Abolishes peptidase activity, but retains ATPase activity.
Almost completely abolishes ATPase activity and severely reduces proteolytic activity.
Completely abolishes ATPase activity and makes peptidase activity independent on ATP stimulus.
Decreases ATPase activity. Completely abolishes peptidase activity in the absence of ATP, but ATP-stimulated peptidase activity i close to wild-type levels.
Retains 5% ATPase activity. Proteolytic activity is greatly impaired and cannot be stimulated by nucleotides.
Severely reduces ATPase and proteolytic activity.
Glutamyl-tRNA(Gln) amidotransferase subunit D
Bifunctional phosphopantetheine adenylyltransferase/NTP phosphatase
Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Glycosyltransferase subfamily 4-like N-terminal
UPF0173 metal-dependent hydrolase Ta0764
Uncharacterized solute-binding protein Ta1052
Major facilitator superfamily (MFS) profile
Probable transposase IS891/IS1136/IS1341
DNA repair and recombination protein RadB
Peptidase S9 prolyl oligopeptidase catalytic
Probable 2-phosphosulfolactate phosphatase
Translation initiation factor 2 subunit alpha
Aldehyde ferredoxin oxidoreductase N-terminal
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
DNA-directed RNA polymerase subunit Rpo10
Probable glycine dehydrogenase (decarboxylating) subunit 1
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
DNA double-strand break repair Rad50 ATPase
Thiamine pyrophosphate enzyme TPP-binding
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Tryptophan synthase beta chain-like PALP
SAM-dependent methyltransferase TRM5/TYW2-type
Putative nicotinate phosphoribosyltransferase
4.6-fold decrease in the catalytic efficiency for ATP, with only a marginal decrease in affinity compared to the wild-type.
Strong decrease of the affinity for ATP and 17-fold decrease of the catalytic efficiency compared to the wild-type.
Strong decrease of the affinity for ATP, but almost the same catalytic efficiency compared to the wild-type.
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
DNA-directed RNA polymerase subunit Rpo3
DNA-directed RNA polymerase subunit Rpo11
Ornithine carbamoyltransferase, catabolic
Digeranylgeranylglycerophospholipid reductase
Does not affect stimulation of helicase activity.
May have an important role in the acetylation of the polyamine
Glyceraldehyde-3-phosphate dehydrogenase
Phosphoribosylformylglycinamidine synthase subunit PurL
DNA-directed RNA polymerase subunit Rpo1C
Saccharopine dehydrogenase-like C-terminal
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
tRNA (cytidine(56)-2'-O)-methyltransferase
Glycogen debranching enzyme bacterial and archaeal type N-terminal
Ribosomal RNA small subunit methyltransferase Nep1
Glutamate-1-semialdehyde 2,1-aminomutase
Cobalt-precorrin-5B C(1)-methyltransferase
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Resolvase/invertase-type recombinase catalytic
Plays an important role in substrate specificity
Signal recognition particle 54 kDa protein
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Aminoacyl-transfer RNA synthetases class-II family profile
DNA/pantothenate metabolism flavoprotein C-terminal
Vitamin B12-dependent ribonucleoside-diphosphate reductase
Schiff-base intermediate with acetaldehyde
Nascent polypeptide-associated complex protein
Pyridoxal 5'-phosphate synthase subunit PdxT
Removed in mature form; by autocatalysis
Manganese/iron superoxide dismutase C-terminal
Manganese/iron superoxide dismutase N-terminal
Signal recognition particle receptor FtsY
Carbamoyl-phosphate synthase large chain
Potassium-transporting ATPase potassium-binding subunit
3-hydroxyacyl-CoA dehydrogenase C-terminal
3-hydroxyacyl-CoA dehydrogenase NAD binding
Tetrapyrrole biosynthesis uroporphyrinogen III synthase
Mandelate racemase/muconate lactonizing enzyme C-terminal
Aspartate/homoserine dehydrogenase NAD-binding
Extradiol ring-cleavage dioxygenase class III enzyme subunit B
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Both peptidolytic and proteolytic activities doubled, probably due to the increase of the diameter of the channel for product exit. Retains less than 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the exit channel and impairs product exit from the catalytic chamber.
Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-414.
Decreased catalytic activity towards protein substrates. Retains 10% of wild-type activity towards casein and about 30% towards oxidized insulin beta chain. However, there is no change in the activity for a tripeptide substrate.
Retains 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the access channel and impairs substrate access to the active site. Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation, probably due to the decrease of the diameter of the substrate access channel. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-643.
Transition state stabilizer; via amide nitrogen
DNA-directed RNA polymerase subunit Rpo5
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Cysteine sulfenic acid (-SOH) intermediate
tRNA (guanine(26)-N(2))-dimethyltransferase
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Translation initiation factor 2 subunit beta
Succinate dehydrogenase hydrophobic membrane anchor subunit
3-phosphoshikimate 1-carboxyvinyltransferase
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Adenosylcobinamide-GDP ribazoletransferase
GMP synthase [glutamine-hydrolyzing] subunit B
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Phosphoadenosine phosphosulphate reductase
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Uncharacterised membrane protein Ta0354 soluble
D-glyceraldehyde dehydrogenase (NADP(+))
Polysaccharide biosynthesis protein C-terminal
O-(5'-phospho-DNA)-tyrosine intermediate
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Glutamyl-tRNA(Gln) amidotransferase subunit E
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Mevalonate 3,5-bisphosphate decarboxylase
No effect. Strongly reduced ribonuclease activity; when associated with A-21.
No effect. Strongly reduced ribonuclease activity; when associated with A-22.
Putative sulfur carrier protein Ta1170/Ta1414
Phosphoribosylformylglycinamidine synthase subunit PurQ
Translation initiation factor 2 subunit gamma
Aspartate carbamoyltransferase regulatory chain
Formylmethanofuran dehydrogenase subunit E
DNA-directed RNA polymerase subunit Rpo6
Important for guanine/8-oxoguanine distinction
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
6-hydroxymethylpterin diphosphokinase MptE-like
DNA-directed RNA polymerase subunit Rpo1N
DNA-directed DNA polymerase family B exonuclease
DNA-directed DNA polymerase family B multifunctional
Probable glycine dehydrogenase (decarboxylating) subunit 2
Electron transfer flavoprotein alpha subunit C-terminal
Electron transfer flavoprotein alpha/beta-subunit N-terminal
Putative HTH-type transcriptional regulatory protein Ta1363
Iron dependent repressor metal binding and dimerisation
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
Pyruvate/ketoisovalerate oxidoreductase catalytic
GMP synthase [glutamine-hydrolyzing] subunit A
Probable cyclic pyranopterin monophosphate synthase
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
Markedly increases peptidolytic activity. Designated open-gate mutant.
No effect on PAN's ability to stimulate gate opening.
Prevents PAN to associate with the proteasome and stimulate gate opening.
Dinitrogenase iron-molybdenum cofactor biosynthesis
Increases nucleophilicity of active site Cys
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
DNA-directed RNA polymerase subunit Rpo2
Phosphoribosylformylglycinamidine cyclo-ligase
Probable translation initiation factor IF-2
Phosphoribosylaminoimidazole-succinocarboxamide synthase
O-(3'-phospho-DNA)-tyrosine intermediate
Ribosomal RNA large subunit methyltransferase E