565042:BLLJ RS09570
gene id: 565042:BLLJ_RS09570
uniprot id: A0A3R0A696
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 43 family.
messages: Cell membrane
messages: Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides (PubMed:30564851). Can use radish root AGP, larch AG and arabinan. Shows weaker activity with gum arabic and arabinoxylan (PubMed:30564851).

565042:BLLJ RS08355
gene id: 565042:BLLJ_RS08355
uniprot id: E8MF10
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.
messages: Homodimer.
messages: Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. Can also epimerize UDP-GalNAc to UDP-GlcNAc. Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria.

565042:BLLJ RS07765
gene id: 565042:BLLJ_RS07765
uniprot id: P0DW94
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Chaperone required for active expression of the lacto-N-biosidase LnbX.
messages: Homodimer.
messages: Secreted

565042:BLLJ RS08365
gene id: 565042:BLLJ_RS08365
uniprot id: E8MF12
names: Function
names: Similarity
messages: Belongs to the protein kinase superfamily.
messages: Phosphorylates both N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) at similar rates. Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria. Also accepts GTP and ITP as phosphate donors. In vitro, can phosphorylate several GlcNAc and GalNAc derivatives.

565042:BLLJ RS01095
gene id: 565042:BLLJ_RS01095
uniprot id: E8MGH9
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 121 family.
messages: Beta-L-arabinobiosidase that removes L-arabinofuranose-beta-1,2-L-arabinofuranose disaccharide from various substrates such as carrot extensin and potato lectin. Also acts on L-arabinofuranose (Ara)-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp) but not on Ara-beta-1,3-Ara-beta-1,2-Ara-beta-1,2-Ara-beta--Hyp (Ara(4)-Hyp) or Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), suggesting a specificity for unmodified Ara(3)-Hyp substrate. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.
messages: Membrane
messages: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

565042:BLLJ RS06645
gene id: 565042:BLLJ_RS06645
uniprot id: E8ME30
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allosterically activated by fructose 1,6-bisphosphate (FBP).
messages: Belongs to the LDH/MDH superfamily. LDH family.
messages: Catalyzes the conversion of lactate to pyruvate.
messages: Cytoplasm
messages: Homotetramer.

565042:BLLJ RS08360
gene id: 565042:BLLJ_RS08360
uniprot id: E8MF11
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the galactose-1-phosphate uridylyltransferase type 2 family.
messages: Cytoplasm
messages: Transfers the UMP unit from UDP-glucose (UDP-Glc) to Gal1P. Can also transfer the UMP unit to GlcNAc1P and GalNAc1P. Involved in the general galactose metabolism, and also involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria.

565042:BLLJ RS08370
gene id: 565042:BLLJ_RS08370
uniprot id: E8MF13
names: Function
names: Similarity
names: Subunit
messages: Belongs to the glycoside hydrolase 112 family.
messages: Homodimer.
messages: Reversibly phosphorolyzes lacto-N-biose to Gal1-P and N-acetylglucosamine (GlcNAc) and galacto-N-biose to Gal1-P and N-acetylgalactosamine (GalNAc). Involved in the lacto-N-biose I/galacto-N-biose (LNB/GNB) degradation pathway, which is important for host intestinal colonization by bifidobacteria.

565042:BLLJ RS01090
gene id: 565042:BLLJ_RS01090
uniprot id: E8MGH8
names: Activity Regulation
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the glycosyl hydrolase 127 family.
messages: Beta-L-arabinofuranosidase that removes the beta-L-arabinofuranose residue from the non-reducing end of various substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara-beta-1,2-Ara-beta-Hyp (Ara(2)-Hyp), Ara-beta-1,2-Ara-beta-1,2-Ara-beta-Hyp (Ara(3)-Hyp), and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, shows transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.
messages: Homodimer in solution.
messages: Strongly inhibited in the presence of thiol modifiers, suggesting a crucial role for cysteine residues in catalysis. Slightly inhibited by EDTA.
messages: The original article describing the function has been retracted because the results of E338A and E366A mutants were reversed. The authors later submitted a corrected manuscript.
messages: Zn(2+) ion is involved in the catalytic reaction through maintaining the proper configuration of active site.

565042:BLLJ RS05690
gene id: 565042:BLLJ_RS05690
uniprot id: E8MJ15
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the LDH/MDH superfamily. LDH family.
messages: Catalyzes the conversion of lactate to pyruvate.
messages: Cytoplasm
messages: Homotetramer.

565042:BLLJ RS09505
gene id: 565042:BLLJ_RS09505
uniprot id: A0A401ETL2
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 30 family.
messages: Cell membrane
messages: Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851). Specifically releases the non-reducing terminal beta-1,6-galactobiose (beta-1,6-Gal2) from both dearabinosylated larch AG and polymeric beta-1,6-galactan chains by an exo-mode of action (PubMed:30564851). Shows lower activity with larch AG, and very weak activity with dearabinosylated gum arabic, gum arabic and potato galactan (PubMed:30564851). Can probably release beta-1,6-Gal2 from the internal side chains of type II AG (PubMed:30564851).

565042:BLLJ RS10055
gene id: 565042:BLLJ_RS10055
uniprot id: P0DW93
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 136 (GH136) family.
messages: Binds 1 Mg(2+) ion per subunit. Magnesium ions are required for efficient protein folding.
messages: Binds 3 Ca(2+) ions per subunit. Calcium ions are required for efficient protein folding.
messages: Breast-fed infants have gut microbiota that are substantially more rich in bifidobacteria compared with the mix-fed infants. Notably, B.longum was found to significantly dominate among members of the genus Bifidobacterium in the breast-fed infants.
messages: Cell membrane
messages: Cells lacking this gene lose the ability to grow on LNT but continue to grow well on lactose and LNB.
messages: HMOs function as prebiotics, promoting the growth of bifidobacteria in the gastrointestinal tracts of breast-fed infants, which in turn promotes optimal health.
messages: Homodimer.
messages: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
messages: Present in the infant gut, this enzyme is involved in the assimilation of type-1 human milk oligosaccharides (HMOs) (PubMed:23843461, PubMed:28392148). It hydrolyzes via a retaining mechanism the beta-D-GlcNAc-(1->3)-beta-D-Gal linkage in lacto-N-tetraose (LNT or beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc), an abundant HMO unique to human breast milk, releasing lacto-N-biose (LNB or beta-D-Gal-(1->3)-D-GlcNAc) and lactose. With a much lower efficiency, is also able to cleave the same linkage in lacto-N-fucopentaose I (alpha-Fuc(1->2)-beta-D-Gal-(1->3)-beta-D-GlcNAc(1->3)-beta-D-Gal-(1->4)-D-Glc), and sialyllacto-N-tetraose a (alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc) (PubMed:23843461). Is a key enzymatic factor for growth and proliferation of B.longum in the gut ecosystem of breast-fed infants (PubMed:28392148).
messages: Requires the chaperone LnbY for its proper folding and active expression (PubMed:23843461). Is potently and competitively inhibited by LNB-PUGNAc and LNB-NHAcDNJ in vitro (PubMed:28392148).
messages: The region of amino acids 31-625 was identified as the minimum catalytic unit exhibiting full activity.