RNA 2-O ribose methyltransferase substrate binding
Major facilitator superfamily (MFS) profile
NADH-rubredoxin oxidoreductase C-terminal
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Probable glycine dehydrogenase (decarboxylating) subunit 2
Polysaccharide chain length determinant N-terminal
O-(5'-phospho-DNA)-tyrosine intermediate
Na+/H+ antiporter MnhB subunit-related protein
ATP-dependent Clp protease ATP-binding subunit ClpX
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Phosphate import ATP-binding protein PstB
S-adenosylmethionine-dependent methyltransferase
Ysc84 actin-binding domain-containing protein
3-isopropylmalate dehydratase large subunit 1
Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha
Large ribosomal subunit protein uL2 C-terminal
Probable transposase IS891/IS1136/IS1341
Polymerase/histidinol phosphatase N-terminal
Energy-coupling factor transporter ATP-binding protein EcfA2
Alpha-D-phosphohexomutase alpha/beta/alpha
4-hydroxy-tetrahydrodipicolinate reductase
Dihydrodipicolinate reductase C-terminal
Dihydrodipicolinate reductase N-terminal
11% DNA-dependent ATPase activity, allows branch migration.
3-4% DNA-dependent ATPase activity, nobranch migration.
32% DNA-dependent ATPase activity, allows branch migration.
43% DNA-dependent ATPase activity, allows branch migration.
5% DNA-dependent ATPase activity, no branch migration.
Holliday junction branch migration complex subunit RuvB
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
Flagellar basal body rod protein N-terminal
Ribosome maturation factor RimP C-terminal
Ribosome maturation factor RimP N-terminal
Probable metal transport system ATP-binding protein TM_0124
Glycoside hydrolase family 42 N-terminal
Sec translocon accessory complex subunit YajC
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
Bifunctional purine biosynthesis protein PurH
tRNA pseudouridylate synthase B C-terminal
Ribosomal RNA adenine methylase transferase N-terminal
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Positions MEP for the nucleophilic attack
DNA-directed DNA polymerase family A palm
Redox-sensing transcriptional repressor Rex 1
Large ribosomal subunit protein uL6 alpha-beta
Binds dUMP 670-fold weaker than wild-type.
Involved in ionization of N3 of dUMP, leading to its activation
Still catalytically active although only shows 0.0008% of wild-type activity. Binds dUMP 7300-fold weaker than wild-type.
Still catalytically active although shows a large decrease in activity.
Thiamin pyrophosphokinase thiamin-binding
Probable septum site-determining protein MinC
Septum formation inhibitor MinC C-terminal
Decreased iron content and loss of activity; when associated with A-150 and A-154.
Decreased iron content and loss of activity; when associated with A-150 and A-157.
Decreased iron content and loss of activity; when associated with A-154 and A-157.
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Aminoacyl-transfer RNA synthetases class-II family profile
Cytochrome C biogenesis protein transmembrane
Large ribosomal subunit protein bL12 C-terminal
Large ribosomal subunit protein bL12 oligomerization
Phospho-2-dehydro-3-deoxyheptonate aldolase
HicB-like antitoxin of toxin-antitoxin system
Aromatic amino acid beta-eliminating lyase/threonine aldolase
UPF0173 metal-dependent hydrolase TM_1162
Phenylalanine--tRNA ligase alpha subunit
Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic
Uncharacterized HTH-type transcriptional regulator TM_0326
Anthranilate synthase component I N-terminal
3-isopropylmalate dehydratase small subunit 2
Flagellar hook-associated protein 2 C-terminal
Flagellar hook-associated protein 2 N-terminal
Small ribosomal subunit biogenesis GTPase RsgA
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
Involved in allosteric activation by GTP
Prokaryotic-type class I peptide chain release factors
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding
Important for guanine/8-oxoguanine distinction
Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Release factor glutamine methyltransferase
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Probable transcriptional regulatory protein TM_0466
Phosphoribosylformylglycinamidine synthase subunit PurQ
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Flagellar motor switch protein FliN-like C-terminal
No appreciable effect on the binding affinity for CheY.
Loss of activity. Does not prevent binding to mcp2.
N-(5'-phosphoribosyl)anthranilate isomerase
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
Proton acceptor; for glutaminase activity
Thiamine pyrophosphate enzyme TPP-binding
tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain
Energy-coupling factor transporter ATP-binding protein EcfA1
Aspartate/ornithine carbamoyltransferase Asp/Orn-binding
Aspartate/ornithine carbamoyltransferase carbamoyl-P binding
Carbamoyl-phosphate synthase small chain
Carbamoyl-phosphate synthase small subunit N-terminal
Ribose import ATP-binding protein RbsA 2
Reversibly protonated during proton transport
Ribonucleotide reductase large subunit C-terminal
Ribonucleotide reductase large subunit N-terminal
Could be important to modulate the pK values of the two catalytic cysteine residues
Protein-glutamate methylesterase/protein-glutamine glutaminase
Glutamate synthase alpha subunit C-terminal
Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase
Shikimate dehydrogenase substrate binding N-terminal
S-adenosyl-L-homocysteine hydrolase NAD binding
DNA-directed RNA polymerase RpoA/D/Rpb3-type
DNA-directed RNA polymerase subunit alpha
Transcription termination/antitermination protein NusG
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Heat-inducible transcription repressor HrcA
Heat-inducible transcription repressor HrcA C-terminal
Cleaves more rapidly than the wild-type.
Cleaves much more slowly than the wild-type, but the addition of hydroxylamine which is known to cleave ester bonds leads to the cleavage of this mutant.
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Mandelate racemase/muconate lactonizing enzyme C-terminal
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Phosphate-specific transport system accessory protein PhoU homolog 1
Prepilin type IV endopeptidase peptidase
Survival protein SurE-like phosphatase/nucleotidase
Helix-hairpin-helix DNA-binding motif class 1
ATP-dependent protease ATPase subunit HslU
Putative 1-aminocyclopropane-1-carboxylate deaminase
Tryptophan synthase beta chain-like PALP
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like
Flagellar hook-basal body complex protein FliE
Glycosyl transferase family 28 C-terminal
Glycosyltransferase family 28 N-terminal
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Flagellar basal-body/hook protein C-terminal
N-acetyl-gamma-glutamyl-phosphate reductase
Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
Glyceraldehyde-3-phosphate dehydrogenase
ATP synthase F1 complex delta/epsilon subunit N-terminal
Electron transfer flavoprotein alpha/beta-subunit N-terminal
Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding
Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic
Large ribosomal subunit protein uL5 C-terminal
Large ribosomal subunit protein uL5 N-terminal
Interaction with polymerase core subunit RpoC
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Pyruvate ferredoxin oxidoreductase beta subunit C-terminal
Lysidine-tRNA(Ile) synthetase C-terminal
Pseudouridine synthase I TruA alpha/beta
Alanyl-transfer RNA synthetases family profile
Abolishes pentamerization. Abolishes ion transport.
Abolishes pentamerization. Mildly decreases ion transport.
Abolishes pentamerization. Strongly decreases ion transport.
Impairs pentamerization. Decreases ion transport.
Important for closing the ion permeation pathway in the closed state
Increases ion transport by suppression of a kink in the transmembrane ion permeation pathway.
Increases ion transport by suppression of an obstruction in the transmembrane ion permeation pathway.
Increases protein stability. Decreases ion transport.
No effect on pentamerization. Abolishes ion transport.
No effect on pentamerization. Decreases ion transport.
No effect on pentamerization. Increases ion transport.
No effect on pentamerization. No effect on ion transport.
No significant decrease of Co(2+) uptake, but abolishes selectivity for Co(2+).
Reduces Co(2+) uptake. Abolishes Co(2+) uptake; when associated with L-295.
Strongly reduces Co(2+) uptake. Abolishes Co(2+) uptake; when associated with L-299.
Threonine that confers selectivity for Co(2+) transport
Ribose-phosphate pyrophosphokinase N-terminal
Energy-coupling factor transporter transmembrane protein EcfT
Flagellar motor switch protein FliG C-terminal
Flagellar motor switch protein FliG N-terminal
Flagellar motor switch protein FliG middle
Part of the EHPQR-motif; important for interaction with FliM
DNA-directed RNA polymerase subunit beta'
ATP-dependent Clp protease proteolytic subunit
tRNA (guanine-N(1)-)-methyltransferase C-terminal
Membrane fusion protein biotin-lipoyl like
Glycoside hydrolase family 31 N-terminal
Chromosomal replication initiator DnaA C-terminal
Chromosomal replication initiator protein DnaA
Flagellar Assembly Protein A N-terminal region
Probable nicotinate-nucleotide adenylyltransferase
Bifunctional thiamine biosynthesis protein ThiDN
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
S-adenosylmethionine synthetase C-terminal
S-adenosylmethionine synthetase N-terminal
Putative regulatory protein FmdB Zinc ribbon
Bifurcating [FeFe] hydrogenase beta subunit
tRNA N6-adenosine threonylcarbamoyltransferase
NADH:ubiquinone oxidoreductase 30kDa subunit
Imidazole glycerol phosphate synthase subunit HisH
Trigger factor ribosome-binding bacterial
Flagellar hook-length control protein-like C-terminal
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
tRNA-5-methyluridine(54) 2-sulfurtransferase
Has little effects on the NTPase activity.
Nucleoside triphosphate pyrophosphohydrolase/pyrophosphatase MazG
Reduces the NTPase activity to 10% of the wild-type activity.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with A-97.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with A-98.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with Q-41.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with Q-42.
Ribosomal RNA small subunit methyltransferase H
Aminoacyl-tRNA synthetase class I anticodon-binding
Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic
Participates in a stacking interactions with the sugar rings of 4-O-MeGlcA
Sodium/calcium exchanger membrane region
Solute-binding protein family 3/N-terminal
TRAP C4-dicarboxylate transport system permease DctM subunit
Type III secretion system flagellar brake protein YcgR PilZN
Carbamoyl-phosphate synthase large chain
Bacterial type II secretion system protein E
Glycoside-hydrolase family GH114 TIM-barrel
SUF system FeS cluster assembly SufBD N-terminal
Damage-control phosphatase ARMT1-like metal-binding
Polyribonucleotide nucleotidyltransferase
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Pyruvate/ketoisovalerate oxidoreductase catalytic
S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal
S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal
Large ribosomal subunit protein uL30-like ferredoxin-like fold
Formiminotransferase C-terminal subdomain
Formiminotransferase N-terminal subdomain
Myo-inositol-1-phosphate synthase GAPDH-like
Probable 2-phosphosulfolactate phosphatase
Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich
Glycosyl hydrolases family 2 sugar binding
DNA mismatch repair proteins mutS family
Transcription elongation factor GreA/GreB C-terminal
Transcription elongation factor GreA/GreB N-terminal
Loss of activity; when associated with R-219.
No change in activity. Retains weak activity; when associated with F-21. Loss of activity; when associated with F-107.
Retains weak activity; when associated with R-219.
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
Putative membrane protein insertion efficiency factor
Able to regulate the E.coli S10 operon, but does not improve association with E.coli ribosome; when associated with E141Q, Q144K, Q147A, S149E, K151delta, K152delta, I155M, P158G, W159E and K160L.
Considerably improves stable incorporation into E.coli ribosomes, however no change in regulation of the E.coli S10 operon.
Little change in either regulation of the E.coli S10 operon or ribosome assembly.
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Loss of flavin-binding. No change in iron storage or iron release under aerobic or anaerobic conditions.
Makes normal nanocompartments, pore size increases to 10.1 A, considerably increased Cl(-) flux.
Makes normal nanocompartments, pore size increases to 10.7 A, slightly increased Cl(-) flux.
Makes normal nanocompartments, pore size increases to 11.3 A. Increased metabolic flux across the pore.
Makes normal nanocompartments, pore size increases to 5.5 A, no change in Cl(-) flux.
Makes normal nanocompartments, pore size increases to 6.7 A, considerably increased Cl(-) flux.
Makes normal nanocompartments, pore size increases to about 5 A.
Makes normal nanocompartments, pore size increases to about 6 A.
Makes normal nanocompartments, pore size increases up to 4.0 A, slightly increased Cl(-) flux.
Makes normal nanocompartments, pore size remains 3 A.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Penicillin-binding protein transpeptidase
ABC transporter substrate-binding protein PnrA-like
Highly reduced activity. 10-fold reduction of the binding affinity for NAD(+). No change in substrate affinity.
No change in activity and substrate affinity. 5-fold reduction of the binding affinity for NAD(+).
Reduced activity. 300-fold reduction of the binding affinity for NAD(+). No change in substrate affinity.
Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
Sugar fermentation stimulation protein C-terminal
Sugar fermentation stimulation protein homolog
Glucose-6-phosphate dehydrogenase C-terminal
Glucose-6-phosphate dehydrogenase NAD-binding
Significant decrease of the kinase activity with a 17-fold decrease of catalytic efficiency.
Significant decrease of the kinase activity with a 180-fold decrease of catalytic efficiency.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Probable glycine dehydrogenase (decarboxylating) subunit 1
Pyridoxal 5'-phosphate synthase subunit PdxT
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Probable dual-specificity RNA methyltransferase RlmN
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
tRNA-specific 2-thiouridylase MnmA-like C-terminal
tRNA-specific 2-thiouridylase MnmA-like central
Transcription antitermination protein NusB
Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-67.
Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-70.
Eliminates binding of one iron-sulfur cluster; when associated with A-67 and A-70.
[FeFe] hydrogenase maturase subunit HydE
Probable multidrug resistance protein NorM
Glutamyl-tRNA(Gln) amidotransferase subunit C
Important in orienting the substrate; probably interacts with magnesium or manganese
Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
200-fold decrease in catalytic efficiency. Loss of activity; when associated with A-422.
300-fold decrease in catalytic efficiency.
300-fold decrease in catalytic efficiency. Loss of activity; when associated with A-310.
Bifurcating [FeFe] hydrogenase gamma subunit
Decrease (22 mV) in the redox potential.
Decrease (46 mV) in the redox potential.
Decrease (about 37-50 mV) in the redox potential.
Increase (30 mV) in the redox potential.
Increase (about 23-33 mV) in the redox potential.
Large decrease (132 mV) in the redox potential.
Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
Large decrease (65 mV) in the redox potential.
Large increase (85 mV) in the redox potential.
Small decrease (about 4-20 mV) in the redox potential.
Small increase (11 mV) in the redox potential.
Small increase (about 2-14 mV) in the redox potential.
Large ribosomal subunit protein bL25 L25
Large ribosomal subunit protein bL25 beta
DNA-directed RNA polymerase subunit beta
Schiff-base intermediate with acetaldehyde
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Probable branched-chain-amino-acid aminotransferase
Putative UDP-N-acetylglucosamine 2-epimerase
Probable DNA double-strand break repair Rad50 ATPase
DNA-directed RNA polymerase subunit omega
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--LD-lysine ligase
Complete loss of protease activity and of oligomerization.
NIF system FeS cluster assembly NifU N-terminal
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Membrane insertase YidC/Oxa/ALB C-terminal
Organic solvent tolerance-like N-terminal domain-containing protein
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Cobalamin-independent methionine synthase MetE C-terminal/archaeal
Cobalamin-independent methionine synthase MetE N-terminal
Polysaccharide biosynthesis protein CapD-like
Plays an important role in substrate specificity
3-oxoacyl-[acyl-carrier-protein] reductase FabG
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Galactose-1-phosphate uridyl transferase C-terminal
Galactose-1-phosphate uridyl transferase N-terminal
Galactose-1-phosphate uridylyltransferase
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Polysaccharide biosynthesis protein C-terminal
1500-fold lower catalytic rate and 1000-fold lower catalytic efficiency than the wild-type enzyme with p-nitrophenyl-alpha-D-galactopyranoside as substrate.
Between 200 and 800-fold lower catalytic rate and between 300 and 1700-fold lower catalytic efficiency than the wild-type enzyme with aryl-alpha-galactosides as substrates.
Increased transglycosylating activity at high concentrations of p-nitrophenyl-alpha-D-galactopyranoside substrate, which could be useful in industry and medicine for the synthesis of different p-nitrophenyl-digalactosides.
Increased transglycosylating activity at high concentrations of p-nitrophenyl-alpha-D-galactopyranoside substrate, which could be useful in industry and medicine for the synthesis of different p-nitrophenyl-digalactosides. Able to produce 16 times more of a regio-isomer with the (alpha1,2)-bond than wild-type enzyme.
Less than 1% of the wild-type enzyme activity with p-nitrophenyl-alpha-D-galactopyranoside as substrate at 80 degrees Celsius.
Reduced activity compared to the wild-type enzyme.
Cysteine sulfenic acid (-SOH) intermediate
Interchain (with Cys-211); in linked form
Interchain (with Cys-48); in linked form
D-glutamate N-acetyltransferase-like C-terminal
D-glutamate N-acetyltransferase-like N-terminal
Phosphoribosylformylglycinamidine synthase subunit PurS
Glycosyltransferase subfamily 4-like N-terminal
DNA polymerase III beta sliding clamp C-terminal
DNA polymerase III beta sliding clamp N-terminal
DNA polymerase III beta sliding clamp central
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Ribosomal RNA small subunit methyltransferase I
ATP phosphoribosyltransferase regulatory subunit
1-deoxy-D-xylulose 5-phosphate reductoisomerase
1-deoxy-D-xylulose 5-phosphate reductoisomerase C-terminal
1-deoxy-D-xylulose 5-phosphate reductoisomerase N-terminal
3-isopropylmalate dehydratase small subunit 1
Aconitase A/isopropylmalate dehydratase small subunit swivel
4-hydroxy-tetrahydrodipicolinate synthase
Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Iron dependent repressor metal binding and dimerisation
3-isopropylmalate dehydratase large subunit 2
Aspartate carbamoyltransferase regulatory region
Aspartate carbamoyltransferase regulatory subunit C-terminal
Aspartate carbamoyltransferase regulatory subunit N-terminal
Ribosomal RNA small subunit methyltransferase E PUA-like
Ribosomal RNA small subunit methyltransferase E methyltransferase
Spermidine/putrescine import ATP-binding protein PotA
Aspartate/homoserine dehydrogenase NAD-binding
RNA polymerase sigma factor 70 region 4 type 2
Dinitrogenase iron-molybdenum cofactor biosynthesis
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal
Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal
3-methyl-2-oxobutanoate hydroxymethyltransferase
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Activity abolished towards fluoroscein diacetoxymethyl ether.
Greater than 10-fold reduction in catalytic efficiency toward the acetate substrates. Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-228.
Minimal change in catalytic efficiency toward acetate substrates.
Minimal change in catalytic efficiency toward the acetate substrates.
Reduces catalytic activity by 33-fold against fluoroscein diacetoxymethyl ether; 8-fold decrease in activity against dimethacryloxymethyl ether; 4-fold increase in catalytic efficiency toward dicyclobutylcarboxymethyl ether. Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-92; A-213 or A-276.
Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-228.
Sigma 54 modulation/S30EA ribosomal protein C-terminal
Exonuclease VII large subunit C-terminal
Glucosamine/galactosamine-6-phosphate isomerase
No effect on DNA binding. No effect on cleavage of DNA containing inosine. Strongly reduced cleavage at apurinic sites.
No effect on cleavage of DNA containing inosine.
Reduced affinity for DNA. No effect on cleavage of DNA containing inosine. Abolishes cleavage at apurinic sites.
Strongly reduced cleavage of DNA containing inosine.
Glycine cleavage T-protein C-terminal barrel
Holliday junction branch migration complex subunit RuvA
UDP-glucose/GDP-mannose dehydrogenase C-terminal
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Uncharacterized ABC transporter ATP-binding protein TM_0352
Bifurcating [FeFe] hydrogenase alpha subunit
N5-carboxyaminoimidazole ribonucleotide mutase
NADPH-dependent 7-cyano-7-deazaguanine reductase
Archaeal-type glutamate synthase [NADPH]
Ribosomal RNA large subunit methyltransferase H
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
Redox-sensing transcriptional repressor Rex 2
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Involved in Mg(2+) ion dislocation from EF-Tu
Translation elongation factor EFTs/EF1B dimerisation
Ionotropic glutamate receptor C-terminal
Small ribosomal subunit protein uS4 N-terminal
Could play a key role in the communication between the regulatory and the substrate sites
Putative anti-sigma factor antagonist TM_1081
6-phosphogluconate dehydrogenase C-terminal
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Protein-L-isoaspartate O-methyltransferase
Imidazole glycerol phosphate synthase subunit HisF
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-112. In presence of CheD, reduced activity but exceeds activity of CheC alone; when associated with S-112.
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-115. In presence of CheD, almost no activity; when associated with S-115.
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-13. In presence of CheD, reduced activity but exceeds activity of CheC alone; when associated with S-13.
Loss of activity, in absence of CheD; in presence of CheD, reduced activity. Loss of activity, in absence of CheD; when associated with S-16. In presence of CheD, almost no activity; when associated with S-16.
Phosphate-specific transport system accessory protein PhoU homolog 2
RNA binding; important for wobble base 34 recognition
Uncharacterized ABC transporter ATP-binding protein TM_0288
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
GDP-mannose:di-myo-inositol-1,3'-phosphate beta-1,2-mannosyltransferase
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
5-methylthioadenosine/S-adenosylhomocysteine deaminase
Phosphorylated carbohydrates phosphatase TM_1254
Quinolinate phosphoribosyl transferase C-terminal
Quinolinate phosphoribosyl transferase N-terminal
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
Ribosomal protein uS12 methylthiotransferase RimO
K(+)-stimulated pyrophosphate-energized sodium pump
Silences the K(+)-independent activating Na(+)-binding site.
Putative anti-sigma factor antagonist TM_1442
Flagellar assembly protein FliH/Type III secretion system HrpE
200-fold decrease of the catalytic efficiency for FGAR. It has no effect on the ATP binding site, however it affects binding of FGAR.
Phosphoribosylformylglycinamidine synthase subunit PurL
Strong decrease of the binding affinity and 20-fold decrease of the catalytic efficiency for FGAR. It has no effect on the ATP binding site, however it affects binding of FGAR.
Glycoside hydrolase family 38 N-terminal
Pyruvate phosphate dikinase AMP/ATP-binding
FAD-dependent oxidoreductase 2 FAD binding
Translation elongation factor P/YeiP central
Uncharacterized RNA methyltransferase TM_1094
Large ribosomal subunit protein uL11 C-terminal
Large ribosomal subunit protein uL11 N-terminal
Large ribosomal subunit protein uL15/eL18
Ribose import ATP-binding protein RbsA 1
Bifunctional shikimate kinase/3-dehydroquinate synthase
Gly-cisPro motif, important for rejection of L-amino acids
Putative dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Fe-S hydro-lyase tartrate dehydratase beta-type catalytic
Probable glycerol uptake facilitator protein
Pyrimidine nucleoside phosphorylase C-terminal
ATPase F1/V1/A1 complex alpha/beta subunit N-terminal
ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding