Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.
Belongs to the site-specific recombinase resolvase family.
Belongs to the flavin monoamine oxidase family.
Consists of two domains, an FAD-binding domain and a substrate-binding domain (PubMed:20006620). The inactive D-stereoisomer binds in mirror symmetry with respect to the catalytic axis, revealing absolute stereospecificity of hydrogen transfer to the flavin (PubMed:21383134).
Induced in the presence of L-nicotine and D-nicotine (PubMed:5849820). Expressed during logarithmic growth and stationary phase (PubMed:4019415).
Inhibited by (R)-6-hydroxynicotine (PubMed:5849820, PubMed:4965794). Inhibited by high concentrations of phenanthroline (PubMed:5646150). Activity is strongly affected by Hg(2+) and p-chloromercuriphenylsulfonate, but not by N-ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoate) (PubMed:5646150).
Involved in the degradation of L-nicotine (PubMed:5849820). Catalyzes the oxidation of (S)-6-hydroxynicotine (6-hydroxy-L-nicotine) to 6-hydroxypseudooxynicotine (PubMed:5849820, PubMed:4965794, PubMed:5646150, PubMed:21383134, PubMed:26744768, PubMed:28080034). Oxidation of the pyrrolidine ring of (S)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine (PubMed:4965794, PubMed:21383134, PubMed:26744768, PubMed:28080034). Acts with absolute stereospecificity on the L-form of 6-hydroxynicotine (PubMed:4965794). Can also use (S)-6-hydroxynornicotine (PubMed:26744768, PubMed:28080034).
Catalyzes the oxidative demethylation of 4-methylaminobutanoate produced from the pyrrolidine ring of nicotine. To a much lesser extent, can also use sarcosine as substrate, but is not active against dimethylglycine, methylaminopropionitrile, methylaminopropylamine, and alpha-methylaminobutanoate.
Is transcribed only in the presence of nicotine under the control of the transcriptional activator PmfR. Forms part of an operon with purU, folD, nepA and nepB.
Aldehyde dehydrogenase able to oxidize various aldehydes such as formaldehyde, glyceraldehyde, butyraldehyde, glutaraldehyde and benzaldehyde (in vitro). Is likely involved in the oxidative D-xylose degradation pathway, catalyzing the oxidation step of 2-oxoglutarate semialdehyde to 2-oxoglutarate. Is able to use both NAD(+) and NADP(+); however, shows a preference for NADP(+). Does not display succinate semialdehyde dehydrogenase activity.
Belongs to the aldehyde dehydrogenase family.
Is induced by D-xylose. No significant levels of expression can detected when the cells are grown on various other sugars such as L-xylose, L-arabinose, D-galactose, D-tagatose and D-glucose.
Is constitutively expressed at low levels regardless of the presence or absence of D-xylose.
Transcriptional regulator involved in the regulation of the entire oxidative D-xylose degradation pathway via repression of the corresponding genes.
Catalyzes the conversion of 2,6-dihydroxypyridine into 2,3,6-trihydroxypyridine in the nicotine degradation pathway.
Belongs to the glycerate kinase type-1 family.
Heterohexamer of 2 alpha (kdhA), 2 beta (kdhB) and 2 gamma (kdhC) subunit. Dimer of heterotrimers (Probable).
Molybdo-flavoprotein enzyme complex involved in nicotine degradation. The subunit gamma (large subunit) contains the substrate-binding sites, the subunit alpha (medium subunit) binds FAD and the subunit beta (small subunit) has a 2Fe-2S ferredoxin-type domain which binds 2 2Fe-2S clusters.
Catalyzes the removal of methylamine from 4-methylaminobutanoate with the formation of succinate semialdehyde. Is involved in the catabolism of 4-methylaminobutanoate produced from nicotine. Has a very weak monoamine oxidase activity with 4-aminobutanoate. Cannot use spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyrate, methylamine propionitrile and methylamino propylamine as substrate.
Is transcribed only in the presence of nicotine.
Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).
Catalyzes the NADP(+)-dependent oxidation of D-xylose. Is able to use both NADP(+) and NAD(+); however, the enzyme shows a very strong preference for NADP(+). Is likely involved in the first step of the oxidative D-xylose degradation pathway.
Belongs to the sigma-70 factor family. ECF subfamily.
Belongs to the AB hydrolase superfamily.
L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6-dihydroxypseudooxynicotine.
Catalyzes the reduction of nicotine blue to its hydroquinone form. Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. May play a role in preventing the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. Besides nicotine blue, several other quinones are reduced by nboR.
Belongs to the glycosyl hydrolase 32 family.
Binds 1 Mo-molybdopterin cytosine dinucleotide (Mo-MCD) per subunit.
Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).
Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. NepA/NepB subfamily.
Component of an efflux pump responsible for the transport of nicotine breakdown products, in particular methylamine, out of the cell. This pump apparently serves as a metabolic valve for nicotine catabolites and may protect the bacteria from the potentially toxic side effects of these compounds.
Is transcribed only in the presence of nicotine under the control of the transcriptional activator PmfR. Forms part of an operon with nepB, folD, mabO and purU.
The efflux pump is composed of NepA and NepB.
Cells lacking this gene do not produce the MabO protein in the presence of nicotine, in contrast to the wild-type. Transcription of the nepAB genes and of the mao-ORF55-nbr operon is abolished in the mutant strain.
Forms oligomers in solution, probably homotetramers.
Transcriptional regulator involved in the activation of the purU-mabO-folD-nepA-nepB and mao-ORF55-nbr operons implicated in the nicotine catabolic pathway. The sequence GTTT-14 bp-AAAC seems to be the core binding site of the regulator upstream of the -35 promoter region of the operon.