Complete DNA sequence of yeast chromosome II.
Formation of AP-3 transport intermediates requires Vps41 function.
Global analysis of protein expression in yeast.
Global analysis of protein localization in budding yeast.
Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex.
The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae.
A multidimensional chromatography technology for in-depth phosphoproteome analysis.
Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.
Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.
Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants.
Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.
Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.
The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes.
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
A single subunit, Dis3, is essentially responsible for yeast exosome core activity.
Reconstitution, activities, and structure of the eukaryotic RNA exosome.
The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames.
The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
The yeast exosome and human PM-Scl are related complexes of 3'-->5' exonucleases.
The yeast exosome functions as a macromolecular cage to channel RNA substrates for degradation.
A new system for naming ribosomal proteins.
Crystal structure of the eukaryotic ribosome.
Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions.
The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
The structure of the eukaryotic ribosome at 3.0 A resolution.
Complete DNA sequence of yeast chromosome XI.
Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8.
Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane.
LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway.
Molecular organization of target of rapamycin complex 2.
Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence.
RTG-dependent mitochondria to nucleus signaling is negatively regulated by the seven WD-repeat protein Lst8p.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.
Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control.
Analysis of a 70 kb region on the right arm of yeast chromosome II.
Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria.
Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide.
MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria.
Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography.
Sequencing and comparison of yeast species to identify genes and regulatory elements.
Structure of the yeast mitochondrial large ribosomal subunit.
Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.
The proteome of Saccharomyces cerevisiae mitochondria.
Parallel identification of new genes in Saccharomyces cerevisiae.
Quantitative trait loci mapped to single-nucleotide resolution in yeast.
Eukaryotic resistance to fluoride toxicity mediated by a widespread family of fluoride export proteins.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Yeast Fex1p is a constitutively expressed fluoride channel with functional asymmetry of its two homologous domains.
Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone.
Genomic analysis of PIS1 gene expression.
Sites of ubiquitin attachment in Saccharomyces cerevisiae.
A DNA integrity network in the yeast Saccharomyces cerevisiae.
A protein interaction map for cell polarity development.
A screen for genes required for meiosis and spore formation based on whole-genome expression.
Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I.
Role for perinuclear chromosome tethering in maintenance of genome stability.
Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex.
The complete DNA sequence of yeast chromosome III.
Investigation of two yeast genes encoding putative isoenzymes of phosphoglycerate mutase.
The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Transcriptional response to deletion of the phosphatidylserine decarboxylase Psd1p in the yeast Saccharomyces cerevisiae.
Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization.
Amyloid-beta peptide induces mitochondrial dysfunction by inhibition of preprotein maturation.
Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae.
Purification and characterization of protease M, a yeast mitochondrial nucleotide-stimulated metal protease: Its identification as CYM1 gene product, a mitochondrial presequence peptidase.
Role of the novel metallopeptidase Mop112 and saccharolysin for the complete degradation of proteins residing in different subcompartments of mitochondria.
Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
The Saccharomyces cerevisiae MTS1 gene encodes a putative RNA-binding protein involved in mitochondrial protein targeting.
Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase.
N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
Purification of ribonucleotide reductase subunits Y1, Y2, Y3, and Y4 from yeast: Y4 plays a key role in diiron cluster assembly.
Rnr4p, a novel ribonucleotide reductase small-subunit protein.
Structure of the yeast ribonucleotide reductase Y2Y4 heterodimer.
Structures of the yeast ribonucleotide reductase Rnr2 and Rnr4 homodimers.
Subcellular localization of yeast ribonucleotide reductase regulated by the DNA replication and damage checkpoint pathways.
Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit.
Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins.
A yeast TCP-1-like protein is required for actin function in vivo.
Assigning function to yeast proteins by integration of technologies.
Molecular characterization of Ypi1, a novel Saccharomyces cerevisiae type 1 protein phosphatase inhibitor.
Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.
Regulation of yeast mRNA 3' end processing by phosphorylation.
YPI1 and SDS22 proteins regulate the nuclear localization and function of yeast type 1 phosphatase Glc7.
Ypi1, a positive regulator of nuclear protein phosphatase type 1 activity in Saccharomyces cerevisiae.
A basic helix-loop-helix-leucine zipper transcription complex in yeast functions in a signaling pathway from mitochondria to the nucleus.
Nine-amino-acid transactivation domain: establishment and prediction utilities.
Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II.
Genetic control of recombination partner preference in yeast meiosis. Isolation and characterization of mutants elevated for meiotic unequal sister-chromatid recombination.
Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized morphogenesis signaling network in cell integrity.
Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins.
Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae.
Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis.
A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.
A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
Direct physical and functional interaction of the NuA4 complex components Yaf9p and Swc4p.
Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4.
The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres.
Analysis of a 23 kb region on the left arm of yeast chromosome IV.
Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.
Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.
The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae.
Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation.
Molecular architecture of a eukaryotic translational initiation complex.
The action of N-terminal acetyltransferases on yeast ribosomal proteins.
A conserved mediator hinge revealed in the structure of the MED7-MED21 (Med7-Srb7) heterodimer.
A high resolution protein interaction map of the yeast Mediator complex.
A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II.
Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV.
Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex.
Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts.
Mediator as a general transcription factor.
Mutual targeting of mediator and the TFIIH kinase Kin28.
Preponderance of free mediator in the yeast Saccharomyces cerevisiae.
Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction.
The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.
The structural and functional organization of the yeast mediator complex.
Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex.
Isofunctional enzymes PAD1 and UbiX catalyze formation of a novel cofactor required by ferulic acid decarboxylase and 4-hydroxy-3-polyprenylbenzoic acid decarboxylase.
New cofactor supports alpha,beta-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition.
PAD1 and FDC1 are essential for the decarboxylation of phenylacrylic acids in Saccharomyces cerevisiae.
Structure and mechanism of ferulic acid decarboxylase (FDC1) from Saccharomyces cerevisiae.
Purification and properties of ferrochelatase from the yeast Saccharomyces cerevisiae. Evidence for a precursor form of the protein.
The ferrochelatase from Saccharomyces cerevisiae. Sequence, disruption, and expression of its structural gene HEM15.
The nucleotide sequence of the ferrochelatase and tRNA(val) gene region from Saccharomyces cerevisiae.
SDHAF4 promotes mitochondrial succinate dehydrogenase activity and prevents neurodegeneration.
Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate.
Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae.
The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins.
A global topology map of the Saccharomyces cerevisiae membrane proteome.
A reticular rhapsody: phylogenic evolution and nomenclature of the RTN/Nogo gene family.
The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA.
HAT1 and HAT2 proteins are components of a yeast nuclear histone acetyltransferase enzyme specific for free histone H4.
Hat2p recognizes the histone H3 tail to specify the acetylation of the newly synthesized H3/H4 heterodimer by the Hat1p/Hat2p complex.
Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus.
Histone H3 and the histone acetyltransferase Hat1p contribute to DNA double-strand break repair.
Identification of a gene encoding a yeast histone H4 acetyltransferase.
Structure of the histone acetyltransferase Hat1: a paradigm for the GCN5-related N-acetyltransferase superfamily.
The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism.
The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly.
Type B histone acetyltransferase Hat1p participates in telomeric silencing.
Cloning and functional analysis of the arginyl-tRNA-protein transferase gene ATE1 of Saccharomyces cerevisiae.
The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance.
Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum.
Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity.
Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus.
The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis.
Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control.
Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor.
Elevation of endogenous sphingolipid long-chain base phosphates kills Saccharomyces cerevisiae cells.
Identification of the first mammalian sphingosine phosphate lyase gene and its functional expression in yeast.
Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids.
Identifying key residues of sphinganine-1-phosphate lyase for function in vivo and in vitro.
Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.
The BST1 gene of Saccharomyces cerevisiae is the sphingosine-1-phosphate lyase.
The DPL1 gene is involved in mediating the response to nutrient deprivation in Saccharomyces cerevisiae.
Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.
A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans.
Identification and functional expression of the mitochondrial pyruvate carrier.
Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation.
Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination.
Functional isolation of the Candida albicans FCR3 gene encoding a bZip transcription factor homologous to Saccharomyces cerevisiae Yap3p.
Isolation of a putative Candida albicans transcriptional regulator involved in pleiotropic drug resistance by functional complementation of a pdr1 pdr3 mutation in Saccharomyces cerevisiae.
Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p.
Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.
Differential effects of chromatin and Gcn4 on the 50-fold range of expression among individual yeast Ty1 retrotransposons.
Happy together: the life and times of Ty retrotransposons and their hosts.
Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence.
Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae.
Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae.
Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.
The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.
Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene.
The sequence of a 17,933 bp segment of Saccharomyces cerevisiae chromosome XIV contains the RHO2, TOP2, MKT1 and END3 genes and five new open reading frames.
Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as conserved interactors of BLOC-1.
The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin.
The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Interactions between three common subunits of yeast RNA polymerases I and III.
Phosphorylation of proteins by inositol pyrophosphates.
Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.
The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae chromosome XI contains the UBI2 and MPL1 genes and three new open reading frames.
BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae.
Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases.
The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex.
RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus.
Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags.
The study of methionine uptake in Saccharomyces cerevisiae reveals a new family of amino acid permeases.
A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase.
Regulation of the transcription factor Gcn4 by Pho85 cyclin PCL5.
Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE.
COQ2 is a candidate for the structural gene encoding para-hydroxybenzoate:polyprenyltransferase.
Coenzyme Q supplementation or over-expression of the yeast Coq8 putative kinase stabilizes multi-subunit Coq polypeptide complexes in yeast coq null mutants.
Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.
Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2.
Nucleotide degradation and ribose salvage in yeast.
The PGM3 gene encodes the major phosphoribomutase in the yeast Saccharomyces cerevisiae.
A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames.
IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein.
Sequence of a 10.27 kb segment on the left arm of chromosome XV from Saccharomyces cerevisiae includes part of the IRA2 gene and a putative new gene.
The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products.
Assessing systems properties of yeast mitochondria through an interaction map of the organelle.
Decarboxylation of sorbic acid by spoilage yeasts is associated with the PAD1 gene.
Distribution of phenolic yeasts and production of phenolic off-flavors in wine fermentation.
Effect of overexpression of Saccharomyces cerevisiae Pad1p on the resistance to phenylacrylic acids and lignocellulose hydrolysates under aerobic and oxygen-limited conditions.
Enhancing volatile phenol concentrations in wine by expressing various phenolic acid decarboxylase genes in Saccharomyces cerevisiae.
Genetic analysis of the metabolic pathways responsible for aroma metabolite production by Saccharomyces cerevisiae.
Identifying genes that impact on aroma profiles produced by Saccharomyces cerevisiae and the production of higher alcohols.
Overexpression of PAD1 and FDC1 results in significant cinnamic acid decarboxylase activity in Saccharomyces cerevisiae.
PAD1 encodes phenylacrylic acid decarboxylase which confers resistance to cinnamic acid in Saccharomyces cerevisiae.
Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae.
The role of UbiX in Escherichia coli coenzyme Q biosynthesis.
Architecture of initiation-competent 12-subunit RNA polymerase II.
Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage.
Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS.
Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.
Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model.
RNA polymerase II/TFIIF structure and conserved organization of the initiation complex.
Structural basis of transcription: RNA polymerase II at 2.8 A resolution.
Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution.
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.
Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center.
Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs.
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7.
Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III.
cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II.
Amino acid sequences of aspartate aminotransferases: the cytosolic isoenzymes from yeast and from human liver.
Aspartate: 2-oxoglutarate aminotransferase from bakers' yeast: crystallization and characterization.
Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase.
Cytosolic aspartate aminotransferase encoded by the AAT2 gene is targeted to the peroxisomes in oleate-grown Saccharomyces cerevisiae.
The amino acid sequence of the aspartate aminotransferase from baker's yeast (Saccharomyces cerevisiae).
The Saccharomyces cerevisiae TCM62 gene encodes a chaperone necessary for the assembly of the mitochondrial succinate dehydrogenase (complex II).
The chaperonin-related protein Tcm62p ensures mitochondrial gene expression under heat stress.
A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.
Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI.
Cell cycle-dependent assembly of a Gin4-septin complex.
Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins.
Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae.
Kcc4 associates with septin proteins of Saccharomyces cerevisiae.
Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function.
Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.
Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae.
SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells.
The carboxy-terminal tails of septins Cdc11 and Shs1 recruit myosin-II binding factor Bni5 to the bud neck in Saccharomyces cerevisiae.
The septins: roles in cytokinesis and other processes.
2.8-A structure of yeast serine carboxypeptidase.
A newly characterized vacuolar serine carboxypeptidase, Atg42/Ybr139w, is required for normal vacuole function and the terminal steps of autophagy in the yeast Saccharomyces cerevisiae.
Biosynthesis of the vacuolar yeast glycoprotein carboxypeptidase Y. Conversion of precursor into the enzyme.
Dissection of glutathione conjugate turnover in yeast.
High-level expression and characterization of carboxypeptidase Y from Saccharomyces cerevisiae in Pichia pastoris GS115.
Inactivation of carboxypeptidase Y by mutational removal of the putative essential histidyl residue.
N-glycosylation analysis of yeast carboxypeptidase Y reveals the ultimate removal of phosphate from glycans at Asn(368).
Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae.
Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae.
Protein sorting in yeast: the localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide.
Site-directed mutagenesis on (serine) carboxypeptidase Y. A hydrogen bond network stabilizes the transition state by interaction with the C-terminal carboxylate group of the substrate.
Structure of the carboxypeptidase Y inhibitor IC in complex with the cognate proteinase reveals a novel mode of the proteinase-protein inhibitor interaction.
Studies on the hydrolytic properties of (serine) carboxypeptidase Y.
Mnl2, a novel component of the ER associated protein degradation pathway.
Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae.
Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex.
Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity.
Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis.
Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock.
Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions.
Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae.
Engineering of Saccharomyces cerevisiae for the production of L-glycerol 3-phosphate.
Fermentative production of L-glycerol 3-phosphate utilizing a Saccharomyces cerevisiae strain with an engineered glycerol biosynthetic pathway.
Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses.
Identification of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionization mass spectrometry.
Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway.
Metabolic regulation rather than de novo enzyme synthesis dominates the osmo-adaptation of yeast.
Microaerobic glycerol formation in Saccharomyces cerevisiae.
Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis.
Protein identifications for a Saccharomyces cerevisiae protein database.
Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway.
The YIG1 (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae.
The control of intracellular glycerol in Saccharomyces cerevisiae influences osmotic stress response and resistance to increased temperature.
The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress.
Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7.
Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family.
Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex.
Mapping histone fold TAFs within yeast TFIID.
Molecular characterization of Saccharomyces cerevisiae TFIID.
Multi-protein complexes in eukaryotic gene transcription.
The histone fold is a key structural motif of transcription factor TFIID.
A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeast.
Methylation of translation-associated proteins in Saccharomyces cerevisiae: Identification of methylated lysines and their methyltransferases.
Yeast ribosomal/cytochrome c SET domain methyltransferase subfamily: identification of Rpl23ab methylation sites and recognition motifs.
A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast.
A superfamily of membrane-bound O-acyltransferases with implications for wnt signaling.
Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology.
Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
Expression studies of GUP1 and GUP2, genes involved in glycerol active transport in Saccharomyces cerevisiae, using semi-quantitative RT-PCR.
GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae.
GUP1 of Saccharomyces cerevisiae encodes an O-acyltransferase involved in remodeling of the GPI anchor.
Programmed cell death in Saccharomyces cerevisiae is hampered by the deletion of GUP1 gene.
Saccharomyces cerevisiae mitochondrial Por1/yVDAC1 (voltage-dependent anion channel 1) interacts physically with the MBOAT O-acyltransferase Gup1/HHATL in the control of cell wall integrity and programmed cell death.
Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII.
Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein.
The Gup1 homologue of Trypanosoma brucei is a GPI glycosylphosphatidylinositol remodelase.
The yeast O-acyltransferase Gup1p interferes in lipid metabolism with direct consequences on the sphingolipid-sterol-ordered domains integrity/assembly.
Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
Yeast orthologues associated with glycerol transport and metabolism.
Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2.
Quality control of inner nuclear membrane proteins by the Asi complex.
Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae.
Dissociable Rpb4-Rpb7 subassembly of RNA polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation.
Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.
RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability.
Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.
Structure and mechanism of RNA polymerase II CTD phosphatases.
The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms.
NudEL targets dynein to microtubule ends through LIS1.
Characterization of the Saccharomyces cerevisiae Fol1 protein: starvation for C1 carrier induces pseudohyphal growth.
Phosphorylation by casein kinase 2 regulates Nap1 localization and function.
Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae.
The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae.
Identification of a 60S preribosomal particle that is closely linked to nuclear export.
Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p.
Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis.
Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA.
Cyclophilin A and Ess1 interact with and regulate silencing by the Sin3-Rpd3 histone deacetylase.
Mutations in a peptidylprolyl-cis/trans-isomerase gene lead to a defect in 3'-end formation of a pre-mRNA in Saccharomyces cerevisiae.
PTF1 encodes an essential protein in Saccharomyces cerevisiae, which shows strong homology with a new putative family of PPIases.
Phospho-carboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA 3'-End formation.
Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone.
Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae.
Vanishingly low levels of Ess1 prolyl-isomerase activity are sufficient for growth in Saccharomyces cerevisiae.
A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames.
Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.
The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes catalyze a two-step reaction leading to a soluble LL-dityrosine-containing precursor of the yeast spore wall.
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.
A panoramic view of yeast noncoding RNA processing.
Regulation of dimorphism in Saccharomyces cerevisiae: involvement of the novel protein kinase homolog Elm1p and protein phosphatase 2A.
Specific inhibition of Elm1 kinase activity reveals functions required for early G1 events.
The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames.
Prp43: an RNA helicase-like factor involved in spliceosome disassembly.
Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.
Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.
COX16 encodes a novel protein required for the assembly of cytochrome oxidase in Saccharomyces cerevisiae.
Cox16 protein is physically associated with Cox1p assembly intermediates and with cytochrome oxidase.
Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI.
Structure and function of MRP20 and MRP49, the nuclear genes for two proteins of the 54 S subunit of the yeast mitochondrial ribosome.
A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondrial of Saccharomyces cerevisiae.
Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence.
Structure-function analysis of enoyl thioester reductase involved in mitochondrial maintenance.
The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene.
ZAP1-mediated modulation of triacylglycerol levels in yeast by transcriptional control of mitochondrial fatty acid biosynthesis.
A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions.
Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis.
Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV.
NMR study of Rcf2 reveals an unusual dimeric topology in detergent micelles.
Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex.
Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex.
Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1.
The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force.
Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis.
Crystal structures and catalytic mechanism of the C-methyltransferase Coq5 provide insights into a key step of the yeast coenzyme Q synthesis pathway.
The COQ5 gene encodes a yeast mitochondrial protein necessary for ubiquinone biosynthesis and the assembly of the respiratory chain.
Yeast Coq5 C-methyltransferase is required for stability of other polypeptides involved in coenzyme Q biosynthesis.
Activity and autophosphorylation of LAMMER protein kinases.
Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog.
The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family.
The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein.
A Sm-like protein complex that participates in mRNA degradation.
Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover.
Characterization of Sm-like proteins in yeast and their association with U6 snRNA.
Crystal structure and biochemical analysis of the heptameric Lsm1-7 complex.
Crystal structure of Lsm3 octamer from Saccharomyces cerevisiae: implications for Lsm ring organisation and recruitment.
Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA.
Functional analysis of the regulatory region adjacent to the cargB gene of Saccharomyces cerevisiae. Nucleotide sequence, gene fusion experiments and cis-dominant regulatory mutation analysis.
Gene MRP-L4, encoding mitochondrial ribosomal protein YmL4, is indispensable for proper non-respiratory cell functions in yeast.
Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.
Lsm Proteins are required for normal processing and stability of ribosomal RNAs.
Lsm proteins are required for normal processing of pre-tRNAs and their efficient association with La-homologous protein Lhp1p.
Lsm2 and Lsm3 bridge the interaction of the Lsm1-7 complex with Pat1 for decapping activation.
Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs.
Yeast Sm-like proteins function in mRNA decapping and decay.
A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protection.
Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae.
Aft1p and Aft2p mediate iron-responsive gene expression in yeast through related promoter elements.
Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.
NAD+-dependent deacetylase Hst1p controls biosynthesis and cellular NAD+ levels in Saccharomyces cerevisiae.
The FOX transcription factor Hcm1 regulates oxidative metabolism in response to early nutrient limitation in yeast. Role of Snf1 and Tor1/Sch9 kinases.
Conservation of structure and function among histidine-containing phosphotransfer (HPt) domains as revealed by the crystal structure of YPD1.
Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae.
Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system.
Novel role for an HPt domain in stabilizing the phosphorylated state of a response regulator domain.
Pradimicin-resistance of yeast is caused by a point mutation of the histidine-containing phosphotransfer protein Ypd1.
Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of Ssk1p and Skn7p.
The yeast YPD1/SLN1 complex: insights into molecular recognition in two-component signaling systems.
The yeast histidine protein kinase, Sln1p, mediates phosphotransfer to two response regulators, Ssk1p and Skn7p.
Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 two-component osmosensor.
A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP.
A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution.
Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction.
Genome-wide analysis of membrane targeting by S.cerevisiae pleckstrin homology domains.
Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues.
Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein-related proteins and phosphoinositides.
Osh proteins regulate COPII-mediated vesicular transport of ceramide from the endoplasmic reticulum in budding yeast.
Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sites.
Overlapping functions of the yeast oxysterol-binding protein homologues.
Structure of Osh3 reveals a conserved mode of phosphoinositide binding in oxysterol-binding proteins.
The GOLD domain, a novel protein module involved in Golgi function and secretion.
Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock.
Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.
Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification.
High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing.
Incomplete arrest in the outer membrane sorts NADH-cytochrome b5 reductase to two different submitochondrial compartments.
Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae.
Intermembrane space proteome of yeast mitochondria.
Mitochondrial NADH-cytochrome b(5) reductase plays a crucial role in the reduction of D-erythroascorbyl free radical in Saccharomyces cerevisiae.
Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins.
The yeast mitochondrial intermembrane space: purification and analysis of two distinct fractions.
Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues.
Multiple motif scanning to identify methyltransferases from the yeast proteome.
Probabilistic approach to predicting substrate specificity of methyltransferases.
The structure of the yeast mitochondrial ribosome.
Histone H3 specific acetyltransferases are essential for cell cycle progression.
Methylation of histone H3 mediates the association of the NuA3 histone acetyltransferase with chromatin.
Sas3 is a histone acetyltransferase and requires a zinc finger motif.
Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes.
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
Yeast SAS silencing genes and human genes associated with AML and HIV-1 Tat interactions are homologous with acetyltransferases.
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis.
Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae.
RNA methylation by the MIS complex regulates a cell fate decision in yeast.
Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae.
Vir1p, the yeast homolog of virilizer, is required for mRNA m6A methylation and meiosis.
Analysis of the genes activated by the FLO8 gene in Saccharomyces cerevisiae.
The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae.
AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases.
Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1.
Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation.
Structural and enzymatic properties of the AAA protein Drg1p from Saccharomyces cerevisiae. Decoupling of intracellular function from ATPase activity and hexamerization.
The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1.
Crystal structure of Saccharomyces cerevisiae cytoplasmic thioredoxin reductase Trr1 reveals the structural basis for species-specific recognition of thioredoxin.
Insights into the specificity of thioredoxin reductase-thioredoxin interactions. A structural and functional investigation of the yeast thioredoxin system.
The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
Thioredoxin-dependent peroxide reductase from yeast.
Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins.
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site.
Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.
Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region.
The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis.
Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae.
1H, 13C and 15N resonance assignments and secondary structure of ADR6 DNA-binding domain.
A small, glutamine-free domain propagates the [SWI(+)] prion in budding yeast.
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
Distinct subregions of Swi1 manifest striking differences in prion transmission and SWI/SNF function.
Newly identified prion linked to the chromatin-remodeling factor Swi1 in Saccharomyces cerevisiae.
Structural analysis of the yeast SWI/SNF chromatin remodeling complex.
The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain.
[SWI], the prion formed by the chromatin remodeling factor Swi1, is highly sensitive to alterations in Hsp70 chaperone system activity.
Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p.
Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating.
The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs.
The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae.
A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides.
A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
Chemotyping of yeast mutants using robotics.
New weakly expressed cell cycle-regulated genes in yeast.
Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII.
Differential biochemical regulation of the URA7- and URA8-encoded CTP synthetases from Saccharomyces cerevisiae.
Effect of CTP synthetase regulation by CTP on phospholipid synthesis in Saccharomyces cerevisiae.
Use of synthetic lethal mutants to clone and characterize a novel CTP synthetase gene in Saccharomyces cerevisiae.
A high-yield co-expression system for the purification of an intact Drs2p-Cdc50p lipid flippase complex, critically dependent on and stabilized by phosphatidylinositol-4-phosphate.
Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail.
Autoinhibition and activation mechanisms of the eukaryotic lipid flippase Drs2p-Cdc50p.
Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.
Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae.
DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae.
Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function.
Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo.
Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis.
High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions.
Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases.
Isolation and characterization of novel mutations in CDC50, the non-catalytic subunit of the Drs2p phospholipid flippase.
Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles.
Mapping functional interactions in a heterodimeric phospholipid pump.
Reconstitution of phospholipid translocase activity with purified Drs2p, a type-IV P-type ATPase from budding yeast.
Regulation of a Golgi flippase by phosphoinositides and an ArfGEF.
Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function.
Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane.
Structural Basis of Substrate-Independent Phosphorylation in a P4-ATPase Lipid Flippase.
Structure and autoregulation of a P4-ATPase lipid flippase.
The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae.
The Essential Neo1 Protein from Budding Yeast Plays a Role in Establishing Aminophospholipid Asymmetry of the Plasma Membrane.
The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry.
Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases.
7-Methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (dcpS) enzymes and potently inhibits their activity.
Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis.
DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway.
Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies.
Functional analysis of mRNA scavenger decapping enzymes.
Scavenger decapping activity facilitates 5' to 3' mRNA decay.
The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast.
The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases.
Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI.
Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C).
Role of a ubiquitin-like modification in polarized morphogenesis.
Solution structure of the yeast ubiquitin-like modifier protein Hub1.
Investigating the protein-protein interactions of the yeast Hsp90 chaperone system by two-hybrid analysis: potential uses and limitations of this approach.
Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis.
The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface.
Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism.
MEI4, a meiosis-specific yeast gene required for chromosome synapsis.
Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation.
The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Characterization of AMD, the AMP deaminase gene in yeast. Production of amd strain, cloning, nucleotide sequence, and properties of the protein.
A lysosomal aminopeptidase isozyme in differentiating yeast cells and protoplasts.
A new class of mutants deficient in dodecamerization of aminopeptidase 1 and vacuolar transport.
Aminopeptidase I is targeted to the vacuole by a nonclassical vesicular mechanism.
Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway.
Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway.
Chloride as allosteric effector of yeast aminopeptidase I.
Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway.
Cvt19 is a receptor for the cytoplasm-to-vacuole targeting pathway.
Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole.
Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I.
Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae.
Metal binding to yeast aminopeptidase I.
Molecular cloning and sequencing of genomic DNA encoding aminopeptidase I from Saccharomyces cerevisiae.
Propeptide of aminopeptidase 1 protein mediates aggregation and vesicle formation in cytoplasm-to-vacuole targeting pathway.
Proteinases, proteolysis and biological control in the yeast Saccharomyces cerevisiae.
Subcellular localization and levels of aminopeptidases and dipeptidase in Saccharomyces cerevisiae.
The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast.
Transport of a large oligomeric protein by the cytoplasm to vacuole protein targeting pathway.
Two distinct pathways for targeting proteins from the cytoplasm to the vacuole/lysosome.
Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension.
Yeast aminopeptidase I. Chemical composition and catalytic properties.
Yeast vacuolar aminopeptidase yscI. Isolation and regulation of the APE1 (LAP4) structural gene.
Yol082p, a novel CVT protein involved in the selective targeting of aminopeptidase I to the yeast vacuole.
Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae.
Multicopy STS1 restores both protein transport and ribosomal RNA stability in a new yeast sec23 mutant allele.
Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX.
SQT1, which encodes an essential WD domain protein of Saccharomyces cerevisiae, suppresses dominant-negative mutations of the ribosomal protein gene QSR1.
A global protein kinase and phosphatase interaction network in yeast.
Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex.
Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy.
Structural and functional insights into Saccharomyces cerevisiae Tpa1, a putative prolylhydroxylase influencing translation termination and transcription.
Structure of the ribosomal oxygenase OGFOD1 provides insights into the regio- and stereoselectivity of prolyl hydroxylases.
Tpa1p is part of an mRNP complex that influences translation termination, mRNA deadenylation, and mRNA turnover in Saccharomyces cerevisiae.
Molecular analysis of the yeast VPS3 gene and the role of its product in vacuolar protein sorting and vacuolar segregation during the cell cycle.
Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.
Evidence that the pre-mRNA splicing factor Clf1p plays a role in DNA replication in Saccharomyces cerevisiae.
Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae.
Functional and physical interactions between components of the Prp19p-associated complex.
Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae.
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.
Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found.
The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts.
Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition.
A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits.
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes.
ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes.
Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body.
Structure of the ESCRT-II endosomal trafficking complex.
Ubiquitin interactions of NZF zinc fingers.
S-adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase.
The arginine methyltransferase Rmt2 is enriched in the nucleus and co-purifies with the nuclear porins Nup49, Nup57 and Nup100.
Yeast ribosomal protein L12 is a substrate of protein-arginine methyltransferase 2.
Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae.
DNA sequencing and analysis of 130 kb from yeast chromosome XV.
High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis.
Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast.
The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting.
Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition.
VPS21 controls entry of endocytosed and biosynthetic proteins into the yeast prevacuolar compartment.
VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins.
Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome.
Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport.
Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p.
Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae.
Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase. Expression and characterization of the enzyme.
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway.
ECA39 is regulated by c-Myc in human and by a Jun/Fos homolog, GCN4, in yeast.
Mitochondrial and cytosolic branched-chain amino acid transaminases from yeast, homologs of the myc oncogene-regulated Eca39 protein.
Saccharomyces cerevisiae Bat1 and Bat2 aminotransferases have functionally diverged from the ancestral-like Kluyveromyces lactis orthologous enzyme.
Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases.
Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.
Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.
New regulators of a high affinity Ca2+ influx system revealed through a genome-wide screen in yeast.
A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway.
An integrated approach for finding overlooked genes in yeast.
Enrichment-based proteogenomics identifies microproteins, missing proteins, and novel smORFs in Saccharomyces cerevisiae.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
A novel pathway of ceramide metabolism in Saccharomyces cerevisiae.
Compartmentalized synthesis of triacylglycerol at the inner nuclear membrane regulates nuclear organization.
Expanding of phospholipid:diacylglycerol acyltransferase (PDAT) from Saccharomyces cerevisiae as multifunctional biocatalyst with broad acyl donor/acceptor selectivity.
Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants.
Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities.
Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis.
The topology of the triacylglycerol synthesizing enzyme Lro1 indicates that neutral lipids can be produced within the luminal compartment of the endoplasmatic reticulum: Implications for the biogenesis of lipid droplets.
The ubiquitin-like (UBX)-domain-containing protein Ubx2/Ubxd8 regulates lipid droplet homeostasis.
Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm.
Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis.
Functional characterization of the INO2 gene of Saccharomyces cerevisiae. A positive regulator of phospholipid biosynthesis.
Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae.
The INO2 gene of Saccharomyces cerevisiae encodes a helix-loop-helix protein that is required for activation of phospholipid synthesis.
ADY1, a novel gene required for prospore membrane formation at selected spindle poles in Saccharomyces cerevisiae.
Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae.
Prospore membrane formation linked to the leading edge protein (LEP) coat assembly.
Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis.
Systematic discovery of new genes in the Saccharomyces cerevisiae genome.
Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued.
Arp10p is a pointed-end-associated component of yeast dynactin.
Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae.
The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle.
The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B.
Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae.
Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex.
Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon.
Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae.
Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X.
Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase.
Competitive promoter occupancy by two yeast paralogous transcription factors controlling the multidrug resistance phenomenon.
Lpx1p is a peroxisomal lipase required for normal peroxisome morphology.
Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae.
Transcriptome profiling to identify genes involved in peroxisome assembly and function.
Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins.
ATP hydrolysis is required for cohesin's association with chromosomes.
Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins.
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
Phosphorylation of the Scc2 cohesin deposition complex subunit regulates chromosome condensation through cohesin integrity.
Structural evidence for Scc4-dependent localization of cohesin loading.
The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions.
Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication.
Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex.
Chromatin remodeling by RSC involves ATP-dependent DNA translocation.
Histone octamer transfer by a chromatin-remodeling complex.
RSC, an essential, abundant chromatin-remodeling complex.
The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles.
The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling.
The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation.
Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC.
Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF.
Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains.
Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family.
Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway.
The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic.
The binary interacting network of the conserved oligomeric Golgi tethering complex.
The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae.
Epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP.
Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast.
Characterization of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TID1, in mitosis and meiosis.
Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway.
DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway.
Finding functional features in Saccharomyces genomes by phylogenetic footprinting.
Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1.
RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis.
Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II.
The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break.
Tid1/Rdh54 promotes colocalization of Rad51 and Dmc1 during meiotic recombination.
Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I.
The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1-->6)-beta-D-glucan synthesis and normal cell growth.
Identification of the Saccharomyces cerevisiae genes STB1-STB5 encoding Sin3p binding proteins.
Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p.
KAR3, a kinesin-related gene required for yeast nuclear fusion.
Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations.
The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae.
X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution.
Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.
Gpi19, the Saccharomyces cerevisiae homologue of mammalian PIG-P, is a subunit of the initial enzyme for glycosylphosphatidylinositol anchor biosynthesis.
A block to mRNA nuclear export in S. cerevisiae leads to hyperadenylation of transcripts that accumulate at the site of transcription.
An RNA-export mediator with an essential nuclear export signal.
Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function.
Peering through the pore: nuclear pore complex structure, assembly, and function.
Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells.
The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p.
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
A family of yeast proteins mediating bidirectional vacuolar amino acid transport.
Ubp15p, a ubiquitin hydrolase associated with the peroxisomal export machinery.
A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism.
Crystal Structure of Saccharomyces cerevisiae ECM4, a Xi-Class Glutathione Transferase that Reacts with Glutathionyl-(hydro)quinones.
Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.
Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases.
DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43.
Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes.
A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator.
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
Analysis of the interaction between piD261/Bud32, an evolutionarily conserved protein kinase of Saccharomyces cerevisiae, and the Grx4 glutaredoxin.
Gcn4 misregulation reveals a direct role for the evolutionary conserved EKC/KEOPS in the t6A modification of tRNAs.
In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya.
Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system.
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
Yeast homolog of a cancer-testis antigen defines a new transcription complex.
Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae.
Deletion of EFL1 results in heterogeneity of the 60 S GTPase-associated rRNA conformation.
Ria1p (Ynl163c), a protein similar to elongation factors 2, is involved in the biogenesis of the 60S subunit of the ribosome in Saccharomyces cerevisiae.
The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis.
The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase.
Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames.
Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex.
Membrane topology of the endoplasmic reticulum to Golgi transport factor Erv29p.
Role of Erv29p in collecting soluble secretory proteins into ER-derived transport vesicles.
Sorting signals can direct receptor-mediated export of soluble proteins into COPII vesicles.
Characterization of the SAC3 gene of Saccharomyces cerevisiae.
Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism.
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery.
The Saccharomyces cerevisiae LEP1/SAC3 gene is associated with leucine transport.
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery.
Molecular cloning and characterization of a full-length flavin-dependent monooxygenase from yeast.
A Ser/Thr-rich multicopy suppressor of a cdc24 bud emergence defect.
A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast.
A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch.
Mitogen-activated protein kinases with distinct requirements for Ste5 scaffolding influence signaling specificity in Saccharomyces cerevisiae.
Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1.
The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response.
Effects of mannoprotein mutations on Saccharomyces cerevisiae core oligosaccharide structure.
Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae.
Saccharomyces cerevisiae mannoprotein mutants. Isolation of the mnn5 mutant and comparison with the mnn3 strain.
Svp26 facilitates endoplasmic reticulum to Golgi transport of a set of mannosyltransferases in Saccharomyces cerevisiae.
Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV.
Genome-wide expression analysis of NAP1 in Saccharomyces cerevisiae.
Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus.
Members of the NAP/SET family of proteins interact specifically with B-type cyclins.
A novel membrane protein complex on the endoplasmic reticulum and early Golgi compartments in the yeast Saccharomyces cerevisiae.
Immunoisolation of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments.
The Erv41p-Erv46p complex: multiple export signals are required in trans for COPII-dependent transport from the ER.
Cryo-EM structure of the yeast respiratory supercomplex.
Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c.
Sequencing of the nuclear gene for the yeast cytochrome c1 precursor reveals an unusually complex amino-terminal presequence.
Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment.
Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer.
Supercomplexes in the respiratory chains of yeast and mammalian mitochondria.
The cytochrome bc1 and cytochrome c oxidase complexes associate to form a single supracomplex in yeast mitochondria.
The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames.
Two distinct and independent mitochondrial targeting signals function in the sorting of an inner membrane protein, cytochrome c1.
The SPS4 gene of Saccharomyces cerevisiae encodes a major sporulation-specific mRNA.
A gated channel into the proteasome core particle.
Crystal structure of the boronic acid-based proteasome inhibitor bortezomib in complex with the yeast 20S proteasome.
Crystal structures of salinosporamide A (NPI-0052) and B (NPI-0047) in complex with the 20S proteasome reveal important consequences of beta-lactone ring opening and a mechanism for irreversible binding.
Near-atomic resolution structural model of the yeast 26S proteasome.
Nucleotide sequence of PUP1 encoding a putative proteasome subunit in Saccharomyces cerevisiae.
Structural basis for the activation of 20S proteasomes by 11S regulators.
Structure of 20S proteasome from yeast at 2.4-A resolution.
Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening.
TMC-95-based inhibitor design provides evidence for the catalytic versatility of the proteasome.
Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae.
The role of Saccharomyces cerevisiae Met1p and Met8p in sirohaem and cobalamin biosynthesis.
The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.
A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg).
Saccharomyces cerevisiae contains a Type II phosphoinositide 4-kinase.
The Saccharomyces cerevisiae LSB6 gene encodes phosphatidylinositol 4-kinase activity.
'Prion-proof' for [PIN+]: infection with in vitro-made amyloid aggregates of Rnq1p-(132-405) induces [PIN+].
Amyloid of Rnq1p, the basis of the [PIN+] prion, has a parallel in-register beta-sheet structure.
Genetic and environmental factors affecting the de novo appearance of the [PSI+] prion in Saccharomyces cerevisiae.
Prion protein gene polymorphisms in Saccharomyces cerevisiae.
Prions affect the appearance of other prions: the story of [PIN(+)].
Rnq1: an epigenetic modifier of protein function in yeast.
The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames.
Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
A survey of essential gene function in the yeast cell division cycle.
NQR1 controls lifespan by regulating the promotion of respiratory metabolism in yeast.
Binding of the Fkh1 forkhead associated domain to a phosphopeptide within the Mph1 DNA helicase regulates mating-type switching in budding yeast.
Cell cycle-dependent regulation of Saccharomyces cerevisiae donor preference during mating-type switching by SBF (Swi4/Swi6) and Fkh1.
Fkh1 and Fkh2 bind multiple chromosomal elements in the S. cerevisiae genome with distinct specificities and cell cycle dynamics.
Forkhead genes in transcriptional silencing, cell morphology and the cell cycle. Overlapping and distinct functions for FKH1 and FKH2 in Saccharomyces cerevisiae.
Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae.
Forkhead transcription factors, Fkh1p and Fkh2p, collaborate with Mcm1p to control transcription required for M-phase.
Forkhead-like transcription factors recruit Ndd1 to the chromatin of G2/M-specific promoters.
Mechanisms controlling differential promoter-occupancy by the yeast forkhead proteins Fkh1p and Fkh2p: implications for regulating the cell cycle and differentiation.
Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase.
Regulation of budding yeast mating-type switching donor preference by the FHA domain of Fkh1.
Regulation of elongating RNA polymerase II by forkhead transcription factors in yeast.
Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer.
The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2.
The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.
The yeast forkhead transcription factors fkh1 and fkh2 regulate lifespan and stress response together with the anaphase-promoting complex.
Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth.
A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p.
Isolation and characterization of a thiamin transport gene, THI10, from Saccharomyces cerevisiae.
Avl9p, a member of a novel protein superfamily, functions in the late secretory pathway.
Global analysis of protein palmitoylation in yeast.
High-confidence proteomic analysis of yeast lipid droplets identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation.
Identification of DRG family regulatory proteins (DFRPs): specific regulation of DRG1 and DRG2.
Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1.
Identification of the mitochondrial NAD+ transporter in Saccharomyces cerevisiae.
Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiae.
MCART1/SLC25A51 is required for mitochondrial NAD transport.
SLC25A51 is a mammalian mitochondrial NAD+ transporter.
The crystal structure and identification of NQM1/YGR043C, a transaldolase from Saccharomyces cerevisiae.
Sequence of a 9.8 kb segment of yeast chromosome II including the three genes of the MAL3 locus and three unidentified open reading frames.
AFG1, a new member of the SEC18-NSF, PAS1, CDC48-VCP, TBP family of ATPases.
A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box.
Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae.
The upstream activation site of a Ty2 element of yeast is necessary but not sufficient to promote maximal transcription of the element.
Pls1 Is a Peroxisomal Matrix Protein with a Role in Regulating Lysine Biosynthesis.
Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins.
The sequence of 12.8 kb from the left arm of chromosome XIV reveals a sigma element, a pro-tRNA and six complete open reading frames, one of which encodes a protein similar to the human leukotriene A4 hydrolase.
Crystal structure of the worm NitFhit Rosetta stone protein reveals a Nit tetramer binding two Fhit dimers.
Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione.
Structures of enzyme-intermediate complexes of yeast Nit2: insights into its catalytic mechanism and different substrate specificity compared with mammalian Nit2.
IBD2 encodes a novel component of the Bub2p-dependent spindle checkpoint in the budding yeast Saccharomyces cerevisiae.
Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs.
The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism.
The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities.
Sequence analysis of the right end of chromosome XV in Saccharomyces cerevisiae: an insight into the structural and functional significance of sub-telomeric repeat sequences.
The structure and evolution of subtelomeric Y' repeats in Saccharomyces cerevisiae.
Cysteinyl-tRNA synthetase from Saccharomyces cerevisiae. Purification, characterization and assignment to the genomic sequence YNL247w.
A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters.
Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.
ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin.
BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae.
Internal targeting signal of the BCS1 protein: a novel mechanism of import into mitochondria.
A proteomics approach to understanding protein ubiquitination.
ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose.
Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription.
The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation.
A novel Nudix hydrolase for oxidized purine nucleoside triphosphates encoded by ORFYLR151c (PCD1 gene) in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae PCD1 gene encodes a peroxisomal nudix hydrolase active toward coenzyme A and its derivatives.
Analysis of the vacuolar luminal proteome of Saccharomyces cerevisiae.
Aspartyl aminopeptidase is imported from the cytoplasm to the vacuole by selective autophagy in Saccharomyces cerevisiae.
Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells.
Subcellular localization of the yeast proteome.
2.4 A resolution crystal structure of the prototypical hormone-processing protease Kex2 in complex with an Ala-Lys-Arg boronic acid inhibitor.
Posttranslational processing of the prohormone-cleaving Kex2 protease in the Saccharomyces cerevisiae secretory pathway.
Structural and enzymatic characterization of a purified prohormone-processing enzyme: secreted, soluble Kex2 protease.
The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain.
The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing.
Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases.
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback.
Mitochondrial translational-initiation and elongation factors in Saccharomyces cerevisiae.
Prediction and identification of new natural substrates of the yeast mitochondrial intermediate peptidase.
Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae.
Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.
A yeast phosphofructokinase insensitive to the Fructose 2,6-bisphosphate; allosteric activator. Glycolysis/metabolic regulation/allosteric control.
Activation by phosphate of yeast phosphofructokinase.
An electron microscopy study of the quarternary structure of yeast phosphofructokinase.
Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method.
Enolase takes part in a macromolecular complex associated to mitochondria in yeast.
Glycolysis mutants in Saccharomyces cerevisiae.
Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast.
Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases.
Mutant studies of yeast phosphofructokinase.
Physicochemical parameters and subunit composition of yeast phosphofructokinase.
Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII.
Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII.
Similarity of activation of yeast phosphofructokinase by AMP and fructose-2,6-bisphosphate.
Single point mutations in either gene encoding the subunits of the heterooctameric yeast phosphofructokinase abolish allosteric inhibition by ATP.
Stimulation of yeast phosphofructokinase activity by fructose 2,6-bisphosphate.
Studies on the function of yeast phosphofructokinase subunits by in vitro mutagenesis.
Temporal organization of the phosphofructokinase/fructose-1,6-biphosphatase cycle.
The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle.
The phosphofructokinase genes of yeast evolved from two duplication events.
Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: ethanolamine-dependent effects on steady-state membrane phospholipid composition in Saccharomyces cerevisiae.
Phosphatidylcholine biosynthesis in Saccharomyces cerevisiae. Regulatory insights from studies employing null and chimeric sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases.
Studies employing Saccharomyces cerevisiae cpt1 and ept1 null mutants implicate the CPT1 gene in coordinate regulation of phospholipid biosynthesis.
The selective utilization of substrates in vivo by the phosphatidylethanolamine and phosphatidylcholine biosynthetic enzymes Ept1p and Cpt1p in yeast.
The sn-1,2-diacylglycerol ethanolaminephosphotransferase activity of Saccharomyces cerevisiae. Isolation of mutants and cloning of the EPT1 gene.
sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Mixed micellar analysis of the CPT1 and EPT1 gene products.
sn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Nucleotide sequence of the EPT1 gene and comparison of the CPT1 and EPT1 gene products.
Genetic identification of factors that modulate ribosomal DNA transcription in Saccharomyces cerevisiae.
The nucleolar antigen Nop52, the human homologue of the yeast ribosomal RNA processing RRP1, is recruited at late stages of nucleologenesis.
The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum.
A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAS.
Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.
Different substrate specificities of lanosterol 14a-demethylase (P-45014DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol.
Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex.
Involvement of cytochrome P-450 and a cyanide-sensitive enzyme in different steps of lanosterol demethylation by yeast microsomes.
Isolation of a cytochrome P-450 structural gene from Saccharomyces cerevisiae.
Metabolism of 32-hydroxy-24,25-dihydrolanosterol by purified cytochrome P-45014DM from yeast. Evidence for contribution of the cytochrome to whole process of lanosterol 14 alpha-demethylation.
Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14 alpha-demethylase (ERG11).
Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae.
Regulation of ergosterol biosynthesis in Saccharomyces cerevisiae.
The 14alpha-demethylation of lanosterol by a reconstituted cytochrome P-450 system from yeast microsomes.
A mutation in the MSH6 subunit of the Saccharomyces cerevisiae MSH2-MSH6 complex disrupts mismatch recognition.
ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes.
Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system.
Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.
Analysis of yeast MSH2-MSH6 suggests that the initiation of mismatch repair can be separated into discrete steps.
Asymmetric recognition of DNA local distortion. Structure-based functional studies of eukaryotic Msh2-Msh6.
Biochemical basis for dominant mutations in the Saccharomyces cerevisiae MSH6 gene.
Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.
Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.
Cadmium inhibits the functions of eukaryotic MutS complexes.
Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.
Contribution of Msh2 and Msh6 subunits to the asymmetric ATPase and DNA mismatch binding activities of Saccharomyces cerevisiae Msh2-Msh6 mismatch repair protein.
Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis.
Dominant Saccharomyces cerevisiae msh6 mutations cause increased mispair binding and decreased dissociation from mispairs by Msh2-Msh6 in the presence of ATP.
Evidence for sequential action of two ATPase active sites in yeast Msh2-Msh6.
Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes.
Germ-line msh6 mutations in colorectal cancer families.
Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2(ATP)-Msh6(ATP) state capable of hydrolysis-independent movement along DNA.
MSH-MLH complexes formed at a DNA mismatch are disrupted by the PCNA sliding clamp.
MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae.
MSH6, a Saccharomyces cerevisiae protein that binds to mismatches as a heterodimer with MSH2.
Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.
Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.
Multiple functions for the N-terminal region of Msh6.
Novel dominant mutations in Saccharomyces cerevisiae MSH6.
Proliferating cell nuclear antigen and Msh2p-Msh6p interact to form an active mispair recognition complex.
Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.
Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition.
Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes.
Saccharomyces cerevisiae Msh2p and Msh6p ATPase activities are both required during mismatch repair.
Specialized mismatch repair function of Glu339 in the Phe-X-Glu motif of yeast Msh6.
The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.
The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs.
Transfer of the MSH2.MSH6 complex from proliferating cell nuclear antigen to mispaired bases in DNA.
Characterization of a Saccharomyces cerevisiae gene that encodes a mitochondrial phosphate transporter-like protein.
Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana.
Isolation and characterization of a Saccharomyces cerevisiae peptide transport gene.
Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli.
Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase.
Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene.
Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae.
The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication.
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae.
A multiprotein complex that interacts with RNA polymerase II elongator.
An early step in wobble uridine tRNA modification requires the Elongator complex.
Architecture of the yeast Elongator complex.
Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae.
Crystal structure of elongator subcomplex Elp4-6.
Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo.
Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.
Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation.
Molecular architecture of the yeast Elongator complex reveals an unexpected asymmetric subunit arrangement.
Molecular basis of tRNA recognition by the Elongator complex.
RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes.
Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin.
Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora.
Structural insights into the function of Elongator.
The Elongator subcomplex Elp456 is a hexameric RecA-like ATPase.
Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis.
Haploid genetic screens in human cells identify host factors used by pathogens.
The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.
Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway.
YBR246W is required for the third step of diphthamide biosynthesis.
A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast.
Activation and processing of non-anchored yapsin 1 (Yap3p).
Activation mechanism, functional role and shedding of glycosylphosphatidylinositol-anchored Yps1p at the Saccharomyces cerevisiae cell surface.
Cleavage efficiency of the novel aspartic protease yapsin 1 (Yap3p) enhanced for substrates with arginine residues flanking the P1 site: correlation with electronegative active-site pockets predicted by molecular modeling.
Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast.
Proteolytic function of GPI-anchored plasma membrane protease Yps1p in the yeast vacuole and Golgi.
Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme.
Secretion of yeast aspartic protease 3 is regulated by its carboxy-terminal tail: characterization of secreted YAP3p.
The omega-site sequence of glycosylphosphatidylinositol-anchored proteins in Saccharomyces cerevisiae can determine distribution between the membrane and the cell wall.
The yeast proprotein convertase encoded by YAP3 is a glycophosphatidylinositol-anchored protein that localizes to the plasma membrane.
Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.
A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast.
Comparative biochemical studies of the murine fatty acid transport proteins (FATP) expressed in yeast.
Disruption of a yeast very-long-chain acyl-CoA synthetase gene simulates the cellular phenotype of X-linked adrenoleukodystrophy.
Disruption of the Saccharomyces cerevisiae FAT1 gene decreases very long-chain fatty acyl-CoA synthetase activity and elevates intracellular very long-chain fatty acid concentrations.
Disruption of the Saccharomyces cerevisiae homologue to the murine fatty acid transport protein impairs uptake and growth on long-chain fatty acids.
Fatty acid transport in Saccharomyces cerevisiae. Directed mutagenesis of FAT1 distinguishes the biochemical activities associated with Fat1p.
Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae.
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome.
Peroxisomal fatty acid uptake mechanism in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels.
Topology of the yeast fatty acid transport protein Fat1p: mechanistic implications for functional domains on the cytosolic surface of the plasma membrane.
Vectorial acylation in Saccharomyces cerevisiae. Fat1p and fatty acyl-CoA synthetase are interacting components of a fatty acid import complex.
Identification and characterization of SNQ2, a new multidrug ATP binding cassette transporter of the yeast plasma membrane.
Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast.
5' untranslated sequences are required for the translational control of a yeast regulatory gene.
A GCN4 protein recognition element is not sufficient for GCN4-dependent regulation of transcription in the ARO7 promoter of Saccharomyces cerevisiae.
Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo.
Amino acid-dependent Gcn4p stability regulation occurs exclusively in the yeast nucleus.
Construction of dimeric F(ab) useful in blood group serology.
Crystal structure of GCN4-pIQI, a trimeric coiled coil with buried polar residues.
Deconstruction of GCN4/GCRE into a monomeric peptide-DNA complex.
Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny.
Evidence for translational regulation of the activator of general amino acid control in yeast.
Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast.
GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA.
Gcn4 Binding in Coding Regions Can Activate Internal and Canonical 5' Promoters in Yeast.
Glucose limitation induces GCN4 translation by activation of Gcn2 protein kinase.
Monitoring the Gcn4 protein-mediated response in the yeast Saccharomyces cerevisiae.
Multiple upstream AUG codons mediate translational control of GCN4.
Mutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sites.
Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p.
Saturation mutagenesis of the yeast his3 regulatory site: requirements for transcriptional induction and for binding by GCN4 activator protein.
Sequences that surround the stop codons of upstream open reading frames in GCN4 mRNA determine their distinct functions in translational control.
Suppression of ribosomal reinitiation at upstream open reading frames in amino acid-starved cells forms the basis for GCN4 translational control.
The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted alpha helices: crystal structure of the protein-DNA complex.
The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complex.
The solution structure of a leucine-zipper motif peptide.
The transcriptional activator GCN4 contains multiple activation domains that are critically dependent on hydrophobic amino acids.
Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator.
Transcriptional activation of yeast nucleotide biosynthetic gene ADE4 by GCN4.
Transcriptional profiling shows that Gcn4p is a master regulator of gene expression during amino acid starvation in yeast.
Translation of the yeast transcriptional activator GCN4 is stimulated by purine limitation: implications for activation of the protein kinase GCN2.
X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil.
A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae.
Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit.
Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme.
Nucleocytoplasmic distribution of budding yeast protein kinase A regulatory subunit Bcy1 requires Zds1 and is regulated by Yak1-dependent phosphorylation of its targeting domain.
Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export.
Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant.
The Saccharomyces cerevisiae SSD1 gene is involved in the tolerance to high concentration of Ca2+ with the participation of HST1/NRC1/BFR1.
ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae.
Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae.
The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway.
KTR2: a new member of the KRE2 mannosyltransferase gene family.
Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly.
A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis.
Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA.
Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing.
A hypoxic consensus operator and a constitutive activation region regulate the ANB1 gene of Saccharomyces cerevisiae.
Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases.
Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae.
Hypusine formation in eukaryotic initiation factor 4D is not reversed when rates or specificity of protein synthesis is altered.
Hypusine-containing protein eIF5A promotes translation elongation.
Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae.
Identification of an upstream repressor site controlling the expression of an anaerobic gene (ANB1) in Saccharomyces cerevisiae.
Negative regulation of the Saccharomyces cerevisiae ANB1 gene by heme, as mediated by the ROX1 gene product.
ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae.
The ANB1 locus of Saccharomyces cerevisiae encodes the protein synthesis initiation factor eIF-4D.
The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.
Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae.
Translation initiation factor eIF-5A expressed from either of two yeast genes or from human cDNA. Functional identity under aerobic and anaerobic conditions.
eIF5A has a function in the elongation step of translation in yeast.
A complex of Pdi1p and the mannosidase Htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum.
Determination of the sequence of the yeast YCL313 gene localized on chromosome III. Homology with the protein disulfide isomerase (PDI gene product) of other organisms.
Glycosylation site binding protein and protein disulfide isomerase are identical and essential for cell viability in yeast.
Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum.
Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities.
Molecular structure of a yeast gene, PDI1, encoding protein disulfide isomerase that is essential for cell growth.
Protein disulfide isomerase is essential for viability in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae TRG1 gene is essential for growth and encodes a lumenal endoplasmic reticulum glycoprotein involved in the maturation of vacuolar carboxypeptidase.
The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase.
The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites.
Initiation of glycogen synthesis in yeast. Requirement of multiple tyrosine residues for function of the self-glucosylating Glg proteins in vivo.
Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae.
Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results in a reduced bacterial glycogen accumulation.
A faux 3'-UTR promotes aberrant termination and triggers nonsense-mediated mRNA decay.
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
Analysis of the proteome of Saccharomyces cerevisiae for methylarginine.
Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae.
Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G.
Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae.
Circularization of mRNA by eukaryotic translation initiation factors.
Differential effects of aromatic and charged residue substitutions in the RNA binding domains of the yeast poly(A)-binding protein.
Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast.
PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae.
Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation.
Poly(A)-binding protein acts in translation termination via eukaryotic release factor 3 interaction and does not influence [PSI(+)] propagation.
Positive and negative regulation of poly(A) nuclease.
Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins.
RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G.
Solution structure of the orphan PABC domain from Saccharomyces cerevisiae poly(A)-binding protein.
Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs.
The GTP-binding release factor eRF3 as a key mediator coupling translation termination to mRNA decay.
The eukaryotic mRNA decapping protein Dcp1 interacts physically and functionally with the eIF4F translation initiation complex.
The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation.
The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities.
The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation.
The poly(A)-poly(A)-binding protein complex is a major determinant of mRNA stability in vitro.
The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes.
The yeast poly(A)-binding protein Pab1p stimulates in vitro poly(A)-dependent and cap-dependent translation by distinct mechanisms.
The yeast protein encoded by PUB1 binds T-rich single stranded DNA.
Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation.
Translation termination factor eRF3 mediates mRNA decay through the regulation of deadenylation.
Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro.
Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export.
Yeast poly(A)-binding protein, Pab1, and PAN, a poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis to export.
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation.
The RAVE complex is essential for stable assembly of the yeast V-ATPase.
A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function.
A functional chaperone triad on the yeast ribosome.
Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.
Divergent functional properties of the ribosome-associated molecular chaperone Ssb compared with other Hsp70s.
Gene linkage of two-dimensional polyacrylamide gel electrophoresis resolved proteins from isogene families in Saccharomyces cerevisiae by microsequencing of in-gel trypsin generated peptides.
Hsp110 cooperates with different cytosolic HSP70 systems in a pathway for de novo folding.
Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction.
SSB, encoding a ribosome-associated chaperone, is coordinately regulated with ribosomal protein genes.
Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila.
The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis.
The molecular chaperone Ssb from Saccharomyces cerevisiae is a component of the ribosome-nascent chain complex.
The translation machinery and 70 kd heat shock protein cooperate in protein synthesis.
The yeast Hsp110 Sse1 functionally interacts with the Hsp70 chaperones Ssa and Ssb.
Yeast Hsp70 RNA levels vary in response to the physiological status of the cell.
A highly redundant gene network controls assembly of the outer spore wall in S. cerevisiae.
Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae.
Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of psi(2819) in 21S mitochondrial ribosomal RNA.
Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA.
A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation.
Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae.
Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae.
The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth.
A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factor.
Nucleotide exchange on ARF mediated by yeast Gea1 protein.
The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon.
The small GTPase Arf1 modulates mitochondrial morphology and function.
Competitive organelle-specific adaptors recruit Vps13 to membrane contact sites.
SPO71 encodes a developmental stage-specific partner for Vps13 in Saccharomyces cerevisiae.
SPO71 mediates prospore membrane size and maturation in Saccharomyces cerevisiae.
The transcriptional program of sporulation in budding yeast.
Identification and metabolic role of the mitochondrial aspartate-glutamate transporter in Saccharomyces cerevisiae.
A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.
Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7 and a tRNA for arginine.
Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2.
Telomere length as a quantitative trait: genome-wide survey and genetic mapping of telomere length-control genes in yeast.
Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.
Molecular organization of the Ndc80 complex, an essential kinetochore component.
Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication.
The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation.
The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control.
The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity.
Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells.
Discrimination between paralogs using microarray analysis: application to the Yap1p and Yap2p transcriptional networks.
Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa.
Overexpression of YAP2, coding for a new yAP protein, and YAP1 in Saccharomyces cerevisiae alleviates growth inhibition caused by 1,10-phenanthroline.
Peroxiredoxin Ahp1 acts as a receptor for alkylhydroperoxides to induce disulfide bond formation in the Cad1 transcription factor.
Post-termination ribosome interactions with the 5'UTR modulate yeast mRNA stability.
Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance.
Regulation of yAP-1 nuclear localization in response to oxidative stress.
Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae.
The S. cerevisiae Yap1 and Yap2 transcription factors share a common cadmium-sensing domain.
The role of the YAP1 and YAP2 genes in the regulation of the adaptive oxidative stress responses of Saccharomyces cerevisiae.
Yeast bZip proteins mediate pleiotropic drug and metal resistance.
Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors.
An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase.
Analysis of yeast protein kinases using protein chips.
TORC1 promotes phosphorylation of ribosomal protein S6 via the AGC kinase Ypk3 in Saccharomyces cerevisiae.
The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates.
Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.
Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV.
Involvement of actin-related proteins in ATP-dependent chromatin remodeling.
A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1.
An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast.
Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.
Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase.
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1.
AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation.
PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol.
Pga1 is an essential component of glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae.
Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly.
A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation.
Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation.
Expanded lysine acetylation specificity of Gcn5 in native complexes.
Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae.
Molecular architecture of the S. cerevisiae SAGA complex.
Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family.
Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry.
SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription.
The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway.
Yeast TAFIIS in a multisubunit complex required for activated transcription.
Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism.
A chromatin remodelling complex involved in transcription and DNA processing.
A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins.
Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth.
Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone.
Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes.
Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex.
The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription.
Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome.
RRN3 gene of Saccharomyces cerevisiae encodes an essential RNA polymerase I transcription factor which interacts with the polymerase independently of DNA template.
SGD1 encodes an essential nuclear protein of Saccharomyces cerevisiae that affects expression of the GPD1 gene for glycerol 3-phosphate dehydrogenase.
Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II.
Yeast Isw1p forms two separable complexes in vivo.
A Saccharomyces cerevisiae protein-serine kinase related to mammalian glycogen synthase kinase-3 and the Drosophila melanogaster gene shaggy product.
Analysis of RIM11, a yeast protein kinase that phosphorylates the meiotic activator IME1.
Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes.
Essential functions of protein tyrosine phosphatases PTP2 and PTP3 and RIM11 tyrosine phosphorylation in Saccharomyces cerevisiae meiosis and sporulation.
MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase 3.
Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity.
Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex.
Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast.
Adaptor complex-independent clathrin function in yeast.
The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast.
A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.
A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties.
Plasma-membrane hyperpolarization diminishes the cation efflux via Nha1 antiporter and Ena ATPase under potassium-limiting conditions.
Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X.
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
Characterization of the Saccharomyces cerevisiae cdc42-1ts allele and new temperature-conditional-lethal cdc42 alleles.
Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae.
Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae.
Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle.
Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae.
De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks.
Identification of a cytosolically directed NADH dehydrogenase in mitochondria of Saccharomyces cerevisiae.
In vivo analysis of the mechanisms for oxidation of cytosolic NADH by Saccharomyces cerevisiae mitochondria.
Kinetic regulation of the mitochondrial glycerol-3-phosphate dehydrogenase by the external NADH dehydrogenase in Saccharomyces cerevisiae.
Mitochondrial respiratory electron carriers are involved in oxidative stress during heat stress in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH.
Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii.
Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae.
Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae.
Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.
The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.
The conformation of a-factor is not influenced by the S-prenylation of Cys12.
Molecular architecture of the TRAPPII complex and implications for vesicle tethering.
TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport.
TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion.
The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae.
Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy.
Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase.
AUR1, a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae: a study of defective morphologies in Aur1p-depleted cells.
Inhibition of inositol phosphorylceramide synthase by the cyclic peptide aureobasidin A.
Inositol phosphorylceramide synthase is located in the Golgi apparatus of Saccharomyces cerevisiae.
Kei1: a novel subunit of inositolphosphorylceramide synthase, essential for its enzyme activity and Golgi localization.
Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene.
The AUR1 gene in Saccharomyces cerevisiae encodes dominant resistance to the antifungal agent aureobasidin A (LY295337).
A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex.
Characterization of IKI1 and IKI3 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae.
Dimerization of elongator protein 1 is essential for Elongator complex assembly.
Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.
Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity.
Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex.
The Elp2 subunit is essential for elongator complex assembly and functional regulation.
A novel regulator of G protein signalling in yeast, Rgs2, downregulates glucose-activation of the cAMP pathway through direct inhibition of Gpa2.
Galpha subunit Gpa2 recruits kelch repeat subunits that inhibit receptor-G protein coupling during cAMP-induced dimorphic transitions in Saccharomyces cerevisiae.
Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions.
The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits.
A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast.
A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.
Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion.
Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles.
Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain.
Expression, purification, and assay of Sec12p: a Sar1p-specific GDP dissociation stimulator.
Genes that allow yeast cells to grow in the absence of the HDEL receptor.
Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
Identification of a gene required for membrane protein retention in the early secretory pathway.
Inhibition of GTP hydrolysis by Sar1p causes accumulation of vesicles that are a functional intermediate of the ER-to-Golgi transport in yeast.
Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies.
Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p.
Purification and functional properties of yeast Sec12 GEF.
Reconstitution of GTP-binding Sar1 protein function in ER to Golgi transport.
Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins.
SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER.
Sec12p requires Rer1p for sorting to coatomer (COPI)-coated vesicles and retrieval to the ER.
Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER.
Selective packaging of cargo molecules into endoplasmic reticulum-derived COPII vesicles.
Structural and functional dissection of a membrane glycoprotein required for vesicle budding from the endoplasmic reticulum.
The early secretory pathway contributes to autophagy in yeast.
The yeast Sar exchange factor Sec12, and its higher organism orthologs, fold as beta-propellers.
Genomic exploration of the hemiascomycetous yeasts: 4. The genome of Saccharomyces cerevisiae revisited.
GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid.
Phosphatidylethanolamine is the donor of the phosphorylethanolamine linked to the alpha1,4-linked mannose of yeast GPI structures.
Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis.
The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames.
The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects.
Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant.
Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase.
Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.
Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae.
Vps13 is required for the packaging of the ER into autophagosomes during ER-phagy.
Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p.
Crystal structure of an alpha/beta serine hydrolase (YDR428C) from Saccharomyces cerevisiae at 1.85 A resolution.
Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence.
Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast.
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake.
Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange.
Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae.
ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance.
Allelism of SNQ1 and ATR1, genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide and aminotriazole.
CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.
Specificity of Processing alpha-glucosidase I is guided by the substrate conformation: crystallographic and in silico studies.
The yeast CWH41 gene encodes glucosidase I.
Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase.
Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs.
Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes.
Structure and transcriptional control of the Saccharomyces cerevisiae POX1 gene encoding acyl-coenzyme A oxidase.
Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae.
Overexpression of a sterol C-24(28) reductase increases ergosterol production in Saccharomyces cerevisiae.
The YGL022 gene encodes a putative transport protein.
A conserved family of proteins facilitates nascent lipid droplet budding from the ER.
Architecture of lipid droplets in endoplasmic reticulum is determined by phospholipid intrinsic curvature.
Cloning and sequence of the SCS3 gene which is required for inositol prototrophy in Saccharomyces cerevisiae.
FIT2 is an acyl-coenzyme A diphosphatase crucial for endoplasmic reticulum homeostasis.
Fat storage-inducing transmembrane (FIT or FITM) proteins are related to lipid phosphatase/phosphotransferase enzymes.
Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8 kb DNA fragment of yeast chromosome VII.
Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.
Dsl1p, an essential component of the Golgi-endoplasmic reticulum retrieval system in yeast, uses the same sequence motif to interact with different subunits of the COPI vesicle coat.
Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation.
Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2.
GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.
Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation.
Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells.
Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification.
The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits.
A primordial tRNA modification required for the evolution of life?
Retrograde nuclear import of tRNA precursors is required for modified base biogenesis in yeast.
The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.
Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase.
Biochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase.
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.
Nup192p is a conserved nucleoporin with a preferential location at the inner site of the nuclear membrane.
Structure, dynamics, evolution, and function of a major scaffold component in the nuclear pore complex.
Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction.
A DNA damage response pathway controlled by Tel1 and the Mre11 complex.
A mutation in yeast Tel1p that causes differential effects on the DNA damage checkpoint and telomere maintenance.
ATM-related Tel1 associates with double-strand breaks through an Xrs2-dependent mechanism.
Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins.
Distribution and dynamics of chromatin modification induced by a defined DNA double-strand break.
Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression.
Lcd1p recruits Mec1p to DNA lesions in vitro and in vivo.
Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase.
Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways.
Slx4 becomes phosphorylated after DNA damage in a Mec1/Tel1-dependent manner and is required for repair of DNA alkylation damage.
Suppression of spontaneous chromosomal rearrangements by S phase checkpoint functions in Saccharomyces cerevisiae.
TEL1, a gene involved in controlling telomere length in S. cerevisiae, is homologous to the human ataxia telangiectasia gene.
The yeast Xrs2 complex functions in S phase checkpoint regulation.
Yeast histone 2A serine 129 is essential for the efficient repair of checkpoint-blind DNA damage.
Contribution of the endoplasmic reticulum to peroxisome formation.
Farnesylation of Pex19p is not essential for peroxisome biogenesis in yeast and mammalian cells.
Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals.
Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome.
Pex19p binds Pex30p and Pex32p at regions required for their peroxisomal localization but separate from their peroxisomal targeting signals.
Pex19p, a farnesylated protein essential for peroxisome biogenesis.
Pex19p-dependent targeting of Pex17p, a peripheral component of the peroxisomal protein import machinery.
Pex3p initiates the formation of a preperoxisomal compartment from a subdomain of the endoplasmic reticulum in Saccharomyces cerevisiae.
Saccharomyces cerevisiae Pex3p and Pex19p are required for proper localization and stability of peroxisomal membrane proteins.
The dynamin-like protein Vps1p of the yeast Saccharomyces cerevisiae associates with peroxisomes in a Pex19p-dependent manner.
A late form of nucleophagy in Saccharomyces cerevisiae.
A protein conjugation system essential for autophagy.
A unified nomenclature for yeast autophagy-related genes.
An interrelationship between autophagy and filamentous growth in budding yeast.
Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway.
Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways.
Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site.
Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy.
Crystallization of the Atg12-Atg5 conjugate bound to Atg16 by the free-interface diffusion method.
Expression, purification and crystallization of the Atg5-Atg16 complex essential for autophagy.
Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.
Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.
Hierarchy of Atg proteins in pre-autophagosomal structure organization.
Impairing the bioenergetic status and the biogenesis of mitochondria triggers mitophagy in yeast.
In vivo reconstitution of autophagy in Saccharomyces cerevisiae.
Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.
Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions.
Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation.
Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex.
Piecemeal microautophagy of the nucleus requires the core macroautophagy genes.
Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy.
Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae.
Structural and functional analyses of APG5, a gene involved in autophagy in yeast.
Structure of Atg5.Atg16, a complex essential for autophagy.
Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation.
Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy.
The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.
The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation.
The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.
The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators.
Uth1p is involved in the autophagic degradation of mitochondria.
The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII.
A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
Dual role of mitofilin in mitochondrial membrane organization and protein biogenesis.
MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization.
The mitochondrial contact site complex, a determinant of mitochondrial architecture.
Uniform nomenclature for the mitochondrial contact site and cristae organizing system.
Cloning and characterization of COX14, whose product is required for assembly of yeast cytochrome oxidase.
Characterization of the Saccharomyces cerevisiae homolog of the melatonin rhythm enzyme arylalkylamine N-acetyltransferase (EC 2.3.1.87).
A stress-responsive system for mitochondrial protein degradation.
Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins.
HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins.
Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone.
Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene.
Role of the ubiquitin-selective CDC48(UFD1/NPL4) chaperone (segregase) in ERAD of OLE1 and other substrates.
Sequence and function analysis of a 4.3 kb fragment of Saccharomyces cerevisiae chromosome II including three open reading frames.
Substrate processing by the Cdc48 ATPase complex is initiated by ubiquitin unfolding.
The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol.
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation.
DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII.
Different import pathways through the mitochondrial intermembrane space for inner membrane proteins.
Import of small Tim proteins into the mitochondrial intermembrane space.
The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex.
The role of the TIM8-13 complex in the import of Tim23 into mitochondria.
The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins.
Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria.
Genetic evidence for a SPO1-dependent signaling pathway controlling meiotic progression in yeast.
Spo1, a phospholipase B homolog, is required for spindle pole body duplication during meiosis in Saccharomyces cerevisiae.
The SPO1 gene product required for meiosis in yeast has a high similarity to phospholipase B enzymes.
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein.
Human CPR (cell cycle progression restoration) genes impart a Far-phenotype on yeast cells.
The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes.
Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames.
Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs.
Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues.
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase.
TEP1, the yeast homolog of the human tumor suppressor gene PTEN/MMAC1/TEP1, is linked to the phosphatidylinositol pathway and plays a role in the developmental process of sporulation.
Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes.
Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells.
Glyceraldehyde-3-phosphate dehydrogenase catalyzed hydration of the 5-6 double bond of reduced beta-nicotinamide adenine dinucleotide (betaNADH). Formation of beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide adenine dinucleotide.
Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes.
Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
TDH2 is linked to MET3 on chromosome X of Saccharomyces cerevisiae.
The action of glyceraldehyde-3-phosphate dehydrogenase on reduced diphosphopyridine nucleotide.
A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3.
Molecular architecture, structure-function relationship, and importance of the Elp3 subunit for the RNA binding of holo-elongator.
Glycine and folate ameliorate models of congenital sideroblastic anemia.
YMC1, a yeast gene encoding a new putative mitochondrial carrier protein.
Cox18p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner membrane.
Peripheral mitochondrial inner membrane protein, Mss2p, required for export of the mitochondrially coded Cox2p C tail in Saccharomyces cerevisiae.
The nuclear-encoded MSS2 gene is involved in the expression of the mitochondrial cytochrome-c oxidase subunit 2 (Cox2).
The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames.
Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule a-agglutinin.
Intragenic tandem repeats generate functional variability.
Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin.
Posttranslational modifications required for cell surface localization and function of the fungal adhesin Aga1p.
The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a-agglutinin.
Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum.
The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation.
Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae.
YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex.
Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases.
Nucleotide sequence analysis of a 40 kb segment on the right arm of yeast chromosome XV reveals 18 open reading frames including a new pyruvate kinase and three homologues to chromosome I genes.
The MRE4 gene encodes a novel protein kinase homologue required for meiotic recombination in Saccharomyces cerevisiae.
A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast.
A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae.
An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.
Mutations in conserved yeast DNA primase domains impair DNA replication in vivo.
Shared active site architecture between the large subunit of eukaryotic primase and DNA photolyase.
The yeast DNA polymerase-primase complex: genes and proteins.
Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors.
Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase.
Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase.
Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase.
3-isopropylmalate is the major endogenous substrate of the Saccharomyces cerevisiae trans-aconitate methyltransferase.
Identification of the gene and characterization of the activity of the trans-aconitate methyltransferase from Saccharomyces cerevisiae.
Secreted 3-isopropylmalate methyl ester signals invasive growth during amino acid starvation in Saccharomyces cerevisiae.
A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae.
A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae.
Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae.
Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V.
Identification of an organelle-specific myosin V receptor.
Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.
Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.
Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p.
Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.
Myosin V orientates the mitotic spindle in yeast.
Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70.
Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.
Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables.
Structurally conserved interaction of Lgl family with SNAREs is critical to their cellular function.
The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.
The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function.
The terminal tail region of a yeast myosin-V mediates its attachment to vacuole membranes and sites of polarized growth.
The unconventional myosin, Myo2p, is a calmodulin target at sites of cell growth in Saccharomyces cerevisiae.
The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors.
The yeast kinesin-related protein Smy1p exerts its effects on the class V myosin Myo2p via a physical interaction.
Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications.
Two distinct regions in a yeast myosin-V tail domain are required for the movement of different cargoes.
Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.
A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery.
An ER-mitochondria tethering complex revealed by a synthetic biology screen.
Evolutionary conservation of biogenesis of beta-barrel membrane proteins.
Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p.
Machinery for protein sorting and assembly in the mitochondrial outer membrane.
Maintenance of mitochondrial morphology is linked to maintenance of the mitochondrial genome in Saccharomyces cerevisiae.
Mitochondrial protein sorting: differentiation of beta-barrel assembly by Tom7-mediated segregation of Mdm10.
Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene.
Regulation of mitochondrial morphology and inheritance by Mdm10p, a protein of the mitochondrial outer membrane.
Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes.
The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane.
The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria.
Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex.
Aberrant processing of the WSC family and Mid2p cell surface sensors results in cell death of Saccharomyces cerevisiae O-mannosylation mutants.
Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves.
Membrane association is a determinant for substrate recognition by PMT4 protein O-mannosyltransferases.
O-glycosylation as a sorting determinant for cell surface delivery in yeast.
O-glycosylation of Axl2/Bud10p by Pmt4p is required for its stability, localization, and function in daughter cells.
PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae.
Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5.
Protein-O-glycosylation in yeast: protein-specific mannosyltransferases.
The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital.
Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p.
Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing.
Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p.
Composition and functional characterization of the yeast spliceosomal penta-snRNP.
Elevated levels of a U4/U6.U5 snRNP-associated protein, Spp381p, rescue a mutant defective in spliceosome maturation.
A highly conserved binding site in vesicle-associated membrane protein-associated protein (VAP) for the FFAT motif of lipid-binding proteins.
Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae.
Gene recruitment of the activated INO1 locus to the nuclear membrane.
Structural basis of FFAT motif-mediated ER targeting.
The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism.
The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry.
Cytoplasmic dynein is required for normal nuclear segregation in yeast.
Disruption of mitotic spindle orientation in a yeast dynein mutant.
The offloading model for dynein function: differential function of motor subunits.
Sequencing of a 9.2 kb telomeric fragment from the right arm of Saccharomyces cerevisiae chromosome XIV.
A new essential gene of Saccharomyces cerevisiae, a defect in it may result in instability of nucleus.
N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome.
Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae.
Characterization of the yeast KEX1 gene product: a carboxypeptidase involved in processing secreted precursor proteins.
Chromosomal and nonchromosomal mutations affecting the 'killer character' of Saccharomyces cerevisiae.
Crystal structure of Kex1deltap, a prohormone-processing carboxypeptidase from Saccharomyces cerevisiae.
Crystallization of a soluble form of the Kex1p serine carboxypeptidase from Saccharomyces cerevisiae.
Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin. Requirement of a carboxypeptidase B-like activity for maturation.
Endocytotic uptake and retrograde transport of a virally encoded killer toxin in yeast.
Expression, purification, and characterization of the yeast KEX1 gene product, a polypeptide precursor processing carboxypeptidase.
Hormone (pheromone) processing enzymes in yeast. The carboxy-terminal processing enzyme of the mating pheromone alpha-factor, carboxypeptidase ysc alpha, is absent in alpha-factor maturation-defective kex1 mutant cells.
Kex1 protease is involved in yeast cell death induced by defective N-glycosylation, acetic acid, and chronological aging.
Mutational analysis of K28 preprotoxin processing in the yeast Saccharomyces cerevisiae.
The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating.
Two chromosomal genes required for killing expression in killer strains of Saccharomyces cerevisiae.
Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and alpha-factor precursor processing.
Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane.
Reverse transcriptase and integrase of the Saccharomyces cerevisiae Ty1 element.
Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19.
Localization of proteins that are coordinately expressed with Cln2 during the cell cycle.
Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19.
Slk19p is a centromere protein that functions to stabilize mitotic spindles.
Slk19p is necessary to prevent separation of sister chromatids in meiosis I.
Targets of the cyclin-dependent kinase Cdk1.
The Saccharomyces cerevisiae centromere protein Slk19p is required for two successive divisions during meiosis.
The yeast kinetochore protein Slk19 is required to prevent aberrant chromosome segregation in meiosis and mitosis.
A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae.
Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.
The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p.
A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p, and identification of a Mid2p-interacting protein, Zeo1p, that modulates the PKC1-MPK1 cell integrity pathway.
Mid2 is a putative sensor for cell integrity signaling in Saccharomyces cerevisiae.
Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast.
Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway.
The MID2 gene encodes a putative integral membrane protein with a Ca(2+)-binding domain and shows mating pheromone-stimulated expression in Saccharomyces cerevisiae.
The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae.
The SMS1 gene encoding a serine-rich transmembrane protein suppresses the temperature sensitivity of the htr1 disruptant in Saccharomyces cerevisiae.
Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1.
Isolation and characterization of QCR10, the nuclear gene encoding the 8.5-kDa subunit 10 of the Saccharomyces cerevisiae cytochrome bc1 complex.
Point mutation in cytochrome b of yeast ubihydroquinone:cytochrome-c oxidoreductase causing myxothiazol resistance and facilitated dissociation of the iron-sulfur subunit.
A role for N-glycanase in the cytosolic turnover of glycoproteins.
Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p).
Misfolding of glycoproteins is a prerequisite for peptide: N-glycanase mediated deglycosylation.
PNG1, a yeast gene encoding a highly conserved peptide:N-glycanase.
Rad23 provides a link between the Png1 deglycosylating enzyme and the 26 S proteasome in yeast.
Site-directed mutagenesis study of yeast peptide:N-glycanase. Insight into the reaction mechanism of deglycosylation.
Site-specific labeling of cytoplasmic peptide: N-glycanase by N,N'-diacetylchitobiose-related compounds.
Structure of a peptide:N-glycanase-Rad23 complex: insight into the deglycosylation for denatured glycoproteins.
The N-terminus of yeast peptide: N-glycanase interacts with the DNA repair protein Rad23.
The Png1-Rad23 complex regulates glycoprotein turnover.
Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover.
Yeast N-glycanase distinguishes between native and non-native glycoproteins.
z-VAD-fmk inhibits peptide:N-glycanase and may result in ER stress.
DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae.
Evolutionarily conserved function of RRP36 in early cleavages of the pre-rRNA and production of the 40S ribosomal subunit.
The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes.
Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.
Cycloheximide resistance in yeast: the gene and its protein.
Transcriptional elements of the yeast ribosomal protein gene CYH2.
A proteolytic pathway that recognizes ubiquitin as a degradation signal.
The Uba2 and Ufd1 proteins of Saccharomyces cerevisiae interact with poly(A) polymerase and affect the polyadenylation activity of cell extracts.
Ufd1 exhibits the AAA-ATPase fold with two distinct ubiquitin interaction sites.
Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast.
Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene.
A new yeast poly(A) polymerase complex involved in RNA quality control.
Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase.
Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p.
RNA degradation by the exosome is promoted by a nuclear polyadenylation complex.
Structure and function of the polymerase core of TRAMP, a RNA surveillance complex.
A yeast model system for functional analysis of the Niemann-Pick type C protein 1 homolog, Ncr1p.
Mutagenesis of the putative sterol-sensing domain of yeast Niemann Pick C-related protein reveals a primordial role in subcellular sphingolipid distribution.
Ncr1p, the yeast ortholog of mammalian Niemann Pick C1 protein, is dispensable for endocytic transport.
Structure of a yeast hypothetical protein selected by a structural genomics approach.
The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein.
A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNA synthetases.
Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes.
Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs.
Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1.
Cytoplasmic methionyl-tRNA synthetase from Bakers' yeast. A monomer with a post-translationally modified N-terminus.
Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.
Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase.
Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes.
The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p.
The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases.
DNA sequence analysis of a 13 kbp fragment of the left arm of yeast chromosome XV containing seven new open reading frames.
New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis.
Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).
Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase.
The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner.
DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases.
Identification of nuclear genes which participate in mitochondrial translation in Saccharomyces cerevisiae.
MBR1 and MBR3, two related yeast genes that can suppress the growth defect of hap2, hap3 and hap4 mutants.
Sequence analysis of a 3.5 Kb EcoRI fragment from the left arm of Saccharomyces cerevisiae chromosome XI reveals the location of the MBR1 gene and a sequence related to a GTPase-activating protein.
The MBR1 gene from Saccharomyces cerevisiae is activated by and required for growth under sub-optimal conditions.
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
Functional domains of a positive regulatory protein, PHO4, for transcriptional control of the phosphatase regulon in Saccharomyces cerevisiae.
Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4.
Mode of expression of the positive regulatory genes PHO2 and PHO4 of the phosphatase regulon in Saccharomyces cerevisiae.
Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85.
Regulation of PHO4 nuclear localization by the PHO80-PHO85 cyclin-CDK complex.
Roles of phosphorylation sites in regulating activity of the transcription factor Pho4.
The activation domain of a basic helix-loop-helix protein is masked by repressor interaction with domains distinct from that required for transcription regulation.
The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal.
The yeast regulatory gene PHO4 encodes a helix-loop-helix motif.
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.
Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body.
Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif.
The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein.
Two new S-phase-specific genes from Saccharomyces cerevisiae.
An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes.
The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus.
Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion.
Vps51p links the VFT complex to the SNARE Tlg1p.
Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p.
Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi.
Characterization of chitin synthase 2 of Saccharomyces cerevisiae. Implication of two highly conserved domains as possible catalytic sites.
Similar and different domains of chitin synthases 1 and 2 of S. cerevisiae: two isozymes with distinct functions.
A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae.
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins.
Crystal structure of the 14-subunit RNA polymerase I.
Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I.
Functional architecture of RNA polymerase I.
Localization of the yeast RNA polymerase I-specific subunits.
RNA polymerase I structure and transcription regulation.
Role of second-largest RNA polymerase I subunit Zn-binding domain in enzyme assembly.
Rpa12p, a conserved RNA polymerase I subunit with two functional domains.
Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I.
The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.
Predicting protein functions from redundancies in large-scale protein interaction networks.
The 1.8-A resolution crystal structure of YDR533Cp from Saccharomyces cerevisiae: a member of the DJ-1/ThiJ/PfpI superfamily.
Yeast DJ-1 superfamily members are required for diauxic-shift reprogramming and cell survival in stationary phase.
Enhancement of superficial pseudohyphal growth by overexpression of the SFG1 gene in yeast Saccharomyces cerevisiae.
Organization of mitochondrial gene expression in two distinct ribosome-containing assemblies.
YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae.
Accumulation of phosphorylated sphingoid long chain bases results in cell growth inhibition in Saccharomyces cerevisiae.
Calcium influx and signaling in yeast stimulated by intracellular sphingosine 1-phosphate accumulation.
Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae.
Role for de novo sphingoid base biosynthesis in the heat-induced transient cell cycle arrest of Saccharomyces cerevisiae.
The LCB4 (YOR171c) and LCB5 (YLR260w) genes of Saccharomyces encode sphingoid long chain base kinases.
Mitochondrial splicing requires a protein from a novel helicase family.
Overexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase.
Overexpression of DEAD box protein pMSS116 promotes ATP-dependent splicing of a yeast group II intron in vitro.
The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function.
Nuclear and mitochondrial inheritance in yeast depends on novel cytoplasmic structures defined by the MDM1 protein.
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits.
Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.
The core meiotic transcriptome in budding yeasts.
A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I.
NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA.
Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae.
The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control.
The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis.
Disruption of the yeast nuclear PET54 gene blocks excision of mitochondrial intron aI5 beta from pre-mRNA for cytochrome c oxidase subunit I.
MSS18, a yeast nuclear gene involved in the splicing of intron aI5 beta of the mitochondrial cox1 transcript.
The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae.
The nuclear SUV3-1 mutation affects a variety of post-transcriptional processes in yeast mitochondria.
Two group I mitochondrial introns in the cob-box and coxI genes require the same MRS1/PET157 nuclear gene product for splicing.
The VRG4 gene is required for GDP-mannose transport into the lumen of the Golgi in the yeast, Saccharomyces cerevisiae.
An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes.
Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways.
Exploring the topology of the Gid complex, the E3 ubiquitin ligase involved in catabolite-induced degradation of gluconeogenic enzymes.
Gid9, a second RING finger protein contributes to the ubiquitin ligase activity of the Gid complex required for catabolite degradation.
Proteins of newly isolated mutants and the amino-terminal proline are essential for ubiquitin-proteasome-catalyzed catabolite degradation of fructose-1,6-bisphosphatase of Saccharomyces cerevisiae.
The yeast GID complex, a novel ubiquitin ligase (E3) involved in the regulation of carbohydrate metabolism.
Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome.
Elongation factor methyltransferase 3 - A novel eukaryotic lysine methyltransferase.
Stoichiometry of Saccharomyces cerevisiae lysine methylation: insights into non-histone protein lysine methyltransferase activity.
Translational roles of elongation factor 2 protein lysine methylation.
A comparison of yeast mannan mutants by electron microscopy.
Biosynthesis of yeast mannan. Properties of a mannosylphosphate transferase in Saccharomyces cerevisiae.
Biosynthesis of yeast mannoproteins. Synthesis of mannan outer chain and of dolichol derivatives.
Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation.
Genetic control of yeast mannan structure. Complementation studies and properties of mannan mutants.
Genetic control of yeast mannan structure. Isolation and characterization of mannan mutants.
Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae.
Golgi localization of glycosyltransferases requires a Vps74p oligomer.
Identification and characterization of a gene and protein required for glycosylation in the yeast Golgi.
Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan.
The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein.
Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important for growth, functionally overlapping with the event regulated by calcineurin- and Mpk1-mediated pathways.
Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs.
Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae.
A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae.
Identification of genes required for protection from doxorubicin by a genome-wide screen in Saccharomyces cerevisiae.
3D solution structure of copper and silver-substituted yeast metallothioneins.
Copper metallothionein of yeast, structure of the gene, and regulation of expression.
High resolution solution structure of the protein part of Cu7 metallothionein.
Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast.
Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast.
The crystal structure of yeast copper thionein: the solution of a long-lasting enigma.
X-ray absorption studies of yeast copper metallothionein.
Yeast metallothionein. Sequence and metal-binding properties.
Conservation of mitotic controls in fission and budding yeasts.
The SIT4 protein phosphatase functions in late G1 for progression into S phase.
The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function.
A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria.
DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames.
Saccharomyces cerevisiae expresses three functionally distinct homologues of the nramp family of metal transporters.
The family of SMF metal ion transporters in yeast cells.
Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein.
Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites.
Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae.
A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein.
Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk.
Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae.
Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the process.
The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins.
Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins.
cDNA and deduced amino acid sequence of acidic ribosomal protein A2 from Saccharomyces cerevisiae.
A new antioxidant with alkyl hydroperoxide defense properties in yeast.
Attachment of the ubiquitin-related protein Urm1p to the antioxidant protein Ahp1p.
Characterization of the yeast peroxiredoxin Ahp1 in its reduced active and overoxidized inactive forms using NMR.
Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae.
In vivo characterization of a thioredoxin H target protein defines a new peroxiredoxin family.
Involvement of thioredoxin peroxidase type II (Ahp1p) of Saccharomyces cerevisiae in Mn2+ homeostasis.
Purification and characterization of a second type thioredoxin peroxidase (type II TPx) from Saccharomyces cerevisiae.
Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier.
Structural snapshots of yeast alkyl hydroperoxide reductase Ahp1 peroxiredoxin reveal a novel two-cysteine mechanism of electron transfer to eliminate reactive oxygen species.
A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining.
Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III.
Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway.
The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes.
The yeast Saccharomyces cerevisiae DNA polymerase IV: possible involvement in double strand break DNA repair.
Yeast open reading frame YCR14C encodes a DNA beta-polymerase-like enzyme.
A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae.
Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication.
Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4.
Competing crossover pathways act during meiosis in Saccharomyces cerevisiae.
Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization.
Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease.
Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis.
Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage.
Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae.
Substrate specificity of the Saccharomyces cerevisiae Mus81-Mms4 endonuclease.
The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions.
The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast.
The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10.
Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor.
A widely distributed 'CAT' family of repetitive DNA sequences.
D-arabinose dehydrogenase and its gene from Saccharomyces cerevisiae.
A family of hexosephosphate mutases in Saccharomyces cerevisiae.
Genetic control of phosphoglucomutase variants in Saccharomyces cerevisiae.
Phosphoglucomutase. V. Multiple forms of phosphoglucomutase.
The effect of mutation of two forms of phosphoglucomutase in Saccharomyces.
Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum.
The yeast Apq12 protein affects nucleocytoplasmic mRNA transport.
The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes.
PET112, a Saccharomyces cerevisiae nuclear gene required to maintain rho+ mitochondrial DNA.
Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS.
Biochemical and structural studies of conserved Maf proteins revealed nucleotide pyrophosphatases with a preference for modified nucleotides.
Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames.
Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo.
Dissection of the molecular basis of mycophenolate resistance in Saccharomyces cerevisiae.
Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast.
Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevisiae.
Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids.
HSP12 is essential for biofilm formation by a Sardinian wine strain of Saccharomyces cerevisiae.
HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function.
Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations.
A novel family of cell wall-related proteins regulated differently during the yeast life cycle.
Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages.
Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall.
Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway.
Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae.
Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae.
Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae.
Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.
A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities.
Elimination of replication block protein Fob1 extends the life span of yeast mother cells.
Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I.
Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae.
The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork.
Effects of oxygen concentration on the expression of cytochrome c and cytochrome c oxidase genes in yeast.
Inverse regulation of the yeast COX5 genes by oxygen and heme.
Splicing of a yeast intron containing an unusual 5' junction sequence.
Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V.
Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase.
A large-scale full-length cDNA analysis to explore the budding yeast transcriptome.
The SKS1 protein kinase is a multicopy suppressor of the snf3 mutation of Saccharomyces cerevisiae.
Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes.
Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.
The Saccharomyces cerevisiae aldose reductase is implied in the metabolism of methylglyoxal in response to stress conditions.
The structure and function of yeast xylose (aldose) reductases.
Three genes whose expression is induced by stress in Saccharomyces cerevisiae.
Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.
Disruption of mechanisms that prevent rereplication triggers a DNA damage response.
Esc4/Rtt107 and the control of recombination during replication.
Genetic analysis of Saccharomyces cerevisiae H2A serine 129 mutant suggests a functional relationship between H2A and the sister-chromatid cohesion partners Csm3-Tof1 for the repair of topoisomerase I-induced DNA damage.
Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae.
Identification of protein complexes required for efficient sister chromatid cohesion.
Mrc1 and Tof1 promote replication fork progression and recovery independently of Rad53.
S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex.
The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae.
Tof1p regulates DNA damage responses during S phase in Saccharomyces cerevisiae.
Uncoupling of unwinding from DNA synthesis implies regulation of MCM helicase by Tof1/Mrc1/Csm3 checkpoint complex.
Association of yeast SAP1, a novel member of the 'AAA' ATPase family of proteins, with the chromatin protein SIN1.
A 26 kDa yeast DNA topoisomerase I fragment: crystallographic structure and mechanistic implications.
Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene.
Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I.
Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I.
New protein functions in yeast chromosome VIII.
Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast.
Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.
Prediction of novel Bag-1 homologs based on structure/function analysis identifies Snl1p as an Hsp70 co-chaperone in Saccharomyces cerevisiae.
The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function.
Cloning and expression of two chitin deacetylase genes of Saccharomyces cerevisiae.
Expression, purification, and characterization of a cobalt-activated chitin deacetylase (Cda2p) from Saccharomyces cerevisiae.
Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases.
Ca2+/calmodulin-dependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells.
Elm1p is one of three upstream kinases for the Saccharomyces cerevisiae SNF1 complex.
Mammalian TAK1 activates Snf1 protein kinase in yeast and phosphorylates AMP-activated protein kinase in vitro.
Pak1 protein kinase regulates activation and nuclear localization of Snf1-Gal83 protein kinase.
Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae.
Regulation of snf1 protein kinase in response to environmental stress.
Regulatory domains of Snf1-activating kinases determine pathway specificity.
Role of Tos3, a Snf1 protein kinase kinase, during growth of Saccharomyces cerevisiae on nonfermentable carbon sources.
Snf1 kinase complexes with different beta subunits display stress-dependent preferences for the three Snf1-activating kinases.
The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene.
Yeast Pak1 kinase associates with and activates Snf1.
A new type of protein lysine methyltransferase trimethylates Lys-79 of elongation factor 1A.
A novel post-translational modification of yeast elongation factor 1A. Methylesterification at the C-terminus.
An aminoacylation-dependent nuclear tRNA export pathway in yeast.
Association of elongation factor 1 alpha and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein CAP.
Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery.
Characterization of yeast EF-1 alpha: non-conservation of post-translational modifications.
Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha.
Competition and cooperation amongst yeast elongation factors.
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex.
Elongation factor 1 alpha from Saccharomyces cerevisiae. Rapid large-scale purification and molecular characterization.
Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity.
Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.
Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae.
Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells.
Mutations in a GTP-binding motif of eukaryotic elongation factor 1A reduce both translational fidelity and the requirement for nucleotide exchange.
Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae.
Novel N-terminal and lysine methyltransferases that target translation elongation factor 1A in yeast and human.
Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast.
Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses.
Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.
Proteasome-mediated degradation of cotranslationally damaged proteins involves translation elongation factor 1A.
Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae.
Saccharomyces cerevisiae eukaryotic elongation factor 1A (eEF1A) is methylated at Lys-390 by a METTL21-like methyltransferase.
Site-directed mutagenesis of yeast eEF1A. Viable mutants with altered nucleotide specificity.
Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A:eEF1Balpha.
Structure of the two genes coding for polypeptide chain elongation factor 1 alpha (EF-1 alpha) from Saccharomyces cerevisiae.
TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene.
Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae.
Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI > 7) proteins.
Zinc-finger protein Zpr1 is a bespoke chaperone essential for eEF1A biogenesis.
A proteasome cap subunit required for spindle pole body duplication in yeast.
Isolation and characterization of SUG2. A novel ATPase family component of the yeast 26 S proteasome.
Functional analyses of PAU genes in Saccharomyces cerevisiae.
COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae.
Conservation of the COP9/signalosome in budding yeast.
Functional organization of the yeast proteome by systematic analysis of protein complexes.
The COP9 signalosome-like complex in S. cerevisiae and links to other PCI complexes.
Nucleotide sequence and characterization of the yeast PSS gene encoding phosphatidylserine synthase.
Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase.
The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme.
Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes.
Homologous subunits of 1,3-beta-glucan synthase are important for spore wall assembly in Saccharomyces cerevisiae.
A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae.
Analysis of nucleosome repositioning by yeast ISWI and Chd1 chromatin remodeling complexes.
Chd1 and yFACT act in opposition in regulating transcription.
Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes.
Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms.
Molecular implications of evolutionary differences in CHD double chromodomains.
RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA.
RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach.
Replication-independent assembly of nucleosome arrays in a novel yeast chromatin reconstitution system involves antisilencing factor Asf1p and chromodomain protein Chd1p.
Rtf1 is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification.
Structural polymorphism of chromodomains in Chd1.
The ISWI and CHD1 chromatin remodelling activities influence ADH2 expression and chromatin organization.
The chromo domain protein chd1p from budding yeast is an ATP-dependent chromatin-modifying factor.
Ecm11 is located in the cell nucleus throughout mitosis.
The Saccharomyces cerevisiae Pet309 protein is embedded in the mitochondrial inner membrane.
The product of the nuclear gene PET309 is required for translation of mature mRNA and stability or production of intron-containing RNAs derived from the mitochondrial COX1 locus of Saccharomyces cerevisiae.
CLN7 is an organellar chloride channel regulating lysosomal function.
The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames.
Analysis of a recombination hotspot for gene conversion occurring at the HIS2 gene of Saccharomyces cerevisiae.
Homodimeric quaternary structure is required for the in vivo function and thermal stability of Saccharomyces cerevisiae and Schizosaccharomyces pombe RNA triphosphatases.
Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability.
Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure.
Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. II. Catalytic properties.
Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. Large scale purification, subunit functions, and subcellular localization.
Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus.
Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1.
Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast.
Downregulation of autophagy by Met30-mediated Atg9 ubiquitination.
Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex.
Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p.
Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats.
The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle.
The abundance of Met30p limits SCF(Met30p) complex activity and is regulated by methionine availability.
The amino-terminal portion of the F-box protein Met30p mediates its nuclear import and assimilation into an SCF complex.
The yeast ubiquitin ligase SCFMet30 regulates heavy metal response.
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
Asf1 can promote trimethylation of H3 K36 by Set2.
Association of the histone methyltransferase Set2 with RNA polymerase II plays a role in transcription elongation.
Dimethylation of histone H3 at lysine 36 demarcates regulatory and nonregulatory chromatin genome-wide.
Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II.
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.
Set2-catalyzed methylation of histone H3 represses basal expression of GAL4 in Saccharomyces cerevisiae.
Structural analysis of WW domains and design of a WW prototype.
Structure and carboxyl-terminal domain (CTD) binding of the Set2 SRI domain that couples histone H3 Lys36 methylation to transcription.
The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II.
The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation.
The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation.
Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast.
Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast.
Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking.
Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs.
FEN2: a gene implicated in the catabolite repression-mediated regulation of ergosterol biosynthesis in yeast.
Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from strain AB972: evidence for a Ty insertion and functional analysis of open reading frame YCR28.
The fenpropimorph resistance gene FEN2 from Saccharomyces cerevisiae encodes a plasma membrane H+-pantothenate symporter.
Yeast chromosome III: new gene functions.
Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation.
The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames.
The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment.
Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames.
Molecular cloning and characterization of Saccharomyces cerevisiae RAD28, the yeast homolog of the human Cockayne syndrome A (CSA) gene.
Recovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair.
MINDY-1 is a member of an evolutionarily conserved and structurally distinct new family of deubiquitinating enzymes.
Identification and characterization of a second NMN adenylyltransferase gene in Saccharomyces cerevisiae.
Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels.
YCL047C/POF1 is a novel nicotinamide mononucleotide adenylyltransferase (NMNAT) in Saccharomyces cerevisiae.
A dominant negative mutation in Saccharomyces cerevisiae methionine aminopeptidase-1 affects catalysis and interferes with the function of methionine aminopeptidase-2.
Evidence that two zinc fingers in the methionine aminopeptidase from Saccharomyces cerevisiae are important for normal growth.
Molecular cloning, sequencing, deletion, and overexpression of a methionine aminopeptidase gene from Saccharomyces cerevisiae.
N-terminal methionine removal and methionine metabolism in Saccharomyces cerevisiae.
The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2.
The specificity in vivo of two distinct methionine aminopeptidases in Saccharomyces cerevisiae.
Yeast (Saccharomyces cerevisiae) methionine aminopeptidase I: rapid purification and improved activity assay.
Yeast methionine aminopeptidase I can utilize either Zn2+ or Co2+ as a cofactor: a case of mistaken identity?
Yeast methionine aminopeptidase type 1 is ribosome-associated and requires its N-terminal zinc finger domain for normal function in vivo.
Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase.
A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control.
The yeast gene MSC2, a member of the cation diffusion facilitator family, affects the cellular distribution of zinc.
Gene SNQ2 of Saccharomyces cerevisiae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases.
Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins.
DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae.
Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris.
Yeast KRE2 defines a new gene family encoding probable secretory proteins, and is required for the correct N-glycosylation of proteins.
Overexpression of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminum ion.
Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames.
Serine and threonine catabolism in Saccharomyces cerevisiae: the CHA1 polypeptide is homologous with other serine and threonine dehydratases.
Allosteric regulation provides a molecular mechanism for preferential utilization of the fully assembled dolichol-linked oligosaccharide by the yeast oligosaccharyltransferase.
An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene.
Rules for the recognition of dilysine retrieval motifs by coatomer.
Structure of the yeast oligosaccharyltransferase complex gives insight into eukaryotic N-glycosylation.
Studies of yeast oligosaccharyl transferase subunits using the split-ubiquitin system: topological features and in vivo interactions.
Subunits of the translocon interact with components of the oligosaccharyl transferase complex.
Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity.
The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast.
The N-oligosaccharyltransferase complex from yeast.
The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides.
The atomic structure of a eukaryotic oligosaccharyltransferase complex.
The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p.
The oligosaccharyltransferase complex from yeast.
The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro.
Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons.
Yeast oligosaccharyltransferase consists of two functionally distinct sub-complexes, specified by either the Ost3p or Ost6p subunit.
The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits.
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast.
Trm112 is required for Bud23-mediated methylation of the 18S rRNA at position G1575.
Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA.
Tandemly repeated 147 bp elements cause structural and functional variation in divergent MAL promoters of Saccharomyces cerevisiae.
Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis.
Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.
Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1.
Functional analysis of the yeast Ran exchange factor Prp20p: in vivo evidence for the RanGTP gradient model.
GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization.
Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae.
Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily.
A comprehensive two-hybrid analysis to explore the yeast protein interactome.
Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability.
The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae.
Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck.
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast.
The Saccharomyces cerevisiae DNA repair gene RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function.
Genome-wide identification of spliced introns using a tiling microarray.
Molecular analysis of the Saccharomyces cerevisiae YHR076w gene.
Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function.
The YHR076w gene encodes a type 2C protein phosphatase and represents the seventh PP2C gene in budding yeast.
The mitochondrial phosphatase PPTC7 orchestrates mitochondrial metabolism regulating coenzyme Q10 biosynthesis.
The phosphatase Ptc7 induces coenzyme Q biosynthesis by activating the hydroxylase Coq7 in yeast.
Genomic analysis of stationary-phase and exit in Saccharomyces cerevisiae: gene expression and identification of novel essential genes.
Molecular characterization of the SPT23 gene: a dosage-dependent suppressor of Ty-induced promoter mutations from Saccharomyces cerevisiae.
Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.
Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae.
Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae.
Yeast origin recognition complex functions in transcription silencing and DNA replication.
Yeast origin recognition complex is involved in DNA replication and transcriptional silencing.
Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.
Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae.
Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17.
Role of Tim21 in mitochondrial translocation contact sites.
An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase.
Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes.
Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for chromosome segregation in Saccharomyces cerevisiae.
Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex.
Biogenesis of the yeast vacuole (lysosome). Active site mutation in the vacuolar aspartate proteinase yscA blocks maturation of vacuolar proteinases.
Kinesin-related proteins required for assembly of the mitotic spindle.
PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.
The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.
The three-dimensional structure at 2.4-A resolution of glycosylated proteinase A from the lysosome-like vacuole of Saccharomyces cerevisiae.
Vacuolar and extracellular maturation of Saccharomyces cerevisiae proteinase A.
Synthetic lethality with conditional dbp6 alleles identifies Rsa1p, a nucleoplasmic protein involved in the assembly of 60S ribosomal subunits.
Genome-wide analysis of yeast transcription upon calcium shortage.
Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymes.
Systematic mutagenesis of the leucine-rich repeat (LRR) domain of CCR4 reveals specific sites for binding to CAF1 and a separate critical role for the LRR in CCR4 deadenylase activity.
Cloning and sequencing of the gene encoding the soluble fumarate reductase from Saccharomyces cerevisiae.
Investigation by 13C-NMR and tricarboxylic acid (TCA) deletion mutant analysis of pathways for succinate formation in Saccharomyces cerevisiae during anaerobic fermentation.
One of the fumarate reductase isoenzymes from Saccharomyces cerevisiae is encoded by the OSM1 gene.
Role in anaerobiosis of the isoenzymes for Saccharomyces cerevisiae fumarate reductase encoded by OSM1 and FRDS1.
Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth.
Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases.
Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake.
Biochemical basis of oxidative protein folding in the endoplasmic reticulum.
Ero1p oxidizes protein disulfide isomerase in a pathway for disulfide bond formation in the endoplasmic reticulum.
Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum.
Modulation of cellular disulfide-bond formation and the ER redox environment by feedback regulation of Ero1.
Oxidative protein folding in eukaryotes: mechanisms and consequences.
Structure of Ero1p, source of disulfide bonds for oxidative protein folding in the cell.
The C-terminal domain of yeast Ero1p mediates membrane localization and is essential for function.
The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum.
The FAD- and O(2)-dependent reaction cycle of Ero1-mediated oxidative protein folding in the endoplasmic reticulum.
Two pairs of conserved cysteines are required for the oxidative activity of Ero1p in protein disulfide bond formation in the endoplasmic reticulum.
Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p.
The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase.
Yeast adenylate cyclase catalytic domain is carboxy terminal.
Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast.
Der1p, a protein required for degradation of malfolded soluble proteins of the endoplasmic reticulum: topology and Der1-like proteins.
Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation.
N-terminal acetylation of the yeast Derlin Der1 is essential for Hrd1 ubiquitin-ligase activity toward luminal ER substrates.
Structural basis of ER-associated protein degradation mediated by the Hrd1 ubiquitin ligase complex.
Usa1 functions as a scaffold of the HRD-ubiquitin ligase.
Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex.
A phosphatase complex that dephosphorylates gamma-H2AX regulates DNA damage checkpoint recovery.
A role for Saccharomyces cerevisiae histone H2A in DNA repair.
An Nalpha-acetyltransferase responsible for acetylation of the N-terminal residues of histones H4 and H2A.
Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites.
DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain.
Esa1p is an essential histone acetyltransferase required for cell cycle progression.
Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification.
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications.
Lysine succinylation and lysine malonylation in histones.
Sequence around the centromere of Saccharomyces cerevisiae chromosome II: similarity of CEN2 to CEN4.
The two yeast histone H2A genes encode similar protein subtypes.
Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum.
Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate.
Quantitative high-content imaging identifies novel regulators of Neo1 trafficking at endosomes.
Retrieval of resident late-Golgi membrane proteins from the prevacuolar compartment of Saccharomyces cerevisiae is dependent on the function of Grd19p.
The phox homology (PX) domain protein interaction network in yeast.
Dual function of Sdh3 in the respiratory chain and TIM22 protein translocase of the mitochondrial inner membrane.
Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants.
A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin.
A yeast protein with homology to the beta-subunit of G proteins is involved in control of heme-regulated and catabolite-repressed genes.
Characterization of TUP1, a mediator of glucose repression in Saccharomyces cerevisiae.
Cloning of the yeast SFL2 gene: its disruption results in pleiotropic phenotypes characteristic for tup1 mutants.
Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo.
Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme.
Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress.
Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein.
Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.
Ssn6-Tup1 interacts with class I histone deacetylases required for repression.
Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast.
TUP1 utilizes histone H3/H2B-specific HDA1 deacetylase to repress gene activity in yeast.
The Cyc8 (Ssn6)-Tup1 corepressor complex is composed of one Cyc8 and four Tup1 subunits.
The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor.
The SFL2 (TUP1?) protein of Saccharomyces cerevisiae contains a repeating motif homologous to beta subunits of G proteins.
The WD repeats of Tup1 interact with the homeo domain protein alpha 2.
Tup1-Ssn6 interacts with multiple class I histone deacetylases in vivo.
Alteration of a novel dispensable mitochondrial ribosomal small-subunit protein, Rsm28p, allows translation of defective COX2 mRNAs.
High-resolution view of the yeast meiotic program revealed by ribosome profiling.
Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p.
Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Isolation of a Saccharomyces cerevisiae long chain fatty acyl:CoA synthetase gene (FAA1) and assessment of its role in protein N-myristoylation.
Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases.
The Sjogren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
The acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular utilization.
Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6.
Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily.
Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.
Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae.
The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.
The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein.
The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast.
A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae.
Structures of yeast glutathione-S-transferase Gtt2 reveal a new catalytic type of GST family.
DEAD-box RNA helicase Sub2 is required for expression of lacZ fusions in Saccharomyces cerevisiae and is a dosage-dependent suppressor of RLR1 (THO2).
Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase.
High-copy-number expression of Sub2p, a member of the RNA helicase superfamily, suppresses hpr1-mediated genomic instability.
Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56.
Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation.
Multiple roles for the yeast SUB2/yUAP56 gene in splicing.
Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.
Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p.
Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis.
TREX is a conserved complex coupling transcription with messenger RNA export.
The DECD box putative ATPase Sub2p is an early mRNA export factor.
The Saccharomyces cerevisiae Sub2 protein suppresses heterochromatic silencing at telomeres and subtelomeric genes.
The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability.
Isolation and characterization of the gene encoding yeast debranching enzyme.
Metal content and kinetic properties of yeast RNA lariat debranching enzyme Dbr1.
Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein.
RNA branching and debranching in the yeast retrovirus-like element Ty1.
Hyperosmotic stress response and regulation of cell wall integrity in Saccharomyces cerevisiae share common functional aspects.
Lre1 affects chitinase expression, trehalose accumulation and heat resistance through inhibition of the Cbk1 protein kinase in Saccharomyces cerevisiae.
Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae.
Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6.
Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases.
Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2.
The gene PPG encodes a novel yeast protein phosphatase involved in glycogen accumulation.
Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB.
DNA binding by yeast Mlh1 and Pms1: implications for DNA mismatch repair.
DNA binding properties of the yeast Msh2-Msh6 and Mlh1-Pms1 heterodimers.
Differential ATP binding and intrinsic ATP hydrolysis by amino-terminal domains of the yeast Mlh1 and Pms1 proteins.
Dissecting the architecture of a quantitative trait locus in yeast.
Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutations.
Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction.
Functional studies on the candidate ATPase domains of Saccharomyces cerevisiae MutLalpha.
Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair.
Novel PMS1 alleles preferentially affect the repair of primer strand loops during DNA replication.
A DBL-homologous region of the yeast CLS4/CDC24 gene product is important for Ca(2+)-modulated bud assembly.
Nucleotide sequence of the CLS4 (CDC24) gene of Saccharomyces cerevisiae.
Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles.
STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway.
The Sec1p homologue Vps45p binds to the syntaxin Tlg2p.
The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport.
Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles.
Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae.
Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae.
Casein kinase II of yeast contains two distinct alpha polypeptides and an unusually large beta subunit.
Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II.
Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein.
Restriction of histone gene transcription to S phase by phosphorylation of a chromatin boundary protein.
A family of small coiled-coil-forming proteins functioning at the late endosome in yeast.
A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase.
CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins.
Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting.
Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans.
Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4.
Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae.
Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I.
Multiple copies of PBS2, MHP1 or LRE1 produce glucanase resistance and other cell wall effects in Saccharomyces cerevisiae.
Pbn1p: an essential endoplasmic reticulum membrane protein required for protein processing in the endoplasmic reticulum of budding yeast.
The PBN1 gene of Saccharomyces cerevisiae: an essential gene that is required for the post-translational processing of the protease B precursor.
Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival.
The YCR079w gene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae.
The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast.
A single amino acid substitution in yeast eIF-5A results in mRNA stabilization.
Complex formation between deoxyhypusine synthase and its protein substrate, the eukaryotic translation initiation factor 5A (eIF5A) precursor.
Crystal structure of Hypusine-containing translation factor eIF5A bound to a rotated eukaryotic ribosome.
Determination and mutational analysis of the phosphorylation site in the hypusine-containing protein Hyp2p.
Dimerization of the yeast eukaryotic translation initiation factor 5A requires hypusine and is RNA dependent.
Evidence for a negative cooperativity between eIF5A and eEF2 on binding to the ribosome.
Fertility and polarized cell growth depends on eIF5A for translation of polyproline-rich formins in Saccharomyces cerevisiae.
Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling.
Mapping eIF5A binding sites for Dys1 and Lia1: in vivo evidence for regulation of eIF5A hypusination.
Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.
Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression.
Sequence determination of cDNA encoding yeast cycloheximide sensitivity factor (CH-SF) and its plausible role at a first peptide bond formation step in the initiation process of protein synthesis.
Structural modeling and mutational analysis of yeast eukaryotic translation initiation factor 5A reveal new critical residues and reinforce its involvement in protein synthesis.
Structure of the hypusinylated eukaryotic translation factor eIF-5A bound to the ribosome.
Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex.
Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine in Saccharomyces cerevisiae.
eIF5A binds to translational machinery components and affects translation in yeast.
eIF5A functions globally in translation elongation and termination.
eIF5A promotes translation of polyproline motifs.
Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae.
Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a 43.7 kb fragment of chromosome XII including an open reading frame homologous to the human cystic fibrosis transmembrane conductance regulator protein CFTR.
Cloning and analysis of the nuclear genes for two mitochondrial ribosomal proteins in yeast.
Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae.
Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP7, a protein of the large subunit of the mitochondrial ribosome.
Transport of mitoribosomal proteins, YmL13 and MRP7, into isolated mitochondria of Saccharomyces cerevisiae.
PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.
Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiae.
The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine.
A genome-wide screen for genes affecting eisosomes reveals Nce102 function in sphingolipid signaling.
A new pathway for protein export in Saccharomyces cerevisiae.
C terminus of Nce102 determines the structure and function of microdomains in the Saccharomyces cerevisiae plasma membrane.
Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast.
Nonclassical export pathway: overexpression of NCE102 reduces protein and DNA damage and prolongs lifespan in an SGS1 deficient Saccharomyces cerevisiae.
Plasma membrane microdomains regulate turnover of transport proteins in yeast.
Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae.
High level expression and characterization of the mitochondrial citrate transport protein from the yeast Saccharomyces cerevisiae.
A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.
A generic protein purification method for protein complex characterization and proteome exploration.
A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle.
A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae.
Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae.
Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae.
Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae.
Molecular characterization of carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria in Saccharomyces cerevisiae and identification of a plasma membrane carnitine transporter, Agp2p.
YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic reticulum of Saccharomyces cerevisiae.
Crp1p, a new cruciform DNA-binding protein in the yeast Saccharomyces cerevisiae.
Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins.
The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.
Control of cellular physiology by TM9 proteins in yeast and Dictyostelium.
Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal contents without changing vacuolar morphology.
Identification and classification of genes required for tolerance to freeze-thaw stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strains.
Identification of genes required for maximal tolerance to high-glucose concentrations, as those present in industrial alcoholic fermentation media, through a chemogenomics approach.
RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif.
Cross talk between sphingolipids and glycerophospholipids in the establishment of plasma membrane asymmetry.
Identification and characterization of a Saccharomyces cerevisiae gene, RSB1, involved in sphingoid long-chain base release.
Long chain base tolerance in Saccharomyces cerevisiae is induced by retrograde signals from the mitochondria.
Structural and biochemical characterization of the yeast HD domain containing protein YGK1 reveals a metal-dependent nucleoside 5'-monophosphatase.
Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae.
Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer.
Identification, mutational analysis, and coactivator requirements of two distinct transcriptional activation domains of the Saccharomyces cerevisiae Hap4 protein.
The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor.
Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex.
Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane.
Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum.
Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p.
SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum.
Isolation and characterization of CAJ1, a novel yeast homolog of dnaJ.
The evolutionary conserved BER1 gene is involved in microtubule stability in yeast.
FLX1 codes for a carrier protein involved in maintaining a proper balance of flavin nucleotides in yeast mitochondria.
A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA.
Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA.
Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae.
A conserved deubiquitinating enzyme uses intrinsically disordered regions to scaffold multiple protein interaction sites.
A proteomic strategy for gaining insights into protein sumoylation in yeast.
DOT4 links silencing and cell growth in Saccharomyces cerevisiae.
Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae.
Identification of a member of a DNA-dependent ATPase family that causes interference with silencing.
Insertion site specificity of the transposon Tn3.
Separation-of-function mutants of yeast Ku80 reveal a Yku80p-Sir4p interaction involved in telomeric silencing.
Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3.
Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.
The molecular biology of the SIR proteins.
Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway.
Regulation of G0 entry by the Pho80-Pho85 cyclin-CDK complex.
Saccharomyces cerevisiae cAMP-dependent protein kinase controls entry into stationary phase through the Rim15p protein kinase.
Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Rim15p.
Dbp7p, a putative ATP-dependent RNA helicase from Saccharomyces cerevisiae, is required for 60S ribosomal subunit assembly.
The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene.
The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p.
The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions.
Effect of 21 different nitrogen sources on global gene expression in the yeast Saccharomyces cerevisiae.
Global transcriptional and physiological responses of Saccharomyces cerevisiae to ammonium, L-alanine, or L-glutamine limitation.
Sequences of two adjacent genes, one (DAL2) encoding allantoicase and another (DCG1) sensitive to nitrogen-catabolite repression in Saccharomyces cerevisiae.
A novel yeast gene product, G4p1, with a specific affinity for quadruplex nucleic acids.
Arc1p is required for cytoplasmic confinement of synthetases and tRNA.
Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold.
The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanism.
The yeast inositol monophosphatase is a lithium- and sodium-sensitive enzyme encoded by a non-essential gene pair.
Yeast inositol mono- and trisphosphate levels are modulated by inositol monophosphatase activity and nutrients.
A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p.
Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import.
Pex9p is a new yeast peroxisomal import receptor for PTS1-containing proteins.
The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner.
Ubiquitination of the peroxisomal import receptor Pex5p.
Contribution of Are1p and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae.
Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltransferase.
Sterol esterification in yeast: a two-gene process.
Protein insertion into the mitochondrial inner membrane by a twin-pore translocase.
Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue.
Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA.
Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.
Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane.
An activation-independent role of transcription factors in insulator function.
General regulatory factors (GRFs) as genome partitioners.
REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb.
Swt1, a novel yeast protein, functions in transcription.
Coa2 is an assembly factor for yeast cytochrome C oxidase biogenesis that facilitates the maturation of cox1.
Structures of eukaryotic ribonucleotide reductase I define gemcitabine diphosphate binding and subunit assembly.
Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
The structural basis for peptidomimetic inhibition of eukaryotic ribonucleotide reductase: a conformationally flexible pharmacophore.
Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase.
Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex.
Yeast Sml1, a protein inhibitor of ribonucleotide reductase.
A conserved membrane-binding domain targets proteins to organelle contact sites.
An inducible ER-Golgi tether facilitates ceramide transport to alleviate lipotoxicity.
Yeast Svf1 binds ceramides and contributes to sphingolipid metabolism at the ER cis-Golgi interface.
Dxo1 is a new type of eukaryotic enzyme with both decapping and 5'-3' exoribonuclease activity.
CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions.
Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p.
Metalloregulation of yeast membrane steroid receptor homologs.
Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element.
Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions.
A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae.
A heterodimer of the Zn2Cys6 transcription factors Pip2p and Oaf1p controls induction of genes encoding peroxisomal proteins in Saccharomyces cerevisiae.
Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p. Different roles in fatty acid induction of beta-oxidation in Saccharomyces cerevisiae.
Purification, identification, and properties of a Saccharomyces cerevisiae oleate-activated upstream activating sequence-binding protein that is involved in the activation of POX1.
Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE.
Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions.
Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast.
PRE5 and PRE6, the last missing genes encoding 20S proteasome subunits from yeast? Indication for a set of 14 different subunits in the eukaryotic proteasome core.
Regulation of the yeast triacylglycerol lipase Tgl3p by formation of nonpolar lipids.
YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae.
Identification of the heme axial ligands in the cytochrome b562 of the Saccharomyces cerevisiae succinate dehydrogenase.
Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase.
The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductase.
The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studies.
The quinone-binding sites of the Saccharomyces cerevisiae succinate-ubiquinone oxidoreductase.
The role of Sdh4p Tyr-89 in ubiquinone reduction by the Saccharomyces cerevisiae succinate dehydrogenase.
Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae.
A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome.
Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi.
Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth.
Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast.
Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.
VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation.
A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing.
New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation.
Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery.
Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing.
A folding transition and novel zinc finger accessory domain in the transcription factor ADR1.
A mutation outside the two zinc fingers of ADR1 can suppress defects in either finger.
ADR1a, a zinc finger peptide, exists in two folded conformations.
ADR1c mutations enhance the ability of ADR1 to activate transcription by a mechanism that is independent of effects on cyclic AMP-dependent protein kinase phosphorylation of Ser-230.
Alanine scanning site-directed mutagenesis of the zinc fingers of transcription factor ADR1: residues that contact DNA and that transactivate.
Mutations in the zinc fingers of ADR1 that change the specificity of DNA binding and transactivation.
Mutations in the zinc-finger region of the yeast regulatory protein ADR1 affect both DNA binding and transcriptional activation.
Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA.
Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function.
Zinc-dependent structure of a single-finger domain of yeast ADR1.
A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae.
Cdc13 cooperates with the yeast Ku proteins and Stn1 to regulate telomerase recruitment.
HDF2, the second subunit of the Ku homologue from Saccharomyces cerevisiae.
Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance.
Involvement of replicative polymerases, Tel1p, Mec1p, Cdc13p, and the Ku complex in telomere-telomere recombination.
Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends.
Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres.
Sequence diversity, reproductive isolation and species concepts in Saccharomyces.
Telomerase, Ku, and telomeric silencing in Saccharomyces cerevisiae.
Telomere maintenance is dependent on activities required for end repair of double-strand breaks.
The DNA repair protein yKu80 regulates the function of recombination enhancer during yeast mating type switching.
The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini.
The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining.
The yeast Ku heterodimer is essential for protection of the telomere against nucleolytic and recombinational activities.
Yeast Ku as a regulator of chromosomal DNA end structure.
Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex.
Molecular architecture of a kinetochore-microtubule attachment site.
The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement.
The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends.
The mitotic spindle is required for loading of the DASH complex onto the kinetochore.
The yeast DASH complex forms closed rings on microtubules.
Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae.
Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae.
Characterization of an ERAD gene as VPS30/ATG6 reveals two alternative and functionally distinct protein quality control pathways: one for soluble Z variant of human alpha-1 proteinase inhibitor (A1PiZ) and another for aggregates of A1PiZ.
ER-associated complexes (ERACs) containing aggregated cystic fibrosis transmembrane conductance regulator (CFTR) are degraded by autophagy.
Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products.
O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae.
Overexpression of autophagy-related genes inhibits yeast filamentous growth.
Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy.
Structure of the novel C-terminal domain of vacuolar protein sorting 30/autophagy-related protein 6 and its specific role in autophagy.
The role of autophagy in mitochondria maintenance: characterization of mitochondrial functions in autophagy-deficient S. cerevisiae strains.
A new essential gene located on Saccharomyces cerevisiae chromosome IX.
Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion.
Molecular architecture of SMC proteins and the yeast cohesin complex.
Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae.
A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage.
Bdf1, a yeast chromosomal protein required for sporulation.
Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2.
Bromodomain factor 1 (Bdf1) protein interacts with histones.
Bromodomain factor 1 corresponds to a missing piece of yeast TFIID.
Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries.
Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation.
Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA.
Multiple bromodomain genes are involved in restricting the spread of heterochromatic silencing at the Saccharomyces cerevisiae HMR-tRNA boundary.
Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1.
The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast.
The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs.
A multitude of suppressors of group II intron-splicing defects in yeast.
Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machinery.
The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome.
A TOG protein confers tension sensitivity to kinetochore-microtubule attachments.
A TOG:alphabeta-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase.
A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.
Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.
Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.
Stu2 promotes mitotic spindle elongation in anaphase.
Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency.
Stu2p binds tubulin and undergoes an open-to-closed conformational change.
Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body.
The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly.
The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames.
Analysis of the THR4 region on chromosome III of the yeast Saccharomyces cerevisiae.
Nucleotide sequence of the yeast THR4 gene encoding threonine synthase.
Btn3 is a negative regulator of Btn2-mediated endosomal protein trafficking and prion curing in yeast.
Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae.
Interaction with Btn2p is required for localization of Rsglp: Btn2p-mediated changes in arginine uptake in Saccharomyces cerevisiae.
The yeast model for Batten disease: a role for Btn2p in the trafficking of the Golgi-associated vesicular targeting protein, Yif1p.
The yeast model for Batten disease: mutations in BTN1, BTN2, and HSP30 alter pH homeostasis.
MPV17 encodes an inner mitochondrial membrane protein and is mutated in infantile hepatic mitochondrial DNA depletion.
SYM1 is the stress-induced Saccharomyces cerevisiae ortholog of the mammalian kidney disease gene Mpv17 and is required for ethanol metabolism and tolerance during heat shock.
Discovery of a rapidly evolving yeast defense factor, KTD1, against the secreted killer toxin K28.
A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1.
Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1.
Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint.
Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions.
Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p.
Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3.
Requirement for ATP by the DNA damage checkpoint clamp loader.
Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway.
The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae.
Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint.
Yeast checkpoint genes in DNA damage processing: implications for repair and arrest.
Functional characterization of the Saccharomyces cerevisiae VHS3 gene. A regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions.
Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae.
Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis.
The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence.
ARD1 and NAT1 proteins form a complex that has N-terminal acetyltransferase activity.
Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast.
Molecular cloning and sequencing of a cDNA encoding N alpha-acetyltransferase from Saccharomyces cerevisiae.
The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides.
Analysis of a yeast nuclear gene involved in the maturation of mitochondrial pre-messenger RNA of the cytochrome oxidase subunit I.
Analysis of the MSS51 region on chromosome XII of Saccharomyces cerevisiae.
COX24 codes for a mitochondrial protein required for processing of the COX1 transcript.
Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways.
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation.
Novel G-protein complex whose requirement is linked to the translational status of the cell.
Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.
The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames.
Collided ribosomes form a unique structural interface to induce Hel2-driven quality control pathways.
MOT2 encodes a negative regulator of gene expression that affects basal expression of pheromone-responsive genes in Saccharomyces cerevisiae.
Molecular characterization of SIG1, a Saccharomyces cerevisiae gene involved in negative regulation of G-protein-mediated signal transduction.
NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization.
Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex.
The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5.
The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively.
The yeast MOT2 gene encodes a putative zinc finger protein that serves as a global negative regulator affecting expression of several categories of genes, including mating-pheromone-responsive genes.
Assembly of the mitochondrial membrane system. MRP1 and MRP2, two yeast nuclear genes coding for mitochondrial ribosomal proteins.
Discoordinate expression of the yeast mitochondrial ribosomal protein MRP1.
The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism.
Activation of the checkpoint kinase Rad53 by the phosphatidyl inositol kinase-like kinase Mec1.
Characterization of mec1 kinase-deficient mutants and of new hypomorphic mec1 alleles impairing subsets of the DNA damage response pathway.
LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae.
MEC3, MEC1, and DDC2 are essential components of a telomere checkpoint pathway required for cell cycle arrest during senescence in Saccharomyces cerevisiae.
Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae.
Role of the C-terminus of Mec1 checkpoint kinase in its localization to sites of DNA damage.
Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.
The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast.
Two checkpoint complexes are independently recruited to sites of DNA damage in vivo.
A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation.
Degradation of a cytosolic protein requires endoplasmic reticulum-associated degradation machinery.
Inactivation of SSM4, a new Saccharomyces cerevisiae gene, suppresses mRNA instability due to RNA14 mutations.
Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways.
Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI).
Membrane-bound Ubx2 recruits Cdc48 to ubiquitin ligases and their substrates to ensure efficient ER-associated protein degradation.
Msp1 clears mistargeted proteins by facilitating their transfer from mitochondria to the ER.
N-terminal acetylation of cellular proteins creates specific degradation signals.
Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase.
Ubiquitin chain elongation enzyme Ufd2 regulates a subset of Doa10 substrates.
Ubx2 links the Cdc48 complex to ER-associated protein degradation.
Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.
Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces.
Model for eukaryotic tail-anchored protein binding based on the structure of Get3.
Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex.
Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.
Structural insight into the membrane insertion of tail-anchored proteins by Get3.
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
The GET complex mediates insertion of tail-anchored proteins into the ER membrane.
The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance.
The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.
The crystal structures of yeast Get3 suggest a mechanism for tail-anchored protein membrane insertion.
The mechanism of membrane-associated steps in tail-anchored protein insertion.
The structural basis of tail-anchored membrane protein recognition by Get3.
The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol.
WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane.
Analysis of oligomerization properties of heme a synthase provides insights into its function in eukaryotes.
COX15 codes for a mitochondrial protein essential for the assembly of yeast cytochrome oxidase.
Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O.
MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C.
Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae.
Dual lipid modification motifs in G(alpha) and G(gamma) subunits are required for full activity of the pheromone response pathway in Saccharomyces cerevisiae.
Dual lipid modification of the yeast gamma subunit Ste18p determines membrane localization of Gbetagamma.
Site-directed mutations altering the CAAX box of Ste18, the yeast pheromone-response pathway G gamma subunit.
The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein.
A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex.
A yeast gene that is essential for release from glucose repression encodes a protein kinase.
ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase.
An inhibited conformation for the protein kinase domain of the Saccharomyces cerevisiae AMPK homolog Snf1.
Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1.
Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1.
Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae.
Glucose repression of yeast mitochondrial transcription: kinetics of derepression and role of nuclear genes.
Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism.
High-affinity glucose transport in Saccharomyces cerevisiae is under general glucose repression control.
Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms.
Key role of Ser562/661 in Snf1-dependent regulation of Cat8p in Saccharomyces cerevisiae and Kluyveromyces lactis.
Mammalian AMP-activated protein kinase shares structural and functional homology with the catalytic domain of yeast Snf1 protein kinase.
Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase.
Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein.
N-terminal mutations modulate yeast SNF1 protein kinase function.
PAS kinase is activated by direct SNF1-dependent phosphorylation and mediates inhibition of TORC1 through the phosphorylation and activation of Pbp1.
Protein kinase Snf1 is involved in the proper regulation of the unfolded protein response in Saccharomyces cerevisiae.
Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.
Snf1 phosphorylates adenylate cyclase and negatively regulates protein kinase A-dependent transcription in Saccharomyces cerevisiae.
Snf1-dependent transcription confers glucose-induced decay upon the mRNA product.
Structural insight into the autoinhibition mechanism of AMP-activated protein kinase.
Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family.
Structure and expression of the SNF1 gene of Saccharomyces cerevisiae.
Subunits of the Snf1 kinase heterotrimer show interdependence for association and activity.
Sumoylation regulates the SNF1 protein kinase.
The CCR1 (SNF1) and SCH9 protein kinases act independently of cAMP-dependent protein kinase and the transcriptional activator ADR1 in controlling yeast ADH2 expression.
The SNF1 kinase ubiquitin-associated domain restrains its activation, activity, and the yeast life span.
The Saccharomyces cerevisiae AMPK, Snf1, negatively regulates the Hog1 MAPK pathway in ER stress response.
The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex.
Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response.
Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast.
Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae.
Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae.
In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation.
Yeast epsins contain an essential N-terminal ENTH domain, bind clathrin and are required for endocytosis.
Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites.
Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae.
The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames.
GUD1 (YDL238c) encodes Saccharomyces cerevisiae guanine deaminase, an enzyme expressed during post-diauxic growth.
TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function.
COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression.
COMPASS: a complex of proteins associated with a trithorax-related SET domain protein.
The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4.
Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae.
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.
Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins.
Nucleotide sequence of the LYS2 gene of Saccharomyces cerevisiae: homology to Bacillus brevis tyrocidine synthetase 1.
A yeast gene coding for a putative protein kinase homologous to cdc25 suppressing protein kinase.
Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.
PDK1 homologs activate the Pkc1-mitogen-activated protein kinase pathway in yeast.
Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation.
The Mnd1 protein forms a complex with Hop2 to promote homologous chromosome pairing and meiotic double-strand break repair.
The meiosis-specific Hop2 protein of S. cerevisiae ensures synapsis between homologous chromosomes.
The third exon of the budding yeast meiotic recombination gene HOP2 is required for calcium-dependent and recombinase Dmc1-specific stimulation of homologous strand assimilation.
PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.
Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance.
Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae.
Structure of Saccharomyces cerevisiae mitochondrial Qri7 in complex with AMP.
Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase.
RPH1 and GIS1 are damage-responsive repressors of PHR1.
Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins.
Exportin 1 (Crm1p) is an essential nuclear export factor.
Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1.
The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.
A regulatory factor, Fil1p, involved in derepression of the isocitrate lyase gene in Saccharomyces cerevisiae -- a possible mitochondrial protein necessary for protein synthesis in mitochondria.
Temperature-sensitive mutation in yeast mitochondrial ribosome recycling factor (RRF).
Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae.
A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p.
Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae.
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.
Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4.
F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex.
Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro.
Grr1 of Saccharomyces cerevisiae is connected to the ubiquitin proteolysis machinery through Skp1: coupling glucose sensing to gene expression and the cell cycle.
Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4.
Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1.
Regulated degradation of the transcription factor Gcn4.
SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene.
Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases.
The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast.
The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex.
Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene.
Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors.
Receptor-mediated endoproteolytic activation of two transcription factors in yeast.
Stp1p, Stp2p and Abf1p are involved in regulation of expression of the amino acid transporter gene BAP3 of Saccharomyces cerevisiae.
The N-terminal regulatory domain of Stp1p is modular and, fused to an artificial transcription factor, confers full Ssy1p-Ptr3p-Ssy5p sensor control.
Transcriptional profiling of extracellular amino acid sensing in Saccharomyces cerevisiae and the role of Stp1p and Stp2p.
Transcriptional regulation of the Saccharomyces cerevisiae amino acid permease gene BAP2.
ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p.
Characterization of a transcription factor involved in mother cell specific transcription of the yeast HO gene.
Interactions between Pho85 cyclin-dependent kinase complexes and the Swi5 transcription factor in budding yeast.
Solution structures of two zinc-finger domains from SWI5 obtained using two-dimensional 1H nuclear magnetic resonance spectroscopy. A zinc-finger structure with a third strand of beta-sheet.
The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5.
Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA.
Characterization of Gtf1p, the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase.
Mutations that increase the mitotic stability of minichromosomes in yeast: characterization of RAR1.
Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
The function and properties of the Azf1 transcriptional regulator change with growth conditions in Saccharomyces cerevisiae.
CHL15 -- a new gene controlling the replication of chromosomes in Saccharomycetes yeast: cloning, physical mapping, sequencing, and sequence analysis.
CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae.
Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo.
Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7.
A comparison of yeast ribosomal protein gene DNA sequences.
Structure of the ribosome-bound cricket paralysis virus IRES RNA.
Synchronizing nuclear import of ribosomal proteins with ribosome assembly.
Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes.
Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae.
Rapid telomere movement in meiotic prophase is promoted by NDJ1, MPS3, and CSM4 and is modulated by recombination.
CDC39, an essential nuclear protein that negatively regulates transcription and differentially affects the constitutive and inducible HIS3 promoters.
A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae.
The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.
Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.
Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae.
NTE1-encoded phosphatidylcholine phospholipase B regulates transcription of phospholipid biosynthetic genes.
Neuropathy target esterase and its yeast homologue degrade phosphatidylcholine to glycerophosphocholine in living cells.
Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p.
Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae.
PKR1 encodes an assembly factor for the yeast V-type ATPase.
A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits.
Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling.
Formation and nuclear export of preribosomes are functionally linked to the small-ubiquitin-related modifier pathway.
High-definition macromolecular composition of yeast RNA-processing complexes.
Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p.
Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits.
Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.
A unique class of conditional sir2 mutants displays distinct silencing defects in Saccharomyces cerevisiae.
An enzymatic activity in the yeast Sir2 protein that is essential for gene silencing.
Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing.
Characterization of two genes required for the position-effect control of yeast mating-type genes.
Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci.
Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex.
Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogues and 32P-NAD.
Localization of Sir2p: the nucleolus as a compartment for silent information regulators.
Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity.
Restoration of silencing in Saccharomyces cerevisiae by tethering of a novel Sir2-interacting protein, Esc8.
Silenced chromatin is permissive to activator binding and PIC recruitment.
Silencing factors participate in DNA repair and recombination in Saccharomyces cerevisiae.
Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3.
Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2.
Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan.
Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding.
Substrate specificity and kinetic mechanism of the Sir2 family of NAD+-dependent histone/protein deacetylases.
Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.
Two paralogs involved in transcriptional silencing that antagonistically control yeast life span.
A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-ATPase.
A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant.
Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae.
Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation.
Molecular cloning of CIF1, a yeast gene necessary for growth on glucose.
Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae.
Purification of trehalose synthase from baker's yeast. Its temperature-dependent activation by fructose 6-phosphate and inhibition by phosphate.
cDNA cloning, sequence analysis of trehalose-6-phosphate synthase gene from Saccharomyces cerevisiae AS2.1416.
Cloning, molecular characterization, and expression of an endo-polygalacturonase-encoding gene from Saccharomyces cerevisiae IM1-8b.
Production and partial characterization of an endopolygalacturonase from Saccharomyces cerevisiae.
Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton.
The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex.
Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.
Yeast Rsp5 ubiquitin ligase affects the actin cytoskeleton in vivo and in vitro.
Low molecular weight proteins: a challenge for post-genomic research.
Sorting of proteins into multivesicular bodies: ubiquitin-dependent and -independent targeting.
A cyclic form of N6-threonylcarbamoyladenosine as a widely distributed tRNA hypermodification.
A novel cloning strategy reveals the gene for the yeast homologue to Escherichia coli ribosomal protein S12.
The sequence of a 23.4 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals CLB6, SPT6, RP28A and NUP57 genes, a Ty3 element and 11 new open reading frames.
Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen.
Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression.
Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I.
The ankyrin repeat-containing protein Akr1p is required for the endocytosis of yeast pheromone receptors.
Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase.
Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames.
Suppression of a myosin defect by a kinesin-related gene.
Functional domains of the rsp5 ubiquitin-protein ligase.
RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6.
Characterization of two genes, ARGRI and ARGRIII required for specific regulation of arginine metabolism in yeast.
Determination of the DNA-binding sequences of ARGR proteins to arginine anabolic and catabolic promoters.
Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae.
In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter.
Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability.
Regulation of arginine metabolism in Saccharomyces cerevisiae: expression of the three ARGR regulatory genes and cellular localization of their products.
Characteristics of galactose transport in Saccharomyces cerevisiae cells and reconstituted lipid vesicles.
Induction of enzymes of the galactose pathway in mutants of Saccharomyces cerevisiae.
Kinetic analysis of yeast galactokinase: implications for transcriptional activation of the GAL genes.
Molecular structure of Saccharomyces cerevisiae Gal1p, a bifunctional galactokinase and transcriptional inducer.
Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon.
Purification and properties of galactokinase from Saccharomyces cerevisiae.
Sequence of the Saccharomyces GAL region and its transcription in vivo.
Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
Specific DNA binding of GAL4, a positive regulatory protein of yeast.
The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae.
Evidence for preferential multiplication of the internal unit in tandem repeats of the mating factor alpha genes in Saccharomyces yeasts.
Identification of sequence elements that confer cell-type-specific control of MF alpha 1 expression in Saccharomyces cerevisiae.
Purification and amino acid sequence of mating factor from Saccharomyces cerevisiae.
Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
Unconventional genomic architecture in the budding yeast Saccharomyces cerevisiae masks the nested antisense gene NAG1.
A conserved family of calcineurin regulators.
A new gene family including DSCR1 (Down syndrome candidate region 1) and ZAKI-4: characterization from yeast to human and identification of DSCR1-like 2, a novel human member (DSCR1L2).
A role for intersubunit interactions in maintaining SAGA deubiquitinating module structure and activity.
Cluster analysis of mass spectrometry data reveals a novel component of SAGA.
H2B ubiquitin protease Ubp8 and Sgf11 constitute a discrete functional module within the Saccharomyces cerevisiae SAGA complex.
Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits.
Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module.
Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1.
Structural insights into the assembly and function of the SAGA deubiquitinating module.
The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex.
The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11.
Ubp8p, a histone deubiquitinase whose association with SAGA is mediated by Sgf11p, differentially regulates lysine 4 methylation of histone H3 in vivo.
Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export.
Identification of the yeast nuclear gene for the mitochondrial homologue of bacterial ribosomal protein L16.
Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired.
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins.
Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.
Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51.
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.
Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.
High-resolution DNA-binding specificity analysis of yeast transcription factors.
Identification of Coq11, a new coenzyme Q biosynthetic protein in the CoQ-synthome in Saccharomyces cerevisiae.
Role of the yeast Gin4p protein kinase in septin assembly and the relationship between septin assembly and septin function.
A genome-wide immunodetection screen in S. cerevisiae uncovers novel genes involved in lysosomal vacuole function and morphology.
Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus.
Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth.
Cooperation of two mRNA-binding proteins drives metabolic adaptation to iron deficiency.
Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.
The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases.
Functional analysis of six novel ORFs on the left arm of chromosome XII of Saccharomyces cerevisiae reveals three of them responding to S-starvation.
A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant.
The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome.
The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes.
Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex.
Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base.
cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9).
Asymmetric structure of the yeast F1 ATPase in the absence of bound nucleotides.
Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants.
Molecular architecture of the rotary motor in ATP synthase.
Mutations in the mitochondrial ATP synthase gamma subunit suppress a slow-growth phenotype of yme1 yeast lacking mitochondrial DNA.
Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase.
Studies on the ATP3 gene of Saccharomyces cerevisiae: presence of two closely linked copies, ATP3a and ATP3b, on the right arm of chromosome II.
Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins.
Three cell wall mannoproteins facilitate the uptake of iron in Saccharomyces cerevisiae.
Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
Large-scale prediction of Saccharomyces cerevisiae gene function using overlapping transcriptional clusters.
The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes.
A distinct nuclear import pathway used by ribosomal proteins.
Importin-beta-like nuclear transport receptors.
Importin/karyopherin protein family members required for mRNA export from the nucleus.
Intersection of the Kap123p-mediated nuclear import and ribosome export pathways.
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
Nuclear import of the yeast AP-1-like transcription factor Yap1p is mediated by transport receptor Pse1p, and this import step is not affected by oxidative stress.
Pathways mediating the nuclear import of histones H3 and H4 in yeast.
Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p.
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
ATF/CREB sites present in sub-telomeric regions of Saccharomyces cerevisiae chromosomes are part of promoters and act as UAS/URS of highly conserved COS genes.
Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress.
Barrier proteins remodel and modify chromatin to restrict silenced domains.
The nature of the nitrogen source added to nitrogen depleted vinifications conducted by a Saccharomyces cerevisiae strain in synthetic must affects gene expression and the levels of several volatile compounds.
The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein.
The APC11 RING-H2 finger mediates E2-dependent ubiquitination.
Novel E3 ubiquitin ligases that regulate histone protein levels in the budding yeast Saccharomyces cerevisiae.
Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36.
Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames.
The yeast Snt2 protein coordinates the transcriptional response to hydrogen peroxide-mediated oxidative stress.
A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis: acetylglutamate synthase activity requires complex formation with acetylglutamate kinase.
Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes.
Regulation of activity and synthesis of N-acetylglutamate synthase from Saccharomyces cerevisiae.
Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8.
Evolution of gene families: the multidrug resistance transporter genes in five related yeast species.
Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae.
Multiprotein transcription factor UAF interacts with the upstream element of the yeast RNA polymerase I promoter and forms a stable preinitiation complex.
Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription.
Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene.
Purification and properties of the alkylation repair DNA glycosylase encoded the MAG gene from Saccharomyces cerevisiae.
Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology to the AlkA glycosylase of E. coli and is induced in response to DNA alkylation damage.
Two distinct genes encode small isoproteolipids affecting plasma membrane H(+)-ATPase activity of Saccharomyces cerevisiae.
Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae.
Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae.
Genomewide identification of Sko1 target promoters reveals a regulatory network that operates in response to osmotic stress in Saccharomyces cerevisiae.
7The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex.
A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae.
A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.
Arginine methylation facilitates the nuclear export of hnRNP proteins.
Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast.
Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae.
Eukaryote-specific domains in translation initiation factors: implications for translation regulation and evolution of the translation system.
GCR3 encodes an acidic protein that is required for expression of glycolytic genes in Saccharomyces cerevisiae.
Genetic and physical interactions involving the yeast nuclear cap-binding complex.
Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex.
Importin provides a link between nuclear protein import and U snRNA export.
Mutations in GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, suppress the temperature-sensitive growth of hpr1 mutants.
Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts.
Subunit architecture of multimeric complexes isolated directly from cells.
The interaction of the cap-binding complex (CBC) with eIF4G is dispensable for translation in yeast.
The role of nuclear cap binding protein Cbc1p of yeast in mRNA termination and degradation.
The yeast nuclear cap binding complex can interact with translation factor eIF4G and mediate translation initiation.
A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB.
A yeast TFIIB-related factor involved in RNA polymerase III transcription.
Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes.
PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB.
A novel nucleolar G-protein conserved in eukaryotes.
Sequential protein association with nascent 60S ribosomal particles.
Active site determination of yeast geranylgeranyl protein transferase type I expressed in Escherichia coli.
CDC43 and RAM2 encode the polypeptide subunits of a yeast type I protein geranylgeranyltransferase.
Characterization of recombinant human farnesyl-protein transferase: cloning, expression, farnesyl diphosphate binding, and functional homology with yeast prenyl-protein transferases.
Genetic study of the role of calcium ions in the cell division cycle of Saccharomyces cerevisiae: a calcium-dependent mutant and its trifluoperazine-dependent pseudorevertants.
Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Saccharomyces cerevisiae.
Mutational analysis of the beta-subunit of yeast geranylgeranyl transferase I.
Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product.
Structural homology among mammalian and Saccharomyces cerevisiae isoprenyl-protein transferases.
Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing.
Yeast protein geranylgeranyltransferase type-I: overproduction, purification, and characterization.
Yeast protein geranylgeranyltransferase type-I: steady-state kinetics and substrate binding.
A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.
Unifying fluorescence microscopy and mass spectrometry for studying protein complexes in cells.
A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae.
Crystallization and preliminary X-ray analysis of CTP:phosphoethanolamine cytidylyltransferase (ECT) from Saccharomyces cerevisiae.
Isolation and characterization of ECT1 gene encoding CTP: phosphoethanolamine cytidylyltransferase of Saccharomyces cerevisiae.
Manipulation of major membrane lipid synthesis and its effects on sporulation in Saccharomyces cerevisiae.
Respiratory deficiency mediates the regulation of CHO1-encoded phosphatidylserine synthase by mRNA stability in Saccharomyces cerevisiae.
Screening of genes involved in isooctane tolerance in Saccharomyces cerevisiae by using mRNA differential display.
SEC6 encodes an 85 kDa soluble protein required for exocytosis in yeast.
The structure of the exocyst subunit Sec6p defines a conserved architecture with diverse roles.
Role of the nuclear transport factor p10 in nuclear import.
SEC3 mutations are synthetically lethal with profilin mutations and cause defects in diploid-specific bud-site selection.
Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats.
Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae.
The ALD6 gene of Saccharomyces cerevisiae encodes a cytosolic, Mg(2+)-activated acetaldehyde dehydrogenase.
NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae.
Structural comparison of yeast ribosomal protein genes.
Suppression of sdh1 mutations by the SDH1b gene of Saccharomyces cerevisiae.
Genes of succinyl-CoA ligase from Saccharomyces cerevisiae.
Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.
Autophosphorylation-inactivation site of hexokinase 2 in Saccharomyces cerevisiae.
Hexokinase 2 from Saccharomyces cerevisiae: regulation of oligomeric structure by in vivo phosphorylation at serine-14.
Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae.
Identification, cloning and sequence determination of the genes specifying hexokinase A and B from yeast.
In vivo phosphorylation site of hexokinase 2 in Saccharomyces cerevisiae.
Physiological role of glucose-phosphorylating enzymes in Saccharomyces cerevisiae.
Sequencing a protein by X-ray crystallography. II. Refinement of yeast hexokinase B co-ordinates and sequence at 2.1-A resolution.
The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression.
Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae.
The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping.
Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease.
ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes.
Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV.
Structures of yeast ARF2 and ARL1: distinct roles for the N-terminus in the structure and function of ARF family GTPases.
Cloning, primary structure, and regulation of the HIS7 gene encoding a bifunctional glutamine amidotransferase: cyclase from Saccharomyces cerevisiae.
Crystal structure of imidazole glycerol phosphate synthase: a tunnel through a (beta/alpha)8 barrel joins two active sites.
Substrate-induced changes in the ammonia channel for imidazole glycerol phosphate synthase.
Toward understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerolphosphate synthase: crystal structures of a ternary complex and the free enzyme.
Isolation, overexpression and disruption of a Saccharomyces cerevisiae YNK gene encoding nucleoside diphosphate kinase.
Kinetic studies of yeast nucleoside diphosphate kinase.
Nucleoside diphosphate kinase of Saccharomyces cerevisiae, Ynk1p: localization to the mitochondrial intermembrane space.
Nucleoside triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 3. Subunit structure of the crystalline enzyme from brewers' yeast.
Saccharomyces cerevisiae nucleoside-diphosphate kinase: purification, characterization, and substrate specificity.
Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins.
Structure of Ynk1 from the yeast Saccharomyces cerevisiae.
The nucleoside diphosphate kinase from mimivirus: a peculiar affinity for deoxypyrimidine nucleotides.
YNK1, the yeast homolog of human metastasis suppressor NM23, is required for repair of UV radiation- and etoposide-induced DNA damage.
A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex.
Nucleotide-induced conformational changes in the Saccharomyces cerevisiae SR protein kinase, Sky1p, revealed by X-ray crystallography.
The structure of Sky1p reveals a novel mechanism for constitutive activity.
A novel protein complex promoting formation of functional alpha- and gamma-tubulin.
Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system.
YKE2, a yeast nuclear gene encoding a protein showing homology to mouse KE2 and containing a putative leucine-zipper motif.
Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene.
The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches.
The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10.
Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component.
Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.
A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis.
A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast.
ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase.
Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall.
Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae.
Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi.
MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast.
Vps13-like proteins provide phosphatidylethanolamine for GPI anchor synthesis in the ER.
Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily.
Phenylalanine- and tyrosine-auxotrophic mutants of Saccharomyces cerevisiae impaired in transamination.
Tryptophan degradation in Saccharomyces cerevisiae: characterization of two aromatic aminotransferases.
Primary structure and function of a second essential member of the heterooligomeric TCP1 chaperonin complex of yeast, TCP1 beta.
Two yeast genes with similarity to TCP-1 are required for microtubule and actin function in vivo.
Composition and functional characterization of yeast 66S ribosome assembly intermediates.
In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.
Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation.
Rrp15p, a novel component of pre-ribosomal particles required for 60S ribosome subunit maturation.
Structural Analysis Reveals Features of Ribosome Assembly Factor Nsa1/WDR74 Important for Localization and Interaction with Rix7/NVL2.
Yeast mitochondrial F1F0-ATPase exists as a dimer: identification of three dimer-specific subunits.
Roles of multiple glucose transporters in Saccharomyces cerevisiae.
A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.
Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding.
Functional analysis of Met4, a yeast transcriptional activator responsive to S-adenosylmethionine.
MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae.
Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA.
The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs.
The general amino acid control regulates MET4, which encodes a methionine-pathway-specific transcriptional activator of Saccharomyces cerevisiae.
The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames.
Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme.
Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV.
Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis.
Screening and characterization of transposon-insertion mutants in a pseudohyphal strain of Saccharomyces cerevisiae.
Transcriptional profiling of Saccharomyces cerevisiae cells under adhesion-inducing conditions.
A bipartite yeast SSRP1 analog comprised of Pob3 and Nhp6 proteins modulates transcription.
CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus.
Characterization of the CP complex, an abundant dimer of Cdc68 and Pob3 proteins that regulates yeast transcriptional activation and chromatin repression.
Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure.
Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes.
Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae.
Spt16 and Pob3 of Saccharomyces cerevisiae form an essential, abundant heterodimer that is nuclear, chromatin-associated, and copurifies with DNA polymerase alpha.
Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN.
Structural features of nucleosomes reorganized by yeast FACT and its HMG box component, Nhp6.
The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.
The Paf1 complex physically and functionally associates with transcription elongation factors in vivo.
The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein.
The yeast FACT complex has a role in transcriptional initiation.
The yeast protein complex containing cdc68 and pob3 mediates core-promoter repression through the cdc68 N-terminal domain.
Transcription elongation factors repress transcription initiation from cryptic sites.
Transitions in RNA polymerase II elongation complexes at the 3' ends of genes.
Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges.
Eps1, a novel PDI-related protein involved in ER quality control in yeast.
Substrate recognition in ER-associated degradation mediated by Eps1, a member of the protein disulfide isomerase family.
Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane.
Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis.
ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression.
NuA4 subunit Yng2 function in intra-S-phase DNA damage response.
Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex.
Three yeast proteins related to the human candidate tumor suppressor p33(ING1) are associated with histone acetyltransferase activities.
Yng2p-dependent NuA4 histone H4 acetylation activity is required for mitotic and meiotic progression.
Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.
PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels.
Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation.
Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.
A two-component system that regulates an osmosensing MAP kinase cascade in yeast.
Crystal structure of the Rad3/XPD regulatory domain of Ssl1/p44.
Different forms of TFIIH for transcription and DNA repair: holo-TFIIH and a nucleotide excision repairosome.
Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair.
RNA polymerase transcription factor IIH holoenzyme from yeast.
Reconstitution of TFIIH and requirement of its DNA helicase subunits, Rad3 and Rad25, in the incision step of nucleotide excision repair.
Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.
SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast.
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin.
The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1.
The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain.
The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae.
Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners.
TOR controls transcriptional and translational programs via Sap-Sit4 protein phosphatase signaling effectors.
The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase.
The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity.
Alkaline response genes of Saccharomyces cerevisiae and their relationship to the RIM101 pathway.
Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall.
Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w.
Defect in cell wall integrity of the yeast Saccharomyces cerevisiae caused by a mutation of the GDP-mannose pyrophosphorylase gene VIG9.
Over-expression of S. cerevisiae G1 cyclins restores the viability of alg1 N-glycosylation mutants.
Saccharomyces cerevisiae VIG9 encodes GDP-mannose pyrophosphorylase, which is essential for protein glycosylation.
Global response of Saccharomyces cerevisiae to an alkylating agent.
The protein kinases of budding yeast: six score and more.
Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylglucosamine synthesis.
The crystal structures of Apo and complexed Saccharomyces cerevisiae GNA1 shed light on the catalytic mechanism of an amino-sugar N-acetyltransferase.
Comparative analysis of ribosome-associated adenosinetriphosphatase (ATPase) from pig liver and the ATPase of elongation factor 3 from Saccharomyces cerevisiae.
Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans.
Detection and analysis of translation elongation factor 3 genes from various yeasts.
Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress.
Functional interactions between yeast translation eukaryotic elongation factor (eEF) 1A and eEF3.
Identification and kinetic analysis of a functional homolog of elongation factor 3, YEF3 in Saccharomyces cerevisiae.
Isolation and characterization of the structural gene encoding elongation factor 3.
Overexpression of eukaryotic translation elongation factor 3 impairs Gcn2 protein activation.
Protein synthesis in yeast. I. Purification and properties of elongation factor 3 from Saccharomyces cerevisiae.
Protein synthesis in yeast. Structural and functional analysis of the gene encoding elongation factor 3.
Sequence analysis of the translational elongation factor 3 from Saccharomyces cerevisiae.
The N-terminus of eukaryotic translation elongation factor 3 interacts with 18 S rRNA and 80 S ribosomes.
The elongation factor 3 unique in higher fungi and essential for protein biosynthesis is an E site factor.
A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis.
Actin-induced hyperactivation of the Ras signaling pathway leads to apoptosis in Saccharomyces cerevisiae.
Characterization of a transport and detoxification pathway for the antitumour drug bleomycin in Saccharomyces cerevisiae.
EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae.
END3 and END4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.
End3p-mediated endocytosis is required for spore wall formation in Saccharomyces cerevisiae.
Endocytosis and degradation of the yeast uracil permease under adverse conditions.
High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.
Inositol transport in Saccharomyces cerevisiae is regulated by transcriptional and degradative endocytic mechanisms during the growth cycle that are distinct from inositol-induced regulation.
Metabolic instability and constitutive endocytosis of STE6, the a-factor transporter of Saccharomyces cerevisiae.
Morphology of the yeast endocytic pathway.
Multiple pathways regulate endocytic coat disassembly in Saccharomyces cerevisiae for optimal downstream trafficking.
Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis.
Pan1p, End3p, and Sla1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis.
Recognition specificity of individual EH domains of mammals and yeast.
Regulation of inositol transport in Saccharomyces cerevisiae involves inositol-induced changes in permease stability and endocytic degradation in the vacuole.
Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p.
Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p.
Scd5p mediates phosphoregulation of actin and endocytosis by the type 1 phosphatase Glc7p in yeast.
Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis.
The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast.
The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae.
The yeast actin-related protein Arp2p is required for the internalization step of endocytosis.
The allantoinase (DAL1) gene of Saccharomyces cerevisiae.
Saccharomyces cerevisiae MLF3/YNL074C gene, encoding a serine-rich protein of unknown function, determines the level of resistance to the novel immunosuppressive drug leflunomide.
Structure and evolution of a group of related aminoacyl-tRNA synthetases.
Arginine restriction induced by delta-N-(phosphonacetyl)-L-ornithine signals increased expression of HIS3, TRP5, CPA1, and CPA2 in Saccharomyces cerevisiae.
Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase.
Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo.
Sequence of the clathrin heavy chain from Saccharomyces cerevisiae and requirement of the COOH terminus for clathrin function.
The synaptojanin-like protein Inp53/Sjl3 functions with clathrin in a yeast TGN-to-endosome pathway distinct from the GGA protein-dependent pathway.
A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein.
A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis.
Characterization of Ccw7p cell wall proteins and the encoding genes of Saccharomyces cerevisiae wine yeast strains: relevance for flor formation.
Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae.
Increased mortality of Saccharomyces cerevisiae cell wall protein mutants.
Minisatellites in Saccharomyces cerevisiae genes encoding cell wall proteins: a new way towards wine strain characterisation.
Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p.
Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall.
Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae.
Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein.
The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants.
Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock.
Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall.
Gene-enzyme relationship in the sulfate assimilation pathway of Saccharomyces cerevisiae. Study of the 3'-phosphoadenylylsulfate reductase structural gene.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao
Analysis of phosphorylation of YJL084c, a yeast protein.
Binding to Elongin C inhibits degradation of interacting proteins in yeast.
Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo.
Regulation of the Pcl7-Pho85 cyclin-cdk complex by Pho81.
Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p.
The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p.
Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae.
Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis.
The CDC25 'Start' gene of Saccharomyces cerevisiae: sequencing of the active C-terminal fragment and regional homologies with rhodopsin and cytochrome P450.
The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras.
The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway.
Ethyl acetate production by the elusive alcohol acetyltransferase from yeast.
Mitochondrial protein turnover: role of the precursor intermediate peptidase Oct1 in protein stabilization.
Conversion of a cosubstrate to an inhibitor: phosphorylation mutants of nicotinic acid phosphoribosyltransferase.
The structure of a eukaryotic nicotinic acid phosphoribosyltransferase reveals structural heterogeneity among type II PRTases.
A 12.8 kb segment, on the right arm of chromosome II from Saccharomyces cerevisiae including part of the DUR1,2 gene, contains five putative new genes.
Involvement of the yeast hydrolase Ldh1p in lipid homeostasis.
The putative yeast hydrolase Ldh1p is localized to Lipid droplets.
Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends.
Ssu72 Is an RNA polymerase II CTD phosphatase.
Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast.
Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo.
A new glucose-repressible gene identified from the analysis of chromatin structure in deletion mutants of yeast SUC2 locus.
Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p.
Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo.
Purification and immunolocalization of the peroxisomal 3-oxoacyl-CoA thiolase from Saccharomyces cerevisiae.
Regulation of transcription of the gene coding for peroxisomal 3-oxoacyl-CoA thiolase of Saccharomyces cerevisiae.
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism.
The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology.
Identification of SAS4 and SAS5, two genes that regulate silencing in Saccharomyces cerevisiae.
SAS4 and SAS5 are locus-specific regulators of silencing in Saccharomyces cerevisiae.
Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex.
The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae.
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
Compartmentalization of the yeast meiotic nucleus revealed by analysis of ectopic recombination.
MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae.
Meiotic telomere protein Ndj1p is required for meiosis-specific telomere distribution, bouquet formation and efficient homologue pairing.
Ndj1, a telomere-associated protein, promotes meiotic recombination in budding yeast.
Ndj1p, a meiotic telomere protein required for normal chromosome synapsis and segregation in yeast.
Ndj1p-dependent epigenetic resetting of telomere size in yeast meiosis.
Restriction of ectopic recombination by interhomolog interactions during Saccharomyces cerevisiae meiosis.
Set1- and Clb5-deficiencies disclose the differential regulation of centromere and telomere dynamics in Saccharomyces cerevisiae meiosis.
Tam1, a telomere-associated meiotic protein, functions in chromosome synapsis and crossover interference.
Telomere-mediated chromosome pairing during meiosis in budding yeast.
Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces cerevisiae.
COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini.
Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method.
Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme.
The nuclear-coded subunits of yeast cytochrome c oxidase. II. The amino acid sequence of subunit VIII and a model for its disposition in the inner mitochondrial membrane.
A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating.
Genetic and epigenetic regulation of the FLO gene family generates cell-surface variation in yeast.
Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family.
A Hoogsteen base pair embedded in undistorted B-DNA.
A position effect in the control of transcription at yeast mating type loci.
A repressor (MAT alpha 2 Product) and its operator control expression of a set of cell type specific genes in yeast.
A sequence resembling a peroxisomal targeting sequence directs the interaction between the tetratricopeptide repeats of Ssn6 and the homeodomain of alpha 2.
Crystal structure of a MAT alpha 2 homeodomain-operator complex suggests a general model for homeodomain-DNA interactions.
Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA.
Crystal structure of the MATa1/MATalpha2 homeodomain heterodimer in complex with DNA containing an A-tract.
Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex.
Flexibility of the yeast alpha 2 repressor enables it to occupy the ends of its operator, leaving the center free.
Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins.
Homeo domain of the yeast repressor alpha 2 is a sequence-specific DNA-binding domain but is not sufficient for repression.
In vitro mutation analysis of the mating-type locus in yeast.
Molecular mechanisms of cell-type determination in budding yeast.
Physical analysis of mating-type loci in Saccharomyces cerevisiae.
Secondary structure of the homeo domain of yeast alpha 2 repressor determined by NMR spectroscopy.
Structural and thermodynamic characterization of the DNA binding properties of a triple alanine mutant of MATalpha2.
The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames.
The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae.
The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2.
The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes.
a/Alpha-specific repression by MAT alpha 2.
CASP, the alternatively spliced product of the gene encoding the CCAAT-displacement protein transcription factor, is a Golgi membrane protein related to giantin.
Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity.
Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport.
Live imaging of yeast Golgi cisternal maturation.
Ric1p and the Ypt6p GTPase function in a common pathway required for localization of trans-Golgi network membrane proteins.
Yeast Golgi SNARE interactions are promiscuous.
Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport.
A Plasmodium homolog of ER tubule-forming proteins is required for parasite virulence.
A conserved amphipathic helix is required for membrane tubule formation by Yop1p.
Function of a plant stress-induced gene, HVA22. Synthetic enhancement screen with its yeast homolog reveals its role in vesicular traffic.
The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum.
Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth.
Cystic fibrosis transmembrane conductance regulator degradation depends on the lectins Htm1p/EDEM and the Cdc48 protein complex in yeast.
Htm1p, a mannosidase-like protein, is involved in glycoprotein degradation in yeast.
Misfolded proteins are sorted by a sequential checkpoint mechanism of ER quality control.
Mnl1p, an alpha -mannosidase-like protein in yeast Saccharomyces cerevisiae, is required for endoplasmic reticulum-associated degradation of glycoproteins.
Single, context-specific glycans can target misfolded glycoproteins for ER-associated degradation.
A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1.
STP1, a gene involved in pre-tRNA processing, encodes a nuclear protein containing zinc finger motifs.
Characterization of a novel DNA damage-inducible gene of Saccharomyces cerevisiae, DIN7, which is a structural homolog of the RAD2 and RAD27 DNA repair genes.
Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation.
CLG1, a new cyclin-like gene of Saccharomyces cerevisiae.
A mutation in the yeast mitochondrial ribosomal protein Rml2p is associated with a defect in catalase gene expression.
A yeast strain defective in oleic acid utilization has a mutation in the RML2 gene.
Functional analysis of ribosomal protein L2 in yeast mitochondria.
Polyamine sensing by nascent ornithine decarboxylase antizyme stimulates decoding of its mRNA.
Polyamines regulate their synthesis by inducing expression and blocking degradation of ODC antizyme.
Yeast antizyme mediates degradation of yeast ornithine decarboxylase by yeast but not by mammalian proteasome: new insights on yeast antizyme.
The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae.
Molecular cloning and physical analysis of an 8.2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes.
Acm1 is a negative regulator of the CDH1-dependent anaphase-promoting complex/cyclosome in budding yeast.
Molecular characterization of transposable-element-associated mutations that lead to constitutive L-ornithine aminotransferase expression in Saccharomyces cerevisiae.
Characterization of Saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for G protein alpha subunit deacylation in vivo.
A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein.
Sterol binding assay using surface plasmon fluorescence spectroscopy.
NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes.
The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae.
The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA.
Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5.
Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22.
Import of mitochondrial carriers mediated by essential proteins of the intermembrane space.
Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria.
Tim9, a new component of the TIM22.54 translocase in mitochondria.
Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex.
Sorting of a multi-subunit ubiquitin ligase complex in the endolysosome system.
Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugates.
Centromere binding and a conserved role in chromosome stability for SUMO-dependent ubiquitin ligases.
Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase.
Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae.
Regulation of a spindle positioning factor at kinetochores by SUMO-targeted ubiquitin ligases.
SUMO-targeted ubiquitin ligase (STUbL) Slx5 regulates proteolysis of centromeric histone H3 variant Cse4 and prevents its mislocalization to euchromatin.
Slx5-Slx8 ubiquitin ligase targets active pools of the Yen1 nuclease to limit crossover formation.
Slx5/Slx8-dependent ubiquitin hotspots on chromatin contribute to stress tolerance.
Stimulation of in vitro sumoylation by Slx5-Slx8: evidence for a functional interaction with the SUMO pathway.
Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae.
The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks.
The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination.
The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation.
The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity.
A cycling protein complex required for selective autophagy.
ATG23, a novel gene required for maturation of proaminopeptidase I, but not for autophagy.
Atg23 is essential for the cytoplasm to vacuole targeting pathway and efficient autophagy but not pexophagy.
The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure.
SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.
Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.
Identification and analysis of the Saccharomyces cerevisiae SYR1 gene reveals that ergosterol is involved in the action of syringomycin.
Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway.
A member of a novel family of yeast 'Zn-finger' proteins mediates the transition from stationary phase to cell proliferation.
Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function.
The Gcs1 and Age2 ArfGAP proteins provide overlapping essential function for transport from the yeast trans-Golgi network.
The yeast ADP-ribosylation factor GAP, Gcs1p, is involved in maintenance of mitochondrial morphology.
DNA sequences in chromosomes II and VII code for pyruvate carboxylase isoenzymes in Saccharomyces cerevisiae: analysis of pyruvate carboxylase-deficient strains.
Polymorphism of the yeast pyruvate carboxylase 2 gene and protein: effects on protein biotinylation.
A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae.
Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions.
20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals.
Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes.
Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p.
Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly.
DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes.
Incorporation of Sed1p into the cell wall of Saccharomyces cerevisiae involves KRE6.
KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae.
Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae.
Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro.
Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria.
Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import.
SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import.
The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site.
The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.
Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1.
Cloning, primary structure and regulation of the ARO4 gene, encoding the tyrosine-inhibited 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae.
Evolution of feedback-inhibited beta/alpha barrel isoenzymes by gene duplication and a single mutation.
Mapping and sequencing of the dihydrofolate reductase gene (DFR1) of Saccharomyces cerevisiae.
Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1).
Nucleotide sequence of the dihydrofolate reductase gene of Saccharomyces cerevisiae.
PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p.
Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria.
TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway.
A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration.
ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae.
Expression in Escherichia coli of a recombinant adenosine kinase from Saccharomyces cerevisiae: purification, kinetics and substrate analyses.
Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes.
Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae.
Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface.
A highly conserved intraspecies homolog of the Saccharomyces cerevisiae elongation factor-3 encoded by the HEF3 gene.
Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae.
Purification procedure and N-terminal amino acid sequence of yeast malate dehydrogenase isoenzymes.
Raf60, a novel component of the Rpd3 histone deacetylase complex required for Rpd3 activity in Saccharomyces cerevisiae.
Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex.
ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes.
Molecular architecture and functional model of the complete yeast ESCRT-I heterotetramer.
Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant.
Structural and functional organization of the ESCRT-I trafficking complex.
Structural insight into the ESCRT-I/-II link and its role in MVB trafficking.
The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment.
Vps27 recruits ESCRT machinery to endosomes during MVB sorting.
Identification of the yeast mitochondrial transporter for oxaloacetate and sulfate.
Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones.
alpha-Isopropylmalate, a leucine biosynthesis intermediate in yeast, is transported by the mitochondrial oxalacetate carrier.
A molecular caliper mechanism for determining very long-chain fatty acid length.
Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles.
The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase.
Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae.
Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation.
Global protein expression profiling of budding yeast in response to DNA damage.
Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae.
Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae.
Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1.
Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants.
Function of the ste signal transduction pathway for mating pheromones sustains MAT alpha 1 transcription in Saccharomyces cerevisiae.
Identification of the mitochondrial GTP/GDP transporter in Saccharomyces cerevisiae.
Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast.
SHM1: a multicopy suppressor of a temperature-sensitive null mutation in the HMG1-like abf2 gene.
The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity.
CTD-dependent dismantling of the RNA polymerase II elongation complex by the pre-mRNA 3'-end processing factor, Pcf11.
Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae.
Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I.
Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination.
Key features of the interaction between Pcf11 CID and RNA polymerase II CTD.
PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I.
Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.
The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II.
Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.
Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures.
Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism.
Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae.
RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p.
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.
The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs.
U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.
X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.
Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance.
The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation.
Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein related to the mammalian membrane-associated progesterone receptor.
Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae.
The peroxisomal membrane protein Inp2p is the peroxisome-specific receptor for the myosin V motor Myo2p of Saccharomyces cerevisiae.
MRC1-dependent scaling of the budding yeast DNA replication timing program.
Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification.
The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant.
The TGL2 gene of Saccharomyces cerevisiae encodes an active acylglycerol lipase located in the mitochondria.
The mitochondrial intermembrane space-facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism.
A yeast strain biosensor to detect cell wall-perturbing agents.
Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway.
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex.
Differential export requirements for shuttling serine/arginine-type mRNA-binding proteins.
Discovery of arginine methylation, phosphorylation, and their co-occurrence in condensate-associated proteins in Saccharomyces cerevisiae.
Gbp2 interacts with THO/TREX through a novel type of RRM domain.
Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1.
Two yeast chromosomes are related by a fossil duplication of their centromeric regions.
A Rad26-Def1 complex coordinates repair and RNA pol II proteolysis in response to DNA damage.
An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae.
Architecture of the RNA polymerase II-Mediator core initiation complex.
Ask10p mediates the oxidative stress-induced destruction of the Saccharomyces cerevisiae C-type cyclin Ume3p/Srb11p.
C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats.
Construction and analysis of yeast RNA polymerase II CTD deletion and substitution mutations.
Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.
DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro.
Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.
Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.
Multiple mechanisms confining RNA polymerase II ubiquitylation to polymerases undergoing transcriptional arrest.
Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase.
Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress.
Regulation of the RNAPII Pool Is Integral to the DNA Damage Response.
Structure and function of the initially transcribing RNA polymerase II-TFIIB complex.
Temporal regulation of RNA polymerase II by Srb10 and Kin28 cyclin-dependent kinases.
The gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae.
Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant.
Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15.
Characteristics affecting expression and solubilization of yeast membrane proteins.
Global screening of genes essential for growth in high-pressure and cold environments: searching for basic adaptive strategies using a yeast deletion library.
Large-scale screening of yeast mutants for sensitivity to the IMP dehydrogenase inhibitor 6-azauracil.
Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein.
Evidence for a nuclear passage of nascent polypeptide-associated complex subunits in yeast.
Initial characterization of the nascent polypeptide-associated complex in yeast.
Nascent polypeptide-associated complex stimulates protein import into yeast mitochondria.
Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. Isolation by complementation of a mutant Escherichia coli suppressor tRNA defective in aminoacylation and sequence analysis.
The nascent polypeptide-associated complex (NAC) of yeast functions in the targeting process of ribosomes to the ER membrane.
The nascent polypeptide-associated complex (NAC) promotes interaction of ribosomes with the mitochondrial surface in vivo.
The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex.
The yeast EGD2 gene encodes a homologue of the alpha NAC subunit of the human nascent-polypeptide-associated complex.
The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria in vivo.
Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein.
Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses.
A single-stranded DNA binding protein required for mitochondrial DNA replication in S. cerevisiae is homologous to E. coli SSB.
Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein.
High-copy suppression of glucose transport defects by HXT4 and regulatory elements in the promoters of the HXT genes in Saccharomyces cerevisiae.
Low-affinity glucose carrier gene LGT1 of Saccharomyces cerevisiae, a homologue of the Kluyveromyces lactis RAG1 gene.
Xylose transport studies with xylose-utilizing Saccharomyces cerevisiae strains expressing heterologous and homologous permeases.
Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis.
A widespread transposable element masks expression of a yeast copper transport gene.
Antioxidant functions required for insusceptibility of Saccharomyces cerevisiae to tetracycline antibiotics.
C-terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: sub-femtomolar Cu(I) affinity of three proteins involved in copper trafficking.
Copper ion-sensing transcription factor Mac1p post-translationally controls the degradation of its target gene product Ctr1p.
Copper(II) protects yeast against the toxicity of cisplatin independently of the induction of metallothionein and the inhibition of platinum uptake.
Copper-dependent degradation of the Saccharomyces cerevisiae plasma membrane copper transporter Ctr1p in the apparent absence of endocytosis.
Copper-specific transcriptional repression of yeast genes encoding critical components in the copper transport pathway.
Dynamic regulation of copper uptake and detoxification genes in Saccharomyces cerevisiae.
Evidence for (Mac1p)2.DNA ternary complex formation in Mac1p-dependent transactivation at the CTR1 promoter.
Homeostatic regulation of copper uptake in yeast via direct binding of MAC1 protein to upstream regulatory sequences of FRE1 and CTR1.
Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays.
Mapping of the DNA binding domain of the copper-responsive transcription factor Mac1 from Saccharomyces cerevisiae.
Molecular characterization of a copper transport protein in S. cerevisiae: an unexpected role for copper in iron transport.
The Saccharomyces cerevisiae copper transport protein (Ctr1p). Biochemical characterization, regulation by copper, and physiologic role in copper uptake.
The copper transporter CTR1 regulates cisplatin uptake in Saccharomyces cerevisiae.
The yeast transcription factor Mac1 binds to DNA in a modular fashion.
Uptake of the anticancer drug cisplatin mediated by the copper transporter Ctr1 in yeast and mammals.
Dissecting the Genetic Basis of a Complex cis-Regulatory Adaptation.
New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system.
Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae.
Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway.
TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation.
Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae.
Regulation der Biosynthese der aromatischen Aminosaeuren in Saccharomyces cerevisiae.
Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames.
Studies on the structure, expression and function of the yeast regulatory gene PHO2.
The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual aminoacid composition.
The yeast regulatory gene PHO2 encodes a homeo box.
Inhibition of a spliceosome turnover pathway suppresses splicing defects.
Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0.
The alternate AP-1 adaptor subunit Apm2 interacts with the Mil1 regulatory protein and confers differential cargo sorting.
A third ADP/ATP translocator gene in yeast.
Structures of yeast mitochondrial ADP/ATP carriers support a domain-based alternating-access transport mechanism.
Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae.
A Cdc48p-associated factor modulates endoplasmic reticulum-associated degradation, cell stress, and ubiquitinated protein homeostasis.
Mechanism for recycling tRNAs on stalled ribosomes.
Structure and function of Vms1 and Arb1 in RQC and mitochondrial proteome homeostasis.
Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes.
Vms1p is a release factor for the ribosome-associated quality control complex.
A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.
Structural basis for COMPASS recognition of an H2B-ubiquitinated nucleosome.
Structural basis for histone H3K4me3 recognition by the N-terminal domain of the PHD finger protein Spp1.
Structure and conformational dynamics of a COMPASS histone H3K4 methyltransferase complex.
INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins.
A SNARE required for retrograde transport to the endoplasmic reticulum.
Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.
Isolation, DNA sequence and regulation of a new cell division cycle gene from the yeast Saccharomyces cerevisiae.
The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles.
Topological restriction of SNARE-dependent membrane fusion.
Use1p is a yeast SNARE protein required for retrograde traffic to the ER.
Yeast ribosomal proteins: XIII. Saccharomyces cerevisiae YL8A gene, interrupted with two introns, encodes a homolog of mammalian L7.
Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae.
SOK2 may regulate cyclic AMP-dependent protein kinase-stimulated growth and pseudohyphal development by repressing transcription.
Vid24p, a novel protein localized to the fructose-1, 6-bisphosphatase-containing vesicles, regulates targeting of fructose-1,6-bisphosphatase from the vesicles to the vacuole for degradation.
Glycerophosphocholine catabolism as a new route for choline formation for phosphatidylcholine synthesis by the Kennedy pathway.
Glycerophosphocholine-dependent growth requires Gde1p (YPL110c) and Git1p in Saccharomyces cerevisiae.
A large-scale study of Yap1p-dependent genes in normal aerobic and H2O2-stress conditions: the role of Yap1p in cell proliferation control in yeast.
A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation.
Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator.
Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor.
Genomic expression programs in the response of yeast cells to environmental changes.
H2O2 sensing through oxidation of the Yap1 transcription factor.
Identification and characterization of a Saccharomyces cerevisiae gene (PAR1) conferring resistance to iron chelators.
Molecular mechanism of oxidative stress perception by the Orp1 protein.
Multistep disulfide bond formation in Yap1 is required for sensing and transduction of H2O2 stress signal.
Regulation of the yeast Yap1p nuclear export signal is mediated by redox signal-induced reversible disulfide bond formation.
Structural basis for redox regulation of Yap1 transcription factor localization.
The PAR1 (YAP1/SNQ3) gene of Saccharomyces cerevisiae, a c-jun homologue, is involved in oxygen metabolism.
The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals.
The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H2O2 and diamide.
The redox domain of the Yap1p transcription factor contains two disulfide bonds.
The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability.
Transcriptional activation mediated by the yeast AP-1 protein is required for normal cadmium tolerance.
Two redox centers within Yap1 for H2O2 and thiol-reactive chemicals signaling.
Yap1 accumulates in the nucleus in response to carbon stress in Saccharomyces cerevisiae.
Yap1 activation by H2O2 or thiol-reactive chemicals elicits distinct adaptive gene responses.
Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast.
Ybp1 is required for the hydrogen peroxide-induced oxidation of the Yap1 transcription factor.
Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins.
The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation.
Identification of stop codon readthrough genes in Saccharomyces cerevisiae.
Genome-wide transcriptional analysis in S. cerevisiae by mini-array membrane hybridization.
3' poly(A) is dispensable for translation.
A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant.
An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation.
Chromosomal superkiller mutants of Saccharomyces cerevisiae.
Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system.
Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast.
Structure-based assembly of protein complexes in yeast.
Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN.
Synthetic lethality of sep1 (xrn1) ski2 and sep1 (xrn1) ski3 mutants of Saccharomyces cerevisiae is independent of killer virus and suggests a general role for these genes in translation control.
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.
Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs.
The functioning of the yeast Golgi apparatus requires an ER protein encoded by ANP1, a member of a new family of genes affecting the secretory pathway.
A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis.
Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis.
Changes in the localization of the Saccharomyces cerevisiae anaphase-promoting complex upon microtubule depolymerization and spindle checkpoint activation.
Cloning and nucleotide sequence of the CDC23 gene of Saccharomyces cerevisiae.
Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex.
The destruction box of the cyclin Clb2 binds the anaphase-promoting complex/cyclosome subunit Cdc23.
p62cdc23 of Saccharomyces cerevisiae: a nuclear tetratricopeptide repeat protein with two mutable domains.
Identification of mitogen-activated protein kinase signaling pathways that confer resistance to endoplasmic reticulum stress in Saccharomyces cerevisiae.
Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry.
Interactions within the yeast Sm core complex: from proteins to amino acids.
Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain.
Primary structure of the uracil transport protein of Saccharomyces cerevisiae.
Characterization of the KIN2 gene product in Saccharomyces cerevisiae and comparison between the kinase activities of p145KIN1 and p145KIN2.
KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane.
The product of the KIN1 locus in Saccharomyces cerevisiae is a serine/threonine-specific protein kinase.
The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery.
Two yeast genes that encode unusual protein kinases.
Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns.
Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p.
Defining protein interactions with yeast actin in vivo.
In vivo analysis of the domains of yeast Rvs167p suggests Rvs167p function is mediated through multiple protein interactions.
Interaction of yeast Rvs167 and Pho85 cyclin-dependent kinase complexes may link the cell cycle to the actin cytoskeleton.
Regulation of the yeast amphiphysin homologue Rvs167p by phosphorylation.
Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
Modulation of Ras and a-factor function by carboxyl-terminal proteolysis.
Nucleotide sequence of two rasH related-genes isolated from the yeast Saccharomyces cerevisiae.
Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.
RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins.
The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins.
The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms.
Atg1 kinase organizes autophagosome formation by phosphorylating Atg9.
Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate.
Atg21 is required for effective recruitment of Atg8 to the preautophagosomal structure during the Cvt pathway.
Autophagosome formation can be achieved in the absence of Atg18 by expressing engineered PAS-targeted Atg2.
Autophagy and the cytoplasm to vacuole targeting pathway both require Aut10p.
Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris.
Dissecting the localization and function of Atg18, Atg21 and Ygr223c.
Early steps in autophagy depend on direct phosphorylation of Atg9 by the Atg1 kinase.
Functional analysis of the Saccharomyces cerevisiae YFR021w/YGR223c/YPL100w ORF family suggests relations to mitochondrial/peroxisomal functions and amino acid signalling pathways.
GFP-Atg8 protease protection as a tool to monitor autophagosome biogenesis.
KCS1 deletion in Saccharomyces cerevisiae leads to a defect in translocation of autophagic proteins and reduces autophagosome formation.
Phosphoinositide [PI(3,5)P2] lipid-dependent regulation of the general transcriptional regulator Tup1.
Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy.
Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy.
Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a beta-propeller protein family.
Svp1p defines a family of phosphatidylinositol 3,5-bisphosphate effectors.
The Atg18-Atg2 complex is recruited to autophagic membranes via phosphatidylinositol 3-phosphate and exerts an essential function.
The crystal structure of Atg18 reveals a new binding site for Atg2 in Saccharomyces cerevisiae.
The relevance of the phosphatidylinositolphosphat-binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy.
Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy.
VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.
Yeast vacuoles fragment in an asymmetrical two-phase process with distinct protein requirements.
Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae.
The mechanism of tail-anchored protein insertion into the ER membrane.
CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c.
Cyc2p, a membrane-bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes.
The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae.
Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases.
The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.
Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae.
A histone fold TAF octamer within the yeast TFIID transcriptional coactivator.
The yeast SUA7 gene encodes a homolog of human transcription factor TFIIB and is required for normal start site selection in vivo.
Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p.
Feedback control of mitosis in budding yeast.
The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase.
TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae.
Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing.
Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo.
Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins.
Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae.
Molecular evidence for a positive role of Spt4 in transcription elongation.
SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat.
The Ras/PKA signaling pathway may control RNA polymerase II elongation via the Spt4p/Spt5p complex in Saccharomyces cerevisiae.
A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein.
The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae.
The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump.
Dissection of the bifunctional ARGRII protein involved in the regulation of arginine anabolic and catabolic pathways.
Nucleotide sequence of the ARGRII regulatory gene and amino acid sequence homologies between ARGRII PPRI and GAL4 regulatory proteins.
Cloning and characterization of a yeast cytochrome b5-encoding gene which suppresses ketoconazole hypersensitivity in a NADPH-P-450 reductase-deficient strain.
Characterization of a temperature-sensitive yeast vacuolar ATPase mutant with defects in actin distribution and bud morphology.
Crystal structure of yeast V1-ATPase in the autoinhibited state.
Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H(+)-ATPase.
Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase.
Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase.
Mutational analysis of the stator subunit E of the yeast V-ATPase.
Resolution of subunit interactions and cytoplasmic subcomplexes of the yeast vacuolar proton-translocating ATPase.
Stoichiometry of the peripheral stalk subunits E and G of yeast V1-ATPase determined by mass spectrometry.
The 31-kDa polypeptide is an essential subunit of the vacuolar ATPase in Saccharomyces cerevisiae.
A member of the YER057c/yjgf/Uk114 family links isoleucine biosynthesis and intact mitochondria maintenance in Saccharomyces cerevisiae.
Mmf1p, a novel yeast mitochondrial protein conserved throughout evolution and involved in maintenance of the mitochondrial genome.
X-ray structure of Saccharomyces cerevisiae homologous mitochondrial matrix factor 1 (Hmf1).
A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome.
Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum.
Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus.
A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.
Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits.
Molecular analysis of the PHO81 gene of Saccharomyces cerevisiae.
Nucleotide sequence of the PHO81 gene involved in the regulation of the repressible acid phosphatase gene in Saccharomyces cerevisiae.
Phosphate-regulated inactivation of the kinase PHO80-PHO85 by the CDK inhibitor PHO81.
Promoter analysis of the PHO81 gene encoding a 134 kDa protein bearing ankyrin repeats in the phosphatase regulon of Saccharomyces cerevisiae.
Regulation by phosphorylation of Pho81p, a cyclin-dependent kinase inhibitor in Saccharomyces cerevisiae.
The yeast Pho80-Pho85 cyclin-CDK complex has multiple substrates.
Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes.
The 26S proteasome of the yeast Saccharomyces cerevisiae.
Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.
The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity.
Architecture of the Mediator head module.
Head module control of mediator interactions.
Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae.
Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.
Mediator-dependent recruitment of TFIIH modules in preinitiation complex.
Cloning and analysis of the nuclear gene for YmL33, a protein of the large subunit of the mitochondrial ribosome in Saccharomyces cerevisiae.
The novel nuclear gene DSS-1 of Saccharomyces cerevisiae is necessary for mitochondrial biogenesis.
The yeast nuclear gene DSS1, which codes for a putative RNase II, is necessary for the function of the mitochondrial degradosome in processing and turnover of RNA.
Different mating-type-regulated genes affect the DNA repair defects of Saccharomyces RAD51, RAD52 and RAD55 mutants.
A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain.
Autophagic clearance of PolyQ proteins mediated by ubiquitin-Atg8 adaptors of the conserved CUET protein family.
Targeted proteomic study of the cyclin-Cdk module.
Identification of protein N-terminal methyltransferases in yeast and humans.
The gene for yeast ribosomal protein S31 contains an intron in the leader sequence.
Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes.
Crystal structure of the YGR205w protein from Saccharomyces cerevisiae: close structural resemblance to E. coli pantothenate kinase.
Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase.
A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with F0F1 ATP synthase and confers calcium-sensitive growth.
Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity.
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.
Homing in on an endosymbiotic endonuclease.
Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae.
Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.
Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing.
New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae.
Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking.
Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase.
Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision.
Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides.
Structural insights into the protein splicing mechanism of PI-SceI.
Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp.
Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide.
A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae.
Activation of yeast protein kinase C by Rho1 GTPase.
Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7.
Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae.
Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae.
Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae.
Complementing yeast rho1 mutation groups with distinct functional defects.
Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast.
Gbetagamma recruits Rho1 to the site of polarized growth during mating in budding yeast.
Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae.
Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase.
Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast.
Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane.
ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae.
Rho1 directs formin-mediated actin ring assembly during budding yeast cytokinesis.
Rho1p, a yeast protein at the interface between cell polarization and morphogenesis.
Rom1p and Rom2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae.
Spatial regulation of the exocyst complex by Rho1 GTPase.
The RHO1-GAPs SAC7, BEM2 and BAG7 control distinct RHO1 functions in Saccharomyces cerevisiae.
The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast.
The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2.
The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane.
Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis.
Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast.
Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.
The nucleotide sequence of the HIS4 region of yeast.
Transposable element sequences involved in the enhancement of yeast gene expression.
Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene.
Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate.
Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments.
Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity.
Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.
Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.
Okazaki fragment maturation in yeast. I. Distribution of functions between FEN1 and DNA2.
Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product.
Reconstituted Okazaki fragment processing indicates two pathways of primer removal.
Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases.
The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1.
Two modes of FEN1 binding to PCNA regulated by DNA.
AP1-mediated multidrug resistance in Saccharomyces cerevisiae requires FLR1 encoding a transporter of the major facilitator superfamily.
A Saccharomyces cerevisiae homolog of the human adrenoleukodystrophy transporter is a heterodimer of two half ATP-binding cassette transporters.
The ABC transporter proteins Pat1 and Pat2 are required for import of long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae.
The human peroxisomal ABC half transporter ALDP functions as a homodimer and accepts acyl-CoA esters.
The yeast YKL741 gene situated on the left arm of chromosome XI codes for a homologue of the human ALD protein.
Saccharomyces cerevisiae multidrug resistance transporter Qdr2 is implicated in potassium uptake, providing a physiological advantage to quinidine-stressed cells.
Saccharomyces cerevisiae multidrug transporter Qdr2p (Yil121wp): localization and function as a quinidine resistance determinant.
Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3.
The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase.
Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrome oxidase.
The primary structure of the mitochondrial genome of Saccharomyces cerevisiae -- a review.
Characterization of the putative maltose transporters encoded by YDL247w and YJR160c.
Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.
A homolog of lariat-debranching enzyme modulates turnover of branched RNA.
A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways.
A new yeast endosomal SNARE related to mammalian syntaxin 8.
A t-SNARE of the endocytic pathway must be activated for fusion.
Characterization of a novel yeast SNARE protein implicated in Golgi retrograde traffic.
The Saccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole.
The specificity of SNARE-dependent fusion is encoded in the SNARE motif.
The yeast v-SNARE Vti1p mediates two vesicle transport pathways through interactions with the t-SNAREs Sed5p and Pep12p.
Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion.
Two syntaxin homologues in the TGN/endosomal system of yeast.
An inhibitor of yeast cyclin-dependent protein kinase plays an important role in ensuring the genomic integrity of daughter cells.
Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1.
P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae.
Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation.
The Cdk-activating kinase (CAK) from budding yeast.
Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family.
Rad33, a new factor involved in nucleotide excision repair in Saccharomyces cerevisiae.
Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis.
Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p.
Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae.
Pmp27 promotes peroxisomal proliferation.
The peroxin Pex34p functions with the Pex11 family of peroxisomal divisional proteins to regulate the peroxisome population in yeast.
A yeast mitochondrial outer membrane protein essential for protein import and cell viability.
Biogenesis of Tom40, core component of the TOM complex of mitochondria.
Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex.
Cytochrome c methyltransferase, Ctm1p, of yeast.
Determinants of cytochrome c pro-apoptotic activity. The role of lysine 72 trimethylation.
Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria.
High-resolution refinement of yeast iso-1-cytochrome c and comparisons with other eukaryotic cytochromes c.
Isoforms of yeast cytochrome c oxidase subunit V affect the binuclear reaction center and alter the kinetics of interaction with the isoforms of yeast cytochrome c.
Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae.
Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X.
Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
Sequence of the yeast iso-1-cytochrome c mRNA.
Solution structure of oxidized Saccharomyces cerevisiae iso-1-cytochrome c.
Studies on cytochrome c. 8. Synthesis of the protected hexadecapeptide (sequence 93-108) of Baker's yeast iso-1-cytochrome c.
The complete amino acid sequence in baker's yeast cytochrome c.
Three-dimensional solution structure of Saccharomyces cerevisiae reduced iso-1-cytochrome c.
Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c gene. Multiple, independent T-A-T-A sequences.
Yeast iso-1-cytochrome c. A 2.8-A resolution three-dimensional structure determination.
An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system.
Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.
Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry.
Transport mechanism of P4 ATPase phosphatidylcholine flippases.
A protein-protein interaction map of yeast RNA polymerase III.
A subcomplex of RNA polymerase III subunits involved in transcription termination and reinitiation.
RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest.
The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation.
Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B.
Biochemical evidence for the orientation of cytochrome b in the yeast mitochondrial membrane in the eight-helix model.
Electron-transfer restoration by vitamin K3 in a complex III-deficient mutant of S. cerevisiae and sequence of the corresponding cytochrome b mutation.
Limitations to in vivo import of hydrophobic proteins into yeast mitochondria. The case of a cytoplasmically synthesized apocytochrome b.
Revision of the nucleotide sequence at the last intron of the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae.
Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.
Splice points of the third intron in the yeast mitochondrial cytochrome b gene.
The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.
The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145.
Global analysis of protein phosphorylation in yeast.
Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast.
Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family.
SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition.
Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae.
A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication.
Interaction between the N-terminal and middle regions is essential for the in vivo function of HSP90 molecular chaperone.
Role of HSP90 in salt stress tolerance via stabilization and regulation of calcineurin.
Sgt1 associates with Hsp90: an initial step of assembly of the core kinetochore complex.
Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index.
hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.
Response of genes associated with mitochondrial function to mild heat stress in yeast Saccharomyces cerevisiae.
Analysis of a 26 kb region on the left arm of yeast chromosome XV.
Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae.
Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli.
PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3.
In silico identification of a new group of specific bacterial and fungal nitroreductases-like proteins.
Cloning and expression of a yeast protein tyrosine phosphatase.
The CLK family kinases, CLK1 and CLK2, phosphorylate and activate the tyrosine phosphatase, PTP-1B.
A Saccharomyces cerevisiae cyclophilin resident in the endoplasmic reticulum.
All cyclophilins and FK506 binding proteins are, individually and collectively, dispensable for viability in Saccharomyces cerevisiae.
A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast.
Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function.
eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange.
Isolation of the gene encoding the S. cerevisiae heat shock transcription factor.
Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation.
Yeast heat shock transcription factor contains a flexible linker between the DNA-binding and trimerization domains. Implications for DNA binding by trimeric proteins.
Ammonia pulses and metabolic oscillations guide yeast colony development.
An essential and specific subunit of RNA polymerase III (C) is encoded by gene RPC34 in Saccharomyces cerevisiae.
Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches.
Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica.
Complete amino acid sequence of flavocytochrome b2 from baker's yeast.
Complete amino acid sequence of the heme-binding core in bakers' yeast cytochrome b2 (L-(+)-lactate dehydrogenase).
Crystallographic study of the recombinant flavin-binding domain of Baker's yeast flavocytochrome b(2): comparison with the intact wild-type enzyme.
Cytochrome b2 from bakers' yeast (L-lactate dehydrogenase). A double-headed enzyme.
Mechanistic and structural studies of H373Q flavocytochrome b2: effects of mutating the active site base.
Molecular structure of flavocytochrome b2 at 2.4-A resolution.
More similarity between bakers'yeast L-(+)-lactate dehydrogenase and liver microsomal sytochrome B5.
Primary structure of flavocytochrome b2 from baker's yeast. Purification by reverse-phase high-pressure liquid chromatography and sequencing of fragment alpha cyanogen bromide peptides.
Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2).
Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast.
Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames.
Gene for yeast glutamine tRNA synthetase encodes a large amino-terminal extension and provides a strong confirmation of the signature sequence for a group of the aminoacyl-tRNA synthetases.
Genome-wide identification of fungal GPI proteins.
The 3.5-A cryoEM structure of nanodisc-reconstituted yeast vacuolar ATPase V0 proton channel.
Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum.
Homologues of 26S proteasome subunits are regulators of transcription and translation.
Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits.
Asc1, Hel2, and Slh1 couple translation arrest to nascent chain degradation.
Decoding of the ubiquitin code for clearance of colliding ribosomes by the RQT complex.
RQT complex dissociates ribosomes collided on endogenous RQC substrate SDD1.
Ubiquitination of stalled ribosome triggers ribosome-associated quality control.
Apg10p, a novel protein-conjugating enzyme essential for autophagy in yeast.
Apg7p/Cvt2p: a novel protein-activating enzyme essential for autophagy.
Endoplasmic reticulum stress triggers autophagy.
New insights into autophagy using a multiple knockout strain.
The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation.
The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.
Gene-enzyme relations in histidine biosynthesis in yeast.
Structure of the yeast HIS5 gene responsive to general control of amino acid biosynthesis.
Regulation of the pyrimidine salvage pathway by the FUR1 gene product of Saccharomyces cerevisiae.
The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles.
Amino acid sequence similarity between GCN3 and GCD2, positive and negative translational regulators of GCN4: evidence for antagonism by competition.
A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae.
A novel zinc finger is required for Mcm10 homocomplex assembly.
Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae.
Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast.
Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae.
Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins.
Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae.
Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.
Two bipartite NLSs mediate constitutive nuclear localization of Mcm10.
Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions.
Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation.
Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae.
Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor.
Sequence of the HMR region on chromosome III of Saccharomyces cerevisiae.
A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease.
Chromosome-wide Rad51 spreading and SUMO-H2A.Z-dependent chromosome fixation in response to a persistent DNA double-strand break.
Functional analysis of the yeast LINC complex using fluctuation spectroscopy and super-resolution imaging.
Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery.
Meiosis-specific cohesin component, Rec8, promotes the localization of Mps3 SUN domain protein on the nuclear envelope.
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.
Mps3 SUN domain is important for chromosome motion and juxtaposition of homologous chromosomes during meiosis.
Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p.
Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion.
Opening the DNA repair toolbox: localization of DNA double strand breaks to the nuclear periphery.
Phosphorylation of luminal region of the SUN-domain protein Mps3 promotes nuclear envelope localization during meiosis.
Sister-chromatid telomere cohesion is nonredundant and resists both spindle forces and telomere motility.
Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z.
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope.
The nuclear envelope and spindle pole body-associated Mps3 protein bind telomere regulators and function in telomere clustering.
The spindle pole body assembly component Mps3p/Nep98p functions in sister chromatid cohesion.
Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombination.
Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli.
Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes.
Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3).
Purification of a ubiquitin protein peptidase from yeast with efficient in vitro assays.
Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p.
Structural basis for the specificity of ubiquitin C-terminal hydrolases.
Arginine methylation promotes translation repression activity of eIF4G-binding protein, Scd6.
Scd6 targets eIF4G to repress translation: RGG motif proteins as a class of eIF4G-binding proteins.
Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex.
Role of Vma21p in assembly and transport of the yeast vacuolar ATPase.
Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex.
Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase.
Philos. Trans. R. Soc. Lond., B, Biol. Sci.
Development of a mutagenesis, expression and purification system for yeast phosphoglycerate mutase. Investigation of the role of active-site His181.
Dissociation of the tetrameric phosphoglycerate mutase from yeast by a mutation in the subunit contact region.
Polyanionic inhibitors of phosphoglycerate mutase: combined structural and biochemical analysis.
Sequence and localization of the gene encoding yeast phosphoglycerate mutase.
Sequence of the gene encoding phosphoglycerate mutase from Saccharomyces cerevisiae.
Structure and activity of phosphoglycerate mutase.
Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 A.
Sulphate ions observed in the 2.12 A structure of a new crystal form of S. cerevisiae phosphoglycerate mutase provide insights into understanding the catalytic mechanism.
The 2.3 A X-ray crystal structure of S. cerevisiae phosphoglycerate mutase.
The amino acid sequence of yeast phosphoglycerate mutase.
Transcriptional control of yeast phosphoglycerate mutase-encoding gene.
Acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial intermembrane space.
Global analysis of the mitochondrial N-proteome identifies a processing peptidase critical for protein stability.
Mutants of Saccharomyces cerevisiae with defects in acetate metabolism: isolation and characterization of Acn- mutants.
The LYR factors SDHAF1 and SDHAF3 mediate maturation of the iron-sulfur subunit of succinate dehydrogenase.
Yeast mutants of glucose metabolism with defects in the coordinate regulation of carbon assimilation.
An inducible chaperone adapts proteasome assembly to stress.
Ty4, a new retrotransposon from Saccharomyces cerevisiae, flanked by tau-elements.
A latent intron-encoded maturase is also an endonuclease needed for intron mobility.
Connections between RNA splicing and DNA intron mobility in yeast mitochondria: RNA maturase and DNA endonuclease switching experiments.
In vivo and in vitro analyses of an intron-encoded DNA endonuclease from yeast mitochondria. Recognition site by site-directed mutagenesis.
Purification of a site-specific endonuclease, I-Sce II, encoded by intron 4 alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae.
Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene.
Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria.
Sequence, map position and genome organization of the RPL17B gene, encoding ribosomal protein L17b in Saccharomyces cerevisiae.
Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption.
Identification of the upstream activation sequences responsible for the expression and regulation of the PEM1 and PEM2 genes encoding the enzymes of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae.
Phosphatidylethanolamine methyltransferase and phospholipid methyltransferase activities from Saccharomyces cerevisiae. Enzymological and kinetic properties.
Quantitative profiling of PE, MMPE, DMPE, and PC lipid species by multiple precursor ion scanning: a tool for monitoring PE metabolism.
Regulation of phosphatidylethanolamine methyltransferase and phospholipid methyltransferase by phospholipid precursors in Saccharomyces cerevisiae.
Regulation of phosphatidylethanolamine methyltransferase level by myo-inositol in Saccharomyces cerevisiae.
Regulation of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae.
Saccharomyces cerevisiae cho2 mutants are deficient in phospholipid methylation and cross-pathway regulation of inositol synthesis.
The yeast phospholipid N-methyltransferases catalyzing the synthesis of phosphatidylcholine preferentially convert di-C16:1 substrates both in vivo and in vitro.
Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes.
Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae.
Early expression of yeast genes affected by chemical stress.
Vhr1p, a new transcription factor from budding yeast, regulates biotin-dependent expression of VHT1 and BIO5.
Characterization of the Wtm proteins, a novel family of Saccharomyces cerevisiae transcriptional modulators with roles in meiotic regulation and silencing.
Ume1p represses meiotic gene transcription in Saccharomyces cerevisiae through interaction with the histone deacetylase Rpd3p.
Saccharomyces cerevisiae forms D-2-hydroxyglutarate and couples its degradation to D-lactate formation via a cytosolic transhydrogenase.
Analysis of quality control substrates in distinct cellular compartments reveals a unique role for Rpn4p in tolerating misfolded membrane proteins.
Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded.
Minimal nuclear pore complexes define FG repeat domains essential for transport.
Proteomic analysis of nucleoporin interacting proteins.
The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex.
MNN6, a member of the KRE2/MNT1 family, is the gene for mannosylphosphate transfer in Saccharomyces cerevisiae.
A role in vacuolar arginine transport for yeast Btn1p and for human CLN3, the protein defective in Batten disease.
Action of BTN1, the yeast orthologue of the gene mutated in Batten disease.
BTN1, a yeast gene corresponding to the human gene responsible for Batten's disease, is not essential for viability, mitochondrial function, or degradation of mitochondrial ATP synthase.
The subcellular location of the yeast Saccharomyces homologue of the protein defective in the juvenile form of Batten disease.
A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme.
A novel mitochondrial DEAD box protein (Mrh4) required for maintenance of mtDNA in Saccharomyces cerevisiae.
Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen.
Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast.
Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast.
KH domain-containing proteins of yeast: absence of a fragile X gene homologue.
Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p.
Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast.
The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast.
The binding activity of yeast RNAs to yeast Hek2p and mammalian hnRNP K proteins, determined using the three-hybrid system.
Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length.
Characterization of a second nuclear gene, AEP1, required for expression of the mitochondrial OLI1 gene in Saccharomyces cerevisiae.
Nuclear control of the messenger RNA expression for mitochondrial ATPase subunit 9 in a new yeast mutant.
Repression by the yeast meiotic inhibitor RME1.
Rme1, a negative regulator of meiosis, is also a positive activator of G1 cyclin gene expression.
The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-alpha 2.
Isolation of the ALG6 locus of Saccharomyces cerevisiae required for glucosylation in the N-linked glycosylation pathway.
Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.
Genetic and physical mapping of the WBP1 locus close to CENV.
A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.
Components of a Fanconi-like pathway control Pso2-independent DNA interstrand crosslink repair in yeast.
Genome-wide requirements for resistance to functionally distinct DNA-damaging agents.
MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage.
Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates.
Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase.
The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing.
Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from homologous recombination, but not from postreplicative repair.
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae.
Conservative duplication of spindle poles during meiosis in Saccharomyces cerevisiae.
SPO21 is required for meiosis-specific modification of the spindle pole body in yeast.
Two novel deduced serine/threonine protein kinases from Saccharomyces cerevisiae.
Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins.
Maintenance of mating cell integrity requires the adhesin Fig2p.
Pheromone-regulated genes required for yeast mating differentiation.
Role of Fig2p in agglutination in Saccharomyces cerevisiae.
The complete sequence of a 6146 bp fragment of Saccharomyces cerevisiae chromosome III contains two new open reading frames.
Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription.
Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae.
HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription.
Yeast HOS3 forms a novel trichostatin A-insensitive homodimer with intrinsic histone deacetylase activity.
Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme.
dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae.
A second transport ATPase gene in Saccharomyces cerevisiae.
SEM1, a homologue of the split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast.
Sem1p is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae.
Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins.
Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast.
Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein.
The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a.
Yeast virus propagation depends critically on free 60S ribosomal subunit concentration.
A novel role for yeast casein kinases in glucose sensing and signaling.
Dominant and recessive suppressors that restore glucose transport in a yeast snf3 mutant.
Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae.
Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I.
Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.
A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae.
Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene.
Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p.
The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast.
Loss of SOD1 and LYS7 sensitizes Saccharomyces cerevisiae to hydroxyurea and DNA damage agents and downregulates MEC1 pathway effectors.
NORF5/HUG1 is a component of the MEC1-mediated checkpoint response to DNA damage and replication arrest in Saccharomyces cerevisiae.
A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae.
A genomics-based screen for yeast mutants with an altered recombination/end-joining repair ratio.
Mutations of the Yku80 C terminus and Xrs2 FHA domain specifically block yeast nonhomologous end joining.
NEJ1 controls non-homologous end joining in Saccharomyces cerevisiae.
NEJ1 prevents NHEJ-dependent telomere fusions in yeast without telomerase.
NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway.
Nej1p, a cell type-specific regulator of nonhomologous end joining in yeast.
Biosynthesis of the thiamin-thiazole in eukaryotes: identification of a thiazole tautomer intermediate.
Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.
Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance.
Heat stress promotes mitochondrial instability and oxidative responses in yeast deficient in thiazole biosynthesis.
MOL1, a Saccharomyces cerevisiae gene that is highly expressed in early stationary phase during growth on molasses.
Neurospora crassa CyPBP37: a cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B1.
Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae.
Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae.
Thiamin biosynthesis in eukaryotes: characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis.