Electron transfer flavoprotein alpha subunit C-terminal
Electron transfer flavoprotein alpha/beta-subunit N-terminal
DNA-directed RNA polymerase RpoA/D/Rpb3-type
SUF system FeS cluster assembly SufBD N-terminal
Gfo/Idh/MocA-like oxidoreductase C-terminal
Gfo/Idh/MocA-like oxidoreductase N-terminal
Phosphocysteine intermediate; for EIIB activity
Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA
Major facilitator superfamily (MFS) profile
6-phosphogluconate dehydrogenase C-terminal
Terminase large subunit-like endonuclease
Polysaccharide biosynthesis protein C-terminal
Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
FAD-dependent oxidoreductase 2 FAD binding
Aspartate/ornithine carbamoyltransferase Asp/Orn-binding
Aspartate/ornithine carbamoyltransferase carbamoyl-P binding
Major facilitator superfamily (MFS) profile domain-containing protein
Hydroxymethylglutaryl-coenzyme A synthase C-terminal
Hydroxymethylglutaryl-coenzyme A synthase N-terminal
Glycoside hydrolase family 65 C-terminal
Glycoside hydrolase family 65 N-terminal
Glycoside hydrolase family 65 central catalytic
Alpha-D-phosphohexomutase alpha/beta/alpha
Threonine/serine exporter-like N-terminal
DNA mismatch repair proteins mutS family
Pyrroline-5-carboxylate reductase catalytic N-terminal
Pyrroline-5-carboxylate reductase dimerisation
Important for beta-aspartyl-AMP intermediate formation
Thymidylate synthase/dCMP hydroxymethylase
Peptidase S33 tripeptidyl aminopeptidase-like C-terminal
Type IV methyl-directed restriction enzyme EcoKMcrB subunit DNA-binding
Thiamin pyrophosphokinase thiamin-binding
Phosphatidic acid phosphatase type 2/haloperoxidase
Pyridoxamine kinase/Phosphomethylpyrimidine kinase
Phosphoribosylformylglycinamidine synthase linker
Ribose-phosphate pyrophosphokinase N-terminal
Pyridine nucleotide-disulphide oxidoreductase dimerisation
Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha
Ionotropic glutamate receptor C-terminal
Solute-binding protein family 3/N-terminal
Phosphomannose isomerase type I catalytic
Aminoacyl-transfer RNA synthetases class-II family profile
Septum formation inhibitor MinC C-terminal
CobB/CobQ-like glutamine amidotransferase
Peptidase S11 D-Ala-D-Ala carboxypeptidase A C-terminal
serine-type D-Ala-D-Ala carboxypeptidase
Beta-xylosidase C-terminal Concanavalin A-like
Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate
Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal
Beta-ketoacyl-[acyl-carrier-protein] synthase III N-terminal
Anthranilate synthase component I N-terminal
Gram-positive cocci surface proteins LPxTG
Gram-positive cocci surface proteins LPxTG domain-containing protein
Bacterial bifunctional deaminase-reductase C-terminal
Peptidase M15B domain-containing protein
Membrane insertase YidC/Oxa/ALB C-terminal
S-adenosylmethionine-dependent methyltransferase
D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding
D-isomer specific 2-hydroxyacid dehydrogenase catalytic
Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
Choloylglycine hydrolase/NAAA C-terminal
Solute-binding protein family 5 domain-containing protein
Nicotinate phosphoribosyltransferase C-terminal
Nicotinate phosphoribosyltransferase N-terminal
Nicotinate/nicotinamide phosphoribosyltransferase
Aconitase A/isopropylmalate dehydratase small subunit swivel
Schiff-base intermediate with acetaldehyde
Shikimate dehydrogenase substrate binding N-terminal
Cobalamin-independent methionine synthase MetE C-terminal/archaeal
Type VII secretion system protein EssD-like
Inosine/uridine-preferring nucleoside hydrolase
Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding
Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic
3-hydroxyisobutyrate dehydrogenase-like NAD-binding
6-phosphogluconate dehydrogenase NADP-binding
tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain
Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT C-terminal
S-adenosylmethionine synthetase C-terminal
S-adenosylmethionine synthetase N-terminal
Cation-transporting P-type ATPase N-terminal
Large ribosomal subunit protein uL2 C-terminal
Ribosome maturation factor RimP C-terminal
Ribosome maturation factor RimP N-terminal
Acyl-CoA dehydrogenase/oxidase C-terminal
Acyl-CoA dehydrogenase/oxidase N-terminal
DNA-directed RNA polymerase beta subunit external 1
DNA-directed RNA polymerase subunit 2 hybrid-binding
Large ribosomal subunit protein bL12 C-terminal
Large ribosomal subunit protein bL12 oligomerization
Resolvase/invertase-type recombinase catalytic
Carbamoyl-phosphate synthase small subunit N-terminal
Thiamine pyrophosphate enzyme N-terminal TPP-binding
Thiamine pyrophosphate enzyme TPP-binding
Could play a key role in the communication between the regulatory and the substrate sites
Alpha-L-rhamnosidase six-hairpin glycosidase
Single-stranded-DNA-specific exonuclease RecJ C-terminal
Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal
Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal
O-(phosphoribosyl dephospho-coenzyme A)serine
Peptide methionine sulphoxide reductase MsrA
N-(5'phosphoribosyl) anthranilate isomerase (PRAI)
Cell envelope-related transcriptional attenuator
Tryptophan synthase beta chain-like PALP
Small ribosomal subunit protein uS4 N-terminal
PTS EIIA type-1 domain-containing protein
Penicillin-binding protein transpeptidase
Mandelate racemase/muconate lactonizing enzyme C-terminal
Lysidine-tRNA(Ile) synthetase C-terminal
Phosphotransferase system EIIB component type 2/3
Integron-associated effector binding protein
Sigma 54 modulation/S30EA ribosomal protein C-terminal
Aspartate/homoserine dehydrogenase NAD-binding
Glucosamine/galactosamine-6-phosphate isomerase
ATPase F1/V1/A1 complex alpha/beta subunit N-terminal
ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding
Release factor glutamine methyltransferase N-terminal
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
DNA primase/polymerase bifunctional N-terminal
Large ribosomal subunit protein uL15/eL18
Chromosomal replication initiator DnaA C-terminal
Probable transposase IS891/IS1136/IS1341
Polymerase/histidinol phosphatase N-terminal
RNA binding; important for wobble base 34 recognition
YbaK/aminoacyl-tRNA synthetase-associated
Glycosyltransferase subfamily 4-like N-terminal
Tail specific protease domain-containing protein
O-(5'-phospho-DNA)-tyrosine intermediate
Calcineurin-like phosphoesterase domain-containing protein
Involved in allosteric activation by GTP
Translation elongation factor P/YeiP central
DNA polymerase III beta sliding clamp C-terminal
DNA polymerase III beta sliding clamp N-terminal
DNA polymerase III beta sliding clamp central
Alpha-glycerophosphate oxidase C-terminal
Aminoacyl-tRNA synthetase class I anticodon-binding
Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic
O-(3'-phospho-DNA)-tyrosine intermediate
Pyruvate phosphate dikinase AMP/ATP-binding
Exonuclease VII large subunit C-terminal
Glycosyl transferase family 3 N-terminal
Large ribosomal subunit protein uL6 alpha-beta
Translation elongation factor EFTs/EF1B dimerisation
Serine aminopeptidase S33 domain-containing protein
Glucose-6-phosphate dehydrogenase C-terminal
Glucose-6-phosphate dehydrogenase NAD-binding
Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich
Glycosyl hydrolases family 2 sugar binding
NIF system FeS cluster assembly NifU N-terminal
HPr(Ser) kinase/phosphorylase N-terminal
Important for the catalytic mechanism of both phosphorylation and dephosphorylation
Important for the catalytic mechanism of dephosphorylation
Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity
Peptidase M1 membrane alanine aminopeptidase
Phosphotransferase system enzyme I N-terminal
Helix-hairpin-helix DNA-binding motif class 1
Ribosomal RNA adenine methylase transferase N-terminal
Glycosyl transferase family 28 C-terminal
Glycosyltransferase family 28 N-terminal
Peptidase S9 prolyl oligopeptidase catalytic
DNA-directed DNA polymerase family A palm
Interaction with polymerase core subunit RpoC
Formamidopyrimidine-DNA glycosylase catalytic
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
NADH:flavin oxidoreductase/NADH oxidase N-terminal
Fe-S hydro-lyase tartrate dehydratase beta-type catalytic
DNA/pantothenate metabolism flavoprotein C-terminal
Phosphopantothenoylcysteine decarboxylase
Cobalamin-independent methionine synthase MetE N-terminal
Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic
Bacterial alpha-L-rhamnosidase N-terminal
Pyridoxamine 5'-phosphate oxidase putative
Large ribosomal subunit protein uL11 C-terminal
Large ribosomal subunit protein uL11 N-terminal
Conserved virulence factor B-like winged helix
ATP synthase F1 complex delta/epsilon subunit N-terminal
Positions ribitol 5-phosphate for the nucleophilic attack
RNA 2-O ribose methyltransferase substrate binding
Signal recognition particle SRP54 helical bundle
Important for interaction with partner proteins
Transcription elongation factor GreA/GreB C-terminal
Transcription elongation factor GreA/GreB N-terminal
Involved in proton transfer during cleavage
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase N-terminal
Peptidase S11 D-alanyl-D-alanine carboxypeptidase A N-terminal
Peptidase C39-like domain-containing protein
Large ribosomal subunit protein uL5 C-terminal
Large ribosomal subunit protein uL5 N-terminal
tRNA-specific 2-thiouridylase MnmA-like C-terminal
tRNA-specific 2-thiouridylase MnmA-like central
Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding
Glycoside hydrolase family 31 N-terminal
Polysaccharide chain length determinant N-terminal
Cyclophilin-like domain-containing protein
Galactose-1-phosphate uridyl transferase C-terminal
Galactose-1-phosphate uridyl transferase N-terminal
Glycoside hydrolase family 42 N-terminal
Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding
Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core
ATP-dependent DNA helicase RecG C-terminal
Important for autoinhibition of adenylyltransferase activity
Molybdopterin cofactor biosynthesis C (MoaC)
Ribosomal RNA small subunit methyltransferase E PUA-like
Ribosomal RNA small subunit methyltransferase E methyltransferase
Heat-inducible transcription repressor HrcA C-terminal
Important for catalytic activity and assists the phosphoryl transfer reaction to Asp8 by balancing charge and orienting the reacting groups
Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding
Dihydrodipicolinate reductase C-terminal
Dihydrodipicolinate reductase N-terminal
tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding
X-Prolyl dipeptidyl aminopeptidase PepX N-terminal
Prokaryotic-type class I peptide chain release factors
Large ribosomal subunit protein uL30-like ferredoxin-like fold
Sporulation transcription regulator WhiA N-terminal
Replication initiator protein A C-terminal
Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like
Pseudouridine synthase I TruA alpha/beta
Concentrative nucleoside transporter C-terminal
Concentrative nucleoside transporter N-terminal
Gamma-D-glutamyl-meso-diaminopimelate peptidase
Putative host cell surface-exposed lipoprotein Ltp-like HTH region
Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal
Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase N-terminal
Reversibly protonated during proton transport
Shows a significant FBP-induced thermostabilization as in the cases of many allosteric LDHs, indicating the binding of fructose 1,6-bisphosphate (FBP). However, the mutant is still a non-allosteric enzyme and shows essentially the same FBP-independent catalytic activity as the wild-type.
Plays an important role in substrate specificity
Gly-cisPro motif, important for rejection of L-amino acids