Mannose-6-phosphate isomerase type II C-terminal
Does not affect catalytic activity, but abolishes absorption at 300-500 nm.
Schiff-base intermediate with dihydroxyacetone-P
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
UDP-N-acetyl-D-mannosamine dehydrogenase
Signal recognition particle 54 kDa protein
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic
Does not reconstitute RNase P activity. Protein destabilized.
Probable threonylcarbamoyladenosine tRNA methylthiotransferase
DNA-directed RNA polymerase subunit Rpo11
DNA mismatch repair proteins mutS family
Pyridoxal 5'-phosphate synthase subunit PdxT
Tungsten-containing aldehyde ferredoxin oxidoreductase
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
Peptidase A24A-predicted C-terminal archaea
DNA-directed RNA polymerase subunit Rpo1C
tRNA-5-methyluridine(54) 2-sulfurtransferase
Dinitrogenase iron-molybdenum cofactor biosynthesis
Putative glycogen debranching enzyme N-terminal
O-(5'-phospho-DNA)-tyrosine intermediate
Glutamyl-tRNA(Gln) amidotransferase subunit D
tRNA (cytidine(56)-2'-O)-methyltransferase
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Ketoisovalerate oxidoreductase subunit VorB
Ketoisovalerate oxidoreductase subunit VorD
Phenylalanine--tRNA ligase alpha subunit
Alpha-D-phosphohexomutase alpha/beta/alpha
Formylmethanofuran dehydrogenase subunit E
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Thiamine pyrophosphate enzyme TPP-binding
Uncharacterized HTH-type transcriptional regulator PH0061
Removed in mature form; by autocatalysis
Major facilitator superfamily (MFS) profile
2-(3-amino-3-carboxypropyl)histidine synthase
Still able to bind a 4Fe-4S cluster. Less active than wild-type.
Still able to bind a 4Fe-4S cluster. Less active than wild-type. Not able to bind a 4Fe-4S cluster and loss of catalytic activity; when associated with Ala-287.
Still able to bind a 4Fe-4S cluster. Less active than wild-type. Not able to bind a 4Fe-4S cluster and loss of catalytic activity; when associated with Ala-59.
Uncharacterized HTH-type transcriptional regulator PH1013
Aspartate/homoserine dehydrogenase NAD-binding
Probable ABC transporter permease protein PH1216
Indolepyruvate oxidoreductase subunit IorA
Molybdate/tungstate import ATP-binding protein WtpC
Cobalamin-independent methionine synthase MetE N-terminal
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
EMO_00033 electrostatic stabiliser,EMO_00033 electrostatic stabiliser,EMO_00033 electrostatic stabiliser,EMO_00033 electrostatic stabiliser
EMO_00068 proton donor,EMO_00054 nucleophile,EMO_00058 nucleofuge,EMO_00066 proton acceptor
EMO_00068 proton donor,EMO_00066 proton acceptor,EMO_00066 proton acceptor,EMO_00068 proton donor
EMO_00116 metal ligand,EMO_00116 metal ligand,EMO_00116 metal ligand,EMO_00116 metal ligand
Translation initiation factor 2 subunit beta
Ketoisovalerate oxidoreductase subunit VorA
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Tryptophan synthase beta chain-like PALP
Aldehyde ferredoxin oxidoreductase N-terminal
N(4)-bis(aminopropyl)spermidine synthase C-terminal
Glycosyltransferase subfamily 4-like N-terminal
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Putative ABC transporter ATP-binding protein PH0132
Does not affect ability to stimulate RtcB ligase activity but has an effect on ATP utilization by RtcB.
Impaired ability to stimulate RtcB ligase activity.
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2
Putative 1-aminocyclopropane-1-carboxylate deaminase
Significant deacylation of correctly charged L-alanyl-tRNA(Ala) occurs.
Galactose-1-phosphate uridyl transferase C-terminal
Galactose-1-phosphate uridyl transferase N-terminal
Probable ABC transporter permease protein PH1215
Extradiol ring-cleavage dioxygenase class III enzyme subunit B
Dolichyl-phosphooligosaccharide-protein glycotransferase 2
Interacts with target acceptor peptide in protein substrate
Glyceraldehyde-3-phosphate dehydrogenase
Peptidase S9 prolyl oligopeptidase catalytic
Na+/H+ antiporter MnhB subunit-related protein
DNA polymerase II large subunit, 1st part
DNA polymerase II large subunit, 2nd part
Fe-S hydro-lyase tartrate dehydratase beta-type catalytic
Phosphoadenosine phosphosulphate reductase
Geranylgeranylglyceryl phosphate synthase
Uncharacterized HTH-type transcriptional regulator PH1692
Polysaccharide biosynthesis protein C-terminal
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1
DNA-directed RNA polymerase subunit 2 hybrid-binding
rRNA small subunit methyltransferase F RNA-binding PUA-like
Translation initiation factor 2 subunit alpha
Phosphoribosylformylglycinamidine synthase subunit PurQ
Protein-glutamate methylesterase/protein-glutamine glutaminase
3-isopropylmalate dehydratase small subunit
Translation initiation factor 2 subunit gamma
Indolepyruvate oxidoreductase subunit IorB
Resolvase/invertase-type recombinase catalytic
Bacterial type II secretion system protein E
tRNA (pseudouridine(54)-N(1))-methyltransferase
Putative 6-carboxy-5,6,7,8-tetrahydropterin synthase
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Plays an important role in substrate specificity
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Water stress and hypersensitive response
NADH:ubiquinone oxidoreductase 30kDa subunit
Ribosome maturation protein SDO1 homolog
Uncharacterized HTH-type transcriptional regulator PH0045
Aspartate carbamoyltransferase regulatory chain
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Methylated-DNA--protein-cysteine methyltransferase
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Probable ABC transporter permease protein PH1036
Phosphatidic acid phosphatase type 2/haloperoxidase
Digeranylgeranylglyceryl phosphate synthase
Non-specific cleavage pre-16S-rRNA, maybe due to altered substrate-binding.
Putative methylthioribose-1-phosphate isomerase
Glycoside hydrolase family 57 N-terminal
tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS
Uncharacterized ABC transporter extracellular-binding protein PH1214
Pyridine nucleotide-disulphide oxidoreductase dimerisation
tRNA N6-adenosine threonylcarbamoyltransferase
Plays critical role in maintaining high affinity for the substrate
Reduces the specific activity. Increases KM for L-ornithine.
Slightly increases the specific activity. Increases KM for L-ornithine.
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Transcription regulator AsnC/Lrp ligand binding
Decreased dependence of aspartate binding on Na(+) concentration.
Increased rate of aspartate transport; when associated with R-395.
Increased rate of aspartate transport; when associated with S-276.
Strongly decreased affinity for aspartate.
Strongly decreased chloride conductance.
1,4-alpha-glucan branching enzyme C-terminal
Cysteine sulfenic acid (-SOH) intermediate
Formate-dependent phosphoribosylglycinamide formyltransferase
Probable glycine dehydrogenase (decarboxylating) subunit 1
Methylene-tetrahydrofolate reductase C-terminal
Proliferating cell nuclear antigen PCNA N-terminal
Probable chemoreceptor glutamine deamidase CheD
DNA-directed RNA polymerase subunit Rpo5
Probable ribosomal RNA small subunit methyltransferase A
Phosphoribosylformylglycinamidine cyclo-ligase
Putative HTH-type transcriptional regulatory protein PH1808
Putative translation initiation factor eIF-2B subunit 2-like
Uncharacterized HTH-type transcriptional regulator PH1055
UDP-glucose/GDP-mannose dehydrogenase C-terminal
GMP synthase [glutamine-hydrolyzing] subunit B
Probable Brix domain-containing ribosomal biogenesis protein
About 65% GTPase activity; when associated with 243-Q--Q-250. About 35% GTPase activity; when associated with 217-Q--Q-224.
About 65% GTPase activity; when associated with 272-Q--Q-279. About 35% GTPase activity; when associated with 217-Q--Q-224.
Binds less L12, about 50% GTPase activity, about 35% GTPase activity; when associated with 243-Q--Q250 or 272-Q--Q-279.
Required for interaction with ribosomal protein L12 dimers
Probable cyclic pyranopterin monophosphate synthase
Pyruvate/ketoisovalerate oxidoreductases common subunit gamma
Glycoside hydrolase family 65 C-terminal
Glycoside hydrolase family 65 N-terminal
Glycoside hydrolase family 65 central catalytic
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
SAM-dependent methyltransferase TRM5/TYW2-type
Aminoacyl-transfer RNA synthetases class-II family profile
NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal
3-isopropylmalate dehydratase large subunit
Phosphoribosylformylglycinamidine synthase subunit PurL
Probable cobalamin biosynthesis protein CobD
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Probable ABC transporter permease protein PH1038
Putative transcriptional regulatory protein PH0763
Probable glycine cleavage system H protein
Probable glycine dehydrogenase (decarboxylating) subunit 2
Trk system potassium uptake protein TrkA homolog
Putative [LysW]-lysine/[LysW]-ornithine hydrolase
DNA double-strand break repair protein Mre11
Nicotinamide-nucleotide adenylyltransferase
Uncharacterized ATP-binding protein PH0541
Signal recognition particle 19 kDa protein
Uncharacterized HTH-type transcriptional regulator PH1916
Molybdate/tungstate-binding protein WtpA
Ribosomal RNA small subunit methyltransferase Nep1
tRNA (uracil(54)-C(5))-methyltransferase
DNA-directed RNA polymerase subunit Rpo3
2-fold decrease in the catalytic efficiency and strong decrease in the binding affinity.
Almost the same catalytic efficiency and binding affinity than wild-type.
Plays a direct or indirect catalytic role
Strong decrease in the catalytic efficiency and increase in the binding affinity.
Probable S-adenosyl-L-methionine-binding protein PH1056
3-methyl-2-oxobutanoate hydroxymethyltransferase
DNA-directed RNA polymerase subunit Rpo6
120-fold decrease in catalytic efficiency.
4000-fold decrease in catalytic efficiency.
Almost complete loss of enzymatic activity.
Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom
Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.
Adenosylcobinamide-GDP ribazoletransferase
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
O-(3'-phospho-DNA)-tyrosine intermediate
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Pyruvate/ketoisovalerate oxidoreductase catalytic
Putative ABC transporter ATP-binding protein PH1815
Probable molybdenum cofactor guanylyltransferase
DNA double-strand break repair Rad50 ATPase
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
Putative CRISPR-associated endoribonuclease-like protein Cas6nc
DNA-directed RNA polymerase subunit Rpo10
Putative gamma-glutamylcyclotransferase PH0828
UPF0173 metal-dependent hydrolase PH1671
Digeranylgeranylglycerophospholipid reductase
Involved in ionization of N3 of dUMP, leading to its activation
Uncharacterized ZPR1-like protein PH1223
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
DNA-directed RNA polymerase subunit Rpo12