Cytochrome b/b6 N-terminal region profile
Probable glycine cleavage system H protein 2
Nicotinamide-nucleotide adenylyltransferase
GMP synthase [glutamine-hydrolyzing] subunit A
Resolvase/invertase-type recombinase catalytic
5-glutamyl 2-aminoadipic acid; alternate
Alpha-aminoadipate/glutamate carrier protein LysW
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
Digeranylgeranylglyceryl phosphate synthase
Pyruvate/ketoisovalerate oxidoreductase catalytic
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Nitrite/Sulfite reductase ferredoxin-like
Cysteine sulfenic acid (-SOH) intermediate
Cytochrome oxidase subunit II copper A binding
Cobalamin-independent methionine synthase MetE N-terminal
Manganese/iron superoxide dismutase C-terminal
Manganese/iron superoxide dismutase N-terminal
Glyceraldehyde-3-phosphate dehydrogenase
Schiff-base intermediate with dihydroxyacetone-P
DNA-directed RNA polymerase M/15kDa subunit
Probable glycine dehydrogenase (decarboxylating) subunit 2
Major facilitator superfamily (MFS) profile
Bacterial bifunctional deaminase-reductase C-terminal
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Phosphoribosylformylglycinamidine cyclo-ligase
Electron transfer flavoprotein alpha/beta-subunit N-terminal
Nicotinate phosphoribosyltransferase C-terminal
Quinolinate phosphoribosyl transferase C-terminal
Glutamyl-tRNA(Gln) amidotransferase subunit D
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead
Glutamyl-tRNA(Gln) amidotransferase subunit E
Probable transposase IS891/IS1136/IS1341
Pyridine nucleotide-disulphide oxidoreductase dimerisation
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)
Phosphoglucosamine/phosphogalactosamine mutase
Ribosome maturation protein SDO1/SBDS C-terminal
Ribosome maturation protein SDO1/SBDS N-terminal
Ribosome maturation protein SDO1/SBDS central
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Kelch domain-containing protein STK_09390
Transcription regulator AsnC/Lrp ligand binding
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Proton acceptor; for processing activity
Schiff-base intermediate with substrate; via pyruvic acid
Glycosyl-hydrolase family 116 N-terminal
Glycosyl-hydrolase family 116 catalytic region
3-hydroxyacyl-CoA dehydrogenase C-terminal
3-hydroxyacyl-CoA dehydrogenase NAD binding
Peptidase S9 prolyl oligopeptidase catalytic
Arsenite oxidase subunit AioA/Iodate reductase subunit IdrA 3Fe-4S cluster
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Removed in mature form; by autocatalysis
Quinolinate phosphoribosyl transferase N-terminal
ATP-grasp fold succinyl-CoA synthetase-type
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
Important in orienting the L-aspartate substrate
Uncharacterized solute-binding protein STK_20340
Plays an important role in substrate specificity
Imidazole glycerol phosphate synthase subunit HisH
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
Cytochrome c-552/DMSO reductase-like haem-binding
Inosine/uridine-preferring nucleoside hydrolase
2-oxoacid:ferredoxin oxidoreductase 2, subunit beta
Plays an important role in the binding of CoA
Dinitrogenase iron-molybdenum cofactor biosynthesis
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Carbamoyl-phosphate synthase large chain
Signal recognition particle 54 kDa protein
Carbamoyl-phosphate synthase small chain
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Glycine cleavage T-protein C-terminal barrel
Pyruvate phosphate dikinase AMP/ATP-binding
[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase
DNA-directed RNA polymerase subunit Rpo12
Phenylalanine--tRNA ligase alpha subunit
DNA-directed RNA polymerase subunit Rpo10
Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop
Glycoside hydrolase family 57 N-terminal
Glycosyltransferase subfamily 4-like N-terminal
3-isopropylmalate dehydratase small subunit
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Transcription factor NikR nickel binding C-terminal
3-isopropylmalate dehydratase large subunit
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
CobB/CobQ-like glutamine amidotransferase
Probable translation initiation factor IF-2
Probable glycine cleavage system H protein 1
DNA-directed DNA polymerase family B exonuclease
DNA-directed DNA polymerase family B multifunctional
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
CRISPR-associated protein Cas6 C-terminal
Putative ABC transporter ATP-binding protein STK_11360
Increases nucleophilicity of active site Cys
DNA double-strand break repair helicase HerA
Lack of ATPase and helicase activities. Decreases ssDNA-binding ability. Does not bind to blunt-ended dsDNA and Holliday junction substrate.
Strong decrease in ATPase and helicase activities.
O-(3'-phospho-DNA)-tyrosine intermediate
DNA-directed RNA polymerase subunit Rpo3
Glycogen debranching enzyme bacterial and archaeal type N-terminal
3-methyl-2-oxobutanoate hydroxymethyltransferase
Glycoside-hydrolase family GH114 TIM-barrel
3-phosphoshikimate 1-carboxyvinyltransferase
Tetrapyrrole biosynthesis uroporphyrinogen III synthase
Myo-inositol-1-phosphate synthase GAPDH-like
Imidazole glycerol phosphate synthase subunit HisF
Glycoside hydrolase family 31 N-terminal
Phosphatidic acid phosphatase type 2/haloperoxidase
Glycosyl transferase family 28 C-terminal
Probable cyclic pyranopterin monophosphate synthase
Formate-dependent phosphoribosylglycinamide formyltransferase
Aspartate carbamoyltransferase regulatory chain
Mandelate racemase/muconate lactonizing enzyme C-terminal
Probable glycine cleavage system H protein 3
Threonine--tRNA ligase catalytic subunit
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Involved in ionization of N3 of dUMP, leading to its activation
10-fold reduction in both RNase H and dsRNase activity.
20% reduction in RNase H activity, 40% reduction in dsRNase activity, partial loss of thermostability.
Loss of RNase H activity, loss of dsRNase activity. Does not complement E.coli double deletion mutant.
RNase H and dsRNase activity increase slightly, decreased thermostability; when associated with A-145.
RNase H and dsRNase activity increase slightly, decreased thermostability; when associated with A-58.
Retains RNase H activity, considerable loss of dsRNase activity.
Retains RNase H activity, loss of dsRNase activity. Complements E.coli double deletion mutant.
DNA double-strand break repair protein Mre11
Phosphoadenosine phosphosulphate reductase
Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase
Decrease in GalN-1-PAcTase activity and increase in GlcN-1-PAcTase activity.
Decrease in GlcNAc-1-PUTase activity and loss of Glc-1-PUTase activity.
Decrease in GlcNAc-1-PUTase activity but increase in Glc-1-PUTase activity.
Decrease in GlcNAc-1-PUTase and Glc-1-PUTase activities.
Decreases GlcNAc-1-PUTase and Glc-1-PUTase activities.
Increases GlcNAc-1-PUTase activity. Decreases Glc-1-PUTase activity.
Increases GlcNAc-1-PUTase and Glc-1-PUTase activities.
Increases both GlcNAc-1-PUTase and Glc-1-PUTase activities.
Loss of GlcNAc-1-PUTase activity; when associated with N-80.
Loss of GlcNAc-1-PUTase activity; when associated with V-97.
Loss of both GlcNAc-1-PUTase and Glc-1-PUTase activities.
No change in GlcNAc-1-PUTase activity. Shows 20% less GalN-1-PAcTase activity than the wild-type, but increases GlcN-1-PAcTase activity 4.8 times. Does not affect thermostability.
No change in GlcNAc-1-PUTase activity. Shows 38% less GalN-1-PAcTase activity than the wild-type, but increases GlcN-1-PAcTase activity 16.8 times. Significantly affects the thermostability of the entire protein.
Slight decrease in GalN-1-PAcTase activity and increase in GlcN-1-PAcTase activity.
Strong decrease in GalN-1-PAcTase activity and almost loss of GlcN-1-PAcTase activity.
Strong decrease in GalN-1-PAcTase activity and decrease in GlcN-1-PAcTase activity.
Strong decrease in GalN-1-PAcTase activity and increase in GlcN-1-PAcTase activity.
Strong decrease in GlcNAc-1-PUTase activity and loss of Glc-1-PUTase activity.
Strong increase in GlcNAc-1-PUTase activity and decrease in Glc-1-PUTase activity.
[LysW]-aminoadipate/[LysW]-glutamate kinase
GF motif that is essential for ArgX substrate specificity
Putative pterin-4-alpha-carbinolamine dehydratase
Probable inosine/xanthosine triphosphatase
1.3-fold increase in FBPase catalytic efficiency.
1.7-fold increase in FBPase catalytic activity and 5-fold decrease in FBP affinity, leading to a 2.7-fold decrease in FBPase catalytic efficiency.
1000-fold decrease in FBPase catalytic activity.
175-fold decrease in FBPase catalytic efficiency.
2-fold increase in FBP affinity and 10-fold decrease in FBPase catalytic activity, leading to a 5.5-fold decrease in FBPase catalytic efficiency.
2000-fold decrease in FBPase catalytic activity.
275-fold decrease in FBPase catalytic activity.
930-fold decrease in FBPase catalytic efficiency.
Fructose-1,6-bisphosphate aldolase/phosphatase
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Retains the FBPase activity, but loses the FBP aldolase activity.
Schiff-base intermediate with DHAP; for FBP aldolase activity
Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)
tRNA N6-adenosine threonylcarbamoyltransferase
Transposase IS116/IS110/IS902 C-terminal
DNA-directed RNA polymerase subunit 2 hybrid-binding
Aldehyde oxidase/xanthine dehydrogenase second molybdopterin binding
Polymerase/histidinol phosphatase N-terminal
DNA-directed RNA polymerase subunit Rpo11
Bacterial type II secretion system protein E
Cytochrome b/b6 C-terminal region profile
Ribosomal RNA adenine methylase transferase N-terminal
Aspartate/homoserine dehydrogenase NAD-binding
Plays an important role in modulating multiple conformations of loop regions and contributes to protein thermostability
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Docks into pockets in the proteasome alpha-ring to cause gate opening
2-oxoacid:ferredoxin oxidoreductase 1, subunit beta
Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
Same oxidoreductase activity as the wild-type.
Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
Electron transfer via tryptophanyl radical
SAM-dependent methyltransferase TRM5/TYW2-type
Tryptophan synthase beta chain-like PALP
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
Translation initiation factor 2 subunit gamma
2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
DNA-directed RNA polymerase subunit Rpo5
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
N-[TS] motif that is essential for LysX substrate specificity
Phosphoribosylformylglycinamidine synthase subunit PurL
Membrane-associated ATPase epsilon chain
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Methylated-DNA--protein-cysteine methyltransferase
Gamma-glutamylcyclotransferase AIG2-like
UPF0173 metal-dependent hydrolase STK_14180
DNA double-strand break repair nuclease NurA
Strong decrease in nuclease activity. Does not form a stable dimer.
CRISPR system ring nuclease SSO1393-like
Anthranilate synthase component I N-terminal
Thiosulphate:quinone oxidoreductase small subunit DoxA
Probable glycine dehydrogenase (decarboxylating) subunit 1
Ribosomal RNA small subunit methyltransferase Nep1
3-hydroxypropionyl-coenzyme A synthetase
Aminoacyl-transfer RNA synthetases class-II family profile
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
Glutamyl-tRNA(Gln) amidotransferase subunit A
Glutamyl-tRNA(Gln) amidotransferase subunit C
Large ribosomal subunit protein uL15/eL18
[LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
D-galactarate/Altronate dehydratase C-terminal
Pyridoxal 5'-phosphate synthase subunit PdxT
Important for guanine/8-oxoguanine distinction
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
Putative Tricorn-like protease C-terminal subunit
Transition state stabilizer; via amide nitrogen
Adenosylcobinamide-GDP ribazoletransferase
Translation initiation factor 2 subunit alpha
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
DNA double-strand break repair Rad50 ATPase
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Sugar fermentation stimulation protein homolog
Metallo-beta-lactamase fold-containing protein ST1585
GMP synthase [glutamine-hydrolyzing] subunit B
DNA-directed RNA polymerase subunit Rpo1C
tRNA (guanine(26)-N(2))-dimethyltransferase
SUF system FeS cluster assembly SufBD N-terminal
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
[LysW]-lysine/[LysW]-ornithine hydrolase
Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose