No homotrimer formation. Stimulates ATPase activity of RFC, no stimulation of DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers.
Proliferating cell nuclear antigen PCNA C-terminal
Proliferating cell nuclear antigen PCNA N-terminal
Small amount of homotrimer in solution. Stimulates ATPase activity of RFC and DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers.
Aspartate/homoserine dehydrogenase NAD-binding
Mannose-6-phosphate isomerase type II C-terminal
No longer complements secY24, an E.coli temperature-sensitive secY mutation.
Reduces enzymatic activity by 96% at 37 degrees Celsius and by 88% at 87 degrees Celsius; when associated with A-161.
Reduces enzymatic activity by 96% at 37 degrees Celsius and by 88% at 87 degrees Celsius; when associated with A-173.
Alpha-D-phosphohexomutase alpha/beta/alpha
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with D-211, D-213 and A-223.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and A-223.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and D-213.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-213 and A-223.
Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-254.
Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-256.
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Molybdopterin cofactor biosynthesis C (MoaC)
Probable cyclic pyranopterin monophosphate synthase
Phosphoadenosine phosphosulphate reductase
DNA protection during starvation protein
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit
Thiamine pyrophosphate enzyme TPP-binding
Tryptophan synthase beta chain-like PALP
Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding
Nascent polypeptide-associated complex protein
N-(5'-phosphoribosyl)anthranilate isomerase
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
1,4-alpha-glucan branching enzyme C-terminal
Glycoside hydrolase family 57 N-terminal
Phosphoribosylformylglycinamidine synthase linker
Phosphoribosylformylglycinamidine synthase subunit PurL
UPF0173 metal-dependent hydrolase PF1764
2.4-fold decrease in substrate affinity.
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
6-hydroxymethylpterin diphosphokinase MptE-like
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
Glutamate synthase alpha subunit C-terminal
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Cobalamin-independent methionine synthase MetE C-terminal/archaeal
20-fold decrease in HJ cleavage, decreased binding to HJDNA.
No HJ cleavage. Slight decrease in DNA binding.
No HJ cleavage; at very high levels altered cleavage patterns. Dimerizes poorly.
No Holliday junction (HJ) cleavage, does not bind HJDNA.
Wild-type HJ cleavage. Slight decrease in DNA binding.
Glycosyltransferase subfamily 4-like N-terminal
Aldehyde ferredoxin oxidoreductase N-terminal
Tungsten-containing aldehyde ferredoxin oxidoreductase
DNA mismatch repair proteins mutS family
Molybdate/tungstate transport system permease protein WtpB
Peptidase S9 prolyl oligopeptidase catalytic
NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal
Putative HTH-type transcriptional regulator TrmBL2
Molybdate/tungstate import ATP-binding protein WtpC
Translation initiation factor 2 subunit alpha
Peptidase M1 membrane alanine aminopeptidase
Prephenate dehydrogenase nucleotide-binding
DNA-directed RNA polymerase subunit 2 hybrid-binding
Phosphatidic acid phosphatase type 2/haloperoxidase
GMP synthase [glutamine-hydrolyzing] subunit A
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
5-methylthioadenosine/S-adenosylhomocysteine deaminase
NADH:ubiquinone oxidoreductase-like 20kDa subunit
Ribose-phosphate pyrophosphokinase N-terminal
Pyruvate/ketoisovalerate oxidoreductase catalytic
Pyruvate/ketoisovalerate oxidoreductases common subunit gamma
Dinitrogenase iron-molybdenum cofactor biosynthesis
Aminoacyl-transfer RNA synthetases class-II family profile
Bacterial type II secretion system protein E
Spo11/DNA topoisomerase VI subunit A N-terminal
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Uncharacterized HTH-type transcriptional regulator PF0250
RNA 3'-terminal phosphate cyclase insert
SUF system FeS cluster assembly SufBD N-terminal
Glutamyl-tRNA(Gln) amidotransferase subunit E
DNA-directed DNA polymerase family B exonuclease
DNA-directed DNA polymerase family B multifunctional
3-isopropylmalate dehydratase large subunit 2
Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha
Probable Brix domain-containing ribosomal biogenesis protein
S-adenosyl-L-homocysteine hydrolase NAD binding
UDP-glucose/GDP-mannose dehydrogenase C-terminal
DNA repair and recombination protein RadA
Alanyl-transfer RNA synthetases family profile
Probable translation initiation factor IF-2, 1st part
Probable translation initiation factor IF-2, 2nd part
UDP-glucose/GDP-mannose dehydrogenase N-terminal
S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal
S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal
3-isopropylmalate dehydratase large subunit 1
SAM-dependent methyltransferase TRM5/TYW2-type
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Large ribosomal subunit protein uL30-like ferredoxin-like fold
Quinolinate phosphoribosyl transferase C-terminal
Quinolinate phosphoribosyl transferase N-terminal
Transcription factor NikR nickel binding C-terminal
Ribulose bisphosphate carboxylase large subunit C-terminal
Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal
Large ribosomal subunit protein uL15/eL18
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Removed in mature form; by autocatalysis
Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal
Pseudouridine synthase I TruA alpha/beta
Na+/H+ antiporter MnhB subunit-related protein
Transcription regulator AsnC/Lrp ligand binding
Dolichyl-phosphooligosaccharide-protein glycotransferase 2
Interacts with target acceptor peptide in protein substrate
Glucose-6-phosphate isomerase prokaryote
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Translation initiation factor 2 subunit beta
Imidazole glycerol phosphate synthase subunit HisF
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Extradiol ring-cleavage dioxygenase class III enzyme subunit B
Probable glycine cleavage system H protein
Histidine biosynthesis bifunctional protein HisIE
Phosphate import ATP-binding protein PstB
Glyceraldehyde-3-phosphate:ferredoxin oxidoreductase
Putative transcriptional regulatory protein PF0535
Polyribonucleotide 5'-hydroxyl-kinase Clp1 P-loop
Polyribonucleotide 5'-hydroxyl-kinase PF0112
Major facilitator superfamily (MFS) profile
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Docks into pockets in the proteasome alpha-ring to cause gate opening
O-(5'-phospho-DNA)-tyrosine intermediate
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
tRNA (uracil(54)-C(5))-methyltransferase
Fe-S hydro-lyase tartrate dehydratase beta-type catalytic
Putative glycogen debranching enzyme N-terminal
Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic
Pyridoxal 5'-phosphate synthase subunit PdxT
Membrane-bound hydrogenase subunit alpha
Putative [LysW]-lysine/[LysW]-ornithine hydrolase
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Putative 1-aminocyclopropane-1-carboxylate deaminase
Anthranilate synthase component I N-terminal
Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core
Acetate--CoA ligase [ADP-forming] II subunit beta
Methylated DNA-protein cysteine methyltransferase ribonuclease H-like
Methylated-DNA--protein-cysteine methyltransferase
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
Myo-inositol-1-phosphate synthase GAPDH-like
Ketoisovalerate oxidoreductase subunit VorB
DNA double-strand break repair helicase HerA
Ketoisovalerate oxidoreductase subunit VorA
Digeranylgeranylglyceryl phosphate synthase
Damage-control phosphatase ARMT1-like metal-binding
DNA-directed RNA polymerase M/15kDa subunit
Acetate--CoA ligase [ADP-forming] I subunit beta
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
N(4)-bis(aminopropyl)spermidine synthase C-terminal
Schiff-base intermediate with dihydroxyacetone-P
Acetate--CoA ligase [ADP-forming] I subunit alpha
HicB-like antitoxin of toxin-antitoxin system
Aconitase A/isopropylmalate dehydratase small subunit swivel
Small zinc finger protein HVO-2753-like zinc-binding pocket
Small ribosomal subunit protein uS4 N-terminal
DNA-directed RNA polymerase subunit Rpo6
Large ribosomal subunit protein uL2 C-terminal
Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom
Putative ABC transporter ATP-binding protein PF0528
Moderate decrease in cleavage of target RNA in the whole Cmr complex.
Nearly complete loss of cleavage of target RNA in the whole Cmr complex.
Significant decrease in cleavage of target RNA in the whole Cmr complex.
2-iminobutanoate/2-iminopropanoate deaminase
Important for catalytic activity at high pH
Probable transposase IS891/IS1136/IS1341
Involved in ionization of N3 of dUMP, leading to its activation
Uncharacterized HTH-type transcriptional regulator PF2053
Acetate--CoA ligase [ADP-forming] II subunit alpha
tRNA nucleotidyltransferase substrate binding
tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Phosphoribosylformylglycinamidine cyclo-ligase
Probable glycine dehydrogenase (decarboxylating) subunit 1
Peptidase A24A-predicted C-terminal archaea
Pyridine nucleotide-disulphide oxidoreductase dimerisation
23S rRNA (uracil(747)-C(5))-methyltransferase
DNA polymerase alpha/delta/epsilon subunit B
Glycoside hydrolase family 42 N-terminal
CobB/CobQ-like glutamine amidotransferase
Probable ribosomal RNA small subunit methyltransferase A
Ribosomal RNA adenine methylase transferase N-terminal
Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
Glyceraldehyde-3-phosphate dehydrogenase
95% reduction in activity compared to wild type.
Nucleophile; O-isoaspartyl threonine intermediate
Shows improved enzymatic properties at physiological conditions (pH 7.4 and 37 degrees Celsius) as compared to the wild type with a 2-fold increase in catalytic efficiency. Shows higher and significant killing of human leukemic and breast carcinoma cell lines as compared to the E.coli L-asparaginase.
Shows improved enzymatic properties at physiological conditions (pH 7.4 and 37 degrees Celsius) as compared to the wild type with a 2.6-fold increase in catalytic efficiency. Shows higher and significant killing of human leukemic and breast carcinoma cell lines as compared to the E.coli L-asparaginase.
Imidazole glycerol phosphate synthase subunit HisH
Large ribosomal subunit protein uL11 C-terminal
Large ribosomal subunit protein uL11 N-terminal
Putative translation initiation factor eIF-2B subunit 2-like
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Reduced ATP binding and enzyme activity; when associated with A-531.
Reduced ATP binding and enzyme activity; when associated with A-534.
Probable glycine dehydrogenase (decarboxylating) subunit 2
Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 1st part
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 2nd part
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 3rd part
Putative ABC transporter ATP-binding protein PF0068
DNA polymerase II large subunit DP2 N-terminal
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
Phosphoribosylformylglycinamidine synthase subunit PurQ
DNA-directed RNA polymerase subunit Rpo5
RNA polymerase subunit H/Rpb5 C-terminal
Putative type 4B encapsulin shell protein PF1875
Small ribosomal subunit protein uS15 N-terminal
Cysteine sulfenic acid (-SOH) intermediate
Interchain (with Cys-211); in linked form
Interchain (with Cys-46); in linked form
Polysaccharide biosynthesis protein C-terminal
Aspartate/ornithine carbamoyltransferase Asp/Orn-binding
Aspartate/ornithine carbamoyltransferase carbamoyl-P binding
Becomes active at low temperatures; when associated with C-228 or D-241.
Becomes active at low temperatures; when associated with G-278.
Increased dissociation of dodecamers into trimers.
Ornithine carbamoyltransferase, anabolic
DNA-directed RNA polymerase RpoA/D/Rpb3-type
DNA-directed RNA polymerase subunit Rpo3
Aminoacyl-tRNA synthetase class I anticodon-binding
ABC transporter substrate-binding protein PnrA-like
Putative HTH-type transcriptional regulatory protein PF1851
Ribosomal RNA small subunit methyltransferase Nep1
Cytochrome C biogenesis protein transmembrane
Sugar fermentation stimulation protein C-terminal
Sugar fermentation stimulation protein homolog
Pyruvate ferredoxin oxidoreductase beta subunit C-terminal
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
ATPase F1/V1/A1 complex alpha/beta subunit N-terminal
ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding
Carbamoyl-phosphate synthase small chain
Carbamoyl-phosphate synthase small subunit N-terminal
Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr), no activity on L-threonyl-tRNA(Thr).
Digeranylgeranylglycerophospholipid reductase
Uncharacterized HTH-type transcriptional regulator PF0864
Putative methylthioribose-1-phosphate isomerase
Thiamine pyrophosphate enzyme N-terminal TPP-binding
3-isopropylmalate dehydratase small subunit 2
NADH:ubiquinone oxidoreductase 30kDa subunit
O-(3'-phospho-DNA)-tyrosine intermediate
Transcription factor CBF/NF-Y/archaeal histone
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
Adenosylcobinamide-GDP ribazoletransferase
Glycine cleavage T-protein C-terminal barrel
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
DNA-directed RNA polymerase subunit Rpo1C
Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal
DNA double-strand break repair Rad50 ATPase
Decreases ATP-induced dimerization. Increases DNA end tethering.
Destabilizes the ATP-dimerized state. Promotes HerA/NurA activity.
Increases ATP-induced dimerization. Increases DNA end tethering. Fails to promote HerA/NurA activity.
Prevents ATP binding and disrupts the communication among the other ATP-binding loops. Prevents also Rad50 dimerization. Decreases both ATPase and adenylate kinase activities.
Uncharacterized HTH-type transcriptional regulator PF1734
Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange
Probable molybdenum cofactor guanylyltransferase
tRNA (cytidine(56)-2'-O)-methyltransferase
Signal recognition particle 19 kDa protein
Ribosome maturation protein SDO1/SBDS C-terminal
Ribosome maturation protein SDO1/SBDS N-terminal
Ribosome maturation protein SDO1/SBDS central
Gamma-glutamylcyclotransferase AIG2-like
Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase
Shikimate dehydrogenase substrate binding N-terminal
Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding
Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic
Uncharacterized HTH-type transcriptional regulator PF1231
Nicotinate phosphoribosyltransferase C-terminal
Large ribosomal subunit protein uL5 C-terminal
Large ribosomal subunit protein uL5 N-terminal
tRNA (pseudouridine(54)-N(1))-methyltransferase
Aspartate carbamoyltransferase regulatory chain
Aspartate carbamoyltransferase regulatory subunit C-terminal
Aspartate carbamoyltransferase regulatory subunit N-terminal
ADP-dependent glucose/glucosamine kinase
rRNA small subunit methyltransferase F RNA-binding PUA-like
Methylene-tetrahydrofolate reductase C-terminal
3-isopropylmalate dehydratase small subunit 1
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Polymerase/histidinol phosphatase N-terminal
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
D-glutamate N-acetyltransferase-like C-terminal
D-glutamate N-acetyltransferase-like N-terminal
Translation initiation factor 2 subunit gamma
Loss of interaction with Cmr2, no cleavage of target RNA when assembled in the Cmr complex.
Loss of interaction with Cmr2, still cleaves target RNA when assembled in the Cmr complex.
No cleavage of target RNA when assembled in the Cmr complex.
Still cleaves target RNA when assembled in the Cmr complex.
Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase
No effect on the efficiency of electron transfer.
The efficiency of electron transfer is compromised.
The mixed spin cluster is converted into a homogeneous spin cluster (S=3/2).
DNA-directed RNA polymerase subunit Rpo10
HTH-type sugar sensing transcriptional regulator TrmBL1
Putative CRISPR-associated endoribonuclease-like protein Cas6
Putative [LysW]-aminoadipate/[LysW]-glutamate kinase
3-methyl-2-oxobutanoate hydroxymethyltransferase
Probable membrane-bound hydrogenase subunit mbhJ
NAD(P)H sulfur oxidoreductase (CoA-dependent)
Glutamyl-tRNA(Gln) amidotransferase subunit D
Resolvase/invertase-type recombinase catalytic
Signal recognition particle 54 kDa protein
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-831.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-831 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-796; A-831 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-795; A-796; A-831 and A-835.
Replication factor C small subunit, 1st part
Replication factor C small subunit, 2nd part
Phenylalanine--tRNA ligase alpha subunit
Large ribosomal subunit protein uL6 alpha-beta
Nicotinamide-nucleotide adenylyltransferase
Pyruvate phosphate dikinase AMP/ATP-binding
Pyrimidine nucleoside phosphorylase C-terminal
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
Large ribosomal subunit protein uL10-like insertion
Glycosyl transferase family 3 N-terminal
DNA-directed RNA polymerase RBP11-like dimerisation
DNA-directed RNA polymerase subunit Rpo11
Translation elongation factor IF5A C-terminal
Carbamoyl-phosphate synthase large chain
Ketoisovalerate oxidoreductase subunit VorD
Probable cobalamin biosynthesis protein CobD
Plays an important role in substrate specificity
Phosphoenolpyruvate carboxykinase C-terminal P-loop
Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal
DNA-directed RNA polymerase subunit Rpo12
Uncharacterized HTH-type transcriptional regulator PF0054
tRNA N6-adenosine threonylcarbamoyltransferase
Formate-dependent phosphoribosylglycinamide formyltransferase
FAD-dependent oxidoreductase 2 FAD binding
Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal
Important in orienting the L-aspartate substrate