Moybdenum cofactor oxidoreductase dimerisation
Gamma-glutamylcyclotransferase AIG2-like
Major facilitator superfamily (MFS) profile
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Peptide methionine sulfoxide reductase MsrA
Dinitrogenase iron-molybdenum cofactor biosynthesis
Aldehyde ferredoxin oxidoreductase N-terminal
Tetrapyrrole biosynthesis uroporphyrinogen III synthase
Extracytoplasmic solute receptor protein
Molybdopterin cofactor biosynthesis C (MoaC)
UDP-N-acetylenolpyruvoylglucosamine reductase
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Plays an important role in substrate specificity
TRAP C4-dicarboxylate transport system permease DctM subunit
Penicillin-binding protein transpeptidase
3-hydroxyacyl-CoA dehydrogenase C-terminal
3-hydroxyacyl-CoA dehydrogenase NAD binding
Sigma 54 modulation/S30EA ribosomal protein C-terminal
Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding
Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic
Phosphate import ATP-binding protein PstB
Bacterial type II secretion system protein E
Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal
Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding
Galactose-1-phosphate uridyl transferase C-terminal
Galactose-1-phosphate uridyl transferase N-terminal
Ribose-phosphate pyrophosphokinase N-terminal
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
Cytochrome c-type biogenesis protein CcmF C-terminal
Membrane insertase YidC/Oxa/ALB C-terminal
ABC transporter substrate-binding protein PnrA-like
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Glycosyltransferase subfamily 4-like N-terminal
Holliday junction branch migration complex subunit RuvB
O-(5'-phospho-DNA)-tyrosine intermediate
Alpha-D-phosphohexomutase alpha/beta/alpha
RNA 2-O ribose methyltransferase substrate binding
Acetyl-CoA hydrolase/transferase C-terminal
Acetyl-CoA hydrolase/transferase N-terminal
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
4-hydroxy-tetrahydrodipicolinate synthase
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
RNA binding; important for wobble base 34 recognition
Important for interaction with partner proteins
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
ATP-dependent DNA helicase RecG C-terminal
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
Involved in Mg(2+) ion dislocation from EF-Tu
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
Ribosomal RNA small subunit methyltransferase G
Nitrite/Sulfite reductase ferredoxin-like
May be important for ligand binding specificity and FMN binding
No effect on the orientation of the bound flavin.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
Increases the channeling efficiency of propanaldehyde from 57% to 94%.
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Pyridine nucleotide-disulphide oxidoreductase dimerisation
3-phosphoshikimate 1-carboxyvinyltransferase
4-hydroxyphenylacetate 3-monooxygenase, reductase component
Aspartate/ornithine carbamoyltransferase Asp/Orn-binding
Aspartate/ornithine carbamoyltransferase carbamoyl-P binding
Polysaccharide chain length determinant N-terminal
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
DNA-directed RNA polymerase subunit omega
Transcription antitermination protein NusB
CRISPR-associated endonuclease/helicase Cas3
Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom
Probable 2-phosphosulfolactate phosphatase
Tryptophan synthase beta chain-like PALP
Probable dual-specificity RNA methyltransferase RlmN
10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
Important for prephenate aminotransferase activity
NIF system FeS cluster assembly NifU N-terminal
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
Septum formation inhibitor MinC C-terminal
1,4-alpha-glucan branching enzyme TTHA1902
Almost complete loss of branching activity but 10-fold increase in hydrolytic activity.
Complete loss of enzymatic activity. Binds amylose but not glycogen.
Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Redox-sensing transcriptional repressor Rex
Imidazole glycerol phosphate synthase subunit HisF
Loss of XDG activity, but retains much of its UDG and HDG activities.
Loss of XDG activity. Decrease in UDG and HDG activities.
Loss of XDG activity. Strong decrease in UDG and HDG activities.
Loss of xanthine DNA glycosylase (XDG) activity. Decrease in uracil DNA glycosylase (UDG) and hypoxanthine DNA glycosylase (HDG) activities. Shows low activity for the removal of uracil from U/G or thymine from T/G.
Minimal effect on UDG and XDG activities. Strong reduction on HDG activity on A/I and T/I base pairs.
Strong decrease in UDG, HDG and XDG activities.
Bifunctional glucose-6-phosphate/mannose-6-phosphate isomerase C-terminal
Bifunctional oligoribonuclease and PAP phosphatase NrnA
Decreased substrate affinity (KM 420 uM) and catalytic activity for a ssDNA 6-mer.
P pilus assembly protein, chaperone PapD
Probable nicotinate-nucleotide adenylyltransferase
Essential for discrimination between tRNA(Glu) and tRNA(Gln)
Interaction with tRNA; via carbonyl oxygen
Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
Great decrease in chaperone activity. Retains ATPase activity and oligomerization.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-167.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-350.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-353.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-355.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-464.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-467.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-475.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-520.
Loss of ability to bind ATP. Residual ATPase activity.
No effect on ability to bind ATP. Decrease in ATPase activity.
No effect on ability to bind ATP. Increase in ATPase activity.
Glutamyl-tRNA(Gln) amidotransferase subunit A
Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop
Arg finger, required for nucleotide flipping
Required for phosphate rotation/nucleotide flipping
ATP-dependent Clp protease proteolytic subunit
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Pyridoxal 5'-phosphate synthase subunit PdxT
N(4)-bis(aminopropyl)spermidine synthase C-terminal
Interaction with polymerase core subunit RpoC
Holliday junction branch migration complex subunit RuvA
Only one RuvA tetramer is found in the RuvA-RuvB-HJ complex, cannot form octameric RuvA, poor branch migration, poorly stimulates RuvBATPase.
Only one RuvA tetramer is found in the RuvA-RuvB-HJ complex, cannot form octameric RuvA. Binds HJDNA, poor branch migration, poorly stimulates RuvBATPase.
Pyruvate dehydrogenase E1 component middle
2-oxoisovalerate dehydrogenase subunit beta
Cytochrome c-type biogenesis protein CcmE
Helical; Signal-anchor for type II membrane protein
DNA-directed RNA polymerase subunit alpha
SUF system FeS cluster assembly SufBD N-terminal
3-methyl-2-oxobutanoate hydroxymethyltransferase
Nucleophile; cysteine thiosulfonate intermediate
Phosphoadenosine phosphosulphate reductase
PrcB C-terminal domain-containing protein
Molybdopterin cofactor biosynthesis MoaD-related C-terminal
Large decrease in activity and a small decrease in substrate affinity.
Phosphoribosylformylglycinamidine synthase subunit PurQ
CRISPR-associated protein Cas6 C-terminal
Sodium symporter small subunit predicted
Transcription elongation factor GreA/GreB C-terminal
Transcription elongation factor GreA/GreB N-terminal
Aconitase A/isopropylmalate dehydratase small subunit swivel
Electron transfer flavoprotein alpha/beta-subunit N-terminal
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Adaptive response protein AidB N-terminal
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Gly-cisPro motif, important for rejection of L-amino acids
Calcium/calmodulin-dependent protein kinase II association-domain
Putative membrane protein insertion efficiency factor
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Fibronectin type-III domain-containing protein
Glutamine synthetase and cystathionine beta-lyase binding protein
Resolvase/invertase-type recombinase catalytic
Single-stranded-DNA-specific exonuclease RecJ
Lipid/polyisoprenoid-binding YceI-like domain-containing protein
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
RND efflux pump membrane fusion protein barrel-sandwich
Ribonucleotide reductase large subunit C-terminal
Urease accessory protein UreH-like transmembrane
Transcription regulator AsnC/Lrp ligand binding
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Transposase IS116/IS110/IS902 C-terminal
Flavocytochrome c sulphide dehydrogenase flavin-binding
Heat-inducible transcription repressor HrcA C-terminal
Probably phosphorylated, more strongly associated with the 50S ribosomal subunit.
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
ATP-dependent Clp protease ATP-binding subunit ClpX
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Helix-hairpin-helix DNA-binding motif class 1
Polymerase/histidinol phosphatase N-terminal
Pyruvate phosphate dikinase AMP/ATP-binding
DNA-directed RNA polymerase subunit beta
UPF0173 metal-dependent hydrolase TTHA1283
8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Cell envelope-related transcriptional attenuator
Chromosomal replication initiator protein DnaA
Cytochrome b/b6 C-terminal region profile
Cytochrome b/b6 N-terminal region profile
Aspartate/homoserine dehydrogenase NAD-binding
Glutamyl-tRNA(Gln) amidotransferase subunit C
Carbamoyl-phosphate synthase small chain
Decreases amount and stability of iron-sulfur center 4.
Ribosomal RNA large subunit methyltransferase H
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Has some defects in posttransfer editing activity.
No change in posttransfer editing activity.
Outer membrane lipoprotein carrier protein LolA
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx.
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with A-250.
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with N-226.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+) and Ca(2+).
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-304 and A-307.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-304 and A-383.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-307 and A-383.
Does not affect activity. Increases Ni(2+) sensitivity.
Still possesses a slight channel activity.
Cation-transporting P-type ATPase N-terminal
V-type ATP synthase subunit I N-terminal
Vacuolar ATPase subunit I N-terminal proximal lobe
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
N-[TS] motif that is essential for LysX substrate specificity
Ribosomal RNA small subunit methyltransferase B
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
Pyruvate/ketoisovalerate oxidoreductase catalytic
tRNA (adenine(58)-N(1))-methyltransferase TrmI
Alanyl-transfer RNA synthetases family profile
Aromatic amino acid beta-eliminating lyase/threonine aldolase
Pyruvate ferredoxin oxidoreductase beta subunit C-terminal
CobB/CobQ-like glutamine amidotransferase
Increases nucleophilicity of active site Cys
Little change in the aminoacylation activity.
Slightly reduces the aminoacylation activity.
Schiff-base intermediate with acetaldehyde
DNA polymerase III beta sliding clamp C-terminal
DNA polymerase III beta sliding clamp N-terminal
DNA polymerase III beta sliding clamp central
Carboxypeptidase regulatory-like domain-containing protein
Probable glycine dehydrogenase (decarboxylating) subunit 2
Prepilin type IV endopeptidase peptidase
Ribosomal RNA small subunit methyltransferase F
Ribosomal RNA large subunit methyltransferase I
Involved in ionization of N3 of dUMP, leading to its activation
Cytochrome C biogenesis protein transmembrane
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Pyrimidine nucleoside phosphorylase C-terminal
Ribosomal RNA small subunit methyltransferase A
ATP phosphoribosyltransferase regulatory subunit
Alpha-glycerophosphate oxidase C-terminal
Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase
Ribosomal RNA small subunit methyltransferase H
Inner membrane protein YgaP-like transmembrane
Important for substrate specificity and discrimination against 3-isopropylmalate
4-hydroxy-4-methyl-2-oxoglutarate aldolase
4-hydroxyphenylacetate 3-monooxygenase oxygenase component
Ionotropic glutamate receptor C-terminal
Solute-binding protein family 3/N-terminal
Transcription termination/antitermination protein NusA
Phosphoribosylformylglycinamidine synthase subunit PurL
Electron transfer via tryptophanyl radical
Strongly reduces interaction with DNA. Reduces CPD repair activity by 80%.
Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal
Beta-ketoacyl-[acyl-carrier-protein] synthase III N-terminal
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Crossover junction endodeoxyribonuclease RuvC
Slightly reduced HJ resolution activity, altered sequence specificity.
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
Nicotinate phosphoribosyltransferase C-terminal
Nicotinate phosphoribosyltransferase N-terminal
3-isopropylmalate dehydratase large subunit
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
Uncharacterized PIN and TRAM-domain containing protein TTHA0540
Transcription termination/antitermination protein NusG
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
Sugar fermentation stimulation protein C-terminal
Polyribonucleotide nucleotidyltransferase
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Arsenite oxidase subunit AioA/Iodate reductase subunit IdrA 3Fe-4S cluster
Putative regulatory protein FmdB Zinc ribbon
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Positions MEP for the nucleophilic attack
2-oxoisovalerate dehydrogenase subunit alpha
Organic solvent tolerance-like N-terminal
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Ribosomal RNA small subunit methyltransferase E PUA-like
Ribosomal RNA small subunit methyltransferase E methyltransferase
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
64% loss of cleavage activity (PubMed:21572442); 99% loss of cleavage activity (PubMed:21572444).
99.8% loss of cleavage activity, binds RNA normally.
No effect on cleavage, increases enzyme turnover.
Stabilizes transition-state intermediate
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
3-isopropylmalate dehydratase small subunit
Cytochrome oxidase subunit II copper A binding
Cytochrome oxidase subunit II transmembrane region profile
Ribosomal protein uS12 methylthiotransferase RimO
Type III-B CRISPR module RAMP protein Cmr4
Quinolinate phosphoribosyl transferase C-terminal
Quinolinate phosphoribosyl transferase N-terminal
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
NAD(P) transhydrogenase alpha subunit C-terminal
5-glutamyl 2-aminoadipic acid; alternate
Disrupts lysine biosynthesis. Lysine-auxotrophic phenotype.
Glutamate-1-semialdehyde 2,1-aminomutase
tRNA (guanosine(18)-2'-O)-methyltransferase
Probable transcriptional regulatory protein TTHA0821
YbaK/aminoacyl-tRNA synthetase-associated
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in TtuB)
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Phenylalanine--tRNA ligase alpha subunit
Carbamoyl-phosphate synthase large chain
Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
[LysW]-aminoadipate semialdehyde transaminase
No effect on protease activity against alpha-casein.
DNA-directed RNA polymerase subunit beta'
Type II secretion system protein GspE N-terminal
Outer membrane protein beta-barrel domain-containing protein
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Interacts with tRNA; defines subfamily-specific binding signature