SPbeta prophage-derived uncharacterized protein YomQ
Menaquinol:cytochrome c reductase iron-sulfur subunit
Abolishes induction of sigma-W-controlled genes.
No longer requires PrsW for degradation.
Partially inhibits SigW, confers low diamide induction. A swap mutant with RsrA of S.coelicolor.
Retains anti-sigma-W activity, not degraded by PrsW.
Wild-type; retains anti-sigma-W activity, degraded by PrsW.
Significant decrease of isomerase activity.
Important for interaction with partner proteins
Major facilitator superfamily (MFS) profile
Uncharacterized MFS-type transporter YitG
Phosphatidylglycerol lysyltransferase C-terminal
Uncharacterized MFS-type transporter YbfB
Putative membrane protein insertion efficiency factor
Phosphoribosylformylglycinamidine cyclo-ligase
Putative ABC transporter substrate-binding lipoprotein YvgL
Coenzyme A biosynthesis bifunctional protein CoaBC
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Spore coat polysaccharide biosynthesis protein SpsD
UPF0735 ACT domain-containing protein YszB
Uncharacterized MFS-type transporter YfnC
Retains NADPH-dependent oxidase activity under aerobic conditions, but at a lower level. No effect on S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-122 or with A-122 and A-220. Loss of bacilliredoxin reductase activity; when associated with A-122 and A-220.
Retains NADPH-dependent oxidase activity under aerobic conditions. Loss of S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-14 or with A-14 and A-122. Loss of bacilliredoxin reductase activity; when associated with A-14 and A-122.
Retains NADPH-dependent oxidase activity under aerobic conditions. No effect on S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-14 or with A-14 and A-220. Loss of bacilliredoxin reductase activity; when associated with A-14 and A-220.
SPbeta prophage-derived uncharacterized protein YomH
Thiamin pyrophosphokinase thiamin-binding
Putative carbonic anhydrase-like protein YbcF
Glucose-1-phosphate thymidylyltransferase
Ribosomal RNA small subunit methyltransferase G
Carbamoyl-phosphate synthase pyrimidine-specific small chain
Carbamoyl-phosphate synthase small subunit N-terminal
Ribosomal large subunit pseudouridine synthase B
Uncharacterized HTH-type transcriptional regulator YceK
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Putative 2-hydroxyacid dehydrogenase YoaD
NADPH-dependent 7-cyano-7-deazaguanine reductase
SPbeta prophage-derived uncharacterized protein YokK
Uncharacterized metallophosphoesterase YunD
Putative ADP-ribose pyrophosphatase YjhB
SPbeta prophage-derived uncharacterized protein YotF
Ribosome maturation factor RimP C-terminal
Ribosome maturation factor RimP N-terminal
Glycosyl transferase family 3 N-terminal
Partially restores cytochrome c synthesis in a CcdA-deficient mutant, possibly because the bacteria can no longer oxidize the 2 heme-binding thiol groups in apocytochrome c.
Putative metabolite transport protein YfiG
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
N-acetyl-gamma-glutamyl-phosphate reductase
Uncharacterized glycosyltransferase YwdF
Probable spore germination protein GerPC
Important for closing the ion permeation pathway in the closed state
Probable xanthine dehydrogenase subunit E
Uncharacterized ABC transporter ATP-binding protein YfmM
SPbeta prophage-derived disulfide bond formation protein A
Alkaline phosphatase synthesis sensor protein PhoR
Cell division topological determinant MinJ
Extracellular endo-alpha-(1->5)-L-arabinanase 1
Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate
Significantly less active (10000-fold) than the wild-type arabinanase against both linear arabinan and arabinooligosaccharides.
Significantly less active than the wild-type arabinanase against both linear arabinan (50-fold) and the arabinooligosaccharides (1000-fold).
Iron(3+)-hydroxamate import ATP-binding protein FhuC
No longer prevents EndoA toxicity upon coexpression in E.coli, due to loss of EndoA-EndoAI interaction.
Still prevents EndoA toxicity upon coexpression in E.coli.
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
Greater than wild-type diphosphate release.
Resolvase/invertase-type recombinase catalytic
Uncharacterized RNA pseudouridine synthase YhcT
Bacterial transcription activator effector binding
SPbeta prophage-derived putative transcriptional regulator YosT
Tryptophan RNA-binding attenuator protein
Uncharacterized HTH-type transcriptional regulator YvaF
Putative membrane-bound acyltransferase YfiQ
SPbeta prophage-derived uncharacterized protein YonH
Uncharacterized N-acetyltransferase YhdJ
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
DNA polymerase III beta sliding clamp C-terminal
DNA polymerase III beta sliding clamp N-terminal
DNA polymerase III beta sliding clamp central
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Resistance to polyketide antibiotic thailandamide.
Resistant to the pyrrolidine dione antibiotic CPD2.
Uncharacterized HTH-type transcriptional regulator YvaP
Penicillin-binding protein transpeptidase
Choline transport system permease protein OpuBB
Uncharacterized HTH-type transcriptional regulator YcgK
Peptidoglycan-N-acetylmuramic acid deacetylase PdaA
Alpha-D-phosphohexomutase alpha/beta/alpha
Impaired enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
Loss of enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
Very low enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
RNA binding; important for wobble base 34 recognition
Uncharacterized ABC transporter ATP-binding protein YtlC
Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
Glyceraldehyde-3-phosphate dehydrogenase 2
Phosphoribosylformylglycinamidine synthase subunit PurQ
No or little effects on GlcNAc6P binding.
Probable 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
In ts-A 73; temperature-sensitive lethal mutation.
Ribonucleoside-diphosphate reductase subunit alpha
10-fold reduction in glutaminase activity.
3-fold reduction in glutaminase activity.
Almost no effect on glutaminase activity.
No activity, disturbing interaction with PdxS.
Pyridoxal 5'-phosphate synthase subunit PdxT
Dramatically reduced endoribonuclease activity (0.22% of wild-type).
Dramatically reduced endoribonuclease activity (0.26% of wild-type).
Reduced endoribonuclease activity (11.1% of wild-type).
SPbeta prophage-derived uncharacterized protein YosH
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
SPbeta prophage-derived uncharacterized protein YotN
Negative regulator of flagellin synthesis
Glycine betaine/carnitine/choline transport system permease protein OpuCB
At least 50-fold decrease of the second-order rate constant for the acylation step.
No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
Pyruvate dehydrogenase E1 component subunit beta
Uncharacterized HTH-type transcriptional regulator YdeE
Galactose-1-phosphate uridyl transferase C-terminal
Galactose-1-phosphate uridyl transferase N-terminal
Galactose-1-phosphate uridylyltransferase
3-oxoacyl-[acyl-carrier-protein] synthase 2
In strain: GS77; resistant to cerulenin.
Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving system
5,10-methylenetetrahydrofolate reductase
Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
Probable amino-acid import ATP-binding protein YxeO
ATP phosphoribosyltransferase regulatory subunit
Aminoacyl-transfer RNA synthetases class-II family profile
Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom
Putative metal-sulfur cluster biosynthesis proteins YuaD
Lichenan-specific phosphotransferase enzyme IIA component
Tele-phosphohistidine intermediate; by HPr
Putative cytochrome bd menaquinol oxidase subunit I
Uncharacterized HTH-type transcriptional regulator YdzF
SPbeta prophage-derived uncharacterized protein YorX
Thymidylate synthase/dCMP hydroxymethylase
Type II restriction enzyme BsuMI component YdiR
Uncharacterized HTH-type transcriptional regulator YqaG
SPbeta prophage-derived uncharacterized protein YorT
SPbeta prophage-derived uncharacterized protein YotD
Putative S-adenosyl-L-methionine-dependent methyltransferase YktD
ABC transporter glutamine-binding protein GlnH
Ionotropic glutamate receptor C-terminal
Solute-binding protein family 3/N-terminal
Manganese transport system ATP-binding protein MntB
8000-fold reduction in catalytic activity.
Oligopeptide transport system permease protein OppC
4-fold reduction in activity. Abolished activity; when associated with A-162; A-163 and A-165.
4-fold reduction in activity. Abolished activity; when associated with A-162; A-163 and A-167.
4-fold reduction in activity. Abolished activity; when associated with A-162; A-165 and A-167.
4-fold reduction in activity. Abolished activity; when associated with A-163; A-165 and A-167.
Abolishes autophosphorylation and decreases spore production and biofilm formation. No phosphorylation of YezB.
Does not support germination in response to peptidoglycan.
Uncharacterized MscS family protein YkuT
Diacylglycerol glucosyltransferase N-terminal
Processive diacylglycerol beta-glucosyltransferase
Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC
SPbeta prophage-derived uncharacterized protein YorJ
Sporulation initiation phosphotransferase B
Sporulation initiation phosphotransferase B C-terminal
ATP-dependent helicase/deoxyribonuclease subunit B
Loss of iron-sulfur group binding, loss of DNA-binding.
Some loss of nuclease activity, helicase and DNA-binding are unaltered; when associated with A-1172 in AddA nearly complete loss of nuclease activity.
Important in orienting the substrate; probably interacts with magnesium or manganese
Probable undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase
Uncharacterized HTH-type transcriptional regulator YdfD
Glutamyl-tRNA(Gln) amidotransferase subunit C
Heptaprenyl diphosphate synthase component 2
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Uncharacterized MFS-type transporter YddS
Zinc-specific metallo-regulatory protein
SPbeta prophage-derived uncharacterized protein YorN
PTS system trehalose-specific EIIBC component
Phosphocysteine intermediate; for EIIB activity
Fe-S protein maturation auxiliary factor YitW
Uncharacterized N-acetyltransferase YoaP
Uncharacterized transcriptional regulatory protein YrkP
Probable ATP-dependent DNA helicase RecS
tRNA threonylcarbamoyladenosine dehydratase
Putative PTS system glucosamine-specific EIICBA component
Tele-phosphohistidine intermediate; for EIIA activity
Putative UDP-glucose 6-dehydrogenase YtcA
UDP-glucose/GDP-mannose dehydrogenase C-terminal
Aminodeoxychorismate synthase component 1
Anthranilate synthase component I N-terminal
Complete loss of aminodeoxychorismate synthase activity.
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Linearmycin resistance permease protein LnrN
Molybdopterin synthase sulfur carrier subunit
D1, associates with and fuses membranes, tethers lipsomes
No change in membrane fusion. Still binds GTP, no GTPase activity. No longer binds GTP, no GTPase activity; when associated with A-56. Double mutant causes better vesicle and content mixing than wild-type.
No change in membrane fusion. Still binds GTP, no GTPase activity. No longer binds GTP, no GTPase activity; when associated with A-625. Double mutant causes better vesicle and content mixing than wild-type.
Proton acceptor; for dehydratase activity 1
Proton acceptor; for dehydratase activity 2
Proton acceptor; for dehydratase activity 3
Proton donor; for dehydratase activity 1
Proton donor; for dehydratase activity 2
Proton donor; for dehydratase activity 3
Methylated-DNA--protein-cysteine methyltransferase, inducible
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
Methylguanine DNA methyltransferase ribonuclease-like
SPbeta prophage-derived UPF0714 protein YoqZ
SPbeta prophage-derived uncharacterized protein YolB
Bifunctional riboflavin kinase/FMN adenylyltransferase
Eliminates Spx binding. Does not enhance Spx proteolysis.
Putative metabolite transport protein YncC
Probable low-affinity inorganic phosphate transporter
Interaction with polymerase core subunit RpoC
Uncharacterized HTH-type transcriptional regulator YwoH
DNA-directed RNA polymerase subunit omega
Methyl-accepting chemotaxis protein McpA
No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-593.
No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-594.
No activity. No iron-sulfur cluster formed.
Performs first half of the reaction. No effect on iron-sulfur cluster formation.
SPbeta prophage-derived uncharacterized protein YosK
4-hydroxy-tetrahydrodipicolinate synthase
Protein-arginine kinase activator protein
Uncharacterized HTH-type transcriptional regulator YvzC
Probable siderophore transport system permease protein YfiZ
Uncharacterized MFS-type transporter YdeG
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
Loss of enzyme activity and N-terminal processing.
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
SkfA peptide export ATP-binding protein SkfE
SPbeta prophage-derived uncharacterized protein YoqO
Uncharacterized beta-barrel protein YwiB
Arabinogalactan endo-beta-1,4-galactanase
SPbeta prophage-derived aminoglycoside N(3')-acetyltransferase-like protein YokD
Constitutively active; causes constitutive activation of SigW.
Involved in sensing cell envelope stress
Uncharacterized ABC transporter ATP-binding protein YclH
2000-fold reduction in catalytic efficiency.
6300-fold reduction in catalytic efficiency.
About 2-fold decrease in substrate affinity and 30-fold reduction in catalytic activity.
About 2-fold decrease in substrate affinity and 70-fold reduction in catalytic activity.
Increases nucleophilicity of active site Cys
Putative HTH-type DNA-binding domain-containing acetyltransferase YbfA
Activator of Hsp90 ATPase homologue 1-like C-terminal
Dipeptide transport system permease protein DppC
Reversibly protonated during proton transport
In DnaBI; required for chromosome and plasmid replication.
In DnaBII; required only for chromosome replication.
Replication initiation and membrane attachment protein
Oligopeptide transport ATP-binding protein AppD
Uncharacterized MFS-type transporter YkuC
SPbeta prophage-derived membrane protein YosA
Dihydrofolate synthase/folylpolyglutamate synthase
Uncharacterized transcriptional regulatory protein YcbL
Uncharacterized HTH-type transcriptional regulator YdeP
Influences the redox potential of the prosthetic heme and FAD groups
Involved in heme-bound ligand stabilization and O-O bond activation
Probable UbiX-like flavin prenyltransferase
Uncharacterized N-acetyltransferase YesJ
Oligopeptide transport ATP-binding protein OppF
Putative phosphotransferase enzyme IIA component YpqE
Probable threonine/serine transporter YbxG
SPbeta prophage-derived uncharacterized protein YoqE
Probable ribosomal RNA small subunit methyltransferase B
RNA polymerase sigma factor 70 region 4 type 2
Spore germination protein-like protein YpzD
Retains its autophosphorylation activity, but cannot phosphorylate DegU.
Retains its autophosphorylation activity, but shows a decrease in DegU phosphorylation activity. Decreases the rate of dephosphorylation of DegU.
Shows increased phosphorylation, as well as increased autophosphorylation and phosphotransfer to DegU. Negatively affects competence development.
Signal transduction histidine-protein kinase/phosphatase DegS
CDP-diacylglycerol--serine O-phosphatidyltransferase
Polysaccharide biosynthesis protein C-terminal
Uncharacterized N-acetyltransferase YvbK
DNA-directed DNA polymerase family A palm
Membrane insertase YidC/Oxa/ALB C-terminal
Uncharacterized HTH-type transcriptional regulator YezC
Prophage-derived-like uncharacterized protein YozM
Uncharacterized HTH-type transcriptional regulator YkuM
Uncharacterized HTH-type transcriptional regulator YhdI
Uncharacterized HTH-type transcriptional regulator YwhA
SPbeta prophage-derived uncharacterized protein YorQ
Probable ABC transporter permease protein YqgI
Thiazolidine linkage to a ring-opened DNA abasic site
Uncharacterized MFS-type transporter YxlH
Small, acid-soluble spore protein gamma-type
Uncharacterized MFS-type transporter YxaM
Putative ribosomal RNA large subunit methyltransferase YwbD
O-(5'-phospho-DNA)-tyrosine intermediate
Cystathionine gamma-synthase/O-acetylhomoserine (thiol)-lyase
Essential for prephenate dehydratase activity
Teichuronic acid biosynthesis protein TuaB
Cytochrome C biogenesis protein transmembrane
Cytochrome c-type biogenesis protein CcdA
Uncharacterized HTH-type transcriptional regulator YvbU
Putative arsenical pump membrane protein YdfA
Accumulation of S-bacillithiolated form due to lack of this resolving cysteine residue. Debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) without the prior NaBH(4) reduction of this protein in vitro. Regenerates active OhrR with DNA-binding activity, but unable to activate the S-cysteinyl cysteine modified form of OhrR (OhrR-SSCys) in vitro. Debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB).
Loss of debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) by the NaBH(4)-reduced form of this protein in vitro. Loss of regeneration of active OhrR with DNA-binding activity in vitro. Loss of debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB).
Response regulator aspartate phosphatase B
Uncharacterized HTH-type transcriptional regulator YfiF
Methyl-accepting chemotaxis protein TlpC
Uncharacterized N-acetyltransferase YobR
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Uncharacterized HTH-type transcriptional regulator YulB
Helical; Signal-anchor for type II membrane protein
Glutathione/L-cysteine transport system ATP-binding/permease protein CydC
DNA-directed RNA polymerase subunit delta
Lysidine-tRNA(Ile) synthetase C-terminal
Putative enoyl-CoA hydratase/isomerase YhaR
Uncharacterized HTH-type transcriptional regulator FruR
SPbeta prophage-derived uncharacterized protein YorG
Uncharacterized HTH-type transcriptional regulator YoaU
Septum formation inhibitor MinC C-terminal
3-fold reduction in catalytic efficiency.
4-fold reduction in catalytic efficiency.
Did not get expressed in heterologous host.
Teichoic acid poly(glycerol phosphate) polymerase
Uncharacterized ABC transporter ATP-binding protein YvfR
DNA-binding transcriptional repressor ScoC
Uncharacterized RNA pseudouridine synthase YjbO
Enzyme activity changes to monoacylglycerol hydrolase. Marked decrease in temperature stability, decreased stability at pH 11 but increased stability at pH 5.
Uncharacterized MFS-type transporter YvkA
Cation-transporting P-type ATPase N-terminal
Probable nitrate reductase molybdenum cofactor assembly chaperone NarJ
Probable sodium/glutamine symporter GlnT
Probable transcriptional regulatory protein YeeI
O-(3'-phospho-DNA)-tyrosine intermediate
SPbeta prophage-derived probable integrase/recombinase YopP
Beta-ketoacyl-[acyl-carrier-protein] synthase III 1
Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal
Beta-ketoacyl-[acyl-carrier-protein] synthase III N-terminal
Schiff-base intermediate with KHG or pyruvate
Proton acceptor; for dehydratase activity
SPbeta prophage-derived uncharacterized protein YoqS
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Putative peptide biosynthesis protein YydG
Changes the metal specificity of the enzyme. Can insert Co(2+) into protoporphyrin IX at a higher rate than the wild-type enzyme, but loses the ability to insert Cu(2+) and Zn(2+).
DNA replication/recombination mediator RecO N-terminal
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
SPbeta prophage-derived uncharacterized protein YoqH
Uncharacterized HTH-type transcriptional regulator YugG
Large ribosomal subunit protein uL2 C-terminal
Uncharacterized HTH-type transcriptional regulator YdgG
Probable xanthine dehydrogenase subunit A
Cell envelope-related transcriptional attenuator
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagV
SPbeta prophage-derived uncharacterized protein YopF
L-cystine transport system permease protein TcyB
Homooligomerizes, no change in number of foci.
No longer homooligomerizes, poor aggregation, severe decrease in the number of foci.
No longer homooligomerizes, poor aggregation, severe decrease in the number of foci. Protein is dispersed in the cell membrane.
Oligopeptide transport system permease protein OppB
Phosphoribosylformylglycinamidine synthase subunit PurS
Manganese-dependent inorganic pyrophosphatase
PhoD-like phosphatase metallophosphatase
Uncharacterized MFS-type transporter YubD
Probable multidrug resistance protein YpnP
Transcription termination/antitermination protein NusG
Peptidase S9 prolyl oligopeptidase catalytic
Probable ABC transporter permease protein YesQ
Pyruvate dehydrogenase E1 component subunit alpha
Cleaves Holliday junctions, no interaction with RecA, greatly increased sensitivity to DNA-damaging agents.
Greatly increased sensitivity to DNA-damaging agents, chromosome segregation defects. Binds DNA and is able to cleave a Holliday junction.
Greatly increased sensitivity to DNA-damaging agents, chromosome segregation defects. Binds DNA but cannot cleave a Holliday junction.
Loss of Holliday junction resolution activity.
Reduces Holliday junction resolution activity 6-fold.
Flagellar basal body rod protein N-terminal
SPbeta prophage-derived uncharacterized protein YopX
Probable L-serine dehydratase, alpha chain
Probable spore germination protein GerPA
Unable to complement the cell division phenotype.
Uncharacterized N-acetyltransferase YjcF
Uncharacterized HTH-type transcriptional regulator YdfL
ABC transporter ATP-binding protein YtrE
SPbeta prophage-derived uncharacterized lipoprotein YonS
Uncharacterized ABC transporter ATP-binding protein YvrA
Able to complement for the deletion of this protein.
Loss of ATPase activity. Loss of ability to complement for the deletion of this protein.
Loss of ability to complement for the deletion of this protein.
Loss of ability to complement for the deletion of this protein. Loss of native structure.
No effect on susceptibility to DNA damage.
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
3-isopropylmalate dehydratase small subunit
Aconitase A/isopropylmalate dehydratase small subunit swivel
Methyl-accepting chemotaxis protein TlpB
SPbeta prophage-derived uncharacterized protein YonF
Petrobactin import system permease protein YclN
Sporulation inhibitor of replication protein SirA
Schiff-base intermediate with substrate; via pyruvic acid
SPbeta prophage-derived uncharacterized protein YopN
Succinate dehydrogenase cytochrome b558 subunit
Uncharacterized ABC transporter ATP-binding protein YfiC
Peptidase G2 IMC autoproteolytic cleavage
Has little or no effect on sporulation in wild-type SpoIIP cells. Restores sporulation efficiency of SpoIIPG-108 mutant almost to the level of the wild-type. Localization of SpoIIPG-108 mutant to the septum is about 78% that of wild-type SpoIIP. Fails to suppress the sporulation defect of mutants SpoIIPR-189 and R-278 as well as SpoIIP double mutant L-54 and P-114.
Partially restores the sporulation efficiency of SpoIIPG-108 mutant.
Uncharacterized HTH-type transcriptional regulator YraN
Prespore-specific transcriptional regulator RsfA
Methylated-DNA--protein-cysteine methyltransferase, constitutive
Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha
Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.
Putative HMP/thiamine permease protein YkoC
Sporulation initiation phosphotransferase F
Na+/H+ antiporter MnhB subunit-related protein
Uncharacterized ABC transporter permease YvrN
Uncharacterized HTH-type transcriptional regulator YtzE
Uncharacterized N-acetyltransferase YnaD
Response regulator aspartate phosphatase J
Shows wild-type phosphatase activity and sensitivity to PhrC inhibition.
Shows wild-type phosphatase activity but is insensitive to PhrC inhibition.
Uncharacterized HTH-type transcriptional regulator YwqM
Pyruvate phosphate dikinase AMP/ATP-binding
Fe(3+)-citrate import system permease protein YfmE
Putative aliphatic sulfonates transport permease protein SsuC
SPbeta prophage-derived uncharacterized protein YomV
Minor teichoic acid biosynthesis protein GgaB
Probable 4-aminobutyrate aminotransferase
Uncharacterized ABC transporter ATP-binding protein YwjA
Uncharacterized transcriptional regulatory protein YvfU
Abolishes lipid modification of the protein.
Decrease in PPIase activity. No effect on secretion of proteins and on growth.
Decreased secretion of proteins; no effect on growth.
Loss of PPIase function. No effect on secretion of proteins and on growth.
No effect on secretion of proteins and on growth.
SPbeta prophage-derived UPF0715 membrane protein YopD
Linearmycin resistance permease protein LnrM
Arginine transport ATP-binding protein ArtM
Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB
Uncharacterized N-acetyltransferase YjdG
Prepilin leader peptidase/N-methyltransferase
Prepilin type IV endopeptidase peptidase
High-affinity zinc uptake system ATP-binding protein ZnuC
Phosphomannose isomerase type I C-terminal
Phosphomannose isomerase type I catalytic
Putative mannose-6-phosphate isomerase YvyI
Oligosaccharides import ATP-binding protein MsmX
Impairs sporulation inhibition activity.
In spo0E11; a hyperactive phosphatase, it is no longer a substrate for FtsH.
Destabilization of structural stability and loss of functionality.
3-hydroxyacyl-CoA dehydrogenase C-terminal
3-hydroxyacyl-CoA dehydrogenase NAD binding
Probable 3-hydroxybutyryl-CoA dehydrogenase
FMN-dependent NADH:quinone oxidoreductase 1
Cytochrome b/b6 N-terminal region profile
Menaquinol:cytochrome c reductase cytochrome b subunit
Polygalacturonan/rhamnogalacturonan transport system permease protein YteP
In mut9; sporulation efficiency decreases by about 4 orders of magnitude.
Polysaccharide chain length determinant N-terminal
Probable capsular polysaccharide biosynthesis protein YwqC
Mg(2+)/citrate complex secondary transporter
Uncharacterized HTH-type transcriptional regulator YvdT
Uncharacterized transcriptional regulatory protein YxjL
Phosphate import ATP-binding protein PstB 2
Probable tartrate dehydrogenase/decarboxylase
Probable siderophore-binding lipoprotein YfiY
Uncharacterized transcriptional regulatory protein YkoG
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Dipeptide transport system permease protein DppB
PTS system fructose-specific EIIA component
Phosphorylation is only slightly increased.
Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase
Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation
Uncharacterized MFS-type transporter YceJ
Uncharacterized glycosyltransferase EpsJ
No longer confers resistance to virginiamycin M, still confers resistance to lincomycin and tiamycin.
No longer confers resistance to virginiamycin M.
Probably has no ATPase activity, does not confer lincomycin resistance, cells enter stationary phase at lower cell density, almost all protein is bound to ribosomes; when associated with Q-129 (called EQ2). Used for electron microscopy.
Probably has no ATPase activity, does not confer lincomycin resistance, cells enter stationary phase at lower cell density, almost all protein is bound to ribosomes; when associated with Q-432 (called EQ2). Used for electron microscopy.
Still confers resistance to virginiamycin M, lincomycin and tiamycin.
Destroys both polymerase and exonuclease activities.
Destroys exonuclease activity; decreases polymerase activity.
Polymerase/histidinol phosphatase N-terminal
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YcsE
Manganese/iron superoxide dismutase C-terminal
Manganese/iron superoxide dismutase N-terminal
N-(5'-phosphoribosyl)anthranilate isomerase
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
Binds DNA, but loss of arsenite-mediated regulation; when associated with S-33.
Binds DNA, but loss of arsenite-mediated regulation; when associated with S-35.
SPbeta prophage-derived uncharacterized protein YonV
Glycerol phosphate lipoteichoic acid synthase 2
Processed glycerol phosphate lipoteichoic acid synthase 2
Tryptophan synthase beta chain-like PALP
Uncharacterized MFS-type transporter YuxJ
Putative maltodextrin utilization protein YvdJ
Putative 23S rRNA (guanine-N(1)-)-methyltransferase YxjB
Does not abrogate the development of aerial mycelium in S.griseus.
Probable quorum-quenching lactonase YtnP
Uncharacterized ABC transporter ATP-binding protein YybJ
Uncharacterized SPbeta prophage-derived protein YotG
Glutathione/L-cysteine transport system ATP-binding/permease protein CydD
Probable glutamine ABC transporter permease protein GlnP
Uncharacterized MFS-type transporter YfhI
Uncharacterized glycosyltransferase YkoN
Uncharacterized HTH-type transcriptional regulator YobQ
Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose
Spore coat polysaccharide biosynthesis protein SpsL
SPbeta prophage-derived uncharacterized protein YoqA
Transcriptional regulator SgrR N-terminal HTH
Putative sporulation-specific glycosylase YdhD
Putative amidase domain-containing protein
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabL
17-fold increased production of c-di-AMP in E.coli.
20-fold increased production of c-di-AMP in E.coli, forms only homodimers.
Suppresses a triple DAC mutant, 10-fold increased production of c-di-AMP in E.coli, forms hexamers.
Suppresses a triple DAC mutant, 12-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 20-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 4-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 90-fold increased production of c-di-AMP in E.coli.
Heptaprenyl diphosphate synthase component 1
Increases the stability of phosphorylated form.
Lack of phosphorylation. Decreases degradative enzyme production, but allows full expression of late competence genes.
Prevents both swarming motility and pellicle formation.
Uncharacterized N-acetyltransferase YhfO
Mandelate racemase/muconate lactonizing enzyme C-terminal
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Methyl-accepting chemotaxis protein McpC
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.
Loss of cysteine desulfurase activity, still binds SufU and Cys.
Sporulation integral membrane protein YlbJ
DNA-directed RNA polymerase subunit beta'
In streptolydigan resistant alleles stl6/stl445.
Flagellar motor switch protein FliN-like C-terminal
SPbeta prophage-derived uncharacterized protein YonI
Melibiose/raffinose/stachyose import permease protein MelC
Helix-hairpin-helix DNA-binding motif class 1
SPbeta prophage-derived uncharacterized protein YopQ
Uncharacterized ABC transporter substrate-binding lipoprotein YvrC
Probable spore germination protein GerPD
PTS system oligo-beta-mannoside-specific EIIB component
Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
PTS system mannose-specific EIIBCA component
Glycosyltransferase subfamily 4-like N-terminal
Putative teichuronic acid biosynthesis glycosyltransferase TuaC
Uncharacterized metal-dependent hydrolase YabD
Arginine-binding extracellular protein ArtP
Energy-coupling factor transporter ATP-binding protein EcfA2
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
SPbeta prophage-derived uncharacterized protein YosI
Cyclic pyranopterin monophosphate synthase
Molybdopterin cofactor biosynthesis C (MoaC)
SPbeta prophage-derived uncharacterized protein YomY
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Transcription elongation factor GreA/GreB C-terminal
Transcription elongation factor GreA/GreB N-terminal
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
SPbeta prophage-derived uncharacterized protein YopG
RND efflux pump membrane fusion protein barrel-sandwich
PTS system beta-glucoside-specific EIIBCA component
Asparagine synthetase [glutamine-hydrolyzing] 3
Important for beta-aspartyl-AMP intermediate formation
Probable queuosine precursor transporter
Large ribosomal subunit protein uL5 C-terminal
Large ribosomal subunit protein uL5 N-terminal
Uncharacterized HTH-type transcriptional regulator YybR
Tetrapyrrole biosynthesis uroporphyrinogen III synthase
Does not affect binding to FlhF, but abolishes its activation.
Type III secretion system flagellar brake protein YcgR PilZN
Probable peptidoglycan glycosyltransferase FtsW
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Cobalamin-independent methionine synthase MetE C-terminal/archaeal
Cobalamin-independent methionine synthase MetE N-terminal
Impaired chemotaxis. No binding to CheD. Almost no enhancement of CheC activity by CheD.
SPbeta prophage-derived uncharacterized protein YonT
Spore germination protein-like protein YueG
ATP-dependent dethiobiotin synthetase BioD
Uncharacterized N-acetyltransferase YitI
KinB-signaling pathway activation protein
Catabolic NAD-specific glutamate dehydrogenase RocG
Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Increase of thermostability 8 degrees Celsius higher than that of the wild-type.
Reduces the affinity for glutamate and ammonium.
Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
SPbeta prophage-derived uncharacterized protein YorY
Chromosomal replication initiator protein DnaA
30-fold decrease in activity but no decrease in the overall phosphorylation level.
Probable C4-dicarboxylate response regulator DctR
Uncharacterized HTH-type transcriptional regulator YxjO
Uncharacterized HTH-type transcriptional regulator YhjH
SPbeta prophage-derived uncharacterized protein YomO
Putative ATP-dependent DNA helicase YjcD
Glutamine transport ATP-binding protein GlnQ
Sulfite reductase [NADPH] flavoprotein alpha-component
Strongly reduces catalytic efficiency and enzyme activity. Slightly increases substrate affinity.
Strongly reduces catalytic efficiency while increasing substrate affinity.
Electron transfer flavoprotein alpha/beta-subunit N-terminal
Electron transfer flavoprotein subunit beta
Assimilatory nitrate reductase catalytic subunit
No activity, but can, as the wild-type, form covalent adduct with ribose-5-phosphate.
No activity, does not form covalent adduct with ribose-5-phosphate or ribulose 5-phosphate.
No activity, does not form covalent adduct with ribose-5-phosphate.
Pyridoxal 5'-phosphate synthase subunit PdxS
Results in 20-fold reduction of activity.
Schiff-base intermediate with D-ribose 5-phosphate
Glycine betaine transport system permease protein OpuAB
Peptidase S33 tripeptidyl aminopeptidase-like C-terminal
Probable galactarate dehydratase (L-threo-forming)
Extracellular matrix regulatory protein A
1-deoxy-D-xylulose 5-phosphate reductoisomerase
1-deoxy-D-xylulose 5-phosphate reductoisomerase C-terminal
1-deoxy-D-xylulose 5-phosphate reductoisomerase N-terminal
SPbeta prophage-derived uncharacterized protein YonG
Putative peptidoglycan O-acetyltransferase YrhL
Spore coat polysaccharide biosynthesis protein SpsB
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B
SPbeta prophage-derived uncharacterized protein YomF
Antilisterial bacteriocin subtilosin biosynthesis protein AlbE
Putative ABC transporter ATP-binding protein YjkB
tRNA pseudouridylate synthase B C-terminal
Uncharacterized AAA domain-containing protein YrvN
Putative sugar dehydratase/epimerase YfnG
FMN-dependent NADH:quinone oxidoreductase 2
Complete loss of ability to inhibit KinA autophosphorylation.
Partial loss of ability to inhibit KinA autophosphorylation.
Pseudouridine synthase I TruA alpha/beta
Fe(3+)-citrate import ATP-binding protein YfmF
Polysaccharide biosynthesis protein CapD-like
Probable polysaccharide biosynthesis protein EpsC
SPbeta prophage-derived uncharacterized protein YotE
Inner membrane protein YgaP-like transmembrane
Inhibits autoproteolysis of protein and spore formation.
Reduced binding to PDZ domain and sporulation deficient spores.
Leads to copper binding and enzyme activity; when associated with H-104.
Leads to copper binding and enzyme activity; when associated with H-88.
Superoxide dismutase copper/zinc binding
Putative aromatic acid exporter C-terminal
Loss of phosphorylation. Interacts strongly with RsbW.
Probable spore germination protein GerPB
Acyl-ester intermediate; for transpeptidase activity
Proton donor; for transglycosylase activity
General stress protein FMN-binding split barrel
Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal
Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
SPbeta prophage-derived uncharacterized protein YonB
Arabinooligosaccharides transport system permease protein AraQ
Negative impact in the growth rate in the presence of arabinotriose.
Phosphatidic acid phosphatase type 2/haloperoxidase
Sporulation transcription regulator WhiA N-terminal
N-acetyl-S-(2-succino)cysteine monooxygenase
Shows 0.9% of wild-type catalytic activity.
Shows 10% of wild-type catalytic activity.
Shows 36% of wild-type catalytic activity.
Probable polyketide biosynthesis zinc-dependent hydrolase PksB
Full repressor activity, but no modulation by peroxide.
May have an important role in the acetylation of the polyamine
Spermidine/spermine N(1)-acetyltransferase
SPbeta prophage-derived uncharacterized protein YopJ
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
Succinate dehydrogenase flavoprotein subunit
Proline-responsive transcriptional activator PutR
Germination-specific N-acetylmuramoyl-L-alanine amidase
Glycoside hydrolase family 65 C-terminal
Glycoside hydrolase family 65 N-terminal
Glycoside hydrolase family 65 central catalytic
Plays an important role in maintaining the position of the catalytic nucleophile
Loss of catalytic activity. Inability to form the acyl-enzyme intermediate.
SPbeta prophage-derived uncharacterized protein YosX
Uncharacterized N-acetyltransferase YlbP
Purine nucleoside phosphorylase DeoD-type
Putative HTH-type transcriptional regulator YvaV
SPbeta prophage-derived uncharacterized protein YomR
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
2-oxoisovalerate dehydrogenase subunit alpha
Putative polysaccharide deacetylase YheN
Binds c-di-AMP, but interaction with RelA is weakened and not affected by c-di-AMP; when associated with G-25.
Binds c-di-AMP, but interaction with RelA is weakened and not affected by c-di-AMP; when associated with M-132.
Binds c-di-AMP. Abolishes the ability to bind RelA. Is still able to bind PYC, but cannot activate it.
Almost no effect on catalytic efficiency.
Hydrolyzes ATP, even in the absence of L-Ala, and the structure appears to show a cavity in the N-terminal substrate-binding pocket. Also alters the substrate specificity and activity depending on the size and shape of substituted amino acids.
Plays a key role in restricting the N-terminal substrate specificity to small amino acids such as L-Ala
Great decrease in binding affinity for mRNA.
Increased ability to bind to hut mRNA in the presence of histidine.
Slight decrease in binding affinity for mRNA.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
L-cystine transport system permease protein TcyL
Putative aliphatic sulfonates-binding protein
In JH17041; thermosensitive; decrease in dimerization and DNA-binding.
SPbeta prophage-derived uncharacterized protein YonX
Translation initiation factor 3 C-terminal
Translation initiation factor 3 N-terminal
Ribosomal RNA small subunit methyltransferase I
Uncharacterized isochorismatase family protein PncA
Sec-independent protein translocase protein TatCy
Glycerol uptake operon antiterminator regulatory protein
SPbeta prophage-derived uncharacterized protein YosL
Protein-glutamine gamma-glutamyltransferase
Uncharacterized MFS-type transporter YhjO
6-phosphogluconate dehydrogenase C-terminal
Putative 6-phosphogluconate dehydrogenase YqeC
tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding
Melibiose/raffinose/stachyose import permease protein MelD
SPbeta prophage-derived uncharacterized protein YorR
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Probable NADH-dependent butanol dehydrogenase 1
Molybdenum cofactor biosynthesis protein B
Large ribosomal subunit protein uL15/eL18
Putative penicillin-binding protein PbpX
6-carboxy-5,6,7,8-tetrahydropterin synthase
Loss of phosphodiesterase activity, does not confer antibiotic sensitivity when overexpressed.
PAS-like domain, required for heme-binding
Uncharacterized ABC transporter ATP-binding protein YlmA
SPbeta prophage-derived uncharacterized transglycosylase YomI
Asparagine synthetase [glutamine-hydrolyzing] 2
Uncharacterized HTH-type transcriptional regulator YvnA
SPbeta prophage-derived uncharacterized protein YonJ
Uncharacterized MFS-type transporter YvmA
Type II restriction enzyme BsuMI component YdjA
Uncharacterized HTH-type transcriptional regulator YybA
SPbeta prophage-derived uncharacterized protein YosB
Probable spore germination protein GerPE
Cannot restore a sporulation-proficient phenotype in a phrA deletion mutant.
Restores a sporulation-proficient phenotype in a phrA deletion mutant.
Probable serine/threonine-protein kinase YabT
tRNA-specific 2-thiouridylase MnmA-like C-terminal
tRNA-specific 2-thiouridylase MnmA-like central
Formamidopyrimidine-DNA glycosylase catalytic
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Bacitracin export ATP-binding protein BceA
Uncharacterized transmembrane protein YshB
SPbeta prophage-derived uncharacterized protein YorH
Uncharacterized MFS-type transporter YfkL
Flagellar basal-body/hook protein C-terminal
Flagellar hook-basal body complex protein FlhO
RlpA-like protein double-psi beta-barrel
Energy-coupling factor transporter ATP-binding protein EcfA1
Sporulation thiol-disulfide oxidoreductase A
SPbeta prophage-derived glycosyltransferase SunS
Uncharacterized HTH-type transcriptional regulator YhbI
YbaK/aminoacyl-tRNA synthetase-associated
SPbeta prophage-derived uncharacterized membrane protein YotH
Wild-type growth rate, this enzyme probably has no synthase activity but can still degrade pppGpp to ppGpp.
Uncharacterized HTH-type transcriptional regulator YwaE
SPbeta prophage-derived uncharacterized protein YomZ
Choloylglycine hydrolase/NAAA C-terminal
Spore coat polysaccharide biosynthesis protein SpsE
Uncharacterized sodium-dependent transporter YhdH
In grl-15; loss of glucose repression, deficiency in glucose transport and absence of ptsG expression.
In grl-30; loss of glucose repression, deficiency in glucose transport and absence of ptsG expression.
Teichoic acid translocation permease protein TagG
Probable multidrug resistance protein YoeA
SPbeta prophage-derived uncharacterized protein YokH
Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding
Dipicolinate synthase subunit A N-terminal
SPbeta prophage-derived uncharacterized protein YomP
Probable inorganic carbon transporter subunit DabA
Uncharacterized HTH-type transcriptional regulator YvkB
Complete loss of ATPase activity; mutant proteins trap ATP in a vanadate-independent manner whereas the wild-type protein traps ADP.
Multidrug resistance ABC transporter ATP-binding/permease protein BmrA
Retains 2% ATPase activity; unable to transport Hoechst 33342. Traps ADP in a beryllium fluoride-dependent manner, confirming ATPase activity. Probably unable to undergo NBD dimerization.
Guanosine ABC transporter permease protein NupQ
SPbeta prophage-derived uncharacterized N-acetyltransferase YokL
Putative HTH-type transcriptional regulator YwgB
D-alanyl-D-alanine carboxypeptidase DacB
Peptidase S11 D-Ala-D-Ala carboxypeptidase A C-terminal
serine-type D-Ala-D-Ala carboxypeptidase
SPbeta prophage-derived uncharacterized protein YosV
Sec translocon accessory complex subunit YrbF
Putative NADP-dependent oxidoreductase YfmJ
ABC-type glycine betaine transport system substrate-binding
L-cystine import ATP-binding protein TcyC
Uncharacterized MFS-type transporter YitZ
ABC transporter ATP-binding protein YxdL
Na+-translocating membrane potential-generating system MpsC
Putative aspartate aminotransferase YhdR
Peptidoglycan beta-N-acetylmuramidase NamZ
Putative polyketide biosynthesis enoyl-CoA isomerase PksI
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Uncharacterized HTH-type transcriptional regulator YpoP
Probable membrane transporter protein YtnM
D-alanine--D-alanyl carrier protein ligase
23S rRNA (uracil-C(5))-methyltransferase RlmCD
Sigma 54 modulation/S30EA ribosomal protein C-terminal
20-fold reduction in sporulation efficiency. Largely enriched at the forespore periphery, although some localization to the cytosol. Binding of ATP reduced over 23-fold. Leads to diminished ATP hydrolysis.
500-fold decrease in sporulation efficiency. 5-log decrease in sporulation efficiency; no ATPase activity, but no change in ATP-binding ability; unable to polymerize and fails to promote coat assembly; when associated with A-71.
Altered cortex and coat formation, affects localization to the forespore.
Altered cortex and coat formation, affects localization to the forespore; when associated with P-400 and R-457.
Altered cortex and coat formation, affects localization to the forespore; when associated with P-400 and V-418.
Altered cortex and coat formation, affects localization to the forespore; when associated with R-457 and V-418.
Altered cortex and coat formation. Possesses swirls of coat in the mother cell cytoplasm and does not possess a cortex.
Altered cortex and coat formation; when associated with L-69.
Altered cortex and coat formation; when associated with S-230.
Altered cortex and coat formation; when associated with V-367 and R-383.
Completely abolished sporulation efficiency. No ATPase activity, but no change in ATP-binding ability. Unable to polymerize and fails to promote coat assembly;.
Defective in germination; when associated with A-210 and R-383. Altered cortex and coat formation; when associated with M-210 and R-383.
Defective in germination; when associated with A-210 and V-367. Altered cortex and coat formation; when associated with M-210 and R-383.
Defective in germination; when associated with N-283.
Defective in germination; when associated with P-59.
Defective in germination; when associated with S-98.
Defective in germination; when associated with V-367 and R-383.
Does not abrogate sporulation. 5-log decrease in sporulation efficiency; no ATPase activity, but no change in ATP-binding ability; unable to polymerize and fails to promote coat assembly; when associated with A-70.
Mislocalized to the mother cell cytosol. Misassembles the spore coat. Mislocalizes CotE but not SpoVM. Does not multimerize. Almost complete block in spore formation.
Spores form largely normal coats and possess at least some cortex, but cannot remain dehydrated after mother cell lysis. Abnormal synthesis and an insufficient level of spore peptidoglycan, which contains amidated diaminopimelic acid, which is normally found only in vegetative cell peptidoglycan. Defective in germination; when associated with P-256.
Sporulation stage IV protein A C-terminal
Suppressor mutation that reverses the mislocalization and sporulation defect of the A-6 mutation in SpoVM. Causes little impairment of sporulation.
Walker A motif; involved in ATP-binding and hydrolysis
Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit beta
Phosphoribosylaminoimidazole-succinocarboxamide synthase
1.5- and 2-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
5- and 2-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
5.5- and 3-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
6- and 3-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
SPbeta prophage-derived uncharacterized protein YosJ
2',3'-cyclic-nucleotide 2'-phosphodiesterase
Abolishes phosphodiesterase activity. Overexpresses flagellin and forms smooth colonies. Does not affect stability of the protein.
Increases cAMP levels. Displays aberrant colony morphologies.
Probable transcriptional regulatory protein YrbC
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
Glycine betaine/carnitine/choline-binding protein OpuCC
Uncharacterized N-acetyltransferase YdfB
2-iminobutanoate/2-iminopropanoate deaminase
Important for catalytic activity at high pH
Iron(3+)-hydroxamate-binding protein FhuD
No longer binds tRNA(Ala) anticodon (UGC), decreased accumulation of a stalled reporter protein.
No longer binds tRNA(Ala) anticodon (UGC), has minor Ala tailing ability, decreased accumulation of a stalled reporter protein.
No longer binds tRNA(Ala) anticodon (UGC), loss of Ala tailing ability, decreased accumulation of a stalled reporter protein.
Concentrative nucleoside transporter C-terminal
Concentrative nucleoside transporter N-terminal
Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]
Quinolinate phosphoribosyl transferase C-terminal
Quinolinate phosphoribosyl transferase N-terminal
Decrease in phosphatase activity in vitro. Displays reduced activity in vivo.
Displays drastically reduced activity in vivo.
Loss of phosphatase activity in vitro. Displays reduced activity in vivo.
No change in phosphatase activity in vitro.
Response regulator aspartate phosphatase H
N-acetylmuramic acid 6-phosphate etherase
Aerobic glycerol-3-phosphate dehydrogenase
Alpha-glycerophosphate oxidase C-terminal
Uncharacterized ABC transporter extracellular-binding protein YurO
SPbeta prophage-derived uncharacterized membrane protein YosE
ATP-dependent Clp protease ATP-binding subunit ClpE
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
SPbeta prophage-derived uncharacterized protein YotK
Formate-dependent phosphoribosylglycinamide formyltransferase
Glucosamine/galactosamine-6-phosphate isomerase
Oxygen-independent coproporphyrinogen-III oxidase-like protein HemZ
Uncharacterized amino acid permease YecA
Uncharacterized HTH-type transcriptional regulator YwcC
Nitrite/Sulfite reductase ferredoxin-like
Sulfite reductase [NADPH] hemoprotein beta-component
Uncharacterized ABC transporter ATP-binding protein YfiB
Spore coat polysaccharide biosynthesis protein SpsC
Probable dual-specificity RNA methyltransferase RlmN
Completely abolishes the tyrosine-phosphatase activity.
Low molecular weight protein-tyrosine-phosphatase YfkJ
NADH-rubredoxin oxidoreductase C-terminal
S-(2-succino)cysteine N-acetyltransferase
Probable 2-phosphosulfolactate phosphatase
Putative oligopeptide transport ATP-binding protein YkfD
Sporulation stage II protein D amidase enhancer LytB N-terminal
Molybdopterin synthase catalytic subunit
Bifunctional transcriptional activator/DNA repair enzyme AdaA
Nucleophile; methyl group acceptor from methylphosphotriester
Uncharacterized MFS-type transporter YttB
Putative type II methyltransferase M.BsuMIIP
Putative polysaccharide deacetylase YxkH
Uncharacterized amino acid permease YvbW
Probable 4-hydroxyphenylacetate 3-monooxygenase
SPbeta prophage-derived uncharacterized protein YolC
Transcription antitermination protein NusB
PTS system fructose-specific EIIABC component
scyllo-inositol 2-dehydrogenase (NADP(+)) IolW
Linearmycin resistance ATP-binding protein LnrL
Iron(3+)-hydroxamate-binding protein YxeB
Loss of ability to regulate the pyr operon and to bind to RNA; no effect on UPRTase activity.
Loss of ability to regulate the pyr operon; highly reduced affinity for RNA; no effect on UPRTase activity.
Loss of ability to regulate the pyr operon; no effect on UPRTase activity.
No effect on ability to regulate the pyr operon only in presence of excess pyrimidines; reduced affinity for RNA; no effect on UPRTase activity.
No effect on ability to regulate the pyr operon; no effect on uprtase activity.
Reduced ability to regulate the pyr operon, and loss of ability to bind to RNA; no effect on UPRTase activity.
Reduced ability to regulate the pyr operon; decreased UPRTase activity.
Reduced ability to regulate the pyr operon; reduced affinity for RNA; loss of UPRTase activity.
Probable HTH-type transcriptional regulator YttP
Polyketide biosynthesis malonyl-ACP decarboxylase PksF
Phosphoenolpyruvate-protein phosphotransferase
Phosphotransferase system enzyme I N-terminal
HTH-type transcriptional regulatory protein GabR
Manganese transport system membrane protein MntC
Probable guanidinium efflux system subunit GdnD
Uncharacterized HTH-type transcriptional regulator YdeF
Probable protein-export membrane protein SecG
Uncharacterized HTH-type transcriptional regulator YfiV
Probable ATP-dependent DNA helicase RecQ
ABC transporter ATP-binding protein YtrB
Putative pyruvate, phosphate dikinase regulatory protein
Putative SPbeta prophage-derived single-strand DNA-specific exonuclease YorK
Signal transduction histidine kinase subgroup 3 dimerisation and phosphoacceptor
SPbeta prophage-derived uncharacterized protein YokE
Uncharacterized HTH-type transcriptional regulator YqaE
Prevents inactivation of CtsR at high temperature.
Teichuronic acid biosynthesis protein TuaF
3- to 5-fold weaker affinity for PDZ ligands and reduced proteolytic activity against pre-processed SpoIVFA substrate. Less than residual protease activity; when associated with Y-118.
Carboxy-terminal processing protease CtpB
Cleavage; by SpoIVB, and cleavage; by autolysis
Constitutively active protease with higher activity than wild-type protease and total loss of substrate specificity.
Crucial for substrate binding and protease activation
Loss of peptide binding to the PDZ domain, but still has residual protease activity. Less than residual protease activity; when associated with A/F-168.
No cleavage by SpoIVB. No effect on pro-sigma-K processing.
Uncharacterized HTH-type transcriptional regulator YxaF
S-adenosylmethionine synthetase C-terminal
S-adenosylmethionine synthetase N-terminal
Levansucrase and sucrase synthesis operon antiterminator
SPbeta prophage-derived uncharacterized protein YonC
Cleaves SAM, but does not form thioether bond, contains 52% iron of wild-type.
No longer cleaves SAM, contains 55% iron of wild-type.
Sporulation killing factor maturation protein SkfB
SPbeta prophage-derived uncharacterized protein YoqP
Malonyl CoA-acyl carrier protein transacylase
Nucleophile; cysteine thiosulfonate intermediate
Phosphoadenosine phosphosulphate reductase
1-acyl-sn-glycerol-3-phosphate acyltransferase
N5-carboxyaminoimidazole ribonucleotide mutase
Is severely impaired in adaptation to the addition of asparagine. Shows near normal counterclockwise bias in the absence of attractant. Is not phosphorylated in the presence of CheA-P.
Uroporphyrinogen-III C-methyltransferase
Putative UTP--glucose-1-phosphate uridylyltransferase
UTP--glucose-1-phosphate uridylyltransferase
Uncharacterized N-acetyltransferase YdgE
Uncharacterized HTH-type transcriptional regulator YhgD
Putative 3-methyladenine DNA glycosylase
Bifunctional oligoribonuclease and PAP phosphatase NrnA
Choline transport ATP-binding protein OpuBA
Uncharacterized HTH-type transcriptional regulator YerO
Aspartate/ornithine carbamoyltransferase Asp/Orn-binding
Aspartate/ornithine carbamoyltransferase carbamoyl-P binding
PTS system sucrose-specific EIIBC component
SPbeta prophage-derived uncharacterized protein YorA
3-isopropylmalate dehydratase large subunit
Uncharacterized NAD(P)H oxidoreductase YrkL
Putative serine/threonine-protein kinase YrzF
Binds only one manganese ion, in a pseudo-hexacoordinate geometry.
Retains selectivity for activation by Mn(2+) and Cd(2+) over Co(2+) and Fe(2+). Can bind Mn(2+) in the C site, despite alteration to the A site, and adopt active DNA-binding conformations. Non-cognate ions fail to generate fully active complexes.
L-cystine transport system permease protein TcyM
Cysteine sulfenic acid (-SOH) intermediate
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Ribosomal RNA adenine methylase transferase N-terminal
Bifunctional purine biosynthesis protein PurH
SPbeta prophage-derived uncharacterized protein YosC
Uncharacterized HTH-type transcriptional regulator YdhC
L-threonine dehydratase biosynthetic IlvA
Abolishes ATP-binding and DNA translocation.
In SpoIIIE-73-11; defects in DNA translocation. Can complete engulfment and membrane fusion.
Flagellar hook-basal body complex protein FliE
Probable metabolite transport protein CsbC
Heme-copper oxidase subunit III family profile
Ribosomal RNA small subunit methyltransferase E
Ribosomal RNA small subunit methyltransferase E PUA-like
Ribosomal RNA small subunit methyltransferase E methyltransferase
Probable murein peptide carboxypeptidase
Oligopeptide transport system permease protein AppC
Multidrug-efflux transporter 2 regulator
Phosphate import ATP-binding protein PstB 1
DNA mismatch repair proteins mutS family
Complete loss of growth without mevalonate.
Does not activate SufS; dominant negative to wild-type protein, interacts with SufS. Binds about 40% Zn(2+).
Does not interact with SufS, does not activate SufS; no effect in presence of wild-type protein. Binds about 15% Zn(2+).
Does not interact with SufS, does not activate SufS; no effect in presence of wild-type protein. Binds about 45% Zn(2+).
Increases stability of the bound Fe-S cluster. Binds SufS, binds about 35% Zn(2+).
NIF system FeS cluster assembly NifU N-terminal
Probable galactarate/D-glucarate transporter GudP
Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes.
Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC.
Uncharacterized HTH-type transcriptional regulator YdeS
Succinate--CoA ligase [ADP-forming] subunit alpha
Molybdopterin-synthase adenylyltransferase
Glycine cleavage T-protein C-terminal barrel
Putative transcriptional regulator protein YobU
Probable amino-acid ABC transporter permease protein YckA
Probable polyketide biosynthesis enoyl-CoA hydratase PksH
No effect on DNA binding. Results in a weak constitutive ManR activity.
No reduction in DNA-binding activity can be observed in the presence of EIIBA-Man. Leads to a nearly constitutive ManR activity.
Putative multidrug export ATP-binding/permease protein YgaD
Cannot induce expression of trxA and trxB. Does not affect stability.
Confers defects in Spx-activated transcription but not in Spx-dependent repression.
Confers defects in Spx-activated transcription but not in Spx-dependent repression. Does not affect binding to SpxH/YjbH.
Decreases activity. 50% decrease in binding to SpxH/YjbH. Enhanced proteolysis by ClpXP in the absence of SpxH/YjbH.
Decreases activity. 80% decrease in binding to SpxH/YjbH. Enhanced proteolysis by ClpXP in the absence of SpxH/YjbH.
Decreases activity. 90% decrease in binding to SpxH/YjbH.
Retains activity. Resistant to degradation by ClpXP.
Uncharacterized ABC transporter permease YknZ
Probable membrane transporter protein YrkJ
Affects negative regulation of the antitermination activity.
Affects negative regulation of the antiterminator activity. No effect on phosphorylation by HPr. Abolishes phosphorylation by both HPr and EII-Glc; when associated with D-211.
Affects negative regulation of the antiterminator activity; when associated with S-102.
Affects negative regulation of the antiterminator activity; when associated with T-103.
Drastically reduced antiterminator activity.
Drastically reduced antiterminator activity; when associated with N-38.
Drastically reduced antiterminator activity; when associated with R-9.
Loss of antiterminator activity; when associated with L-5.
Loss of antiterminator activity; when associated with P-19.
Loss of antiterminator activity; when associated with S-5.
Loss of antiterminator activity; when associated with T-29.
No effect on negative regulation of the antiterminator activity.
Strongly reduced phosphorylation by HPr. Abolishes phosphorylation by both HPr and EII-Glc; when associated with A-104 or D-163.
Probable xanthine dehydrogenase subunit C
SPbeta prophage-derived ribonucleoside-diphosphate reductase subunit beta
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Menaquinone biosynthesis protein MenD middle
Gly-cisPro motif, important for rejection of L-amino acids
Uncharacterized amino acid permease YbgF
Uncharacterized MFS-type transporter YfiS
Beta-xylosidase C-terminal Concanavalin A-like
Peptidoglycan-N-acetylmuramic acid deacetylase PdaC
Destabilizes MgsR approximately by half. Almost loss of activity.
Destabilizes MgsR approximately by half. No change in activity.
Destabilizes MgsR approximately by half. Strong decrease in activity.
Loss of activity. Strongly diminishes expression of mgsR and of the MgsR regulon.
Slight increase in MgsR stability. Strong decrease in activity.
Strong decrease in MgsR stability. Almost loss of activity.
Strong decrease in MgsR stability. Strong decrease in activity.
Teichoic acids export ATP-binding protein TagH
Loss of (p)ppGpp synthesis, protein still forms tetramers.
No stimulation by (p)ppGpp, protein still forms tetramers.
Uncharacterized metallophosphoesterase YhaO
Uncharacterized glycosyltransferase YkcC
Electron transfer flavoprotein subunit alpha
Ribosomal RNA small subunit methyltransferase A
Uncharacterized MFS-type transporter YusP
ATP-dependent Clp protease proteolytic subunit
Has peptidase but not protease activity, activated by ADEP antibiotics.
Has peptidase but not protease activity, partially activated by ADEP antibiotics.
Has peptidase but not protease activity.
Protein no longer oligomerizes, loss of protease activity, activated by ADEP antibiotics.
Protein no longer oligomerizes, loss of protease activity.
Antilisterial bacteriocin subtilosin biosynthesis protein AlbD
Probable ABC transporter permease protein YurN
SPbeta prophage-derived uncharacterized protein YosF
Causes a severe defect in cell morphology and in chromosome segregation. Can still form filamentous structures, but these are rigid rather than dynamic.
Uncharacterized HTH-type transcriptional regulator YcbG
N-acetyl-alpha-D-glucosaminyl L-malate deacetylase 1
Uncharacterized HTH-type transcriptional regulator YdeC
Choline transport system permease protein OpuBD
Putative glycerophosphodiester phosphodiesterase YhdW
SPbeta prophage-derived stress response protein SCP1
No effect on protease activity in vitro.
Uncharacterized HTH-type transcriptional regulator YdgJ
Uncharacterized HTH-type transcriptional regulator YazB
Cytochrome oxidase subunit II copper A binding
Cytochrome oxidase subunit II transmembrane region profile
50% decrease in CSF production. 97% reduction in cleavage of pro-CSF by subtilisin and 42% reduction in cleavage by Vpr.
65% decrease in CSF production. 47% reduction in cleavage of pro-CSF by subtilisin and 79% reduction in cleavage by Vpr.
95% decrease in CSF production. 98% reduction in cleavage of pro-CSF by subtilisin and 90% reduction in cleavage by Vpr.
Competence and sporulation stimulating factor
Decreases transcription of the ComA-controlled gene rapA.
Does not stimulate expression of the srfA operon. Loss of inhibitory activity. Strong decrease in sporulation stimulation.
Does not stimulate expression of the srfA operon. No change in inhibitory activity and in sporulation stimulation.
Does not stimulate expression of the srfA operon. No change in inhibitory activity. Strong decrease in sporulation stimulation. Loss of activity toward RapB. Does not acquire any ability to inhibit RapA.
Does not stimulate expression of the srfA operon. Reduced inhibitory activity. Strong decrease in sporulation stimulation.
Increases activity toward RapB. Does not acquire any ability to inhibit RapA.
Loss of activity toward RapB. Does not acquire any ability to inhibit RapA.
Slightly increases transcription of the ComA-controlled gene rapA. No change in CSF levels.
Sulfur carrier protein ThiS adenylyltransferase
1,4-dihydroxy-2-naphthoate octaprenyltransferase
Gamma-D-glutamyl-L-lysine dipeptidyl-peptidase
Large ribosomal subunit protein bL25 L25
Large ribosomal subunit protein bL25 beta
SPbeta prophage-derived uncharacterized protein YoqT
Uncharacterized EAL-domain containing protein YkuI
SUF system FeS cluster assembly SufBD N-terminal
SPbeta prophage-derived uncharacterized protein YorV
N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase
Putative peptide zinc metalloprotease protein YydH
Lichenan-specific phosphotransferase enzyme IIB component
Glycine betaine/carnitine/choline transport system permease protein OpuCD
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheH
Sporulation-specific N-acetylmuramoyl-L-alanine amidase
Probable ABC transporter permease protein YqgH
Uncharacterized N-acetyltransferase YsnE
Uncharacterized metallophosphoesterase YkoQ
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Positions MEP for the nucleophilic attack
Putative ribosomal N-acetyltransferase YdaF
Sporulation-specific transcription factor SpoVIF
Putative teichuronic acid biosynthesis glycosyltransferase TuaG
SPbeta prophage-derived uncharacterized protein YoqI
Glutamate synthase large subunit-like protein YerD
Cannot fulfill its catalytic role within PTS transport. Does not affect dimerization.
Cadmium, cobalt and zinc/H(+)-K(+) antiporter
Uncharacterized MFS-type transporter YhcA
Confers streptomycin resistance and very poor growth in rich medium.
In RPSL1 (KO-267); confers streptomycin resistance but not hyperaccurate translation, increases natural antibiotic production 10-fold.
In RPSL4 (STRA1); confers streptomycin resistance and hyperaccurate translation, has no effect on natural antibiotic production.
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YwtE
Putative ABC transporter substrate-binding lipoprotein YhfQ
Uncharacterized HTH-type transcriptional regulator YqaF
Glutamate-1-semialdehyde 2,1-aminomutase 2
Sporulation-killing factor biosynthesis protein SkfC
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase 1
Does not bind the ilvB and dpp promoters.
Global transcriptional regulator CodY C-terminal
Global transcriptional regulator CodY N-terminal
Is predominantly monomeric. Retains the capacity to bind to DNA and repress transcription in vivo, even though its activity is considerably lower than that of the wild-type strain.
Loss of ilvB repressing activity in a BCAA-containing medium.
Loss of ilvB repressing activity in a BCAA-containing medium. DNA binding is stimulated by GTP, but not by branched-chain amino acids.
Nearly complete loss of ilvB repressing activity in a BCAA-containing medium.
No change in affinity for ilvB and dpp promoters.
Retains 72% of ilvB repressing activity in a BCAA-containing medium.
Retains some ilvB repressing activity in a BCAA-containing medium.
Retains some ilvB repressing activity in a BCAA-containing medium. Retains the ability to respond to GTP, but responds poorly to branched-chain amino acids unless GTP is simultaneously present.
Slightly reduced affinity for ilvB and dpp promoters.
Strong defect in ability to interact with the ilvB and dpp promoters.
Strongly affects the ilvB repressing activity in a BCAA-containing medium. DNA binding is stimulated by GTP, but not by branched-chain amino acids.
Uncharacterized ABC transporter ATP-binding protein YthP
Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)
Probable molybdopterin-guanine dinucleotide biosynthesis adapter protein
Succinate dehydrogenase iron-sulfur subunit
Probable mannose-6-phosphate isomerase GmuF
Methionine import ATP-binding protein MetN
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Bacterial bifunctional deaminase-reductase C-terminal
Cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB
Aliphatic sulfonates import ATP-binding protein SsuB
In TS-21; temperature-sensitive, develops, at the nonpermissive temperature (47 degrees Celsius), a coccoid-like morphology, i.e. a phenotype associated with a deficient synthesis of poly(glycerol phosphate) teichoic acid.
Probable ABC transporter permease protein YesP
Uncharacterized HTH-type transcriptional regulator YdfF
Organic hydroperoxide resistance protein OhrA
SPbeta prophage-derived uncharacterized protein YopY
2-oxoisovalerate dehydrogenase subunit beta
Bifunctional muramidase/lytic transglycosylase CwlQ
Lacks both muramidase and transglycosylase activities.
Lacks both muramidase and transglycosylase activities. Confers a defect in swarming motility.
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Uncharacterized isochorismatase family protein YrdC
Marked diminution of methanol upon both addition and removal of asparagine. No release of methanol upon asparagine addition but methanol release upon asparagine removal is not affected; when associated with D-630. Release of methanol upon both asparagine addition and removal; when associated with D-637. No methylation; when associated with D-630 and D-637.
Marked diminution of methanol upon both addition and removal of asparagine; fails to adapt to addition of asparagine. Release of methanol upon both asparagine addition and removal; when associated with D-371. Releases methanol upon asparagine addition but not upon asparagine removal; when associated with D-630. No methylation; when associated with D-371 and D-630.
Marked diminution of methanol upon both addition and removal of asparagine; methanol release delayed by about 1 minute compared to wild-type; adapts normally to addition of asparagine but fails to adapt to asparagine removal. No release of methanol upon asparagine addition but methanol release upon asparagine removal is not affected; when associated with D-371. Releases methanol upon asparagine addition but not upon asparagine removal; when associated with D-637. No methylation; when associated with D-371 and D-637.
Methyl-accepting chemotaxis protein McpB
Mannitol-specific phosphotransferase enzyme IIA component
SPbeta prophage-derived uncharacterized protein YomS
Putative peptidyl-prolyl cis-trans isomerase YacD
Flagellar hook-length control protein-like C-terminal
Probable flagellar hook-length control protein
SPbeta prophage-derived uncharacterized protein YopU
ABC transporter guanosine-binding protein NupN
ABC transporter substrate-binding protein PnrA-like
SPbeta prophage-derived uncharacterized protein YorB
Sec-independent protein translocase protein TatAy
Putative major teichoic acid biosynthesis protein C
Glycoside hydrolase family 42 N-terminal
Hydrolysis of the covalent glycosyl-enzyme intermediate is slowed.
Bis(5'-nucleosyl)-tetraphosphatase PrpE [asymmetrical]
6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating
Putative HMP/thiamine-binding protein YkoF
Thiamin/hydroxymethyl pyrimidine-binding YkoF putative
ABC-type transporter ATP-binding protein EcsA
In ecsA-26; exoamylase and other exoenzymes decrease, competence- and sporulation-deficient phenotype.
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YopO
130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.
2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
Iron-uptake system permease protein FeuB
Type II restriction enzyme BsuMI component YdiS
Probable NADH-dependent butanol dehydrogenase 2
ABC transporter ATP-binding protein NatA
SPbeta prophage-derived uncharacterized membrane protein YomJ
Antilisterial bacteriocin subtilosin biosynthesis protein AlbA
No longer cleaves SAM, does not make thioether bonds, contains 68% iron of wild-type.
Weakly cleaves SAM but does not make thioether bonds, contains 68% iron of wild-type.
Alpha-ketoglutaric semialdehyde dehydrogenase
SPbeta prophage-derived uncharacterized protein YopK
Abolishes 3'-5' exonuclease activity. Still possesses DNA polymerase activity.
Putative NAD(P)H-dependent FMN-containing oxidoreductase YwqN
Required to activate transcription of the adhA-yraA operon.
Uncharacterized MFS-type transporter YwfA
Antilisterial bacteriocin subtilosin biosynthesis protein AlbG
Required for cell division in the presence of a transpeptidase-inactive copy of PBP-2B. Loss of oxacillin and cephalexin resistance.
10% of wild-type activity. 17-fold decrease in the affinity for the pyrimidine substrate, but no effect on that for 1-deoxy-L-glycero-tetrulose 4-phosphate.
Uncharacterized ABC transporter ATP-binding protein YcbN
Uncharacterized HTH-type transcriptional regulator YkoM
Spore germination protein-like protein YdzR
Sec-independent protein translocase protein TatAc
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
Succinate--CoA ligase [ADP-forming] subunit beta
Nicotinate phosphoribosyltransferase C-terminal
Nicotinate phosphoribosyltransferase N-terminal
Nicotinate/nicotinamide phosphoribosyltransferase
Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
High-affinity zinc uptake system protein ZnuA
Uncharacterized glycosyltransferase YfnE
Probable spore germination protein GerPF
SPbeta prophage-derived disulfide bond formation protein B
Galactooligosaccharides transport system permease protein GanP
Probable membrane transporter protein YdhB
Uncharacterized HTH-type transcriptional regulator YurK
May be essential to modulate pKa of the D-135 carboxyl group
Unsaturated rhamnogalacturonyl hydrolase YesR
Uncharacterized HTH-type transcriptional regulator YydK
Cytochrome b/b6 C-terminal region profile
Menaquinol:cytochrome c reductase cytochrome c subunit
NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Probable inorganic carbon transporter subunit DabB
SPbeta prophage-derived uncharacterized protein YopA
6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating
Probable glycolate oxidase iron-sulfur subunit
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
Large ribosomal subunit protein uL30-like ferredoxin-like fold
Retains less than 10% of wild-type activity.
Uncharacterized HTH-type transcriptional regulator YgzD
Arginine transport system permease protein ArtQ
Uncharacterized transcriptional regulatory protein YclJ
DNA-directed RNA polymerase subunit epsilon
Uncharacterized ABC transporter permease protein YvrB
Spore coat polysaccharide biosynthesis protein SpsF
SPbeta prophage-derived putative HNH endonuclease YoqL
Probable HTH-type transcriptional repressor ExuR
Putative niacin/nicotinamide transporter NiaP
D-alanyl-D-alanine carboxypeptidase DacF
SPbeta prophage-derived uncharacterized protein YolA
Uncharacterized ABC transporter ATP-binding protein YhcH
Uncharacterized N-acetyltransferase YfmK
Ribosomal RNA large subunit methyltransferase H
Almost loss of activity. Decreases tRNA binding.
Cannot form a dimer and has no activity; when associated with Glu-253.
Cannot form a dimer and has no activity; when associated with Glu-256.
Strong decrease in activity. Decreases tRNA binding.
Putative [ribosomal protein S5]-alanine N-acetyltransferase
Uncharacterized ABC transporter ATP-binding protein YxlF
SPbeta prophage-derived uncharacterized protein YonK
Organic hydroperoxide resistance protein OhrB
Guanosine ABC transporter permease protein NupP
Decrease in induction of sigma-B activity in response to a salt stress. In absence of the other RsbR paralogs no change in stress response when RsbRB.
Decrease in induction of sigma-B activity in response to a salt stress. In absence of the other RsbR paralogs no change in stress response.
No phosphorylation; when associated with A-171. In absence of the other RsbR paralogs unable to function as a co-antagonist when RsbRB.
No phosphorylation; when associated with A-205. No stress response. In absence of the other RsbR paralogs greatly reduced stress response.
Uncharacterized HTH-type transcriptional regulator YusO
Putative thiamine pyrophosphate-containing protein YdaP
Uncharacterized FAD-linked oxidoreductase YgaK
10-fold reduction in sporulation efficiency. Sigma factor G (SigG) is not activated, signal sequence is not cleaved.
200-fold reduction in sporulation efficiency.
7-fold reduction in sporulation efficiency (first SpoIVFB transmembrane region).
No change in sporulation efficiency (SleB signal sequence).
Carboxy-terminal processing protease CtpA
Strongly reduced catalytic activity and reduced affinity for substrate.
SPbeta prophage-derived uncharacterized protein YonA
Uncharacterized HTH-type transcriptional regulator YcnC
N-acetyl-alpha-D-glucosaminyl L-malate synthase
SPbeta prophage-derived uncharacterized protein YoqB
Intracellular exo-alpha-L-arabinofuranosidase 2
Uncharacterized HTH-type transcriptional regulator YyaN
DNA-directed RNA polymerase beta subunit external 1
DNA-directed RNA polymerase subunit 2 hybrid-binding
DNA-directed RNA polymerase subunit beta
Bacteria grow as well as wild-type, are slightly longer, protein no longer binds bocillin-FL (a penicillin analog). Increased sensitivity to cefoxitin, very slight increase in resistance to penicillin G and cephalexin.
Cells are viable and motile, however they are filamentous and only about 20% of cells produce spores. Less protein is made, still binds penicillin.
95% loss of activity, normal PLP binding capacity.
More than 95% loss of activity, and half of normal PLP binding capacity.
Prevents intramolecular processing to an active enzyme.
Cytochrome bd ubiquinol oxidase subunit 1
2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Ribulose bisphosphate carboxylase large subunit C-terminal
Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal
Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha
SPbeta prophage-derived uncharacterized protein YopR
Uncharacterized HTH-type transcriptional regulator YdcH
Iron(3+)-hydroxamate import system permease protein FhuB
Uncharacterized HTH-type transcriptional regulator YsmB
Gram-positive cocci surface proteins LPxTG
Pentaglycyl murein peptidoglycan amidated threonine
Uncharacterized HTH-type transcriptional regulator YisR
Uncharacterized aromatic compound monooxygenase YhjG
Broad specificity amino-acid racemase RacX
18% of wild-type activity, but 100-fold decrease in substrate affinity.
50% of wild-type activity, but 9-fold decrease in substrate affinity.
8% of wild-type activity, but 7-fold decrease in substrate affinity.
Complete loss of activity, and widespread change in 3D-structure.
Glycerol-3-phosphate cytidylyltransferase
Reduces affinity for glycerol 3-phosphate about 100-fold.
D-alanyl-D-alanine carboxypeptidase DacA
Uncharacterized HTH-type transcriptional regulator YusT
25% retention of activity. Two to three fold reduction in KM for acetyl-CoA. Three-fold slower turnover number. Retains 60% of the activity of the wild-type after heating it to 100 degrees Celsius for 30 minutes.
25% retention of activity. Two to three-fold reduction in KM for acetyl-CoA. Three-fold slower turnover number. Retains 54% of the activity of the wild-type after heating it to 100 degrees Celsius for 30 minutes.
Inactive. Leads to a rapid turnover of the enzyme.
Uncharacterized ABC transporter ATP-binding protein YdbJ
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagT
Retains the ability to degrade haem, albeit at a reduced rate relative to the wild-type.
UTP--glucose-1-phosphate uridylyltransferase YngB
Uncharacterized HTH-type transcriptional regulator YuzN
SPbeta prophage-derived uncharacterized protein YopH
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Probable NADH-dependent flavin oxidoreductase YqiG
N-acetylglucosamine-6-phosphate deacetylase
Sodium, potassium, lithium and rubidium/H(+) antiporter
Loss of activity. Reduces activity 500-fold, without effect on zinc binding; when associated with Q-56.
Loss of activity. Reduces zinc binding by 80%.
No effect on zinc binding. Strongly reduces Vmax.
No effect on zinc binding. Strongly reduces Vmax. Reduces activity 500-fold; when associated with H-53.
Uncharacterized cysteine-rich protein YhjQ
1000-fold decrease of the affinity for NrgAB compared to the wild-type. Structure more asymmetric than that of the wild-type.
Causes constitutive expression of TnrA. Feedback-inhibited GlnA has a greatly reduced ability to block the DNA binding.
Causes constitutive expression of TnrA. Feedback-inhibited GlnA has a greatly reduced ability to block the DNA-binding.
No interaction with glutamine synthetase.
Alanyl-transfer RNA synthetases family profile
Abolishes negative regulation of DNA binding, without significantly interfering with the Cu(1+) affinity or coordination geometry.
Copper-sensing transcriptional repressor CsoR
ATP-dependent protease ATPase subunit ClpY
Replication initiation protein-like C-terminal
Rolling Circle replication initiation protein N-terminal
Degradation enzyme regulation protein DegQ
Uncharacterized ATP-dependent helicase YprA
Uncharacterized HTH-type transcriptional regulator YvdE
Probable glucose-1-phosphate cytidylyltransferase
SPbeta prophage-derived uncharacterized protein YonU
Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding
Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic
Determinant for substrate specificity and/or activity
Increases accumulation of full-length MifM.
Increases accumulation of full-length MifM. Reduces misCB/yqjG induction in misCA/spoIIIJ mutant.
Membrane protein insertion and folding monitor
Reduces misCB/yqjG induction in misCA/spoIIIJ mutant.
Loss of most ability to inhibit sigma-G, no longer interacts with sigma-G, replace sequence with same region from C.acetobutylicum.
Loss of most sigma-G inhibition, still slight interaction with sigma-G.
No longer inhibits or interacts with sigma-G.
No longer inhibits sigma-G, still slight interaction with sigma-G.
No longer inhibits sigma-G. Restores 70% of sigma-G inhibition; when associated with 11-A--A-14.
No longer inhibits sigma-G. Restores 70% of sigma-G inhibition; when associated with 30-A--A-33.
Uncharacterized MFS-type transporter YvqJ
Uncharacterized MFS-type transporter YqjV
Uncharacterized RNA pseudouridine synthase YlyB
No phosphorylation by HPrK/P; abolishes the catabolite repressive effects of glucose on gluconate kinase, glucitol dehydrogenase, and the mannitol-specific catabolic enzymes.
Pros-phosphohistidine intermediate; alternate
Uncharacterized HTH-type transcriptional regulator YybE
SPbeta prophage-derived uncharacterized protein YolD
Large ribosomal subunit protein uL6 alpha-beta
SPbeta prophage-derived uncharacterized protein YotB
Sec-independent protein translocase protein TatCd
Manganese transport system membrane protein MntD
Magnesium transporter MgtE intracellular
Bifunctional muramidase/DL-endopeptidase CwlT
Loss of muramidase activity. Shows no detectable transfer of ICEBs1.
Loss of peptidase activity. 1000-fold decrease in conjugation efficiency.
No detectable change in conjugation efficiency.
SPbeta prophage-derived uncharacterized protein YonP
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YitU
Uncharacterized HTH-type transcriptional regulator YbbH
Loss of kinase activity towards RsbS. Loss of the ability to activate sigma-B in response to salt and ethanol stress while retaining the ability to respond to energy stress.
Large-conductance mechanosensitive channel
Uncharacterized ABC transporter ATP-binding protein YfmR
Putative membrane-bound acyltransferase YkrP
50% wild-type amount of Cys-thioether cross-link made.
Approximately wild-type amounts of Cys-thioether cross-link made.
Cyclic, disulfide-containing sactipeptide with a thioether cross-link of cysteine to methionine.
Cys-Met cross-link not made, suggests relative amino acid positions are important for thioether bond formation.
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Leads to a 13-fold reduction of CO(2) production.
Leads to a 20-fold reduction of CO(2) production.
Leads to a 25-fold reduction of activity and a 4-fold reduction of CO(2) production.
Uncharacterized acyl-CoA thioester hydrolase YkhA
Probable N-acetyl-LL-diaminopimelate aminotransferase
SPbeta prophage-derived endonuclease YokF
Probable spore germination lipoprotein YhcN
Loss of catalytic activity. Unable to form an octanoyl-enzyme intermediate.
Octanoyl-[GcvH]:protein N-octanoyltransferase
Ribonucleoside-diphosphate reductase NrdEB subunit alpha, 1st part
Ribonucleoside-diphosphate reductase NrdEB subunit alpha, 2nd part
Methionine import system permease protein MetP
Uncharacterized HTH-type transcriptional regulator YbzH
Uncharacterized isochorismatase family protein YddQ
SPbeta prophage-derived uncharacterized protein YonD
Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal
Carbamoyl-phosphate synthase arginine-specific large chain
Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase
3-methyl-2-oxobutanoate hydroxymethyltransferase
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Polyketide biosynthesis cytochrome P450 PksS
May play a role in metal incorporation in vivo
May play a role in transmitting local conformational changes
Putative Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YjdI
Polyribonucleotide nucleotidyltransferase
Uncharacterized HTH-type transcriptional regulator RghRB
Sodium/calcium exchanger membrane region
Drastic decrease in arabinanase activity. Does not unduly disrupt the calcium cluster. Mutation does not disrupt the calcium cluster, but it relaxes the geometry of the cluster, resulting in a more planar arrangement of the calcium ion with five of the water molecules.
Extracellular endo-alpha-(1->5)-L-arabinanase 2
Extracellular endo-alpha-(1->5)-L-arabinanase C-terminal
Redox-sensing transcriptional repressor Rex
SPbeta prophage-derived uncharacterized protein YosD
Probable 3-hydroxyacyl-CoA dehydrogenase
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
UDP-N-acetylenolpyruvoylglucosamine reductase
SPbeta prophage-derived uncharacterized protein YomT
TVP38/TMEM64 family membrane protein YtxB
Putative molybdenum transport system permease protein YvgM
Restores reduced sporulation efficiency in a phrA deletion mutant.
Retains partial activity toward RapA and shows activity toward RapB.
31 X 21 AA approximate tandem repeats of X(4)-G-X(4)-[YF]-X-D-X(2)-G-X(4)
Uncharacterized metallophosphoesterase YkuE
scyllo-inositol 2-dehydrogenase (NAD(+))
Uncharacterized zinc-type alcohol dehydrogenase-like protein YogA
Probable glycine dehydrogenase (decarboxylating) subunit 1
Protease synthase and sporulation protein PAI 2
Glycosyl hydrolase family 30 beta sandwich
Cryptic catabolic NAD-specific glutamate dehydrogenase GudB
In gudB1; gains glutamate dehydrogenase activity, restores growth on proline, arginine, ornithine.
7-fold decrease in induction of SdpI, decreased toxicity of SDP.
Reduced amounts of pro-SdpC produced, no proSdpC is secreted, decreased amounts of SdpI expressed, decreased toxicity.
Asparagine synthetase [glutamine-hydrolyzing] 1
Putative HMP/thiamine permease protein YkoE
Putative phosphinothricin acetyltransferase YwnH
Iron-uptake system permease protein FeuC
Glycosyl transferase family 28 C-terminal
Spore coat polysaccharide biosynthesis protein SpsG
Probable siderophore transport system ATP-binding protein YusV
DnaJ homologue subfamily C member 28 conserved
Recognizes anti-sigma-G factor Gin (csfB)
Uncharacterized HTH-type transcriptional regulator YkvN
SPbeta prophage-derived uncharacterized protein YomK
Assimilatory nitrate reductase electron transfer subunit
Polygalacturonan/rhamnogalacturonan transport system permease protein YtcP
Cadmium, zinc and cobalt-transporting ATPase
Uncharacterized isochorismatase family protein YaaI
Uncharacterized N-acetyltransferase YbbJ
Sporulation initiation inhibitor protein Soj
Uncharacterized N-acetyltransferase YoaA
Maltodextrin transport system permease protein MdxF
Uncharacterized ABC transporter ATP-binding protein YhcG
Putative competence-damage inducible protein
SPbeta prophage-derived uncharacterized protein YotI
Alkaline phosphatase synthesis transcriptional regulatory protein PhoP
Uncharacterized HTH-type transcriptional regulator YxbF
Orn/Lys/Arg decarboxylases family 1 pyridoxal-P attachment site
Uncharacterized sodium-dependent transporter YocS
SPbeta prophage-derived uncharacterized protein YorO
SPbeta prophage-derived uncharacterized membrane protein YosW
Uncharacterized HTH-type transcriptional regulator YkvZ
D-alanyl-D-alanine carboxypeptidase DacC
Involved in allosteric activation by GTP
Loss of cooperativity with ATP. 5-fold decrease in affinity for UMP. Increased sensitivity to activation by GTP.
Loss of cooperativity with ATP. Increased sensitivity to activation by GTP.
Putative N-acetylmuramoyl-L-alanine amidase YrvJ
2-fold decrease in affinity and very low decrease in catalytic efficiency.
Low decrease in catalytic efficiency and in affinity.
Melibiose/raffinose/stachyose-binding protein MelE
Uncharacterized membrane protein YdzM/YouB
2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase
Trifunctional nucleotide phosphoesterase protein YfkN
In gutR1; results in the constitutive expression of gutB.
Uncharacterized ABC transporter ATP-binding protein YvcR
Required for deposition of coat proteins
Required for the assembly of CotA, CotR and YaaH
Required for the assembly of CotG and CotB
Required for the assembly of CotS and CotSA
Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Does not complement an ftsH deletion, loss of ATPase activity.
Does not complement an ftsH deletion, loss of protease activity against casein.
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
Transition state regulatory protein AbrB
Probable molybdenum cofactor guanylyltransferase
SPbeta prophage-derived uncharacterized protein YorE
Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Hydroxymethylglutaryl-coenzyme A synthase C-terminal
Hydroxymethylglutaryl-coenzyme A synthase N-terminal
Polyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG
PTS system oligo-beta-mannoside-specific EIIC component
Arabinose metabolism transcriptional repressor
No effect on glycosylation; when associated with A-42.
No effect on glycosylation; when associated with Q-40.
SPbeta prophage-derived bacteriocin sublancin-168
Gluconate operon transcriptional repressor
Can restore growth of E.coli secA mutants.
Forms position 587-750 dimers upon oxidation in vitro; when associated with C-587. Also forms position 750-750 dimers (homodimers).
Forms position 587-750 dimers upon oxidation in vitro; when associated with C-750. Does not form position 587-587 dimers (homodimers).
Forms position 588-588 dimers upon oxidation in vitro (homodimers).
Loss of activity. Cannot complement E.coli secA mutants.
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-80 and Q-83.
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-80 and Q-86.
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-83 and Q-86.
Weakens the interaction with Bacillus phage SP01 Gp46.
Putative sensory transducer protein YvaQ
SPbeta prophage-derived uncharacterized protein YomW
Flagellar hook-basal body complex protein FlhP
Uncharacterized MscS family protein YfkC
Uncharacterized HTH-type transcriptional regulator YozG
Not toxic in E.coli, 100-fold decreased RNA-binding.
Not toxic in E.coli, 50-fold decreased RNA-binding.
Not toxic in E.coli, 625-fold decreased RNA-binding.
Not toxic in E.coli, 70-fold decreased RNA-binding.
Putative metabolite transport protein YyaJ
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
N-acetyl-S-alkylcysteine sulfoxide monooxygenase
Loss of methylase activity, still binds DNA.
Uncharacterized HTH-type transcriptional regulator YcxD
Uncharacterized ABC transporter ATP-binding protein YknY
SPbeta prophage-derived uncharacterized protein YoqU
DNA polymerase III delta subunit C-terminal
Loss of activity. Cannot complement the null mutant.
Reduces the farnesylation activity using FPP as the prenyl donor, but geranylation activity using GPP is comparable to that of the wild-type enzyme.
Phage terminase large subunit C-terminal
Phage terminase large subunit N-terminal
tRNA N6-adenosine threonylcarbamoyltransferase
Uncharacterized ATP-dependent helicase YqhH
Probable peptide export ATP-binding protein YydI
SPbeta prophage-derived deoxyuridine 5'-triphosphate nucleotidohydrolase YosS
SPbeta prophage-derived recombinase-like protein YomM
2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
Ribose-phosphate pyrophosphokinase N-terminal
Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
Uncharacterized isochorismatase family protein YwoC
6-phospho-5-dehydro-2-deoxy-D-gluconate aldolase
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheI
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase N-terminal
SPbeta prophage-derived uncharacterized protein YorW
Homooligomerizes poorly, decreased number of foci.
Uncharacterized PIN and TRAM-domain containing protein YacL
Uncharacterized HTH-type transcriptional regulator YdeL
Uncharacterized transcriptional regulatory protein YbdJ
HPr(Ser) kinase/phosphorylase N-terminal
Important for the catalytic mechanism of both phosphorylation and dephosphorylation
Important for the catalytic mechanism of dephosphorylation
Loss of both kinase and phosphorylase activities.
Nearly no effect on both kinase and phosphorylase activities.
No effect on ATP-dependent kinase activity, but loss of both PPi-kinase and phosphorylase activities.
No effect on both kinase and phosphorylase activities.
Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity
Reduced kinase activity and loss of phosphorylase activity.
Reduced kinase activity and loss of phosphorylase activity. No effect on FBP binding.
Slight reduction in both kinase and phosphorylase activities.
Uncharacterized SPbeta prophage-derived protein YoqJ
Loss of activity in vivo, about 100-fold loss in vitro.
Loss of activity, significant loss of rRNA binding.
Putative HTH-type transcriptional regulator YwnA
Uncharacterized transcriptional regulatory protein YhcZ
Peptide methionine sulfoxide reductase MsrA
Peptide methionine sulphoxide reductase MsrA
PTS system N-acetylglucosamine-specific EIICB component
SPbeta prophage-derived uncharacterized protein YosG
Uncharacterized zinc-type alcohol dehydrogenase-like protein YycR
Holliday junction branch migration complex subunit RuvA
Uncharacterized HTH-type transcriptional regulator YbfI
Putative enoyl-CoA hydratase/isomerase YngF
Uncharacterized MFS-type transporter YtbD
Probable glutamine ABC transporter permease protein GlnM
DNA-binding transcriptional activator AdeR
Assimilatory nitrite reductase [NAD(P)H] small subunit
Putative PTS system EIIBC component YbbF
Impairs cleavage of the yitJ riboswitch.
Increased protein expression (replaces with transmembrane helix from EzrA).
SPbeta prophage-derived uncharacterized protein YorM
Putative methyl-accepting chemotaxis protein YoaH
N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase
SPbeta prophage-derived uncharacterized protein YomD
Prokaryotic-type class I peptide chain release factors
Probable coenzyme A transferase subunit alpha
Putative ABC transporter substrate-binding protein YesO
Uncharacterized ABC transporter permease protein YtlD
Uncharacterized HTH-type transcriptional regulator YwbI
Acetoin dehydrogenase operon transcriptional activator AcoR
Phosphoribosylformylglycinamidine synthase linker
Phosphoribosylformylglycinamidine synthase subunit PurL
Pyrimidine nucleoside phosphorylase C-terminal
Probable glucitol transport protein GutA
Spore coat polysaccharide biosynthesis protein SpsK
Uncharacterized zinc-type alcohol dehydrogenase-like protein YjmD
Loss of activity. Decreases iron binding. Cannot complement the deletion mutant. Lacks activity and does not bind iron; when associated with S-318.
Loss of activity. Does not bind iron. Cannot complement the deletion mutant.
No change in activity and in iron content.
No change in activity and in iron content. Cannot complement the deletion mutant. Lacks activity and does not bind iron; when associated with S-320.
Probable rhamnogalacturonan acetylesterase YesY
Uncharacterized HTH-type transcriptional regulator YbfP
Glyceraldehyde-3-phosphate dehydrogenase 1
Putative cytochrome bd menaquinol oxidase subunit II
SPbeta prophage-derived uncharacterized protein YopZ
Response regulator aspartate phosphatase I
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
23S rRNA (guanosine(2553)-2'-O)-methyltransferase RlmP
RNA 2-O ribose methyltransferase substrate binding
SPbeta prophage-derived uncharacterized protein YorC
Uncharacterized MFS-type transporter YfkF
In spo-1; no spores develop, no induction of sporulation-associated enzymes.
In spo-560; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-561; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-563; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-564; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-578; no spores develop, no induction of sporulation-associated enzymes.
Uncharacterized N-acetyltransferase YkwB
Bacterial type II secretion system protein E
Cells no longer transformable but still undergo competence-associated growth arrest; protein localizes to the cell pole.
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YotL
PTS system maltose-specific EIICB component
Probable amino-acid-binding protein YxeM
Uncharacterized MFS-type transporter YcxA
Uncharacterized ABC transporter ATP-binding protein YknV
Intracellular proteinase inhibitor BsuPI
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-2 or A,Q-4. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-2 and A,Q-4.
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-2 or A,Q-5. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-2 and A,Q-5.
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-4 or A,Q-5. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-4 and A,Q-5.
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-?)
Response regulator aspartate phosphatase A
Retains phosphatase activity, but is insensitive to PhrA inhibition.
Sporulation-defective phenotype. Retains phosphatase activity, but is insensitive to PhrA inhibition.
Polyketide biosynthesis acyltransferase homolog PksD
Essential for the oxygen-regulated activity
Teichoic acid glycerol-phosphate primase
Lactate utilization protein B C-terminal
Charge relay system; for urate oxidase activity
Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
Proton donor; for OHCU decarboxylase activity
Uric acid degradation bifunctional protein PucL
Petrobactin import ATP-binding protein YclP
ATPase F1/V1/A1 complex alpha/beta subunit N-terminal
ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding
Glutamyl-tRNA(Gln) amidotransferase subunit A
Spore morphogenesis and germination protein YwcE
Uncharacterized MscS family protein YhdY
Uncharacterized RNA methyltransferase YfjO
In rpsD1; suppresses S12 mutation K55D. A ram mutation.
In rpsD2; suppresses S12 mutation K55D. A ram mutation.
Small ribosomal subunit protein uS4 N-terminal
Polyketide biosynthesis acyl-carrier-protein AcpK
SPbeta prophage-derived pesticidal crystal protein-like YokG
L-cystine import ATP-binding protein TcyN
In sow-10; suppresses the motility loss and flagellar assembly defect of an fliW deletion.
In sow-11; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-12; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-15; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-2 and sow28; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-20; suppresses the motility loss and flagellar assembly defect of an fliW deletion.
In sow-8 and sow-4; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
Inhibits motility and flagellar filament assembly, greatly reduced binding of FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW.
Inhibits motility and flagellar filament assembly, reduced binding of FliW.
Inhibits motility and flagellar filament assembly, still binds FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW.
Probable serine/threonine-protein kinase YbdM
Biotin carboxyl carrier protein of acetyl-CoA carboxylase
Carbamoyl-phosphate synthase arginine-specific small chain
Imidazole glycerol phosphate synthase subunit HisF
SPbeta prophage-derived uncharacterized protein YoqX
Uncharacterized HTH-type transcriptional regulator YobD
Galactooligosaccharides transport system permease protein GanQ
Abolished D-alanylation of lipoteichoic acid (LTA).
Does not affect D-alanylation of lipoteichoic acid (LTA).
Negative regulator of genetic competence ClpC/MecB
Putative phosphotransferase enzyme IIA component YyzE
Uncharacterized MFS-type transporter YfiU
18-fold reduction in p-Arg phosphatase activity and 11-fold increase in p-Tyr phosphatase activity.
18-fold reduction in p-Arg phosphatase activity and 22-fold increase in p-Tyr phosphatase activity.
60-fold reduction in p-Arg phosphatase activity and 4-fold reduction in p-Tyr phosphatase activity.
Completely loss of phosphatase activity.
Translation elongation factor P/YeiP central
Branched-chain-amino-acid aminotransferase 2
Uncharacterized ABC transporter ATP-binding protein YkpA
Loss of debacillithiolation of S-bacillithiolated Bdr (Bdr-SSB).
Important in orienting the L-aspartate substrate
Uncharacterized ABC transporter ATP-binding protein YurJ
Glycosyltransferase family 28 N-terminal
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Uncharacterized MFS-type transporter YdeR
SPbeta prophage-derived uncharacterized protein YomG
Uncharacterized ABC transporter permease YclI
Oligopeptide transport system permease protein AppB
SPbeta prophage-derived uncharacterized protein YouA
SPbeta prophage-derived uncharacterized protein YoqM
tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Forms flat, unwrinkled biofilm. Biofilm retains nonwetting, hydrophobic nature.
Forms flat, unwrinkled biofilm. Loss of colony hydrophobicity.
Partial loss of morphological complexity. Biofilm retains nonwetting, hydrophobic nature.
SPbeta prophage-derived uncharacterized lipoprotein YokB
Putative TrmH family tRNA/rRNA methyltransferase YacO
Uncharacterized metallophosphoesterase-like protein YydB
Probable glycine dehydrogenase (decarboxylating) subunit 2
Uncharacterized transmembrane protein YxlG
30% endonuclease and 10% 5'-exonuclease activity.
Loss of endo- and 5'-exonuclease activity.
Required for endo- and 5'-exonuclease activity and dimerization
Severe loss of endo- and 5'-exonuclease activity.
Slight decrease in endonuclease and 10-fold decrease in 5'-exonuclease activity; when associated with A-337.
Slight decrease in endonuclease and 10-fold decrease in 5'-exonuclease activity; when associated with A-45.
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Dipeptide transport ATP-binding protein DppD
SPbeta prophage-derived uncharacterized protein YoqC
Causes mislocalization and strongly impairs sporulation. Does not affect membrane association.
Does not affect localization or sporulation.
Slightly affects localization and sporulation.
Specifically impairs cortex assembly, but not the initiation of coat assembly. Strongly impairs sporulation, but does not affect localization.
Strongly impairs sporulation, but does not affect localization.
Uncharacterized HTH-type transcriptional regulator YobS
Loss of activity. Affects the oligomeric state.
UDP-N-acetylbacillosamine N-acetyltransferase
SPbeta prophage-derived uncharacterized protein YorZ
Teichuronic acid biosynthesis protein TuaE
Cytochrome bd ubiquinol oxidase subunit 2
SPbeta prophage-derived sublancin-168-processing and transport ATP-binding protein SunT
Uncharacterized MFS-type transporter YcnB
Glucose-6-phosphate dehydrogenase C-terminal
Glucose-6-phosphate dehydrogenase NAD-binding
Phosphoribosylglycinamide formyltransferase
Could be involved in aa-tRNA binding(Leu) binding
Could have a critical role in the catalytic mechanism
Uncharacterized ABC transporter ATP-binding protein YhaQ
DEAD-box ATP-dependent RNA helicase CshB
Uncharacterized N-acetyltransferase YitH
Grows slowly, very reduced association with ribosomes, fewer 70S ribosomes in cells. No effect on sporulation or the general stress response.
No effect on growth or ribosomes, eliminates sporulation onset. Also decreases the general stress response to physical stress.
Stops growing at 45 degrees Celsius, shows sporulation onset defects. KM for GTP is 2.3 uM, turnover number is 0.015/min.
Probable flagellar assembly protein FliH
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YopS
3-oxoacyl-[acyl-carrier-protein] reductase FabG
ATP synthase F1 complex delta/epsilon subunit N-terminal
Accumulation of S-bacillithiolated form due to lack of this resolving cysteine residue. Debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) without the prior NaBH(4) reduction of this protein in vitro. Debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Retains isomerization activity of the disulfide bonds in scrambled tachyplesin substrate. Loss of isomerization activity; when associated with A-53.
Loss of debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) by the NaBH(4)-reduced form of this protein in vitro. Loss of debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Retains isomerization activity of the disulfide bonds in scrambled tachyplesin substrate. Loss of isomerization activity; when associated with A-55.
tRNA (adenine(22)-N(1))-methyltransferase
SPbeta prophage-derived uncharacterized protein YorP
In divIVA1; loss of function resulting in sporulation defect and in the formation of minicells.
Localization at cytoplasmic foci instead of the cell pole. Stable interaction with Spo0J and association with the chromosome.
Lack of phosphorylation. No chemotaxis, tumbly swimming behavior and shows exclusively clockwise rotation.
No chemotaxis, tumbly swimming behavior and shows exclusively clockwise rotation.
Single-stranded-DNA-specific exonuclease RecJ
Single-stranded-DNA-specific exonuclease RecJ C-terminal
Probable L-serine dehydratase, beta chain
Uncharacterized HTH-type transcriptional regulator YwrC
Branched-chain amino acid transport protein AzlC
Carbamoyl-phosphate synthase pyrimidine-specific large chain
Probable membrane transporter protein YunE
Charge relay system; for autoendoproteolytic cleavage activity
Cleavage (non-hydrolytic); by autocatalysis
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
No longer inhibits growth when overexpressed in relA mutants, probably does not synthesisize (p)ppGpp. 70S ribosomes no longer dimerize to form 100S ribosomes.
No longer inhibits growth when overexpressed in relA mutants.
Cysteine sulfenic acid (-SOH); alternate
N,N-(cysteine-1,S-diyl)phenylalanine (Cys-Phe); alternate
Organic hydroperoxide resistance transcriptional regulator
S-bacillithiol cysteine disulfide; alternate
SPbeta prophage-derived uncharacterized protein YopI
Hypoxanthine-guanine phosphoribosyltransferase
Plays an important role in substrate specificity
In sapB2 and sapB10; activates alkaline phosphatase during sporulation.
SPbeta prophage-derived uncharacterized protein YorF
No effect on cell shape or cell viability.
Peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK
Putative Rieske 2Fe-2S iron-sulfur protein YhfW
Probable iron-sulfur-binding oxidoreductase FadF
Loss of enzymatic activity. Fails to form the normal DisA focus. Cannot trigger the DNA damage response. Increased sensitivity to methyl methanesulfonate.
Reduction in the capability of the four-way junction DNA to inhibit diadenylate cyclase. Reduction in DNA-binding.
No activity; no imine formation with DXP.
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
(S)-ureidoglycine--glyoxylate transaminase
5-fold decrease in transamination activity.
9-fold decrease in transamination activity.
SPbeta prophage-derived uncharacterized protein YoqK
Signal recognition particle receptor FtsY
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Uncharacterized glycosyltransferase YqgM
Peptide methionine sulfoxide reductase MsrB
Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead
Probable xanthine dehydrogenase subunit D
Glycerol phosphate lipoteichoic acid synthase 1
Processed glycerol phosphate lipoteichoic acid synthase 1
Branched-chain-amino-acid transaminase 1
Poly(glycerol-phosphate) alpha-glucosyltransferase
Release factor glutamine methyltransferase
Release factor glutamine methyltransferase N-terminal
In absence of the other RsbR paralogs decreased stress response.
In absence of the other RsbR paralogs no change in stress response.
In absence of the other RsbR paralogs small decrease in stress response.
Uncharacterized HTH-type transcriptional regulator YfiR
Gamma-glutamylcyclotransferase AIG2-like
Putative gamma-glutamylcyclotransferase YkqA
SPbeta prophage-derived DNA ligase-like protein LigB
Glutamate-1-semialdehyde 2,1-aminomutase
Uncharacterized N-acetyltransferase YuaI
Loss of ability to recognize wild-type spoVG promoter. Suppressor of the spoVG249 mutation in the -10 region of the spoVG promoter.
Suppressor of mutations in the -12 region of the spoVG promoter.
Suppressor of mutations in the -13 region of the spoVG promoter.
Probable peptide export permease protein YydJ
3-phosphoshikimate 1-carboxyvinyltransferase
Flagellar hook-associated protein 2 C-terminal
Flagellar hook-associated protein 2 N-terminal
2-oxoglutarate dehydrogenase E1 component
Short-chain dehydrogenase/reductase homolog YusS
Putative sigma L-dependent transcriptional regulator YplP
Probable ABC transporter permease protein YurM
Putative ABC transporter ATP-binding protein AlbC
Putative sensory transducer protein YfmS
Putative metabolite transport protein YwtG
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
Petrobactin import system permease protein YclO
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
Heat-inducible transcription repressor HrcA
Heat-inducible transcription repressor HrcA C-terminal
Holliday junction branch migration complex subunit RuvB
SPbeta prophage-derived uncharacterized protein YoqF
Ribonucleoside-diphosphate reductase subunit beta
ATP-dependent Clp protease ATP-binding subunit ClpX
Uncharacterized HTH-type transcriptional regulator YuxN
Uncharacterized MFS-type transporter YfmI
Putative arsenical pump membrane protein
Uncharacterized N-acetyltransferase YycN
Tryptophan RNA-binding attenuator protein inhibitory protein
ADP-dependent (S)-NAD(P)H-hydrate dehydratase
Uncharacterized transcriptional regulatory protein YesN
Glycine betaine/carnitine/choline transport ATP-binding protein OpuCA
Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-7.
Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-8.
Uncharacterized ABC transporter ATP-binding protein YknU
Bifunctional non-homologous end joining protein LigD
Succinate-semialdehyde dehydrogenase [NADP(+)]
Uncharacterized UDP-glucosyltransferase YojK
Uncharacterized HTH-type transcriptional regulator YobV
SPbeta prophage-derived uncharacterized protein YopV
Grows slowly, cells have abnormal morphology.
Higher endogenous GTPase activity, GTPase more stimulated by 70S ribosomes than wild-type, increased association with 30S ribosomal subunit in vitro, wild-type behavior in vivo.
Loss of most phosphorylation by PrkC, GTPase activity less stimulated by 70S ribosomes than wild-type, decreased association with 30S ribosomal subunit in vitro. Grows slowly, cells have abnormal morphology.
Loss of phosphorylation by PrkC, but PrkC autophosphorylation considerably decreased in vitro.
Loss of phosphorylation by PrkC, but PrkC autophosphorylation greatly decreased in vitro.
No GTPase activity. Wild-type phosphorylation by PrkC in vitro.
Small ribosomal subunit biogenesis GTPase RsgA
Wild-type phosphorylation by PrkC in vitro.
Prevents a complete closure of the inter-domain cleft, affecting ATP binding, ATP hydrolysis and RNA unwinding.
Slows down the catalytic cycle, but does not affect formation of a closed conformer and global conformation; when associated with A-182.
Slows down the catalytic cycle, but does not affect formation of a closed conformer and global conformation; when associated with A-184.
Still adopts a closed conformation upon binding of ATP and RNA, but lacks ATPase and RNA unwinding activities.
Methylthioribulose-1-phosphate dehydratase
Slight induction of WalK-dependent genes.
Two-component system WalR/WalK regulatory protein YycH
Putative teichuronic acid biosynthesis glycosyltransferase TuaH
Sec-independent protein translocase protein TatAd
Changes the activity from exo- to endo-cleavage.
Oligopeptide transport ATP-binding protein OppD
1-pyrroline-5-carboxylate dehydrogenase 2
Uncharacterized HTH-type transcriptional regulator YezE
Uncharacterized glycosyltransferase YdaM
Severely reduces ATPase activity and growth rate.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Uncharacterized HTH-type transcriptional regulator YdgC
Uncharacterized UDP-glucose epimerase YtcB
SPbeta prophage-derived uncharacterized protein YopL
Nicotinate-nucleotide adenylyltransferase
Uncharacterized FAD-linked oxidoreductase YitY
SPbeta prophage-derived uncharacterized protein YorI
Does not complement the tagTUV deletion mutant.
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagU
Probable spore germination lipoprotein YlaJ
Probable glucosamine-6-phosphate deaminase 2
Putative HMP/thiamine import ATP-binding protein YkoD
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
Aminodeoxychorismate/anthranilate synthase component 2
Uncharacterized HTH-type transcriptional regulator YxaD
Uncharacterized sodium-dependent transporter YocR
Polygalacturonan/rhamnogalacturonan-binding protein YtcQ
Important for catalytic activity and assists the phosphoryl transfer reaction to Asp8 by balancing charge and orienting the reacting groups
Probable N-acetyl-alpha-D-glucosaminyl L-malate deacetylase 2
SPbeta prophage-derived uncharacterized protein YoqY
Methyl-accepting chemotaxis protein TlpA
Maltodextrin transport system permease protein MdxG
Uncharacterized MFS-type transporter YdgK
Purine nucleoside transport protein NupG
Putative pyridoxal phosphate-dependent aminotransferase EpsN
Stabilizes the active site histidine for nucleophilic attack
Beta-ketoacyl-[acyl-carrier-protein] synthase III 2
Sporulation-specific extracellular nuclease
Probable siderophore transport system permease protein YfhA
In BPSC 31 and BPSC 61; spectinomycin resistant.
In rpsE1; spectinomycin restistant, not a ram mutant.
In rpsE7; suppresses S12 mutant K55D. A ram mutation.
In rpsE8; suppresses S12 mutant K55D. RspE8 is lethal in the absence of the S12 mutation.
In rpsE9; suppresses S12 mutation K55D. Lethal in the absence of the S12 mutation.
Spermine/spermidine N(1)-acetyltransferase
Probable 5-dehydro-4-deoxyglucarate dehydratase
Could play a key role in the communication between the regulatory and the substrate sites
Uncharacterized UDP-glucosyltransferase YdhE
Involved in Mg(2+) ion dislocation from EF-Tu
Translation elongation factor EFTs/EF1B dimerisation
SPbeta prophage-derived DNA-binding protein HU 2
RDXXRA motif of the pArg binding pocket involved in allosteric regulation
Stabilizes quantity of CtsR to an extent a little lower to that observed for the mcsB deletion, indicating a decrease of protein phosphorylation activity of McsB in vivo.
Stabilizes quantity of CtsR to an extent comparable to that observed for the mcsB deletion, indicating a loss of protein phosphorylation activity of McsB in vivo.
The protein does not accumulate and cells grow very slowly.
Probable multidrug resistance protein NorM
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
Uncharacterized ATP-dependent helicase YwqA
SPbeta prophage-derived uncharacterized protein YoqN
Uncharacterized ABC transporter permease YvcS
Putative component of 'biosynthetic module'
In sboA1; protein has acquired hemolytic activity and is more effective against tested Gram-positive bacteria.
In vitro can no longer make the Cys-thioether cross-link, variable loss of the other 2 thioether cross-links, in vivo no cyclopeptide formed.
In vitro no longer makes the first thioether cross-link, in vivo no cyclopeptide formed.
In vitro no longer makes the second thioether cross-link, in vivo no cyclopeptide formed.
In vivo forms 2 thioether cross-links, no cyclopeptide formed.
In vivo forms 3 thioether cross-links, forms the cyclopeptide.
Transcription termination/antitermination protein NusA
Uncharacterized N-acetyltransferase CgeE
Putative FAD-dependent monooxygenase YetM
Uncharacterized NAD(P)H oxidoreductase YdeQ
Oligopeptide transport ATP-binding protein AppF
Schiff-base intermediate with acetaldehyde
Guanosine import ATP-binding protein NupO
DNA-directed RNA polymerase RpoA/D/Rpb3-type
DNA-directed RNA polymerase subunit alpha
Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
Probable ATP-dependent RNA helicase YfmL
SPbeta prophage-derived uncharacterized protein YotJ
PTS system oligo-beta-mannoside-specific EIIA component
Catalytic mutant, no alteration in rRNA binding.
Retains nuclease activity, still stimulated by L3.
Probable guanidinium efflux system subunit GdnC
SPbeta prophage-derived putative antirepressor protein YoqD
Uncharacterized HTH-type transcriptional regulator YcgE
Branched-chain amino acid transport protein AzlD
Energy-coupling factor transporter transmembrane protein EcfT
Can bind ComA but is insensitive to PhrF inhibition.
Can bind ComA. Interacts with RapF, and can also interact with PhrC.
Can bind ComA. Interacts with RapF, but not with PhrC.
Completely suppresses expression from srfA promoter and is insensitive to PhrF inhibition.
Significant decrease in ComA binding activity.
Uncharacterized HTH-type transcriptional regulator YhcF
Ribosomal RNA small subunit methyltransferase H
SPbeta prophage-derived uncharacterized protein YomX
Uncharacterized HTH-type transcriptional regulator YczG
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Flagellar motor switch protein FliG C-terminal
Flagellar motor switch protein FliG N-terminal
Flagellar motor switch protein FliG middle
Uncharacterized MFS-type transporter YwoD
Uncharacterized MFS-type transporter YjbB
Imidazole glycerol phosphate synthase subunit HisH
Cell wall-active antibiotics response LiaF-like C-terminal
Probable membrane transporter protein YjnA
Glycine betaine transport ATP-binding protein OpuAA
scyllo-inositol 2-dehydrogenase (NADP(+)) IolU
Putative [ribosomal protein S18]-alanine N-acetyltransferase
Large ribosomal subunit protein bL12 C-terminal
Large ribosomal subunit protein bL12 oligomerization
Large ribosomal subunit assembly factor BipA
In dnaD23; temperature-sensitive mutant that affects the initiation of chromosome replication.
Shikimate dehydrogenase substrate binding N-terminal
SPbeta prophage-derived uncharacterized protein YopT
Probable polysaccharide deacetylase PdaB
Uncharacterized FAD-linked oxidoreductase YvdP
Uncharacterized HTH-type transcriptional regulator YmfC
Short-chain dehydrogenase/reductase homolog YusR
Does not affect septal localization and growth.
Does not affect septal localization; when associated with R-37 and R-41.
Does not affect septal localization; when associated with R-37 and R-44.
Does not affect septal localization; when associated with R-41 and R-44.
Small ribosomal subunit protein bS1 homolog
SPbeta prophage-derived uncharacterized protein YomN
PTS system mannitol-specific EIICB component
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Deoxyuridine 5'-triphosphate nucleotidohydrolase YncF
Glutathione hydrolase-like YwrD large chain
Glutathione hydrolase-like YwrD small chain
Antilisterial bacteriocin subtilosin biosynthesis protein AlbB
L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferase
Does not significantly affect alpha-galacturonidase activity.
Contains heme B and heme A at levels comparable to that of wild-type CtaA.
Contains small amounts of heme B and heme A.
Decrease in heme A synthase activity and 5-fold reduction in cytochrome c oxidase activity. Contains heme B, heme O, and heme I, as well as trace amounts of heme A.
Inactive. Contains heme B and heme O, with mainly heme O bound to CtaA.
Binds potassium in the micromolar range.
SPbeta prophage-derived uncharacterized protein YopE
Iron(3+)-hydroxamate import system permease protein FhuG
May be essential to modulate pKa of the D-143 carboxyl group
Unsaturated rhamnogalacturonyl hydrolase YteR
Induction of srfA is delayed approximately 1 generation.
Induction of srfA is delayed by 2 generations or more.
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit A
Bifunctional cytochrome P450/NADPH--P450 reductase 1
Exhibits modified substrate specificity. Shows approximately 6- to 9-fold increased activity with SDS, lauric acid and 1,4-naphthoquinone, and enhanced activity for other substrates such as ethacrynic acid and epsilon-amino-n-caproic acid.
SPbeta prophage-derived uncharacterized protein YomE
Sigma factor SigB regulation protein RsbQ
Putative tRNA (cytidine(34)-2'-O)-methyltransferase
Protein-glutamate methylesterase/protein-glutamine glutaminase
Putative sigma L-dependent transcriptional regulator YqiR
N5-carboxyaminoimidazole ribonucleotide synthase
4-hydroxy-tetrahydrodipicolinate reductase
Dihydrodipicolinate reductase C-terminal
Dihydrodipicolinate reductase N-terminal
L-Lysine--8-amino-7-oxononanoate transaminase
Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM
Phenylalanine--tRNA ligase alpha subunit
Probable ribosome maturation protein RlbA
Probable coenzyme A transferase subunit beta
Uncharacterized glycosyltransferase YkoT
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YonR
Probable ABC transporter extracellular-binding protein YckB
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS
Putative propionyl-CoA carboxylase beta chain
Probable formaldehyde dehydrogenase AdhA
Uncharacterized transcriptional regulatory protein YvcP
Probable biofilm-surface layer protein B
SPbeta prophage-derived uncharacterized protein YoqR
Uncharacterized MFS-type transporter YybF
ATP-dependent helicase/nuclease subunit A
Loss of helicase and nuclease activity. DNA-binding is unaltered.
Some loss of nuclease activity, helicase and DNA-binding are unaltered. No production of chi fragments; when associated with A-961 in AddB nearly complete loss of nuclease activity.
Uncharacterized MFS-type transporter YxiO
RNA polymerase sigma factor 54 DNA-binding
RNA polymerase sigma factor 54 core-binding
Uncharacterized HTH-type transcriptional regulator YtcD
Response regulator aspartate phosphatase E
Decreased affinity for protoporphyrinogen-IX.
Reduces protoporphyrinogen oxidation by 90%.
Reduces protoporphyrinogen oxidation by 99%.
Uncharacterized MFS-type transporter YbcL
Arabinooligosaccharides transport system permease protein AraP
Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.
Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
Almost abolishes the glycosyltransferase activity.
Drastic loss of activity for both pterostilbene glycosylation and UDP glycosylation.
Enhances the catalytic efficiency of UDP glycosylation, but reduces the relative activity of pterostilbene glycosylation reaction.
Increases UDP glycosylation efficiency and enhances pterostilbene glycosylation activity.
Increases pterostilbene glycosylation activity and UDP glycosylation efficiency.
Leads to about 2-fold decrease in specific activities.
No change in activity. Dramatic reduction of the catalytic efficiency and glycosylation levels; when associated with A-128.
No change in activity. Dramatic reduction of the catalytic efficiency and glycosylation levels; when associated with A-129.
Significant decrease in activity for both pterostilbene glycosylation and UDP glycosylation.
Significant reduction in catalytic efficiency and glycosylation levels.
Significantly enhances the UDP glycosylation efficiency, but shows unchanged or reduced pterostilbene glycosylation when supplied with UDP-glucose and ADP-glucose, respectively.
Strong decrease in activity for both pterostilbene glycosylation and UDP glycosylation.
SPbeta prophage-derived uncharacterized protein YotM
Putative acetyl-CoA C-acetyltransferase YhfS
Loss of protease activity, but mutant is still able to swarm.
SPbeta prophage-derived uncharacterized protein YonE
Aminoacyl-tRNA synthetase class I anticodon-binding
Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic
Loss of copper binding; when associated with S-13.
Loss of copper binding; when associated with S-16.
Multidrug-efflux transporter 1 regulator
Does not affect secretion and dimerization.
Lack of secretion, but does not affect dimerization.
Probable amino-acid permease protein YxeN
PTS system fructose-specific EIIB component
Probable peptidoglycan endopeptidase LytE
Spore coat polysaccharide biosynthesis protein SpsA
DEAD-box ATP-dependent RNA helicase CshA
Required for dimerization or oligomerization
SPbeta prophage-derived uncharacterized protein YoqG
Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-227.
Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-228.
Prevents phosphorylation and increases ATPase activity; when associated with F-227 and F-228.
Large ribosomal subunit protein uL11 C-terminal
Large ribosomal subunit protein uL11 N-terminal
Exonuclease VII large subunit C-terminal
Sigma-K factor-processing regulatory protein BofA
Uncharacterized MFS-type transporter YwoG
Loss of phosphorylation and loss of the ability to respond to salt stress. Interacts strongly with RsbT.
Putative DNA-3-methyladenine glycosylase YfjP
10-fold decrease in catalytic efficiency with ABTS as substrate. 91-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
165-fold decrease in catalytic efficiency with ABTS as substrate. 400-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
25-fold decrease in catalytic efficiency with ABTS as substrate. 30-fold decrease in catalytic efficiency with SGZ as substrate.
33-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
357-fold decrease in catalytic efficiency with ABTS as substrate. 152-fold decrease in catalytic efficiency with SGZ as substrate.
5-fold decrease in catalytic efficiency with ABTS as substrate. 785-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
6-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
9-fold decrease in catalytic efficiency with ABTS as substrate. 26-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
9-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
Decreases copper content. Strong decrease in catalytic efficiency with both ABTS and SGZ.
Does not affect copper content. Strong decrease in catalytic efficiency with both ABTS and SGZ.
Loss of laccase activity. Mutant fails to develop the dark brown phenotype typical of the wild type strain.
Loss of laccase activity. Mutant fails to develop the dark brown phenotype typical of the wild type strain. Decreases copper content.
Plays a crucial role in the protonation steps
Retains approximately 20% of the wild-type activity with both ABTS and SGZ.
Retains approximately 30% of the wild-type activity with SGZ but does not affect activity with ABTS.
Retains approximately 50% of the wild-type activity with both ABTS and SGZ.
Retains approximately 60% of the wild-type activity with both ABTS and SGZ.
Retains approximately 70% of the wild-type activity with both ABTS and SGZ.
Slight decrease in catalytic efficiency. Shows minimal changes in the structure of the copper centers.
Strong decrease in catalytic efficiency. Significant differences in both the type 1 and type 2 copper centers.
Histidine biosynthesis bifunctional protein HisIE
PTS system glucose-specific EIICBA component
SPbeta prophage-derived uncharacterized protein YotC
SPbeta prophage-derived uncharacterized protein YomU
Bifunctional cytochrome P450/NADPH--P450 reductase 2
Hydroxylates shorter substrates with higher conversion rates than wild-type, and in contrast to wild-type, is also able to convert medium-chain alkanes and aromatic compounds; when associated with Q-190.
Hydroxylates shorter substrates with higher conversion rates than wild-type, and in contrast to wild-type, is also able to convert medium-chain alkanes and aromatic compounds; when associated with V-89.
Putative bacteriocin-SkfA transport system permease protein SkfF
Uncharacterized HTH-type transcriptional regulator YtdP
SPbeta prophage-derived uncharacterized protein YopW
Extracellular matrix regulatory protein B
SPbeta prophage-derived uncharacterized protein YokC
SPbeta prophage-derived uncharacterized protein YomL
Two-component system WalR/WalK regulatory protein YycI
Uncharacterized amino acid permease YtnA
SPbeta prophage-derived uncharacterized protein YopM
Uncharacterized RNA pseudouridine synthase YtzG
SPbeta prophage-derived putative HNH homing endonuclease YosQ
Fe(3+)-citrate import system permease protein YfmD
9 X 13 AA approximate tandem repeats of [HY]-K-K-S-[HYFC]-[RC]-[TS]-[HY]-K-K-S-R-S
Uncharacterized HTH-type transcriptional regulator YisV
3-oxo-glucose-6-phosphate:glutamate aminotransferase
2-fold reduced cleavage of SigEI-17 mutant.
60% reduced cleavage of SigEI-17 mutant.
70% reduced cleavage of SigEI-17 mutant.
75% reduced cleavage of SigEI-17 mutant.
80% reduced cleavage of SigEI-17 mutant.
80% reduced cleavage of SigEI-17 mutant. Abolishes cleavage of SigEI-17 mutant; when associated with G-296.
Abolishes cleavage of SigEI-17 mutant; when associated with N-298.
Abolishes cleavage of wild-type SigE. Abolishes cleavage of SigEI-17 mutant.
Nearly unaffected cleavage of SigEI-17 mutant.
Sporulation sigma-E factor-processing peptidase
Unaffected cleavage of wild-type and K-25 mutant SigE in sporulating B.subtilis cells (PubMed:8002606). Abolishes cleavage of wild-type and K-25 mutant SigE when proteins are coexpressed in E.coli cells (PubMed:21362630).
Could be important to modulate the pK values of the two catalytic cysteine residues
O-acetylserine dependent cystathionine beta-synthase
Sigma-G-dependent sporulation-specific SASP protein
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
Uncharacterized ABC transporter ATP-binding protein YvrO
Abolishes cytochrome c oxidase assembly.
The measured mass is closest to that of the mature protein with 2 palmitate moieties.
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Metalloregulation DNA-binding stress protein
High-affinity zinc uptake system membrane protein ZnuB
Putative sodium/proton-dependent alanine carrier protein YrbD
Peptide of residues 247-265 no longer binds FliW.