29320:JMY29 RS20560
gene id: 29320:JMY29_RS20560
uniprot id: Q8GAI4
names: Function
names: Similarity
names: Subunit
messages: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
messages: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.
messages: Homodimer.

29320:JMY29 RS20210
gene id: 29320:JMY29_RS20210
uniprot id: Q8GAC3
names: Similarity
messages: Belongs to the site-specific recombinase resolvase family.

29320:JMY29 RS20690
gene id: 29320:JMY29_RS20690
uniprot id: Q93NH4
names: Activity Regulation
names: Cofactor
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the flavin monoamine oxidase family.
messages: Binds 1 FAD per subunit.
messages: Consists of two domains, an FAD-binding domain and a substrate-binding domain (PubMed:20006620). The inactive D-stereoisomer binds in mirror symmetry with respect to the catalytic axis, revealing absolute stereospecificity of hydrogen transfer to the flavin (PubMed:21383134).
messages: Cytoplasm
messages: Homodimer.
messages: Induced in the presence of L-nicotine and D-nicotine (PubMed:5849820). Expressed during logarithmic growth and stationary phase (PubMed:4019415).
messages: Inhibited by (R)-6-hydroxynicotine (PubMed:5849820, PubMed:4965794). Inhibited by high concentrations of phenanthroline (PubMed:5646150). Activity is strongly affected by Hg(2+) and p-chloromercuriphenylsulfonate, but not by N-ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoate) (PubMed:5646150).
messages: Involved in the degradation of L-nicotine (PubMed:5849820). Catalyzes the oxidation of (S)-6-hydroxynicotine (6-hydroxy-L-nicotine) to 6-hydroxypseudooxynicotine (PubMed:5849820, PubMed:4965794, PubMed:5646150, PubMed:21383134, PubMed:26744768, PubMed:28080034). Oxidation of the pyrrolidine ring of (S)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine (PubMed:4965794, PubMed:21383134, PubMed:26744768, PubMed:28080034). Acts with absolute stereospecificity on the L-form of 6-hydroxynicotine (PubMed:4965794). Can also use (S)-6-hydroxynornicotine (PubMed:26744768, PubMed:28080034).

29320:JMY29 RS20565
gene id: 29320:JMY29_RS20565
uniprot id: Q8GAI3
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the GcvT family.
messages: Binds 1 FAD covalently per subunit.
messages: Catalyzes the oxidative demethylation of 4-methylaminobutanoate produced from the pyrrolidine ring of nicotine. To a much lesser extent, can also use sarcosine as substrate, but is not active against dimethylglycine, methylaminopropionitrile, methylaminopropylamine, and alpha-methylaminobutanoate.
messages: Is transcribed only in the presence of nicotine under the control of the transcriptional activator PmfR. Forms part of an operon with purU, folD, nepA and nepB.

29320:JMY29 RS20450
gene id: 29320:JMY29_RS20450
uniprot id: Q8GAK7
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Aldehyde dehydrogenase able to oxidize various aldehydes such as formaldehyde, glyceraldehyde, butyraldehyde, glutaraldehyde and benzaldehyde (in vitro). Is likely involved in the oxidative D-xylose degradation pathway, catalyzing the oxidation step of 2-oxoglutarate semialdehyde to 2-oxoglutarate. Is able to use both NAD(+) and NADP(+); however, shows a preference for NADP(+). Does not display succinate semialdehyde dehydrogenase activity.
messages: Belongs to the aldehyde dehydrogenase family.
messages: Is induced by D-xylose. No significant levels of expression can detected when the cells are grown on various other sugars such as L-xylose, L-arabinose, D-galactose, D-tagatose and D-glucose.
messages: Monomer.

29320:JMY29 RS20425
gene id: 29320:JMY29_RS20425
uniprot id: Q8GAL2
names: Subcellular Location Annotation
messages: Membrane

29320:JMY29 RS20415
gene id: 29320:JMY29_RS20415
uniprot id: Q8GAL4
names: Function
names: Induction
messages: Is constitutively expressed at low levels regardless of the presence or absence of D-xylose.
messages: Transcriptional regulator involved in the regulation of the entire oxidative D-xylose degradation pathway via repression of the corresponding genes.

29320:JMY29 RS20575
gene id: 29320:JMY29_RS20575
uniprot id: Q8GAI0
names: Subcellular Location Annotation
messages: Membrane

29320:JMY29 RS20760
gene id: 29320:JMY29_RS20760
uniprot id: Q8GAG5
names: Subcellular Location Annotation
messages: Membrane

29320:JMY29 RS20630
gene id: 29320:JMY29_RS20630
uniprot id: Q93NG3
names: Function
names: Subunit
messages: Catalyzes the conversion of 2,6-dihydroxypyridine into 2,3,6-trihydroxypyridine in the nicotine degradation pathway.
messages: Homodimer.

29320:JMY29 RS20465
gene id: 29320:JMY29_RS20465
uniprot id: Q8GAK4
names: Similarity
messages: Belongs to the glycerate kinase type-1 family.

29320:JMY29 RS20775
gene id: 29320:JMY29_RS20775
uniprot id: Q8GAG2
names: Subcellular Location Annotation
messages: Membrane

29320:JMY29 RS20670
gene id: 29320:JMY29_RS20670
uniprot id: O87681
names: Cofactor
names: Function
names: Subunit
messages: Binds 1 FAD per subunit.
messages: Heterohexamer of 2 alpha (kdhA), 2 beta (kdhB) and 2 gamma (kdhC) subunit. Dimer of heterotrimers (Probable).
messages: Molybdo-flavoprotein enzyme complex involved in nicotine degradation. The subunit gamma (large subunit) contains the substrate-binding sites, the subunit alpha (medium subunit) binds FAD and the subunit beta (small subunit) has a 2Fe-2S ferredoxin-type domain which binds 2 2Fe-2S clusters.

29320:JMY29 RS20265
gene id: 29320:JMY29_RS20265
uniprot id: Q8GAB1
names: Similarity
messages: Belongs to the DprA/Smf family.

29320:JMY29 RS20535
gene id: 29320:JMY29_RS20535
uniprot id: Q8GAJ0
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the flavin monoamine oxidase family.
messages: Catalyzes the removal of methylamine from 4-methylaminobutanoate with the formation of succinate semialdehyde. Is involved in the catabolism of 4-methylaminobutanoate produced from nicotine. Has a very weak monoamine oxidase activity with 4-aminobutanoate. Cannot use spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyrate, methylamine propionitrile and methylamino propylamine as substrate.
messages: Is transcribed only in the presence of nicotine.
messages: Monomer.

29320:JMY29 RS20570
gene id: 29320:JMY29_RS20570
uniprot id: Q8GAI2
names: Function
names: Similarity
messages: Belongs to the PurU family.
messages: Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).

29320:JMY29 RS20675
gene id: 29320:JMY29_RS20675
uniprot id: O87682
names: Cofactor
names: Function
names: Subunit
messages: Binds 2 [2Fe-2S] clusters.
messages: Heterohexamer of 2 alpha (kdhA), 2 beta (kdhB) and 2 gamma (kdhC) subunit. Dimer of heterotrimers (Probable).
messages: Molybdo-flavoprotein enzyme complex involved in nicotine degradation. The subunit gamma (large subunit) contains the substrate-binding sites, the subunit alpha (medium subunit) binds FAD and the subunit beta (small subunit) has a 2Fe-2S ferredoxin-type domain which binds 2 2Fe-2S clusters.

29320:JMY29 RS20455
gene id: 29320:JMY29_RS20455
uniprot id: Q8GAK6
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the Gfo/Idh/MocA family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Catalyzes the NADP(+)-dependent oxidation of D-xylose. Is able to use both NADP(+) and NAD(+); however, the enzyme shows a very strong preference for NADP(+). Is likely involved in the first step of the oxidative D-xylose degradation pathway.
messages: Homotetramer.
messages: Is induced by D-xylose. No significant levels of expression can detected when the cells are grown on various other sugars such as L-xylose, L-arabinose, D-galactose, D-tagatose and D-glucose.

29320:JMY29 RS20380
gene id: 29320:JMY29_RS20380
uniprot id: Q8GAM4
names: Similarity
messages: Belongs to the sigma-70 factor family. ECF subfamily.

29320:JMY29 RS20650
gene id: 29320:JMY29_RS20650
uniprot id: Q93NG6
names: Function
names: Similarity
names: Subunit
messages: Belongs to the AB hydrolase superfamily.
messages: Homodimer.
messages: L-nicotine is used as a growth substrate. Plays a role in nicotine catabolism by cleaving a C-C bond in 2,6-dihydroxypseudooxynicotine.

29320:JMY29 RS20595
gene id: 29320:JMY29_RS20595
uniprot id: Q93NF6
names: Function
names: Subunit
messages: Catalyzes the reduction of nicotine blue to its hydroquinone form. Nicotine blue is the name given to the compound formed by the autocatalytic condensation of two molecules of 2,3,6-trihydroxypyridine, an intermediate in the nicotine degradation pathway. May play a role in preventing the intracellular formation of nicotine blue semiquinone radicals, which by redox cycling would lead to the formation of toxic reactive oxygen species. Besides nicotine blue, several other quinones are reduced by nboR.
messages: Homotetramer.

29320:JMY29 RS20290
gene id: 29320:JMY29_RS20290
uniprot id: Q8GAP2
names: Similarity
messages: Belongs to the glycosyl hydrolase 32 family.

29320:JMY29 RS20645
gene id: 29320:JMY29_RS20645
uniprot id: Q933N0
names: Cofactor
names: Function
names: Subunit
messages: Binds 1 Mo-molybdopterin cytosine dinucleotide (Mo-MCD) per subunit.
messages: Heterohexamer of 2 alpha (kdhA), 2 beta (kdhB) and 2 gamma (kdhC) subunit. Dimer of heterotrimers (Probable).
messages: Molybdo-flavoprotein enzyme complex involved in nicotine degradation. The subunit gamma (large subunit) contains the substrate-binding sites, the subunit alpha (medium subunit) binds FAD and the subunit beta (small subunit) has a 2Fe-2S ferredoxin-type domain which binds 2 2Fe-2S clusters.

29320:JMY29 RS20430
gene id: 29320:JMY29_RS20430
uniprot id: Q8GAL1
names: Subcellular Location Annotation
messages: Membrane

29320:JMY29 RS20730
gene id: 29320:JMY29_RS20730
uniprot id: Q8GAH0
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MoaC family.
messages: Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).
messages: Homohexamer; trimer of dimers.

29320:JMY29 RS20555
gene id: 29320:JMY29_RS20555
uniprot id: Q8GAI5
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. NepA/NepB subfamily.
messages: Cell membrane
messages: Component of an efflux pump responsible for the transport of nicotine breakdown products, in particular methylamine, out of the cell. This pump apparently serves as a metabolic valve for nicotine catabolites and may protect the bacteria from the potentially toxic side effects of these compounds.
messages: Is transcribed only in the presence of nicotine under the control of the transcriptional activator PmfR. Forms part of an operon with nepB, folD, mabO and purU.
messages: The efflux pump is composed of NepA and NepB.

29320:JMY29 RS20580
gene id: 29320:JMY29_RS20580
uniprot id: Q8GAH9
names: Disruption Phenotype
names: Function
names: Subunit
messages: Cells lacking this gene do not produce the MabO protein in the presence of nicotine, in contrast to the wild-type. Transcription of the nepAB genes and of the mao-ORF55-nbr operon is abolished in the mutant strain.
messages: Forms oligomers in solution, probably homotetramers.
messages: Transcriptional regulator involved in the activation of the purU-mabO-folD-nepA-nepB and mao-ORF55-nbr operons implicated in the nicotine catabolic pathway. The sequence GTTT-14 bp-AAAC seems to be the core binding site of the regulator upstream of the -35 promoter region of the operon.