Impairs the interaction with APM2 and its recruitment to membranes, and leads to sertraline sensitivity.
Restriction of telomere capping protein 4
Glycine cleavage system H protein, mitochondrial
Probable ferric reductase transmembrane component 8
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
In strain: A2-M8; confers resistance to the allylamine antifungal terbinafine.
Disrupts binding to FFAT motif and causes OPI1 mislocalization.
Disrupts binding to FFAT motif; when associated with D-84.
Disrupts binding to FFAT motif; when associated with D-86.
Helical; Anchor for type IV membrane protein
Reduces binding to FFAT motif and impairs OPI1 function.
Vesicle-associated membrane protein-associated protein SCS2
Dehydrodolichyl diphosphate synthase complex subunit SRT1
ISWI chromatin-remodeling complex ATPase ISW1
Does not alter the arsenite/proton exchange across the plasma membrane.
Impairs cell membrane localization, and leads to arsenite sensitivity.
Leads to ER retention and arsenite sensitivity.
Leads to ER retention, protein unstability and arsenite sensitivity.
Leads to complete loss of metalloid transport function.
Results in moderate reduction of arsenite transport capacities and sorting perturbations.
Uncharacterized membrane protein YDL218W
Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype.
Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype.
Putative uncharacterized protein YHR219W
Defect in snoRNA accumulation. Growth defect at 37 degrees Celsius.
Does not enable copper chaperone-independent activation.
Enables copper chaperone-independent activation; when associated with A-143 or with S-143.
Enables copper chaperone-independent activation; when associated with A-145 or with L-145.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Interchain (with C-229 in CCS1); in linked form
Putative uncharacterized protein YJR120W
Y' element ATP-dependent helicase protein 1 copy 4
Biogenesis of lysosome-related organelles complex 1 subunit BLS1
Mimics acetylation; does not show increased sensitivity to DNA damaging agents.
Mimics glutarylation; delays in cell proliferation; increased sensitivity to DNA damaging agents.
Mimics unmodified residue; does not show increased sensitivity to DNA damaging agents.
Gly-Pro-Asn (GPN)-loop; involved in dimer interface
Impairs heterodimer formation with NPA3/GPN1.
Stabilizes the phosphate intermediate; shared with dimeric partner
Chromatin-remodeling complexes subunit NGG1
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163.
Altered inheritance of mitochondria protein 9, mitochondrial
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Blocks autocatalytic processing of the precursor protein.
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Decreases the amount of 40S ribosomal subunit; when associated with R-144; A-244 and A-246.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; A-244 and A-246.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; R-144 and A-244.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; R-144 and A-246.
Abolished GTP-binding and ability to trigger the No-Go Decay (NGD) pathway and promote degradation of non-functional rRNAs.
Abolished ability to trigger the No-Go Decay (NGD) pathway, without affecting the ability to promote degradation of non-functional rRNAs.
Loss of function. Abolished GTP-binding and ability to trigger the No-Go Decay (NGD) pathway and promote degradation of non-functional rRNAs.
ATP synthase subunit gamma, mitochondrial
In ATP3-1; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA.
In ATP3-5; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA.
Required for respiratory growth protein 9, mitochondrial
Prevents insertion into the outer membrane and increases the efficiency of import into the intermembrane space.
Centromere DNA-binding protein complex CBF3 subunit C
Target of rapamycin complex 1 subunit TCO89
Thiamine metabolism regulatory protein THI3
Vacuolar protein sorting-associated protein 62
Loss of aminopeptidase I precursor maturation and no more association with vacuole and punctate structures.
Impairs interaction with HSL7 and prevents bud neck localization and degradation.
Alters kinase activation and biological activity, including enhanced allosteric subunit associations and increased oxidative stress resistance and life span; when associated with A-357.
Alters kinase activation and biological activity, including enhanced allosteric subunit associations and increased oxidative stress resistance and life span; when associated with I-367.
Carbon catabolite-derepressing protein kinase
Exhibits greater activity than wild-type SNFl in an immune complex assay where other associated molecules are present, but exhibits the same activity in a protein blot assay.
Inactivates the kinase activity without affecting protein levels.
Cytochrome c oxidase subunit 4, mitochondrial
Uncharacterized membrane protein YNL019C
2 X 20 AA approximate tandem repeats, Ser-rich
3 X 51 AA approximate repeats, Ser/Thr-rich
8 X 45 AA approximate tandem repeats, Thr-rich
PAN2-PAN3 deadenylation complex catalytic subunit PAN2
Translationally-controlled tumor protein homolog
Isocitrate dehydrogenase [NADP] cytoplasmic
Putative transcriptional regulatory protein YJL206C
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial
Loss of acireductone dioxygenase activity.
No loss of acireductone dioxygenase activity.
Cytochrome b-c1 complex subunit 6, mitochondrial
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial
Maintenance of telomere capping protein 7
Transcription initiation factor TFIID subunit 3
Peptidyl-prolyl cis-trans isomerase CYP7
Vacuolar import and degradation protein 22
Activates HOG1 in a constitutive manner, without the need of a stimulating stress.
Impairs catalytic activity and nuclear translocation.
Impairs catalytic activity, expression of CTT1 and increases sensitivity to osmotic shock.
Impairs catalytic activity, nuclear translocation, expression of CTT1 and increases sensitivity to osmotic shock.
Impairs expression of CTT1 and increases sensitivity to osmotic shock.
Antagonist of mitotic exit network protein 1
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2
Abolishes SKT5 prenylation. Decreases chitin synthase CHS3 activity. Decreases chitin chain length. Decreases cellular chitin level. Normal SKT5 localization to cellular membranes. Resistance to Calcofluor White (cell wall stressor).
Decreases chitin synthase CHS3 activity, resistance to Calcofluor White (cell wall stressor), decreases cellular chitin level.
Impairs interaction with ubiquitin; when associated with A-109.
Impairs interaction with ubiquitin; when associated with A-110.
Impairs interaction with ubiquitin; when associated with A-135.
Impairs interaction with ubiquitin; when associated with A-136.
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with D-60 and V-142.
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with D-60 and V-68.
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with V-68 and V-142.
In strain: V1-09, YJM269, YJM270, YJM326, YJM339, YJM627 and YJM1129.
In strain: YJM 69, YJM270, YJM326 and YJM1129.
In strain: YJM269, YJM270, YJM280, YJM320, YJM326 and YJM1129.
In strain: YJM269, YJM270, YJM326 and YJM1129.
Mitochondrial import receptor subunit TOM40
Putative uncharacterized protein YOR186W
Loss of MCM2-7 complex helicase activity.
Increased protein secretion most likely due to reduced catalytic activity. Cells are larger and clump together.
Chromatin modification-related protein EAF6
Sphingolipid long chain base-responsive protein LSP1
Uncharacterized membrane protein YPR071W
Impairs interaction with ATG8 and decreases subsequent reticulophagy.
Ccompletely abolished H3K4 di- and trimethylation and greatly reduced H3K4 monomethylation.
Substantially lowers H3K4me3 levels in yeast, while H3K4me1 and H3K4me2 are marginally affected.
Impairs the ability to arrest the cell cycle.
Uncharacterized membrane protein YIL054W
Sensitive in DMS protection of U1 snRNA.
Sensitive in DMS protection of U1 snRNA; when associated with 6-I-N-7.
Sensitive in DMS protection of U1 snRNA; when associated with 65-D--L-67.
Sensitive in DMS protection of U1 snRNA; when associated with 74-R-G-75.
Sensitive in DMS protection of U1 snRNA; when associated with 79-V-I-80.
Sensitive in DMS protection of U1 snRNA; when associated with I-4.
Sensitive in DMS protection of U1 snRNA; when associated with I-63.
Splicing defects; when associated with 230-F--T-232.
Splicing defects; when associated with 262-D-I-263.
Splicing defects; when associated with 291-A--K-293.
Splicing defects; when associated with F-268.
Splicing defects; when associated with I-228.
Splicing defects; when associated with S-295.
Abolishes short-term enzyme inactivation by glucose addition.
Reduces activity by 45%; when associated with L-220.
Reduces activity by 45%; when associated with R-216.
L-threo-3-hydroxyaspartate ammonia-lyase
In strain: MMW1-15h2, MMW1-2h2, Sgu52E and Sgu52F.
Leucine zipper-like heptad repeat, required for interaction with NSP1
In SRB6-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.
Mediator of RNA polymerase II transcription subunit 22
Conserved oligomeric Golgi complex subunit 1
DNA-directed RNA polymerase I subunit RPA135
Lethal. Abolishes recruitment of RPA1 to Pol I. No effect; when associated with A-1104; A-1128 and A-1131.
No effect; when associated with A-1104; A-1107 and A-1128.
No effect; when associated with A-1104; A-1107 and A-1131.
No effect; when associated with A-1107; A-1128 and A-1131.
Responsible of suppression of RPA190-5 and RPA190-1 mutations.
In MHR1-1; causes a defect in the partitioning of nascent mtDNA into buds and delays generation of homoplasmic cells.
High-osmolarity-induced transcription protein 1
Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410.
Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410.
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54
Impairs the formation of protofilaments; when associated with K-90. Also impairs the formation of protofilaments; when associated with E-94. Also impairs the formation of protofilaments; when associated with E-107. Also impairs the formation of protofilaments; when associated with E-114.
Leads to severe sorting defects. Impairs the formation of protofilaments; when associated with E-52.
Leads to severe sorting defects; when associated with E-25 and E-29.
Leads to severe sorting defects; when associated with E-25 and E-36.
Leads to severe sorting defects; when associated with E-29 and E-36.
Leads to severe sorting defects; when associated with K-102 and K-109.
Leads to severe sorting defects; when associated with K-95 and K-102.
Leads to severe sorting defects; when associated with K-95 and K-109.
Cells are able to grow on a medium with oleate as a sole carbon source.
Cells are unable to grow on a medium with oleate as a sole carbon source.
In Bmut mutant; abolished ATPase activity of the PEX1-PEX6 AAAATPase complex.
In PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. In Amut mutant; abolished ATPase activity of the PEX1-PEX6 AAAATPase complex.
In PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes.
Abolishes ubiquitination by HEL2. Defective activation of the ribosome quality control (RQC) pathway. Sensitive to anisomycin (stalls ribosomes in the rotated state).
UDP-N-acetylglucosamine transferase subunit ALG14
Ammonia regulation of amino acid uptake protein
Vacuolar protein sorting-associated protein 72
Putative uncharacterized protein YOL114C
Y' element ATP-dependent helicase protein 1 copy 2
No growth at 13 degrees Celsius; when associated with D-305.
No growth at 13 degrees Celsius; when associated with G-637.
No growth at 13 degrees Celsius; when associated with P-564.
No growth at 13 degrees Celsius; when associated with Q-431 and G-472.
No growth at 13 degrees Celsius; when associated with T-191 and G-472.
No growth at 13 degrees Celsius; when associated with T-191 and Q-431.
No growth at 13 degrees Celsius; when associated with V-260.
No growth at 13 degrees Celsius; when associated with V-291.
No growth at 13 degrees Celsius; when associated with V-306.
Abolishes dimer formation. Decreases activity and binding to RTT109.
Decreases RTT109 binding and activity stimulation.
Increases acetylation of histone H3 'Lys-56'.
Mildly decreases RTT109 activity stimulation.
Vacuolar protein sorting-associated protein 75
Proton donor; shared with dimeric partner
Stacks against the aromatic ring of pyridoxal phosphate and stabilizes reaction intermediates
Disrupts association with the translocase complex.
Mitochondrial import inner membrane translocase subunit TIM44
Non-structural maintenance of chromosome element 4
Slightly reduces affinity for poly-URNA ends.
U6 snRNA-associated Sm-like protein LSm4
RNA polymerase II transcriptional coactivator SUB1
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI13
CCCFC; essential for catalytic activity, may be the site of iron coordination
Probable proteasome subunit alpha type-7
C-terminal-extended nuclear localization signal
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the 60S subunit; when associated with E-95.
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the 60S subunit; when associated with E-98.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-289 and A-290.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-289 and A-295.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-290 and A-295.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-334.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with e-332.
Significantly diminished nuclear localization; when associated with A-314 and A-315.
Significantly diminished nuclear localization; when associated with A-314 and A-319.
Significantly diminished nuclear localization; when associated with A-315 and A-319.
RNA polymerase I-specific transcription initiation factor RRN3
Ubiquitin-conjugating enzyme suppressor 1
Mediator of RNA polymerase II transcription subunit 31
Mediator of RNA polymerase II transcription subunit 14
Protoheme IX farnesyltransferase, mitochondrial
General control transcription factor GCN4
In strain: CLIB 388, CLIB 410, CLIB 413, CLIB 630 haplotype Ha1, K1, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1.
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13 haplotype Ha2, Sigma 1278B haplotype Ha1, YIIc12 and YIIc17.
Reduces transcriptional activation activity; when associated with A-107; A-110; A-113; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-110 and A-113.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-110; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-113; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-110; A-113; A-120; A-123 and A-124.
Oxygen-dependent coproporphyrinogen-III oxidase
Abolishes peptidase activity, which also hinders autocatalytic processing of the enzyme to the mature form.
In GAL6DB; disrupts nucleic acid-binding activity, but retains normal peptidase activity.
Removed in mature form; by autocatalysis
Results in the abnormal cleavage of 3 C-terminal residues instead of only 1 during autocatalytic processing.
Dominant negative mutant; cells show a severe synthetic sick phenotype on glycerol/lactate medium.
Loss of function. Abolished homodimerization.
Involved in binding clathrin heavy chain
Chromatin structure-remodeling complex protein RSC6
Defective in spindle pole body duplication at 37 degrees Celsius.
Defective in spindle pole body duplication at 37 degrees Celsius; when associated with L-572.
Defective in spindle pole body duplication at 37 degrees Celsius; when associated with L-573.
Impairs the localization to the nuclear inner membrane.
In mps3-1; defective in spindle pole body assembly and duplication.
In nep98-7; defective in spindle organization during nuclear division and in G2/M progression.
Lethal; when associated with A-494; A-495; A-496 and A-497.
Lethal; when associated with A-494; A-495; A-496 and A-498.
Lethal; when associated with A-494; A-495; A-497 and A-498.
Lethal; when associated with A-494; A-496; A-497 and A-498.
Lethal; when associated with A-495; A-496; A-497 and A-498.
Mimics constitutive phosphorylation and blocks the regulation of the localization to nuclear inner membrane.
Spindle pole body assembly component MPS3
Completely inactive as thiol transferase. No activity recovered against 1-chloro-2,4-dinitrobenzene (CDNB).
No effect on thiol transferase activity.
No effect on thiol transferase activity. No effect on thiol transferase activity; when associated with D-173.
Cytochrome b-c1 complex subunit 2, mitochondrial
Impairs sumoylation; when associated with R-201.
Impairs sumoylation; when associated with R-215.
Chromatin structure-remodeling complex protein RSC14
Mitochondrial outer membrane protein OM14
No effect on NTP-binding and hydrolysis. Resistant to DNA damage.
Reduces NTP-binding and hydrolysis. Shows DNA damage sensitivity.
Induces an increase of mitochondrial fission.
Eukaryotic translation initiation factor 2 subunit gamma
In SUI4; defective in ternary complex formation, correlating with a higher rate of dissociation from charged initiator-tRNA in the absence of GTP hydrolysis.
Increases the off-rate for GDP, without altering the apparent dissociation constant for Met-tRNAi(Met). Mimicks the function of the guanine nucleotide exchange factor eIF-2B.
Reduces the affinity of eIF-2 for Met-tRNAi(Met) without affecting the k(off) value for guanine nucleotides.
In APC11-13; G2/M cell cycle arrest at 37 degrees Celsius.
Trafficking protein particle complex II-specific subunit 120
DNA-directed RNA polymerase III subunit RPC4
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1
Abolishes ATPase activity and proton transport, but does not affect complex assembly.
Reduces assembly of V-ATPase complexes and reduces ATPase activity of the assembled complexes.
V-type proton ATPase subunit a, vacuolar isoform
Putative uncharacterized protein YOL024W
Abolishes mitochondrial localization and activity, but not cytoplasmic activity of the enzyme.
RNA polymerase II transcription factor B subunit 3
Decreases propensity to stall ribosomes.
The nascent chain stimulates ribosomal stalling during translation by interfering with the conformation of the peptidyl transferase center (PTC), and the translating mRNA by adopting a difficult-to-decode structure at the ribosome decoding center
Allows for altered tRNA substrate recognition.
Significantly affects the G(-1) addition.
Translation initiation factor eIF-2B subunit alpha
Vacuolar protein sorting-associated protein 5
In PRP43-DN1; dominant-negative phenotype.
In PRP43-DN2; dominant-negative phenotype.
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43
Putative mitochondrial carrier protein PET8
Trafficking protein particle complex subunit BET3
In SEC16-1; ts accumulation of ER membranes.
In SEC16-2; ts accumulation of ER membranes.
In SEC16-3; ts accumulation of ER membranes.
In SEC16-4; ts accumulation of ER membranes.
Poisonous alpha-tubulins that cause lethality. Microtubules do not depolymerize.
Strongly reduces formation of the thioester intermediate with ubiquitin.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with K-1014.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with K-1016.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with P-912.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with P-914.
Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport.
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
Abolishes acetylation and association with cis-Golgi.
Cytochrome c oxidase subunit 5A, mitochondrial
Abolishes agglutination; when associated with S-25.
Abolishes agglutination; when associated with S-68.
Abolishes processing of CYB2 and COX2 and presence of SOM1 in the IMP complex.
In pet ts2858; temperature-sensitive; abolishes processing of CYB2 and presence of SOM1 in the IMP complex.
Mitochondrial inner membrane protease subunit 1
Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate.
DNA replication fork-blocking protein FOB1
Phosphoribosylaminoimidazole carboxylase
Y' element ATP-dependent helicase YML133C
Putative uncharacterized protein YAL037C-A
Abolished ability to mediate translation termination. Can recognize stop codons in ribosomal A-site, but is unable to catalyze peptidyl-tRNA hydrolysis.
Eukaryotic peptide chain release factor subunit 1
Abolishes acetyltransferase activity; but not chromatid cohesion activity.
Dual specificity protein phosphatase PPS1
Chromatin modification-related protein EAF5
Inositol auxotrophy. Impaired ER morphology and aberrant lipid droplet (LD) budding.
For protease activity; shared with dimeric partner
Accumulation of aberrant 22S and 23S rRNA intermediates.
Decreases cell population growth; when associated with S-222 and D-237.
Does not appear to affect its physical interaction with ANY1. Increases localization to late Golgi compartments. Decreases cell population growth; when associated with V-228 and D-237.
Localization to the Golgi apparatus appears normal.
May enhance substrate loading. Decreases cell population growth; when associated with V-228 and D-237.
No apparent effect on viability. Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin) and papuamide A (phosphatidylserine-binding cytotoxin). Localization to the Golgi apparatus appears normal.
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin) and papuamide A (phosphatidylserine-binding cytotoxin).
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin) and papuamide A (phosphatidylserine-binding cytotoxin). Localization to the Golgi apparatus appears normal.
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin) and papuamide A (phosphatidylserine-binding cytotoxin). Localization to the Golgi apparatus appears normal. Decreases cell population growth; when associated with S-222 and V-228.
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin).
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin). Localization to the Golgi apparatus appears normal.
Sensitive to neomycin and trifluoperazine; sensitivity is suppressed by knockout of ANY1.
Sensitive to papuamide A (phosphatidylserine-binding cytotoxin). Localization to the Golgi apparatus appears normal.
Ribonucleases P/MRP protein subunit POP1
Uncharacterized vacuolar membrane protein YGR125W
Cytochrome c oxidase-assembly factor COX23, mitochondrial
DNA replication complex GINS protein PSF3
Causes defects in ATP-dependent dissociation of MutS alpha from mismatch DNA and in interactions between MutS alpha and MutL alpha. Defective in MMR, but not in NHTR.
Causes strong defects in MutS alpha mismatch binding. Defective in MMR, but not in NHTR.
Disrupts MutS alpha ATPase activity, but does not affect ATP binding or interactions with MutL alpha. Defective in MMR, but not in NHTR.
Fully functional in a mismatch repair assay.
Has no defect in mismatch DNA binding, but lacks ATP-induced conformational change. Defective in MMR and in NHTR.
Impairs ATP binding; reduces catalytic activity 1.6-fold for ATP hydrolysis. Has a dominant negative mutator effect.
Moderately reduced activity in a mismatch repair assay.
Partially defective in a mismatch repair assay.
Reduces catalytic activity 50-fold for ATP hydrolysis.
Decreases glucosylceramide and phosphatidylethanolamine uptake, but not phosphatidylcholine uptake.
Decreases glucosylceramide, phosphatidylethanolamine, and phosphatidylcholine uptake.
Decreases phosphatidylcholine and increases phosphatidylserine uptake.
Decreases phosphatidylcholine and phosphatidylehtanolamine uptake.
Decreases phosphatidylcholine and phosphatidylethanolamine uptake, and increases phosphatidylserine uptake.
Decreases phosphatidylcholine and phosphatidylethanolamine uptake.
Decreases phosphatidylcholine and phosphatidylserine uptake.
Decreases phosphatidylcholine uptake, but not phosphatidylethanolamine uptake.
Decreases phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine uptake.
Does not alter phosphatidic acid or sphingomyelin uptake.
Increases glucosylceramide, phosphatidylethanolamine, and phosphatidylcholine uptake.
Increases phosphatidylcholine and phosphatidylserine uptake.
Increases phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and sphingomyelin uptake, but not phosphatidic acid uptake.
Increases phosphatidylserine uptake but not phosphatidic acid or sphingomyelin uptake.
Increases phosphatidylserine, phosphatidylcholine, and sphingomyelin uptake.
Involved in phosphatidylcholine substrate selection
Involved in the release of the transported lipid into the cytosolic leaflet
Leads to a 40-fold decrease of affinity to PIP2.
Leads to a slight reduction of PIP2 binding.
Loss of recruitment to vacuole membrane.
Raises KM for malonyl-CoA by a factor of 15.
Raises KM for malonyl-CoA by a factor of 20.
Raises KM for malonyl-CoA by a factor of 70.
DNA-directed RNA polymerase, mitochondrial
ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Phosphoadenosine phosphosulfate reductase
Vacuolar protein sorting-associated protein 53
Mediator of RNA polymerase II transcription subunit 3
Abolishes cleavage of substrate oligonucleotides.
Cannot properly relocalize in response to oxidative stress.
In NTG1(mts); reduces mitochondrial localization by 40%.
In NTG1(nls1); reduces nuclear localization by 60%.
In NTG1(nls2); reduces nuclear localization by 60%.
Pyruvate dehydrogenase complex protein X component, mitochondrial
Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-827.
Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-829.
Impairs deubiquitination activity and binding to BRO1; when associated with A-827 and A-829.
Impairs deubiquitination activity and protein sorting into the MVB pathway.
Phosphatidyl-N-methylethanolamine N-methyltransferase
In TOR2-1; confers resistance to rapamycin.
DNA polymerase epsilon catalytic subunit A
In POL2-18; temperature-sensitive mutant.
In POL2-9; temperature-sensitive mutant.
Increases rates of C-to-A transversion substitutions.
RNA-binding protein RMD9-like, mitochondrial
5 X 12 AA approximate tandem repeats, Ala/Ser-rich
5 X 6 AA approximate tandem repeats, Ala/Ser-rich
Y' element ATP-dependent helicase protein 1 copy 1
Y' element ATP-dependent helicase protein 1 copy 5
Y' element ATP-dependent helicase protein 1 copy 8
Impairs histone H3 phosphorylation and reduces transcription of some GCN5 regulated genes.
Increases sensitivity to genotoxic agents inducing DNA breaks during replication.
Impaired ability to rescue stalled ribosomes.
The measured mass is that of the monomethylated protein.
13 X 45 AA approximate tandem repeats, Thr-rich
Serine/threonine-protein kinase BCK1/SLK1/SSP31
Growth inhibited above 30 degrees Celsius.
Growth inhibited above 34 degrees Celsius.
Leads to mislocalization of NPCs and overproliferation of the nuclear and ER membranes at 34 degree Celsius.
Decreases the percentage of cells having vacuolar ATG8, impairs ATG9 recycling between the PAS and the cytoplasmic pool, and reduces autophagy by about 60%.
Phosphomimetic mutant that rescues autophagy.
Does not affect function; when associated with A-12; E-17 and D-22.
Does not affect function; when associated with D-16 and D-19.
Does not affect function; when associated with D-16 and D-20.
Does not affect function; when associated with D-19 and D-20.
Does not affect function; when associated with R-11; A-12 and D-22.
Does not affect function; when associated with R-11; A-12 and E-17.
Does not affect function; when associated with R-11; E-17 and D-22.
Mitochondrial distribution and morphology protein 12
Single-stranded DNA-binding protein RIM1, mitochondrial
Inositol phosphorylceramide synthase regulatory subunit KEI1
Upstream activation factor subunit UAF30
Eukaryotic translation initiation factor 4E
Confers resistance to cycloheximide, an inhibitor of polypeptide elongation.
30 X 2 AA approximate tandem repeats of Q-A
Decreases the interaction between the mediator complex and GCN4. Decreases transcription of GCN4-dependent targets. Sensitive to amino acid starvation.
Decreases transcription of GCN4-dependent targets. Decreases recruitment of the mediator complex to the upstream activating sequence (UAS) of amino-acid starvation responsive genes. Sensitive to amino acid starvation.
Mediator of RNA polymerase II transcription subunit 15
Putative serine/threonine-protein kinase YPL150W
Uncharacterized endoplasmic reticulum membrane protein YJR015W
Dihydrosphingosine 1-phosphate phosphatase YSR3
Stabilizes the active site histidine for nucleophilic attack
Probable serine/threonine-protein kinase FMP48
Vacuolar protein sorting-associated protein 35
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs).
Defective in ESCRT-I cargo sorting; reduces MVB12 localization to MVBs; abolishes interaction with MVB12; reduces interaction with SRN2.
No interaction of the ESCRT-I complex with ubiquitin.
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
In strain: SK1, V1-09, YJM230, YJM320 and YJM339.
In strain: YJM1129, YJM269, YJM270, YJM326 and YJM627.
General negative regulator of transcription subunit 5
6-phosphogluconolactonase-like protein 2
Expressed at lower levels. GTPase activity reduced.
Expressed at the same level as wild-type. GTPase and GDPase activities decreased more than 20-fold toward GTP and 3-fold for GDP.
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and A-261.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and R-162.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; R-162 and A-261.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with N-66; R-162 and A-261.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and G-207.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and S-95.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with S-95 and G-207.
Pore and endoplasmic reticulum protein of 33 kDa
Prevents interaction with GLC7 and reduces nuclear levels of GLC7 and SDS22.
Abolishes thiocarboxylation and function in 2-Thiolation of tRNA.
Increased recombination centers protein 22
ABC1 family protein YPL109C, mitochondrial
Impairs histone deacetylase activity and transcription repression.
Y' element ATP-dependent helicase protein 1 copy 6
Uncharacterized mitochondrial hydrolase FMP41
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
Mitochondrial group I intron splicing factor CCM1
Vacuolar protein sorting-associated protein 45
Loss of transport activity; when associated with D-112 and D-215. Restored transport activity; when associated with K-109; D-112; K-212; D-215 and K-306.
Loss of transport activity; when associated with D-112 and D-309. Restored transport activity; when associated with K-109; D-112; K-212; K-306 and D-309.
Loss of transport activity; when associated with D-215 and D-309. Restored transport activity; when associated with K-109; K-212; D-215; K-306 and D-309.
Loss of transport activity; when associated with K-109 and K-212. Restored transport activity; when associated with D-109; D-112; K-212; D-215 and D-309.
Loss of transport activity; when associated with K-109 and K-306. Restored transport activity; when associated with D-109; D-112; D-215; K-306 and D-309.
Loss of transport activity; when associated with K-212 and K-306. Restored transport activity; when associated with D-112; K-212; D-215; K-306 and D-309.
Monoisotopic mass with 2 methylation modifications.
Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55.
With N6-methyl-Lys-40 and N6-methyl-Lys-55.
Geranylgeranyl transferase type-2 subunit beta
Vacuolar membrane-associated protein IML1
Phosphatidylinositol transfer protein SFH5
Mitochondrial inner membrane magnesium transporter MFM1
3 X 19 AA tandem repeats of S-M-X-M-X-A-M-S-S-A-S-K-T-X-X-S-X-M-X
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Actin cytoskeleton-regulatory complex protein END3
In strain: SK1, YJM 269, YJM 270, YJM 280, YJM 320, YJM 326, YJM 339, YJM 421, YJM 627, YJM 789 and YJM 1129.
In strain: YJM 280, YJM 320, YJM 326, YJM 627, YJM 789 and YJM 1129.
Membrane-anchored lipid-binding protein YSP2
Peroxisome proliferation transcriptional regulator
Non-structural maintenance of chromosome element 5
Mitochondrial import inner membrane translocase subunit TIM54
Trafficking protein particle complex II-specific subunit 130
In DED1-120; impairs protein synthesis at 15 degrees Celsius; when associated with D-108.
In DED1-120; impairs protein synthesis at 15 degrees Celsius; when associated with D-494.
In DED1-18; impairs BMVRNA synthesis; when associated with D-317.
In DED1-18; impairs BMVRNA synthesis; when associated with M-237.
In DED1-199; impairs protein synthesis at 15 degrees Celsius.
Lethal in vivo and impairs ATPase and RNA-helicase activities in vitro.
Lethal in vivo and inhibits ATPase and helicase activities in vitro.
Reduces RNA-helicase activity about 2.5-fold in vitro.
Reduces RNA-helicase activity about 5-fold in vitro.
Slow growth at 18 and 30 degrees Celsius and no growth at 16 degrees Celsius.
Slow growth at 18 and 36 degrees Celsius in vivo, and reduces RNA-helicase activity about 5-fold in vitro.
Strongly reduces ATPase activity and strand displacement activity.
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
Multicopy suppressor of SEC21 protein 28
Helical; Signal-anchor for type II membrane protein
Guanine nucleotide exchange factor subunit RGP1
Nuclear polyadenylated RNA-binding protein 4
Vacuolar protein sorting-associated protein 29
27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase
DNA-directed RNA polymerase III subunit RPC10
Important for inositol polyphosphate binding
Vacuolar transporter chaperone 3 complex subunit 3
Putative elongation factor 1 gamma homolog
Cytosolic Fe-S cluster assembly factor NAR1
Impaired maturation of cytosolic Fe/S proteins. Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416.
Impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-177 or A-231.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-177.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-412.
Weakly reduced iron content and impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-62 or A-65.
Chromosome segregation in meiosis protein 2
U3 small nucleolar RNA-associated protein 5
Small glutamine-rich tetratricopeptide repeat-containing protein 2
Cu(2+) suppressing and bleomycin sensitive protein 1
O-(5'-phospho-DNA)-tyrosine intermediate
Loss of complementation of the suppression phenotype.
Serine/threonine-protein phosphatase PP1-1
Uncharacterized membrane protein YPR027C
General transcription and DNA repair factor IIH subunit TFB1
Abolishes enzyme activity; when associated with A-11 and A-505.
Abolishes enzyme activity; when associated with A-11 and A-511.
Abolishes enzyme activity; when associated with A-505 and A-511.
Causes accumulation in vacuoles and abolishes sorting into internal vesicles in late endosomes.
Guanine nucleotide exchange factor subunit RIC1
Probable ADP-ribose 1""-phosphate phosphatase YML087W
2-oxoglutarate dehydrogenase, mitochondrial
Eukaryotic translation initiation factor 3 subunit A
Reduces interaction of eIF-3 with the 40S ribosome.
Putative uncharacterized protein YCL021W-A
Abolishes ubiquitin chain elongation. No effect on thioester formation at the active site.
Lowers rate of ubiquitin chain elongation. No effect on thioester formation at the active site.
Strongly reduces MMS2 binding and interferes with error-free DNA repair.
Fatty acyl-CoA synthetase and RNA processing-associated kinase 1
Confers increased sensitivity to rapamycin and enhanced accumulation of glycogen upon TORC1 inhibition.
cAMP-dependent protein kinase regulatory subunit
Nuclear membrane organization protein APQ12
Pyridoxamine 5'-phosphate oxidase YLR456W homolog
Dehydrodolichyl diphosphate synthase complex subunit RER2
Abolished ability to act downstream of the RQT (ribosome quality control trigger) complex.
Decreased ability to participate to No-Go Decay (NGD) pathway in response to ribosome collision.
In CUE-D1 mutant; impaired ability to bind polyubiquitinated RPS7/eS7; when associated with 21-A-A-22.
In CUE-D1 mutant; impaired ability to bind polyubiquitinated RPS7/eS7; when associated with 46-A-A-47.
In CUE-D2 mutant; impaired ability to bind polyubiquitinated RPS7/eS7; when associated with 66-A-A-67.
In CUE-D2 mutant; impaired ability to bind polyubiquitinated RPS7/eS7; when associated with 93-A-A-94.
Impairs kinase activity; when associated with A-2224.
Impairs kinase activity; when associated with K-2229.
In MEC1-100; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-1179.
In MEC1-100; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-1700.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with P-552 and S-781.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-225 and P-552.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-225 and S-781.
In MEC1-85; disrupts interaction with RFA1 and severely impairs kinase activity.
In MEC1-87; decreases the level of MEC1 and impairs viability.
Sigma-like sequence protein 1, mitochondrial
DNA replication complex GINS protein PSF1
In PSF1-1; temperature-sensitive mutant. Defective in DNA replication. Impaired chromatin binding of CDC45.
Beta-glucan synthesis-associated protein KRE6
Decreases catalytic activity for L-tyrosine 400-fold, but allows the utilization of 3-iodo-L-tyrosine and other 3-modified tyrosines as substrates.
Impairs interaction with histones H3 and H4; when associated with F-187.
Impairs interaction with histones H3 and H4; when associated with F-354.
ER degradation-enhancing alpha-mannosidase-like protein 1
Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a A-511 mutation.
Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a R-524 mutation.
Instead of the formation of an intermediate complex with a thiol ester bond between ATG7 (E1-like enzyme) and ATG8 (substrate) or between ATG7 and ATG12 (substrate), a stable complex with an O-ester bond is formed. No more formation of ATG12-ATG5 conjugate.
Loss of interaction with ATG8 and ATG12 and no more formation of ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.
Loss of interaction with ATG8 and ATG12, and no more ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.
Loss of interaction with ATG8; when associated with A-466 and A-490.
Loss of interaction with ATG8; when associated with A-466 and F-486.
Loss of interaction with ATG8; when associated with F-486 and A-490.
Ubiquitin-like modifier-activating enzyme ATG7
ABC transporter ATP-binding protein/permease PDR18
V-type proton ATPase subunit a, Golgi isoform
Transcription initiation factor TFIID subunit 5
Cell cycle serine/threonine-protein kinase CDC5/MSD2
Dissociation of the elongator complex following assembly. Abolished interaction with KTI11 and KTI12.
Dissociation of the elongator complex following assembly. Loss of elongator complex activity; when associated with A-118.
Dissociation of the elongator complex following assembly. Loss of elongator complex activity; when associated with A-121.
Does not affect interaction with KTI11 and KTI12. Loss of elongator complex activity.
Eliminates iron contents; when associated with S-108 and S-118.
Eliminates iron contents; when associated with S-108 and S-121.
Eliminates iron contents; when associated with S-118 and S-121.
Impaired tRNA wobble uridine modification.
Inactivates catalytic activity and leads to substrate retention.
In cly5; temperature-sensitive mutation that cause cell lysis at high temperature.
In cly7; temperature-sensitive mutation that cause cell lysis at high temperature.
No effect on activity. Reduces affinity for ornithine 200-fold.
Reduces activity by 33%. Reduces affinity for ornithine 30-fold.
Reduces activity by 50%. Reduces affinity for ornithine 20-fold.
Reduces activity by 70%. Reduces affinity for ornithine 18-fold.
Reduces activity by 90%. Reduces affinity for ornithine 6-fold.
Reduces activity by 95%. Reduces affinity for ornithine 2-fold.
Abolishes interaction with BUB1 and BUB3 and spindle checkpoint function.
Spindle assembly checkpoint component MAD1
Histone H3-like centromeric protein CSE4
In CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with S-176.
In CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with T-218.
In CSE4-110; impairs nuclear division by disrupting the core centromere structure.
In CSE4-111; impairs nuclear division by disrupting the core centromere structure.
Meiosis-specific transcription factor NDT80
No effect on DNA-binding but strongly reduces progress through meiosis and sporulation.
Reduces DNA-binding by 50% and abolishes sporulation.
Reduces DNA-binding by 50% but does not abolish sporulation.
Reduces DNA-binding by 80% and abolishes sporulation.
Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation.
Reduces DNA-binding by 93% and abolishes sporulation.
Reduces DNA-binding by 95% and abolishes sporulation.
Reduces DNA-binding by 96% and abolishes sporulation.
Peptidyl-prolyl cis-trans isomerase CPR6
Abolishes iron-sulfur-binding; when associated with A-519; A-768 and A-771. Impaired nuclease and ATPase activities; when associated with A-771.
Abolishes iron-sulfur-binding; when associated with A-519; A-768 and A-777. Impaired nuclease and ATPase activities; when associated with A-777.
Abolishes iron-sulfur-binding; when associated with A-519; A-771 and A-777. Impaired nuclease and ATPase activities; when associated with A-519.
Abolishes iron-sulfur-binding; when associated with A-768; A-771 and A-777. Impaired nuclease and ATPase activities; when associated with A-768.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-17 and A-237.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-4 and A-17.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-4 and A-237.
DNA replication ATP-dependent helicase/nuclease DNA2
In dna2-1; temperature-sensitive mutant unable to grow at 37 degrees Celsius, probably due to abolition of iron-sulfur-binding.
Mimics phosphorylation; restores nuclear localization and recruitment at double-strand. break (DSB) sites following DNA damage; when associated with D-17.
Mimics phosphorylation; restores nuclear localization and recruitment at double-strand. break (DSB) sites following DNA damage; when associated with D-237.
Nuclease dead mutant. No helicase activity when the 5'-end of the substrate is blocked.
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Abolishes interaction with VPS4. Impairs sorting.
Loss of activity; important for dimerization.
In strain: CBS 1171 and in allele SED1-1, SED1-2, SED1-3, SED1-4, SED1-5, SED1-6 and SED1-7.
Influences the redox potential of the prosthetic heme and FAD groups
Involved in heme-bound ligand stabilization and O-O bond activation
Constitutive activity; when associated with R-451.
Loss of activity. Constitutive activity; when associated with R-224.
Uncharacterized RING finger protein YBR062C
Strongly diminishes the catalytic activity towards both known substrates, aconitate and homoaconitate.
Putative uncharacterized protein YAL064W
Prevents the release of AFG2, RLP24 and NOG1 from pre-60S ribosomal particles. Enhances growth defect in an AFG2 (drg1-18) mutant background.
Sufficient for interaction with MEX67 and KAP95
Mitochondrial inner membrane protein SHH3
Mitochondrial import inner membrane translocase subunit TIM21
Chitobiosyldiphosphodolichol beta-mannosyltransferase
Abolishes binding to SUI3; probably impairs the conversion of eIF-2-GDP to eIF-2-GTP.
Translation initiation factor eIF-2B subunit epsilon
Putative uncharacterized protein YLR157W-E
ATP synthase subunit beta, mitochondrial
Required for respiratory growth protein 8, mitochondrial
Meiosis-specific APC/C activator protein AMA1
Low affinity vacuolar monovalent cation/H(+) antiporter
Multifunctional tryptophan biosynthesis protein
Transcription initiation factor TFIID subunit 11
Ribonucleoside-diphosphate reductase large chain 2
RNA polymerase II-associated protein RBA50
Non-structural maintenance of chromosome element 3
Causes accumulation of pre-mRNA splicing intermediates.
Loss of activity. No effect on interaction with LCB2.
No effect on stability. Destabilizes LCB2.
No effect on stability. No effect on LCB2 stabilization.
No effect on stability. Partially stabilizes LCB2.
Required for interaction with FIS1 and MDV1
Abolishes ability to monoubiquitinate histone H2B.
Probable serine/threonine-protein kinase KKQ8
Phosphatidylinositol transfer protein PDR16
Decreases catalytic activity 2-fold, but retains substrate binding properties.
Geranylgeranyl transferase type-1 subunit beta
In cal1-1; completely abolishes geranylgeranyl transferase activity. Displays a defect in nuclear division and bud formation.
In cdc43-2; severely impairs the geranylgeranyl transferase activity.
In cdc43-3; severely impairs the geranylgeranyl transferase activity.
In cdc43-4,5,6; severely impairs the geranylgeranyl transferase activity.
In cdc43-7; severely impairs the geranylgeranyl transferase activity.
Increases KM for isoprenoid substrate 29-fold.
Increases KM for peptide substrate 12-fold.
5-glutamyl coenzyme A thioester intermediate
Serine/threonine-protein phosphatase 2B catalytic subunit A1
Carbamoyl-phosphate synthase arginine-specific small chain
In MRP21-1; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs.
In MRP21-3; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs.
Uncharacterized mitochondrial membrane protein FMP10
Abolished acetylation by ESA1, leading to decreased diacylglycerol synthesis; when associated with R-496.
Abolished acetylation by ESA1, leading to decreased diacylglycerol synthesis; when associated with R-801.
Impairs membrane recruitiment; when associated with A-11 and A-15.
Impairs membrane recruitiment; when associated with A-8 and A-11.
Impairs membrane recruitiment; when associated with A-8 and A-15.
Impairs phosphatidate phosphatase activity.
Vacuolar protein sorting-associated protein 16
Suppresses defects in the 5'UTLs of COX2 and COX3 mitochondrial mRNAs.
Binding site for the uncoating ATPase, involved in lattice disassembly
Involved in binding clathrin light chain
Causes progressive telomere shortening and cell senescence, but does not impair interaction with the telomerase RNP.
Causes progressive telomere shortening and cell senescence.
Causes telomere shortening, but does not impair interaction with the telomerase RNP.
Impairs interaction with the telomerase RNP and causes progressive telomere shortening and cell senescence.
Impairs interaction with the telomerase RNP and causes telomere shortening.
Reduces ability to dimerize and causes telomere shortening.
Reduces binding to DNA and RNA, and causes progressive telomere shortening and cell senescence.
Sphingolipid long chain base-responsive protein PIL1
Cells sensitive to calcofluor, caffeine and Congo red.
Abolished pseudouridylation of rRNA. Abolishes pseudouridylation at position 93 in U2 snRNA.
H/ACA ribonucleoprotein complex subunit CBF5
Reduced pseudouridylation of rRNA and reduced snoRNA levels.
Abolishes both palmitoylation and N-myristoylation.
Abolishes palmitoylation but not N-myristoylation.
Causes a specific mating defect in alpha cells.
Causes constitutive activation of the pheromone response pathway.
Enhances the rate of GDP for GTP exchange and slows hydrolysis of GTP, resulting in a constitutively active form that down-regulates the pheromone response independently of the pheromone receptor.
Forms a nondissociable complex with the pheromone receptor in response to receptor activation, resulting in reduced pheromone responsiveness.
Guanine nucleotide-binding protein alpha-1 subunit
Has increased GTP occupancy and moderately reduces hydrolysis of GTP, resulting in a constitutively active form that down-regulates the pheromone response and causes hyperadaptation to pheromone.
Impairs pheromone signaling in a and alpha cells.
Impairs pheromone signaling specifically in a cells.
In GPA1(SST); weakens interaction to SST2 and blocks its negative regulatory effect.
Leads to a hypersensitive signaling phenotype resulting in greatly enhanced signal at low alpha-factor concentrations.
Prevents GDP to GTP exchange; suppressor of L-323.
Prevents hydrolysis of GTP; eliminates the interaction with STE4 and constitutively activates the pheromone response pathway.
Reduces ligand-dependent pheromone signaling.
Slightly reduces ligand-dependent pheromone signaling.
Suppressor of L-323; does not prevent GTP binding to GPA1.
Abolishes substrate binding. Unable to confer thermotolerance. Reduces ATP hydrolysis by 98%; when associated with T-315. Completely abolishes ATPase activity; when associated with T-620.
Abolishes the ability to refold aggregated protein in vitro and to provide thermotolerance in vivo.
Almost completely abolishes ATP hydrolysis at NBD2, but does not affect nucleotide binding, thus keeping NBD2 in an ATP-bound state. Reduces stimulation of ATP hydrolysis upon substrate binding.
Can oligomerize in the absence of nucleotides.
Confers resistance to prion-curing by guanidine.
Impairs oligomerization at low protein concentrations.
Impairs oligomerization at low protein concentrations. Reduces ATP hydrolysis rate. Unable to confer thermotolerance. Completely abolishes ATPase activity; when associated with T-218.
Impairs prion propagation, but does not affect thermotolerance.
In HSP104(TRAP); completely abolishes ATP hydrolysis, but does not affect nucleotide binding, thus keeping HSP104 in an ATP-bound state; when associated with Q-285.
In HSP104(TRAP); completely abolishes ATP hydrolysis, but does not affect nucleotide binding, thus keeping HSP104 in an ATP-bound state; when associated with Q-687.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-778 and A-782.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-778 and A-789.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-782 and A-789.
Increases basal level of ATPase activity and abolishes stimulation of ATP hydrolysis upon substrate binding. Inhibits growth at 37 degrees Celsius.
Largely reduces ATP hydrolysis. Alters bud morphology and causes septin mislocalization; when associated with I-499.
Reduces ATP and ADP binding at NBD2 6-fold, but does not affect ATP hydrolysis at NBD2. Reduces catalytic rate at NBD1.
Reduces ATP hydrolysis by 50%. Alters bud morphology and causes septin mislocalization; when associated with S-217.
Reduces ATP hydrolysis by 98%; when associated with T-218.
Reduces ATP hydrolysis, but does not affect oligomerization.
Reduces ATPase activity by 80%. Impairs oligomerization.
Reduces rate of ATP hydrolysis at NBD1 nearly 10-fold. No effect on oligomerization.
Site-specific fluorescent probe in an otherwise Trp-less HSP104. Fluorescence of this Trp changes in response to ATP and ADP binding at NBD2. Has no effect on ATP hydrolysis or protein stability.
Topoisomerase I damage affected protein 8
Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with Ala-158.
Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with Ala-160.
Impairs MSH2-MSH3-mediated DNA mismatch repair.
Partially functional in a mismatch repair assay; when associated with 10-AA-11.
Partially functional in a mismatch repair assay; when associated with A-4.
Decreases gene expression from the ho locus.
In mkt1-19; HO gene expression is decreased.
Glycerol uptake/efflux facilitator protein
Regulator of V-ATPase in vacuolar membrane protein 1
Mitochondrial phosphate carrier protein 2
Rho1 guanine nucleotide exchange factor TUS1
Genetic interactor of prohibitins 3, mitochondrial
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
In DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with K-67.
In DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with V-157.
In DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with I-124.
In DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with T-117.
Guanine nucleotide-binding protein subunit beta
Chromosome transmission fidelity protein 18
Potential protein lysine methyltransferase SET6
In SSB1-D1: Reduces ribosome-binding to less than 50%.
In SSB1-D2: Reduces ribosome-binding to less than 50%.
In SSB1-L(BC): Reduces ribosome-binding to less than 50%.
Ribosome-associated molecular chaperone SSB1
Unable to hydrolyze ATP and moderately reduces ribosome binding.
Y' element ATP-dependent helicase protein 1 copy 3
Y' element ATP-dependent helicase protein 1 copy 7
Phospholipid-transporting ATPase accessory subunit CRF1
Mediator of replication checkpoint protein 1
Chromatin structure-remodeling complex protein RSC58
Failure to mediate ceramide transport; when associated with A-340.
Failure to mediate ceramide transport; when associated with A-472.
Probable alpha-1,6-mannosyltransferase MNN10
U3 small nucleolar RNA-associated protein 9
Changes the ribosome profile distribution of the protein and also increases its occurrence in the cytosolic fraction.
Changes the ribosome profile distribution of the protein.
Glycerophosphocholine phosphodiesterase GDE1
Membrane-anchored lipid-binding protein LAM6
U2 small nuclear ribonucleoprotein B""" /id="PRO_0000081895
Impairs interaction with COP1 and SEC21 and mislocalizes to the vacuole.
Impairs interaction with COP1, SEC21 and SEC23 and loss of reticulum endoplasmic exit.
Mediates the interactions with COPI and COPII coat complexes
Succinate dehydrogenase assembly factor 4, mitochondrial
In allele LHS1-1; constitutive ATPase activity.
Transcription initiation factor TFIID subunit 6
Cytochrome B pre-mRNA-processing protein 6
Altered inheritance of mitochondria protein 44
Complete loss of ability to support homology search.
DNA repair and recombination protein RAD54
38 X 8 AA approximate tandem repeats of S-[NS]-N-[ND]-D-S-Y-G
Conserved oligomeric Golgi complex subunit 8
Peptidyl-prolyl cis-trans isomerase C, mitochondrial
Strongly reduces in vitro PPIase activity and in vivo protein folding activity. Reduces binding to cyclosporin A 2-fold.
Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit.
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1063 and A-1282.
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1063 and A-1286.
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1282 and A-1286.
Does not affect elongator complex activity.
Does not affect elongator complex activity. Loss of elongator complex activity, reduced levels of mcm5U and ncm5U on tRNA and reduced interaction with HRR25 but no effect on elongator complex assembly; when associated with A-1198.
Does not affect elongator complex activity. Loss of elongator complex activity, reduced levels of mcm5U and ncm5U on tRNA and reduced interaction with HRR25 but no effect on elongator complex assembly; when associated with A-1202.
Loss of elongator complex activity. Abolishes binding to tRNA. Does not disrupt elongator complex assembly but decreases association of ELP1 with ELP5 and KTI12. Does not affect ELP1 self-association or association with ELP3. Does not affect the predominantly cytoplasmic localization.
Loss of phosphorylation at this site. Loss of elongator complex activity. Almost complete loss of mcm5U and ncm5U on tRNA. Does not affect elongator complex assembly. Enhances interaction with HRR25 and KTI12.
Phosphomimetic mutant. Does not affect elongator complex activity.
Slightly reduced levels of mcm5U and ncm5U on tRNA; when associated with E-1198.
Slightly reduced levels of mcm5U and ncm5U on tRNA; when associated with E-1202.
Some loss of elongator complex activity.
Myosin tail region-interacting protein MTI1
Cytochrome b-c1 complex subunit 1, mitochondrial
Restores water permeability; when associated with P-255.
Restores water permeability; when associated with V-121.
Geranylgeranyl pyrophosphate synthase BTS1
Important for determining product chain length
Monomer and homodimer. Monomer; when associated with G-7.
Mostly monomer. Exclusively monomer; when associated with G-8. Reduces enzyme activity 1000-fold.
No effect. Monomer; when associated with G-8.
Reduced affinity for isopentenyl diphosphate (IPP).
In TRF4-194; lethal; when associated with A-195; A-196 and A-198.
In TRF4-282; lethal; when associated with A-282.
In TRF4-282; lethal; when associated with A-285.
In TRF4-378; lethal; when associated with A-378.
In TRF4-378; lethal; when associated with A-381.
In TRF4-425; lethal; when associated with A-425.
In TRF4-425; lethal; when associated with A-428 and A-429.
In TRF4-502; lethal; when associated with A-502.
In TRF4-502; lethal; when associated with A-504.
In TRF4-559; slow growth; when associated with A-559.
Loss of interaction with SEC23/SEC24 complex.
Golgi membrane targeting and interaction with ARL1
Required for function in membrane trafficking
Amino-acid acetyltransferase, mitochondrial
Abolishes histone acetylase activity. Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Decreases activity stimulation by VPS75.
Decreases binding and activity stimulation by VPS75.
Decreases binding and activity stimulation by VPS75. Decreases acetylation of histone H3 'Lys-9' and 'Lys-27'.
Decreases histone acetylase activity. Decreases expression (at protein level). Increases sensitivity to methyl methane sulfonate and hydroxyurea (genotoxic stress).
Decreases histone acetylase activity. Increases sensitivity to methyl methane sulfonate and hydroxyurea (genotoxic stress).
Decreases histone acetylase activity; when associated with A-211.
Decreases histone acetylase activity; when associated with A-222.
Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Mildly increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Normal histone acetylase activity. Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Cytochrome c lysine N-methyltransferase 1
Negative cofactor 2 complex subunit alpha
ATP synthase subunit alpha, mitochondrial
Reduces level of VLP formation and maturation.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, H-210, D-364, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, D-364, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-371.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with V-186, H-210, D-364, G-371 and G-417.
Endoplasmic reticulum transmembrane protein 2
Abolishes aminopeptidase activity. No effect on the epoxide hydrolase activity.
Abolishes the aminopeptidase activity and decreases the epoxide hydrolase activity.
Abolishes the epoxide hydrolase activity.
Abolishes the epoxide hydrolase and aminopeptidase activities.
Increases the aminopeptidase activity and decreases the epoxide hydrolase activity.
Strongly reduces the substrate affinity.
Uncharacterized membrane protein YGR026W
Uncharacterized mitochondrial protein RF1
Loss of activity. No effect on interaction with LCB1.
V-type proton ATPase subunit c""" /id="PRO_0000071780
Regulator of V-ATPase in vacuolar membrane protein 2
Dolichyl-phosphate-mannose--protein mannosyltransferase 3
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Leads to increased stability and reduced response to degradation signals.
Leads to normal response to the FPP-derived signal but no response to the oxysterol signal.
7-keto 8-aminopelargonic acid transporter
KKRVK motif; Required for interaction with GLC7, endocytosis and actin cytoskeleton organization
Putative uncharacterized protein YPL278C
Chromatin structure-remodeling complex protein RSC30
Homoisocitrate dehydrogenase, mitochondrial
Vacuolar protein sorting-associated protein 51
DNA-directed RNA polymerase II subunit RPB9
Glyceraldehyde-3-phosphate dehydrogenase 2
Abrogates the introduction of ceramides into the GPI anchor.
Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium.
Impairs the introduction of ceramides into the GPI anchor.
No effect on introduction of ceramides into the GPI anchor.
Required for function in lipid remodeling
16 X 7 AA approximate repeats of T-G-G-A-M-M-P
Actin cytoskeleton-regulatory complex protein SLA1
Ubiquitin carboxyl-terminal hydrolase 12
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-119 and A-121.
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-119 and A-123.
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-121 and A-123.
Protein phosphatase 1 regulatory subunit SDS22
Phosphoribosylglycinamide formyltransferase
Phosphatidylinositol N-acetylglucosaminyltransferase ERI1 subunit
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
Postreplication repair E3 ubiquitin-protein ligase RAD18
Putative mitochondrial translation system component PET127
RNA polymerase I-specific transcription initiation factor RRN5
Loss of function, but still forms a heterodimer with SSZ1 and associates with ribosomes.
Eliminates glycogen accumulation; when associated with F-230 and F-232.
Eliminates glycogen accumulation; when associated with F-230 and F-367.
Eliminates glycogen accumulation; when associated with F-232 and F-367.
Transcriptional activator of sulfur metabolism MET28
Aspartate aminotransferase, mitochondrial
Abolishes ubiquitin-binding and vacuole sorting of ubiquitinated proteins.
Vacuolar protein-sorting-associated protein 36
Ankyrin repeat-containing protein YIL001W
Mitochondrial distribution and morphology protein 32
Proton acceptor; for 3-dehydroquinate dehydratase activity
Proton acceptor; for 3-dehydroquinate synthase activity
Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-68 and E-71.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-68 and E-73.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-71 and E-73.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-68; E-71 and E-73.
Strong decrease of the sporulation rate.
Gly-cisPro motif, important for rejection of L-amino acids
ER-localized J domain-containing protein 5
Abolishes interaction with TIF5; when associated with 16-K--K-23 and 49-K--K-56.
Abolishes interaction with TIF5; when associated with 16-K--K-23 and 82-K--K-89.
Abolishes interaction with TIF5; when associated with 49-K--K-56 and 82-K--K-89.
Eukaryotic translation initiation factor 2 subunit beta
Maintenance of telomere capping protein 2
Beta-glucan synthesis-associated protein SKN1
Abolishes kinase activity and termination by RNA polymerase I.
In wss1-pd; loss of function; when associated with A-115.
In wss1-pd; loss of function; when associated with A-119.
Required and sufficient for binding to DNA
Putative aryl-alcohol dehydrogenase AAD15
Mediator of RNA polymerase II transcription subunit 10
Auxilin-like clathrin uncoating factor SWA2
In SWA2-1/SWA2-TPR; partial loss of clathrin disassembly function. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with 631-AAA-633.
In SWA2-J; abolishes ATPase stimulation activity. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with R-388.
Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating
Serine/threonine-protein phosphatase PP2A-like PPG1
Putative uncharacterized protein YGL262W
ATPase expression protein 1, mitochondrial
Disrupts interaction with HRT1 and prevents RUB1/NEDD8 modification.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)
Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Bypasses the requirement for phosphatase YVH1 for the release of MRT4.
Transcription initiation factor IIA large subunit
2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
Required for function but dispensable for interaction with DPH2 and DPH3
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-239.
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-368.
Uncharacterized glycosyl hydrolase YBR056W
Regulator of Ty1 transposition protein 105
Leads to complete loss of association with ub-hotspots; when associated with A-33.
Leads to complete loss of association with ub-hotspots; when associated with A-34.
Strongly reduces EUC1 sumoylation and the formation of ub-hotspots.
Serine/threonine-protein phosphatase PP-Z1
RNA 3'-terminal phosphate cyclase-like protein
In RSM28-1; allows translation of defective COX2 mRNAs.
E3 ubiquitin-protein ligase substrate receptor MMS22
Putative transferase CAF17, mitochondrial
Uncharacterized SVF1-like protein YLR225C
Induces impairment in folding and loss of zinc-binding.
Interaction with transmembrane regions of transmembrane proteins in transit
Loss of function due to severely affected folding and the presence of non-native disulfide bonds; loss of zinc-binding.
Mitochondrial import inner membrane translocase subunit TIM10
Cullin-associated NEDD8-dissociated protein 1 homolog
Nicotinamide mononucleotide adenylyltransferase
Sodium/potassium exporting P-type ATPase 5
Vacuolar protein sorting-associated protein 73
Abolished binding to phosphatidylserine.
Does not affect binding to phosphatidylserine.
Strongly reduced binding to phosphatidylinositol 4-phosphate (PI4P). Does not affect binding to phosphatidylserine.
Strongly reduced binding to phosphatidylserine and phosphatidylinositol 4-phosphate (PI4P).
Strongly reduced binding to phosphatidylserine.
Alpha N-terminal protein methyltransferase 1
Mitochondrial import receptor subunit TOM7
Heterotrimeric G protein gamma subunit GPG1
Abolishes the interaction with FKH1; when associated with A-776.
Abolishes the interaction with FKH1; when associated with A-785.
Reduces the interaction with FKH1; when associated with A-776.
Reduces the interaction with FKH1; when associated with A-785.
LETM1 domain-containing protein YLH47, mitochondrial
DSC E3 ubiquitin ligase complex subunit 2
Mitochondrial import receptor subunit TOM5
Phosphate system positive regulatory protein PHO4
Eukaryotic translation initiation factor 5A-1
Impairs association to the ribosome and cell growth. Reduced ability to promote CAT tailing in response to ribosome stalling.
Impairs programmed ribosomal frameshifting.
In ts1159; temperature-sensitive growth phenotype and impairs programmed ribosomal frameshifting. Impaired ability to promote efficient translation of polyproline-containing peptides that stall ribosomes.
Lethal. Reduced binding to the ribosome; when associated with H-22.
Lethal; when associated with D-118 and D-124.
Lethal; when associated with D-118 and N-120.
Reduced ability to promote CAT tailing in response to ribosome stalling.
Temperature-sensitive growth phenotype. Impaired ability to promote CAT tailing in response to ribosome stalling.
Temperature-sensitive growth phenotype. Lethal; when associated with L-83 and D-118 or with I-66 and D-118 or with D-118 and I-142 or with L-116 and D-118.
Temperature-sensitive growth phenotype. Lethal; when associated with Y-39 and D-118.
Temperature-sensitive growth phenotype. Reduced binding to the ribosome.
Temperature-sensitive growth phenotype; when associated with D-118. Lethal; when associated with Y-39 and D-118.
Temperature-sensitive growth phenotype; when associated with F-93. Reduced binding to the ribosome; when associated with F-93.
Temperature-sensitive growth phenotype; when associated with L-116 or W-122 or F-140 or I-142. Lethal; when associated with Y-39 and I-66 or with Y-39 and L-83 or with Y-39 and L-116 or with Y-39 and F-140 or with Y-39 and I-142 or with N-120 and D-124.
Temperature-sensitive growth phenotype; when associated with V-118.
Temperature-sensitive growth phenotype; when associated with W-122.
Dihydrolipoyl dehydrogenase, mitochondrial
Affects protein stability, but has no effect on peptidase activity.
Temperature sensitive; abolishes proteolytic activity for RIP1, but not for COX4.
No cell growth and 2-fold decrease in ATPase activity in vitro.
No cell growth and drastic decrease in ATPase activity in vitro.
N-terminal acetyltransferase A complex subunit NAT1
Ubiquitin carboxyl-terminal hydrolase 15
In THI6-1; both enzyme activities greatly decreased.
In THI6-2; both enzyme activities greatly decreased.
In THI6-3; possesses TMP-PPASE activity only.
Proton acceptor; for hydroxyethylthiazole kinase activity
Thiamine biosynthetic bifunctional enzyme
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
In ALG3-1; leads to an underglycosylation of secretory proteins.
Probable vacuolar amino acid transporter YPQ2
NADP-dependent 3-hydroxy acid dehydrogenase
Abolishes interaction with VTA1 and reduces endosomal localization.
Vacuolar protein-sorting-associated protein 60
Helical; Signal-anchor for type III membrane protein
Suppressor of lethality of KEX2 GAS1 double null mutant protein 1
General negative regulator of transcription subunit 1
Hypoxanthine-guanine phosphoribosyltransferase
Mildly decreases methylation of the 2'-O-ribose of cytidine at position 32 of the tRNA anticodon loop of tRNA(Phe).
Severely decreases methylation of the 2'-O-ribose of cytidine at position 32 of the tRNA anticodon loop of tRNA(Phe).
tRNA (32-2'-O)-methyltransferase regulator TRM732
Low molecular weight phosphotyrosine protein phosphatase
No phosphorylation; when associated with A-319.
No phosphorylation; when associated with A-320.
No phosphorylation; when associated with A-322.
No phosphorylation; when associated with A-323.
Sensitive to alkali. Fails to restore normal levels of SLT2 phosphorylation upon alkaline stress.
Abolished fumarate hydratase activity and ability to participate in DNA repair.
Abolishes processing by the mitochondrial processing peptidase. Mainly localizes in the cytosol, with a small fraction in the mitochondrion. Reduced fumarate hydratase activity.
Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.
Mitochondrial import inner membrane translocase subunit TIM23
Trafficking protein particle complex subunit 20
High-affinity nicotinic acid transporter
CDP-diacylglycerol--serine O-phosphatidyltransferase
In SRP1-1; temperature-sensitive mutant.
In SRP1-2; temperature-sensitive mutant.
In SRP1-31; temperature-sensitive mutant; reduced growth rate and chromosome loss.
In SRP1-3; temperature-sensitive mutant.
In SRP1-49; temperature-sensitive mutant; alteration in nucleolar and microtubule morphology.
In SRP1-54; temperature-sensitive mutant; reduced growth rate.
Sporulation-specific with a leucine zipper motif protein 1
Serine/threonine-protein phosphatase PP-Z2
Ethanolamine-phosphate cytidylyltransferase
Putative aryl-alcohol dehydrogenase AAD10
In mutant W7 which is respiratory deficient.
Altered inheritance of mitochondria protein 19, mitochondrial
In strain: CLIB 556 haplotype Ha1 and CLIB 630.
Regulator of rDNA transcription protein 13
Uncharacterized membrane protein YJL163C
Catalytically inactive. Internalization defects.
No kinase activity. ATP-binding defect. Internalization defects.
No phosphorylation. Reduced sphingolipid biosynthesis inhibitor drug myriocin (ISP-1) resistance. No defect in growth or endocytosis.
No phosphorylation; when associated with A-504 or D-504.
No phosphorylation; when associated with A-662 or D-662.
Reduced sphingolipid biosynthesis inhibitor drug myriocin (ISP-1) resistance. Defective in growth and endocytosis.
70% reduction in kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure.
Abolishes the stationary phase-dependent stimulation of DAG kinase activity.
Loss of kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure.
No kinase activity. Not temperature-sensitive for growth. Does not trigger nuclear membrane expansion.
Pyridoxal 5'-phosphate synthase subunit SNO1
Vacuolar protein-sorting-associated protein 24
Cannot restore cell growth in USB1 depleted cells.
Does not affect growth phenotype. Significantly decreases in vitro processing.
Histidine biosynthesis trifunctional protein
Chromatin modification-related protein EAF7
Temperature-sensitive. Reduced sporulation efficiency.
Temperature-sensitive; when associated with L-806. Altered cell cycle distribution.
Temperature-sensitive; when associated with M-881. Altered cell cycle distribution.
Malonyl CoA-acyl carrier protein transacylase, mitochondrial
Uncharacterized endoplasmic reticulum membrane protein YNL046W
Putative uncharacterized protein YPR202W
Decreases transport activity and GDP-mannose-binding.
In VIG4-1; increases drug sensitivity and decreases protein glycolysis.
In VIG4-2; increases drug sensitivity and decreases protein glycolysis.
Uncharacterized transcriptional regulatory protein YNR063W
Inositol phosphosphingolipids phospholipase C
Serine/threonine-protein kinase HAL4/SAT4
Biogenesis of lysosome-related organelles complex 1 subunit KXD1
Branched-chain-amino-acid aminotransferase, cytosolic
Putative mitochondrial carnitine O-acetyltransferase
Abolishes rapamycin-resistance of mutants E-1972; I-1972 and R-1972.
Confers resistance to rapamycin. Abolishes interaction with FKBP-rapamycin.
In DRR1-1; confers resistance to rapamycin. Abolishes interaction with FKBP-rapamycin.
In DRR1-27; confers resistance to rapamycin.
Sensitive to high hydrostatic pressure (mechanical stress).
In PBS2-13; loss of SH3-domain interaction.
Putative uncharacterized protein YBR285W
Mitochondrial fusion and transport protein UGO1
Abolishes FBP1 ubiquitination and degradation.
Impaired ability to grow on nonfermentable carbon sources.
Inability to grow on nonfermentable carbon sources.
Ubiquinone biosynthesis protein COQ9, mitochondrial
Decreases glucosylceramide and phosphatidylcholine import into cells.
Decreases phosphatidylcholine import into cells, but does not apparently affect glucosylceramide transport.
Loss of activity; when associated with A-386; A-396; A-1566 and A-1592.
Loss of activity; when associated with A-386; A-396; A-403 and A-1566.
Loss of activity; when associated with A-386; A-396; A-403 and A-1592.
Loss of activity; when associated with A-386; A-403; A-1566 and A-1592.
Loss of activity; when associated with A-396; A-403; A-1566 and A-1592.
No apparent gain of activity; when associated with D-386; D-396; D-1566 and D-1592.
No apparent gain of activity; when associated with D-386; D-396; D-403 and D-1566.
No apparent gain of activity; when associated with D-386; D-396; D-403 and D-1592.
No apparent gain of activity; when associated with D-386; D-403; D-1566 and D-1592.
No apparent gain of activity; when associated with D-396; D-403; D-1566 and D-1592.
Transcription activator of gluconeogenesis ERT1
Probable pseudouridine-5'-phosphatase YKL033W-A
Cysteine-rich and transmembrane domain-containing protein YDL012C
Putative uncharacterized protein YGR079W
Abolished kinase activity. Loss of cell viability under starvation.
Enables the binding of the inhibitor of the kinase activity 1-NA-PP1.
Impairs autophagic activity; when associated with A-508.
Impairs autophagic activity; when associated with A-515.
Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-429.
Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-432.
Leads to constitutive autophosphorylation activity.
Loss of cell viability under starvation.
ATPase expression protein 2, mitochondrial
Suppresses a T-to-C nucleotide transition mutation in the 5' untranslated region of OLI1 mRNA localized 16 nucleotides upstream of the OLI1 reading frame.
Loss of catalytic activity. Loss of ATP binding. Reduced survival and growth in response to reactive oxygen species.
Protein adenylyltransferase SelO, mitochondrial
Essential for maintenance of the cell wall protein 1
DNA replication complex GINS protein PSF2
Cytochrome c oxidase assembly protein COX19
In COX19-1; causes a respiratory-deficient phenotype because of lack of cytochrome c activity.
Abolishes its physical interaction with NEO1. Suppresses the growth defects caused by mutations of flippases.
Suppresses the growth defects caused by mutations of flippases.
Hexaprenyl pyrophosphate synthase, mitochondrial
Nascent polypeptide-associated complex subunit beta-1
Increased microtubule polymerization and depolymerization rates. Increased microtubule stability. Decreased kinesin KIP3 subcellular location at microtubule plus ends.
Uncharacterized mitochondrial carrier YPR011C
Chromosome segregation in meiosis protein 3
RNA polymerase II subunit A C-terminal domain phosphatase SSU72
Guanine nucleotide-exchange factor SEC12
Protein phosphatase PP2A regulatory subunit A
Serine/threonine-protein kinase PTK2/STK2
Cytochrome c oxidase subunit 5B, mitochondrial
Probable NADPH:adrenodoxin oxidoreductase, mitochondrial
Reduces enzymatic activities by almost 95%.
Disrupts interaction with POL1 and FUN12.
Disrupts interaction with POL1 but not FUN12.
Increase in length of X' and Y' telomeres. Disrupts interaction with POL1 but not FUN12.
Constitutively in the GDP-bound conformation. Causes loss of function during vacuolar morphogenesis.
Constitutively in the GTP-bound conformation. Complements the fragmented vacuolar morphology of ypt7 null mutant cells.
Peroxisomal membrane protein import receptor PEX19
Mitochondrial intermembrane space cysteine motif-containing protein MIX17
Charge relay system; for autoendoproteolytic cleavage activity
Cleavage (non-hydrolytic); by autocatalysis
Enables targeting to the endoplasmic reticulum in addition to mitochondria
Impairs processing of the proenzyme and increases protein degradation. Decreases activity.
Impairs processing of the proenzyme. Decreases activity.
In PSD1deltaIM; Mislocalizes to the mitochondrial matrix.
Increases protein degradation. Decreases activity.
Loss of autocatalytic processing and leads to protein degradation. Complete loss of activity. Growth on the fermentable carbon source galactose is moderately decreased.
Loss of autocatalytic processing and leads to protein degradation. Complete loss of activity. Growth on the fermentable carbon source galactose is severely decreased.
Mitochondrion; not cleaved when targeted to the endoplasmic reticulum
No processing of the proenzyme, complete loss of activity.
No processing of the proenzyme, complete loss of activity. Increases protein localization to the endoplasmic reticulum; when associated with A-210 and A-345.
No processing of the proenzyme. Increases protein localization to the endoplasmic reticulum; when associated with A-210 and A-463.
No processing of the proenzyme. Increases protein localization to the endoplasmic reticulum; when associated with A-345 and A-463.
Phosphatidylserine decarboxylase 1 alpha chain
Phosphatidylserine decarboxylase 1 beta chain
Phosphatidylserine decarboxylase proenzyme 1, mitochondrial
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
Significantly impairs processing of the proenzyme, retains some activity.
In PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino acids.
In PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino acids.
Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1.
Serine/arginine (SR)-type shuttling mRNA binding protein HRB1
Uncharacterized protein YGL041W-A, mitochondrial
3',5'-cyclic-nucleotide phosphodiesterase 1
Lethal(2) giant larvae protein homolog SRO77
Strongly reduces UBC13 binding and interferes with error-free DNA repair.
Ubiquitin-conjugating enzyme variant MMS2
Mutants do not survive after treatment with DNA-damaging agent MMS.
Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutants show a slight growth defect after treatment with DNA-damaging agent MMS.
Mutants show a strong growth defect after treatment with DNA-damaging agent MMS.
Mutants show a very strong growth defect after treatment with DNA-damaging agent MMS.
Mutants show no effect after treatment with DNA-damaging agent MMS.
Probable electron transfer flavoprotein subunit alpha, mitochondrial
Low-affinity phosphate transporter PHO90
Mitochondrial 2-oxodicarboxylate carrier 1
General transcriptional corepressor TUP1
DNA polymerase alpha-associated DNA helicase A
Inosine-5'-monophosphate dehydrogenase 4
Reduces MCM2-7 complex helicase activity. Abolishes MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM3 A-415.
In the cold-sensitive allele sen2-3; defective in cleavage only at the 5'-splice site of tRNA precursors.
Serine/threonine-protein phosphatase PP2A-2 catalytic subunit
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Suppressor of hydroxyurea sensitivity protein 2
Probable phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TEP1
Chromatin structure-remodeling complex subunit SFH1
Destabilizes; not detectable in anaphase cells; spindle becomes thin and fragile.
In SEC2-78; temperature-sensitive; causes mislocalization of the protein, displaying many cytoplasmic punctae.
Rab guanine nucleotide exchange factor SEC2
Required for proper polarized localization of the protein
Strongly reduces interaction with HSP70 family members.
In VTI1-11; displays a block in traffic to the PVC and an additional defect in retrograde traffic to the cis-Golgi; when associated with F-155.
In VTI1-11; displays a block in traffic to the PVC and an additional defect in retrograde traffic to the cis-Golgi; when associated with G-145.
In VTI1-12; blocks constitutively the traffic from the late Golgi to the vacuole and blocks the transport to the cis-Golgi compartment at nonpermissive temperature; when associated with R-158.
In VTI1-12; blocks constitutively the traffic from the late Golgi to the vacuole and blocks the transport to the cis-Golgi compartment at nonpermissive temperature; when associated with S-141.
In VTI1-1; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with K-145.
In VTI1-1; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with R-148.
In VTI1-2; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with P-130.
In VTI1-2; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with T-151.
Uncharacterized membrane protein YAL064W-B
F(1)-ATPase inhibitor STF1, mitochondrial
Impairs RNA-binding (at the proposed ring entry site).
Impairs RNA-binding (at the proposed ring exit site).
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Probable glutathione-independent glyoxalase HSP33
General transcription and DNA repair factor IIH subunit TFB4
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Loss of phosphorylation. Greatly increases catalytic activity and promotes homodimerization.
Phosphomimetic mutant. Inhibits catalytic activity and homodimerization.
Spindle pole body-associated protein VIK1
Does not confer ability to stimulate the ATPase activity of mtHSP70.
Mitochondrial import inner membrane translocase subunit TIM16
ERAD-associated E3 ubiquitin-protein ligase ASI1
In ASI1-21HA; when associated with S-583; abolishes function.
In ASI1-21HA; when associated with S-585; abolishes function.
Putative uncharacterized protein YLL053C
Endoplasmic reticulum transmembrane protein 1
Transcription initiation factor TFIID subunit 12
Regulator of Ty1 transposition protein 103
Acetolactate synthase small subunit, mitochondrial
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial
In SEC14(ts); temperature-sesnitive allele that is targeted to the proteasome at the restrictive temperature.
Inactivates phosphatidylinositol, but not phosphatidylcholine, transfer activity, but rescues the lethality and Golgi secretory defects associated with sec14 null mutations; when associated with A-239.
Inactivates phosphatidylinositol, but not phosphatidylcholine, transfer activity, but rescues the lethality and Golgi secretory defects associated with sec14 null mutations; when associated with A-66.
Vacuolar protein sorting-associated protein 3
2-hydroxy-palmitic acid dioxygenase MPO1
Dominant negative mutant; displays respiratory defects that are higher than the BOL1 BOL3 double mutant.
Magnesium-activated aldehyde dehydrogenase, cytosolic
Uncharacterized membrane protein YDL180W
Stabilizes ubiquitin pathway substrates; when associated wirh Ala-109.
Stabilizes ubiquitin pathway substrates; when associated wirh Ala-111.
Ubiquitin carboxyl-terminal hydrolase RPN11
Impairs interaction with RAD3, reducing the interaction of TFIIH with the head module and consequently resulting in a reduction of RNA polymerase IICTD 'Ser-5' phosphorylation.
Impairs interaction with SRB4/MED17, SRB6/MED22 and RAD3.
Mediator of RNA polymerase II transcription subunit 11
Results in a decrease of TFIIK and RNA polymerase II occupancies at active promoters; when associated with K-17.
Results in a decrease of TFIIK and RNA polymerase II occupancies at active promoters; when associated with K-24.
Maintenance of telomere capping protein 4
GPI ethanolamine phosphate transferase 2
In GPI7-2; temperature-sensitive mutant that shows defects in cell separation and a daughter cell-specific growth defect.
Asymmetric dimethylarginine; by HMT1; alternate
Nuclear localization sequence-binding protein
Omega-N-methylarginine; by HMT1; alternate
Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial
Abolishes interaction with ORC1 and SIR4.
In cdc68-1; induces a spt phenotype characterized by depletion of many mRNAs.
In spt16-4; induces a spt phenotype characterized by depletion of many mRNAs; when associated with L-570.
In spt16-4; induces a spt phenotype characterized by depletion of many mRNAs; when associated with S-565.
In spt16-6; induces a spt phenotype characterized by depletion of many mRNAs.
In spt16-7; induces a spt phenotype characterized by depletion of many mRNAs.
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial
54S ribosomal protein L34, mitochondrial
Decreases phosphorylation by PKA. Leads to constitutive activation of autophagy; when associated with A-348; A-438; A-496; A-535; A-541; A-646 and A-649.
Impairs binding to ATG1; when associated with A-468.
Impairs binding to ATG1; when associated with A-469.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-541 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-541, and A-646.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-535; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-496; A-535; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-437; A-438; A-496; A-535; A-541; A-646 and A-649.
Eukaryotic translation initiation factor 3 subunit J
Increased association of the eIF-3 complex and 40S ribosomes.
Trafficking protein particle complex subunit 23
Repressor of RNA polymerase III transcription MAF1
t-SNARE affecting a late Golgi compartment protein 2
Impaired heterodimerization with ARP7; when associated with L-338.
Impaired heterodimerization with ARP7; when associated with V-337.
Results in nearly 50% loss of H3K4me3; when associated with K-180 and K-182.
Results in nearly 50% loss of H3K4me3; when associated with K-180 and K-186.
Results in nearly 50% loss of H3K4me3; when associated with K-182 and K-186.
Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.
Serine/threonine-protein phosphatase 4 regulatory subunit 2
ATP-dependent RNA helicase MSS116, mitochondrial
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
Uncharacterized membrane protein YGR016W
Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-304.
Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-397.
Abolishes phosphorylation; when associated with A-267; A-304; A-351 and A-397.
Abolishes phosphorylation; when associated with A-304; A-328; A-351 and A-397.
Anaphase-promoting complex subunit CDC27
In CDC27-633; G2/M cell cycle arrest at 35 degrees Celsius.
Cells accumulate 35S and 23S pre-rRNA precursors. Cells display defects in translation elongation resulting in decreased translational accuracy.
Histone deacetylase complex subunit CTI6
Impairs growth under iron-limiting conditions.
Completely abolishes catalytic activity.
Uncharacterized membrane protein YHL071W
Transcription initiation factor TFIID subunit 7
PAN2-PAN3 deadenylation complex subunit PAN3
Significantly decreases interaction with PAN1.
Appears to have no effect on the ATPase activity of membrane-detached V-ATPase V1 or on cell population growth.
Increases the ATPase activity of membrane-detached V-ATPase V1, appears to have no effect on cell population growth.
Reduced viability upon starvation protein 161
DNA-directed RNA polymerase I subunit RPA49
Loss of DNA binding; when associated with A-356.
Loss of DNA binding; when associated with A-358.
Alters protein substrate specificity. Able to farnesylate Ras2 variants with a negatively charged C-terminal aspartate (CIID).
Alters protein substrate specificity. Able to farnesylate Ras2 variants with positively charged C-terminal amino acids (CIIR and CIIK).
Can substitute for PGGT-I beta subunit (CDC43) in vivo. Has increased activity to farnesylate a substrate for PGGT-I. On the other hand, the ability to farnesylate its own substrate is reduced, due to its increased affinity for PGGT-I protein substrates. Increases the kcat/Km value for PGGTase-I substrates about 20-fold.
Can substitute for PGGT-I beta subunit, by modulating the substrate specificity for the peptide substrate.
Can substitute for PGGT-I beta subunit, by modulating the substrate specificity for the peptide substrate. Increases the kcat/Km value for PGGTase-I substrates about 20-fold.
Farnesylates Ras-CII(S,M,L) at wild type levels but can no longer geranylgeranylate the RasCII(M,L) substrates.
Farnesylates Ras-CIIS and Ras-CIIM but not Ras-CIIL, and is not capable of geranylgeranylating the Ras-CII(M,L) substrates.
Farnesylates only Ras-CIIS and not Ras-CII(M,L), and it geranylgeranylates all three substrates as well or better than wild type.
Important for selectivity against geranylgeranyl diphosphate
In ram1-1; causes a defect in a-factor production.
In ram1-2; abolishes farnesyltransferase activity.
Protein farnesyltransferase subunit beta
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
C-terminal peroxisome targeting signal (PTS1)
Putative uncharacterized protein YAR062W
Uroporphyrinogen-III C-methyltransferase
N-terminal acetyltransferase A complex catalytic subunit ARD1
U3 small nucleolar RNA-associated protein MPP10
Desensitizes cells to H(2)O(2) treatment.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Impairs ubiquitin conjugation, DNA double-strand breaks formation during meiosis and histone H3-K79 methylation.
Induces apoptotic-like features including chromatin condensation.
Acetylornithine aminotransferase, mitochondrial
Uncharacterized membrane protein YOR365C
High-affinity potassium transport protein
CRAL-TRIO domain-containing protein YKL091C
Chromatin structure-remodeling complex subunit RSC9
Chromatin structure-remodeling complex subunit RSC2
In dpm18; defective in plasmid maintenance.
In dpm3; defective in plasmid maintenance.
Reduces the Vmax 30-fold, but does not change the KM in a uridine hydrolase assay.
In DOC1-1; G2/M cell cycle arrest at 37 degrees Celsius.
Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85.
No phosphorylation by PKA, and strong decrease in activity; when associated with A-30.
Glycine dehydrogenase (decarboxylating), mitochondrial
Decreases JAC1 ATPase activator activity. Decreases vegetative cell population growth. Sensitive to high temperature.
Decreases JAC1 ATPase activator activity. Decreases vegetative cell population growth. Sensitive to high temperature. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; S-38 and L-41.
Decreases JAC1 ATPase activator activity; when associated with A-20; A-35 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; L-41 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-20; A-35 and S-38. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; S-38 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-20; S-38 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-20; S-38; L-41 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-35; S-38 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-35; S-38; L-41 and A-48.
In JAC1(LKDDEQ); impairs interaction with ISU1, but does not affect stimulation of SSQ1 ATPase activity.
In JAC1-1; temperature sensitive; causes growth defect at high temperatures.
In JAC1-A3; temperature sensitive; causes growth defect at high temperatures.
General transcription and DNA repair factor IIH subunit TFB5
Increases readthrough in translation termination.
Ribosome-associated complex subunit SSZ1
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
Mediator of RNA polymerase II transcription subunit 9
U3 small nucleolar RNA-associated protein 21
Cysteine sulfenic acid (-SOH) intermediate
ATPase-stabilizing factor 15 kDa protein
DNA polymerase alpha catalytic subunit A
Increase in length of X' and Y' telomeres. No effect on telomere position effect. Reduced interaction with CDC13.
Increase in length of X' and Y' telomeres. Reduced interaction with CDC13.
Serine/threonine-protein kinase/endoribonuclease IRE1
Protein phosphatase PP2A regulatory subunit B
Causes dissociation of the homotetramer to dimers at low concentrations.
Reduces kcat of the mutase reaction 10000-fold.
Ubiquitin carboxyl-terminal hydrolase YUH1
Phosphoribosylformylglycinamidine synthase
Impaired palmitoylation and plasma membrane localization.
Transcription initiation factor IIE subunit beta
Mitochondrial 2-oxodicarboxylate carrier 2
Causes mislocalization of chitin synthase CHS3 (PubMed:16818716). Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation (PubMed:26224664).
Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation.
Abolishes interaction with eIF4E; when associated with A-109.
Abolishes interaction with eIF4E; when associated with A-114.
May be involved in recognition of long-chain fatty acids in GPI
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrial
Guanine nucleotide-binding protein subunit beta 2
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-310.
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with R-104 or A-123.
In ytm1-1; abrogates binding to ERB1 and impairs 27S pre-rRNA processing and 66S pre-ribosome maturation; when associated with D-398.
In ytm1-1; abrogates binding to ERB1 and impairs 27S pre-rRNA processing and 66S pre-ribosome maturation; when associated with N-442.
Sufficient for interaction with ERB1 and association with 66S pre-ribosomes
Weakens the interaction with ERB1, leading to mostly ribosome-unbound YTM1 and defects in 60S maturation. Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-310.
Mitochondrial MRF1 N(5)-glutamine methyltransferase MTQ1
Nuclear distribution protein nudE homolog 1
Abolishes E3 activity, strongly reduces zinc binding and destabilizes the protein, but no effect on subcellular location.
Regulator of rDNA transcription protein 6
Probable alanine aminotransferase, mitochondrial
13 kDa ribonucleoprotein-associated protein
Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84.
Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81.
Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects.
Nuclear polyadenylated RNA-binding protein 3
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase
Pleckstrin homology (PH) domain conferring phosphoinositide binding specificity
DNA-directed RNA polymerase III subunit RPC8
In SPO1-1; defective in sporulation at 34 degrees Celsius.
Required for lipid-binding and function in meiosis
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51
AAA-ATPase; required for its chromatin boundary function
Increases YTA7 binding to the HTA1 open reading frame outside of S-phase. Increases localization of RTT106 and RSC8 to the HTA1 gene locus. Increases nucleosome occupancy at core histone loci. Decreases transcription of HTA1.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370 and A-380.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Deficiency in ubiquitin-protein conjugate formation.
Deficiency in ubiquitin-protein conjugate formation. Loss of DNA repair function.
Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69.
Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo.
Transcription initiation factor IIA subunit 2
Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27.
Transcription initiation factor IIF subunit alpha
Peptidyl-prolyl cis-trans isomerase FPR2
Putative aryl-alcohol dehydrogenase AAD14
U3 small nucleolar RNA-associated protein 11
Mitochondrial distribution and morphology protein 30
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
RNA polymerase II nuclear localization protein IWR1
Reduced kinase activity; when associated with A-641.
Reduced kinase activity; when associated with A-659.
Rescues growth of cells compromised in TORC2, but not TORC1 function. Restores SLT2 activation and suppresses actin cytoskeleton organization defect in TOR2 mutant cells.
Serine/threonine-protein kinase YPK2/YKR2
ADP-ribosylation factor GTPase-activating protein GLO3
Mediates translocation across the endoplasmic reticulum, renders the enzyme inactive during transit, and targets the molecule to the vacuole
N-linked (GlcNAc...) (high mannose) asparagine
Hydroxyacylglutathione hydrolase, cytoplasmic isozyme
Increased recombination centers protein 23
Putative guanine nucleotide-exchange factor SED4
Decreases the turnover number 145-fold. Decreases the turnover number 700-fold; when associated with Gln-96.
Decreases the turnover number 28-fold. Decreases the turnover number 700-fold; when associated with Met-77.
Saccharopine dehydrogenase [NAD(+), L-lysine-forming]
Locks VPS21 in its GDP-bound form by abolishing binding to GTP.
Vacuolar protein sorting-associated protein 21
In SRB2-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.
Mediator of RNA polymerase II transcription subunit 20
NEDD8-activating enzyme E1 catalytic subunit
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Silencing boundary-establishment protein FUB1
Altered inheritance of mitochondria protein 20
Putative uncharacterized protein YEL076C-A
Putative uncharacterized protein YLR464W
General transcriptional corepressor CYC8
Altered inheritance of mitochondria protein 6
Abolishes ATP binding, reducing ATPase activity by 95%. Displays 9800-fold increase in spontaneous mutation accumulation.
Abolishes heterodimer formation. Displays an increase in spontaneous mutation accumulation.
Defective in a mismatch repair assay. Abolishes heterodimer formation. Displays an increases spontaneous mutation accumulation.
Displays an increase in spontaneous mutation accumulation. Does not impair heterodimer formation.
In strain: EAY1066, EAY1068, M2-8, M7-8, M5-7, SK1, YJM145, YJM269, YJM320, YJM339 and YJM627; forms a non-functional heterodimer with PMS1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains.
Reduces ATPase activity by 98%. Displays 3300-fold increase in spontaneous mutation accumulation.
Reduces DNA-binding and displays a 1700-fold increase in spontaneous mutation accumulation. Strongly reduces DNA-binding and displays 12000-fold increase in spontaneous mutation accumulation; when associated with E-273.
Reduces heterodimer formation. Displays an weak increase in spontaneous mutation accumulation.
Strongly reduces DNA-binding and displays 12000-fold increase in spontaneous mutation accumulation; when associated with E-274.
Maintenance of telomere capping protein 5
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial
tRNA N6-adenosine threonylcarbamoyltransferase
Decreases DNF1 activity. Reduces interaction with DNF1. Resistant to miltefosine. Sensitive to duramycin.
Increases glucosylceramide transport activity of DNF1 and DNF2, but not their phosphatidylethanolamine or phosphatidylcholine transport activity.
Mildly decreases DNF1 activity. Reduces interaction with DNF1. Resistant to miltefosine.
Phospholipid-transporting ATPase accessory subunit LEM3
Required for localization to the plasma membrane
Strongly reduces interaction with DNF1. Mildly resistant to miltefosine. Decreases protein level. Normal protein level; when associated with C-110.
Strongly reduces interaction with DNF1. Mildly resistant to miltefosine. Decreases protein level. Normal protein level; when associated with C-159.
Beta-1,6-glucan synthesis-associated protein KEG1
In KEG1-1; temperature-sensitive. Increased sensitivity to chitin-binding dye Calcofluor white and zymolyase. Shows K1 killer toxin resistance, and reduced content of the cell wall beta-1,6-glucan.
Mitochondrial aspartate-glutamate transporter AGC1
Abolishes quinone reductase activity. Little effect on complex assembly.
Impairs respiratory growth and reduces quinone reductase activity.
Reduces SDHFAD content. Probably impairs complex assembly.
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Putative uncharacterized protein YAR069C
Mitochondrial import inner membrane translocase subunit TIM18
Association with SNF1 kinase complex (ASC) domain; required for interaction with SNF4
Changes protein distribution from the plasma membrane to the cytoplasm and nucleus and alters the cellular life span.
Kinase-interacting sequence (KIS); required for interaction with SNF1
Putative uncharacterized protein YLR125W
Impairs maturation and amino acid-induced STP1 cleavage.
In SSY5-13; constitutively active, confers 9.3% increased STP1 processing in the absence of amino acids.
In SSY5-14; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
In SSY5-15; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
In SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino acids; when associated with H-632.
In SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino acids; when associated with N-581.
In SSY5-6; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
Prevents maturation and amino-acid-induced STP1 cleavage.
SPS-sensor serine protease component SSY5
Abolishes ATPase activity, leading to secretion of resident ER proteins.
Prevents dimerization; when associated with S-285.
Prevents dimerization; when associated with S-288.
In pcl1-2A; stabilizes PCL1; when associated with A-39.
In pcl1-2A; stabilizes PCL1; when associated with A-43.
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI15
0.1% of catalytic activity. No specific ARS1 binding.
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
Impairs phosphorylation; when associated with A-119; A-149 and A-178.
Impairs phosphorylation; when associated with A-119; A-149 and A-248.
Impairs phosphorylation; when associated with A-119; A-178 and A-248.
Impairs phosphorylation; when associated with A-149; A-178 and A-248.
Stabilizes VAC17 whose protein levels are about 10-fold higher than wild-type.
Nuclear polyadenylated RNA-binding protein NAB2
Uncharacterized GTP-binding protein YGR210C
Sodium/potassium exporting P-type ATPase 2
Biogenesis of lysosome-related organelles complex 1 subunit SNN1
Diphosphoinositol polyphosphate phosphohydrolase DDP1
Altered inheritance of mitochondria protein 18, mitochondrial
Mitochondrial ATPase complex subunit ATP10
No interaction with FIS1; slight decrease in interaction with DNM1.
Results in mitochondrial division defect, but has no effect on the interaction with DNM1.
Slight decrease in interaction with DNM1.
Slight decrease in interaction with FIS1; 20-fold decrease in interaction with DNM1; even distribution along mitochondria not punctate.
Suppresses the mitochondrial fission defect of a FIS1-3 mutant by affecting interaction with FIS1.
ATP-dependent RNA helicase SUV3, mitochondrial
In suv3-1; probably excised introns are less efficiently released.
Uncharacterized ABC transporter ATP-binding protein/permease YOL075C
Abolishes endonuclease activity. In box3-2 /G1909; abolishes maturase and endonuclease activity; when associated with F-364.
Abolishes endonuclease activity. In box3-2 /G1909; abolishes maturase and endonuclease activity; when associated with N-393.
Abolishes maturase and endonuclease activity.
In M1282 / M4111; abolishes maturase and endonuclease activity.
In M4962; abolishes maturase and endonuclease activity.
In W404-3; abolishes maturase and endonuclease activity; when associated with M-395.
In W404-4; abolishes maturase and endonuclease activity; when associated with Y-379.
DNA repair and recombination protein RAD26
Uncharacterized transcriptional regulatory protein YLL054C
Protein phosphatase type 2A regulatory subunit RTS3
Mitotic spindle checkpoint component MAD2
No acetylation, reduced silencing activity.
In MNA6-1; causes temperature-dependent loss of the 15S rRNA.
In MNA6-2; causes temperature-dependent loss of the 15S rRNA.
In MNA6-3; causes temperature-dependent loss of the 15S rRNA.
In MNA6-4; causes temperature-dependent loss of the 15S rRNA.
In NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation.
Fails to reduce intracellular dinucleoside polyphosphate levels.
Decreases phosphatidic acid binding and impairs cardiolipin biosynthesis; when associated with A-62.
Decreases phosphatidic acid binding and impairs cardiolipin biosynthesis; when associated with A-65.
Decreases phosphatidic acid transfer activity and impairs cardiolipin biosynthesis.
Failure to complement the mitochondrial defects of UPS1-deficient cells.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-104.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-144.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-148 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-61 and E-148.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-61 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-61; E-148 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-58; E-61; E-148 and E-155.
Impairs interaction with MDM35. Reduces ability to complement the mitochondrial defects of UPS1-deficient cells.
Nearly abolishes phosphatidic acid transfer activity.
Nearly abolishes phosphatidic acid transfer activity; when associated with E-155.
Nearly abolishes phosphatidic acid transfer activity; when associated with E-61.
No effect on phosphatidic acid transfer activity.
Strongly impairs interaction with MDM35. Failure to complement the mitochondrial defects of UPS1-deficient cells.
Interferes with the hydrolysis of the covalent intermediate with DNA that is formed during the enzyme reaction. No effect on bleomycin sensitivity.
Strongly reduced release of the covalent intermediate with DNA that is formed during the enzyme reaction, leading to the accumulation of toxic adducts. No effect on bleomycin sensitivity.
Low residual phosphatase activity. Loss of stress response functions.
Abolishes cleavage by ESP1; when associated with D-268.
Abolishes first cleavage by ESP1. Abolishes all cleavage by ESP1; when associated with D-180.
Reduces phosphorylation. Abolishes phosphorylation; when associated with A-175.
Reduces phosphorylation. Abolishes phosphorylation; when associated with A-263.
Late endosome and vacuole interface protein 11
Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with A-158.
Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with Q-157.
Probable ATP-dependent RNA helicase DHR1
Probable target of rapamycin complex 2 subunit BIT2
Uncharacterized membrane protein YOL019W
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI11
Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-182.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-185.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-182 and A-185.
Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. Disrupts interaction with TUP1.
Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6.
Succinate dehydrogenase assembly factor 3, mitochondrial
Tethering factor for nuclear proteasome STS1
Respiratory supercomplex factor 2, mitochondrial
U3 small nucleolar RNA-associated protein 7
Dihydroxy-acid dehydratase, mitochondrial
Alters protein folding. Severely impaired growth. Strongly reduced growth at 35 degrees Celsius; when associated with A-1854.
Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius.
Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius. Strongly reduced growth at 35 degrees Celsius; when associated with A-1853.
Restriction endonuclease homology domain
Severely impaired growth. Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius.
Uncharacterized vacuolar protein YLR297W
Disrupts the heterotetrameric state and becomes hypersensitive to MMS; when associated with A-55 or 78-AA-79.
Disrupts the heterotetrameric state and becomes hypersensitive to MMS; when associated with A-62.
Probable serine/threonine-protein kinase KCC4
Asparagine synthetase domain-containing protein YML096W
Phosphatidylinositol transfer protein PDR17
Diminishes phosphorylation; 75-80% loss in kinase activity; no effect on survival.
No effect on phosphorylation; no effect on kinase activity.
EH domain-containing and endocytosis protein 1
In COQ4-1; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
In COQ4-2; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
In COQ4-3; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
Ubiquinone biosynthesis protein COQ4, mitochondrial
PXIXIT-like, required for interaction with CNA1 and CNA2, and calcineurin-dependent dephosphorylation
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM2
Required and sufficient for inner nuclear membrane localization
tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial
Putative uncharacterized protein YDL057W
Meiotic sister chromatid recombination protein 1
1-phosphatidylinositol 3-phosphate 5-kinase FAB1
Cells are defective for growth at 38 degrees Celsius and show single-lobed, enlarged vacuoles.
Cells are defective for growth at 38 degrees Celsius.
Cells are defective for growth at 38 degrees Celsius. PtdIns(3,5)P2 levels are severely reduced.
Cells show growth at 38 degrees Celsius. Failure to localize to the vacuole membrane.
Impairs intramolecular interaction with the CCR domain, leading to a hyperactive kinase mutant.
Impairs intramolecular interaction with the CCR domain, leading to a hyperactive kinase mutant; elevates PI(3,5)P2 levels.
Impairs intramolecular interaction with the kinase domain. Leads to elevated PI(3,5)P2 levels.
Loss of interaction with VAC14. Failure to localize to the vacuole membrane.
Required for localization to the vacuole membrane
Putative uncharacterized protein YMR321C
Ferric reductase transmembrane component 5
Major facilitator superfamily domain-containing protein YCR023C
Abolishes adenylyltransferase activity but not sulfurtransferase activity.
Abolishes sulfurtransferase activity but not adenylyltransferase activity.
Adenylyltransferase and sulfurtransferase UBA4
Cysteine persulfide intermediate; for sulfurtransferase activity
Glycyl thioester intermediate; for adenylyltransferase activity
Proton acceptor; for methoxycarbonylation activity
Proton donor; for both methylation and methoxycarbonylation activities
DNA-directed RNA polymerase I subunit RPA14
Fails to release from the spliceosome; when associated with D-349.
Fails to release from the spliceosome; when associated with H-548.
Has small amount of ATPase activity, but no splicing activity.
In PRP2-dn1; 40% of wild-type RNA-stimulated ATPase activity; splicing activity abolished; accumulates stalled splicing complexes.
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
Spliceosome binding mutant; not active in splicing; when associated with N-551, or D-349 and H-548. Loss of interaction with SPP2.
Spliceosome binding mutant; not active in splicing; when associated with N-551.
Spliceosome binding mutant; not active in splicing; when associated with N-551. Almost wild-type RNA-dependent ATPase activity.
Temperature-sensitive; decreased interaction with SPP2, decreased cell growth on benomyl and decreased splicing at elevated temperatures; when associated with D-349 and H-548.
Wild-type RNA-dependent ATPase activity; bound tightly to the spliceosome and after addition of ATP released from the spliceosome.
Digitonin sensitive, reduces structural integrity of the protein.
Digitonin sensitive, reduces structural integrity of the protein. Affects the substrate specificity.
Digitonin sensitive, reduces structural integrity of the protein. Greatly inhibits the fatty acid elongation cycle. Displays a higher KM and lower Vmax values.
Exhibits a moderate fatty acid elongation defect.
Greatly inhibits the fatty acid elongation cycle. Displays normal KM but reduced Vmax values.
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1
Abolishes self-association; when associated with G-332.
Abolishes self-association; when associated with L-335.
In mcm10-1; diminishes interaction with MCM7.
In mcm10-43; abolishes self-association and diminishes interaction with MCM7.
Inhibits interaction with POL30/PCNA and abolishes cell proliferation.
Temperature sensitive; loss of CDC17 stabilization.
Monoisotopic mass with either 6 methylation modifications or 1 acetylation and 3 methylation modifications.
25S rRNA (uridine(2634)-N(3))-methyltransferase
Vacuolar protein sorting-associated protein 71
Abolishes checkpoint function and interaction with MAD2, MAD3 and CDC20. Benomyl-sensitive phenotype.
Abolishes checkpoint function. Benomyl-sensitive phenotype.
Abolishes checkpoint function. Lowers interaction with BUB1 and MAD3. Benomyl-sensitive phenotype.
Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype.
Abolishes checkpoint function. No effect on interaction with BUB1. Lowers interaction with MAD3. Benomyl-sensitive phenotype.
Induces dysregulation of sphingolipid synthesis; when associated with A-15, A-18, A-36 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18, and A-36.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-36 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-18, A-36 and 46-A--A-48.
Mediates transcriptional repression, self-association and interaction with HIR2
Probable glutathione-independent glyoxalase HSP32
Impairs interaction with ATG5and autophagy.
Significantly reduces autophagic activity; when associated with A-101.
Significantly reduces autophagic activity; when associated with A-102.
Significantly reduces autophagic activity; when associated with A-104 and A-108.
Significantly reduces autophagic activity; when associated with A-104 and A-112.
Significantly reduces autophagic activity; when associated with A-108 and A-112.
deca-GX3 motif; required for interaction with LTO1
Putative uncharacterized protein YCR095W-A
Abolishes function; no effect on interaction with MSH4.
Phenylalanine--tRNA ligase, mitochondrial
Interaction with the F-box domain of F-box proteins
In allele sgs1-34; temperature-sensitive.
In allele sgs1-35; temperature-sensitive.
In allele sgs1-36; temperature-sensitive.
Abolishes ubiquitination and subsequent degradation.
Affects phosphorylation and the interaction with ESP1.
Putative endoplasmic reticulum mannosidase MNL2
Reduces thermal stability and enzyme activity; when associated with A-526 or C-526. No effect; when associated with D-526 or Q-526.
Reduces thermal stability and enzyme activity; when associated with D-457.
In strain: SK1, V1-09, YJM339 and YJM627.
Mitochondrial import receptor subunit TOM70
Required for nuclear membrane association and proliferation
Partially suppresses the respiratory defect of SHY1 mutants by increasing COX1 translation.
tRNA(Thr) (cytosine(32)-N(3))-methyltransferase
Required for localization at the bud neck
Strongly reduces E3 activity, but no effect on subcellular location.
General transcription and DNA repair factor IIH helicase subunit XPB
Abolished zinc-binding and folding, leading to impaired protein import into peroxisomes.
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Galactose-1-phosphate uridylyltransferase
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5
Leads to loss of SLX5 recruitment to ub-hotspots.
Ubiquitin carboxyl-terminal hydrolase MIY1
4-hydroxybenzoate polyprenyltransferase, mitochondrial
Abolished phosphorylation by ATG1, leading to increased interaction with ATG8.
Abolishes the proteolytic activity and decreases autophagy.
Decreased autophagy caused by strongly reduced interaction with ATG8.
Does not affect autophagy. Slightly decreased interaction with ATG8.
Impaired autophagy caused by decreased delipdation of ATG8. Reduced recruitment to preautophagosomal structures.
Increased recruitmentto the phagophore assembly site.
Phospho-mimetic mutant; decreased integration with ATG8, leading to impaired decreased delipdation of ATG8.
Isocitrate dehydrogenase [NADP], mitochondrial
Protein arginine methyltransferase NDUFAF7 homolog, mitochondrial
Putative ATP-dependent RNA helicase YLR419W
Loses ability to recognize arginine (R)-based ER localization signals in proteins. Recognition of C-terminal di-lysine signals present in proteins is unimpaired.
In TIF1-1; no growth at 36 degrees Celsius.
Increases about 3-fold ATP binding but reduces about 2-fold ATPase activity in vitro.
Lethal and dominant negative if overexpressed in vivo. Impairs ATP hydrolysis, RNA-helicase and translation activity in vitro.
Lethal and dominant negative if overexpressed.
Lethal in vivo and reduces ATP binding and ATPase activity in vitro.
Reduces ATP binding and ATPase activity in vitro.
Slow growth in vivo and reduces ATP binding and ATPase activity in vitro.
Probable transcriptional regulatory protein STB4
3,4-dihydroxy-2-butanone 4-phosphate synthase
Increased recombination centers protein 6
25S rRNA (cytosine(2278)-C(5))-methyltransferase
Fails to catalyze the C-5 methylation of the C2278 residue.
Lethal. Blocks the separation of the enzyme from its RNA substrate.
DNA-directed RNA polymerase I subunit RPA43
U4/U6 small nuclear ribonucleoprotein PRP4
Cytosolic Fe-S cluster assembly factor CFD1
Loss of function and disrupts heterotetramer formation.
Interaction with glutamyl-tRNA synthetase GUS1
Interaction with methionyl-tRNA synthetase MES1
Putative cyclin-dependent kinase inhibitor SPL2
Cytochrome b-c1 complex subunit 7, mitochondrial
Putative uncharacterized protein YHR214C-E
Serine/threonine-protein phosphatase 2A activator 2
Putative uncharacterized protein YHL042W
Flavin prenyltransferase PAD1, mitochondrial
The measured mass is of the truncated protein without mitochondrial targeting sequence including the mass of an N-terminal hexahistidine tag, expressed in E.coli.
Restores interactions with PRP2 mutants.
Loss of proteasomal degradation in response to shift to glucose-containing growth medium.
6-phosphogluconate dehydrogenase, decarboxylating 1
Vacuolar protein sorting-associated protein 55
Inorganic pyrophosphatase, mitochondrial
Putative glutamine amidotransferase YLR126C
Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction.
Partially active, with very weak activity toward NAD.
Transcriptional activator/repressor GIS1
Ribonucleases P/MRP protein subunit POP7
F(1)-ATPase inhibitor IF(1), mitochondrial
RNA-binding suppressor of PAS kinase protein 1
Does not strongly affect the phosphatase inhibitor function but abolishes PPCDC activity.
Phosphopantothenoylcysteine decarboxylase subunit VHS3
Interaction with PRP19 and self-interaction
No effect. Slower growth and thermosensitivity; when associated with G-33 or G-52. Complete loss of function; when associated with G-33 and G-52 or G-52 and Y-102. Complete loss of function; when associated with G-33; G-52 and G-102.
No effect. Slower growth and thermosensitivity; when associated with G-52 or G-84. Complete loss of function; when associated with G-33; G-52 and G-84.
No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-33 and G-84. Complete loss of function; when associated with G-33; G-84 and G-102.
No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-52 and G-84. Complete loss of function; when associated with G-52; G-84 and G-102.
Uncharacterized protein YML007C-A, mitochondrial
Eukaryotic translation initiation factor 2A
Abnormal endocytic recycling, but interaction with DRS2 appears normal.
Abnormal endocytic recycling, but interaction with DRS2 appears normal; when associated with A-320.
Abnormal endocytic recycling, but interaction with DRS2 appears normal; when associated with H-23.
Phospholipid-transporting ATPase accessory subunit CDC50
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
Abolished ability to promote translation elongation.
Causes impaired ribosomal translocation with an increased rate of -1 programmed ribosomal frameshift read-through during translation.
Causes resistance to fusidic acid and reduces sensitivity to sordarin.
Causes resistance to fusidic acid and sordarin.
Leads to conditional growth defects, sensitivity to translation inhibitors, and decreased translation.
N6,N6,N6-trimethyllysine; by EFM3; alternate
N6,N6-dimethyllysine; by EFM2; alternate
N6,N6-dimethyllysine; by EFM3; alternate
Prevents ADP-ribosylation of the diphthamide by diphtheria toxin.
Prevents post-translational modification of this residue to diphthamide. Results in a functional protein that is resistant to diphtheria toxin and sordarin.
Reduces sensitivity to fusidic acid and causes resistance to sordarin.
Reduces sensitivity to fusidic acid and sordarin.
Regulator of rDNA transcription protein 14
In strain: YIIc12 haplotype Ha2 and YIIc17.
Eukaryotic translation initiation factor 1A
Most catalytic properties similar to wild-type.
Reduced affinity for xylulose 5-phosphate and strongly reduced affinity for ribose 5-phosphate.
Reduces activity by over 96% and decreases affinity for thiamine pyrophosphate and xylulose 5-phosphate.
Reduces catalytic activity by about 95%.
Reduces catalytic activity by about 98%. Decreased affinity for donor substrate.
Slightly reduced affinity for xylulose 5-phosphate and strongly reduced affinity for ribose 5-phosphate.
Strongly reduced affinity for xylulose 5-phosphate and ribose 5-phosphate.
Strongly reduced catalytic activity. Decreases affinity for xylulose 5-phosphate about 15 times.
Strongly reduced catalytic activity. Strongly reduced affinity for xylulose 5-phosphate and ribose 5-phosphate.
Cytochrome c oxidase assembly protein COX20, mitochondrial
Mitochondrial distribution and morphology protein 36
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
ATP-dependent permease MDL2, mitochondrial
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19
Altered inheritance of mitochondria protein 21
Serine hydroxymethyltransferase, mitochondrial
Uncharacterized transcriptional regulatory protein YLR278C
Probable transcription repressor protein RGM1
Constitutive nuclear entry; when associated with A-522 and A-646.
Constitutive nuclear entry; when associated with A-522 and A-664.
Constitutive nuclear entry; when associated with A-646 and A-664.
N-terminal acetyltransferase B complex subunit MDM20
DNA repair and recombination protein RDH54
Abolishes exonuclease activity in vitro.
Strongly reduces exonuclease activity; when associated with D-1024; F-1043 and P-1197.
Strongly reduces exonuclease activity; when associated with P-592.
Strongly reduces exonuclease activity; when associated with R-798; D-1024 and F-1043.
Strongly reduces exonuclease activity; when associated with R-798; D-1024 and P-1197.
Strongly reduces exonuclease activity; when associated with R-798; F-1043 and P-1197.
Strongly reduces exonuclease activity; when associated with Y-710.
Heterogeneous nuclear rnp K-like protein 2
Increases translation repression of ASH1 mRNA.
Putative ISWI chromatin-remodeling complex subunit YPL216W
Abolishes the ability to suppress glucose-induced cAMP signaling when overexpressed.
Decreases the ability to suppress glucose-induced cAMP signaling when overexpressed.
No interaction with CTK2. Still interacts with CTK1.
Mitochondrial import inner membrane translocase subunit TIM50
Sufficient for dosage suppression of COX2 mutation
Sufficient for general mitochondrial translation
Disrupts iron-sulfur (Fe-S) cluster assembly.
Fails to complement an isu1 deletion mutation.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-72.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-94.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-72 and S-94.
Iron sulfur cluster assembly protein 1, mitochondrial
No growth; no interaction with frataxin and SSQ1.
Wild-type growth; no interaction with SSQ1.
Putative uncharacterized protein YPR203W
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
Potassium-activated aldehyde dehydrogenase, mitochondrial
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-114, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-114 and S-178.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-114 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-99, S-114, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-77, S-99, S-114, S-178 and S-192.
Mitochondrial intermembrane space cysteine motif-containing protein MIX23
Disrupts the interaction with YTM1 and cannot sustain growth.
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-470.
Weakens the interaction with YTM1, impairs pre-rRNA processing and leads to a deficiency in 60S ribosomal subunits. Disrupts the interaction with YTM1 and cannot sustain growth; when associated with R-790.
DNA repair and recombination protein RAD52
Very long-chain fatty acid transport protein
Glycolytic genes transcriptional activator GCR2
Abolishes RSP5 binding site and consequently ubiquitination.
Abolishes formation of ubiquitinated protein forms.
Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin, aggregation, and prion-inducing ability.
Reduces, but does not abolish the ability to promote [PSI+] induction.
3-methyl-2-oxobutanoate hydroxymethyltransferase
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
In upc2-1; increases aerobic sterol synthesis and uptake. Increases also aerobic expression of DAN1 and sensibility to NaCl and LiCl.
Anaphase-promoting complex subunit CDC23
In CDC23-1; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-2; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-37; G2/M cell cycle arrest at 30 degrees Celsius.
In CDC23-39; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-40; G2/M cell cycle arrest at 37 degrees Celsius; when associated with R-103.
In CDC23-40; G2/M cell cycle arrest at 37 degrees Celsius; when associated with V-573.
In CDC23-41; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-44; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-47; G2/M cell cycle arrest at 37 degrees Celsius; when associated with G-213.
In CDC23-47; G2/M cell cycle arrest at 37 degrees Celsius; when associated with W-583.
In CDC23-49; G2/M cell cycle arrest at 37 degrees Celsius; when associated with E-306.
In CDC23-49; G2/M cell cycle arrest at 37 degrees Celsius; when associated with P-326.
In CDC23-4; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-50; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-51; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-52; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-53; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-54; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-56; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-6; G2/M cell cycle arrest at 36 degrees Celsius.
In G5037; blocks formation of mature cytochrome b mRNA.
In M2011; leads to absence of functional cytochrome b, but does not affect splicing.
Putative uncharacterized protein YLR156W
Putative uncharacterized protein YLR159W
Putative uncharacterized protein YLR161W
Endoplasmic reticulum membrane protein complex subunit 10
Uncharacterized membrane glycoprotein YNR065C
General negative regulator of transcription subunit 4
Changed the substrate specificity of the transporter from zinc to iron.
Phosphatidylserine decarboxylase 2 alpha chain
Phosphatidylserine decarboxylase 2 beta chain
Phosphatidylserine decarboxylase proenzyme 2
tRNA (34-2'-O)-methyltransferase regulator RTT10
No pyridoxal 5'-phosphate synthesis activity. Retains ability to isomerize dihydroxyacetone phosphate to glyceraldehyde 3-phosphate.
Pyridoxal 5'-phosphate synthase subunit SNZ1
Schiff-base intermediate with D-ribose 5-phosphate
Thiamine biosynthesis regulatory protein
Putative pterin-4-alpha-carbinolamine dehydratase
Geranylgeranyl transferase type-2 subunit alpha
Greatly reduces actin-binding and actin-bundling activity.
Moderately reduces actin-binding and actin-bundling activity.
DNA-directed RNA polymerases I and III subunit RPAC1
CDC48-associated ubiquitin-like/zinc finger protein 1
Topoisomerase I damage affected protein 11
Vacuolar protein sorting/targeting protein VPS10
Almost complete loss of SAM binding. No effect on growth and ribosome biogenesis.
Loses its exclusive nucleolar localization and mislocalizes to the cytoplasm.
Loss of substrate rRNA binding. No effect on growth.
Ribosomal RNA small subunit methyltransferase NEP1
Defect in promoter association and change in chromatin structure.
Formamide-, hydroxyurea and UV-hypersensitivity, destabilization of ARP4, suppressor of TY phenotype and elevated levels of MSN2/MSN4-regulated genes. Lethal; when associated with A-23.
Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with S-23.
Lethal; when associated with D-161. Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with A-159.
No histone acetyltransferase activity at 37 degrees Celsius.
No histone acetyltransferase activity at 37 degrees Celsius. Defect in promoter association and change in chromatin structure.
Translation machinery-associated protein 7
Abolishes DNA polymerase activity and increases UV-induced mutations.
Abolishes POL30-binding; when associated with A-627.
Abolishes POL30-binding; when associated with A-628.
Decreases efficiency of nucleotide incorporation.
General negative regulator of transcription subunit 2
Auxiliary; required for high transcriptional activity under nonrepressive growth conditions
Inhibitory region; AdoMet responsiveness; required for interaction with MET30
Required for interaction with MET31 and MET32
Transcriptional activator of sulfur metabolism MET4
Structural maintenance of chromosomes protein 5
Abolishes the physical interaction with ARG82 and impairs function; when associated with G-94 and D-95.
Arginine metabolism regulation protein I
Decreases the physical interaction with ARG82.
Decreases the physical interaction with ARG82. Abolishes the physical interaction with ARG82 and impairs function; when associated with D-95 and R-103.
Decreases the physical interaction with ARG82. Abolishes the physical interaction with ARG82 and impairs function; when associated with G-94 and R-103.
Putative uncharacterized protein YHR050W-A
Cold-sensitive; reduces catalytic activity more than 20-fold at 14 degrees Celsius.
Reduces catalytic activity between 5- to 25-fold.
Abolished translocase activity, characterized by impaired ability to extract tail-anchored proteins from membranes.
Abolished translocase activity, characterized by impaired ability to unfold and extract tail-anchored proteins from membranes.
Does not impair ability to extract tail-anchored proteins from membranes. Does not affect formation of a homohexamer.
Impaired ability to extract tail-anchored proteins from membranes.
Impaired ability to extract tail-anchored proteins from membranes. Does not affect formation of a homohexamer.
Impaired ability to extract tail-anchored proteins from membranes. Impaired formation of a homohexamer.
Impaired formation of a homohexamer. Impaired ability to extract tail-anchored proteins from membranes.
Loss of ATPase activity. Does not affect formation of a homohexamer.
Outer mitochondrial transmembrane helix translocase
Causes a dramatic decrease in the affinity of SGF11 for SUS1.
Moderately impairs deubiquitination activity of the SAGADUB module.
Moerately decreases the affinity of SGF11 for SUS1.
Reduces deubiquitination activity of the SAGADUB module; when associated with A-57.
Reduces deubiquitination activity of the SAGADUB module; when associated with A-60.
Strongly reduces deubiquitination activity of the SAGADUB module.
Vacuolar protein sorting-associated protein 9
Interaction with the N-terminal Pro (Pro/N-degron) of proteins that are targeted for degradation
Vacuolar import and degradation protein 24
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with S-315 and S-393.
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with Y-105 and S-315.
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with Y-105 and S-393.
Increases 3-fold the ATPase activity and a higher RNA/DNA heteroduplexes unwinding activity in vitro. Leads to 13% of wild-type ATPase activity and lower RNA/DNA heteroduplexes unwinding activity in vitro; when associated with A-92.
Lethal in vivo and impairs ATPase with 2-5% of wild-type ATPase activity, a 20-fold higher KM for ATP and prevents RNA/DNA heteroduplexes unwinding activity in vitro. Leads to 13% of wild-type ATPase activity and higher RNA/DNA heteroduplexes unwinding activity in vitro; when associated with A-389.
Lethal in vivo and leads to 70-80% of wild-type ATPase activity, a 2-fold lower KM for ATP and a reduced RNA/DNA heteroduplexes unwinding activity in vitro.
Lethal in vivo and leads to less than 30% of wild-type ATPase activity and a 2-fold lower KM for ATP in vitro.
Slow growth at 18 and 16 degrees Celsius, no growth at 14 degrees Celsius and drastic decrease of the amount of 40S ribosomal subunits at 30 degrees Celsius in vivo. Leads to 70-80% of wild-type ATPase activity, a 2-fold lower KM for ATP and a slightly reduced RNA/DNA heteroduplexes unwinding activity in vitro.
Reduced growth at 25 degrees and lethal at 37 degrees.
NADPH-dependent diflavin oxidoreductase 1
DSC E3 ubiquitin ligase complex subunit 3
Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
Reduces the transferase reaction 12-fold.
Reduces the transferase reaction 5-fold.
Topoisomerase I damage affected protein 4
Medium-chain fatty acid ethyl ester synthase/esterase 1
Ferric/cupric reductase transmembrane component 2
NADPH-dependent alpha-keto amide reductase
15 X 6 AA tandem repeats of S-[TA]-W-G-G-[AQ]
Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP.
No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152.
No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150.
No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152.
Severely temperature-sensitive. Defective in pre-mRNA splicing.
U1 small nuclear ribonucleoprotein 70 kDa homolog
Reduces catalytic activity towards homoaconitate by 45% and increases the activity towards aconitate by a factor 116.
Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule.
tRNA-splicing endonuclease subunit SEN34
Heme-responsive zinc finger transcription factor HAP1
Heme-responsive; required for HMC formation
Transcription and mRNA export factor SUS1
Sulfite reductase [NADPH] flavoprotein component
NPC intracellular sterol transporter 1-related protein 1
Sphingolipids mislocalization and no growth at 38 degrees Celsius.
Serine/threonine-protein phosphatase 2B catalytic subunit A2
Serine/threonine-protein kinase Haspin homolog ALK2
Mitochondrial protein import protein MAS5
Necessary for HAP1 repression in the absence of heme
Putative ATP-dependent RNA helicase ECM32
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase
Ubiquitin ligase complex F-box protein UFO1
Competitive binding site of NUP57 and NUP82
Required for association with NUP57 subcomplex
Putative uncharacterized protein YBR201C-A
AbA resistant; when associated with F-137.
AbA resistant; when associated with Y-157.
Inositol phosphorylceramide synthase catalytic subunit AUR1
ATP-dependent 6-phosphofructokinase subunit beta
Abolishes sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'D-724' in subunit alpha.
Drastically reduces sensitivity of the holoenzyme to ATP inhibition.
Increases the KM for fructose 6-phosphate 50 fold.
Reduces maximal activity of the holoenzyme by 30%.
Reduces maximal activity of the holoenzyme by 50%. Completely abolishes catalytic activity; when associated with 'T-309' or 'S-356' in subunit alpha.
Reduces maximal activity of the holoenzyme by less than 25%.
Reduces sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'S-859' in subunit alpha.
5 X 5 AA repeats of [DE]-[DE]-[DE]-K-K; highly charged region
Abolishes interaction with SBA1. Reduces client protein activation activity, leading to growth defect at high temperatures.
Causes a 90% reduction in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Causes a 98% reduction in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Decreases AHA1 binding affinity and substantially reduces client protein activation activity.
Decreases AHA1 binding affinity, but has no effect on client protein activation activity.
Enhances ATPase activity and client protein activation.
Enhances homodimerization, ATPase activity and client protein activation.
Induces a 6-fold increase in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Induces a 6-fold increase in ATPase activity.
Induces a loss of ATPase activity. Can be reactivated by AHA1.
Induces a total loss of function at 34 degrees Celsius. Abolishes interaction with SBA1.
No effect on ATPase activity. Reduces client protein activation activity, leading to growth defect at high temperatures.
Reduces ATPase activity and client protein activation.
Reduces client protein activation activity, leading to growth defect at high temperatures.
Reduces client protein activation activity. Resistant to ATPase activation by AHA1.
Reduces client protein activation activity; when associated with K-579.
Reduces client protein activation activity; when associated with T-576.
Reduces homodimerization, ATPase activity and client protein activation.
Specifically reduces the activation of the exogenous ligand glucocorticoid receptor.
Abolishes GTP-binding; no strong effect in vivo.
Loss of function; abolishes association with PI(4)P and PI(5)P.
Loss of function; strongly decreases association with PI(4)P and PI(5)P.
Temperature-sensitive; no effect at 23 degrees Celsius but loss of function at 37 degrees Celsius.
Temperature-sensitive; no effect at 23 degrees Celsius but loss of function at 37 degrees Celsius; abolishes interaction with itself.
In ZAP1-1up; interferes with the zinc-dependent repression of the ZRT1 promoter but does not prevent full induction of the ZRT1 gene.
Results in a significant defect in zinc responsiveness; when associated with A-202 and A-203.
Results in a significant defect in zinc responsiveness; when associated with A-202 and A-207.
Results in a significant defect in zinc responsiveness; when associated with A-203 and A-207.
Zinc-responsive transcriptional regulator ZAP1
Dominant active allele that abolishes intrinsic GTPase activity.
Dominant negative allele unable to undergo the GTP-induced conformational change.
Guanine nucleotide-binding protein alpha-2 subunit
Locks GPA2 in its activated GTP-bound form and abrogates the negative control by RGS2.
In DCP1-17; partial loss of mRNA-decapping activity.
In DCP1-19; partial loss of mRNA decapping activity. In DCP1-43; strong loss of mRNA-decapping activity; when associated with A-48 and A-50.
In DCP1-1; strong loss of mRNA decapping activity.
In DCP1-25; partial loss of mRNA-decapping activity. In DCP1-44; strong loss of mRNA-decapping activity; when associated with A-48 and A-50.
In DCP1-2; strong loss of mRNA decapping activity at 36 degrees Celsius.
In DCP1-31; partial loss of mRNA decapping activity.
In DCP1-32; partial loss of mRNA decapping activity.
In DCP1-33; partial loss of mRNA decapping activity.
In DCP1-33; strong loss of mRNA decapping activity.
In DCP1-35; partial loss of mRNA decapping activity.
In DCP1-4; partial loss of mRNA decapping activity. In DCP1-43; strong loss of mRNA-decapping activity; when associated with A-187 and A-188. In DCP1-44; strong loss of mRNA-decapping activity; when associated with A-216 and A-219.
Partial loss of mRNA-decapping activity.
Strong loss of mRNA-decapping activity; when associated with A-217 and A-221.
Strong loss of mRNA-decapping activity; when associated with A-37; A-38 and A-217.
Strong loss of mRNA-decapping activity; when associated with A-37; A-38 and A-221.
Loss of interaction with ATG18 and VAC7.
Loss of interaction with ATG18, FAB1 and VAC7. No loss of interaction with FIG4.
Vacuole morphology and inheritance protein 14
Bifunctional purine biosynthesis protein ADE17
Proton acceptor; for AICAR formyltransferase activity
Proton donor/acceptor; for FAICAR cyclization activity
Putative uncharacterized protein YER138W-A
Meiotic sister-chromatid recombination protein 3
Truncated non-functional cytochrome oxidase 1
Cytochrome c mitochondrial import factor CYC2
Lysine biosynthesis regulatory protein LYS14
Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-541.
Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-669.
In ufd3-2; severe reduction in the degradation of short-lived ubiquitin-fusion proteins. No defect in the interaction with CDC48.
Loss of interaction with CDC48 without affecting binding to ubiquitin.
Loss of interaction with HSE1 and ubiquitin without affecting general ubquitination levels. Prevents protease CSP1 sorting into the vacuole.
Loss of interaction with HSE1 without affecting binding to ubiquitin or general ubquitination levels. Prevents protease CSP1 sorting into the vacuole.
No binding to ubiquitin and no interaction with CDC48.
No binding to ubiquitin but does not affect the interaction with CDC48.
No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-222.
No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-265.
No binding to ubiquitin. Reduces cell growth at high temperatures (37 degrees Celsius). Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with A-222 and A-265.
Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-417.
Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-434.
Abolishes inositol polyphosphate binding; when associated with A-127 and A-130.
Abolishes inositol polyphosphate binding; when associated with A-127 and A-134.
Abolishes inositol polyphosphate binding; when associated with A-130 and A-134.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with A-26 and A-131.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with F-22 and A-131.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with F-22 and A-26.
Vacuolar transporter chaperone complex subunit 2
1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
Imidazole glycerol phosphate synthase hisHF
No activity. Uncoupling of glutaminase activity from the cyclase activity.
ISWI chromatin-remodeling complex ATPase ISW2
Putative oligopeptide transporter YGL114W
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Increased recombination centers protein 21
In FAR1-22P; induces cell cycle arrest in the absence of alpha-factor.
Prevents cell cycle-dependent degradation of FAR1.
Cytochrome c oxidase assembly protein COX18, mitochondrial
DNA-directed RNA polymerase I subunit RPA190
Ran-specific GTPase-activating protein 30
Chromosome transmission fidelity protein 8
Cytochrome B pre-mRNA-processing protein 1
Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
Increased localization to the cytoplasm.
Slightly increased localization to the cytoplasm.
Slightly increased localization to the nucleus.
Vacuolar import and degradation protein 28
Vesicle-associated membrane protein-associated protein SCS22
Does not affect self-association in the presence of NVJ1 but fails to undergo self-association in the presence of ATG13. Fails to support the cvt pathway, but does not affect the PMN pathway; when associated with R-60.
Fails to support the Cvt pathway, but does not affect the PMN pathway; when associated with R-62.
Fails to undergo self-association in the presence of NVJ1 or ATG13.
In allele AFT1-1UP; which is constitutively activated.
Iron-regulated transcriptional activator AFT1
Required for membrane-association, autophagic function during starvation and normal autophagosome morphology
Vacuolar protein sorting-associated protein 30
Cold-sensitive. Abolishes interaction with BRF1/TDS4.
Cold-sensitive. No effect on interaction with BRF1/TDS4.
DNA-directed RNA polymerase III subunit RPC6
Temperature-sensitive; cold-sensitive. Abolishes interaction with BRF1/TDS4. Stabilizes Pol III open complex formation.
tRNA (uracil(54)-C(5))-methyltransferase
Centromere DNA-binding protein complex CBF3 subunit B
Serine/arginine (SR)-type shuttling mRNA binding protein GBP2
Shifts localization of the protein to the cytoplasm at steady state.
Vacuolar ATPase assembly integral membrane protein VPH2
Eukaryotic initiation factor 4F subunit p150
In TIF4631-213; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation.
Mannosyl phosphorylinositol ceramide synthase CSH1
In MLC1-11; abolishes interaction with MYO2 and IQG1 and reduces interaction with MYO1. Leads to mislocalization of IQG1 and a severe defect in cytokinesis.
In MLC1-1; causes a defect in septum formation.
In MLC1-5; causes a defect in septum formation.
In MLC1-93; reduces interaction with MYO1, but does not cause any defect in cytokinesis.
Maintenance of telomere capping protein 6
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]
Cardiolipin-specific deacylase 1, mitochondrial
Loss of function; when associated with S-296.
Loss of function; when associated with S-298.
Loss of function; when associated with S-307.
Loss of function; when associated with S-317.
Loss of function; when associated with S-330.
Loss of function; when associated with S-340.
Mitochondrial intermembrane space import and assembly protein 40
Membrane-anchored lipid-binding protein SIP3
Glutathione peroxidase-like peroxiredoxin 1
Transmembrane E3 ubiquitin-protein ligase 1
DNA damage checkpoint control protein MEC3
Induces collapsed, globular or grape-like mitochondria.
Induces collapsed, globular or grape-like mitochondria; when associated with A-225.
Induces collapsed, globular or grape-like mitochondria; when associated with A-354.
DNA-directed RNA polymerase III subunit RPC9
In 1014; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; partially K1 killer toxin-sensitive; when associated with DEL-GSC2.
In 1082; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with DEL-GSC2.
In 1093; temperature-sensitive mutant; higher beta-glucan content of cells; when associated with I-605 and DEL-GSC2.
In 1093; temperature-sensitive mutant; higher beta-glucan content of cells; when associated with T-761 and DEL-GSC2.
In 1104; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with E-920 and DEL-GSC2.
In 1104; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with V-823 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with G-932; D-934; Y-1020; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; D-934; Y-1020; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; D-934; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; D-934; Y-1020 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; Y-1020; N-1047 and DEL-GSC2.
In 1125; temperature-sensitive mutant; lower beta-glucan content of cells and partial K1 killer toxin-resistant phenotype; when associated with Y-1258 and DEL-GSC2.
In 1125; temperature-sensitive mutant; lower beta-glucan content of cells and partially K1 killer toxin-resistant; when associated with D-1520 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with N-329; N-335 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-146; N-329 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-146; N-335 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with F-872; K-907 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with F-872; S-982 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with K-907; S-982 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with N-877; P-977 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with N-877; S-899 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with S-899; P-977 and DEL-GSC2.
In 1163; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with L-713 and DEL-GSC2.
In 1163; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-722 and DEL-GSC2.
In A6; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with DEL-GSC2.
In ACR1A3; selectively resistant to antibiotic arborcandin C.
In ACR79-5; selectively resistant to antibiotic arborcandin C.
In F4; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with DEL-GSC2.
Causes hypersensitivity to DNA-damage-inducing agents.
Transcription factor TFIIIB component B""" /id="PRO_0000072498
In HRT1-C81Y; defects in ubiquitin ligase activity.
In RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-72.
In RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-81.
CCA tRNA nucleotidyltransferase, mitochondrial
No effect. Compensates K-189 mutant; when associated with K-189.
Temperature-sensitive mutation; reduced ability to incorporate AMP and CMP at 37 degrees Celsius. Defects are compensated by mutant N-64; when associated with N-64.
4 X 36 AA approximate repeats, Ser/Thr-rich
6 X 27 AA approximate repeats, Ser/Thr-rich
Catabolic L-serine/threonine dehydratase
Decrease of ER lumenal misfolded protein degradation.
Decrease of ER lumenal misfolded protein degradation. No effect on interaction with CDC48, HRD3, KAR2, UBX2, HRD1 or EMP47.
UDP-N-acetylglucosamine transporter YEA4
11 X 12 AA approximate tandem repeats, Ser-rich
Uncharacterized membrane protein YNL143C
ATP synthase subunit epsilon, mitochondrial
Probable vacuolar amino acid transporter YPQ1
Abolishes palmitoyltransferase activity and interaction with SHR5.
Abolishes palmitoyltransferase activity.
Leads to defects in A1 and A2 processing.
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8
General transcription and DNA repair factor IIH subunit SSL1
tRNA A64-2'-O-ribosylphosphate transferase
Uncharacterized membrane protein YIL089W
Intracellular protein transport protein USO1
In ASI13-1; dominant active and constitutively nuclear localized transcription factor.
In STP1-102; prevents proteolytic processing. Activates transcription also in its unprocessed form; when associated with 27-AASGAA-32.
In STP1-133; impairs cytoplasmic retention, resulting in a dominant active transcription factor. Activates transcription also in its unprocessed form; when associated with A-66.
Probable glutathione-independent glyoxalase SNO4
Altered inheritance rate of mitochondria protein 25
Uncharacterized membrane protein YLR050C
In sgo1-100; induces a lack of response when chromosomes that are not under tension.
In sgo1-700; induces a lack of response when chromosomes that are not under tension.
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate
Peroxisomal long-chain fatty acid import protein 1
Abolishes membrane association. Lethal; when associated with S-205.
Abolishes membrane association. Lethal; when associated with S-206.
Decreases GTP binding and GTP hydrolysis.
Decreases GTP binding and increases GTP hydrolysis.
Locks YPT1 in the GTP-bound form by reducing GTP hydrolysis rate 40-fold.
Cytochrome c oxidase subunit 13, mitochondrial
ATP synthase subunit delta, mitochondrial
Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and L-1773 or D-1769; L-1773; S-1879 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and S-1771 or D-1769; S-1771; S-1879 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and S-1879.
Does not affect oligomerization; when associated with S-1771 and L-1773 or S-1771; L-1773; S-1879 and E-1881.
Loss of transferase activity; when associated with S-1772.
Loss of transferase activity; when associated with S-1774.
External NADH-ubiquinone oxidoreductase 1, mitochondrial
Ubiquitin domain-containing protein DSK2
Arf guanine nucleotide exchange factor SYT1
Decreases interaction with GCN1, but not with ACT1, and prevents inhibition of GCN2 activity in amino acid-starved cells; when associated with A-102.
Decreases interaction with GCN1, but not with ACT1, and prevents inhibition of GCN2 activity in amino acid-starved cells; when associated with A-106.
Slightly increases interaction with GCN1 and ACT1 and does not prevent inhibition of GCN2 activity in amino acid-starved cells; when associated with A-87.
Slightly increases interaction with GCN1 and ACT1 and does not prevent inhibition of GCN2 activity in amino acid-starved cells; when associated with A-90.
In PTF1-2; decreases the catalytic efficiency 20-fold.
In PTF1-5; decreases the catalytic efficiency 12-fold.
Peptidyl-prolyl cis-trans isomerase ESS1
6-phosphogluconolactonase-like protein 1
Conserved oligomeric Golgi complex subunit 2
Endosomal/prevacuolar sodium/hydrogen exchanger
Impairs partially resistance to osmotic stress and hygromycin.
Impairs protein-trafficking to the vacuole.
Impairs resistance to osmotic stress and hygromycin, and blocks protein-trafficking to the vacuole.
Impairs resistance to osmotic stress and hygromycin.
Asparagine synthetase [glutamine-hydrolyzing] 1
Important for beta-aspartyl-AMP intermediate formation
Decreased phosphorylation by mTOR/TOR1, leading to reduced ribosome reactivation in response to nutrient replenishment.
Mimics phosphorylation by mTOR/TOR1, leading to decreased ability to mediate ribosome hibernation.
Probable alpha-1,6-mannosyltransferase MNN11
Abolished disulfide bond, leading to impaired conformational state; when associated with A-272.
Abolished disulfide bond, leading to impaired conformational state; when associated with A-332.
Significantly decreased protease activity.
Spindle pole body-associated protein CIK1
26S proteasome regulatory subunit 7 homolog
Inner membrane mitoribosome receptor MBA1, mitochondrial
Signal recognition particle receptor subunit beta
Mediator of RNA polymerase II transcription subunit 8
UDP-N-acetylglucosamine transferase subunit ALG13
Mitochondrial distribution and morphology protein 10
Transcription initiation factor TFIID subunit 9
Impaired tail-anchored protein insertion and impaired GET3 localization and loss of the GET1-GET2 heterotetramer; when associated with A-150.
Impaired tail-anchored protein insertion, impaired GET3 localization and loss of the GET1-GET2 heterotetramer; when associated with A-157.
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Anaphase-promoting complex subunit CDC26
In VMA4-1; is rapidly degraded at 37 degrees Celsius.
Increases ATPase activity 2.1-fold by increasing Vmax for ATP hydrolysis 2-fold. Reduces ATPase activity by 90% and produces structurally aberrant V-ATPase complexes; when associated with A-233.
Reduces ATPase activity by 74%. Reduces ATPase activity by 90% and produces structurally aberrant V-ATPase complexes; when associated with A-202.
Reduces ATPase activity by 88%. Prevents assembly of V1 subunits at the membrane.
No effect in conferring Congo red resistance; when associated with A-130.
No effect in conferring Congo red resistance; when associated with A-208.
Reduced interaction with WW domain of RSP5. No interaction with WW domain of RSP5; when in association with 82-QA-83.
Reduced interaction with WW domain of RSP5. No interaction with WW domain of RSP5; when in association with A-105.
Mitochondrial biogenesis regulation protein 1
Leads to partial block in enzymatic activity.
Vacuolar serine-type carboxypeptidase ATG42
Impairs correct nuclear localization; when associated with T-19 and T-20.
Impairs correct nuclear localization; when associated with T-19 and T-23.
Impairs correct nuclear localization; when associated with T-20 and T-23.
Leads to temperature-dependent mislocalization of nucleoporins (nups) and the pore-membrane protein POM152.
Eukaryotic peptide chain release factor GTP-binding subunit
In FUR1-5; causes resistance to 5-fluorouracil (5FU).
In FUR1-7; causes resistance to 5-fluorouracil (5FU).
In FUR1-8; causes resistance to 5-fluorouracil (5FU).
In FUR1-9; causes resistance to 5-fluorouracil (5FU).
Ankyrin repeat-containing protein YGL242C
Interferes with correct plasma membrane localization.
Loss of function; when associated with Q-323.
Loss of function; when associated with Q-324.
Peptidyl-prolyl cis-trans isomerase CYP8
ABC transporter ATP-binding protein ARB1
Abolished ATPase activity, leading to impaired ability to stimulate VMS1 activity.
Abolished ATPase activity, leading to impaired ability to stimulate VMS1 activity; when associated with 229-D-E-230.
Abolished ATPase activity, leading to impaired ability to stimulate VMS1 activity; when associated with D-519.
Ribosome-associated molecular chaperone SSB2
10-fold decrease in interaction with ISU1.
Iron-sulfur cluster biogenesis chaperone, mitochondrial
Mitochondrial import receptor subunit TOM6
Cruciform cutting endonuclease 1, mitochondrial
Induces change in substrate recognition on ATP binding
Required for oligomerization; adenylyl-sulfate kinase-like
Abolishes binding to phosphatidylinositol 4-phosphate.
Abolishes both cholesterol binding and biological function.
Reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
Slight reduction in cholesterol transport.
Slight reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
Strong reduction in cholesterol binding without affecting phosphatidylinositol 4-phosphate binding.
Strong reduction in cholesterol transport.
Strong reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
D-lactate dehydrogenase [cytochrome] 1, mitochondrial
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-177.
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-397.
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-177 and A-397.
Predominantly cytoplasmic; when associated with A-159, A-177 and S-397.
Significant reduction in phosphorylation; when associated with A-397. Complete inhibition of phosphorylation; when associated with A-159 and A-397. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-397. Predominantly cytoplasmic; when associated with S-99, A-159 and A-397.
Significant reduction in phosphorylation; when associated with A-397. Complete inhibition of phosphorylation; when associated with A-177 and A-397. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-177 and A-397. Predominantly cytoplasmic; when associated with S-99, A-177 and A-397.
Significant reduction in phosphorylation; when associated with either A-159 or A-177. Complete inhibition of phosphorylation; when associated with A-159 and A-177. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-177. Predominantly cytoplasmic; when associated with S-99; A-159 and A-177.
Mannan polymerase II complex ANP1 subunit
DNA-directed RNA polymerases I and III subunit RPAC2
Truncated transposon Ty1-A Gag-Pol polyprotein
Slightly increases affinity for poly-URNA ends.
U6 snRNA-associated Sm-like protein LSm5
Signal recognition particle subunit SEC65
Translation machinery-associated protein 20
Y' element ATP-dependent helicase YFL066C
Checkpoint serine/threonine-protein kinase BUB1
Ribonucleases P/MRP protein subunit POP3
The measured mass includes the mass of an N-terminal hexahistidine tag, expressed in E.coli.
Coiled-coil domain-containing protein YLR146W-A
2-deoxyglucose-6-phosphate phosphatase 2
Peroxisomal adenine nucleotide transporter 1
Structural maintenance of chromosomes protein 2
Serine hydroxymethyltransferase, cytosolic
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI12
In gwt1-10; impairs the transport of detergent-resistant microdomain-associated membrane proteins TAT2 and FUR4.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-362 and A-479.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and A-479.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and P-362.
In gwt1-20; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins.
In gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with D-259.
In gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-209.
Resistant to the drug 1-[4-butylbenzyl]isoquinoline (BIQ).
Mitochondrial tRNA-specific 2-thiouridylase 1
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Abolishes phosphorylation; when associated with A-12; A-16; A-157; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-227.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-42; A-157; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-16; A-42; A-157; A-169; A-173; A-176; A-227 and A-239.
Mitochondrial phosphate carrier protein, N-terminally processed
N-acetylserine; in Mitochondrial phosphate carrier protein, N-terminally processed
In IPL1-1; causes missegregation of chromosomes at 37 degrees Celsius.
In IPL1-2; causes missegregation of chromosomes at 37 degrees Celsius.
In IPL1-4; causes missegregation of chromosomes at 37 degrees Celsius.
Cytoplasmic dynein intermediate light chain DYN3
Cytochrome c oxidase assembly factor 3, mitochondrial
Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-311.
Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-312.
Abolishes copper resistance but not cadmium resistance; when associated with A-298.
Abolishes copper resistance but not cadmium resistance; when associated with A-300.
Confers localization to the plasma membrane and cadmium transport function.
Increased stability following gamma-irradiation and loss of phosphorylation by DUN1. Causes lethality in rnr1-W688G strain which has a mutation in RNR1.
Ribonucleotide reductase inhibitor protein SML1
In SUB2-100; no growth at 37 degrees Celsius.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; G-83 and M-142.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; G-83 and T-146.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; M-142 and T-146.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-83; M-142 and T-146.
In SUB2-201; no growth at 37 degrees Celsius; when associated with G-173 and F-403.
In SUB2-201; no growth at 37 degrees Celsius; when associated with R-122 and F-403.
In SUB2-201; no growth at 37 degrees Celsius; when associated with R-122 and G-173.
In SUB2-5; no growth at 16 degrees Celsius.
No growth at 37 degrees Celsius; when associated with DEL-135.
No growth at 37 degrees Celsius; when associated with G-8.
Does not affect autophagy; when associated with A-1086.
Does not affect autophagy; when associated with A-249.
Leads to a severely reduced activity of autophagy and a dispersed localization in the cytoplasm.
Phospho-mimetic mutant; does not affect autophagy; when associated with D-1086.
Phospho-mimetic mutant; does not affect autophagy; when associated with D-249.
RNA polymerase I-specific transcription initiation factor RRN10
Sensitive to high expression protein 9, mitochondrial
Vacuolar protein sorting-associated protein 41
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited
Uncharacterized vacuolar membrane protein YML018C
Abolishes localization to the cis-Golgi network membrane.
Protein increasingly targeted to the cis-Golgi and endoplasmic reticulum membranes. No effect on ceramide binding.
Strongly inhibits ceramide binding, and interferes with phospholipid binding. Abolishes localization to the cis-Golgi network membrane.
Mitochondrial thiamine pyrophosphate carrier 1
Probable glutamine amidotransferase DUG3
Restriction of telomere capping protein 3
Loss of function; when associated with S-112.
Loss of function; when associated with S-116.
RNA polymerase II subunit B1 CTD phosphatase RTR1
Chromatin modification-related protein EAF3
3-oxoacyl-[acyl-carrier-protein] synthase homolog
Mitochondrial distribution and morphology protein 34
Mitochondrial oxaloacetate transport protein
Required for respiratory growth protein 7, mitochondrial
Benzil reductase ((S)-benzoin forming) IRC24
UTP--glucose-1-phosphate uridylyltransferase
Sodium/potassium exporting P-type ATPase 1
U4/U6 snRNA-associated-splicing factor PRP24
18S rRNA (guanine(1575)-N(7))-methyltransferase
Can only partially restore the slow-growth phenotype and the random budding pattern of a null mutant.
Fails to ctalyze the N-7 methylation of the G1575 residue.
No effect on growth and budding pattern.
Abolishes splitting of stalled ribosomes. Defective activation of the ribosome quality control (RQC) pathway. Does not affect the association of the RQT complex with ribosomes.
U6 snRNA-associated Sm-like protein LSm7
Altered inheritance of mitochondria protein 41, mitochondrial
Moderately reduces hydrolysis, and causes a 10-fold reduction in transglycosylation activity.
Cytochrome c oxidase subunit 26, mitochondrial
Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly.
Mitochondrial respiratory chain complexes assembly protein YTA12
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Abolishes autophosphorylation; when associated with A-257 and A-357.
Abolishes autophosphorylation; when associated with A-47 and A-257.
Abolishes autophosphorylation; when associated with A-47 and A-357.
Pentamidine resistance factor, mitochondrial
Mitochondrial distribution and morphology protein 38
Altered inheritance of mitochondria protein 34, mitochondrial
22 X 15 AA approximate repeats, Ser-rich
Disrupts homotypic domain interactions; when associated with D-111 and D-113.
Disrupts homotypic domain interactions; when associated with D-111 and D-118.
Disrupts homotypic domain interactions; when associated with D-113 and D-118.
Disrupts homotypic domain interactions; when associated with D-133 and D-144.
Disrupts homotypic domain interactions; when associated with D-133 and D-196.
Disrupts homotypic domain interactions; when associated with D-144 and D-196.
Increased shedding, associated with loss of GPI-anchor site.
Maintenance of telomere capping protein 1
In SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius.
In saf1-1; impairs the protein stability.
In saf1-2; impairs the protein stability.
In saf1-3; impairs the protein stability.
In saf1-4; impairs the protein stability; when associated with K-263.
In saf1-4; impairs the protein stability; when associated with K-264.
In saf1-5; impairs the interaction with SKP1.
In saf1-6; impairs the interaction with SKP1.
U3 small nucleolar RNA-associated protein 8
Prevents correct localization of ASH1 mRNA.
Prevents correct localization of ASH1 mRNA; when associated with E-343.
Prevents correct localization of ASH1 mRNA; when associated with E-361.
Cytochrome c oxidase subunit 9, mitochondrial
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82
In LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression.
In LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression.
Target of rapamycin complex subunit LST8
Probable dolichyl-phosphate-mannose--protein mannosyltransferase 7
No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F and Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization.
No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F inhibition of polymerization. Increases penetrance of Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization.
Altered inheritance of mitochondria protein 46, mitochondrial
Lethal and prevents release of U14 snoRNA from pre-ribosomes.
Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-225.
Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-227.
Leads to 30 percent decreased oxidoreductase activity.
Leads to decreased oxidoreductase activity.
Y' element ATP-dependent helicase YPR204W
Cytochrome c oxidase assembly protein COX14
Putative metabolite transport protein YDL199C
Putative aryl-alcohol dehydrogenase AAD16
Uncharacterized trans-sulfuration enzyme YHR112C
Maintenance of telomere capping protein 3, mitochondrial
Histone-lysine N-methyltransferase, H3 lysine-4 specific
Uncharacterized mitochondrial outer membrane protein YDR381C-A
DNA damage-responsive transcriptional repressor RPH1
Uncharacterized helicase-like protein YBL111C
Functional kinase, that can be rapidly inhibited by small, cell-permeable drugs like 1-Na PP1 (4-amino-1-tert-butyl-3-(1'-naphthyl)pyrazolo[3,4-d]pyrimidine).
Loss of kinase activity. Abolishes interaction to PHO80 and PCL1 cyclins.
Reduces kinase activity. Abolishes interaction to PHO80 cyclin, but not to PCL1.
Altered inheritance of mitochondria protein 32
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
Almost completely abolishes polyphosphatase activity.
Altered inheritance of mitochondria protein 4
2-fold increase in steepness of voltage dependence.
2.5-fold reduction in steepness of voltage dependence.
Mitochondrial outer membrane protein porin 1
Uncharacterized ATP-dependent helicase IRC20
2-acyl-1-lysophosphatidylinositol acyltransferase
Decreased activation of Hsp90 ATPase activity with a small reduction in Hsp90 binding affinity.
Decreases activation of Hsp90 ATPase activity and substantially reduces Hsp90 binding affinity.
Decreases activation of Hsp90 ATPase activity with a small reduction in Hsp90 binding affinity.
No effect on Hsp90 ATPase activity but reduces Hsp90 binding affinity.
Vacuolar protein sorting-associated protein IST1
Impairs the import of mitochondrial carrier proteins into mitochondria.
In tim9-19; impairs the import of mitochondrial carrier proteins into mitochondria.
In tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with A-40.
In tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with P-60.
Mitochondrial import inner membrane translocase subunit TIM9
Decreases Mg(2+) import into mitochondria.
Increases Mg(2+) import into mitochondria.
Magnesium transporter MRS2, mitochondrial
Uncharacterized membrane glycoprotein YNR066C
Mediator of RNA polymerase II transcription subunit 4
Gives rise to unstable commitment complexes.
Completely abolishes interaction with PCF11. No effect on growth; when associated with A-136.
Completely abolishes interaction with PCF11. No effect on growth; when associated with A-137.
Compromises interaction with PCF11. No effect on growth.
mRNA cleavage and polyadenylation factor CLP1
Chromosome segregation in meiosis protein 4
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
Single-stranded nucleic acid-binding protein
In SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-483. In SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with 477-AA-478.
In SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-487.
In SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with A-487.
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1
Severe growth defect; lowers SRP-dependent and SRP-independent translocation.
Severe growth defect; lowers SRP-dependent translocation.
DNA topoisomerase 2-associated protein PAT1
Loss of function. Localizes correctly to the mitochondrion, but reduces the steady-state level of the protein in the mitochondrial fraction.
Loss of function. Localizes correctly to the mitochondrion, but severely reduces the steady-state level of the protein in the mitochondrial fraction.
Peroxisomal acyl-coenzyme A thioester hydrolase 1
Ribonucleases P/MRP protein subunit POP8
In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72.
In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155.
In MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155.
In MSL5-5; cold-sensitive; growth defect; when associated with D-195.
In MSL5-5; cold-sensitive; growth defect; when associated with V-258.
Transcription factor IIIB 70 kDa subunit
Probable metabolite transport protein YBR241C
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; G-103 and S-219.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; G-103 and V-175.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; V-175 and S-219.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with G-103; V-175 and S-219.
Constitutively active allele, bypass the tRNA binding-requirement for kinase activity.
Does not abolish derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells.
Fails to derepress of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells and does not inhibit autophosphorylation.
Increases tRNA binding and the constitutive kinase activity and induces derepression of GCN4 translation and amino acid biosynthetic genes in absence of amino acid starvation.
Increases the constitutive kinase activity and induces derepression of GCN4 translation and amino acid biosynthetic genes in absence of amino acid starvation.
Increases the constitutive kinase activity in absence of amino acid starvation.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation in amino acid-starved cells.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation. Restores eIF-2-alpha kinase activity and derepression of GCN4, but not autophosphorylation; when associated with D-594.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation. Restores eIF-2-alpha kinase activity and derepression of GCN4, but not autophosphorylation; when associated with R-598.
Inhibits binding to uncharged tRNAs, decreases eIF-2-alpha kinase activity and derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells; when associated with L-1119.
Inhibits binding to uncharged tRNAs, decreases eIF-2-alpha kinase activity and derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells; when associated with L-1120.
Inhibits dimerization; when associated with A-1080 and A-1088.
Inhibits dimerization; when associated with A-1080 and A-1090.
Inhibits dimerization; when associated with A-1088 and A-1090.
Inhibits interaction with GCN1, eIF-2-alpha phosphorylation and fails to derepress GCN4 translation in amino acid-starved cells.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with I-1553 and I-1556.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with L-1552 and I-1553.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with L-1552 and I-1556.
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1
Impairs BIR1 degradation and death-promoting activity.
12 X 28 AA tandem repeats of S-[AV]-[P]-V-A-V-S-S-T-Y-T-S-S-P-S-A-P-A-A-I-S-S-T-Y-T-S-S-P
Altered inheritance of mitochondria protein 39, mitochondrial
Putative uncharacterized protein YDL007C-A
Peroxisomal coenzyme A diphosphatase 1, peroxisomal
Class E vacuolar protein-sorting machinery protein HSE1
Carnitine O-acetyltransferase, mitochondrial
DNA-directed RNA polymerase I subunit RPA34
Decreased ability to prevent stalled ribosomes from frameshifting.
Decreased interaction with the ribosome.
Does not affect ability to prevent stalled ribosomes from frameshifting.
Slightly decreased interaction with the ribosome.
Trafficking protein particle complex subunit 31
Abolished E3 ubiquitin-protein ligase activity.
Mitochondrial FAD-linked sulfhydryl oxidase ERV1
In pmr3-1; decreases nuclear fusion efficiency.
In pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises.
In pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency.
Required for respiratory growth protein 1, mitochondrial
Pentatricopeptide repeat-containing protein PET309, mitochondrial
Methylenetetrahydrofolate dehydrogenase [NAD(+)]
No TPx activity, no effect on DOT activity.
U6 snRNA-associated Sm-like protein LSm6
DNA-directed RNA polymerases I, II, and III subunit RPABC4
eRF1 methyltransferase catalytic subunit MTQ2
Alters cofactor preference of the enzyme to be able to use as well NAD instead of NADP.
NADPH-dependent methylglyoxal reductase GRE2
Probable pyridoxal 5'-phosphate synthase subunit SNZ2
Greatly diminishes transcriptional activation.
Probably encodes all the information necessary to anchor HAP4 to HAP2/3
Actin-related protein 2/3 complex subunit 3
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Uncharacterized peptide chain release factor-like protein YLR281C, mitochondrial
Actin-related protein 2/3 complex subunit 5
Uncharacterized membrane protein YFL067W
Vacuolar cation-transporting ATPase YPK9
Probable serine/threonine-protein kinase IKS1
DNA damage checkpoint control protein RAD17
Decreases catalytic activity 5-fold, but retains substrate binding properties.
In ram2-1; completely abolishes farnesyltransferase activity, but still has about 30% geranyl-geranyl transferase activity.
Increases KM for peptide substrate 4-fold, but retains catalytic activity.
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
DNA-directed RNA polymerase III subunit RPC2
Dual-specificity protein phosphatase SDP1
Maltose fermentation regulatory protein MAL13
56 kDa U1 small nuclear ribonucleoprotein component
DNA repair/transcription protein MET18/MMS19
C-1-tetrahydrofolate synthase, cytoplasmic
Cyclohydrolase activity is increased 2-fold. Dehydrogenase Km is increased 2-fold.
Cyclohydrolase activity is reduced 20-fold. Dehydrogenase Km is increased 7-fold.
Methylenetetrahydrofolate dehydrogenase and cyclohydrolase
Bifunctional purine biosynthesis protein ADE16
ERAD-associated E3 ubiquitin-protein ligase HRD1
Stabilizes HRD1. Reduced interaction with substrate. Formation of oligomer with or without USA1.
Signal recognition particle subunit SRP14
Signal recognition particle receptor subunit alpha homolog
Trafficking protein particle complex subunit 33
Causes respiratory deficiency in absence of RSM28.
Abolished ability to cleave polypeptidyl-tRNAs to release incompletely synthesized polypeptides from 60S ribosomal subunit.
Abolished ability to release incompletely synthesized polypeptides from 60S ribosomal subunit.
Does not affect ability to release incompletely synthesized polypeptides from 60S ribosomal subunit.
Reduced ability to release incompletely synthesized polypeptides from 60S ribosomal subunit.
5'-AMP-activated protein kinase subunit gamma
Decreases SNF1-activation efficiency; when associated with A-291 and E-293.
Decreases SNF1-activation efficiency; when associated with E-242 and A-291.
Decreases SNF1-activation efficiency; when associated with E-242 and E-293.
Reduces glucose inhibition of SNF1 and leads to resistance to 2-deoxyglucose.
In RGT2-1; constitutively signaling glucose receptor.
In Rgt2(4SA); Abolishes Yck-dependent phosphorylation, interaction with MTH1 and STD1, and glucose signaling, but does not affect protein stability.
Uncharacterized endoplasmic reticulum membrane protein YMR122W-A
Prevents phosphorylation; when associated with A-15 and A-19.
Prevents phosphorylation; when associated with A-15 and A-235.
Prevents phosphorylation; when associated with A-19 and A-235.
18 X 45 AA approximate tandem repeats, Thr-rich
2 X 20 AA approximate tandem repeats, Ser/Thr-rich
3 X 9 AA approximate tandem repeats, Thr-rich
Cytochrome c1, heme protein, mitochondrial
Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-289.
Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-296.
Loss of CYT1 and COB from the bc1 complex; when associated with L-289 and L-296.
Endoplasmic reticulum junction formation protein lunapark
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-226 and A-244.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-226 and A-247.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-244 and A-247.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-226, A-244 and A-247.
Chromatin-remodeling complex subunit IES6
Meiosis induction protein kinase IME2/SME1
External NADH-ubiquinone oxidoreductase 2, mitochondrial
Extender of the chronological lifespan protein 1
Decreases nucleotide binding by a factor of 2.5 and reduces catalytic activity. Decreases nucleotide binding by a factor of 20 and abolishes catalytic activity; when associated with A-266.
Decreases nucleotide binding by a factor of 4 and reduces catalytic activity. Decreases nucleotide binding by a factor of 20 and abolishes catalytic activity; when associated with A-264.
Reduces ATP turnover and polyP synthesis.
Vacuolar transporter chaperone complex subunit 4
Blocks Ca(2+)-stimulated auto-processing and its catalytic activity towards substrates.
No effect on auto-processing and catalytic activity towards substrates.
Prevents auto-cleavage at Arg-72 but not at Lys-86. Does not affect catalytic activity; when associated with A-86.
Prevents auto-cleavage at Lys-86 but not at Arg-72. Does not affect catalytic activity; when associated with A-72.
Putative protein lysine methyltransferase SET5
2-isopropylmalate synthase 2, mitochondrial
Mediator of RNA polymerase II transcription subunit 6
Abolishes interaction with PCNA (POL30), but only causes a moderate mismatch repair defect. Partially functional in a mismatch repair assay; when associated with 26-AA-27.
Defective in repairing 8-oxo-G-A mismatches.
Has no defect in mismatch DNA binding, but lacks ATP-induced conformational change.
Impairs ATP binding; reduces catalytic activity 13-fold for ATP hydrolysis.
In MSH6-2; defective for ATP-induced sliding clamp formation, but assembles ternary complexes with MutL alpha.
In MSH6-340; shows defects in mispair DNA binding, but not in homoduplex DNA binding.
In MSH6-3; defective for ATP-induced sliding clamp formation and assembly of ternary complexes with MutL alpha.
In MSH6-4; defective for ATP-induced sliding clamp formation and assembly of ternary complexes with MutL alpha.
In MSH6-5; partially functional in a mismatch repair assay.
In MSH6-6; partially functional in a mismatch repair assay.
In MSH6-7; partially functional in a mismatch repair assay.
In MSH6-9; shows normal mispair binding and dissociation, but fails to show complete mispair activation of the ATPase.
In PMS3-1; completely abolishes mismatch repair.
Partially functional in a mismatch repair assay.
Partially functional in a mismatch repair assay; when associated with 33-AA-34.
Reduces catalytic activity 13-fold for ATP hydrolysis.
Shows defects in both homoduplex and mispair DNA binding and is only partially functional in a mismatch repair assay.
Superoxide dismutase [Mn], mitochondrial
Significantly low viability and relatively normal Ca(2+) accumulation.
Substitution by hydrophilic amino acids causes lethality and low Ca(2+) accumulation.
Substitution by hydrophobic, large amino acids does not cause lethality nor low Ca(2+) accumulation.
4 X 6 AA approximate tandem repeats of A-[SP]-K-K-E-[AV]
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
Genetic interactor of prohibitin 5, mitochondrial
Mediator of RNA polymerase II transcription subunit 7
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-149 in ATG5)
Impairs conjugation to ATG5 and autophagic activity.
Strong decrease of conjugation with ATG5.
Abolishes formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
Interchain (with C-48 in TSA1); transient
Minor effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
No effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
Protein-S-isoprenylcysteine O-methyltransferase
Bifunctional purine biosynthetic protein ADE5,7
In strain: CLIB 556 haplotype Ha2 and CLIB 630 haplotype Ha2.
In strain: CLIB 95, CLIB 382, CLIB 388, CLIB 556 haplotype Ha2, CLIB 630, K1, R12, R13, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1.
N-(5'-phosphoribosyl)anthranilate isomerase
Near wild-type activity for deacetylation. Increases slightly the KM for NAD(+) to 25 uM.
Nearly or completely catalytically inactive.
Alpha1-proteinase inhibitor-degradation deficient protein 37
Mitochondrial inner membrane protease subunit 2
Reduces processing of CYB2 and presence of IMP1 and SOM1 in the IMP complex.
Reduces processing of cytochrome c1; no effect on presence of IMP1 and SOM1 in the IMP complex.
Medium-chain fatty acid ethyl ester synthase/esterase 2
Interaction with isopentenylpyrophosphate transferase
Leads to a defect in complex IV assembly and subsequent respiratory deficiency.
Leads to a defect in complex V assembly and subsequent respiratory deficiency.
Leads to a defect in complexes IV and V assembly, and subsequent respiratory deficiency.
Mitochondrial inner membrane protein OXA1
In TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations.
Transcription factor tau 138 kDa subunit
In A364a; suppressor of the cdc35-1 allele.
In sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213.
In sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213.
In sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99.
In sgt1-5; induces arrest of cell division in G2/M; when associated with K-364.
In sgt1-5; induces arrest of cell division in G2/M; when associated with V-220.
N-acetyl-gamma-glutamyl-phosphate reductase
Putative uncharacterized protein YER076C
Contains the major tRNA-Phe binding sites
Phenylalanine--tRNA ligase alpha subunit
No defect in the interaction with CDC48.
Chromatin structure-remodeling complex subunit RSC1
Mitochondrial outer membrane protein OM45
DNA mismatch repair protein MSH1, mitochondrial
Increases the rate of point mutations 19-fold and displays a moderate decrease in respiration-competent cells during a time course.
Increases the rate of point mutations 35-fold and displays a significant decrease in respiration-competent cells during a time course.
Slightly increases the frequency of point mutations and repeat-mediated deletion rates in mtDNA.
Leads to a mild increase in the proportion of preanaphase spindles at the expense of elongated spindles.
Completely inactivates the enzyme. Resistance to sordarin.
Y' element ATP-dependent helicase YEL077C
Vacuolar protein sorting-associated protein 38
Tyrosine-protein phosphatase-like protein OCA2
Almost complete loss of secreted alpha-agglutinin activity by 10-fold.
Almost complete loss of secreted alpha-agglutinin activity.
Complete loss of alpha-agglutinin activity.
Decreases secreted alpha-agglutinin activity by 10-fold.
Decreases secreted alpha-agglutinin activity by 100-fold.
Decreases secreted alpha-agglutinin activity by 15-fold.
Decreases secreted alpha-agglutinin activity by 2-fold.
Decreases secreted alpha-agglutinin activity by 4-fold.
Decreases secreted alpha-agglutinin activity by 60-fold.
Decreases secreted alpha-agglutinin activity by less than 2-fold.
Decreases secreted alpha-agglutinin activity by more than 20-fold.
Ig-like fold domain important for alpha-agglutinin activity, contributing to a functional binding site for a-agglutinin
Little effect on secreted alpha-agglutinin activity.
Little or no effect on secreted alpha-agglutinin activity.
No effect on secreted alpha-agglutinin activity.
U3 small nucleolar RNA-associated protein 20
Uncharacterized membrane protein YGL140C
Small nuclear ribonucleoprotein-associated protein B
Adenylate kinase isoenzyme 6 homolog FAP7
Decreases the processing of 20S rRNA; when associated with A-82.
Decreases the processing of 20S rRNA; when associated with A-84.
Reduces kcat 100-fold. Reduces substrate affinity 900-fold.
Reduces kcat over 100000-fold. Reduces substrate affinity 11-fold.
Peptide methionine sulfoxide reductase 2
Altered inheritance of mitochondria protein 23, mitochondrial
Impairs basic amino-acids transport and regulation by these amino-acids.
Osmolarity two-component system protein SSK1
Ribonucleoside-diphosphate reductase small chain 2
Translation machinery-associated protein 46
Transcription initiation factor TFIID subunit 13
Has weaker affinity for S-adenosyl-L-methionine and reduces catalytic activity by 90%.
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5
Attenuates the coordination of ion and is unable to sustain growth in thiamine-free medium.
This mass is that of the untagged protein plus the mass of an iron ion.
Unable to sustain growth in thiamine-free medium.
ATPase synthesis protein 25, mitochondrial
Diadenosine 5',5""'-P1,P4-tetraphosphate phosphorylase 2
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336.
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-336.
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-474. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-417 or S-434.
Multiple RNA-binding domain-containing protein 1
In got1-1; loss of function. ER-Golgi transport defect when associated with lack of SFT2.
Abolished E3 ubiquitin-protein ligase activity, leading to impaired peroxisome biogenesis.
Abolished interaction with PEX12, leading to prevent E3 ubiquitin-protein ligase activity.
Reduced E3 ubiquitin-protein ligase activity.
Eukaryotic translation initiation factor 3 subunit I
In FIG4-1; loss of catalytic activity in vitro. Almost 3-fold increase in PtdIns(3,5)P2 level in vivo. Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover.
Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover.
TPR repeat-containing protein associated with Hsp90
Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin.
Uncharacterized globin-like protein YNL234W
U4/U6 small nuclear ribonucleoprotein PRP3
Eukaryotic translation initiation factor 4B
ADP-ribosylation factor GTPase-activating protein effector protein 2
Uncharacterized membrane protein YJR124C
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
SWI/SNF global transcription activator complex subunit SWP82
tRNA-splicing endonuclease subunit SEN15
Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-36 and R-37.
Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-61 and R-62.
Actin-related protein 2/3 complex subunit 2
Membrane-anchored lipid-binding protein LAM4
Abolishes ATPase activity and leads to cold sensitivity.
Decreases phosphatidylserine flippase activity and decreases interaction with GEA2. Sensitive to cold.
Decreases phosphatidylserine flippase activity. No effect on interaction with GEA2. Sensitive to cold.
Involved in phosphatidylserine substrate recognition
Loss of activity. Does not appear to reduce interaction with CDC50. Sensitive to cold.
Loss of activity. Sensitive to cinnamycin (phosphatidylethanolamine-binding cytoxin).
Loss of activity. Sensitive to papuamide B (phosphatidylserine-binding cytotoxin); the effect is suppressed when associated with S-445.
No sensitivity to papuamide B (phosphatidylserine-binding cytotoxin) or cold. No sensitivity to papuamide B; when associated with 237-G--A-238 or K-473.
Normal ATPase activity. No sensitivity to cold.
Reduces interaction with CDC50. Sensitive to cold.
Sensitive to cold. Reduces interaction with CDC50. Decreases protein level.
Sensitive to duramycin (phosphatidylethanolamine-binding cytoxin). Decreases ATPase activity.
Sensitive to papuamide B (phosphatidylserine-binding cytotoxin).
Sensitive to papuamide B (phosphatidylserine-binding cytotoxin); the effect is suppressed when associated with S-445.
In allele MUS81-DD; abrogates endonuclease activity.
Ubiquitin carboxyl-terminal hydrolase 13
Confers UV-radiation sensitivity; when associated with F-48 and F-87.
Confers UV-radiation sensitivity; when associated with F-48 and S-88.
Confers UV-radiation sensitivity; when associated with F-87 and S-88.
Impairs ubiquitin conjugation, DNA double-strand brakes formation during meiosis and histone H3-K79 methylation.
5-aminolevulinate synthase, mitochondrial
Lethal in combination with a MCX1 disruption.
Abolishes interaction with OST3 and STT3; disrupts interaction between OST3 and STT3.
Disrupts interaction between OST3 and STT3.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4
No effect on interaction between OST3 and STT3.
Severe growth defect; abolishes interaction with OST3 and STT3.
Severe growth defect; abolishes interaction with OST3.
Severe growth defect; abolishes interaction with OST3; disrupts interaction between OST3 and STT3.
26S proteasome regulatory subunit 8 homolog
Meiosis-specific serine/threonine-protein kinase MEK1
DNA-directed RNA polymerase II subunit RPB11
Transcript termination readthrough. Lethal.
In CNS1-1; disrupts interaction with Hsp90, temperature-sensitive defect impairing Hsp90-dependent function.
In CNS1-2; disrupts interaction with Hsp90, temperature-sensitive defect impairing Hsp90-dependent function.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-260 and G-324.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-260 and S-330.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-324 and S-330.
Chromatin structure-remodeling complex protein RSC8
Uncharacterized transcriptional regulatory protein YKL222C
Important for DNA bending; intercalates between base pairs of target DNA
Reduced enzyme activity; abolishes stimulation of ATPase activity upon DNA binding.
Strongly reduced enzyme activity; abolishes stimulation of ATPase activity upon DNA binding.
Succinate-semialdehyde dehydrogenase [NADP(+)]
Putative uncharacterized protein YDR034C-A
Cytochrome b-c1 complex subunit 10, mitochondrial
In USE1-0; slows down protein transport from the endoplasmic reticulum to the Golgi at 37 degrees Celsius.
Trehalose synthase complex regulatory subunit TPS3
Eukaryotic translation initiation factor 6
Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-251.
Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-254.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis. Alters complex assembly; when associated with A-219.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-266.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-269.
Lethal. Strongly reduces interaction with JNM1.
Lethal. Strongly reduces interaction with JNM1; when associated with A-133.
Lethal. Strongly reduces interaction with JNM1; when associated with A-136.
Lethal. Strongly reduces interaction with JNM1; when associated with A-369.
Lethal. Strongly reduces interaction with JNM1; when associated with A-371.
Lethal; when associated with 214-A--A-216.
Reduces nuclear migration in mitosis; when associated with 368-A-A-369.
Reduces nuclear migration in mitosis; when associated with A-294 and A-296.
Reduces nuclear migration in mitosis; when associated with A-294 and A-298.
Reduces nuclear migration in mitosis; when associated with A-296 and A-298.
Reduces nuclear migration in mitosis; when associated with A-371.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with 84-A-A-85.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-162.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-165.
Reduces spindle formation. Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-87.
Reduces spindle formation; when associated with A-73.
Reduces spindle formation; when associated with A-75.
Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with 48-A-A-49.
Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-46.
Strongly reduces interaction with JNM1; when associated with A-336.
Strongly reduces interaction with JNM1; when associated with A-338.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with 374-A-A-375.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-326.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-328.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-378.
Temperature-sensitive. Strongly reduces interaction with JNM1.
Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-185.
Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-187.
Temperature-sensitive; when associated with A-2.
Temperature-sensitive; when associated with A-6.
Abolishes catalytic activity, but preserves its nonkinase transcription regulation functions.
Abolished protein import in peroxisomes.
Does not affect assembly of the PEX2-PEX10-PEX12 complex.
Does not affect assembly of the PEX2-PEX10-PEX12 complex; causes peroxisomal import defects when associated with mutations in PEX10.
Uncharacterized membrane protein YHR175W-A
Putative redox protein FMP46, mitochondrial
Mitochondrial outer membrane protein porin 2
tRNA threonylcarbamoyladenosine dehydratase 2
Strongly reduces affinity for poly-URNA ends.
U6 snRNA-associated Sm-like protein LSm2
Probable E3 ubiquitin-protein ligase HUL4
N-alpha-acetyltransferase 35, NatC auxiliary subunit
Abolishes the interaction with PRP18, and temperature sensitive growth defect.
Abolishes the interaction with PRP18, and temperature sensitive growth defect. Loss of growth and in vitro splicing activity; when associated with A-122 or A-130 or A-135.
Abolishes the interaction with PRP18. Cryo- and thermosensitivity; when associated with A-122 or A-130 or A-135.
Affects the ability to associate with the spliceosome. Loss of growth and in vitro splicing activity; when associated with 215-AAA-217. Cryo- and thermosensitivity; when associated with A-217.
Affects the ability to associate with the spliceosome. Loss of growth, spliceosome binding, and in vitro splicing activity; when associated with 215-AAA-217. Cryo- and thermosensitivity; when associated with A-217.
Thiamine-repressible mitochondrial transport protein THI74
Cell wall integrity and stress response component 2
In DBP5-1; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-220.
In DBP5-1; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with S-466.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with L-236 and F-245.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-171 and F-245.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-171 and L-236.
In RAT8-2; accumulates poly(A)+ RNA in the nucleus at 16 and 37 degrees Celsius.
In RAT8-3; accumulates poly(A)+ RNA in the nucleus at 16 and 37 degrees Celsius.
In RAT8-7; accumulates poly(A)+ RNA in the nucleus at 16 degrees Celsius.
In SPP91-1; corrects the PRP9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype.
Cleavage of initiator methionine, acetylation of Ala-2 by NatA, slightly reduced acetylation levels but no significant effect on endogenous stability or ability to degrade CPY*.
Degradation in the endoplasmic reticulum protein 1
In der1-2; impairs the ability to degrade misfolded proteins.
N-terminus not predicted to be acetylated. Strongly decreases endogenous stability.
Predicted to lead to acetylation by NatC. No effect on endogenous stability or ability to degrade CPY*.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-331 and A-540.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-331 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-540 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-331; A-540 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-327; A-331; A-540 and A-541. In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-298; D-299; A-313; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; A-313; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; A-324 and D-327.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; A-324 and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; D-299; A-313; A-324; D-327; and D-331.
PMA1 stabilization in the Golgi protein 1
Completely abolishes the enzymatic activity.
Phosphatidate cytidylyltransferase, mitochondrial
Abolishes ARP2/3 complex activation, but not actin binding.
In ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-21.
In ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-24.
In ABP1-2; strongly reduces actin binding and abolishes ARP2/3 complex activation.
In ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-94.
In ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-96.
In ABP1-4; no effect; when associated with A-122.
In ABP1-4; no effect; when associated with A-125.
In ABP1-5; moderately reduces actin binding and abolishes ARP2/3 complex activation.
Temperature-sensitive. TFCIII-DNA complexes present a shift in their 5' border, generate slow-migrating TFIIIB-DNA complexes upon stripping TFIIIC by heparin or heat treatment, and allow initiation at downstream sites. TFIIIC-DNA complexes highly unstable at high temperatures.
No ATPase activity; increase in chromosome missegregation.
Uncharacterized membrane protein YMR253C
Lipid droplet-associated triacylglycerol lipase
Abolishes interaction with LAS17 and causes severe mislocalization of the protein.
Has a constitutive higher activity in actin assembly.
Reduces kcat over 100000-fold. Abolishes of the proton exchange reaction that initiates the enzymatic reaction.
2-deoxyglucose-6-phosphate phosphatase 1
Eukaryotic translation initiation factor 3 subunit G
RNA polymerase I-specific transcription initiation factor RRN7
U6 snRNA-associated Sm-like protein LSm3
Does not affect cell wall incorporation.
Results in complete loss of cell wall incorporation.
Results in the incorporation of KEX2-unprocessed precursor protein into the cell wall.
Ubiquitin carboxyl-terminal hydrolase 16
Cytoplasmic 60S subunit biogenesis factor REH1
Probable alpha-1,3-mannosyltransferase MNT4
Signal recognition particle subunit SRP72
3-ketodihydrosphingosine reductase TSC10
Mitochondrial import receptor subunit TOM22
Cytochrome oxidase assembly protein SHY1
Abolished binding to acetylated histone H3.
Abolished binding to acylated histone H3.
Does not affect ability to bind crotonylated lysines, while abolishing binding to acetylated lysines.
Does not affect binding to acylated histone H3.
Transcription initiation factor TFIID subunit 14
Endosomal/vacuolar adapter protein YPT35
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Maltose fermentation regulatory protein YPR196W
DNA-directed RNA polymerase III subunit RPC1
In FAM1-1; extragenic suppressor mutation, which creates a new initiation codon and adds 18 amino acids to the N-terminus of the protein. This extension has all the characteristics of a mitochondrial targeting sequence and the FAM1-1 mutant protein is indeed imported into the mitochondria, where it restores respiratory growth to a deletion of the mitochondrial beta-keto-acyl synthase CEM1.
Impairs association with the nuclear pores and interaction with MTR2.
Structural maintenance of chromosomes protein 3
Abolishes interaction with BUB3. Benomyl-sensitive phenotype.
Abolishes interaction with CDC20. Benomyl-sensitive phenotype.
Spindle assembly checkpoint component MAD3
Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-78.
Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-81.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6
Ribonuclease P protein component, mitochondrial
Loss of kinase activity and no vacuolar carboxypeptidase Y (PCR1) sorting to the vacuole.
In strain: ABYS 60, DBVPG6044, SK1 and YPS128.
Cytochrome B translational activator protein CBS2
May provide specificity for triple-helical beta-glucan
Nonsense-mediated mRNA decay factor EBS1
1-acyl-sn-glycerol-3-phosphate acyltransferase
Mediator of RNA polymerase II transcription subunit 19
Glycylpeptide N-tetradecanoyltransferase
In NMT1-181; temperature-sensitive with myristic acid auxotrophy. Reduces affinity for myristoyl-CoA.
In NMT1-72; temperature-sensitive mutant with myristic acid auxotrophy.
Moderately reduces affinity for myristoyl-CoA, but not for substrate.
Moderately reduces affinity for substrate, but not for myristoyl-CoA.
Reduces affinity for both substrate and myristoyl-CoA.
Reduces affinity for myristoyl-CoA, but not for substrate.
Reduces affinity for substrate, but not for myristoyl-CoA.
Reduces chemical transformation rate 400-fold.
Reduces the chemical transformation rate; when associated with A-170.
Reduces the chemical transformation rate; when associated with A-171.
Reduces the chemical transformation rate; when associated with A-205.
Reduces the chemical transformation rate; when associated with L-169.
Abolishes transcriptional activation of target genes.
Reduces transcriptional activation of target genes.
pH-response transcription factor pacC/RIM101
Altered inheritance of mitochondria protein 26, mitochondrial
In dsl1-22; strong Golgi-ER retrograde transport defect.
Loss of interaction with RET1; when associated with A-413.
Viable and no effect. Lethal and loss of interaction with RET2 and reduced interaction with RET1; when associated with A-413.
Viable and reduced interaction with RET2, strong Golgi-ER retrograde transport defect. Loss of interaction with RET2; when associated with A-455. Lethal and loss of interactions with RET1 and RET2; when associated with A-425.
In pol3-13; synthetically lethal with mutations of NBP35, DRE2 and TAH18.
Actin-related protein 2/3 complex subunit 1
General transcription and DNA repair factor IIH subunit TFB2
Putative alanyl-tRNA editing protein alaX
Styryl dye vacuolar localization protein 3
RNA polymerase II subunit A C-terminal domain phosphatase
10 kDa heat shock protein, mitochondrial
Only moderately reduces the thioesterase activity.
Mitochondrial peculiar membrane protein 1
Uncharacterized membrane protein YMR155W
DNA-directed RNA polymerase II subunit RPB4
GTP:AMP phosphotransferase, mitochondrial
Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs).
Probable serine/threonine-protein kinase HSL1
Mediator of RNA polymerase II transcription subunit 2
Reduces expression of several genes from the endogenous 2-micron plasmid and augments expression of numerous iron-response genes.
Probable vacuolar amino acid transporter YPQ3
Probable metabolite transport protein YFL040W
Cytochrome c oxidase subunit 8, mitochondrial
Eukaryotic translation initiation factor 5
In TIF5-12A; Abolishes binding to SUI3 and NIP1.
In TIF5-7A; Abolishes binding to SUI3 and NIP1.
Mitochondrial 2-methylisocitrate lyase ICL2
Hydroxyacyl-thioester dehydratase type 2, mitochondrial
Abolishes fusion function, MDM30-binding and MDM30-dependent ubiquitination, but not localization to mitochondrial outer membrane.
Abolishes fusion function, MDM30-binding and MDM30-dependent ubiquitination. No effect on localization or interaction with UGO1.
Abolishes fusion function; when associated with Ala-501.
Abolishes fusion function; when associated with Ala-504.
Abolishes respiratory growth. Restores respiratory growth; when associated with D-464.
Abolishes respiratory growth. Restores respiratory growth; when associated with D-780.
Abolishes respiratory growth. Restores respiratory growth; when associated with K-335.
Impairs GTPHydrolysis and abolishes fusion function.
Leads to an unusual intermediate mitochondrial morphology described as disorganized tubules in which mitochondria are tubular, distorted, less branched, and poorly distributed throughout the cell.
No effect. Restores respiratory growth; when associated with H-523.
Abolishes proteolytic processing of ATP6, but still promotes assembly of the ATP6 precursor into the ATPase CF(0) particle.
Mitochondrial inner membrane protease ATP23
Phosphopantothenoylcysteine decarboxylase subunit SIS2
Uncharacterized vacuolar membrane protein YNL115C
Loss of activity. No activation of VPS34 kinase activity.
Modest effect on activity and normal CPY sorting.
No myristoylation, but still membrane-association and normal activity.
Peptidyl-prolyl cis-trans isomerase CPR4
ATP-dependent clpX-like chaperone, mitochondrial
Abrogates unfolding activity of unfoldase. Mildly increased ATPase activity. Reduces ALA synthesis.
Does not affect activity but increases by twofold the amount of protein found in mixed disulfide with PDI1 or MPD2.
Impairs the capture of mixed-disulfide with PDI1 thereby blocking its function. Loss of activity; when associated with A-105.
In ERO1-1; induces defective folding of disulfide proteins.
In ERO1-2; induces defective folding of disulfide proteins.
Loss of activity. Prevents its reoxidation thereby blocking its function.
Loss of regulatory disulfide bond and strongly increased activity towards PDI; when associated with A-150.
Loss of regulatory disulfide bond and strongly increased activity towards PDI; when associated with A-295.
TATA-binding protein-associated factor MOT1
DNA-directed RNA polymerase II subunit RPB7
Lowers nucleic-acid binding of RPB4-RPB7 by 10-fold; no effect on association with Pol II core complex; abolishes transcriptional activity of Pol II.
No effect on nucleic-acid binding of RPB4-RPB7 and on association with Pol II core complex; abolishes transcriptional activity of Pol II.
Putative uncharacterized protein YBR013C
Required for interaction with CDC34/UBC3
Required for interaction with SKP1/CBF3D and F-box protein
Vacuolar protein sorting-associated protein 54
In PRP40-1 suppressor; affects SAR1 mRNA accumulation in U1-U4 mutant at 18 degrees Celsius.
Temperature sensitive growth at 36 degrees Celsius.
Galactose/lactose metabolism regulatory protein GAL80
Ubiquitin carboxyl-terminal hydrolase 14
1-acylglycerol-3-phosphate O-acyltransferase ICT1
Causes partial retention of the protein in the endoplasmic reticulum.
Causes the protein to be retained in the endoplasmic reticulum (ER) and degraded via ER-associated degradation.
Oligomycin resistance ATP-dependent permease YOR1
Prospore formation at selected spindle poles protein 1
Cx9C motif-containing protein 4, mitochondrial
6 X 4 AA tandem repeats of N-[AT]-[NT]-A
O-(3'-phospho-DNA)-tyrosine intermediate
ABC transporter ATP-binding protein/permease VMR1
Sporulation-specific glucan 1,3-beta-glucosidase
Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1
Putative uncharacterized protein YLR462W
Pyridoxamine 5'-phosphate oxidase homolog
Biogenesis of lysosome-related organelles complex 1 subunit BLI1
Dominant negative mutant; prevents the nuclear export of 60S ribosomal subunit in cytoplasm.
Impairs the nuclear export of 60S ribosomal subunit in cytoplasm.
No effect on growth rate. Restores cell growth; when associated with Q-315.
Probable loss of ATP binding. Complete loss of catalytic activity.
Restores cell growth; when associated with Q-315.
Severe loss of catalytic activity and cooperativity between the 2 ATP-binding regions. Lethal. Restores cell growth; when associated with A-358; A-369; S-471; A-471 or H-475.
Severe loss of catalytic activity without affecting cooperativity between the 2 ATP-binding regions. Slight reduction in growth rate.
Severe loss of catalytic activity. Lethal.
Slight reduction in growth rate. Restores cell growth; when associated with Q-315.
Peroxisomal long-chain fatty acid import protein 2
Nitrosoguanidine resistance protein SNG1
Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial
Sterol regulatory element-binding protein ECM22
Uncharacterized deoxyribonuclease YMR262W
Phosphatidylinositol transfer protein CSR1
Thiazolidine linkage to a ring-opened DNA abasic site
GPI ethanolamine phosphate transferase 3
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with P-556; G-887 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; G-887 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; P-556 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; P-556 and G-887.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with R-198 and S-231.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with R-198 and T-411.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with S-231 and T-411.
Ectonucleotide pyrophosphatase/phosphodiesterase 1
In SRB5-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.
Mediator of RNA polymerase II transcription subunit 18
E3 ubiquitin-protein ligase linker protein MMS1
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-40.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-44.
Vacuolar protein sorting-associated protein 28
Impairs the folding and stability of the protein.
Partially impairs the folding and stability of the protein.
91% decrease of DGPP phosphatase activity.
Complete loss of DGPP phosphatase activity.
Diacylglycerol pyrophosphate phosphatase 1
H/ACA ribonucleoprotein complex non-core subunit NAF1
Sorting assembly machinery 37 kDa subunit
DNA replication complex GINS protein SLD5
In sld5-12; temperature-sensitive mutant. Defective in DNA replication.
In sld5-13; temperature-sensitive mutant. Defective in DNA replication.
In sld5-2; temperature-sensitive mutant. Defective in DNA replication.
In sld5-8; temperature-sensitive mutant; in association with P-21. Defective in DNA replication.
In sld5-8; temperature-sensitive mutant; in association with P-66. Defective in DNA replication.
Superficial pseudohyphal growth protein 1
Grand meiotic recombination cluster protein 2
Cytochrome c oxidase assembly protein COX16, mitochondrial
In PCF1-11; increases RNA polymerase III gene transcription.
In PCF1-12; increases RNA polymerase III gene transcription.
In PCF1-138; increases RNA polymerase III gene transcription.
In PCF1-139; increases RNA polymerase III gene transcription.
In PCF1-15; increases RNA polymerase III gene transcription.
In PCF1-17; increases RNA polymerase III gene transcription.
In PCF1-19; increases RNA polymerase III gene transcription.
In PCF1-1; affects the rate of recruitment of TFIIIB to the template. Increases the amount of transcriptionally active TFIIIB. Increases RNA polymerase III gene transcription. Increases the binding affinity for BRF1, but does not affect the binding affinity for BDP1 in the TFIIIC-dependent assembly of TFIIIB. Overcomes autoinhibition of BRF1 binding.
In PCF1-23; increases RNA polymerase III gene transcription.
In PCF1-2; increases RNA polymerase III gene transcription due to an increase in the recruitment of BRF1 to TFIIIC-DNA. No effect on affinity of TFIIIC for DNA.
In PCF1-3; increases RNA polymerase III transcription two- to threefold over wild-type. Increases the amount of transcriptionally active TFIIIB.
In PCF1-4; increases RNA polymerase III transcription ninefold over wild-type. Increases the amount of transcriptionally active TFIIIB.
In PCF1-5; increases RNA polymerase III transcription.
In PCF1-6; increases RNA polymerase III transcription.
In PCF1-7; increases RNA polymerase III transcription.
In PCF1-8; increases RNA polymerase III transcription.
RNA polymerase III defective. Defect in the recruitment of BRF1 into TFIIIB-TFIIIC-DNA complexes and diminished direct interaction between TFC4 and BRF1. Decreased binding affinity for BDP1 incorporation into TFIIIB-TFIIIC-DNA complexes and inhibited binary interaction between BDP1 and TFC4.
Transcription factor tau 131 kDa subunit
Reduced ATPase activity of the INO80 complex.
Cytochrome c oxidase subunit 6, mitochondrial
Abolishes pseudouridylate synthase activity.
Decreased pseudouridylate synthase activity.
Does not affect pseudouridylate synthase activity.
Strongly decreased pseudouridylate synthase activity.
Transcription initiation factor TFIID subunit 8
Guanine nucleotide-binding protein subunit gamma
Vacuolar import and degradation protein 30
ATP-dependent RNA helicase MRH4, mitochondrial
Putative 2-hydroxyacid dehydrogenase YPL113C
Type 2A phosphatase-associated protein 42
Uncharacterized mitochondrial carrier YFR045W
Low-affinity potassium transport protein
Probable pyridoxal 5'-phosphate synthase subunit SNO2
Putative uncharacterized protein YCL002C
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
In strain: YPH499; lack of gamma-glutamyl transferase activity.
Structure-specific endonuclease subunit SLX4
Conserved oligomeric Golgi complex subunit 7
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
18 X approximate tandem repeats, Ser/Thr-rich
Mannosyl phosphorylinositol ceramide synthase SUR1
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Probable UTP--glucose-1-phosphate uridylyltransferase
Genetic interactor of prohibitin 7, mitochondrial
Leads to sensitivity to sertraline, when associated with A-273.
Leads to sensitivity to sertraline, when associated with S-275.
Cytochrome b-c1 complex subunit 8, mitochondrial
In PDC1-8; reduces catalytic activity to 10% but retains autoregulatory activity.
Translation machinery-associated protein 17
Monoisotopic mass with either 7 methylation modifications or 1 acetylation and 4 methylation modifications.
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
Ectonucleotide pyrophosphatase/phosphodiesterase 2
Y' element ATP-dependent helicase YLL066C
26S proteasome regulatory subunit 6B homolog
Fully functional; when associated with deletion of 1-M--K-115.
Fully functional; when associated with deletion of 260-L--T-458.
Eukaryotic translation initiation factor eIF-1
Defective in cullin neddylation protein 1
Branched-chain-amino-acid aminotransferase, mitochondrial
G1-specific transcriptional repressor WHI5
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]
Decreases dissociation constants by 4-fold for NAD(+) and 2-fold for NADH, while turnover numbers were decreased by 4-fold for ethanol oxidation and 6-fold for acetaldehyde reduction.
Has an inverted specificity pattern for primary alcohols, being 3- and 10-fold more active on hexanol and 350- and 540-fold less active on ethanol. Also acquires weak activity on branched chain alcohols and cyclohexanol.
Has lowered reactivity with primary and secondary alcohols.
Has the same pattern of activity as the wild-type enzyme for linear primary alcohols.
Produces a 7 to 10-fold increase in reactivity with butanol, pentanol, and hexanol.
Vacuolar protein sorting-associated protein 8
Eukaryotic translation initiation factor 5A-2
In YIP1-12; abolishes binding to YPT1 and YPT31.
In YIP1-18; abolishes binding to YIF1, YPT1 and YPT31.
In YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1.
In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129.
In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114.
In YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius.
In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70.
In YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130.
In YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding.
In YIP1-44; lethal; when associated with K-70.
In YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130.
In YIP1-6; lethal; reduces binding to YIF1.
Transcriptional regulatory protein PHO23
Mitochondrial import receptor subunit TOM20
H/ACA ribonucleoprotein complex subunit NHP2
Lethal. Impaired association with H/ACA snoRNAs. Accumulation of NHP2 within the nucleolus.
Significant growth impairment at 30 and 37 degrees Celsius. Impaired association with H/ACA snoRNAs.
Proton acceptor; for glutaminase activity
Conserved oligomeric Golgi complex subunit 4
Chromatin structure-remodeling complex subunit RSC7
Functional region; able to complement all NPL6 null allele phenotypes
Vacuolar cation-chloride cotransporter 1
Abolishes interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B.
Abolishes interaction with histones H2A, H2B, H3 and H4.
Important for interaction with heterotetrameric histone H3 and H4 and for interaction with dimeric histone H2A and H2B
Interaction with dimeric histone H2A and H2B
Mildly decreases interaction with heterotetrameric histone H3 and H4 and abolishes interaction with dimeric histone H2A and H2B.
Minimal decrease of interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and A-291.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-291 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-288; A-291 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-250; A-288; A-291 and 332-A--A-342.
Transcription initiation factor TFIID subunit 4
In TSC13-1; reduces fatty acid elongation activity by 50%.
Carbamoyl-phosphate synthase arginine-specific large chain
Serine/threonine-protein phosphatase 4 regulatory subunit 3
Putative uncharacterized helicase-like protein YBL112C
Putative uncharacterized helicase-like protein YHR218W-A
Impairs oligomerization. Reduces ATPase activity by 92%.
Cytosolic Fe-S cluster assembly factor NBP35
Loss fo function and disrupts heterotetramer formation.
Supports growth, albeit at a lower rate.
Alpha-ketoglutarate-dependent sulfonate dioxygenase
Completely abolishes reductase activity.
Ribonucleoside-diphosphate reductase large chain 1
Nonviable. Loss of catalytic activity. Loss of protein stability.
Nonviable. Loss of catalytic activity. No effect on protein stability and interaction with SPC3.
Nonviable. Loss of catalytic activity. No effect on protein stability.
Signal peptidase complex catalytic subunit SEC11
Mitochondrial import inner membrane translocase subunit TIM8
Measured from a recombinant protein expressed in E.coli.
Transcription initiation factor TFIID subunit 1
Zinc-regulated GTPase metalloprotein activator 1
Vacuolar protein sorting-associated protein 17
In SRB7-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.
Mediator of RNA polymerase II transcription subunit 21
Initiation-specific alpha-1,6-mannosyltransferase
Regulator of rDNA transcription protein 15
Cytochrome b translational activator protein CBS1, mitochondrial
In CBS1-1, which is respiratory deficient.
Loss of function, causes respiratory deficiency.
Altered inheritance rate of mitochondria protein 29
In PRP83-2; temperature-sensitive; blocks splicing of RPS17A after a 2 hour shift to the restrictive temperature of 37 degrees Celsius; spliceosome assembly arrested at the complex I stage.
Loss of DNA-binding; when associated with D-383.
Loss of DNA-binding; when associated with D-387.
Abolishes DNA-binding and endonuclease activity.
Leads to camptothecin hypersensitivity and loss of function.
Leads to camptothecin hypersensitivity and loss of function; when associated with A-223.
Leads to camptothecin hypersensitivity and loss of function; when associated with A-225.
Leads to constitutive activation of the DNA repair function.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-278, and A-279. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-249 and A-279.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-278, and A-289. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-249 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-279, and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-278, A-279, and A-289. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-279 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-73, A-75 and A-76. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-249, A-279 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-73, A-75 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-75, A-76 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-73, A-75, A-76 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-73, A-75, A-76 and A-90. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-90, A-249, A-279 and A-289.
Cold-sensitive. Abolishes kinase activity. Delayed growth at early stationary phase. Shows no increase in CTDSer-2 phosphorylation in the transition from rapid growth to stationary phase. Has compromised transcriptional activation of two stationary-phase genes CTT1 and SPI1.
Cold-sensitive. Sensitive to hydroxyurea and UV irradiation. Interferes with ATP-binding.
Impairs ATPase and 5-oxoprolinase activity.
Impairs ATPase and 5-oxoprolinase activity; when associated with A-497.
Impairs ATPase and 5-oxoprolinase activity; when associated with A-498.
In strain: CLIB 219, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha2.
In strain: CLIB 388, CLIB 410, CLIB 556 haplotype Ha2, CLIB 630, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha2.
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13, YIIc12 and YIIc17.
In strain: CLIB 95, CLIB 382, CLIB 556 haplotype Ha1, K1, R12, R13, YIIc12 haplotype Ha1 and YIIc17 haplotype Ha1.
In strain: CLIB 95, CLIB 382, K1, R12, R13, YIIc12 haplotype Ha1 and YIIc17 haplotype Ha1.
Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-27.
Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-28.
Impairs interaction with UPS1 and UPS2; when associated with A-27 and A-28.
Mitochondrial distribution and morphology protein 35
Prevents binding to COX17 mRNA and its rapid decay and increases affinity to HO mRNA, a PUF5 target.
Endoplasmic reticulum membrane protein 65
Abolishes autopalmitoylation and VAC8 palmitoylation.
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial
5-demethoxyubiquinone hydroxylase, mitochondrial
Lacks ubiquinone and accumulates the intermediate DMQH2, causing respiratory deficiency.
Loss of phosphorylation and increased levels of COQ6; when associated with S-20 and S-28.
Loss of phosphorylation and increased levels of COQ6; when associated with S-20 and T-32.
Loss of phosphorylation and increased levels of COQ6; when associated with S-28 and T-32.
ARF guanine-nucleotide exchange factor 2
Abolishes interaction with DRS2 and decreases DRS2 phosphatidylserine flippase activity in the trans-Golgi network membrane. Abnormal secretory vesicle formation.
Histone acetyltransferase type B catalytic subunit
Abolishes binding to acetylated histones.
Does not affect binding to the proteasome.
Does not affect viability in the presence of cycloheximide.
Loss of function; abolishes binding to the proteasome.
DNA-(apurinic or apyrimidinic site) endonuclease 2
Ferric/cupric reductase transmembrane component 7
Transcription initiation factor TFIID subunit 10
BTB/POZ domain-containing protein YLR108C
Ribosome-recycling factor, mitochondrial
H/ACA ribonucleoprotein complex subunit NOP10
UPF0674 endoplasmic reticulum membrane protein YNR021W
In YOS1-1; impairs interaction with YIP1, blocks ER-Golgi protein transport, and causes a severe growth defect at 37 degrees Celsius.
Polyphosphatidylinositol phosphatase INP52
Abolishes cleavage by ESP1 at position R-431; when associated with E-431.
Abolishes cleavage by ESP1 at position R-453.
Reduces cleavage by ESP1 at position R-431. Abolishes cleavage by ESP1 at position R-431; when associated with R-428.
Impairs protein folding and stability. Loss of function.
Putative RNA-binding protein involved in heterochromatin assembly
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Abolishes tyrosine and tryptophan binding, resulting in loss of enzyme activity regulation by effectors.
Constitutively active. Unresponsive to tryptophan activation and tyrosine feedback inhibition.
Constitutively inactive. Unresponsive to tryptophan activation.
Decreases tyrosine and tryptophan binding, resulting in attenuated enzyme activity regulation by effectors.
Decreases tyrosine binding, resulting in loss of enzyme inhibition by tyrosine. Enhances activation by phenylalanine.
Severely decreases tyrosine and tryptophan binding, resulting in loss of enzyme activity regulation by effectors.
60S ribosome subunit biogenesis protein NOP8
In GLC7-1; decreased glycogen accumulation.
Serine/threonine-protein phosphatase PP1-2
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
Arginine metabolism regulation protein II
Abolishes PPIase activity and fails to activate PP2A phosphatases.
Serine/threonine-protein phosphatase 2A activator 1
Abolishes phosphoinositide binding and Golgi localization; when associated with A-178.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-181.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-88.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-97.
Alters phosphorylation but has not effect on Golgi enzymes localization.
Beta-hairpin required for oligomerization
Vacuolar protein sorting-associated protein 74
Probable pyridoxal 5'-phosphate synthase subunit SNZ3
Probable phospholipid-transporting ATPase DNF3
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20
Increases nucleophilicity of active site Cys
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
SWI/SNF chromatin-remodeling complex subunit SNF5
Ran-specific GTPase-activating protein 2
Probable serine/threonine-protein kinase RTK1
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Abolishes cyclic nucleotide phosphodiesterase activity.
Strongly reduces cyclic nucleotide phosphodiesterase activity.
Impairs domerization in the presence of Fe(2+) or a [2Fe-2S] cluster.
Iron-regulated transcriptional activator AFT2
Abolishes both iron-binding and enzyme activity.
Abolishes enzyme activity and impairs iron-binding.
Abolishes iron-binding, substrate-binding, and enzyme activity.
Abolishes substrate-binding and enzyme activity.
Abolishes substrate-binding, enzyme activity, and impairs iron-binding.
Abolishes poly(A) RNA degradation; when associated with A-188.
Abolishes poly(A) RNA degradation; when associated with A-190.
No cell-cycle stop in response to glucose deprivation.
Putative uncharacterized protein YKR104W
Uncharacterized protein YLR283W, mitochondrial
Mediator of RNA polymerase II transcription subunit 16
Causes read-through of both a snoRNA gene terminator and the poly(A) site of a protein-coding gene.
In SEN1-1; gives rise to a temperature-sensitive mutant.
Severely affects activity, especially the second mannosylation step.
Single-strand annealing weakened protein 1
Abolishes endoribonucleolytic activity; no effect on growth. No growth; when associated with N-551.
Exoribonucleolytic activity abolished. Accumulation of partially processed 5.8S rRNA and partially degraded 5' ETS. No growth; when associated with A-171. No growth; when associated with A-198.
Slow growth; when associated with S-47 and S-52.
Slow growth; when associated with S-47 and S-55.
Slow growth; when associated with S-52 and S-55.
Dolichyl-phosphate-mannose--protein mannosyltransferase 5
Morphogenetic regulator of filamentous growth protein 1
Uncharacterized membrane protein YNL033W
Endoplasmic reticulum vesicle protein 25
Topoisomerase I damage affected protein 7
Leads to mislocalization into the cytoplasm.
Strongly reduces phosphorylation by CK2.
Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-746.
Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-748.
Polyphosphatidylinositol phosphatase INP53
Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-424.
Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-427.
Reduces hydrolysis of PtdIns(4)P; when associated with A-424 and A-427.
Serine palmitoyltransferase-regulating protein TSC3
Inner centromere protein-related protein SLI15
Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3.
Probable ATP-dependent RNA helicase DHR2
Putative uncharacterized oxidoreductase YDR541C
Cysteine-rich and transmembrane domain-containing protein YDR034W-B
COX3 mRNA-specific translational activator PET494
Biogenesis of lysosome-related organelles complex 1 subunit VAB2
Mislocalizes to the vacuole membrane and stay associated with the V0 sector after transport out of the ER.
Vacuolar ATPase assembly integral membrane protein VMA21
Mediator of RNA polymerase II transcription subunit 5
N-terminal acetyltransferase B complex catalytic subunit NAT3
Nascent polypeptide-associated complex subunit alpha
Transcription initiation factor IIE subunit alpha
Loss of function. Induces a decrease in transcription activation.
Methylated-DNA--protein-cysteine methyltransferase
G1-specific transcription factors activator MSA1
ATP-dependent permease MDL1, mitochondrial
Decreased release of long peptides from mitochondria.
Lowers histone H2B deubiquitination activity.
Lowers histone H2B deubiquitination activity; when associated with A-46.
Lowers histone H2B deubiquitination activity; when associated with A-49.
Interaction with SRP1 NLS binding site 1
Target of rapamycin complex 2 subunit BIT61
Glutathione-specific gamma-glutamylcyclotransferase
Loss of catalytic activity against glutathione.
Vacuolar protein-sorting-associated protein 25
In SRB4-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.
Mediator of RNA polymerase II transcription subunit 17
RecQ-mediated genome instability protein 1
C-terminal domain (CTD); 24 X 7 AA approximate tandem repeats of Y-S-P-T-S-P-[A-S-N-G]
DNA-directed RNA polymerase II subunit RPB1
Impairs ubiquitination during transcription stress.
Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47.
Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57.
Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47.
Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57.
RNA polymerase I-specific transcription initiation factor RRN9
Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation.
Displays a 60-fold increase in spontaneous mutation accumulation.
Displays an increase in spontaneous mutation accumulation.
In strain: SK1 and YJM320; forms a non-functional heterodimer with MHL1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains.
In strain: SK1, YJM320, YJM339 and YJM421.
Reduces ATPase activity by 62%. Displays 60-fold increase in spontaneous mutation accumulation.
Reduces ATPase activity by 84%. Displays 11000-fold increase in spontaneous mutation accumulation.
Calcium permeable stress-gated cation channel 1
Determines the chain length of the elongated VLCFA
Required for interaction with SPC24-SPC25
U3 small nucleolar RNA-associated protein 6
15 X 12 AA tandem repeats of [SPNAG]-[IL]-[QKNGT]-[PSA]-[QT]-[GQAPISTLYK]-T-G-[YFGML]-[YVMGAQL]-[QVLNPAG]-[ASQPN]
7 X 6 AA tandem repeats of Q-[PS]-T-Q-P-V
8 X 6 AA repeats of [ATVSP]-P-[LVI]-P-[SPQILA]-[VAS]
Actin cytoskeleton-regulatory complex protein PAN1
In RIM20-292; prevents cleavage of RIM101.
pH-response regulator protein palA/RIM20
Abolishes catalytic activity; when associated with E-382.
Abolishes catalytic activity; when associated with R-248.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl; when associated with A-386.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl; when associated with D-388.
Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial
Putative mitochondrial cysteine synthase
RNA polymerase I-specific transcription initiation factor RRN11
ATP synthase assembly factor FMC1, mitochondrial
Lethal(2) giant larvae protein homolog SRO7
Required for interaction with MEX67, SUB2 and THP1
Required for targeting SAC3 to the nuclear pore
Decreases interaction with tubulin heterodimer.
Disrupts interaction with tubulin heterodimer.
Disrupts interaction with tubulin heterodimer. Impairs polymerase activity under microtubule stress.
Disrupts interaction with tubulin heterodimer. No effect on polymerase activity under normal conditions but impairs polymerase activity under microtubule stress.
Impairs polymerase activity under microtubule stress.
No effect on polymerase activity under normal conditions but impairs polymerase activity under microtubule stress.
Low-affinity phosphate transporter PHO91
Presequence translocated-associated motor subunit PAM17, mitochondrial
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
UDP-N-acetylglucosamine pyrophosphorylase
Putative 2-hydroxyacid dehydrogenase YGL185C
Serine/threonine-protein kinase Haspin homolog ALK1
Serine/threonine-protein kinase RIM11/MSD1
Putative uncharacterized protein YCL042W
Calcium/calmodulin-dependent protein kinase I
Abolished ATPase activity of the PEX1-PEX6 AAAATPase complex.
In Amut mutant; abolished ATPase activity of the PEX1-PEX6 AAAATPase complex.
In Bmut mutant; decreased ATPase activity of the PEX1-PEX6 AAAATPase complex.
In PEX1pA2; decreased binding to PEX6. Results in accumulation of PEX5 on peroxisomal membranes.
In PEX1pB2; results in accumulation of PEX5 on peroxisomal membranes.
Deoxyribodipyrimidine photo-lyase, mitochondrial
Electron transfer via tryptophanyl radical
Reduces substrate binding 100-fold. Reduces quantum yield for dimer photolysis 3-fold.
General negative regulator of transcription subunit 3
In DAM1-1; produces abnormal spindles resulting in growth arrest at 34 degrees Celsius.
Lysophosphatidic acid:oleoyl-CoA acyltransferase 1
Mitogen-activated protein kinase SLT2/MPK1
In RMP1-6; temperature-sensitive phenotype. Defective in 5.8S rRNA processing.
U3 small nucleolar RNA-associated protein 25
Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity.
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Loss of function in calcium homeostasis.
In strain: YJM 269, YJM 270, YJM 326 and YJM 1129.
Causes hypersensitivity to DNA-damage-inducing agents and impairs sporulation.
No effect. No effect; when associated with R-124 and R-127.
No effect; when associated with R-120; R-121 and R-124.
No effect; when associated with R-120; R-121 and R-127.
In TEF5-7; reduces translation efficiency and enhances translation fidelity.
Loss of catalytic activity, but still binds to eEF1A.
Reduces translation efficiency and enhances translation fidelity.
Non-structural maintenance of chromosomes element 1
RNA polymerase II subunit B1 CTD phosphatase RTR2
In MYO2-2; causes a vacuole inheritance defect.
In MYO2-338; abolishes interaction with YPT11; when associated with G-1484 and G-1511.
In MYO2-338; abolishes interaction with YPT11; when associated with S-1474 and G-1484.
In MYO2-338; abolishes interaction with YPT11; when associated with S-1474 and G-1511.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; S-1529 and G-1546.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; S-1529 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; S-1529; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; M-1500; S-1529; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with G-1288; M-1500; S-1529; G-1546 and R-1559.
25 X 12 AA tandem repeat of N-[SV]-[RS]-T-[NS]-A-T-T-T-[AE]-[ST]-[IT]
Contains the proteolytic activation cleavage site
Decreases binding to ubiquitin and interaction with ELA1, and decreases polyubiquitination of RPO21 during transcription stress; when associated with 33-A--A-34.
Decreases binding to ubiquitin and interaction with ELA1, and decreases polyubiquitination of RPO21 during transcription stress; when associated with 54-A--A-55.
Loss of autocatalytic cleavage; when associated with F-605.
Loss of autocatalytic cleavage; when associated with L-608.
Loss of autocatalytic cleavage; when associated with P-604.
Loss of autocatalytic cleavage; when associated with R-608.
Abolishes import into mitochondrion; when associated with S-57.
Abolishes import into mitochondrion; when associated with S-61.
Abolishes import into mitochondrion; when associated with S-73.
Abolishes import into mitochondrion; when associated with S-77.
Mitochondrial import inner membrane translocase subunit TIM13
Has defects on both initial ubiquitination and chain elongation of substrate proteins.
Has subtle defects on both initial ubiquitination and chain elongation of substrate proteins.
In RSP5-1; impairs ubiquitin-thioester formation and catalysis of substrate ubiquitination.
Cell wall integrity and stress response component 4
Biogenesis of lysosome-related organelles complex 1 subunit CNL1
Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins.
Does not affect formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins.
Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)
Leads to the loss of catalytic activity.
Thiosulfate:glutathione sulfurtransferase
Disrupts interaction with STE50 and abolishes signal transduction.
Serine/threonine-protein phosphatase 1 regulatory subunit PIG1
N-alpha-acetyltransferase 38, NatC auxiliary subunit
2 X 16 AA repeat of C-X-C-X(4)-C-X-C-X-X-C-X-X-H
Glutathione peroxidase-like peroxiredoxin HYR1
Interchain (with C-598 in YAP1); transient
S-(hydroxymethyl)glutathione dehydrogenase
Maltose fermentation regulatory protein MAL33
Required for vacuolar localization. Mediates aggregation and vesicle formation in Cvt pathway
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at S-43; S-74; S-162; S-360; S-1179 and S-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-236 and A-305.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-127; A-157; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-157; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43; S-74; S-162; S-360; S-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-236 and A-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-127; E-157; E-163; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-236 and E-305. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-236 and E-305.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-236 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-236 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-305 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-236; E-305 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-163; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-157; E-163; E-231; E-236; E-305 and E-320.
In scc2-CE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU); when associated with E-1182.
In scc2-CE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU); when associated with E-1185.
Mimics constitutive phosphorylation and causes inviability through protein instability.
RNA polymerase II holoenzyme cyclin-like subunit
Induces dysregulation of sphingolipid synthesis; when associated with 32-A--A-36 and 51-A--A-53.
Induces dysregulation of sphingolipid synthesis; when associated with A-29 and 32-A--A-36.
Induces dysregulation of sphingolipid synthesis; when associated with A-29 and 51-A--A-53.
Abolishes E3 ubiquitin-protein ligase activity.
Impairs ATG19-binding and autophagic activity.
Impairs ATG19-binding, PE-conjugation, and autophagic activity.
Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-77.
Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-79.
No cleaving of the C-terminal R-117 by ATG4. Defect in Cvt pathway and autophagy.
Phosphatidylethanolamine amidated glycine
Significantly decreases ATG19-binding and autophagic activity.
Eukaryotic initiation factor 4F subunit p130
In TIF4632-233; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation.
Putative uncharacterized protein YLR264C-A
Uncharacterized membrane protein YPL257W
H/ACA ribonucleoprotein complex subunit GAR1
Sugar utilization regulatory protein IMP2
Uncharacterized ATP-dependent helicase IRC5
Probable 26S proteasome regulatory subunit p28
Putative uncharacterized protein YLR053C
Inactivates the proteins ability to provide drug-resistance in vivo.
Inosine-5'-monophosphate dehydrogenase 2
Abolished E3 ubiquitin-protein ligase activity and ability to activate the ribosome quality control (RQC) and No-Go Decay (NGD) pathways.
Required for resistance to killer toxin K28, a protein-toxin encoded by the M28 virus
In strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6044, DBVPG6763, DBVPG6765, SK1, Y55 and YPS128.
In strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1.
Vacuolar protein sorting-associated protein 70
U3 small nucleolar RNA-associated protein 22
Mannan polymerase complexes subunit MNN9
Reduced activity of the M-Pol I complex.
SWI/SNF chromatin-remodeling complex subunit SWI1
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Centromere DNA-binding protein complex CBF3 subunit A
Oligosaccharide translocation protein RFT1
Actin-related protein 2/3 complex subunit 4
Vacuole transporter chaperone complex subunit 5
Trehalose synthase complex regulatory subunit TSL1
Probable pyridoxal 5'-phosphate synthase subunit SNO3
Leucine-rich repeat-containing protein SOG2
3',5'-cyclic-nucleotide phosphodiesterase 2
tRNA-specific adenosine deaminase subunit TAD3
Probable 1,3-beta-glucanosyltransferase GAS3
Does not complement a YME1 deletion mutant, for E541A stabilizes otherwise unstable COX2.
Does not complement a YME1 deletion mutant, for K327R no longer binds or degrades COX2. Probably has no ATPase activity.
Does not complement a YME1 deletion mutant, no longer binds or degrades COX2. Probably has no ATPase activity.
Does not complement a YME1 deletion mutant, retains 20% protease activity in vitro, binds an unfolded hybrid substrate protein.
Does not complement a YME1 deletion mutant.
Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1
Partially complements a YME1 deletion mutant.
No effect on protein abundance. Decreased CAT tail synthesis.
No effect on protein abundance. Decreased CAT tail synthesis. No effect on ribosome-associated ubiquitin-dependent protein catabolic process.
No effect on protein abundance. Loss of CAT tail synthesis. Loss of ribosome-associated ubiquitin-dependent protein catabolic process.
Reduced ability to mediate CAT tailing without affecting binding to 60S ribosome subunits. Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-98.
Reduced ability to mediate CAT tailing without affecting binding to 60S ribosome subunits. Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-99.
Reduced ability to mediate CAT tailing without affecting binding to 60S ribosome subunits. Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-98 and A-99.
Ribosome quality control complex subunit 2
Probable metabolite transport protein YDR387C
Putative uncharacterized transporter YOL162W
Vacuolar protein sorting-associated protein 33
Putative uncharacterized protein YHR045W
U3 small nucleolar RNA-associated protein 13
Abrogates interaction with histone H3 and histone H4 and enhances transcriptional silencing. Abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with E-145 or E-147.
Abrogates interaction with histone H3 and histone H4 and enhances transcriptional silencing. Reduces acetylation of histone H3 on 'K-9' and 'K-56'; when associated with E-145 or E-147.
Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS.
Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with R-48; E-112 or E-147.
Abrogates stimulation of replication-independent chromatin assembly by the HIR complex and abrogates telomeric silencing.
Decreases histone H3/H4 binding affinity.
Enhances transcriptional silencing. Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with R-48; A-112 or E-145.
Impairs interaction with histone H3 and RAD53 and enhances silencing at telomeres and mating-type loci.
Interaction with histone H3, histone H4, RAD53 and the RF-C complex
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with A-145.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with A-147.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with D-94.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with D-96.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to camptothecin.
Reduces interaction with histone H3 and histone H4, enhances transcriptional silencing and reduces transcriptional activation.
Reduces transcriptional silencing; when associated with R-37.
Reduces transcriptional silencing; when associated with R-39.
Dramatically reduces interaction with RGA1.
In CDC42-1; temperature-sensitive mutant.
In oligomycin-resistant mutant and cross-resistance to venturicidin.
In strain: DS400/A3, DS401 and oligomycin-resistant mutant.
Reversibly protonated during proton transport
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
Transcriptional regulatory protein SAP30
Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport
Mediates proton transfer from the protein to the inner aqueous phase
Transcription initiation factor TFIID subunit 2
In mes1; renders the protein unstable in vitro, elevates the KM for methionine in vivo.
Increases ability to transport iron and decreases ability to transport cobalt.
In DSS4-1; a dominant suppressor of SEC4-8.
In aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-7; impairs AAH1 degradation during postdiauxic growth.
Abolishes S-adenosyl-L-methionine binding and methyltransferase activity.
Abolishes methyltransferase activity, but not S-adenosyl-L-methionine binding.
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Required for interaction with nucleosomes and DNA
Necessary for the normal cellular distribution and bud neck targeting
Putative uncharacterized protein YLR030W
2 X 4 AA repeats of S-R-Y-D, N-N-N-Y, Y-S-G-N
Abrogates exonuclease activity and impairs termination of transcription by RNA polymerase II.
Causes mislocalization to the cytoplasm and suppresses the requirement for XRN1 function.
Causes mislocalization to the cytoplasm; when associated with A-534 and A-535.
Causes mislocalization to the cytoplasm; when associated with A-534 and A-537.
Causes mislocalization to the cytoplasm; when associated with A-535 and A-537.
In allele TAP1-1; activates transcription of the promoter-defective yeast SUP4 tRNA(Tyr) allele SUP4A53T61.
Suppresses the requirement for XRN1 function.
Putative uncharacterized protein YLR173W
Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Abolishes both DNA glycosylase and AP lyase activity.
Diminishes both DNA glycosylase and AP lyase activity.
In ERG20-2; 14-fold decrease in FPPS activity.
Translation initiation factor eIF-2B subunit gamma
60S ribosome subunit biogenesis protein NIP7
No cell growth at 23 or 37 degrees Celsius.
No cell growth at 23 or 37 degrees Celsius. Decreases GTP binding and impairs ER-to-Golgi vesicle transport.
No cell growth at 23 or 37 degrees Celsius. Impairs ER-to-Golgi vesicle transport.
No cell growth at 37 degrees Celsius. Decreases GTP binding and ER-to-Golgi vesicle transport.
tRNA threonylcarbamoyladenosine dehydratase 1
6-phosphogluconate dehydrogenase, decarboxylating 2
Uncharacterized membrane protein YML131W
Severely diminishes the enzyme activity.
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit
Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.
Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.
In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.
U3 small nucleolar RNA-associated protein 12
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-21 and A-60.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-149 and A-260.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-149 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-260 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-149; A-260 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-21 and A-83.
Abolishes activity; when associated with A-20; A-21; A-60; A-83; A-135; A-149; A-260 and A-475.
Abolishes activity; when associated with A-20; A-58; A-60; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-60 and A-83.
Abolishes activity; when associated with A-21; A-58; A-60; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-21; A-60 and A-83.
Dehydrodolichyl diphosphate synthase complex subunit NUS1
Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization.
Prevents association with ASH1 and mRNA and leads to its mislocalization.
In npl4-1; nuclear-targeted proteins accumulate in the cytoplasm.
Mitochondrial distribution and morphology protein 31
Impairs SIN3-binding and gene repression activity.
Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation.
Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation.
Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Interacts with target acceptor peptide in protein substrate
Lethal; abolishes interaction with OST1 and WBP1.
Lethal; greatly reduces amount of OST1 in complex.
Lethal; reduces glycosylation; greatly reduces amount of OST1 in complex.
Temperature sensitive and staurosporine sensitive.
Temperature-sensitive; reduces amount of OST1 in complex.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-138, A-141, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-145 and A-282.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-145 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-141, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-16, A-138, A-141, A-145, A-282 and A-292.
Impairs nuclear relocalization in response to arsenic and transcriptional activity, and leads to arsenic hypersensitivity.
Impairs nuclear relocalization in response to arsenic and transcriptional activity, and leads to arsenic hypersensitivity. Loses most of the arsenic-binding capacity; when assciated with A-274.
Retains about 60% of activity; when assocoated with A-92.
Retains about 60% of activity; when assocoated with A-93.
Intron-encoded DNA endonuclease aI5 beta
Ferric reductase transmembrane component 4
Glycolytic genes transcriptional activator GCR1
Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant.
Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production.
Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant.
Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant.
Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant.
tRNA-splicing endonuclease subunit SEN54
Glucose transport transcription regulator RGT1
Probable diacetyl reductase [(R)-acetoin forming] 2
Impairs cytosolic tRNA-cleaving activity; when associated with F-160.
Impairs cytosolic tRNA-cleaving activity; when associated with F-87.
Ribonucleoside-diphosphate reductase small chain 1
Iron sulfur cluster assembly protein 2, mitochondrial
Glycerol-3-phosphate O-acyltransferase 1
In MMM1-6; impairs both mtDNA maintenance and mitochondrial morpholgy.
Maintenance of mitochondrial morphology protein 1
Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial
Reduces the interaction with POL30; when associated with A-42 or D-42.
Leads to a decrease of the catalytic activity and the ubiquitination efficiency.
Prevents the stabilization of the enzyme by oxaloacetate and impairs the catalytic activity.
Putative tyrosine-protein phosphatase OCA6
Uncharacterized protein YDL157C, mitochondrial
Abolishes interaction with the CIA complex. No effect on interaction with YAE1. Strongly decreases iron incorporation into RLI1.
Decreases interaction with YAE1 and the CIA complex. Abolishes iron incorporation into RLI1.
Decreases interaction with YAE1 and the CIA complex. Strongly decreases iron incorporation into RLI1.
Lethal at 37 degrees Celsius; when associated with D-57, D-103 and G-134.
Lethal at 37 degrees Celsius; when associated with G-11, E-83, L-113 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, L-94, S-101 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, E-83 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, E-83 and L-113.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, L-113 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, R-50, L-94 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, R-50, S-101 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, R-50,L-94 and S-101.
Lethal at 37 degrees Celsius; when associated with L-18, D-103 and G-134.
Lethal at 37 degrees Celsius; when associated with L-18, D-57 and D-103.
Lethal at 37 degrees Celsius; when associated with L-18, D-57 and G-134.
Lethal at 37 degrees Celsius; when associated with P-19, E-83, L-113 and G-147. Lethal at 37 degrees Celsius; when associated with R-0, R-50, L-94, S-101 and S-146.
No effect on interaction with YAE1 or the CIA complex. No effect on iron incorporation into RLI1.
Slightly decreases interaction with YAE1 and the CIA complex. No effect on iron incorporation into RLI1.
deca-GX3 motif; required for interaction with YAE1 and the CIA complex
Protein arginine N-methyltransferase SFM1
In GLC5-1; low glycogen accumulation and sporulation deficiency.
Translation initiation factor eIF-2B subunit beta
Abolishes interaction with RSA4. Blocks production of mature 60S subunits, and causes the accumulation of pre-60S particles.
DNA-directed RNA polymerase I subunit RPA12
Necessary and sufficient for recruitment into Pol I
Required for RNA cleavage, but not essential for elongation activity
Probable transcriptional regulatory protein HAH1
In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61
Ribonucleases P/MRP protein subunit POP6
Trafficking protein particle complex II-specific subunit 65
Glucoamylase, intracellular sporulation-specific
GPI ethanolamine phosphate transferase 1
In mcd4-174; defective in endoplasmic reticulum-to-Golgi transport of GPI-anchored proteins.
Aromatic/aminoadipate aminotransferase 1
In cs mutant CDC44-2; causes cell cycle arrest.
In cs mutant CDC44-3/4; causes cell cycle arrest.
In cs mutants CDC44-1/5/8 and CDC44-9; causes cell cycle arrest.
Probable ERAD-associated E3 ubiquitin-protein ligase ASI1
In IQG1-1; causes a defect in cytokinesis at 37 degrees Celsius.
At 37 degrees, growth slows after 6 to 8 hours and cell division stops after 20 hours.
Reduced growth rate at all temperatures; when associated with A-333.
Reduced growth rate at all temperatures; when associated with R-385.
Acetolactate synthase catalytic subunit, mitochondrial
Abolishes mitophagy and impairs interaction with ATG11.
Decreases mitophagy and impairs interaction with ATG11.
Serine/threonine-protein kinase PTK1/STK1
Lethal; Enzyme activity 10% of wild-type.
Non-viable; reduced enzyme activity to 8% of wild-type.
Still viable; activity near to wild-type.
Still viable; little change in enzyme activity.
Still viable; slow growth; near to wild-type enzyme activity.
Translation initiation factor eIF-2B subunit delta
Impairs interaction with MDN1. Blocks progression of the nascent pre-60S subunit and subsequent export to the cytoplasm.
Abolishes interaction with SSC1, and leads to self-aggregation of mtHSP70 and accumulation of uncleaved precursor proteins.
In TIM15-2RH; Abolishes interaction with SSC1, and leads to self-aggregation of mtHSP70 and accumulation of uncleaved precursor proteins.
Mitochondrial protein import protein ZIM17
Temperature sensitive; only partly prevents self-aggregation of mtHSP70 and leads to accumulation of uncleaved precursor proteins at high temperatures.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in URM1)
Interchain (with C-31 in TRX2); transient
No effect on tert-butyl hydroperoxide consumption.
DNA-directed RNA polymerase II subunit RPB2
In gpi10-1; accumulate M2, an abnormal GPI-anchor intermediate due to the absence of third mannose.
ATP-dependent DNA helicase HMI1, mitochondrial
Putative vacuolar protein sorting-associated protein TDA6
Abolishes ATPase and DNA helicase activities but not ATP-binding capacity.
General transcription and DNA repair factor IIH helicase subunit XPD
Target of rapamycin complex 2 subunit TSC11
In RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.
Impairs ORC1-binding and leads to defective association with chromatin.
Interaction with CDC4; required for degradation
Defective in ATP hydrolysis. Missorting of vacuolar proteins.
Inhibits membrane protein sorting to the vacuole.
No ATP hydrolysis. Missorting of vacuolar proteins.
Vacuolar protein sorting-associated protein 4
Does not affect the DNA-binding affinity.
In SGC1-1; suppresses transcriptional defect caused by a GCR1 null mutation.
In SGC1-2/3/4; suppresses transcriptional defect caused by a GCR1 null mutation.
Leads to unstable binding between TYE7 and DNA.
tRNA-specific adenosine deaminase subunit TAD2
Mitochondrial nicotinamide adenine dinucleotide transporter 1
Mediator of RNA polymerase II transcription subunit 12
Reduces 18S rRNA acetylation by 80% and tRNA acetylation by 47%.
Total loss of 18S rRNA acetylation and tRNA acetylation.
Uncharacterized membrane protein YLR326W
Slightly reduced interaction with KTI11.
Loss of catalytic activity and loss of binding to MAS2.
Loss of catalytic activity. No effect on the binding to MAS2.
Loss of zinc binding. Loss of catalytic activity.
Mitochondrial-processing peptidase subunit beta
Abolishes sumoylation in vitro; when associated with R-426.
Abolishes sumoylation in vitro; when associated with R-437.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
D-arabinose dehydrogenase [NAD(P)+] heavy chain
Translation machinery-associated protein 16
Prevents relocalization to the vacuolar membrane.
Sporulation-specific wall maturation protein
Putative uncharacterized protein YOL014W
Resistance to glucose repression protein 1
Decreases localization to nuclear envelope-vacuole contact sites.
Decreases localization to nuclear envelope-vacuole contact sites. Does not appear to affect sorting at early and late endosomes.
Decreases phosphatidylinositol 3-phosphate binding. Vacuolar protein missorting. Abnormal actin cytoskeleton organization. Sensitive to the arginine analog canavanine.
Increases localization to nuclear envelope-vacuole contact sites. Synthetic lethal with MMM1. Normal vacuolar protein sorting and normal sporulation.
Increases localization to peroxisomes. Decreases localization to nuclear envelope-vacuole contact sites and endosomes. Does not appear to affect sorting at early and late endosomes.
Intermembrane lipid transfer protein VPS13
Involved in ARF1 binding and phospholipid binding, including phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3-phosphate; possibly in conjunction with the SHR-BD domain
Normal vacuolar protein sorting and normal sporulation.
Synthetic lethal with MMM1. Normal vacuolar protein sorting and normal sporulation.
Vacuolar protein missorting. Synthetic lethal with MMM1. Normal sporulation.
Impairs degradation of UBC7-substrates, but does not prevent protein degradation.
Prevents polyubiquitin chain attachment and protein degradation.
Thiosulfate sulfurtransferase RDL2, mitochondrial
Completely abolishes the phospholipase activity; when associated with A-88 and A-90.
Completely abolishes the phospholipase activity; when associated with A-90 and A-92.
Partially abolishes the phospholipase activity towards PS and PA. Completely abolishes the phospholipase activity; when associated with A-88 and A-92.
Nucleolar pre-ribosomal-associated protein 1
U6 snRNA-associated Sm-like protein LSm8
Target of rapamycin complex 2 subunit AVO2
Succinate/fumarate mitochondrial transporter
Eliminates glycogen accumulation; when associated with F-230.
Eliminates glycogen accumulation; when associated with F-598.
Mannose 6-phosphate receptor-like protein 1
Target of rapamycin complex 1 subunit KOG1
Altered inheritance of mitochondria protein 3
Impairs dsDNA and ssDNA binding of the MEC1-LCD1 complex.
Conserved oligomeric Golgi complex subunit 6
Mannan polymerase I complex VAN1 subunit
Chromatin structure-remodeling complex subunit RSC4
Dolichyl-phosphate-mannose--protein mannosyltransferase 6
Mitochondrial nicotinamide adenine dinucleotide transporter 2
U3 small nucleolar ribonucleoprotein protein IMP4
Abolished ability to mediate peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal; when associated with H-106.
Abolished ability to mediate peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal; when associated with R-61.
Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins; when associated with A-344.
Does not prevent peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal, such as 3-oxoacyl-CoA thiolase.
Does not prevent peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal, such as 3-oxoacyl-CoA thiolase. Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins; when associated with A-364.
Eukaryotic translation initiation factor 3 subunit C
Target of rapamycin complex 2 subunit AVO1
Moderately reduces the interaction with ATG8.
Stronlgly reduces the interaction with ATG8. Impairs interaction with ATG8; when associated with A-371, A-375, and A-384.
Stronlgly reduces the interaction with ATG8. Impairs interaction with ATG8; when associated with A-371, A-375, and A-387.
Stronlgly reduces the interaction with ATG8. Impairs interaction with ATG8; when associated with A-371, A-384 and A-387.
Stronlgly reduces the interaction with ATG8. Impairs interaction with ATG8; when associated with A-375, A-384 and A-387.
Decreases the association to PtdIns(3)P containing membranes.
Strongly reduces the ubiquitin-binding activity.
Vacuolar protein sorting-associated protein 27
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Increased recombination centers protein 15
Translation termination inhibitor protein ITT1
Required for the localization to the prospore membrane
Dolichyl-phosphate-mannose--protein mannosyltransferase 4
In TEL1-11; short telomere phenotype and impairs DNA-damage checkpoint function at 37 degrees Celsius.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with D-2611-2612-A and E-2631.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with D-2611-2612-A and K-2616.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with K-2616 and E-2631.
Structure-specific endonuclease subunit SLX1
ERAD-associated E3 ubiquitin-protein ligase DOA10
In TRA1-2; when associated with L-604; R-2733; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; P-3145 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; P-3145 and S-3222. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; R-2733; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
Uncharacterized membrane protein YDR090C
Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation.
Diminishes the formation of oligomers, dampens activation of the HOG1 kinase, and impairs growth in high-salt or sorbitol conditions.
60S ribosomal subunit assembly/export protein LOC1
Ran-specific GTPase-activating protein 1
Exists mainly as monomer. Has no peroxiredoxin activity.
Exists mainly as monomer. Has no peroxiredoxin activity. Fails to protect glutamine synthetase against damage by DTT oxidation.
Important for catalytic activity, helps activating the peroxidatic cysteine through H-bonding
Interchain (with C-84 in SRX1); transient
Rab proteins geranylgeranyltransferase component A
Decreases substrate-binding and reduces mannosyltransferase activity.
Dolichyl-phosphate-mannose--protein mannosyltransferase 1
Moderately decreases complex formation with PMT2.
Uncharacterized plasma membrane protein YNL194C
Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing]
U1 small nuclear ribonucleoprotein component SNU71
In strain: CLIB 410, CLIB 413 and Sigma 1278B.
Does not rescue the lethal phenotype of the deletion mutant.
Partially rescues the lethal phenotype of the deletion mutant.
Rescues the lethal phenotype of the deletion mutant.
Topoisomerase I damage affected protein 2
Glucosamine 6-phosphate N-acetyltransferase
Attenuation of the response to Ras proteins.
Abolishes catalytic activity. Reduces the LCA palmitic acid transport activity by 96%. Abolishes the LCB dihydrosphingosine (DHS) transport activity.
Likely essential for ATP utilization. Reduces catalytic activity by 89%. Reduces the LCA palmitic acid transport activity by 71%. has no effect on the LCB dihydrosphingosine (DHS) transport activity.
Leads to reduced colony size when FKH2 is deleted.
Mitochondrial import inner membrane translocase subunit TIM12
Y' element ATP-dependent helicase YLL067C
Uncharacterized membrane protein YCR061W
Phosphopantothenate--cysteine ligase CAB2
Interaction with SRP1; when associated with missing 309-I--E-663.
Mislocalized to the endoplasmic reticulum.
No interaction with SRP1; when associated with missing 309-I--E-663.
No interaction with SRP1; when associated with missing 309-I--E-663. Mislocalized to the endoplasmic reticulum.
Negative cofactor 2 complex subunit beta
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
In PRP28-101; no growth at 15 and 37 degrees Celsius.
In PRP28-102; no growth at 25 degrees Celsius or lower.
In PRP28-103; no growth at 15 and 37 degrees Celsius.
In PRP28-117; no growth at 15 degrees Celsius.
In PRP28-1; no growth at 15 degrees Celsius.
In PRP28-32; no growth at 15 degrees Celsius.
In PRP28-36; slow growth at 30 degrees Celsius, and no growth at 15 and 37 degrees Celsius.
In PRP28-37; no growth at 37 degrees Celsius.
In PRP28-52; no growth at 37 degrees Celsius.
In PRP28-55; no growth at 37 degrees Celsius.
In PRP28-56; no growth at 37 degrees Celsius; when associated with F-440 and E-584.
In PRP28-56; no growth at 37 degrees Celsius; when associated with F-440 and S-546.
In PRP28-56; no growth at 37 degrees Celsius; when associated with S-546 and E-584.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-438 and D-486.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-438 and L-468.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-468 and D-486.
In PRP28-66; no growth at 37 degrees Celsius; when associated with A-541; V-549; N-580 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; N-580 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; V-549 and N-580.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; V-549 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; V-549; N-580 and V-586.
In PRP28-99; no growth at 15 and 37 degrees Celsius.
Pre-mRNA-splicing ATP-dependent RNA helicase PRP28
Displays a reduced affinity for cho-lesterol.
Displays a reduced affinity for cholesterol.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-802; A-804; A-812; A-849 and D-892.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-802; A-804; A-812; K-816 and A-849.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-802; A-804; A-812; K-816 and D-892.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-802; A-804; K-816; A-849 and D-892.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-802; A-812; K-816; A-849 and D-892.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-801; A-804; A-812; K-816; A-849 and D-892.
In M7-1: Changes substrate-specificity towards protopanaxadiol to produce ginsenoside Rh2; when associated with A-802; A-804; A-812; K-816; A-849 and D-892.
Increases the activity toward diosgenin and pregnenolone, but does not affect the activity for cholesterol.
Increases the activity toward diosgenin, but does not affect the activity for cholesterol.
Reduces the activities toward acceptor substrates and completely abolishes the activity with protopanaxadiol.
Double-strand break repair protein MRE11
Further reduces interaction with eIF4E/TIF45; when associated with A-4.
Reduces interaction with eIF4E/TIF45. Prevents pseudohyphal growth. Further reduces interaction with eIF4E/TIF45; when associated with A-9.
Putative tyrosine-protein phosphatase OCA1
Uncharacterized membrane protein YOR034C-A
Signal recognition particle subunit SRP54
Ankyrin repeat-containing protein YCR051W
Regulator of Ty1 transposition protein 102
Reverses the aerobic defects of yeast strains lacking superoxide dismutase (SOD). Associated with elevated copper ion accumulation and increased sensitivity to the toxic effects of copper and cadmium. Exhibits no additional sensitivity to manganese.
Inositol auxotrophy. Impaired ER morphology with the apparition of clumps of ER membrane.
No defect in inositol biosynthesis. Aberrant lipid droplet (LD) budding.
Temperature sensitive. Inositol auxotroph at 37 degrees Celsius, but partially supports growth on inositol at 25 degrees Celsius. Impaired ER morphology and aberrant lipid droplet (LD) budding.
25S rRNA (adenine(2142)-N(1))-methyltransferase
In RHO1-10; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with G-69.
In RHO1-10; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with P-165.
In RHO1-11; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with E-166.
In RHO1-11; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with K-101.
In RHO1-2; temperature sensitive, fails to activate PKC1.
In RHO1-3; temperature sensitive, severely decreases beta-1,3-glucan synthase activation.
In RHO1-4; temperature sensitive, severely decreases beta-1,3-glucan synthase activation.
In RHO1-5; temperature sensitive, fails to activate PCK1.
Locks RHO1 in the GTP-bound form by abolishing GTP hydrolysis.
4 X 5 AA repeats of [DE]-[DE]-[DE]-K-K; highly charged region
Leads to growth defect at 37 degrees Celsius, probably by disrupting the intramolecular interaction of the N-termini with the middle domains; when associated with A-453.
Leads to growth defect at 37 degrees Celsius, probably by disrupting the intramolecular interaction of the N-termini with the middle domains; when associated with A-493.
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Lowers pKa of C-terminal Cys of first active site
Lowers pKa of C-terminal Cys of second active site
U3 small nucleolar RNA-associated protein 14
Loss of function; when associated with S-56.
Loss of function; when associated with S-59.
Uncharacterized membrane protein YLR046C
Uncharacterized membrane protein YFL051C
Probable S-adenosylmethionine-dependent methyltransferase CRG1
ADP-ribosylation factor GTPase-activating protein effector protein 1
C-1-tetrahydrofolate synthase, mitochondrial
Putative uncharacterized oxidoreductase YGL039W
Translation machinery-associated protein 64
No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain.
Regulator of rDNA transcription protein 5
Dolichyl-phosphate beta-glucosyltransferase
DNA-directed RNA polymerase III subunit RPC7
Outward-rectifier potassium channel TOK1
Non-classical export protein 2 homolog 1
Almost completely abolishes catalytic activity.
Completely abolishes catalytic activity; when associated with V-387.
Completely abolishes catalytic activity; when associated with V-389.
Critical for specifying symmetric addition of methyl groups
Protein arginine N-methyltransferase HSL7
Does not completely restore mitochondrial DNA-dependent respiratory chain activities in respiration-defective mutant cells.
Mitochondrial translation optimization protein 1
Reduced ATP-dependent RNA helicase activity.
Strongly reduced unwinding of U4/U6 snRNA.
Recessive mutant; phenotypically null. Eliminates the ability for self-association.
Abrogates kinase activity and transcriptional repression.
Meiotic mRNA stability protein kinase SSN3
Membrane-anchored lipid-binding protein LAM5
Induces a strong reduction in ability to stimulate mtHSP70 activity but does not affect import of proteins into mitochondrial matrix.
Mitochondrial import inner membrane translocase subunit TIM14
Temperature-sensitive mutant that impairs heterodimerization with PAM16 and import of proteins into mitochondrial matrix when incubated at 37 degrees Celsius.
Protein phosphatase 2C homolog 7, mitochondrial
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4
Decreases ATP hydrolysis. Decreases transporter activity.
Iron-sulfur clusters transporter ATM1, mitochondrial
Loss of function; significant decrease in ATP-binding; no homodimerization.
Loss of function; slight decrease in ATP-binding.
Elongated telomeres and severe growth defects.
Altered inheritance of mitochondria protein 11
Endoplasmic reticulum transmembrane helix translocase
Endoplasmic reticulum transmembrane protein 3
Impairs interaction with ARL1 and Golgi localization.
N-linked (GlcNAc...) asparagine; partial
Converts to human ortholog residue: No effect.
Cytochrome c oxidase assembly protein COX15
Fails to rescue the heme a biosynthetic defect of a cox15delta mutant.
Involved in either heme binding or the catalytic mechanism of the enzyme
Mimicks pathogenic substituion found in human patients: Fails to rescue the heme a biosynthetic defect of a cox15delta mutant.
Leads to increased oxidoreductase activity.
Mediator of RNA polymerase II transcription subunit 1
Cytochrome b-c1 complex subunit 9, mitochondrial
Impairs cell growth and lowers aconitase activity.
Impairs cell growth, lowers aconitase activity and strongly decreases interaction with ISU1.
Impairs cell growth, lowers activity of mitochondrial iron-sulfur cluster-containing enzymes, no effect on iron binding and oligomerization.
Impairs oligomerization and iron mineralization.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-103.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-97.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-97 and A-103.
Lowers aconitase activity and no effexct on interaction with ISU1.
Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-79.
Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-82.
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
In Dpl1CKSN_LLLL; impairs the formation of higher oder oligomeric complexes and consequently reduces catalytic activity.
Leads to low activity and reduced substrate affinity.
Putative aldehyde dehydrogenase-like protein YHR039C
Ferric/cupric reductase transmembrane component 1
Probable proline--tRNA ligase, mitochondrial
Methylthioribulose-1-phosphate dehydratase
Unfolded protein response-inducible protein 1
Cytochrome c oxidase subunit 7, mitochondrial
ARF guanine-nucleotide exchange factor 1
Interaction with RRP46 abolished; when associated with F-162.
Interaction with RRP46 abolished; when associated with T-246.
Interaction with RRP46 abolished; when associated with T-274 and Y-276.
Interaction with RRP46 abolished; when associated with Y-230 and T-274.
Interaction with RRP46 abolished; when associated with Y-230 and Y-276.
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13, YIIc12 and YIIc17.
Signal recognition particle subunit SRP21
tRNA pseudouridine(32) synthase, mitochondrial
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3
Hydroxyacylglutathione hydrolase, mitochondrial
Mitochondrial inner membrane protein SHH4
Mitochondrial respiratory chain complexes assembly protein AFG3
Structural maintenance of chromosomes protein 1
ADA histone acetyltransferase complex component 2
Serine/threonine-protein phosphatase 4 catalytic subunit
Abolishes ability to control levels of proteins with 'non-stop'.
Reduced binding to stalled 60S ribosomal subunits.
Putative uncharacterized transporter YOL163W
Decreases ATG8-PE comjugation and autophagy.
Instead of the formation of an intermediate complex with a thiol ester bond between ATG3 (E2-like enzyme) and ATG8 (substrate), a stable complex with an O-ester bond is formed.
Loss of interaction with ATG8 and defect in autophagy and Cvt pathway.
Glutathione peroxidase-like peroxiredoxin 2
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21
Ubiquitin carboxyl-terminal hydrolase 10
Glycerol-3-phosphate dehydrogenase, mitochondrial
In TEF2-1; strongly reduces translation fidelity by increasing the frequency of frameshifting and amino acid misincorporation.
In TEF2-2; strongly reduces translation fidelity by increasing the frequency of frameshifting and amino acid misincorporation.
Increases KM for GTP to 10.3 mM and reduces translation fidelity; when associated with T-152.
Increases KM for GTP to 13.1 mM and reduces translation fidelity. Confers hyperresistance to canavanine.
Increases KM for GTP to 4.2 mM. Preferres XTP over GTP as substrate.
Increases KM for GTP to 6.0 mM and reduces translation fidelity. Increases Km for GTP to 10.3 mM and reduces translation fidelity; when associated with E-156.
N6,N6-dimethyllysine; by EFM4; alternate
N6,N6-dimethyllysine; by EFM7; alternate
Reduced viability upon starvation protein 167
Uncharacterized TLC domain-containing protein YPR114W
Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase YLR345W
Regulator of Ty1 transposition protein 107
Mitochondrial genome maintenance protein MGM101
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-181.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-185.
Defective in ESCRT-I cargo sorting; when associated with D-102.
Defective in ESCRT-I cargo sorting; when associated with D-88.
Defective in ESCRT-I cargo sorting; when associated with D-92.
Defective in ESCRT-I cargo sorting; when associated with D-95.
Defective in ESCRT-I cargo sorting; when associated with D-99.
Reduces ability to activate transcription and to induce invasive growth.
NAD-dependent malic enzyme, mitochondrial
Inhibits splicing; when associated with S-284; N-362 and S-738 in X10SSSVDE.
Inhibits splicing; when associated with S-284; S-737 and S-738 in X10SSSVDE.
Reduces splicing reaction speed. Inhibits splicing; when associated with N-362; S-737 and S-738 in X10SSSVDE.
Reduces splicing reaction speed. Inhibits splicing; when associated with S-284; N-362 and S-737 in X10SSSVDE.
V-type proton ATPase catalytic subunit A, 1st part
V-type proton ATPase catalytic subunit A, 2nd part
Disrupts iron-sulfur (Fe-S) cluster assembly and decreases cytosolic tRNA thiolation. Exhibits constitutive up-regulation of the genes of the cellular iron uptake system.
In POL5dn; has no DNA synthesis activity, but complements the lethality of a pol5 null mutant; when associated with N-623.
In POL5dn; has no DNA synthesis activity, but complements the lethality of a pol5 null mutant; when associated with N-625.
Translation initiation factor IF-2, mitochondrial
Reduces enzyme activity by 35%. Partially impairs dimer formation.
Severely impairs t(6)A37 formation. No effect on dimer formation.
Severely impairs t(6)A37 formation. Partially impairs dimer formation.
Severely impairs t(6)A37 formation. Prevents dimerization.
tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Abolishes phosphatase activity and impairs cardiolipin biosynthesis.
Phosphatidylglycerophosphatase GEP4, mitochondrial
DNA-directed RNA polymerase III subunit RPC3
Suppressor of HU sensitivity involved in recombination protein 1
Putative uncharacterized protein YCL068C
Mitochondrial-processing peptidase subunit alpha
Nascent polypeptide-associated complex subunit beta-2
Ferric reductase transmembrane component 6
U3 small nucleolar RNA-associated protein 18
Vacuolar protein sorting-associated protein 1
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase
26S proteasome regulatory subunit 4 homolog
Hydrolyzes decanoate (C10) esters in contrast to wild-type. Active also on short chain esters from acetate (C2) to hexanoate (C6), but the catalytic efficiency is lower than that of wild-type.
Reduces ATPase activity and interaction with eEF1A. Required for growth at 37 degrees Celsius and causes a 50% reduction of total protein synthesis at permissive temperatures.
Abolishes coenzyme Q biosynthesis. Able to autophosphorylate in cis.
Abolishes coenzyme Q biosynthesis. Enhanced autophosphorylation in cis.
In coq8-2; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-3; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8. Impairs coenzyme Q6 biosynthesis.
In coq8-4; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-5; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8. Impairs coenzyme Q6 biosynthesis.
In coq8-6; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-8; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
Beta-glucosidase-like protein NCA3, mitochondrial
Protein N-terminal and lysine N-methyltransferase EFM7
Does not affect the No-Go Decay (NGD) pathway.
Reduced ability to trigger the No-Go Decay (NGD) pathway, reduced ability to promote degradation of non-functional rRNAs.
Reduced ability to trigger the No-Go Decay (NGD) pathway, reduced ability to promote degradation of non-functional rRNAs; when associated with A-300.
Reduced ability to trigger the No-Go Decay (NGD) pathway, reduced ability to promote degradation of non-functional rRNAs; when associated with A-361.
In SUA5-1; suppresses a translation initiation defect in a CYC1 allele at an aberrant ATG codon.
Glycosylphosphatidylinositol anchor biosynthesis protein 11
Mitochondrial MYO2 receptor-related protein 1
Causes hyperactivity with constitutive induction of PDR12.
Causes hyperactivitywith constitutive induction of PDR12.
Causes hypersensitivity to sorbate through its inability to induce PDR12.
2-deoxy-glucose resistant protein 1, mitochondrial
Putative uncharacterized protein YLR031W
Succinate dehydrogenase assembly factor 2, mitochondrial
Putative cystathionine gamma-synthase YML082W
In rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with L-132.
In rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with P-377.
Phosphatidylethanolamine N-methyltransferase
Restriction of telomere capping protein 5
Trafficking protein particle complex III-specific subunit 85
Uncharacterized mitochondrial outer membrane protein YPR098C
Is rapidly degraded and disrupts stable ATPase assembly.
Probable electron transfer flavoprotein subunit beta
Guanine nucleotide-binding protein subunit beta 1
Important for mediating homomultimerization
Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467.
Inactivates MET30 and prevents MET4 interaction; when associated with A-425.
Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425.
Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467.
Necessary to mediate nuclear localization
Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D.
Putative aryl-alcohol dehydrogenase AAD3
Delays the appearance and decreases the level of 25S rRNA.
Confers interaction with methylated histone H3 'K-4'.
Disrupts interaction with methylated histone H3 'K-4'; abrogates histone acetylation activity of SLIK.
No effect on interaction with methylated histone H3 'K-4'.
No interaction with methylated histone H3 'K-4'.
Increases the specific activity 2-fold and decreases sensitivity to CTP product inhibition 5-fold.
No effect on activity and product inhibition by CTP.
Abnormal TORC1 activation in glutamine-replete conditions. Sensitive to caffeine. Resistance to tunicamycin (endoplasmic reticulum stressor) administered together with FK506 (calcineurin inhibitor).
Abolishes interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Decreases interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Decreases interaction with and activation of TOR1.
Decreases localization to the vacuolar membrane and results in rapamycin sensitivity; when associated with A-475.
Decreases localization to the vacuolar membrane and results in rapamycin sensitivity; when associated with S-522.
Increases activity in presence of glutamine.
May play a role in attenuating TORC1 signaling
Mildly decreases interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Vacuolar import and degradation protein 27
Glycerol-3-phosphate O-acyltransferase 2
In GAT1-TTA1; abolishes both GAT and DHAPAT activities.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-664 and A-668.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-664 and A-671.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-668 and A-671.
tRNA-dihydrouridine(20) synthase [NAD(P)+]
Shows a 4-fold reduced affinity for DNA.
Shows a strongly reduced affinity for linear and circular DNA.
Phosphatidylinositol 4-phosphate 5-kinase MSS4
Restriction of telomere capping protein 1
Inner membrane assembly complex subunit 17
Mitochondrial import inner membrane translocase subunit TIM22
Uncharacterized vacuolar membrane protein YNR061C
Osmosensing histidine protein kinase SLN1
17 X 8 AA tandem repeats of K-K-K-K-E-E-E-E
Interaction with the HAP2-HAP3-HAP5 heteromer
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the mature 60S; when associated with E-95.
Oxidant-induced cell-cycle arrest protein 5
DNA-directed RNA polymerase III subunit RPC5
Terminates elongation following the production of C22 instead of C-26; when associated with K-262.
Terminates elongation following the production of C22 instead of C-26; when associated with L-266.
Strongly reduces HAT activity at pH 9.2. Nearly abolishes HAT activity at pH 8.0, but is not lethal (in vivo). Lethal; when associated with S-334.
Strongly reduces HAT activity, but is not lethal (in vivo). Lethal, when associated with Q-338.
Methionyl-tRNA formyltransferase, mitochondrial
Loss of interaction with SH3 domain of PEX13. No effect on membrane association.
ATP-dependent 6-phosphofructokinase subunit alpha
Abolishes sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'D-718' in subunit beta.
Increases the KM for fructose 6-phosphate 20 fold.
Reduces maximal activity of the holoenzyme by 50%. Completely abolishes catalytic activity; when associated with 'S-348' in subunit beta.
Reduces sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'S-853' in subunit beta.
No effect on MCM2-7 complex helicase activity. Loss of MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM2 A-549.
Impairs the peroxisomal localitation and leads to both nuclear and cytosolic localization.
In strain: Awamori-1, AKU-4011, K1, K5, NRIC 23, NRIC 1413 and NRIC 1685.
Probable phospholipase YOR022C, mitochondrial
Mitochondrial holo-[acyl-carrier-protein] synthase
Probable 26S proteasome regulatory subunit p27
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR1
Abolishes disulfide bond, leading to destabilization of the TIM23 complex.
Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-4.
Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-8.
Mitochondrial import inner membrane translocase subunit TIM17
Impairs interaction with UBC2, but does not affect degradation of N-end rule substrates.
No effect on interaction with UBC2, but inhibits degradation of N-end rule substrates.
U3 small nucleolar ribonucleoprotein protein LCP5
Glyceraldehyde-3-phosphate dehydrogenase 3
Phosphate system positive regulatory protein PHO81
Abolishes interaction with BBC1 and VRP1.
Bypasses the requirement of SHE4 for proper actin cytoskeleton polarization.
In MYO5-1; temperature sensitive loss of function.
Leads to a depolarized actin cytoskeleton and a strong defect in the capacity to internalize STE2.
No growth defect, but leads to a partially depolarized actin cytoskeleton. Accelerates the constitutive internalization of STE2.
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Does not affect the affinity for dUTP but greatly reduces activity.
Exhibits reduced activity and lower substrate affinity.
3-oxoacyl-[acyl-carrier-protein] reductase
Does not inhibit interaction with TTI1 or TTI2. Inhibits interaction with TTI1 or TTI2; when associated with E-345.
Does not inhibit weakly interaction with TTI1 or TTI2. Inhibits interaction with TTI1 or TTI2; when associated with Q-333.
Pheromone-processing carboxypeptidase KEX1
In YME2-4; prevents mtDNA escape, growth on nonfermentable carbon sources and growth at 37 degrees Celsius on glucose.
Signal recognition particle subunit SRP68
Altered inheritance of mitochondria protein 36, mitochondrial
No defect in the interaction with AFG2 or in promoting AFG2 activation.
Reduces interaction with AFG2. Loss of AFG2 activation.
Prevents nuclear localization; when associated with A-82 and A-83.
Prevents nuclear localization; when associated with A-82 and A-85.
Prevents nuclear localization; when associated with A-83 and A-85.
Abolishes SDH activity and complex assembly.
Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-260.
Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-261.
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Vacuolar protein sorting-associated protein 52
Impairs interaction with ATG8 and decreases subsequent reticulophagy; when associated with A-245.
U3 small nucleolar RNA-associated protein 4
Peptide chain release factor 1, mitochondrial
Ubiquinone biosynthesis O-methyltransferase, mitochondrial
Glyceraldehyde-3-phosphate dehydrogenase 1
Eukaryotic translation initiation factor 3 subunit B
Impairs interaction with HCR1 and TIF32, impairs interaction of the eIF-3 complex with eIF-1, eIF-2 and the 40S ribosome, and impairs initiation of translation.
Sufficient for interaction with HCR1 and TIF32
Ubiquitin carboxyl-terminal hydrolase 11
Multifunctional methyltransferase subunit TRM112
Sorting assembly machinery 35 kDa subunit
Decreases interaction with GCN2 and YIH1 but not with GCN20 and ribosomes.
Decreases interaction with GCN20 and polysomal association.
Does not affect ability to activate GCN2.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation; when associated with 1461-A--A-1465.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation; when associated with A-1458.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 782-A-A-783 and 786-A-A-787.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 782-A-A-783 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-765; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-762; A-765; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not interact with GCN2, impairs eIF-2-alpha phosphorylation and fails to derepress GCN4 translation in amino acid-starved cells.
Chromatin modification-related protein YNG2
Eukaryotic translation initiation factor 5B
Impairs the GTPase activity, but not the ribosome joining function.
Reduces GTP binding and impairs subunit joining and ribosome-dependent GTP hydrolysis.
Altered inheritance of mitochondria protein 24, mitochondrial
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
Abolishes interaction with VSP4 and dimerization.
Abolishes interaction with VSP4, no effect on dimerization.
Abolishes interaction with VSP60 and DID2.
Vacuolar protein sorting-associated protein VTA1
U1 small nuclear ribonucleoprotein component PRP42
Probable aryl-alcohol dehydrogenase AAD4
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial
Coenzyme Q-binding protein COQ10, mitochondrial
Increased recombination centers protein 19
tRNA modification GTPase MSS1, mitochondrial
Alpha-ketoglutarate dehydrogenase subunit 4, mitochondrial
Sorting assembly machinery 50 kDa subunit
Peroxisomal hydratase-dehydrogenase-epimerase
Transcription initiation factor IIF subunit beta
Abolishes ubiquitin binding. Defective activation of the ribosome quality control (RQC) pathway.
Abolishes cell surface localization. Abolishes acid trehalase activity.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-2 and R-27.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-2 and R-37.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-27 and R-37.
Protein mislocalized to the vacuolar membrane in a small proportion of cells.
Decreased S-adenosyl-L-methionine-binding.
Decreased aminocarboxypropyltransferase activity; when associated with A-60 and A-131.
Decreased aminocarboxypropyltransferase activity; when associated with A-60 and A-65.
Decreased aminocarboxypropyltransferase activity; when associated with A-65 and A-131.
Does not affect S-adenosyl-L-methionine-binding.
Reduced aminocarboxypropyltransferase activity.
Glutathione-independent glyoxalase HSP31
Eliminates arginine-specific stalling of ribosomes at the termination codon.
Confers sensitivity to HU indicating a disturbed activity in DNA replication.
Confers sensitivity to HU indicating a disturbed activity in DNA replication; confers a SPT- phenotype indicating a disturbed activity in transcription.
In pob3-11; causes severe defects in rate of growth; when associated with C-109.
In pob3-11; causes severe defects in rate of growth; when associated with H-20.
In pob3-1; causes severe defects in rate of growth; when associated with K-419 and T-489.
In pob3-1; causes severe defects in rate of growth; when associated with R-78 and K-419.
In pob3-1; causes severe defects in rate of growth; when associated with R-78 and T-489.
In pob3-21; causes severe defects in rate of growth.
No change of sensitivity to HU; confers a SPT- phenotype indicating a disturbed activity in transcription.
NADPH-dependent 1-acyldihydroxyacetone phosphate reductase
Nucleolar pre-ribosomal-associated protein 2
Mitochondrial morphogenesis protein SLD7
Impairs autophosphorylation and mating efficiency.
Impairs interaction with BEM1; when associated with A-477.
Impairs interaction with BEM1; when associated with G-475.
Impairs phosphorylation of STE11 and histone H2B and mating efficiency.
Abolishes binding to COPI coatomer complex.
Multicopy suppressor of SEC21 protein 27
Meiotic sister-chromatid recombination protein 6, mitochondrial
Cytochrome c oxidase subunit 12, mitochondrial
Not viable in aerobic conditions. Lethal; when associated with S-61.
Abolishes interaction with FIP1; when associated with R-485.
Abolishes interaction with FIP1; when associated with Y-489.
Reduces rate of adenylyltransfer about four-fold.
Reduces rate of adenylyltransfer by half.
Slightly reduced rate of adenylyltransfer.
ADP-ribosylation factor-binding protein GGA1
Vacuolar protein sorting-associated protein 68
Structural maintenance of chromosomes protein 4
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831 and A-842.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831, A-842 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-804, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-802, A-804, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-657, A-802, A-804, A-831, A-842, A-948 and A-969.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-113 and R-121.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-113 and R-138.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-121 and R-138.
Abolishes interaction with ATG11 and disrupts Cvt, but not bulk autophagy.
Does not affect ubiquitination by the SCF(MET30) complex and subsequent degradation.
Impaired selective autophagy. Does not affect ubiquitination by the SCF(MET30) complex.
In Acidic mutant; abolished autophagy caused by defects in phospholipid scramblase activity.
In Basic mutant; impaired autophagy; when associated with A-560 and A-576.
In Basic mutant; impaired autophagy; when associated with A-560 and A-723.
In Basic mutant; impaired autophagy; when associated with A-576 and A-723.
In Entrance 1 mutant; does not affect autophagy; when associated with A-385.
In Entrance 1 mutant; does not affect autophagy; when associated with S-545.
In Entrance 2 mutant; does not affect autophagy.
In Entrance 3 mutant; impaired autophagy possibly caused by mislocalization; when associated with 497-A--A-501.
In Entrance 3 mutant; impaired autophagy possibly caused by mislocalization; when associated with A-392.
In Hexamer mutant; does not affect autophagy.
In Junction mutant; does not affect autophagy.
In Wall mutant; abolished autophagy caused by defects in phospholipid scramblase activity.
Phospho-mimetic mutant; increased selective autophagy. Prevents ubiquitination by the SCF(MET30) complex.
Conserved oligomeric Golgi complex subunit 3
Citrinin resistance protein, mitochondrial
Coupling of ubiquitin conjugation to ER degradation protein 1
Histone acetyltransferase type B subunit 2
Interaction with the histone H4 N-terminus
In afg2-18, drg1-18 or drg1-ts; temperature sensitive mutant. At the restrictive temperature of 37 degree Celsius, impaired growth. Severe loss of ATPase activity, homohexamer formation and 60S ribosomal subunit maturation. Prevents the release of shuttling proteins NOG1, RLP24 and ARX1 from cytoplasmic pre-60S particles resulting in their cytoplasmic accumulation. Loss of interaction with RLP24 and pre-60S particles. Restores growth and formation of 60S ribosomal subunit maturation but not catalytic activity or oligomerization; when associated with L-343.
In dgr1-sup*; moderate loss of catalytic activity. No growth defect. Restores growth and formation of 60S ribosomal subunit maturation but not catalytic activity or oligomerization; when associated with S-457.
In drg1-11; severe loss of ATPase activity. Severe loss of oligomerization. Resistant to diazaborine-mediated growth inhibition.
In drg1-1; slight loss of ATPase activity. No effect on affinity for ATP or oligomerization. Resistant to diazaborine-mediated growth inhibition. No defect in RLP24 release from pre-60S ribosomal particles in the absence or in the presence of diazaborine.
In drg1-3; resistant to diazaborine-mediated growth inhibition.
In drg1-4; resistant to diazaborine-mediated growth inhibition.
Increases ATPase activity and reduces affinity for ATP. Mild defect in oligomerization.
Increases ATPase activity. Loss of oligomerization.
Increases basal ATPase activity. Reduces RLP24-mediated activation. Does not affect interaction with RLP24. Prevents the release of shuttling proteins RLP24, NOG1 and MEX67 from cytoplasmic pre-60S ribosomal particles. Does not affect the interaction with pre-60S ribosomal particles.
Reduces basal and RLP24-dependent ATPase activity. Increases interaction with RLP24. Slightly reduces RLP24 release. Does not affect composition of pre-60S ribosomal particles or growth.
Y' element ATP-dependent helicase YIL177C
In mdm17; loss of function at 37 degrees Celsius.
Loss of function; most MGM1 processed to s-MGM1.
Sphingoid long-chain base transporter RSB1
In RRP4-1; temperature-sensitive(ts) lethal mutation.
Decreases quinone reductase activity. Little effect on complex assembly.
Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial
Histone-lysine N-methyltransferase, H3 lysine-36 specific
Reduces dramatically histone methyltransferase activity toward nucleosomes.
Increased sensitivity toward ionizing radiation.
Average mass with 1 acetylation and 1 methylation modification.
SCF E3 ubiquitin ligase complex F-box protein GRR1
Circumvents the requirement of the CTD to enhance substrate trans-location through the hydrophobic core.
Decreases the affinity for the substrate.
Leads to increased competence to acidify the submembrane pH while losing the signaling capability.
Preserves transport activity while impairing the filamentation capacity.
Silences the autoinhibition of MEP2, conferring transport activity even in the absence of the NPR1 kinase.
Decreases the interaction with CDC123, when associated with A-220.
Decreases the interaction with CDC123, when associated with A-350.
Decreases the interaction with CDC123, when associated with L-223.
Decreases the interaction with CDC123, when associated with S-345.
2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
Abolishes diphthamide modification of elongation factor 2. Resistance to diphtheria toxin and sordarin. Sensitive to hygromycin B.
Normal sensitivity to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and leads to abnormal reduction of the DPH1 [4Fe-4S] cluster; when associated with S-362.
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and leads to abnormal reduction of the DPH1 [4Fe-4S] cluster; when associated with S-107.
Cytosolic iron-sulfur protein assembly protein 1
Impaired in cytosolic Fe/S protein assembly.
tRNA (guanosine(18)-2'-O)-methyltransferase
Cytochrome c oxidase assembly protein COX11, mitochondrial
Conserved oligomeric Golgi complex subunit 5
Required for ERAD-L function and HRD1 oligomer formation
Chromatin structure-remodeling complex protein RSC3
Serine/threonine-protein phosphatase PPQ
Asparagine synthetase [glutamine-hydrolyzing] 2
Impairs heterodimer formation with GPN2 and GPN3.
Impairs nuclear localization of RNAPII, but does not impair RNAPII binding.
Impairs nuclear localization of RNAPII. Completely abolishes RNAPII binding.
3 X 10 AA tandem repeats of Q-Q-G-Y-Y-Q-Q-G-P-P
Cysteine-rich and transmembrane domain-containing protein YDR210W
In strain: SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI11.5/a, SCI11.5/b, SCI11.5/c and SCI11.5/d.
In strain: SCI7.2/a, SCI7.2/d, SCI11.5/b and SCI11.5/d.
In strain: SCI7.2/b, SCI11.5/a and SCI11.5/c.
In strain: SCI7.2/b, SCI7.2/d and SCI11.5/c.
In strain: SCI7.2/b,SCI7.2/d and SCI11.5/c.
Cytochrome B pre-mRNA-processing protein 2
Putative uncharacterized protein YLR157W-D
In DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-661.
In DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-664.
In DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-680.
Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands.
Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands. In DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-674.
Uncharacterized membrane protein YNR062C
In strain: CLIB 219, CLIB 410, CLIB 413, CLIB 630 haplotype Ha1, K1, YIIc12 haplotype Ha2 and CLIB 382.
Uncharacterized membrane protein YNL320W
ERAD-associated E3 ubiquitin-protein ligase component HRD3
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3
In allele CUS1-54; cold-sensitive growth suppressor.
Translation machinery-associated protein 22
Ketol-acid reductoisomerase, mitochondrial
RNA polymerase I-specific transcription initiation factor RRN6
U3 small nucleolar RNA-associated protein 15
In PRP5-1; no growth at 37 degrees Celsius and impairs pre-spliceosome formation in vitro.
Pre-mRNA-processing ATP-dependent RNA helicase PRP5
Association with (pre-)40S ribosomal subunit
Inhibits cell growth; enhances association with pre-40S ribosomal subunits; inhibits 20S pre-rRNA to mature 18S rRNA processing; translation initiation-like defect.
Does not accumulate in the nucleus in response to cadmium.
Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-356.
Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-387.
Temperature- and formamide-sensitive; abolishes cleavage and polyadenylation activity.
Temperature- and formamide-sensitive; impaired polyadenylation activity.
Putative magnesium-dependent phosphatase YER134C
Shifts the coenzyme preference of the enzyme from NAD(+) to NADP(+).
Abolishes the ability to complement a yeast strain carrying a deletion for this gene.
Succinate dehydrogenase assembly factor 1, mitochondrial
Decreases activity. Inositol auxotrophy.
Leads to accumulation of 35S and 27SB pre-rRNAs and a net 40S ribosomal subunit defect.
Not palmitoylated, rapidly degraded in a TUL1-dependent manner.
Results in an aktivated t-SNARE that confers endocytosis, but not exocytosis.
t-SNARE affecting a late Golgi compartment protein 1
Inner membrane assembly complex subunit 22
Can only cleave double-flap structures with a 3' 1-nucleotide tail. Has no exonuclease activity.
Deficient in double and single flap endonuclease cleavage and exonucleolytic cleavage.
Deficient in exonuclease and gap endonuclease activities, but retains almost all of its flap endonuclease activity.
Reduces interaction with POL30 more than 100 fold.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius. Impairs RNA binding in vitro.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-89. Impairs RNA binding in vitro.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-91. Impairs RNA binding in vitro.
Impairs sorting; when associated with D-199.
Impairs sorting; when associated with D-202.
Vacuolar protein-sorting-associated protein 46
Ethionine resistance-conferring protein 1
Ribosome quality control complex subunit 1
Sporulation-specific mitogen-activated protein kinase SMK1
25S rRNA (uridine(2843)-N(3))-methyltransferase
Cell wall integrity and stress response component 3
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
4.5 X approximate tandem repeats, Thr-rich
ADP-ribosylation factor GTPase-activating protein GCS1
In GCS1-1; severely affect the function.
4-hydroxy-4-methyl-2-oxoglutarate aldolase
Decreases interaction with elongator complex subunits ELP2 and ELP5. Resistance to zymocin. Sensitive to thermal stress and caffeine. Normal sensitivity to diphtheria toxin and interaction with ATS1/KTI13.
Little or no effect on interaction with ATS1/KTI13.
Required for interaction with the elongator complex
Resistance to zymocin. Sensitive to thermal stress, diphtheria toxin, and caffeine.
In strain: ATCC 76625 / YPH499, MMW1-15 and MMW1-2.
In strain: ATCC 76625 / YPH499,MMW1-15, MMW1-2 and S288c / YPH1.
In strain: Bb32, M1-2A, M2-8, MMR2-5, MMW1-15h2, MMW1-2h2 and Sgu52F.
Uncharacterized transcriptional regulatory protein TBS1
Almost complete loss of enzyme activity.
Retains more than 40% of the wild-type enzyme activity.
Carboxylic acid transporter protein homolog
In fis1-L80P; no interaction with MDV1; mitochondrial fission is blocked; DNM1 assembly at punctate structures normal as in wild-type.
Choline/ethanolaminephosphotransferase 1
JmjC domain-containing histone demethylation protein 1
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Vacuolar protein sorting-associated protein 20
12000-fold decrease in catalytic activity and 50000-fold decrease in catalytic efficiency, with ferricyanide as electron acceptor.
Abolishes binding to and packaging of cargo protein BET1.
Truncated non-functional calcium-binding mitochondrial carrier SAL1-1
Abolishes phosphorylation; when associated with A-44; A-59; A-103; A-115 and A-406.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-115.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-406.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-115 and A-406.
Abolishes phosphorylation; when associated with A-44; A-95; A-103; A-115 and A-406.
Abolishes phosphorylation; when associated with A-59; A-95; A-103; A-115 and A-406.
Anaphase-promoting complex subunit CDC16
In CDC16-183; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC16-1; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC16-264; G2/M cell cycle arrest at 36 degrees Celsius.
Abolishes helicase activity resulting in elongated telomeres; binds normally to DNA substrates.
In PIF1-m1; loss of mitochondrial function.
Valine/tyrosine/tryptophan amino-acid permease 1
Abolished monoubiquitination by PEX2, promoting polyubiquitination by PEX10 and degradation. Loss of UBE2D2-independent ubiquitination. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with R-18 and R-24.
Loss of UBE2D2-dependent ubiquitination. No effect on its function. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with A-6 and R-18.
Loss of UBE2D2-dependent ubiquitination. No effect on its function. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with A-6 and R-24.
25S rRNA (adenine(645)-N(1))-methyltransferase
Impaired methyltransferase activity and ribosomal 60S subunit biogenesis. Does not affect pre-rRNA cleavage at site A2 activity.
In RRP8-1; no effect. Synthetic lethal with GAR1 mutant allele lacking its N- and C-terminal glycine/arginine-rich (GAR) domains.
Calcium/calmodulin-dependent protein kinase II
Translation machinery-associated protein 10
In strain: CLIB 219, CLIB 410, CLIB 413 and YIIc17 haplotype Ha2.
In strain: YIIc12 and YIIc17 haplotype Ha1.
CCR4-NOT transcriptional complex subunit CAF120
Constitutive nuclear location and transcription activation.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with A-620 and T-629.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with T-598 and T-620.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with T-598 and T-629.
Dominant negative transcription activator.
In diamide-induced nuclear retained form
In diamide-induced nuclear retained form; alternate
In peroxide stress-induced nuclear retained form; alternate
Interchain (with C-36 in HYR1); transient; alternate
Uncharacterized WD repeat-containing protein YNL035C
DNA-directed RNA polymerase II subunit RPB3
Dilute domain-containing protein YPR089W
Antisense of depressing factor protein 1
pH-response regulator protein palH/RIM21
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit
Abolishes interaction with ELP1/IKI3 and ELP3.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-628 and A-654.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-628 and A-675.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-654 and A-675.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-628, A-654 and A-675.
Determinant for substrate specificity and/or activity
Loss of catalytic activity, but not required for pre-rRNA processing.
Required for correct nucleolar localization
12 X 6 AA approximate tandem repeats, Ser/Thr-rich
Mitochondrial intermembrane space cysteine motif-containing protein MIX14
Probable kynurenine--oxoglutarate transaminase BNA3
NEDD8-activating enzyme E1 regulatory subunit
Abolishes binding to N6-methyladenosine (m6A)-containing RNAs.
Pleiotropic ABC efflux transporter of multiple drugs
Abolishes insertion into mitochondrial membranes.
Greatly reduced expression. Mislocalization to mitochondrial matrix.
Slightly reduced expression. Does not affect mitochondrial membrane localization but shows weaker membrane association.
Structural maintenance of chromosomes protein 6
Ceramide very long chain fatty acid hydroxylase SCS7
Confers resistance to Syringomycin E, showing impaired catalytic activity.
Maintains the susceptibility to Syringomycin E, showing no effect on catalytic activity.
Reduces the susceptibility to Syringomycin E, showing reduced catalytic activity.
Ribosome-releasing factor 2, mitochondrial
Nuclear cap-binding protein complex subunit 1
U3 small nucleolar RNA-associated protein 10
Cytoplasmic 60S subunit biogenesis factor REI1
Complete loss of covalent binding to ATG12.
No effect on conjugating activity. Normal autophagic activity.
Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy.
Decreases responsiveness to mannose and fructose.
In SNF3-1; constitutively signaling glucose receptor.
Inosine-5'-monophosphate dehydrogenase 3
Transcriptional activator/repressor MOT3
Abrogates both ligand binding and signaling.
Causes a weak constitutive activation of the mating pathway.
Causes a weak constitutive activation of the mating pathway. Leads to reduced mating ability; when associated with A-2331.
Causes constitutive activation of the mating pathway.
Disrupts the interaction with G-protein complex and reduces cell responsiveness to alpha-factor.
Disrupts the interaction with G-protein complex and reduces cell responsiveness to alpha-factor; when associated with G-234.
Disrupts the interaction with G-protein complex and reduces cell responsiveness to alpha-factor; when associated with S-233.
Dominant negative mutation that inhibits the response to mating pheromone in cells that also express wild-type receptors.
Impairs internalization in response to alpha-factor-binding.
Leads to reduced mating ability; when associated with A-241.
Results in 25-fold increased affinity and a 5-fold-higher basal level of signaling.
Results in 5-fold increased affinity and a weak constitutive activity.
Strongly decreases the binding of alpha-factor; when associated with E-478.
Strongly decreases the binding of alpha-factor; when associated with E-48.
Substantially reduces dimerization and signaling without affecting agonist binding.
ADP-ribosylation factor-binding protein GGA2
Loss of chelatase and dehydrogenase activity.
Loss of dehydrogenase activity. No effect on chelatase activity.
No effect on dehydrogenase or chelatase activity.
Uncharacterized membrane protein YBR220C
In NH1; causes resistance to the antifungal antibiotic pradimicin.
Reduces binding of the 4-aspartylphosphate of SLN1.
Impairs plasma membrane ingression; when associated with A-28.
Impairs plasma membrane ingression; when associated with A-31.
Decreases methylation of cytidine in tRNA(Phe).
Methylation of cytidine in tRNA(Phe) appears normal.
Required for interaction with RTT10/TRM734
Severely decreases methylation of guanosine in tRNA(Phe). Decreases cell population growth.
Severely decreases methylation of guanosine in tRNA(Phe). Methylation of cytidine in tRNA(Phe) appears normal.
tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
Abolishes interaction with SEC62; defect in protein translocation.
Lead to defects in spindle pole body assembly.
Loss of function; when associated with A-1236.
Loss of function; when associated with A-608.
Mediator of RNA polymerase II transcription subunit 13
Uncharacterized RNA-binding protein YGR250C
Plasma membrane-associated coenzyme Q6 reductase PGA3
Cyclin-dependent kinases regulatory subunit
Suppresses postrecruitment-defective SPT15/TBP alleles.
Putative nucleotide-sugar transporter YMD8
Eukaryotic translation initiation factor 2 subunit alpha
Inhibits derepression of GCN4 expression in amino acid, purine, and glucose-starved cells.
Weakly impairs derepression of GCN4 expression in amino acid-starved cells.
Abolishes catalytic activity. Fails to localize to lipid droplet biogenesis sites on the ER.
Phospholipid:diacylglycerol acyltransferase
Putative uncharacterized protein YML083C
23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Chromatin modification-related protein EAF1
Vacuolar transporter chaperone complex subunit 1
Vacuolar protein sorting-associated protein 64
Membrane-anchored lipid-binding protein LAM1
Phosphatidylglycerol/phosphatidylinositol transfer protein
25S rRNA (cytosine(2870)-C(5))-methyltransferase
Cannot complement loss of wild type NOP2.
Fails to ctalyze the C-5 methylation of the C2870 residue and strongly affects 60S biogenesis.
Ferric reductase transmembrane component 3
Intrastrand cross-link recognition protein
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1
Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport.
Mitochondrial zinc maintenance protein 1, mitochondrial
DNA polymerase zeta processivity subunit
Serine/threonine-protein phosphatase 1 regulatory subunit GAC1
Respiratory supercomplex factor 1, mitochondrial
Y' element ATP-dependent helicase YJL225C
U3 small nucleolar ribonucleoprotein protein IMP3
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Putative uncharacterized protein YLR415C, mitochondrial
Dihydrosphingosine 1-phosphate phosphatase LCB3
Impairs dihydrosphingosine 1-phosphate phosphatase activity.
Impairs nuclear export; when associated with A-12.
Impairs nuclear export; when associated with A-15.
In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with A-148.
In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with N-151.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-166 and Q-259.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-166 and Q-362.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-259 and Q-362.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with Q-166; Q-259 and Q-362.
In PAB1-157; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-16; reduces affinity for oligo(A) about 100-fold, impairs poly(A)-dependent translation, but still interacts with eIF4G; when associated with V-170. In PAB1-15; fails to bind RNA; when associated with V-170; V-263 and V-366.
In PAB1-175; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-180; abolishes poly(A)-dependent translation and greatly reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.
In PAB1-184; abolishes poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.
In PAB1-193; moderately reduces stimulation of cap-dependent translation in vitro.
In PAB1-199; moderately reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-6; selectively reduces poly(A) RNA binding. In PAB1-16; reduces affinity for oligo(A) about 100-fold, impairs poly(A)-dependent translation, but still interacts with eIF4G; when associated with V-83. In PAB1-15; fails to bind RNA; when associated with V-83; V-263 and V-366.
In PAB1-7. In PAB1-15; fails to bind RNA; when associated with V-83; V-170 and V-366.
In PAB1-8; selectively reduces poly(U) RNA binding. In PAB1-15; fails to bind RNA; when associated with V-83; V-170 and V-263.
Polyadenylate-binding protein, cytoplasmic and nuclear
Required and sufficient for nuclear export
Required and sufficient for nuclear import
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1
Cytochrome b-c1 complex subunit Rieske, mitochondrial
Uncharacterized ABC transporter ATP-binding protein YDR061W
Trafficking protein particle complex subunit BET5