrogenase C-terminal
Cells unable to export EsxB, missing transmembrane domain.
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Putative diacyglycerol O-acyltransferase Rv0895
Probable transcriptional regulatory protein Rv2603c
3-oxoacyl-[acyl-carrier-protein] synthase 2
Decreases phosphorylation. Lack of phosphorylation; when associated with A-313.
Decreases phosphorylation. Lack of phosphorylation; when associated with A-315.
Phosphomimetic mutant. Loss of acid-fast staining and reduced pathogenicity; when associated with D-313.
Phosphomimetic mutant. Loss of acid-fast staining and reduced pathogenicity; when associated with D-315.
Uncharacterized RNA pseudouridine synthase Rv1711
Decreased apparent weight in M.smegmatis, a portion of the protein is protected from extracellular trypsin.
CobB/CobQ-like glutamine amidotransferase
Activator of Hsp90 ATPase homologue 1-like C-terminal
Lack of protease activity. Has hyperactivated ESX-1 secretion during macrophage infection, resulting in increased innate immune signaling. Secretes full-length EspB.
Sodium/calcium exchanger membrane region
Alpha-pyrone synthesis polyketide synthase-like Pks18
Cannot be recovered in the soluble form.
Completely abolishes the polyketide synthase activity with lauroyl-CoA and palmitoyl-CoA.
Efficiently catalyzed the synthesis of the triketide pyrone of the C6 starter unit and shows weak polyketide synthase activity with the C12 starter molecule.
Important for broad specificity for aliphatic long-chain acyl-CoA starter units
Increase in the synthesis of the tetraketide products.
No significant change in polyketide synthase activity.
Retained reasonable polyketide synthase activity with lauroyl-CoA but cannot use palmitoyl-CoA.
Unable to synthesize any polyketide products.
Uncharacterized PPE family protein PPE62
Phosphoribosylglycinamide formyltransferase
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase beta5 subunit
N6 adenine-specific DNA methyltransferase N-terminal
Altered association with RAC components.
Bifunctional (p)ppGpp synthase/hydrolase RelA
Succinate--CoA ligase [ADP-forming] subunit alpha
CDP-diacylglycerol--serine O-phosphatidyltransferase
Partial loss of phosphorylation. Decrease in activity.
Glucose-6-phosphate dehydrogenase assembly protein OpcA C-terminal
Glucose-6-phosphate dehydrogenase assembly protein OpcA N-terminal
Plays an orienting role for the imidazole group of His-298
ATP-dependent Clp protease ATP-binding subunit ClpC1
Probable diacyglycerol O-acyltransferase tgs2
2-oxoglutarate oxidoreductase subunit KorB
Probable L-lysine-epsilon aminotransferase
2-heptyl-1-hydroxyquinolin-4(1H)-one methyltransferase
6.7-fold increase in catalytic efficiency with componud 14 as substrate.
Crossover junction endodeoxyribonuclease RuvC
Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
Trehalose import ATP-binding protein SugC
tRNA N6-adenosine threonylcarbamoyltransferase
Galactan 5-O-arabinofuranosyltransferase
TVP38/TMEM64 family membrane protein Rv0625c
Uncharacterized PPE family protein PPE32
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
Uncharacterized PPE family protein PPE21
Uncharacterized Na(+)/H(+) exchanger Rv2287
Probable transposase IS891/IS1136/IS1341
Vitamin B12-dependent ribonucleoside-diphosphate reductase
Helix-hairpin-helix DNA-binding motif class 1
Decreases affinity for ATP and autophosphorylation efficiency.
Decreases affinity for ATP and autophosphorylation efficiency. Decreases interaction with PtpA.
Lack of autophosphorylation. Decreases interaction with PtpA. Cannot phosphorylate and activate PtpA.
Significant decrease in ATP binding affinity and autophosphorylation efficiency. Decreases interaction with PtpA.
Alpha-D-phosphohexomutase alpha/beta/alpha
Probable chromosome-partitioning protein ParB
Acetolactate synthase large subunit IlvG
Uncharacterized PPE family protein PPE22
Lack of activity. Confers resistance to TP053.
Completely disrupts protein function, as shown by the dramatic decrease of RNA levels for the moa1 locus.
Has no deleterious effect on the transcription of the moa1 locus.
Shows reduced transcription of the moa1 locus.
Shows significantly reduced transcription of the moa1 locus.
Transcriptional regulatory protein MoaR1
Phenolphthiocerol/phthiocerol polyketide synthase subunit B
ADP-dependent (S)-NAD(P)H-hydrate dehydratase
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Abolishes diacylglycerol acyltransferase activity.
Diacylglycerol acyltransferase/mycolyltransferase Ag85A
Ribonucleoside-diphosphate reductase subunit beta nrdF1
Nicotinate phosphoribosyltransferase pncB1
Uncharacterized glycosyl hydrolase Rv3401
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Leads to decreased interaction with PpsE, but does not decrease MmpL7 activity in vivo.
Phthiocerol dimycocerosate exporter MmpL7
Shows less than 0.1% of wild-type activity with peptides substrates.
Shows less than 1% of wild-type activity.
Shows less than 1% of wild-type activity. Does not affect macrophage apoptosis.
Shows less than 10% of the wild-type activity towards phosphotyrosine peptides, but remains unaltered towards pNPP and phosphoserine/threonine peptides.
Shows new activity for di-phosphorylated substrates PI(3,4)P2, PI(4,5)P2 and PI(3,4,5)P3.
Triple specificity protein phosphatase PtpB
Probable fatty acyl-CoA transferase Rv3272
Malonyl CoA-acyl carrier protein transacylase
Nearly abolishes pupylation and dramatically stabilizes this proteasome substrate in wild-type mycobacteria.
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Favors the presence of monomers in solution.
Results in a conformational transition from dimer to monomer.
Decreased ConA binding; altered protein processing yields 21 and 16 kDa forms in M.smegmatis.
Decreased ConA binding; altered protein processing yields 21 and 17 kDa forms in M.smegmatis.
No ConA binding; altered protein processing yields 16 kDa soluble form starting on residue 40 in M.smegmatis. Protein poorly released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Protein not exported from cytoplasm, not released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Protein not released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Uncharacterized HTH-type transcriptional regulator Rv1830
Medium/long-chain-fatty-acid--[acyl-carrier-protein] ligase FadD10
Putative HTH-type transcriptional regulator Rv1287
Electron transfer flavoprotein subunit alpha
Phosphatidylinositol mannoside acyltransferase
Considerable loss of chaperone activity.
Retains chaperone activity; when associated with A-27, A-50 and A-53.
Retains chaperone activity; when associated with A-27, A-50 and A-59.
Retains chaperone activity; when associated with A-27, A-53 and A-59.
Retains chaperone activity; when associated with A-50, A-53 and A-59.
Leads to the production of longer chain product, specifically (E,E)-farnesyl diphosphate (FPP), in addition to GPP.
Acetyl- and succinyl-CoA transferase Rv0802c
It is catalytically catastrophic, but strongly activated by AroG.
The catalytic efficiency and the affinity are 5 and 3-fold lower than the wild-type. The activation by AroG is 10-fold lower than the wild-type.
The catalytic efficiency and the affinity are higher than the wild-type. The activation by AroG is 20-fold lower than the wild-type.
The catalytic efficiency and the affinity are identical to the wild-type. The activation by AroG is 10-fold lower than the wild type.
The catalytic efficiency and the affinity are slightly modified. The activation by AroG is 2-fold lower than the wild-type.
Probable inositol 1-monophosphatase ImpA
Uncharacterized HTH-type transcriptional regulator Rv0023
Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase
Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
Does not show activity with small, medium or long acyl chains.
Long-chain-fatty-acid--CoA ligase FadD13
No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
Reduction of binding affinity for fatty acids.
Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.
Uncharacterized PPE family protein PPE69
Tetrapyrrole biosynthesis uroporphyrinogen III synthase
Still interacts with EsxG, but impairs interaction with host HGS/HRS; when associated with A-76.
Still interacts with EsxG, but impairs interaction with host HGS/HRS; when associated with A-77.
Uncharacterized PPE family protein PPE38
In S11; may confer PZA resistance; when associated with R-21.
In S11; may confer PZA resistance; when associated with V-49.
Putative diacyglycerol O-acyltransferase Rv2484c
Uncharacterized PPE family protein PPE58
Dihydrofolate synthase/folylpolyglutamate synthase
In a spontaneous PAS-resistant mutant strain.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility, confirmimg that this mutation confers high level resistance to PAS.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility; 5-fold reduction in DHFS activity relative to wild-type; loss of the ability to glutaminate H2PtePAS.
In clinical isolate: Q36; PAS-resistant; complementation with wild-type folC restores PAS susceptibility.
In clinical isolates: Q274 and 501063; PAS-resistant; complementation with wild-type folC restores PAS susceptibility.
In clinical isolates: Q449 and 1314; PAS-resistant; complementation with wild-type folC restores PAS susceptibility; 7-fold reduction in DHFS activity relative to wild-type; loss of the ability to glutaminate H2PtePAS.
Reduces ATP-binding; M.tuberculosis overexpressing this protein grows normally.
Putative mycofactocin biosynthesis transcriptional regulator MftR
Uncharacterized MFS-type transporter EfpA
Putative coenzyme F420-dependent oxidoreductase Rv3520c
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
PGL/p-HBAD biosynthesis rhamnosyltransferase
100-fold decrease in phosphopantetheinylation activity.
20-fold decrease in phosphopantetheinylation activity.
500-fold decrease in phosphopantetheinylation activity.
Abolishes phosphopantetheinylation activity.
Confers high-level resistance to compound 8918.
Schiff-base intermediate with acetaldehyde
Absence of EspD secretion. Decreases EspA levels.
Absence of EsxA secretion. Decreases EspD stability.
Decreased EspD secretion and small reduction in cellular EspA levels.
3-methyl-2-oxobutanoate dehydrogenase subunit beta
Abolishes homodimerization and strongly decreases enzyme activitywhen associated with E-296 and R-363.
Almost complete loss of enzyme activity.
Promotes formation of a domain-swapped dimer. Abolishes homodimerization and strongly decreases enzyme activity; when associated with E-296 and C-365.
Strongly decreased enzyme activity. Abolishes homodimerization and strongly decreases enzyme activity; when associated with R-363 and C-365.
Phenolphthiocerol/phthiocerol polyketide synthase subunit D
Not absolutely required for RNase activity
Significantly decreased beta-lactamase and RNase activity.
Putative acetolactate synthase large subunit IlvB2
Probable copper-exporting P-type ATPase V
Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol pentamannoside mannosyltransferase
Probable cation-transporting P-type ATPase J
NIF system FeS cluster assembly NifU N-terminal
Probable glycerophosphodiester phosphodiesterase 1
Putative decaprenylphosphoryl-5-phosphoribose phosphatase Rv3807c
80% loss of esterase and beta-lactamase activities.
Almost no change in esterase and beta-lactamase activities.
Loss of esterase and beta-lactamase activities.
Uncharacterized ABC transporter ATP-binding protein Rv0986
Peptidase S9 prolyl oligopeptidase catalytic
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
Probable carboxylic ester hydrolase LipM
Uncharacterized PPE family protein PPE14
Putative short-chain type dehydrogenase/reductase Rv0148
Forms dimers independent of the oxidizing nature of the environment, but cannot interact with SigH and cannot rescue the growth defects of the deletion mutant; when associated with A-5.
Forms dimers independent of the oxidizing nature of the environment, but cannot interact with SigH and cannot rescue the growth defects of the deletion mutant; when associated with A-9.
Uncharacterized PPE family protein PPE36
ER-bound oxygenase mpaB/mpaB'/Rubber oxygenase catalytic
Abolishes host cell invasion and survival in host macrophages.
Putative uncharacterized protein Rv0095c
Decrease in heme binding. Strong decrease in heme binding; when associated with A-64.
Decrease in heme binding. Strong decrease in heme binding; when associated with A-90.
Important in orienting the L-aspartate substrate
Uncharacterized PE-PGRS family protein PE_PGRS46
Uncharacterized HTH-type transcriptional regulator Rv2250c
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Siderophore export accessory protein MmpS5
Severely affects the affinity toward GTP and causes a significant decrease in enzyme activity.
Signal recognition particle receptor FtsY
Probable transcriptional regulatory protein TcrX
Loss of fatty acyl dehydrogenase activity.
Pyruvate phosphate dikinase AMP/ATP-binding
Abolishes phosphorylation by PknB. Strong decrease in affinity for MviN.
Decreases ability to bind to and regulate the activities of Gdh and GltB, but does not affect ability to inhibit Kgd.
Does not affect phosphorylation by PknG.
Peptidase S33 tripeptidyl aminopeptidase-like C-terminal
Probable transcriptional regulator WhiB6
Phenolphthiocerol/phthiocerol polyketide synthase subunit A
Uncharacterized metal-dependent hydrolase TatD
Chromosomal replication initiator protein DnaA
Phosphate import ATP-binding protein PstB 1
Ribosomal RNA small subunit methyltransferase E
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
23% decrease of nickel uptake. Decreases or abolishes resistance against several antibiotics.
65% decrease of nickel uptake. Decreases or abolishes resistance against several antibiotics.
66% decrease of nickel uptake. Decreases or abolishes resistance against several antibiotics.
92% decrease of nickel uptake. Decreases or abolishes resistance against several antibiotics.
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Phosphoribosylformylglycinamidine synthase subunit PurQ
Abolishes EsxB-EsxA heterodimer interaction with EccCb1 and EspA, maintains interaction with EccA2 and EccE2.
Abolishes EsxB-EsxA heterodimer interaction with EccCb1, maintains interaction with EspA, EccA2 and EccE2.
Abolishes EsxB-EsxA heterodimer interaction with EccCb1, weaker interaction with EspA, maintains interaction with EccA2 and EccE2.
Abolishes interaction with EccCb1, but not with EsxA.
Abolishes interaction with EccCb1, but not with EsxA. No change in stability, but loss of secretion.
Required for ESAT-6/CFP-10 complex to bind to host macrophage and monocytes
Long-chain-fatty-acid--AMP ligase FadD28
No activity with lauric acid (C12 fatty acid) as substrate. Instead, it catalyzes activation of acetic acid (C2 fatty acid) as acetyl-AMP.
Shows substantial decrease (69%) in the activity with lauric acid (C12 fatty acid) as substrate but shows a 4-fold higher activity with the long chain fatty acyl capric acid (C10 fatty acid) as substrate.
2'-acyl-2-O-sulfo-trehalose (hydroxy)phthioceranyltransferase PapA1
O-(3'-phospho-DNA)-tyrosine intermediate
Molybdenum import ATP-binding protein ModC
Complete loss of acetyl-coenzyme A synthetase activity.
No autophosphorylation, no phosphate transfer to DevR (DosR).
Oxygen sensor histidine kinase response regulator DosT
Deoxyuridine 5'-triphosphate nucleotidohydrolase
3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase beta subunit
Loss of activity. Still forms heterotetramers.
Retains 2-4% of activity. Still forms heterotetramers.
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B
N(G),N(G)-dimethylarginine dimethylaminohydrolase
Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase
HTH-type transcriptional regulator Rv0324
Hydrophilic compounds import ATP-binding/permease protein BacA
Loss of cobalamin transport. Shows background ATPase activity.
Phosphate-specific transport system accessory protein PhoU homolog 1
Medium/long-chain-fatty-acid--CoA ligase FadD8
Carbamoyl-phosphate synthase small chain
Can rescue the moenomycin sensitivity phenotype and the aberrant short-length phenotype of the deletion mutant.
Can rescue the moenomycin sensitivity phenotype of the deletion mutant, but cannot rescue the aberrant short-length phenotype.
DNA-directed RNA polymerase subunit beta'
Probable ATP-dependent helicase DinG homolog
Cholesterol ring-cleaving hydrolase IpdA subunit
Cannot bind DNA; when associated with A-45 and A-50.
Cannot bind DNA; when associated with A-45 and A-55.
Cannot bind DNA; when associated with A-50 and A-55.
HTH-type transcriptional repressor Rv1474c
Copper-sensing transcriptional repressor RicR
Decreases activity and negatively affects cell wall formation. Attenuates virulence in a macrophage model with promoted cytokine production in host cells.
Glucose-1-phosphate thymidylyltransferase
Significant loss of phosphorylation. Lack of phosphorylation; when associated with A-54. Decreases activity and negatively affects cell wall formation.
Small decrease in phosphorylation. Lack of phosphorylation; when associated with A-12.
Dihydrolipoyllysine-residue acyltransferase component of branched-chain alpha-ketoacid dehydrogenase complex
Uncharacterized MFS-type transporter Rv2994
Uncharacterized response regulatory protein Rv3143
Probable N-succinyldiaminopimelate aminotransferase DapC
Phosphatidyl-myo-inositol mannosyltransferase
10-fold decrease in the specificity constant for thiosulfate compared to wild-type enzyme but little change in the specificity constant for the substrate OPS.
Long-chain-fatty-acid--CoA ligase FadD15
Abolishes transferase activity. Decrease in TOH production. Almost loss of iso-TOH production.
Decrease in TOH production and almost loss of iso-TOH production. Almost loss of TOH and TOH production; when associated with F-51.
Does not affect TOH production. Strong decrease in iso-TOH production.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-81 or N-82.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-81 or N-85.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-82 or N-85.
Strong decrease in TOH and iso-TOH production. Almost loss of TOH and TOH production; when associated with F-90.
Uncharacterized HTH-type transcriptional regulator Rv0377
Probable ECF RNA polymerase sigma factor SigI
40% reduction in liposome disruption. 80% reduction; when associated with 2-T--G-10.
40% reduction in liposome disruption. 80% reduction; when associated with 86-T--A-95.
40% reduction in liposome disruption. 88% reduction in liposome disruption; when associated with E-17. Reduced liposome disruption; when associated with T-38.
88% reduction in liposome disruption; when associated with 25-T-A-26.
Bacteria no longer gain access to host cytoplasm via phagosomal rupture.
Does not restore virulence in an esxA deletion strain, decreased secretion of EsxA and EsxB.
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB (PubMed:16048998). Partially inhibits host TLR2-signaling (PubMed:17486091).
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB but no complex formed (PubMed:16048998). No effect on host TLR2-signaling.
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB.
NBD-linked protein does not fluoresce at acidic pH in presence of liposomes, this residue may face inwards in membranes away from lipids.
NBD-linked protein fluoresces at acidic pH in presence of liposomes, suggests this residue faces lipids.
NBD-linked protein moderately fluoresces at acidic pH in presence of liposomes, suggests this residue is partially within membrane.
No longer inhibits host TLR2-signaling (PubMed:17486091). Does not interact with host B2M, does not decrease B2M cell surface expression, no defects in antigen presentation (PubMed:25356553).
Not required for ESAT-6/CFP-10 complex to bind to host macrophage and monocytes
Partially restores virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB (PubMed:16048998). Partially inhibits host TLR2-signaling (PubMed:17486091).
Reduced liposome disruption; when associated with 25-T-A-26.
Wild-type virulence in an esxA deletion strain.
Wild-type virulence in an esxA deletion strain; protein should not be acetylated.
Probable mycobacterial cidal antitoxin Rv3188
2-fold increase in ATPase activity (residues 72-448 only).
6-fold increase in ATPase activity (residues 106-463 only).
No ATPase activity (residues 106-480 only).
Retains 40% ATPase activity (in residues 72-480).
Retains only 20% decrease ATPase activity (in residues 72-480); probably due to loss of secondary structure.
Putative diacyglycerol O-acyltransferase Rv0221
Gamma-glutamyl-hercynylcysteine sulfoxide hydrolase
Loss of ATPase and DNA unwinding, partially inhibits DNA strand exchange.
Mycofactocin precursor peptide peptidase
N5-carboxyaminoimidazole ribonucleotide synthase
No interaction with PbpB (PBP3, FtsI). Slower growth, increased sensitivity to H(2)O(2) and to antibiotics ofloxacin and isoniazid, increased degradation of PbpB. Decreased survival in human macrophage line THP-1.
Strong decrease in phosphorylation of Wag31.
Putative pterin-4-alpha-carbinolamine dehydratase
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Uncharacterized RNA pseudouridine synthase Rv1540
Heat-inducible transcription repressor HrcA
Loss of growth, no ATPase activity. No DNA unwinding. Does not translocate along DNA.
Not essential for growth, no helicase activity. Wild-type ATPase activity, translocates along DNA. No DNA unwinding.
Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT C-terminal
Acetylserotonin O-methyltransferase dimerisation
Abolishes phosphorylation. Does not affect activity.
Mimics constitutive phosphorylation. Exhibits markedly decreased transacylation, malonyl-AcpM decarboxylation and condensing activities.
Mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III
Retains 21% of wild-type activity. Loss of activity; when associated with A-161.
Retains 69% of wild-type activity. Loss of activity; when associated with A-42 or A-46.
Retains 7% of wild-type activity. Loss of activity; when associated with A-161.
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Penicillin-binding protein transpeptidase
Uncharacterized ABC transporter ATP-binding protein Rv2326c
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Nearly abolishes DHNA-CoA synthase activity.
Reduces affinity for substrate. Nearly abolishes DHNA-CoA synthase activity.
Impairs interaction with PknB and PapA5.
Impairs interaction with PknB. Does not affect interaction with PapA5.
Does not bind DNA and does not form dimers.
Does not bind DNA, but retains the capacity to form dimers; when associated with G-32.
Does not bind DNA, but retains the capacity to form dimers; when associated with G-35.
HTH-type transcriptional regulator Rv2034
Probable S-adenosylmethionine-dependent methyltransferase Rv3030
The binding mode of azoles and some arylamines are reverted from type II to type I because of hydrophobic and steric interactions with the alanine side chain.
17.8-fold decrease in catalytic efficiency.
2.6-fold decrease in catalytic efficiency.
2.7-fold decrease in catalytic efficiency.
3.3-fold decrease in catalytic efficiency.
4.7-fold decrease in catalytic efficiency.
44.5-fold decrease in catalytic efficiency.
8.9-fold decrease in catalytic efficiency.
No large change in protein assembly or structure, decreased thermostability, 3.5-fold reduction in oxidation of Fe(2+), 20% reduction in Fe(3+) release rate.
Uncharacterized MFS-type transporter Rv1877
Increases KdpD induction at 0 and 250 uMK(+).
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase
Uncharacterized PPE family protein PPE25
DNA-binding protein HupB, propeptide removed
No change in trimethylation by human SUV39H1.
Significantly decreased trimethylation of HupB by host SUV39H1, increased bacterial survival in human macrophages, SUV39H1 no longer inhibits biofilm formation.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0726c
Putative succinate-semialdehyde dehydrogenase [NADP(+)] 2
Uncharacterized PPE family protein PPE13
Attenuates the ability to bind salicylate.
HTH-type transcriptional repressor Rv2887
Thiolase-like protein type 1 additional C-terminal
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
Probable biotin-dependent acyl-coenzyme A carboxylase beta3 subunit
Putative envelope-preserving system protein Rv2743c
2.1-fold decrease in KM for ADPR. 1940-fold decrease in kcat with ADPR as substrate.
2.3-fold decrease in KM for ADPR. 1150-fold decrease in kcat with ADPR as substrate.
2.7-fold decrease in KM for ADPR. 300-fold decrease in kcat with ADPR as substrate.
3-fold decrease in KM for ADPR. 910-fold decrease in kcat with ADPR as substrate.
Multidrug efflux system permease protein Rv1217c
Trehalose-2-sulfate acyltransferase PapA2
Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase beta1 subunit
Uncharacterized PPE family protein PPE50
About 50% succinylation in vitro, reduces specific activity 7-fold.
Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
Very low levels of succinylation in vitro, reduces specific activity 15-fold.
Decreases ATPase activity. Abolishes autophosphorylation. Can still be phosphorylated by PknD.
Does not affect ATPase activity. Abolishes autophosphorylation.
Does not affect autophosphorylation. 10-fold decrease in phosphatase activity.
Leads to the accumulation of the autophosphorylated protein. 10-fold decrease in phosphatase activity.
Strong decrease in ATPase activity. Abolishes autophosphorylation.
Trehalose transport system permease protein SugB
Uncharacterized NAD-dependent oxidoreductase Rv0687
Uncharacterized aminotransferase Rv2231c
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
NADH:ubiquinone oxidoreductase 30kDa subunit
Involved in ionization of N3 of dUMP, leading to its activation
Loss of catalytic activity since it is not able to complement an E.coli thyA deletion mutant.
Still able to complement an E.coli thyA deletion mutant.
Cytochrome bc1 complex cytochrome c subunit
(5R,7aS)-5-hydroxy-7a-methyl-1-oxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA reductase
Transposase for insertion sequence element IS1081
2-oxoglutarate dehydrogenase E1, C-terminal part
2-oxoglutarate dehydrogenase E1, N-terminal part
Multifunctional 2-oxoglutarate metabolism enzyme
Proton acceptor; for succinyltransferase activity
Dihydrodipicolinate reductase N-terminal
Glucose-methanol-choline oxidoreductase C-terminal
Glucose-methanol-choline oxidoreductase N-terminal
Uncharacterized PPE family protein PPE37
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Positions MEP for the nucleophilic attack
Decrease in acyl-CoA thioesterase activity.
Phosphate transport system permease protein PstA 2
3-ketosteroid-9-alpha-monooxygenase, oxygenase component
Uncharacterized tRNA/rRNA methyltransferase Rv0881
Shikimate dehydrogenase substrate binding N-terminal
Molybdenum transport system permease protein ModB
Flavin-dependent monooxygenase, oxygenase subunit HsaA
Shows higher efflux activity. Causes clinically relevant high-level drug resistance to pyrazinamide (PZA), isoniazid (INH) and streptomycin (SM).
Shows higher efflux activity. Causes clinically relevant low-level drug resistance to pyrazinamide (PZA), isoniazid (INH) and streptomycin (SM).
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2
Abolished formylglycine-generating enzyme activity.
Strongly reduced formylglycine-generating enzyme activity.
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1
No effect on activity. 10-fold more resistant to inhibition by Li(+).
ESX-2 secretion-associated protein EspG2
17-hydroxy-3-oxo-4-pregnene-20-carboxyl-CoA lyase
Probable diacyglycerol O-acyltransferase Tgs4
Uncharacterized ABC transporter ATP-binding protein Rv2564
L-threonine dehydratase biosynthetic IlvA
Glycosyl transferase family 28 C-terminal
Uncharacterized S-adenosylmethionine-dependent methyltransferase Rv3037c
Plays an important role in substrate specificity
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Cannot complement the growth defect of the disruption mutant. Cannot cleave GroEL2. Is unable to hydrolyze azocasein.
Small decrease in activity with azocasein as substrate. Almost abolishes cleavage of GroEL2.
Helical; Signal-anchor for type II membrane protein
Bifunctional apolipoprotein N-acyltransferase/polyprenol monophosphomannose synthase
Probable lipoprotein aminopeptidase LpqL
(7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA hydrolase
Decrease in autophosphorylation activity. Does not affect transphosphorylation activity.
Does not affect autophosphorylation and transphosphorylation activities.
Strong decrease in autophosphorylation activity. Lack of transphosphorylation activity.
Phenolphthiocerol/phthiocerol polyketide synthase subunit C
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Uncharacterized ABC transporter ATP-binding protein Rv1273c
Uncharacterized SURF1-like protein Rv2235
No phosphorylation by EnvZ kinase in vitro, does not bind DNA. Poor growth in infected macrophages and upon mouse infection. Fewer bacteria are mistargeted to lysosomes.
No phosphorylation by EnvZ kinase in vitro. Binds DNA in the absence but not presence of EnvZ and ATP. A merodiploid strain has late growth defects. May mimic the phosphorylated state.
No phosphorylation by EnvZ kinase in vitro. Binds fbpB and oriCDNA in the presence and absence of EnvZ and ATP. A merodiploid strain has late growth defects, fbpB is down-regulated. May be constitutively active.
Phosphorylated by EnvZ kinase in vitro, reverses many phenotypes of mtrB knockout.
Probable nicotinate-nucleotide adenylyltransferase
Loss of resuscitating activity on M.luteus.
Putative ATP-binding protein Rv3427c in insertion sequence
Uncharacterized PPE family protein PPE29
Putative peptide transport permease protein Rv1283c
Decrease in substrate-binding and in catalytic efficiency.
Does not affect substrate-binding, but decreases the catalytic efficiency and the Vmax.
Does not distord the binding site architecture. No change in catalytic efficiency and Vmax.
Large increase in the Km and strong decrease in catalytic efficiency. Loses the ability to self-associate in the presence of ATP and the cooperativity is nearly abolished.
Flavin-dependent monooxygenase, reductase subunit HsaB
Type 1 encapsulin shell protein, N-terminally processed
2200-fold decrease in catalytic efficiency with coenzyme A.
6600-fold decrease in catalytic efficiency with coenzyme A.
Propanal dehydrogenase (CoA-propanoylating)
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv1896c
Putative envelope-preserving system protein Rv2742c
Uncharacterized PPE family protein PPE49
p-hydroxybenzoic acid--AMP ligase FadD22
Peptide methionine sulfoxide reductase MsrA
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
Probable multidrug-efflux transporter Rv1634
2-fold decrease in glycohydrolase activity. Intermediate cytotoxicity.
Decreases glycohydrolase activity and cytotoxicity in macrophages.
Lack of glycohydrolase activity and of cytotoxicity; when associated with K-822.
Lack of glycohydrolase activity and of cytotoxicity; when associated with N-792.
Unfolded. Lack of glycohydrolase activity. Abolishes toxicity.
Loss of PA-824 and menadione reductase activity.
PGL/p-HBAD biosynthesis glycosyltransferase Rv2958c
Glycosyltransferase subfamily 4-like N-terminal
Uncharacterized AAA domain-containing protein Rv2559c
Probable trans-aconitate 2-methyltransferase
Imidazole glycerol phosphate synthase subunit HisF
Uncharacterized HTH-type transcriptional regulator Rv1255c
Acyl-ester intermediate; for acyltransferase 1 activity
Acyl-ester intermediate; for acyltransferase 2 activity
Acyl-thioester intermediate; for beta-ketoacyl synthase 1 activity
Acyl-thioester intermediate; for beta-ketoacyl synthase 2 activity
Proton acceptor; for dehydratase activity 1
Proton acceptor; for dehydratase activity 2
Proton donor; for dehydratase activity 1
Proton donor; for dehydratase activity 2
Probable molybdenum cofactor guanylyltransferase
Does not affect secretion, but inhibits disulfide bond formation. Alters mycobacterial cell wall integrity and significantly attenuates virulence.
Impairs EsxA and EsxB secretion in vitro. Modest impact on EspD stability and secretion. Attenuated in cellular models of tuberculosis infection.
Severely destabilizes EspA and blocks ESX-1 secretion in vitro, but does not impact the cytotoxicity in cellular models of infection. Can induce pro-inflammatory responses in THP-1 cells and is as virulent as wild-type during the acute phase of infection in mice.
Severely destabilizes EspA and blocks ESX-1 secretion in vitro. Attenuated in cellular models of tuberculosis infection.
Phosphatidic acid phosphatase type 2/haloperoxidase
Uncharacterized PPE family protein PPE61
Cation-transporting P-type ATPase C-terminal
HTH-type transcriptional repressor KstR2
Putative diacyglycerol O-acyltransferase Rv2285
Probable peptidyl-prolyl cis-trans isomerase B
Probable DNA polymerase III subunit delta
Putative oxidoreductase/dehydrogenase Rossmann-like
Lack of phosphorylation by PknF; when associated with A-25.
Lack of phosphorylation by PknF; when associated with A-54.
Probable methylmalonyl-CoA mutase large subunit
Nicotinate phosphoribosyltransferase pncB2
Uncharacterized HTH-type transcriptional regulator Rv3095
Long-chain-fatty-acid--AMP ligase FadD26
Uncharacterized HTH-type transcriptional regulator Rv0474
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Uncharacterized PPE family protein PPE65
Common variant in the Beijing-W clade/SCG-2 phylogenetic group.
Holliday junction branch migration complex subunit RuvA
4-hydroxyphenylalkanoate adenylyltransferase
2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1.
4-hydroxy-tetrahydrodipicolinate reductase
Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged.
Lack of phosphoglycerate mutase activity.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Uncharacterized HTH-type transcriptional regulator Rv1828
3-isopropylmalate dehydratase large subunit
Abolishes proteasome substrate degradation and protection against RNI.
Abolishes proteasome substrate degradation.
Docks into pockets in the proteasome alpha-ring
Does not dramatically affect proteasome substrate degradation.
Impairs Mpa hexamerization; when associated with A-187 and E-235.
Impairs Mpa hexamerization; when associated with E-173 and A-187.
Impairs Mpa hexamerization; when associated with E-173 and E-235.
Reduces both ATPase activity and ATP affinity. Abolishes proteasome substrate degradation and protection against RNI.
Retains ATPase and unfolding activities, yet abolishes proteasome substrate degradation and protection against RNI. Is also highly attenuated in mice.
Severely reduces ATPase activity. Abolishes proteasome substrate degradation and protection against RNI.
Severely reduces ATPase activity. Abolishes protection against RNI.
CRP-like cAMP-activated global transcriptional regulator
Putative diacyglycerol O-acyltransferase Rv1760
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
No effect on activity. Decreases Km for NADP 2-fold.
Decaprenylphosphoryl-2-keto-beta-D-erythro-pentose reductase
Phosphate import ATP-binding protein PstB 2
Lack of phosphorylation. Increases the growth of mycobacteria.
Imidazole glycerol phosphate synthase subunit HisH
Polyribonucleotide nucleotidyltransferase
Probable transport accessory protein MmpS2
Siderophore export accessory protein MmpS4
Almost loss of activity. Shows very poor binding to ATP.
Decreases Km for both ATP and dephospho-CoA. Has minimal effect on kcat.
Does not affect Km for ATP, but shows a 15-fold increase in the Km for dephospho-CoA. Decrease in kcat and strong decrease in catalytic efficiency.
Does not affect Km for ATP, but shows a 2.5-fold increase in the Km for dephospho-CoA.
Does not change either the Km or the kcat of the reaction considerably.
Increases Km for both ATP and dephospho-CoA, but increases the enzymatic turnover.
Shows a 50% increase in the Km for ATP and a 19% reduction in kcat.
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
No effect. No longer neutralizes VapC4 in full-length VapB4; when associated with G-48; R-48 or E-54.
No effect. No longer neutralizes VapC4 in full-length VapB4; when associated with low levels of G-48.
No effect. No longer neutralizes VapC4 in full-length antitoxin, decreased interaction with VapC4 (tested for G-48 only); when associated with A-64.
No effect. No longer neutralizes VapC4 in full-length antitoxin; when associated with A-64.
No longer neutralizes VapC4 in full-length VapB4; when associated with low levels of G-48.
Not required to interact with or neutralize cognate toxin VapC4
Sufficient to interact with and neutralize VapC4
2-fold decrease in kcat for 4-methylumbelliferyl heptanoate and loss of phopholipase A activity; when associated with C-132.
2-fold decrease in kcat for 4-methylumbelliferyl heptanoate and loss of phopholipase A activity; when associated with C-288.
Loss of activity for both short- and long-chain substrates.
50% reduction in oxidation activity of dithiothreitol and 60% reduction in oxidation of thiocyanate.
50% reduction in oxidation activity of dithiothreitol and thiocyanate.
In reconstituted in vitro system retains no enzyme activity.
Interchain (with C-133 in AhpD); transient
Retains about 10% activity with tert-butylhydroperoxide. In reconstituted in vitro system retains 30% enzyme activity.
Very poor oxidation activity of dithiothreitol and thiocyanate.
D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Release factor glutamine methyltransferase
Phosphate transport system permease protein PstC 1
Putative diacyglycerol O-acyltransferase Rv3087
N5-carboxyaminoimidazole ribonucleotide mutase
Transcription termination/antitermination protein NusG
Abolishes ATP-binding and diadenylate cyclase activity.
S-adenosylmethionine-dependent methyltransferase UmaA
dTDP-4-amino-4,6-dideoxyglucose formyltransferase
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit A
Uncharacterized HIT-like protein Rv1262c
Biotin-dependent acyl-coenzyme A carboxylase alpha3 subunit
Type I restriction modification DNA specificity
NAD(P) transhydrogenase alpha subunit C-terminal
Confers resistance to LPZS. Remains susceptible to IPA.
Confers resistance to Q203 and IPA. Remains susceptible to LPZS.
Cytochrome bc1 complex cytochrome b subunit
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
1500-fold decrease in catalytic activity while no effect on lipid substrate affinity.
24-fold decrease in catalytic activity while no effect on lipid substrate affinity.
Confers INH and ETH resistance. The mutant is 17 times more resistant to inhibition by the INH-NAD adduct. 5- to 6-fold decrease in affinity for NADH that results from a perturbation in the hydrogen-bonding network that stabilizes NADH binding. Nearly no effect on the velocity of the enzyme. Has no impact on the susceptibility to pyridomycin.
Confers pyridomycin resistance. Has no impact on the susceptibility to isoniazid and moxifloxacin. 14-fold decrease in NADH affinity, while no effect on catalytic activity.
Enoyl-[acyl-carrier-protein] reductase [NADH]
May act as an intermediate that passes the hydride ion from NADH to the substrate
No effect on catalytic activity. Loss of phosphorylation. Does not alter growth of M.tuberculosis.
No effect on the enzyme's catalytic ability or on its ability to bind NADH.
Severely impairs catalytic activity, as a consequence of a reduced binding affinity to NADH. These single point mutations are lethal to M.tuberculosis. These mutants fail to complement growth and mycolic acid defects of an inhA-thermosensitive M.smegmatis strain, in a similar manner to what is observed following isoniazid treatment.
Uncharacterized PPE family protein PPE28
ATP-dependent Clp protease proteolytic subunit 2
Long-chain-fatty-acid--AMP ligase FadD23
Uncharacterized HTH-type transcriptional regulator Rv2282c
Probable nitrate/nitrite transporter NarK2
No change in activity. Does not affect regulation by metal ions.
Not required for adenylyltransferase activity, required for nick joining
Fatty acid ABC transporter ATP-binding/permease protein
3 X 4 AA approximate repeats of [DA]-P-N-A
Alanine and proline-rich secreted protein Apa
Chemically or enzymatically deglycosylated protein.
Probable peptidoglycan glycosyltransferase FtsW
Phosphate transport system permease protein PstC 2
ABC transporter ATP-binding/permease protein Rv1747
Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
Strong decrease in phosphorylation. Decreases interaction with PknF.
Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
Doxorubicin resistance ABC transporter permease protein DrrB
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0146
D-inositol 3-phosphate glycosyltransferase
ATP-dependent Clp protease adapter protein ClpS
Putative membrane protein insertion efficiency factor
Putative peptide transport permease protein Rv1282c
Uncharacterized PPE family protein PPE16
Abolishes pupylation and depupylase activity.
Completely inactive mutant. The bifurcated hydrogen bond between D-270 and the ribose of NAD is replaced by a single hydrogen bond.
Biotin-dependent long chain acyl-coenzyme A carboxylase beta4 subunit
PaaX domain-containing protein, C-domain protein
Decaprenyl-phosphate N-acetylglucosaminephosphotransferase
Important in orienting the substrate; probably interacts with magnesium or manganese
Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead
Putative inactive phenolphthiocerol synthesis polyketide synthase type I Pks15
Uncharacterized GMC-type oxidoreductase Rv0492c
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
Maximal improvement in solubility; when associated with M-47 and K-69.
Maximal improvement in solubility; when associated with T-6 and K-69.
Maximal improvement in solubility; when associated with T-6 and M-47.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0281
Energy-dependent translational throttle protein EttA
Decreases affinity for Mn3 by 4-fold, no effect on hydrolysis of p-nitrophenyl phosphate.
No change in affinity for Mn3, no effect on hydrolysis of p-nitrophenyl phosphate.
Serine/threonine protein phosphatase PstP
Cyclic pyranopterin monophosphate synthase 2
Large-conductance mechanosensitive channel
Acyl-[acyl-carrier-protein] dehydrogenase MbtN
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG
Transcription termination/antitermination protein NusA
Probable cobalt/nickel-exporting P-type ATPase
Able to bind DNA, no longer induces hypoxic response.
DNA-binding transcriptional activator DevR/DosR
No longer induces expression of DevR regulon under hypoxic conditions, phosphorylated slowly and incompletely by DevS, decreased cooperative binding of target DNA.
No phosphorylation by DevS (DosS) nor by DosT.
PGL/p-HBAD biosynthesis glycosyltransferase Rv2957
6 X 5 AA approximate tandem repeats of P-[ATG]-[LG]-X-X
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0830
Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose
Phenylalanine--tRNA ligase alpha subunit
Essential for translocation to the cell surface
Diacylglycerol acyltransferase/mycolyltransferase Ag85B
Prepilin type IV endopeptidase peptidase
Confers resistance to BTZ043 and PBTZ169. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, and only slightly affects catalytic efficiency. Is only partially inhibited by PBTZ169 at high concentrations, while totally inhibited by the non-covalent inhibitor Ty38c.
Confers resistance to BTZ043 and PBTZ169. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, but reduces catalytic efficiency by 4-fold. Is only partially inhibited by PBTZ169 at high concentrations, while totally inhibited by the non-covalent inhibitor Ty38c.
Confers resistance to BTZ043 and PBTZ169. Loss of covalent modification with BTZ043. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, but reduces catalytic efficiency by 4-fold. Is only partially inhibited by PBTZ169 at high concentrations, while nearly totally inhibited by the non-covalent inhibitor Ty38c.
Decaprenylphosphoryl-beta-D-ribose oxidase
In a spontaneous TCA1-resistant mutant strain, but sensitive to BTZ.
In strain: TRC11; Ty38c-resistant mutant strain lacking Rv3406, but sensitive to moxifloxacin.
In strain: TRC12; Ty38c-resistant mutant strain lacking Rv3406, but sensitive to moxifloxacin.
Significantly less susceptible to Ty38c inhibition. 34-fold reduction in catalytic activity.
Significantly less susceptible to Ty38c inhibition. 7-fold reduction in catalytic activity.
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
Determinant for substrate specificity and/or DNA glycosylase activity
Methylphosphotriester-DNA--protein-cysteine methyltransferase
Nucleophile; methyl group acceptor from methylphosphotriester
Probable bifunctional transcriptional activator/DNA repair enzyme AlkA
Proton acceptor; for DNA glycosylase activity
Bacterial bifunctional deaminase-reductase C-terminal
Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit
2.5-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
2.7-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
3-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
Complete loss of POA binding, significantly decreased binding of tmRNA (expressed as residues 285-481).
Does not bind POA. Binds tmRNA less strongly than wild-type, binding is not disrupted by POA.
Cannot be phosphorylated. No change in activity.
Catalyzes the formation of acyl-CoA; when associated with A-383.
Catalyzes the formation of acyl-CoA; when associated with A-481.
Confers resistance to the coumarin inhibitors.
Long-chain-fatty-acid--AMP ligase FadD32
Phosphomimetic mutant. 55% decrease in activity.
No longer increases KdpD induction at 0 and 250 uMK(+); when associated with S-185 and V-197.
No longer increases KdpD induction at 0 and 250 uMK(+); when associated with T-132 and S-185.
No longer increases KdpD induction at 0 and 250 uMK(+); when associated with T-132 and V-197.
Putative diacylated glycolipid transporter LprF
Could activate a water molecule for attack at the C2 of chorismate and involved in recognition/elimination of the C4 hydroxyl
Only the chorismate mutase activity is observed.
Probable methylmalonyl-CoA mutase small subunit
Uncharacterized glycosyl hydrolase Rv2006
30% decrease in activity with pNP-octanoate and vinyl-caprylate as substrates.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. 17-18% increase in activity with VC6 or VC8 as substrates. Acquires phospholipase A activity and shows cytotoxic effects on macrophages.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. 31% and 25% decrease in activity with 6 carbon atoms vinyl esters (VC6) or 8 carbon atoms vinyl esters (VC8) as substrates. Enhances lipase activity on triglycerides. Acquires phospholipase A activity and shows cytotoxic effects on macrophages.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. Loses 99% and 80% of activity with VC6 or VC8 as substrates. Loss of lipase activity.
F420-dependent glucose-6-phosphate dehydrogenase
Decreased interaction with Wag31. Loss of interaction, reduced protection from Rip1 proteolysis; when associated with 427-H--V-429 deletion.
Decreased interaction with Wag31. Loss of interaction, reduced protection from Rip1 proteolysis; when associated with 488-Q--S-490 deletion.
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
ATP-dependent Clp protease ATP-binding subunit ClpX
Considerable effects on the heme macrocycle conformation. Mutant leads to a more planar heme conformation.
Has little effect on the heme conformation but significantly alters the environment of the heme and the affinity for azoles.
Participates in a stacking interactions with the tyrosyl of cYY
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, propeptide removed
Probable biotin-dependent acyl-coenzyme A carboxylase beta2 subunit
Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant
Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX
550-fold decrease in catalytic activity.
9000-fold decrease in catalytic activity.
Putative succinyl-diaminopimelate desuccinylase DapE
Uncharacterized GMC-type oxidoreductase Rv1279
DNA-directed RNA polymerase subunit beta
Does not affect association with TRCF, but weakens interaction with CarD.
In strain: vr11 and RJ37; rifampicin-resistant.
In strain: vr12 and SP22; rifampicin-resistant.
Peptidase M1 membrane alanine aminopeptidase
Uncharacterized HTH-type transcriptional regulator Rv0494
Putative glutamine amidotransferase Rv2859c
Lack of phosphorylation. Retains DNA-binding activity.
Incorrect processing of pre-crRNA, binds pre-crRNA.
Incorrect processing of pre-crRNA, no longer binds pre-crRNA.
Succinate dehydrogenase 2 membrane subunit SdhC
Probable CDP-diacylglycerol pyrophosphatase
Trk system potassium uptake protein TrkA
10-fold reduction in transphosphorylation activity. This mutant strain shows severely compromised growth. Loss of autophosphorylation activity and weak transphosphorylation activity; when associated with A-174.
4-fold reduction in transphosphorylation activity.
5-fold reduction in transphosphorylation activity. Loss of autophosphorylation activity and weak transphosphorylation activity; when associated with A-172.
Loss of auto- and transphosphorylation activity.
Loss of autophosphorylation activity. This mutation results in cell death.
Required for oligomerization and to complement a conditional disruption mutant
Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal
Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding
Nucleoside triphosphate pyrophosphohydrolase
Pyrophosphohydrolase activity is reduced 20-fold. It affects the magnesium binding and the protein structure.
3-phosphoshikimate 1-carboxyvinyltransferase
Uncharacterized glycosyltransferase Rv1514c
N-acetyl-gamma-glutamyl-phosphate reductase
No longer toxic, does not degrade NAD(+).
HTH-type transcriptional regulator MmpR5
In CFZ-R1; leads to increased expression of mmpL5 and partial resistance to both clofazimine and bedaquiline.
Uncharacterized PPE family protein PPE42
Required for protein stability or solubility
Potassium-transporting ATPase KdpC subunit
Mycobactin import ATP-binding/permease protein IrtB
In strain: 9106, 9181, An03, F12, F18 and TCVGH21; resistant to streptomycin.
In strain: C37; probably resistant to streptomycin.
In strain: F05; resistant to streptomycin.
In strain: F18; resistant to streptomycin.
In strain: TCVGH25; probably resistant to streptomycin.
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Abolishes autophosphorylation as well as dephosphorylation of phospho-MprA.
Signal transduction histidine-protein kinase/phosphatase MprB
Dormancy associated translation inhibitor
Carbamoyl-phosphate synthase large chain
60% decrease in phosphorylation by PknH.
D-alanyl-D-alanine carboxypeptidase DacB1
Probable transcriptional regulator WhiB7
Probable diacyglycerol O-acyltransferase tgs1
HTH-type transcriptional regulatory protein RaaS
Reduces DNA binding affinity and ligand binding.
DNA-directed RNA polymerase subunit alpha
Increases nucleophilicity of active site Cys
Probable glycine dehydrogenase (decarboxylating)
Cobalamin-independent methionine synthase MetE C-terminal/archaeal
N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
Uncharacterized MFS-type transporter Rv0037c
Medium/long-chain-fatty-acid--CoA ligase FadD17
Putative quercetin 2,3-dioxygenase Rv0181c
Sulfate/thiosulfate import ATP-binding protein CysA
Constitutive interaction with RsbW; leads to active SigF.
Cytochrome bc1 complex Rieske iron-sulfur subunit
Electron transfer flavoprotein subunit beta
Encapsulin nanocompartment protein Rv1762c
Probable long-chain-fatty-acid--AMP ligase FadD30
Uncharacterized methyltransferase Rv0224c
Probable zinc-binding alcohol dehydrogenase Rv1895
Resuscitation-promoting factor core lysozyme-like
Does not affect phosphorylation by PknB. Does not affect transcriptional activity.
Does not affect phosphorylation by PknB. Inactivates transcriptional activity.
Phosphothreonine; by PknB; in inhibited form
Significantly reduces phosphorylation by PknB. Inactivates transcriptional activity.
Significantly reduces phosphorylation by PknB. Inactivates transcriptional activity. Significantly reduces the binding affinity of RegX3 for its target DNA sequence.
Slightly reduces phosphorylation by PknB. Inactivates transcriptional activity. Significantly reduces the binding affinity of RegX3 for its target DNA sequence.
HTH-type transcriptional repressor Rv1353c
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0893c
D-alanyl-D-alanine carboxypeptidase DacB2
Glutamate-1-semialdehyde 2,1-aminomutase
Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
Cytokinin riboside 5'-monophosphate phosphoribohydrolase
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3787c
Uncharacterized PPE family protein PPE59
Succinate-semialdehyde dehydrogenase [NADP(+)] 1
Putative aminoglycoside phosphotransferase
Ribonucleoside-diphosphate reductase subunit beta nrdF2
HTH-type transcriptional regulator Rv1719
Uncharacterized HIT-like protein Rv0759c
Decreases stability of the covalent enzyme-meropenem adduct.
Uncharacterized PPE family protein PPE19
Uncharacterized HTH-type transcriptional regulator Rv0196
Coenzyme A biosynthesis bifunctional protein CoaBC
Phosphopantothenoylcysteine decarboxylase
Uncharacterized PE-PGRS family protein PE_PGRS44
Putative acyl-[acyl-carrier-protein] desaturase DesA1
Putative acyl-[acyl-carrier-protein] desaturase DesA2
Molybdopterin synthase catalytic subunit 1
Does not affect phosphorylation. Lack of phosphorylation; when associated with A-171 and A-173.
Lack of phosphorylation; when associated with A-168 and A-171.
Lack of phosphorylation; when associated with A-168 and A-173.
Branched-chain-amino-acid aminotransferase