224308:BSU 34990
gene id: 224308:BSU_34990
uniprot id: A0A6M3ZH24
uniprot id: O34752
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Lgt family.
messages: Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.
messages: Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins. Required for spore germination.
messages: Cell membrane

224308:BSU 28630
gene id: 224308:BSU_28630
uniprot id: A0A6M4JKJ5
uniprot id: P17922
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.
messages: Binds 2 magnesium ions per tetramer.
messages: Cytoplasm
messages: Tetramer of two alpha and two beta subunits.

224308:BSU 40400
gene id: 224308:BSU_40400
uniprot id: A0A6M4JR07
uniprot id: Q45614
names: Developmental Stage
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Autophosphorylated.
messages: Cell membrane
messages: Expressed during exponential growth and shut down at the entry into stationary phase.
messages: Homodimer. Interacts with YycH and YycI.
messages: Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Phosphorylates WalR.
messages: Membrane
messages: The imidazole derivatives NH125, NH126 and NH127 inhibited the incorporation of phosphate from ATP at 50 ug/ml. The zerumbone derivative NH0891 inhibited WalK.

224308:BSU 12680
gene id: 224308:BSU_12680
uniprot id: P54334
names: Similarity
messages: To B.subtilis YqbO.

224308:BSU 29280
gene id: 224308:BSU_29280
uniprot id: A0A6M4JL10
uniprot id: O34430
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.
messages: Cell membrane
messages: Membrane

224308:BSU 12190
gene id: 224308:BSU_12190
uniprot id: C0SPC3
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the Nudix hydrolase family.
messages: Cells lacking this gene have an unchanged spontaneous mutation frequency, even in triple mutT/yjhB/yvcI disruptions.
messages: Probably mediates the hydrolysis of some nucleoside diphosphate derivatives.

224308:BSU 14560
gene id: 224308:BSU_14560
uniprot id: A0A6M4JI86
uniprot id: Q45495
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the polypeptide deformylase family.
messages: Binds 1 Fe(2+) ion.
messages: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.

224308:BSU 34100
gene id: 224308:BSU_34100
uniprot id: O07015
names: Function
names: Induction
names: Similarity
messages: Belongs to the AB hydrolase superfamily.
messages: Induced by entry into stationary phase, but not by carbon and energy starvation.
messages: Positive regulator required for energy stress activation of the sigma-B transcription factor. Could be required for RsbP phosphatase activity.

224308:BSU 29440
gene id: 224308:BSU_29440
uniprot id: A0A6M3ZFN6
uniprot id: O34858
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.
messages: Cytoplasm

224308:BSU 07710
gene id: 224308:BSU_07710
uniprot id: A0A6M3Z8T2
uniprot id: O34952
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LTA synthase family.
messages: Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate.
messages: Cell membrane
messages: Membrane
messages: Proteolytically cleaved.
messages: Restores staphylococcal growth after ltaS depletion.
messages: Secreted

224308:BSU 18660
gene id: 224308:BSU_18660
uniprot id: A0A6M4JIT9
uniprot id: O34906
names: Domain
names: Function
names: Similarity
messages: Belongs to the SOS response-associated peptidase family.
messages: Glu-106 is involved in sensing abasic sites in single-stranded DNA (ssDNA). His-163 stabilizes the abasic sites by forming a hydrogen bond with the O4' hydroxyl group.
messages: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity).

224308:BSU 05490
gene id: 224308:BSU_05490
uniprot id: P96693
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the extradiol ring-cleavage dioxygenase family.
messages: Binds 1 Fe(2+) ion.
messages: Cytoplasm
messages: Putative ring-cleavage dioxygenase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 04520
gene id: 224308:BSU_04520
uniprot id: A0A6M4JFN7
uniprot id: P96608
names: Induction
names: Similarity
messages: Belongs to the acyl-CoA dehydrogenase family.
messages: Induced by methionine. Repressed by sulfate via the cysteine metabolism repressor YrzC/CymR.

224308:BSU 10950
gene id: 224308:BSU_10950
uniprot id: A0A6M3Z9I2
uniprot id: O06739
names: Function
names: Similarity
messages: Belongs to the phosphosulfolactate synthase family.
messages: Catalyzes the addition of sulfite to phosphoenolpyruvate (PEP) to yield (2R)-phospho-3-sulfolactate (PSL) (By similarity). Is probably involved in the biosynthesis of L-sulfolactate, which is a major constituent of sporulating cells and mature spores.

224308:BSU 24760
gene id: 224308:BSU_24760
uniprot id: P54504
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Cells lacking this gene have no visible phenotype is response to salt, ethanol or energy stress. However cells with multiple disruption (RsbRA, RsbRB and RsbRD) have an increased basal level of sigma-B, indicating this protein is a negative regulator of sigma-B.
messages: Negative regulator of sigma-B activity. Non-phosphorylated RsbS binds to RsbT, preventing its association with RsbU. Requires any one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and the RsbR paralog(s) are phosphorylated, they release RsbT, which can then bind and activate RsbU.
messages: One of 4 functionally non-identical RsbR paralogs, it functions in the environmental signaling branch of the general stress response.
messages: Phosphorylated by RsbT.
messages: Probably present in the stressosome with RsbRA, RsbRB, RsbRC and RsbS.

224308:BSU 10250
gene id: 224308:BSU_10250
uniprot id: O07608
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LplA family.
messages: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Is also able to use octanoate as substrate.
messages: Cells lacking this gene grow normally in minimal medium in the absence of supplements and have a wild-type pattern of lipoylated proteins. However, a double mutant strain lacking both lplJ and lipM is unable to grow in minimal medium either in the presence or in the absence of lipoic acid, indicating that LplJ is the sole B.subtilis lipoic acid salvage enzyme.
messages: Cytoplasm
messages: In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.

224308:BSU 15380
gene id: 224308:BSU_15380
uniprot id: A0A6M3ZE36
uniprot id: O31727
names: Function
names: Similarity
messages: Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.
messages: Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.

224308:BSU 38460
gene id: 224308:BSU_38460
uniprot id: A0A6M4JQJ9
uniprot id: P25151
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.
messages: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
messages: Cytoplasm
messages: Homodimer.

224308:BSU 04610
gene id: 224308:BSU_04610
uniprot id: A0A6M4JFP5
uniprot id: P96617
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S54 family.
messages: Cell membrane
messages: Membrane

224308:BSU 31240
gene id: 224308:BSU_31240
uniprot id: A0A6M3ZJ53
uniprot id: P39214
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpA is required for taxis towards glucose and alpha-methylglucoside.
messages: Deamidated by CheD on Gln-593 and Gln-594, producing glutamate residues. The glutamate residues are then methylated. Other additional sites are deamidated and methylated as well.
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft

224308:BSU 08330
gene id: 224308:BSU_08330
uniprot id: A0A6M4JGN1
uniprot id: P94442
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-2 integral membrane protein family.
messages: Cell membrane
messages: Induced in response to linearmycins and other polyenes via the two-component regulatory system LnrJ/LnrK.
messages: Membrane
messages: Required for resistance to linearmycins, a family of antibiotic-specialized metabolites produced by some streptomycetes (PubMed:26647299, PubMed:28461449). Part of the ABC transporter complex LnrLMN that probably facilitates linearmycin removal from the membrane. Responsible for the translocation of the substrate across the membrane (PubMed:28461449). Also mediates KinC-dependent biofilm morphology (PubMed:28461449).
messages: The complex is composed of two ATP-binding proteins (LnrL) and two transmembrane proteins (LnrM and LnrN).

224308:BSU 40300
gene id: 224308:BSU_40300
uniprot id: O32294
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rap family.
messages: Cytoplasm
messages: Disruption of the gene results in the enhancement of aprE expression.
messages: Inhibited by PhrG.
messages: Involved in the regulation of expression of DegU-controlled genes (PubMed:12950930). Inhibits the binding of DegU to the promoter regions of aprE, coding for an extracellular alkaline protease, and comK, a master regulator for development of genetic competence (PubMed:12950930). RapG does not stimulate dephosphorylation of DegU-P (PubMed:12950930).
messages: Part of the rapG-phrG operon (PubMed:12950930). Expression shows a slight decrease in stationary phase (PubMed:12950930). Repressed by RghR (PubMed:16553878).

224308:BSU 18340
gene id: 224308:BSU_18340
uniprot id: A0A6M3ZB81
uniprot id: P39845
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 2 phosphopantetheines covalently.
messages: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Orn as part of the biosynthesis of the lipopeptide antibiotic lipastatin. The Orn residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.

224308:BSU 03982
gene id: 224308:BSU_03982
uniprot id: C0H3V2
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II CmtAB PTS system is involved in D-mannitol transport.

224308:BSU 38410
gene id: 224308:BSU_38410
uniprot id: A0A6M4JQH1
uniprot id: P15400
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: Negatively regulates SacY activity by catalyzing its phosphorylation on 'His-99'.
messages: Negatively regulates SacY.
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIB type-2 domain may serve a regulatory function, through its phosphorylation activity.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system may be involved in sucrose transport.

224308:BSU 15470
gene id: 224308:BSU_15470
uniprot id: A0A6M3ZAL2
uniprot id: P39765
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.
messages: Homodimer and homohexamer; in equilibrium.
messages: Mutagenesis studies identified four amino acid residues that seem to be involved directly in binding of the protein to pyr mRNA: Thr-18, His-22, Arg-141 and Arg-146. Arg-27 and Lys-152 were also likely to be involved in RNA-binding, but mutations may have altered their subunit-subunit interactions. Arg-19 was implicated in pyr regulation, but a specific role in RNA-binding could not be demonstrated.
messages: Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes.
messages: UMP and UTP increase the affinity of PyrR for RNA.

224308:BSU 27750
gene id: 224308:BSU_27750
uniprot id: O05391
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Forespore intermembrane space
messages: Inhibits the SpoIVB zymogen from undergoing autocatalytic activation by an unknown mechanism, and in this way plays a role in the sigma-K checkpoint of sporulation.
messages: Monomer.

224308:BSU 38020
gene id: 224308:BSU_38020
uniprot id: A0A6M4JQD5
uniprot id: P39610
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ThiD family.
messages: Homodimer.
messages: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate.
messages: Was originally annotated in the complete genome as thiD.

224308:BSU 03960
gene id: 224308:BSU_03960
uniprot id: P94433
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cells lacking this gene show little or no difference in growth under copper deprivation, whereas possessing enhanced growth under copper excess conditions. Possesses a high intracellular content of copper.
messages: Cytoplasm
messages: May act as a negative transcriptional regulator of ycnJ in the presence of copper. May use copper as a corepressor.
messages: Significantly induced under copper-limiting conditions.

224308:BSU 11055
gene id: 224308:BSU_11055
uniprot id: A0A6M3Z9J5
uniprot id: O06750
names: Caution
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: Was initially thought to be two separate ORFs named yitO and yitN.

224308:BSU 11700
gene id: 224308:BSU_11700
uniprot id: O31619
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the HesA/MoeB/ThiF family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS.

224308:BSU 32300
gene id: 224308:BSU_32300
uniprot id: A0A6M3ZGJ8
uniprot id: O32126
names: Function
names: Similarity
names: Subunit
messages: Belongs to the HepT RNase toxin family.
messages: Homodimer, probably forms a complex with cognate antitoxin YutD.
messages: Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin YutD.

224308:BSU 18480
gene id: 224308:BSU_18480
uniprot id: A0A6M3ZGR5
uniprot id: P0CI77
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the pyrroline-5-carboxylate reductase family.
messages: Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.
messages: Cytoplasm
messages: The proG proH proI triple mutant is auxotrophic for proline.

224308:BSU 02340
gene id: 224308:BSU_02340
uniprot id: A0A6M4JCS5
uniprot id: P39817
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.
messages: Catalyzes the proton-dependent, binding-protein-independent transport of glutamate and aspartate.
messages: Cell membrane
messages: Glutamate uptake is inhibited by beta-hydroxyaspartate and cysteic acid.
messages: Membrane

224308:BSU 34150
gene id: 224308:BSU_34150
uniprot id: A0A6M3ZGS1
uniprot id: O32261
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Involved in galactan degradation (PubMed:27501980). Part of the ABC transporter complex GanPQS involved in the uptake of galactooligosaccharides (PubMed:27501980, PubMed:29240795). Responsible for the translocation of the substrate across the membrane (Probable).
messages: Membrane
messages: Repressed by the transcriptional regulator GanR and induced by galactobiose. Also repressed by glucose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (GanP and GanQ) and a solute-binding protein (GanS).

224308:BSU 14230
gene id: 224308:BSU_14230
uniprot id: O31699
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Monomer.
messages: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.

224308:BSU 28080
gene id: 224308:BSU_28080
uniprot id: A0A6M3ZET1
uniprot id: Q05865
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the folylpolyglutamate synthase family.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate, leading to folylpolyglutamate derivatives.
messages: Monomer.

224308:BSU 16800
gene id: 224308:BSU_16800
uniprot id: A0A6M3ZB79
uniprot id: P21458
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsK/SpoIIIE/SftA family.
messages: Cell membrane
messages: Cells lacking this gene show a chromosome translocation defect and aberrant compartmentalization of both sigma-F and sigma-E, and fail to complete membrane fusion at the end of engulfment.
messages: Consists of an N-terminal domain, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. Specific interactions between the gamma subdomain and specific SpoIIIE recognition sequences (SRS) regulate the compartment-specific activation of a mother-cell SpoIIIE complex. Interactions with nonpermissive SRS in the forespore lead to inactivation of the complex.
messages: Homohexamer. Forms a ring that surrounds DNA. Assembles into complexes that could contain two hexamers.
messages: Plays an essential role during sporulation. Required for the translocation of the chromosomal DNA from mother cell into the forespore during polar septation, for the final steps of compartmentalization in the presence of trapped DNA, and for the final steps of engulfment. The N-terminus mediates localization to the division septum and is required for both septal membrane fusion and engulfment membrane fusion. May form DNA-conducting channels across the two lipid bilayers of the septum after cell division. The C-terminus functions as a DNA motor that exports DNA in an ATP-dependent manner from mother cell into the forespore. DNA-binding proteins are stripped off the chromosome during translocation, which may play a key role in reprogramming developmental gene expression in the forespore. The two arms of the chromosome are simultaneously pumped into the forespore, which suggests that the septum contains at least two channels, one for each arm. Required for separation of chromosome termini. Also required for optimal chromosome partitioning in vegetative cells, by actively moving chromosomal DNA trapped within the division septum into the daughter cells.

224308:BSU 31000
gene id: 224308:BSU_31000
uniprot id: O32075
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 32330
gene id: 224308:BSU_32330
uniprot id: A0A6M4JLV8
uniprot id: O32129
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the radical SAM superfamily. Lipoyl synthase family.
messages: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.
messages: Cytoplasm
messages: Disruption of this gene interrupts lipoate-dependent reactions, which strongly inhibits growth in minimal medium, impairing the generation of branched-chain fatty acids and leading to accumulation of copious amounts of straight-chain saturated fatty acids in B.subtilis membranes.

224308:BSU 16160
gene id: 224308:BSU_16160
uniprot id: A0A6M3ZAR6
uniprot id: P39778
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A double ring-shaped homohexamer of ClpQ is capped on each side by a ring-shaped ClpY homohexamer. The assembly of the ClpQ/ClpY complex is dependent on binding of ATP (By similarity).
messages: A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.
messages: ATPase activity is much induced upon complex formation with ClpQ.
messages: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity.
messages: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.
messages: Belongs to the ClpX chaperone family. HslU subfamily.
messages: Cytoplasm

224308:BSU 09830
gene id: 224308:BSU_09830
uniprot id: O07539
names: Induction
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.

224308:BSU 27430
gene id: 224308:BSU_27430
uniprot id: A0A6M4JLG5
uniprot id: O34677
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Part of the ABC transporter complex GlnHMPQ involved in glutamine transport. Probably responsible for energy coupling to the transport system (By similarity).
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: The complex is composed of two ATP-binding proteins (GlnQ), two transmembrane proteins (GlnM and GlnP) and a solute-binding protein (GlnH).

224308:BSU 36730
gene id: 224308:BSU_36730
uniprot id: P70961
names: Similarity
messages: To B.subtilis YwmC.

224308:BSU 31930
gene id: 224308:BSU_31930
uniprot id: A0A6M3ZG60
uniprot id: Q08352
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A monocistronic operon. Induced by L-alanine in minimal media. Constitutively expressed in rich medium, declines during sporulation. Induced early during sporulation on minimal medium by L-alanine. Affects its own expression (PubMed:8226620). Transcription activated by AdeR (PubMed:22797752).
messages: Belongs to the AlaDH/PNT family.
messages: Catalyzes the reversible oxidative deamination of L-alanine to pyruvate. Oxidative deamination proceeds through a sequential, ordered ternary-binary mechanism, where NAD(+) binds first followed by L-alanine; the products are released in the order ammonia, pyruvate and NADH (PubMed:6794611, PubMed:6794612) (Probable). Disruption blocks sporulation probably in stage V; 20-30% sporulation can be restored if the media is supplemented with pyruvate, suggesting lack of pyruvate blocks sporulation. Thus it is a key factor in the assimilation of L-alanine as an energy source via the tricarboxylic acid cycle during sporulation (PubMed:8226620).
messages: Cytoplasm
messages: Homohexamer. Trimer of dimer (By similarity).
messages: Strain cannot grow on L-alanine as a sole carbon source. Disruption of ald causes a sporulation defect, most likely in stage V.

224308:BSU 04800
gene id: 224308:BSU_04800
uniprot id: P96629
names: Function
names: Similarity
messages: Belongs to the 'phage' integrase family.
messages: Putative integrase that is involved in the insertion of the integrative and conjugative element ICEBs1. Required for the excision of ICEBs1 from the donor cell genome and subsequent integration in the recipient cell genome. Appears not to be transferred through the mating pore. Integration of ICEBs1 involves an attachment site in the chromosome, attB, and a site in the circular form of ICEBs1, attICEBs1.

224308:BSU 11430
gene id: 224308:BSU_11430
uniprot id: A0A6M4JM05
uniprot id: P24141
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 5 family.
messages: Cell membrane
messages: Interacts with FloT in detergent-resistant membranes (DRM) (PubMed:23651456). Colocalizes rarely with FloT membrane assemblies (PubMed:27362352).
messages: Membrane
messages: Membrane raft
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence.

224308:BSU 38970
gene id: 224308:BSU_38970
uniprot id: P42317
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 23930
gene id: 224308:BSU_23930
uniprot id: A0A6M4JIY1
uniprot id: P54540
names: Similarity
messages: Belongs to the methylmalonyl-CoA epimerase family.

224308:BSU 07910
gene id: 224308:BSU_07910
uniprot id: A0A6M4JG00
uniprot id: O34929
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 36250
gene id: 224308:BSU_36250
uniprot id: A0A6M4JPX7
uniprot id: P96716
names: Function
names: PTM
names: Similarity
messages: Autophosphorylated in vitro, which inhibits ATPase activity. Dephosphorylated by YwqE in vitro.
messages: Belongs to the CpsD/CapB family.
messages: May be involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylates in vitro. Phosphorylates and activates in vitro two UDP-glucose dehydrogenases, YwqF and TuaD, as well as the DNA-binding proteins Ssb and SsbB.

224308:BSU 31130
gene id: 224308:BSU_31130
uniprot id: A0A6M4JLJ4
uniprot id: O32084
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 10100
gene id: 224308:BSU_10100
uniprot id: P38049
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the antibiotic biosynthesis monooxygenase family.
messages: Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
messages: Constitutively expressed.
messages: Cytoplasm
messages: Homodimer.
messages: Neither hmoB single mutant nor the hmoA hmoB double mutant display robust and reproducible phenotypes relative to the wild-type.

224308:BSU 01340
gene id: 224308:BSU_01340
uniprot id: A0A6M3Z6Z3
uniprot id: P19947
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL30 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 15360
gene id: 224308:BSU_15360
uniprot id: O31725
names: Developmental Stage
names: Disruption Phenotype
names: Induction
names: Similarity
messages: Belongs to the YlmC/YmxH family.
messages: Expressed in the mother cell during sporulation.
messages: Expression is regulated by the sporulation transcription factor sigma E.
messages: Inactivation of the gene does not affect sporulation, but a ylmC/ymxH double mutant shows a 100-fold reduction in the efficiency of sporulation.

224308:BSU 34350
gene id: 224308:BSU_34350
uniprot id: A0A6M3ZIZ9
uniprot id: P71052
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide synthase family.
messages: Cell membrane
messages: Involved in biofilm formation.

224308:BSU 19360
gene id: 224308:BSU_19360
uniprot id: A0A6M4JH74
uniprot id: P16263
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the 2-oxoacid dehydrogenase family.
messages: Binds 1 lipoyl cofactor covalently.
messages: E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).
messages: Forms a 24-polypeptide structural core with octahedral symmetry. Part of the 2-oxoglutarate dehydrogenase (OGDH) complex composed of E1 (2-oxoglutarate dehydrogenase), E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase); the complex contains multiple copies of the three enzymatic components (E1, E2 and E3).

224308:BSU 26060
gene id: 224308:BSU_26060
uniprot id: P45929
names: Similarity
messages: To B.subtilis XkdM.

224308:BSU 37410
gene id: 224308:BSU_37410
uniprot id: P71007
names: Function
names: Induction
messages: Involved in the production of the bacteriocin subtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 31990
gene id: 224308:BSU_31990
uniprot id: A0A6M3ZFN3
uniprot id: P45744
names: Similarity
messages: Belongs to the isochorismate synthase family.

224308:BSU 35720
gene id: 224308:BSU_35720
uniprot id: A0A6M3ZGV1
uniprot id: P13485
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CDP-glycerol glycerophosphotransferase family.
messages: Cell membrane
messages: Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.
messages: Responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the disaccharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in length.

224308:BSU 22980
gene id: 224308:BSU_22980
uniprot id: P50733
names: Induction
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 02510
gene id: 224308:BSU_02510
uniprot id: A0A6M4JF31
uniprot id: P42240
names: Function
names: Similarity
messages: Belongs to the UxaA family.
messages: Catalyzes the dehydration of galactarate to form 5-dehydro-4-deoxy-D-glucarate (5-KDG).

224308:BSU 34460
gene id: 224308:BSU_34460
uniprot id: A0A6M4JPE5
uniprot id: O07003
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 32 family.
messages: Catalyzes the degradation of levan mainly into levanbiose (difructose). Is not active on sucrose.
messages: Cell membrane
messages: Induced by sucrose.

224308:BSU 25970
gene id: 224308:BSU_25970
uniprot id: P45936
names: Similarity
messages: To B.subtilis XkdU.

224308:BSU 18880
gene id: 224308:BSU_18880
uniprot id: A0A6M3ZBG6
uniprot id: O34915
names: Domain
names: Function
names: Similarity
messages: Belongs to the SOS response-associated peptidase family.
messages: Glu-106 is involved in sensing abasic sites in single-stranded DNA (ssDNA). His-163 stabilizes the abasic sites by forming a hydrogen bond with the O4' hydroxyl group.
messages: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity).

224308:BSU 28330
gene id: 224308:BSU_28330
uniprot id: P94562
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 04200
gene id: 224308:BSU_04200
uniprot id: P96578
names: Function
names: Similarity
names: Subunit
messages: Belongs to the D-lyxose ketol-isomerase family.
messages: Homodimer.
messages: Sugar isomerase that catalyzes the reversible isomerization of D-lyxose to D-xylulose.

224308:BSU 40760
gene id: 224308:BSU_40760
uniprot id: P37508
names: Similarity
names: Subcellular Location Annotation
messages: Membrane
messages: To B.subtilis YwjB.

224308:BSU 26360
gene id: 224308:BSU_26360
uniprot id: P45901
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 04090
gene id: 224308:BSU_04090
uniprot id: Q7WY77
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the PxpC family.
messages: Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate (PubMed:28830929). In addition, counteracts the inhibitory action of PxpB (KipI) on sporulation, by binding to PxpB and preventing its function as an inhibitor of kinase A (PubMed:9334321).
messages: Disruption of the gene decreases sporulation (PubMed:9334321). Deletion mutant grows less well than wild type on minimal medium with ammonium as nitrogen source and cannot grow on 5-oxoproline. Mutant lacks 5-oxoprolinase activity and accumulates 5-oxo-L-proline (PubMed:28830929).
messages: Forms a complex composed of PxpA, PxpB and PxpC (PubMed:28830929). Interacts with PxpB (PubMed:9334321, PubMed:21050859, PubMed:28830929).
messages: Induced by glucose when readily available sources of nitrogen, such as ammonia or glutamine, are scarce. Transcriptionally activated by TnrA and repressed by KipR.
messages: Was originally (PubMed:8574415, PubMed:8969502) thought to be a longer ORF that encodes what is now known to be pxpB (kipI) and pxpC (kipA).

224308:BSU 06100
gene id: 224308:BSU_06100
uniprot id: O34885
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CTCGAG-3'; the cleavage site is unknown.
messages: BsuMI restriction activity requires YdiR, YdiS and YdjA.
messages: Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydjA.
messages: Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences (PubMed:11751814). Triple deletion ydiO-ydiP-ydiS leads to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).
messages: Not expressed during sporulation.

224308:BSU 00540
gene id: 224308:BSU_00540
uniprot id: P37553
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: An anti-sigma factor for sporulation specific sigma-F factor, by antagonizing sigma-F it allows the switch to sigma-G factor and progression to the late sporulation development stages (PubMed:21037003). Might stabilize or process Holliday junction intermediates, although this may be due to polar effects on the downstream mfd gene (PubMed:15317759).
messages: Cells missing this gene prolong the activity of sigma-F factor and do not fully induce the activity of sigma-G factor; sporulation efficiency is reduced 50-fold with most spores arresting in the engulfment stage (III) (PubMed:21037003). About 10-fold increased sensitivity to DNA damaging agents, 60% reduced chromosomal DNA transformation; plasmid transformation is unaffected. Partially suppresses DNA damaging agent sensitivity of recU deletions (PubMed:11810266). Partially suppresses DNA damaging agent sensitivity of recU deletions (PubMed:11810266). Partially suppresses DNA repair and chromosome segregation defects in ruvA and recG mutants cells, but not in recD or recU mutant cells (PubMed:15317759).
messages: Cytoplasm
messages: Diffusely expressed in the forespore throughout spore development.
messages: Induced at 2 hours after sporulation onset as part of the sigma-F regulon, followed by further induction after 3 hours as part of the sigma-G regulon; its concentration rises throughout sporulation (at protein level) (PubMed:21037003).
messages: Recombination phenotypes may be due to polar effects on the downstream gene mfd rather than due to loss of the anti-sigma factor (PubMed:21037003).

224308:BSU 32140
gene id: 224308:BSU_32140
uniprot id: P21341
names: Function
names: Similarity
messages: Belongs to the PaiB family.
messages: Involved in the reduction of extracellular and cell-associated protease levels, as well as in the reduced levels of alpha-amylase, levansucrase, alkaline phosphatase and sporulation inhibition, when present in high copy number.

224308:BSU 17000
gene id: 224308:BSU_17000
uniprot id: A0A6M3ZB08
uniprot id: O31777
names: Function
names: Similarity
names: Subunit
messages: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.
messages: Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.
messages: Homodimer.

224308:BSU 07600
gene id: 224308:BSU_07600
uniprot id: A0A6M3ZBQ4
uniprot id: O34439
names: Similarity
messages: Belongs to the UPF0065 (bug) family.

224308:BSU 36910
gene id: 224308:BSU_36910
uniprot id: A0A6M4JQ29
uniprot id: P39157
names: Induction
names: Similarity
messages: Belongs to the UPF0340 family.
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 13750
gene id: 224308:BSU_13750
uniprot id: A0A6M3ZAB3
uniprot id: O31678
names: Activity Regulation
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activity is strongly inhibited by Cu(2+) and Fe(3+).
messages: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.
messages: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a late step in the queuosine pathway.
messages: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
messages: Crystallographic structure revealed that QueF enzyme forms an asymmetric tunnel-fold homodecamer (PubMed:22787148) and not a homododecamer as originally proposed (PubMed:15767583).
messages: Cytoplasm
messages: Does not require a metal cofactor.
messages: Forms an asymmetric tunnel-fold homodecamer of two head-to-head facing pentamers, harboring 10 active sites at the intersubunit interfaces.

224308:BSU 08970
gene id: 224308:BSU_08970
uniprot id: P39134
names: Similarity
messages: Belongs to the PrkA family.

224308:BSU 29050
gene id: 224308:BSU_29050
uniprot id: O34678
names: Similarity
messages: Belongs to the aldo/keto reductase family.

224308:BSU 23510
gene id: 224308:BSU_23510
uniprot id: A0A6M4JNL9
uniprot id: P46352
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the 'phage' integrase family. XerD subfamily.
messages: Cytoplasm
messages: Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.
messages: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.

224308:BSU 26730
gene id: 224308:BSU_26730
uniprot id: A0A6M4JLH8
uniprot id: O07938
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the barstar family.
messages: Cytoplasm

224308:BSU 08820
gene id: 224308:BSU_08820
uniprot id: A0A3Q8S020
uniprot id: P26901
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the catalase family.
messages: By hydrogen peroxide.
messages: Cytoplasm
messages: Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
messages: Elevated levels of expression during entry into the stationary phase of the growth cycle.
messages: Insertion mutant shows increased sensitivity to H(2)0(2), reduced catalase expression and an absence of induced protection of the cell.

224308:BSU 01910
gene id: 224308:BSU_01910
uniprot id: O31422
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: By Spo0A (PubMed:12817086) and PhoP (PubMed:16816204), during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Produces a 26-residue extracellular sporulation filling factor (SKF) that induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation (PubMed:12817086, PubMed:20805502). Can also inhibit growth of other bacteria at high concentrations (PubMed:11851812). Addition of SKF to solid cultures induces killing, but it is much less effective than SDP (AC O34344) (PubMed:20805502). Has a role in protecting the secreted lipase LipA against proteolysis, either by modulating its folding or by acting as a protease inhibitor (PubMed:15812018).
messages: Secreted
messages: This is a cyclic peptide (PubMed:20805502, PubMed:23282011). The first step in SKF maturation is probably thioether bond formation (PubMed:23282011).
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086). In a single gene deletion no SKP is produced (PubMed:20805502).

224308:BSU 23740
gene id: 224308:BSU_23740
uniprot id: P54558
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 07780
gene id: 224308:BSU_07780
uniprot id: A0A6M4JJI9
uniprot id: O35028
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKC lipoprotein family.
messages: Cell membrane
messages: May be involved in spore germination.

224308:BSU 18470
gene id: 224308:BSU_18470
uniprot id: A0A6M4JJ88
uniprot id: O07509
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glutamate 5-kinase family.
messages: Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.
messages: Cytoplasm

224308:BSU 32260
gene id: 224308:BSU_32260
uniprot id: A0A6M3ZFZ8
uniprot id: P19582
names: Activity Regulation
names: Similarity
messages: Belongs to the homoserine dehydrogenase family.
messages: Feedback inhibition by threonine.

224308:BSU 22850
gene id: 224308:BSU_22850
uniprot id: P50742
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30840
gene id: 224308:BSU_30840
uniprot id: A0A6M3ZFD5
names: Caution
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 31980
gene id: 224308:BSU_31980
uniprot id: P40871
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Cytoplasm
messages: Involved in the biosynthesis of the catecholic siderophore bacillibactin. Catalyzes the activation of the carboxylate group of 2,3-dihydroxy-benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate.

224308:BSU 25820
gene id: 224308:BSU_25820
uniprot id: P45944
names: Similarity
messages: Belongs to the DcsA family.

224308:BSU 03920
gene id: 224308:BSU_03920
uniprot id: A0A6M4JGC9
uniprot id: P40420
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GRP transporter (TC 2.A.7.5) family.
messages: Cell membrane
messages: Expressed during sporulation.
messages: Involved in the uptake of glucose.
messages: Membrane

224308:BSU 31840
gene id: 224308:BSU_31840
uniprot id: O32099
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Cytoplasm
messages: Reduces benzil stereospecifically to (S)-benzoin.

224308:BSU 28180
gene id: 224308:BSU_28180
uniprot id: P40736
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31920
gene id: 224308:BSU_31920
uniprot id: A0A6M3ZJ27
uniprot id: P71073
names: Disruption Phenotype
names: Function
names: Similarity
messages: Activates ald expression in response to alanine availability and is important for normal sporulation in B.subtilis.
messages: Belongs to the CdaR family.
messages: Disruption of adeR causes a sporulation defect.

224308:BSU 31540
gene id: 224308:BSU_31540
uniprot id: A0A6M4JNA1
uniprot id: O05252
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A null mutation does not lead to any detectable defect in guanosine uptake. However, in the nupG-nupN double mutant, guanosine uptake is reduced to an almost undetectable level.
messages: Belongs to the BMP lipoprotein family.
messages: Cell membrane
messages: Membrane raft
messages: Part of an ABC transporter complex involved in the uptake of guanosine (PubMed:21926227). Is probably the substrate-binding protein of the system (PubMed:21926227). May be a nucleoside transporter of broad specificity but with various affinities for different substrates (PubMed:21926227).
messages: The complex is composed of two ATP-binding proteins (NupO), two transmembrane proteins (NupP and NupQ) and a solute-binding protein (NupN).
messages: Transcriptionally regulated by CodY.

224308:BSU 23519
gene id: 224308:BSU_23519
uniprot id: C0H451
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 33080
gene id: 224308:BSU_33080
uniprot id: A0A6M3ZGI7
uniprot id: O32197
names: Function
names: Induction
names: PTM
names: Subcellular Location Annotation
messages: Autoregulated. Induced by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.
messages: Cytoplasm
messages: Member of the two-component regulatory system LiaS/LiaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also involved in response to cationic antimicrobial peptides and secretion stress. LiaR regulates the transcription of the liaIHGFSR operon.
messages: Phosphorylated by LiaS.

224308:BSU 28740
gene id: 224308:BSU_28740
uniprot id: A0A6M3ZF96
uniprot id: P94529
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex AraNPQ involved in the uptake of arabinooligosaccharides. Transports alpha-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (PubMed:20693325). Responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (AraP and AraQ) and a solute-binding protein (AraN).
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 40990
gene id: 224308:BSU_40990
uniprot id: A0A6M3ZIB9
uniprot id: P37524
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ParB family.
messages: Effects nucleoid occlusion by binding relatively nonspecifically to DNA and preventing the assembly of the division machinery in the vicinity of the nucleoid, especially under conditions that disturb the cell cycle. It helps to coordinate cell division and chromosome segregation by preventing the formation of the Z ring through the nucleoid, which would cause chromosome breakage.
messages: Expressed constitutively.
messages: nucleoid

224308:BSU 28560
gene id: 224308:BSU_28560
uniprot id: P94547
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.

224308:BSU 15590
gene id: 224308:BSU_15590
uniprot id: A0A6M3ZAX3
uniprot id: O34764
names: Induction
names: Similarity
messages: Belongs to the sulfate adenylyltransferase family.
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 08170
gene id: 224308:BSU_08170
uniprot id: A0A6M3Z911
uniprot id: O31558
names: Similarity
messages: Belongs to the WXG100 family.

224308:BSU 29700
gene id: 224308:BSU_29700
uniprot id: P39066
names: Function
messages: Role in growth and sporulation on acetoin or butanediol. Involved in the breakdown of these compounds used as a carbon source.

224308:BSU 27120
gene id: 224308:BSU_27120
uniprot id: A0A6M4JLD6
uniprot id: O05404
names: Function
names: Similarity
names: Subunit
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: Interacts with RsiV.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of proteins involved in stress responses against bacitracin, paraquat and tellurite.

224308:BSU 35020
gene id: 224308:BSU_35020
uniprot id: A0A6M3ZGD5
uniprot id: O35000
names: Caution
names: Function
names: Induction
names: Similarity
messages: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.
messages: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.
messages: Expression is repressed by the HTH-type transcriptional regulator NagR.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 33690
gene id: 224308:BSU_33690
uniprot id: A0A6M3ZJE1
uniprot id: O32239
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation (By similarity).

224308:BSU 38990
gene id: 224308:BSU_38990
uniprot id: A0A6M4JQN0
uniprot id: P42315
names: Similarity
names: Subunit
messages: Belongs to the 3-oxoacid CoA-transferase subunit A family.
messages: Heterodimer of a subunit A and a subunit B.

224308:BSU 39830
gene id: 224308:BSU_39830
uniprot id: P46332
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 03180
gene id: 224308:BSU_03180
uniprot id: P94388
names: Biotechnology
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the carbohydrate esterase 7 family.
messages: Cytoplasm
messages: Esterase that removed acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan. Cannot cleave amide linkages.
messages: Homohexamer.
messages: Used in the pharmaceutical industry for the chemoenzymatic deacetylation of cephalosporins and the synthesis of novel antibiotics.

224308:BSU 39700
gene id: 224308:BSU_39700
uniprot id: P26935
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the Gfo/Idh/MocA family.
messages: By inositol. Subjected to catabolite repression.
messages: Homotetramer.
messages: Involved in the oxidation of myo-inositol (MI) and D-chiro-inositol (DCI) to 2-keto-myo-inositol (2KMI or 2-inosose) and 1-keto-D-chiro-inositol (1KDCI), respectively. Can also use D-glucose and D-xylose, and shows a trace of activity with D-ribose and D-fructose.

224308:BSU 16350
gene id: 224308:BSU_16350
uniprot id: A0A6M3ZB48
uniprot id: P35528
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliP/MopC/SpaP family.
messages: Cell membrane
messages: Membrane
messages: Plays a role in the flagellum-specific transport system.

224308:BSU 03090
gene id: 224308:BSU_03090
uniprot id: P94381
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rht family.
messages: Cell membrane

224308:BSU 01470
gene id: 224308:BSU_01470
uniprot id: A0A6M4JEH2
uniprot id: P70972
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Abolishes riboflavin uptake, cell growth less inhibited by roseoflavin, a toxic riboflavin analog.
messages: Belongs to the energy-coupling factor EcfT family.
messages: Cell membrane
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component). May be able to interact with more than 1 S component at a time (By similarity).
messages: Membrane
messages: Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (By similarity). Involved in riboflavin transport.
messages: Transmembrane (T) component of an energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.

224308:BSU 21810
gene id: 224308:BSU_21810
uniprot id: A0A6M3ZGH1
uniprot id: P11045
names: Function
names: Similarity
messages: Belongs to the dihydrofolate reductase family.
messages: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).
messages: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

224308:BSU 30870
gene id: 224308:BSU_30870
uniprot id: O34886
names: Similarity
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.

224308:BSU 38230
gene id: 224308:BSU_38230
uniprot id: P39600
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30659
gene id: 224308:BSU_30659
uniprot id: C0H3Q1
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05040
gene id: 224308:BSU_05040
uniprot id: A0A6M3Z7T0
uniprot id: P96651
names: Similarity
messages: To bacterial alkanal monooxygenase alpha and beta chains.

224308:BSU 04980
gene id: 224308:BSU_04980
uniprot id: P96646
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 34950
gene id: 224308:BSU_34950
uniprot id: A0A6M4JPI7
uniprot id: O34310
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide lyase 3 family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins.
messages: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins. Is also able to cleave trigalacturonate to galacturonic acid and unsaturated digalacturonate.
messages: Hg(2+) could replace calcium ion.
messages: Secreted

224308:BSU 35180
gene id: 224308:BSU_35180
uniprot id: P37953
names: Developmental Stage
names: Subcellular Location Annotation
messages: Cell membrane
messages: Maximally expressed in early stationary phase in medium containing glucose and glutamine.

224308:BSU 01250
gene id: 224308:BSU_01250
uniprot id: A0A6M3Z785
uniprot id: P12874
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS17 family.
messages: One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.
messages: Part of the 30S ribosomal subunit.

224308:BSU 27540
gene id: 224308:BSU_27540
uniprot id: O32037
names: Function
names: Similarity
messages: Belongs to the HesA/MoeB/ThiF family.
messages: Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.

224308:BSU 27960
gene id: 224308:BSU_27960
uniprot id: A0A6M4JKM8
uniprot id: P26908
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL21 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Contacts protein L20 (By similarity).
messages: Part of the 50S ribosomal subunit. Contacts protein L20.
messages: This protein binds to 23S rRNA in the presence of protein L20.

224308:BSU 10450
gene id: 224308:BSU_10450
uniprot id: A0A6M3Z946
uniprot id: O07556
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
messages: Cell membrane
messages: Membrane

224308:BSU 17460
gene id: 224308:BSU_17460
uniprot id: A0A6M3ZB07
uniprot id: P12425
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glutamine synthetase family.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Completely inhibited by glutamine and partially inhibited by glycine, alanine and AMP (PubMed:4149044, PubMed:24158439). Also inhibited by L-methionine-SR-sulphoximine (Met-Sox) (PubMed:8093698, PubMed:24158439).
messages: Cytoplasm
messages: Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism (PubMed:25691471). It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:24158439). Feedback-inhibited GlnA interacts with and regulates the activity of the transcriptional regulator TnrA (PubMed:11719184, PubMed:12139611). During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation (PubMed:11719184, PubMed:12139611, PubMed:25691471). Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity (PubMed:11719184, PubMed:12139611, PubMed:25691471). In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes (PubMed:25691471).
messages: In cells lacking this gene, expression of glnR, tnrA, nasB, nrgAB, gabP and ure genes is derepressed.
messages: Oligomer of 12 subunits arranged in the form of two hexagons.
messages: Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity. This inhibitory effect is the highest when both glutamine and AMP are present.
messages: Repressed by GlnR under conditions of nitrogen excess (PubMed:2573733). Repressed by TnrA under conditions of nitrogen limitation.

224308:BSU 04060
gene id: 224308:BSU_04060
uniprot id: P42964
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NRAMP family.
messages: Cell membrane

224308:BSU 40410
gene id: 224308:BSU_40410
uniprot id: A0A6M3ZIX1
uniprot id: P37478
names: Developmental Stage
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Expressed during exponential growth and shut down at the entry into stationary phase.
messages: Homodimer.
messages: Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Binds to the ftsAZ P1 promoter sequence in vitro. WalR has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.
messages: Phosphorylated by WalK.

224308:BSU 03670
gene id: 224308:BSU_03670
uniprot id: A0A6M4JIG0
uniprot id: P94408
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
messages: Cell membrane
messages: Membrane

224308:BSU 31560
gene id: 224308:BSU_31560
uniprot id: A0A6M3ZFJ9
uniprot id: O05254
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of an ABC transporter complex involved in the uptake of guanosine (PubMed:21926227). Responsible for the translocation of the substrate across the membrane (Probable). May be a nucleoside transporter of broad specificity but with various affinities for different substrates (PubMed:21926227).
messages: The complex is composed of two ATP-binding proteins (NupO), two transmembrane proteins (NupP and NupQ) and a solute-binding protein (NupN).
messages: Transcriptionally regulated by CodY.

224308:BSU 18980
gene id: 224308:BSU_18980
uniprot id: O34774
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 09290
gene id: 224308:BSU_09290
uniprot id: A0A6M4JGW5
uniprot id: P18157
names: Activity Regulation
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).
messages: Belongs to the FGGY kinase family.
messages: Homotetramer and homodimer (in equilibrium).
messages: Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.

224308:BSU 36720
gene id: 224308:BSU_36720
uniprot id: P70966
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 01950
gene id: 224308:BSU_01950
uniprot id: O31427
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: Belongs to the ABC transporter superfamily. SkfA peptide export (TC 3.A.1.128.1) family.
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Cell membrane
messages: Probably part of the ABC transporter SkfEF involved in the export of the bacteriocin SKF. Probably responsible for energy coupling to the transport system.
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 03750
gene id: 224308:BSU_03750
uniprot id: A0A6M3Z803
uniprot id: P94413
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Could be member of the two-component regulatory system YclK/YclJ.
messages: Cytoplasm
messages: Phosphorylated by YclK.

224308:BSU 04590
gene id: 224308:BSU_04590
uniprot id: P96615
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0699 family.
messages: Cell membrane

224308:BSU 07430
gene id: 224308:BSU_07430
uniprot id: O34750
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: A probable DEAD-box RNA helicase that plays a role in ribosomal 50S subunit assembly. May be a non-specific RNA helicase.
messages: Belongs to the DEAD box helicase family.
messages: In rich medium highest expression in exponential growth, expression decreases in stationary phase (at protein level).
messages: No visible effect at 37 degrees Celsius, decreased growth at 16 degrees Celsius. A quadruple disruption of all RNA helicases (cshA, cshB, deaD, yfmL) is not lethal at 37 degrees Celsius, although both 50S and 70S ribosomes are decreased, while growth stops at 16 degrees.

224308:BSU 09110
gene id: 224308:BSU_09110
uniprot id: A0A6M3Z999
uniprot id: P54594
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NlpA lipoprotein family.
messages: Cell membrane
messages: Membrane

224308:BSU 09150
gene id: 224308:BSU_09150
uniprot id: P54598
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expressed in the forespore during sporulation.
messages: Expression is dependent on sigma G, and to a much lesser extent on sigma F.
messages: Forespore inner membrane
messages: Mutant sporulates normally and has the same resistance to heat and UV radiation than wild-type spores. It exhibits no defect in the initiation of spore germination, but the spore outgrowth is slower than that of wild-type spores (PubMed:9611260). Mutant shows a reduced rate of spore germination in L-alanine (PubMed:28333204).
messages: Probably contributes, directly or indirectly, to early events in germination (PubMed:28333204). May play a role in spore outgrowth (PubMed:9611260).

224308:BSU 30440
gene id: 224308:BSU_30440
uniprot id: O34898
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-5 integral membrane protein family.
messages: Cell membrane
messages: Expressed early in the stationary phase.
messages: Negatively regulated by YtrA.
messages: Part of the ABC transporter complex YtrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.
messages: The complex is composed of 2 ATP-binding proteins (YtrB and YtrE), 2 transmembrane proteins (YtrC and YtrD) and a solute-binding protein (YtrF).

224308:BSU 24070
gene id: 224308:BSU_24070
uniprot id: A0A6M4JKJ9
uniprot id: P54532
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the acetokinase family.
messages: Cytoplasm

224308:BSU 30300
gene id: 224308:BSU_30300
uniprot id: A0A6M4JNS0
uniprot id: O34645
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 4 family.
messages: Binds 1 Mn(2+) ion per subunit.
messages: Binds 1 NAD(+) per subunit.
messages: Catalyzes the hydrolysis of melibiose and alpha-galactosides of the raffinose family of oligosaccharides (RFOs) such as raffinose and stachyose. Cannot act on polymeric substrates such as locust bean gum.
messages: Cytoplasm
messages: Homodimer.
messages: Repressed by the transcriptional regulator MelR. Induced by melibiose and raffinose.

224308:BSU 01200
gene id: 224308:BSU_01200
uniprot id: A0A6M4JHS9
uniprot id: P21476
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS19 family.
messages: Part of the 30S ribosomal subunit.
messages: Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.

224308:BSU 03350
gene id: 224308:BSU_03350
uniprot id: C0SP99
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YkuD family.
messages: Cell membrane
messages: Repressed by zinc via the metallo-regulatory protein zur.

224308:BSU 02270
gene id: 224308:BSU_02270
uniprot id: A0A6M4JCC6
uniprot id: P39823
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
messages: Cell membrane
messages: Cells appear normal, no effect on flotillin cluster numbers or size.
messages: Membrane

224308:BSU 33850
gene id: 224308:BSU_33850
uniprot id: A0A6M4JPA9
uniprot id: O32244
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0056 (MarC) family.
messages: Cell membrane
messages: Membrane

224308:BSU 25160
gene id: 224308:BSU_25160
uniprot id: A0A6M3ZDW4
uniprot id: P54473
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the IspH family.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

224308:BSU 19680
gene id: 224308:BSU_19680
uniprot id: A0A6M4JK15
uniprot id: O31864
names: Similarity
messages: Belongs to the UPF0346 family.

224308:BSU 00980
gene id: 224308:BSU_00980
uniprot id: P17869
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Association with RNAP core increases during sporulation but not tested stresses (at protein level).
messages: Belongs to the sigma-70 factor family.
messages: Interacts transiently with the RNAP core.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. This sigma factor is involved in the transition to post-exponential phase in the beginning of sporulation. It is also required for transcription of several stationary phase genes. Association with the RNAP core increases rapidly in early exponential phase, and reamins constant expression level after (PubMed:21710567).

224308:BSU 25010
gene id: 224308:BSU_25010
uniprot id: A0A6M4JJH3
uniprot id: P54487
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38300
gene id: 224308:BSU_38300
uniprot id: A0A6M3ZIF1
uniprot id: P39593
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Thz kinase family.
messages: Binds 2 magnesium ions per subunit. The first is coordinated via water, the second is coordinated to ATP but its significance is unclear.
messages: Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).
messages: Homotrimer.

224308:BSU 21450
gene id: 224308:BSU_21450
uniprot id: O31986
names: Function
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: Transfers a hexose moiety onto 'Cys-41' of bacteriocin sublancin-168 (SunA). Accepts UDP-glucose (UDP-Glc), UDP-N-acetylglucosamine (UDP-GlcNAc), UDP-galactose (UDP-Gal), UDP-xylose (UDP-Xyl) and GDP-mannose as substrate.

224308:BSU 37400
gene id: 224308:BSU_37400
uniprot id: P71008
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in the production of the bacteriocin subtilosin. Required for immunity to subtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 24140
gene id: 224308:BSU_24140
uniprot id: A0A6M3ZDW2
uniprot id: P45858
names: Developmental Stage
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the citrate synthase family.
messages: Bifunctionality as a citrate and 2-methylcitrate synthase is likely important in the mother cell's physiological milieu.
messages: Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
messages: Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Has both 2-methylcitrate synthase and citrate synthase activities. Catalyzes the condensation of propionyl-CoA and oxaloacetate to yield 2-methylcitrate (2-MC) and CoA, and the condensation of acetyl-CoA and oxaloacetate to yield citrate and CoA (PubMed:8759838, PubMed:28956599). Has 2.3-fold higher activity as a 2-methylcitrate synthase (PubMed:28956599). Catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).
messages: Subject to catabolite repression.

224308:BSU 05630
gene id: 224308:BSU_05630
uniprot id: A0A6M3ZB47
uniprot id: Q02886
names: Similarity
names: Subunit
messages: Belongs to the DinB family.
messages: Homodimer.

224308:BSU 23240
gene id: 224308:BSU_23240
uniprot id: P17622
names: Function
messages: Involved in riboflavin biosynthesis.

224308:BSU 20350
gene id: 224308:BSU_20350
uniprot id: A0A6M3ZHZ7
uniprot id: O31903
names: Function
names: Similarity
messages: Belongs to the RecJ family.
messages: Putative single-stranded-DNA-specific exonuclease.

224308:BSU 07900
gene id: 224308:BSU_07900
uniprot id: A0A6M3Z8I7
uniprot id: O34437
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 27060
gene id: 224308:BSU_27060
uniprot id: A0A6M3ZJK6
uniprot id: P26380
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By fructose and LevR.
messages: Cytoplasm
messages: LevD and LevE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator.
messages: The PTS EIIB type-4 domain is phosphorylated by phospho-EIIA on a histidyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-4 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LevDE PTS system is involved in fructose transport.

224308:BSU 30180
gene id: 224308:BSU_30180
uniprot id: P53560
names: Similarity
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.

224308:BSU 11010
gene id: 224308:BSU_11010
uniprot id: O06745
names: Induction
names: Similarity
messages: In the C-terminal section; belongs to the methylenetetrahydrofolate reductase family.
messages: Induced by methionine starvation.

224308:BSU 22500
gene id: 224308:BSU_22500
uniprot id: P42979
names: Subunit
messages: Homodimer, or homotetramer.

224308:BSU 30830
gene id: 224308:BSU_30830
uniprot id: A0A6M3ZG18
uniprot id: P23973
names: Caution
names: Function
names: Similarity
messages: Belongs to the isochorismate synthase family.
messages: Catalyzes the conversion of chorismate to isochorismate.
messages: Used to include what was called 'menR'.

224308:BSU 08380
gene id: 224308:BSU_08380
uniprot id: A0A6M3Z8P3
uniprot id: O31561
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 10570
gene id: 224308:BSU_10570
uniprot id: O07568
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AbrB family.
messages: Cell membrane

224308:BSU 08190
gene id: 224308:BSU_08190
uniprot id: P54717
names: Function
names: Induction
messages: By maltose; repressed by glucose.
messages: Positive regulator of the glv operon expression, which consists of GlvA, GlvR and GlvC.

224308:BSU 12770
gene id: 224308:BSU_12770
uniprot id: P54343
names: Similarity
messages: To B.subtilis YqcE.

224308:BSU 34760
gene id: 224308:BSU_34760
uniprot id: A0A6M3ZIU3
uniprot id: O06974
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the gluconeogenesis factor family.
messages: Cytoplasm
messages: Deletion leads to growth defects when cells are grown on minimal medium containing ribose, gluconate, citrate, fumarate or succinate, but does not affect growth and morphology on minimal medium containing glycolytic substrates such as glucose, sucrose or glycerol. Mutants exhibit media-dependent filamentous or L-shape-like aberrant morphologies.
messages: Required for morphogenesis under gluconeogenic growth conditions.
messages: Required for morphogenesis under gluconeogenic growth conditions. Required, in gluconeogenic growth conditions, for the correct localization of PBP1 and hence for displaying a normal rod shape.
messages: When bacteria are grown on a gluconeogenic carbon source, overproduction can restore a normal rod-shaped morphology in a mreB mutant by rescuing the PBP1 localization.

224308:BSU 39730
gene id: 224308:BSU_39730
uniprot id: A0A6M3ZID5
uniprot id: P42415
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the TPP enzyme family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG).

224308:BSU 07380
gene id: 224308:BSU_07380
uniprot id: O06475
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 13600
gene id: 224308:BSU_13600
uniprot id: A0A6M4JJB1
uniprot id: O31667
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. MtnX family.
messages: Dephosphorylates 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P) yielding 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

224308:BSU 11680
gene id: 224308:BSU_11680
uniprot id: O31617
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the sulfur carrier protein ThiS family.
messages: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by ThiF, then thiocarboxylated (-COSH) by ThiI.
messages: Forms heterodimers with ThiG.
messages: Is the sulfur donor in the synthesis of the thiazole phosphate moiety of thiamine phosphate.

224308:BSU 08100
gene id: 224308:BSU_08100
uniprot id: O31551
names: Function
names: Induction
messages: Acts as a transcriptional activator of the acoABCL operon encoding the acetoin dehydrogenase complex.
messages: Negatively controlled by CcpA, a global regulator of carbon catabolite repression.

224308:BSU 16090
gene id: 224308:BSU_16090
uniprot id: A0A6M4JJZ3
uniprot id: P80886
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the succinate/malate CoA ligase beta subunit family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Heterotetramer of two alpha and two beta subunits (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Heterotetramer of two alpha and two beta subunits.
messages: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.

224308:BSU 03330
gene id: 224308:BSU_03330
uniprot id: A0A6M3ZAF0
uniprot id: P42432
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.
messages: Cell membrane
messages: May function as a nitrate transporter.
messages: Membrane
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 34300
gene id: 224308:BSU_34300
uniprot id: P71057
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance.
messages: Repressed by SinR.

224308:BSU 12720
gene id: 224308:BSU_12720
uniprot id: P54338
names: Similarity
messages: To B.subtilis YqbS.

224308:BSU 34000
gene id: 224308:BSU_34000
uniprot id: A0A6M4JPB5
uniprot id: O32256
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 11690
gene id: 224308:BSU_11690
uniprot id: A0A6M3Z9F8
uniprot id: O31618
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ThiG family.
messages: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.
messages: Cytoplasm
messages: Homotetramer. Forms heterodimers with either ThiH or ThiS.

224308:BSU 05430
gene id: 224308:BSU_05430
uniprot id: A0A6M3Z7Y1
uniprot id: P96687
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.
messages: Cell membrane
messages: Membrane
messages: Regulated by the two-component regulatory system YdfH/YdfI.

224308:BSU 19710
gene id: 224308:BSU_19710
uniprot id: A0A6M3ZCY1
uniprot id: O34984
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M20A family.
messages: Binds 2 Zn(2+) or Co(2+) ions per subunit.

224308:BSU 26140
gene id: 224308:BSU_26140
uniprot id: P45921
names: Function
names: Similarity
messages: Belongs to the encapsulin family. Family 3 subfamily.
messages: Possibly a prophage capsid protein.

224308:BSU 27600
gene id: 224308:BSU_27600
uniprot id: A0A6M3ZJM5
uniprot id: O54408
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Activated by the cyclic di-AMP (c-di-AMP) receptor DarB in the absence of c-di-AMP.
messages: Belongs to the RelA/SpoT family.
messages: Belongs to the relA/spoT family.
messages: Decreased growth rate; growth is almost completely restored in a triple relA-yjbM-ywaC mutant (PubMed:18067544).
messages: Homodimer (PubMed:33619274). At very low potassium concentrations, when intracellular levels of c-di-AMP are low, interacts with apo-DarB (PubMed:33619274). c-di-AMP inhibits the binding of DarB to RelA (PubMed:33619274).
messages: In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.
messages: In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp, it is probably the hydrolysis activity that is required for optimal growth (Probable).

224308:BSU 26580
gene id: 224308:BSU_26580
uniprot id: P39842
names: Function
messages: Activates transcription of the blt gene in response to structurally dissimilar drugs.

224308:BSU 26720
gene id: 224308:BSU_26720
uniprot id: O07920
names: Function
messages: Transcriptional repressor of the azlBCD operon involved in branched-chain amino acid transport.

224308:BSU 25490
gene id: 224308:BSU_25490
uniprot id: A0A6M4JLY1
uniprot id: P25499
names: Function
names: Similarity
messages: Belongs to the HrcA family.
messages: Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.

224308:BSU 36280
gene id: 224308:BSU_36280
uniprot id: P94593
names: Similarity
names: Subunit
messages: Belongs to the SNF2/RAD54 helicase family.
messages: Interacts with the RNA polymerase core.

224308:BSU 09890
gene id: 224308:BSU_09890
uniprot id: C0SPB4
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane

224308:BSU 37380
gene id: 224308:BSU_37380
uniprot id: P71010
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in the production of the bacteriocin subtilosin. Required for maximal production and for optimal immunity to subtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 28370
gene id: 224308:BSU_28370
uniprot id: A0A6M3ZEW3
uniprot id: P28619
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the RNase PH family.
messages: Correct processing of the 3' end of 23S rRNA no longer occurs in the absence of mrnC.
messages: Homohexameric ring arranged as a trimer of dimers (PubMed:14767080). It has been suggested that the active form is the dimer which binds tRNA and that the hexameric form protects the substrate recognition loop (approximately residues 65-82) from proteolysis (PubMed:14767080).
messages: Homohexameric ring arranged as a trimer of dimers.
messages: Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.
messages: Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation. Plays a role in the secondary pathway of 23S rRNA 3' end maturation (PubMed:19880604).
messages: Sulfate ions in the crystal structure may represent the inorganic phosphate substrate.

224308:BSU 30595
gene id: 224308:BSU_30595
uniprot id: C0SP84
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein SsuA/TauA family.
messages: Cell membrane

224308:BSU 17100
gene id: 224308:BSU_17100
uniprot id: A0A6M3ZB17
uniprot id: O34825
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FabD family.
messages: Belongs to the fabD family.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK).

224308:BSU 06812
gene id: 224308:BSU_06812
uniprot id: A0A6M3Z837
uniprot id: O31506
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Deletion of the yeeF-yezG operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Interacts with cognate immunity protein YezG but not with non-cognate immunity protein YobK. The interaction probably inhibits the toxic activity of YeeF.
messages: Membrane
messages: Membrane raft
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YezG does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation (PubMed:34280190). Binds DNA in the presence and absence of YezG (By similarity).
messages: Was originally suggested to be an RNase.

224308:BSU 31875
gene id: 224308:BSU_31875
uniprot id: A0A6M3ZG80
uniprot id: C0SPA7
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EssC family.
messages: Cell membrane
messages: Cells lacking this gene are blocked in YukE secretion (PubMed:23861817, PubMed:24798022). They display an increased bacteriophage SPP1 resistance phenotype (PubMed:15576783).
messages: Membrane
messages: Required for YukE secretion. Probable component or regulator of the ESX/ESAT-6-like secretion system (BsEss).

224308:BSU 10310
gene id: 224308:BSU_10310
uniprot id: O07614
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 00010
gene id: 224308:BSU_00010
uniprot id: P05648
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DnaA family.
messages: Cytoplasm
messages: Interacts (via domains I (1-82) and III (111-326)) with CcrZ (PubMed:35576203). Some 20 DnaA protein molecules bind their sites in oriC.
messages: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes.

224308:BSU 01170
gene id: 224308:BSU_01170
uniprot id: A0A6M3Z6P3
uniprot id: P42921
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL4 family.
messages: Forms part of the polypeptide exit tunnel.
messages: One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.
messages: Part of the 50S ribosomal subunit.

224308:BSU 31740
gene id: 224308:BSU_31740
uniprot id: P14203
names: Caution
messages: Was originally thought to be comB, a competence gene.

224308:BSU 02260
gene id: 224308:BSU_02260
uniprot id: O31452
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AB hydrolase superfamily.
messages: Cytoplasm
messages: Shows carboxylesterase activity in vitro.

224308:BSU 16170
gene id: 224308:BSU_16170
uniprot id: A0A6M4JIM3
uniprot id: P39779
names: Activity Regulation
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activity of CodY is modulated by interaction with two types of effectors: the branched-chain amino acids (BCAAs) leucine, isoleucine and valine, which are signals of the nutritional status of the cell, and GTP, which may signal the energetic status of the cell (PubMed:11331605, PubMed:15228537, PubMed:16488888, PubMed:17993518, PubMed:18083814, PubMed:19749041, PubMed:21764931, PubMed:25645558). During growth in a nutrient-rich medium, the concentrations of these effectors are high, and CodY, once activated by binding to these effectors, shows an increased affinity for its target promoters (PubMed:11331605, PubMed:15228537, PubMed:17993518, PubMed:18083814). GTP and the BCAAs act additively to increase the affinity of CodY for DNA (PubMed:17993518, PubMed:18083814). When nutrients become limiting, the concentrations of BCAAs and GTP drop, leading to dissociation of CodY from the DNA and derepression of genes that are required for adaptation to nutrient-poor conditions (PubMed:11331605, PubMed:15228537, PubMed:17993518). Interacts specifically with GTP and dGTP; no other naturally occurring nucleotides that were tested, including ppGpp and pppGpp, result in DNA protection (PubMed:17993518). GTP hydrolysis is not required for DNA binding (PubMed:17993518).
messages: Belongs to the CodY family.
messages: Cytoplasm
messages: DNA-binding global transcriptional regulator which is involved in the adaptive response to starvation and acts by directly or indirectly controlling the expression of numerous genes in response to nutrient availability. During rapid exponential growth, CodY is highly active and represses genes whose products allow adaptation to nutrient depletion.
messages: Homodimer (PubMed:15937175, PubMed:16488888, PubMed:19500589, PubMed:28011634). Homotetramer (PubMed:28011634). Homodimer under physiological conditions, but homotetramers are observed in the crystals (PubMed:28011634).
messages: In B.subtilis, CodY targets include several hundred genes involved in many different processes, including sporulation, competence, transport systems and catabolic processes (PubMed:12618455, PubMed:23569278). CodY bound to GTP represses many stationary-phase and some early-sporulation genes during growth in excess nutrients (PubMed:11331605). Among others, it binds to the ilvB promoter region and mediates regulation of the ilvB operon by GTP and branched-chain amino acids (BCAAs) (PubMed:15228537, PubMed:17993518, PubMed:18083814, PubMed:19749041). It represses expression of the dipeptide transport operon (dpp) during the exponential growth phase in rich medium (PubMed:7783641, PubMed:11331605, PubMed:18083814). Is also involved in the repression of competence by casamino acids via direct binding and repression of the srfA and comK promoter regions (PubMed:8830686). CodY acts predominantly as a repressor of transcription, but it can also activate transcription of a few genes, including the acetate kinase gene ackA, whose expression is primarily dependent on BCAA levels and only secondarily on GTP levels (PubMed:16995897). Binds to a 15-bp canonical consensus DNA motif, the CodY-binding site (PubMed:18083814, PubMed:21764931, PubMed:23569278). CodY operators may contain multiple CodY-binding sites with 6-bp overlaps (PubMed:21764931). Most CodY-binding sites are in intergenic regions, but sites located within coding regions were also identified (PubMed:23569278).
messages: Modular protein made up of an N-terminal effector binding GAF-type domain and a C-terminal wHTH DNA binding domain, separated by a helical linker (PubMed:16488888, PubMed:28011634). The GAF domain is involved in the formation of homodimers (PubMed:28011634). Amino acid binding to the GAF domain is associated with drastic but localized changes in structure (PubMed:19500589). Structural changes upon isoleucine binding may lead to increased affinity of Ile-bound CodY for its target DNA (PubMed:28011634).

224308:BSU 23940
gene id: 224308:BSU_23940
uniprot id: P54539
names: Similarity
messages: Belongs to the YkuD family.

224308:BSU 38840
gene id: 224308:BSU_38840
uniprot id: A0A6M3ZHY7
uniprot id: P94357
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 23250
gene id: 224308:BSU_23250
uniprot id: A0A6M3ZDG1
uniprot id: P11998
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DMRL synthase family.
messages: Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
messages: Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. Is able to use the non-natural R enantiomer of 3,4-dihydroxy-2-butanone 4-phosphate as a substrate, but with less efficiency than the natural S enantiomer. Cannot use unphosphorylated 3,4-dihydroxy-2-butanone, 3,4-dihydroxy-2-butanone 3-phosphate or diacetyl as substrates.
messages: Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.
messages: Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers. Can interact with riboflavin synthase, forming a lumazine synthase/riboflavin synthase complex, also designated as 'heavy riboflavin synthase complex', which consists of a trimer of riboflavin synthase enclosed within the icosahedral structure composed of 60 subunits of 6,7-dimethyl-8-ribityllumazine synthase.

224308:BSU 08030
gene id: 224308:BSU_08030
uniprot id: A0A6M4JGH1
uniprot id: O31546
names: Function
names: Similarity
messages: Belongs to the Dus family.
messages: Belongs to the dus family.
messages: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.

224308:BSU 35240
gene id: 224308:BSU_35240
uniprot id: A0A6M3ZGF4
uniprot id: O35002
names: Activity Regulation
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activated by peptide binding to the PDZ domain.
messages: Belongs to the peptidase S41A family.
messages: Forespore intermembrane space
messages: Homodimer.
messages: Involved in the signal transduction pathway leading to the proteolytic activation of the mother cell transcription factor pro-sigma-K during sporulation. The signaling serine protease CtpB triggers pro-sigma-K processing by cleaving the pre-processed regulatory protein SpoIVFA and is necessary for the proper timing of sigma-K activation.
messages: Is cleaved by SpoIVB in vitro and in vivo but this cleavage does not appear to be necessary for CtpB activation. CtpB can also cleave itself in vivo.
messages: Is expressed in the forespore under the control of sigma-G, and in the mother cell under the control of sigma-E.
messages: Pro-sigma-K processing is delayed by approximately 30 minutes, and sporulation efficiency is reduced approximately two-fold.
messages: The PDZ domain functions as a gatekeeper to the protease tunnel and defines resting and active conformations of the protease.

224308:BSU 12340
gene id: 224308:BSU_12340
uniprot id: A0A6M3ZA09
uniprot id: O34346
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the mannonate dehydratase family.
messages: Catalyzes the dehydration of D-mannonate.
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 07150
gene id: 224308:BSU_07150
uniprot id: O31534
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
messages: Belongs to the antibiotic biosynthesis monooxygenase family.
messages: Cytoplasm
messages: Homodimer.
messages: Neither hmoA single mutant nor the hmoA hmoB double mutant display robust and reproducible phenotypes relative to the wild-type.
messages: Repressed by fur in the presence of iron.

224308:BSU 38070
gene id: 224308:BSU_38070
uniprot id: P26212
names: Function
names: PTM
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Mediates positive regulation of the sacPA operon by functioning as an antiterminator factor of transcription.
messages: Phosphorylated and inactivated by SacP (EII-Scr).

224308:BSU 38490
gene id: 224308:BSU_38490
uniprot id: A0A6M4JQJ8
uniprot id: P39582
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MenA family. Type 1 subfamily.
messages: Cell membrane
messages: Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to demethylmenaquinone (DMK).
messages: Membrane

224308:BSU 33960
gene id: 224308:BSU_33960
uniprot id: A0A6M3ZGR6
uniprot id: P96710
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Deletion of the gene does not affect growth on glucose, but the mutant cannot grow in the presence of arabinose. Deletion has a negative effect on the ability of the mutant to grow on alpha-1,5-arabinobiose. AraE/araN double mutant is unable to grow in the presence of alpha-1,5-arabinobiose.
messages: Membrane
messages: Transcription is repressed by the binding of AraR to the promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.
messages: Uptake of L-arabinose across the cytoplasmic membrane with the concomitant transport of protons into the cell (symport system) (PubMed:9401028). In the presence of inducing amounts of L-arabinose, can import both D-galactose and D-xylose (PubMed:9620981). Can also transport the disaccharide alpha-1,5-arabinobiose (PubMed:20693325).

224308:BSU 37320
gene id: 224308:BSU_37320
uniprot id: A0A6M3ZI50
uniprot id: P46907
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.
messages: Cell membrane
messages: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate.
messages: Membrane

224308:BSU 12980
gene id: 224308:BSU_12980
uniprot id: A0A6M3ZIP7
uniprot id: O34508
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the mandelate racemase/muconate lactonizing enzyme family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L-Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipeptides L-Ala-L-Asp, L-Ala-L-Leu, L-Ala-L-Met, and L-Ala-L-Ser. Is not able to epimerize other L-Ala-X dipeptides. Is also active with L-Ser-L-Glu and, oddly, L-Pro-L-Glu, but not with L-Glu-L-Glu, L-Lys-L-Glu, L-Lys-L-Ala, or D-Ala-D-Ala.
messages: Homooctamer; tetramer of dimers.
messages: Repressed by AbrB, a transcription factor that negatively controls biofilm formation.

224308:BSU 35440
gene id: 224308:BSU_35440
uniprot id: P39807
names: Function
names: Similarity
messages: May be involved in the assembly, structure, or function of the flagellum. May polymerize to form a filamentous structure that is part of the flagellum.
messages: To S.typhimurium FliF.

224308:BSU 20930
gene id: 224308:BSU_20930
uniprot id: O31934
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0715 family.
messages: Cell membrane

224308:BSU 11990
gene id: 224308:BSU_11990
uniprot id: O31643
names: Induction
messages: Expression is induced in response to phosphate starvation in a PhoR-dependent manner.

224308:BSU 13590
gene id: 224308:BSU_13590
uniprot id: A0A6M4JHH2
uniprot id: O31666
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the RuBisCO large chain family. Type IV subfamily.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P).
messages: Has no RuBP-carboxylation activity.
messages: Homodimer.

224308:BSU 34110
gene id: 224308:BSU_34110
uniprot id: O07014
names: Domain
names: Function
names: Induction
messages: Induced by entry into stationary phase, but not by carbon and energy starvation.
messages: Positive regulator of sigma-B activity. Dephosphorylates RsbV in response to energy stress.
messages: The PAS domain may participate in sensing the overall energy level of the cell and communicate this information to the phosphatase domain.

224308:BSU 25810
gene id: 224308:BSU_25810
uniprot id: P45949
names: Function
messages: Transcriptional repressor for the ars operon.

224308:BSU 27080
gene id: 224308:BSU_27080
uniprot id: P23914
names: Domain
names: Function
names: PTM
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Involved in positive regulation of the levanase operon which comprises the levDEFG genes for a fructose PTS system, and sacA for levanase.
messages: Possibly phosphorylated and inactivated by the PTS system.
messages: The PTS EIIA type-4 domain may serve a regulatory function, through its phosphorylation activity.

224308:BSU 07630
gene id: 224308:BSU_07630
uniprot id: A0A6M4JG05
uniprot id: O34453
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the NOS family. Bacterial NOS oxygenase subfamily.
messages: Catalyzes the production of nitric oxide.
messages: Homodimer.
messages: Tetrahydrobiopterin (H4B). Can also use tetrahydrofolate (THF) in place of tetrahydrobiopterin (H4B) but binds THF with much lower affinity than H4B.
messages: This protein is similar to the oxygenase domain of eukaryotic nitric oxide synthases but lacks the reductase domain which, in eukaryotes, is responsible for transfer of electrons to the ferric heme during nitric oxide synthesis.

224308:BSU 28060
gene id: 224308:BSU_28060
uniprot id: P37575
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in endospore development.
messages: Under sporulation control.

224308:BSU 15920
gene id: 224308:BSU_15920
uniprot id: A0A6M4JLT2
uniprot id: P80643
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.
messages: 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.
messages: Belongs to the acyl carrier protein (ACP) family.
messages: Carrier of the growing fatty acid chain in fatty acid biosynthesis.
messages: Cytoplasm

224308:BSU 01845
gene id: 224308:BSU_01845
uniprot id: A0A6M4JCE4
uniprot id: O34688
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the inorganic carbon transporter (TC 9.A.2) DabA family.
messages: Cell membrane
messages: Forms a complex with DabB.
messages: Part of an energy-coupled inorganic carbon pump.

224308:BSU 27990
gene id: 224308:BSU_27990
uniprot id: A0A6M3ZHZ9
uniprot id: Q01464
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings (By similarity).
messages: ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.
messages: Belongs to the ParA family. MinD subfamily.
messages: Cell membrane
messages: Interacts with MinC and FtsZ (By similarity). Interacts with MinJ.

224308:BSU 15220
gene id: 224308:BSU_15220
uniprot id: A0A6M4JG48
uniprot id: P37585
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 28 family. MurG subfamily.
messages: Cell membrane
messages: Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 08090
gene id: 224308:BSU_08090
uniprot id: A0A6M3Z8G0
uniprot id: O34324
names: Cofactor
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
messages: Binds 1 FAD per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: The active site is a redox-active disulfide bond.

224308:BSU 33980
gene id: 224308:BSU_33980
uniprot id: A0A6M3ZGG8
uniprot id: O32254
names: Similarity
messages: To bacterial alkanal monooxygenase alpha and beta chains.

224308:BSU 32710
gene id: 224308:BSU_32710
uniprot id: A0A6M3ZFU3
uniprot id: P80866
names: Similarity
messages: Belongs to the ABC transporter superfamily. Ycf16 family.

224308:BSU 05380
gene id: 224308:BSU_05380
uniprot id: P96682
names: Similarity
messages: Belongs to the flavoredoxin family.

224308:BSU 19480
gene id: 224308:BSU_19480
uniprot id: A0A6M3ZBM4
uniprot id: O31859
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane
messages: Membrane

224308:BSU 22060
gene id: 224308:BSU_22060
uniprot id: A0A6M4JIK2
uniprot id: P42086
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
messages: Cell membrane
messages: Membrane
messages: Transport of xanthine in the cell.

224308:BSU 20940
gene id: 224308:BSU_20940
uniprot id: O31935
names: Function
names: Similarity
names: Subunit
messages: Forms a complex with cognate antitoxin YopB.
messages: In the C-terminal section; belongs to the MbcT/ParT/Res family.
messages: May be the toxic component of a type II toxin-antitoxin (TA) system. Neutralized by its cognate antitoxin YopB.

224308:BSU 22700
gene id: 224308:BSU_22700
uniprot id: A3F3D3
uniprot id: P31102
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.
messages: Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).
messages: Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 29630
gene id: 224308:BSU_29630
uniprot id: O34970
names: Induction
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.

224308:BSU 16340
gene id: 224308:BSU_16340
uniprot id: P35536
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: May be a structural component of the flagellum that anchors the rod to the membrane.

224308:BSU 01970
gene id: 224308:BSU_01970
uniprot id: O31429
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Required for production of the bacteriocin SkfA.
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 12210
gene id: 224308:BSU_12210
uniprot id: A0A6M3Z9L6
uniprot id: O34374
names: Similarity
messages: Belongs to the cytochrome P450 family.

224308:BSU 33090
gene id: 224308:BSU_33090
uniprot id: A0A6M4JP28
uniprot id: O32198
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Induced, via LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.
messages: Member of the two-component regulatory system LiaS/LiaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also involved in response to cationic antimicrobial peptides and secretion stress. Activates probably LiaR by phosphorylation.
messages: Membrane

224308:BSU 25040
gene id: 224308:BSU_25040
uniprot id: P54485
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38960
gene id: 224308:BSU_38960
uniprot id: P42318
names: Similarity
messages: To B.subtilis YxjH.

224308:BSU 37570
gene id: 224308:BSU_37570
uniprot id: A0A6M4JQB7
uniprot id: Q00538
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Membrane
messages: Resistance to the epoxide antibiotic methylenomycin.

224308:BSU 19510
gene id: 224308:BSU_19510
uniprot id: O31861
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 11230
gene id: 224308:BSU_11230
uniprot id: A0A6M3Z9S5
uniprot id: P36838
names: Similarity
names: Subunit
messages: Belongs to the CarA family.
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

224308:BSU 17420
gene id: 224308:BSU_17420
uniprot id: A0A6M3ZII8
uniprot id: P27643
names: Developmental Stage
names: Similarity
messages: Belongs to the CbxX/CfxQ family.
messages: Mutations in spoVJ block sporulation at a late stage. Probably only functions late in development.

224308:BSU 37290
gene id: 224308:BSU_37290
uniprot id: P46910
names: Function
names: Induction
messages: Activates, in anaerobic conditions, the transcription of the fermentative operons lctEP and alsDS, of the hmp gene encoding a flavohemoglobin-like protein, the nitrite reductase operon nasDE and the heme biosynthesis genes hemN and hemZ.
messages: By fnr. Autorepressed.

224308:BSU 17930
gene id: 224308:BSU_17930
uniprot id: P45706
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DsbD family.
messages: Cell membrane
messages: Cytochrome c oxidase activity is partially restored by mutations in bdbC or bdbD that are defective in the synthesis of disulfide bond containing proteins. The same mutations abrogate the need for CcdA in sporulation.
messages: Required for cytochrome c synthesis and stage V of sporulation. Might transfer reducing equivalents across the cytoplasmic membrane, promoting efficient disulfide bond isomerization of proteins localized on the outer surface of the membrane or in the spore coat.

224308:BSU 40510
gene id: 224308:BSU_40510
uniprot id: A0A6M4JR32
uniprot id: P37484
names: Activity Regulation
names: Cofactor
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: ATPase activity (residues 84-303) prefers Mg(2+) over Mn(2+).
messages: Belongs to the GdpP/PdeA phosphodiesterase family.
messages: Binds 1 heme (probably heme b) per subunit, probably hexa-coordinated, which inhibits PDE and ATPase. The Fe(2+) binds CN(-), CO and NO.
messages: Cell membrane
messages: Expressed at low levels at the onset of sporulation, it rises during sporulation (at protein level).
messages: For phosphodiesterase activity (residues 84-695), probably binds 2 Mn(2+) per subunit, can also use Co(2+), Mg(2+) or Ni(2+).
messages: For phosphodiesterase activity, probably binds 2 Mn(2+) per subunit.
messages: Has a GGDEF domain (residues 173-310) preceded by a PAS-like domain (residues 84-149) which together have ATPase activity, followed by a region with a DHH (339-496) and a DHHA1 (591-646) domain (PubMed:19901023). The combined GGDEF, DHH and DHHA1 domains have c-di-AMP phosphodiesterase activity (residues 150-659) that is 10-fold enhanced by inclusion of the PAS-like domain (84-149); PDE activity may only require the DHH/DHHA1 domains (PubMed:19901023). The PAS-like domain is important for heme b-binding (PubMed:21257773).
messages: Has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP) and to a much lesser extent against cyclic-di-GMP (c-di-GMP) in the DHH/DHHA1 domains (PubMed:19901023, PubMed:21257773). Also has ATPase activity, probably via the GGDEF domain (PubMed:19901023, PubMed:21257773). Overexpression leads to increased sensitivity to methyl methanesulfonate (MMS) and H(2)O(2) (PubMed:25616256). Overexpression leads to extreme sensitivity to the beta-lactam antibiotic cefuroxime (CEF), probably dependent on PDE activity (PubMed:22211522). May monitor cellular heme or NO levels (PubMed:21257773). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).
messages: Has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP).
messages: Increased resistance to acid stress, increased sporulation efficiency following DNA damage (by nalidixic acid) (PubMed:19901023). Increases c-di-AMP levels in mid-exponential phase from about 3.8 uM to about 4.3 uM in strain BG214 (PubMed:25616256). Increased levels of c-di-AMP 4-fold during sporulation (PubMed:21566650). Insertion mutations in this gene partially restore antibiotic resistance to the beta-lactam antibiotic cefuroxime (CEF) in a sigM deletion mutant (PubMed:22211522). Decreased sensitivity to MMS and H(2)O(2) (PubMed:25616256). In strain 168 grown in minimal medium and glutamate, 2.2-fold increase in c-di-AMP levels while a double pgpH-gdpP mutant has 4.2-fold increased levels of in c-di-AMP; double mutants die on solid medium after 2 days (PubMed:26240071).
messages: Phosphodiesterase (PDE) inhibited by Zn(2+), Ca(2+) inhibits in the presence of Mg(2+) but not Mn(2+); c-di-AMP PDE activity is competitively inhibited by ppGpp (PubMed:19901023). Heme binding (by Fe(2+) or Fe(3+) heme) inhibits PDE, activity is partially restored by KCN or NO only for Fe(2+) heme (PubMed:21257773). Binding of NO to Fe(2+) heme switches from hexa- to pentacoordination (PubMed:21257773). Heme binding inhibits the ATPase activity (PubMed:21257773).
messages: Probably transcribed under control of the sigma A factor (sigA), protein levels are negatively regulated by an antisense RNA (PubMed:22956758).

224308:BSU 04170
gene id: 224308:BSU_04170
uniprot id: P96575
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.

224308:BSU 10320
gene id: 224308:BSU_10320
uniprot id: O07615
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
messages: Cytoplasm
messages: Homodimer, or homotetramer.

224308:BSU 24520
gene id: 224308:BSU_24520
uniprot id: A0A6M4JJ36
uniprot id: P54512
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DtxR/MntR family.
messages: Central regulator of manganese homeostasis that regulates the expression of both manganese uptake and efflux systems (PubMed:27748968, PubMed:10760146, PubMed:12950915). In the presence of high levels of manganese, it mediates repression of the manganese uptake systems MntH and MntABCD and activation of the efflux systems MneP and MneS. Binds with high affinity to the regulatory regions of its target genes (PubMed:27748968, PubMed:12950915). The manganese concentration required for activation of efflux is higher than that for repression of uptake (PubMed:27748968).
messages: Central regulator of manganese homeostasis.
messages: Contains an N-terminal DNA-binding domain and a C-terminal dimerization domain (PubMed:12847518, PubMed:16533030, PubMed:23298157). Contains two metal binding sites per subunit, site A (corresponding to metal ion 2) and site C (corresponding to metal ion 1), which both contribute to the metal selectivity of MntR. A large metal cation is needed at the A site to correctly orient and position the C site ligands. Smaller, non-cognate metal cations bind at the A site, but disrupt the C site, blocking the full activation of MntR. Binding at the C site also favors Mn(2+) and Cd(2+) over other metals (PubMed:16533030, PubMed:23298157).
messages: Cytoplasm
messages: DNA binding is strongly activated by Mn(2+) and Cd(2+), but it is poorly activated by non-cognate metal cations, including Co(2+), Fe(2+), Ni(2+), Ca(2+) and Zn(2+). In the strict absence of divalent transition metal ions, MntR has a low affinity for DNA.
messages: DNA binding is strongly activated by Mn(2+).
messages: Homodimer.
messages: Null mutant is very sensitive to manganese (PubMed:27748968, PubMed:10760146). Mutant also displays increased sensitivity to cadmium (PubMed:10760146).
messages: Was originally thought to act as an activator for the mntABCD operon.

224308:BSU 06000
gene id: 224308:BSU_06000
uniprot id: O05524
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38340
gene id: 224308:BSU_38340
uniprot id: A0A6M4JQG2
uniprot id: P39589
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 25680
gene id: 224308:BSU_25680
uniprot id: P54452
names: Disruption Phenotype
names: Function
messages: Has low dephosphorylation activity on GMP and glucose-6-phosphate.
messages: Required for growth on solid medium.

224308:BSU 40250
gene id: 224308:BSU_40250
uniprot id: A0A6M4JQY0
uniprot id: Q45604
names: Disruption Phenotype
names: Similarity
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Cells lacking this gene retain acetoin reductase/2,3-butanediol dehydrogenase activity.

224308:BSU 11240
gene id: 224308:BSU_11240
uniprot id: A0A6M3Z9L3
uniprot id: P18185
names: Caution
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the CarB family.
messages: Binds 4 Mg(2+) or Mn(2+) ions per subunit.
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 32530
gene id: 224308:BSU_32530
uniprot id: O32149
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M20 family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: Involved in the anaerobic nitrogen utilization via the assimilation of allantoin (PubMed:11344136). Catalyzes specifically the hydrolysis of allantoate to yield CO2, NH3 and S-ureidoglycine, which is unstable and readily undergoes a second deamination by S-ureidoglycine aminohydrolase AllE to yield S-ureidoglycolate and NH3 (By similarity).
messages: Transcriptionally regulated by PucR. Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin is added during limiting-nitrogen conditions.

224308:BSU 00170
gene id: 224308:BSU_00170
uniprot id: P37532
names: Similarity
messages: Belongs to the isochorismatase family.

224308:BSU 33810
gene id: 224308:BSU_33810
uniprot id: O32243
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the OsmX family.
messages: Cell membrane
messages: Member of a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine, and choline.
messages: Repressed by OpcR.
messages: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).

224308:BSU 16770
gene id: 224308:BSU_16770
uniprot id: A0A6M4JIC1
uniprot id: Q04796
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DapA family.
messages: Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).
messages: Cytoplasm
messages: Homotetramer; dimer of dimers.
messages: Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

224308:BSU 31970
gene id: 224308:BSU_31970
uniprot id: A0A6M4JP03
uniprot id: P45743
names: Cofactor
names: Induction
names: Similarity
messages: Belongs to the isochorismatase family.
messages: Binds 1 phosphopantetheine covalently.
messages: By superoxide.

224308:BSU 14470
gene id: 224308:BSU_14470
uniprot id: A0A6M3ZA92
uniprot id: P39763
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis has three MreB paralogs: MreB, Mbl and MreBH. All paralogs have the ability to support rod-shaped cell growth normal conditions. The multiplicity of paralogs becomes important under stress conditions. They are probably used to allow cells to maintain proper growth and morphogenesis under changing conditions.
messages: Belongs to the FtsA/MreB family.
messages: Cytoplasm
messages: Disruption of the gene leads to cells that are curved, bent at irregular angles and affected in length and width.
messages: Expression is sigma I-dependent.
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination (PubMed:16950129, PubMed:19659933). Acts by regulating cell wall synthesis and cell elongation, and thus cell shape (PubMed:16950129, PubMed:19659933). A feedback loop between cell geometry and MreBH localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature (By similarity). Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreBH polymerization (PubMed:21636745, PubMed:21636744). Organizes peptidoglycan synthesis in the lateral cell wall (PubMed:19659933). Required for the localization of the cell wall hydrolase LytE into the cylindrical part of the cell wall (PubMed:16950129).
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.
messages: Forms polymers (PubMed:16950129). Forms a complex with Mbl and MreB (PubMed:19659933). Interacts with the C-terminal catalytic domain of LytE (PubMed:16950129).
messages: Forms polymers.
messages: Membrane raft

224308:BSU 12290
gene id: 224308:BSU_12290
uniprot id: P80861
names: Induction
names: Similarity
messages: Belongs to the NADH dehydrogenase family.
messages: By glucose starvation.

224308:BSU 03380
gene id: 224308:BSU_03380
uniprot id: A0A6M4JCP7
uniprot id: P42400
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: Probably part of a binding-protein-dependent transport system.

224308:BSU 24460
gene id: 224308:BSU_24460
uniprot id: P54518
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M24B family.
messages: Binds 2 manganese ions per subunit.

224308:BSU 02490
gene id: 224308:BSU_02490
uniprot id: A0A6M3Z6X8
uniprot id: P42238
names: Function
names: Similarity
messages: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily.
messages: Catalyzes the dehydration of glucarate to 5-keto-4-deoxy-D-glucarate (5-kdGluc).

224308:BSU 01310
gene id: 224308:BSU_01310
uniprot id: A0A6M4JC43
uniprot id: P46898
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL6 family.
messages: Part of the 50S ribosomal subunit.
messages: This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.

224308:BSU 17750
gene id: 224308:BSU_17750
uniprot id: A0A6M3ZEP8
uniprot id: O31808
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Membrane

224308:BSU 30340
gene id: 224308:BSU_30340
uniprot id: O34627
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Cells lacking this gene have a twofold decreased response to salt and ethanol stress, and a somewhat reduced response to energy stress, indicating that PhoT is a positive regulator of sigma-B activity. It acts independently of both RsbRA and RsbRB (YkoB).
messages: Exhibits the same spectroscopical features and blue-light induced photochemistry as plants phototropins, with the reversible formation of a blue-shifted photoproduct, assigned to an FMN-cysteine thiol adduct. Although it is a positive regulator in the activation of the environmental signaling branch of the general stress transcription factor sigma-B, its precise role is undetermined.
messages: FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark.
messages: Homodimer.

224308:BSU 24930
gene id: 224308:BSU_24930
uniprot id: O32022
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30270
gene id: 224308:BSU_30270
uniprot id: O34335
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Deletion of the gene abolishes induction in the presence of melibiose and raffinose.
messages: Part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import. Binds melibiose, raffinose and stachyose.
messages: Repressed by the transcriptional regulator MelR. Induced by melibiose and raffinose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MelC and MelD) and a solute-binding protein (MelE).

224308:BSU 11570
gene id: 224308:BSU_11570
uniprot id: A0A6M3ZIK5
uniprot id: O31608
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transglycosylase Slt family.
messages: Exhibits both muramidase and lytic transglycosidase activities (PubMed:20609359). Cleaves the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine (MurNAc-GlcNAc) in peptidoglycan, producing both N-acetylmuramic acid and 1,6-anhydro-N-acetylmuramic acid (PubMed:20609359). Is conditionally required for swarming motility and may play a role in flagella function specifically on harder surface environments, but is not required for flagellar assembly (PubMed:33649146).
messages: Expressed by the motility sigma factor SigD.
messages: Mutants exhibit wild-type swimming motility in liquid, but they exhibit a severe defect in flagellum-dependent swarming motility (PubMed:33649146). Mutants are not defective in flagellar assembly (PubMed:33649146). They have a slight but statistically significant reduction in the number of flagellar hooks and basal bodies (PubMed:33649146).
messages: Secreted
messages: The N-terminal domain is important for secretion and protein stability.

224308:BSU 01730
gene id: 224308:BSU_01730
uniprot id: A0A6M3Z6T3
uniprot id: Q45585
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Alteration (reduction or loss for the most part) of expression of about 60 genes that are high-pH-inducible, including sigW and its anti-sigma factor rsiW (PubMed:11454200).
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: By different stresses causing damage to the cell envelope, such as alkaline shock (PubMed:11454200), salt shock (PubMed:11544224), phage infection and certain antibiotics that affect cell wall biosynthesis (PubMed:12207695, PubMed:15870467). Does not respond to oxidative stress caused by diamide (PubMed:21685450). Association with RNAP core increases during most stresses but not during sporulation (at protein level) (PubMed:21710567).
messages: Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor (RsiW for this protein) until released by regulated membrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal (envelope stress) triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The anti-sigma factor RsiW is a membrane protein, binding sigma-W in the cytoplasm. RsiW is first cut extracytoplasmically (site-1 protease, S1P, by PrsW) (PubMed:16816000), then within the membrane itself (site-2 protease, S2P, by RasP) (PubMed:15130127), while cytoplasmic proteases (predominantly ClpX-ClpP) finish degrading the regulatory protein, liberating sigma-W (PubMed:16899079).
messages: Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription. Forms a heterodimer with cognate anti-sigma factor RsiW, which prevents it from binding to the -10 and -35 promoter elements (PubMed:28319136).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Sigma-W controls genes involved in response to cell envelope stress such as antimicrobial peptides (PubMed:12207695, PubMed:15870467), alkaline pH (PubMed:11454200), transport processes and detoxification.
messages: The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA (PubMed:28319136), and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. Also mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
messages: The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA (PubMed:28319136). The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).

224308:BSU 40150
gene id: 224308:BSU_40150
uniprot id: Q45593
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Suggested to be part of an ABC transporter complex YydIJ involved in export of the modified peptide YydF (Probable). Responsible for energy coupling to the transport system (By similarity).
messages: The complex is composed of two ATP-binding proteins (YydI), two transmembrane proteins (YydJ).
messages: Transcriptionally repressed by rok (PubMed:15743949), this was not found to be the case in another study (PubMed:17921301).
messages: Up-regulates expression of the LiaRS two-component regulatory system; this effect is more pronounced on modified competence medium than on rich or sporulation medium.

224308:BSU 26150
gene id: 224308:BSU_26150
uniprot id: P45920
names: Similarity
messages: To B.subtilis XkdF.

224308:BSU 12930
gene id: 224308:BSU_12930
uniprot id: A0A6M3ZA25
uniprot id: P26903
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Expressed early during sporulation.
messages: Membrane
messages: Nutrient deficiency conditions, which also induce sporulation.
messages: Probably part of the ABC transporter DppBCDE involved in dipeptide transport (Probable). Responsible for the translocation of the substrate across the membrane (Probable).

224308:BSU 10270
gene id: 224308:BSU_10270
uniprot id: O07610
names: Function
names: Induction
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to FadR boxes on target DNA and thus derepress transcription.

224308:BSU 10900
gene id: 224308:BSU_10900
uniprot id: O06734
names: Similarity
messages: Belongs to the AB hydrolase superfamily.

224308:BSU 32470
gene id: 224308:BSU_32470
uniprot id: O32143
names: Cofactor
names: Function
names: Induction
names: Subunit
messages: Binds 2 [2Fe-2S] clusters.
messages: Could be composed of four subunits: PucA, PucC, PucD and PucE.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
messages: Oxidizes hypoxanthine and xanthine to uric acid.

224308:BSU 33590
gene id: 224308:BSU_33590
uniprot id: A0A6M4JP76
uniprot id: O32229
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 33720
gene id: 224308:BSU_33720
uniprot id: A0A6M3ZH04
uniprot id: Q45461
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for choline; probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Repressed by GbsR.

224308:BSU 33920
gene id: 224308:BSU_33920
uniprot id: A0A6M4JPA8
uniprot id: P27876
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the triosephosphate isomerase family.
messages: Cytoplasm
messages: Homodimer.
messages: Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).

224308:BSU 07420
gene id: 224308:BSU_07420
uniprot id: O34512
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 23990
gene id: 224308:BSU_23990
uniprot id: P54534
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the bacilliredoxin family.
messages: Expression is up-regulated by cumene hydroperoxide (CHP).
messages: Interacts with BrxC.
messages: N-terminal Cys of the CXC active site motif can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation protects Cys residues against overoxidation by acting as a redox switch in response to oxidative stress.
messages: S-bacillithiolation is the formation of mixed disulfide bonds between protein thiols and the general thiol reductant bacillithiol (BSH) under oxidative stress. BSH is an equivalent of glutathione (GSH) in Firmicutes. This protein is a dithiol bacilliredoxin, which debacillithiolates (removes BSH) the S-bacillithiolated OhrR (OhrR-SSB) in vitro and in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Involved in maintaining redox homeostasis in response to disulfide stress conditions.
messages: Sensitive to cumene hydroperoxide (CHP) as indicated by growth inhibition assay. No effect on growth after NaOCl treatment.

224308:BSU 37670
gene id: 224308:BSU_37670
uniprot id: A0A6M3ZHI1
uniprot id: P39645
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ChdC family. Type 1 subfamily.
messages: Fe-coproporphyrin III acts as both substrate and redox cofactor.
messages: Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:25646457). Catalyzes the decarboxylation of Fe-coproporphyrin III (coproheme) to heme b (protoheme IX), the last step of the pathway (By similarity). The reaction occurs in a stepwise manner with a three-propionate harderoheme intermediate (By similarity).

224308:BSU 13620
gene id: 224308:BSU_13620
uniprot id: A0A6M3ZFC4
uniprot id: O31669
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the acireductone dioxygenase (ARD) family.
messages: Binds 1 Fe(2+) cation per monomer.
messages: Binds 1 nickel ion per monomer.
messages: Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
messages: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
messages: Monomer.

224308:BSU 26530
gene id: 224308:BSU_26530
uniprot id: A0A6M3ZEJ2
uniprot id: P54433
names: Similarity
messages: Belongs to the sulfur carrier protein TusA family.

224308:BSU 32500
gene id: 224308:BSU_32500
uniprot id: O32146
names: Function
names: Induction
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
messages: Required for xanthine dehydrogenase activity. Could be involved in formation of the molybdenum cofactor required by xanthine dehydrogenase.

224308:BSU 16330
gene id: 224308:BSU_16330
uniprot id: P24072
names: Cofactor
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Binds 1 Mg(2+) ion per subunit.
messages: Cytoplasm
messages: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. Phosphorylated CheY interacts with the flagella switch components FliM and FliY, which causes counterclockwise rotation of the flagella, resulting in smooth swimming.
messages: Phosphorylated CheY binds to FliM and FliY.
messages: Phosphorylated by CheA. Dephosphorylated (inactivated) by FliY and CheC.
messages: The signaling mechanism of chemotaxis in B.subtilis appears to be inverted in comparison to E.coli.

224308:BSU 39770
gene id: 224308:BSU_39770
uniprot id: P46337
names: Function
messages: Iol operon repressor.

224308:BSU 13670
gene id: 224308:BSU_13670
uniprot id: O31672
names: Function
names: Induction
messages: Negatively regulates mhqA, mhqED, mhqNOP, and azoR2 which may contribute to the degradation of aromatic compounds.
messages: Repressed by 2-methylhydroquinone (2-MHQ), diamide and catechol stress. Not subject to autorepression.

224308:BSU 03110
gene id: 224308:BSU_03110
uniprot id: A0A6M4JEZ4
uniprot id: P94383
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 19770
gene id: 224308:BSU_19770
uniprot id: P42091
names: Function
messages: May be involved in maturation of the outermost layer of the spore.

224308:BSU 34890
gene id: 224308:BSU_34890
uniprot id: A0A6M3ZL17
uniprot id: O34565
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Heterodimer of HisH and HisF.
messages: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF (By similarity).
messages: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.

224308:BSU 29340
gene id: 224308:BSU_29340
uniprot id: A0A6M4JKS1
uniprot id: O34900
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. L-cystine importer (TC 3.A.1.3.13) family.
messages: Cell membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Responsible for energy coupling to the transport system (Probable). Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.
messages: The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).

224308:BSU 18830
gene id: 224308:BSU_18830
uniprot id: O34309
names: Caution
names: Function
names: Similarity
messages: Belongs to the PEP-utilizing enzyme family.
messages: Lacks almost all of the known catalytic and substrate-binding residues for this enzyme; hence it is annotated as putative.
messages: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate.

224308:BSU 18920
gene id: 224308:BSU_18920
uniprot id: O31840
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Phr family.
messages: CSF, PhrF and PhrK stimulate ComA-dependent gene expression to different levels and are all required for full expression of genes activated by ComA.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably only subject to processing events at the ends of the mature peptide.
messages: Cytoplasm
messages: Deletion of the gene results in decreased expression of genes activated by ComA, and significantly changes the expression of 40 operons.
messages: Secreted
messages: Signaling molecule involved in the regulation of genetic competence development (PubMed:16816200). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (By similarity). Stimulates expression of the genes controlled by ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:16816200). Acts by inhibiting RapK, which regulates the activity of ComA (PubMed:16816200).

224308:BSU 17010
gene id: 224308:BSU_17010
uniprot id: O31778
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the methylthiotransferase family. MiaB subfamily.
messages: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
messages: Cytoplasm
messages: Monomer.

224308:BSU 09000
gene id: 224308:BSU_09000
uniprot id: O31593
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.
messages: Cell membrane

224308:BSU 37010
gene id: 224308:BSU_37010
uniprot id: A0A6M4JRN8
uniprot id: P45872
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the prokaryotic/mitochondrial release factor family.
messages: Cytoplasm
messages: Methylated by PrmC. Methylation increases the termination efficiency of RF1 (By similarity).
messages: Methylated by PrmC. Methylation increases the termination efficiency of RF1.
messages: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.

224308:BSU 05850
gene id: 224308:BSU_05850
uniprot id: O05509
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Transcriptional repressor of the gmuBACDREFG operon which is involved in the uptake and degradation of glucomannan.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system.

224308:BSU 20500
gene id: 224308:BSU_20500
uniprot id: O31917
names: Similarity
messages: Belongs to the ATP-dependent DNA ligase family.

224308:BSU 25000
gene id: 224308:BSU_25000
uniprot id: A0A6M4JME5
uniprot id: P54488
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: Expressed in vegetative cells. Also present in dormant spores in the forespore inner membrane.
messages: Forespore inner membrane
messages: Involved in the synthesis of peptidoglycan associated with cell wall elongation, especially following spore germination (PubMed:9139922). Has a partially redundant function with PBP 1 (ponA) or PBP 4 (pbpD) during spore outgrowth (PubMed:9851991). Plays a redundant role with PbpH in determining the rod shape of the cell during vegetative growth and spore outgrowth (PubMed:12896990).
messages: No visible phenotype during vegetative growth rate in rich media, no difference in sporulation or initial spore germination. Spores have a significant delay in outgrowth, between 60-90 minutes cells swell rather than elongate, upon eventual elongation they have a larger diameter and are often bent (PubMed:9139922, PubMed:9851991). Single deletions have reduced peptidoglycan (PG) synthesis and turnover rates. Double ponA-pbpA deletions spores have greatly decreased viability, PG synthesis and elongate poorly; increased levels of Mg(2+) increase spore viability. Double pbpA-pbpD deletions spores have greatly decreased spore outgrowth and peptidoglycan synthesis (PubMed:9851991). Single pbpA mutants are less resistant to ceftriaxone and mecillinam. Double pbpA-pbpH mutants cannot be made, suggesting the 2 proteins have redundant, essential roles in vegetative growth (PubMed:12896990).
messages: Transcribed during vegetative phase, drops off during sporulation, rises again 30-40 minutes after germination. A small amount is present in dormant spores.

224308:BSU 36890
gene id: 224308:BSU_36890
uniprot id: A0A6M3ZI06
uniprot id: P39149
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
messages: Allosterically activated by GTP.
messages: Belongs to the UPRTase family.
messages: Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.
messages: Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.

224308:BSU 35730
gene id: 224308:BSU_35730
uniprot id: P13484
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
messages: Catalyzes the addition of glucose to the C-2 hydroxy group of the glycerol units in teichoic acid.
messages: Cytoplasm
messages: Not essential. No glucose groups on the glycerol residues of teichoic acid in cell walls.
messages: Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.

224308:BSU 15990
gene id: 224308:BSU_15990
uniprot id: A0A6M3ZE91
uniprot id: P21474
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bS16 family.
messages: Part of the 30S ribosomal subunit.

224308:BSU 38730
gene id: 224308:BSU_38730
uniprot id: A0A6M3ZHU6
uniprot id: P94367
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Cysteine exporter (TC 3.A.1.129.1) family.
messages: Cell membrane
messages: Forms a heterodimer with CydC.
messages: In CydD the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.
messages: Membrane
messages: Part of the ABC transporter complex CydDC that exports the reduced low-molecular-weight thiols cysteine and glutathione from the cell. Export of these thiol-containing redox-active molecules may be crucial for redox homeostasis, permitting correct assembly of various respiratory complexes and formation of correct disulfide bonds in secreted proteins. CydD contains transmembrane domains (TMD), which form a pore in the membrane, and an ATP-binding domain (NBD), which is responsible for energy generation.

224308:BSU 33390
gene id: 224308:BSU_33390
uniprot id: A0A6M3ZGL9
uniprot id: O32209
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane.
messages: could be part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane.

224308:BSU 21000
gene id: 224308:BSU_21000
uniprot id: O31941
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 15940
gene id: 224308:BSU_15940
uniprot id: A0A6M3ZAX9
uniprot id: P51834
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SMC family.
messages: Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible SMC hinge near the middle of the molecule. These domains are separated by coiled-coil structures.
messages: Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.
messages: Cytoplasm
messages: Homodimer.
messages: Mutants produce anucleate cells and show defects in nucleoid structure and in chromosome partitioning.
messages: Required for chromosome condensation and partitioning.

224308:BSU 28090
gene id: 224308:BSU_28090
uniprot id: A0A6M3ZIS2
uniprot id: Q05873
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner (By similarity). Catalyzes the attachment of valine to tRNA(Val).
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.
messages: By valine starvation. Is transcribed by itself and in an operon with folC. A GUC triplet (Val, the specifier codon) 190 nucleotides upstream of the initiator codon confers induction upon valaine starvation; replacing it with ACC (Thr) confers induction upon threonine starvation, replacing it with UAA (stop), renders the gene uninducible. Negatively regulates its own transcription; this depends on the presence of the GUC specifier codon.
messages: Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.
messages: Cells lacking this gene grow normally, suggesting there is a second functional gene with valine-tRNA synthetase activity in B.subtilis.
messages: Cytoplasm
messages: Monomer.
messages: The C-terminal coiled-coil domain is crucial for aminoacylation activity.
messages: ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.

224308:BSU 16400
gene id: 224308:BSU_16400
uniprot id: A0A6M3ZED1
uniprot id: Q01960
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GTP-binding SRP family.
messages: Cell membrane
messages: Membrane
messages: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.

224308:BSU 18170
gene id: 224308:BSU_18170
uniprot id: A0A6M3ZB90
uniprot id: O31821
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GtrA family.
messages: Cell membrane
messages: Membrane

224308:BSU 16650
gene id: 224308:BSU_16650
uniprot id: A0A6M4JJ61
uniprot id: P32731
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RbfA family.
messages: Cytoplasm
messages: Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.
messages: One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.

224308:BSU 31060
gene id: 224308:BSU_31060
uniprot id: A0A6M3ZFF7
uniprot id: P71016
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Activity is stimulated by low concentrations of salts and by moderate concentrations of glycine betaine. Highly tolerant to high ionic conditions. In vitro, activity is highly stimulated in the presence of proline.
messages: Belongs to the aldehyde dehydrogenase family.
messages: By choline and by high osmolarity in the presence of choline (PubMed:8752328, PubMed:22408163). Repressed by GbsR (PubMed:22408163).
messages: Deletion of the gbsAB genes abolishes the choline-glycine betaine synthesis pathway and the ability of B.subtilis to deal effectively with high-osmolarity stress in choline- or glycine betaine aldehyde-containing medium (PubMed:8752328). No effect on vanillin degradation (PubMed:26658822).
messages: Homodimer.
messages: Involved in the biosynthesis of the osmoprotectant glycine betaine from choline (PubMed:8752328, PubMed:9297465). Catalyzes the oxidation of betaine aldehyde to betaine (PubMed:9297465). Shows specificity for betaine aldehyde as substrate. Can use both NAD(+) and NADP(+), but NAD(+) is strongly preferred (PubMed:9297465).

224308:BSU 31500
gene id: 224308:BSU_31500
uniprot id: P40761
names: Similarity
messages: Belongs to the DCC thiol-disulfide oxidoreductase family.

224308:BSU 04690
gene id: 224308:BSU_04690
uniprot id: P42411
names: Function
messages: Provides the crucial link between the upstream module (communication of environmental stress) and the downstream module (integration of the environmental signals with signals of energy stress) that compose the signal transduction pathway controlling the sigma-B factor. Phosphorylates and inactivates its specific antagonist protein RsbS thanks to its serine kinase activity. Upon phosphorylation of RsbS, RsbT is released to stimulate RsbU, a PP2C phosphatase, thereby initiating the signaling cascade that ultimately activates sigma-B. The activity of the RsbU phosphatase may be stimulated by a long-lived interaction with RsbT and the serine kinase function of RsbT is not required to directly modify RsbU. Also phosphorylates RsbR thanks to its threonine kinase activity, preventing it to phosphorylate RsbT.

224308:BSU 11370
gene id: 224308:BSU_11370
uniprot id: A0A6M4JHD5
uniprot id: P42065
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transporting tripeptides. Probably responsible for energy coupling to the transport system.

224308:BSU 37090
gene id: 224308:BSU_37090
uniprot id: A0A6M4JQ71
uniprot id: Q03224
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FBPase class 2 family.
messages: Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. Can functionally substitute for the FBPase class 3 (Fbp) of B.subtilis.
messages: Cytoplasm
messages: Disruption of ywjI alone (or fbp alone) does not affect growth on various carbon sources. But the ywjI fbp double mutant is unable to grow with carbon sources demanding FBPase activity, i.e. glycerol, malate, and a mixture of succinate and glutamate.
messages: Expressed at the same level under glycolytic or gluconeogenic conditions.
messages: Inhibited by phosphoenolpyruvate (PEP).

224308:BSU 18800
gene id: 224308:BSU_18800
uniprot id: A0A6M4JIZ5
uniprot id: P39824
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-A beta-lactamase family.
messages: Secreted
messages: This protein is a beta-lactamase with a substrate specificity for penicillins.

224308:BSU 16700
gene id: 224308:BSU_16700
uniprot id: P50850
names: Similarity
messages: Belongs to the polysaccharide deacetylase family.

224308:BSU 01150
gene id: 224308:BSU_01150
uniprot id: A0A6M3Z773
uniprot id: P21471
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS10 family.
messages: Involved in the binding of tRNA to the ribosomes.
messages: Part of the 30S ribosomal subunit.

224308:BSU 24960
gene id: 224308:BSU_24960
uniprot id: P46341
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.
messages: Cell membrane
messages: Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).

224308:BSU 12310
gene id: 224308:BSU_12310
uniprot id: A0A6M3Z9M9
uniprot id: O34961
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:galactoside symporter (TC 2.A.2) family.
messages: Cell membrane
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.
messages: Membrane

224308:BSU 31110
gene id: 224308:BSU_31110
uniprot id: O32082
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 18390
gene id: 224308:BSU_18390
uniprot id: O35009
names: Similarity
messages: Belongs to the 'phage' integrase family.

224308:BSU 12830
gene id: 224308:BSU_12830
uniprot id: O34853
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SpoIISA toxin family.
messages: Cell membrane
messages: Expressed at low levels during exponential growth, it increases dramatically at the onset of sporulation (at protein level). A member of the spoIISA-spoIISB operon.
messages: No change in sporulation efficiency.
messages: Probably forms an oligomer; X-ray data suggests the inactive complex forms a heterotetramer of SpoIISA(2)-SpoIISB(2), which inactivates the toxic activity of SpoIISA.
messages: Toxic component of a type II toxin-antitoxin (TA) system. Its toxic activity is neutralized by cognate antitoxin SpoIISB. Expression in the absence of SpoIISB permits sporulation to stage II, when plasmolysis zones and holes in the peptidoglycan layer are observed, resulting in cell death. Lethal when synthesized during vegetative growth in the absence of SpoIISB. In E.coli both the membrane bound and soluble domain are required in cis for toxin activity.

224308:BSU 07460
gene id: 224308:BSU_07460
uniprot id: A0A6M3ZBN7
uniprot id: O34440
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane

224308:BSU 24200
gene id: 224308:BSU_24200
uniprot id: P40770
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32740
gene id: 224308:BSU_32740
uniprot id: A0A6M3ZG63
uniprot id: O32168
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex MetNPQ involved in methionine import. Responsible for the translocation of the substrate across the membrane (Probable). It has also been shown to be involved in methionine sulfoxide transport.
messages: Repressed by methionine via the S-box system.
messages: The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetP) and a solute-binding protein (MetQ).

224308:BSU 33100
gene id: 224308:BSU_33100
uniprot id: O32199
names: Disruption Phenotype
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Induced, via the two-component regulatory system LiaS/LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.
messages: Up-regulates expression of liaI which in turn induces the liaRS two-component regulatory system.

224308:BSU 36670
gene id: 224308:BSU_36670
uniprot id: A0A6M4JQ00
uniprot id: P70964
names: Induction
names: Similarity
messages: Belongs to the UPF0337 (CsbD) family.
messages: By phosphate starvation, via the alternative sigma factor sigma-B.

224308:BSU 14360
gene id: 224308:BSU_14360
uniprot id: A0A6M3ZA95
uniprot id: O31711
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Expressed in exponential-phase cells.
messages: Part of a complex composed of YknX, YknY and YknZ. The complex interacts with YknW.
messages: Part of an unusual four-component transporter, which is required for protection against the killing factor SdpC (sporulation-delaying protein).

224308:BSU 29990
gene id: 224308:BSU_29990
uniprot id: A0A6M3ZFL9
uniprot id: O34978
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.
messages: Cell membrane
messages: Expression is regulated by the purR regulon. Down-regulated by adenine and up-regulated by guanosine.
messages: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM.
messages: Membrane

224308:BSU 01230
gene id: 224308:BSU_01230
uniprot id: A0A6M3Z6Y3
uniprot id: P14577
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL16 family.
messages: Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.
messages: Part of the 50S ribosomal subunit.

224308:BSU 12360
gene id: 224308:BSU_12360
uniprot id: A0A6M4JHK6
uniprot id: O34456
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Phthalate permease family.
messages: Cell membrane
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.
messages: Membrane
messages: Transport of aldohexuronates such as D-glucuronate and D-galacturonate.

224308:BSU 23820
gene id: 224308:BSU_23820
uniprot id: A0A6M3ZDT0
uniprot id: P54550
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.
messages: By toxic xenobiotic compounds (2,4,6-trinitrotoluene and nitroglycerin), and in response to oxidative stress (hydrogen peroxide and paraquat).
messages: Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.
messages: Forms a charge transfer complex with a variety of phenolic compounds.
messages: Homotetramer.
messages: Homotetramer. Composed of a dimer of active dimers.
messages: Inhibited by p-hydroxybenzaldehyde (pHBA) and p-nitrophenol (pNP).

224308:BSU 12120
gene id: 224308:BSU_12120
uniprot id: O34438
names: Induction
messages: Transcriptionally regulated by SigD.

224308:BSU 31890
gene id: 224308:BSU_31890
uniprot id: A0A6M4JL52
uniprot id: P71070
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EssB family.
messages: Cell membrane
messages: Cells lacking this gene are blocked in YukE secretion (PubMed:23861817, PubMed:24798022). They display an increased bacteriophage SPP1 resistance phenotype (PubMed:15576783). Loss of delivery of LXG toxins to target cells (PubMed:34280190).
messages: Membrane
messages: Required for YukE secretion. Probable component or regulator of the ESX/ESAT-6-like secretion system (BsEss) (PubMed:23861817, PubMed:24798022). Required to deliver LXG toxins to target cells (PubMed:34280190).

224308:BSU 00530
gene id: 224308:BSU_00530
uniprot id: A0A6M3Z720
uniprot id: P37470
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PTH family.
messages: Cytoplasm
messages: Monomer.
messages: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.

224308:BSU 38290
gene id: 224308:BSU_38290
uniprot id: A0A6M3ZLK0
uniprot id: P39594
names: Cofactor
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the thiamine-phosphate synthase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).
messages: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.
messages: Is weakly repressed by THZ and not at all by thiamine.
messages: Monomer.
messages: Thiamine phosphate synthase appears to proceed with a dissociative mechanism (SN1 like) in which the pyrimidine pyrophosphate dissociates to give a reactive pyrimidine intermediate which is then trapped by the thiazole moiety.

224308:BSU 03540
gene id: 224308:BSU_03540
uniprot id: Q08793
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 40040
gene id: 224308:BSU_40040
uniprot id: A0A6M4JR29
uniprot id: P42100
names: Similarity
messages: Belongs to the glycerate kinase type-1 family.

224308:BSU 03000
gene id: 224308:BSU_03000
uniprot id: P46922
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine.
messages: Membrane raft
messages: The complex is composed of two ATP-binding proteins (OpuAA), two transmembrane proteins (OpuAB) and a solute-binding protein (OpuAC) (PubMed:7622480). Interacts with FloT (PubMed:23651456).

224308:BSU 00730
gene id: 224308:BSU_00730
uniprot id: A0A6M4JEM7
uniprot id: P37887
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the cysteine synthase/cystathionine beta-synthase family.
messages: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression.
messages: Grows very slowly with sulfate, butanesulfonate or cystine as sole sulfur source.
messages: Highly expressed in the presence of methionine, but poorly expressed in the presence of cystine. Also induced by superoxide. Repressed both by sulfate and cysteine.
messages: Homodimer. Forms CymR(2):CysK(2) or CymR(4):CysK(4) complexes in the absence of O-acetylserine.

224308:BSU 06380
gene id: 224308:BSU_06380
uniprot id: O34341
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31050
gene id: 224308:BSU_31050
uniprot id: P71017
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the iron-containing alcohol dehydrogenase family.
messages: By choline and by high osmolarity in the presence of choline (PubMed:8752328, PubMed:22408163). Repressed by GbsR (PubMed:22408163).
messages: Deletion of the gbsAB genes abolishes the choline-glycine betaine synthesis pathway and the ability of B.subtilis to deal effectively with high-osmolarity stress in choline- or glycine betaine aldehyde-containing medium.
messages: Involved in the biosynthesis of the osmoprotectant glycine betaine from choline.

224308:BSU 23580
gene id: 224308:BSU_23580
uniprot id: A0A6M3ZDP1
uniprot id: P26900
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
messages: Belongs to the asparaginase 1 family.
messages: Cytoplasm
messages: Expression is induced by asparagine.
messages: Homotetramer.
messages: Produced in 2 x SG medium but not in 2 x YT medium.
messages: Produced in 2 x YT medium but not in 2 x SG medium.

224308:BSU 27450
gene id: 224308:BSU_27450
uniprot id: A0A6M4JK77
uniprot id: O34671
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex GlnHMPQ involved in glutamine transport. Probably responsible for the translocation of the substrate across the membrane (By similarity).
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: The complex is composed of two ATP-binding proteins (GlnQ), two transmembrane proteins (GlnM and GlnP) and a solute-binding protein (GlnH).

224308:BSU 13190
gene id: 224308:BSU_13190
uniprot id: A0A6M3Z9W4
uniprot id: P11018
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase S8 family.
messages: Cytoplasm
messages: Homodimer.
messages: Major intracellular protease produced by Bacillus subtilis.

224308:BSU 06811
gene id: 224308:BSU_06811
uniprot id: C0H3X4
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the yeeF-yezG operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YeeF (PubMed:34280190). Probably neutralizes the ability to inhibit growth of cognate toxin YeeF. Probably does not have immunity protein activity on other LXG toxins (Probable).
messages: Interacts with cognate toxin YeeF but not with non-cognate toxin YobL. The interaction probably inhibits the toxic activity of YeeF (PubMed:22200572). May bind with a stoichiometry of 2:2 to YeeF (By similarity).

224308:BSU 24230
gene id: 224308:BSU_24230
uniprot id: P17896
names: Activity Regulation
names: Developmental Stage
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Forespore intermembrane space
messages: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by cis cleavage to form SpoIVB-43 kDa and SpoIVB-42 kDa. The protease then cleaves the C-terminus of the SpoIVFA metalloprotease activating the latter.
messages: The PDZ domain mediates interaction with another SpoIVB protein during transactivation. Also mediates interaction with BofA during cleavage of SpoIVFA.
messages: The zymogen is inhibited from undergoing autoactivation by BofC. The protease is inactivated by proteolytic cleavage.
messages: Transcribed in low amounts by sigma-F. Transcribed during stage III of sporogenesis by sigma-G.

224308:BSU 14900
gene id: 224308:BSU_14900
uniprot id: A0A6M3ZAZ5
uniprot id: P24010
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the heme-copper respiratory oxidase family.
messages: Binds 1 copper B ion per subunit.
messages: Binds 2 heme groups per subunit.
messages: Cell membrane
messages: Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
messages: Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.
messages: Membrane

224308:BSU 24620
gene id: 224308:BSU_24620
uniprot id: P54507
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M73 family.
messages: Forespore intermembrane space
messages: Forms fibers (PubMed:20080671, PubMed:29531041). Fibers have variable length and are 10-15 nm width (PubMed:20080671). Interacts with obg (AC P20964) in pull-down experiments (PubMed:12429099).
messages: In laboratory strains, is a transition-phase protein that is expressed early in spore formation, as cells enter stationary phase (PubMed:10049401, PubMed:10368135, PubMed:10464223). Expression can occur as cells enter stationary phase even under sporulation-repressing conditions (PubMed:10464223).
messages: Mutation impairs colony surface architecture (PubMed:16430695). Mutant forms pellicles with less extracellular material (PubMed:16430696). Deletion of the gene results in the production of asymmetric spores that accumulate misassembled material in one pole and have a greatly expanded undercoat and an altered outer coat structure (PubMed:10368135).
messages: Part of the tapA-sipW-tasA operon (PubMed:10464223). Expression is directly repressed by the DNA-binding protein master regulator of biofilm formation SinR and activated by the extracellular matrix regulatory protein RemA (PubMed:16430695, PubMed:23646920). Also positively regulated by the sporulation transcription factors sigma H and Spo0A and repressed by the transition phase regulatory protein AbrB, probably indirectly (PubMed:10049401, PubMed:10368135, PubMed:10464223).
messages: Secreted
messages: TasA is the major protein component of the biofilm extracellular matrix (PubMed:16430696, PubMed:20080671). It forms amyloid fibers that bind cells together in the biofilm (PubMed:20080671). Exhibits an antibacterial activity against a variety of Gram-positive and Gram-negative bacteria (PubMed:10049401). In laboratory strains, is also involved in proper spore coat assembly (PubMed:10368135).

224308:BSU 00090
gene id: 224308:BSU_00090
uniprot id: A0A6M3Z9J0
uniprot id: P21879
names: Activity Regulation
names: Caution
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the IMPDH/GMPR family.
messages: By superoxide.
messages: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.

224308:BSU 04010
gene id: 224308:BSU_04010
uniprot id: A0A6M3Z7Q4
uniprot id: P42959
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.
messages: Belongs to the peptidase S26 family.
messages: Cell membrane
messages: Expressed constitutively.
messages: Membrane

224308:BSU 13010
gene id: 224308:BSU_13010
uniprot id: A0A6M3ZDD7
uniprot id: O34499
names: Function
names: Similarity
messages: Belongs to the cycloisomerase 2 family.
messages: Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.

224308:BSU 34280
gene id: 224308:BSU_34280
uniprot id: P71059
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Repressed by SinR.

224308:BSU 26010
gene id: 224308:BSU_26010
uniprot id: P45950
names: Similarity
messages: To B.subtilis XkdQ.

224308:BSU 22560
gene id: 224308:BSU_22560
uniprot id: P46911
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Rieske iron-sulfur protein family.
messages: Binds 1 [2Fe-2S] cluster per subunit.
messages: Component of the menaquinol:cytochrome c reductase complex. The Rieske protein is a high potential 2Fe-2S protein.
messages: The main subunits of the menaquinol:cytochrome c complex are a Rieske-type iron-sulfur protein (QcrA), a cytochrome b (QcrB) and a cytochrome c (QcrC).

224308:BSU 37340
gene id: 224308:BSU_37340
uniprot id: O07624
names: Domain
names: Subunit
messages: Forms a beta-barrel structure that may accommodate hydrophobic ligands.
messages: Homodimer.

224308:BSU 24900
gene id: 224308:BSU_24900
uniprot id: A0A6M4JMD8
uniprot id: P56849
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL33 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Interacts with VmlR (PubMed:30126986).

224308:BSU 04550
gene id: 224308:BSU_04550
uniprot id: A0A6M4JDI1
uniprot id: P96611
names: Function
names: Induction
names: Similarity
messages: Activated by SigB in response to ethanol stress.
messages: Belongs to the thioredoxin family.
messages: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.

224308:BSU 12990
gene id: 224308:BSU_12990
uniprot id: A0A6M3Z9U6
uniprot id: O35010
names: Function
names: Induction
names: Similarity
messages: Belongs to the peptidase C40 family.
messages: Repressed by AbrB, a transcription factor that negatively controls biofilm formation.
messages: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu.

224308:BSU 23520
gene id: 224308:BSU_23520
uniprot id: A0A6M4JIV0
uniprot id: P54574
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Fur family.
messages: Binds 1 Mn(2+) or Fe(2+) ion per subunit.
messages: Binds 1 zinc ion per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: Iron uptake repressor. Acts on the transcription of ferri-siderophore uptake genes.

224308:BSU 03620
gene id: 224308:BSU_03620
uniprot id: A0A6M4JFF1
uniprot id: P94403
names: Function
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.
messages: Could be a positive regulator of bsdBCD expression in response to salicylic acid.

224308:BSU 03450
gene id: 224308:BSU_03450
uniprot id: A0A6M3Z7T3
uniprot id: P42404
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the SIS family. PHI subfamily.
messages: By formaldehyde, under the control of HxlR. Not induced by methanol, formate, or methylamine.
messages: Catalyzes the isomerization between 3-hexulose 6-phosphate and fructose 6-phosphate. Together with HxlA, may act as a formaldehyde detoxification system.
messages: Homotetramer.

224308:BSU 29610
gene id: 224308:BSU_29610
uniprot id: O34894
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EzrA family.
messages: Cell membrane
messages: May be degraded by FtsH protease.
messages: Membrane raft
messages: Negative regulator of FtsZ ring formation; modulates the frequency and position of FtsZ ring formation. Inhibits FtsZ ring formation at polar sites. Interacts either with FtsZ or with one of its binding partners to promote depolymerization.
messages: Suppresses temperature-sensitive mutation in FtsZ, in a wild-type FtsZ background increases the number and alters the position of FtsZ rings (PubMed:10449747). In minimal medium cells elongate and occasionally form minicells. Double dynA-ezrA mutants have longer cells with more double septa than either deletion alone (PubMed:23249255).

224308:BSU 33890
gene id: 224308:BSU_33890
uniprot id: O32248
names: Function
messages: Probable N-acetyltransferase.

224308:BSU 04450
gene id: 224308:BSU_04450
uniprot id: A0A6M3Z7K0
uniprot id: P96601
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system DctS/DctR. Probably activates DctR by phosphorylation (By similarity). Essential for expression of dctP.
messages: Membrane

224308:BSU 11530
gene id: 224308:BSU_11530
uniprot id: O31604
names: Developmental Stage
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Preferentially expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:17630974).
messages: Required for optimal transformation.

224308:BSU 38000
gene id: 224308:BSU_38000
uniprot id: P39612
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31100
gene id: 224308:BSU_31100
uniprot id: A0A6M4JNZ4
uniprot id: O32081
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TrkH potassium transport family. Ktr (TC 2.A.38.4) subfamily.
messages: Cell membrane
messages: Homodimer. Part of the KtrAB complex formed by an octameric catalytic ring of KtrA and a membrane associated dimer of KtrB forming a potassium channel.
messages: Impaired potassium uptake.
messages: Integral membrane subunit of the KtrAB potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.
messages: Membrane

224308:BSU 11590
gene id: 224308:BSU_11590
uniprot id: A0A6M3Z9I0
uniprot id: O31610
names: Similarity
messages: Belongs to the UPF0738 family.

224308:BSU 09870
gene id: 224308:BSU_09870
uniprot id: O07534
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Modulates the activity of the potassium/proton antiporter KhtU (PubMed:14987767). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).
messages: Part of the khtSTU operon. Induced by salt stress at alkaline pH.

224308:BSU 06559
gene id: 224308:BSU_06559
uniprot id: C0H3X3
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36800
gene id: 224308:BSU_36800
uniprot id: A0A6M3ZHD6
uniprot id: P37812
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase epsilon chain family.
messages: Cell membrane
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
messages: Produces ATP from ADP in the presence of a proton gradient across the membrane.

224308:BSU 00120
gene id: 224308:BSU_00120
uniprot id: A0A6M3Z6Y4
uniprot id: P37528
names: Function
names: Similarity
names: Subunit
messages: Belongs to the glutaminase PdxT/SNO family.
messages: Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.
messages: In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.

224308:BSU 34780
gene id: 224308:BSU_34780
uniprot id: O06972
names: Disruption Phenotype
names: Similarity
messages: Belongs to the Nudix hydrolase family.
messages: Cells lacking this gene have an unchanged spontaneous mutation frequency, even in triple mutT/yjhB/yvcI disruptions.

224308:BSU 26710
gene id: 224308:BSU_26710
uniprot id: A0A6M3ZHM5
uniprot id: O07942
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AzlC family.
messages: Cell membrane
messages: Involved in branched-chain amino acid transport.
messages: Membrane

224308:BSU 30670
gene id: 224308:BSU_30670
uniprot id: A0A6M3ZFB4
uniprot id: O34667
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the LuxS family.
messages: Binds 1 Fe cation per subunit.
messages: Homodimer.
messages: Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).

224308:BSU 36490
gene id: 224308:BSU_36490
uniprot id: P94573
names: Similarity
messages: Belongs to the isochorismatase family.

224308:BSU 07000
gene id: 224308:BSU_07000
uniprot id: O31521
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 105 family.
messages: Catalyzes the hydrolysis of unsaturated rhamnogalacturonan disaccharide to yield unsaturated D-galacturonic acid and L-rhamnose. It cannot act on unsaturated glucuronyl hydrolase (UGL) substrates containing unsaturated D-glucuronic acid at the non-reducing terminus, although the active pockets of YesR and UGL are very similar.
messages: Cytoplasm
messages: Monomer.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 12180
gene id: 224308:BSU_12180
uniprot id: O34725
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36690
gene id: 224308:BSU_36690
uniprot id: P70962
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activity is partially inhibited by the competence and sporulation stimulating factor (CSF), encoded by phrC (PubMed:9238025). Phosphatase activity is abolished in the presence of EDTA (PubMed:11923303). Insensitive to vanadate (PubMed:9843420).
messages: Belongs to the Rap family.
messages: Can also use Mg(2+), Ca(2+) and Zn(2+), with lower efficiency.
messages: Cytoplasm
messages: Homodimer (PubMed:11923303). Interacts with Spo0F (PubMed:9843420).
messages: Involved in the regulation of sporulation (PubMed:8001132, PubMed:9843420). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:8001132, PubMed:11923303, PubMed:9843420).

224308:BSU 30710
gene id: 224308:BSU_30710
uniprot id: A0A6M4JKR8
uniprot id: C0SP90
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome ubiquinol oxidase subunit 1 family.
messages: Binds 1 protoheme IX group (heme b).
messages: Cell membrane
messages: May have a role in sporulation. Can compensate for the loss of cytochrome aa3.
messages: Membrane
messages: Not essential.

224308:BSU 15350
gene id: 224308:BSU_15350
uniprot id: A0A6M3ZAJ1
uniprot id: O31724
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the peptidase M20A family.
messages: Binds 2 Zn(2+) or Co(2+) ions per subunit.
messages: Catalyzes the deformylation of the formylaminopyrimidine N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine (FAMP) to give the corresponding aminopyrimidine.

224308:BSU 29160
gene id: 224308:BSU_29160
uniprot id: A0A6M4JNX3
uniprot id: O34991
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.
messages: Cell membrane
messages: Membrane

224308:BSU 05529
gene id: 224308:BSU_05529
uniprot id: C0H3W2
names: Similarity
messages: Belongs to the GerPA/GerPF family.

224308:BSU 16210
gene id: 224308:BSU_16210
uniprot id: A0A6M4JMY9
uniprot id: P23447
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum basal body
messages: Belongs to the FliF family.
messages: Cell membrane
messages: Membrane
messages: The M ring may be actively involved in energy transduction.
messages: The basal body constitutes a major portion of the flagellar organelle and consists of two rings, one in the peptidoglycan layer and one in the plasma membrane.

224308:BSU 40550
gene id: 224308:BSU_40550
uniprot id: A0A6M4JR90
uniprot id: P37487
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PPase class C family.
messages: Binds 2 manganese ions per subunit.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 16190
gene id: 224308:BSU_16190
uniprot id: A0A6M3ZIA9
uniprot id: P24501
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum basal body
messages: Belongs to the flagella basal body rod proteins family.
messages: The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each (By similarity).
messages: The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.

224308:BSU 38440
gene id: 224308:BSU_38440
uniprot id: P25149
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 12860
gene id: 224308:BSU_12860
uniprot id: A0A6M3Z9U7
uniprot id: O34739
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
messages: Cell membrane
messages: Exhibits an obligate exchange activity for serine, threonine and aromatic amino acids.
messages: Membrane
messages: Monomer.

224308:BSU 17110
gene id: 224308:BSU_17110
uniprot id: O34877
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 03360
gene id: 224308:BSU_03360
uniprot id: A0A6M3Z7S3
uniprot id: P94400
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.
messages: No effect in wild-type, but required for optimal growth in strains lacking the ZnuABC zinc uptake system.
messages: Repressed by zinc via the metallo-regulatory protein Zur.
messages: Zinc chaperone that directly transfers zinc cofactor to target proteins, thereby activating them (By similarity). Zinc is transferred from the CXCC motif in the GTPase domain to the zinc binding site in target proteins in a process requiring GTP hydrolysis (By similarity). Contributes to optimal growth under starvation for zinc (PubMed:12426338).

224308:BSU 23070
gene id: 224308:BSU_23070
uniprot id: A0A6M3ZIQ1
uniprot id: P35136
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
messages: Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.
messages: In bacteria displays feedback inhibition by L-serine.

224308:BSU 37950
gene id: 224308:BSU_37950
uniprot id: P39617
names: Induction
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 06440
gene id: 224308:BSU_06440
uniprot id: A0A6M3ZBB6
uniprot id: P12047
names: Function
names: Similarity
names: Subunit
messages: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.
messages: Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate (PubMed:15182182). Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability.
messages: Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.

224308:BSU 30120
gene id: 224308:BSU_30120
uniprot id: O34559
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 105 family.
messages: Catalyzes the hydrolysis of unsaturated rhamnogalacturonan disaccharide to yield unsaturated D-galacturonic acid and L-rhamnose. It cannot act on unsaturated glucuronyl hydrolase (UGL) substrates containing unsaturated D-glucuronic acid at the non-reducing terminus, although the active pockets of YesR and UGL are very similar.
messages: Cytoplasm
messages: Monomer.

224308:BSU 01330
gene id: 224308:BSU_01330
uniprot id: A0A6M4JRE7
uniprot id: P21467
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Altered S5 proteins have been identified in a number of mutants. Some mutations in S5 have been shown to increase translational error frequencies.
messages: Belongs to the universal ribosomal protein uS5 family.
messages: Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts proteins S4 and S8 (By similarity).
messages: Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.
messages: The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.
messages: With S4 and S12 plays an important role in translational accuracy.
messages: With S4 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).

224308:BSU 21300
gene id: 224308:BSU_21300
uniprot id: O31971
names: Caution
names: Similarity
messages: Although strongly related to the 'phage' integrase family this protein lacks the conserved Tyr at position 318 suggesting it has no recombinase activity.
messages: Belongs to the 'phage' integrase family.

224308:BSU 18190
gene id: 224308:BSU_18190
uniprot id: O31823
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DedA family.
messages: Cell membrane
messages: Deletion of the gene increases sensitivity to the amphomycin derivative MX2401, which binds UndP in the outer leaflet of the cytoplasmic membrane and prevents its recycling (PubMed:36450357). Deletion of the gene sensitizes B.subtilis to reduced levels of UndP synthesis (PubMed:36450357). The double mutant uptA-ykoX exhibits increased sensitivity to the amphomycin derivative MX2401, but has no discernible growth or morphological defects (PubMed:36450357).
messages: Expression is regulated by the ECF sigma factor SigM.
messages: Flippase that catalyzes the transport of undecaprenyl phosphate (UndP) across the cytoplasmic membrane, from the external side to the cytoplasmic side (PubMed:36450357). Is involved in UndP recycling during peptidoglycan synthesis (PubMed:36450357).

224308:BSU 00390
gene id: 224308:BSU_00390
uniprot id: P37545
names: Cofactor
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.
messages: Binds 2 divalent metal cations per subunit.

224308:BSU 04560
gene id: 224308:BSU_04560
uniprot id: P96612
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the D-alanine--D-alanine ligase family.
messages: Binds 2 magnesium or manganese ions per subunit.
messages: Cell wall formation.
messages: Cytoplasm

224308:BSU 11630
gene id: 224308:BSU_11630
uniprot id: A0A6M3Z9U8
uniprot id: O31614
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP.
messages: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP. Does not hydrolyze Ap2a or Ap6A. Also has an ATPase activity. Was shown to dephosphorylate phosphotyrosine but not phosphoserine or phosphothreonine from phosphorylated peptides. Involved in spore germination by controlling expression of genes coding for GerA and GerK receptors.
messages: Belongs to the PrpE family.
messages: Forespore
messages: Inhibited by EDTA.
messages: Nickel. 100-fold less efficiency with manganese.

224308:BSU 20270
gene id: 224308:BSU_20270
uniprot id: A0A6M3ZCU7
uniprot id: P68522
names: Function
names: Similarity
messages: Belongs to the 5'(3')-deoxyribonucleotidase family.
messages: Dephosphorylates nucleoside monophosphates such as the 5' and 2'(3')-phosphates of deoxyribonucleotides in vitro. Also catalyzes the dephosphorylation of coenzyme A (CoA), pyridoxal-5'-phosphate (PLP), riboflavine-5-phosphate (FMN) and nicotinamide adenine dinucleotide phosphate (NADP) in vitro.

224308:BSU 22710
gene id: 224308:BSU_22710
uniprot id: A3F3D4
uniprot id: P31104
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the chorismate synthase family.
messages: Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.
messages: Homotetramer.
messages: Reduced FMN (FMNH(2)).

224308:BSU 18380
gene id: 224308:BSU_18380
uniprot id: O34841
names: Induction
messages: Negatively regulated by the two-component system YycFG.

224308:BSU 01540
gene id: 224308:BSU_01540
uniprot id: P50863
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Autoregulated.
messages: Belongs to the Mrp/NBP35 ATP-binding proteins family.
messages: Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.
messages: Homodimer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Negatively regulates the expression of hpr/scoC. The effect on hpr/scoC may be indirect.

224308:BSU 24950
gene id: 224308:BSU_24950
uniprot id: P46342
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.
messages: Cell membrane
messages: Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).

224308:BSU 22950
gene id: 224308:BSU_22950
uniprot id: P50736
names: Function
names: PTM
names: Subunit
messages: C-terminal Cys can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation protects Cys residues against overoxidation by acting as a redox switch in response to oxidative stress.
messages: Interacts with BrxC.
messages: S-bacillithiolation is the formation of mixed disulfide bonds between protein thiols and the general thiol reductant bacillithiol (BSH) under oxidative stress. BSH is an equivalent of glutathione (GSH) in Firmicutes. This protein is a NADPH-dependent bacilliredoxin reductase, which debacillithiolates (removes BSH) the S-bacillithiolated BrxB (BrxB-SSB), and to a lesser extent BrxC (BrxC-SSB). Involved in a redox cascade increasing the efficacy of BrxB function by reducing BrxB-SSB and thus reactivating it. Has NADPH-dependent oxidase activity under aerobic conditions producing hydrogen peroxide (H(2)O(2)).

224308:BSU 35270
gene id: 224308:BSU_35270
uniprot id: A0A6M3ZGF6
uniprot id: O34594
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
messages: Binds 1 heme c group covalently per subunit.
messages: Cell membrane
messages: Electron carrier protein.
messages: Induced during exponential growth. Repressed by glucose.
messages: Present in very low amounts in the cell.

224308:BSU 31170
gene id: 224308:BSU_31170
uniprot id: O05265
names: Function
names: Induction
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.
messages: Catalyzes the NADPH-dependent reduction of scyllo-inosose (SIS) to scyllo-inositol (SI) in vitro, but is unable to dehydrogenate scyllo-inositol and myo-inositol. Is less efficient than the functional paralog IolW. Under physiological conditions, may primarily function as an NADPH-dependent oxidoreductase that reduces carbonyl group(s) in its substrates. Cannot use NADH instead of NADPH.
messages: Is not induced in the presence of scyllo-inositol or myo-inositol.

224308:BSU 37710
gene id: 224308:BSU_37710
uniprot id: P39641
names: Cofactor
names: Function
names: Induction
names: Subunit
messages: Binds 2 Mg(2+) ions per monomer.
messages: Monomer or homodimer.
messages: Part of the bacABCDEFG operon responsible for the biosynthesis of bacilysin, an irreversible inactivator of the glutaminase domain of glucosamine synthetase. Catalyzes the formation of alpha-dipeptides from various L-amino acids in the presence of ATP. In vivo catalyzes the ligation of L-alanine and L-anticapsin (epoxycyclohexanonyl-Ala) to produce the final bacilysin antibiotic (L-Ala-L-4S-cyclohexenonyl-Ala dipeptide). The substrate specificity is restricted to small amino acids such as L-Ala, for the N-terminal end of the dipeptide, whereas a wide range of hydrophobic amino acids such as L-Phe, L-Tyr and L-Met are recognized for the C-terminal end.
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDE operon and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 02370
gene id: 224308:BSU_02370
uniprot id: O31459
names: Activity Regulation
names: Function
messages: Binding to DNA is allosterically inhibited by the effector glucosamine-6-phosphate (GlcN6P). N-acetylglucosamine-6-phosphate (GlcNAc6P) and glucose-6-phosphate (Glc6P) have no effect.
messages: Transcriptional repressor of genes involved in glucosamine transport and utilization. Represses the expression of the gamAP operon by binding to the gamA-gamR intergenic region.

224308:BSU 03510
gene id: 224308:BSU_03510
uniprot id: A0A6M4JFE2
uniprot id: Q08787
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 1 phosphopantetheine covalently.
messages: Probably activates a leucine.
messages: The phosphoserine observed at Ser-1003 in PubMed:17218307 may result from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.

224308:BSU 05450
gene id: 224308:BSU_05450
uniprot id: P96689
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Up-regulated by mta.

224308:BSU 36130
gene id: 224308:BSU_36130
uniprot id: A0A6M3ZGP2
uniprot id: O05215
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.
messages: Cell membrane
messages: Membrane

224308:BSU 30480
gene id: 224308:BSU_30480
uniprot id: O35008
names: Caution
names: Cofactor
names: Similarity
messages: Belongs to the radical SAM superfamily.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: The spacing between the conserved Cys residues used for the 4Fe-4S cluster is unusual (C-X11-CXXC instead of C-X3-CXXC); it is therefore unclear whether this protein will bind the cofactor.

224308:BSU 16380
gene id: 224308:BSU_16380
uniprot id: A0A6M4JK24
uniprot id: P35538
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the type III secretion exporter family.
messages: Cell membrane
messages: May be involved in the export of flagellum proteins.
messages: Membrane
messages: Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.

224308:BSU 30000
gene id: 224308:BSU_30000
uniprot id: O35029
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05750
gene id: 224308:BSU_05750
uniprot id: Q797E6
names: Cofactor
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Binds 1.0 Fe(2+) (PubMed:20087498) to 1.5 to 3.2 Fe(2+) per monomer (PubMed:21744456).
messages: Cytoplasm
messages: Homodimer, upon Fe(2+) binding (PubMed:20087498). Interacts with the SufS/SufU complex (PubMed:27382962). Interacts with CpfC (PubMed:25826316).
messages: Plays an essential role in iron intracellular trafficking to iron cofactor biogenesis systems including iron-sulfur cluster (Fe-S) or heme assembly (PubMed:21744456, PubMed:25826316). Promotes the biosynthesis of iron-sulfur clusters by delivering Fe to the complex composed of the cysteine desulfurase SufS and the zinc-dependent sulfurtransferase SufU (PubMed:27382962). Also plays a critical role in coproporphyrin-dependent heme b biogenesis and thus provides an essential function for the bacterial global metabolism.
messages: Reduced growth in rich medium, unable to grow in minimal medium. Reduced activity of Fe-S-dependent aconitase (citB). Reactive oxygen species are drastically reduced. No bacillibactin (BB), an endogenous siderophore, produced. 20% higher intracellular iron concentrations.

224308:BSU 04570
gene id: 224308:BSU_04570
uniprot id: A0A6M4JIN4
uniprot id: P96613
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MurCDEF family. MurF subfamily.
messages: Cytoplasm
messages: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.

224308:BSU 02070
gene id: 224308:BSU_02070
uniprot id: P54379
names: Developmental Stage
names: Induction
messages: Expressed in the forespore during sporulation.
messages: Expression is regulated by the sporulation transcription factor sigma G.

224308:BSU 31850
gene id: 224308:BSU_31850
uniprot id: A0A6M3ZIK9
uniprot id: O32100
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EssA family.
messages: Cell membrane
messages: Cells lacking this gene are blocked in YukE secretion.
messages: Required for YukE secretion. Probable component or regulator of the ESX/ESAT-6-like secretion system (BsEss).

224308:BSU 05910
gene id: 224308:BSU_05910
uniprot id: A0A6M4JGW8
uniprot id: O05515
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TsaE family.
messages: Cells lacking this gene exhibit significantly reduced growth in rich and minimal medium.
messages: Cytoplasm
messages: Expressed at all stages of growth.
messages: Monomer, homodimer or homotetramer; in equilibrium. Low salt concentration favors oligomerization, while high salt concentration favors the monomeric form.
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB; this reaction does not require ATP in vitro. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro, which is modulated by the oligomeric status of the protein.
messages: The four proteins YwlC, TsaD, TsaB and TsaE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine biosynthesis in vitro in B.subtilis.
messages: The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in B.subtilis and many other species (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA.

224308:BSU 19740
gene id: 224308:BSU_19740
uniprot id: A0A6M3ZCN7
uniprot id: O34662
names: Similarity
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 07830
gene id: 224308:BSU_07830
uniprot id: A0A6M4JGE8
uniprot id: O34475
names: Similarity
names: Subunit
messages: Belongs to the nitroreductase family.
messages: Monomer.

224308:BSU 19260
gene id: 224308:BSU_19260
uniprot id: A0A6M4JH65
uniprot id: O34321
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the small heat shock protein (HSP20) family.
messages: Cytoplasm
messages: Deletion of the gene increases sensitivity toward salt stress.
messages: Forms homodimers, homotetramers and higher oligomers.
messages: Induced upon salt stress conditions.
messages: Part of the cellular protein quality control system with a specific role in salt stress response. May facilitate protein homeostasis, together with chemical chaperones that accumulate during the salt stress response. Increased levels of YocM protects against both heat and salt stress. In vitro, displays an unusual aggregase chaperone activity.

224308:BSU 38580
gene id: 224308:BSU_38580
uniprot id: A0A6M4JQK6
uniprot id: P46317
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.
messages: Membrane
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lichenan transport.

224308:BSU 39410
gene id: 224308:BSU_39410
uniprot id: A0A6M4JQT5
uniprot id: P39141
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.
messages: Cell membrane
messages: Induced by deoxyadenosine and thymidine. Repressed by DeoR and glucose.
messages: Membrane
messages: Transport of the pyrimidine nucleoside uridine.

224308:BSU 03940
gene id: 224308:BSU_03940
uniprot id: P94431
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Significantly induced under copper-limiting conditions.

224308:BSU 08650
gene id: 224308:BSU_08650
uniprot id: P71079
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). It confers resistance to triclosan.
messages: Cells lacking this gene exhibit a 250-fold decrease in the triclosan MIC.
messages: Expressed during exponential growth.
messages: Homotetramer.
messages: Inhibited by triclosan.

224308:BSU 38540
gene id: 224308:BSU_38540
uniprot id: A0A6M3ZHQ3
uniprot id: P39577
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Cells lacking this gene have no defect in lipoteichoic acid or wall teichoic acid synthesis.
messages: Cytoplasm

224308:BSU 12000
gene id: 224308:BSU_12000
uniprot id: O31644
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Cells lacking this gene are unable to grow with mannose as the sole carbon source.
messages: Positively regulates the expression of the mannose operon that consists of three genes, manP, manA, and yjdF, which are responsible for the transport and utilization of mannose. Also activates its own expression.
messages: The regulatory activity of ManR is modulated by phosphorylation and dephosphorylation of the various ManR domains. It becomes activated via phosphoryl group transfer from PEP, EI and HPr on the two conserved histidine residues in the PRD 2 domain, whereas phosphorylation of the EIIA-like domain on His-570 by the PTS EIIB-Man domain of ManP inactivates ManR (PubMed:23551403).
messages: Up-regulated by mannose. Is subject to carbon catabolite repression (CCR) by glucose.

224308:BSU 13830
gene id: 224308:BSU_13830
uniprot id: A0A6M3ZAN8
uniprot id: O31686
names: Miscellaneous
names: Subcellular Location Annotation
messages: Forespore membrane
messages: Membrane
messages: YkvU and stoA are cotranscribed under the control of sigma E.

224308:BSU 25930
gene id: 224308:BSU_25930
uniprot id: P45940
names: Similarity
messages: To B.subtilis XkdX.

224308:BSU 24890
gene id: 224308:BSU_24890
uniprot id: A0A6M4JKS4
uniprot id: P54491
names: Similarity
messages: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.

224308:BSU 11140
gene id: 224308:BSU_11140
uniprot id: P70947
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.
messages: Catalyzes the dephosphorylation of the riboflavin precursor 5-amino-6-(5-phospho-D-ribitylamino)uracil and of flavin mononucleotide (FMN) in vitro (PubMed:26316208).

224308:BSU 33120
gene id: 224308:BSU_33120
uniprot id: A0A6M4JP31
uniprot id: O32201
names: Induction
names: Similarity
messages: Belongs to the PspA/IM30 family.
messages: Induced, via the two-component regulatory system LiaS/LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.

224308:BSU 36460
gene id: 224308:BSU_36460
uniprot id: P94576
names: Subcellular Location Annotation
messages: Secreted

224308:BSU 12640
gene id: 224308:BSU_12640
uniprot id: P54330
names: Similarity
messages: To B.subtilis YqbJ.

224308:BSU 32590
gene id: 224308:BSU_32590
uniprot id: A0A6M4JP09
uniprot id: O32155
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Probably part of the binding-protein-dependent transport system YurMNO. Probably responsible for the translocation of the substrate across the membrane.

224308:BSU 19610
gene id: 224308:BSU_19610
uniprot id: O34654
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 28610
gene id: 224308:BSU_28610
uniprot id: A0A6M4JLR1
uniprot id: P94542
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activator of cell division through the inhibition of FtsZ GTPase activity, therefore promoting FtsZ assembly into bundles of protofilaments necessary for the formation of the division Z ring. It is recruited early at mid-cell but it is not essential for cell division (By similarity).
messages: Activator of cell division through the inhibition of FtsZ GTPase activity, therefore promoting FtsZ assembly into bundles of protofilaments necessary for the formation of the division Z ring. It is recruited early at mid-cell but it is not essential for cell division.
messages: Belongs to the ZapA family. Type 2 subfamily.
messages: Cytoplasm
messages: Homodimer. Interacts with FtsZ (By similarity).
messages: Homodimer. Interacts with FtsZ.

224308:BSU 08400
gene id: 224308:BSU_08400
uniprot id: A0A6M3Z8P0
uniprot id: O31563
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 18790
gene id: 224308:BSU_18790
uniprot id: O34947
names: Similarity
messages: Belongs to the peptidase C56 family.

224308:BSU 23010
gene id: 224308:BSU_23010
uniprot id: P50730
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 23210
gene id: 224308:BSU_23210
uniprot id: A0A6M4JNC5
uniprot id: P35155
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ScpB family.
messages: Cytoplasm
messages: Homodimer. Homodimerization may be required to stabilize the binding of ScpA to the Smc head domains. Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.
messages: Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves.

224308:BSU 16990
gene id: 224308:BSU_16990
uniprot id: A0A6M3ZBA2
uniprot id: O31776
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.
messages: Cytoplasm
messages: Homotetramer.

224308:BSU 14030
gene id: 224308:BSU_14030
uniprot id: A0A6M3ZAE5
uniprot id: O31695
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 26810
gene id: 224308:BSU_26810
uniprot id: A0A6M4JJM7
uniprot id: O05412
names: Function
names: Similarity
messages: Belongs to the aspartate/glutamate racemases family.
messages: Provides the (R)-glutamate required for cell wall biosynthesis.

224308:BSU 24340
gene id: 224308:BSU_24340
uniprot id: A0A6M3ZE38
uniprot id: P49787
names: Caution
names: Cofactor
names: Function
names: Subunit
messages: Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.
messages: Binds 2 magnesium or manganese ions per subunit.
messages: Leu-235 is present instead of the conserved His which is expected to bind ATP.
messages: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.

224308:BSU 38830
gene id: 224308:BSU_38830
uniprot id: A0A6M3ZHV1
uniprot id: P94358
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Activated by SigB in response to stress due to ethanol.
messages: Belongs to the aldehyde dehydrogenase family.
messages: May contribute to protect cells against stress due to ethanol and related compounds.
messages: No effect on vanillin degradation.

224308:BSU 09400
gene id: 224308:BSU_09400
uniprot id: P37875
names: Function
names: Similarity
messages: Appears to be involved in spore cortex formation.
messages: To E.coli YcgB.

224308:BSU 05140
gene id: 224308:BSU_05140
uniprot id: O31493
names: Caution
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane
messages: Could be the product of a pseudogene. The N-terminus is about 275 residues shorter than orthologs.

224308:BSU 09880
gene id: 224308:BSU_09880
uniprot id: A0A6M3Z9J1
uniprot id: O07533
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the enoyl-CoA hydratase/isomerase family.
messages: Cell membrane

224308:BSU 40160
gene id: 224308:BSU_40160
uniprot id: Q45594
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M50B family.
messages: Binds 1 zinc ion per subunit.
messages: Cell membrane
messages: Required for production of the modified peptide YydF (Probable). May process the precursor form of YydF to release the active peptide (Potential).
messages: Transcriptionally repressed by rok.

224308:BSU 40140
gene id: 224308:BSU_40140
uniprot id: Q45592
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Suggested to be part of an ABC transporter complex YydIJ involved in export of the modified peptide YydF.
messages: The complex is composed of 2 ATP-binding proteins (YydI), two transmembrane proteins (YydJ).
messages: Transcriptionally repressed by rok (PubMed:15743949), this was not found to be the case in another study (PubMed:17921301).
messages: Up-regulates expression of the LiaRS two-component regulatory system; this effect is more pronounced on modified competence medium than on rich or sporulation medium.

224308:BSU 29710
gene id: 224308:BSU_29710
uniprot id: A0A6M3ZIG1
uniprot id: P39067
names: Function
names: Similarity
messages: Belongs to the histone deacetylase family.
messages: Role in growth and sporulation on acetoin or butanediol. Involved in the breakdown of these compounds used as a carbon source.

224308:BSU 06480
gene id: 224308:BSU_06480
uniprot id: A0A6M3Z8J7
uniprot id: P12042
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FGAMS family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
messages: Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

224308:BSU 29380
gene id: 224308:BSU_29380
uniprot id: A0A6M3ZF81
uniprot id: O34406
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.
messages: The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).

224308:BSU 40170
gene id: 224308:BSU_40170
uniprot id: Q45595
names: Cofactor
names: Function
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Required for production of the modified peptide YydF (Probable). May activate a metalloenzyme (Potential).

224308:BSU 38780
gene id: 224308:BSU_38780
uniprot id: P94362
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M48 family.
messages: Binds 1 zinc ion per subunit.
messages: Cell membrane

224308:BSU 07370
gene id: 224308:BSU_07370
uniprot id: O06476
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 38500
gene id: 224308:BSU_38500
uniprot id: A0A6M4JQH3
uniprot id: P39581
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.
messages: Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 02780
gene id: 224308:BSU_02780
uniprot id: O34538
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane raft

224308:BSU 35430
gene id: 224308:BSU_35430
uniprot id: A0A6M4JPQ3
uniprot id: P39809
names: Function
names: Similarity
messages: Allows the coupling of early and late flagellar synthesis through the repression of RNA polymerase sigma-D factor-dependent transcription.
messages: Belongs to the FlgM family.

224308:BSU 20890
gene id: 224308:BSU_20890
uniprot id: O31930
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 08440
gene id: 224308:BSU_08440
uniprot id: O31567
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Cytoplasm
messages: Induced by iron starvation, repressed by fur.
messages: Membrane raft
messages: Part of the ABC transporter complex YfiYZ/YfhA/YusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of YfiZ/YfhA (Probable).
messages: Strains lacking this gene show a reduction in growth stimulation by the iron-hydroxamate siderophores schizokinen and arthrobactin compared to wild-type.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (YfiZ and YfhA) and a solute-binding protein (YfiY). Interacts with FloT (PubMed:23651456).

224308:BSU 11720
gene id: 224308:BSU_11720
uniprot id: A0A6M4JFB2
uniprot id: P54616
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.
messages: By cold shock.
messages: Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism.
messages: Homotetramer.
messages: Inhibited by triclosan and acrylamide.

224308:BSU 19590
gene id: 224308:BSU_19590
uniprot id: A0A6M4JJ69
uniprot id: O34666
names: Disruption Phenotype
names: Induction
names: Similarity
messages: Belongs to the peptidase S41A family.
messages: Is expressed only during vegetative growth.
messages: Sporulation is not significantly affected.

224308:BSU 35530
gene id: 224308:BSU_35530
uniprot id: A0A6M4JPR3
uniprot id: O34753
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 4 family.
messages: Catalyzes the formation of undecaprenyl-PP-N-acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to the peptidoglycan of B.subtilis; may also be involved in teichuronic acid biosynthesis (Probable).
messages: Cell membrane
messages: Membrane

224308:BSU 01160
gene id: 224308:BSU_01160
uniprot id: A0A6M3Z778
uniprot id: P42920
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL3 family.
messages: One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit (By similarity). Strongly stimulates 23S rRNA precursor processing by mini-ribonuclease 3 (MrnC); 20-30% DMSO can replace L3, suggesting the protein may alter rRNA conformation.
messages: One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Forms a cluster with proteins L14 and L19 (By similarity). Interacts with RNA helicase CshA (PubMed:23175651).
messages: Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.

224308:BSU 11900
gene id: 224308:BSU_11900
uniprot id: O31634
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02440
gene id: 224308:BSU_02440
uniprot id: P40758
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homotrimer (PubMed:17001076, PubMed:25691471). Under poor nitrogen source such as nitrate, the complex between GlnK and AmtB, which are the transmembrane ammonium transporter and its cognate regulator, respectively, interacts with TnrA (PubMed:17001076, PubMed:21435182, PubMed:25691471). GlnK-ATP complex are not able to bind TnrA (PubMed:21435182).
messages: In the absence of nitrogen source, cells lacking this gene do not show degradation of TnrA, which is entirely soluble.
messages: Member of the two-component regulatory system GlnK/GlnL that positively regulates the expression of the glsA-glnT operon in response to glutamine (PubMed:11717295, PubMed:15995196). It seems that autophosphorylated GlnK transfers a phosphoryl group to GlnL, which positively regulates the expression of the glsA-glnT operon (PubMed:11717295, PubMed:15995196). Interaction between GlnK-AmtB complex and TnrA protects TnrA from proteolytic degradation (PubMed:17001076, PubMed:21435182).

224308:BSU 34800
gene id: 224308:BSU_34800
uniprot id: A0A6M3ZHC2
uniprot id: P40767
names: Activity Regulation
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Detected in exponentially growing cells, the 50 and 30 kDa processing products disappear upon entry into stationary phase with the concomitant appearance of a 20 kDa products. The 50 kDa form persists in the absence of extracellular proteases (PubMed:11987133).
messages: Disruption of the cwlO gene affects neither the vegetative cell growth rate, cell morphology, motility, sporulation frequency nor the germination frequency.
messages: Expressed during the early stage of the vegetative growth phase (PubMed:16233686). Not detected in stationary phase (PubMed:11987133).
messages: Identified in the extracellular proteome as a number of processing products of about 50 and 30 kDa.
messages: Positively regulated by the two-component system YycFG.
messages: Secreted
messages: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity.
messages: The N-terminal part of CwlO was shown to have no cell wall-binding activity.
messages: cell wall

224308:BSU 32350
gene id: 224308:BSU_32350
uniprot id: O32131
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression controlled by a sigma-E-regulated promoter which needs the sigma-E factor for the binding of the RNA polymerase and subsequent transcription.
messages: Required for sporulation.

224308:BSU 16720
gene id: 224308:BSU_16720
uniprot id: Q04811
names: Similarity
messages: Belongs to the YlmC/YmxH family.

224308:BSU 33640
gene id: 224308:BSU_33640
uniprot id: O32234
names: Induction
names: Similarity
messages: Belongs to the AB hydrolase superfamily.
messages: Repressed by RghR.

224308:BSU 02730
gene id: 224308:BSU_02730
uniprot id: P70954
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system NatK/NatR that positively regulates the expression of the natAB operon. Potentially phosphorylates NatR.

224308:BSU 00180
gene id: 224308:BSU_00180
uniprot id: A0A6M3Z6C2
uniprot id: P21335
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the cytidine and deoxycytidylate deaminase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).
messages: Homodimer.

224308:BSU 31270
gene id: 224308:BSU_31270
uniprot id: A0A6M4JMD6
uniprot id: P40746
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the bacillus TGase family.
messages: Expressed during sporulation.
messages: In cells missing this gene some GerQ multimers are present, indicating that tgl is not essential. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated yabG-deleted spores but not in tgl/yabG double-mutant spores. Additionally, the germination frequency of the tgl/yabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores.
messages: Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links.
messages: Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.

224308:BSU 23920
gene id: 224308:BSU_23920
uniprot id: P54541
names: Similarity
names: Subunit
messages: Belongs to the AccD/PCCB family.
messages: Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits.

224308:BSU 30380
gene id: 224308:BSU_30380
uniprot id: A0A6M4JL34
uniprot id: O34697
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Expression is induced by bacitracin, via the two-component regulatory system BceS/BceR.
messages: Part of the ABC transporter complex BceAB (TC 3.A.1.123.5) involved in bacitracin export. Responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (BceA) and two transmembrane proteins (BceB).

224308:BSU 13930
gene id: 224308:BSU_13930
uniprot id: P37956
names: Cofactor
names: Developmental Stage
names: Function
names: Similarity
names: Subunit
messages: Belongs to the radical SAM superfamily. SPL family.
messages: Binds 1 S-adenosyl-L-methionine per subunit.
messages: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Expressed at stage III of sporulation in the forespore compartment of the developing sporangium.
messages: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6-dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines.
messages: Monomer or homodimer.

224308:BSU 15820
gene id: 224308:BSU_15820
uniprot id: A0A6M4JLS1
uniprot id: P37807
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL28 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 00480
gene id: 224308:BSU_00480
uniprot id: A0A6M3Z6G0
uniprot id: P37552
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities. Involved in the isoleucine biosynthesis.
messages: Belongs to the RutC family.
messages: Cytoplasm
messages: Homotrimer.
messages: Was originally (PubMed:10368157) thought to have a role in the purine repressor-mediated regulation of purA or the pur operon. The effect of yabJ (now ridA) on regulation of purA was due to the orientation of the marker gene downstream of purR in the yabJ mutant strain used in PubMed:10368157.

224308:BSU 18530
gene id: 224308:BSU_18530
uniprot id: O34569
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 06160
gene id: 224308:BSU_06160
uniprot id: A0A6M3ZJA7
uniprot id: O34368
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:galactoside symporter (TC 2.A.2) family.
messages: Cell membrane
messages: Membrane
messages: Probably involved in glucitol uptake.

224308:BSU 00380
gene id: 224308:BSU_00380
uniprot id: A0A6M3Z6D7
uniprot id: P37465
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
messages: Cytoplasm
messages: Homodimer.
messages: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 35070
gene id: 224308:BSU_35070
uniprot id: A0A6M3ZGP1
uniprot id: O34351
names: Function
names: Similarity
names: Subunit
messages: Belongs to the CDPS family.
messages: Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. It uses activated amino acids in the form of aminoacyl-tRNAs (aa-tRNAs) as substrates to catalyze the ATP-independent formation of cyclodipeptides which are intermediates in diketopiperazine (DKP) biosynthetic pathways. Catalyzes the formation of cyclo(L-Leu-L-Leu) (cLL) from L-leucyl-tRNA(Leu). Can also incorporate various nonpolar residues, such as L-phenylalanine, L-leucine and methionine, into cyclodipeptides.
messages: Monomer.

224308:BSU 02640
gene id: 224308:BSU_02640
uniprot id: A0A6M4JJQ6
uniprot id: P42252
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis possesses two minimal, substrate-specific, Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a TatA and a TatC protein. TatA is bifunctional and performs the function of both the TatA and TatB proteins of Gram-negative organisms.
messages: Belongs to the TatC family.
messages: Cell membrane
messages: Depletion of TatCd results in a drastic reduction of TatAd.
messages: Expressed under conditions of phosphate starvation.
messages: Forms a complex with TatA.
messages: Forms a complex with TatAd. Two types of complexes exist: one composed of TatAd and TatCd, and another composed only of TatAd.
messages: Membrane
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Required for PhoD secretion. TatCd promotes membrane localization of TatAd via domain specific interactions. TatCd is required for stabile production of TatAd as well as for its maintenance.

224308:BSU 13000
gene id: 224308:BSU_13000
uniprot id: A0A6M4JHB4
uniprot id: C0SP98
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 15260
gene id: 224308:BSU_15260
uniprot id: A0A6M3ZAK1
uniprot id: Q45544
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0749 family.
messages: Cell membrane

224308:BSU 01820
gene id: 224308:BSU_01820
uniprot id: P19220
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the MGMT family.
messages: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
messages: This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
messages: Up-regulated by methylated AdaA in response to the exposure to alkylating agents such as MNNG.

224308:BSU 16410
gene id: 224308:BSU_16410
uniprot id: P40742
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ParA family.
messages: Interacts (via N-terminal region) with GTP-bound FlhF homodimer (via NG domain).
messages: Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.

224308:BSU 12060
gene id: 224308:BSU_12060
uniprot id: A0A6M4JH57
uniprot id: O31650
names: Similarity
messages: Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily.

224308:BSU 03950
gene id: 224308:BSU_03950
uniprot id: A0A6M3ZID9
uniprot id: C0SP95
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells lacking this gene show a growth-defective phenotype under copper deprivation as well as a reduced intracellular content of copper.
messages: Highly induced under copper-limiting conditions. Down-regulated by YcnK, especially under high copper concentrations. Down-regulated by CsoR.
messages: In the C-terminal section; belongs to the CopD family.
messages: In the N-terminal section; belongs to the CopC family.
messages: Involved in uptake of extracellular oxidized copper under copper-limiting conditions.
messages: Membrane

224308:BSU 12820
gene id: 224308:BSU_12820
uniprot id: O34800
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Antitoxin component of a type II toxin-antitoxin (TA) system. Antitoxin that binds cognate toxin SpoIISA and neutralizes its toxic activity; unlike most antitoxins it does not seem to be highly labile upon expression in E.coli.
messages: Belongs to the SpoIISB antitoxin family.
messages: Decreases sporulation efficiency by 4 orders of magnitude. A double spoIISA-spoIISB disruption sporulates normally, suggesting its only role is to neutralize SpoIISA.
messages: Expressed during exponential growth and sporulation. A member of the spoIISA-spoIISB operon, it also has its own promoter.
messages: The isolated protein is unfolded; X-ray data suggests the inactive complex forms a heterotetramer of SpoIISA(2)-SpoIISB(2), which inactivates the toxic activity of SpoIISA.

224308:BSU 17190
gene id: 224308:BSU_17190
uniprot id: A0A6M3ZBC5
uniprot id: Q05470
names: Cofactor
names: Function
names: Subcellular Location Annotation
messages: Binds 5 phosphopantetheines covalently.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 29860
gene id: 224308:BSU_29860
uniprot id: A0A6M4JNR1
uniprot id: O34924
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M42 family.
messages: Binds 2 divalent metal cations per subunit.

224308:BSU 03520
gene id: 224308:BSU_03520
uniprot id: A0A6M3ZAH3
uniprot id: Q08788
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioesterase family.
messages: Cytoplasm
messages: In response to low temperature.
messages: Probable thioesterase involved in the biosynthesis of surfactin.

224308:BSU 24740
gene id: 224308:BSU_24740
uniprot id: A0A6M3ZDS3
uniprot id: P40948
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.
messages: By secretion stress.
messages: Cell membrane
messages: Homopentamer. In the absence of Mg(2+), interactions between subunits are weakened, and dimers, trimers and tetramers can be observed in vitro (By similarity). Homotetramer (PubMed:15231793).
messages: Mediates influx of magnesium ions. Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high.
messages: Membrane
messages: The central ion permeation pathway is formed by the first transmembrane domain from each of the five subunits. Mg(2+) binding strengthens interactions between subunits and leads to the formation of a symmetrical homopentamer surrounding a closed ion permeation pathway. Low Mg(2+) concentrations trigger both a conformation change within each subunit and a loosening of the interactions between subunits. This results in an open ion conduction pathway. In addition, this results in a less symmetrical shape of the whole complex.

224308:BSU 35190
gene id: 224308:BSU_35190
uniprot id: O34796
names: Similarity
messages: Belongs to the PEP-utilizing enzyme family.

224308:BSU 25090
gene id: 224308:BSU_25090
uniprot id: A0A6M3ZDS0
uniprot id: P54480
names: Similarity
messages: Belongs to the 5'(3')-deoxyribonucleotidase family.

224308:BSU 24990
gene id: 224308:BSU_24990
uniprot id: A0A6M3ZDR0
uniprot id: P46338
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PstS family.
messages: Cell membrane
messages: Involved in the system for phosphate transport across the cytoplasmic membrane.
messages: Membrane
messages: Part of the ABC transporter complex PstSACB involved in phosphate import.
messages: The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).

224308:BSU 26220
gene id: 224308:BSU_26220
uniprot id: P45948
names: Similarity
messages: To B.subtilis pcf and to sigma factors.

224308:BSU 39630
gene id: 224308:BSU_39630
uniprot id: A0A6M3ZIV6
uniprot id: P42424
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YxdLM which could be involved in peptide resistance.
messages: The complex is composed of two ATP-binding proteins (YxdL) and two transmembrane proteins (YxdM).
messages: Transcriptionally regulated by YxdJ. Induced by the antibacterial protein LL-37, probably via YxdJ.

224308:BSU 08779
gene id: 224308:BSU_08779
uniprot id: C0H3X8
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Spore coat

224308:BSU 09510
gene id: 224308:BSU_09510
uniprot id: O07581
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cotranscribed with sigma-M and yhdK. YhdL and yhdK negatively regulate sigma-M.
messages: Membrane
messages: The N-terminus of YhdL interacts with sigma-M. YhdL interacts specifically with YhdK.

224308:BSU 18430
gene id: 224308:BSU_18430
uniprot id: O35017
names: Disruption Phenotype
names: Similarity
messages: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
messages: Cells lacking this gene show up-regulated sigma X activity.

224308:BSU 26920
gene id: 224308:BSU_26920
uniprot id: O07934
names: Similarity
messages: To B.subtilis YraI.

224308:BSU 25020
gene id: 224308:BSU_25020
uniprot id: A0A6M3ZH87
uniprot id: P54375
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the iron/manganese superoxide dismutase family.
messages: Binds 1 Mn(2+) ion per subunit.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Cytoplasm
messages: Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
messages: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
messages: Homodimer.

224308:BSU 30320
gene id: 224308:BSU_30320
uniprot id: A0A6M4JLA6
uniprot id: P36430
names: Caution
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-I aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 32550
gene id: 224308:BSU_32550
uniprot id: O32151
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 00930
gene id: 224308:BSU_00930
uniprot id: A0A6M3Z6U9
uniprot id: Q06750
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transferase hexapeptide repeat family.
messages: Catalyzes the acetylation of serine by acetyl-CoA to produce O-acetylserine (OAS).
messages: Cytoplasm
messages: Inhibited by cysteine.

224308:BSU 22870
gene id: 224308:BSU_22870
uniprot id: A0A6M3ZDJ3
uniprot id: P50740
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the IPP isomerase type 2 family.
messages: Cytoplasm
messages: Homooctamer. Dimer of tetramers.
messages: Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 08210
gene id: 224308:BSU_08210
uniprot id: A0A6M4JE55
uniprot id: P54718
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 07790
gene id: 224308:BSU_07790
uniprot id: A0A6M4JDY4
uniprot id: O34486
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Membrane

224308:BSU 17810
gene id: 224308:BSU_17810
uniprot id: O31814
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 33180
gene id: 224308:BSU_33180
uniprot id: O34805
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Probably part of an ABC transporter complex.
messages: The complex is composed of two ATP-binding proteins (YvrA), two transmembrane proteins (YvrB) and a solute-binding protein (YvrC).

224308:BSU 39740
gene id: 224308:BSU_39740
uniprot id: A0A6M4JQV7
uniprot id: P42414
names: Function
names: Similarity
messages: Belongs to the carbohydrate kinase PfkB family.
messages: Catalyzes the phosphorylation of 5-dehydro-2-deoxy-D-gluconate (2-deoxy-5-keto-D-gluconate or DKG) to 6-phospho-5-dehydro-2-deoxy-D-gluconate (DKGP).

224308:BSU 19080
gene id: 224308:BSU_19080
uniprot id: O34910
names: Cofactor
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 12440
gene id: 224308:BSU_12440
uniprot id: Q00829
names: Activity Regulation
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Phr family.
messages: Cytoplasm
messages: Inhibition of RapA requires a free carboxylate group at the C-terminal end of the PhrA pentapeptide (PubMed:11587784). A free C-terminal carboxylic acid PhrA pentapeptide inhibits RapA phosphatase activity at a 1:1 ratio and is approximately 200 fold more active than a C-terminal amide peptide (PubMed:11587784).
messages: Interacts with RapA and inhibits its interaction with Spo0F.
messages: Secreted
messages: Secreted with a propeptide domain, which is cleaved in the cell wall by the secreted serine proteases subtilisin and Vpr to produce a mature signaling peptide (PubMed:17666034). Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably subject to only one processing event, at the N-terminal end of the mature peptide (Probable).
messages: Signaling molecule involved in the regulation of sporulation (PubMed:8643670, PubMed:9238025). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (PubMed:9200610, PubMed:8643670, PubMed:9238025). Inhibitor of the RapA phosphatase activity (PubMed:8643670, PubMed:9238025, PubMed:11923303, PubMed:22267516). Does not act on RapB (PubMed:9238025).

224308:BSU 25550
gene id: 224308:BSU_25550
uniprot id: A0A6M3ZE90
uniprot id: P21477
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bS20 family.
messages: Binds directly to 16S ribosomal RNA.
messages: Part of the 30S ribosomal subunit.

224308:BSU 36580
gene id: 224308:BSU_36580
uniprot id: P71041
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 08880
gene id: 224308:BSU_08880
uniprot id: A0A6M3ZE09
uniprot id: O31587
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS14 family.
messages: Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.
messages: Contacts proteins S3 and S10 (By similarity). Part of the 30S ribosomal subunit.
messages: Non-essential protein. A second form of uS14, it can integrate into the 30S subunit where it partially compensates for loss of the major uS14 protein (AC P12878) in restoring 70S formation, although it does not seem to be incorporated into the ribosome as well as the major uS14.
messages: Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.
messages: Repressed by the zinc-specific metallo-regulatory protein zur.

224308:BSU 17690
gene id: 224308:BSU_17690
uniprot id: O31803
names: Similarity
names: Subcellular Location Annotation
messages: Secreted
messages: To B.subtilis YrpD.

224308:BSU 27650
gene id: 224308:BSU_27650
uniprot id: A0A6M4JNL2
uniprot id: O32047
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SecD/SecF family. SecD subfamily.
messages: Belongs to the SecD/SecF family. SecF subfamily.
messages: Cell membrane
messages: Cells lacking this gene show a cold-sensitive phenotype and a filamentous morphology.
messages: Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.
messages: Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.
messages: In the C-terminal section; belongs to the SecD/SecF family. SecF subfamily.
messages: In the N-terminal section; belongs to the SecD/SecF family. SecD subfamily.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA (By similarity).
messages: Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.
messages: Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity).
messages: Required for efficient translocation of secretory pre-proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane.
messages: Shows maximal expression at the beginning of post-exponential growth phase and increased expression by glucose in the post-exponential growth phase when cells are cultured on rich medium. Expressed constitutively during growth in minimal medium.

224308:BSU 04120
gene id: 224308:BSU_04120
uniprot id: P42970
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 23110
gene id: 224308:BSU_23110
uniprot id: A0A6M4JNC2
uniprot id: P35164
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Member of the two-component regulatory system ResD/ResE involved in the global regulation of aerobic and anaerobic respiration. Probably phosphorylates ResD.
messages: Membrane
messages: Membrane raft
messages: Oligomerizes, probably forms homodimers. Interacts in vivo with FloT, colocalizes with FloT-only membrane rafts. Oligomerization is assisted by FloT (PubMed:26297017, PubMed:25909364). Another study shows only minor colocalization with FloT membrane assemblies (PubMed:27362352).

224308:BSU 14480
gene id: 224308:BSU_14480
uniprot id: P39758
names: Similarity
messages: To B.subtilis AbrB and SpoVT.

224308:BSU 39790
gene id: 224308:BSU_39790
uniprot id: P46335
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29580
gene id: 224308:BSU_29580
uniprot id: A0A6M3ZFA6
uniprot id: O34595
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ThiI family.
messages: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
messages: Cytoplasm

224308:BSU 03660
gene id: 224308:BSU_03660
uniprot id: P94407
names: Similarity
messages: Belongs to the AB hydrolase superfamily.

224308:BSU 04630
gene id: 224308:BSU_04630
uniprot id: P96619
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is controlled by a sigma-E-regulated promoter which needs the sigma-E factor for the binding of the RNA polymerase and subsequent transcription.
messages: required for efficient sporulation.

224308:BSU 08260
gene id: 224308:BSU_08260
uniprot id: A0A6M4JHI3
uniprot id: P54723
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 15740
gene id: 224308:BSU_15740
uniprot id: A0A6M3ZAM9
uniprot id: P94464
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.
messages: Cytoplasm
messages: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.

224308:BSU 29220
gene id: 224308:BSU_29220
uniprot id: A0A6M4JMD9
uniprot id: O34962
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the malic enzymes family.
messages: Bifunctional enzyme with both malic and malolactic enzyme activities (PubMed:33824210). In the absence of NADPH, catalyzes the reversible decarboxylation of malate to pyruvate (PubMed:16788182, PubMed:33824210). Can use NAD and NADP, but with a very strong preference for NADP (PubMed:16788182). In the presence of excess NADPH, catalyzes the non-oxidative decarboxylation of malate to lactate (PubMed:33824210). During growth on glucose, contributes to NADPH balancing via oxidation of the NADPH produced in excess by other enzymatic reactions (PubMed:33824210). Can also catalyze the decarboxylation of oxaloacetate (PubMed:16788182).
messages: Divalent metal cations.
messages: Divalent metal cations. Prefers magnesium or manganese.
messages: Interacts with BrxC.
messages: Mutant shows wild-type growth on glucose but a much slower growth rate on malate, fumarate, or succinate plus glutamate (PubMed:16788182). Overexpression of the other three malic enzymes, mleA, maeA or malS cannot compensate for the ytsJ deletion (PubMed:16788182). The ATP concentrations in the mutant grown in minimal medium with glucose are similar to the wild-type level. ATP concentrations decrease by about 20% in malate minimal medium (PubMed:23136871). NADPH overproduction is virtually abolished in the deletion mutant (PubMed:33824210).
messages: NADPH is a strong modulator that switches activity from a pyruvate-producing malic enzyme to a lactate-generating malolactic enzyme.
messages: Transcribed at a high level under all of the conditions tested.

224308:BSU 18210
gene id: 224308:BSU_18210
uniprot id: O31825
names: Similarity
messages: Belongs to the AccD/PCCB family.

224308:BSU 20070
gene id: 224308:BSU_20070
uniprot id: A0A6M3ZCR6
uniprot id: P68524
names: Function
names: Similarity
messages: Belongs to the NrdI family.
messages: Probably involved in ribonucleotide reductase function.

224308:BSU 01070
gene id: 224308:BSU_01070
uniprot id: A0A6M3Z6N2
uniprot id: P37870
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RNA polymerase beta chain family.
messages: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

224308:BSU 19230
gene id: 224308:BSU_19230
uniprot id: A0A6M4JKS8
uniprot id: O35022
names: Caution
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.
messages: Belongs to the azoreductase type 1 family.
messages: Binds 1 FMN per subunit.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones (Probable). Contributes to resistance to 2-methylhydroquinone (2-MHQ) and catechol (PubMed:18208493).
messages: Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.
messages: Repressed by YodB. Induced by thiol specific stress conditions, such as exposure to 2-methylhydroquinone (2-MHQ), catechol or diamide. Not induced by oxidative stress due to hydrogen peroxide.

224308:BSU 26910
gene id: 224308:BSU_26910
uniprot id: O07937
names: Similarity
messages: Belongs to the AB hydrolase superfamily.

224308:BSU 10000
gene id: 224308:BSU_10000
uniprot id: O07516
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29470
gene id: 224308:BSU_29470
uniprot id: A0A6M3ZFG4
uniprot id: P37877
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the acetokinase family.
messages: Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.
messages: Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. Appears to favor the formation of acetate. Involved in the secretion of excess carbohydrate.
messages: Cytoplasm
messages: Homodimer.
messages: Induced by glucose excess, the induction may be mediated by CcpA transcriptional regulator.
messages: Mg(2+). Can also accept Mn(2+).

224308:BSU 28360
gene id: 224308:BSU_28360
uniprot id: A0A6M3ZEN0
uniprot id: P94558
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the HAM1 NTPase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Homodimer.
messages: Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.

224308:BSU 16870
gene id: 224308:BSU_16870
uniprot id: O31767
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 07210
gene id: 224308:BSU_07210
uniprot id: A0A6M3Z876
uniprot id: O31540
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 26090
gene id: 224308:BSU_26090
uniprot id: P45926
names: Similarity
messages: To B.subtilis XkdJ.

224308:BSU 00700
gene id: 224308:BSU_00700
uniprot id: P37564
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the type III pantothenate kinase family.
messages: Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis. Cannot utilize a phosphoryl donor other than ATP.
messages: Cytoplasm
messages: Homodimer.
messages: Monovalent cations. Ammonium or potassium.
messages: Not regulated by feedback inhibition by CoA and its thioesters as described for many other pantothenate kinases. Not inhibited by N-pentylpantothenamide (N5-Pan), and this compound cannot act as a substrate either.

224308:BSU 17699
gene id: 224308:BSU_17699
uniprot id: C0H417
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32240
gene id: 224308:BSU_32240
uniprot id: A0A6M4JN39
uniprot id: P04948
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GHMP kinase family. Homoserine kinase subfamily.
messages: Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.
messages: Cytoplasm

224308:BSU 06570
gene id: 224308:BSU_06570
uniprot id: O34968
names: Disruption Phenotype
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Interacts with PcrA. The interaction is not essential for cell viability or repair of UV-induced lesions.
messages: No PcrA-related phenotype.

224308:BSU 02460
gene id: 224308:BSU_02460
uniprot id: A0A6M4JI46
uniprot id: P42235
names: Similarity
messages: Belongs to the DapA family.

224308:BSU 12590
gene id: 224308:BSU_12590
uniprot id: A0A6M3Z9S2
uniprot id: P54325
names: Similarity
messages: Belongs to the phage portal family. PBSX subfamily.

224308:BSU 02190
gene id: 224308:BSU_02190
uniprot id: O31446
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31480
gene id: 224308:BSU_31480
uniprot id: A0A6M3ZFS2
uniprot id: P40760
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 24120
gene id: 224308:BSU_24120
uniprot id: A0A6M3ZE80
uniprot id: P54528
names: Function
names: Similarity
messages: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.
messages: Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.
messages: Involved in the methylcitric acid cycle. Catalyzes the cleavage of 2-methylisocitrate to yield pyruvate and succinate.

224308:BSU 35520
gene id: 224308:BSU_35520
uniprot id: A0A6M4JPN8
uniprot id: P96499
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LytR/CpsA/Psr (LCP) family.
messages: Cell membrane
messages: May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).
messages: Single mutant has no effect on cell growth or morphology under normal growth conditions. Triple disruption of tagTUV genes is not viable.

224308:BSU 05190
gene id: 224308:BSU_05190
uniprot id: A0A6M3ZIT7
uniprot id: P96664
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 12890
gene id: 224308:BSU_12890
uniprot id: O34319
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 2 family. GtrB subfamily.
messages: Cell membrane

224308:BSU 13820
gene id: 224308:BSU_13820
uniprot id: O31685
names: Induction
messages: Positively regulated by WalR. Expressed mainly during exponential growth and rapidly shut off as cells enter the stationary phase.

224308:BSU 25410
gene id: 224308:BSU_25410
uniprot id: A0A6M4JKW8
uniprot id: P21478
names: Similarity
messages: Belongs to the bacterial ribosomal protein bS21 family.

224308:BSU 15110
gene id: 224308:BSU_15110
uniprot id: A0A6M4JG43
uniprot id: O34661
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ketopantoate reductase family.
messages: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
messages: Cytoplasm

224308:BSU 01090
gene id: 224308:BSU_01090
uniprot id: A0A6M4JJB4
uniprot id: P46350
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the eukaryotic ribosomal protein eL8 family.
messages: Cytoplasm
messages: Found associated with the 50S subunit of the ribosome during exponential growth but not during stationary phase.
messages: None; has no effect on viability, growth or sporulation at low, normal or elevated temperatures.
messages: RNA-binding protein that recognizes the K-turn motif present in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.

224308:BSU 15420
gene id: 224308:BSU_15420
uniprot id: A0A6M4JG33
uniprot id: P71021
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DivIVA family.
messages: Constitutively expressed.
messages: Cytoplasm
messages: May act as a pilot protein, directing MinCD to the polar septation sites or by inhibiting MinCD at the midcell site of division. Required for polar localization of the chromosome during sporulation.
messages: Misplacement of the septum during cell division, resulting in the formation of small, circular, anucleate minicells.
messages: Oligomers. Interacts with FtsZ, MinD, MinJ and Spo0J. The association with Spo0J is transient and occurs at a specific point during development.
messages: Overexpression of DivIVA is lethal.

224308:BSU 06960
gene id: 224308:BSU_06960
uniprot id: O31517
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YesM/YesN.
messages: Phosphorylated by YesM.

224308:BSU 03170
gene id: 224308:BSU_03170
uniprot id: A0A6M4JCM5
uniprot id: P94387
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 39060
gene id: 224308:BSU_39060
uniprot id: A0A6M3ZI09
uniprot id: P42308
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CitM (TC 2.A.11) transporter family.
messages: Cell membrane
messages: Membrane
messages: Transports the free citrate anion. Probably cotransports citrate and at least three or four protons. The citrate uptake is inhibited by the presence of magnesium ions.

224308:BSU 09370
gene id: 224308:BSU_09370
uniprot id: A0A6M3Z8W6
uniprot id: O07532
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Cell wall hydrolase that cleaves gamma-D-glutamate-meso-diaminopimelate bonds in peptidoglycan. LytF is necessary and sufficient for vegetative daughter cell separation, and also seems to play a role in cell autolysis.
messages: Cells from an undomesticated strain (3610) lacking this gene grow predominantly as chains, whereas wild-type cells grow as separate individuals. They do not show a reduction in the rate of swarming motility.
messages: Expressed in the vegetative growth phase under exclusive control of sigma-D (SigD). Expression of lytF is heterogeneous in the exponentially growing cell population; it is ON in single cells and OFF in long chains. The same subpopulation of cells that express lytF also express flagellin.
messages: In domesticated laboratory strains (168 and derivatives), lytF is not expressed in a majority of the growing cells. These cells form a subpopulation that grows in multicellular, nonmotile chains. Undomesticated strains (3610) express lytF in a majority of the population, that grows as separate individual motile cells (PubMed:19542270).
messages: Is inhibited in vitro by para-hydroxymercuribenzoate, a sulfydryl inhibitor.
messages: The N-terminal domain contains LysM domains that are thought to be involved in peptidoglycan binding, while the C-terminal domain is endowed with the catalytic activity.
messages: cell wall

224308:BSU 33000
gene id: 224308:BSU_33000
uniprot id: A0A6M3ZGH7
uniprot id: Q9R9I1
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S1C family.
messages: Cell membrane
messages: Degrades abnormal exported proteins and responsible for the propeptide processing of a natural pro-protein and for the maturation of a native protein. It also plays a prominent role in stress (heat shock, ethanol, puromycin and NaCl) resistance during active exponential growth (Probable).
messages: In contrast to other bacteria, in which inactivation of serine protease leads to thermosensitivity, inactivation of HtrB leads to an increased thermotolerance and an increased tolerance to hydrogen peroxide. Inactivation of both HtrA and HtrB leads to growth defects and to thermosensitivity.
messages: Inactivation results in compensating overexpression of YkdA, especially during stress conditions.
messages: Induced by heat shock during exponential growth and by heterologous amylases at the transition phase of the growth cycle. Negatively regulates its own expression.
messages: Membrane

224308:BSU 34400
gene id: 224308:BSU_34400
uniprot id: O07006
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the PadC family.
messages: By ferulic, p-coumaric and caffeic acids (at protein level) (PubMed:9546183). Cells extracts from caffeic acid-induced cells exhibited lower activity on the three acids, which indicates that caffeic acid could be a less efficient inducer (PubMed:9546183). Up-regulated by salicylate (PubMed:17295427).
messages: Homodimer.
messages: Involved in the decarboxylation and detoxification of phenolic derivatives. It is able to catalyze the decarboxylation of ferulic, p-coumaric and caffeic acids.
messages: No longer metabolizes ferulic acid (PubMed:26658822).

224308:BSU 35570
gene id: 224308:BSU_35570
uniprot id: A0A6M4JPP8
uniprot id: O32270
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: Cell membrane
messages: Membrane
messages: Might be involved in the polymerization of teichuronic acid repeating units after their translocation to the outer surface of the membrane.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 14190
gene id: 224308:BSU_14190
uniprot id: A0A6M3ZDR9
uniprot id: O34916
names: Function
names: Similarity
messages: Belongs to the peptidase M20A family. N-acetyldiaminopimelate deacetylase subfamily.
messages: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate.

224308:BSU 12010
gene id: 224308:BSU_12010
uniprot id: A0A6M3Z9L5
uniprot id: O31645
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell inner membrane
messages: Cell membrane
messages: Cells lacking this gene are unable to grow with mannose as the sole carbon source. Deletion of manP results in constitutive expression from both the manP and manR promoters, indicating that the phosphotransferase system (PTS) component EII-Man has a negative effect on regulation of the mannose operon and manR.
messages: Membrane
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIB domain is also able to transfer its phosphoryl group to a specific histidine residue in ManR, which leads to its inactivation.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannose transport.
messages: Up-regulated by mannose. Is under the control of ManR. Is subject to carbon catabolite repression (CCR) by glucose. Forms part of an operon with manA and yjdF.

224308:BSU 26970
gene id: 224308:BSU_26970
uniprot id: A0A6M3ZEA7
uniprot id: O06012
names: Cofactor
names: Disruption Phenotype
names: Similarity
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: No aldehyde-stress related phenotype.

224308:BSU 35490
gene id: 224308:BSU_35490
uniprot id: A0A6M4JPM5
uniprot id: P13800
names: Activity Regulation
names: Domain
names: Function
names: Induction
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation, biofilm formation, and competence for DNA uptake. Positively or negatively regulates expression of many different genes. The phosphorylated form is required for synthesis of degradative enzymes, flagellum formation and biofilm formation. The unphosphorylated form is required for expression of genetic competence, via induction of comK.
messages: Phosphorylated DegU activates its own expression.
messages: Phosphorylated and dephosphorylated by DegS.
messages: Phosphorylated and unphosphorylated forms are both active and have different functions. A low concentration of phospho-DegU is sufficient to activate transcription of flagellar genes, but a higher concentration of phospho-DegU is required for transcription of other genes. Phosphorylated DegU is stabilized by DegR.
messages: The N-terminal region acts as an inhibitor, whereas the C-terminal region carries enhancing activity.

224308:BSU 04640
gene id: 224308:BSU_04640
uniprot id: A0A6M3Z7N9
uniprot id: P10725
names: Function
names: Similarity
messages: Belongs to the alanine racemase family.
messages: Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.

224308:BSU 28310
gene id: 224308:BSU_28310
uniprot id: A0A6M3ZJT0
uniprot id: P37251
names: Cofactor
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the TPP enzyme family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry (By similarity).
messages: Dimer of large and small chains.

224308:BSU 09380
gene id: 224308:BSU_09380
uniprot id: O07573
names: Cofactor
names: Function
messages: Binds 1 [2Fe-2S] cluster per subunit.
messages: Nitric oxide-responsive transcriptional regulator. It represses the expression of flavohemoprotein hmp and the nitrite reductase nasD. Probably plays a role in the up-regulation of the resDE regulon in the presence of nitric oxide.

224308:BSU 16280
gene id: 224308:BSU_16280
uniprot id: A0A6M3ZAP7
uniprot id: P23455
names: Similarity
messages: Belongs to the FlgD family.

224308:BSU 14340
gene id: 224308:BSU_14340
uniprot id: A0A6M3ZAI1
uniprot id: O31709
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Expressed in exponential-phase cells.
messages: Interacts with a complex composed of YknX, YknY and YknZ.
messages: Membrane
messages: Part of an unusual four-component transporter, which is required for protection against the killing factor SdpC (sporulation-delaying protein). Has a role in the assembly of the YknXYZ complex.

224308:BSU 27660
gene id: 224308:BSU_27660
uniprot id: O32049
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Disruption results in severely reduced transformation frequency, but has no detectable effect on either the frequency or the location of division septa.
messages: Interacts directly with DivIVA.
messages: Required for post-transcription initiation control of the comE operon. Promotes the accumulation of its target comE mRNA to septal and polar sites.

224308:BSU 02570
gene id: 224308:BSU_02570
uniprot id: P42246
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 22620
gene id: 224308:BSU_22620
uniprot id: A3F3C5
uniprot id: P17731
names: Similarity
names: Subunit
messages: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.
messages: Homodimer.

224308:BSU 26050
gene id: 224308:BSU_26050
uniprot id: P54373
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: Cell membrane
messages: Encoded in a 48-kb, phage-like element known as skin.
messages: The antisense RNA ratA acts as an antitoxin by annealing to the mRNA of txpA and causing its degradation, protecting the cell from TxpA by blocking the production of the toxin. A type I toxin-antitoxin (TA) system, where expression of the proteinaceous toxin is controlled by an antisense sRNA.
messages: Toxic component of a type I toxin-antitoxin (TA) system (PubMed:16166525). Overexpression of txpA causes cell lysis; the TxpA protein has been suggested to act on the cell membrane or might possibly block cell wall synthesis (PubMed:16166525). Overexpression in E.coli is not toxic (PubMed:16166525).

224308:BSU 05940
gene id: 224308:BSU_05940
uniprot id: A0A6M3ZB69
uniprot id: O05518
names: Caution
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the KAE1 / TsaD family.
messages: Binds 1 Fe(2+) ion per subunit.
messages: Cytoplasm
messages: May form a heterodimer with TsaB.
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB; this reaction does not require ATP in vitro. TsaD likely plays a direct catalytic role in this reaction.
messages: The four proteins YwlC, TsaD, TsaB and TsaE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine biosynthesis in vitro in B.subtilis.
messages: The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in B.subtilis and many other species (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA.

224308:BSU 17590
gene id: 224308:BSU_17590
uniprot id: A0A6M4JIM0
uniprot id: P94490
names: Function
names: Similarity
messages: Belongs to the ROK (NagC/XylR) family.
messages: Transcriptional repressor of xylose-utilizing enzymes.

224308:BSU 27620
gene id: 224308:BSU_27620
uniprot id: A0A6M3ZEJ8
uniprot id: O32044
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the RecJ family.
messages: Cells lacking this gene show only slightly increased sensitivity to DNA-damaging agents. However, cells lacking RecJ and with a mutated AadAB enzyme were extremely sensitive to these agents, as sensitive as recA deletion strains. The doubly mutated strain shows 10-fold impaired growth in the absence of these agents.
messages: Putative single-stranded-DNA-specific exonuclease (By similarity). RecA thread formation during DNA double-strand break repair requires RecJ or AadAB.

224308:BSU 13680
gene id: 224308:BSU_13680
uniprot id: A0A6M4JI07
uniprot id: P28612
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MotB family.
messages: Cell membrane
messages: Each stator complex is composed of 4 MotA and 2 MotB subunits. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels (By similarity).
messages: MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Might be a linker that fastens the torque-generating machinery to the cell wall (By similarity).

224308:BSU 14920
gene id: 224308:BSU_14920
uniprot id: A0A6M4JG21
uniprot id: P24013
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome c oxidase bacterial subunit 4 family.
messages: Cell membrane
messages: Membrane

224308:BSU 01890
gene id: 224308:BSU_01890
uniprot id: A0A6M3Z7D4
uniprot id: O34663
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 18240
gene id: 224308:BSU_18240
uniprot id: O34544
names: Cofactor
names: Function
names: Subunit
messages: Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.
messages: Binds 2 magnesium or manganese ions per subunit.
messages: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.

224308:BSU 18670
gene id: 224308:BSU_18670
uniprot id: A0A6M4JHC2
uniprot id: O34767
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Binds 2 manganese ions per subunit.
messages: Converts oxalate to formate and CO(2).
messages: Cytoplasm
messages: To B.subtilis OxdC.

224308:BSU 19580
gene id: 224308:BSU_19580
uniprot id: A0A6M4JH98
uniprot id: O34745
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
messages: Cell membrane
messages: Membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 35660
gene id: 224308:BSU_35660
uniprot id: A0A6M4JPS3
uniprot id: P39131
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UDP-N-acetylglucosamine 2-epimerase family.
messages: Catalyzes the conversion of UDP-N-acetylglucosamine into UDP-N-acetylmannosamine, a precursor of the teichoic acid linkage unit.
messages: Cytoplasm

224308:BSU 01220
gene id: 224308:BSU_01220
uniprot id: A0A6M4JC90
uniprot id: P21465
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS3 family.
messages: Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Forms a tight complex with proteins S10 and S14 (By similarity).
messages: Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.

224308:BSU 37740
gene id: 224308:BSU_37740
uniprot id: P39638
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the prephenate decarboxylase family.
messages: Cytoplasm
messages: Part of the bacABCDEF operon responsible for the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:15609023, PubMed:20052993). BacA is an unusual prephenate decarboxylase that avoids the typical aromatization of the cyclohexadienol ring of prephenate (PubMed:20052993)(PubMed:22483065). BacA catalyzes the protonation of prephenate (1-carboxy-4-hydroxy-alpha-oxo-2,5-cyclohexadiene-1-propanoic acid) at C6 position, followed by a decarboxylation to produce the endocyclic-delta(4),delta(8)-7R-dihydro-hydroxyphenylpyruvate (en-H2HPP) (PubMed:20052993, PubMed:22483065, PubMed:22765234). En-H2HPP is able to undergo a slow nonenzymatic isomerization to produce the exocyclic-delta(3),delta(5)-dihydro-hydroxyphenylpyruvate (ex-H2HPP) (PubMed:20052993)(PubMed:22483065). BacA isomerizes only the pro-R double bond in prephenate (PubMed:22483065).
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDEF operon and BacG, and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 29900
gene id: 224308:BSU_29900
uniprot id: A0A6M4JMQ2
uniprot id: O34522
names: Function
names: Similarity
names: Subunit
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
messages: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
messages: Homodimer.

224308:BSU 00400
gene id: 224308:BSU_00400
uniprot id: P37546
names: Disruption Phenotype
names: Function
names: Induction
messages: Negatively regulated by an overlapping cis-acting antisense RNA which itself is positively regulated by extracytoplasmic sigma factors SigM and SigX; when both sigma factors are deleted expression of the yabE mRNA is detected.
messages: No visible phenotype during recovery from stationary phase or sporulation efficiency.
messages: Suggested to be involved in cell wall modification.

224308:BSU 08790
gene id: 224308:BSU_08790
uniprot id: A0A6M4JEI9
uniprot id: P45740
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the ThiC family.
messages: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.
messages: Cells lacking this gene do not grow on minimal medium or on 4-methyl-5-(2-hydroxyethyl) thiazole, a thiamine precursor molecule. They do not synthesize 2-methyl-4-amino-5-hydroxymethylpyrimidine.
messages: Repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine.

224308:BSU 16470
gene id: 224308:BSU_16470
uniprot id: A0A6M4JI85
uniprot id: P10726
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Association with RNAP core increases during H(2)O(2), NaOH, rifampicin stress and during sporulation (at protein level).
messages: Belongs to the sigma-70 factor family.
messages: Monomer (PubMed:7602586). Interacts transiently with the RNAP core (Probable).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-type chemotaxis. Associates with the RNAP core during all growth phases with a peak at the transition to stationary phase (PubMed:21710567).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

224308:BSU 30900
gene id: 224308:BSU_30900
uniprot id: P46914
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotS family.
messages: Late stage of sporulation.
messages: Seems to be required for the assembly of the CotSA protein in spores.
messages: Spore coat

224308:BSU 37910
gene id: 224308:BSU_37910
uniprot id: P39621
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the glycosyltransferase 2 family.
messages: Contains an N-terminal nucleotide-binding domain and a C-terminal acceptor-binding domain.
messages: Glycosyltransferase implicated in the synthesis of the spore coat.
messages: Monomer in solution.

224308:BSU 32080
gene id: 224308:BSU_32080
uniprot id: O32109
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 11549
gene id: 224308:BSU_11549
uniprot id: C0H3Y6
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 13730
gene id: 224308:BSU_13730
uniprot id: A0A6M3ZAN2
uniprot id: O31676
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the PTPS family. QueD subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde.
messages: Homotetramer.
messages: The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit (By similarity).

224308:BSU 22860
gene id: 224308:BSU_22860
uniprot id: P50741
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 24490
gene id: 224308:BSU_24490
uniprot id: P54515
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31760
gene id: 224308:BSU_31760
uniprot id: O32091
names: Similarity
messages: Belongs to the isochorismatase family.

224308:BSU 32030
gene id: 224308:BSU_32030
uniprot id: A0A6M4JN30
uniprot id: O32104
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the BioY family.
messages: Cell membrane
messages: Putative biotin transporter.

224308:BSU 29400
gene id: 224308:BSU_29400
uniprot id: A0A6M4JMM4
uniprot id: O35038
names: Function
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.
messages: Positively regulates the expression of ytmI operon in response to the availability of sulfur sources.

224308:BSU 04310
gene id: 224308:BSU_04310
uniprot id: O31488
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 18270
gene id: 224308:BSU_18270
uniprot id: O34356
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 33160
gene id: 224308:BSU_33160
uniprot id: O34631
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Probably part of an ABC transporter complex. Probably responsible for energy coupling to the transport system (By similarity).
messages: The complex is composed of two ATP-binding proteins (YvrA), two transmembrane proteins (YvrB) and a solute-binding protein (YvrC).

224308:BSU 19120
gene id: 224308:BSU_19120
uniprot id: O31844
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
messages: Can bind copper. It does not affect DNA binding but it inhibits zinc-mediated regulation.
messages: Cytoplasm
messages: Metal-responsive transcriptional regulator that represses transcription of cadA and the czcD-trkA operon by binding specifically to their promoter. Binding of zinc causes the repressor to dissociate from the DNA.

224308:BSU 25140
gene id: 224308:BSU_25140
uniprot id: A0A6M3ZJ39
uniprot id: P54475
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DEAD box helicase family.
messages: Belongs to the DEAD box helicase family. CshB subfamily.
messages: Cytoplasm
messages: DEAD-box RNA helicase that plays a role in 70S ribosome assembly. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures.
messages: Induced by cold shock (PubMed:12399512). In rich medium highest expression in logarithmic growth, expression decreases and then disappears in stationary phase (at protein level) (PubMed:20572937, PubMed:23175651). Protein level not increased at 16 degrees Celsius. Not detected in minimal medium (at protein level) (PubMed:20572937).
messages: Interacts with CspB when cells are transcriptionally active. May interact with RNA helicases CshA and DbpA (DeaD), may be a component of a possible RNA degradosome complex composed of rny, rnja, rnjb, pnp, pfkA and eno (although rnjA and rnjB's presence is unclear). Specifically interacts with pnp and rny (PubMed:20572937).
messages: No difference in growth at 37 or 15 degrees Celsius (PubMed:16352840), decreased growth at 16 degrees Celsius (PubMed:23175651). The presence of CshA or CshB is essential for viability; in a cshA disruption mutant further depletion of cshB stops growth after 1 cell duplication (PubMed:16352840). Others show a quadruple disruption of all RNA helicases (cshA, cshB, deaD, yfmL) was not lethal at 37 degrees Celsius, although both 50S and 70S ribosomes are decreased, but growth stops at 16 degrees (PubMed:23175651). A double cshB-cspB disruption mutant cannot be made. A double cshB-cspD mutant grows slowly at 15 degrees Celsius (PubMed:16352840). Decreased amounts of 70S ribosomes (PubMed:23175651).
messages: Probable DEAD-box RNA helicase. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures.
messages: nucleoid

224308:BSU 15340
gene id: 224308:BSU_15340
uniprot id: O31723
names: Induction
names: Similarity
messages: Belongs to the ABC transporter superfamily.
messages: Highly repressed by CodY.

224308:BSU 39600
gene id: 224308:BSU_39600
uniprot id: P54942
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10700
gene id: 224308:BSU_10700
uniprot id: O06719
names: Developmental Stage
names: Function
names: Induction
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 04330
gene id: 224308:BSU_04330
uniprot id: A0A6M4JFJ5
uniprot id: P96590
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the Nudix hydrolase family.
messages: Growing cells lacking this gene have an unchanged spontaneous mutation frequency, even in triple mutT/yjhB/yvcI disruptions (PubMed:12483591), however they are more sensitive to DNA-damaging agents such as H(2)O(2) or t-BHP (PubMed:16513759). A double ytkD/mutT disruption has a greater mutation frequency than the ytkD disruption, as well as an increased sensitivity to DNA-damaging agents (PubMed:16513759). These properties are also seen in stationary phase cells (PubMed:19011023).
messages: May be involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate (By similarity). Functions, in conjunction with ytkD, to protect vegetatively growing cells from DNA-damaging agents such as H(2)O(2) or t-BHP (t-butylhydroperoxide). The 2 proteins do not however protect spores.

224308:BSU 18669
gene id: 224308:BSU_18669
uniprot id: C0H420
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29140
gene id: 224308:BSU_29140
uniprot id: A0A6M3ZJX7
uniprot id: P39120
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the citrate synthase family.
messages: By decoyinine and nutrient depletion.
messages: Citrate synthase is found in nearly all cells capable of oxidative metabolism.
messages: Homodimer.
messages: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect.

224308:BSU 31910
gene id: 224308:BSU_31910
uniprot id: A0A6M4JMN8
uniprot id: C0SP85
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the WXG100 family.
messages: Belongs to the WXG100 family. sagEsxA-like subfamily.
messages: Cells lacking this gene display an increased bacteriophage SPP1 resistance phenotype (PubMed:15576783). Loss of delivery of LXG type toxins to target cells (PubMed:34280190).
messages: Homodimer.
messages: Positively regulated by phosphorylated DegU.
messages: Required to deliver LXG toxins to target cells.
messages: Secreted
messages: The WXG motif and the C-terminal tail are crucial for secretion.

224308:BSU 25800
gene id: 224308:BSU_25800
uniprot id: P45945
names: Similarity
messages: To M.tuberculosis Rv2641.

224308:BSU 02330
gene id: 224308:BSU_02330
uniprot id: A0A6M3Z7G6
uniprot id: O31457
names: Function
names: Similarity
messages: Belongs to the TrhO family.
messages: Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.

224308:BSU 33190
gene id: 224308:BSU_33190
uniprot id: A0A6M4JP38
uniprot id: O34782
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 26420
gene id: 224308:BSU_26420
uniprot id: A0A6M3ZEI0
uniprot id: P54444
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YrkQ/YrkP. Probably activates YrkP by phosphorylation.
messages: Membrane

224308:BSU 27930
gene id: 224308:BSU_27930
uniprot id: P06535
names: Developmental Stage
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Expressed during sporulation.
messages: Homodimer. Dimerization is essential for activity as both monomers contribute to the formation of the active site.
messages: Key element in the phosphorelay regulating sporulation initiation. Acts on spo0A. Mediates reversible phosphoryl transfer from spo0F to spo0A.
messages: Phosphorylated by spo0F.

224308:BSU 16240
gene id: 224308:BSU_16240
uniprot id: P23445
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ATPase alpha/beta chains family.
messages: Cytoplasm
messages: Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum.

224308:BSU 28270
gene id: 224308:BSU_28270
uniprot id: A0A6M3ZEV2
uniprot id: P05645
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.
messages: Binds 1 Mg(2+) or Mn(2+) ion per subunit.
messages: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 34550
gene id: 224308:BSU_34550
uniprot id: A0A6M3ZJJ7
uniprot id: O06995
names: Cofactor
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Autophosphorylated.
messages: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
messages: Binds 2 magnesium ions per subunit.
messages: Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), and forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose (By similarity).
messages: Cytoplasm
messages: Homodimer.
messages: The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C(6)OH or C(1)OH, respectively (By similarity).

224308:BSU 22100
gene id: 224308:BSU_22100
uniprot id: A0A6M4JID3
uniprot id: P50846
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the KHG/KDPG aldolase family.
messages: Cytoplasm
messages: Homotrimer.
messages: Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.

224308:BSU 25750
gene id: 224308:BSU_25750
uniprot id: P42983
names: Cofactor
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Degrades both double-stranded linear and covalently closed circular DNA. Likely to play a scavenging role in order to supply nutrients under starvation conditions.
messages: Mn(2+) ion stimulates activity.
messages: Secreted
messages: Sporulation-specific.
messages: To B.subtilis NucA/ComI.

224308:BSU 11730
gene id: 224308:BSU_11730
uniprot id: O31622
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
messages: Has an important morphogenetic role. Involved in the assembly of at least five coat proteins, including CotB, CotG, CotS, CotSA and CotW. Required for appearance of a morphologically normal outer coat. To a large degree, CotO and CotH act at a late stage of coat assembly from within the outer coat to direct maturation of this structure.
messages: Largely but not entirely controlled by CotE.
messages: Spore coat

224308:BSU 07280
gene id: 224308:BSU_07280
uniprot id: O06485
names: Similarity
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.

224308:BSU 07880
gene id: 224308:BSU_07880
uniprot id: A0A6M4JJJ7
uniprot id: O35016
names: Activity Regulation
names: Function
names: Induction
names: Similarity
messages: Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
messages: By ethanol stress. Expression is sigma B-dependent.
messages: Dephosphorylates the phosphotyrosine-containing proteins. Involved in ethanol stress resistance.
messages: Efficiently inhibited by Cu(2+) ion, Zn(2+) ion and N-ethylmaleimide, while the addition of Mg(2+), Ca(2+) or Fe(3+) ions has minimal effect. Inhibited in a competitive manner by vanadate.

224308:BSU 15810
gene id: 224308:BSU_15810
uniprot id: P37817
names: Biotechnology
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
names: Subunit
messages: Coordinates cortex and coat assembly during sporulation (PubMed:22463703, PubMed:8231808). Associates with the spore coat protein SpoIVA and with the outer forespore membrane, thereby serving as a membrane anchor that tethers SpoIVA and the entire spore coat to the forespore surface (PubMed:17427285). May also serve as a competitive inhibitor of FtsH activity during sporulation (PubMed:9287010).
messages: Expressed during sporulation and is regulated by the mother cell-specific transcription factors sigma E and SpoIIID (PubMed:8231808). There is a lag-phase of approximately 2 hours between the onset of transcription and translation (PubMed:18820968). The 5' untranslated region negatively influences its own translation (PubMed:18820968).
messages: Forespore outer membrane
messages: Interacts with SpoIVA (PubMed:17427285). May interact with the ATP-dependent protease FtsH (PubMed:9287010).
messages: Nanomolar concentrations inhibit biofilm formation in situ, in S.aureus and in P.aeruginosa, suggesting it might be useful controlling bacterial infections.
messages: Preferentially localizes to slightly convex membranes (PubMed:25825747, PubMed:29102609). This preferential adsorption is accurately modeled as a two-step 'dash-and-recruit' mechanism: first, an initial binding event occurs with a faster on rate, then cooperative recruitment of additional SpoVM molecules follows (PubMed:29102609).
messages: Residues in the N-terminal region are required for proper localization.

224308:BSU 32390
gene id: 224308:BSU_32390
uniprot id: O32135
names: Similarity
messages: Belongs to the UPF0759 family.

224308:BSU 04700
gene id: 224308:BSU_04700
uniprot id: P40399
names: Activity Regulation
names: Function
messages: Positive regulator of sigma-B activity. Dephosphorylates RsbV in response to environmental stress conveyed from the RsbXST module.
messages: Stimulated by a long-lived interaction with RsbT.

224308:BSU 22790
gene id: 224308:BSU_22790
uniprot id: A0A6M3ZDH9
uniprot id: P08821
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: (Microbial infection) Interacts with Bacillus phage SP01 Gp46; the interaction replaces dsDNA from the hbs-DNA complex.
messages: Belongs to the bacterial histone-like protein family.
messages: Constitutively transcribed with 2 transcripts of 0.54 and 0.38 kb.
messages: Essential, it cannot be deleted; upon depletion cell growth slows.
messages: Histone-like DNA-binding protein which introduces negative supercoils in relaxed plasmid DNA in the presence of topoisomerase I. There are at least 20,000 monomers/cell (PubMed:8439548). Capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Binds evenly across chromosome, does not display a preference for AT content (PubMed:21085634). Binds ss- and dsDNA in a sequence non-specific manner; 8 nucleotides are sufficient to bind protein (PubMed:3595600).
messages: Homodimer.
messages: nucleoid

224308:BSU 11340
gene id: 224308:BSU_11340
uniprot id: A0A6M3Z9T4
uniprot id: O34340
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.
messages: Inhibited by cerulenin.
messages: Involved in the type II fatty acid elongation cycle (PubMed:11325930). Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor (PubMed:11325930). Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition (By similarity).
messages: Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.

224308:BSU 33230
gene id: 224308:BSU_33230
uniprot id: O34843
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the sigma-70 factor family.
messages: In the mid-exponential phase of growth under acidic conditions. Positively autoregulated, in the presence of RsoA. Down-regulated by YvrL under nonstress conditions.
messages: Interacts with RNA polymerase.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Together with its coactivator RsoA, positively regulates the expression of at least three operons, including oxdC-yvrL, sigO-rsoA and yvrJ. Required for the acid stress-dependent induction of the oxalate decarboxylase oxdC.

224308:BSU 18200
gene id: 224308:BSU_18200
uniprot id: O31824
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Degrades RNA oligonucleotides with a length of 5 nucleotides in a 3'- to 5'-direction. Less active on shorter RNA oligonucleotides and on those with a length of 24 nucleotides. Prefers RNA oligonucleotides containing adenines rather than cytosines.
messages: No visible phenotype. Cells show normal growth.

224308:BSU 00320
gene id: 224308:BSU_00320
uniprot id: P37541
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Essential for the phosphorelay during initiation of sporulation. May control the level of phosphorylated spo0A through spo0E activity during sporulation.

224308:BSU 00270
gene id: 224308:BSU_00270
uniprot id: A0A6M3Z6E5
uniprot id: P37536
names: Similarity
messages: Belongs to the Orn/Lys/Arg decarboxylase class-I family.

224308:BSU 31220
gene id: 224308:BSU_31220
uniprot id: P40747
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 28590
gene id: 224308:BSU_28590
uniprot id: P94544
names: Activity Regulation
names: Cofactor
names: Domain
names: Function
names: Similarity
names: Subunit
messages: In the C-terminal section; belongs to the PHP family.
messages: In the N-terminal section; belongs to the DNA polymerase type-X family.
messages: Monomer.
messages: Probably binds 2 divalent metal cations per N-terminal polymerase domain. Mn(2+) is more effective than Mg(2+) for DNA polymerase activity.
messages: Probably binds 3 Mn(2+) ions per C-terminal exonuclease domain. Mg(2+) cannot replace Mn(2+) for 3'-5' exonuclease activity.
messages: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a single-nucleotide gap, allowing further sealing of the resulting nick by a DNA ligase, points to a putative role in base excision repair (BER) during the B.subtilis life cycle. Moreover, also possesses a 3'-5' exonuclease activity able to edit unpaired 3'-termini in a gapped DNA substrate and likely involved in resecting unannealed 3'-termini during DNA repair. The same PolX molecule could perform the subsequent gap-filling step. Does not display 5'-deoxyribose 5'-phosphate (dRP) lyase activity, as predicted by the lack of the lysine and tyrosine residues responsible for the dRP lyase activity in some other PolX members.
messages: The 3'-5' exonuclease activity resides in the C-terminal PHP domain.
messages: The polymerization activity is inhibited in the presence of 2'-3'-dideoxynucleoside 5'-triphosphate (ddNTP).

224308:BSU 40660
gene id: 224308:BSU_40660
uniprot id: A0A6M4JR65
uniprot id: P37498
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 05360
gene id: 224308:BSU_05360
uniprot id: A0A6M3Z7S8
uniprot id: P96680
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 14270
gene id: 224308:BSU_14270
uniprot id: O31702
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the HesA/MoeB/ThiF family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein MoaD.
messages: Homodimer. Forms a stable heterotetrameric complex of 2 MoeB and 2 MoaD during adenylation of MoaD (By similarity).

224308:BSU 21920
gene id: 224308:BSU_21920
uniprot id: A0A6M4JIB0
uniprot id: P54166
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 28 family. UgtP subfamily.
messages: Cell membrane
messages: Cells displayed reduced and abnormal cell size/shape, no effect on flotillin cluster numbers or size.
messages: Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA).
messages: Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA). Also seems to be able to form beta-tetraglucosyl-DAG, although this glycolipid has not been found in B.subtilis membrane. UgtP can only use UDP-glucose as sugar donor.

224308:BSU 10920
gene id: 224308:BSU_10920
uniprot id: A0A6M3ZEK6
uniprot id: O06736
names: Similarity
messages: Belongs to the sulfate adenylyltransferase family.

224308:BSU 28640
gene id: 224308:BSU_28640
uniprot id: A0A6M3ZEU4
uniprot id: P17921
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.
messages: Binds 2 magnesium ions per tetramer.
messages: Cytoplasm
messages: Tetramer of two alpha and two beta subunits.

224308:BSU 12900
gene id: 224308:BSU_12900
uniprot id: A0A6M4JJ38
uniprot id: O34358
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S1C family.
messages: Cell membrane
messages: Degrades abnormal exported proteins and responsible for the propeptide processing of a natural pro-protein and for the maturation of a native protein. It also plays a prominent role in stress (heat shock, ethanol, puromycin and NaCl) resistance during active exponential growth (Probable).
messages: In contrast to other bacteria, in which inactivation of serine protease leads to thermosensitivity, inactivation of HtrA leads to an increased thermotolerance and an increased tolerance to hydrogen peroxide. Inactivation of both HtrA and HtrB leads to growth defects and to thermosensitivity.
messages: Inactivation results in compensating overexpression of YtvA, especially during stress conditions.
messages: Membrane
messages: Transcription is CssS dependent. Induced by heat shock during exponential growth and by heterologous amylases at the transition phase of the growth cycle. Negatively regulates its own expression during exponential growth and during heat shock.

224308:BSU 20960
gene id: 224308:BSU_20960
uniprot id: O31937
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32670
gene id: 224308:BSU_32670
uniprot id: A0A6M3ZJ83
uniprot id: O32162
names: Function
names: Similarity
names: Subunit
messages: Belongs to the UPF0051 (ycf24) family.
messages: Part of the SufBCD complex that contains SufB, SufC and SufD.
messages: The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation (By similarity).

224308:BSU 16860
gene id: 224308:BSU_16860
uniprot id: O31766
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M16 family.
messages: Binds 1 zinc ion per subunit.

224308:BSU 12600
gene id: 224308:BSU_12600
uniprot id: P54326
names: Similarity
messages: To B.subtilis YqbD.

224308:BSU 35760
gene id: 224308:BSU_35760
uniprot id: A0A6M3ZL66
uniprot id: P27621
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CDP-glycerol glycerophosphotransferase family.
messages: Catalyzes the addition of a single glycerol phosphate residue to the prenoldiphosphate-linked disaccharide, as a primer for polymerisation by TagF.
messages: Cell membrane
messages: Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.

224308:BSU 39880
gene id: 224308:BSU_39880
uniprot id: P46327
names: Cofactor
names: Similarity
messages: Belongs to the ROX family.
messages: Binds 1 Fe(2+) ion per subunit.

224308:BSU 24160
gene id: 224308:BSU_24160
uniprot id: A0A6M3ZDH1
uniprot id: P45856
names: Developmental Stage
names: Induction
names: Similarity
messages: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
messages: Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
messages: Subject to catabolite repression.

224308:BSU 12090
gene id: 224308:BSU_12090
uniprot id: P11863
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
messages: Inner spore coat protein which seems to play a role in germination.
messages: Present in an increased level in yabG mutant spores.
messages: Spore coat

224308:BSU 15880
gene id: 224308:BSU_15880
uniprot id: A0A6M3ZAP6
uniprot id: O34835
names: Function
names: Induction
names: Similarity
messages: Autoregulated.
messages: Belongs to the FapR family.
messages: Transcription factor involved in regulation of membrane lipid biosynthesis by repressing genes involved in fatty acid and phospholipid metabolism. Binds to the 5'-TTAGTANNNNNTANTAA-3' consensus sequence found in the promoter of fabHAF operon (containing fabHA and fabF genes), yhdO and fapR genes and prevents their expression. Its action is probably modulated by malonyl-CoA.
messages: Transcriptional factor involved in regulation of membrane lipid biosynthesis by repressing genes involved in fatty acid and phospholipid metabolism.

224308:BSU 34729
gene id: 224308:BSU_34729
uniprot id: C0H3R7
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 22190
gene id: 224308:BSU_22190
uniprot id: P50838
names: Similarity
messages: Belongs to the UPF0398 family.

224308:BSU 30010
gene id: 224308:BSU_30010
uniprot id: O34977
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 27010
gene id: 224308:BSU_27010
uniprot id: A0A6M4JM77
uniprot id: C0SPA5
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: By formaldehyde and methylgloxal, under the control of AdhR. Encoded in an operon with yraA.
messages: Cells lacking this gene show significantly reduced growth in the presence of formaldehyde but not methylglyoxal.
messages: Functions in the protection against aldehyde-stress.

224308:BSU 25540
gene id: 224308:BSU_25540
uniprot id: A0A6M3ZHC5
uniprot id: P22322
names: Developmental Stage
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination.
messages: Belongs to the peptidase A25 family.
messages: GPR transcription occurs during sporulation in forespore first by sigma-F and then by sigma-G.
messages: Homotetramer.
messages: Initiates the degradation of small, acid-soluble proteins during spore germination.
messages: Initiates the rapid degradation of small, acid-soluble proteins during spore germination.

224308:BSU 16030
gene id: 224308:BSU_16030
uniprot id: A0A6M4JGA6
uniprot id: O31741
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RNA methyltransferase TrmD family.
messages: Cytoplasm
messages: Homodimer.
messages: Specifically methylates guanosine-37 in various tRNAs.

224308:BSU 31370
gene id: 224308:BSU_31370
uniprot id: O05239
names: Similarity
messages: Belongs to the iron-containing alcohol dehydrogenase family.

224308:BSU 23460
gene id: 224308:BSU_23460
uniprot id: A0A6M4JLH2
uniprot id: P10728
names: Developmental Stage
names: Function
names: Similarity
messages: Belongs to the anti-sigma-factor family.
messages: Binds to sigma F and blocks its ability to form an RNA polymerase holoenzyme (E-sigma F). Phosphorylates SpoIIAA on a serine residue. This phosphorylation may enable SpoIIAA to act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma F from inhibition.
messages: Interaction with SpoIIAB inhibits sigma F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore.

224308:BSU 21860
gene id: 224308:BSU_21860
uniprot id: P54170
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
messages: Belongs to the bacilliredoxin family.
messages: Expression is up-regulated by cumene hydroperoxide (CHP).
messages: Mild sensitivity to cumene hydroperoxide (CHP) as indicated by growth inhibition assay. No effect on growth after NaOCl treatment.
messages: N-terminal Cys of the CXC active site motif can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation protects Cys residues against overoxidation by acting as a redox switch in response to oxidative stress.
messages: S-bacillithiolation is the formation of mixed disulfide bonds between protein thiols and the general thiol reductant bacillithiol (BSH) under oxidative stress. BSH is an equivalent of glutathione (GSH) in Firmicutes. This protein is a dithiol bacilliredoxin, which debacillithiolates (removes BSH) the S-bacillithiolated OhrR (OhrR-SSB) in vitro and in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Involved in maintaining redox homeostasis in response to disulfide stress conditions (PubMed:24313874). Has a redox potential of -130 mV. Displays weak protein disulfide isomerase activity in vitro (PubMed:19653655).

224308:BSU 27870
gene id: 224308:BSU_27870
uniprot id: P38032
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.
messages: Binds 1 FAD per subunit.
messages: Catalyzes the oxidation of L-aspartate to iminoaspartate, the first step in the de novo biosynthesis of NAD(+) (PubMed:18959769). Can use either oxygen or fumarate as electron acceptors, which allows the enzyme to be functional under aerobic and anaerobic conditions (PubMed:18959769).
messages: Cytoplasm
messages: Expression is repressed in the presence of nicotinic acid.
messages: Monomer. Homodimer.
messages: Mutant requires exogenous nicotinic acid for growth.

224308:BSU 39270
gene id: 224308:BSU_39270
uniprot id: A0A6M4JQQ2
uniprot id: P40739
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in beta-glucoside transport (By similarity).

224308:BSU 08660
gene id: 224308:BSU_08660
uniprot id: A0A6M4JGM6
uniprot id: P07784
names: Function
names: Induction
names: Similarity
messages: Belongs to the gamma-type SASP family.
messages: Monocistronically transcribed in the late sporulation phase and cotranscribed with mutY and/or fabL during exponential growth. However, sspE is not translated during this period.
messages: SASP are proteins degraded in the first minutes of spore germination and provide amino acids for both new protein synthesis and metabolism. These proteins may be involved in dormant spore's high resistance to UV light.

224308:BSU 34340
gene id: 224308:BSU_34340
uniprot id: A0A6M3ZH71
uniprot id: P71053
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance.
messages: Repressed by SinR.

224308:BSU 35260
gene id: 224308:BSU_35260
uniprot id: A0A6M4JPQ4
uniprot id: O34814
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Cells lacking this gene and ftsX, the next gene in the operon, delay sporulation onset, as a result of which they form a medial rather than polar septum at the onset of sporulation. This is presumably due to slower phosphorylation and activation of the spo0A transcriptional regulator. However, at later time points these cells undergo polar division and eventually form smaller than wild-type mature spores.
messages: Homodimer. Forms a membrane-associated complex with FtsX.
messages: Interacts with FtsX.
messages: Part of the ABC transporter FtsEX involved in cellular division.
messages: Part of the ABC transporter FtsEX involved in sporulation. May act as an importer, possibly at the top of a hierarchical cascade leading to the correct temporal initiation of sporulation. Acts upstream of the histidine kinases KinA, KinB and KinC, the RapA phosphatase and the Spo0A sporulation protein.

224308:BSU 04490
gene id: 224308:BSU_04490
uniprot id: P96605
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 06620
gene id: 224308:BSU_06620
uniprot id: A0A6M3Z852
uniprot id: O31498
names: Function
names: Similarity
messages: Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
messages: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

224308:BSU 12030
gene id: 224308:BSU_12030
uniprot id: O31647
names: Disruption Phenotype
names: Induction
messages: Deletion of this gene has no effect on mannose utilization.
messages: Up-regulated by mannose. Is under the control of ManR. Is subject to carbon catabolite repression (CCR) by glucose. Forms part of an operon with manP and manA.

224308:BSU 15370
gene id: 224308:BSU_15370
uniprot id: A0A6M4JHX9
uniprot id: O31726
names: Function
names: Similarity
names: Subunit
messages: Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.
messages: Homodimer.
messages: Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity.

224308:BSU 25730
gene id: 224308:BSU_25730
uniprot id: A0A6M3ZEA8
uniprot id: P54448
names: Function
names: Similarity
messages: Belongs to the 6-phosphogluconate dehydrogenase family.
messages: May act as NAD-dependent 6-P-gluconate dehydrogenase.

224308:BSU 36090
gene id: 224308:BSU_36090
uniprot id: O05219
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 16540
gene id: 224308:BSU_16540
uniprot id: A0A6M3ZB76
uniprot id: O31752
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CDS family.
messages: Cell membrane
messages: Membrane

224308:BSU 28190
gene id: 224308:BSU_28190
uniprot id: A0A6M4JM66
uniprot id: P38424
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.
messages: Binds GTP and GDP.
messages: Essential for growth, it cannot be disrupted. In depletion experiments cells become over 3-fold longer, are abnormally curved and nucleoids condense.
messages: Estimated to be present at 1000 copies per cell.
messages: Necessary for normal cell division and for the maintenance of normal septation.

224308:BSU 30020
gene id: 224308:BSU_30020
uniprot id: O32067
names: Induction
messages: By disulfide stress.

224308:BSU 23880
gene id: 224308:BSU_23880
uniprot id: Q7WY64
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Sensor protein that up-regulates translation of the secondary membrane protein insertase (MisCB/YqjG) when activity of the primary membrane protein insertase (MisCA/SpoIIIJ) is limited. Acts as a ribosome-nascent chain complex. When the primary membrane protein insertase activity or level is reduced, the membrane insertion of MifM is impaired, which induces arrest of MifM translation and unfolding of the mRNA hairpin. Unfolding leads to translation of the downstream gene, which encodes the secondary membrane protein insertase MisCB/YqjG. Translation arrest of MifM is mediated by interaction of its C-terminal domain with the ribosomal polypeptide exit tunnel. Undergoes multisite stalling, which may allow a sufficient duration of ribosomal stalling and consequently sufficient levels of MisCB/YqjG.

224308:BSU 18650
gene id: 224308:BSU_18650
uniprot id: A0A6M3ZEX1
uniprot id: O34819
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide lyase 1 family.
messages: Catalyzes the depolymerization of pectins of methyl esterification degree from 13 to 75%, with an endo mode of action. Cannot degrade polygalacturonate (By similarity).
messages: Secreted

224308:BSU 17850
gene id: 224308:BSU_17850
uniprot id: A0A6M3ZB38
uniprot id: P31080
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: 100-fold decrease in homologous recombination efficiency.
messages: Belongs to the peptidase S24 family.
messages: By mitomycin, requires an intact dinR gene (PubMed:1657879). An SOS-independent induction of dinR occurs during competence, does not require an intact dinR gene (PubMed:1657879).
messages: Expressed during the entire cell cycle with at least a threefold increase when cells develop competence.
messages: Following treatment with mitomycin C protein levels begin to decrease after a 5-min lag and do not return to their original levels for at least 90 min.
messages: Homodimer.
messages: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.
messages: Represses dinA, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'; some genes have a tandem consensus sequence and their binding is cooperative (PubMed:1657879, PubMed:8969214, PubMed:9555905). In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair; autocleavage is maximal at pH 11 in the absence of RecA and ssDNA (PubMed:8969214).

224308:BSU 21420
gene id: 224308:BSU_21420
uniprot id: O31983
names: Function
messages: May be involved in the secretion of the autolysin BlyA.

224308:BSU 13400
gene id: 224308:BSU_13400
uniprot id: O34398
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Binds 3 Mn(2+); 2 Mn(2+) for polymerase/primase activity and 1 for ligase activity.
messages: In the C-terminal section; belongs to the ATP-dependent DNA ligase family.
messages: In the N-terminal section; belongs to the LigD polymerase family.
messages: Interacts with Ku.
messages: LigD has variable architecture. In Bacillus lacks the 3'-phosphoesterase domain (PE) found in Mycobacteria and some Gammaproteobacteria.
messages: Stationary-phase cells lacking this gene are more sensitive to ionizing radiation (IR) than cells lacking Ku (YkoV); a double mutant is not more sensitive than the ku knockout. Spores lacking this gene are more sensitive to UV, IR, ultrahigh vacuum, and dry heat.
messages: The preference of the polymerase domain for rNTPs over dNTPs may be advantageous in dormant cells, where the dNTP pool may be limiting.
messages: Transcriptionally regulated by SpoVT and sigma-G factor.
messages: With Ku forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity (Probable). Probably involved in DNA repair during spore germination.

224308:BSU 10500
gene id: 224308:BSU_10500
uniprot id: O07561
names: Similarity
messages: Belongs to the PheA/TfdB FAD monooxygenase family.

224308:BSU 07955
gene id: 224308:BSU_07955
uniprot id: O34400
names: Caution
names: Cofactor
names: Similarity
messages: Belongs to the radical SAM superfamily.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Was originally (PubMed:8969503, PubMed:9384377) predicted to be the product of two different genes yfkA and yfkB.

224308:BSU 26480
gene id: 224308:BSU_26480
uniprot id: P54438
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 01050
gene id: 224308:BSU_01050
uniprot id: A0A6M3Z765
uniprot id: P02394
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL12 family.
messages: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.
messages: Homodimer. Part of the 50S ribosomal subunit; present in 4 copies per ribosome. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a pentameric L10(L12)2(L12)2 complex, where L10 forms an elongated spine to which 2 L12 dimers bind in a sequential fashion.
messages: Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.

224308:BSU 39960
gene id: 224308:BSU_39960
uniprot id: A0A6M4JQV5
uniprot id: P42108
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 07230
gene id: 224308:BSU_07230
uniprot id: O06489
names: Induction
messages: Repressed by YetL (PubMed:19329649). Induced by several flavonoids (PubMed:19329649).

224308:BSU 29230
gene id: 224308:BSU_29230
uniprot id: A0A6M3ZFD9
uniprot id: O34623
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DNA polymerase type-C family. DnaE subfamily.
messages: Cytoplasm
messages: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.
messages: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).
messages: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).
messages: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.

224308:BSU 36650
gene id: 224308:BSU_36650
uniprot id: A0A6M4JQ12
uniprot id: P71035
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the urease beta subunit family.
messages: Cytoplasm
messages: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.

224308:BSU 17840
gene id: 224308:BSU_17840
uniprot id: A0A6M4JGR5
uniprot id: O31817
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the fosfomycin resistance protein family. FosB subfamily.
messages: Cytoplasm
messages: Expression is sigma W-dependent.
messages: Homodimer.
messages: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor.

224308:BSU 08720
gene id: 224308:BSU_08720
uniprot id: Q796Y8
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.
messages: Monomer.
messages: The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.
messages: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

224308:BSU 14500
gene id: 224308:BSU_14500
uniprot id: A0A6M3ZAN3
uniprot id: P39759
names: Function
names: Similarity
messages: Belongs to the gamma-glutamylcyclotransferase family.
messages: Putative gamma-glutamylcyclotransferase.

224308:BSU 11390
gene id: 224308:BSU_11390
uniprot id: A0A6M4JHB5
uniprot id: P42062
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Membrane
messages: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transporting tripeptides. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 19690
gene id: 224308:BSU_19690
uniprot id: O34676
names: Biotechnology
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the radical SAM superfamily. KamA family.
messages: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the interconversion of L-alpha-lysine and L-beta-lysine.
messages: Deletion of the genomic region encompassing the entire yodT-yodS-yodR-yodQ-yodP-kamA gene cluster has no noticeable effect on growth either in rich or minimal medium, does not affect sporulation, and does not cause osmotic sensitivity or influence the compatible solute pool of this soil bacterium.
messages: Homotetramer.
messages: The use of KamA and YodP from B.subtilis for N6-acetyl-beta-lysine synthesis opens the bottleneck for the large-scale production of N6-acetyl-beta-lysine to investigate its properties as a compatible solute.

224308:BSU 11410
gene id: 224308:BSU_11410
uniprot id: A0A6M4JIM7
uniprot id: O31597
names: Similarity
messages: Belongs to the UPF0736 family.

224308:BSU 39050
gene id: 224308:BSU_39050
uniprot id: A0A6M3ZHW5
uniprot id: P42234
names: Function
names: Similarity
messages: Belongs to the catalase family. HPII subfamily.
messages: Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. Involved in sporulation.
messages: Serves to protect cells from the toxic effects of hydrogen peroxide.

224308:BSU 32570
gene id: 224308:BSU_32570
uniprot id: O32153
names: Function
names: Similarity
messages: Belongs to the carbohydrate kinase PfkB family.
messages: Catalyzes the phosphorylation of a range of fructosamines to fructosamine 6-phosphates.

224308:BSU 13610
gene id: 224308:BSU_13610
uniprot id: A0A6M4JFX3
uniprot id: O31668
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aldolase class II family. MtnB subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).
messages: Homotetramer.

224308:BSU 17130
gene id: 224308:BSU_17130
uniprot id: Q7PC63
names: Function
names: PTM
names: Subcellular Location Annotation
messages: 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by sfp.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 01480
gene id: 224308:BSU_01480
uniprot id: A0A6M4JF53
uniprot id: P70973
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the tRNA pseudouridine synthase TruA family.
messages: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 01800
gene id: 224308:BSU_01800
uniprot id: A0A6M3Z6W2
uniprot id: P37878
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the alkylbase DNA glycosidase AlkA family.
messages: Disruption of this gene sensitizes cells to the alkylating agent N-propyl-N'-nitro-N-nitrosoguanidine.
messages: Is involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Catalyzes the hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
messages: Overproduction of this gene renders cells highly resistant to the alkylating agent N-propyl-N'-nitro-N-nitrosoguanidine.
messages: Up-regulated by methylated AdaA in response to the exposure to alkylating agents.

224308:BSU 29170
gene id: 224308:BSU_29170
uniprot id: O32064
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0716 (FxsA) family.
messages: Cell membrane

224308:BSU 04790
gene id: 224308:BSU_04790
uniprot id: A0A6M3Z860
uniprot id: P96628
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SprT family.
messages: Binds 1 zinc ion.
messages: Cytoplasm

224308:BSU 08640
gene id: 224308:BSU_08640
uniprot id: O31585
names: Developmental Stage
names: Induction
messages: Expressed in mother cell during sporulation.
messages: Expression is sigma E-dependent.

224308:BSU 10520
gene id: 224308:BSU_10520
uniprot id: A0A6M4JEN0
uniprot id: O07563
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Can transport glucose, mannose, 2-deoxyglucose and methyl alpha-glucoside, but not galactose.
messages: Cell membrane
messages: Induced by glucose. Induction depends upon the integrity of the glucose-specific PTS system.
messages: Membrane

224308:BSU 27560
gene id: 224308:BSU_27560
uniprot id: A0A6M3ZEJ9
uniprot id: O32039
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 01430
gene id: 224308:BSU_01430
uniprot id: A0A6M3Z9W8
uniprot id: P20429
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RNA polymerase alpha chain family.
messages: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
messages: Homodimer. RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
messages: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.

224308:BSU 23620
gene id: 224308:BSU_23620
uniprot id: P54569
names: Similarity
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

224308:BSU 06460
gene id: 224308:BSU_06460
uniprot id: A0A6M3ZDD8
uniprot id: P12049
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PurS family.
messages: Cells lacking this gene show a purine auxotrophic phenotype and an accumulation of FGAR due to defective FGAM synthetase activity.
messages: Cytoplasm
messages: Homodimer or homotetramer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
messages: Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
messages: Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

224308:BSU 10040
gene id: 224308:BSU_10040
uniprot id: P55339
names: Function
names: Similarity
messages: Belongs to the ABC transporter superfamily.
messages: Has a role in exoprotein production, sporulation and competence.

224308:BSU 13850
gene id: 224308:BSU_13850
uniprot id: A0A6M3ZAC4
uniprot id: O31688
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
messages: By hydrogen peroxide. Repressed by PerR.
messages: Cell membrane
messages: Couples the hydrolysis of ATP with the transport of zinc into the cell. Plays an important role in protecting cells against oxidative stress. ZosA-mediated zinc transport is required for post-transcriptional control of comK and competence development.
messages: Disruption results in low transformability, which can be rescued by the addition of excess zinc.

224308:BSU 30990
gene id: 224308:BSU_30990
uniprot id: O32074
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Thiamine transporter subfamily.
messages: Cell membrane
messages: Cells display deregulation of thiamine biosynthesis and impaired export of thiamine products from the cell.
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of a membrane-embedded substrate-binding protein (S component), two ATP-binding proteins (A components) and a transmembrane protein (T component).
messages: Probably a thiamine-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (By similarity).

224308:BSU 25740
gene id: 224308:BSU_25740
uniprot id: P54447
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 19190
gene id: 224308:BSU_19190
uniprot id: O34757
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase. Acts as a sensor of the membrane fluidity. Probably activates DesR by phosphorylation.

224308:BSU 37820
gene id: 224308:BSU_37820
uniprot id: A0A6M3ZHU1
uniprot id: P39631
names: Function
names: Similarity
messages: Belongs to the dTDP-4-dehydrorhamnose reductase family.
messages: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.

224308:BSU 02150
gene id: 224308:BSU_02150
uniprot id: O31442
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 06170
gene id: 224308:BSU_06170
uniprot id: O34768
names: Similarity
messages: Belongs to the carbohydrate kinase PfkB family.

224308:BSU 15510
gene id: 224308:BSU_15510
uniprot id: A0A6M4JLN8
uniprot id: P25993
names: Similarity
names: Subunit
messages: Belongs to the CarA family.
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

224308:BSU 13160
gene id: 224308:BSU_13160
uniprot id: A0A6M4JKZ9
uniprot id: P80242
names: Function
names: Induction
names: Similarity
messages: Belongs to the OsmC/Ohr family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. Expression is sigma B-dependent.
messages: Involved in organic hydroperoxide resistance.

224308:BSU 35840
gene id: 224308:BSU_35840
uniprot id: A0A6M3ZJT3
uniprot id: Q7WY78
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the LytR/CpsA/Psr (LCP) family.
messages: Cell membrane
messages: Interacts with MreB.
messages: May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).
messages: Single mutant has no effect on cell growth or morphology under normal growth conditions. Triple disruption of tagTUV genes is not viable.

224308:BSU 18330
gene id: 224308:BSU_18330
uniprot id: A0A6M3ZBA9
uniprot id: P39846
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 2 phosphopantetheines covalently.
messages: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Tyr and Thr as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Thr residue is further converted to the D-allo-isomer form. The activation sites for these amino acids consist of individual domains.

224308:BSU 16120
gene id: 224308:BSU_16120
uniprot id: A0A6M4JLU8
uniprot id: P39814
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the type IA topoisomerase family.
messages: Binds two Mg(2+) per subunit.
messages: Monomer.
messages: Monomer. Interacts with the RNA polymerase core (PubMed:21710567).
messages: Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

224308:BSU 23190
gene id: 224308:BSU_23190
uniprot id: A0A6M3ZDD9
uniprot id: P35150
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S11 family.
messages: Expressed at about stage III of sporulation. Specifically enriched in stage IV forespores but not in stage IV mother cells (PubMed:3933484, PubMed:3080407).
messages: Forespore outer membrane
messages: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors (PubMed:3933484). Required specifically for the synthesis of the spore form of peptidoglycan (cortex) (Probable).

224308:BSU 29920
gene id: 224308:BSU_29920
uniprot id: C0SPC1
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activated by D-ribose and 2-deoxy-D-ribose. Slightly activated by kanamycin and gentamicin.
messages: Belongs to the aminoglycoside phosphotransferase family.
messages: Cells lacking this gene are slightly thinner and longer, but no anucleate cells are present and they have a growth rate similar to wild-type cells. Significantly decreased DNA replication initiation rate (PubMed:34373624). Susceptible to a broad range of DNA damage including mitomycin C (MMC), methyl methanesulfonate (MMS), ciprofloxacin, phleomycin and UV irradiation. Significant induction of the DNA damage response (SOS). Not sensitive to kanamycin or gentamicin (PubMed:35576203).
messages: Cytoplasm
messages: Monomer in solution. Interacts with DnaA (via domains I (1-82) and III (111-326)). Interacts with DnaB (PubMed:35576203). Interacts with FtsZ (By similarity).
messages: Plays a role in cell cycle regulation and chromosome integrity. Activates DnaA-dependent chromosomal DNA replication initiation ensuring that the chromosome is replicated at the right time during the cell cycle (By similarity). May regulate replication initiation through phosphorylation of a possible second messenger or metabolite, and by interacting with replication initiation proteins. Has ATPase activity with D-ribose and 2-deoxy-D-ribose in vitro, but not with choline. Involved in DNA damage response (PubMed:35576203).

224308:BSU 12550
gene id: 224308:BSU_12550
uniprot id: P54344
names: Similarity
messages: To B.subtilis YqaO.

224308:BSU 35780
gene id: 224308:BSU_35780
uniprot id: P39848
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 73 family.
messages: Cell wall hydrolase not involved in cell autolysis, competence, sporulation or germination. It hydrolyzes the beta-1,4 glycan bond between the N-acetylglucosaminyl and the N-acetylmuramoyl residues in the glycan chain.
messages: Cells lacking this gene show no motility changes on swarm plates; however in combination with an amidase deletion (lytC, AC Q02114) greatly reduced motility is seen.
messages: Homodimer.
messages: In stationary phase (PubMed:11987133); under control of SigD (PubMed:7581999).
messages: Inhibited by diethyl pyrocarbonate, slightly by EDTA. Not inhibited by PMSF, diisopropyl fluorophosphate, 2-mercaptoethanol or N-ethylmaleimide.
messages: Secreted
messages: cell wall

224308:BSU 03220
gene id: 224308:BSU_03220
uniprot id: P94392
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
messages: Catalyzes the high-affinity uptake of extracellular proline. Important for the use of proline as a sole carbon and energy source or a sole nitrogen source.
messages: Cell membrane
messages: Deletion of the putBCP operon abolishes L-proline utilization.
messages: The expression of the putBCP operon is induced in a PutR-dependent fashion by very low concentrations of L-proline in the growth medium. CodY represses the operon by displacing PutR from DNA.

224308:BSU 34980
gene id: 224308:BSU_34980
uniprot id: O34382
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37560
gene id: 224308:BSU_37560
uniprot id: A0A6M4JQA6
uniprot id: P18256
names: Caution
names: Cofactor
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Normally not expressed. Its expression is induced when that of thrS is reduced.

224308:BSU 36760
gene id: 224308:BSU_36760
uniprot id: A0A6M4JQ10
uniprot id: P70965
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EPSP synthase family. MurA subfamily.
messages: Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.
messages: Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Essential for cell growth.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 37280
gene id: 224308:BSU_37280
uniprot id: A0A6M3ZH93
uniprot id: P42175
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
messages: Cell membrane
messages: Membrane
messages: The alpha chain is the actual site of nitrate reduction.

224308:BSU 40960
gene id: 224308:BSU_40960
uniprot id: A0A6M4JRC9
uniprot id: P26497
names: Function
names: Similarity
messages: Belongs to the ParB family.
messages: Required for the initiation of sporulation and for normal chromosome segregation. Antagonizes sporulation inhibition by Soj. It probably interacts with a specific DNA site and other proteins involved in partitioning and cell division, and antagonizes Soj in response to cell cycle events related to chromosome partitioning.

224308:BSU 25840
gene id: 224308:BSU_25840
uniprot id: O32025
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Phr family.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably only subject to processing events at the ends of the mature peptide.
messages: Cytoplasm
messages: Inactivation of the gene leads to a reduction in sporulation efficiency.
messages: Secreted
messages: Signaling molecule involved in the regulation of sporulation (PubMed:10629174). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (Probable). Inhibitor of the RapE phosphatase activity (PubMed:10629174). Does not inhibit the phosphatase activity of RapA and RapB (PubMed:10629174). Probably plays a dispensable role in the overall context of sporulation initiation (PubMed:10629174).
messages: Transcription occurs independently of the rapE promoter and is controlled by the Spo0A-AbrB pair of transcriptional regulators.

224308:BSU 38930
gene id: 224308:BSU_38930
uniprot id: P94353
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 05810
gene id: 224308:BSU_05810
uniprot id: O05505
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II GmuABC PTS system is involved in the transport of oligo-glucomannans such as cellobiose or mannobiose.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 25070
gene id: 224308:BSU_25070
uniprot id: A0A6M3ZE63
uniprot id: P54482
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the IspG family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.

224308:BSU 14010
gene id: 224308:BSU_14010
uniprot id: P37599
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: PTM
messages: Cells lacking CheV are somewhat reduced in chemotactic proficiency, but a double mutant lacking both CheV and CheW has a strong tumble bias, does not respond to addition of attractant, shows essentially no accumulation in capillary assays, and has greatly reduced methyl turnover on the methyl-accepting chemotaxis proteins (MCPs).
messages: Consists of two domains: an N-terminal CheW-like coupling domain and a C-terminal two-component receiver domain.
messages: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. Chemotaxis involves both a phosphorylation-dependent excitation and a methylation-dependent adaptation. CheV and CheW are involved in the coupling of the methyl-accepting chemoreceptors to the central two-component kinase CheA; they are both necessary for efficient chemotaxis. Moreover, CheA-dependent phosphorylation of CheV is required for adaptation to attractants during B.subtilis chemotaxis.
messages: Peak expression is seen in early stationary phase.
messages: Phosphorylated by CheA.

224308:BSU 35130
gene id: 224308:BSU_35130
uniprot id: O34322
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 19020
gene id: 224308:BSU_19020
uniprot id: O34363
names: Similarity
messages: Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

224308:BSU 01560
gene id: 224308:BSU_01560
uniprot id: A0A6M3ZBZ3
uniprot id: P16449
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in the activation of the KinB signaling pathway of sporulation.
messages: No effect on sporulation as single mutant, but when associated with deletion of kinA, sporulation efficiency is significantly decreased.

224308:BSU 24670
gene id: 224308:BSU_24670
uniprot id: P25959
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homodimer; disulfide-linked. A minor fraction of ComGG is found as a disulfide-bonded homodimer.
messages: Partial processing of ComGG in competent cells requires ComC.
messages: Required for transformation and DNA binding.
messages: Secreted

224308:BSU 12800
gene id: 224308:BSU_12800
uniprot id: Q99163
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SPP1 holin family.
messages: Involved in cell lysis upon induction of PbsX.
messages: Membrane

224308:BSU 22070
gene id: 224308:BSU_22070
uniprot id: A0A6M3ZCY7
uniprot id: P42085
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family. Xpt subfamily.
messages: Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so it can be reused for RNA or DNA synthesis.
messages: Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so that it can be reused for RNA or DNA synthesis.
messages: Cytoplasm
messages: Highly specific for xanthine, with a strong discrimination against hypoxanthine and guanine as substrates.
messages: Homodimer.
messages: Repressed during growth on purines as a nitrogen source.

224308:BSU 18440
gene id: 224308:BSU_18440
uniprot id: O34399
names: Induction
names: Similarity
names: Subunit
messages: Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.
messages: Belongs to the glutamate synthase family.
messages: The gltAB operon is positively regulated by GltC and negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 14420
gene id: 224308:BSU_14420
uniprot id: O31715
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25690
gene id: 224308:BSU_25690
uniprot id: Q7WY62
names: Function
names: Miscellaneous
names: Subunit
messages: Forms a stable heterotetramer with KinA (comprising two molecules of each protein), by binding to the KinA dimerization/phosphotransfer domain.
messages: Mediates a developmental checkpoint inhibiting initiation of sporulation (by preventing phosphorylation of spo0A) in response to defects in the replication initiation machinery. Inhibits autophosphorylation of the histidine protein kinase KinA, forming a molecular barricade that prevents productive interaction between the ATP-binding site in the catalytic domain and the phosphorylatable His in the phosphotransfer domain of KinA. Probably also inhibits the activity of KinB, but has relatively little effect on KinC. Has at least one target in vivo in addition to KinA as sda does not require KinA to inhibit sporulation.
messages: Seems to be the major isoform.

224308:BSU 28140
gene id: 224308:BSU_28140
uniprot id: A0A6M4JNM3
uniprot id: P21248
names: Function
names: Similarity
names: Subunit
messages: Belongs to the uroporphyrinogen-III synthase family.
messages: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.
messages: Monomer.

224308:BSU 26790
gene id: 224308:BSU_26790
uniprot id: P17585
names: Biotechnology
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
names: Subunit
messages: Although this enzyme can be purified from B.subtilis, the bacteria is sensitive to streptomycin in vivo.
messages: Could be used as a selectable marker in B.subtilis if strongly expressed, could be used as a selectable marker in E.coli (Ref.7). Streptidine restores the antibiotic effectiveness of streptomycin in bacterial strains in which they both are co-administered together with a simultaneous overexpression of this enzyme (PubMed:17609790).
messages: Cytoplasm
messages: Homodimer.
messages: Mediates bacterial resistance to streptomycin (PubMed:3137862, PubMed:17609790). Adenylates streptomycin on the O-6 residue (PubMed:3137862, PubMed:15984036, PubMed:17609790). Adenylates streptidine on the O-6 residue (PubMed:17609790). Does not act on spectinomycin, neomycin-B or kanamycin (Ref.5, PubMed:15984036). Specific for ATP and GTP nucleotides incorporating a purine ring. No reaction with CTP or UTP (PubMed:15984036).
messages: Very slight reduction in streptomycin MIC.

224308:BSU 33270
gene id: 224308:BSU_33270
uniprot id: A0A6M3ZJB7
uniprot id: O34979
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 14870
gene id: 224308:BSU_14870
uniprot id: A0A6M3ZDZ2
uniprot id: P12946
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the COX15/CtaA family. Type 1 subfamily.
messages: Catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A.
messages: Cell membrane
messages: Interacts with CtaB.
messages: Membrane
messages: Mutations alter both cytochrome aa3 formation and sporulation.
messages: The N-half (TM1-TM4) and C-half (TM5-TM8) domains are connected by an intracellular loop. Each domain is formed from four-helix bundles and they align in a pseudo twofold symmetry manner. The N-half domain is the substrate-heme binding domain and the C-half domain is the cofactor heme binding domain.

224308:BSU 17400
gene id: 224308:BSU_17400
uniprot id: P50619
names: Disruption Phenotype
names: Induction
messages: Essential, at least the last 3 genes of the locus cannot be deleted; could be due to polar effects on downstream ymaB.
messages: Part of the probable nrdI(ymaA)-nrdE-nrdF-ymaB operon. Expression is constitutive but low, dramatically induced by thymidine starvation which requires recA.

224308:BSU 35060
gene id: 224308:BSU_35060
uniprot id: A0A6M4JPK4
uniprot id: O34926
names: Function
names: Similarity
names: Subunit
messages: Belongs to the cytochrome P450 family.
messages: Homodimer.
messages: Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe(3+). Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL.

224308:BSU 05340
gene id: 224308:BSU_05340
uniprot id: A0A6M4JDP1
uniprot id: P96678
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ArsB family.
messages: Cell membrane
messages: Involved in arsenical resistance. Thought to form the channel of an arsenite pump.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane

224308:BSU 29110
gene id: 224308:BSU_29110
uniprot id: A0A6M3ZF31
uniprot id: P13792
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system PhoP/PhoR involved in the regulation of alkaline phosphatase genes phoA and phoB and of phosphodiesterase.
messages: Phosphorylated by PhoR.

224308:BSU 17560
gene id: 224308:BSU_17560
uniprot id: P94487
names: Similarity
messages: To L.lactis TrpF C-terminal region.

224308:BSU 36600
gene id: 224308:BSU_36600
uniprot id: P71039
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Autoregulated.
messages: Cytoplasm
messages: Global transcriptional regulator that activates transcription of bmr and blt by binding directly to their promoter. Stimulates also the expression of the mta gene itself, ydfK and ymfE.
messages: Homodimer.
messages: The central dimerization domain of the first subunit forms a two-helix antiparallel coiled coil with the dimerization domain of the second subunit.

224308:BSU 14740
gene id: 224308:BSU_14740
uniprot id: O07628
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Anti-sigma factor for YlaC.
messages: Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily.
messages: Binds 1 Zn(2+) ion per subunit.
messages: Cell membrane
messages: The interaction between YlaC and YlaD may be regulated by the redox state of YlaD.

224308:BSU 28030
gene id: 224308:BSU_28030
uniprot id: A0A6M4JNV2
uniprot id: Q01465
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis has three MreB paralogs: MreB, Mbl and MreBH. All paralogs have the ability to support rod-shaped cell growth normal conditions. The multiplicity of paralogs becomes important under stress conditions. They are probably used to allow cells to maintain proper growth and morphogenesis under changing conditions.
messages: Belongs to the FtsA/MreB family.
messages: Cytoplasm
messages: Disruption of the gene results in loss of cell width control (PubMed:11290328). Depletion leads to a strong defect in chromosome segregation (PubMed:14588250, PubMed:15745453). It also leads to the formation of enlarged cells, to the loss of mid cell localization of the replication machinery and to growth arrest (PubMed:15745453). Double dynA-mreB deletions have strong cell shape defects (PubMed:23249255).
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination (PubMed:11290328, PubMed:15745453, PubMed:17064365, PubMed:19117023, PubMed:19659933). Acts by regulating cell wall synthesis and cell elongation, and thus cell shape (PubMed:19659933). A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature (By similarity). Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreB polymerization (PubMed:21636745, PubMed:21636744). The active movement of the MreB cytoskeleton stimulates diffusion of membrane proteins (PubMed:24603761). Organizes peptidoglycan synthesis in the lateral cell wall (PubMed:19659933). Also required for proper chromosome segregation (PubMed:14588250, PubMed:15745453, PubMed:17064365). Membrane fluidity influences MreB movement; in rich medium MreB movement is significantly decreased in a floA-floT mutant (PubMed:32662773).
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.
messages: Forms polymers (PubMed:11290328, PubMed:15745453, PubMed:17064365, PubMed:19117023). Forms a complex with Mbl and MreBH (PubMed:17064365, PubMed:19659933).
messages: Forms polymers.
messages: Polymerizes in the presence of millimolar divalent cations in a protein concentration-dependent manner. Polymerization is favored by decreasing pH and inhibited by monovalent salts and low temperatures.

224308:BSU 01110
gene id: 224308:BSU_01110
uniprot id: A0A6M3Z6J8
uniprot id: P21469
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS7 family.
messages: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts proteins S9 and S11 (By similarity). Interacts with VmlR (PubMed:30126986).
messages: Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.

224308:BSU 26690
gene id: 224308:BSU_26690
uniprot id: A0A6M3ZE94
uniprot id: P94499
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the branched chain amino acid transporter family.
messages: Branched-chain amino acid transport system which is involved in the uptake of isoleucine and valine (PubMed:25645558). Probably does not transport leucine (PubMed:25645558). Together with BcaP and BraB, plays an important role in the activation of CodY, a branched-chain amino acid-responsive transcriptional regulator that controls the expression of several dozen transcription units in B.subtilis (PubMed:25645558).
messages: Cell membrane
messages: Component of the transport system for branched-chain amino acids.
messages: Membrane

224308:BSU 00830
gene id: 224308:BSU_00830
uniprot id: A0A6M4JE25
uniprot id: P37568
names: Activity Regulation
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the CtsR family.
messages: Controls the expression of the cellular protein quality control genes clpC, clpE and clpP, as well as mcsA and mcsB. Acts as a repressor of these class III stress genes by binding to a directly repeated heptanucleotide operator sequence (A/GGTCAAA NAN A/GGTCAAA). After heat shock, CtsR is degraded by the ClpCP and ClpEP proteolytic systems, ensuring the derepression of clpE, clpP and the clpC operon. CtsR negatively autoregulates its own synthesis.
messages: CtsR autoinduces its own synthesis by derepression of the clpC operon after heat shock.
messages: Homodimer.
messages: Is degraded by ClpXP at 37 degrees Celsius, which maintains a basal level of the regulator within the cell. Is phosphorylated on Arg residues by McsB.
messages: Repressor activity is controlled via phosphorylation on arginine residues. Unphosphorylated CtsR binds with high affinity to its DNA consensus site and inhibits transcription of downstream genes, whereas the McsB-phosphorylated CtsR repressor is not able to bind to DNA, thus allowing heat-shock gene expression (By similarity).

224308:BSU 05260
gene id: 224308:BSU_05260
uniprot id: P96671
names: Similarity
messages: Belongs to the RBBP9 family.

224308:BSU 21440
gene id: 224308:BSU_21440
uniprot id: A0A6M3ZD22
uniprot id: P68571
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DsbB family. BdbC subfamily.
messages: Cell membrane
messages: Important but not absolutely essential for the production of the lantibiotic sublancin 168, it may also be required for the stability of other secreted proteins. Not required for competence for DNA uptake.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Required for disulfide bond formation in some proteins.

224308:BSU 08930
gene id: 224308:BSU_08930
uniprot id: A0A6M3Z8T6
uniprot id: O31590
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.
messages: Could methylate the ribose at the nucleotide 34 wobble position in tRNA.
messages: Cytoplasm

224308:BSU 30790
gene id: 224308:BSU_30790
uniprot id: A0A6M3ZFK0
uniprot id: P23971
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily.
messages: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl-CoA (OSB-CoA).

224308:BSU 40220
gene id: 224308:BSU_40220
uniprot id: Q45600
names: Similarity
messages: Belongs to the metallophosphoesterase superfamily.

224308:BSU 20190
gene id: 224308:BSU_20190
uniprot id: A0A6M3ZCE3
uniprot id: O31888
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Membrane

224308:BSU 21910
gene id: 224308:BSU_21910
uniprot id: A0A6M3ZGI8
uniprot id: P54167
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MetA family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.

224308:BSU 06940
gene id: 224308:BSU_06940
uniprot id: O31515
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 40340
gene id: 224308:BSU_40340
uniprot id: A0A6M3ZLX5
uniprot id: P38021
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. OAT subfamily.
messages: Catalyzes the interconversion of ornithine to glutamate semialdehyde.
messages: Cells lacking this gene do not grow in minimal medium containing either arginine or ornithine as sole nitrogen source.
messages: Cytoplasm
messages: Part of the rocDEF operon. Expression is sigma L dependent, induced by arginine, ornithine, ctirulline or proline. Ammonium and glutamine strongly repress induction of the rocD by proline, and only glutamine represses weakly induction by arginine.

224308:BSU 32010
gene id: 224308:BSU_32010
uniprot id: O32102
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the esterase D family.
messages: Catalyzes the hydrolysis of the trilactone cycle of ferri-bacillibactin (ferri-BB) complex, leading to the formation of bacillibactin monomers and to cytosolic iron release, thus making iron available for metabolic use. Can also hydrolyze bacillibactin (BB), however the catalytic efficiency for ferri-BB hydrolysis is much higher than for BB.
messages: Cytoplasm
messages: Impaired growth during iron limitation conditions and a strong accumulation of intracellular ferri-bacillibactin.
messages: Repressed by fur in the presence of iron.

224308:BSU 34200
gene id: 224308:BSU_34200
uniprot id: A0A6M3ZJI0
uniprot id: P24219
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Association with RNAP core increases slightly during late sporulation but not tested stresses (at protein level).
messages: Belongs to the sigma-54 factor family.
messages: Interacts transiently with the RNAP core.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA polymerase) serves as the receptor for receipt of the melting signal from the remotely bound activator protein LevR for the expression of the levanase operon. Associates with the RNAP core only in stationary phase cells (PubMed:21710567).

224308:BSU 02400
gene id: 224308:BSU_02400
uniprot id: A0A6M4JF22
uniprot id: O31462
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 02230
gene id: 224308:BSU_02230
uniprot id: A0A6M4JFH4
uniprot id: P39771
names: Function
names: Similarity
names: Subunit
messages: Belongs to the PurK/PurT family.
messages: Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP.
messages: Homodimer.
messages: Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.

224308:BSU 33360
gene id: 224308:BSU_33360
uniprot id: A0A6M3ZG90
uniprot id: O32206
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Although its sequence similarity with the glycerol phosphate lipoteichoic acid synthase LtaS, it cannot restore staphylococcal growth and LTA synthesis after ltaS depletion.
messages: Belongs to the LTA synthase family.
messages: Cell membrane
messages: Membrane
messages: Proteolytically cleaved.
messages: Secreted

224308:BSU 35540
gene id: 224308:BSU_35540
uniprot id: O32267
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 25060
gene id: 224308:BSU_25060
uniprot id: P54483
names: Subcellular Location Annotation
messages: Membrane
messages: cell wall

224308:BSU 28120
gene id: 224308:BSU_28120
uniprot id: A0A6M3ZFB7
uniprot id: P30949
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 07640
gene id: 224308:BSU_07640
uniprot id: A0A6M3Z8W2
uniprot id: O35031
names: Similarity
messages: Belongs to the acylphosphatase family.

224308:BSU 32280
gene id: 224308:BSU_32280
uniprot id: O32124
names: Similarity
messages: To B.subtilis YpjQ.

224308:BSU 03780
gene id: 224308:BSU_03780
uniprot id: P94416
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis is a well-characterized soil and water saprophyte, but it is also found in enteric flora of many species, including humans (Probable). In this environment, CSF can be transported into human intestinal epithelia via OCTN2, a host cell membrane transporter, and can induce cytoprotective heat shock proteins contributing to intestinal homeostasis (PubMed:18005709).
messages: Belongs to the Phr family.
messages: By mid-exponential phase, CSF accumulates to concentrations that stimulate ComA-dependent gene expression (PubMed:10464187). Upon entry into stationary phase, CSF reaches high concentrations that stimulate sporulation and inhibit ComA-dependent gene expression (PubMed:10464187).
messages: CSF, PhrF and PhrK stimulate ComA-dependent gene expression to different levels and are all required for full expression of genes activated by ComA.
messages: Cytoplasm
messages: Host cell
messages: In addition, in non-domesticated swarming strains of B.subtilis, the residual propeptide exposed on the exterior of the cytoplasmic membrane may have an extracellular role in swarming (PubMed:19202088). This function is probably not dependent on CSF (PubMed:19202088).
messages: Interacts with RapC and inhibits its interaction with ComA.
messages: Null mutant produces no detectable CSF activity, but null mutation has modest effects on competence gene expression (PubMed:8769645). Deletion of the gene results in decreased expression of genes activated by ComA, and significantly changes the expression of 66 operons (PubMed:16816200). In non-domesticated strains, the mutant is blocked early in swarming, forming stunted dendrites, with abnormal dendrite initiation morphology (PubMed:19202088). Mutant exhibits a specific migration defect that cannot be trans-complemented by CSF in a mixed swarm (PubMed:19202088).
messages: Part of the rapC-phrC operon, which is controlled by the P1 promoter (PubMed:10464187). Transcription from the P1 promoter is activated by high cell density through the phosphorylated form of ComA (PubMed:10464187). PhrC is part of an autoregulatory loop, and it positively regulates its own expression (PubMed:10464187). In addition, transcription of phrC is also regulated by a P2 promoter, which directs transcription of phrC only and is controlled by the sigma-H factor (PubMed:10464187).
messages: Secreted
messages: Secreted with a propeptide domain, which is cleaved in the cell wall by the secreted serine proteases subtilisin, Epr and Vpr to produce a mature signaling peptide (PubMed:17666034, PubMed:18689487). Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably subject to only one processing event, at the N-terminal end of the mature peptide (Probable).
messages: Signaling molecule that serves as a cell density signal for both genetic competence development and sporulation (PubMed:8769645, PubMed:9200610). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (PubMed:8769645, PubMed:9200610). At low concentrations, CSF stimulates expression of the genes controlled by ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:8769645, PubMed:9200610, PubMed:10464187). It includes the srfA operon, which encodes a small protein, ComS, required for competence development, and the surfactin biosynthetic enzymes (PubMed:8769645, PubMed:9200610, PubMed:10464187). Acts by inhibiting RapC, which regulates the activity of ComA (PubMed:8769645, PubMed:12950917). At high concentrations, it inhibits expression of those same ComA-controlled genes, maybe by inhibiting activity of the kinase ComP (PubMed:8769645, PubMed:9200610, PubMed:10464187). In addition, high concentrations of CSF can stimulate sporulation under some conditions (PubMed:8769645, PubMed:9200610). Also inhibits RapB activity, with lower efficiency, but does not act on RapA (PubMed:9238025). Is probably involved in the quorum sensing control of sporulation (Probable). CSF is a species-specific signaling molecule that partially compensates for the lack of ComX-mediated communication between different strains of B.subtilis (PubMed:18375560).

224308:BSU 37260
gene id: 224308:BSU_37260
uniprot id: P42178
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NarJ/NarW family.
messages: Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase.
messages: Cytoplasm

224308:BSU 35080
gene id: 224308:BSU_35080
uniprot id: P40762
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Deletion of the gene induces changes of proteins involved in cellular processes depending on iron availability. In iron-rich medium, mutants overproduce a red pigment after entry into the stationary growth phase.
messages: Expression increases during entry into stationary phase.
messages: Homodimer.
messages: Represses the expression of the yvmC-cypX operon, which is involved in pulcherriminic acid biosynthesis. Also negatively regulates yvmA, yvnB and its own expression. Positively regulates yisI expression. Acts by binding specifically to a 14-bp palindromic motif, the YvmB box, which is present in the promoter region of the target genes.
messages: Up-regulated in response to iron starvation. Negatively autoregulated. Also repressed by CcpA in stationary growth phase.

224308:BSU 15230
gene id: 224308:BSU_15230
uniprot id: A0A6M3ZAI8
uniprot id: P18579
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MurB family.
messages: Cell wall formation.
messages: Cytoplasm

224308:BSU 14260
gene id: 224308:BSU_14260
uniprot id: A0A6M3ZA82
uniprot id: O31701
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MobA family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.
messages: Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.

224308:BSU 01190
gene id: 224308:BSU_01190
uniprot id: A0A6M3Z6U0
uniprot id: P42919
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL2 family.
messages: One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.
messages: Part of the 50S ribosomal subunit (PubMed:10781545, PubMed:30126986). Forms a bridge to the 30S subunit in the 70S ribosome (By similarity).
messages: Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.

224308:BSU 17080
gene id: 224308:BSU_17080
uniprot id: O34381
names: Function
messages: Transcriptional regulation of the polyketide synthase operon.

224308:BSU 30420
gene id: 224308:BSU_30420
uniprot id: A0A6M4JKN6
uniprot id: O34392
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Expressed early in the stationary phase.
messages: Negatively regulated by YtrA.
messages: Part of the ABC transporter complex YtrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.
messages: The complex is composed of 2 ATP-binding proteins (YtrB and YtrE), 2 transmembrane proteins (YtrC and YtrD) and a solute-binding protein (YtrF).

224308:BSU 01130
gene id: 224308:BSU_01130
uniprot id: A0A6M3Z6X2
uniprot id: P33166
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.
messages: Cytoplasm
messages: Monomer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Monomer.
messages: Phosphorylated on Thr-385 in vitro by PrkC in the presence of poly-L-lysine or myelin basic protein, dephosphorylated by PrpC.
messages: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

224308:BSU 32940
gene id: 224308:BSU_32940
uniprot id: O32188
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Induced by iron starvation, partially repressed by fur.
messages: Provides the ATPase subunit for at least 2 ABC transporter complexes; YfiYZ/YfhA/YusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen (Probable), and FeuABC/YusV involved in import of the catecholate siderophores bacillibactin and enterobactin (Probable). Probably responsible for energy coupling to the transport system (By similarity).
messages: Strains lacking this gene show a reduction in growth stimulation by the iron-hydroxamate siderophores schizokinen and arthrobactin compared to wild-type. They also show a reduction in growth stimulation by the catecholate siderophores enterobactin and bacillibactin.
messages: The iron-hydroxamate siderophore complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (YfiZ and YfhA) and a solute-binding protein (YfiY); the catechoplate siderophore complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (FeuB and FeuC) and a solute-binding protein (FeuA).

224308:BSU 15770
gene id: 224308:BSU_15770
uniprot id: O34507
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Autophosphorylation on threonine residue(s) and serine residue considerably increases the kinase activity of the protein. Dephosphorylated in vitro by PrpC.
messages: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
messages: Bryostatin activates PrkC activity and induces germination, whereas staurosporine inhibits PrkC and significantly reduced peptidoglycan-dependent germination. Kinase activity of isolated N-terminus stimulated by poly-L-lysine or myelin basic protein (PubMed:19246764).
messages: Homodimer.
messages: Protein kinase that is responsible for triggering spore germination in response to muropeptides, signaling bacteria to exit dormancy. PrkC is thus a germination receptor that binds peptidoglycan fragments containing m-Dpm (meso-diaminopimelate), which act as spore germinants. Autophosphorylates and phosphorylates EF-G (elongation factor G, fusA); the latter modification is likely necessary for germination in response to peptidoglycan (PubMed:12399479). Another group did not detect phosphorylation of EF-G (PubMed:19246764). PrkC is a substrate in vitro of the cotranscribed phosphatase PrpC, which suggests that they form a functional couple in vivo. Might also be involved in sporulation and biofilm formation. Does not seem to be involved in stress response.
messages: PubMed:18984160 shows that peptidoglycan fragments serve as a novel mechanism of interspecies bacterial signaling that likely indicates the presence of growing bacteria and thus serve as a signal for dormant cells that growth-promoting conditions exist.
messages: Spore membrane
messages: Spores lacking this gene fail to germinate in the presence of peptidoglycan fragments or purified GlcNAc-MurNAc tripeptides and tetrapeptides. They still respond to the nutrient germinant L-alanine and to the chemical germinant Ca(2+)-dipicolinic acid, indicating that the spores are still capable of germinating and that PrkC is not involved in nutrient or chemical germination.
messages: The cytoplasmic domain has Ser/Thr kinase activity (PubMed:12406230). The C-terminal extracellular domain containing the PASTA repeats binds peptidoglycan.

224308:BSU 35220
gene id: 224308:BSU_35220
uniprot id: O34375
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MinJ family.
messages: Cell membrane
messages: Interacts directly with DivIVA and MinD.
messages: Mutants show defects in homologous recombination, swarming motility and cell division.
messages: The main function of the Min system is to promote the disassembly of the cytokinetic ring after cell division, thereby ensuring that division occurs only once per cell cycle. MinJ acts as a bridge between DivIVA and MinD. May modulate activity and localization of MinD and MinC through direct interaction with MinD.

224308:BSU 04710
gene id: 224308:BSU_04710
uniprot id: A0A6M4JF75
uniprot id: P17903
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the anti-sigma-factor antagonist family.
messages: Monomer (Probable). In stressed cells, forms a complex with RsbW. The predominant form of this complex has a stoichiometry of 2:2 (one dimer of RsbW is bound by two monomers of RsbV). Binds to RsbW in the presence of low levels of ATP or under conditions of energy or environmental stress (through dephosphorylation by RsbP or RsbU).
messages: Phosphorylated by RsbW on a serine residue. Dephosphorylated by RsbP or RsbU.
messages: Positive regulator of sigma-B activity. Non-phosphorylated RsbV binds to RsbW, preventing its association with sigma-B. When phosphorylated, releases RsbW, which is then free to complex with and inactivate sigma-B.

224308:BSU 29390
gene id: 224308:BSU_29390
uniprot id: O34350
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the acetyltransferase family.
messages: Growth rate decreased by 4-fold when grown in presence of taurine as sulfur source.
messages: Induced by glutathione but not sulfate (at protein level). Induced by methionine and taurine. Positively regulated by YtlI under supply of glutathione as sulfur source.
messages: Involved in a cysteine salvage pathway from S-alkylcysteine. Catalyzes the first step in this pathway, i.e. the amine acetylation of an S-alkylcysteine with a preference for S-benzyl-L-cysteine over S-methyl-L-cysteine. This pathway is likely important in the catabolism of alkylated cysteine generated by proteolysis of alkylated glutathione formed in the detoxification of a wide range of electrophiles.

224308:BSU 39240
gene id: 224308:BSU_39240
uniprot id: A0A6M3ZJS0
uniprot id: Q07835
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 26940
gene id: 224308:BSU_26940
uniprot id: O07918
names: Similarity
messages: Belongs to the glyoxalase I family.

224308:BSU 35500
gene id: 224308:BSU_35500
uniprot id: A0A6M3ZJQ8
uniprot id: P13799
names: Activity Regulation
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Autophosphorylated. Phosphorylated in vitro at Ser-76 by the serine/threonine-protein kinase YbdM, which stimulates the phosphate transfer to DegU.
messages: Cytoplasm
messages: Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase.
messages: Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation and biofilm formation. Acts as both a protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to DegU, and a protein phosphatase that dephosphorylates phospho-DegU.
messages: Regulated via serine phosphorylation of its input domain. Phosphotransfer from DegS to DegU is stimulated by phosphorylation on Ser-76 and by DegQ.

224308:BSU 03130
gene id: 224308:BSU_03130
uniprot id: A0A6M4JD15
uniprot id: P08164
names: Developmental Stage
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the NAD synthetase family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
messages: Homodimer.
messages: Phosphorylated.
messages: Synthesis starts during germination and outgrowth, and is highest at the end of exponential growth. Present in dormant spores.

224308:BSU 04760
gene id: 224308:BSU_04760
uniprot id: A0A6M3Z7W6
uniprot id: P96624
names: Similarity
messages: Belongs to the UPF0310 family.

224308:BSU 14830
gene id: 224308:BSU_14830
uniprot id: A0A6M3ZAE8
uniprot id: O07637
names: Similarity
names: Subunit
messages: Belongs to the glutaminase family.
messages: Homotetramer.

224308:BSU 19390
gene id: 224308:BSU_19390
uniprot id: O31850
names: Similarity
messages: Belongs to the CbbQ/NirQ/NorQ/GpvN family.

224308:BSU 15010
gene id: 224308:BSU_15010
uniprot id: O34331
names: Function
names: Similarity
messages: Belongs to the methyltransferase superfamily. RsmD family.
messages: May catalyze the S-adenosyl-L-methionine-dependent methylation of a specific base in rRNA.

224308:BSU 02980
gene id: 224308:BSU_02980
uniprot id: A0A6M3Z771
uniprot id: P46920
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine. Probably responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (OpuAA), two transmembrane proteins (OpuAB) and a solute-binding protein (OpuAC).

224308:BSU 02890
gene id: 224308:BSU_02890
uniprot id: A0A6M3ZAA8
uniprot id: P81100
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CAPAB/TerDEXZ family.
messages: Cytoplasm
messages: In response to low temperature and salt stress.

224308:BSU 35770
gene id: 224308:BSU_35770
uniprot id: P27622
names: Function
messages: Unknown. Might be involved in poly(glycerol phosphate) teichoic acid biosynthesis.

224308:BSU 39840
gene id: 224308:BSU_39840
uniprot id: P46331
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 16500
gene id: 224308:BSU_16500
uniprot id: A0A6M3ZEE9
uniprot id: P80700
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome (By similarity).
messages: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
messages: Belongs to the EF-Ts family.
messages: Cytoplasm

224308:BSU 28520
gene id: 224308:BSU_28520
uniprot id: A0A6M3ZJU3
uniprot id: P94551
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ETF alpha-subunit/FixB family.
messages: Binds 1 FAD per dimer.
messages: Heterodimer of an alpha and a beta subunit.
messages: The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity).

224308:BSU 39560
gene id: 224308:BSU_39560
uniprot id: P54946
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25580
gene id: 224308:BSU_25580
uniprot id: A0A6M4JLK7
uniprot id: P32393
names: Function
names: Similarity
messages: Belongs to the cytidine and deoxycytidylate deaminase family.
messages: Dispensable for transformability.

224308:BSU 10970
gene id: 224308:BSU_10970
uniprot id: O06741
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the mandelate racemase/muconate lactonizing enzyme family.
messages: Divalent metal cations. Mg(2+).
messages: Homooctamer.

224308:BSU 32230
gene id: 224308:BSU_32230
uniprot id: A0A6M4JMR1
uniprot id: P39839
names: Similarity
messages: Belongs to the peptidase S9C family.

224308:BSU 16920
gene id: 224308:BSU_16920
uniprot id: A0A6M4JK69
uniprot id: P46322
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
messages: Cell membrane
messages: Membrane
messages: This protein catalyzes the committed step to the synthesis of the acidic phospholipids.

224308:BSU 17340
gene id: 224308:BSU_17340
uniprot id: A0A6M3ZB27
uniprot id: O31796
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Hfq family.
messages: Homohexamer.
messages: RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.

224308:BSU 07620
gene id: 224308:BSU_07620
uniprot id: O34409
names: Cofactor
names: Induction
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Expression is under strict control of the medium composition. Induced by citrate, probably via the two-component regulatory system CitT/CitS. Repressed by rapidly metabolized carbon sources like glucose, glycerol and inositol, via the carbon catabolite repression system. Is also repressed by succinate and glutamate, albeit to a lesser extent.

224308:BSU 08760
gene id: 224308:BSU_08760
uniprot id: P71088
names: Function
names: Induction
names: Similarity
messages: Belongs to the spo0M family.
messages: Controls the expression of spo0A and is required to pass the morphological stage 0 of sporulation.
messages: Expression controlled by a sigma-H-regulated promoter which needs the sigma-H factor for the binding of the RNA polymerase and subsequent transcription.

224308:BSU 27760
gene id: 224308:BSU_27760
uniprot id: A0A6M3ZEH3
uniprot id: O05390
names: Developmental Stage
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. CsbX subfamily.
messages: Cell membrane
messages: Expressed during the stationary phase of cell growth.
messages: Membrane

224308:BSU 00630
gene id: 224308:BSU_00630
uniprot id: P37560
names: Similarity
messages: Belongs to the peptidase U57 family.

224308:BSU 38910
gene id: 224308:BSU_38910
uniprot id: A0A6M3ZLP3
uniprot id: P55184
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Phosphorylated by YxjM.
messages: Probable member of the two-component regulatory system YxjM/YxjL.

224308:BSU 36520
gene id: 224308:BSU_36520
uniprot id: A0A6M3ZHC0
uniprot id: Q07428
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the P(II) protein family.
messages: Cell membrane
messages: Cells show no growth phenotype even at acidic pH.
messages: It is uncertain whether Met-1 or Met-5 is the initiator.
messages: Needs to interact with NrgA in order to localize correctly to the membrane.
messages: Required for full induction of the nrgAB operon under conditions of ammonium limitation.
messages: The nrgAB operon is activated by TnrA under nitrogen-limited conditions.

224308:BSU 16230
gene id: 224308:BSU_16230
uniprot id: P23449
names: Function
names: Similarity
messages: Belongs to the FliH family.
messages: Needed for flagellar regrowth and assembly.

224308:BSU 07940
gene id: 224308:BSU_07940
uniprot id: A0A6M4JG26
uniprot id: O35043
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MscS (TC 1.A.23) family.
messages: Cell membrane
messages: Cells lacking this gene grow normally in minimal medium or in high-osmolarity environments, and exhibit the same survival capacity as the wild-type strain upon a drastic osmotic downshift. They sporulate and germinate normally.
messages: Induced by high salt concentrations.
messages: May play a role in resistance to osmotic downshock.
messages: Membrane

224308:BSU 04180
gene id: 224308:BSU_04180
uniprot id: P96576
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 34710
gene id: 224308:BSU_34710
uniprot id: O06979
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YvcQ/YvcP. Probably activates YvcP by phosphorylation.

224308:BSU 11030
gene id: 224308:BSU_11030
uniprot id: A0A6M4JKC6
names: Similarity
messages: Belongs to the CvfB family.

224308:BSU 00420
gene id: 224308:BSU_00420
uniprot id: A0A6M3Z712
uniprot id: P37468
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.
messages: Cytoplasm
messages: No visible phenotype. Not essential.
messages: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.

224308:BSU 32170
gene id: 224308:BSU_32170
uniprot id: A0A6M3ZG94
uniprot id: O32114
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the diaminopimelate epimerase family.
messages: Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 31190
gene id: 224308:BSU_31190
uniprot id: O05263
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the rhamnose mutarotase family.
messages: Cytoplasm
messages: Homodimer.
messages: Involved in the anomeric conversion of L-rhamnose.

224308:BSU 26850
gene id: 224308:BSU_26850
uniprot id: O05408
names: Similarity
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

224308:BSU 14280
gene id: 224308:BSU_14280
uniprot id: A0A6M4JJJ5
uniprot id: O31703
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the MoeA family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.

224308:BSU 25380
gene id: 224308:BSU_25380
uniprot id: A0A6M4JL38
uniprot id: P54466
names: Caution
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the flotillin-like FloA family.
messages: Cell membrane
messages: Few foci are seen on rich media, when cells are grown in minimal medium more foci are seen (at protein level) (PubMed:22753055). Constitutively expressed in rich and sporulation/biofilm-inducing media, not controlled by spo0A (at protein level) (PubMed:25909364).
messages: Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts FMMs are highly dynamic and increase in number as cells age. Flotillins are thought to be important factors in membrane fluidity.
messages: Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and increase in number as cells age. FloA and FloT function is partially redundant; double deletions have marked synthetic phenotypes (PubMed:20713508, PubMed:22753055, PubMed:25909364, PubMed:27362352). Flotillins are thought to be important factors in membrane fluidity, especially during periods of rapid growth in rich media (Probable). Whether specific proteins are associated with FMMs is controversial; in one study FloT rafts have been shown to include proteins involved in adaptation to stationary phase, while FloA-FloT rafts include proteins involved in differentiation including sporulation, biofilm formation and DNA uptake competence (PubMed:25909364). Another (more finely resolved) study only showed association of NfeD2 with FloT rafts of all the proteins examined (PubMed:27362352). Involved in spatial organization of membranes, perhaps recruiting proteins to specific membrane regions (PubMed:23651456). Simultaneous overexpression of both FloA and FloT leads to defects in cell division and differentiation, in part caused by stabilization of FtsH and its subsequent increased ability to degrade proteins. Cells make more biofilm, are about half as long, have less EzrA and more frequent Z-rings (PubMed:24222488).
messages: Homooligomerizes (PubMed:25909364). Interacts with FloT (PubMed:23651456, PubMed:25909364, PubMed:26297017). Interacts with FtsH midcell (Probable). Interacts with PhoR, colocalizes with PhoR in FloA-only membrane rafts (PubMed:25909364).
messages: Homooligomerizes.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane raft
messages: No effect on KinC activity, a double floT-floA deletion decreases the number of proteins in the DRM, blocks the ability of KinC to stimulate biofilm formation (PubMed:20713508). Single floA deletion has no change in FloT localization. Double floA-floT mutants have marked defects in cell morphology, motility, and transformation efficiency (PubMed:22753055). Single floA deletion sporulates less well. Double floA-floT deletion makes no biofilm, has greatly reduced FtsH, sporulates less than either single mutant (PubMed:22882210). Single mutation has a decrease in membrane fluidity, 25% decrease in protein secretion, double floT-floA deletion a stronger decrease in membrane fluidity and 35% decrease in protein secretion (PubMed:23651456). Double floA-floT deletion has reduced oligomerization of KinC (PubMed:26297017). Double floA-floT deletion cells are somewhat elongated, the site of cell wall synthesis is affected, increasing at division septa. The speed of MreB movement around the cell is significantly decreased in rich medium (PubMed:32662773).
messages: The last 95 residues are required for correct localization (PubMed:22753055). The C-terminus determines the oligomerization state of the protein; there are many small foci for FloA. Swapping with the C-terminus of FloT leads to fewer large foci (PubMed:25909364). N-terminally tagged, purified protein lacking the first 10 residues oligomerizes, with 12mer to about 50mer complexes found (PubMed:27362352).

224308:BSU 35210
gene id: 224308:BSU_35210
uniprot id: A0A6M4JPP2
uniprot id: O34502
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 30610
gene id: 224308:BSU_30610
uniprot id: O34314
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 32750
gene id: 224308:BSU_32750
uniprot id: O32169
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family.
messages: Cell membrane
messages: Part of the ABC transporter complex MetNPQ involved in methionine import (PubMed:14990259). Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in methionine sulfoxide transport (PubMed:14990259).
messages: Repressed by methionine via the S-box system.
messages: The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetP) and a solute-binding protein (MetQ).

224308:BSU 13140
gene id: 224308:BSU_13140
uniprot id: A0A6M3ZF74
uniprot id: O34762
names: Function
names: Induction
names: Similarity
messages: Belongs to the OsmC/Ohr family.
messages: Involved in organic hydroperoxide resistance.
messages: Strongly and specifically induced by organic peroxides. Repressed by OhrR.

224308:BSU 12380
gene id: 224308:BSU_12380
uniprot id: A0A6M3Z9W6
uniprot id: O34354
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the mannitol dehydrogenase family. UxaB subfamily.
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 28440
gene id: 224308:BSU_28440
uniprot id: A0A6M4JNM9
uniprot id: P08065
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
messages: Cell membrane
messages: In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558. Interacts with FloT (PubMed:23651456).
messages: Membrane raft

224308:BSU 07580
gene id: 224308:BSU_07580
uniprot id: O34427
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex.

224308:BSU 36150
gene id: 224308:BSU_36150
uniprot id: P96726
names: Function
names: Similarity
messages: Belongs to the SsuE family.
messages: Putative NADPH-dependent oxidoreductase.

224308:BSU 11110
gene id: 224308:BSU_11110
uniprot id: P70945
names: Function
messages: May bind long-chain fatty acids, such as palmitate, and may play a role in lipid transport or fatty acid metabolism.

224308:BSU 27340
gene id: 224308:BSU_27340
uniprot id: O32034
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the peptidase U32 family.
messages: Deletion mutant lacking both trhP1 and trhP2 shows reduced 5-methoxyuridine (mo5U) formation in tRNAs.
messages: Involved in prephenate-dependent formation of 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs, the first step in 5-methoxyuridine (mo5U) biosynthesis.

224308:BSU 01670
gene id: 224308:BSU_01670
uniprot id: O05213
names: Function
names: Similarity
messages: Belongs to the peptidase S12 family.
messages: Involved in muropeptide recycling. Hydrolyzes the amide bond between N-acetylmuramic acid (MurNAc) and the L-alanine residue of the stem peptide. Cannot hydrolyze muropeptides containing N-acetylglucosamine (GlcNAc) at the non-reducing end.

224308:BSU 02550
gene id: 224308:BSU_02550
uniprot id: A0A6M4JCU5
uniprot id: P42244
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YcbM/YcbL.
messages: Phosphorylated by YcbM.

224308:BSU 11809
gene id: 224308:BSU_11809
uniprot id: A0A6M3Z9J2
uniprot id: C0H3Y8
names: Developmental Stage
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Expressed during sporulation, under the control of SigK and GerE. Transcription begins 4 hours (T4) after the onset of sporulation.
messages: Membrane

224308:BSU 19490
gene id: 224308:BSU_19490
uniprot id: Q7WY67
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: Incorporated into the coat in two steps. First, independently of CotE, assembles into foci associated with the forespore. Next, in a CotE-dependent manner, spreads around the developing spore to form a stable contiguous shell.
messages: Induced in late sporulation stage by sigma K. Repressed by GerE.
messages: Involved in spore germination; probably required at the earliest stage of germination.
messages: Spore coat

224308:BSU 01410
gene id: 224308:BSU_01410
uniprot id: A0A6M3Z6P6
uniprot id: P20282
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS13 family.
messages: Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome (By similarity).
messages: Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.

224308:BSU 14180
gene id: 224308:BSU_14180
uniprot id: A0A6M4JJH4
uniprot id: O34981
names: Function
names: Similarity
messages: Belongs to the transferase hexapeptide repeat family. DapH subfamily.
messages: Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.

224308:BSU 33070
gene id: 224308:BSU_33070
uniprot id: A0A6M4JP26
uniprot id: P07870
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKC lipoprotein family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Forms a complex at the inner spore membrane which acts as a receptor for L-alanine, thus is involved in the stimulation of germination in response to alanine. Can stimulate germination in the absence of GerD and GerK gene products (fructose and glucose receptors, respectively), but the response is improved in their presence.

224308:BSU 06040
gene id: 224308:BSU_06040
uniprot id: O34672
names: Disruption Phenotype
names: Induction
messages: Encoded in an operon with groES, groEL, ydiN, ydiO and ydiP. This operon is heat-inducible.
messages: No BsuMI restriction or methylation-related phenotype.

224308:BSU 10670
gene id: 224308:BSU_10670
uniprot id: O06716
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the GerPA/GerPF family.
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 26260
gene id: 224308:BSU_26260
uniprot id: P45910
names: Similarity
messages: To B.subtilis YqxC and T.hyodysenteriae hemolysin TlyA.

224308:BSU 25590
gene id: 224308:BSU_25590
uniprot id: P39694
names: Developmental Stage
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expressed in cells competent for DNA transformation; that is 5-20% of the population (PubMed:16009133).
messages: Expression activated by ComK (PubMed:11918817).
messages: Needed for both DNA binding and transport. It is absolutely required for the uptake of transforming DNA but not for binding. Its role in binding may be indirect.

224308:BSU 15600
gene id: 224308:BSU_15600
uniprot id: A0A6M3ZFX5
uniprot id: O34577
names: Function
names: Induction
names: Similarity
messages: Belongs to the APS kinase family.
messages: Catalyzes the synthesis of activated sulfate.
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 11820
gene id: 224308:BSU_11820
uniprot id: A0A6M3Z9J8
uniprot id: O31626
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the helicase family. UvrD subfamily.
messages: Cytoplasm
messages: May be involved in the generation of recombinogenic substrates for the subsequent action of RecA.

224308:BSU 13460
gene id: 224308:BSU_13460
uniprot id: A0A6M4JFI2
uniprot id: O31655
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Anti-sigma factor for SigI. Negatively regulates SigI activity through direct interaction.
messages: Cell membrane
messages: Cells lacking this gene exhibit normal cell morphology and growth rate at 37 degrees Celsius, however cell diameter is slightly decreased.
messages: Interacts (via RsgI N-terminal anti-sigma domain) with SigI.
messages: Membrane
messages: Part of the sigI-rsgI operon, which is transiently induced by heat stress. Expression is positively regulated by SigI via the sigma-I promoter (PubMed:17185538). In exponentially growing cells, expression is regulated by the WalRK two-component system, which represses the sigma-I promoter and activates the sigma-A promoter, leading to the formation of a basal level of RsgI (PubMed:23199363). WalRK can also positively and directly regulate transcription of the operon under heat stress through a binding site located upstream of the sigma-I promoter (PubMed:24125693). Repressed by glucose (PubMed:17185538).

224308:BSU 34520
gene id: 224308:BSU_34520
uniprot id: A0A6M4JPG6
uniprot id: O06997
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the oxygen-dependent FAD-linked oxidoreductase family.
messages: Spore coat

224308:BSU 13100
gene id: 224308:BSU_13100
uniprot id: A0A6M3Z9V7
uniprot id: P49857
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. YkkC/YkkD subfamily.
messages: Cell membrane
messages: Deletion of both gdnC and gdnD leads to a decrease in the concentration of guanidine required to inhibit bacterial growth.
messages: Membrane
messages: Probably involved in guanidinium transport (PubMed:27989440). In vitro, confers resistance to a broad range of toxic compounds such as cationic dyes, neutral and anionic antimicrobials (PubMed:10735877).
messages: The efflux pump is composed of GdnC and GdnD.
messages: Transcriptionally regulated by guanidine, via a guanidine-sensing riboswitch.

224308:BSU 16845
gene id: 224308:BSU_16845
uniprot id: O31764
names: Caution
names: Similarity
messages: Belongs to the peptidase M16 family.
messages: In contrast to other members of the family, it lacks one conserved zinc-binding site and the active site.

224308:BSU 15310
gene id: 224308:BSU_15310
uniprot id: A0A6M3ZM43
uniprot id: P13801
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase U4 family.
messages: Cell membrane
messages: Does not affect the localization of SigE.
messages: Probable aspartic protease that is responsible for the proteolytic cleavage of the RNA polymerase sigma E factor (SigE/spoIIGB) to yield the active peptide in the mother cell during sporulation. Responds to a signal from the forespore that is triggered by the extracellular signal protein SpoIIR.
messages: Self-associates. Interacts with SigE (Probable). Interacts with SpoIIR.
messages: Self-associates. Interacts with SigE. Interacts with SpoIIR.

224308:BSU 01980
gene id: 224308:BSU_01980
uniprot id: O31430
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Required for production of the bacteriocin SkfA.
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 18310
gene id: 224308:BSU_18310
uniprot id: A0A6M3ZBN5
uniprot id: P94459
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 3 phosphopantetheines covalently.
messages: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Pro, Gln and Tyr as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Tyr residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.

224308:BSU 29320
gene id: 224308:BSU_29320
uniprot id: O34639
names: Function
names: Similarity
messages: Belongs to the glutaredoxin family.
messages: Involved in a cysteine salvage pathway from S-alkylcysteine. Catalyzes the reduction of N-acetyl-S-hydroxy-L-cysteine (N-acetyl-L-cysteine sulfenic acid) to N-acetyl-L-cysteine. This pathway is likely important in the catabolism of alkylated cysteine generated by proteolysis of alkylated glutathione formed in the detoxification of a wide range of electrophiles.

224308:BSU 33500
gene id: 224308:BSU_33500
uniprot id: A0A6M3ZJQ3
uniprot id: O32220
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
messages: By Cu(2+).
messages: Cell membrane
messages: Involved in copper export.
messages: Monomer at sub-stoichiometric copper concentrations. Homodimer at higher copper concentrations. Forms a heterodimer (electrostatic interactions) with CopZ during the transfer of Cu(+).
messages: The copZA operon is activated by CueR and indirectly repressed by YfmP.

224308:BSU 11130
gene id: 224308:BSU_11130
uniprot id: P39804
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Directly regulates the major intracellular proteinase (ISP-1) activity in vivo. Inhibits ISP-1 in the early stages of sporulation. It may be then inactivated by a membrane-bound proteinase.

224308:BSU 27020
gene id: 224308:BSU_27020
uniprot id: A0A6M4JLW9
uniprot id: O06006
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the peptidase C56 family.
messages: Functions in the protection against aldehyde-stress, possibly by degrading damaged proteins.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: No aldehyde-stress related phenotype; when combined with a yfkM disruption shows significantly reduced growth in the presence of formaldehye and methylglyoxal.
messages: The adhR-yraA operon is induced by formaldehyde and methylgloxal, under the control of AdhR (PubMed:19170879). A second yraA-specific transcription unit is not induced by formaldehyde or methylglyoxal and is not controlled by AdhR (PubMed:19170879). Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 14440
gene id: 224308:BSU_14440
uniprot id: A0A6M3ZAI6
uniprot id: O31717
names: Function
names: Similarity
messages: Belongs to the ketopantoate reductase family.
messages: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.

224308:BSU 33440
gene id: 224308:BSU_33440
uniprot id: A0A6M3ZGM1
uniprot id: O32214
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).
messages: Binds 1 FAD per subunit.
messages: Binds 1 FMN per subunit.
messages: Cells lacking the cysIJ genes are unable to use sulfate, sulfite or butanesulfonate as sole sulfur source, grow poorly with sulfide, but can still grow with thiosulfate, cysteine or methionine.
messages: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component (Probable).
messages: Up-regulated by sulfate and the transcriptional regulator CysL.

224308:BSU 04830
gene id: 224308:BSU_04830
uniprot id: O31490
names: Function
names: Induction
messages: Constitutively expressed at very low levels. Induced in response to overproduction of RapI or treatment with DNA-damaging agent mitomycin C (MMC).
messages: Required for the excision of the integrative and conjugative element ICEBs1. Excision of ICEBs1 requires two sites, attL and attR, at the left and right ends of the integrated ICEBs1.

224308:BSU 37640
gene id: 224308:BSU_37640
uniprot id: A0A6M3ZHK8
uniprot id: P39648
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the octanoyltransferase LipL family.
messages: Catalyzes the amidotransfer (transamidation) of the octanoyl moiety from octanoyl-GcvH to the lipoyl domain of the E2 subunit of lipoate-dependent enzymes.
messages: Cells lacking this gene are unable to grow in minimal medium. Addition of lipoic acid only partially restores growth of the mutant strain but the double mutant strain lacking both lipL and lplJ fails to grow in minimal medium containing lipoic acid. Moreover, the lipL mutant cells grow as well as wild-type in minimal medium supplemented with acetate and BCFA precursors.
messages: The reaction proceeds via a thioester-linked acyl-enzyme intermediate.

224308:BSU 04040
gene id: 224308:BSU_04040
uniprot id: P42962
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.
messages: Catalyzes the dephosphorylation of the riboflavin precursor 5-amino-6-(5-phospho-D-ribitylamino)uracil and of flavin mononucleotide (FMN) in vitro (PubMed:26316208). To a lesser extent, may also catalyze the dephosphorylation of a broad range of substrates such as phosphorylated sugars and triphosphate nucleotides in vitro (PubMed:26316208, PubMed:25848029).
messages: No visible phenotype during normal growth or under oxidative stress caused by diamide.

224308:BSU 23870
gene id: 224308:BSU_23870
uniprot id: A0A6M3ZGZ9
uniprot id: P54545
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DNA polymerase type-Y family.
messages: Binds 2 magnesium ions per subunit.
messages: Cytoplasm
messages: Monomer.
messages: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).
messages: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.

224308:BSU 34360
gene id: 224308:BSU_34360
uniprot id: P71051
names: Similarity
messages: Belongs to the CpsD/CapB family.

224308:BSU 27530
gene id: 224308:BSU_27530
uniprot id: O34528
names: Similarity
messages: Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.

224308:BSU 09320
gene id: 224308:BSU_09320
uniprot id: O07527
names: Function
messages: Member of the two-component regulatory system YhcY/YhcZ. Probably activates YhcZ by phosphorylation.

224308:BSU 38810
gene id: 224308:BSU_38810
uniprot id: P94360
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Deletion of the gene abolishes induction of the melREDCA operon the presence of melibiose and raffinose (PubMed:31138628). It does not affect growth in the presence of glucose and arabinose, but it has a negative effect on the ability of the mutant to grow on the alpha-1,5-arabinose oligomers alpha-1,5-arabinobiose, alpha-1,5-arabinotriose and alpha-1,5-arabinotetraose (PubMed:20693325). Deletion mutant cannot utilize pectin and galactan, and shows a slower but steady growth rate in the presence of type I rhamnogalacturonan or polygalacturonan (PubMed:29240795).
messages: Expressed in the presence of either arabinose or arabinotriose. Repressed by glucose.
messages: Required to energize different ABC-type saccharide transporters (PubMed:20693325, PubMed:29240795). Part of the MdxEFG-MsmX ABC transporter complex involved in maltodextrin import, of the AraNPQ-MsmX complex involved in arabinooligosaccharides import, of the GanPQS-MsmX complex involved in galactooligosaccharides import, and of the MelEDC-MsmX complex involved in melibiose, raffinose and stachyose import (PubMed:16707683, PubMed:20693325, PubMed:27501980, PubMed:29240795, PubMed:31138628). Is probably also part of the ABC transporter complex YtcQP-YteP-MsmX involved in polygalacturonan and rhamnogalacturonan type I import during pectin degradation (PubMed:29240795). Responsible for energy coupling to the transport system (Probable).
messages: The complex involved in maltodextrin import is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MdxF and MdxG) and a solute-binding protein (MdxE) (PubMed:16707683). The complex involved in arabinooligosaccharides uptake is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (AraP and AraQ) and a solute-binding protein (AraN) (PubMed:20693325). The complex involved in galactooligosaccharides uptake is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (GanP and GanQ) and a solute-binding protein (GanS) (PubMed:27501980, PubMed:29240795). The complex involved in melibiose, raffinose and stachyose import is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MelC and MelD) and a solute-binding protein (MelE) (PubMed:31138628). The complex involved in polygalacturonan and rhamnogalacturonan type I uptake is probably composed of two ATP-binding proteins (MsmX), two transmembrane proteins (YtcP and YteP) and a solute-binding protein (YtcQ) (PubMed:29240795).

224308:BSU 04650
gene id: 224308:BSU_04650
uniprot id: P96621
names: Function
names: Similarity
names: Subunit
messages: Antitoxin component of a type II toxin-antitoxin (TA) system. Antitoxin that directly inhibits activity of EndoA in vitro. Upon expression in E.coli counteracts inhibitory effect of endoribonuclease EndoA. The EndoA-EndoAI complex does not seem to bind its own promoter (PubMed:24120662).
messages: Belongs to the MazE/EndoAI family.
messages: Homodimer, forms a heterohexamer composed of alternating toxin and antitoxin homodimers which inhibits the toxin's endoribonuclease activity. Antitoxin prevents RNA binding to the endoribonuclease (PubMed:24120662).

224308:BSU 04430
gene id: 224308:BSU_04430
uniprot id: A0A6M3Z7P4
uniprot id: P80878
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the manganese catalase family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Binds 2 manganese ions per subunit.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Catalyzes the decomposition of hydrogen peroxide into water and oxygen.

224308:BSU 18070
gene id: 224308:BSU_18070
uniprot id: A0A6M3ZB80
uniprot id: Q45064
names: Activity Regulation
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PlsY family.
messages: Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.
messages: Cell membrane
messages: Inhibited by acyl-CoA.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Probably interacts with PlsX.

224308:BSU 25920
gene id: 224308:BSU_25920
uniprot id: P24810
names: Similarity
messages: To B.subtilis XkdY/XepA.

224308:BSU 28930
gene id: 224308:BSU_28930
uniprot id: A0A6M3ZJW2
uniprot id: P94513
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system LytS/LytT that probably regulates genes involved in cell wall metabolism.
messages: Membrane

224308:BSU 09080
gene id: 224308:BSU_09080
uniprot id: P54592
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 34910
gene id: 224308:BSU_34910
uniprot id: A0A6M4JPH8
uniprot id: O34651
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the histidinol dehydrogenase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.

224308:BSU 08320
gene id: 224308:BSU_08320
uniprot id: A0A6M4JG38
uniprot id: P94441
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-2 integral membrane protein family.
messages: Cell membrane
messages: Induced in response to linearmycins and other polyenes via the two-component regulatory system LnrJ/LnrK.
messages: Membrane
messages: Required for resistance to linearmycins, a family of antibiotic-specialized metabolites produced by some streptomycetes (PubMed:26647299, PubMed:28461449). Part of the ABC transporter complex LnrLMN that probably facilitates linearmycin removal from the membrane. Responsible for the translocation of the substrate across the membrane (PubMed:28461449). Also mediates KinC-dependent biofilm morphology (PubMed:28461449).
messages: The complex is composed of two ATP-binding proteins (LnrL) and two transmembrane proteins (LnrM and LnrN).

224308:BSU 17300
gene id: 224308:BSU_17300
uniprot id: A0A6M4JJE3
uniprot id: P0CW80
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. EbrA/EbrB subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons (By similarity).
messages: The efflux pump is composed of EbrA and EbrB.

224308:BSU 11460
gene id: 224308:BSU_11460
uniprot id: A0A6M3Z9N1
uniprot id: P24136
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Part of the binding protein-dependent transport system for oligopeptides. Probably responsible for energy coupling to the transport system. Required for sporulation and competence.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 08580
gene id: 224308:BSU_08580
uniprot id: O31580
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02320
gene id: 224308:BSU_02320
uniprot id: O31456
names: Function
messages: Probable transcriptional regulator.

224308:BSU 01040
gene id: 224308:BSU_01040
uniprot id: A0A6M4JEQ8
uniprot id: P42923
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL10 family.
messages: Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors (such as IF-2, EF-Tu, EF-G and RF3).
messages: Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.
messages: In response to low temperature and salt stress.
messages: Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and 23S rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a pentameric L10(L12)2(L12)2 complex.
messages: Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.

224308:BSU 38370
gene id: 224308:BSU_38370
uniprot id: P39586
names: Similarity
messages: Belongs to the glyoxalase I family.

224308:BSU 31590
gene id: 224308:BSU_31590
uniprot id: O05257
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 35250
gene id: 224308:BSU_35250
uniprot id: A0A6M3ZH03
uniprot id: O34876
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-4 integral membrane protein family. FtsX subfamily.
messages: Cell membrane
messages: Cells lacking this gene and ftsE, the previous gene in the operon, delay sporulation onset, as a result of which they form a medial rather than polar septum at the onset of sporulation. This is presumably due to slower phosphorylation and activation of the spo0A transcriptional regulator. However, at later time points these cells undergo polar division and eventually form smaller than wild-type mature spores.
messages: Interacts with FtsE (Probable). Interacts with FloT (PubMed:23651456).
messages: Membrane
messages: Membrane raft
messages: Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation.
messages: Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation. May act as an importer, possibly at the top of a hierarchical cascade leading to the correct temporal initiation of sporulation. Acts upstream of the histidine kinases KinA, KinB and KinC, the RapA phosphatase and the Spo0A sporulation protein.

224308:BSU 05130
gene id: 224308:BSU_05130
uniprot id: P96659
names: Similarity
messages: Belongs to the CarD family.

224308:BSU 40320
gene id: 224308:BSU_40320
uniprot id: A0A6M4JR10
uniprot id: P39138
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the arginase family.
messages: Binds 2 manganese ions per subunit.
messages: Cells lacking this gene do not grow on arginine as a sole nitrogen source, but do grow on ornithine or ammonium chloride.
messages: Involved in the catabolism of arginine.
messages: Part of the rocDEF operon. Expression is sigma L dependent, induced by arginine, ornithine or proline.

224308:BSU 25430
gene id: 224308:BSU_25430
uniprot id: P54462
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methylthiotransferase family. MtaB subfamily.
messages: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
messages: Cytoplasm

224308:BSU 10290
gene id: 224308:BSU_10290
uniprot id: A0A6M3ZEE8
uniprot id: P40769
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M48 family.
messages: Belongs to the peptidase M48B family.
messages: Binds 1 zinc ion per subunit.

224308:BSU 10760
gene id: 224308:BSU_10760
uniprot id: A0A6M3Z977
uniprot id: O06725
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0344 family.
messages: Cell membrane

224308:BSU 06180
gene id: 224308:BSU_06180
uniprot id: A0A6M3ZDA9
uniprot id: P54617
names: Induction
names: Similarity
messages: Belongs to the PspA/IM30 family.
messages: By cold shock (at protein level) (PubMed:8755892). Expression is sigma W-dependent; induced by alkali stress and by infection with phage SPP1 (PubMed:11454200).

224308:BSU 22680
gene id: 224308:BSU_22680
uniprot id: A3F3D1
uniprot id: P03963
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the anthranilate synthase component I family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Feedback inhibited by tryptophan.
messages: Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).
messages: Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).
messages: Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.

224308:BSU 00100
gene id: 224308:BSU_00100
uniprot id: A0A6M4JBY5
uniprot id: P08750
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S11 family.
messages: Cell membrane
messages: Membrane raft
messages: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
messages: cell wall

224308:BSU 26180
gene id: 224308:BSU_26180
uniprot id: A0A6M4JML3
uniprot id: P45917
names: Similarity
messages: Belongs to the phage portal family. PBSX subfamily.

224308:BSU 01210
gene id: 224308:BSU_01210
uniprot id: A0A6M4JC36
uniprot id: P42060
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL22 family.
messages: Part of the 50S ribosomal subunit.
messages: The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.
messages: This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity).
messages: This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.

224308:BSU 39320
gene id: 224308:BSU_39320
uniprot id: P42294
names: Disruption Phenotype
messages: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon has no visible growth phenotype, however it is out-competed by wild-type cells.

224308:BSU 32660
gene id: 224308:BSU_32660
uniprot id: O32161
names: Similarity
messages: Belongs to the glyoxalase I family.

224308:BSU 17280
gene id: 224308:BSU_17280
uniprot id: A0A6M4JGL9
uniprot id: O31790
names: Similarity
messages: Belongs to the OsmC/Ohr family.

224308:BSU 40970
gene id: 224308:BSU_40970
uniprot id: P37522
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the ParA family.
messages: Inhibits the initiation of sporulation, Spo0J antagonizes this inhibition. Soj ultimately inhibits the activation (phosphorylation) of Spo0A.
messages: Not required for sporulation or for chromosome partioning.

224308:BSU 21840
gene id: 224308:BSU_21840
uniprot id: P54172
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 14140
gene id: 224308:BSU_14140
uniprot id: A0A6M3ZAG3
uniprot id: O34827
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 36740
gene id: 224308:BSU_36740
uniprot id: P70960
names: Similarity
messages: To B.subtilis YwmD.

224308:BSU 07060
gene id: 224308:BSU_07060
uniprot id: O31527
names: Caution
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the polysaccharide lyase 11 family.
messages: Binds 10 calcium ions. The calcium may have a structural role.
messages: Divalent metal cations. Has highest activity with Mn(2+), followed by Zn(2+) and Co(2+).
messages: Monomer.
messages: Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases disaccharide products (PubMed:17449691, PubMed:19193638). Degrades rhamnogalacturonan, polygalacturonic acid and pectic acid. Has very low activity on pectin (PubMed:17449691).
messages: Secreted
messages: This enzyme is expected to be secreted, but there is no predicted signal sequence.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 00020
gene id: 224308:BSU_00020
uniprot id: A0A6M4JEE3
uniprot id: P05649
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the beta sliding clamp family.
messages: Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.
messages: Cytoplasm
messages: Forms a ring-shaped head-to-tail homodimer (PubMed:25170813) around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.
messages: Forms a ring-shaped head-to-tail homodimer around DNA.

224308:BSU 22130
gene id: 224308:BSU_22130
uniprot id: A0A6M3ZD57
uniprot id: P50843
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the KduI family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the isomerization of 5-dehydro-4-deoxy-D-glucuronate to 3-deoxy-D-glycero-2,5-hexodiulosonate.
messages: Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.

224308:BSU 32640
gene id: 224308:BSU_32640
uniprot id: Q7WY59
names: Developmental Stage
names: Induction
messages: Expressed only in the mother cell compartment of sporulating cells.
messages: Expression is GerE and sigma K-dependent.

224308:BSU 00790
gene id: 224308:BSU_00790
uniprot id: P29252
names: Function
names: Similarity
messages: Belongs to the HPPK family.
messages: Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.

224308:BSU 02960
gene id: 224308:BSU_02960
uniprot id: A0A6M4JCK1
uniprot id: O34724
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 33710
gene id: 224308:BSU_33710
uniprot id: Q45462
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the OsmX family.
messages: Cell membrane
messages: Member of a high affinity multicomponent binding-protein-dependent transport system for choline.
messages: Repressed by GbsR.

224308:BSU 39670
gene id: 224308:BSU_39670
uniprot id: A0A6M4JQW6
uniprot id: P42420
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the class II fructose-bisphosphate aldolase family. IolJ subfamily.
messages: Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.
messages: Produces dihydroxyacetone phosphate (DHAP or glycerone phosphate) and malonic semialdehyde (MSA or 3-oxopropanoate) from 6-phospho-5-dehydro-2-deoxy-D-gluconate (DKGP).

224308:BSU 09340
gene id: 224308:BSU_09340
uniprot id: O07529
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the azoreductase type 2 family.
messages: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH, but not NADH, as an electron donor for its activity.
messages: Homotetramer.
messages: Part of the yhcYZ-yhdA operon that is induced by the two-component regulatory system LiaS/LiaR in response to cell envelope stress.

224308:BSU 14890
gene id: 224308:BSU_14890
uniprot id: A0A6M3ZFQ3
uniprot id: P24011
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome c oxidase subunit 2 family.
messages: Binds a copper A center.
messages: Cell membrane
messages: Membrane
messages: Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).

224308:BSU 06220
gene id: 224308:BSU_06220
uniprot id: O34733
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 17860
gene id: 224308:BSU_17860
uniprot id: A0A6M3ZB60
uniprot id: Q45056
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YneA family.
messages: Cytoplasm
messages: Inhibits cell division during the SOS response. Affects a later stage of the cell division protein assembly, after the assembly of the Z ring, by probably suppressing recruitment of FtsL and/or DivIC to the division machinery (By similarity).
messages: Inhibits cell division during the SOS response. Affects a later stage of the cell division protein assembly, after the assembly of the Z ring, by probably suppressing recruitment of FtsL and/or DivIC to the division machinery.
messages: Repressed by LexA.

224308:BSU 10380
gene id: 224308:BSU_10380
uniprot id: O07621
names: Function
names: Similarity
names: Subunit
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Heme-containing signal transducer responsible for aerotaxis, the migratory response toward or away from oxygen.
messages: Homotetramer.

224308:BSU 35640
gene id: 224308:BSU_35640
uniprot id: Q02112
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Possible role in the secretion of LytB and LytC.
messages: Repressed by LytR.

224308:BSU 37980
gene id: 224308:BSU_37980
uniprot id: P39614
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.

224308:BSU 13200
gene id: 224308:BSU_13200
uniprot id: O34860
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Cells lacking this gene have an increased sigma-B activity in response to salt and ethanol stress and also increased sigma-B activity in response to the energy stress associated with entry into stationary phase. These results indicate that this protein is a negative regulator of sigma-B activity.
messages: Interacts with RsbRA and RsbS in the stressosome. The stressosome probably also contains RsbRC and RsbRD.
messages: Negative regulator of sigma-B activity. Non-phosphorylated RsbS binds to RsbT, preventing its association with RsbU. Requires any one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and the RsbR paralog(s) are phosphorylated, they release RsbT, which can then bind and activate RsbU.
messages: One of 4 functionally non-identical RsbR paralogs, it functions in the environmental signaling branch of the general stress response.
messages: Phosphorylated by RsbT.

224308:BSU 39070
gene id: 224308:BSU_39070
uniprot id: A0A6M3ZHM3
uniprot id: P04957
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 16 family.
messages: Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
messages: Secreted
messages: The sequence of strain 168 is shown.

224308:BSU 16620
gene id: 224308:BSU_16620
uniprot id: A0A6M3ZG82
uniprot id: P32729
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the eukaryotic ribosomal protein eL8 family.
messages: None; has no effect on viability, growth or sporulation at low, normal or elevated temperatures.
messages: RNA-binding protein that recognizes the K-turn motif present in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.

224308:BSU 23840
gene id: 224308:BSU_23840
uniprot id: A0A6M4JLJ9
uniprot id: P54548
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RNase Z family.
messages: Binds 2 Zn(2+) ions.
messages: Homodimer.
messages: Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.

224308:BSU 06090
gene id: 224308:BSU_06090
uniprot id: O35025
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CTCGAG-3'; the cleavage site is unknown.
messages: BsuMI restriction activity requires YdiR, YdiS and YdjA.
messages: Constitutively expressed during exponential growth. Encoded in an operon with ydiS and ydjA.
messages: Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences (PubMed:11751814). Triple deletion ydiO-ydiP-ydiS leads to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).
messages: Not expressed during sporulation.

224308:BSU 14210
gene id: 224308:BSU_14210
uniprot id: A0A6M3ZFK1
uniprot id: O34897
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MscS (TC 1.A.23) family.
messages: Cell membrane
messages: Cells lacking this gene grow normally in minimal medium or in high-osmolarity environments, and exhibit the same survival capacity as the wild-type strain upon a drastic osmotic downshift. They sporulate and germinate normally. Combination with an MscL mutation enhances the osmosensitivity of MscL mutants subsequent to a hypo-osmotic shock.
messages: Expression is sigma B-dependent. Induced by high salt concentrations.
messages: May play a role in resistance to osmotic downshock.
messages: Membrane

224308:BSU 12140
gene id: 224308:BSU_12140
uniprot id: O35027
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 13280
gene id: 224308:BSU_13280
uniprot id: O35012
names: Developmental Stage
names: Disruption Phenotype
names: Induction
names: Miscellaneous
messages: Cells lacking this gene show no noteworthy improvement of beta-toxoid secretion.
messages: Highly expressed in the late exponential phase.
messages: Overexpression of ykoJ is lethal to B.subtilis.
messages: Up-regulated under secretion stress conditions in a CssR/CssS-dependent manner.

224308:BSU 04320
gene id: 224308:BSU_04320
uniprot id: A0A6M3Z7K9
uniprot id: P96589
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: High-affinity potassium transporter (PubMed:28420751, PubMed:32005818). Functions as a K(+)/H(+) symporter (PubMed:32005818).
messages: Homodimer.
messages: Induced by low K(+) concentrations (PubMed:28420751). Expression is regulated by cyclic di-AMP (c-di-AMP), via the ydaO riboswitch (PubMed:20511502, PubMed:24141192, PubMed:28420751). High external concentrations of K(+) stimulate an increase in the production of c-di-AMP, which binds to the riboswitch and prevents kimA expression (PubMed:28420751).
messages: Membrane
messages: Potassium uptake increases at lower external pH and is abolished by the proton ionophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) (PubMed:32005818). Binds cyclic di-AMP (c-di-AMP), which inhibits the potassium transport activity (PubMed:31061098).

224308:BSU 19370
gene id: 224308:BSU_19370
uniprot id: A0A6M3ZBL3
uniprot id: P23129
names: Function
names: Similarity
names: Subunit
messages: Belongs to the alpha-ketoglutarate dehydrogenase family.
messages: E1 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the decarboxylation of 2-oxoglutarate, the first step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).
messages: Homodimer. Part of the 2-oxoglutarate dehydrogenase (OGDH) complex composed of E1 (2-oxoglutarate dehydrogenase), E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase); the complex contains multiple copies of the three enzymatic components (E1, E2 and E3).

224308:BSU 37780
gene id: 224308:BSU_37780
uniprot id: A0A6M3ZH77
uniprot id: P39634
names: Induction
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family. RocA subfamily.
messages: Expression is sigma L dependent and induced by arginine.

224308:BSU 23000
gene id: 224308:BSU_23000
uniprot id: P50731
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 02840
gene id: 224308:BSU_02840
uniprot id: O34364
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 13 family.
messages: By ethanol, heat and salt via sigma B-dependent promoter.
messages: Cytoplasm

224308:BSU 03010
gene id: 224308:BSU_03010
uniprot id: P54983
names: Similarity
messages: Belongs to the peptidase M20 family.

224308:BSU 10800
gene id: 224308:BSU_10800
uniprot id: A0A6M3Z9M1
uniprot id: Q7WY73
names: Similarity
messages: Belongs to the DinB family.

224308:BSU 38200
gene id: 224308:BSU_38200
uniprot id: A0A6M3ZHQ0
uniprot id: P39574
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GHMP kinase family. GalK subfamily.
messages: Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).
messages: Cytoplasm

224308:BSU 28670
gene id: 224308:BSU_28670
uniprot id: A0A6M3ZEZ9
uniprot id: P94536
names: Similarity
messages: Belongs to the CdaR family.

224308:BSU 01380
gene id: 224308:BSU_01380
uniprot id: A0A6M4JF38
uniprot id: P19994
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.
messages: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.
messages: Cytoplasm
messages: Expression increases gradually during the log phase of growth.
messages: Monomer.
messages: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.

224308:BSU 39970
gene id: 224308:BSU_39970
uniprot id: P42107
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in transport.

224308:BSU 33540
gene id: 224308:BSU_33540
uniprot id: A0A6M4JP80
uniprot id: O32224
names: Activity Regulation
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines (PubMed:17284825). Can reduce methyl red (PubMed:17284825).
messages: Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.
messages: Belongs to the azoreductase type 1 family.
messages: Binds 1 FMN per subunit.
messages: Deletion mutant is sensitive to 2-MHQ and catechol.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones (Probable). Contributes to resistance to 2-methylhydroquinone (2-MHQ) and catechol (PubMed:17725564, PubMed:18208493). Exhibits NADH-dependent 2,6-dichloroindophenol (DCIP) oxidoreductase activity (PubMed:17284825).
messages: Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.
messages: Repressed by MhqR. Induced by thiol specific stress conditions, such as exposure to 2-methylhydroquinone (2-MHQ), catechol or diamide. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.
messages: Strongly inhibited by Pb(2+) and weakly inhibited by Cu(2+), Hg(2+) and Fe(2+). Stable in presence of Ag(+).

224308:BSU 37430
gene id: 224308:BSU_37430
uniprot id: P71005
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in the production of the bacteriocin subtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 04500
gene id: 224308:BSU_04500
uniprot id: P96606
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 01120
gene id: 224308:BSU_01120
uniprot id: A0A6M3Z9U2
uniprot id: P80868
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.
messages: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).
messages: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
messages: Cytoplasm
messages: Phosphorylated on threonine residue(s). Phosphorylated by PrkC and dephosphorylated by PrpC, in vitro.

224308:BSU 03230
gene id: 224308:BSU_03230
uniprot id: A0A6M3ZAE1
uniprot id: P94393
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Activates transcription of the putBCP operon. Requires proline as a coactivator.
messages: Belongs to the CdaR family.
messages: Mutant loses the ability to utilize proline as either sole nitrogen or sole carbon source.
messages: PrcR expression is not subject to autoregulation and proline induction.

224308:BSU 13690
gene id: 224308:BSU_13690
uniprot id: A0A6M3ZA45
uniprot id: P28611
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MotA family.
messages: Cell membrane
messages: Each stator complex is composed of 4 MotA and 2 MotB subunits. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels (By similarity).
messages: Membrane
messages: MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel (By similarity).

224308:BSU 16490
gene id: 224308:BSU_16490
uniprot id: A0A6M4JGS3
uniprot id: P21464
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS2 family.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Interacts with BrxC (PubMed:33722570).

224308:BSU 10120
gene id: 224308:BSU_10120
uniprot id: A0A6M3Z936
uniprot id: P32395
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the uroporphyrinogen decarboxylase family.
messages: Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 02540
gene id: 224308:BSU_02540
uniprot id: A0A6M3Z7I7
uniprot id: P42243
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 15860
gene id: 224308:BSU_15860
uniprot id: A0A6M4JIQ9
uniprot id: O34607
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the iron-sulfur dependent L-serine dehydratase family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Heterodimer of an alpha chain and a beta chain.

224308:BSU 17600
gene id: 224308:BSU_17600
uniprot id: A0A6M3ZBR6
uniprot id: P0CI80
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the xylose isomerase family.
messages: Binds 2 magnesium ions per subunit.
messages: Cytoplasm
messages: Homotetramer.

224308:BSU 33770
gene id: 224308:BSU_33770
uniprot id: O34344
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: By Spo0A during nutrient starvation, through its direct negative control of AbrB (PubMed:12817086). Repressed by AbrB during regular growth when nutrients are plentiful, in association with the transcriptional repressor Abh (PubMed:17720793). Protein not detected during exponential growth, accumulates during stationary phase (at protein level) (PubMed:14568161).
messages: Produces a 42-residue extracellular sporulation delaying protein (SDP) that collapses the proton motive force (probably both the membrane potential and pH gradient) across the cell membrane, which leads to autolysis; may form a proton channel (PubMed:22469514). Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, inducing cannibalism to provide a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation (PubMed:12817086). Addition of SDP to liquid cultures halts growth, leads to increased cell permeability and eventually cell lysis in a significant subset of the population, although some cells survive and resume growth after a lag period (PubMed:20805502). Effects of SDP are irreversible within 10 minutes (PubMed:22469514). Addition of SDP to solid cultures induces killing, it is much more effective than SKF (AC O31422) (PubMed:20805502). Has antibiotic action against Gram-positive Firmicutes (L.acidophilus, M.megaterium, P.polymyxa, S.aureus, S.epidermidis) but not Actinobacteria M.luteus or Gram-negative P.aeruginosa or K.pneumoniae (PubMed:20805502, PubMed:22469514). SDP induces expression of the sdpR-sdpI operon (PubMed:12817086). Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, binds to SdpI triggering a signal transduction cascade that protects the cell against the toxic effects of its own SDP.
messages: Production of active SDP (able to induce SdpI and kill cells) is a multi-step process that requires signal peptide cleavage (probably by SipS or SipT) (PubMed:14568161) as well as SdpA and SdpB. The disulfide bond is not required for maximum toxicity (PubMed:23687264).
messages: Proprotein probably interacts with chaperone CsaA.
messages: Secreted
messages: When the sdpA-sdpB-sdpC operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086). In a single gene deletion no SDP is produced (PubMed:20805502, PubMed:23687264).

224308:BSU 04070
gene id: 224308:BSU_04070
uniprot id: A0A6M3ZKQ4
uniprot id: P42966
names: Similarity
messages: Belongs to the D-glutamate cyclase family.

224308:BSU 31960
gene id: 224308:BSU_31960
uniprot id: A0A6M3ZFN1
uniprot id: P45745
names: Caution
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Specifically adenylates L-threonine and, to a lesser extent, glycine and covalently loads both amino acids onto their corresponding peptidyl carrier domains.
messages: The phosphoserine observed at Ser-996 in PubMed:17218307 undoubtedly results from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.

224308:BSU 25790
gene id: 224308:BSU_25790
uniprot id: A0A6M4JLL9
uniprot id: P45946
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.
messages: Cell membrane
messages: Deletion of the arsB gene results in both an aresenite- and arsenate-sensitive phenotype in the absence of IPTG.
messages: Membrane
messages: Seems to confer resistance to arsenite by allowing cells to extrude this compound. Could be part of an arsenite extrusion pump.

224308:BSU 29550
gene id: 224308:BSU_29550
uniprot id: O34355
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily.

224308:BSU 28730
gene id: 224308:BSU_28730
uniprot id: A0A6M3ZEV3
uniprot id: P94530
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex AraNPQ involved in the uptake of arabinooligosaccharides. Transports alpha-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (PubMed:20693325). Responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (AraP and AraQ) and a solute-binding protein (AraN).
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 37100
gene id: 224308:BSU_37100
uniprot id: A0A6M3ZLD4
uniprot id: P19670
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EPSP synthase family. MurA subfamily.
messages: Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 17370
gene id: 224308:BSU_17370
uniprot id: A0A6M4JGL7
uniprot id: P50618
names: Function
names: Induction
names: Similarity
messages: Belongs to the NrdI family.
messages: Part of the probable nrdI(ymaA)-nrdE-nrdF-ymaB operon. Expression is constitutive but low, dramatically induced by thymidine starvation which requires recA.
messages: Probably involved in ribonucleotide reductase function.

224308:BSU 10260
gene id: 224308:BSU_10260
uniprot id: O07609
names: Induction
names: Similarity
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.
messages: By high salinity.

224308:BSU 19520
gene id: 224308:BSU_19520
uniprot id: O31862
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GntP permease family.
messages: Cell membrane

224308:BSU 09330
gene id: 224308:BSU_09330
uniprot id: A0A6M4JEQ1
uniprot id: O07528
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YhcY/YhcZ.
messages: Phosphorylated by YhcY.

224308:BSU 33222
gene id: 224308:BSU_33222
uniprot id: C0H3R4
names: Function
names: Induction
messages: Positively regulated by SigO.
messages: Together with RNA polymerase sigma factor SigO, positively regulates the expression of at least three operons, including oxdC-yvrL, sigO-rsoA and yvrJ. Required for the acid stress-dependent induction of the oxalate decarboxylase oxdC.

224308:BSU 24770
gene id: 224308:BSU_24770
uniprot id: A0A6M3ZDN8
uniprot id: P54503
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Activity is controlled at multiple levels (PubMed:22812682). Regulation includes a positive autoregulatory loop on mgsR transcription and a post-translational redox-sensitive activation step by an intramolecular disulfide bond formation in response to ethanol stress (PubMed:22812682). In addition, protein stability is strictly controlled by rapid proteolytic degradation by the ClpXP and ClpCP proteases (PubMed:22812682, PubMed:32477307). The McsB protein-arginine kinase might serve as a proteolytic adapter for the ClpX ATPase in the degradation mechanism of MgsR (PubMed:32477307).
messages: Belongs to the ArsC family.
messages: Cytoplasm
messages: Deletion of the gene results in differential expression of approximately 70 genes in response to ethanol stress compared with the wild-type strain.
messages: Expression is sigma B-dependent (PubMed:11544224, PubMed:18643936). Induced by ethanol, heat and salt stress (PubMed:11544224, PubMed:18643936). Positively autoregulated (PubMed:22812682).
messages: Regulates transcription of a subregulon within the general stress response (PubMed:18643936). Exerts positive and negative effects in response to ethanol stress (PubMed:18643936).

224308:BSU 24020
gene id: 224308:BSU_24020
uniprot id: P39075
names: Function
names: Subunit
messages: Activates transcription of the bmr gene in response to structurally dissimilar drugs. Binds rhodamine as an inducer.
messages: Binds DNA as a homodimer.

224308:BSU 28290
gene id: 224308:BSU_28290
uniprot id: A0A6M3ZI24
uniprot id: P37253
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ketol-acid reductoisomerase family.
messages: Binds 2 magnesium ions per subunit.
messages: Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 28840
gene id: 224308:BSU_28840
uniprot id: P94519
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 08710
gene id: 224308:BSU_08710
uniprot id: A0A6M4JLB2
uniprot id: P71084
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 31260
gene id: 224308:BSU_31260
uniprot id: A0A6M4JMS8
uniprot id: P39215
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpB is required for taxis towards asparagine, aspartate, glutamine, and histidine.
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft
messages: Only chemotaxis towards asparagine is completely deficient in the absence of McpB.
messages: Some glutamine residues are deamidated to glutamate by CheD and subsequently methylated.
messages: The demethylation is selective. Gln-371 is demethylated only upon asparagine addition whereas Glu-637 is demethylated only upon asparagine removal. Glu-630 appears indiscriminate and is demethylated upon both addition and removal of asparagine.

224308:BSU 29200
gene id: 224308:BSU_29200
uniprot id: A0A6M4JM40
uniprot id: O34847
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).
messages: Belongs to the AccA family.
messages: Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.
messages: Cytoplasm
messages: Inhibited by pyrrolidine dione antibiotics moiramide B (CPD1) and CPD2.

224308:BSU 00860
gene id: 224308:BSU_00860
uniprot id: A0A6M3Z752
uniprot id: P37571
names: Function
names: Similarity
messages: Belongs to the ClpA/ClpB family.
messages: Belongs to the ClpA/ClpB family. ClpC subfamily.
messages: Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with MecA to negatively regulate comK.

224308:BSU 12810
gene id: 224308:BSU_12810
uniprot id: P39800
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
messages: Secreted

224308:BSU 08029
gene id: 224308:BSU_08029
uniprot id: C0H3X6
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36510
gene id: 224308:BSU_36510
uniprot id: A0A6M4JPX4
uniprot id: Q07429
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ammonia transporter channel (TC 1.A.11.2) family.
messages: Cell membrane
messages: Cells grow poorly at pH 5.5 and not at all at pH 5.0 with ammonium as their sole nitrogen source (PubMed:14600241). They are unable to transport the ammonium analog methylammonium, and thus probably also ammonium (PubMed:14600241). In the absence of nitrogen source, cells lacking this gene do not show degradation of TnrA, which is entirely soluble (PubMed:17001076, PubMed:21435182).
messages: Functions as an ammonium and methylammonium transporter in the absence of glutamine (PubMed:14600241). Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source (PubMed:14600241). Required for binding of NrgB to the membrane (PubMed:14600241). Interaction between GlnK-AmtB complex and TnrA protects TnrA from proteolytic degradation (PubMed:17001076, PubMed:21435182).
messages: Interacts with NrgB for a correct localization of the latter. GlnK-AmtB complex interacts with TnrA.
messages: Membrane
messages: The nrgAB operon is activated by TnrA (PubMed:12823818). NrgB is required for full induction of the operon under conditions of ammonium limitation (PubMed:14600241, PubMed:10864496).

224308:BSU 25980
gene id: 224308:BSU_25980
uniprot id: P45935
names: Similarity
messages: Belongs to the Mu gp47/PBSX XkdT family.

224308:BSU 27550
gene id: 224308:BSU_27550
uniprot id: A0A6M3ZEF8
uniprot id: O32038
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
messages: Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 39230
gene id: 224308:BSU_39230
uniprot id: Q07833
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: A double wapA-wapI deletion strain is growth inhibited when cocultured with wild-type cells. When wapI is reintroduced it restores growth in a cognate toxin-dependent fashion (PubMed:23572593). Growth inhibition by wild-type cells is strongest on LB medium and less effective on media that promote biofilm formation (MSgg and 2xSGG) (PubMed:34280190).
messages: Belongs to the RHS/WapA nuclease family.
messages: Constitutively and highly expressed on solid and in liquid medium, with or without biofilm formation, by 12 hours of culture. Repressed by DegU.
messages: Detected in exponentially growing cells as many processing products, protein disappears upon entry into stationary phase with the concomitant appearance of smaller products. The large products persist in the absence of the extracellular serine protease Epr (PubMed:11987133).
messages: Has two ligand-binding domains; the N-terminus has three 101 AA repeats which are responsible for cell wall binding; the C-terminus consists of two blocks of residues with a conserved motif repeated 31 times.
messages: Identified in the extracellular proteome as many processing products ranging from over 85 kDa to about 30 kDa. One of these probably starts on Ser-1726. Two forms are known as CWBP62 and CWBP105 (PubMed:11987133).
messages: Secreted
messages: Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. A site-specific general tRNA nuclease, the C-terminus (residues 2201-2334) removes 2 or 4 nucleotides from the 3' end of at least 4 tRNAs (upon expression in E.coli), possibly endonucleolytically. The nuclease activity is neutralized by expression of the cognate immunity protein WapI from the same strain, but not its homolog from 2 other B.subtilis strains. The C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is necessary for growth inhibition (PubMed:23572593). Unlike the LXG toxin-immunity modules, WapAI mediates competition under shaking culture conditions (PubMed:34280190).
messages: cell wall

224308:BSU 37250
gene id: 224308:BSU_37250
uniprot id: A0A6M3ZH23
uniprot id: P42177
names: Cofactor
names: Function
names: Subcellular Location Annotation
messages: Binds 2 heme groups per subunit. Heme 1, called the proximal or heme Bp in, is located at the cytoplasmic interface, heme 2, called the distal or heme Bd, is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.
messages: Cell membrane
messages: Membrane
messages: The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

224308:BSU 37300
gene id: 224308:BSU_37300
uniprot id: P46909
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To H.influenzae HI_1626.

224308:BSU 15790
gene id: 224308:BSU_15790
uniprot id: A0A6M4JGL5
uniprot id: O34557
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ribulose-phosphate 3-epimerase family.
messages: Binds 1 divalent metal cation per subunit.
messages: Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.

224308:BSU 00030
gene id: 224308:BSU_00030
uniprot id: P05650
names: Disruption Phenotype
names: Function
names: Miscellaneous
messages: Disruption of the gene does not affect growth of the cell.
messages: Enhanced expression can restore both growth and sporulation of a temperature-sensitive mutation in rplB, designated rplB142.
messages: May assist in the assembly of the 50S subunit.

224308:BSU 08680
gene id: 224308:BSU_08680
uniprot id: A0A6M3Z989
uniprot id: Q796Z1
names: Similarity
messages: Belongs to the UPF0374 family.

224308:BSU 00750
gene id: 224308:BSU_00750
uniprot id: P28819
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Monomer. Heterodimer consisting of two non-identical subunits: a glutamine amidotransferase subunit (PabA) and a aminodeoxychorismate synthase subunit (PabB) (By similarity).
messages: Part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate (ADC), a precursor of p-aminobenzoate (PABA) and tetrahydrofolate. In the first step, a glutamine amidotransferase (PabA) generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by aminodeoxychorismate synthase (PabB) to produce ADC. PabA converts glutamine into glutamate only in the presence of stoichiometric amounts of PabB. Also involved in the biosynthesis of anthranilate (By similarity). Complements a glutamine amidotransferase-negative mutant (PubMed:2123867).
messages: Repressed by tryptophan.
messages: Requires tryptophan and has a partial requirement for p-aminobenzoic acid for growth.

224308:BSU 00410
gene id: 224308:BSU_00410
uniprot id: A0A6M4JDY1
uniprot id: P37547
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ribonuclease M5 family.
messages: Cytoplasm
messages: Divalent metal cations; Mg(2+) > Mn(2+) > Ca(2+).
messages: Not essential. Accumulation of precursor forms of 5S rRNA in total, ribosomes and polysome RNA preparations; smaller forms (126 and 135 nucleotides) are predominantly found in ribosomes and polysomes. Each of the 10 rrn operons gives rise to a different 5S rRNA precursor due to differences in their 3' ends.
messages: Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step.
messages: Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step. Releases 5'-phosphoryl and 3'-hydroxy termini.
messages: Requires ribosomal protein L18 (rplR) for catalysis; it can be replaced by 30% dimethylsulfoxide suggesting L18 functions as an rRNA folding chaperone.

224308:BSU 27330
gene id: 224308:BSU_27330
uniprot id: A0A6M4JLF4
uniprot id: O32033
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the uridine kinase family.
messages: Cytoplasm

224308:BSU 39010
gene id: 224308:BSU_39010
uniprot id: P42313
names: Similarity
messages: Belongs to the methyltransferase superfamily. RlmA family.

224308:BSU 30400
gene id: 224308:BSU_30400
uniprot id: A0A6M4JRH9
uniprot id: O34951
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system BceS/BceR involved in the regulation of bacitracin resistance. When activated by BceS, binds to the upstream region of the bceAB promoter and up-regulates the expression of these two genes.
messages: Phosphorylated by BceS.

224308:BSU 32410
gene id: 224308:BSU_32410
uniprot id: A0A6M3ZGK5
uniprot id: O32137
names: Cofactor
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the metallo-dependent hydrolases superfamily. Allantoinase family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Carboxylation allows a single lysine to coordinate two zinc ions.
messages: Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or allantoate are added during limiting-nitrogen conditions.
messages: Homotetramer.

224308:BSU 33200
gene id: 224308:BSU_33200
uniprot id: A0A6M3ZKS2
uniprot id: O34940
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SMP-30/CGR1 family.
messages: Binds 1 divalent metal cation per subunit.
messages: Cytoplasm

224308:BSU 32040
gene id: 224308:BSU_32040
uniprot id: A0A6M3ZG73
uniprot id: O32105
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 33560
gene id: 224308:BSU_33560
uniprot id: O32226
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39680
gene id: 224308:BSU_39680
uniprot id: P42419
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the IolI family.
messages: Binds 1 divalent metal cation per subunit. Can use Mn(2+), Fe(2+) or Co(2+).
messages: Involved in the reversible interconverion of 2-keto-myo-inositol (2KMI, inosose or 2,4,6/3,5-pentahydroxycyclohexanone) to 1-keto-D-chiro-inositol (1KDCI or 2,3,5/4,6-pentahydroxycyclohexanone).

224308:BSU 08590
gene id: 224308:BSU_08590
uniprot id: O31581
names: Similarity
messages: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

224308:BSU 19980
gene id: 224308:BSU_19980
uniprot id: O31872
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38640
gene id: 224308:BSU_38640
uniprot id: A0A6M3ZHI5
uniprot id: P94376
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 01510
gene id: 224308:BSU_01510
uniprot id: P70976
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 29560
gene id: 224308:BSU_29560
uniprot id: C0SPB0
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.

224308:BSU 02090
gene id: 224308:BSU_02090
uniprot id: A0A6M3ZC62
uniprot id: P54427
names: Similarity
messages: Belongs to the class-D beta-lactamase family.

224308:BSU 18160
gene id: 224308:BSU_18160
uniprot id: A0A6M4JGV3
uniprot id: Q45071
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 43 family.
messages: Cleaves arabinose units from O-2- or O-3-monosubstituted xylose residues, thereby assisting in arabinoxylan (AX) and short-chain arabinoxylo-oligosaccharide (AXOS) degradation. Is more active on wheat bran AXOS than on wheat water-extractable AX and rye water-extractable AX. Does not display endoxylanase, xylosidase or arabinanase activity.
messages: Secreted
messages: The CBM6 domain lost its carbohydrate binding capacity.

224308:BSU 05310
gene id: 224308:BSU_05310
uniprot id: A0A6M3Z7T6
uniprot id: P96675
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 39860
gene id: 224308:BSU_39860
uniprot id: A0A6M3ZHS5
uniprot id: P46329
names: Disruption Phenotype
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family.
messages: No effect on vanillin degradation.

224308:BSU 13520
gene id: 224308:BSU_13520
uniprot id: A0A6M3ZFB1
uniprot id: O31660
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the acyltransferase 3 family.
messages: Cell membrane

224308:BSU 07870
gene id: 224308:BSU_07870
uniprot id: A0A6M3Z8Y1
uniprot id: O35019
names: Similarity
messages: Belongs to the UPF0435 family.

224308:BSU 10540
gene id: 224308:BSU_10540
uniprot id: O07565
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.
messages: Homotetramer.
messages: Induced by neotrehalosadiamine.
messages: Involved in the biosynthesis of kanosamine (3-amino-3-deoxy-D-glucose), which is known to have antibiotic and antifungal properties, and to be a precursor of the antibiotic neotrehalosadiamine (3,3'-diamino-3,3'-dideoxy-alpha,beta-trehalose (NTD)). Catalyzes the dephosphorylation of kanosamine 6-phosphate to yield kanosamine. There is a trace amount of activity using glucosamine-6-phosphate.
messages: The production of neotrehalosadiamine is dormant in the wild-type strain. A mutation in the beta subunit of RNA polymerase activates the production of the neotrehalosadiamine (PubMed:14612444).

224308:BSU 05410
gene id: 224308:BSU_05410
uniprot id: P96685
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YdfH/YdfI. May activate YdfI by phosphorylation.

224308:BSU 30390
gene id: 224308:BSU_30390
uniprot id: O35044
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system BceS/BceR involved in the regulation of bacitracin resistance. Activates BceR in response to extracellular bacitracin.

224308:BSU 14220
gene id: 224308:BSU_14220
uniprot id: A0A6M4JFS6
uniprot id: O34564
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
messages: Cytoplasm
messages: Homodimer; disulfide-linked, upon oxidation.
messages: The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide.
messages: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.

224308:BSU 01780
gene id: 224308:BSU_01780
uniprot id: A0A6M4JEL1
uniprot id: P0CI73
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.
messages: Constitutively expressed, part of the cdaA-cdaR-glmM-glmS operon (PubMed:23192352).
messages: Cytoplasm
messages: Homodimer.

224308:BSU 22470
gene id: 224308:BSU_22470
uniprot id: P42981
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the PIGL family.
messages: Involved in bacillithiol (BSH) biosynthesis. Catalyzes the second step of the pathway, the deacetylation of N-acetylglucosaminylmalate (GlcNAc-Mal) to glucosamine malate (GlcN-Mal).
messages: Mutant shows a 2-fold reduction in bacillithiol levels. BshB1/bshB2 double mutant does not produce bacillithiol.

224308:BSU 27370
gene id: 224308:BSU_27370
uniprot id: A0A6M4JNK6
uniprot id: O34758
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transglycosylase MltG family.
messages: Cell membrane
messages: Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.

224308:BSU 36700
gene id: 224308:BSU_36700
uniprot id: A0A6M4JPZ4
uniprot id: P39757
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the radical SAM superfamily. MoaA family.
messages: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
messages: Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (By similarity). Required for both nitrate assimilation and respiration.
messages: Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
messages: Monomer and homodimer.

224308:BSU 41010
gene id: 224308:BSU_41010
uniprot id: A0A6M3ZKP5
uniprot id: P25812
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MnmG family.
messages: Cytoplasm
messages: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
messages: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.

224308:BSU 37650
gene id: 224308:BSU_37650
uniprot id: A0A6M3ZHM0
uniprot id: P39647
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.
messages: Cannot grow on sulfate, sulfite or butanesulfonate as sole sulfur source.
messages: Negatively autoregulated.
messages: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription.

224308:BSU 03970
gene id: 224308:BSU_03970
uniprot id: P94434
names: Disruption Phenotype
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells lacking this gene show no difference in growth under copper deprivation or copper excess conditions.
messages: Significantly induced under copper-limiting conditions.

224308:BSU 04080
gene id: 224308:BSU_04080
uniprot id: P60495
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the PxpB family.
messages: Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate (PubMed:28830929). In addition, is a potent inhibitor of the autophosphorylation reaction of kinase A (kinA) and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. Is an inhibitor of the catalytic domain of kinase A affecting the ATP/ADP reactions and not the phosphotransferase functions of this domain. The inhibition is non-competitive with respect to ATP (PubMed:9334321).
messages: Deletion of the gene enhances sporulation (PubMed:9334321). Deletion mutant grows less well than wild type on minimal medium with ammonium as nitrogen source and cannot grow on 5-oxoproline. Mutant lacks 5-oxoprolinase activity and accumulates 5-oxo-L-proline (PubMed:28830929).
messages: Forms a complex composed of PxpA, PxpB and PxpC (PubMed:28830929). Interacts with PxpC (KipA) (PubMed:9334321, PubMed:21050859, PubMed:28830929). Interaction with PxpC prevents the inhibitory action of PxpB (KipI) (PubMed:9334321, PubMed:21050859). Interacts with KinA. Two PxpB monomers bind via their C-domains at a conserved proline in the KinA dimerization and histidine-phosphotransfer (DHp) domain (PubMed:18823995).
messages: Induced by glucose when readily available sources of nitrogen, such as ammonia or glutamine, are scarce. Transcriptionally activated by TnrA and repressed by KipR.
messages: Was originally (PubMed:8574415, PubMed:8969502) thought to be a longer ORF that encodes what is now known to be pxpB (kipI) and pxpC (kipA).

224308:BSU 31610
gene id: 224308:BSU_31610
uniprot id: A0A6M3ZKI1
uniprot id: O05259
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit B family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 06630
gene id: 224308:BSU_06630
uniprot id: O34629
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane raft

224308:BSU 33140
gene id: 224308:BSU_33140
uniprot id: A0A6M3ZFY6
uniprot id: O32203
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane

224308:BSU 25280
gene id: 224308:BSU_25280
uniprot id: A0A6M4JJ76
uniprot id: P42095
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RecO family.
messages: Involved in DNA repair and RecF pathway recombination.
messages: Plays a role in DNA double-stranded break repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecR. Is recruited to repair centers, foci that are the site of double-strand break(s) after RecN and before RecF; may actively recruit RecF.
messages: Recruited to foci following DNA damage; probably interacts with RecF.
messages: nucleoid

224308:BSU 31390
gene id: 224308:BSU_31390
uniprot id: P80870
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Cytoplasm
messages: Found in association with the 30S subunit of the ribosome.

224308:BSU 13720
gene id: 224308:BSU_13720
uniprot id: A0A6M4JMG8
uniprot id: O31675
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the QueC family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).
messages: Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). Uses ammonia as nitrogen donor.
messages: Homodimer.

224308:BSU 24320
gene id: 224308:BSU_24320
uniprot id: A0A6M4JJ19
uniprot id: P54520
names: Function
names: Similarity
messages: Belongs to the NusB family.
messages: Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.

224308:BSU 26740
gene id: 224308:BSU_26740
uniprot id: A0A6M4JM60
uniprot id: O08469
names: Similarity
messages: Belongs to the cytochrome P450 family.

224308:BSU 00040
gene id: 224308:BSU_00040
uniprot id: A0A6M3Z6E7
uniprot id: P05651
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RecF family.
messages: Cytoplasm
messages: Recruited to foci following DNA damage; probably interacts with RecO.
messages: The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
messages: The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Is recruited to repair centers, foci that are the site of double-strand DNA break(s) after RecN and RecO; recruitment may depend on RecO.
messages: nucleoid

224308:BSU 08520
gene id: 224308:BSU_08520
uniprot id: A0A6M4JE48
uniprot id: O31575
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RecX family.
messages: Cytoplasm
messages: Modulates RecA activity.

224308:BSU 05000
gene id: 224308:BSU_05000
uniprot id: P96648
names: Domain
messages: The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

224308:BSU 25210
gene id: 224308:BSU_25210
uniprot id: A0A6M3ZDT3
uniprot id: P05096
names: Cofactor
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DnaG primase family.
messages: Binds 1 zinc ion per monomer.
messages: Binds two Mg(2+) per subunit.
messages: Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.
messages: Monomer. Interacts with DnaB.
messages: RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.

224308:BSU 18610
gene id: 224308:BSU_18610
uniprot id: A0A6M4JGZ8
uniprot id: C0SP89
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Transcriptionally regulated by sigma-D factor.

224308:BSU 39420
gene id: 224308:BSU_39420
uniprot id: A0A6M3ZLR8
uniprot id: P39121
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.
messages: Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.
messages: Cytoplasm
messages: Induced by deoxyadenosine and thymidine. Repressed by DeoR and glucose.

224308:BSU 28830
gene id: 224308:BSU_28830
uniprot id: P94520
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expressed under the control of the sigma-W factor.
messages: May mediate a negative feedback loop that down-regulates the expression of the sigma-W regulon following the activation of sigma-W in response to conditions of cell envelope stress. Might interact with and inhibit the activity of the protease PrsW, or could bind to the anti-sigma-W factor RsiW and thereby protect it from PrsW-mediated cleavage.
messages: Membrane

224308:BSU 11870
gene id: 224308:BSU_11870
uniprot id: A0A6M4JRI7
uniprot id: O31631
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the trans-sulfuration enzymes family.
messages: Catalyzes the formation of L-cystathionine from O-acetyl-L-homoserine and L-cysteine. Cannot use O-succinyl-L-homoserine as substrate. Also exhibits O-acetylhomoserine thiolyase activity, catalyzing the synthesis of L-homocysteine from O-acetyl-L-homoserine and sulfide.
messages: Cells lacking this gene are unable to grow in the presence of sulfate or cysteine as sole sulfur source, whereas their growth in the presence of homocysteine, cystathionine or methionine is similar to that of the wild-type strain.
messages: Cytoplasm
messages: Homotetramer.
messages: Up-regulated at the transcriptional level when sulfate, cysteine, cystathionine or homocysteine are the sulfur sources. Repressed by methionine.

224308:BSU 36340
gene id: 224308:BSU_36340
uniprot id: P94587
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the bacterial sortase family.
messages: Compared to other sortases, it is shorter and lacks the N-terminal membrane anchor. It may lack activity.
messages: Mutation does not affect extracellular levels of the sortase substrate YfkN.
messages: Preferentially expressed in the late stationary phase.
messages: Seems not to play a major role if any as a sortase.

224308:BSU 29970
gene id: 224308:BSU_29970
uniprot id: A0A6M3ZF59
uniprot id: O34476
names: Similarity
messages: Belongs to the cysteine synthase/cystathionine beta-synthase family.

224308:BSU 15060
gene id: 224308:BSU_15060
uniprot id: A0A6M4JHV3
uniprot id: O34513
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TmcAL family.
messages: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac-AMP) and then transfers the acetyl group to tRNA to form ac(4)C34.
messages: Cytoplasm
messages: Deletion mutant lacks the ac(4)C34 modification and displays a cold-sensitive phenotype.
messages: Homodimer.

224308:BSU 27150
gene id: 224308:BSU_27150
uniprot id: O05401
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30200
gene id: 224308:BSU_30200
uniprot id: A0A6M3ZFW1
uniprot id: P53557
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the radical SAM superfamily. Biotin synthase family.
messages: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.
messages: Homodimer.

224308:BSU 34670
gene id: 224308:BSU_34670
uniprot id: A0A6M3ZJK5
uniprot id: O06983
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the beta-class carbonic anhydrase family.
messages: Binds 1 zinc ion per subunit.
messages: Reversible hydration of carbon dioxide.

224308:BSU 06420
gene id: 224308:BSU_06420
uniprot id: A0A6M4JKS5
uniprot id: P12044
names: Function
names: Similarity
messages: Belongs to the AIR carboxylase family. Class I subfamily.
messages: Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).

224308:BSU 05990
gene id: 224308:BSU_05990
uniprot id: A0A6M3Z801
uniprot id: O05523
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis possesses two minimal, substrate-specific, Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a TatA and a TatC protein. TatA is bifunctional and performs the function of both the TatA and TatB proteins of Gram-negative organisms.
messages: Belongs to the TatC family.
messages: Cell membrane
messages: Forms a complex with TatA.
messages: Forms a complex with TatAy. Two types of complexes exist: one composed of TatAy and TatCy, and another composed only of TatAy.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Required for YwbN secretion.

224308:BSU 30160
gene id: 224308:BSU_30160
uniprot id: Q795R2
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Deletion of the gene has a negative impact in the utilization of polygalacturonan and rhamnogalacturonan type I.
messages: Involved in pectin degradation (PubMed:29240795). Part of the ABC transporter complex YtcQP-YteP involved in the uptake of polygalacturonan and rhamnogalacturonan type I (PubMed:29240795).
messages: The complex is probably composed of two ATP-binding proteins (MsmX), two transmembrane proteins (YtcP and YteP) and a solute-binding protein (YtcQ).

224308:BSU 38710
gene id: 224308:BSU_38710
uniprot id: A0A6M3ZIJ6
uniprot id: P94369
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the purine-cytosine permease (2.A.39) family.
messages: Cell membrane
messages: Membrane

224308:BSU 15430
gene id: 224308:BSU_15430
uniprot id: A0A6M3ZAS7
uniprot id: Q45477
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.
messages: Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
messages: Cytoplasm
messages: IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 29190
gene id: 224308:BSU_29190
uniprot id: A0A6M3ZEX6
uniprot id: O34529
names: Activity Regulation
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.
messages: Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.
messages: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
messages: Cytoplasm
messages: Homotetramer.
messages: Homotetramer. Component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno (PubMed:19193632) (although rnjA and rnjB's presence is unclear). Specifically interacts with RNase Y (rny, PubMed:21803996) and enolase (eno, PubMed:22198292). Interacts with BrxC (PubMed:33722570).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 14860
gene id: 224308:BSU_14860
uniprot id: A0A6M3ZAG6
uniprot id: Q9KWU4
names: Activity Regulation
names: Function
names: Induction
names: Subunit
messages: Activated by the cyclic di-AMP (c-di-AMP) receptor DarB in the absence of c-di-AMP (PubMed:35130724). Allosterically activated by acetyl-CoA (PubMed:4146915, PubMed:35130724). Inhibited by the biotin-complexing protein avidin (PubMed:4146915).
messages: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production (PubMed:4146915). Fulfills an anaplerotic function in B.subtilis as it is necessary for growth on glucose, but is not required for sporulation (PubMed:4146915).
messages: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
messages: Constitutively expressed.
messages: Homotetramer (Probable). At very low potassium concentrations, when intracellular levels of c-di-AMP are low, interacts with apo-DarB (PubMed:35130724). c-di-AMP inhibits the binding of DarB to PYC (PubMed:35130724). Does not bind directly c-di-AMP (PubMed:35130724).

224308:BSU 28680
gene id: 224308:BSU_28680
uniprot id: P94535
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
messages: Cell membrane
messages: Component of a complex that catalyzes the oxidation of glycolate to glyoxylate. Is also able to oxidize D-lactate ((R)-lactate). Does not link directly to O(2), and 2,6-dichloroindophenol (DCIP) and phenazine methosulfate (PMS) can act as artificial electron acceptors in vitro, but the physiological molecule that functions as primary electron acceptor during glycolate oxidation is unknown.
messages: The glycolate oxidase likely consists of several subunits including GlcD and GlcF.

224308:BSU 09958
gene id: 224308:BSU_09958
uniprot id: A0A6M4JRA6
uniprot id: C0H3Y2
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Expressed during the later stages of sporulation.
messages: Expression is regulated by the mother cell-specific transcription factors sigma K and GerE.
messages: Membrane
messages: Spore coat
messages: Spore protein involved in the assembly of several components of the spore coat, including CotB, CotG and CotH, and in spore germination.
messages: Spores of the null mutant show partial lack of assembly of several coat components, including CotB, CotG and CotH, and impairment of response during germination.

224308:BSU 13920
gene id: 224308:BSU_13920
uniprot id: P37955
names: Function
messages: Regulator of the spore photoproduct lyase operon (splAB).

224308:BSU 10370
gene id: 224308:BSU_10370
uniprot id: A0A6M4JID1
uniprot id: O07620
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the BioY family.
messages: Cell membrane
messages: Probable biotin transporter.
messages: Repressed by presence of biotin, under control of BirA. Probably part of the bioY-yhfST operon.

224308:BSU 27730
gene id: 224308:BSU_27730
uniprot id: A0A6M3ZEK7
uniprot id: O32055
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RuvB family.
messages: Cytoplasm
messages: Has 3 domains, the large (RuvB-L) and small ATPase (RuvB-S) domains and the C-terminal head (RuvB-H) domain. The head domain binds DNA, while the ATPase domains jointly bind ATP, ADP or are empty depending on the state of the subunit in the translocation cycle. During a single DNA translocation step the structure of each domain remains the same, but their relative positions change.
messages: Homohexamer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ (By similarity). Homohexamer which interacts with RecU (PubMed:19422832).
messages: Homohexamer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. RuvB forms 2 homohexamers on either side of HJ DNA bound by 1 or 2 RuvA tetramers; 4 subunits per hexamer contact DNA at a time. Coordinated motions by a converter formed by DNA-disengaged RuvB subunits stimulates ATP hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, pulling 2 nucleotides of DNA out of the RuvA tetramer per ATP hydrolyzed, thus driving DNA branch migration. The RuvB motors rotate together with the DNA substrate, which together with the progressing nucleotide cycle form the mechanistic basis for DNA recombination by continuous HJ branch migration. Branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves cruciform DNA.

224308:BSU 14710
gene id: 224308:BSU_14710
uniprot id: O07625
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 22640
gene id: 224308:BSU_22640
uniprot id: A3F3C7
uniprot id: P07600
names: Function
names: Similarity
names: Subunit
messages: Belongs to the TrpB family.
messages: Tetramer of two alpha and two beta chains.
messages: The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

224308:BSU 22720
gene id: 224308:BSU_22720
uniprot id: P31105
names: Function
names: Subunit
messages: Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP. CheR is responsible for the chemotactic adaptation to repellents.
messages: Monomer.

224308:BSU 39640
gene id: 224308:BSU_39640
uniprot id: A0A6M4JQU5
uniprot id: P42423
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Part of the ABC transporter complex YxdLM which could be involved in peptide resistance. Responsible for energy coupling to the transport system (Probable).
messages: The complex is composed of two ATP-binding proteins (YxdL) and two transmembrane proteins (YxdM).
messages: Transcriptionally regulated by YxdJ. Induced by the antibacterial protein LL-37, probably via YxdJ.

224308:BSU 19540
gene id: 224308:BSU_19540
uniprot id: O34844
names: Function
names: Induction
messages: Negatively regulates yodC and azoR1 which may contribute to the degradation of aromatic compounds. Probably positively regulates the catechol-specific transcription of mhqNOP, mhqED, and mhqA.
messages: Repressed by 2-methylhydroquinone (2-MHQ), diamide and catechol stress.

224308:BSU 11540
gene id: 224308:BSU_11540
uniprot id: O31605
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M3B family.
messages: Binds 1 zinc ion.

224308:BSU 08240
gene id: 224308:BSU_08240
uniprot id: A0A6M3Z8Y2
uniprot id: P54721
names: Function
names: Induction
names: Similarity
messages: Belongs to the extradiol ring-cleavage dioxygenase family.
messages: Involved in the meta cleavage of catechol to 2-hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol.
messages: Strongly induced by catechol, less strongly by 2-methylhydroquinone (2-MHQ) but only weakly by chromanon (6-brom-2-vinyl-chroman-4-on).

224308:BSU 10110
gene id: 224308:BSU_10110
uniprot id: P38050
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall.
messages: Expression remains constant during vegetative growth, decreases during early sporulation, and is induced in the forespore during late sporulation.
messages: In the C-terminal section; belongs to the transpeptidase family.
messages: In the N-terminal section; belongs to the glycosyltransferase 51 family.

224308:BSU 12620
gene id: 224308:BSU_12620
uniprot id: P54328
names: Similarity
messages: To B.subtilis YqbH.

224308:BSU 24680
gene id: 224308:BSU_24680
uniprot id: P25958
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Required for transformation and DNA binding.

224308:BSU 10480
gene id: 224308:BSU_10480
uniprot id: A0A6M3Z9J4
uniprot id: O07559
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TVP38/TMEM64 family.
messages: Cell membrane
messages: Membrane

224308:BSU 05820
gene id: 224308:BSU_05820
uniprot id: A0A6M3Z7X1
uniprot id: O05506
names: Cofactor
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Binds 1 Mg(2+) ion per trimer.
messages: Cytoplasm
messages: The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II GmuABC PTS system is involved in the transport of oligo-glucomannans such as cellobiose or mannobiose.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 31550
gene id: 224308:BSU_31550
uniprot id: O05253
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Part of an ABC transporter complex involved in the uptake of guanosine (PubMed:21926227). Responsible for energy coupling to the transport system (Probable). May be a nucleoside transporter of broad specificity but with various affinities for different substrates (PubMed:21926227).
messages: The complex is composed of two ATP-binding proteins (NupO), two transmembrane proteins (NupP and NupQ) and a solute-binding protein (NupN).
messages: Transcriptionally regulated by CodY.

224308:BSU 22240
gene id: 224308:BSU_22240
uniprot id: A0A6M3ZDE0
uniprot id: P50836
names: Similarity
messages: Belongs to the small heat shock protein (HSP20) family.

224308:BSU 19880
gene id: 224308:BSU_19880
uniprot id: O31867
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 21470
gene id: 224308:BSU_21470
uniprot id: A0A6M4JKV9
uniprot id: P68579
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. SunT family.
messages: Cell membrane
messages: Homodimer.
messages: Membrane
messages: SunT (TC 3.A.1.112.4) is required for production of the lantibiotic sublancin-168, probably by both processing the signal peptide and exporting the resulting mature lantibiotic.

224308:BSU 24080
gene id: 224308:BSU_24080
uniprot id: A0A6M3ZDN9
uniprot id: P54531
names: Function
names: Similarity
messages: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
messages: Catalyzes the reversible deamination of L-leucine to 4-methyl-2-oxopentanoate.

224308:BSU 13270
gene id: 224308:BSU_13270
uniprot id: O34551
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02830
gene id: 224308:BSU_02830
uniprot id: P80869
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Homotetramer.

224308:BSU 21570
gene id: 224308:BSU_21570
uniprot id: O31997
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the yokI-yokJ operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; not dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YokI (PubMed:34280190). Neutralizes the ability to inhibit growth of cognate toxin YokI upon expression in E.coli. Does not have immunity protein activity on other LXG toxins (PubMed:22200572).
messages: Probably interacts with cognate toxin YokI but not with other non-cognate toxins. The interaction inhibits the toxic activity of YokI (Probable).

224308:BSU 25370
gene id: 224308:BSU_25370
uniprot id: P54467
names: Disruption Phenotype
names: Subcellular Location Annotation
messages: Cell membrane
messages: No visible phenotype, no effects on FloA or FloT membrane rafts.

224308:BSU 39110
gene id: 224308:BSU_39110
uniprot id: A0A6M4JQQ3
uniprot id: P42305
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATPase activity is stimulated by interaction with RNA.
messages: Belongs to the DEAD box helicase family. DbpA subfamily.
messages: Contains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA.
messages: Contains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA. Undergoes a conformation change in the helicase core upon binding of RNA and ATP.
messages: Cytoplasm
messages: DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes (Probable).
messages: DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes.
messages: In rich medium highest expression in exponential growth, expression decreases in stationary phase (at protein level).
messages: May interact with RNA helicases CshA and CshB.
messages: No visible effect at 37 or 16 degrees Celsius; no change in ribosome profiles. A quadruple disruption of all RNA helicases (cshA, cshB, deaD, yfmL) is not lethal at 37 degrees Celsius, although both 50S and 70S ribosomes are decreased, while growth stops at 16 degrees.

224308:BSU 04360
gene id: 224308:BSU_04360
uniprot id: A0A6M4JDE8
uniprot id: P96593
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NRAMP family.
messages: Cell membrane
messages: H(+)-stimulated, divalent metal cation uptake system.
messages: H(+)-stimulated, divalent metal cation uptake system. Involved in manganese uptake. Can probably also transport cadmium, cobalt, copper and zinc, but not iron. May be the predominant transporter of manganese during logarithmic phase growth.
messages: Repressed by MntR in the presence of manganese.

224308:BSU 29670
gene id: 224308:BSU_29670
uniprot id: P22326
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.
messages: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
messages: Cytoplasm
messages: Expressed during vegetative growth.
messages: Homodimer.

224308:BSU 14390
gene id: 224308:BSU_14390
uniprot id: A0A6M3ZDU3
uniprot id: O31714
names: Function
names: Similarity
messages: Belongs to the carbohydrate kinase PfkB family.
messages: Catalyzes the ATP-dependent phosphorylation of fructose-l-phosphate to fructose-l,6-bisphosphate.

224308:BSU 21820
gene id: 224308:BSU_21820
uniprot id: A0A6M4JIA1
uniprot id: P11044
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.
messages: Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
messages: Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 33460
gene id: 224308:BSU_33460
uniprot id: A0A6M3ZGD1
uniprot id: O32216
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0126 family.
messages: Cell membrane
messages: Membrane

224308:BSU 19340
gene id: 224308:BSU_19340
uniprot id: A0A6M4JN95
uniprot id: O34383
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.
messages: Cell membrane
messages: Membrane
messages: Putative sodium-dependent transporter.

224308:BSU 08860
gene id: 224308:BSU_08860
uniprot id: A0A6M4JGP3
uniprot id: P40402
names: Function
names: Induction
names: Similarity
messages: Belongs to the SsuD family.
messages: Catalyzes the desulfonation of aliphatic sulfonates.
messages: Repressed by sulfate or cysteine.

224308:BSU 40890
gene id: 224308:BSU_40890
uniprot id: A0A6M3ZI75
uniprot id: P21475
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bS18 family.
messages: Binds as a heterodimer with protein bS6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Forms a tight heterodimer with protein bS6 (By similarity).
messages: Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein bS6.
messages: Strongly expressed during exponential growth, decreases 2-4-fold in stationary phase, part of the rpsF-ssbA-rpsR operon (PubMed:14762004). The operon is induced by DNA damage by mitomycin C (PubMed:14762004).

224308:BSU 02160
gene id: 224308:BSU_02160
uniprot id: O31443
names: Function
messages: Putative DNA-binding acetyltransferase.

224308:BSU 34790
gene id: 224308:BSU_34790
uniprot id: A0A6M3ZL10
uniprot id: P80880
names: Cofactor
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.
messages: Binds 1 FAD per subunit.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Cytoplasm
messages: Homodimer.
messages: The active site is a redox-active disulfide bond.

224308:BSU 30550
gene id: 224308:BSU_30550
uniprot id: A0A6M4JMM6
uniprot id: P54419
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the AdoMet synthase family.
messages: Binds 1 potassium ion per subunit.
messages: Binds 2 divalent ions per subunit.
messages: Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.
messages: Cytoplasm
messages: Homotetramer; dimer of dimers.

224308:BSU 39810
gene id: 224308:BSU_39810
uniprot id: A0A6M3ZI04
uniprot id: P46333
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Could serve either a nutritional or an osmotic protection function.
messages: Membrane

224308:BSU 16050
gene id: 224308:BSU_16050
uniprot id: A0A6M3ZAQ9
uniprot id: O31743
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.
messages: Cytoplasm
messages: Essential for growth, it cannot be disrupted. In depletion experiments cells become over 3-fold longer, are abnormally curved and nucleoids condense.
messages: Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.
messages: Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins rplP and rpmA with the large subunit.
messages: Estimated to be present at 1000 copies per cell.
messages: Interacts with ctc. Interacts with the immature 50S ribosome subunit. 2 molecules of RbgA bind to one 50S subunit.
messages: Interacts with ctc. Interacts with the immature 50S ribosome subunit. 2 molecules of rbgA bind to one 50S subunit.
messages: Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.

224308:BSU 13120
gene id: 224308:BSU_13120
uniprot id: A0A6M4JHC5
uniprot id: P39820
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glutamate 5-kinase family.
messages: Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.
messages: Cytoplasm

224308:BSU 10220
gene id: 224308:BSU_10220
uniprot id: A0A6M4JGP2
uniprot id: O07605
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.
messages: Cell membrane
messages: Homotrimer (By similarity). Interacts with FloT (PubMed:23651456).
messages: In the presence of glutamate in the medium, the expression is reduced two-fold, at high potassium concentration (5 mM). In the absence of glutamate, the expression is about two-fold increased at the increased potassium concentration.
messages: Membrane
messages: Membrane raft
messages: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system.

224308:BSU 12240
gene id: 224308:BSU_12240
uniprot id: A0A6M4JH75
uniprot id: O34684
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0014 family.
messages: Cell membrane
messages: Membrane

224308:BSU 17670
gene id: 224308:BSU_17670
uniprot id: O31802
names: Similarity
messages: To B.subtilis spore coat protein C.

224308:BSU 05800
gene id: 224308:BSU_05800
uniprot id: A0A6M3Z811
uniprot id: Q797E3
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily.
messages: Cell membrane
messages: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6-diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role in maintaining the cellular purine base pools and protecting cells against toxic purine base analogs. Modulates expression of the purR and G-box regulons.
messages: Membrane
messages: Up-regulated by adenine, via the adenine-dependent riboswitch. Also up-regulated by hypoxanthine and guanine.

224308:BSU 32250
gene id: 224308:BSU_32250
uniprot id: A0A6M4JRK9
uniprot id: P04990
names: Function
names: Similarity
messages: Belongs to the threonine synthase family.
messages: Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.

224308:BSU 15960
gene id: 224308:BSU_15960
uniprot id: O31737
names: Induction
names: Subcellular Location Annotation
messages: Secreted
messages: Transcriptionally regulated by SigD.

224308:BSU 17610
gene id: 224308:BSU_17610
uniprot id: P39211
names: Function
names: Similarity
messages: Belongs to the FGGY kinase family.
messages: Catalyzes the phosphorylation of D-xylulose to D-xylulose 5-phosphate.

224308:BSU 00920
gene id: 224308:BSU_00920
uniprot id: A0A6M4JC62
uniprot id: P22250
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
messages: Cytoplasm
messages: Monomer.

224308:BSU 11250
gene id: 224308:BSU_11250
uniprot id: A0A6M3Z9C7
uniprot id: P18186
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.
messages: Cytoplasm
messages: Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.

224308:BSU 25620
gene id: 224308:BSU_25620
uniprot id: A0A6M3ZHD5
uniprot id: P54457
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Iojap/RsfS family.
messages: Cytoplasm
messages: Functions as a ribosomal silencing factor. Interacts with ribosomal protein uL14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.
messages: Interacts with ribosomal protein uL14 (rplN).

224308:BSU 23890
gene id: 224308:BSU_23890
uniprot id: A0A6M4JJ39
uniprot id: P54544
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the OXA1/ALB3/YidC family. Type 2 subfamily.
messages: Cell membrane
messages: Disruption decreases genetic competence by about 50%, significant loss of expression of ComGC, no effect on sporulation. A double spoIIIJ-yqjG deletion is lethal.
messages: Membrane
messages: Mostly monomeric, it may also form dimers. Interacts with SpoIIIAE. Forms a complex with the F(1)F(0) ATP synthase in which can be found the alpha, beta, gamma, delta and epsilon subunits of F(1) and a, b and subunits of F(0). YqgA is found in the same complex.
messages: Predominantly expressed in vegetative cells.
messages: Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity). Also involved in protein secretion processes. It has an overlapping, although partly distinct, function compared to SpoIIIJ(MisCB).
messages: Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins.

224308:BSU 30760
gene id: 224308:BSU_30760
uniprot id: O34338
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Probably part of the ABC transporter complex MntABCD involved in manganese import. Probably responsible for energy coupling to the transport system.
messages: Repressed by MntR in the presence of manganese.
messages: The complex is probably composed of two ATP-binding proteins (MntB), two transmembrane proteins (MntC and MntD) and a solute-binding protein (MntA).

224308:BSU 17960
gene id: 224308:BSU_17960
uniprot id: P45711
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Interacts with the N-terminal D1 domain of dynamin-like protein DynA.

224308:BSU 22650
gene id: 224308:BSU_22650
uniprot id: A3F3C8
uniprot id: P20167
names: Similarity
messages: Belongs to the TrpF family.

224308:BSU 11950
gene id: 224308:BSU_11950
uniprot id: O31639
names: Induction
messages: Expression is sigma D-dependent.

224308:BSU 10910
gene id: 224308:BSU_10910
uniprot id: A0A6M4JF37
uniprot id: O06735
names: Function
names: Similarity
messages: Belongs to the APS kinase family.
messages: Catalyzes the synthesis of activated sulfate.

224308:BSU 35800
gene id: 224308:BSU_35800
uniprot id: A0A6M3ZH62
uniprot id: P39569
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Involved in the response to the germinative mixture of L-asparagine, glucose, fructose and potassium ions (AGFK). Cannot stimulate germination in the absence of gerD and gerK gene products (fructose and glucose receptors respectively).
messages: Membrane

224308:BSU 33700
gene id: 224308:BSU_33700
uniprot id: A0A6M3ZGP8
uniprot id: P39775
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for choline; probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Repressed by GbsR.

224308:BSU 17550
gene id: 224308:BSU_17550
uniprot id: P94485
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30220
gene id: 224308:BSU_30220
uniprot id: A0A6M3ZFQ5
uniprot id: P53556
names: Function
names: Similarity
names: Subunit
messages: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.
messages: Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.
messages: Homodimer.

224308:BSU 05600
gene id: 224308:BSU_05600
uniprot id: P96703
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 16730
gene id: 224308:BSU_16730
uniprot id: Q04809
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Dipicolinate synthase likely consists of DpaA and DpaB, since both proteins are required for DPA synthesis.
messages: Disruption of this gene has no effect on vegetative growth but causes a sporulation defect, characterized by heat-sensitive spores devoid of DPA, which can be cured by supplementation with dipicolinate.
messages: Is not expressed during vegetative growth; is expressed at stage 5 of sporulation specifically in the mother cell compartment, under the control of the sigma-K factor. Is repressed by GerE.
messages: Together with DpaB, catalyzes the conversion of dihydrodipicolinate to dipicolinate (DPA), which constitutes up to 10% of the dry weight of the spore.

224308:BSU 16250
gene id: 224308:BSU_16250
uniprot id: A0A6M4JI87
uniprot id: P20487
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FliJ family.
messages: Cell membrane
messages: Flagellar protein that affects chemotactic events.
messages: Membrane

224308:BSU 30520
gene id: 224308:BSU_30520
uniprot id: O34696
names: Similarity
messages: Belongs to the transferase hexapeptide repeat family.

224308:BSU 08630
gene id: 224308:BSU_08630
uniprot id: A0A6M4JEA0
uniprot id: O31584
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Adenine glycosylase active on G-A mispairs.
messages: Base excision repair (BER) glycosylase that initiates repair of A:oxoG to C:G by removing the inappropriately paired adenine base from the DNA backbone, generating an abasic site product. 8-oxoguanine (oxoG) is a genotoxic DNA lesion resulting from oxidation of guanine; this residue is misread by replicative DNA polymerases, that insert adenine instead of cytosine opposite the oxidized damaged base. Shows a powerful dicrimination of A versus C, since it does not cleave cytosine in oxoG:C pairs. May also be able to remove adenine from A:G mispairs, although this activity may not be physiologically relevant.
messages: Belongs to the Nth/MutY family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Binds 1 [4Fe-4S] cluster. The cluster has a structural role.
messages: Cells lacking this gene have a 100-fold increased spontaneous mutation frequency. A double mutM/mutY disruption has a 1000-fold increased spontaneous mutation frequency (PubMed:12483591). Triple ytkD/mutM/mutY disrupted strains show increased mutation rates during exponential and stationary phase (PubMed:19011023).
messages: Expressed during exponential growth. Expressed as both a monocistronic transcript and a mutY-fabL-sspE transcript.

224308:BSU 09180
gene id: 224308:BSU_09180
uniprot id: A0A6M4JGB8
uniprot id: P54601
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Spore coat

224308:BSU 28150
gene id: 224308:BSU_28150
uniprot id: A0A6M4JK18
uniprot id: P16616
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the HMBS family.
messages: Binds 1 dipyrromethane group covalently.
messages: Monomer.
messages: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
messages: The porphobilinogen subunits are added to the dipyrromethane group.

224308:BSU 29260
gene id: 224308:BSU_29260
uniprot id: O34922
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39720
gene id: 224308:BSU_39720
uniprot id: A0A6M3ZKG5
uniprot id: P42416
names: Function
names: Induction
names: Similarity
messages: Belongs to the IolE/MocC family.
messages: By inositol.
messages: Catalyzes the dehydration of inosose (2-keto-myo-inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).

224308:BSU 19650
gene id: 224308:BSU_19650
uniprot id: O34349
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PA-phosphatase related phosphoesterase family.
messages: Catalyzes the dephosphorylation of phosphatidylglycerophosphate (PGP) to phosphatidylglycerol. Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate.
messages: Cell membrane

224308:BSU 10820
gene id: 224308:BSU_10820
uniprot id: O07940
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
messages: Cell membrane

224308:BSU 00290
gene id: 224308:BSU_00290
uniprot id: P37538
names: Disruption Phenotype
names: Domain
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Binds cyclic di-AMP (c-di-AMP) and is probably involved in c-di-AMP-mediated signaling pathways. In vitro, can also bind cyclic GMP-AMP (3'3'-cGAMP), with lower affinity, but not c-di-GMP or 2'3'-cGAMP.
messages: Binds three molecules of c-di-AMP, each in a pocket located between two subunits. c-di-AMP binding is accompanied by conformational changes of both the fold and the position of the B-loop.
messages: Cytoplasm
messages: Homotrimer.
messages: Not essential.

224308:BSU 19760
gene id: 224308:BSU_19760
uniprot id: P42092
names: Function
names: Similarity
messages: May be involved in maturation of the outermost layer of the spore.
messages: To B.subtilis spore coat polysaccharide biosynthesis protein SpsA.

224308:BSU 39540
gene id: 224308:BSU_39540
uniprot id: A0A6M4JQT3
uniprot id: P54948
names: Similarity
messages: Belongs to the peptidase C59 family.

224308:BSU 27920
gene id: 224308:BSU_27920
uniprot id: A0A6M4JMW3
uniprot id: P20964
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A mutant in the N-terminal obg domain (Asp-92) impairs growth and ribosome association but has no effect on sporulation or the general stress regulon (GSR). Replacing the last 22 amino acids has no effect on growth or ribosome association, but eliminates sporulation and reduces the GSR, showing for the first time that growth promotion and the GSR phenotypes are separable.
messages: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.
messages: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.
messages: Cytoplasm
messages: Essential for growth, it cannot be disrupted. In depletion experiments cells become over 3-fold longer, are abnormally curved and nucleoids condense.
messages: Estimated to be present at 6000 copies per cell.
messages: Inhibited by GDP; less than 20 uM ppGpp stimulates the GTPase, while higher concentrations inhibit.
messages: Monomer.
messages: Monomer. Interacts with TasA (AC P54507) in pull-down experiments.
messages: Necessary for the transition from vegetative growth to stage 0 or stage II of sporulation, but sporulation subsequent to these stages is unaffected at 45 degrees Celsius. This ts effect is probably due solely to the E-79 mutation. Required for expression of early sporulation genes, further suggesting a role in the induction of sporulation. Depletion effects on sporulation can be partially suppressed by missense mutations in spo0A. Strains depleted for obg stop growing after about 3 hours and do not induce the sigma-B factor following ethanol stress. It cofractionates with the ribosome and upstream stress response regulators RsbR, RsbS and RsbT in size fractionation columns, suggesting the ribosome might serve as a possible mediator of the activity of obg and the stress induction of sigma-B. In glycerol gradients partially associates with ribosomes; this is stabilized by a nonhydrolyzable GTP-analog and to a lesser extent GTP and GDP.
messages: Part of an operon with spo0B.

224308:BSU 23980
gene id: 224308:BSU_23980
uniprot id: A0A6M3ZDM8
uniprot id: P54535
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Cells show impaired growth on arginine as the nitrogen source.
messages: Membrane
messages: Part of a binding-protein-dependent transport system for arginine.

224308:BSU 36590
gene id: 224308:BSU_36590
uniprot id: A0A6M3ZLA7
uniprot id: P71040
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phospholipase D family. Cardiolipin synthase subfamily.
messages: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol.
messages: Cell membrane
messages: Cells lacking this gene show a lack of cardiolipin (PubMed:14973018). Cells appear normal, no effect on flotillin cluster numbers or size (PubMed:27362352).
messages: Membrane

224308:BSU 20420
gene id: 224308:BSU_20420
uniprot id: P68575
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: In response to low temperature and salt stress.

224308:BSU 16130
gene id: 224308:BSU_16130
uniprot id: A0A6M3ZB43
uniprot id: P39815
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MnmG family. TrmFO subfamily.
messages: Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 37610
gene id: 224308:BSU_37610
uniprot id: A0A6M4JQ90
uniprot id: O32280
names: Similarity
messages: Belongs to the UPF0741 family.

224308:BSU 11100
gene id: 224308:BSU_11100
uniprot id: A0A6M4JIK0
uniprot id: P39899
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M4 family.
messages: Binds 1 zinc ion per subunit.
messages: Cells lacking this gene grow normally in both LB and SG media, and no difference in sporulation frequency can be observed.
messages: Extracellular zinc metalloprotease.
messages: Protease able to cleave casein in vitro.
messages: Protease activity can be inhibited in vitro by either a zinc specific chelator, 1,10-phenanthroline, or a metal chelator, EDTA. The enzyme is resistant to phenylmethylsulfonyl fluoride and iodoacetic acid.
messages: Secreted

224308:BSU 10860
gene id: 224308:BSU_10860
uniprot id: A0A6M3Z998
uniprot id: O07939
names: Similarity
messages: Belongs to the DinB family.

224308:BSU 15300
gene id: 224308:BSU_15300
uniprot id: A0A6M4JLM2
uniprot id: P16397
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S8 family.
messages: Secreted

224308:BSU 09840
gene id: 224308:BSU_09840
uniprot id: Q796V8
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Although the enzyme shows to be able to complement hemF hemN double mutant and to accumulate coproporphyrinogen-III when the gene is disrupted, no oxygen-independent coproporphyrinogen-III oxidase activity has been shown. The exact role of this protein is still unknown.
messages: Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemZ subfamily.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Cells lacking this gene accumulate coproporphyrin-III only under anaerobic conditions, however no obvious respiratory growth phenotype is observed under anaerobic conditions. The hemN hemZ double mutant do not abolish aerobic and anaerobic respiratory growth, however a reduction of growth is observed.
messages: HemZ is able to complement a S.typhimurium hemF hemN double mutant under aerobic and anaerobic conditions, however under anaerobic growth conditions, the double mutant complemented with hemZ grows significantly faster.
messages: Induced under anaerobic conditions and by hydrogen peroxide stress. It is also dependent on induction by ResDE, Fnr and ArfM.
messages: Involved in the biosynthesis of porphyrin-containing compound.

224308:BSU 16750
gene id: 224308:BSU_16750
uniprot id: A0A6M3ZB82
uniprot id: Q04797
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aspartate-semialdehyde dehydrogenase family.
messages: Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 29760
gene id: 224308:BSU_29760
uniprot id: P39914
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Cells lacking the operon including this gene (ytxGH-brxC) have increased S-bacillithiolation of glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) GapA and GapB in cells with increased basal levels of oxidative stress due to concomitant deletion of genes encoding for catalase (katA) and alkyl hydroperoxide reductase (ahpCF).
messages: Cys can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation protects Cys residues against overoxidation by acting as a redox switch in response to oxidative stress.
messages: Interacts with AbrB, BdhA, Bdr, BrxB, FolD, GapA, GapB, GatA, PfkA, PyrAA, PyrAB, PyrE, PyrG, PyrH, RpsB, RpsK, RpsL, SalA, SucC, Tuf and YtsJ.
messages: S-bacillithiolation is the formation of mixed disulfide bonds between protein thiols and the general thiol reductant bacillithiol (BSH) under oxidative stress. BSH is an equivalent of glutathione (GSH) in Firmicutes. This protein is a monothiol bacilliredoxin, which debacillithiolates (removes BSH) the S-bacillithiolated glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) GapA and GapB in vivo and probably a number of other oxidized cytosolic proteins. Debacillithiolates the S-bacillithiolated Bdr (Bdr-SSB) and BrxB (BrxB-SSB) in vitro. Involved in maintaining redox homeostasis in response to disulfide stress conditions.

224308:BSU 00640
gene id: 224308:BSU_00640
uniprot id: P37475
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation.

224308:BSU 30330
gene id: 224308:BSU_30330
uniprot id: O34881
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 12880
gene id: 224308:BSU_12880
uniprot id: A0A6M3ZDC6
uniprot id: O34575
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 39 family.
messages: Cell membrane
messages: Membrane

224308:BSU 10360
gene id: 224308:BSU_10360
uniprot id: O07619
names: Function
names: Induction
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: May be involved in fatty acid metabolism.
messages: Repressed by presence of biotin, under control of BirA. Probably part of the bioY-yhfST operon.

224308:BSU 23590
gene id: 224308:BSU_23590
uniprot id: Q07683
names: Function
messages: Transcriptional repressor for the ans operon coding for L-asparaginase and L-aspartase. NH4(+) may influence this repression.

224308:BSU 04110
gene id: 224308:BSU_04110
uniprot id: P42969
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 'GDSL' lipolytic enzyme family.
messages: Lipase involved in spore germination.
messages: Spore coat
messages: Transcribed from stage t4 of sporulation. Expressed in the mother cell compartment at the late stage of sporulation and localizes around forespores in a CotE, SafA and SpoIVD-dependent manner.
messages: Transcribed under the control of SigK and positively regulated by GerE.

224308:BSU 13980
gene id: 224308:BSU_13980
uniprot id: A0A6M4JHK5
uniprot id: Q796K8
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: Increases progressively during log phase, peaking at stationary phase of vegetative growth.
messages: Involved in the polymerization of peptidoglycan. Plays a redundant role with PBP-2A (pbpA) in determining the rod shape of the cell during vegetative growth and spore outgrowth.
messages: It is uncertain whether Met-1 or Met-20 is the initiator; if Met-20 then the protein would probably be secreted.
messages: No visible phenotype during vegetative growth, sporulation or spore germination. No change in antibiotic resistance. Double pbpA-pbpH mutants cannot be made, suggesting the 2 proteins have redundant, essential roles in vegetative growth. Depletion of PbpH in the pbpA deletion leads to cell growth arrest after 3 generations; cells swell, round-up, many lyse and division septa are very irregular. Germinated spore are spherical and eventually lyse.

224308:BSU 25130
gene id: 224308:BSU_25130
uniprot id: A0A6M4JNG3
uniprot id: P54476
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the AP endonuclease 2 family.
messages: Binds 3 Zn(2+) ions.
messages: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.

224308:BSU 36640
gene id: 224308:BSU_36640
uniprot id: A0A6M4JPZ2
uniprot id: P77837
names: Cofactor
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.
messages: Binds 2 nickel ions per subunit.
messages: Carbamylation allows a single lysine to coordinate two nickel ions.
messages: Carboxylation allows a single lysine to coordinate two nickel ions.
messages: Cytoplasm
messages: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.

224308:BSU 11760
gene id: 224308:BSU_11760
uniprot id: Q08313
names: Subcellular Location Annotation
messages: Spore coat

224308:BSU 35750
gene id: 224308:BSU_35750
uniprot id: A0A6M3ZH91
uniprot id: P27620
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 26 family. TagA/TarA subfamily.
messages: Catalyzes the conversion of GlcNAc-PP-undecaprenol into ManNAc-GlcNAc-PP-undecaprenol, the first committed lipid intermediate in the de novo synthesis of teichoic acid.
messages: Not essential. Impaired growth. Looses rod shape of cells. No teichoic acid in cell walls.
messages: Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.

224308:BSU 38570
gene id: 224308:BSU_38570
uniprot id: A0A6M4JQJ7
uniprot id: P46319
names: Cofactor
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Binds 1 Mg(2+) ion per trimer.
messages: Cytoplasm
messages: Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lichenan transport.

224308:BSU 36750
gene id: 224308:BSU_36750
uniprot id: P07372
names: Function
messages: May act at the level of sigma-G activity or its stability. SpoIID is probably required for engulfment.

224308:BSU 29370
gene id: 224308:BSU_29370
uniprot id: A0A6M3ZEZ4
uniprot id: O34852
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.
messages: The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).

224308:BSU 12840
gene id: 224308:BSU_12840
uniprot id: A0A6M4JMA1
uniprot id: O34436
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily.
messages: Cell membrane
messages: Low-affinity inorganic phosphate transporter.
messages: Membrane

224308:BSU 34430
gene id: 224308:BSU_34430
uniprot id: A0A6M3ZGH8
uniprot id: P32960
names: Developmental Stage
names: Function
names: Similarity
names: Subunit
messages: Amino-acid racemase able to utilize a broad range of substrates. Preferentially catalyzes the epimerization of LL-diaminopimelate, as well as the racemization of D-lysine, L-arginine, L-ornithine, L-lysine and D-arginine. Has lower activity against D-ornithine, L-histidine, L-alanine, L-tyrosine, L-phenylalanine, L-serine, L-glutamine, L-methionine, L-asparagine and L-homoserine. Has weak activity against L-norleucine, L-aminobutyric acid and L-norvaline. Has no activity toward nine L-amino acids (Thr, Glu, Asp, Val, Leu, Ile, Trp, Cit and Aad) (PubMed:28894939). D-amino acids might be used as components of peptidoglycan and/or be involved in peptidoglycan metabolism and remodeling (PubMed:8491712, PubMed:28894939).
messages: Belongs to the aspartate/glutamate racemases family.
messages: Homodimer.
messages: Weakly expressed during vegetative growth and significantly induced at the onset of sporulation.

224308:BSU 17990
gene id: 224308:BSU_17990
uniprot id: A0A6M3ZM49
uniprot id: P71031
names: Caution
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspO family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Expression is sigma G-dependent.
messages: Spore core
messages: Was originally thought to be a spore coat protein.

224308:BSU 28050
gene id: 224308:BSU_28050
uniprot id: A0A6M4JK08
uniprot id: Q02169
names: Caution
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Maf family. YhdE subfamily.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Not essential for cell division.
messages: Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. Can also hydrolyze CTP and the modified nucleotides pseudo-UTP, 5-methyl-CTP (m(5)CTP) and 5-methyl-UTP (m(5)UTP) (PubMed:24210219). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids (PubMed:24210219).
messages: Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.
messages: Overexpression results in extensive filamentation caused by a disruption and subsequent inhibition of the septation process.

224308:BSU 27260
gene id: 224308:BSU_27260
uniprot id: O05393
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the cysteine synthase/cystathionine beta-synthase family.
messages: By methionine. Repressed by sulfate and cysteine.
messages: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine.
messages: Grows very slowly with methionine as sole sulfur source.

224308:BSU 24730
gene id: 224308:BSU_24730
uniprot id: A0A6M3ZEE5
uniprot id: P25953
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GSP E family.
messages: Cell membrane
messages: Expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:11918817, PubMed:16009133, PubMed:17630974).
messages: Expression activated by ComK (PubMed:11918817, PubMed:11948146).
messages: Required for uptake of DNA by competent cells.
messages: Transformation deficient (PubMed:16009133).

224308:BSU 09520
gene id: 224308:BSU_09520
uniprot id: A0A6M3ZE71
uniprot id: O07582
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: Forms an operon with yhdL and yhdK, maximally expressed during early to mid-exponential growth phases (PubMed:10216858). Transcription decreases during late exponential growth; positively autoregulated (PubMed:10216858). Negatively regulated by yhdL and yhdK (PubMed:10216858). Induced by 5% ethanol, 80 uM paraquat, pH 4.3, the antibiotics, inhibitors of cell wall, bacitracin, vancomycin and phosphomycin, heat shock of 10 minutes at 50 degrees Celsius (PubMed:12775685). No induction was observed with pH 9, H(2)O(2), monensin, and the detergents Triton X-100 and Tween 20 (PubMed:12775685). Induced 3-fold by the beta-lactam antibiotic cefuroxime (PubMed:22211522).
messages: Interacts with the N-terminus of YhdL, which is probably its anti-sigma factor (PubMed:14993308). Interacts transiently with the RNAP core (Probable).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by a cognate anti-sigma factor (YhdL) until released (PubMed:14993308). This sigma factor is involved in the maintenance of membrane and cell wall integrity in response to environmental stresses including salt, acid, ethanol and antibiotics stress (PubMed:22211522, PubMed:12775685). Partially regulates transcription from a number of genes including disA (PubMed:17434969). Associates with RNAP core under all growth phases (PubMed:21710567).
messages: Strains insertionally inactivated in the sigM gene form aberrantly shaped cells that swell and lyse in medium containing high levels of different salts (PubMed:10216858). 70-fold increased sensitivity to the beta-lactam antibiotic cefuroxime (CEF), double sigM-sigX mutants have 130-fold increased sensitivity to CEF (PubMed:22211522). Also sensitive to other antibiotics (PubMed:22211522).

224308:BSU 33130
gene id: 224308:BSU_33130
uniprot id: O32202
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Induced, via the two-component regulatory system LiaS/LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.

224308:BSU 03890
gene id: 224308:BSU_03890
uniprot id: A0A6M4JFH9
uniprot id: P94426
names: Function
names: Similarity
messages: Activates the transcription of the gabTD operon. Is also a repressor of its own expression, both in the presence and absence of GABA. Binds specifically to the DNA region overlapping the -35 region of the gabT promoter and the -10 and +1 regions of the gabR promoter. Principally regulates the utilization of gamma-aminobutyrate.
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 16440
gene id: 224308:BSU_16440
uniprot id: P39802
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Chemotaxis is substantially impaired, with a 30-fold reduction in the efficiency of chemotaxis with aspartate and 11-fold reduction with 2-deoxyglucose. Inactivation of cheW appears to have a subtle effect on chemotactic behavior in the absence of chemoeffectors: the bacteria display a smooth swimming bias, with some tumbling. A double mutant lacking both CheV and CheW has a strong tumble bias, does not respond to addition of attractant, shows essentially no accumulation in capillary assays, and has greatly reduced methyl turnover on the methyl-accepting chemotaxis proteins (MCPs).
messages: Cytoplasm
messages: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheV and CheW are involved in the coupling of the methyl-accepting chemoreceptors to the central two-component kinase CheA; they are both necessary for efficient chemotaxis.

224308:BSU 23570
gene id: 224308:BSU_23570
uniprot id: A0A6M3ZGX2
uniprot id: P26899
names: Similarity
names: Subunit
messages: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.
messages: Homotetramer.

224308:BSU 21940
gene id: 224308:BSU_21940
uniprot id: P68731
names: Function
names: Induction
names: Subunit
messages: Expression is sigma D-dependent. Negatively regulated by ComK.
messages: Homotetramer.
messages: Stabilizes the phosphorylated form of DegU, leading to enhanced production of levansucrase, alkaline protease, and neutral protease.

224308:BSU 24530
gene id: 224308:BSU_24530
uniprot id: A0A6M4JJ11
uniprot id: P54511
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the octanoyltransferase LipM family.
messages: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation.
messages: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation. Is also able to catalyze the reverse reaction. Octanoyl-CoA can also act as a substrate although very poorly. Does not display lipoate protein ligase activity, despite its sequence similarity to LplA.
messages: Cells lacking this gene are auxotrophic for lipoic acid when grown in minimal medium but grow as well as the wild-type strain in the presence of lipoic acid. The requirement for lipoic acid can be bypassed by addition of both acetate and branched-chain fatty acid (BCFA) precursors. The mutant cells are devoid of lipoylated proteins.
messages: In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. The reaction proceeds via an octanoyl-thioester enzyme intermediate.
messages: Monomer.

224308:BSU 11520
gene id: 224308:BSU_11520
uniprot id: A0A6M4JF94
uniprot id: P37958
names: Developmental Stage
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MecA family.
messages: Enables the recognition and targeting of unfolded and aggregated proteins to the ClpC protease or to other proteins involved in proteolysis. Acts negatively in the development of competence by binding ComK and recruiting it to the ClpCP protease. When overexpressed, inhibits sporulation. Also involved in Spx degradation by ClpC.
messages: Expressed throughout growth. There is a slight decline of expression during exponential growth and a slight increase after T0.
messages: Homodimer.
messages: The N-terminal domain has binding sites for ComK and ComS and probably for unfolded/aggregated proteins; the C-terminal domain interacts with ClpC.
messages: The N-terminal domain has binding sites for ComK and probably for unfolded/aggregated proteins; the C-terminal domain interacts with ClpC.

224308:BSU 23550
gene id: 224308:BSU_23550
uniprot id: A0A6M3ZDC1
uniprot id: P54572
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the malic enzymes family.
messages: Catalyzes the decarboxylation of malate to pyruvate. Is specific for NAD, cannot use NADP. Can also catalyze the decarboxylation of oxaloacetate (PubMed:16788182). Involved in keeping the ATP levels high (PubMed:23136871).
messages: Divalent metal cations.
messages: Divalent metal cations. Prefers magnesium or manganese.
messages: Mutant can use either a gluconeogenic carbon source or glucose as efficiently as the wild-type strain (PubMed:16788182). The ATP concentrations in the mutant grown in minimal medium with glucose are similar to the wild-type level. ATP concentrations slightly decrease in malate minimal medium. The mleA-maeA-malS triple mutant shows a decrease in ATP concentrations by about 20% and a moderate growth defect (PubMed:23136871). NADPH overproduction is roughly halved in the deletion mutant (PubMed:33824210).
messages: Weakly expressed in glucose or malate minimal medium.

224308:BSU 12730
gene id: 224308:BSU_12730
uniprot id: P54339
names: Similarity
messages: Belongs to the Mu gp47/PBSX XkdT family.

224308:BSU 10300
gene id: 224308:BSU_10300
uniprot id: A0A6M4JK64
uniprot id: P04189
names: Cofactor
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S8 family.
messages: Binds 2 calcium ions per subunit.
messages: Secreted
messages: Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.
messages: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
messages: The propeptide functions as an intramolecular chaperone which is essential for the correct folding of the protease domain but is not required for enzymatic function of the folded protein. It is autoprocessed and degraded after completion of the folding process.

224308:BSU 40700
gene id: 224308:BSU_40700
uniprot id: P37502
names: Cofactor
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 14350
gene id: 224308:BSU_14350
uniprot id: A0A6M3ZA91
uniprot id: O31710
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.
messages: Cell membrane
messages: Expressed in exponential-phase cells.
messages: Part of a complex composed of YknX, YknY and YknZ. The complex interacts with YknW.
messages: Part of an unusual four-component transporter, which is required for protection against the killing factor SdpC (sporulation-delaying protein).

224308:BSU 26030
gene id: 224308:BSU_26030
uniprot id: P45931
names: Similarity
messages: To B.subtilis XkdO.

224308:BSU 07450
gene id: 224308:BSU_07450
uniprot id: O34812
names: Function
names: Induction
names: Similarity
messages: Belongs to the NADP-dependent oxidoreductase L4BD family.
messages: Putative quinone oxidoreductase that may contribute to the degradation of aromatic compounds.
messages: induced by stress due to exposure to 2-methylhydroquinone (2-MHQ).

224308:BSU 22760
gene id: 224308:BSU_22760
uniprot id: P31112
names: Function
names: Subunit
messages: Heterodimer of component I and II.
messages: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7).

224308:BSU 33029
gene id: 224308:BSU_33029
uniprot id: O32302
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Deletion of the gene causes a decrease in the overall Spx levels, as well as a change in the dynamics of Spx accumulation.
messages: Induced under cell wall stress, but not disulfide stress, and this induction requires the CssRS two-component system, which responds to both secretion stress and cell wall antibiotics. Phosphorylated CssR acts as an anti-repressor by antagonizing YuxN repression.
messages: Inhibitor of Spx proteolytic control. Acts by interacting with SpxH/YjbH, which disrups interaction between SpxH and Spx, and inhibits SpxH-enhanced proteolysis of Spx by ClpXP (PubMed:21378193). Required for the stabilization of Spx and activation of Spx-regulated genes in response to cell wall stress (PubMed:30001325).
messages: Interacts with SpxH.

224308:BSU 37120
gene id: 224308:BSU_37120
uniprot id: A0A6M3ZGZ8
uniprot id: P13243
names: Cofactor
names: Function
names: PTM
names: Similarity
messages: Belongs to the class II fructose-bisphosphate aldolase family.
messages: Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.
messages: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.
messages: Phosphorylated during sporulation.

224308:BSU 30930
gene id: 224308:BSU_30930
uniprot id: A0A6M4JMH5
uniprot id: O34694
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TspO/BZRP family.
messages: Cell membrane
messages: Membrane

224308:BSU 37000
gene id: 224308:BSU_37000
uniprot id: A0A6M3ZGY3
uniprot id: P45873
names: Caution
names: Function
names: Similarity
messages: Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.

224308:BSU 01360
gene id: 224308:BSU_01360
uniprot id: A0A6M4JEQ4
uniprot id: P16336
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SecY/SEC61-alpha family.
messages: Cell membrane
messages: Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA (By similarity). Interacts with FloT (PubMed:23651456).
messages: Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Membrane raft
messages: The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.

224308:BSU 06490
gene id: 224308:BSU_06490
uniprot id: A0A6M4JFR7
uniprot id: P00497
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Allosterically regulated; subject to end product regulation by purine nucleotides.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Binds 1 [4Fe-4S] cluster per subunit. The [4Fe-4S] cluster requires a potential lower than -600 mV for reduction.
messages: Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
messages: Homotetramer.
messages: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

224308:BSU 19669
gene id: 224308:BSU_19669
uniprot id: C0H433
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 20050
gene id: 224308:BSU_20050
uniprot id: O34479
names: Function
messages: A possible homing endonuclease, it is entirely encoded within the YosP intron.

224308:BSU 14310
gene id: 224308:BSU_14310
uniprot id: A0A6M3ZFL0
uniprot id: O31706
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the MoaD family.
messages: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) by MoeB, then thiocarboxylated (-COSH) by IscS.
messages: Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Forms a stable heterotetrameric complex of 2 MoaD and 2 MoeB during adenylation of MoaD by MoeB. During catalysis MoaD shuttles between the two heterotetrameric complexes (By similarity).
messages: Involved in sulfur transfer in the conversion of molybdopterin precursor Z to molybdopterin.

224308:BSU 23040
gene id: 224308:BSU_23040
uniprot id: A0A6M4JLE2
uniprot id: P50727
names: Cofactor
names: Function
messages: Binds 1 [4Fe-4S] cluster.
messages: Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
messages: Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin may act as a phosphodonor to cytochrome P450 BioI.

224308:BSU 05020
gene id: 224308:BSU_05020
uniprot id: O31492
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Phr family.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably subject to only one processing event, at the N-terminal end of the mature peptide.
messages: Cytoplasm
messages: Encoded by the mobile genetic element ICEBs1.
messages: Expression is stimulated by conditions of low nutrient availability and high cell density.
messages: Intercellular signaling molecule that inhibits excision of the mobile genetic element ICEBs1 when cells are crowded by cells that contain ICEBs1 and produce the PhrI peptide (PubMed:16105942). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (By similarity). Acts by inhibiting RapI activity (PubMed:16105942).
messages: Secreted

224308:BSU 06720
gene id: 224308:BSU_06720
uniprot id: A0A6M3Z8A9
uniprot id: O31502
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the diacylglycerol/lipid kinase family.
messages: Binds 1 Mg(2+) ion per subunit. This ion appears to have a structural role and is required for catalytic activity.
messages: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway. Is more active toward long-chain DAG compared with short-chain DAG. Is not able to phosphorylate substrates other than DAG, such as monoacylglycerol, ceramide, undecaprenol, phosphatidylinositol, or sphingosine.
messages: Cells lacking this gene are not viable. They exhibit accumulation of diacylglycerol, disappearance of phosphatidylglycerol, and the cessation of lipoteichoic acid formation.

224308:BSU 38120
gene id: 224308:BSU_38120
uniprot id: A0A6M3ZHH9
uniprot id: P39604
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SEDS family. MrdB/RodA subfamily.
messages: Cell membrane
messages: Increased expression mediated by SigM is both necessary and sufficient to confer intrinsic resistance to moenomycin and viability to cells lacking aPBPs.
messages: Induced by the ECF sigma factor SigM.
messages: Membrane
messages: Peptidoglycan polymerase that is essential for cell wall elongation (PubMed:9622350, PubMed:27525505). Also required for the maintenance of the rod cell shape (PubMed:9622350).
messages: Peptidoglycan polymerase that is essential for cell wall elongation.

224308:BSU 12530
gene id: 224308:BSU_12530
uniprot id: P39782
names: Function
names: Similarity
messages: May function as a transcriptional antiterminator.
messages: To B.subtilis YqaM.

224308:BSU 31319
gene id: 224308:BSU_31319
uniprot id: C0H3Q4
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27830
gene id: 224308:BSU_27830
uniprot id: P94446
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: Present in spore but not in vegetative cell.
messages: Spore cortex
messages: Transcribed by SigG at time T3 of sporulation.

224308:BSU 20920
gene id: 224308:BSU_20920
uniprot id: O31933
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27770
gene id: 224308:BSU_27770
uniprot id: O05389
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.

224308:BSU 39220
gene id: 224308:BSU_39220
uniprot id: Q07836
names: Disruption Phenotype
names: Function
names: Induction
messages: A double wapA-wapI deletion strain is growth inhibited when cocultured with wild-type cells. When wapI is reintroduced it restores growth in a cognate toxin-dependent fashion (PubMed:23572593). Growth inhibition by wild-type cells is strongest on LB medium and less effective on media that promote biofilm formation (MSgg and 2xSGG) (PubMed:34280190).
messages: Constitutively and highly expressed on solid and in liquid medium, with or without biofilm formation by 12 hours of culture. Repressed by DegU.
messages: Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. Neutralizes the tRNase activity of cognate toxin WapA upon expression in E.coli. Does not inhibit WapA from other strains of B.subtilis. The WapA C-terminus cannot be expressed on its own in E.coli, however it can be cloned in the presence of its cognate immunity protein gene. Cell contact is necessary for growth inhibition (PubMed:23572593). Unlike the LXG toxin-immunity modules, WapAI mediates competition under shaking culture conditions (PubMed:34280190).

224308:BSU 12660
gene id: 224308:BSU_12660
uniprot id: P54332
names: Similarity
messages: To B.subtilis YqbM.

224308:BSU 10400
gene id: 224308:BSU_10400
uniprot id: P40397
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 33450
gene id: 224308:BSU_33450
uniprot id: O32215
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the helicase family. UvrD subfamily.
messages: Catalyzes the unwinding of duplex DNA in the 3' to 5' direction; this reaction is dependent on the hydrolysis of ATP.
messages: Cytoplasm
messages: Interacts with the RNA polymerase core.

224308:BSU 02380
gene id: 224308:BSU_02380
uniprot id: O31460
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression controlled by a sigma-Y-regulated promoter which needs the sigma-Y factor for the binding of the RNA polymerase and subsequent transcription.

224308:BSU 08730
gene id: 224308:BSU_08730
uniprot id: A0A6M4JE69
uniprot id: P71086
names: Cofactor
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Fur family.
messages: Binds 1 Mn(2+) or Fe(2+) ion per subunit.
messages: Binds 1 zinc ion per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: Hydrogen and organic peroxide sensor. Represses the expression of a regulon of peroxide-inducible genes such as katA, ahpC, ahpF, the heme biosynthesis operon (hemAXCDBL), fur, perR, zosA and mrgA.
messages: Possibly oxidized on a cysteine residue; the Cys-SOH formed in response to oxidative signaling may rapidly react with a Cys-SH to form a disulfide bond, leading to the loss of metal ion and inactivation of repressor function. Oxidized PerR can be further reduced by thiol reductants and repressor activity restored.

224308:BSU 07750
gene id: 224308:BSU_07750
uniprot id: O34708
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.
messages: Cell membrane

224308:BSU 25030
gene id: 224308:BSU_25030
uniprot id: P54486
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00950
gene id: 224308:BSU_00950
uniprot id: A0A6M3Z756
uniprot id: O31418
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MrnC RNase family.
messages: Cells accumulate precursors and mature forms of 23S rRNA with alternative 5' and 3' ends. Defects are more marked during exponential growth.
messages: Cytoplasm
messages: Homodimer.
messages: Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.
messages: Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors. Cleaves more efficiently on assembled 50S ribosomal subunits. Cleavage is strongly stimulated by ribosomal protein L3 (RplC); 20-30% DMSO can replace RplC, suggesting RplC may alter rRNA conformation.

224308:BSU 19570
gene id: 224308:BSU_19570
uniprot id: O34543
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the extradiol ring-cleavage dioxygenase family.
messages: Binds 1 Fe(2+) ion.
messages: Cytoplasm
messages: No visible phenotype. Growth is not inhibited by 2-methylhydroquinone or catechol.
messages: Putative ring-cleavage dioxygenase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 25780
gene id: 224308:BSU_25780
uniprot id: A0A6M3ZEC9
uniprot id: P45947
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activity is potassium and sulfate-independent.
messages: Belongs to the low molecular weight phosphotyrosine protein phosphatase family. Thioredoxin-coupled ArsC subfamily.
messages: Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)] (PubMed:16797027). In vitro, can dephosphorylate para-nitrophenyl phosphate (pNPP) (PubMed:11698660).
messages: Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)].
messages: Cytoplasm
messages: Induced by arsenate, arsenite, and antimonite.
messages: Monomer.
messages: Mutant is sensitive to arsenate but is still resistant to arsenite.

224308:BSU 41000
gene id: 224308:BSU_41000
uniprot id: A0A6M4JR87
uniprot id: P25813
names: Caution
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Low-level streptomycin resistance.
messages: Specifically methylates the N7 position of guanine in position 535 of 16S rRNA.

224308:BSU 12420
gene id: 224308:BSU_12420
uniprot id: O34703
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AAA ATPase family.
messages: Cytoplasm
messages: Expression is sigma W-dependent. Induced by alkali stress. Strongly induced at the end of the exponential growth phase.
messages: Shows ATPase activity.

224308:BSU 40090
gene id: 224308:BSU_40090
uniprot id: A0A6M4JQZ3
uniprot id: P80239
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
messages: By heat shock, salt stress, oxidative stress and glucose limitation.
messages: Cytoplasm
messages: Homodimer.
messages: Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.
messages: The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by AhpF.
messages: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.

224308:BSU 09550
gene id: 224308:BSU_09550
uniprot id: A0A6M4JLI3
uniprot id: O07585
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0053 family.
messages: Cell membrane
messages: Membrane

224308:BSU 28550
gene id: 224308:BSU_28550
uniprot id: P94548
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Homodimer. Binds to DNA.
messages: Transcriptional regulator in fatty acid degradation. Represses transcription of genes required for fatty acid transport and beta-oxidation, including acdA, fadA, fadB, fadE, fadF, fadG, fadH, fadM, fadN, lcfA and lcfB. Binding of FadR to DNA is specifically inhibited by long chain fatty acyl-CoA compounds of 14-20 carbon atoms in length.

224308:BSU 16450
gene id: 224308:BSU_16450
uniprot id: P40403
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the CheC family.
messages: Cells fail to synthesize flagellin protein and grow as long filaments. They do not respond to attractants. CheC mutants showed overmethylation of the MCPs compared with wild-type strain.
messages: Forms a complex with CheD.
messages: Involved in restoring normal CheY-P levels following the addition of attractant by increasing the rate of CheY-P hydrolysis. Is only 6% as active as FliY, which indicates that CheC may function after addition of an attractant to cope with increased levels of CheY-P whereas FliY may function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P. In addition, it was shown to prevent methylation of the methyl-accepting chemotaxis proteins (MCPs). Inhibits CheD.

224308:BSU 01420
gene id: 224308:BSU_01420
uniprot id: A0A6M4JC54
uniprot id: P04969
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS11 family.
messages: Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Interacts with proteins S7 and S18. Binds to IF-3 (By similarity). Interacts with VmlR (PubMed:30126986). Interacts with BrxC (PubMed:33722570).
messages: Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.

224308:BSU 07820
gene id: 224308:BSU_07820
uniprot id: P39796
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Cells lacking this gene are constitutive in the expression of the trePA operon.
messages: Dimer of dimers.
messages: Probably induced by trehalose-6-phosphate.
messages: Repressor for the trePA operon. It is able to bind trehalose-6-phosphate.

224308:BSU 21930
gene id: 224308:BSU_21930
uniprot id: A0A6M3ZDM5
uniprot id: P51777
names: Disruption Phenotype
names: Induction
names: Subcellular Location Annotation
messages: A double cshB-cspD mutant grows slowly at 15 degrees Celsius.
messages: Cytoplasm
messages: In response to low temperature.

224308:BSU 31620
gene id: 224308:BSU_31620
uniprot id: A0A6M4JMV3
uniprot id: O05260
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit C family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 28380
gene id: 224308:BSU_28380
uniprot id: P39072
names: Developmental Stage
names: Function
messages: Expressed shortly after the beginning of sporulation.
messages: Unknown. Affects both sporulation and germination.

224308:BSU 00330
gene id: 224308:BSU_00330
uniprot id: A0A6M4JEH4
uniprot id: P37542
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the YabA family.
messages: Interacts with both DnaA and DnaN, acting as a bridge between these two proteins.
messages: Involved in initiation control of chromosome replication.
messages: Sensitive to DNA damage.

224308:BSU 16290
gene id: 224308:BSU_16290
uniprot id: A0A6M4JK13
uniprot id: P23446
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum basal body
messages: Belongs to the flagella basal body rod proteins family.
messages: The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each (By similarity).

224308:BSU 35360
gene id: 224308:BSU_35360
uniprot id: A0A6M4JPM4
uniprot id: P02968
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum
messages: Belongs to the bacterial flagellin family.
messages: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
messages: Flagellin is the subunit which polymerizes to form the filaments of bacterial flagella. Assembly into flagella requires FliW (PubMed:16936039). Acts as a homeostatic autoinhibitory regulator to control its own cytoplasmic levels. Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis. Flagellin binds to assembly factor FliW, freeing translation regulator CsrA to repress translation of the flagellin mRNA. When the flagellar hook is assembled flagellin is secreted, depleting intracellular flagellin, which frees FliW to interact with CsrA. This derepresses flagellin translation and provides protein for flagellar assembly. Once the flagellar filament is completed cytoplasmic flagellin levels rise and CsrA translation repression of flagellin reinitiates (PubMed:21895793).
messages: Interacts with FliW in a 1:1 complex (PubMed:16936039, PubMed:21895793). Forms a 3-way complex of Hag, FliS and FliW, in which Flis and FliW do not directly interact (PubMed:23144244).
messages: Secreted
messages: Under positive control of SigD (PubMed:11987133). Repressed by CsrA; repression is greater in the 1A96 than 168 genetic background and higher in minimal than rich medium (PubMed:17555441).
messages: cell wall

224308:BSU 01030
gene id: 224308:BSU_01030
uniprot id: A0A6M4JE41
uniprot id: Q06797
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL1 family.
messages: Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Interacts with RNA helicase CshA (PubMed:23175651). Interacts with VmlR (PubMed:30126986).
messages: Part of the 50S ribosomal subunit.
messages: Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.

224308:BSU 14580
gene id: 224308:BSU_14580
uniprot id: A0A6M4JJM2
uniprot id: P21881
names: Function
names: Subunit
messages: Heterodimer of an alpha and a beta chain.
messages: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity.
messages: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 12650
gene id: 224308:BSU_12650
uniprot id: A0A6M4JFL3
uniprot id: P54331
names: Similarity
messages: Belongs to the myoviridae tail sheath protein family.

224308:BSU 03160
gene id: 224308:BSU_03160
uniprot id: O31474
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 24110
gene id: 224308:BSU_24110
uniprot id: O32015
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 06530
gene id: 224308:BSU_06530
uniprot id: A0A6M3Z844
uniprot id: P12039
names: Cofactor
names: Similarity
messages: Belongs to the GARS family.
messages: Binds 1 Mg(2+) or Mn(2+) ion per subunit.

224308:BSU 37510
gene id: 224308:BSU_37510
uniprot id: P70997
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: In the C-terminal section; belongs to the transpeptidase family.
messages: In the N-terminal section; belongs to the glycosyltransferase 51 family.
messages: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane.
messages: No visible phenotype; due to the redundancy with PbpF. Spores have normal heat-resistance, cortex structure, and germination and outgrowth properties.
messages: Sporulation specific. Expressed predominantly, if not exclusively, in the forespore.

224308:BSU 12710
gene id: 224308:BSU_12710
uniprot id: P54337
names: Similarity
messages: To B.subtilis YqbR.

224308:BSU 17950
gene id: 224308:BSU_17950
uniprot id: P45710
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 34700
gene id: 224308:BSU_34700
uniprot id: A0A6M3ZHA9
uniprot id: O06980
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 00670
gene id: 224308:BSU_00670
uniprot id: A0A6M4JCA5
uniprot id: P37563
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the tRNA(Ile)-lysidine synthase family.
messages: Cytoplasm
messages: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
messages: The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

224308:BSU 34130
gene id: 224308:BSU_34130
uniprot id: O07012
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the glycosyl hydrolase 42 family.
messages: Homotrimer.
messages: Inhibited by zinc, cobalt and copper ions.
messages: Involved in galactan degradation (PubMed:27501980). Hydrolyzes galactooligosaccharides released by the endo-beta-1,4-galactanase GanB from galactan (PubMed:17056685, PubMed:27501980). Degrades galactotetraose, galactotriose and galactobiose, generating galactose as the end product (PubMed:27501980). It is unable to use lactose (PubMed:17056685). In vitro, shows maximal activity with o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-galactopyranoside (PNPG) as substrates, trace activity with p-nitrophenyl-alpha-L-arabinopyranoside and o-nitrophenyl-beta-D-fucopyranoside as substrates, but no activity with p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside or p-nitrophenyl-alpha-L-arabinofuranoside as substrates (PubMed:17056685).
messages: No chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) hydrolyzation. Reduces beta-galactosidase activity observed with polygalacturonic acid, citrus and apple pectins, galactan and soy flour.
messages: Repressed by the transcriptional regulator GanR and induced by galactobiose. Also repressed by glucose.

224308:BSU 30630
gene id: 224308:BSU_30630
uniprot id: A0A6M4JLD3
uniprot id: O35013
names: Activity Regulation
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: According to PubMed:14761999, can complement an E.coli mutT mutant. According to PubMed:15576788, cannot complement an E.coli mutT mutant.
messages: Belongs to the Nudix hydrolase family.
messages: Expressed during both vegetative growth and early stage of sporulation.
messages: Growing cells lacking this gene have a 4-fold increased spontaneous mutation frequency (a mild mutator phenotype), as well as an increased sensitivity to DNA-damaging agents such as H(2)O(2) or t-BHP. These phenotypes are increased in a double ytkD/mutT disruption (PubMed:16513759). They are also seen in stationary phase cells. Triple ytkD/mutM/mutY disrupted strains show increased mutation rates during exponential and stationary phase (PubMed:19011023).
messages: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions (By similarity). Functions, in conjunction with MutT, to protect vegetatively growing cells from DNA-damaging agents such as H(2)O(2) or t-BHP (t-butylhydroperoxide). The 2 proteins do not however protect spores. According to PubMed:15576788, phosphohydrolase that catalyzes the hydrolysis of all common nucleoside triphosphates as well as of the mutagenic analog 8-oxo-dGTP. The high catalytic efficiency on dGTP is in contrast to results from PubMed:14761999. According to PubMed:14761999, catalyzes the hydrolysis of 8-oxo-dGTP with a specific activity 413 times higher than that exhibited against dGTP. Preferentially catalyzes the hydrolysis of 8-oxo-dGTP and 8-oxo-GTP. According to PubMed:15576788, hydrolyzes nucleoside triphosphates in a stepwise fashion through the diphosphate to the monophosphate, releasing two molecules of inorganic orthophosphate.
messages: Not induced by oxidative damage (following treatment with paraquat or hydrogen peroxide). Not induced by mitomycin C. Not induced by sigma-B general stress inducers such as sodium chloride, ethanol or heat.

224308:BSU 03210
gene id: 224308:BSU_03210
uniprot id: A0A6M4JFA9
uniprot id: P94391
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family. RocA subfamily.
messages: Deletion of the putBCP operon abolishes L-proline utilization.
messages: Important for the use of proline as a sole carbon and energy source or a sole nitrogen source.
messages: The expression of the putBCP operon is induced in a PutR-dependent fashion by very low concentrations of L-proline in the growth medium. CodY represses the operon by displacing PutR from DNA.

224308:BSU 04970
gene id: 224308:BSU_04970
uniprot id: A0A6M3Z8B5
uniprot id: P96645
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Deletion mutant shows no detectable conjugative transfer of ICEBs1.
messages: Exhibits both muramidase and DL-endopeptidase activities (PubMed:18305117, PubMed:24532767). The N-terminal region acts as a N-acetylmuramidase, which cleaves the bond between N-acetylmuramic acid and N-acetyl-D-glucosamine (MurNAc-GlcNAc) in peptidoglycan (PubMed:18305117). The C-terminal region acts as a DL-endopeptidase that cleaves the bond between D-gamma-glutamate and meso-diaminopimelic acid (PubMed:18305117). Cannot degrade purified B.anthracis peptidoglycan, which differ from those of B.subtilis (PubMed:24532767). CwlT is required for ICEBs1 conjugation: the muramidase activity is essential, whereas the peptidase activity is partially dispensable for transfer of ICEBs1 (PubMed:24532767).
messages: Exogenous CwlT causes lysis of B.subtilis and B.anthracis, most likely by causing at least minimal degradation of the cell wall.
messages: Secreted
messages: The cwlT gene is located in the region of the mobile genetic element ICEBs1, an integrative and conjugative element (ICE) found in B.subtilis.

224308:BSU 35550
gene id: 224308:BSU_35550
uniprot id: O32268
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 22150
gene id: 224308:BSU_22150
uniprot id: P50831
names: Similarity
messages: Belongs to the helicase family. DinG subfamily.

224308:BSU 17760
gene id: 224308:BSU_17760
uniprot id: A0A6M3ZBH4
uniprot id: O31809
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
messages: Cell membrane
messages: Involved in the germinative response to L-alanine. Could be an amino acid transporter (By similarity).
messages: Membrane

224308:BSU 31020
gene id: 224308:BSU_31020
uniprot id: O32077
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Alters assembly of FloT membrane rafts; more diffuse rafts are seen. Double floA-nfeD2 deletion mutants are wild-type (PubMed:22753055). No visible effect on FloT membrane assemblies (PubMed:27362352).
messages: Belongs to the NfeD family.
messages: Cell membrane
messages: Expression is sigma W-dependent. Up-regulated by alkali shock and by infection with phage SPP1.
messages: Membrane raft
messages: Plays a role in assembly of FloT membrane rafts, probably recruited to rafts by FloT.

224308:BSU 02740
gene id: 224308:BSU_02740
uniprot id: P70955
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system NatK/NatR that positively regulates the expression of the natAB operon. Acts by binding directly to the promoter of natAB.
messages: Phosphorylated by NatK.

224308:BSU 13530
gene id: 224308:BSU_13530
uniprot id: O31661
names: Function
names: Induction
messages: Expressed during growth and early stationary phase.
messages: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F.

224308:BSU 23790
gene id: 224308:BSU_23790
uniprot id: P54553
names: Cofactor
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 32380
gene id: 224308:BSU_32380
uniprot id: A0A6M3ZGB5
uniprot id: O32134
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.
messages: Cell membrane
messages: Membrane

224308:BSU 11840
gene id: 224308:BSU_11840
uniprot id: O31628
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 13310
gene id: 224308:BSU_13310
uniprot id: Q45666
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homodimer (PubMed:10864496, PubMed:25691471). Under conditions of nitrogen excess, TnrA forms a stable complex with feedback-inhibited GlnA. Interacts with GlnK-AmtB complex.
messages: The amino acid sequences of the N-terminal DNA binding domains of TnrA and GlnR are highly similar, and both proteins bind to DNA sequences with a common consensus sequence. In contrast, the C-terminal signal transduction domains of TnrA and GlnR have no homology.
messages: TnrA mutant is impaired in its ability to utilize allantoin, gamma-aminobutyrate, isoleucine, nitrate, urea and valine as nitrogen sources. During nitrogen-limited growth, transcription of the nrgAB, nasB, gabP, and ure genes is significantly reduced in the tnrA mutant. In contrast, the level of glnRA expression is 4-fold higher in the tnrA mutant than in wild-type cells during nitrogen restriction.
messages: Transcription regulator that actives the transcription of genes required for nitrogen assimilation such as nrgAB (ammonium transport), nasABCDEF (nitrate/nitrite assimilation), ureABC (urea degradation) and gabP (GABA transport), during nitrogen limitation (PubMed:8799114, PubMed:9603886, PubMed:10231480, PubMed:10864496, PubMed:11719184, PubMed:12823818). Also represses glnRA and gltAB in the absence of ammonium (PubMed:8799114, PubMed:9603886, PubMed:10231480, PubMed:10864496, PubMed:11719184, PubMed:12823818). On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, TnrA requires a DNA sequence of 17 nucleotides as minimal binding site (PubMed:10231480, PubMed:25691471).
messages: Under conditions of nitrogen excess, repressed by GlnR. Under conditions of nitrogen-limited growth, it positively regulates its own expression.
messages: Under conditions of nitrogen excess, the DNA-binding activity is inhibited by the formation of a stable complex with feedback-inhibited GlnA (PubMed:11719184, PubMed:25691471). The presence of glutamine and AMP increases the inhibitory activity of glutamine synthetase by more than 1000-fold (PubMed:11719184).

224308:BSU 05440
gene id: 224308:BSU_05440
uniprot id: P96688
names: Similarity
names: Subunit
messages: Belongs to the AB hydrolase superfamily.
messages: Monomer.

224308:BSU 14170
gene id: 224308:BSU_14170
uniprot id: A0A6M3ZA70
uniprot id: O34589
names: Function
names: Similarity
messages: Belongs to the flavodoxin family.
messages: Low-potential electron donor to a number of redox enzymes.

224308:BSU 35030
gene id: 224308:BSU_35030
uniprot id: O34817
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Subunit
messages: Binding to DNA is allosterically inhibited by an effector molecule (PubMed:20047956, PubMed:21602348, PubMed:24673833, PubMed:25564531). Binding of the effector to the C-terminal domain leads to a conformational change that modulates binding to DNA and thereby regulates transcription of the target genes (PubMed:20047956, PubMed:25564531). Glucosamine-6-phosphate (GlcN6P) and/or N-acetylglucosamine-6-phosphate (GlcNAc6P) are putative effectors of NagR (PubMed:20047956, PubMed:21602348, PubMed:24673833, PubMed:25564531). Binding of GlcNAc6P may prevent the protein-protein interactions responsible for polymerization along the DNA, but not the specific DNA binding (PubMed:24673833).
messages: Deletion of the gene results in the modulation of the expression of many important genes, probably indirectly due to an excess of the crucial molecules acetate, ammonia, and fructose-6-phosphate, resulting from complete hydrolysis of GlcNAc (PubMed:21602348). Disruption of the gene induces a slight but consistent increase in MapB activity (PubMed:16207374).
messages: Homodimer (PubMed:19342794, PubMed:20047956, PubMed:25564531). Forms dimers via the C-terminal effector-binding domain (PubMed:20047956). At high concentrations, probably forms polymers along the DNA (PubMed:24673833).
messages: Main transcriptional repressor of genes involved in N-acetylglucosamine (GlcNAc) transport and utilization (PubMed:21602348, PubMed:20047956, PubMed:24673833). Represses the expression of the nagAB and nagP operons by binding directly within their upstream regions (PubMed:21602348, PubMed:24673833). Binds to the DNA consensus sequence 5'-ATTGGTATAGACAACT-3' (PubMed:21602348). Also acts as a weak repressor of mapB expression (PubMed:16207374).
messages: The N-terminal region contains a canonical wHTH DNA-binding domain. It is linked via a 21 residue-long linker to the C-terminal domain, which binds the effector molecule.

224308:BSU 34860
gene id: 224308:BSU_34860
uniprot id: A0A6M4JPI5
uniprot id: O34912
names: Similarity
names: Subcellular Location Annotation
messages: Cytoplasm
messages: In the C-terminal section; belongs to the PRA-PH family.
messages: In the N-terminal section; belongs to the PRA-CH family.

224308:BSU 07590
gene id: 224308:BSU_07590
uniprot id: A0A6M4JDZ5
uniprot id: O34534
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Phosphorylated CitT binds to the citM promoter to activate the transcription of the citM-yflN operon.
messages: Phosphorylated by CitS.

224308:BSU 02410
gene id: 224308:BSU_02410
uniprot id: A0A6M3Z794
uniprot id: O31463
names: Cofactor
names: Disruption Phenotype
messages: Binds 1 zinc ion per subunit.
messages: Cells lacking this gene show reduced bacilysin biosynthetic activity.

224308:BSU 15620
gene id: 224308:BSU_15620
uniprot id: O34632
names: Function
names: Induction
names: Similarity
messages: Belongs to the CbiX family. SirB subfamily.
messages: Chelates iron to the siroheme precursor.
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 34870
gene id: 224308:BSU_34870
uniprot id: A0A6M3ZJL1
uniprot id: O34727
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the HisA/HisF family.
messages: Cytoplasm
messages: Heterodimer of HisH and HisF.
messages: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit (By similarity).
messages: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.

224308:BSU 32600
gene id: 224308:BSU_32600
uniprot id: O32156
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Probably part of the binding-protein-dependent transport system YurMNO.

224308:BSU 23730
gene id: 224308:BSU_23730
uniprot id: A0A6M3ZDX8
uniprot id: P54559
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 37150
gene id: 224308:BSU_37150
uniprot id: A0A6M3ZH10
uniprot id: P13242
names: Activity Regulation
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.
messages: Belongs to the CTP synthase family.
messages: CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.
messages: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.
messages: Homotetramer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 07840
gene id: 224308:BSU_07840
uniprot id: A0A6M4JG22
uniprot id: O34313
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 5'-nucleotidase family.
messages: Catalyzes the release of inorganic phosphate from 2',3'-cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'-nucleotidase activity. Does not catalyze the release of inorganic phosphate from 3',5'-cyclic nucleotides. Probably plays a role in the cellular reprocessing of nucleotides present in the medium, under conditions of phosphate shortage.
messages: Expression is induced in response to phosphate starvation in a PhoR-dependent manner.
messages: The N-terminal region (amino acids 35-623) is able to catalyze the release of phosphate from 2',3'-cyclic nucleotides, but not from 5'-nucleotides.
messages: cell wall

224308:BSU 12460
gene id: 224308:BSU_12460
uniprot id: O34391
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
messages: Secreted

224308:BSU 28770
gene id: 224308:BSU_28770
uniprot id: P94526
names: Function
names: Induction
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily.
messages: Catalyzes the dephosphorylation of C5 and C6 carbon sugars in vitro (PubMed:21575135, PubMed:25848029). Catalyzes the dephosphorylation of 3'-AMP and phosphoserine in vitro (PubMed:25848029).
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 36100
gene id: 224308:BSU_36100
uniprot id: A0A6M3ZGN0
uniprot id: O05218
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the gamma-glutamyltransferase family.
messages: Cleaved by autocatalysis into a large and a small subunit.
messages: Grows normally with glutathione as a sulfur source.
messages: Is not involved in the utilization of extracellular glutathione as a sulfur source.
messages: Overexpressed protein with an N-terminal His tag has been reported not to hydrolyze glutathione; it is not clear if the construct is processed to 2 subunits (PubMed:14762019).
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: This enzyme consists of two polypeptide chains, which are synthesized from a single polypeptide.
messages: This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.

224308:BSU 24380
gene id: 224308:BSU_24380
uniprot id: P49783
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27460
gene id: 224308:BSU_27460
uniprot id: A0A6M4JNU1
uniprot id: O34606
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex GlnHMPQ involved in glutamine transport. Probably responsible for the translocation of the substrate across the membrane (By similarity).
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: The complex is composed of two ATP-binding proteins (GlnQ), two transmembrane proteins (GlnM and GlnP) and a solute-binding protein (GlnH).

224308:BSU 34560
gene id: 224308:BSU_34560
uniprot id: A0A6M4JPF4
uniprot id: O06994
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 13 family.
messages: Cytoplasm
messages: Hydrolyzes various disaccharides such as sucrose, maltose, and isomaltose with different efficiencies. Also hydrolyzes longer maltodextrins from maltotriose up to maltohexaose, but not maltoheptaose, palatinose, isomaltotriose, or isomaltotetraose.

224308:BSU 15180
gene id: 224308:BSU_15180
uniprot id: A0A6M4JGG3
uniprot id: Q03523
names: Caution
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MurCDEF family. MurE subfamily.
messages: Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.
messages: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 35790
gene id: 224308:BSU_35790
uniprot id: A0A6M3ZH45
uniprot id: P39841
names: Cofactor
names: Similarity
messages: Belongs to the mannose-6-phosphate isomerase type 1 family.
messages: Binds 1 zinc ion per subunit.

224308:BSU 01060
gene id: 224308:BSU_01060
uniprot id: P37872
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 17410
gene id: 224308:BSU_17410
uniprot id: Q06320
names: Activity Regulation
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze type A cell walls such as B.subtilis. Its main function is to lyze the mother cell wall peptidoglycan, playing a role during sporulation.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
messages: Contains an N-terminal catalytic domain and two C-terminal tandem repeat sequences that play an important role in peptidoglycan binding.
messages: Induced at 6 to 7 hours after sporulation.
messages: Inhibited by EDTA.
messages: cell wall

224308:BSU 31120
gene id: 224308:BSU_31120
uniprot id: O32083
names: Activity Regulation
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 73 family.
messages: Expressed during vegetative growth.
messages: Expressed under control of sigma-A RNA polymerase.
messages: Inhibited by EDTA.
messages: Is the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Catalyzes the hydrolysis of 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid residues in peptidoglycan. Acts processively from the ends of the glycan strands. Also plays a role in motility, chemotaxis and cell division.
messages: cell wall

224308:BSU 40820
gene id: 224308:BSU_40820
uniprot id: P37512
names: Similarity
messages: To C.elegans B0495.5.

224308:BSU 05200
gene id: 224308:BSU_05200
uniprot id: P96665
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25310
gene id: 224308:BSU_25310
uniprot id: A0A6M4JKV7
uniprot id: P19638
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial diacylglycerol kinase family.
messages: Catalyzes the phosphorylation of undecaprenol in vitro, which is probably the physiological substrate. Exhibits no detectable activity against other substrates such as monoacylglycerol, ceramide, or diacylglycerol (DAG). Appears indispensable for the maintenance of spore stability and viability in B.subtilis.
messages: Cell membrane
messages: Cells lacking this gene exhibit an abnormal cortex structure in mutant endospores 6 hours after the onset of sporulation, an indication of cortex degeneration. In addition, they display a significant decrease in the dipicolinic acid content of mutant spores.
messages: Mainly expressed during the vegetative phase of growth.
messages: Membrane
messages: Mn(2+), Zn(2+), Cd(2+) and Co(2+) support activity to lesser extents.
messages: Was originally thought to be a diacylglycerol kinase.

224308:BSU 29060
gene id: 224308:BSU_29060
uniprot id: A0A6M4JNX1
uniprot id: O34932
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CoaE family.
messages: Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.
messages: Cytoplasm

224308:BSU 35910
gene id: 224308:BSU_35910
uniprot id: P36944
names: Function
messages: Transcriptional repressor for the ribose rbsDACBK operon.

224308:BSU 32680
gene id: 224308:BSU_32680
uniprot id: O32163
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NifU family.
messages: Bind 1 Zn(2+) per monomer.
messages: Essential, it cannot be deleted. Upon depletion cells grow very slowly while aconitase and succinate dehydrogenase, both of which contain Fe-S clusters, have decreased activity. Upon depletion no change in reactive oxygen species is observed, while a modified bacillibactin (BB), an endogenous siderophore, is produced.
messages: Interacts with SufS; this interaction enhances SufS cysteine desulfurase activity. Interacts with frataxin/Fra (PubMed:27382962).
messages: Part of the SUF-like system that mediates the biosynthesis of iron-sulfur (Fe-S) clusters. Acts as a sulfurtransferase and thus transfers sulfur from SufS to SufB (PubMed:29292548). Mechanistically, the transfer from SufS to SufU is triggered by zinc-ligand swapping that provides a free thiol from SufU to accept sulfur from SufS (PubMed:29235855).
messages: The inhibition of SufS activity by the Cys-41 mutation in this protein has been described as competitive and non-competitive inhibition (PubMed:21236255, PubMed:24321018).
messages: Was originally thought to be an iron-sulfur cluster assembly scaffold protein (PubMed:20097860, PubMed:21236255). It is now thought to be a zinc-dependent sulfur transfer protein (PubMed:24321018).

224308:BSU 17970
gene id: 224308:BSU_17970
uniprot id: Q45058
names: Function
names: Induction
messages: Induced during development under sigma-K control and negatively regulated by GerE.
messages: Involved in spore outer coat assembly. May be part of a cross-linked insoluble skeleton that surrounds the spore, serves as a matrix for the assembly of additional outer coat material, and confers structural stability to the final structure.

224308:BSU 32370
gene id: 224308:BSU_32370
uniprot id: A0A6M3ZJ61
uniprot id: O32133
names: Cofactor
names: Similarity
messages: Belongs to the 5'-nucleotidase family.
messages: Belongs to the metallophosphoesterase superfamily.
messages: Binds 2 divalent metal cations.

224308:BSU 18460
gene id: 224308:BSU_18460
uniprot id: A0A6M3ZEV4
uniprot id: P20668
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Activated by alpha-ketoglutarate and inhibited by glutamate and by RocG.
messages: Belongs to the LysR transcriptional regulatory family.
messages: Induced by glucose, repressed by arginine, less protein accumulates when grown in the presence of glucose and arginine (at protein level).
messages: Interacts with gutamate dehydrogenase RocG.
messages: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression.

224308:BSU 09120
gene id: 224308:BSU_09120
uniprot id: A0A6M4JLE6
uniprot id: P54595
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 33210
gene id: 224308:BSU_33210
uniprot id: O34989
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YvrG/YvrH that positively regulates 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sunT-bdbA-yolJ-bdbB, sigO-rsoA, and sigX-rsiX), and negatively regulates the lytABC operon. Probably activates YvrH by phosphorylation.

224308:BSU 40920
gene id: 224308:BSU_40920
uniprot id: A0A6M3ZKN5
uniprot id: P37518
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.
messages: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.
messages: No visible phenotype.
messages: Weakly expressed during exponential growth in rich medium, stops at onset of stationary phase. In minimal medium is more strongly expressed and continues into stationary phase, part of the ychF-rpsF-ssbA-rpsR operon (PubMed:14762004).

224308:BSU 08620
gene id: 224308:BSU_08620
uniprot id: O31583
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Autoregulated. Transcriptionally regulated by sigma-K. Expressed during exponential growth.
messages: Cell membrane
messages: Cells lacking this gene display increased rates of transcription of mutY, fabL, sspE and yfhP.
messages: May act as a negative regulator for the transcription of mutY, fabL, sspE and yfhP.

224308:BSU 31440
gene id: 224308:BSU_31440
uniprot id: Q08432
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily.
messages: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine.
messages: Constitutively expressed at a low level.
messages: No visible phenotype.

224308:BSU 06200
gene id: 224308:BSU_06200
uniprot id: O35004
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0603 family.
messages: Cell membrane

224308:BSU 07200
gene id: 224308:BSU_07200
uniprot id: A0A6M4JFX2
uniprot id: O31539
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the BI1 family.
messages: Cell membrane
messages: Membrane

224308:BSU 38530
gene id: 224308:BSU_38530
uniprot id: A0A6M3ZHH5
uniprot id: P39578
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DltD family.
messages: Cell membrane
messages: Involved in the D-alanylation of lipoteichoic acid (LTA). Could be responsible for the transfer of DltC-carried D-alanyl groups to cell membrane phosphatidylglycerol (PG), or alternatively of D-alanine residues from D-Ala-undecaprenol phosphate to the poly(glycerophosphate) chains of LTA. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.

224308:BSU 12100
gene id: 224308:BSU_12100
uniprot id: O34798
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Activated by divalent metal cations; Mn(2+) is the most efficient, followed by Ca(2+) and Mg(2+). In contrast to PgdA from S.pneumoniae, these ions are not absolutely required for deacetylase activity.
messages: Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in peptidoglycan, a modification that confers resistance to lysosyme. Is not able to deacetylate N-acetylglucosamine (GlcNAc) residues in peptidoglycan, but can deacylate chitin oligomers such as GlcNAc4 and GlcNAc5. Is essentially not active toward chitosan (partially deacetylated GlcNAc polymer) and has very low activity toward chitin (GlcNAc polymer). Does not deacetylate GlcNAc.
messages: Cell membrane
messages: Cells lacking this gene show sensitivity to lysosyme, in contrast to wild-type.
messages: Derepression of pdaC in cells depleted for yycFG leads to increased resistance of the cell walls to lysozyme digestion.
messages: In the C-terminal section; belongs to the polysaccharide deacetylase family.
messages: In the N-terminal section; belongs to the RsiV family.
messages: Negatively regulated by the two-component system YycFG.
messages: The product of deacetylated GlcNAc4 by PdaC is GlcNAc-GlcNAc-GlcN-GlcNAc.
messages: Was originally described as a deoxyribonuclease (PubMed:17878218), but it was later shown that PdaC has no DNase activity at all (PubMed:22277649).

224308:BSU 38390
gene id: 224308:BSU_38390
uniprot id: A0A6M3ZLM0
uniprot id: P39584
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane.

224308:BSU 09300
gene id: 224308:BSU_09300
uniprot id: A0A6M3ZE51
uniprot id: P18158
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
messages: Cytoplasm
messages: Requires glycerol 3-phosphate and the GlpP product; repressed by glucose.

224308:BSU 04020
gene id: 224308:BSU_04020
uniprot id: O31482
names: Similarity
messages: Belongs to the dienelactone hydrolase family.

224308:BSU 32520
gene id: 224308:BSU_32520
uniprot id: A0A6M4JLB6
uniprot id: O32148
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
messages: Catalyzes the transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine. Glyoxylate is the preferred substrate, but other amino-group acceptors can be used.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin is added during limiting-nitrogen conditions.
messages: Homodimer.

224308:BSU 31800
gene id: 224308:BSU_31800
uniprot id: A0A6M4JN22
uniprot id: O32095
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.
messages: Cell membrane
messages: Membrane

224308:BSU 09810
gene id: 224308:BSU_09810
uniprot id: O07541
names: Induction
messages: By mitomycin C and UV irradiation which requires RecA.

224308:BSU 33660
gene id: 224308:BSU_33660
uniprot id: O32236
names: Disruption Phenotype
names: Function
messages: Cells lacking this gene display a derepression of rapG and rapH expression, which leads to a down-regulation of sfrA that in turn leads to the expression abolition of both comK and the late com operon comG.
messages: Represses the expression of yvaM and both rapG and rapH. Binds directly to the promoter regions of yvaM, rapG and rapH.

224308:BSU 39380
gene id: 224308:BSU_39380
uniprot id: A0A6M4JQT1
uniprot id: P42068
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the arginase family.
messages: Binds 2 manganese ions per subunit.
messages: Binds 2 manganese ions per subunit. Can also use other divalent cation, with lower efficiency (PubMed:4990470).
messages: Catalyzes the conversion of N-formimidoyl-L-glutamate to L-glutamate and formamide.

224308:BSU 37800
gene id: 224308:BSU_37800
uniprot id: P39632
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the BslA/BslB family.
messages: Has a minor role in biofilm architecture. May contribute to the surface hydrophobicity.
messages: Monomer in vitro.
messages: Secreted

224308:BSU 24360
gene id: 224308:BSU_24360
uniprot id: P49785
names: Developmental Stage
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Interacts with SpoIIQ.
messages: Involved in forespore engulfment. Forms a channel with SpoIIIAH that is open on the forespore end and closed (or gated) on the mother cell end. This allows sigma-E-directed gene expression in the mother-cell compartment of the sporangium to trigger the activation of sigma-G forespore-specific gene expression by a pathway of intercellular signaling.
messages: Specifically expressed in the mother cell during sporulation under the control of the sigma-E factor.

224308:BSU 10880
gene id: 224308:BSU_10880
uniprot id: A0A6M3Z988
uniprot id: Q796Q6
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 18550
gene id: 224308:BSU_18550
uniprot id: O34861
names: Induction
names: Similarity
messages: Belongs to the FGGY kinase family.
messages: Induced by sulfate, part of the yoaDCB operon.

224308:BSU 36080
gene id: 224308:BSU_36080
uniprot id: O05220
names: Similarity
messages: Belongs to the flavoredoxin family.

224308:BSU 09780
gene id: 224308:BSU_09780
uniprot id: O07544
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YheC/YheD family.
messages: Forespore outer membrane
messages: Involved in sporulation.
messages: Spore coat

224308:BSU 09940
gene id: 224308:BSU_09940
uniprot id: O07520
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression controlled by a sigma-E-regulated promoter which needs the sigma-E factor for the binding of the RNA polymerase and subsequent transcription.
messages: Required for efficient sporulation.

224308:BSU 02480
gene id: 224308:BSU_02480
uniprot id: A0A6M3Z709
uniprot id: P42237
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Phthalate permease family.
messages: Cell membrane
messages: Induced in the presence of D-glucarate or D-galactarate.
messages: Membrane
messages: Probably involved in the uptake of galactarate and/or D-glucarate.

224308:BSU 07350
gene id: 224308:BSU_07350
uniprot id: A0A6M4JJF3
uniprot id: O06478
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: A benzaldehyde dehydrogenase able to act on substrates with 3- and 4-hydroxy and methoxy substitutions; converts vanillin (4-hydroxy-3-methoxybenzaldehyde) to vanillic acid in vitro (PubMed:26658822). The physiological substrate is unknown (PubMed:26658822).
messages: Belongs to the aldehyde dehydrogenase family.
messages: Loss of conversion of vanillin to vanillic acid (PubMed:26658822).
messages: Transcriptionally regulated by SigD; part of the yfmT/yfmS operon.

224308:BSU 28470
gene id: 224308:BSU_28470
uniprot id: A0A6M3ZEX7
uniprot id: P08495
names: Activity Regulation
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aspartokinase family.
messages: Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.
messages: Lysine-sensitive. Regulated by degradation in response to starvation of cells for various nutrients. Ammonium starvation induced the fastest aspartokinase II decline, followed by amino acid starvation and glucose limitation.
messages: Tetramer consisting of 2 isoforms Alpha (catalytic and regulation) and of a homodimer of 2 isoforms Beta (regulation).

224308:BSU 17030
gene id: 224308:BSU_17030
uniprot id: P14016
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CotE family.
messages: Expressed during sporulation.
messages: Homooligomer.
messages: Morphogenic protein required for the assembly of the outer coat of the endospore. Is also a regulatory protein for the expression of cotA, cotB, cotC, cotH and other genes encoding spore outer coat proteins.
messages: Spore coat

224308:BSU 34170
gene id: 224308:BSU_34170
uniprot id: O07008
names: Function
messages: Negatively regulates the expression of the ganSPQAB operon (PubMed:2104611, PubMed:9287030, PubMed:27501980). Inhibits transcription of the operon by binding to an operator in the promoter region (PubMed:27501980). In the presence of galactobiose, GanR dissociates from the promoter, resulting in the expression of the gan operon (PubMed:27501980).

224308:BSU 14080
gene id: 224308:BSU_14080
uniprot id: O31696
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 14370
gene id: 224308:BSU_14370
uniprot id: A0A6M3ZA85
uniprot id: O31712
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Expressed in exponential-phase cells.
messages: Membrane
messages: Membrane raft
messages: Part of a complex composed of YknX, YknY and YknZ. The complex interacts with YknW.
messages: Part of an unusual four-component transporter, which is required for protection against the killing factor SdpC (sporulation-delaying protein).

224308:BSU 06690
gene id: 224308:BSU_06690
uniprot id: A0A6M4JH35
uniprot id: O30509
names: Function
names: Similarity
names: Subunit
messages: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
messages: Belongs to the GatB/GatE family. GatB subfamily.
messages: Heterotrimer of A, B and C subunits.

224308:BSU 27050
gene id: 224308:BSU_27050
uniprot id: A0A6M4JK33
uniprot id: P26381
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in fructose transport.

224308:BSU 04290
gene id: 224308:BSU_04290
uniprot id: O31487
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37860
gene id: 224308:BSU_37860
uniprot id: P39626
names: Similarity
messages: Belongs to the CMP-NeuNAc synthase family.

224308:BSU 30740
gene id: 224308:BSU_30740
uniprot id: A0A6M3ZFC5
uniprot id: O34500
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-3 integral membrane protein family.
messages: Cell membrane
messages: Membrane
messages: Probably part of the ABC transporter complex MntABCD involved in manganese import. Probably responsible for the translocation of the substrate across the membrane.
messages: Repressed by MntR in the presence of manganese.
messages: The complex is probably composed of two ATP-binding proteins (MntB), two transmembrane proteins (MntC and MntD) and a solute-binding protein (MntA).

224308:BSU 13350
gene id: 224308:BSU_13350
uniprot id: A0A6M3ZAB7
uniprot id: C0SPB9
names: Similarity
messages: Belongs to the glycosyltransferase 28 family.

224308:BSU 12370
gene id: 224308:BSU_12370
uniprot id: Q9JMQ1
names: Disruption Phenotype
names: Function
names: Induction
messages: Cells lacking this gene express the exu locus genes (required for hexuronate utilization) constitutively. Its absence additionally confers the ability to grow on minimal medium plus glucuronate, which cells cannot do in the presence of ExuR.
messages: Induced by galacturonate, repressed by glucose.
messages: Transcriptional repressor for the exu locus which is required for galacturonate utilization.

224308:BSU 36810
gene id: 224308:BSU_36810
uniprot id: A0A6M3ZHE1
uniprot id: P37809
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase alpha/beta chains family.
messages: Cell membrane
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Membrane raft
messages: Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.

224308:BSU 04050
gene id: 224308:BSU_04050
uniprot id: A0A6M4JD18
uniprot id: P42963
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the LamB/PxpA family.
messages: Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
messages: Deletion mutant grows less well than wild type on minimal medium with ammonium as nitrogen source and cannot grow on 5-oxoproline. Mutant lacks 5-oxoprolinase activity and accumulates 5-oxo-L-proline.
messages: Forms a complex composed of PxpA, PxpB and PxpC.

224308:BSU 02940
gene id: 224308:BSU_02940
uniprot id: A0A6M3Z7N2
uniprot id: O34833
names: Similarity
messages: Belongs to the TelA family.

224308:BSU 38690
gene id: 224308:BSU_38690
uniprot id: P94371
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is sigma Y-dependent. Induced upon nitrogen starvation.

224308:BSU 08200
gene id: 224308:BSU_08200
uniprot id: A0A6M3Z8L5
uniprot id: P54715
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: By maltose; repressed by glucose.
messages: Cell membrane
messages: Cells lacking this gene show no maltose uptake.
messages: Maltose uptake in B.subtilis occurs only via the malP-encoded specific PTS-dependent EIICB(Mal) and not via another transport mechanism.
messages: Membrane
messages: The EIIC domain type-1 forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in maltose transport.

224308:BSU 14510
gene id: 224308:BSU_14510
uniprot id: P39760
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the KtrA potassium transport family.
messages: Catalytic subunit of the KtrCD potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.
messages: Cell membrane
messages: Homodimer, tetramer (dimer of homodimer) and octamer (tetramer of homodimer). Part of the KtrCD complex formed by an octameric catalytic ring of KtrC and a membrane associated dimer of KtrD forming a potassium channel (By similarity).
messages: Impaired potassium uptake.

224308:BSU 25720
gene id: 224308:BSU_25720
uniprot id: A0A6M4JNQ8
uniprot id: P54449
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TVP38/TMEM64 family.
messages: Cell membrane
messages: Membrane

224308:BSU 11070
gene id: 224308:BSU_11070
uniprot id: O06751
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 28230
gene id: 224308:BSU_28230
uniprot id: A0A6M3ZEQ1
uniprot id: P80698
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FKBP-type PPIase family. Tig subfamily.
messages: Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.
messages: Cytoplasm
messages: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase (By similarity).
messages: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.

224308:BSU 07690
gene id: 224308:BSU_07690
uniprot id: A0A6M3Z8G2
uniprot id: O34484
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.
messages: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.
messages: Cytoplasm
messages: Expressed at very low levels throughout growth.
messages: Monomer.
messages: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.

224308:BSU 26190
gene id: 224308:BSU_26190
uniprot id: P45916
names: Function
names: Similarity
names: Subunit
messages: Dimer of a small and a large subunit.
messages: May function as a terminase.
messages: To B.subtilis XtmB and phage SPP1 terminase large subunit.

224308:BSU 00620
gene id: 224308:BSU_00620
uniprot id: P37471
names: Function
messages: Required for vegetative and sporulation septum formation. Required for the activation of genes expressed under the control of the sporulation transcription factors sigma F and sigma E.

224308:BSU 22730
gene id: 224308:BSU_22730
uniprot id: A3F3D6
uniprot id: P31103
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the NDK family.
messages: Cytoplasm
messages: Homotetramer.
messages: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

224308:BSU 22820
gene id: 224308:BSU_22820
uniprot id: P50744
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00440
gene id: 224308:BSU_00440
uniprot id: P37466
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Expressed during both vegetative growth phase and sporulation.
messages: Mutants show poor germination in the presence of L-alanine and reduced biofilm formation.
messages: Spore core
messages: Stimulates biofilm formation via transcriptional activation of extracellular matrix genes. Acts by repressing SinR activity, independently of the SinI, SlrA and SlrR pathways. Could also be involved in the regulation of other genes during biofilm and spore formation.

224308:BSU 39430
gene id: 224308:BSU_39430
uniprot id: A0A6M4JQS2
uniprot id: P39140
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the SorC transcriptional regulatory family.
messages: Deoxyribose-5-phosphate is most likely the effector that modulates binding of DeoR to DNA.
messages: Homooctamer.
messages: Negative regulator of the dra-nupC-pdp operon. DeoR binds cooperatively to the operator DNA, which consists of a palindrome and a direct repeat sequence located 3' to the palindrome.

224308:BSU 24150
gene id: 224308:BSU_24150
uniprot id: A0A6M4JKS6
uniprot id: P45857
names: Developmental Stage
names: Induction
names: Similarity
messages: Belongs to the acyl-CoA dehydrogenase family.
messages: Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
messages: Subject to catabolite repression.

224308:BSU 14600
gene id: 224308:BSU_14600
uniprot id: A0A6M3ZFM9
uniprot id: P21883
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the 2-oxoacid dehydrogenase family.
messages: Binds 1 lipoyl cofactor covalently.
messages: Forms a 24-polypeptide structural core with octahedral symmetry.
messages: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity.
messages: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 01590
gene id: 224308:BSU_01590
uniprot id: A0A6M4JRA3
uniprot id: P55190
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 34570
gene id: 224308:BSU_34570
uniprot id: A0A6M3ZIU7
uniprot id: O06993
names: Function
names: Similarity
messages: Belongs to the glycosyl hydrolase 65 family.
messages: Catalyzes the phosphorolysis of maltose, leading to the formation of glucose and glucose 1-P.

224308:BSU 03410
gene id: 224308:BSU_03410
uniprot id: A0A6M3ZAF5
uniprot id: P42403
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyl hydrolase 1 family.
messages: Expressed at a low and constant level during growth, sporulation, and spore germination.
messages: Is able to catalyze the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source.
messages: Is not induced by aryl-beta-D-glucosides such as arbutin, salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG). Is not repressed by glucose.

224308:BSU 01830
gene id: 224308:BSU_01830
uniprot id: A0A6M4JC87
uniprot id: P39755
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family.
messages: Belongs to the inorganic carbon transporter (TC 9.A.2) DabB family.
messages: Cell membrane
messages: Forms a complex with DabA.
messages: Membrane
messages: Part of an energy-coupled inorganic carbon pump.

224308:BSU 04660
gene id: 224308:BSU_04660
uniprot id: A0A6M3ZAW3
uniprot id: P96622
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the PemK/MazF family.
messages: Homodimer (PubMed:14517982, PubMed:24120662). Forms a complex with antitoxin EndoAI in which the toxin activity is inhibited (PubMed:15882409). One dimer binds a ssRNA substrate, forms a heterohexamer composed of alternating toxin and antitoxin homodimers which inhibits the endoribonuclease activity. Antitoxin prevents RNA binding to the endoribonuclease (PubMed:24120662).
messages: Replacing all Arg residues by canavanine yields an enzyme that recognizes a longer consensus sequence UACAUA, thus altering its substrate specificity. Still cleaves between the first and second nucleotides.
messages: Toxic component of a type II toxin-antitoxin (TA) system.
messages: Toxic component of a type II toxin-antitoxin (TA) system. Specific for 5'-UACAU-3' sequences, cleaving after the first U (PubMed:21763692). Yields cleavage products with 3' phosphate and 5' hydroxyl groups (PubMed:15882409). Cannot digest substrate with a UUdUACAUAA cleavage site (PubMed:24120662). Overexpression is toxic for cell growth (shown in E.coli), probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli. The EndoA-EndoAI complex does not seem to bind its own promoter (PubMed:24120662).

224308:BSU 22600
gene id: 224308:BSU_22600
uniprot id: A0A6M3ZGQ2
uniprot id: P20691
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the EPSP synthase family.
messages: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 09420
gene id: 224308:BSU_09420
uniprot id: A0A6M4JGE0
uniprot id: P54421
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Cell wall hydrolase that cleaves gamma-D-glutamate-meso-diaminopimelate bonds in peptidoglycan (By similarity). Seems to play a role in cell separation during vegetative growth.
messages: Cells lacking this gene are about twice as long as those of the wild-type strain.
messages: Expressed during the exponential growth phase, under the control of SigA (major) and SigH (minor). Is also positively regulated by the two-component system YycFG.
messages: The N-terminal domain contains LysM domains that are thought to be involved in peptidoglycan binding, while the C-terminal domain is endowed with the catalytic activity.
messages: cell wall

224308:BSU 05880
gene id: 224308:BSU_05880
uniprot id: A0A6M3Z7Z2
uniprot id: O05512
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 26 family.
messages: Homodimer.
messages: Involved in the degradation of glucomannan. Catalyzes the endo hydrolysis of beta-1,4-linked mannan, galactomannan and glucomannan.
messages: Secreted
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 33221
gene id: 224308:BSU_33221
uniprot id: A0A6M3ZFX6
uniprot id: P94504
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YvrG/YvrH that positively regulates 7 transcriptional units (wprA, wapA-yxxG, dltABCDE, sunA, sunT-bdbA-yolJ-bdbB, sigO-rsoA, and sigX-rsiX), and negatively regulates the lytABC operon.
messages: Phosphorylated by YvrG.

224308:BSU 34640
gene id: 224308:BSU_34640
uniprot id: A0A6M4JPJ9
uniprot id: O06986
names: Similarity
messages: Belongs to the LOG family.

224308:BSU 26870
gene id: 224308:BSU_26870
uniprot id: A0A6M4JK06
uniprot id: O07906
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 29980
gene id: 224308:BSU_29980
uniprot id: O34944
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M20A family.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 36000
gene id: 224308:BSU_36000
uniprot id: A0A6M3ZH64
uniprot id: Q04777
names: Function
names: Induction
names: Similarity
messages: Belongs to the alpha-acetolactate decarboxylase family.
messages: Converts acetolactate into acetoin, which can be excreted by the cells. This may be a mechanism for controlling the internal pH of cells in the stationary stage.
messages: Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.

224308:BSU 06190
gene id: 224308:BSU_06190
uniprot id: O34434
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05160
gene id: 224308:BSU_05160
uniprot id: A0A6M3Z804
uniprot id: P96661
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 38160
gene id: 224308:BSU_38160
uniprot id: A0A6M4JQH0
uniprot id: P34956
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the heme-copper respiratory oxidase family.
messages: Binds a copper B center.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water. Major component for energy conversion during vegetative growth (By similarity).
messages: Cell membrane
messages: Heme A3.
messages: Membrane

224308:BSU 23350
gene id: 224308:BSU_23350
uniprot id: O32013
names: Similarity
messages: Belongs to the GerPA/GerPF family.

224308:BSU 34500
gene id: 224308:BSU_34500
uniprot id: A0A6M4JPG8
uniprot id: O06999
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 22140
gene id: 224308:BSU_22140
uniprot id: P50842
names: Function
names: Induction
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Catalyzes the reduction of 2,5-diketo-3-deoxygluconate (DKII or 4,6-dihydroxy-2,5-dioxohexanoate) into 2-keto-3-deoxygluconate (KDG or 2-dehydro-3-deoxygluconate) with a concomitant oxidation of NADH.
messages: Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.

224308:BSU 27630
gene id: 224308:BSU_27630
uniprot id: O32045
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 06970
gene id: 224308:BSU_06970
uniprot id: O31518
names: Function
names: Induction
names: Similarity
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: May play a role in the degradation of type I rhamnogalacturonan derived from plant cell walls.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 31200
gene id: 224308:BSU_31200
uniprot id: A0A6M4JNZ5
uniprot id: O05262
names: Caution
names: Function
names: Similarity
messages: Belongs to the rhamnulokinase family.
messages: Involved in the catabolism of L-rhamnose (6-deoxy-L-mannose). Catalyzes the transfer of the gamma-phosphate group from ATP to the 1-hydroxyl group of L-rhamnulose to yield L-rhamnulose 1-phosphate.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 15050
gene id: 224308:BSU_15050
uniprot id: A0A6M3ZAD6
uniprot id: O34470
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S16 family.
messages: Cell membrane

224308:BSU 24310
gene id: 224308:BSU_24310
uniprot id: A0A6M4JNE7
uniprot id: P54382
names: Function
names: Similarity
names: Subunit
messages: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
messages: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.
messages: Homodimer (By similarity). Interacts with BrxC(PubMed:33722570).
messages: Homodimer.

224308:BSU 10010
gene id: 224308:BSU_10010
uniprot id: O07515
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. TrpP subfamily.
messages: Cell membrane
messages: Cells lacking this gene grow on 40-fold higher concentrations of the tryptophan analog 5-fluorotryptophan than the level that inhibits the wild-type strain.
messages: Its translation is regulated by tryptophan-activated RNA-binding regulatory protein (TRAP).
messages: Probably involved in tryptophan uptake.

224308:BSU 05840
gene id: 224308:BSU_05840
uniprot id: A0A6M3Z8A5
uniprot id: O05508
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyl hydrolase 1 family.
messages: Expressed at only a very low level in exponential-phase cells and germinating spores, but is expressed at a higher levels upon entry into the stationary phase of growth.
messages: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in vivo.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR. Is not induced by aryl-beta-D-glucosides such as arbutin or salicin.

224308:BSU 03470
gene id: 224308:BSU_03470
uniprot id: P42406
names: Function
messages: Positive regulator of hxlAB expression.

224308:BSU 17150
gene id: 224308:BSU_17150
uniprot id: A0A6M3ZBB0
uniprot id: P40830
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thiolase-like superfamily. HMG-CoA synthase family.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac-AcpK) and a beta-ketothioester polyketide intermediate linked to one of the consecutive thiolation domains of PksL.

224308:BSU 17310
gene id: 224308:BSU_17310
uniprot id: O31793
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10240
gene id: 224308:BSU_10240
uniprot id: O07607
names: Cofactor
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 08840
gene id: 224308:BSU_08840
uniprot id: A0A6M3Z8S0
uniprot id: P40400
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein SsuA/TauA family.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system for aliphatic sulfonates. Putative binding protein.
messages: Periplasm
messages: Repressed by sulfate and cysteine.

224308:BSU 24440
gene id: 224308:BSU_24440
uniprot id: P49779
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 13020
gene id: 224308:BSU_13020
uniprot id: O34497
names: Similarity
messages: Belongs to the DDAH family.

224308:BSU 10070
gene id: 224308:BSU_10070
uniprot id: O07598
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M20A family.
messages: Binds 2 Zn(2+) or Co(2+) ions per subunit.

224308:BSU 13740
gene id: 224308:BSU_13740
uniprot id: A0A6M4JFL7
uniprot id: O31677
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.
messages: Binds 1 S-adenosyl-L-methionine per subunit.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 05230
gene id: 224308:BSU_05230
uniprot id: A0A6M3Z7W3
uniprot id: P96668
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 30150
gene id: 224308:BSU_30150
uniprot id: O32071
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 19600
gene id: 224308:BSU_19600
uniprot id: O34954
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 09910
gene id: 224308:BSU_09910
uniprot id: O07522
names: Cofactor
names: Induction
names: Similarity
messages: Belongs to the metallophosphoesterase superfamily.
messages: Binds 2 divalent metal cations.
messages: By mitomycin C and UV irradiation which requires RecA; probably a member of the yhaONM operon.

224308:BSU 02000
gene id: 224308:BSU_02000
uniprot id: A0A6M3Z7F9
uniprot id: O31432
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YbdK/YbdJ.
messages: Phosphorylated by YbdK.

224308:BSU 32890
gene id: 224308:BSU_32890
uniprot id: A0A6M3ZFV8
uniprot id: O32183
names: Similarity
messages: Belongs to the 4-oxalocrotonate tautomerase family.

224308:BSU 00710
gene id: 224308:BSU_00710
uniprot id: A0A6M3Z9P6
uniprot id: P37565
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the HSP33 family.
messages: Cytoplasm
messages: Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.
messages: Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive.

224308:BSU 36370
gene id: 224308:BSU_36370
uniprot id: A0A6M3ZL97
uniprot id: P94584
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioester dehydratase family. FabZ subfamily.
messages: Cytoplasm
messages: Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.

224308:BSU 23860
gene id: 224308:BSU_23860
uniprot id: A0A6M3ZDL7
uniprot id: P80859
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the 6-phosphogluconate dehydrogenase family.
messages: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
messages: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. Is the predominant 6-P-gluconate dehydrogenase isoenzyme in B.subtilis during growth on glucose and gluconate.
messages: Cells lacking this gene exhibit a reduced growth on glucose as the sole carbon source, and they do not use the pentose phosphate (PP) pathway at all.
messages: Homodimer.

224308:BSU 03060
gene id: 224308:BSU_03060
uniprot id: A0A6M3Z770
uniprot id: P55910
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the lactate permease family.
messages: Cell membrane
messages: May play a role in L-lactate transport.
messages: Membrane
messages: Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.
messages: Uptake of L-lactate across the membrane. Can also transport D-lactate and glycolate.

224308:BSU 40360
gene id: 224308:BSU_40360
uniprot id: A0A6M4JR73
uniprot id: P39668
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S1C family.
messages: Cell membrane
messages: Membrane

224308:BSU 11880
gene id: 224308:BSU_11880
uniprot id: A0A6M3Z9X5
uniprot id: O31632
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the trans-sulfuration enzymes family.
messages: Catalyzes the transformation of cystathionine into homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine.
messages: Cells lacking this gene grow as well as the wild-type in the presence of sulfate, cysteine, homocysteine or methionine as sole sulfur source, but do not grow in the presence of cystathionine.
messages: Cytoplasm
messages: Homotetramer.
messages: Up-regulated at the transcriptional level when sulfate, cysteine, cystathionine or homocysteine are the sulfur sources. Repressed by methionine.

224308:BSU 02590
gene id: 224308:BSU_02590
uniprot id: P42248
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 01750
gene id: 224308:BSU_01750
uniprot id: A0A6M3Z9Z6
uniprot id: Q45589
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the adenylate cyclase family. DacA/CdaA subfamily.
messages: Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.
messages: Cell membrane
messages: Constitutively expressed, part of the cdaA-cdaR-glmM-glmS operon (PubMed:23192352).
messages: DAC activity is stimulated about 20-fold in E.coli by coexpression with CdaR (PubMed:23192352).
messages: Increased sensitivity to the beta-lactam antibiotic cefuroxime (CEF), upon overexpression of the c-di-AMP phosphodiesterase GdpP greatly increased sensitivity to CEF (PubMed:22211522). Double disA-cdaA mutants cannot be made, suggesting they are lethal, while double disA-cdaS and cdaA-cdaS mutants are viable (PubMed:22211522, PubMed:23192352). Depletion of cdaA in double disA-cdaA deletion cells leads to cell lysis (PubMed:22211522). Exponentially growing cells are extremely sensitive to H(2)O(2), no change in response to methyl methanesulfonate (PubMed:25616256).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: One of 3 paralogous diadenylate cyclases (DAC) in this bacteria, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (Probable). Upon expression in E.coli leads to c-di-AMP synthesis (PubMed:23192352). Probably the main producer of c-di-AMP for the cell; is probably implicated in control of peptidogylcan synthesis (PubMed:22211522, PubMed:23192352, PubMed:26240071). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).
messages: Probably a homodimer (By similarity). Interacts with CdaR (PubMed:23192352, PubMed:26240071). May interact with GlmM (PubMed:26240071).
messages: Probably a homodimer.

224308:BSU 14050
gene id: 224308:BSU_14050
uniprot id: O34870
names: Cofactor
names: PTM
names: Similarity
messages: Belongs to the metallophosphoesterase superfamily.
messages: Binds 2 divalent metal cations.
messages: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

224308:BSU 06680
gene id: 224308:BSU_06680
uniprot id: A0A6M4JFT2
uniprot id: O06491
names: Function
names: Similarity
names: Subunit
messages: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
messages: Belongs to the amidase family. GatA subfamily.
messages: Heterotrimer of A, B and C subunits (PubMed:9342321). Interacts with BrxC (PubMed:33722570).
messages: Heterotrimer of A, B and C subunits.

224308:BSU 36120
gene id: 224308:BSU_36120
uniprot id: A0A6M4JPV3
uniprot id: O05216
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.
messages: Cell membrane
messages: Membrane

224308:BSU 36350
gene id: 224308:BSU_36350
uniprot id: P94586
names: Caution
messages: Could be the product of a pseudogene. The N-terminus is about 750 residues shorter than orthologs, and it contains a truncated response regulatory domain.

224308:BSU 34960
gene id: 224308:BSU_34960
uniprot id: O34993
names: Similarity
messages: Belongs to the transferase hexapeptide repeat family.

224308:BSU 03480
gene id: 224308:BSU_03480
uniprot id: A0A6M4JCW0
uniprot id: P27206
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 3 phosphopantetheines covalently.
messages: The phosphoserine observed at Ser-1006 in PubMed:17218307 undoubtedly results from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.
messages: This protein is a multifunctional enzyme able to activate and polymerize the amino acids Leu, Glu, Asp and Val. Activation sites for these AA consist of individual domains.

224308:BSU 32460
gene id: 224308:BSU_32460
uniprot id: A0A6M4JN47
uniprot id: O32142
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.
messages: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced by TnrA during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or uric acid are added during limiting-nitrogen conditions.
messages: HIU hydrolysis also occurs spontaneously, but more slowly.
messages: Homotetramer.

224308:BSU 07510
gene id: 224308:BSU_07510
uniprot id: A0A6M3Z8Z5
uniprot id: O34933
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of one ATP-binding protein (YfmF), two transmembrane proteins (YfmD and YfmE) and a solute-binding protein (YfmC).

224308:BSU 24700
gene id: 224308:BSU_24700
uniprot id: A0A6M4JJE5
uniprot id: P25956
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cell surface
messages: Processing of ComGD in competent cells requires ComC.
messages: Required for transformation and DNA binding.

224308:BSU 15840
gene id: 224308:BSU_15840
uniprot id: O34751
names: Disruption Phenotype
messages: No visible growth phenotype.

224308:BSU 30640
gene id: 224308:BSU_30640
uniprot id: A0A6M4JMF4
uniprot id: O34883
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacteriophage holin family. Cp-1 holin subfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 27000
gene id: 224308:BSU_27000
uniprot id: O06008
names: Disruption Phenotype
names: Function
names: Induction
messages: By aldehyde stress, positively regulates its own expression.
messages: Loss of expression of the adhA-yraA operon in response to formaldehyde and methylgloxal.
messages: Transcriptional regulator involved in the response to aldehyde stress. Binds to the promoter region of the adhA-yraA operon, the yraC and its own promoter region; binding is unchanged in the presence of aldehydes.

224308:BSU 38560
gene id: 224308:BSU_38560
uniprot id: A0A6M3ZK97
uniprot id: P46320
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyl hydrolase 4 family.
messages: Binds 1 NAD(+) per subunit.
messages: Hydrolyzes phospho-beta-glucosides.
messages: Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.

224308:BSU 16530
gene id: 224308:BSU_16530
uniprot id: A0A6M3ZBH6
uniprot id: O31751
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the UPP synthase family.
messages: Binds 2 magnesium ions per subunit.
messages: Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.
messages: Homodimer.

224308:BSU 27490
gene id: 224308:BSU_27490
uniprot id: O34452
names: Function
names: Subunit
messages: Could be an interacting mediator in the complex formation among RNA sulfuration components, RNA processing components, and aminoacyl-tRNA synthetases.
messages: Monomer.

224308:BSU 17770
gene id: 224308:BSU_17770
uniprot id: A0A6M4JJ27
uniprot id: O31810
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKC lipoprotein family.
messages: Cell membrane
messages: May be involved in spore germination.

224308:BSU 19420
gene id: 224308:BSU_19420
uniprot id: O31853
names: Similarity
messages: Belongs to the UDP-glycosyltransferase family.

224308:BSU 00360
gene id: 224308:BSU_00360
uniprot id: A0A6M3Z6L7
uniprot id: P37544
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyltransferase superfamily. RsmI family.
messages: Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.
messages: Cytoplasm

224308:BSU 22220
gene id: 224308:BSU_22220
uniprot id: P50830
names: Similarity
messages: Belongs to the helicase family.

224308:BSU 34540
gene id: 224308:BSU_34540
uniprot id: A0A6M3ZGI2
uniprot id: P80244
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase S14 family.
messages: By heat shock, salt stress, ethanol stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.
messages: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a limited peptidase activity in the absence of ATP-binding subunits ClpC, ClpE or ClpX (PubMed:20305655). Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). ClpXP is involved in the complete degradation of the site-2 clipped anti-sigma-W factor RsiW. This results in the release of SigW and the transcriptional activation of genes under the control of the sigma-W factor (PubMed:16899079). Probably the major protease that degrades proteins tagged by trans-translation (PubMed:11395451, PubMed:31155236).
messages: Cytoplasm
messages: Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes (PubMed:20305655, PubMed:22080375). Forms large heterooligomeric complexes consisting of an ATPase component (ClpX, ClpC or ClpE) and a proteolytic component (ClpP)(PubMed:20305655).
messages: Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.
messages: Low intrinsic peptidase activity is stimulated by ATP-binding subunits ClpC, ClpE and ClpX. Activity is disregulated by acyldepsipeptides (ADEP) antibiotics, which negate the need for ATP-binding subunits for activation and which makes it into an unregulated protease. Each ClpP subunit binds 1 ADEP molecule, which prevents binding of ClpX. ADEP binding causes conformational shifts that open the gated pore of the ring (PubMed:20305655). Protease activity is inhibited by diisopropylfluoro-phosphate (PubMed:22080375). Protease activity is inhibited by bortezomib, an oncology drug originally designed to work on the human proteasome (PubMed:31155236).
messages: No degradation of trans-translationally tagged-peptides (PubMed:11395451, PubMed:31155236). Triple rqcH clpP ssrA mutants cannot be generated (ssrA, or tmRNA, encodes the SsrA tag added to nascent proteins in stalled ribosomes by trans-translation, targeting the nascent protein for degradation) (PubMed:31155236).
messages: Peptide exit pores open in the compressed state; in this conformation the active site residues move apart and the protease cannot be active.

224308:BSU 06350
gene id: 224308:BSU_06350
uniprot id: P94476
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29130
gene id: 224308:BSU_29130
uniprot id: A0A6M4JLV7
uniprot id: P39126
names: Cofactor
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
messages: Binds 1 Mg(2+) or Mn(2+) ion per subunit.
messages: Homodimer.
messages: The enzyme can be phosphorylated in vitro by the E.coli isocitrate dehydrogenase kinase/phosphatase, but B.subtilis lacks such an enzyme.

224308:BSU 05370
gene id: 224308:BSU_05370
uniprot id: A0A6M3ZB25
uniprot id: P96681
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 18410
gene id: 224308:BSU_18410
uniprot id: A0A6M3ZBP5
uniprot id: P54422
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the gamma-glutamyltransferase family.
messages: Cleaved by autocatalysis into a large and a small subunit.
messages: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases.
messages: Expressed at the end of vegetative growth.
messages: Loss of ability to grow with glutathione as a sulfur source.
messages: Secreted
messages: This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.

224308:BSU 30810
gene id: 224308:BSU_30810
uniprot id: A0A6M4JKS7
uniprot id: P23974
names: Function
names: Similarity
names: Subunit
messages: Belongs to the AB hydrolase superfamily. MenH family.
messages: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC).
messages: Monomer.

224308:BSU 12320
gene id: 224308:BSU_12320
uniprot id: O34736
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LDH2/MDH2 oxidoreductase family.
messages: Cytoplasm
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 20460
gene id: 224308:BSU_20460
uniprot id: O31913
names: Similarity
messages: Belongs to the UPF0714 family.

224308:BSU 34140
gene id: 224308:BSU_34140
uniprot id: A0A6M4JPC6
uniprot id: O07011
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Involved in galactan degradation (PubMed:27501980). Part of the ABC transporter complex GanPQS involved in the uptake of galactooligosaccharides (PubMed:27501980, PubMed:29240795). Responsible for the translocation of the substrate across the membrane (Probable).
messages: Membrane
messages: Repressed by the transcriptional regulator GanR and induced by galactobiose. Also repressed by glucose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (GanP and GanQ) and a solute-binding protein (GanS).

224308:BSU 34390
gene id: 224308:BSU_34390
uniprot id: A0A6M4JPF9
uniprot id: P37967
names: Function
names: Similarity
names: Subunit
messages: Belongs to the type-B carboxylesterase/lipase family.
messages: Catalyzes hydrolysis of several beta-lactam antibiotic PNB esters to the corresponding free acid and PNB alcohol.
messages: Monomer.

224308:BSU 01600
gene id: 224308:BSU_01600
uniprot id: P55192
names: Induction
messages: Induced by Btr in iron-limited conditions.

224308:BSU 11000
gene id: 224308:BSU_11000
uniprot id: O06744
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 37220
gene id: 224308:BSU_37220
uniprot id: P45862
names: Similarity
names: Subcellular Location Annotation
messages: Membrane
messages: To B.subtilis YyaP.

224308:BSU 10710
gene id: 224308:BSU_10710
uniprot id: O06720
names: Developmental Stage
names: Function
names: Induction
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 38130
gene id: 224308:BSU_38130
uniprot id: P39603
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YwcE family.
messages: Cell membrane
messages: Expressed at the onset of sporulation.
messages: Repressed by AbrB during growth and activated at the onset of sporulation in a Spo0A-dependent manner.
messages: Required for proper spore morphogenesis. Important for spore germination.
messages: Spore membrane
messages: Spore outer membrane

224308:BSU 29040
gene id: 224308:BSU_29040
uniprot id: A0A6M4JN14
uniprot id: O34367
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 09190
gene id: 224308:BSU_09190
uniprot id: P54602
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: In the C-terminal section; belongs to the 5'-nucleotidase family.
messages: Requires a minimum of 0.1 mM of calcium for a significant activity. Maximal activity was observed with concentrations of calcium between 1 to 5 mM. Is 10-fold less active with the corresponding concentrations of manganese. Inhibited by NaCl at concentrations of 100 mM and higher.
messages: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values.
messages: cell wall

224308:BSU 19320
gene id: 224308:BSU_19320
uniprot id: Q796C3
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the terpene cyclase/mutase family.
messages: Catalyzes the cyclization of tetraprenyl beta-curcumene into sporulenol.
messages: Cell membrane
messages: No effect on biofilm formation.

224308:BSU 17559
gene id: 224308:BSU_17559
uniprot id: C0H415
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38330
gene id: 224308:BSU_38330
uniprot id: A0A6M4JQM8
uniprot id: P39590
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family.
messages: Cell membrane
messages: Membrane

224308:BSU 12850
gene id: 224308:BSU_12850
uniprot id: A0A6M3Z9Q4
uniprot id: O34454
names: Similarity
messages: Belongs to the UPF0111 family.

224308:BSU 18590
gene id: 224308:BSU_18590
uniprot id: O31830
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0715 family.
messages: Cell membrane

224308:BSU 40070
gene id: 224308:BSU_40070
uniprot id: P12012
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GntP permease family.
messages: Cell membrane

224308:BSU 12630
gene id: 224308:BSU_12630
uniprot id: P54329
names: Similarity
messages: To B.subtilis YqbI.

224308:BSU 04580
gene id: 224308:BSU_04580
uniprot id: A0A6M4JD28
uniprot id: P96614
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DEAD box helicase family. CshA subfamily.
messages: Cell membrane
messages: Cytoplasm
messages: DEAD-box RNA helicase possibly involved in RNA degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA-dependent ATPase activity.
messages: Homodimer or oligomer. May interact with RNA helicases CshB and DbpA (DeaD). Probably a component of the RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno, and possibly also rnpA (although rnjA and rnjB's presence is unclear). Interacts with ribosomal proteins L1 and L3 (rplA and rplC) and the protein component of RNase RnpA. Interacts with the RNA polymerase core (PubMed:21710567).
messages: Induced by cold shock (PubMed:12399512). Constitutively expressed at 37 and 16 degrees Celsius in rich and minimal medium and in exponential, transition and stationary phase (at protein level) (PubMed:20572937, PubMed:23175651). Protein level not increased at 16 degrees Celsius (at protein level) (PubMed:20572937).
messages: Oligomerizes, may be a member of the RNA degradosome.
messages: RNA helicase activity is inhibited by EDTA.
messages: Slow vegetative growth at 37 degrees Celsius, impaired growth at 22 degrees Celsius (PubMed:16861794) and 16 degrees Celsius (PubMed:23175651). Another report shows no growth difference at 15 degrees Celsius (PubMed:16352840). The presence of CshA or CshB is essential for viability; in a cshA disruption mutant further depletion of cshB stops growth after 1 cell duplication (PubMed:16352840). Others show a quadruple disruption of all RNA helicases (cshA, cshB, deaD, yfmL) was not lethal at 37 degrees Celsius, although both 50S and 70S ribosomes are decreased, growth stops at 16 degrees (PubMed:23175651). At 20 degrees Celsius cells are elongated and wrinkled, with smaller cell diameter and thickened walls, and decreased amounts of 70S and 50S ribosomes; levels of over 200 transcripts are altered (PubMed:23175651).
messages: The C-terminal half of the protein (residues 225-494) is required for interaction with most RNA degradosome partners, whereas dimerization or oligomerization only requires the extreme C-terminus (residues 413-494). Addition of the latter domain to CshB confers on it the ability to interact with Rny.
messages: The most abundant DEAD-box RNA helicase. An ATP-dependent RNA helicase with RNA-dependent ATPase activity. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. In vitro, unwinds dsRNA in both 5'- and 3'- directions. Plays a role in ribosomal 50S subunit assembly. Its deletion leads to changes in mRNA levels for over 200 transcripts.
messages: nucleoid

224308:BSU 17490
gene id: 224308:BSU_17490
uniprot id: O31800
names: Similarity
messages: Belongs to the UPF0457 family.

224308:BSU 34900
gene id: 224308:BSU_34900
uniprot id: A0A6M3ZHD3
uniprot id: O34683
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the imidazoleglycerol-phosphate dehydratase family.
messages: Cytoplasm

224308:BSU 15660
gene id: 224308:BSU_15660
uniprot id: A0A6M3ZAI7
uniprot id: O34441
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the YicC/YloC family.
messages: Endonucleolytically cleaves ssRNA generating a 3'-OH and (presumably) a 5'-phosphate group. Cleaves preferentially at U residues, has no activity on dsRNA or dsDNA. Upon expression in E.coli contributes to the degradation of small RNA (sRNA) RhyB.
messages: Mn(2+) gives highest endonuclease activity in vitro, followed by Mg(2+) and to a lesser extent by Co(2+).
messages: No visible phenotype, no effect on sporulation. A quintuple ribonuclease deletion mutant (pnp, rph, rnr, yhaM, yloC) is viable.
messages: Oligomerizes, perhaps as a hexamer (PubMed:34815358). Might interact with PNPase (pnp) in E.coli (PubMed:34815358) (Probable).

224308:BSU 10850
gene id: 224308:BSU_10850
uniprot id: P40332
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.
messages: Catalyzes the reversible NAD(+)-dependent oxidation of scyllo-inositol (SI) to 2,4,6/3,5-pentahydroxycyclohexanone (scyllo-inosose or SIS). Is required for SI catabolism that allows B.subtilis to utilize SI as the sole carbon source for growth. Cannot use NADP(+) instead of NAD(+).
messages: Inactivation of this gene abolishes growth on SI as the sole carbon source, but does not affect growth on MI or glucose.
messages: Induced by either myo-inositol (MI) or SI; SI is approximately three times more efficient than MI.

224308:BSU 33930
gene id: 224308:BSU_33930
uniprot id: A0A6M3ZH21
uniprot id: P40924
names: Caution
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the phosphoglycerate kinase family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 18120
gene id: 224308:BSU_18120
uniprot id: A0A6M3ZBL9
uniprot id: Q45068
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.
messages: Cell membrane
messages: Membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 32340
gene id: 224308:BSU_32340
uniprot id: O32130
names: Cofactor
names: Developmental Stage
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the peptidase M23B family.
messages: Binds 1 zinc ion per subunit.
messages: Expressed only during sporulation.
messages: Expressed under the control of the late mother cell-specific sigma factor sigma-K.
messages: L-Ala--D-Glu endopeptidase involved in production of single L-alanine side chains from tetrapeptides in the spore cortex peptidoglycan. Therefore, is required for the endospore cortex maturation.
messages: The presence of single L-alanine residues in the spore cortex increases the spores ability to be resistant to heat.

224308:BSU 04730
gene id: 224308:BSU_04730
uniprot id: P06574
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the sigma-70 factor family. SigB subfamily.
messages: By heat shock, salt stress, oxidative stress, glucose limitation, oxygen limitation and entry into stationary phase. Association with RNAP core increases during alcohol, NaOH, NaCl stress and during sporulation (at protein level) (PubMed:21710567).
messages: Interacts transiently with the RNAP core.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcribed in the early stationary phase under conditions inimical to sporulation. May play a role in the ability of the bacterium to adapt to various stresses but is not essential for its survival under these conditions. Positively regulates expression of its own operon. The second most abundant sigma factor, it associates with RNAP core under all growth phases (PubMed:21710567).

224308:BSU 33260
gene id: 224308:BSU_33260
uniprot id: A0A6M3ZG83
uniprot id: P46324
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Membrane

224308:BSU 30370
gene id: 224308:BSU_30370
uniprot id: A0A6M4JN68
uniprot id: O34741
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Expression is induced by bacitracin, via the two-component regulatory system BceS/BceR.
messages: Membrane
messages: Part of the ABC transporter complex BceAB (TC 3.A.1.123.5) involved in bacitracin export.
messages: The complex is composed of two ATP-binding proteins (BceA) and two transmembrane proteins (BceB).

224308:BSU 02420
gene id: 224308:BSU_02420
uniprot id: A0A6M4JFL2
uniprot id: O31464
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.
messages: Cell membrane
messages: Membrane
messages: Probably functions as a sodium/glutamine symporter for glutamine uptake.

224308:BSU 33480
gene id: 224308:BSU_33480
uniprot id: O32218
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: A late competence gene, expression is enhanced in the presence of ComK.
messages: Belongs to the thioredoxin family. DsbA subfamily.
messages: Cell membrane
messages: Cells partially restore cytochrome c oxidase activity in a CcdA-deficient mutant, possibly because the bacteria can no longer oxidize the 2 heme-binding thiol groups in apocytochrome c.
messages: Membrane raft
messages: Required for the stabilization, possibly via formation of a disulfide bond, of the obligatory competence protein ComGC. May be required for the stability of secreted proteins with disulfide bonds. Not required for sporulation.

224308:BSU 28020
gene id: 224308:BSU_28020
uniprot id: A0A6M3ZFA8
uniprot id: Q01466
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MreC family.
messages: Cell membrane
messages: Cell septum
messages: Involved in formation and maintenance of cell shape.
messages: Involved in formation and maintenance of cell shape. Required for the formation of proper helical filaments of MreB and for cell wall synthesis in the cylindrical part of the cell leading to cell elongation.
messages: Loss of viability. Cells lacking sufficient quantities of this protein undergo morphological changes, namely, swelling and twisting of the cells followed by cell lysis. A polymeric material accumulates at one side of the division septum of the cells and the presence of this material correlates with the bending of the cell. After prolonged depletion in the presence of MgCl(2), the cells become very short and fat and eventually spheroidal, but no significant lysis is observed. Addition of sucrose results in rounded cells. Cells are still capable of making division septa. Null mutants show little or no sign of a defined long axis and have a severe shape defect.
messages: Membrane raft
messages: Self-associates. Interacts with MreD and proteins that belong to class A and class B high-molecular-weight penicillin-binding proteins (PBP2b, PBP1, PBP2a, PBP3, PBP4, PBP2c, PBP2d, PBPH and PBP4b).

224308:BSU 28170
gene id: 224308:BSU_28170
uniprot id: A0A6M3ZEU1
uniprot id: P16618
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the glutamyl-tRNA reductase family.
messages: Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).
messages: During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.
messages: Homodimer.
messages: Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.

224308:BSU 18760
gene id: 224308:BSU_18760
uniprot id: A0A6M3ZBT0
uniprot id: O34701
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 06110
gene id: 224308:BSU_06110
uniprot id: O34303
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CTCGAG-3'; the cleavage site is unknown.
messages: BsuMI restriction activity requires YdiR, YdiS and YdjA.
messages: Constitutively expressed during exponential growth. Encoded in an operon with ydiR and ydiS.
messages: Not essential; its disruption results in increased transformation by plasmid DNA carrying multiple BsuMI target sequences (PubMed:11751814). Triple deletion ydiO-ydiP-ydjA leads to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).
messages: Not expressed during sporulation.

224308:BSU 33800
gene id: 224308:BSU_33800
uniprot id: A0A6M3ZGQ4
uniprot id: O34742
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Repressed by OpcR.
messages: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).

224308:BSU 11440
gene id: 224308:BSU_11440
uniprot id: A0A6M3ZA17
uniprot id: P24138
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Membrane
messages: Membrane raft
messages: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 40260
gene id: 224308:BSU_40260
uniprot id: Q45605
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 12560
gene id: 224308:BSU_12560
uniprot id: P39784
names: Function
messages: Positive regulatory protein that acts at the late promoter PL.

224308:BSU 32020
gene id: 224308:BSU_32020
uniprot id: O32103
names: Cofactor
messages: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.

224308:BSU 07810
gene id: 224308:BSU_07810
uniprot id: A0A6M3Z8J1
uniprot id: P39795
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Activity is stimulated by high salt concentrations with different efficiencies depending on the kind of salt. In vitro, inhibited by glucose.
messages: Belongs to the glycosyl hydrolase 13 family.
messages: Cytoplasm
messages: Hydrolyzes trehalose-6-phosphate to glucose and glucose 6-phosphate. Can also very effectively hydrolyze p-nitrophenyl-alpha-D-glucopyranoside, but not lactose, maltose, sucrose or sucrose-6-phosphate. Trehalose is also hydrolyzed, but to a much smaller extent than trehalose-6-phosphate.
messages: Induced by trehalose and repressed by glucose, fructose or mannitol.

224308:BSU 17040
gene id: 224308:BSU_17040
uniprot id: A0A6M3ZB00
uniprot id: P49849
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the DNA mismatch repair MutS family.
messages: Cells lacking this gene have a 225-fold increased spontaneous mutation frequency. Double or triple disruptions with mutL/nth do not change the frequency.
messages: This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity (By similarity). Overexpression of mutSL partially suppresses the high spontaneous mutation frequency of a ytkD/mutM/mutY triple disruption which lacks the system required to prevent damage by oxidized guanine (8-oxo-dGTP). This suggests that MutSL also functions to repair mismatches due to oxidative stress in both growing and stationary phase cells.
messages: This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

224308:BSU 11310
gene id: 224308:BSU_11310
uniprot id: O32437
names: Function
messages: Negatively regulates the transcription of the comG operon.

224308:BSU 29640
gene id: 224308:BSU_29640
uniprot id: O34553
names: Similarity
messages: Belongs to the free Met sulfoxide reductase family.

224308:BSU 22520
gene id: 224308:BSU_22520
uniprot id: P54393
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 27130
gene id: 224308:BSU_27130
uniprot id: O05403
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Anti-sigma factor for SigV. Negatively regulates SigV activity through direct interaction.
messages: Belongs to the RsiV family.
messages: Cell membrane
messages: Interacts (via N-terminal region) with SigV.

224308:BSU 02700
gene id: 224308:BSU_02700
uniprot id: P37957
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Active toward triacylglycerides with a preference for esters with C8:0 acyl groups; barely active on C18:1 or C18:4 substrates. Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18.
messages: Belongs to the AB hydrolase superfamily.
messages: Maximally expressed in late exponential growth phase. Expression decreases rapidly in the stationary phase. Expressed in both rich and minimal media with glucose as carbon source (at protein level).
messages: Monomer.
messages: Secreted
messages: Strongly inhibited when incubated with the serine reagent phenylmethylsulfonyl fluoride. Activated by the addition of calcium to the reaction mixture. When calcium was incubated with the lipase but not added to the reaction mixture, its effect is lower but still observable. Magnesium, manganese and strontium are not able to replace calcium with full retention of activity.

224308:BSU 15460
gene id: 224308:BSU_15460
uniprot id: A0A6M3ZAG7
uniprot id: Q45480
names: Function
names: Similarity
messages: Belongs to the pseudouridine synthase RluA family.
messages: Responsible for synthesis of pseudouridine from uracil.

224308:BSU 39510
gene id: 224308:BSU_39510
uniprot id: P54951
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the acetyltransferase family.
messages: Catalyzes the N-acetylation of S-(2-succino)cysteine. Is involved in a S-(2-succino)cysteine (2SC) degradation pathway that allows B.subtilis to grow on 2SC as a sole sulfur source, via its metabolization to cysteine. Moreover, 2SC is a toxic compound in B.subtilis at high exogenous concentrations, and this enzyme relieves 2SC toxicity via N-acetylation.
messages: Cells lacking this gene almost lose the ability to grow on 2SC as the sulfur source but are still able to grow on sulfate. Moreover, in contrast to wild type, they highly accumulate 2SC when grown on fumarate as the sulfur source. Addition of 2SC causes growth inhibition of the deletion mutant but not the wild type grown on sulfate as the sulfur source.
messages: Fumarate mediated succination of thiols increases in certain tumors and in response to glucotoxicity associated with diabetes. Therefore, S-(2-succino)-adducts such as S-(2-succino)cysteine (2SC) are considered oncometabolites and biomarkers for human disease. The demonstration of a metabolic disposal route for a S-(2-succino)-compound paves the way toward the identification of corresponding pathways in other species.

224308:BSU 29940
gene id: 224308:BSU_29940
uniprot id: A0A6M4JKZ2
uniprot id: O34799
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the diacylglycerol/lipid kinase family.
messages: Binds 1 Mg(2+) ion per subunit. This ion appears to have a structural role and is required for catalytic activity.
messages: May catalyze the ATP-dependent phosphorylation of lipids other than diacylglycerol (DAG). In fact, is not able to exhibit diacylglycerol kinase activity in vitro.

224308:BSU 15160
gene id: 224308:BSU_15160
uniprot id: A0A6M3ZAJ3
uniprot id: Q07868
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: Essential, it cannot be deleted (PubMed:8244929, PubMed:8636036). Depletion leads to an arrest of cell division after 80 minutes at 37 degrees Celsius and 40 minutes at 48 degrees Celsius with a concomitant reduction in FtsL and to a lesser extent DivIC levels; cells lengthen after arrest and eventually lyse (PubMed:16936019, PubMed:28792086).
messages: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis (Probable). This protein itself, but not its transpeptidase activity, is required for cell division (PubMed:28792086).
messages: Synthesized throughout vegetative growth. Synthesis is enhanced during stage II of sporulation and in stage IV mother cells. Undetectable in stage IV forespores. Present in the inner forespore membrane of the dormant spore.
messages: Transcribed at a low constant level in all growth phases. Part of the mraZ-rsmH-ftsL-pbpB operon.

224308:BSU 12080
gene id: 224308:BSU_12080
uniprot id: A0A6M4JH25
uniprot id: O31652
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
messages: Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.
messages: Cell membrane
messages: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.
messages: Interacts with CtaA.
messages: Membrane

224308:BSU 21650
gene id: 224308:BSU_21650
uniprot id: O32005
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39780
gene id: 224308:BSU_39780
uniprot id: P46336
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 11 subfamily.
messages: Disruption of this gene affects neither growth on inositol nor the inducibility and catabolite repression of Idh synthesis.
messages: In vitro, is able to reduce the standard aldo-keto reductase (AKR) substrates DL-glyceraldehyde, D-erythrose and methylglyoxal in the presence of NADPH, albeit with poor efficiency. Shows only trace activity with benzaldehyde and butyraldehyde. Is unable to oxidize myo-inositol with either NADP(+) or NAD(+) as a cosubstrate and also does not use glucose, 2-pyridine carboxyaldehyde, fructose, xylose and succinyl semialdehyde as a substrate (PubMed:12554958, PubMed:15019785). The physiological function of this enzyme is not clear (PubMed:15019785). Does not seem to be necessary for inositol catabolism (PubMed:9226270).
messages: Induced by inositol (PubMed:9226270). Repressed by glucose (PubMed:11160890). Is cotranscribed with iolR, the repressor of the iol operon (PubMed:9226270).
messages: Monomer.
messages: The 3D-structure shows a unique architecture for an AKR active site, which may explain the low catalytic power.

224308:BSU 26620
gene id: 224308:BSU_26620
uniprot id: A0A6M4JK80
uniprot id: O07086
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 31450
gene id: 224308:BSU_31450
uniprot id: Q08430
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the KinB activity.

224308:BSU 15290
gene id: 224308:BSU_15290
uniprot id: A0A6M3ZFU4
uniprot id: P17865
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsZ family.
messages: Cytoplasm
messages: During sporulation, is negatively regulated by MciZ, which binds to FtsZ and inhibits its polymerization and the formation of the Z ring.
messages: Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.
messages: Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner (By similarity). Interacts directly with several other division proteins (By similarity). Interacts with FtsA (PubMed:16159787). Interacts with Phi29 DNA replication protein 1 (PubMed:23836667). Interacts with the cell division inhibitor MciZ (PubMed:23836667, PubMed:25848052).
messages: Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.

224308:BSU 40440
gene id: 224308:BSU_40440
uniprot id: A0A6M3ZIK0
uniprot id: P37469
names: Function
names: Similarity
messages: Belongs to the helicase family. DnaB subfamily.
messages: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.

224308:BSU 23440
gene id: 224308:BSU_23440
uniprot id: P40866
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 03790
gene id: 224308:BSU_03790
uniprot id: A0A6M3Z7F5
uniprot id: P94417
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aspartokinase family.
messages: Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.
messages: Monomer.

224308:BSU 22530
gene id: 224308:BSU_22530
uniprot id: P54392
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29080
gene id: 224308:BSU_29080
uniprot id: A0A6M3ZFC7
uniprot id: O34403
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the FPG family.
messages: Binds 1 zinc ion per subunit.
messages: Cells lacking this gene have a 5-fold increased spontaneous mutation frequency. A double mutM/mutY mutant has a 1000-fold increased spontaneous mutation frequency (PubMed:12483591). Triple ytkD/mutM/mutY disrupted strains show increased mutation rates during both exponential and stationary phase (PubMed:19011023).
messages: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
messages: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions (By similarity). Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 8-oxo-dGTP. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 33400
gene id: 224308:BSU_33400
uniprot id: O32210
names: Function
names: Similarity
messages: Belongs to the aldo/keto reductase family.
messages: Reduces glyoxal and methylglyoxal (2-oxopropanal). Is not involved in the vitamin B6 biosynthesis.

224308:BSU 22480
gene id: 224308:BSU_22480
uniprot id: A0A6M4JNJ8
uniprot id: P42980
names: Function
names: Similarity
messages: Belongs to the methylglyoxal synthase family.
messages: Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.

224308:BSU 37230
gene id: 224308:BSU_37230
uniprot id: A0A6M3ZHG6
uniprot id: P45861
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane
messages: Membrane raft

224308:BSU 02710
gene id: 224308:BSU_02710
uniprot id: A0A6M4JEW1
uniprot id: O31469
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 07560
gene id: 224308:BSU_07560
uniprot id: A0A6M4JL16
uniprot id: P39116
names: Cofactor
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the polysaccharide lyase 1 family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Monomer.
messages: Negatively regulated by TnrA under nitrogen-limited conditions.
messages: Produced during the exponential death phase of growth, just before sporulation.
messages: Produces unsaturated products from polygalacturonate.
messages: Secreted

224308:BSU 21900
gene id: 224308:BSU_21900
uniprot id: A0A6M3ZD33
uniprot id: P52035
names: Similarity
messages: Belongs to the glutathione peroxidase family.

224308:BSU 01530
gene id: 224308:BSU_01530
uniprot id: P50864
names: Developmental Stage
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
messages: Cleaves the peptide side chain from the N-acetylmuramic acid residues in peptidoglycan. This is a step in the formation of muramic delta-lactam residues in spore cortex.
messages: CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.
messages: Expression of cwlD takes place in both the mother cell and forespore compartments of sporulating cells.
messages: Secreted

224308:BSU 36780
gene id: 224308:BSU_36780
uniprot id: O32278
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 04240
gene id: 224308:BSU_04240
uniprot id: A0A6M3Z7S2
uniprot id: O31485
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 00960
gene id: 224308:BSU_00960
uniprot id: Q06753
names: Similarity
messages: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.

224308:BSU 17290
gene id: 224308:BSU_17290
uniprot id: A0A6M4JIJ2
uniprot id: P0CW82
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. EbrA/EbrB subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons (By similarity).
messages: The efflux pump is composed of EbrA and EbrB.

224308:BSU 26960
gene id: 224308:BSU_26960
uniprot id: A0A6M4JK16
uniprot id: O07949
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Spore coat

224308:BSU 35390
gene id: 224308:BSU_35390
uniprot id: P96502
names: Induction
messages: Transcriptionally regulated by SigD.

224308:BSU 25200
gene id: 224308:BSU_25200
uniprot id: A0A6M4JLH7
uniprot id: P06224
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A conformational change of sigma-A occurs during its association with the core enzyme.
messages: Association with RNAP core remains approximately constant during all stresses tested and during sporulation (at protein level).
messages: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
messages: Cytoplasm
messages: Interacts transiently with the RNA polymerase catalytic core.
messages: Interacts transiently with the RNAP core.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. This sigma factor is the primary sigma factor during all growth phases, stress and during sporulation. It associates with the RNAP core at all growth stages with a slight increase in stationary phase.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.

224308:BSU 17160
gene id: 224308:BSU_17160
uniprot id: A0A6M3ZGD2
uniprot id: P40805
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the enoyl-CoA hydratase/isomerase family.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably catalyzes the dehydration of the (S)-3-hydroxy-3-methylglutaryl group attached to PksL.

224308:BSU 13380
gene id: 224308:BSU_13380
uniprot id: O34830
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32270
gene id: 224308:BSU_32270
uniprot id: O32123
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotS family.
messages: Forespore outer membrane
messages: Involved in sporulation.
messages: Spore coat

224308:BSU 05350
gene id: 224308:BSU_05350
uniprot id: P96679
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 39710
gene id: 224308:BSU_39710
uniprot id: A0A6M4JQX0
uniprot id: P42417
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane
messages: Minor myo-inositol uptake transporter.
messages: The growth-defect caused by the iolF inactivation was only observed in the iolT-null background.

224308:BSU 29960
gene id: 224308:BSU_29960
uniprot id: A0A6M4JNR3
uniprot id: O34570
names: Function
names: Similarity
messages: Belongs to the 2H phosphoesterase superfamily. ThpR family.
messages: Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.

224308:BSU 13130
gene id: 224308:BSU_13130
uniprot id: A0A6M4JJ65
uniprot id: P39821
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the gamma-glutamyl phosphate reductase family.
messages: Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
messages: Cytoplasm

224308:BSU 32910
gene id: 224308:BSU_32910
uniprot id: O32185
names: Caution
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Could be the product of a pseudogene. This sequence is shorter than orthologs and lacks the conserved active site Tyr residue.

224308:BSU 39400
gene id: 224308:BSU_39400
uniprot id: A0A6M4JQR2
uniprot id: P39142
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
messages: Binds 1 K(+) ion per subunit.
messages: Catalyzes phosphorolysis of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and ribose-1-phosphate.
messages: Homodimer.
messages: Induced by deoxyadenosine and thymidine. Repressed by DeoR and glucose.

224308:BSU 23810
gene id: 224308:BSU_23810
uniprot id: P54551
names: Similarity
messages: To B.subtilis RocB.

224308:BSU 13540
gene id: 224308:BSU_13540
uniprot id: A0A6M3ZAE0
uniprot id: P11742
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Bacillus subtilis contains 2 species of O-6-methylguanine-DNA alkyltransferases. Ogt and adaB are regulated in different ways, constitutively and inducibly on adaptive treatment, respectively.
messages: Belongs to the MGMT family.
messages: Constitutively expressed.
messages: Cytoplasm
messages: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
messages: This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

224308:BSU 22990
gene id: 224308:BSU_22990
uniprot id: P50732
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00210
gene id: 224308:BSU_00210
uniprot id: A0A6M4JDW2
uniprot id: P24277
names: Function
names: Similarity
messages: Belongs to the RecR family.
messages: May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.

224308:BSU 15270
gene id: 224308:BSU_15270
uniprot id: A0A6M3ZE26
uniprot id: P28265
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sbp family.
messages: Cell membrane

224308:BSU 08140
gene id: 224308:BSU_08140
uniprot id: O31555
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To B.subtilis YfjE.

224308:BSU 14690
gene id: 224308:BSU_14690
uniprot id: A0A6M3ZFN8
uniprot id: Q45500
names: Function
names: Similarity
messages: Belongs to the UPF0677 family.
messages: Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.
messages: May be involved in polyketide synthesis.

224308:BSU 39550
gene id: 224308:BSU_39550
uniprot id: P54947
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.

224308:BSU 08460
gene id: 224308:BSU_08460
uniprot id: A0A6M4JG64
uniprot id: O31569
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Induced by iron starvation, repressed by fur.
messages: Membrane
messages: Part of the ABC transporter complex YfiYZ/YfhA/YusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen.
messages: Strains lacking this gene show a reduction in growth stimulation by the iron-hydroxamate siderophores schizokinen and arthrobactin compared to wild-type.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (YfiZ and YfhA) and a solute-binding protein (YfiY).

224308:BSU 31790
gene id: 224308:BSU_31790
uniprot id: O32094
names: Similarity
messages: Belongs to the GerPA/GerPF family.

224308:BSU 34610
gene id: 224308:BSU_34610
uniprot id: O06989
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Induced by maltose and repressed by glucose.
messages: Inhibited by glucose and lactose.
messages: Maltodextrin can also be transported inside the cell after degradation to maltose by extracellular amylase AmyE, transported as maltose by MalP.
messages: Part of the ABC transporter complex involved in maltodextrin import. Binds maltodextrin. Can also bind maltose with low affinity, but is not involved in its uptake.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MdxF and MdxG) and a solute-binding protein (MdxE).

224308:BSU 37390
gene id: 224308:BSU_37390
uniprot id: P71009
names: Function
names: Induction
names: Similarity
messages: Belongs to the ABC transporter superfamily.
messages: Involved in the production of the bacteriocin subtilosin. Required for immunity to subtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 24850
gene id: 224308:BSU_24850
uniprot id: A0A6M3ZEF6
uniprot id: P54495
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: An ATP-dependent kinase that phosphorylates glucose to make glucose-6-phosphate, not active on fructose, galactose or mannose. Restores glucose kinase activity in E.coli glk mutants.
messages: Belongs to the ROK (NagC/XylR) family.
messages: Constitutively expressed, activity is maximal at the end of vegetative growth and declines during stationary phase when grown in rich broth (LB) (at protein level). Expressed in a similar fashion in minimal medium containing glucose, fructose, trehalose, maltose or sucrose.
messages: Cytoplasm
messages: Loss of glucokinase activity, no growth phenotype on glucose, fructose, maltose, sucrose or trehalose-containing minimal media.

224308:BSU 00840
gene id: 224308:BSU_00840
uniprot id: P37569
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Subunit
messages: Activates the phosphorylation activity of the protein-arginine kinase McsB. Is required for the delocalization of competence proteins from the cell poles.
messages: Cells lacking this gene show a defect in the delocalization of competence proteins.
messages: Interacts with McsB.
messages: Is repressed by the transcriptional regulator CtsR. Forms part of an operon with ctsR, mcsB and clpC.
messages: Phosphorylated on Arg residues by McsB.

224308:BSU 08230
gene id: 224308:BSU_08230
uniprot id: A0A6M4JGM0
uniprot id: P54720
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DoxX family.
messages: Cell membrane
messages: Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol.
messages: Membrane
messages: Strongly induced by catechol, less strongly by 2-methylhydroquinone (2-MHQ) but only weakly by chromanon (6-brom-2-vinyl-chroman-4-on).

224308:BSU 09640
gene id: 224308:BSU_09640
uniprot id: A0A6M3ZE83
uniprot id: O07594
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MscS (TC 1.A.23) family.
messages: Cell membrane
messages: Cells lacking this gene grow normally in minimal medium or in high-osmolarity environments, and exhibit the same survival capacity as the wild-type strain upon a drastic osmotic downshift. They sporulate and germinate normally.
messages: May play a role in resistance to osmotic downshock.
messages: Membrane

224308:BSU 06330
gene id: 224308:BSU_06330
uniprot id: P94474
names: Similarity
messages: Belongs to the MoxR family.

224308:BSU 23690
gene id: 224308:BSU_23690
uniprot id: P54562
names: Subunit
messages: Homotetramer.

224308:BSU 38630
gene id: 224308:BSU_38630
uniprot id: A0A6M4JQQ1
uniprot id: P94377
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the catalase family.
messages: Cytoplasm
messages: Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.

224308:BSU 02280
gene id: 224308:BSU_02280
uniprot id: O31453
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DedA family.
messages: Cell membrane

224308:BSU 39940
gene id: 224308:BSU_39940
uniprot id: P42111
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Increases the processivity of the PcrA helicase, but does not bind to DNA.
messages: Interacts with PcrA, Pdp, YclM, YkvL, YhcQ and YomL. The interaction with PcrA is not essential for cell viability or repair of UV-induced lesions.
messages: No PcrA-related phenotype.
messages: Secreted

224308:BSU 13030
gene id: 224308:BSU_13030
uniprot id: A0A6M4JRA5
uniprot id: P49851
names: Similarity
messages: Belongs to the acyl coenzyme A hydrolase family.

224308:BSU 28600
gene id: 224308:BSU_28600
uniprot id: A0A6M3ZI55
uniprot id: P94543
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 29490
gene id: 224308:BSU_29490
uniprot id: A0A6M3ZF80
uniprot id: P80864
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the peroxiredoxin family. Tpx subfamily.
messages: By superoxide.
messages: Homodimer.
messages: The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide. The disulfide is subsequently reduced by thioredoxin.
messages: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.

224308:BSU 22800
gene id: 224308:BSU_22800
uniprot id: A0A6M4JNA2
uniprot id: P35149
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: ATPase. Has a role at an early stage in the morphogenesis of the spore coat outer layers. Its ATP hydrolysis is required for proper assembly of the spore coat. Forms a basement layer around the outside surface of the forespore and self-assembles irreversibly into higher order structures by binding and hydrolyzing ATP thus creating a durable and stable platform upon which thereafter morphogenesis of the coat can take place. Required for proper localization of spore coat protein CotE and sporulation-specific proteins including SpoVM.
messages: ATPase. Has a role at an early stage in the morphogenesis of the spore coat.
messages: Cytoplasm
messages: Expressed soon after the formation of the asymmetric septum during sporulation. Expression commences about 2 hours after the onset of sporulation. Assembly around the developing forespore commences at the time of polar division and seems to continue after engulfment of the forespore is complete. Remains present throughout the late stages of morphogenesis and during this accumulation process preferentially collects on the mother cell side of the engulfed forespore, but eventually is deposited equally all around the forespore.
messages: Extreme C-terminal region (AA 487-492) is required for its proper localization into a spherical shell around the developing forespore. N-terminus (AA 1-64) is functionally important although largely dispensable for proper localization.
messages: Forespores lack a well-defined cortex, and the coat is present as swirls in the mother cell compartment of the sporangia rather than having been deposited around the forespore protoplasts. According to PubMed:18691972, unable to sporulate.
messages: Polymerizes to self-assemble into static filaments. ATP hydrolysis is required by every subunit for incorporation into the growing polymer by inducing a conformational change that drives polymerization of a nucleotide-free filament. Polymerization requires a critical concentration of the protein and only occurs after it is localized to the surface of the developing spore. Interacts (via extreme C-terminus Gly-486) with SpoVM (via Ile-6). Interacts (via full-length) with SpoVID (via C-terminus 499-575 AA). Interacts with SafA.
messages: Present in an increased level in yabG mutant spores.
messages: Seems to be cleaved by the YabG protease.

224308:BSU 32580
gene id: 224308:BSU_32580
uniprot id: A0A6M3ZJ74
uniprot id: O32154
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Probably part of the binding-protein-dependent transport system YurMNO. Probably responsible for the translocation of the substrate across the membrane.

224308:BSU 13940
gene id: 224308:BSU_13940
uniprot id: Q796K9
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 34920
gene id: 224308:BSU_34920
uniprot id: A0A6M3ZGY0
uniprot id: O34520
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATP phosphoribosyltransferase family. Short subfamily.
messages: Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity).
messages: Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.
messages: Cytoplasm
messages: Heteromultimer composed of HisG and HisZ subunits.
messages: Lacks the C-terminal regulatory region which is replaced by HisZ.

224308:BSU 24710
gene id: 224308:BSU_24710
uniprot id: A0A6M4JNP0
uniprot id: P25955
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ComGC family.
messages: Cell membrane
messages: Cell surface
messages: Homodimer.
messages: Processing of ComGC in competent cells requires ComC, while stabilization, possibly by formation of a disulfide bond, requires BdbC and BdbD.
messages: Required for transformation and DNA binding.

224308:BSU 13580
gene id: 224308:BSU_13580
uniprot id: O31665
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily.
messages: Disruption mutant grows more slowly than the wild type strain on methylthioribose (MTR) as the sulfur source.
messages: Involved in the methylthioribose (MTR) recycling pathway (PubMed:12022921, PubMed:24837359). Catalyzes the formation of methionine from 2-keto-4-methylthiobutyrate (KMTB) (PubMed:12670965, PubMed:24837359).

224308:BSU 04030
gene id: 224308:BSU_04030
uniprot id: P42961
names: Similarity
messages: Belongs to the thioester dehydratase family. FabZ subfamily.

224308:BSU 23020
gene id: 224308:BSU_23020
uniprot id: P50729
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the helicase family. RecQ subfamily.
messages: Cells lacking this gene have an increased sensitivity to DNA damaging agents, 100-fold decreased plasmid transformation and 4-fold reduced chromosomal DNA transformation. Sensitivity increases in the presence of mutated AddAB nuclease.
messages: Probable DNA helicase. Required in synaptic and/or post-synaptic stages of recombination. Probably has overlapping function with RecQ (AC O34748). It probably acts to help generate ss-DNA from ds-DNA breaks.

224308:BSU 31900
gene id: 224308:BSU_31900
uniprot id: A0A6M4JLR8
uniprot id: P71071
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the EsaB family.
messages: Cells lacking this gene are blocked in YukE secretion (PubMed:23861817, PubMed:24798022). They display an increased bacteriophage SPP1 resistance phenotype (PubMed:15576783).
messages: Required for YukE secretion. Probable component or regulator of the ESX/ESAT-6-like secretion system (BsEss).

224308:BSU 02850
gene id: 224308:BSU_02850
uniprot id: A0A6M4JCJ2
uniprot id: O34966
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 9 family.
messages: Cell membrane
messages: Cells lacking this gene grow slowly in zinc-deficient medium; this effect is suppressed upon addition of exogenous Zn(2+). Growth is slower yet in a double knockout with YciC. Disruption results in low transformability.
messages: Membrane raft
messages: Part of the ATP-binding cassette (ABC) transport system ZnuABC involved in zinc import (By similarity). Binds zinc with high affinity and specificity and delivers it to the membrane permease for translocation into the cytoplasm (By similarity). ZnuABC-mediated zinc transport is required for comF expression and competence development (PubMed:21813502, PubMed:9811636).
messages: Repressed by zinc via the metallo-regulatory protein Zur.
messages: The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA).

224308:BSU 22440
gene id: 224308:BSU_22440
uniprot id: A0A6M3ZD84
uniprot id: P0CI75
names: Caution
names: Function
names: Similarity
messages: Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.
messages: Belongs to the biotin--protein ligase family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 10840
gene id: 224308:BSU_10840
uniprot id: P37947
names: Function
messages: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease.

224308:BSU 35320
gene id: 224308:BSU_35320
uniprot id: P39740
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacillales FliT family.
messages: Homodimer.
messages: May act as an export chaperone for the filament capping protein FliD.
messages: cytosol

224308:BSU 01390
gene id: 224308:BSU_01390
uniprot id: A0A6M4JCJ0
uniprot id: P20458
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the IF-1 family.
messages: Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.
messages: Cytoplasm
messages: One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.

224308:BSU 19550
gene id: 224308:BSU_19550
uniprot id: P81102
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nitroreductase family.
messages: Cytoplasm
messages: Putative nitroreductase that may contribute to the degradation of aromatic compounds.
messages: Repressed by YodB. Strongly induced by stress due to exposure to catechol and less strongly induced after diamide or 2-methylhydroquinone (2-MHQ) stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 15450
gene id: 224308:BSU_15450
uniprot id: A0A6M4JIG4
uniprot id: Q45479
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase A8 family.
messages: Cell membrane
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: This protein specifically catalyzes the removal of signal peptides from prolipoproteins.

224308:BSU 15720
gene id: 224308:BSU_15720
uniprot id: A0A6M3ZB20
uniprot id: P94462
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the polypeptide deformylase family.
messages: Binds 1 Fe(2+) ion.
messages: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions (By similarity).
messages: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.

224308:BSU 07120
gene id: 224308:BSU_07120
uniprot id: A0A6M3Z8D5
uniprot id: P39129
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 29570
gene id: 224308:BSU_29570
uniprot id: A0A6M3ZF15
uniprot id: P04831
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the alpha/beta-type SASP family.
messages: SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.
messages: SASP are degraded in the first minutes of spore germination and provide amino acids for both new protein synthesis and metabolism.

224308:BSU 28750
gene id: 224308:BSU_28750
uniprot id: P94528
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Deletion of the gene does not affect growth on glucose and arabinose, but it has a negative effect on the ability of the mutant to grow on the alpha-1,5-arabinose oligomers alpha-1,5-arabinobiose, alpha-1,5-arabinotriose and alpha-1,5-arabinotetraose. AraE/araN double mutant is unable to grow in the presence of alpha-1,5-arabinobiose.
messages: Part of the ABC transporter complex AraNPQ involved in the uptake of arabinooligosaccharides. Transports alpha-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (PubMed:20693325). AraN captures the substrate and delivers it to the two transmembrane components (Probable).
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (AraP and AraQ) and a solute-binding protein (AraN).
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 13330
gene id: 224308:BSU_13330
uniprot id: O34763
names: Induction
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.

224308:BSU 29600
gene id: 224308:BSU_29600
uniprot id: A0A6M3ZIF0
uniprot id: O34545
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the branched chain amino acid transporter family.
messages: Branched-chain amino acid transport system which is involved in the uptake of isoleucine, valine and probably leucine (PubMed:25645558). Together with BcaP and BrnQ, plays an important role in the activation of CodY, a branched-chain amino acid-responsive transcriptional regulator that controls the expression of several dozen transcription units in B.subtilis (PubMed:25645558).
messages: Cell membrane
messages: Component of the transport system for branched-chain amino acids.
messages: Expression is under both negative and positive control by the transcriptional regulator CodY (PubMed:26473603). The negative control is direct and the positive control is indirect and mediated by another transcriptional regulator, ScoC (or Hpr), which, in turn, is repressed by CodY (PubMed:26473603). Thus, CodY and ScoC form a feed-forward regulatory loop in which CodY acts an indirect positive regulator of braB (PubMed:26473603). This feed-forward regulatory loop at the braB promoter is an arrangement in which two regulatory proteins repress the same target gene and one of the regulators represses expression of the other (PubMed:26473603).
messages: Membrane
messages: Mutant is still able to take up isoleucine, leucine and valine.

224308:BSU 06820
gene id: 224308:BSU_06820
uniprot id: O31507
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25050
gene id: 224308:BSU_25050
uniprot id: P54484
names: Subcellular Location Annotation
names: Subunit
messages: Found in a complex with F(1)F(0) ATP synthase and SpoIIIJ and YqjG.
messages: cell wall

224308:BSU 36390
gene id: 224308:BSU_36390
uniprot id: A0A6M4JQ01
uniprot id: P39753
names: Similarity
messages: Belongs to the flagella basal body rod proteins family.

224308:BSU 13420
gene id: 224308:BSU_13420
uniprot id: O34311
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells do not display any phenotype.
messages: Probable signaling protein whose physiological role is not yet known.

224308:BSU 28760
gene id: 224308:BSU_28760
uniprot id: A0A6M4JKW3
uniprot id: P94527
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycerol-1-phosphate dehydrogenase family.
messages: Binds 1 nickel ion per subunit.
messages: Binds 1 nickel ion per subunit. Is not active with other divalent metal cations such as Zn(2+), Cu(2+), Ca(2+), Mg(2+), Mn(2+) or Fe(2+).
messages: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species.
messages: Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species. Prefers NADH over NADPH as coenzyme. Is also able to catalyze the reverse reaction, i.e. the NAD(+)-dependent oxidation of G1P but not of G3P. Does not possess glycerol dehydrogenase activity.
messages: Cytoplasm
messages: Homodimer.
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 07400
gene id: 224308:BSU_07400
uniprot id: A0A6M3Z8U2
uniprot id: O06473
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Acts to efflux copper or a copper complex. It is possible that YfmO could contribute to copper resistance.
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Cotranscribed with yfmP.
messages: Membrane
messages: Repressed by YfmP.

224308:BSU 15330
gene id: 224308:BSU_15330
uniprot id: A0A6M4JG58
uniprot id: P19940
names: Activity Regulation
names: Developmental Stage
names: Domain
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Active only in the forespore (PubMed:18208527).
messages: Activity repressed by anti-sigma-G factor Gin (csfB) and Lon protease during the early stages of forespore development (PubMed:17921305). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G activity, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).
messages: Belongs to the sigma-70 factor family.
messages: Expressed and active 2 hours after sporulation starts (at protein level); stimulates its own transcription (PubMed:18208527).
messages: Interacts with anti-sigma-G factor Gin (csfB) (PubMed:18208527, PubMed:19497328).
messages: Recognition of anti-sigma-G factor Gin (csfB) occurs via the first 71 residues (PubMed:18208527).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (PubMed:18208527). This sigma factor is responsible for the expression of sporulation specific genes in the forespore (PubMed:18208527).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

224308:BSU 11080
gene id: 224308:BSU_11080
uniprot id: O06752
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27860
gene id: 224308:BSU_27860
uniprot id: A0A6M4JJZ2
uniprot id: P39666
names: Caution
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the NadC/ModD family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Hexamer formed by 3 homodimers.
messages: Involved in the catabolism of quinolinic acid (QA).
messages: It is uncertain whether Met-1, Leu-59 or Met-74 is the initiator.

224308:BSU 39450
gene id: 224308:BSU_39450
uniprot id: P54957
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EutH family.
messages: Cell membrane

224308:BSU 27480
gene id: 224308:BSU_27480
uniprot id: A0A6M4JLQ4
uniprot id: O34481
names: Function
names: Similarity
messages: Belongs to the RecD family. RecD-like subfamily.
messages: DNA-dependent ATPase and ATP-dependent 5'-3' DNA helicase. Has no activity on blunt DNA or DNA with 3'-overhangs, requires at least 10 bases of 5'-ssDNA for helicase activity.

224308:BSU 00760
gene id: 224308:BSU_00760
uniprot id: A0A6M3ZIC8
uniprot id: P28821
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
messages: Homodimer.
messages: Involved in the biosynthesis of p-aminobenzoate (PABA), a precursor of tetrahydrofolate. Converts 4-amino-4-deoxychorismate into 4-aminobenzoate (PABA) and pyruvate (By similarity).
messages: Requires p-aminobenzoic acid for growth.

224308:BSU 16710
gene id: 224308:BSU_16710
uniprot id: A0A6M4JM03
uniprot id: Q04805
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M16 family.
messages: Binds 1 zinc ion per subunit.

224308:BSU 33570
gene id: 224308:BSU_33570
uniprot id: A0A6M3ZGD3
uniprot id: O32227
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 25100
gene id: 224308:BSU_25100
uniprot id: A0A6M4JME9
uniprot id: P54479
names: Activity Regulation
names: Cofactor
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acts as a negative controlling element, employing Zn(2+) as a cofactor to bind the operator of the repressed genes. Required for the zinc-specific repression of two operons implicated in zinc uptake, yciC and ycdHIyceA. Also represses the expression of rpmE2, the gene for ribosomal protein L31B, which is expressed only after the end of exponential growth.
messages: Belongs to the Fur family.
messages: Binds 1 Mn(2+) or Fe(2+) ion per subunit.
messages: Binds 1 zinc ion per subunit.
messages: Contains 3 zinc-binding sites. Site 1 has a structural role and site 2 is the Zn(2+) sensing site. Site 3 residues do not bind metal ions with a physiologically relevant affinity, but contribute to dimer stability.
messages: Cytoplasm
messages: Homodimer.
messages: Sequentially activated from an inactive dimer (Zur(2):Zn(2)) to a partially active asymmetric dimer (Zur(2):Zn(3)), and finally to the fully zinc-loaded active form (Zur(2):Zn(4)). Binds a maximum of 4 Zn(2+) ions per dimer.

224308:BSU 03460
gene id: 224308:BSU_03460
uniprot id: A0A6M3Z7A7
uniprot id: P42405
names: Function
names: Induction
names: Similarity
messages: Belongs to the HPS/KGPDC family. HPS subfamily.
messages: By formaldehyde, under the control of HxlR. Not induced by methanol, formate, or methylamine.
messages: Catalyzes the condensation of ribulose 5-phosphate with formaldehyde to form 3-hexulose 6-phosphate. Together with HxlB, may act as a formaldehyde detoxification system.

224308:BSU 09530
gene id: 224308:BSU_09530
uniprot id: P80874
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Aldo-keto reductase (AKR) that displays broad substrate specificity in vitro. Is able to reduce the standard AKR substrates DL-glyceraldehyde, D-erythrose, methylglyoxal, p-nitrobenzaldehyde, benzaldehyde and butyraldehyde, in the presence of NADPH. Cannot use NADH as a cosubstrate. Does not act on glucose, 2-pyridine carboxyaldehyde, fructose and xylose. The physiological function of this enzyme is not clear. May play a role in bacterial stress response and/or in detoxification of reactive aldehydes.
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 11 subfamily.
messages: Is under the control of the sigma-B transcription factor. Is induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Monomer.

224308:BSU 33490
gene id: 224308:BSU_33490
uniprot id: A0A6M3ZGN3
uniprot id: O32219
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Arrested in competence development and sporulation.
messages: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
messages: By heat-shock, ethanol stress, zinc, cobalt and cadmium.
messages: Cell membrane
messages: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper.
messages: Expression increases rapidly at 5 hours and peaks at 7 hours after onset of sporulation.

224308:BSU 36850
gene id: 224308:BSU_36850
uniprot id: A0A6M3ZK01
uniprot id: P37814
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase B chain family.
messages: Cell membrane
messages: Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).
messages: F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Membrane

224308:BSU 14780
gene id: 224308:BSU_14780
uniprot id: O07632
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30580
gene id: 224308:BSU_30580
uniprot id: O34493
names: Similarity
messages: Belongs to the peptidase S9B family.

224308:BSU 08740
gene id: 224308:BSU_08740
uniprot id: A0A6M3Z8R2
uniprot id: Q7WY74
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0295 family.
messages: Cell membrane

224308:BSU 15140
gene id: 224308:BSU_15140
uniprot id: A0A6M4JID5
uniprot id: Q07876
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyltransferase superfamily. RsmH family.
messages: Cytoplasm
messages: No visible phenotype.
messages: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.
messages: Transcribed at a low level in exponential phase, it may be further induced in stationary phase. Part of the mraZ-rsmH-ftsL-pbpB operon.

224308:BSU 31860
gene id: 224308:BSU_31860
uniprot id: A0A6M4JP02
uniprot id: O32101
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EsaA family.
messages: Cell membrane
messages: Cells lacking this gene are blocked in YukE secretion.
messages: Membrane
messages: Required for YukE secretion. Probable component or regulator of the ESX/ESAT-6-like secretion system (BsEss) (PubMed:23861817, PubMed:24798022). Bacteriophage SPP1 receptor. Essential for the irreversible adsorption of the bacteriophage (PubMed:15576783).

224308:BSU 04740
gene id: 224308:BSU_04740
uniprot id: P17906
names: Function
messages: Negative regulator of sigma-B activity. Dephosphorylates RsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels.

224308:BSU 21330
gene id: 224308:BSU_21330
uniprot id: O31974
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39390
gene id: 224308:BSU_39390
uniprot id: A0A6M3ZHX3
uniprot id: P42087
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 39100
gene id: 224308:BSU_39100
uniprot id: A0A6M4JQP0
uniprot id: P42306
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 18220
gene id: 224308:BSU_18220
uniprot id: A0A6M4JH76
uniprot id: O34893
names: Similarity
messages: Belongs to the enoyl-CoA hydratase/isomerase family.

224308:BSU 30230
gene id: 224308:BSU_30230
uniprot id: A0A6M4JMC4
uniprot id: P53555
names: Activity Regulation
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.
messages: Catalyzes the transfer of the alpha-amino group from L-lysine to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). B.subtilis is the only bacterium known to utilize L-lysine as an amino donor in the biosynthesis of DAPA.
messages: Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
messages: Cytoplasm
messages: Homodimer.
messages: Inhibited by 7-keto-8-aminopelargonic acid at concentrations above 80 uM.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 09670
gene id: 224308:BSU_09670
uniprot id: A0A6M3Z924
uniprot id: O07597
names: Function
names: Similarity
names: Subunit
messages: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction.
messages: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction. The second half-reaction is the reverse of the first, transferring the amino group from the pyridoxamine to a second alpha-keto acid to form the product D-amino acid via a ping-pong mechanism. This is an important process in the formation of D-alanine and D-glutamate, which are essential bacterial cell wall components (By similarity).
messages: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
messages: Homodimer.

224308:BSU 07570
gene id: 224308:BSU_07570
uniprot id: O34726
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Activated by the two-component regulatory system MalK/MalR in response to malate. The regulator MalR binds to the promoter region of yflS.
messages: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.
messages: Cell membrane
messages: Might be a malate transporter.

224308:BSU 12940
gene id: 224308:BSU_12940
uniprot id: A0A6M3Z9Q6
uniprot id: P26904
names: Developmental Stage
names: Function
names: Induction
names: Sequence Caution
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Expressed early during sporulation.
messages: Membrane
messages: Nutrient deficiency conditions, which also induce sporulation.
messages: Probably part of the ABC transporter DppBCDE involved in dipeptide transport (Probable). Responsible for the translocation of the substrate across the membrane (Probable).
messages: Sequencing errors.

224308:BSU 24100
gene id: 224308:BSU_24100
uniprot id: P54529
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00600
gene id: 224308:BSU_00600
uniprot id: P37558
names: Developmental Stage
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expressed in the mother cell compartment at the second hour of sporulation.
messages: Expression is sigma E-dependent and negatively regulated by spo0A.
messages: Forespore outer membrane
messages: Required for sporulation.

224308:BSU 38650
gene id: 224308:BSU_38650
uniprot id: A0A6M4JQK8
uniprot id: P94375
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is sigma Y-dependent. Induced upon nitrogen starvation.
messages: Membrane

224308:BSU 22890
gene id: 224308:BSU_22890
uniprot id: A3F447
uniprot id: P38493
names: Disruption Phenotype
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytidylate kinase family. Type 1 subfamily.
messages: Cytoplasm
messages: Essential, it cannot be disrupted.
messages: Transcribed during sporulation. May not be transcribed with the downstream gene (a ribosomal protein bS1 homolog), there is a probable transcription terminator between the 2 genes.

224308:BSU 38680
gene id: 224308:BSU_38680
uniprot id: P94372
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is sigma Y-dependent. Induced upon nitrogen starvation.
messages: Together with YxlE, is important for negative regulation of sigma Y activity, being the major negative regulator.

224308:BSU 39250
gene id: 224308:BSU_39250
uniprot id: A0A6M3ZHM1
uniprot id: P42297
names: Similarity
messages: Belongs to the universal stress protein A family.

224308:BSU 30960
gene id: 224308:BSU_30960
uniprot id: A0A6M3ZFW3
uniprot id: P39124
names: Function
names: Induction
names: Similarity
messages: Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.
messages: Expressed exclusively on media containing carbon sources that allow efficient sporulation.
messages: Required for the synthesis of glycogen.

224308:BSU 29805
gene id: 224308:BSU_29805
uniprot id: A0A6M4JMP9
uniprot id: C0SP86
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsK/SpoIIIE/SftA family.
messages: Cytoplasm
messages: Delay in chromosome segregation and an increase in the sensitivity to agents that induce DNA damage. They divide over unsegregated chromosomes with increased frequencies. A cell filamentation phenotype can also be seen, when associated with a noc deletion.
messages: Homohexamer.
messages: Required for the accurate completion of chromosome partitioning, in part by promoting efficient resolution of chromosome dimers, before the formation of the division septum. Binds to DNA in a non-specific manner. Shows ATPase activity. Not required for cytokinesis.
messages: StfA and SpoIIIE are not functionally redundant. They probably play distinct roles during growth and sporulation.

224308:BSU 39020
gene id: 224308:BSU_39020
uniprot id: A0A6M4JQM9
uniprot id: P42312
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.
messages: Cell membrane
messages: Expression is regulated by the XptR regulon. Negatively regulated by hypoxanthine and guanine.
messages: Involved in the uptake of the purine ribonucleosides inosine and guanosine.
messages: Membrane

224308:BSU 11850
gene id: 224308:BSU_11850
uniprot id: A0A6M4JF48
uniprot id: O31629
names: Similarity
names: Subunit
messages: Belongs to the 2H phosphoesterase superfamily. YjcG family.
messages: Homodimer.

224308:BSU 35330
gene id: 224308:BSU_35330
uniprot id: A0A6M4JPP3
uniprot id: P39739
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FliS family.
messages: Essential for filament assembly (PubMed:23144244). May act as a facilitator of flagellin (hag) secretion. Antagonizes translational repressor CsrA indirectly (PubMed:23144244).
messages: Homodimer (PubMed:23144244, PubMed:16021622). Interacts directly with flagellin (hag), forms a 3-way complex of Hag, FliS and FliW in which Flis and FliW do not directly interact (PubMed:23144244).
messages: Severe defect in motility on agar plates, forms multiple short filament stubs on the cell surface, decreased expression of flagellin (hag), 30-fold decrease in Hag secretion. All phenotypes are partially suppressed by deletion of csrA, which increases translation of Hag (PubMed:23144244).
messages: cytosol

224308:BSU 06950
gene id: 224308:BSU_06950
uniprot id: A0A6M3Z8M0
uniprot id: O31516
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YesM/YesN. Probably activates YesN by phosphorylation.
messages: Membrane

224308:BSU 01450
gene id: 224308:BSU_01450
uniprot id: A0A6M4JEU5
uniprot id: P40735
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.
messages: Belongs to the ABC transporter superfamily.
messages: Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.
messages: Cell membrane
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).
messages: Membrane

224308:BSU 28040
gene id: 224308:BSU_28040
uniprot id: A0A6M3ZF28
uniprot id: Q02170
names: Induction
names: Similarity
messages: Belongs to the UPF0758 family.
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 11450
gene id: 224308:BSU_11450
uniprot id: A0A6M3Z9U3
uniprot id: P24139
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 10680
gene id: 224308:BSU_10680
uniprot id: O06717
names: Developmental Stage
names: Function
names: Induction
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 08550
gene id: 224308:BSU_08550
uniprot id: A0A6M4JGP7
uniprot id: Q7WY75
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspK family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination. Expression is sigma G-dependent.
messages: Spore core

224308:BSU 20710
gene id: 224308:BSU_20710
uniprot id: O34509
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 08350
gene id: 224308:BSU_08350
uniprot id: Q79F14
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: An esterase which preferentially hydrolyzes triacylglyceride substrates with short chain fatty acids (C3-C10) with the maximum activity towards tricaprylin (C8:0); essentially inactive on C18:1 or C18:4 substrates. Active against p-nitrophenylesters with fatty acid chain lengths from C6 to C18.
messages: Belongs to the AB hydrolase superfamily.
messages: Induced in rich but not minimal media with glucose as carbon source (at protein level). Induced by growth on n-hexadecane and tributyrin.
messages: Secreted

224308:BSU 11740
gene id: 224308:BSU_11740
uniprot id: Q08312
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Disulfide cross-linked either to itself or to CotY.
messages: Spore coat
messages: To B.subtilis CotY.

224308:BSU 27680
gene id: 224308:BSU_27680
uniprot id: A0A6M4JLS2
uniprot id: O32050
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0702 family.
messages: Cell membrane
messages: Membrane

224308:BSU 05240
gene id: 224308:BSU_05240
uniprot id: A0A6M4JDN4
uniprot id: P96669
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 21500
gene id: 224308:BSU_21500
uniprot id: O31990
names: Cofactor
names: Similarity
messages: Belongs to the DNA polymerase type-Y family.
messages: Binds 2 magnesium ions per subunit.

224308:BSU 36030
gene id: 224308:BSU_36030
uniprot id: A0A6M3ZH22
uniprot id: O05224
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ArsB family.
messages: Cell membrane
messages: Membrane

224308:BSU 33290
gene id: 224308:BSU_33290
uniprot id: P49938
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Iron (Fe3+)-hydroxamate importer (TC 3.A.1.14.7) family.
messages: Cell membrane
messages: Part of the ABC transporter complex FhuBGCD involved in iron(3+)-hydroxamate import. Responsible for energy coupling to the transport system (By similarity).
messages: The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB and FhuG) and a solute-binding protein (FhuD or YxeB).

224308:BSU 11480
gene id: 224308:BSU_11480
uniprot id: A0A6M3ZI90
uniprot id: O31600
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Drug:H(+) antiporter-3 (DHA3) (TC 2.A.1.21) family.
messages: Cell membrane

224308:BSU 08480
gene id: 224308:BSU_08480
uniprot id: A0A6M4JEG2
uniprot id: O31571
names: Cofactor
names: Similarity
messages: Belongs to the nitroreductase family.
messages: Binds 1 FMN per subunit.

224308:BSU 27670
gene id: 224308:BSU_27670
uniprot id: A0A6M4JKH4
uniprot id: Q00758
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide synthase family.
messages: Cell membrane
messages: Involved, directly or indirectly, in spore cortex biosynthesis. Affects only indirectly the expression of late sporulation genes.
messages: Membrane
messages: SpoVB transcription takes place during the second hour of sporulation. It may be transcribed mainly, if not only, in the mother cell. Indeed, it is required only in the mother cell.

224308:BSU 10560
gene id: 224308:BSU_10560
uniprot id: O07567
names: Function
messages: Positively regulates the ntdABC operon and negatively regulates its own transcription. Binds to NTD to induce ntdABC transcription.

224308:BSU 19140
gene id: 224308:BSU_19140
uniprot id: O31845
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 17430
gene id: 224308:BSU_17430
uniprot id: A0A6M3ZBE4
uniprot id: P94478
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.
messages: Cytoplasm
messages: GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.
messages: Monomer. Associates with the 50S ribosomal subunit.
messages: No visible phenotype.

224308:BSU 01280
gene id: 224308:BSU_01280
uniprot id: A0A6M4JCI1
uniprot id: P12877
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL5 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome (By similarity). Interacts with VmlR (PubMed:30126986).
messages: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.
messages: This is 1 of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.
messages: This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.

224308:BSU 23180
gene id: 224308:BSU_23180
uniprot id: P35157
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in spore core dehydration; might be involved in the transport of something into or out of the forespore or could be required for some modification of the cortex peptidoglycan structure.

224308:BSU 22610
gene id: 224308:BSU_22610
uniprot id: A3F3C4
uniprot id: P20692
names: Similarity
messages: Belongs to the prephenate/arogenate dehydrogenase family.

224308:BSU 23500
gene id: 224308:BSU_23500
uniprot id: A0A6M4JJ03
uniprot id: P46353
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phosphopentomutase family.
messages: Binds 1 or 2 manganese ions.
messages: Cytoplasm
messages: Phosphotransfer between the C1 and C5 carbon atoms of pentose.

224308:BSU 18570
gene id: 224308:BSU_18570
uniprot id: C0SP82
names: Cofactor
names: Similarity
messages: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.

224308:BSU 34880
gene id: 224308:BSU_34880
uniprot id: A0A6M4JPH9
uniprot id: O35006
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the HisA/HisF family.
messages: Cytoplasm

224308:BSU 36550
gene id: 224308:BSU_36550
uniprot id: P71044
names: Developmental Stage
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Forespore membrane
messages: Interacts with SpoIIIAH and SpoIIE.
messages: Involved in forespore engulfment and required for anchoring membrane proteins on the forespore side of the septal membrane. Forms a channel with SpoIIIAH that is open on the forespore end and closed (or gated) on the mother cell end. This allows sigma-E-directed gene expression in the mother-cell compartment of the sporangium to trigger the activation of sigma-G forespore-specific gene expression by a pathway of intercellular signaling.
messages: Specifically expressed in the forespore under the control of the sigma-K factor.

224308:BSU 31490
gene id: 224308:BSU_31490
uniprot id: P40750
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth (PubMed:9851991).
messages: Expression increases during vegetative growth and decreases soon after entry into sporulation.
messages: In the C-terminal section; belongs to the transpeptidase family.
messages: In the N-terminal section; belongs to the glycosyltransferase 51 family.
messages: It is uncertain whether Met-1 or Met-3 is the initiator.
messages: No visible phenotype (PubMed:7961491). Double pbpA-pbpD deletions spores have greatly decreased spore outgrowth and peptidoglycan synthesis (PubMed:9851991).
messages: The N-terminus is blocked.

224308:BSU 35380
gene id: 224308:BSU_35380
uniprot id: A0A6M4JPP0
uniprot id: P96503
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin (hag), which is implicated in polymerization, and participates in the assembly of the flagellum (PubMed:16936039). An antagonist to translational regulator CsrA, it binds CsrA at an allosteric site and non-competitively inhibits CsrA binding to hag RNA (PubMed:27516547). Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis. Flagellin binds to assembly factor FliW, freeing translation regulator CsrA to repress translation of the flagellin mRNA. When the flagellar hook is assembled flagellin is secreted, depleting intracellular flagellin, which frees FliW to interact with CsrA and inhibits CsrA binding to mRNA. This derepresses flagellin translation and provides protein for flagellar assembly. Once the flagellar filament is completed cytoplasmic flagellin levels rise and CsrA translation repression of flagellin reinitiates (PubMed:21895793). Binds to CsrA and displaces it from hag mRNA (PubMed:21895793, PubMed:27516547). Binds to hag mRNA itself, but only at much higher concentrations than those required to displace CsrA (PubMed:21895793, PubMed:27516547).
messages: Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin and participates in the assembly of the flagellum.
messages: Belongs to the FliW family.
messages: Cytoplasm
messages: Greatly reduced swarming motility, less flagellin (PubMed:16936039, PubMed:21895793). Fewer, shorter flagella assemble (PubMed:21895793). Loss of motility and flagellar assembly are suppressed by deletion of csrA (PubMed:21895793). Decreased expression of flagellin (Hag) which is suppressed by deletion of csrA (PubMed:23144244).
messages: Interacts with flagellin in a 1:1 complex (PubMed:16936039, PubMed:21895793). Two molecules interact with each CsrA dimer; cannot interact with both flagellin and CsrA simultaneously (PubMed:21895793, PubMed:27516547). Has a higher affinity for CsrA than for flagellin (PubMed:21895793). Interacts directly with flagellin (hag), forms a 3-way complex of Hag, FliS and FliW in which Flis and FliW do not directly interact (PubMed:23144244). Interaction with Hag may occur via the C-terminus of Hag (PubMed:16936039).
messages: Interacts with translational regulator CsrA and flagellin(s).
messages: Part of the SigD-controlled yvyF-csrA operon and the SigA-controlled fliW-csrA operon (PubMed:17555441).

224308:BSU 33420
gene id: 224308:BSU_33420
uniprot id: A0A6M3ZFZ3
uniprot id: O32212
names: Caution
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
messages: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. Nhak (TC 2.A.36.3.2) subfamily.
messages: Cell membrane
messages: Constitutively expressed, with higher levels at stationary phase.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons.
messages: Transporter involved in the efflux of sodium, potassium, lithium and rubidium.

224308:BSU 00870
gene id: 224308:BSU_00870
uniprot id: A0A6M4JEY3
uniprot id: P37572
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RecA family. RadA subfamily.
messages: DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.
messages: General sensitivity to DNA damaging agents. Increased sensitivity to methyl methanesulfonate (MMS), decreased survival after UV irradiation in transition and stationary phase, decrease in transformation with chromosomal DNA requiring homologous recombination (PubMed:9141693, PubMed:11810266). Increased sensitivity to mitomycin C, H(2)O(2), and nalidixic acid; a further disA deletion suppresses H(2)O(2) sensitivity but has no effect on MMS sensitivity, suggesting radA and disA might work in the same DNA repair pathway (PubMed:25616256). Partially suppresses DNA damaging agent sensitivity of recU deletions (PubMed:11810266). Suppresses a chromosome segregation defect in ruvA and recD mutants cells, but not in recU mutant cells (PubMed:15317759).
messages: Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.
messages: Interacts with DisA (PubMed:23760274).
messages: Plays a role in DNA repair (PubMed:9141693, PubMed:11810266). Might stabilize or process Holliday junction intermediates (PubMed:15317759). May work with DisA following methyl methanesulfonate (MMS) but not H(2)O(2) damage; DisA is a DNA integrity scanning protein with c-di-AMP synthase activity.
messages: Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.
messages: The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.

224308:BSU 37170
gene id: 224308:BSU_37170
uniprot id: A0A6M3ZH84
uniprot id: P45867
names: Function
names: Induction
names: Similarity
messages: Belongs to the acyl-CoA dehydrogenase family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 18910
gene id: 224308:BSU_18910
uniprot id: O34930
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rap family.
messages: Cytoplasm
messages: Deletion of the gene has no detectable effect on expression of the srfA operon.
messages: Inhibited by PhrK, which prevents RapK-ComA interaction.
messages: Involved in the regulation of genetic competence development (PubMed:16816200). Inhibits the activity of ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:16816200). Likely affects the activity of additional regulators, in particular Spo0A (PubMed:16816200).

224308:BSU 32180
gene id: 224308:BSU_32180
uniprot id: A0A6M3ZKM1
uniprot id: O32115
names: Caution
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.
messages: Cell membrane
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane

224308:BSU 14770
gene id: 224308:BSU_14770
uniprot id: A0A6M4JJN2
uniprot id: O07631
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: 3-fold induction by growth at 15 degrees Celsius.
messages: A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.
messages: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.
messages: Cytoplasm
messages: Monomer.
messages: No visible growth phenotype at 15 degrees Celsius.

224308:BSU 11560
gene id: 224308:BSU_11560
uniprot id: A0A6M4JHF0
uniprot id: O31607
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the truncated hemoglobin family. Group II subfamily.
messages: Binds 1 heme group per subunit.
messages: Hemoglobin-like protein that exhibits a low peroxidase activity. Its very high oxygen affinity may rule out the possibility that it is involved in oxygen transport.
messages: Monomer.

224308:BSU 35290
gene id: 224308:BSU_35290
uniprot id: A0A6M4JPM9
uniprot id: P28367
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the prokaryotic/mitochondrial release factor family.
messages: Cytoplasm
messages: Methylated by PrmC. Methylation increases the termination efficiency of RF2 (By similarity).
messages: Methylated by PrmC. Methylation increases the termination efficiency of RF2.
messages: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.
messages: The gene for this protein contains a UGA in-frame termination codon after Leu-24; a naturally occurring frameshift enables complete translation of RF-2. This provides a mechanism for the protein to regulate its own production.

224308:BSU 34010
gene id: 224308:BSU_34010
uniprot id: A0A6M4JPC2
uniprot id: O32257
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Induced in response to leucine limitation. Regulated via the T box transcriptional antitermination system.
messages: May participate in leucine metabolism. May transport leucine or a compound related to leucine metabolism.
messages: Membrane
messages: No growth phenotype is observed for the knockout strain.

224308:BSU 06270
gene id: 224308:BSU_06270
uniprot id: O34759
names: Subunit
messages: Interacts with the RNA polymerase core.

224308:BSU 33050
gene id: 224308:BSU_33050
uniprot id: A0A6M3ZG95
uniprot id: P07868
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Forms a complex at the inner spore membrane which acts as a receptor for L-alanine, thus is involved in the stimulation of germination in response to alanine. Can stimulate germination in the absence of GerD and GerK gene products (fructose and glucose receptors, respectively), but the response is improved in their presence.
messages: Membrane

224308:BSU 10200
gene id: 224308:BSU_10200
uniprot id: A0A6M3Z9M5
uniprot id: O07603
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M42 family.
messages: Binds 2 divalent metal cations per subunit.

224308:BSU 33060
gene id: 224308:BSU_33060
uniprot id: A0A6M4JP29
uniprot id: P07869
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Involved in the germinative response to L-alanine. Could be an amino acid transporter. Forms a complex at the inner spore membrane which acts as a receptor for L-alanine, thus is involved in the stimulation of germination in response to alanine. Can stimulate germination in the absence of gerD and gerK gene products (fructose and glucose receptors, respectively), but the response is improved in their presence.
messages: Membrane

224308:BSU 03390
gene id: 224308:BSU_03390
uniprot id: A0A6M4JEU1
uniprot id: P42401
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 27840
gene id: 224308:BSU_27840
uniprot id: O32062
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: In wild-type cells, during the early stages of sporulation, a product of 43 kDa (the predicted size of SafA) was detected. However, in later stages, a 30-kDa species was the major form of SafA detected. The N-terminus sequence of the 30-kDa product starts at Met-164. It is not known if the smaller form results from proteolysis or from translation initiated at Met-164.
messages: Probably involved in the assembly of some coat protein components implicated in both lysozyme resistance and germination. Could be required for the assembly of CotG. Associates with SpoIVD during the early stage of coat assembly.
messages: Several coat proteins are extracted in reduced amounts from SafA mutant spores, one of which is CotG. SafA mutant spores have abnormal coat layers and have lost their resistance to lysozyme. Their response to L-alanine suggest that they have a defect at a late stage of spore germination. Their response to AGFK also suggests that they have an additional defect at a early stage of spore germination. The mutation of SafA has no effect on vegetative growth and spore resistance to heat and chloroform.
messages: Spore cortex
messages: Transcribed by SigE at time T2 of sporulation.

224308:BSU 37360
gene id: 224308:BSU_37360
uniprot id: Q7WY57
names: Induction
names: Miscellaneous
messages: In stationary phase of anaerobic cultures.
messages: Overlaps sboA.

224308:BSU 06510
gene id: 224308:BSU_06510
uniprot id: A0A6M4JDZ6
uniprot id: P12040
names: Function
names: Similarity
messages: Belongs to the GART family.
messages: Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.

224308:BSU 06310
gene id: 224308:BSU_06310
uniprot id: A0A6M3Z802
uniprot id: P46349
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: A transposon insertion in this gene eliminates the uptake of GABA and severely inhibits the utilization of GABA as a nitrogen source (PubMed:8951816). Triple deletion of gabP, putP and opuE confers resistance to the proline analog 3,4-dehydro-DL-proline (DHP), abolishes GABA utilization and prevents use of proline as a nitrogen source (PubMed:24142252).
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family.
messages: Cell membrane
messages: Membrane
messages: Positively regulated by TnrA under conditions of nitrogen limitation (PubMed:8951816). Expression is not significantly induced by GABA (PubMed:8951816).
messages: The ligand-recognition profile of the B.subtilis GabP was found to differ substantially from that of the highly homologous E.coli GabP. B.subtilis GabP exhibits more stringent requirements than E.coli GabP for substrate recognition and translocation.
messages: Transporter for gamma-aminobutyrate (GABA) (PubMed:8951816, PubMed:9677314, PubMed:24142252). Can also transport beta-alanine (PubMed:9677314). Can translocate several open-chain GABA analogs (3-aminobutyrate, 3-aminopropanoate, cis-4-aminobutenoate) across the membrane via counterflow against GABA, but cannot transport muscimol (PubMed:9677314). Functions also as a low-affinity proline importer (PubMed:24142252).

224308:BSU 39650
gene id: 224308:BSU_39650
uniprot id: P42422
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Probable member of the two-component regulatory system YxdK/YxdJ. May activate YxdJ in response to the antibacterial protein LL-37.

224308:BSU 29020
gene id: 224308:BSU_29020
uniprot id: A0A6M4JMC6
uniprot id: O34425
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
messages: Cells lacking this gene are unable to grow in medium containing asparagine as a sole carbon source but presents the same growth rate as the wild-type in glucose-containing medium.
messages: Cytoplasm
messages: Homotetramer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: In response to oxidative stress, the active site Cys likely reacts with bacillithiol (BSH) to form mixed disulfides to protect the Cys residue against overoxidation. S-bacillithiolation presumably leads to loss of catalytic activity. Debacillithiolation by monothiol bacilliredoxin BrxC restores the activity.
messages: Involved in the gluconeogenesis. Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NADP. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NADP to NADPH. The reduced NADPH is then exchanged with the second NADP, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

224308:BSU 04190
gene id: 224308:BSU_04190
uniprot id: P80873
names: Induction
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 01300
gene id: 224308:BSU_01300
uniprot id: A0A6M4JHT7
uniprot id: P12879
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS8 family.
messages: One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts proteins S5 and S12 (By similarity).
messages: Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.

224308:BSU 27170
gene id: 224308:BSU_27170
uniprot id: O08335
names: Function
messages: Negatively regulates the transcription of the fatR-cypB operon. Is displaced from its operator by a range of fatty acids such as oleate, linoleate and phytanate, thereby allowing transcription of the fatR-cypB operon.

224308:BSU 35510
gene id: 224308:BSU_35510
uniprot id: P32437
names: Similarity
messages: Belongs to the IMPACT family.

224308:BSU 06360
gene id: 224308:BSU_06360
uniprot id: A0A6M4JFL8
uniprot id: P29727
names: Function
names: Subunit
messages: Catalyzes the synthesis of GMP from XMP.
messages: Homodimer.

224308:BSU 03100
gene id: 224308:BSU_03100
uniprot id: P94382
names: Similarity
messages: Belongs to the DcsA family.

224308:BSU 19110
gene id: 224308:BSU_19110
uniprot id: P94497
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Transcription is activated at hour 4 of sporulation, requires sigma-K and is repressed by GerE. Maximal expression depends on SpoIIID.

224308:BSU 05170
gene id: 224308:BSU_05170
uniprot id: P96662
names: Subcellular Location Annotation
messages: Cytoplasm

224308:BSU 38260
gene id: 224308:BSU_38260
uniprot id: A0A6M3ZK84
uniprot id: P39597
names: Biotechnology
names: Cofactor
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DyP-type peroxidase family.
messages: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently per subunit.
messages: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently.
messages: Catalyzes the peroxide-dependent oxidation of various industrial dyes contributing to their detoxification and thus can be used for the bioremediation of dye-contaminated wastewater.
messages: Cell membrane
messages: Component of the iron transporter efeUOB/M complex composed of EfeU, EfeM and EfeB; EfeU is essential for the complex formation.
messages: Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
messages: Involved in the recovery of exogenous heme iron (By similarity). Extracts iron from heme while preserving the protoporphyrin ring intact (By similarity). Part of the iron transporter system efeUOB/M involved in iron import (PubMed:16672620, PubMed:23764491). Catalyzes the peroxide-mediated oxidation of Fe(2+) into Fe(3+); EfeM binds Fe(3+) and delivers it to the cell membrane permease EfeU (PubMed:23764491).
messages: Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the protoporphyrin ring intact.
messages: Secreted
messages: Severe reduction in Fe(2+) uptake and in cell growth under anaerobic conditions but not in aerobic and microaerobic conditions.

224308:BSU 04620
gene id: 224308:BSU_04620
uniprot id: A0A6M4JFL5
uniprot id: P96618
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the P-Pant transferase superfamily. AcpS family.
messages: Belongs to the P-Pant transferase superfamily. Gsp/Sfp/HetI/AcpT family.
messages: Cytoplasm
messages: Homotrimer.
messages: Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.
messages: Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of fatty acid acyl-carrier-protein ACP. Also modifies the D-alanyl carrier protein but fails to recognize PCP and AcpK, an acyl carrier protein of secondary metabolism.

224308:BSU 07980
gene id: 224308:BSU_07980
uniprot id: O34928
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the polysaccharide deacetylase family.
messages: Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in glycan strands of peptidoglycan, leading to the formation of muramic delta-lactam residues in spore cortex, after transpeptidation of deacetylated muramic acid residues. PdaA probably carries out both deacetylation and lactam ring formation and requires the product of CwlD activity on peptidoglycan as a substrate. Is required for germination. Cannot use chitin oligomer (hexa-N-acetylchitohexaose) as a substrate.
messages: CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.
messages: Expressed in the forespore, then exported into the developing cortex.
messages: Expression is sigma G-dependent.
messages: Spores have no muramic delta-lactam structure in the cortex and cannot germinate. Mutant spore peptidoglycan possesses many MurNAc residues lacking peptide side chains.

224308:BSU 22780
gene id: 224308:BSU_22780
uniprot id: A3F3E1
uniprot id: P19465
names: Activity Regulation
names: Similarity
names: Subunit
messages: Belongs to the GTP cyclohydrolase I family.
messages: Homopolymer.
messages: K(+) ions moderately increases the Vmax, whereas UTP and Ca(2+) and Mg(2+) ions drastically increase the Km for GTP.
messages: Toroid-shaped homodecamer, composed of two pentamers of five dimers.

224308:BSU 36930
gene id: 224308:BSU_36930
uniprot id: A0A6M3ZGZ2
uniprot id: P39155
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
messages: Catalyzes the specific dephosphorylation of phosphoarginine residues in a large number of proteins. Counteracts the protein arginine kinase McsB in vivo. Can dephosphorylate CtsR-P; thus, can restore the DNA-binding ability of the CtsR repressor by reversing the McsB-mediated phosphorylation. Is the only active pArg phosphatase present in B.subtilis. Exhibits almost no activity against pSer, pThr, or pTyr peptides. Appears to play a role in B.subtilis stress resistance. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity.
messages: Cellular protein arginine phosphorylation is only detectable in a ywlE mutant and not in the wild-type strain, and global gene expression is significantly impacted in the mutant strain (PubMed:22517742, PubMed:24263382). Cells lacking this gene are impaired in dephosphorylating McsB-P (PubMed:23770242). They also show a reduced resistance to ethanol stress (PubMed:15995210).
messages: Efficiently inhibited by Cu(2+) ion, Zn(2+) ion, sodium pyrophosphate and N-ethylmaleimide, while the addition of Mg(2+), Ca(2+) or Fe(3+) ions has minimal effect. Inhibited in a competitive manner by vanadate.
messages: Expression is up-regulated in the exponential phase of growth, followed by a significant and gradual reduction in the stationary/sporulation phase. Is not up-regulated during ethanol stress.
messages: Was originally thought to be a protein-tyrosine-phosphatase (PubMed:15995210). Was later shown to function as an arginine phosphatase in vivo and in vitro (PubMed:22517742, PubMed:23770242).

224308:BSU 27040
gene id: 224308:BSU_27040
uniprot id: A0A6M3ZET2
uniprot id: P26382
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIID domain, with its homologous EIIC domain, forms the PTS system translocation channel and contains part of its specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in fructose transport.

224308:BSU 35670
gene id: 224308:BSU_35670
uniprot id: A0A6M3ZL57
uniprot id: Q05852
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the UDPGP type 2 family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. Expressed under control of the sigma-B transcription factor.
messages: Catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP. This is an intermediate step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA). Has a role in the biosynthesis of all phosphate-containing envelope polymers, since UDP-glucose serves as a glucosyl donor not only for the biosynthesis of LTA but also for wall teichoic acids (WTAs). Is required for biofilm formation. This is likely due to another role of UDP-glucose, which might also act as a metabolic signal regulating biofilm formation or may be involved in some unknown biosynthetic pathway essential for biofilm formation, e.g. the synthesis of an exopolysaccharide.
messages: Cell membrane
messages: GtaB is the main UGPase enzyme producing UDP-glucose under both aerobic and anaerobic fermentative growth conditions (PubMed:33556370). YngB augments UDP-glucose production under anaerobic growth conditions (PubMed:33556370).
messages: Interacts with FloT.
messages: Membrane raft
messages: Under aerobic growth condition, deletion mutants display morphological defects and the cells are curled and show some bulges (PubMed:33556370). No aberrant morphology is observed for the deletion mutants under anaerobic growth conditions (PubMed:33556370).

224308:BSU 36290
gene id: 224308:BSU_36290
uniprot id: P94592
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.
messages: Catalyzes the dephosphorylation of phosphorylated 5-6 carbon sugars and monophosphate nucleotides (NMP) in vitro (By similarity). To a lesser extent, dephosphorylates flavin mononucleotide (FMN) in vitro (PubMed:26316208).

224308:BSU 07070
gene id: 224308:BSU_07070
uniprot id: O31528
names: Function
names: Induction
names: Similarity
messages: Belongs to the 'GDSL' lipolytic enzyme family.
messages: May play a role in the degradation of rhamnogalacturonan derived from plant cell walls. Probably has broad substrate specificity and may degrade several types of acetylated substrates.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 26110
gene id: 224308:BSU_26110
uniprot id: P45924
names: Similarity
messages: To B.subtilis XkdH.

224308:BSU 32810
gene id: 224308:BSU_32810
uniprot id: A0A6M4JM08
uniprot id: O32175
names: Similarity
messages: Belongs to the ArsC family.

224308:BSU 38420
gene id: 224308:BSU_38420
uniprot id: P15401
names: Function
names: PTM
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: In the presence of sucrose, SacY is activated and prevents premature termination of transcription by binding to a RNA-antiterminator (RAT) sequence (partially overlapping with the terminator sequence) located upstream of the sacB gene. Formation of the SacY-RAT complex prevents alternative formation of the terminator, allowing transcription of the sacB gene. In the absence of sucrose, inhibition of SacY activity by SacX leads to termination of transcription.
messages: Phosphorylated by HPr (PtsH). Also phosphorylated by SacX on His-99, leading to its inactivation.

224308:BSU 05030
gene id: 224308:BSU_05030
uniprot id: P96650
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00660
gene id: 224308:BSU_00660
uniprot id: P37562
names: Similarity
messages: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

224308:BSU 27850
gene id: 224308:BSU_27850
uniprot id: A0A6M3ZF07
uniprot id: Q9KWZ1
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the quinolinate synthase family. Type 3 subfamily.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.
messages: Cytoplasm
messages: Homotrimer.
messages: Mutant requires exogenous nicotinic acid for growth.

224308:BSU 12490
gene id: 224308:BSU_12490
uniprot id: A0A6M4JFJ6
uniprot id: O34423
names: Similarity
messages: Belongs to the manganese catalase family.

224308:BSU 31230
gene id: 224308:BSU_31230
uniprot id: A0A6M4JLK3
uniprot id: P39217
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase.
messages: Membrane

224308:BSU 39920
gene id: 224308:BSU_39920
uniprot id: A0A6M3ZI13
uniprot id: P42113
names: Similarity
messages: Belongs to the asparagine synthetase family.

224308:BSU 13110
gene id: 224308:BSU_13110
uniprot id: A0A6M3Z9Y6
uniprot id: O34990
names: Function
names: Similarity
messages: Belongs to the PurU family.
messages: Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).

224308:BSU 07490
gene id: 224308:BSU_07490
uniprot id: O34510
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Part of the ABC transporter complex YfmCDEF involved in citrate-dependent Fe(3+) import. Responsible for energy coupling to the transport system (Probable).
messages: The complex is composed of one ATP-binding protein (YfmF), two transmembrane proteins (YfmD and YfmE) and a solute-binding protein (YfmC).

224308:BSU 06880
gene id: 224308:BSU_06880
uniprot id: O31512
names: Similarity
messages: Belongs to the NmrA-type oxidoreductase family.

224308:BSU 19950
gene id: 224308:BSU_19950
uniprot id: A0A6M3ZCQ6
uniprot id: P02958
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the alpha/beta-type SASP family.
messages: SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.
messages: SASP are degraded in the first minutes of spore germination and provide amino acids for both new protein synthesis and metabolism.

224308:BSU 19350
gene id: 224308:BSU_19350
uniprot id: A0A6M3ZBZ5
uniprot id: O34524
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.
messages: Cell membrane
messages: Membrane

224308:BSU 16760
gene id: 224308:BSU_16760
uniprot id: A0A6M3ZAY7
uniprot id: Q04795
names: Activity Regulation
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aspartokinase family.
messages: Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.
messages: Diaminopimelate-sensitive.
messages: Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known).

224308:BSU 36430
gene id: 224308:BSU_36430
uniprot id: O32276
names: Function
messages: Required for translation of SpoIIID.

224308:BSU 12910
gene id: 224308:BSU_12910
uniprot id: A0A6M3ZAF4
uniprot id: Q00777
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the pyrroline-5-carboxylate reductase family.
messages: Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.
messages: Cytoplasm
messages: The proG proH proI triple mutant is auxotrophic for proline.

224308:BSU 40120
gene id: 224308:BSU_40120
uniprot id: O32292
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane.

224308:BSU 20610
gene id: 224308:BSU_20610
uniprot id: O34359
names: Caution
messages: Although it shares some weak sequence similarity with the UPF0398 family, it is distinct form other members of the family.

224308:BSU 34040
gene id: 224308:BSU_34040
uniprot id: A0A6M3ZG44
uniprot id: O07021
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the LutB/YkgF family.
messages: Cells lacking this gene are unable to grow on minimal medium with L-lactate as the sole carbon source. Cells lacking the lutABC operon exhibit little or no defect in biofilm formation on MSgg medium, but form small colonies that almost completely lacked surface architecture when glycerol is replaced with L-lactate in the MSgg medium.
messages: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofilm formation, since it contributes to the formation of architecturally complex communities when lactate is present.
messages: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate.
messages: Is under the dual control of the transcriptional repressors LutR and SinR, which allows the lutABC operon to be induced during both growth in liquid culture and biofilm formation. Is induced by L-lactate, which relieves LutR repression.

224308:BSU 03760
gene id: 224308:BSU_03760
uniprot id: A0A6M4JK14
uniprot id: P94414
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Could be member of the two-component regulatory system YclK/YclJ. Potentially phosphorylates YclJ.
messages: Membrane

224308:BSU 36210
gene id: 224308:BSU_36210
uniprot id: P96720
names: Disruption Phenotype
messages: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon has no visible growth phenotype, however it is out-competed by wild-type cells.

224308:BSU 15680
gene id: 224308:BSU_15680
uniprot id: A0A6M3ZE62
uniprot id: O34328
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the guanylate kinase family.
messages: Cytoplasm
messages: Essential for recycling GMP and indirectly, cGMP.

224308:BSU 11470
gene id: 224308:BSU_11470
uniprot id: A0A6M4JH02
uniprot id: P24137
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Component of the oligopeptide permease, a binding protein-dependent transport system. Necessary for genetic competence but not sporulation. Probably responsible for energy coupling to the transport system.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 22510
gene id: 224308:BSU_22510
uniprot id: A0A6M3ZDE2
uniprot id: P42978
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 23710
gene id: 224308:BSU_23710
uniprot id: A0A6M3ZDS1
uniprot id: P54560
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DNA polymerase type-Y family.
messages: Binds 2 magnesium ions per subunit.
messages: Cytoplasm
messages: Monomer.
messages: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).
messages: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.

224308:BSU 22580
gene id: 224308:BSU_22580
uniprot id: A0A6M3ZD32
uniprot id: P54390
names: Similarity
messages: Belongs to the UPF0302 family.

224308:BSU 38470
gene id: 224308:BSU_38470
uniprot id: P25152
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M28 family. M28B subfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Catalyzes the hydrolysis of a range of N-terminal amino acids.
messages: Monomer.
messages: Secreted

224308:BSU 06320
gene id: 224308:BSU_06320
uniprot id: A0A6M4JKR7
uniprot id: P46348
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.
messages: Cell membrane
messages: Deletion of the gene has no significant effet. However, a mneP-mneS double mutant is manganese sensitive and accumulates high levels of intracellular manganese.
messages: Membrane
messages: Secondary manganese efflux system. May prevent manganese intoxication.
messages: Transcriptionally activated by MntR in response to manganese excess. Expressed at a basal level in the absence of MntR.

224308:BSU 25110
gene id: 224308:BSU_25110
uniprot id: A0A6M3ZDZ1
uniprot id: P54478
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 28130
gene id: 224308:BSU_28130
uniprot id: A0A6M3ZEN9
uniprot id: P30950
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ALAD family.
messages: Binds 1 zinc ion per monomer.
messages: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
messages: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
messages: Homooctamer.

224308:BSU 30210
gene id: 224308:BSU_30210
uniprot id: A0A6M4JKL5
uniprot id: P53558
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the dethiobiotin synthetase family.
messages: Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 32440
gene id: 224308:BSU_32440
uniprot id: A0A6M3ZGA9
uniprot id: O32140
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
messages: Cell membrane
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced by TnrA during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or uric acid are added during limiting-nitrogen conditions.
messages: Membrane
messages: Uptake of uric acid.

224308:BSU 16100
gene id: 224308:BSU_16100
uniprot id: A0A6M4JGN9
uniprot id: P80865
names: Function
names: Similarity
names: Subunit
messages: Belongs to the succinate/malate CoA ligase alpha subunit family.
messages: Heterotetramer of two alpha and two beta subunits.
messages: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.

224308:BSU 39480
gene id: 224308:BSU_39480
uniprot id: A0A6M4JQU2
uniprot id: P54954
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Probably part of the ABC transporter complex YxeMNO that could be involved in amino-acid import. May transport S-methylcysteine. Responsible for energy coupling to the transport system (Probable).
messages: The complex is composed of two ATP-binding proteins (YxeO), two transmembrane proteins (YxeN) and a solute-binding protein (YxeM).

224308:BSU 04910
gene id: 224308:BSU_04910
uniprot id: O31491
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31670
gene id: 224308:BSU_31670
uniprot id: P14205
names: Function
names: Similarity
messages: Belongs to the thioesterase PaaI family.
messages: Is not required for competence.

224308:BSU 12300
gene id: 224308:BSU_12300
uniprot id: O34808
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily. Uronate isomerase family.
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 02200
gene id: 224308:BSU_02200
uniprot id: O31447
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FadG family.
messages: Cell membrane

224308:BSU 10550
gene id: 224308:BSU_10550
uniprot id: A0A6M3Z955
uniprot id: O07566
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the DegT/DnrJ/EryC1 family.
messages: Homodimer.
messages: Induced by neotrehalosadiamine.
messages: Involved in the biosynthesis of kanosamine (3-amino-3-deoxy-D-glucose), which is known to have antibiotic and antifungal properties, and to be a precursor of the antibiotic neotrehalosadiamine (3,3'-diamino-3,3'-dideoxy-alpha,beta-trehalose (NTD)). Catalyzes the reversible pyridoxal phosphate-dependent transamination of 3-dehydro-alpha-D-glucose 6-phosphate to form alpha-D-kanosamine-6-phosphate. It can only use alpha-anomer and glutamate is the only amino donor.
messages: The production of neotrehalosadiamine is dormant in the wild-type strain. A mutation in the beta subunit of RNA polymerase activates the production of the neotrehalosadiamine (PubMed:14612444).

224308:BSU 17870
gene id: 224308:BSU_17870
uniprot id: Q45057
names: Induction
names: Similarity
messages: Belongs to the site-specific recombinase resolvase family.
messages: Repressed by LexA.

224308:BSU 05090
gene id: 224308:BSU_05090
uniprot id: A0A6M3Z8C6
uniprot id: P96656
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 31010
gene id: 224308:BSU_31010
uniprot id: A0A6M4JKU9
uniprot id: O32076
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A hairpin loop (about residues 4-24) tethers the protein in the inner membrane. The isolated PHB domain (also called SPFH) oligomerizes, but does not bind lipids (PubMed:25635948). The C-terminal 24 residues are not required for correct localization, but the last 300 residues are required (PubMed:22753055). The C-terminus determines the oligomerization state of the protein; there are few large foci for FloT. Swapping with the C-terminus of FloA leads to many smaller foci (PubMed:25909364).
messages: Belongs to the band 7/mec-2 family. Flotillin subfamily.
messages: Cell membrane
messages: Delay in sporulation onset, 65% reduction in sporulation efficiency (PubMed:19383680, PubMed:22882210). No effect on KinC activity, a double floT-floA deletion decreases the number of proteins in the DRM, blocks the ability of KinC to stimulate biofilm formation (PubMed:20713508). Single floT deletion has defective motility, loss of NfeD2 localization, no change in FloA localization. Double floA-floT mutants have marked defects in cell morphology, motility, and transformation efficiency (PubMed:22753055). Double floA-floT deletion makes no biofilm, has greatly reduced FtsH, sporulates less than either single mutant (PubMed:22882210). Double dynA-floT deletions are highly elongated, filamentous and have strong defects in cell shape; cells grow very slowly with an extended lag phase (PubMed:23249255). Single mutation has a decrease in membrane fluidity and 35% decrease in protein secretion, a double floT-floA deletion has a stronger decrease in membrane fluidity and the same decrease in protein secretion (PubMed:23651456). Double dynA-floT deletion strains are less motile than single floT deletions (PubMed:26842743). Double floA-floT deletion has reduced oligomerization of KinC (PubMed:26297017). Double floA-floT deletion cells are somewhat elongated, the site of cell wall synthesis is affected, increasing at division septa. The speed of MreB movement around the cell is significantly decreased in rich medium in the floA-floT mutant (PubMed:32662773).
messages: Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and increase in number as cells age. FloA and FloT function is partially redundant; double deletions have marked synthetic phenotypes (PubMed:20713508, PubMed:22753055, PubMed:25909364, PubMed:27362352). Flotillins are thought to be important factors in membrane fluidity, especially during periods of rapid growth in rich media (Probable). Whether specific proteins are associated with FMMs is controversial; in one study FloT rafts have been shown to include proteins involved in adaptation to stationary phase, while FloA-FloT rafts include proteins involved in differentiation including sporulation, biofilm formation and DNA uptake competence. Another (more finely resolved) study only showed association of NfeD2 with FloT rafts of all the proteins examined (PubMed:25909364, PubMed:27362352). Aids homooligomerization of KinC and KinD but not KinB, may prevent incorrect hetero-association of the above kinases (PubMed:26297017). Simultaneous overexpression of both FloA and FloT leads to defects in cell division and differentiation, in part caused by stabilization of FtsH and its subsequent increased ability to degrade proteins. Cells make more biofilm, are about half as long, have less EzrA and more frequent Z-rings (PubMed:24222488). Involved in spatial organization of membranes, perhaps recruiting proteins (e.g. NfeD2) to specific membrane regions (Probable) (PubMed:23651456). Plays a role in phosphorylation of master regulator Spo0A, an early sporulation event (PubMed:19383680). Plays a non-redundant role with dynamin-like protein A (dynA) in membrane dynamics and cell shape (PubMed:23249255).
messages: Homooligomerizes (PubMed:25909364). Oligomerizes in very large complexes in vitro. Interacts with FloA, FtsH, FtsX, OppA, SdhA and SecY in detergent-resistant membrane (DRM) fractions (PubMed:23651456). Interacts with FtsH at midcell (Probable). Interacts with FloA (PubMed:25909364). Interacts in vivo with KinC, FloA, FtsH and ResE (PubMed:26297017). Interacts with ResE, colocalizes with ResE in FloT-only membrane rafts (PubMed:25909364). Another study shows nearly complete colocalization with NfeD2, but only minor colocalization with FtsH or KinC (PubMed:27362352).
messages: Membrane raft
messages: Transcription starts during stationary phase. Few foci are seen in exponential phase cells; the number of foci increases as cells enter stationary phase (at protein level) (PubMed:19383680, PubMed:23651456, PubMed:22753055). Few foci are seen on rich media, when cells are grown in minimal medium more foci are seen (at protein level) (PubMed:22753055). Expressed at low levels in rich media during exponential growth, more highly expressed in stationary phase on sporulation/biofilm-inducing media, activated by spo0A probably via AbrB (at protein level). Surfactin induces expression via spo0A (PubMed:25909364).

224308:BSU 14569
gene id: 224308:BSU_14569
uniprot id: A0A6M3ZAA0
uniprot id: C0H409
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Membrane

224308:BSU 00570
gene id: 224308:BSU_00570
uniprot id: A0A6M4JC95
uniprot id: P37555
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide synthase family.
messages: Cell membrane
messages: Membrane

224308:BSU 12760
gene id: 224308:BSU_12760
uniprot id: P54342
names: Similarity
messages: To B.subtilis YqcC.

224308:BSU 34470
gene id: 224308:BSU_34470
uniprot id: A0A6M3ZKZ5
uniprot id: O07002
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Allows enhanced growth with L-aspartate as a sole source nitrogen source.
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. AGT (TC 2.A.3.11) family.
messages: Cell membrane
messages: Induced by sucrose.
messages: Inhibited by carbonyl cyanide m-chlorophenylhydrazone and carbonyl cyanide 4-trifluoro-methoxyphenylhydrazone.
messages: Membrane
messages: Uptake of L-aspartate with the concomitant import of a proton. Can also transport aspartate hydroxamate and L-glutamate with lower affinity and efficiency.

224308:BSU 03020
gene id: 224308:BSU_03020
uniprot id: A0A6M3ZCF4
uniprot id: P55908
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: To E.coli YfcC and H.influenzae HI_0594.

224308:BSU 09200
gene id: 224308:BSU_09200
uniprot id: P54603
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial sortase family. Class D subfamily.
messages: Cell membrane
messages: Mutant releases elevated levels of the sortase substrate YfkN into the culture medium upon phosphate starvation (PubMed:21800427). Inactivation of the gene abolishes the cell wall anchoring of a fusion protein composed of a beta-lactamase reporter and the C-terminal region of the sortase substrate YhcR (PubMed:22020651).
messages: Preferentially expressed in the late stationary phase.
messages: Transpeptidase that anchors surface proteins to the cell wall (PubMed:21906378, PubMed:21800427, PubMed:22020651). Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall (Probable). This sortase recognizes a Leu-Pro-Asp-Thr-Ser/Ala (LPDTS/A) motif (PubMed:21906378, PubMed:22020651). It has two substrates, YhcR and YfkN (PubMed:21906378, PubMed:21800427, PubMed:22020651).

224308:BSU 25480
gene id: 224308:BSU_25480
uniprot id: A0A6M4JL48
uniprot id: P15874
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the GrpE family.
messages: Cytoplasm
messages: Homodimer.
messages: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

224308:BSU 31660
gene id: 224308:BSU_31660
uniprot id: A0A6M4JP00
uniprot id: O05227
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit G family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: May enhance MrpA stability, assembly, or function. May play chaperone or assembly roles for MrpA and perhaps for other mrp proteins.
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 09710
gene id: 224308:BSU_09710
uniprot id: A0A6M3Z8Z2
uniprot id: O07550
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Heterodimer composed of YheH and YheI.
messages: Induced by several classes of structurally unrelated antibiotics, such as erythromycin, fusidic acid or linezolid.
messages: Inhibited by ortho-vanadate.
messages: Involved in the transport of four structurally unrelated drugs, including doxorubicin and mitoxantrone. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation.
messages: Membrane
messages: Overexpression reduces the sporulation efficiency possibly by modulating the function of KinA.

224308:BSU 09050
gene id: 224308:BSU_09050
uniprot id: P54589
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 19450
gene id: 224308:BSU_19450
uniprot id: O31856
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Cells lacking this gene have no visible phenotype in response to salt, ethanol or energy stress. However cells with multiple disruptions (RsbRA, RsbRB, RsbRC and RsbRD) have an increased basal level of sigma-B, indicating this protein is a negative regulator of sigma-B.
messages: Negative regulator of sigma-B activity. Non-phosphorylated RsbS binds to RsbT, preventing its association with RsbU. Requires any one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and the RsbR paralog(s) are phosphorylated, they release RsbT, which can then bind and activate RsbU.
messages: One of 4 functionally non-identical RsbR paralogs, it functions in the environmental signaling branch of the general stress response.
messages: Phosphorylated by RsbT.
messages: Probably present in the stressosome with RsbRA, RsbRB, RsbRD and RsbS.

224308:BSU 36610
gene id: 224308:BSU_36610
uniprot id: P71038
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 21329
gene id: 224308:BSU_21329
uniprot id: Q9K3A9
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29660
gene id: 224308:BSU_29660
uniprot id: A0A6M4JNQ7
uniprot id: P21466
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS4 family.
messages: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.
messages: Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.
messages: S4 represses its own expression; it is not know if this is at the level of translation or of mRNA stability.
messages: With S5 and S12 plays an important role in translational accuracy.
messages: With S5 and S12 plays an important role in translational accuracy; many suppressors of streptomycin-dependent mutants of protein S12 are found in this protein, some but not all of which decrease translational accuracy (ram, ribosomal ambiguity mutations).

224308:BSU 26075
gene id: 224308:BSU_26075
uniprot id: A0A6M4JL37
uniprot id: P45927
names: Similarity
messages: Belongs to the myoviridae tail sheath protein family.

224308:BSU 14930
gene id: 224308:BSU_14930
uniprot id: A0A6M3ZAF6
uniprot id: O34329
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 25460
gene id: 224308:BSU_25460
uniprot id: A0A6M3ZEL7
uniprot id: P17631
names: Cofactor
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DnaJ family.
messages: Binds 2 Zn(2+) ions per monomer.
messages: By heat shock.
messages: Cytoplasm
messages: Homodimer.
messages: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
messages: The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.

224308:BSU 40390
gene id: 224308:BSU_40390
uniprot id: Q794W0
names: Disruption Phenotype
names: Domain
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homodimer (By similarity). Interacts with WalK and YycI.
messages: No cellular levels increase of either WalK or WalR. Induction of WalR-dependent gene expression. Cell wall defect.
messages: The transmembrane region is required for the regulation of WalK activity.
messages: Together with YycI, regulates the activity of the two-component system WalR/WalK.

224308:BSU 28570
gene id: 224308:BSU_28570
uniprot id: A0A6M4JKT9
uniprot id: P94546
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0719 family.
messages: Cell membrane
messages: Membrane

224308:BSU 08850
gene id: 224308:BSU_08850
uniprot id: A0A6M3ZC57
uniprot id: P40401
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system for aliphatic sulfonates. Probably responsible for the translocation of the substrate across the membrane.
messages: Repressed by sulfate and cysteine.

224308:BSU 02470
gene id: 224308:BSU_02470
uniprot id: A0A6M4JCE8
uniprot id: P42236
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aldehyde dehydrogenase family.
messages: Catalyzes the NAD(P)(+)-dependent oxidation of alpha-ketoglutaric semialdehyde (alphaKGSA) to alpha-ketoglutarate. Prefers NADP(+) to NAD(+) as a cosubstrate. In vitro, can also use various aldehydes.
messages: Homotetramer.
messages: No effect on vanillin degradation.

224308:BSU 29290
gene id: 224308:BSU_29290
uniprot id: O34980
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the peptidase M20 family.
messages: Binds 2 divalent metal cations per subunit (By similarity). Is most efficient with Zn(2+) and Co(2+), has only a small activity with Ni(2+) and Mn(2+) and no activity with Mg(2+), Fe(3+), and Fe(2+) (PubMed:35584544).
messages: Involved in a cysteine salvage pathway from S-alkylcysteine. Catalyzes the last step in this pathway, i.e. the deacetylation of N-acetyl-L-cysteine. This pathway is likely important in the catabolism of alkylated cysteine generated by proteolysis of alkylated glutathione formed in the detoxification of a wide range of electrophiles.

224308:BSU 17830
gene id: 224308:BSU_17830
uniprot id: O31816
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30110
gene id: 224308:BSU_30110
uniprot id: C0SP80
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: May play a role in the degradation of type I rhamnogalacturonan derived from plant cell walls.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 22320
gene id: 224308:BSU_22320
uniprot id: P39793
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Required for vegetative growth (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth (PubMed:9851991).
messages: Cells require increased levels of Mg(2+) or Ca(2+) for growth and germination. Approximately 50% of cells without the gene contain abnormal FtsZ rings, suggesting it is involved in septum synthesis; increased levels of Mg(2+) or Ca(2+) only partially eliminate the septation defects (PubMed:9721295). Double ponA-pbpA deletions spores have greatly decreased viability, peptidoglycan synthesis and elongate poorly; increased levels of Mg(2+) increase spore viability (PubMed:9851991).
messages: Expression is constant during growth, decreases during sporulation and is induced approximately 15 min into spore germination. Present in the inner forespore membrane of the dormant spore (PubMed:3080407).
messages: Forespore inner membrane
messages: In the C-terminal section; belongs to the transpeptidase family.
messages: In the N-terminal section; belongs to the glycosyltransferase 51 family.
messages: The N-terminus is blocked.
messages: The product expressed from the translation of the ponA gene appears as two bands on a gel (1A and 1B), but the specific amino acid sequence of each protein is unknown.

224308:BSU 35100
gene id: 224308:BSU_35100
uniprot id: O34648
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 09100
gene id: 224308:BSU_09100
uniprot id: A0A6M4JHQ8
uniprot id: P32081
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: A double cshB-cspB disruption mutant cannot be made.
messages: Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
messages: Cytoplasm
messages: Homodimer. Interacts with RNA helicase CshB when cells are transcriptionally active.
messages: In response to low temperature.
messages: nucleoid

224308:BSU 39360
gene id: 224308:BSU_39360
uniprot id: A0A6M3ZI34
uniprot id: P25503
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the urocanase family.
messages: Binds 1 NAD(+) per subunit.
messages: Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.
messages: Composed of at least two subunits.
messages: Cytoplasm

224308:BSU 25600
gene id: 224308:BSU_25600
uniprot id: A0A6M4JMI1
uniprot id: P39696
names: Function
names: Similarity
messages: Belongs to the pyrroline-5-carboxylate reductase family.
messages: Dispensable for transformability. Not known if it can act as a pyrroline-5-carboxylate reductase.

224308:BSU 35740
gene id: 224308:BSU_35740
uniprot id: P27623
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytidylyltransferase family.
messages: Catalyzes the transfer of the cytidylyl group of CTP to sn-glycerol 3-phosphate so the activated glycerol 3-phosphate can be used for teichoic acid synthesis, via incorporation into both the linkage unit and the teichoic acid polymer by TagB and TagF.
messages: Cytoplasm
messages: Homodimer.
messages: Inhibited by the divalent cations cadmium, mercury, tin, copper and zinc.
messages: Positively regulated by WalR. Mainly expressed during exponential growth and rapidly shut off as cells enter the stationary phase.
messages: Proceeds via a random order reaction mechanism where there is negative cooperativity in the binding of substrates but not in catalysis.

224308:BSU 18130
gene id: 224308:BSU_18130
uniprot id: A0A6M3ZBL5
uniprot id: P10475
names: Similarity
messages: Belongs to the glycosyl hydrolase 5 (cellulase A) family.

224308:BSU 16780
gene id: 224308:BSU_16780
uniprot id: A0A6M3ZAU7
uniprot id: O31760
names: Cofactor
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.
messages: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.
messages: Binds 1 zinc ion per subunit. The inability to bind a second zinc ion may explain its very poor exonuclease activity (PubMed:21893285).
messages: Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.
messages: Cytoplasm
messages: Endonucleolytically cleaves the 5'-leader sequence of certain mRNAs. Endonuclease digestion by the RNase J1/J2 complex occurs at a different site and in some cases more efficiently than J1 or J2 alone. The exonuclease activity of the J1/J2 complex is highly processive on substrates longer than 5 nucleotides, on shorter substrates is distributive. Plays a role in mRNA maturation and stability. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to RNase J1. This subunit alone has very poor 5'-3' exonuclease activity.
messages: Homodimer, may be a subunit of the RNA degradosome.
messages: Not essential. While depletion/deletion of RNase J1 or J2 has no large impact on global gene expression, a double mutant alters the expression of hundreds of genes (PubMed:18713320).
messages: Present in about 3000 monomers per cell in mid-log phase.
messages: Unclear whether it forms homodimers or belongs to a larger complex. According to (PubMed:20025672) probably does not form homodimers, while (PubMed:21893285) shows homodimer formation. Both reports show RNase J1 and J2 interaction, probably as a heterotetramer (PubMed:19193632) shows it is a component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno, while (PubMed:20025672) finds no evidence of an RNA degradosome complex.

224308:BSU 13630
gene id: 224308:BSU_13630
uniprot id: A0A6M3ZAM3
uniprot id: O31670
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 04210
gene id: 224308:BSU_04210
uniprot id: P96579
names: Function
names: Similarity
names: Subunit
messages: Belongs to the acetyltransferase family.
messages: Homohexamer, and homodimer.
messages: Putative N-acetyltransferase. May act on ribosomal proteins (Potential).

224308:BSU 33320
gene id: 224308:BSU_33320
uniprot id: P37580
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Membrane raft
messages: Part of the ABC transporter complex FhuCBGD involved in iron(3+)-hydroxamate import. Binds the iron(3+)-hydroxamate complex and transfers it to the membrane-bound permease. Required for the transport of ferrichrome and coprogen.
messages: The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB and FhuG) and a solute-binding protein (FhuD or YxeB).

224308:BSU 13180
gene id: 224308:BSU_13180
uniprot id: A0A6M3Z9X4
uniprot id: P80877
names: Cofactor
names: Function
names: Induction
names: PTM
names: Similarity
messages: Belongs to the vitamin-B12 independent methionine synthase family.
messages: Binds 1 zinc ion per subunit.
messages: Binds 2 Zn(2+) ions per subunit.
messages: By superoxide.
messages: Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
messages: In response to oxidative stress, Cys-719 can react with bacillithiol (BSH) to form mixed disulfides. S-bacillithiolation leads to loss of catalytic activity and methionine auxotrophy.

224308:BSU 22360
gene id: 224308:BSU_22360
uniprot id: A0A6M3ZM76
uniprot id: P39772
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 29100
gene id: 224308:BSU_29100
uniprot id: P23545
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Member of the two-component regulatory system PhoP/PhoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoP in response to environmental signals.
messages: Membrane raft
messages: Oligomerizes, probably forms homodimers. Interacts with FloA, colocalizes with FloA-only membrane rafts. Oligomerization is assisted by FloA (PubMed:25909364). Another study shows only minor colocalization with FloA or FloT (PubMed:27362352).

224308:BSU 36870
gene id: 224308:BSU_36870
uniprot id: A0A6M3ZLC2
uniprot id: P37813
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase A chain family.
messages: Cell membrane
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.
messages: Membrane

224308:BSU 19400
gene id: 224308:BSU_19400
uniprot id: A0A6M4JJI4
uniprot id: O31851
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Cu-Zn superoxide dismutase family.
messages: Binds 1 zinc ion per homodimer. The zinc ion is bound between 2 subunits and mediates dimerization.
messages: Cell membrane
messages: Monomer, and homodimer. Largely unstructured monomer in solution. Well-ordered homodimer in the crystal.

224308:BSU 21600
gene id: 224308:BSU_21600
uniprot id: O32000
names: Similarity
messages: Belongs to the cry6A endotoxin family.

224308:BSU 00200
gene id: 224308:BSU_00200
uniprot id: A0A6M4JBZ5
uniprot id: P24281
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the YbaB/EbfC family.
messages: Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.
messages: Homodimer.
messages: nucleoid

224308:BSU 15410
gene id: 224308:BSU_15410
uniprot id: P71020
names: Disruption Phenotype
names: Miscellaneous
messages: No alteration in cell division.
messages: YlmH and DivIVA are functionally unrelated.

224308:BSU 28720
gene id: 224308:BSU_28720
uniprot id: A0A6M4JKK3
uniprot id: P94531
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 51 family.
messages: Cells lacking this gene retain 38% of arabinofuranosidase activity, and the double mutant abfA/abf2 is inactive.
messages: Cytoplasm
messages: Homohexamer; trimer of dimers.
messages: Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in different hemicellulosic homopolysaccharides (branched and debranched arabinans). It acts preferentially on arabinotriose, arabinobiose and linear alpha-(1->5)-L-arabinan, and is much less effective on branched sugar beet arabinan.
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene (Probable).

224308:BSU 07850
gene id: 224308:BSU_07850
uniprot id: A0A6M4JHD8
uniprot id: P80876
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the peptidase C56 family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Functions in the protection against aldehyde-stress, possibly by degrading damaged proteins.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: When combined with a yraA disruption shows significantly reduced growth in the presence of formaldehye and methylglyoxal.

224308:BSU 05540
gene id: 224308:BSU_05540
uniprot id: A0A6M3Z869
uniprot id: P96697
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0702 family.
messages: Cell membrane
messages: Membrane

224308:BSU 35600
gene id: 224308:BSU_35600
uniprot id: A0A6M4JRR6
uniprot id: O32273
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide synthase family.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: Cell membrane
messages: Membrane
messages: Might be involved in the translocation of teichuronic acid repeating units from the inner to the outer surface of the membrane.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 28410
gene id: 224308:BSU_28410
uniprot id: P11470
names: Function
messages: Involved in the regulation of spore formation. Directs the transcription of several genes that encode structural components of the protein coat that encases the mature spore (CotB, CotC, CotG, CotS, CotV, CotW, CotX, CotY and CotZ). Controls also the cgeAB and cgeCDE operons.

224308:BSU 15750
gene id: 224308:BSU_15750
uniprot id: A0A6M3ZAM7
uniprot id: O34617
names: Caution
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the radical SAM superfamily. RlmN family.
messages: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.
messages: Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

224308:BSU 12450
gene id: 224308:BSU_12450
uniprot id: A0A6M4JHM7
uniprot id: O34446
names: Similarity
messages: Belongs to the YciI family.

224308:BSU 13660
gene id: 224308:BSU_13660
uniprot id: A0A6M4JHL1
uniprot id: O31671
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Cells lacking this gene sporulate normally in spo0F mutants.
messages: Expressed during growth and early stationary phase.
messages: Membrane
messages: Oligomerizes, probably forms homodimers; oligomerization is assisted by FloT. Interacts with FloT.
messages: Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions.

224308:BSU 17880
gene id: 224308:BSU_17880
uniprot id: A0A6M4JJ35
uniprot id: O31818
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0291 family.
messages: Cytoplasm
messages: Repressed by LexA.

224308:BSU 08010
gene id: 224308:BSU_08010
uniprot id: O31544
names: Function
names: Similarity
messages: Belongs to the alkylbase DNA glycosidase AlkA family.
messages: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.

224308:BSU 35310
gene id: 224308:BSU_35310
uniprot id: A0A6M3ZJP2
uniprot id: P28368
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.
messages: Cytoplasm
messages: Expressed under the dual control of sigma-B (PubMed:9296790) and sigma-H factors (PubMed:9296790, PubMed:9852014). The sigma-B-dependent promoter drives expression of yvyD under stress conditions and after glucose starvation, whereas a sigma-H-dependent promoter is responsible for yvyD transcription following amino acid depletion (PubMed:9852014). Also claimed to be under sole control of sigma-H (PubMed:22950019).
messages: Interacts with 100S ribosomes.
messages: Interacts with 100S ribosomes. Not associated with 70S ribosome monomers, about 1 monomer per ribosome (PubMed:22950019).
messages: Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.
messages: Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth. May not be the only factor implicated (PubMed:22950019). Might negatively regulate the activity of the sigma-54 factor (SigL) (PubMed:9852014).
messages: The expression of a sigL-dependent gene (levD) is up-regulated two-fold in cells lacking yvyD, but the relative amount of the sigL transcript is not increased (PubMed:9852014). In cells overexpressing ppGpp synthase YwaC, deletion of this gene causes loss of 100S ribosome formation by dimerizing 70S ribosomes (PubMed:22950019).

224308:BSU 17820
gene id: 224308:BSU_17820
uniprot id: O31815
names: Developmental Stage
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0714 family.
messages: Cell membrane
messages: Expressed during sporulation, and this requires the RNA polymerase sigma factor SigK.

224308:BSU 32150
gene id: 224308:BSU_32150
uniprot id: P21340
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the acetyltransferase family.
messages: Cells lacking this gene display release of repression of protease synthesis and sporulation in glucose-enriched medium.
messages: Involved in the protection against polyamine toxicity by regulating their concentration. Could also be involved in the negative control of sporulation as well as production of degradative enzymes such as alpha-amylase, levansucrase and alkaline phosphatase. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to an acceptor substrate and releases both CoA and the acetylated product. It possesses N1-acetyltransferase activity toward polyamine substrates including spermidine, spermine, aminopropylcadaverine, norspermidine, homospermidine, N(8)-acetylspermidine, diaminopropane and agmatine.
messages: Monomer.

224308:BSU 37110
gene id: 224308:BSU_37110
uniprot id: A0A6M4JQ40
uniprot id: P19669
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transaldolase family. Type 3B subfamily.
messages: Cytoplasm
messages: Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.
messages: Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway. Does not show fructose-6-P aldolase activity.

224308:BSU 23960
gene id: 224308:BSU_23960
uniprot id: A0A6M4JL58
uniprot id: P54537
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Impaired growth on arginine as the nitrogen source.
messages: Part of a binding-protein-dependent transport system for arginine. Probably responsible for energy coupling to the transport system.

224308:BSU 18030
gene id: 224308:BSU_18030
uniprot id: A0A6M3ZBK6
uniprot id: Q45060
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Tlp family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination. Expression is sigma F and sigma G-dependent.
messages: Spore core

224308:BSU 23140
gene id: 224308:BSU_23140
uniprot id: A0A6M4JKI9
uniprot id: P35161
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells lacking this gene exhibit a lack of cytochromes c but normal contents of cytochrome a and b. Can sporulate.
messages: Membrane
messages: Required for the biogenesis of c-type cytochromes.

224308:BSU 33990
gene id: 224308:BSU_33990
uniprot id: A0A6M3ZGE4
uniprot id: O32255
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 02880
gene id: 224308:BSU_02880
uniprot id: A0A6M4JF72
uniprot id: O34504
names: Similarity
messages: To bacterial alkanal monooxygenase alpha and beta chains.

224308:BSU 34690
gene id: 224308:BSU_34690
uniprot id: A0A6M4JPG5
uniprot id: O06981
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Membrane

224308:BSU 16460
gene id: 224308:BSU_16460
uniprot id: A0A6M4JIQ3
uniprot id: P40404
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the CheD family.
messages: Cells exhibit poorly methylated receptors, are tumbly and have a decreased sensitivity to attractants.
messages: Deamidates 'Gln-593' and 'Gln-594' of the chemoreceptor McpA. In addition, deamidates other chemoreceptors, including McpB and McpC. CheD-mediated MCP (methyl-accepting chemotaxis proteins) deamidation is required for productive communication of the conformational signals of the chemoreceptors to the CheA kinase. CheD is absolutely required for a behavioral response mediated by McpC but is not required for the response to asparagine mediated by McpB. CheD is necessary for the generation of wild-type prestimulus CheA autophosphorylation levels. Also required for methylation of MCPs by CheR. In addition, enhances the activity of CheC 5-fold in vitro.
messages: Deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs). CheD-mediated MCP deamidation is required for productive communication of the conformational signals of the chemoreceptors to the cheA kinase.
messages: Forms a complex with CheC.

224308:BSU 12390
gene id: 224308:BSU_12390
uniprot id: A0A6M3Z9K9
uniprot id: O34673
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the UxaA family.
messages: Catalyzes the dehydration of D-altronate.
messages: Induced by galacturonate, repressed by glucose.
messages: Last gene in the exu locus which is required for galacturonate utilization.

224308:BSU 37660
gene id: 224308:BSU_37660
uniprot id: A0A6M4JQB9
uniprot id: P39646
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phosphate acetyltransferase and butyryltransferase family.
messages: Cytoplasm

224308:BSU 33830
gene id: 224308:BSU_33830
uniprot id: A0A6M3ZH14
uniprot id: O34992
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Binds cyclic di-AMP (c-di-AMP), which may regulate the transporter activity.
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for energy coupling to the transport system.
messages: Repressed by OpcR.
messages: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).

224308:BSU 40930
gene id: 224308:BSU_40930
uniprot id: P37519
names: Cofactor
names: Similarity
messages: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.

224308:BSU 00150
gene id: 224308:BSU_00150
uniprot id: A0A6M4JER1
uniprot id: P37530
names: Activity Regulation
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the DCK/DGK family.
messages: Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism.
messages: Homodimer.
messages: Inhibited by deoxyguanosine at concentrations above 30 uM only with UTP as phosphate donor. dGTP is a potent competitive inhibitor.
messages: Plays an essential role in generating the deoxyribonucleotide precursors dGTP for DNA metabolism. Highly specific toward deoxyguanosine (dGuo) and deoxyinosine (dIno). Only marginal activity is observed with guanosine. UTP is slightly more efficient as phosphate donor than CTP, ATP and GTP.

224308:BSU 15930
gene id: 224308:BSU_15930
uniprot id: A0A6M3ZB29
uniprot id: P51833
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ribonuclease III family.
messages: Cytoplasm
messages: Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.
messages: Digests double-stranded RNA. Involved in the processing of ribosomal RNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes pre-scRNA (the precursor of the signal recognition particle RNA). Probably also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (PubMed:22229825, PubMed:26940229, PubMed:11123676, PubMed:17576666, PubMed:18363798, PubMed:22412379). Probably processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (Probable). Involved in degradation of type I toxin-antitoxin system duplex RNAs (PubMed:22229825, PubMed:26940229).
messages: Essential. Depletion leads to the accumulation of 30S precursor rRNA (PubMed:11123676), and alteration of levels of about 11% of total transcripts (PubMed:22412379, PubMed:11123676, PubMed:18363798). Increased half-life of type I toxin-antitoxin system RNAs of BsrG/SR4 and toxin BsrE mRNA (note these strains must have an essential second-site suppressor to survive) (PubMed:22229825, PubMed:26940229).
messages: Homodimer.

224308:BSU 13470
gene id: 224308:BSU_13470
uniprot id: A0A6M4JFL1
uniprot id: P04833
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the alpha/beta-type SASP family.
messages: SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.
messages: SASP are degraded in the first minutes of spore germination and provide amino acids for both new protein synthesis and metabolism.

224308:BSU 35000
gene id: 224308:BSU_35000
uniprot id: A0A6M3ZL24
uniprot id: O34483
names: Activity Regulation
names: Caution
names: Cofactor
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: According to PubMed:12779331, the mutation G152A reduces the kinase activity about twofold and has little effect on phosphorylase activity, whereas according to PubMed:12055300, it completely prevents both functions. This conflicting result may be explained by limiting amounts substrates conditions used in PubMed:12779331 assays.
messages: Belongs to the HPrK/P family.
messages: Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.
messages: Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-45' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Also phosphorylates/dephosphorylates the HPr-like catabolite repression protein crh on 'Ser-46'. Therefore, by controlling the phosphorylation state of HPr and crh, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.
messages: Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Also phosphorylates/dephosphorylates the HPr-like catabolite repression protein crh on a specific serine residue. Therefore, by controlling the phosphorylation state of HPr and crh, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.
messages: Constitutively expressed, with or without glucose in the growth medium.
messages: Homohexamer.
messages: Is active as a kinase only at high ATP concentrations or at low ATP concentrations in the presence of the allosteric activator fructose 1,6-bisphosphate (FBP). The pyrophosphate-dependent HPr phosphorylation is not stimulated by FBP. Kinase activity is inhibited by inorganic phosphate (Pi). Dephosphorylation of HPr(Ser-P) by B.subtilis HPrK/P becomes prevalent when the concentration of Pi increases. Thus, the kinase activity may prevail under conditions of good nutrient supply, whereas the phosphorylase activity is dominant if carbon and energy sources become limiting.
messages: The Walker A ATP-binding motif also binds Pi and PPi.
messages: The enzyme may harbor two different cation-binding sites, one that interacts specifically with the nucleotide, and the other that is involved in the binding of the protein substrate.
messages: Was originally called HPr kinase/phosphatase, but P-Ser-HPr dephosphorylation was found to follow a quite unique mechanism (PubMed:12359880), in which Pi instead of H(2)O is used for the nucleophilic attack on the phosphoryl group. P-Ser-HPr dephosphorylation is therefore not a phosphohydrolysis but a phospho-phosphorolysis reaction, and the bifunctional enzyme was dubbed HPr kinase/phosphorylase.

224308:BSU 19750
gene id: 224308:BSU_19750
uniprot id: P42093
names: Function
messages: May be involved in maturation of the outermost layer of the spore.

224308:BSU 31160
gene id: 224308:BSU_31160
uniprot id: A0A6M4JMS1
uniprot id: O32086
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.
messages: Cell membrane
messages: Membrane

224308:BSU 02580
gene id: 224308:BSU_02580
uniprot id: P42247
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32980
gene id: 224308:BSU_32980
uniprot id: A0A6M3ZG62
uniprot id: P37959
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 16960
gene id: 224308:BSU_16960
uniprot id: A0A6M4JGI3
uniprot id: O31774
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RNase Y family.
messages: Cell membrane
messages: Endoribonuclease that initiates mRNA decay.
messages: Endoribonuclease that initiates mRNA decay. Initiates the decay of all SAM-dependent riboswitches, such as yitJ riboswitch. Involved in processing of the gapA operon mRNA, it cleaves between cggR and gapA (PubMed:19193632). Is also the decay-initiating endonuclease for rpsO mRNA. Involved in degradation of type I toxin-antitoxin system bsrG/SR4 RNAs and a minor role in degradation of type I toxin-antitoxin system bsrE/SR5 degradation (PubMed:22229825, PubMed:26940229).
messages: Essential; depletion mutants show a significant increase in global mRNA half-life (PubMed:19779461) a decrease in at least 1 specific mRNA processing event (PubMed:19193632); in a more severe depletion experiment alteration of about 26% of transcripts was seen (PubMed:22412379). Later shown not to be essential in 4 strains, with a doubled doubling time, cells are translucent, suggesting a possible cell surface defect. 168 trpC2 cells able to grow on minimal medium. Loss of competence for plasmid transformation, 1000-fold less sporulation. Increased sensitivity to a wide range of antibiotics. Thinner cells form long curved structures of 2-3 cell lengths, cell walls are altered with looser, considerably less dense peptidoglycan. Double pnp-rny mutants grow very slowly, while rnjA-rny mutants could not be isolated (PubMed:23504012). Increased half-life of type I toxin-antitoxin system RNAs of BsrG/SR4 (PubMed:22229825).
messages: Has 5 domains: N-terminal transmembrane, coiled-coil, KH, HD and an unnamed conserved C-terminal domain (residues 430-520). Both the N-terminal transmembrane helix and C-terminal domain are required for protein function in vivo.
messages: Homodimer (Probable). Component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno (PubMed:19193632) (although rnjA and rnjB's presence is unclear). Interacts with RNA helicase CshA which may also be a member of the RNA degradosome complex (PubMed:20572937). Interacts with full-length dynamin-like protein DynA (PubMed:23060960).
messages: Magnesium. Can also use manganese or zinc.
messages: Shows preference for transcripts carrying a monophosphate group at the 5' end.

224308:BSU 16320
gene id: 224308:BSU_16320
uniprot id: A0A6M3ZBF8
uniprot id: P24073
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliN/MopA/SpaO family.
messages: Cell membrane
messages: Component of the flagellar switch. Binds CheY-P and increases its hydrolysis rate in vitro. May function constitutively to remove CheY-P around the flagellar switch to maintain an optimal level of CheY-P whereas CheC may function after addition of an attractant to cope with increased levels of CheY-P.
messages: FliN is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.
messages: Membrane

224308:BSU 02760
gene id: 224308:BSU_02760
uniprot id: A0A6M4JCI0
uniprot id: P46904
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Induced by ethanol and the protonophore carbonylcyanide p-chlorophenylhydrazone (CCCP) and, more modestly, by sodium and potassium. Positively regulated by the two-component regulatory system NatK/NatR.
messages: Membrane
messages: Part of an ABC transporter that catalyzes ATP-dependent electrogenic sodium extrusion.
messages: The complex is composed of NatA and NatB.

224308:BSU 07260
gene id: 224308:BSU_07260
uniprot id: A0A6M3ZID1
uniprot id: Q797B3
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LTA synthase family.
messages: Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate.
messages: Cell membrane
messages: Could not restore staphylococcal growth after ltaS depletion.
messages: Membrane
messages: Proteolytically cleaved by the type I signal peptidases SipT and SipV.
messages: Secreted
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 06910
gene id: 224308:BSU_06910
uniprot id: A0A6M4JE30
uniprot id: Q45538
names: Function
names: Similarity
messages: Belongs to the manganese catalase family.
messages: The cotJ operon proteins affect spore coat composition. They are either required for the normal formation of the inner layers of the coat or are themselves structural components of the coat.

224308:BSU 32060
gene id: 224308:BSU_32060
uniprot id: O32107
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38620
gene id: 224308:BSU_38620
uniprot id: A0A6M4JQI5
uniprot id: P94378
names: Similarity
messages: Belongs to the DNA glycosylase MPG family.

224308:BSU 18040
gene id: 224308:BSU_18040
uniprot id: Q45061
names: Function
names: Similarity
messages: Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.
messages: Has acyl-CoA thioesterase activity.

224308:BSU 20590
gene id: 224308:BSU_20590
uniprot id: O34581
names: Similarity
messages: Belongs to the HNH nuclease family.

224308:BSU 34450
gene id: 224308:BSU_34450
uniprot id: P05655
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 68 family.
messages: Induced by sucrose.
messages: Secreted

224308:BSU 15630
gene id: 224308:BSU_15630
uniprot id: O34813
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
messages: Catalyzes the dehydrogenation of precorrin-2 to form sirohydrochlorin which is used as a precursor in both siroheme biosynthesis and in the anaerobic branch of adenosylcobalamin biosynthesis.
messages: Cells lacking this gene are unable to grow on minimal medium with nitrate as nitrogen source. Growth is restored only after addition of both cysteine and ammonia to the medium.
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 33760
gene id: 224308:BSU_33760
uniprot id: A0A6M3ZG33
uniprot id: O34616
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By Spo0A during nutrient starvation (PubMed:12817086). Repressed by AbrB during regular growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Cell membrane
messages: Membrane
messages: Required for the maturation of SdpC to SDP (PubMed:12817086). Not required for SdpC signal peptide cleavage, secretion from the cell or disulfide bond formation (PubMed:23687264).
messages: When the sdpA-sdpB-sdpC operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086). A single deletion mutant does not have SDP activity (active peptide of sdpC) (PubMed:23687264).

224308:BSU 38480
gene id: 224308:BSU_38480
uniprot id: P39583
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the RelA/SpoT family.
messages: Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). Overexpression in relA mutants (triple relA-yjbM-ywaC deletions and single relA deletions) leads to growth arrest; GTP levels fall drastically, various guanine-related nucleotides are synthesized (ppGp or pGpp), the cellular transcriptional profile changes dramatically and 70S ribosome dimerization occurs (PubMed:22950019). Overexpression in the presence of a wild-type relA gene does not have these effects (PubMed:22950019). In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response (PubMed:18067544).
messages: Homotetramer.
messages: No visible phenotype, double yjbM-ywaC and triple relA-yjbM-ywaC mutants are also viable (PubMed:18067544).
messages: The synthase activity of YwaC is weaker than that of YjbM (PubMed:18067544).
messages: Weakly expressed during exponential growth with a marked and transient increase at the onset of the stationary phase (PubMed:18067544). Protein expression is very low during the first 3.5 hours after inoculation (at protein level) (PubMed:22950019). Induced by alkaline shock (PubMed:18067544).

224308:BSU 07360
gene id: 224308:BSU_07360
uniprot id: O06477
names: Function
names: Induction
names: Similarity
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation (By similarity).
messages: Transcriptionally regulated by SigD; part of the yfmT/yfmS operon.

224308:BSU 17570
gene id: 224308:BSU_17570
uniprot id: A0A6M4JGN7
uniprot id: P94488
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:galactoside symporter (TC 2.A.2) family.
messages: Cell membrane
messages: Membrane

224308:BSU 02990
gene id: 224308:BSU_02990
uniprot id: A0A6M3Z731
uniprot id: P46921
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: The complex is composed of two ATP-binding proteins (OpuAA), two transmembrane proteins (OpuAB) and a solute-binding protein (OpuAC).

224308:BSU 12920
gene id: 224308:BSU_12920
uniprot id: P26902
names: Cofactor
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the peptidase M55 family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Expressed early during sporulation.
messages: Homodecamer. A 20 Angstroms wide channel runs through the complex, giving access to a central chamber holding the active sites.
messages: Hydrolyzes N-terminal residues in D-amino acid containing peptides. Among the tested substrates, the highest activities are with D-Ala-D-Ala and D-Ala-Gly-Gly. The physiological role is not clear.
messages: Nutrient deficiency conditions, which also induce sporulation.

224308:BSU 26820
gene id: 224308:BSU_26820
uniprot id: O05411
names: Similarity
messages: To B.subtilis YncM.

224308:BSU 17070
gene id: 224308:BSU_17070
uniprot id: O31780
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 14540
gene id: 224308:BSU_14540
uniprot id: A0A6M3ZAJ5
uniprot id: O31718
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A non-essential component of RNA polymerase (RNAP) (PubMed:6802805, PubMed:18289874, PubMed:21710567, PubMed:25092033). Has a similar structure to bacteriophage T7 protein Gp2 (AC P03704), which is known to bind to RNAP in the DNA binding-cleft. Unlike Gp2 however, this protein does not inhibit transcription initiation (PubMed:25092033). In vitro reconstitution experiments show this subunit is dispensible (PubMed:18289874).
messages: A non-essential component of RNA polymerase (RNAP).
messages: Belongs to the RNA polymerase subunit epsilon family.
messages: Monomer (By similarity). RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567, PubMed:25092033). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: No visible phenotype; growth, bacterial fitness in competition experiments, RNAP distribution, global transcript levels and transcription initiation in vitro are all unaffected.
messages: Part of the rpoY-rnjA operon, transcribed constitutively.
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
messages: nucleoid

224308:BSU 27420
gene id: 224308:BSU_27420
uniprot id: A0A6M3ZF50
uniprot id: O34472
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.
messages: Cell membrane
messages: Membrane

224308:BSU 40480
gene id: 224308:BSU_40480
uniprot id: A0A6M4JR46
uniprot id: P37482
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family.
messages: Cell membrane
messages: Membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 38150
gene id: 224308:BSU_38150
uniprot id: A0A6M3ZLJ2
uniprot id: P34958
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome c oxidase subunit 3 family.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water. Major component for energy conversion during vegetative growth (By similarity).
messages: Cell membrane
messages: Membrane

224308:BSU 05220
gene id: 224308:BSU_05220
uniprot id: P96667
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 40880
gene id: 224308:BSU_40880
uniprot id: A0A6M3ZIG2
uniprot id: P37454
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DNA repair enzymes AP/ExoA family.
messages: Cytoplasm
messages: Probably binds two magnesium or manganese ions per subunit.

224308:BSU 10580
gene id: 224308:BSU_10580
uniprot id: A0A6M3Z9K3
uniprot id: O07569
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 15970
gene id: 224308:BSU_15970
uniprot id: A0A6M3ZAM1
uniprot id: P37104
names: Function
names: Similarity
messages: Belongs to the UPF0122 family.
messages: Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein.

224308:BSU 05860
gene id: 224308:BSU_05860
uniprot id: O05510
names: Activity Regulation
names: Function
names: Induction
names: Similarity
messages: Belongs to the ROK (NagC/XylR) family.
messages: Inhibited by zinc ions.
messages: Seems to be involved in the degradation of glucomannan.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 18540
gene id: 224308:BSU_18540
uniprot id: A0A6M3ZBC7
uniprot id: O34864
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. CsbX subfamily.
messages: Cell membrane
messages: Induced by sulfate, part of the yoaDCB operon.
messages: Membrane

224308:BSU 25470
gene id: 224308:BSU_25470
uniprot id: A0A6M4JJA3
uniprot id: P17820
names: Function
names: Induction
names: Similarity
messages: Acts as a chaperone.
messages: Belongs to the heat shock protein 70 family.
messages: By heat shock.
messages: By stress conditions e.g. heat shock.

224308:BSU 05420
gene id: 224308:BSU_05420
uniprot id: A0A6M4JGS4
uniprot id: P96686
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YdfH/YdfI. Regulates the transcription of ydfJ by binding to its promoter region.
messages: Phosphorylated by YdfH.

224308:BSU 35990
gene id: 224308:BSU_35990
uniprot id: P80871
names: Induction
names: Similarity
messages: Belongs to the NAD(P)H dehydrogenase (quinone) family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 18840
gene id: 224308:BSU_18840
uniprot id: A0A6M4JHE2
uniprot id: P18429
names: Similarity
messages: Belongs to the glycosyl hydrolase 11 (cellulase G) family.

224308:BSU 31640
gene id: 224308:BSU_31640
uniprot id: A0A6M4JNA4
uniprot id: Q7WY60
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 36920
gene id: 224308:BSU_36920
uniprot id: A0A6M4JQ63
uniprot id: P39156
names: Induction
names: Similarity
messages: Belongs to the LacAB/RpiB family.
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 04280
gene id: 224308:BSU_04280
uniprot id: P96585
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 23370
gene id: 224308:BSU_23370
uniprot id: P31847
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane raft
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 16020
gene id: 224308:BSU_16020
uniprot id: A0A6M3ZBC8
uniprot id: O31740
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.
messages: Belongs to the RimM family.
messages: Binds ribosomal protein uS19.
messages: Cytoplasm
messages: The PRC barrel domain binds ribosomal protein uS19.

224308:BSU 01680
gene id: 224308:BSU_01680
uniprot id: A0A6M3Z7C4
uniprot id: Q797S1
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.

224308:BSU 11360
gene id: 224308:BSU_11360
uniprot id: A0A6M4JGZ5
uniprot id: P42064
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transporting tripeptides. Probably responsible for energy coupling to the transport system.

224308:BSU 40470
gene id: 224308:BSU_40470
uniprot id: P37481
names: Induction
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 22740
gene id: 224308:BSU_22740
uniprot id: A3F3D7
uniprot id: P31114
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the FPP/GGPP synthase family.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Heterodimer of component I and II.
messages: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7).

224308:BSU 37720
gene id: 224308:BSU_37720
uniprot id: P39640
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Cells lacking this gene show an accumulation of dihydroanticapsin and dihydrobacilysin.
messages: Part of the bacABCDEFG operon responsible for the biosynthesis of bacilysin, an irreversible inactivator of the glutaminase domain of glucosamine synthetase. Catalyzes the dehydrogenation of the C7-hydroxyl group in the 4S-tetrahydrotyrosine (4S-H4Tyr) to yield anticapsin (epoxycyclohexanonyl-Ala). It is not able to oxidize the 4R-H4Tyr diastereomer and the dihydrobacilysin dipeptide (L-Ala-4S-H4Tyr dipeptide).
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDE operon and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 24210
gene id: 224308:BSU_24210
uniprot id: P54524
names: Similarity
messages: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

224308:BSU 04540
gene id: 224308:BSU_04540
uniprot id: A0A6M3Z7K8
uniprot id: P96610
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.
messages: Cell membrane
messages: Membrane

224308:BSU 38770
gene id: 224308:BSU_38770
uniprot id: A0A6M3ZHQ2
uniprot id: P94363
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:citrate (SCF) symporter family.
messages: Cell membrane
messages: Membrane
messages: Proton motive force-driven secondary transporter that catalyzes the uptake of both citrate and malate. Appears to be an electroneutral proton-solute symporter; the number of protons transported is equal to the valence of the transported anions. Is strictly stereoselective, translocating only the (S)-enantiomer of malate.
messages: The uptake activity is inhibited by divalent metal ions such as Ca(2+), Mg(2+) and Ni(2+).

224308:BSU 27729
gene id: 224308:BSU_27729
uniprot id: C0H463
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 15150
gene id: 224308:BSU_15150
uniprot id: A0A6M3ZAE4
uniprot id: Q07867
names: Caution
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsL family.
messages: Cell membrane
messages: Cleaved by RasP. Cleavage is important for turnover and function of FtsL.
messages: Essential cell division protein that may play a structural role. Probably involved in the regulation of the timing of cell division. Also required for sporulation.
messages: Essential cell division protein.
messages: Essential, it cannot be deleted.
messages: Is thermodynamically highly unstable and is likely to be rapidly degraded unless stabilized by interaction with other septasomal proteins.
messages: Membrane
messages: Monomer. Interacts with DivIB and DivIC. Interaction with DivIC stabilizes FtsL against RasP cleavage.
messages: The cytoplasmic region is involved in protein stability.
messages: The question of whether FtsL and DivIC interact directly (PubMed:16936019) or indirectly (PubMed:11994149) remains controversial.
messages: Transcribed at a low constant level in all growth phases. Part of the mraZ-rsmH-ftsL-pbpB operon.

224308:BSU 12740
gene id: 224308:BSU_12740
uniprot id: P54340
names: Similarity
messages: To B.subtilis YqcA.

224308:BSU 31470
gene id: 224308:BSU_31470
uniprot id: O05231
names: Cofactor
names: Function
messages: Binds 2 magnesium ions per subunit.
messages: Specifically inhibits the KinA pathway to sporulation.

224308:BSU 02010
gene id: 224308:BSU_02010
uniprot id: O31433
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YbdK/YbdJ. Probably activates YbdJ by phosphorylation.

224308:BSU 40530
gene id: 224308:BSU_40530
uniprot id: A0A6M4JR63
uniprot id: P23261
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Generates the two 5 and 8 kDa components of the spore coat, after proteolytic processing.
messages: Present in an increased level in yabG mutant spores.
messages: Spore coat

224308:BSU 22090
gene id: 224308:BSU_22090
uniprot id: A0A6M3ZGL1
uniprot id: P50847
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the KdgT transporter family.
messages: Catalyzes the proton-dependent uptake of 2-keto-3-deoxygluconate (KDG) into the cell.
messages: Cell membrane
messages: Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.

224308:BSU 30850
gene id: 224308:BSU_30850
uniprot id: A0A6M3ZFW4
uniprot id: P42407
names: Similarity
messages: Belongs to the UDPGP type 2 family.

224308:BSU 34600
gene id: 224308:BSU_34600
uniprot id: A0A6M4JPH7
uniprot id: O06990
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Probably responsible for the translocation of the substrate across the membrane.
messages: Part of the ABC transporter complex involved in maltodextrin import. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MdxF and MdxG) and a solute-binding protein (MdxE).

224308:BSU 15190
gene id: 224308:BSU_15190
uniprot id: A0A6M4JMT9
uniprot id: Q03521
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 4 family. MraY subfamily.
messages: Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.
messages: Cell membrane
messages: Membrane

224308:BSU 27360
gene id: 224308:BSU_27360
uniprot id: A0A6M3ZED8
uniprot id: O32036
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.
messages: Binds magnesium, but the ion is dispensable for mo5U forming activity.
messages: Catalyzes the methylation of 5-hydroxyuridine (ho5U) to form 5-methoxyuridine (mo5U) at position 34 in tRNAs.
messages: Deletion mutant does not contain mo5U.
messages: Homodimer.

224308:BSU 17200
gene id: 224308:BSU_17200
uniprot id: A0A6M4JIW7
uniprot id: P40872
names: Cofactor
names: Function
names: Subcellular Location Annotation
messages: Binds 4 phosphopantetheines covalently.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 35470
gene id: 224308:BSU_35470
uniprot id: P39145
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the helicase family.
messages: Cytoplasm
messages: Destabilization of ComGA (PubMed:17630974).
messages: Expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:11918817, PubMed:16009133, PubMed:17630974).
messages: Expression activated by ComK (PubMed:11918817, PubMed:11948146).
messages: Involved in transformation (competence for DNA uptake). Required for DNA uptake but not for binding. May provide the driving force for transport of DNA through an aqueous channel.

224308:BSU 11650
gene id: 224308:BSU_11650
uniprot id: A0A6M4JF32
uniprot id: P25052
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the TenA family.
messages: Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway.
messages: Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway. Thus, catalyzes the conversion of 4-amino-5-aminomethyl-2-methylpyrimidine to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). To a lesser extent, is also able to catalyze the hydrolytic cleavage of thiamine; however, this thiaminase activity is unlikely to be physiologically relevant. Therefore, is involved in the regeneration of the thiamine pyrimidine from thiamine degraded products present in the environment, rather than in thiamine degradation.
messages: Homotetramer.
messages: Inactivation of this gene causes a delay in sporulation, but does not affect cell growth and the production of extracellular enzymes (PubMed:1898926). The deletion mutant does not require a hydroxypyrimidine source as it is able to biosynthesize it using ThiA; the tenA/thiA double mutant, however, is hydroxypyrimidine-requiring and is unable to salvage the pyrimidine from formylaminopyrimidine, aminopyrimidine, or base-degraded thiamine (PubMed:17618314).
messages: Strongly repressed by thiamine.
messages: Was originally described as a regulatory protein involved in the regulation of the production of extracellular enzymes.

224308:BSU 27220
gene id: 224308:BSU_27220
uniprot id: A0A6M4JRM0
uniprot id: Q795Y4
names: Cofactor
names: Similarity
messages: Binds 1 [2Fe-2S] cluster.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
messages: Binds 4 [4Fe-4S] clusters.
messages: In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

224308:BSU 30050
gene id: 224308:BSU_30050
uniprot id: A0A6M4JL03
uniprot id: O34674
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide synthase family.
messages: Cell membrane
messages: Cells lacking this gene are viable, have no defect in growth, but produce fewer spores, slightly longer cells and show an increase in cell chain formation. Also exhibits some resistance to moenomycin (PubMed:19648239, PubMed:19666716). Mutants lacking both murJ and amj have a lethal defect in cell wall synthesis (PubMed:25918422).
messages: Expressed at low levels during vegetative growth. Expression decreases significantly during sporulation.
messages: Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane (PubMed:25918422). Not essential for growth (PubMed:19648239, PubMed:19666716).
messages: Membrane

224308:BSU 34080
gene id: 224308:BSU_34080
uniprot id: A0A6M4JPC3
uniprot id: O07017
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC-2 integral membrane protein family.
messages: Cell membrane
messages: Membrane

224308:BSU 03789
gene id: 224308:BSU_03789
uniprot id: A0A6M4JEY5
uniprot id: C0H3V1
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Membrane

224308:BSU 09950
gene id: 224308:BSU_09950
uniprot id: A0A6M4JGI4
uniprot id: P24327
names: Domain
names: Function
names: Sequence Caution
names: Similarity
names: Subcellular Location Annotation
messages: All three domains (PPIase domain and the flanking N- and C-terminal domains) are essential for activity.
messages: Belongs to the PrsA family.
messages: Cell membrane
messages: Essential protein that plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Has PPIase activity but it is not essential for its function in vivo.
messages: Membrane
messages: Membrane raft
messages: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins.
messages: Sequencing errors. The N-terminus of this sequence is probably the N-terminus of phrI.

224308:BSU 11020
gene id: 224308:BSU_11020
uniprot id: A0A6M4JLW7
uniprot id: O06746
names: Similarity
messages: Belongs to the UPF0234 family.

224308:BSU 16550
gene id: 224308:BSU_16550
uniprot id: A0A6M3ZB65
uniprot id: O31753
names: Function
names: Similarity
messages: Belongs to the DXR family.
messages: Catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).

224308:BSU 18990
gene id: 224308:BSU_18990
uniprot id: O34596
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the yobL-yobK operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; not dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YobL (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YobL upon expression in E.coli. Does not have immunity protein activity on other LXG toxins (PubMed:22200572).
messages: Interacts with cognate toxin YobL but not with non-cognate putative toxin YeeF. The interaction inhibits the toxic activity of YobL.

224308:BSU 33020
gene id: 224308:BSU_33020
uniprot id: A0A6M3ZGS0
uniprot id: O32193
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by phosphorylation.
messages: Membrane

224308:BSU 37920
gene id: 224308:BSU_37920
uniprot id: A0A6M3ZHV6
uniprot id: P39620
names: Developmental Stage
names: Function
names: Induction
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Essential for the localization of CwlJ in the spore coat and for spore germination triggered by calcium and dipicolinic acid (DPA). Its assembly into the spore coat is dependent on the coat morphogenetic proteins CotE and SpoIVA.
messages: Expressed during sporulation in mother cell compartment.
messages: Expression is sigma E-dependent.
messages: Multimer. Is cross-linked by Tgl and YabG into an insoluble high-molecular-mass complex that appears very late in sporulation.
messages: Spore coat
messages: Three N-terminal lysines form epsilon-(gamma-glutamyl)lysine isopeptide bonds with glutamines of other spore coat proteins.

224308:BSU 24300
gene id: 224308:BSU_24300
uniprot id: A0A6M3ZIY8
uniprot id: P54521
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the XseA family.
messages: Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
messages: Cytoplasm
messages: Heterooligomer composed of large and small subunits.

224308:BSU 07130
gene id: 224308:BSU_07130
uniprot id: A0A6M4JJD7
uniprot id: P39130
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Alpha-galacturonidase able to catalyze the hydrolysis of the chromogenic substrate p-nitrophenyl-alpha-D-galacturonic acid (pNPalphaGalUA), and of the probable natural substrate alpha-1,4-di-galacturonate (GalUA(2)). Can neither hydrolyze pNPbetaGalUA, nor the stereoisomeric pNPalphaGlcUA. Does not display alpha- or beta-glucosidase activity as it fails to hydrolyze melibiose, raffinose, lactose and the chromogenic analogs, pNPalphaGal and pNPbetaGal. Cannot use the following compounds as substrates: pNP-N-acetyl-alpha- and beta-D-galactosaminide, pNP-N-acetyl-alpha- and beta-D-glucosaminide, pNP-alpha-L- and beta-L-arabinopyranoside, pNP-alpha- and beta-D-glucuronide, pNP-alpha- and beta-D-glucopyranoside, pNP-alpha- and beta-D-glucopyranoside 6-phosphate, pNP-alpha-D-galactopyranoside 6-phosphate and oNP-beta-D-galactopyranoside 6-phosphate.
messages: Belongs to the glycosyl hydrolase 4 family.
messages: Binds 1 Mn(2+) ion per subunit. Although a Mg(2+) ion is seen in the structure, galacturonidase activity was shown using manganese (PubMed:23416295).
messages: Binds 1 NAD(+) per subunit.
messages: Homotetramer.

224308:BSU 23970
gene id: 224308:BSU_23970
uniprot id: A0A6M4JKJ4
uniprot id: P54536
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.
messages: Cell membrane
messages: Cells show impaired growth on arginine as the nitrogen source.
messages: Membrane
messages: Part of a binding-protein-dependent transport system for arginine. Probably responsible for the translocation of the substrate across the membrane.

224308:BSU 38090
gene id: 224308:BSU_38090
uniprot id: P29141
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Activity is inhibited by phenylmethylsulfonyl fluoride (PMSF), but not by EDTA.
messages: Belongs to the peptidase S8 family.
messages: Expression is negatively controlled by CodY, which binds to the regulatory regions of vpr, in the vicinity of its transcription start point (PubMed:25666135). Expression is sigma H-dependent (PubMed:25666135).
messages: Mutant lacking this gene does not show significant decrease in CSF production, but the triple deletion mutant aprE-epr-vpr is defective in the cleavage event to release mature CSF.
messages: Probably undergoes C-terminal processing or proteolysis (PubMed:1938892). Auto-processed to form active enzymes of several different molecular weights (PubMed:15596354).
messages: Secreted
messages: Serine protease (PubMed:1938892, PubMed:17666034). Involved in the production of the competence and sporulation stimulating factor CSF (PubMed:17666034). Is directly involved in the processing of pro-CSF to CSF (PubMed:17666034). Can also cleave pro-PhrA to PhrA, but cannot cleave pro-PhrE (PubMed:17666034). Shows fibrinolytic activity in vitro (PubMed:15596354). Not essential for growth or sporulation (PubMed:1938892).
messages: cell wall

224308:BSU 09270
gene id: 224308:BSU_09270
uniprot id: A0A6M4JEG1
uniprot id: P30300
names: Disruption Phenotype
names: Function
names: Induction
messages: By glycerol 3-phosphate. Repressed by glucose, glucose 6-phosphate and fructose 6-phosphate.
messages: Defective in glycerol 3-phosphate uptake. Noninducible for glpK and glpD. Is able to grow at concentrations of the antibiotic fosfomycin that is not tolerated by the wild-type.
messages: Regulates expression of the glpD operon. In the presence of glycerol 3-phosphate (G3P) causes antitermination of transcription of glpD at the inverted repeat of the leader region to enhance its transcription. Binds and stabilizes glpD leader mRNA.
messages: Regulates expression of the glpD operon. In the presence of glycerol 3-phosphate (G3P) causes antitermination of transcription of glpD at the inverted repeat of the leader region to enhance its transcription. Binds and stabilizes glpD leader mRNA. May also regulate expression of the glpFK operon.

224308:BSU 31710
gene id: 224308:BSU_31710
uniprot id: P33690
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FPP/GGPP synthase family.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Cell membrane
messages: Disruption of the gene prevents the development of competence as well as the transcription of comG, a late competence operon (PubMed:1715859). Disruption also decreases the expression of srfA, a regulatory operon needed for the expression of competence (PubMed:1715859).
messages: Expression is maximal early in growth and declines as the cells approach the stationary phase.
messages: Part of a major quorum-sensing system that regulates the development of genetic competence (PubMed:1715859, PubMed:11751817). Involved in the maturation of the competence pheromone ComX (PubMed:11751817, PubMed:31670609). Acts by catalyzing the transfer of a farnesyl group on the ComX pheromone (PubMed:31670609). Shows weak geranylation activity with geranyl diphosphate (GPP) (PubMed:31670609).
messages: The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.

224308:BSU 05830
gene id: 224308:BSU_05830
uniprot id: A0A6M4JJ13
uniprot id: O05507
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II GmuABC PTS system is involved in the transport of oligo-glucomannans such as cellobiose or mannobiose.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 12020
gene id: 224308:BSU_12020
uniprot id: A0A6M4JKM2
uniprot id: O31646
names: Cofactor
names: Disruption Phenotype
names: Induction
names: Similarity
messages: Belongs to the mannose-6-phosphate isomerase type 1 family.
messages: Binds 1 zinc ion per subunit.
messages: Cells lacking this gene are unable to grow in minimal medium with mannose as the sole carbon source. They show impaired growth in rich medium, and impairment increases in the presence of greater than 1.4 mM mannose.
messages: Up-regulated by mannose. Is under the control of ManR. Is subject to carbon catabolite repression (CCR) by glucose. Forms part of an operon with manP and yjdF.

224308:BSU 10180
gene id: 224308:BSU_10180
uniprot id: O07601
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38270
gene id: 224308:BSU_38270
uniprot id: A0A6M4JQI9
uniprot id: P39596
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the EfeM/EfeO family.
messages: Cell membrane
messages: Component of the iron transporter efeUOB/M complex composed of EfeU, EfeM and EfeB; EfeU is essential for the complex formation.
messages: Membrane raft
messages: Part of the iron transporter system efeUOB/M involved in iron import (PubMed:16672620, PubMed:23764491). Specifically binds Fe(3+), which is produced by EfeB-mediated oxidation of Fe(2+), and delivers it to the cell membrane permease EfeU (PubMed:23764491).
messages: Severe reduction in Fe(2+) and Fe(3+) uptake and in cell growth.
messages: The peptidase M75 domain contains 2 metal binding sites, site III and site IV (By similarity). Lacks the N-terminal Cup-like domain present in EfeO proteins that contains metal binding sites I and II (By similarity).

224308:BSU 01860
gene id: 224308:BSU_01860
uniprot id: A0A6M3ZA07
uniprot id: O34823
names: Caution
names: Similarity
messages: Belongs to the beta-class carbonic anhydrase family.
messages: This protein does not seem to be able to bind zinc, which all carbonic anhydrases are thought to require.

224308:BSU 16390
gene id: 224308:BSU_16390
uniprot id: A0A6M4JGR4
uniprot id: P35620
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.
messages: Cell membrane
messages: Involved in the export of flagellum proteins.
messages: Membrane
messages: Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.

224308:BSU 11710
gene id: 224308:BSU_11710
uniprot id: A0A6M4JIQ6
uniprot id: O31620
names: Function
names: Similarity
messages: Belongs to the ThiD family.
messages: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine.

224308:BSU 11400
gene id: 224308:BSU_11400
uniprot id: A0A6M4JGW2
uniprot id: P42063
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.
messages: Cell membrane
messages: Membrane
messages: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transporting tripeptides. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 00900
gene id: 224308:BSU_00900
uniprot id: A0A6M4JHP8
uniprot id: Q06755
names: Function
names: Similarity
messages: Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
messages: Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).

224308:BSU 30070
gene id: 224308:BSU_30070
uniprot id: A0A6M4JNY4
uniprot id: P54417
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Activity is stimulated by high osmolarity.
messages: Belongs to the BCCT transporter (TC 2.A.15) family.
messages: Cell membrane
messages: High-affinity uptake of glycine betaine. Does not mediate either carnitine or choline uptake.
messages: Induced by high osmolarity.
messages: Membrane

224308:BSU 06280
gene id: 224308:BSU_06280
uniprot id: O34592
names: Induction
names: Similarity
messages: Belongs to the AB hydrolase superfamily.
messages: Expressed under the control of the sigma-W factor SigW.

224308:BSU 09090
gene id: 224308:BSU_09090
uniprot id: A0A6M3ZE32
uniprot id: P54593
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 22120
gene id: 224308:BSU_22120
uniprot id: P50844
names: Function
names: Induction
messages: Induced by galacturonate and negatively regulated by itself. Is subject to catabolite repression by glucose involving the ccpA gene.
messages: Transcriptional repressor of the kdgRKAT and kduID operons for pectin utilization.

224308:BSU 28650
gene id: 224308:BSU_28650
uniprot id: P94538
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.
messages: Contains an extended N-terminal domain, which probably binds RNA, connected by an interdomain linker to a C-terminal catalytic domain, which adopts a typical SPOUT fold.
messages: Cytoplasm
messages: Homodimer.
messages: Mutant strain lacks G2553 methylation in 23S rRNA (PubMed:35710145). Loss of the gene does not affect growth nor lead to ribosome assembly intermediates accumulation (PubMed:35710145).
messages: Specifically methylates the ribose of guanosine 2553 (G2553) in 23S rRNA (PubMed:35710145). When the target G2553 is mutated, is able to methylate the ribose of adenosine, but it cannot methylate cytidine nor uridine (PubMed:35710145). Modifies free 23S rRNA but not the fully assembled ribosome nor the 50S subunit, suggesting that the modification occurs early during ribosome biogenesis (PubMed:35710145).

224308:BSU 02690
gene id: 224308:BSU_02690
uniprot id: A0A6M3Z701
uniprot id: O34482
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: B.subtilis contains two L-asparaginase isoenzymes: L-asparaginase I, a low-affinity enzyme located in the cytoplasm, and L-asparaginase II, a high-affinity secreted enzyme.
messages: Belongs to the asparaginase 1 family.
messages: Catalyzes the conversion of L-asparagine to L-aspartate and ammonium.
messages: Expression is induced during limiting-nitrogen conditions by the nitrogen regulatory factor TnrA.
messages: Homotetramer.

224308:BSU 01000
gene id: 224308:BSU_01000
uniprot id: A0A6M3Z6K7
uniprot id: Q06799
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SecE/SEC61-gamma family.
messages: Cell membrane
messages: Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.
messages: Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.

224308:BSU 30280
gene id: 224308:BSU_30280
uniprot id: A0A6M4JL23
uniprot id: O34706
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Deletion of the gene abolishes induction in the presence of melibiose and raffinose.
messages: Membrane
messages: Part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import. Probably responsible for the translocation of the substrate across the membrane.
messages: Repressed by the transcriptional regulator MelR. Induced by melibiose and raffinose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MelC and MelD) and a solute-binding protein (MelE).

224308:BSU 25870
gene id: 224308:BSU_25870
uniprot id: P45941
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the yqcF-yqcG operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YqcG (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YqcG upon expression in E.coli. Does not have immunity protein activity on other LXG toxins (PubMed:22200572).
messages: Probably interacts with cognate toxin YqcG but not with other non-cognate toxins. The interaction inhibits the toxic activity of YqcG (Probable).

224308:BSU 01080
gene id: 224308:BSU_01080
uniprot id: A0A6M3Z6P2
uniprot id: P37871
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RNA polymerase beta' chain family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 2 Zn(2+) ions per subunit.
messages: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

224308:BSU 04510
gene id: 224308:BSU_04510
uniprot id: P96607
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 35090
gene id: 224308:BSU_35090
uniprot id: A0A6M3ZH33
uniprot id: O34307
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane

224308:BSU 28220
gene id: 224308:BSU_28220
uniprot id: A0A6M4JKG0
uniprot id: P50866
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity). Probably the major protease that degrades proteins tagged by trans-translation (PubMed:11395451).
messages: ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.
messages: Belongs to the ClpX chaperone family.
messages: By heat shock.
messages: Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.
messages: No degradation of trans-translationally tagged-peptides.

224308:BSU 13490
gene id: 224308:BSU_13490
uniprot id: A0A6M4JHX7
uniprot id: O31657
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M48B family.
messages: Binds 1 zinc ion per subunit.
messages: Cell membrane
messages: Membrane

224308:BSU 15650
gene id: 224308:BSU_15650
uniprot id: O34431
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
messages: Cell membrane
messages: Expressed from 4 hours and peaks at 9 hours after onset of sporulation.
messages: Expressed under control of sigma-F (sigF).
messages: Phosphorylated in a Ca(2+)-dependent manner starting 4 hours after shifting to sporulation medium.
messages: Spores are less resistant to heat and germinate at a slower rate, Ca(2+)-dependent phosphorylation is no longer seen.
messages: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.

224308:BSU 21040
gene id: 224308:BSU_21040
uniprot id: O31945
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: A single subunit DNA-dependent RNA polymerase (RNAP) that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates (rNTPs) as substrates. The enzyme is more highly processive than the multisubunit RNAP from E.coli but is considerably more error-prone. It has no detectable proof-reading function but can perform pyrophosphorolysis. Transcribes the late genes of the SPbeta prophage starting from yonK (approximately 35 genes are encoded in the prophage downstream from yonK).
messages: Belongs to the YRH RNA polymerase family.
messages: Encoded by the SPbeta prophage.
messages: No expression under phage non-inducing conditions; induced by mitomycin C (at protein level).
messages: No longer forms lytic phage.
messages: Uses Mg(2+) and Mn(2+) equivalently.

224308:BSU 08980
gene id: 224308:BSU_08980
uniprot id: A0A6M3ZE20
uniprot id: P45742
names: Induction
names: Similarity
messages: Belongs to the UPF0229 family.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.

224308:BSU 37050
gene id: 224308:BSU_37050
uniprot id: A0A6M4JQ58
uniprot id: P45868
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the malic enzymes family.
messages: Catalyzes the decarboxylation of malate to pyruvate (PubMed:12949160, PubMed:16788182). Can use NAD and NADP, but with a strong preference for NAD (PubMed:12949160, PubMed:16788182). Can also catalyze the decarboxylation of oxaloacetate (PubMed:16788182). Involved in keeping the ATP levels high (PubMed:23136871).
messages: Divalent metal cations.
messages: Divalent metal cations. Prefers magnesium or manganese.
messages: Induced in the presence of malate via the two-component system MalK/MalR. The regulator MalR binds to the promoter region of maeA. Is not subject to carbon catabolite repression.
messages: Mutant can use malate, succinate plus glutamate or glucose as efficiently as the wild-type strain (PubMed:12949160, PubMed:16788182). The ATP concentrations in the mutant grown in minimal medium with glucose are similar to the wild-type level. ATP concentrations decrease by about 10% in malate minimal medium. The mleA-maeA-malS triple mutant shows a decrease in ATP concentrations by about 20% and a moderate growth defect (PubMed:23136871). NADPH overproduction is roughly halved in the deletion mutant (PubMed:33824210).

224308:BSU 35370
gene id: 224308:BSU_35370
uniprot id: A0A6M4JPP1
uniprot id: P33911
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Usually binds in the 5'-UTR at or near the Shine-Dalgarno sequence preventing ribosome-binding, thus repressing translation. Its main target seems to be the major flagellin gene, while its function is anatagonized by FliW.
messages: A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Usually binds in the 5'-UTR at or near the Shine-Dalgarno sequence preventing ribosome-binding, thus repressing translation. Represses expression of flagellin (hag) in a post-transcriptional fashion. Specifically binds to 2 sites in the 5'-UTR of hag mRNA in a cooperative fashion; the second site overlaps the Shine-Dalgarno sequence and prevents 30S ribosomal subunit binding (PubMed:17555441). Mutation of either binding site abolishes CsrA regulation of hag expression (PubMed:17555441, PubMed:21895793). Repression is greater in the 1A96 than 168 genetic background and higher in minimal than rich medium (PubMed:17555441). Translation repression is antagonized by FliW (PubMed:21895793). Partner switching by flagellin between FliW and CsrA provides a flagellar assembly checkpoint to tightly control the timing of flagellin synthesis. Flagellin binds to assembly factor FliW, freeing CsrA to repress translation of the flagellin mRNA. When the flagellar hook is assembled flagellin is secreted, depleting intracellular flagellin, which frees FliW to interact with CsrA and inhibits CsrA binding to mRNA. This derepresses flagellin translation and provides protein for flagellar assembly. Once the flagellar filament is completed cytoplasmic flagellin levels rise and CsrA translation repression of flagellin reinitiates (PubMed:21895793, PubMed:27516547). Overexpression leads to a dramatic reduction in motility, a significant reduction in flagellin synthesis and reduced flagella assembly (PubMed:21895793).
messages: Belongs to the CsrA/RsmA family.
messages: Contacts FliW via its C-terminus (residues 49-58).
messages: Cytoplasm
messages: Homodimer (PubMed:17555441). The beta-strands of each monomer intercalate to form a hydrophobic core while the alpha-helices form wings that extend away from the core (By similarity). Two molecules of FliW interact with 1 homodimer (PubMed:21895793, PubMed:27516547). mRNA and FliW bind to different sites on CsrA (PubMed:27516547).
messages: Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core, while the alpha-helices form wings that extend away from the core.
messages: Increased expression of flagellin (hag), 30% decrease in motility halo (PubMed:17555441). Suppresses the motility loss and flagellar assembly defect of an fliW deletion (PubMed:21895793). Suppresses the phenotypes associated with deletion of the intracellular flagella chaperone fliS (PubMed:23144244).
messages: Part of the SigD-controlled yvyF-csrA operon and the SigA-controlled fliW-csrA operon (PubMed:17555441). Expressed from the SigA operon at low levels during log phase, with a 5-fold increase as the culture transitions to stationary phase, peaking 1 hour later.

224308:BSU 09750
gene id: 224308:BSU_09750
uniprot id: A0A6M4JGG1
uniprot id: P04832
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the alpha/beta-type SASP family.
messages: SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.
messages: SASP are degraded in the first minutes of spore germination and provide amino acids for both new protein synthesis and metabolism.

224308:BSU 07140
gene id: 224308:BSU_07140
uniprot id: A0A6M3ZBK7
uniprot id: O31533
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0702 family.
messages: Cell membrane
messages: Membrane

224308:BSU 01440
gene id: 224308:BSU_01440
uniprot id: A0A6M4JE82
uniprot id: P20277
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL17 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Contacts protein L32 (By similarity).
messages: Part of the 50S ribosomal subunit. Contacts protein L32.

224308:BSU 21680
gene id: 224308:BSU_21680
uniprot id: A0A6M4JIG1
uniprot id: P54155
names: Caution
names: Similarity
messages: Belongs to the MsrB Met sulfoxide reductase family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 32050
gene id: 224308:BSU_32050
uniprot id: A0A6M3ZKL3
uniprot id: O32106
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M17 family.
messages: Binds 2 manganese ions per subunit.
messages: Cytoplasm
messages: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).
messages: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

224308:BSU 09990
gene id: 224308:BSU_09990
uniprot id: A0A6M3Z9K4
uniprot id: P11065
names: Function
names: Induction
names: Subunit
messages: Homodimer.
messages: Homodimer. Interacts with SinR.
messages: Negative regulator of protease production and sporulation.
messages: Negative regulator of protease production and sporulation. Acts by binding directly to the promoter of protease genes (aprE and nprE), and by repressing oligopeptide permease operons (appABCDF and oppABCDF), thereby preventing uptake of oligopeptides required for initiation of sporulation. Acts with SinR as a corepressor of epr expression. Binds to non-m6A-5-methylated 5'-GACGAG-3' sites, tested with scpA; when the target is methylated by DnmA, this repressor no longer binds and transcription is up-regulated (PubMed:32324221).
messages: Negatively regulated by the Mrp homolog protein SalA and by SenS.

224308:BSU 00490
gene id: 224308:BSU_00490
uniprot id: A0A6M3Z6E4
uniprot id: P28015
names: Developmental Stage
names: Function
names: Similarity
messages: Belongs to the SpoVG family.
messages: Essential for sporulation. Interferes with or is a negative regulator of the pathway leading to asymmetric septation.
messages: Specific to stage V sporulation.

224308:BSU 40350
gene id: 224308:BSU_40350
uniprot id: P38022
names: Disruption Phenotype
names: Function
names: Induction
messages: Cells lacking this gene do not grow in presence of arginine as sole nitrogen source.
messages: Not induced by arginine, repressed by RocR itself.
messages: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences.

224308:BSU 10630
gene id: 224308:BSU_10630
uniprot id: P23478
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: An essential component of the DNA double-stranded break repair machinery, the heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' -> 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddA nuclease domain in particular is required for chi fragment generation; this subunit has 3' -> 5' nuclease and helicase activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.
messages: At low magnesium concentrations there is no nuclease activity, but helicase activity is unaffected.
messages: Belongs to the helicase family. AddA subfamily.
messages: Heterodimer of AddA and AddB.
messages: This enzyme is a functional homolog of the E.coli RecBCD enzyme; unlike the RecBCD enzyme it degrades both duplex strands symmetrically.

224308:BSU 08130
gene id: 224308:BSU_08130
uniprot id: O31554
names: Similarity
names: Subcellular Location Annotation
messages: Membrane
messages: To B.subtilis YfjD.

224308:BSU 03810
gene id: 224308:BSU_03810
uniprot id: A0A6M3Z7N3
uniprot id: P94419
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YclNOPQ involved in uptake of ferric-petrobactin. Petrobactin is a photoreactive 3,4-catecholate siderophore produced by many members of the B.cereus group, including B.anthracis. Probably responsible for the translocation of the substrate across the membrane.
messages: The complex is composed of two ATP-binding proteins (YclP), two transmembrane proteins (YclN and YclO) and a solute-binding protein (YclQ).

224308:BSU 09540
gene id: 224308:BSU_09540
uniprot id: A0A6M3Z9E0
uniprot id: O07584
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
messages: Cell membrane
messages: Cells lacking this gene cease to produce phospholipid and accumulate fatty acids arising from the dephosphorylation of 1-acylglycerol-3-P.
messages: Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) by incorporating an acyl moiety at the 2 position. This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.
messages: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

224308:BSU 36470
gene id: 224308:BSU_36470
uniprot id: A0A6M3ZLA5
uniprot id: P94575
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the purine-cytosine permease (2.A.39) family.
messages: Cell membrane
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or allantoate are added during limiting-nitrogen conditions.
messages: Membrane
messages: Uptake of allantoin into the cell (PubMed:11344136, PubMed:26967546). Allantoin uptake is not dependent on sodium, and PucI is likely to be a proton-coupled symporter (PubMed:26967546). Shows highest recognition for binding of allantoin, good recognition for binding of hydantoin, L-5-benzylhydantoin and 5-hydroxyhydantoin, and to a lesser extent for a range of nucleobases and nucleosides (PubMed:26967546).

224308:BSU 14040
gene id: 224308:BSU_14040
uniprot id: O34816
names: Developmental Stage
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the YkuD family.
messages: Expressed in the mother cell compartment from T4 of sporulation.
messages: Inactivation of the ykuD gene does not affect vegetative growth, spore resistance to heat, chloroform and lysozyme, or spore germination in the presence of L-alanine.
messages: LysM domains are thought to be involved in peptidoglycan binding.
messages: Monomer.
messages: Probable enzyme that may play an important role in cell wall biology.
messages: Regulated by SigK. Could also be negatively regulated by GerE. Transcribed by SigK RNA polymerase.
messages: Spore wall

224308:BSU 03820
gene id: 224308:BSU_03820
uniprot id: P94420
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Disruption mutants are unable to use petrobactin for iron delivery and growth.
messages: Part of the ABC transporter complex YclNOPQ involved in uptake of ferric-petrobactin. Petrobactin is a photoreactive 3,4-catecholate siderophore produced by many members of the B.cereus group, including B.anthracis. Probably responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (YclP), two transmembrane proteins (YclN and YclO) and a solute-binding protein (YclQ).

224308:BSU 04359
gene id: 224308:BSU_04359
uniprot id: C0H3V4
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 17660
gene id: 224308:BSU_17660
uniprot id: O31801
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the dUTPase family.
messages: Binds 1 Mg(2+) per subunit, coordinated entirely by the nucleotide and ordered water molecules.
messages: Homotrimer.
messages: Involved in nucleotide metabolism: produces dUMP, the immediate precursor of thymidine nucleotides and decreases the intracellular concentration of dUTP, so that uracil cannot be incorporated into DNA (Probable). The Ser-64 side chain changes its position upon ligand-binding to make contacts with the nucleotide phosphates (PubMed:20823546).
messages: No visible phenotype; double yncF-yosS deletions (both encode dUTPases) are also viable.
messages: The uracil deoxyribose moiety interacts with the protein through Ile-81 and Tyr-85, which discriminate against the ribose form of the nucleotide.

224308:BSU 19460
gene id: 224308:BSU_19460
uniprot id: O31857
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the PIGL family.
messages: Involved in bacillithiol (BSH) biosynthesis. Catalyzes the second step of the pathway, the deacetylation of N-acetylglucosaminylmalate (GlcNAc-Mal) to glucosamine malate (GlcN-Mal).
messages: Mutant has normal levels of bacillithiol, but bshB1/bshB2 double mutant does not produce bacillithiol.

224308:BSU 28940
gene id: 224308:BSU_28940
uniprot id: P94512
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily.
messages: Catalyzes the last step of the phosphorylated serine biosynthetic pathway, i.e. dephosphorylation of O-phospho-L-serine to form L-serine. To a lesser extent, is also able to dephosphorylate phosphothreonine, phosphoethanolamine, and histidinol phosphate in vitro.
messages: Cells lacking this gene cannot grow in minimal medium, in contrast to wild type. ysaA, serA, and serC deletion mutants have virtually identical profiles when observed across all the conditions studied, likely placing YsaA in the serine biosynthesis pathway. The defect is complemented by glycine, or a combination of serine and other amino acids such as glutamine or glutamate. The inability of serine alone to rescue the growth defect of the deletion mutant is due to serine toxicity.
messages: ysaA complements E.coli serB and vice versa.

224308:BSU 29450
gene id: 224308:BSU_29450
uniprot id: A0A6M4JN29
uniprot id: O34347
names: Caution
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the argininosuccinate synthase family. Type 1 subfamily.
messages: Cytoplasm
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 22630
gene id: 224308:BSU_22630
uniprot id: A3F3C6
uniprot id: P07601
names: Function
names: Similarity
names: Subunit
messages: Belongs to the TrpA family.
messages: Tetramer of two alpha and two beta chains.
messages: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

224308:BSU 14530
gene id: 224308:BSU_14530
uniprot id: A0A6M4JFW4
uniprot id: Q45493
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: 30S ribosomal subunit binding to Shine-Dalgarno sequences blocks exonuclease activity.
messages: An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.
messages: An RNase that has endonuclease and 5'-3' exonuclease activity, playing a role in both rRNA and mRNA stability and degradation. Endonuclease activity can cleave within 4 nucleotides of the 5'-end of a triphosphorylated RNA. Endonuclease digestion by the RNase J1/J2 complex occurs at a different site and in some cases more efficiently than J1 or J2 alone. The exonuclease activity of the J1/J2 complex is highly processive on substrates longer than 5 nucleotides, on shorter substrates is distributive. Preferentially cleaves ssRNA, possibly in AU-rich regions. The 5'-exonuclease activity acts on 5'-hydroxyl and 5'-monophosphate but not 5'-triphosphate ends; it can digest through stem-loop structures if they are not too stable. Required for maturation of 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal subunits. Plays a role in the secondary pathway of 23S rRNA 5' end maturation. Probably also participates in processing of pre-scRNA (the precursor of the signal recognition particle RNA). Major RNase that degrades both RNAs of the type I toxin-antitoxin system BsrE/SR5 (PubMed:26940229).
messages: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.
messages: Binds 1 Ca(2+) cation per subunit. Seen in 1 crystal structure, it is not clear if it is physiologically important.
messages: Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.
messages: Cytoplasm
messages: Essential. In depletion experiments there is decreased 5'-exonuclease processing of 16S rRNA (PubMed:17512403), decreased accumulation of correctly-sized scRNA (PubMed:17576666), decreased decay of trp mRNA leader (PubMed:18445592), while correct processing of the 5' end of 23S rRNA no longer occurs in the absence of mrnC (PubMed:19880604). While depletion/deletion of RNase J1 or J2 has no large impact on global gene expression, a double mutant alters the expression of hundreds of genes (PubMed:18713320). In a more severe depletion experiment alteration of about 30% of transcripts was seen (PubMed:22412379). Later shown not to be essential in 4 strains, with a tripled doubling time. 168 trpC2 cells able to grow on minimal medium. Loss of competence for plasmid transformation, nearly complete loss of sporulation, poor growth at 30 degrees Celsius and no growth under 25 degrees Celsius. Increased sensitivity to a wide range of antibiotics. Irregularly shaped cells form clumps of spiral cells connected by long chains, with few visible septa, cell walls are altered with looser, less dense peptidoglycan. Double pnp-rnjA or rnjA-rny mutants could not be isolated (PubMed:23504012). Increased half-life of both RNAs of the type I toxin-antitoxin system BsrE/SR5 (PubMed:26940229).
messages: Homodimer, may be a subunit of the RNA degradosome.
messages: Part of the rpoY-rnjA operon, transcribed constitutively.
messages: Present in about 2500 monomers per cell in mid-log phase.
messages: The C-terminal domain (residues 450-555) are required for nuclease activity and dimerization.
messages: Unclear whether it forms homodimers or belongs to a larger complex. According to (PubMed:20025672) probably does not form homodimers, while (PubMed:21893285) shows homodimer formation. Both reports show RNase J1 and J2 interaction, probably as a heterotetramer (PubMed:19193632) shows it is a component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno, while (PubMed:20025672) finds no evidence of an RNA degradosome complex.

224308:BSU 05950
gene id: 224308:BSU_05950
uniprot id: O05519
names: Similarity
messages: Belongs to the ABC transporter superfamily. ABCF family. YdiF subfamily.

224308:BSU 17220
gene id: 224308:BSU_17220
uniprot id: O31784
names: Cofactor
names: Function
names: Subcellular Location Annotation
messages: Binds 3 phosphopantetheines covalently.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 04270
gene id: 224308:BSU_04270
uniprot id: O31486
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 34160
gene id: 224308:BSU_34160
uniprot id: A0A6M4JPD8
uniprot id: O07009
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 1 family.
messages: Cell membrane
messages: Deletion mutant displays a 2-fold increase in the doubling time when galactan is the sole carbon and energy source.
messages: Involved in galactan degradation (PubMed:27501980, PubMed:29240795). Part of the ABC transporter complex GanPQS involved in the uptake of galactooligosaccharides (PubMed:27501980, PubMed:29240795). Binds mainly galactotetraose and galactotriose (PubMed:27501980).
messages: Repressed by the transcriptional regulator GanR and induced by galactobiose. Also repressed by glucose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (GanP and GanQ) and a solute-binding protein (GanS).
messages: Was originally described as a cyclodextrin-binding protein, but it was shown later that it has no affinity for cyclodextrin.

224308:BSU 12750
gene id: 224308:BSU_12750
uniprot id: P54341
names: Similarity
messages: To B.subtilis YqcC.

224308:BSU 39760
gene id: 224308:BSU_39760
uniprot id: A0A6M4JQU3
uniprot id: P42412
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the aldehyde dehydrogenase family. IolA subfamily.
messages: Catalyzes the oxidation of malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively (PubMed:16332250, PubMed:18310071, PubMed:21515690). Is involved in a myo-inositol catabolic pathway (PubMed:18310071). Bicarbonate, and not CO2, is the end-product of the enzymatic reaction (PubMed:16332250).
messages: Catalyzes the oxidation of malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively.
messages: Homotetramer.
messages: No effect on vanillin degradation.

224308:BSU 24250
gene id: 224308:BSU_24250
uniprot id: A0A6M3ZDI2
uniprot id: P17893
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ArgR family.
messages: Cytoplasm
messages: Homohexamer.
messages: Regulates arginine biosynthesis genes.
messages: Represses the synthesis of biosynthetic enzymes and activates the arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF.

224308:BSU 27070
gene id: 224308:BSU_27070
uniprot id: A0A6M4JNK0
uniprot id: P26379
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By fructose and LevR.
messages: Cytoplasm
messages: LevD and LevE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator.
messages: The EIIA type-4 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB type-4 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LevDE PTS system is involved in fructose transport.

224308:BSU 04150
gene id: 224308:BSU_04150
uniprot id: P42972
names: Similarity
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

224308:BSU 22040
gene id: 224308:BSU_22040
uniprot id: A0A6M3ZDI3
uniprot id: P54158
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 14640
gene id: 224308:BSU_14640
uniprot id: A0A6M3ZAC6
uniprot id: Q45497
names: Similarity
messages: Belongs to the UPF0223 family.

224308:BSU 17580
gene id: 224308:BSU_17580
uniprot id: A0A6M3ZBF4
uniprot id: P94489
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 43 family.
messages: Cell membrane
messages: Homodimer.
messages: Up-regulated by xylose. Subject to carbon catabolite repression (CCR).

224308:BSU 07990
gene id: 224308:BSU_07990
uniprot id: O34969
names: Similarity
messages: Belongs to the HIBADH-related family.

224308:BSU 40060
gene id: 224308:BSU_40060
uniprot id: A0A6M3ZIZ8
uniprot id: P12011
names: Activity Regulation
names: Similarity
messages: Belongs to the FGGY kinase family.
messages: Catabolite repression by gluconate.

224308:BSU 03280
gene id: 224308:BSU_03280
uniprot id: A0A6M3Z792
uniprot id: P42437
names: Induction
names: Similarity
messages: Belongs to the precorrin methyltransferase family.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 22350
gene id: 224308:BSU_22350
uniprot id: P39787
names: Function
messages: Probable component of primosome involved in the initiation of DNA replication.

224308:BSU 37450
gene id: 224308:BSU_37450
uniprot id: P71003
names: Disruption Phenotype
names: Subunit
messages: Interacts with PcrA. The interaction is not essential for cell viability or repair of UV-induced lesions.
messages: No PcrA-related phenotype.

224308:BSU 37970
gene id: 224308:BSU_37970
uniprot id: A0A6M3ZHQ1
uniprot id: P39615
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.
messages: Cytoplasm
messages: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
messages: Interacts with bacillus phage phi29 protein p56; this interaction inhibits the uracil-DNA glycosylase.

224308:BSU 02520
gene id: 224308:BSU_02520
uniprot id: P42242
names: Similarity
messages: Belongs to the aminoglycoside phosphotransferase family.

224308:BSU 09770
gene id: 224308:BSU_09770
uniprot id: O07545
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YheC/YheD family.
messages: Expressed during sporulation (at protein level).
messages: Forespore outer membrane
messages: Involved in sporulation.
messages: Spore coat

224308:BSU 29410
gene id: 224308:BSU_29410
uniprot id: A0A6M3ZFF9
uniprot id: Q795U4
names: Similarity
messages: Belongs to the UPF0173 family.

224308:BSU 10940
gene id: 224308:BSU_10940
uniprot id: A0A6M3Z9Q3
uniprot id: O06738
names: Similarity
messages: Belongs to the ComB family.

224308:BSU 39280
gene id: 224308:BSU_39280
uniprot id: P40738
names: Disruption Phenotype
messages: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon has no visible growth phenotype, however it is out-competed by wild-type cells.

224308:BSU 00880
gene id: 224308:BSU_00880
uniprot id: A0A6M3Z6M0
uniprot id: P37573
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DisA family.
messages: Cytoplasm
messages: Decreases c-di-AMP levels in mid-exponential phase from about 3.8 uM to about 2.8 uM in strain BG214 (PubMed:25616256). No change in sensitivity to methyl methanesulfonate (MMS), decreased survival after UV irradiation in transition and stationary phase, strong decrease in competence (PubMed:9141693). Cells lacking this gene show a reduced c-di-AMP level compared to wild-type and cannot properly trigger the DNA damage response (PubMed:21566650). No effect on antibiotic sensitivity to the beta-lactam antibiotic cefuroxime (CEF), upon overexpression of the c-di-AMP phosphodiesterase GdpP increased sensitivity to CEF (PubMed:22211522). Double disA-cdaA mutants cannot be made, suggesting they are lethal, while double disA-cdaS and cdaA-cdaS mutants are viable (PubMed:22211522, PubMed:23192352). Depletion of cdaA in double disA-cdaA deletion cells leads to cell lysis (PubMed:22211522). Exponentially growing cells are 100-fold more sensitive to MMS, no change in response to H(2)O(2) or nalidixic acid; a double disA-radA deletion suppresses H(2)O(2) sensitivity of the radA mutant, but has no effect on MMS sensitivity, suggesting radA and disA might work in the same DNA repair pathway (PubMed:25616256).
messages: Diadenylate cyclase activity is inhibited by the interaction with RadA (By similarity). Diadenylate cyclase activity is not affected by ssDNA or dsDNA, but three- and four-way junctions strongly inhibit the activity of DisA, suggesting the enzyme is regulated by branched nucleic acids.
messages: Expression increases during late exponential phase and at the onset of sporulation (PubMed:16713562). Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).
messages: Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome, operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis.
messages: Homooctamer.
messages: Homooligomer (PubMed:18439896). Interacts with RadA (PubMed:23760274).
messages: One of 3 paralogous diadenylate cyclases (DAC) in this bacteria (PubMed:23192352). Has diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (PubMed:18439896, PubMed:25616256). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis. Does not convert GTP to c-di-GMP (PubMed:18439896).
messages: Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged (PubMed:9141693, PubMed:16713562). Forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. Its ability to scan through the chromosome rapidly is due to its non-specific DNA-binding. When a lesion is present, DisA pauses at the lesion site (PubMed:21566650). This triggers a cellular response that culminates in a temporary block in sporulation initiation. It is required, at least partially, to inhibit the activity of the transcription factor spo0A, which controls, among others, early sporulation genes. In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis (PubMed:22211522); it is toxic when present in excess (PubMed:26240071).
messages: Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. DisA forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation.

224308:BSU 19200
gene id: 224308:BSU_19200
uniprot id: A0A6M4JJX0
uniprot id: O34723
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase.
messages: Phosphorylated by DesK.

224308:BSU 12410
gene id: 224308:BSU_12410
uniprot id: A0A6M3Z9P0
uniprot id: O34334
names: Similarity
names: Subunit
messages: Belongs to the DinB family.
messages: Homodimer.

224308:BSU 30860
gene id: 224308:BSU_30860
uniprot id: A0A6M4JM99
uniprot id: O34862
names: Function
names: Similarity
messages: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
messages: Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid.

224308:BSU 40900
gene id: 224308:BSU_40900
uniprot id: A0A6M3ZIA8
uniprot id: P37455
names: Caution
names: Function
names: Induction
names: PTM
names: Subunit
messages: Homotetramer.
messages: Homotetramer. Interacts with several proteins involved in DNA metabolism such as PriA, RecQ, RecG, RecS, DnaE, RarA, RecJ, RecO, SbcC, YrrC, XseA and Ung.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Phosphorylated by YwqD, which increases ssDNA affinity; dephosphorylated by YwqE.
messages: Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (PubMed:21170359). Has a 20-fold higher affinity for ssDNA than SsbB; SsbA and DprA activate the homologous DNA strand exchange function of RecA-ATP (PubMed:25138221).
messages: Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.
messages: Strongly expressed during exponential growth, decreases 2-4-fold in stationary phase, part of the rpsF-ssbA-rpsR and the ychF-rpsF-ssbA-rpsR operons (PubMed:14762004). The operon is induced by DNA damage by mitomycin C (PubMed:14762004).

224308:BSU 01660
gene id: 224308:BSU_01660
uniprot id: A0A6M4JEW2
uniprot id: P40406
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 3 family.
messages: Cell membrane
messages: Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides. Cleaves muropeptides, but not peptidoglycan.
messages: Up-regulated during the late phase of exponential growth and during stationary phase.
messages: cell wall

224308:BSU 23430
gene id: 224308:BSU_23430
uniprot id: P40867
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 38590
gene id: 224308:BSU_38590
uniprot id: P46318
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lichenan transport.

224308:BSU 37760
gene id: 224308:BSU_37760
uniprot id: A0A6M4JQC9
uniprot id: P39636
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: By arginine.
messages: Cell membrane
messages: Membrane
messages: Putative transport protein involved in arginine degradative pathway. Probably transports arginine or ornithine.

224308:BSU 07770
gene id: 224308:BSU_07770
uniprot id: O35036
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 22340
gene id: 224308:BSU_22340
uniprot id: A0A6M3ZDE9
uniprot id: P39788
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the Nth/MutY family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Cells lacking this gene have a 5-fold increased spontaneous mutation frequency. A triple MutS/MutL/nth disruption has a 280-fold increased spontaneous mutation frequency.
messages: DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

224308:BSU 35630
gene id: 224308:BSU_35630
uniprot id: Q02113
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: In stationary phase; under control of SigD (PubMed:11987133). Repressed by LytR.
messages: Possibly involved in cell wall metabolism during spore formation. Enhances the amidase activity approximately threefold.
messages: cell wall

224308:BSU 33550
gene id: 224308:BSU_33550
uniprot id: O32225
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YccS/YhfK family.
messages: Cell membrane

224308:BSU 07800
gene id: 224308:BSU_07800
uniprot id: A0A6M3Z8H7
uniprot id: P39794
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: B.subtilis does not possess a trehalose-specific phosphotransferase enzyme IIA component, however it seems that it use the glucose-specific phosphotransferase enzyme IIA component to delivers trehalose-6-phosphate into the cell.
messages: Cell membrane
messages: Induced by trehalose-6-phosphate. Repressed by TreR.
messages: Membrane
messages: The EIIC domain type-1 forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA-Glc (EIII-Glc) on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in trehalose transport.

224308:BSU 38430
gene id: 224308:BSU_38430
uniprot id: P25148
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 8 family.
messages: By different stresses such as heat shock and salt stress and by starvation.

224308:BSU 24830
gene id: 224308:BSU_24830
uniprot id: P54497
names: Similarity
messages: Belongs to the peptidase M14 family.

224308:BSU 38510
gene id: 224308:BSU_38510
uniprot id: A0A6M3ZHS7
uniprot id: P39580
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the membrane-bound acyltransferase family.
messages: Cell membrane
messages: Consists of a ring of transmembrane domains, which shield a highly conserved extracellular structural funnel extending into the middle of the lipid bilayer. The conserved catalytic His residue is located at the bottom of this funnel and is connected to the intracellular DltC through a narrow tunnel.
messages: Membrane
messages: O-acyltransferase that catalyzes D-alanylation of both teichoic acid and lipoteichoic acid (LTA) (PubMed:30283133). D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall (PubMed:7797557). Catalyzes D-alanylation from DltC carrier protein (PubMed:30283133).
messages: O-acyltransferase that catalyzes D-alanylation of both teichoic acid and lipoteichoic acid (LTA). D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. Catalyzes D-alanylation from DltC carrier protein.

224308:BSU 33740
gene id: 224308:BSU_33740
uniprot id: A0A6M3ZGN8
uniprot id: C0SPB8
names: Similarity
messages: Belongs to the GbsR family.

224308:BSU 24870
gene id: 224308:BSU_24870
uniprot id: A0A6M3ZE41
uniprot id: P54493
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S54 family.
messages: Cell membrane
messages: Inhibited by dichloroisocoumarin (DCI) and N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), but not by other serine protease inhibitors such as sulfonyl fluoride PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF).
messages: Membrane
messages: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Important for normal cell division and sporulation. May act as a glucose exporter.

224308:BSU 31090
gene id: 224308:BSU_31090
uniprot id: O32080
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the KtrA potassium transport family.
messages: Catalytic subunit of the KtrAB potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.
messages: Cell membrane
messages: Homodimer, tetramer (dimer of homodimer) and octamer (tetramer of homodimer). Part of the KtrAB complex formed by an octameric catalytic ring of KtrA and a membrane associated dimer of KtrB forming a potassium channel.
messages: Impaired potassium uptake.

224308:BSU 03560
gene id: 224308:BSU_03560
uniprot id: A0A6M3Z7U7
uniprot id: Q08792
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 03150
gene id: 224308:BSU_03150
uniprot id: A0A6M3Z7Q1
uniprot id: P37944
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the shikimate kinase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 14450
gene id: 224308:BSU_14450
uniprot id: A0A6M4JHU0
uniprot id: P39762
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the peptidase M29 family.
messages: Binds 2 divalent metal cations per subunit. Can use cobalt, zinc, and possibly also magnesium ions.
messages: Metal-dependent exopeptidase.

224308:BSU 10640
gene id: 224308:BSU_10640
uniprot id: A0A6M4JET8
uniprot id: P23479
names: Function
names: Similarity
names: Subunit
messages: Belongs to the SbcD family.
messages: Heterodimer of SbcC and SbcD.
messages: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity (By similarity).
messages: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.

224308:BSU 32610
gene id: 224308:BSU_32610
uniprot id: A0A6M3ZGM8
uniprot id: O32157
names: Function
names: Subunit
messages: Catalyzes the conversion of a range of fructosamine 6-phosphates to glucose 6-phosphate and a free amino acid.
messages: Homooctamer.

224308:BSU 03640
gene id: 224308:BSU_03640
uniprot id: A0A6M4JF35
uniprot id: P94405
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: A triple bsdB-bsdC-bsdD deletion mutant no longer converts vanillin to guaiacol, the conversion stops at vanillic acid (PubMed:26658822).
messages: Belongs to the UbiD family. YclC subfamily.
messages: Binds 1 prenylated FMN (prenyl-FMN) per subunit.
messages: Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives under both aerobic and anaerobic conditions (PubMed:15979273, PubMed:18388975). Phenolic acid decarboxylase that catalyzes the reversible decarboxylation of 4-hydroxybenzoate and vanillate (PubMed:15979273, PubMed:18388975). Could also catalyze the decarboxylation of salicylate (Probable). Is not active on di- and tri-hydroxybenzoate derivatives (PubMed:18388975).
messages: Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives.
messages: It is not known, if phenolic acid decarboxylase forms a complex composed of BsdB, BsdC and BsdD. The term subunit is often used in reference to the operon, however there is no experimental evidence to prove the existence of the complex.
messages: Up-regulated by salicylate via the transcriptional regulator BsdA.

224308:BSU 03300
gene id: 224308:BSU_03300
uniprot id: P42435
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Binds 1 siroheme per subunit.
messages: Homodimer.
messages: Positively regulated by TnrA under nitrogen-limited conditions. Induced by ArfM.
messages: Required for nitrite assimilation.

224308:BSU 14071
gene id: 224308:BSU_14071
uniprot id: O34320
names: Induction
names: Similarity
messages: Belongs to the FadG family.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 21770
gene id: 224308:BSU_21770
uniprot id: A0A6M3ZDA0
uniprot id: P37946
names: Function
names: Similarity
names: Subunit
messages: Belongs to the serine/threonine dehydratase family.
messages: Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity).
messages: Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.
messages: Homotetramer.

224308:BSU 31140
gene id: 224308:BSU_31140
uniprot id: A0A6M4JMJ0
uniprot id: O32085
names: Cofactor
names: Developmental Stage
names: Miscellaneous
names: Similarity
messages: Although there are Cys and Trp residues in equivalent positions with the residues forming a thioether cross-link in the eukaryotic homologous sequences, no thioether cross-link is formed in this protein.
messages: Belongs to the cysteine dioxygenase family.
messages: Binds 1 Fe cation per subunit.
messages: Up-regulated upon sporulation.

224308:BSU 25420
gene id: 224308:BSU_25420
uniprot id: A0A6M3ZE21
uniprot id: P54463
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: To E.coli YjbB.

224308:BSU 41050
gene id: 224308:BSU_41050
uniprot id: A0A6M4JR78
uniprot id: P25814
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RnpA family.
messages: Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.
messages: Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit. Might be a component of the prossible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno, and possibly also rnpA. Interacts with RNA helicase CshA.
messages: RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.

224308:BSU 27740
gene id: 224308:BSU_27740
uniprot id: A0A6M3ZEZ7
uniprot id: O05392
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RuvA family.
messages: Cytoplasm
messages: Has three domains with a flexible linker between the domains II and III and assumes an 'L' shape. Domain III is highly mobile and contacts RuvB.
messages: Homotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.

224308:BSU 38320
gene id: 224308:BSU_38320
uniprot id: A0A6M3ZHQ9
uniprot id: P39591
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CidA/LrgA family. CidA subfamily.
messages: Cell membrane
messages: Increases the activity of extracellular murein hydrolases possibly by mediating their export via hole formation. Inhibited by the antiholin-like proteins LrgAB. In an unstressed cell, the LrgAB products probably inhibit the function of the CidA protein. When a cell is stressed by the addition of antibiotics or by other factors in the environment, CidA possibly oligomerizes within the bacterial cell membrane, creating lesions that disrupt the proton motive force, which in turn results in loss of cell viability. These lesions are also hypothesized to regulate the subsequent cell lysis by either allowing the murein hydrolases access to the cell wall substrate and/or regulating their activity by a possible change in the cell wall pH that results from loss of membrane potential.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane

224308:BSU 38850
gene id: 224308:BSU_38850
uniprot id: P94356
names: Induction
names: Subcellular Location Annotation
messages: Secreted
messages: Transcriptionally regulated by SigD. Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 23270
gene id: 224308:BSU_23270
uniprot id: P16440
names: Activity Regulation
names: Function
names: Subunit
messages: Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.
messages: Homotrimer. Can interact with 6,7-dimethyl-8-ribityllumazine synthase, forming a lumazine synthase/riboflavin synthase complex, also designated as 'heavy riboflavin synthase complex', which consists of a trimer of riboflavin synthase enclosed within an icosahedral structure composed of 60 subunits of 6,7-dimethyl-8-ribityllumazine synthase.
messages: Is activated by sulfite ions.

224308:BSU 19790
gene id: 224308:BSU_19790
uniprot id: P42090
names: Caution
names: Function
messages: It is uncertain whether Met-1, Met-44 or Met-88 is the initiator.
messages: May be involved in maturation of the outermost layer of the spore.

224308:BSU 26860
gene id: 224308:BSU_26860
uniprot id: A0A6M4JMQ1
uniprot id: O05407
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CitM (TC 2.A.11) transporter family.
messages: Cell membrane
messages: Membrane
messages: Transports the free citrate anion.

224308:BSU 35420
gene id: 224308:BSU_35420
uniprot id: P39808
names: Function
messages: May be involved in the assembly, structure, or function of the flagellum. May polymerize to form a filamentous structure that is part of the flagellum.

224308:BSU 15390
gene id: 224308:BSU_15390
uniprot id: A0A6M4JGI1
uniprot id: O31728
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SepF family.
messages: Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA.
messages: Cytoplasm
messages: Homodimer. Interacts with FtsZ.

224308:BSU 37370
gene id: 224308:BSU_37370
uniprot id: P71011
names: Cofactor
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine, the other is coordinated via 3 cysteines and maybe direct contact with the SboA precursor.
messages: Catalyzes the formation of 3 thioether bonds during production of the sactipeptide subtilosin from SboA. In vitro the thioether bonds cannot be made in the absence of the SboA propeptide, suggesting this is the first reaction in subtilosin maturation (PubMed:22366720). In vitro, in the absence of a second substrate, cleaves S-adenosyl-L-methionine into Met and 5'-dA (PubMed:22366720).
messages: Cytoplasm
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 05620
gene id: 224308:BSU_05620
uniprot id: A0A6M3Z7V3
uniprot id: P96704
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane
messages: Probable amino-acid or metabolite transport protein.

224308:BSU 23100
gene id: 224308:BSU_23100
uniprot id: A3F488
uniprot id: P35165
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: Cell membrane
messages: Induced 3-fold by the beta-lactam antibiotic cefuroxime.
messages: Interacts transiently with the RNAP core.
messages: No effect on antibiotic resistance; double sigM-sigX mutants have 130-fold increases sensitivity to the beta-lactam antibiotic cefuroxime (PubMed:22211522). Association with RNAP core increases during many stresses (at protein level) (PubMed:21710567).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. May be involved in the regulation of iron metabolism. Associates with RNAP core during early growth phases, association decreases as cells age (PubMed:21710567).

224308:BSU 30680
gene id: 224308:BSU_30680
uniprot id: A0A6M4JL66
uniprot id: O34601
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0161 family.
messages: Cell membrane
messages: Could be involved in insertion of integral membrane proteins into the membrane.

224308:BSU 18730
gene id: 224308:BSU_18730
uniprot id: O31833
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 04400
gene id: 224308:BSU_04400
uniprot id: P26907
names: Domain
names: Function
names: Induction
names: Miscellaneous
messages: Consists mostly of well conserved tandem repeats.
messages: Glucose deprivation induces sporulation and several other adaptive responses.
messages: Glucose or phosphate starvation, and addition of decoyinine. Also by heat shock, salt stress and oxidative stress.
messages: Involved in an adaptive response to nutrient deprivation other than sporulation.

224308:BSU 20929
gene id: 224308:BSU_20929
uniprot id: C0H438
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 22490
gene id: 224308:BSU_22490
uniprot id: A0A6M4JN90
uniprot id: P42976
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DapB family.
messages: Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.
messages: Cytoplasm
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

224308:BSU 31580
gene id: 224308:BSU_31580
uniprot id: A0A6M3ZFT0
uniprot id: O05256
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Activated by the two-component regulatory system MalK/MalR in response to malate. The regulator MalR binds to the promoter region of maeN.
messages: Acts as a Na(+)-malate symporter, as it catalyzes malate-dependent uptake of Na(+) and Na(+)-dependent uptake of malate.
messages: Belongs to the sodium:citrate (SCF) symporter family.
messages: Cell membrane
messages: Membrane

224308:BSU 16910
gene id: 224308:BSU_16910
uniprot id: O31771
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 35410
gene id: 224308:BSU_35410
uniprot id: A0A6M4JPR1
uniprot id: P39810
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum
messages: Belongs to the flagella basal body rod proteins family.
messages: Secreted

224308:BSU 37200
gene id: 224308:BSU_37200
uniprot id: A0A6M3ZLE6
uniprot id: P45864
names: Function
names: Similarity
messages: Belongs to the uve1/UvsE family.
messages: Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion (By similarity).
messages: Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion.
messages: Component in a DNA repair pathway. Removal of UV-light damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion.

224308:BSU 15800
gene id: 224308:BSU_15800
uniprot id: O34664
names: Function
names: Similarity
messages: Belongs to the thiamine pyrophosphokinase family.
messages: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate.

224308:BSU 30620
gene id: 224308:BSU_30620
uniprot id: A0A6M3ZFQ9
uniprot id: O34649
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 30450
gene id: 224308:BSU_30450
uniprot id: O34641
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Expressed early in the stationary phase.
messages: Negatively regulated by YtrA.
messages: Part of the ABC transporter complex YtrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.
messages: The complex is composed of 2 ATP-binding proteins (YtrB and YtrE), 2 transmembrane proteins (YtrC and YtrD) and a solute-binding protein (YtrF).

224308:BSU 06500
gene id: 224308:BSU_06500
uniprot id: A0A6M3Z814
uniprot id: P12043
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AIR synthase family.
messages: Cytoplasm

224308:BSU 04300
gene id: 224308:BSU_04300
uniprot id: P96587
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 2 family.
messages: Membrane

224308:BSU 26670
gene id: 224308:BSU_26670
uniprot id: A0A6M3ZEP9
uniprot id: P94501
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.
messages: No effect observed.
messages: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression.

224308:BSU 08070
gene id: 224308:BSU_08070
uniprot id: O34591
names: Function
names: Subunit
messages: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde.
messages: Tetramer of 2 alpha and 2 beta subunits.

224308:BSU 06750
gene id: 224308:BSU_06750
uniprot id: O35034
names: Similarity
messages: Belongs to the site-specific recombinase resolvase family.

224308:BSU 33780
gene id: 224308:BSU_33780
uniprot id: A0A6M3ZGC3
uniprot id: O32241
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By Spo0A during nutrient starvation, through its direct negative control of AbrB, repressed by AbrB during regular growth when nutrients are plentiful, and also by SdpR (PubMed:16469701). Induced by expression of SDP (active peptide of sdpC) (PubMed:16469701, PubMed:23687264).
messages: Immunity protein that provides protection for the cell against the toxic effects of SDP, its own SdpC-derived killing factor, and that functions as a receptor/signal transduction protein as well. Once SDP accumulates in the extracellular milieu, SdpI binds to SDP, causing sequestration of SdpR at the bacterial membrane.
messages: Membrane
messages: Results in increased SigW expression which is dependent on SdpC. Results in weaker growth (70% of wild-type cell density) on LB medium, with more pronounced effects on sporulation medium (15% of wild-type). Concomitant deletion of SigW results in greatly diminished growth (5% of wild-type on LB and 3% on sporulation medium). Over time, single SdpI mutant stops growing on sporulation medium, while the SdpI SigW double mutant eventually lyses. Deletion of RsiW restores growth of the SdpI single mutant to near wild-type levels.

224308:BSU 34530
gene id: 224308:BSU_34530
uniprot id: A0A6M4JPF3
uniprot id: O06996
names: Caution
names: Developmental Stage
names: Subcellular Location Annotation
messages: Expressed during sporulation, in the late phase of coat protein synthesis.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Spore coat

224308:BSU 26780
gene id: 224308:BSU_26780
uniprot id: A0A6M3ZE85
uniprot id: O07079
names: Similarity
messages: Belongs to the DinB family.

224308:BSU 23390
gene id: 224308:BSU_23390
uniprot id: A0A6M4JKD7
uniprot id: P31845
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Membrane

224308:BSU 00370
gene id: 224308:BSU_00370
uniprot id: P08874
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription.
messages: Interacts with BrxC.
messages: It is uncertain whether Met-1 or Met-3 is the initiator.
messages: To B.subtilis Abh and SpoVT.

224308:BSU 18620
gene id: 224308:BSU_18620
uniprot id: C0SPC0
names: Function
names: Similarity
messages: Belongs to the FADH(2)-utilizing monooxygenase family.
messages: Catalyzes the hydroxylation of 4-hydroxyphenylacetic acid (4HPA), leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA).

224308:BSU 08300
gene id: 224308:BSU_08300
uniprot id: A0A6M4JE27
uniprot id: P94439
names: Disruption Phenotype
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Deletion mutant does not show any observable phenotype.
messages: Phosphorylated by LnrJ.
messages: Required for resistance to linearmycins, a family of antibiotic-specialized metabolites produced by some streptomycetes (PubMed:26647299, PubMed:28461449). Member of the two-component regulatory system LnrJ/LnrK, which induces expression of the LnrLMN ABC transporter in response to linearmycins and other polyenes (PubMed:11717295, PubMed:26647299, PubMed:28461449). Probably binds to the promoter region of the lnrLMN operon and directly regulates its expression (Probable). May also promote biofilm formation (PubMed:28461449).

224308:BSU 06300
gene id: 224308:BSU_06300
uniprot id: P07788
names: Activity Regulation
names: Biotechnology
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the multicopper oxidase family.
messages: Binds 4 copper ions per subunit. The 4 copper centers adopt structures classified as type 1, type 2 and type 3.
messages: CotA is also a promising candidate for the clinical determination of direct bilirubin and total bilirubin, which are significant diagnostic marker of liver function and cancer.
messages: Disruption of the gene does not prevent spore formation, but mutant is blocked in the appearance of the brown pigment characteristic of colonies of wild-type sporulating cells.
messages: Expression is switched on about four to five hours after the onset of sporulation, a time that corresponds approximately to the stage of spore coat synthesis and deposition.
messages: Glu-498, located within the entrance channel of the trinuclear center, plays a key role in the protonation events that occur at the trinuclear center and in its stabilization, controlling therefore the binding of dioxygen and its further reduction (PubMed:20822511, PubMed:20200715). Asp-116, situated in the exit channel of the trinuclear center, and its hydrogen bond connectivity are highly relevant for the overall reactivity of the laccase but do not appear to play a critical structural role (PubMed:22481612).
messages: In vitro, also shows strong bilirubin oxidase (BOD) activity, and can catalyze the oxidation of free bilirubin (UB), direct bilirubin (conjugated with glucuronic acid, DB) and ditaurobilirubin.
messages: Inhibited by azide.
messages: Monomer.
messages: Multicopper oxidase that catalyzes the oxidation of a variety of substrates, including phenolic and non-phenolic compounds. Substrates include syringaldazine (SGZ), 2,6-dimethoxyphenol (2,6-DMP) and the non-phenolic compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (PubMed:11514528, PubMed:11884407, PubMed:18307408, PubMed:20200715, PubMed:22481612, PubMed:27050268). Has no tyrosinase activity (PubMed:11514528). Is implicated in the biosynthesis of a brownish pigment that characterizes sporulating colonies of B.subtilis, and which appears to be a melanin-like product and to confer protection against UV light (PubMed:2821284, PubMed:11514528, PubMed:11884407).
messages: Spore coat
messages: When expressed on the surface of E.coli cells, CotA was shown to possess improved enzymatic properties, including higher thermal stability and stronger inhibitor tolerance. It has high enzymatic activity against three industrial dye types: an anthraquinone dye, Acid Blue 62, a triphenylmethane dye, Malachite Green, and an azo dye, Methyl Orange. It may help decrease the costs and simplify wastewater treatment, and therefore, surface-displayed CotA shows a great potential for industrial-scale dye decolorization.

224308:BSU 21550
gene id: 224308:BSU_21550
uniprot id: O31995
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 31321
gene id: 224308:BSU_31321
uniprot id: Q5BU39
names: Biotechnology
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: As a fusion partner, can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
messages: Cell membrane
messages: Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.
messages: Monomer.

224308:BSU 18090
gene id: 224308:BSU_18090
uniprot id: A0A6M4JJ54
uniprot id: Q59192
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the type II topoisomerase family. ParE type 2 subfamily.
messages: Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).
messages: Heterotetramer composed of ParC and ParE.
messages: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.

224308:BSU 34290
gene id: 224308:BSU_34290
uniprot id: P71058
names: Function
names: Induction
names: Similarity
messages: Belongs to the polysaccharide pyruvyl transferase family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Repressed by SinR.

224308:BSU 33340
gene id: 224308:BSU_33340
uniprot id: Q7WY58
names: Developmental Stage
names: Induction
names: Subcellular Location Annotation
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expression is sigma G-dependent, and to a very small extent sigma F-dependent.
messages: Spore core

224308:BSU 28200
gene id: 224308:BSU_28200
uniprot id: A0A6M3ZF30
uniprot id: P37945
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity). Has been implicated in preventing sigma(G) activity under non-sporulation conditions.
messages: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.
messages: Belongs to the peptidase S16 family.
messages: By heat shock.
messages: Cytoplasm
messages: Homohexamer. Organized in a ring with a central cavity (By similarity). Exists as a mixture of small oligomeric species in solution.
messages: Homohexamer. Organized in a ring with a central cavity.

224308:BSU 27520
gene id: 224308:BSU_27520
uniprot id: O34527
names: Disruption Phenotype
names: Function
names: Subunit
messages: Grows slowly with cystine as sole sulfur source.
messages: Homodimer. Forms homotetramers at higher concentrations of protein. Forms CymR(2):CysK(2) or CymR(4):CysK(4) complexes in the absence of O-acetylserine.
messages: Master repressor of cysteine metabolism in B.subtilis. Controls the expression of genes involved either in cysteine synthesis from sulfide (cysK), sulfonates (ssu), or methionine (mccAB) or in cystine uptake (tcyP). Activity of CymR is positively regulated by CysK in response to cysteine availability. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression.

224308:BSU 07700
gene id: 224308:BSU_07700
uniprot id: A0A6M4JFX9
uniprot id: O34521
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Expression is repressed by the HTH-type transcriptional regulator NagR.
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft
messages: The EIIC domain type-1 forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylglucosamine transport (By similarity).

224308:BSU 28530
gene id: 224308:BSU_28530
uniprot id: A0A6M4JKI4
uniprot id: P94550
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the ETF beta-subunit/FixA family.
messages: Binds 1 AMP per subunit.
messages: Binds 1 FAD per dimer.
messages: Heterodimer of an alpha and a beta subunit.
messages: The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity).

224308:BSU 13770
gene id: 224308:BSU_13770
uniprot id: O31680
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 36980
gene id: 224308:BSU_36980
uniprot id: P39150
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0715 family.
messages: Cell membrane

224308:BSU 09965
gene id: 224308:BSU_09965
uniprot id: O07518
names: Caution
names: Subcellular Location Annotation
messages: Cell membrane
messages: Was initially thought to be two separate ORFs named yhaJ and yhaK.

224308:BSU 03530
gene id: 224308:BSU_03530
uniprot id: A0A6M4JF27
uniprot id: Q08791
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 31460
gene id: 224308:BSU_31460
uniprot id: Q08429
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: May play a role in the activation or the expression of KinB.

224308:BSU 18290
gene id: 224308:BSU_18290
uniprot id: O34506
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0713 family.
messages: Cell membrane

224308:BSU 15540
gene id: 224308:BSU_15540
uniprot id: A0A6M3ZAL4
uniprot id: P25996
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.
messages: Binds 1 FMN per subunit.
messages: Catalyzes the conversion of dihydroorotate to orotate with NAD(+) as electron acceptor.
messages: Cytoplasm
messages: Heterotetramer of 2 PyrK and 2 PyrD type B subunits.

224308:BSU 30290
gene id: 224308:BSU_30290
uniprot id: A0A6M4JNY6
uniprot id: O34518
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Deletion of the gene abolishes induction in the presence of melibiose and raffinose.
messages: Membrane
messages: Part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import. Probably responsible for the translocation of the substrate across the membrane.
messages: Repressed by the transcriptional regulator MelR. Induced by melibiose and raffinose.
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MelC and MelD) and a solute-binding protein (MelE).

224308:BSU 31180
gene id: 224308:BSU_31180
uniprot id: A0A6M3ZFZ2
uniprot id: O05264
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the rhamnose isomerase family.
messages: Binds 1 Mn(2+) ion per subunit.
messages: Catalyzes the interconversion of L-rhamnose and L-rhamnulose.
messages: Cytoplasm

224308:BSU 21410
gene id: 224308:BSU_21410
uniprot id: O31982
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
messages: Secreted

224308:BSU 39000
gene id: 224308:BSU_39000
uniprot id: P42314
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CitM (TC 2.A.11) transporter family.
messages: Cell membrane

224308:BSU 40180
gene id: 224308:BSU_40180
uniprot id: Q45596
names: Function
names: Induction
messages: Repressed by AbrB. Induced in stationary phase in LB (rich) medium; the transcript is present in log phase cells grown in modified competence (MC) and MM media.
messages: Suggested to be the precursor for an exported, modified peptide that has antimicrobial and/or signaling properties. Synthesis requires YydG and YydH; the peptide is proposed to be exported by the YydIJ transporter. In the absence of the transporter, the modified peptide activates the LiaRS two-component regulatory system, possibly by eliciting cell envelope stress. This activation can occur in trans in cocultured cells lacking either the transporter or the whole operon.

224308:BSU 31350
gene id: 224308:BSU_31350
uniprot id: A0A6M4JN96
uniprot id: P80860
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GPI family.
messages: Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 35940
gene id: 224308:BSU_35940
uniprot id: P36947
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Ribose importer (TC 3.A.1.2.1) family.
messages: Cell membrane
messages: Part of the ABC transporter complex RbsABC involved in ribose import. Responsible for energy coupling to the transport system.
messages: The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB).

224308:BSU 03240
gene id: 224308:BSU_03240
uniprot id: P94394
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0703 family.
messages: Cell membrane

224308:BSU 12950
gene id: 224308:BSU_12950
uniprot id: A0A6M3Z9W0
uniprot id: P26905
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Probably part of the ABC transporter DppBCDE involved in dipeptide transport (Probable). Responsible for energy coupling to the transport system (Probable).

224308:BSU 06990
gene id: 224308:BSU_06990
uniprot id: A0A6M4JFV5
uniprot id: O31520
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 02130
gene id: 224308:BSU_02130
uniprot id: P37965
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycerophosphoryl diester phosphodiesterase family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Deletion mutant retains WTA and ceases growth upon phosphate limitation (PubMed:27780866). Mutants show altered cell morphologies and effects on autolytic activity and antibiotic susceptibilities that, unexpectedly, also occur in phosphate-replete conditions (PubMed:27780866).
messages: Glycerophosphodiester phosphodiesterase hydrolyzes glycerophosphodiesters into glycerol-3-phosphate (G3P) and the corresponding alcohol (PubMed:27780866). Involved in wall teichoic acid (WTA) metabolism during phosphate starvation (PubMed:27780866). Catalyzes the degradation of WTA, enabling the utilization of WTA as a phosphate reserve under limiting conditions (PubMed:27780866). Is highly selective for the poly(gylcerol phosphate) WTA backbone and catalyzes exolytic cleavage of individual monomer units (PubMed:27780866). In vitro is active toward the WTA oligomer mimics glycerophosphoglycerol (GPG) and bis-glycerophosphoglycerol (bGPG) (PubMed:27780866).
messages: Secreted
messages: Transcriptionally regulated by PhoP.

224308:BSU 39620
gene id: 224308:BSU_39620
uniprot id: P54940
names: Induction
messages: Transcriptionally regulated by YxdJ. Induced by the antibacterial protein LL-37, probably via YxdJ.

224308:BSU 24170
gene id: 224308:BSU_24170
uniprot id: A0A6M3ZDH6
uniprot id: P45855
names: Developmental Stage
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thiolase-like superfamily. Thiolase family.
messages: Cytoplasm
messages: Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
messages: Subject to catabolite repression.

224308:BSU 39820
gene id: 224308:BSU_39820
uniprot id: A0A6M3ZKH0
uniprot id: P46208
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the heat shock protein 90 family.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Molecular chaperone. Has ATPase activity.

224308:BSU 01010
gene id: 224308:BSU_01010
uniprot id: A0A6M3Z6I6
uniprot id: Q06795
names: Function
names: Similarity
messages: Belongs to the NusG family.
messages: Participates in transcription elongation, termination and antitermination.
messages: Participates in transcription elongation, termination and antitermination. Stimulates RNA polymerase pausing at U107 and U144 in the trp leader. NusG-stimulated pausing is sequence specific. Does not affect trp leader termination.

224308:BSU 17910
gene id: 224308:BSU_17910
uniprot id: A0A6M4JH54
uniprot id: P45708
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0154 family.
messages: Cell membrane
messages: Membrane

224308:BSU 13560
gene id: 224308:BSU_13560
uniprot id: A0A6M4JHJ9
uniprot id: O31663
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the methylthioribose kinase family.
messages: By starvation.
messages: Catalyzes the phosphorylation of methylthioribose into methylthioribose-1-phosphate.
messages: Homodimer.

224308:BSU 08780
gene id: 224308:BSU_08780
uniprot id: A0A6M3ZIF6
uniprot id: P71089
names: Similarity
messages: Belongs to the peptidase S51 family.

224308:BSU 13890
gene id: 224308:BSU_13890
uniprot id: A0A6M4JJE0
uniprot id: P20166
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

224308:BSU 24060
gene id: 224308:BSU_24060
uniprot id: A0A6M4JL69
uniprot id: P54533
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
messages: Binds 1 FAD per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: The active site is a redox-active disulfide bond.
messages: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 21350
gene id: 224308:BSU_21350
uniprot id: A0A6M3ZIA3
uniprot id: O31976
names: Similarity
messages: Belongs to the transglycosylase Slt family.

224308:BSU 16580
gene id: 224308:BSU_16580
uniprot id: A0A6M3ZAS8
uniprot id: P13267
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DNA polymerase type-C family. PolC subfamily.
messages: Cytoplasm
messages: Mutant azp12 has a form of DNA polymerase III resistant to hydroxyphenylazopyrimidines.
messages: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

224308:BSU 21580
gene id: 224308:BSU_21580
uniprot id: A0A6M3ZD79
uniprot id: O31998
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Deletion of the yokI-yokJ operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; not dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Probably interacts with cognate immunity protein YokJ but not with non-cognate immunity proteins. The interaction inhibits the toxic activity of YokJ (Probable).
messages: Secreted
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YokJ does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation (PubMed:34280190). The C-terminus (residues 449-571) inhibits growth upon expression in E.coli which is neutralized by cognate immunity protein YokJ, but not by immunity proteins specific to other toxins with the LXG domain (PubMed:22200572).
messages: Was originally suggested to be an RNase.

224308:BSU 27940
gene id: 224308:BSU_27940
uniprot id: A0A6M3ZF18
uniprot id: P05657
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL27 family.
messages: No effect on sporulation at 37 degrees Celsius, however sporulation decreases at 47 degrees Celsius.
messages: Part of the 50S ribosomal subunit.
messages: Plays a role in sporulation at high temperatures.

224308:BSU 38750
gene id: 224308:BSU_38750
uniprot id: A0A6M4JQL6
uniprot id: P94365
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome ubiquinol oxidase subunit 2 family.
messages: Binds 1 iron-chlorin (heme d or cytochrome d) per heterodimer, in conjunction with CydA.
messages: Binds 1 protoheme IX center (heme b595) per heterodimer, in conjunction with CydA.
messages: Cell membrane
messages: Heterodimer of subunits I and II.
messages: Membrane

224308:BSU 40022
gene id: 224308:BSU_40022
uniprot id: A0A6M3ZI23
uniprot id: P42102
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family.
messages: Cell membrane
messages: Membrane

224308:BSU 13250
gene id: 224308:BSU_13250
uniprot id: A0A6M4JL09
uniprot id: O34903
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Phosphorylated by YkoH.
messages: Probable member of the two-component regulatory system YkoH/YkoG.

224308:BSU 29590
gene id: 224308:BSU_29590
uniprot id: O34874
names: Function
names: Similarity
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
messages: Catalyzes the removal of elemental sulfur from cysteine to produce alanine.

224308:BSU 24410
gene id: 224308:BSU_24410
uniprot id: P49780
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 03420
gene id: 224308:BSU_03420
uniprot id: P12669
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Plays a role in the competence of cells to be transformed. It inhibits the activity of the DNA-entry nuclease.
messages: This protein is a subunit of a 75 kDa protein complex, which governs binding and entry of donor DNA. The complex is a tetramer of two subunits of the DNA-entry nuclease and two subunits of a competence-specific protein. Only the complex is able to bind ds- and ss-DNA.

224308:BSU 14250
gene id: 224308:BSU_14250
uniprot id: O31700
names: Induction
messages: Transcription starts around hour 2 of sporulation and requires sigma-E.

224308:BSU 16570
gene id: 224308:BSU_16570
uniprot id: A0A6M4JI95
uniprot id: O31755
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.
messages: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.
messages: Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 33350
gene id: 224308:BSU_33350
uniprot id: O32205
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To E.coli YdjM.

224308:BSU 35880
gene id: 224308:BSU_35880
uniprot id: A0A6M3ZGL0
uniprot id: P96738
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the CapA family.
messages: Cells produce PGA but at a greatly reduced level.
messages: Seems to be required for maximum PGA (gamma-polyglutamic acid) production.

224308:BSU 40740
gene id: 224308:BSU_40740
uniprot id: P37506
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the acetyltransferase family. GNAT subfamily.
messages: Deletion mutant is susceptible to streptothricin.
messages: Homodimer.
messages: Involved in resistance to streptothricin, a broad-spectrum antibiotic produced by streptomycetes. Detoxifies streptothricin via acetylation of the beta amino group of the first beta-lysyl moiety of streptothricin.

224308:BSU 35110
gene id: 224308:BSU_35110
uniprot id: A0A6M3ZHG4
uniprot id: O34719
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 24330
gene id: 224308:BSU_24330
uniprot id: A0A6M4JIZ8
uniprot id: P54519
names: Similarity
messages: Belongs to the asp23 family.

224308:BSU 22970
gene id: 224308:BSU_22970
uniprot id: A0A6M4JIW0
uniprot id: P50734
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MecA family.
messages: Enables the recognition and targeting of unfolded and aggregated proteins to the ClpC protease or to other proteins involved in proteolysis. Acts negatively in the development of competence by binding ComK and recruiting it to the ClpCP protease. When overexpressed, inhibits sporulation. Also involved in Spx degradation by ClpC.
messages: Enables the recognition and targeting of unfolded and aggregated proteins to the ClpC protease or to other proteins involved in proteolysis. Also involved in Spx degradation by ClpC (By similarity). Acts negatively in the development of competence by binding ComK and recruiting it to the ClpCP protease. When overexpressed, inhibits sporulation.
messages: Homodimer.
messages: The N-terminal domain has binding sites for ComK and probably for unfolded/aggregated proteins; the C-terminal domain interacts with ClpC.

224308:BSU 30260
gene id: 224308:BSU_30260
uniprot id: O34829
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Negatively autoregulated. Induced by melibiose and raffinose.
messages: Represses the melibiose operon melREDCA in the absence of melibiose or raffinose. Binds to two binding sites at the promoter region of the operon.

224308:BSU 09930
gene id: 224308:BSU_09930
uniprot id: A0A6M4JH18
uniprot id: O07521
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the YhaM family.
messages: By mitomycin C and UV irradiation which requires RecA; probably a member of the yhaONM operon.
messages: Correct processing of the 3' end of 23S rRNA no longer occurs in the absence of mrnC.
messages: Shows a 3'-5' exoribonuclease activity as well as single-stranded DNA 3'-5'exonuclease activity. Plays a role in the secondary pathway of 23S rRNA 3' end maturation.
messages: Shows a 3'-5' exoribonuclease activity.

224308:BSU 17680
gene id: 224308:BSU_17680
uniprot id: A0A6M4JIN6
uniprot id: P0CI79
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis strain 168 possesses two thymidylate synthases, a major form ThyA and a minor form ThyB.
messages: Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
messages: Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 17630
gene id: 224308:BSU_17630
uniprot id: A0A6M3ZBG2
uniprot id: P94493
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 14410
gene id: 224308:BSU_14410
uniprot id: A0A6M3ZFL7
uniprot id: P71013
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.
messages: Belongs to the peptidase S26 family.
messages: Cell membrane
messages: Expressed at the postexponential growth phase; regulated by the DegS-DegU system.
messages: Membrane

224308:BSU 25180
gene id: 224308:BSU_25180
uniprot id: P54471
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TrmK family.
messages: Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 22 (m1A22) in tRNA.
messages: Cytoplasm

224308:BSU 13500
gene id: 224308:BSU_13500
uniprot id: A0A6M4JHY1
uniprot id: O31658
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TrkH potassium transport family. Ktr (TC 2.A.38.4) subfamily.
messages: Cell membrane
messages: Homodimer. Part of the KtrCD complex formed by an octameric catalytic ring of KtrC and a membrane associated dimer of KtrD forming a potassium channel (By similarity).
messages: Impaired potassium uptake.
messages: Integral membrane subunit of the KtrCD potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.
messages: Membrane

224308:BSU 06610
gene id: 224308:BSU_06610
uniprot id: A0A6M4JKU6
uniprot id: O34580
names: Function
names: Similarity
names: Subunit
messages: Belongs to the helicase family. UvrD subfamily.
messages: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair.
messages: Interacts with YxaL; YwhK and YerB. Interacts with the RNA polymerase core (PubMed:21710567).

224308:BSU 26590
gene id: 224308:BSU_26590
uniprot id: A0A6M3ZE73
uniprot id: P39843
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally diverse compounds as ethidium bromide, rhodamine and acridine dyes, tetraphenylphosphonium, puromycin, chloramphenicol, doxorubicin, and fluoroquinolone antibiotics.
messages: Membrane

224308:BSU 03590
gene id: 224308:BSU_03590
uniprot id: A0A6M4JCS1
uniprot id: P39456
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. L-cystine importer (TC 3.A.1.3.14) family.
messages: Cell membrane
messages: Part of the ABC transporter complex TcyABC involved in L-cystine import. Responsible for energy coupling to the transport system (Probable).
messages: The complex is composed of two ATP-binding proteins (TcyC), two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).

224308:BSU 03430
gene id: 224308:BSU_03430
uniprot id: P12667
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed. Degrades both double-stranded, linear and covalently closed circular DNA.
messages: Cell membrane
messages: Mn(2+) ion stimulates activity.
messages: The activity can be inhibited by the 18 kDa competence-specific protein nin.
messages: This protein is a subunit of a 75 kDa protein complex, which governs binding and entry of donor DNA. The complex is a tetramer of two subunits of the DNA-entry nuclease and two subunits of a competence-specific protein. Only the complex is able to bind ds- and ss-DNA.
messages: To B.subtilis NucB.

224308:BSU 16930
gene id: 224308:BSU_16930
uniprot id: A0A6M3ZAZ2
uniprot id: P46323
names: Similarity
messages: Belongs to the CinA family.

224308:BSU 36320
gene id: 224308:BSU_36320
uniprot id: P94589
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Interacts with both the D1 and D2 domains of dynamin-like protein DynA.
messages: Only expressed during growth in minimal medium (PubMed:14762004).

224308:BSU 01620
gene id: 224308:BSU_01620
uniprot id: A0A6M4JHW6
uniprot id: P40410
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Induced by Btr in iron-limited conditions.
messages: Involved in the uptake of iron. Probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Membrane raft
messages: Part of the ABC transporter complex FeuABC/YusV involved in import of the catecholate siderophores bacillibactin and enterobactin.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (FeuB and FeuC) and a solute-binding protein (FeuA).

224308:BSU 06010
gene id: 224308:BSU_06010
uniprot id: O05525
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase U48 family.
messages: Cell membrane
messages: May function as endopeptidase which proteolytically removes the C-terminal three residues of farnesylated peptides containing the CAAX motif where C is cysteine, A is an aliphatic amino acid and X is any amino acid.

224308:BSU 16560
gene id: 224308:BSU_16560
uniprot id: A0A6M4JIR2
uniprot id: O31754
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M50B family.
messages: Cell membrane
messages: Is responsible for site-2 cleavage of the RsiW anti-sigma factor (PubMed:15130127). This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor (PubMed:16899079). Can also cleave liberated signal peptides of PenP and Mpr, probably within in the cell membrane (PubMed:21810987).
messages: Membrane
messages: No cleavage of signal peptides.
messages: Regulated intramembrane proteolysis (RIP) occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. A membrane-spanning regulatory substrate protein is first cut extracytoplasmically (site-1 protease, S1P), then within the membrane itself (site-2 protease, S2P, this enzyme), while cytoplasmic proteases finish degrading the regulatory protein, liberating the effector protein.

224308:BSU 19170
gene id: 224308:BSU_19170
uniprot id: A0A6M4JH58
uniprot id: O35046
names: Similarity
messages: Belongs to the peptidase S66 family.

224308:BSU 08340
gene id: 224308:BSU_08340
uniprot id: P94443
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Negative transcriptional regulator of padC phenolic acid decarboxylase.
messages: Phenolic acids regulate PadR repression activity, by impairing it's binding to the padC promoter.

224308:BSU 35900
gene id: 224308:BSU_35900
uniprot id: P96736
names: Disruption Phenotype
names: Function
messages: Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid) from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for PGA synthesis.
messages: Cells do not produce PGA.

224308:BSU 36410
gene id: 224308:BSU_36410
uniprot id: A0A6M4JPY0
uniprot id: P39751
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis has three MreB paralogs: MreB, Mbl and MreBH. All paralogs have the ability to support rod-shaped cell growth normal conditions. The multiplicity of paralogs becomes important under stress conditions. They are probably used to allow cells to maintain proper growth and morphogenesis under changing conditions.
messages: Belongs to the FtsA/MreB family.
messages: Cytoplasm
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination (PubMed:11290328, PubMed:19659933). Acts by regulating cell wall synthesis and cell elongation, and thus cell shape (PubMed:19659933). A feedback loop between cell geometry and Mbl localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature (By similarity). Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on Mbl polymerization (PubMed:21636745, PubMed:21636744). Organizes peptidoglycan synthesis in the lateral cell wall (PubMed:19659933). Also required for proper chromosome segregation (PubMed:14588250).
messages: Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.
messages: Forms polymers (PubMed:11290328, PubMed:19659933). Forms a complex with MreB and MreBH (PubMed:17064365, PubMed:19659933). Interacts with MreC (PubMed:17064365).
messages: Forms polymers.
messages: Mutants are bent and twisted at irregular angles (PubMed:11290328). Depletion leads to a strong defect in chromosome segregation (PubMed:14588250).

224308:BSU 12500
gene id: 224308:BSU_12500
uniprot id: P39780
names: Similarity
messages: To B.subtilis YqaB.

224308:BSU 33950
gene id: 224308:BSU_33950
uniprot id: A0A6M3ZGQ5
uniprot id: O32253
names: Activity Regulation
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the SorC transcriptional regulatory family.
messages: Contains an N-terminal DNA-binding domain and a large C-terminal effector-binding domain. Contains two distinct sugar-binding sites with different affinities.
messages: Homotetramer. Binds primarily as a dimer to each half-site of the full-length operator, with much higher affinity for the right site. Then, both dimers interact, bridging the two-half sites of the operator region.
messages: In the absence of glucose, represses the transcription of the gapA operon, which encodes five key glycolytic enzymes. Binds specifically to the cggR-gapA promoter region and blocks the progression of the RNA polymerase, leading to the arrest of the transcription.
messages: Stability and function are regulated by the effector molecule fructose-1,6-bisphosphate (FBP). In the presence of glucose, binding of FBP to the low-affinity sugar-binding site of CggR disrupts dimer/dimer bridging interactions and triggers a tetramer to dimer transition, which leaves two physically independent dimers on the target DNA and allows transcription of the downstream coding sequences by the RNA polymerase. In addition, FBP and several other phosphorylated compounds can bind to a high-affinity binding-site and protect CggR against aggregation and proteolysis.

224308:BSU 24640
gene id: 224308:BSU_24640
uniprot id: P40949
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Mutation impairs colony surface architecture (PubMed:16430695). Mutant produces fewer and altered amyloid fibers (PubMed:21477127).
messages: Part of the tapA-sipW-tasA operon (PubMed:10464223). Expression is directly repressed by the DNA-binding protein master regulator of biofilm formation SinR and activated by the extracellular matrix regulatory protein RemA (PubMed:16430695, PubMed:23646920). Also positively regulated by the sporulation transcription factors sigma H and Spo0A and repressed by the transition phase regulatory protein AbrB, probably indirectly (PubMed:10464223). Induced by a high concentration of salt (PubMed:10559173). During most conditions of growth, may be present at very low levels or is not synthesized at all, due, at least in part, to post-transcriptional repression (PubMed:10559173).
messages: Required for biofilm formation (PubMed:16430695, PubMed:16430696, PubMed:21477127, PubMed:24488317). Required for the proper anchoring and polymerization of TasA amyloid fibers at the cell surface (PubMed:16430696, PubMed:21477127, PubMed:24488317). Is also a minor component of TasA fibers (PubMed:21477127).
messages: Secreted
messages: cell wall

224308:BSU 28350
gene id: 224308:BSU_28350
uniprot id: A0A6M4JK39
uniprot id: P94559
names: Similarity
messages: Belongs to the metallophosphoesterase superfamily. YfcE family.

224308:BSU 34740
gene id: 224308:BSU_34740
uniprot id: A0A6M3ZGN2
uniprot id: O06976
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Along with seryl-phosphorylated HPr, phosphorylated Crh is implicated in carbon catabolite repression (CCR) of levanase, inositol dehydrogenase, and beta-xylosidase. Exerts its effect on CCR by interacting with CcpA.
messages: Belongs to the HPr family.
messages: Mixture of monomers and homodimers. Interacts with CcpA as a monomer.
messages: Not phosphorylated by PEP and by enzyme I.

224308:BSU 32130
gene id: 224308:BSU_32130
uniprot id: A0A6M3ZFZ0
uniprot id: O05269
names: Function
names: Induction
names: Similarity
messages: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.
messages: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides (Probable).
messages: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.
messages: Expression is regulated by PurR.

224308:BSU 03600
gene id: 224308:BSU_03600
uniprot id: A0A6M4JCW7
uniprot id: P42200
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex TcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (TcyC), two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).

224308:BSU 04220
gene id: 224308:BSU_04220
uniprot id: P80238
names: Induction
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 13780
gene id: 224308:BSU_13780
uniprot id: O31681
names: Developmental Stage
names: Domain
names: Miscellaneous
names: Subcellular Location Annotation
messages: Expressed in the mother cell compartment from T5 of sporulation.
messages: Expression of ykvP is regulated by SigK and dependent on GerE.
messages: Inactivation of the ykvP gene does not affect vegetative growth, spore resistance to heat, chloroform and lysozyme, or spore germination in the presence of L-alanine.
messages: LysM domains are thought to be involved in peptidoglycan binding.
messages: Spore wall

224308:BSU 19749
gene id: 224308:BSU_19749
uniprot id: A0A6M3ZCA0
uniprot id: C0H435
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Membrane

224308:BSU 12480
gene id: 224308:BSU_12480
uniprot id: O34785
names: Similarity
messages: Belongs to the UPF0714 family.

224308:BSU 38140
gene id: 224308:BSU_38140
uniprot id: A0A6M3ZHX9
uniprot id: P34959
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome c oxidase bacterial subunit 4 family.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water. Major component for energy conversion during vegetative growth (By similarity).
messages: Cell membrane
messages: Membrane

224308:BSU 28510
gene id: 224308:BSU_28510
uniprot id: A0A6M3ZF61
uniprot id: P94552
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 51 family.
messages: Cells lacking this gene retain 70% of arabinofuranosidase activity, and the double mutant abfA/abf2 is inactive.
messages: Cytoplasm
messages: Homohexamer; trimer of dimers.
messages: Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-L-arabinofuranosyl residues in different hemicellulosic homopolysaccharides (branched and debranched arabinans) and heteropolysaccharides (arabinoxylans). It is able to hydrolyze the alpha-(1->5)-glycosidic linkages of linear alpha-(1->5)-L-arabinan (debranched), sugar beet arabinan (branched) and wheat arabinoxylan. Moreover, it displays higher activity towards branched arabinan, a molecule comprising a backbone of alpha-(1->5)-linked L-arabinofuranosyl residues decorated with alpha-(1->2)-, and alpha-(1->3)-linked L-arabinofuranosyl units, than towards debranched arabinan. In addition, arabinoxylan, which has L-arabinofuranose residues attached to the main chain by alpha-(1->2)- and/or alpha-(1->3)-glycosidic linkages, is preferred to linear alpha-(1->5)-L-arabinan.
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 35620
gene id: 224308:BSU_35620
uniprot id: Q02114
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are cell wall hydrolases involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teichoic acid-endowed peptidoglycan. LytC is required for efficient swarming motility but not at the level of cell separation or flagellum biosynthesis. Rather, LytC appears to be important for proper flagellar function.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
messages: Expressed under the control of SigD (major), the transcripts being predominants at the exponential growth phase, and SigA (minor). Repressed by LytR and YvrH.
messages: Inactivation of this gene leads to an approximately 90% decrease in the total cell wall hydrolytic activity of stationary-phase cells and extraordinary resistance to cell lysis, even after 6 days of incubation at 37 degrees Celsius. Cells from domesticated laboratory strains lacking this gene show no motility changes on swarm plates; however in combination with an acetylglucosaminidase deletion (lytD, AC P39848) greatly reduced motility is seen. They also show no apparent changes in cell morphology, competence, sporulation, or germination. Cells from an undomesticated strain (3610) lacking this gene show a reduction in the rate of swarming motility.
messages: cell wall

224308:BSU 11220
gene id: 224308:BSU_11220
uniprot id: A0A6M4JKE8
uniprot id: P36839
names: Cofactor
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.
messages: Binds 1 pyridoxal phosphate per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.

224308:BSU 09490
gene id: 224308:BSU_09490
uniprot id: O07579
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 37990
gene id: 224308:BSU_37990
uniprot id: P39613
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27580
gene id: 224308:BSU_27580
uniprot id: O32041
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
messages: Probably involved in cell-wall metabolism.
messages: cell wall

224308:BSU 29120
gene id: 224308:BSU_29120
uniprot id: A0A6M3ZIA5
uniprot id: P49814
names: Function
names: Similarity
messages: Belongs to the LDH/MDH superfamily. LDH family.
messages: Belongs to the LDH/MDH superfamily. MDH type 3 family.
messages: Catalyzes the reversible oxidation of malate to oxaloacetate.

224308:BSU 03290
gene id: 224308:BSU_03290
uniprot id: P42436
names: Cofactor
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Associates with NasD.
messages: Binds 1 [2Fe-2S] cluster.
messages: Cytoplasm
messages: Positively regulated by TnrA under nitrogen-limited conditions. Induced by ArfM.
messages: Required for nitrite assimilation. Required for activity of the reductase (By similarity).

224308:BSU 07020
gene id: 224308:BSU_07020
uniprot id: O31523
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Almost completely inhibited by diethylpyrocarbonate at 5 mM and completely inhibited by phenylmethylsulfonyl fluoride (PMSF) at 50 mM. Dimethyl phosphite achieves only a 53% inhibition. Also inhibited by metal ions (magnesium, manganese and calcium) and chelating agent (EDTA) at the same level.
messages: Belongs to the 'GDSL' lipolytic enzyme family.
messages: May play a role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also active toward acetylated xylan.
messages: Monomer.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 11190
gene id: 224308:BSU_11190
uniprot id: A0A6M3ZCV8
uniprot id: P23715
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.
messages: Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.
messages: Cytoplasm

224308:BSU 10990
gene id: 224308:BSU_10990
uniprot id: O06743
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 21480
gene id: 224308:BSU_21480
uniprot id: P68577
names: Caution
names: Disruption Phenotype
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Bacteriocin active against Gram-positive bacteria. Inhibits B.cereus spore outgrowth, after the germination stage, approximately 1000-fold better than it inhibits exponential growth of the same cells. Inhibits B.subtilis strain ATCC 6633.
messages: Loss of bacteriocin sublancin 168 production, but not resistance to sublancin.
messages: Monomer.
messages: Production of active sublancin-168 requires at least one thiol-disulfide oxidoreductase (BdbB or, in its absence, BdbC). Membrane translocation and cleavage of the precursor are probably performed by SunT.
messages: Secreted
messages: Was originally (PubMed:9722542, PubMed:11872755) thought to be a lantibiotic but was later shown to be an S-linked glycopeptide.

224308:BSU 30460
gene id: 224308:BSU_30460
uniprot id: O34712
names: Developmental Stage
names: Function
messages: Expressed early in the stationary phase.
messages: Negatively regulates ABC transporter complex ytrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.

224308:BSU 01290
gene id: 224308:BSU_01290
uniprot id: A0A6M3Z6V1
uniprot id: P12878
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.
messages: Cannot be disrupted; as the protein decreases in cells 70S ribosome formation decreases rapidly, with concomitant loss of uS2, uS3 and YugI (general stress protein 13) proteins from the 30S subunit.
messages: Part of the 30S ribosomal subunit (PubMed:30126986, PubMed:17163968). Contacts proteins S3 and S10 (By similarity).
messages: Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.
messages: The major uS14 protein in the ribosome. Required for binding of uS2 and uS3 to the 30S subunit and for association of the 30S with the 50S subunit.

224308:BSU 05680
gene id: 224308:BSU_05680
uniprot id: A0A6M4JFU3
uniprot id: P96709
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Bcr/CmlA family.
messages: Cell membrane
messages: Membrane

224308:BSU 06240
gene id: 224308:BSU_06240
uniprot id: A0A6M3Z863
uniprot id: O34788
names: Cofactor
names: Disruption Phenotype
names: Similarity
names: Subunit
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Binds 1 Zn(2+) per subunit.
messages: Cells lacking this gene have no acetoin reductase/2,3-butanediol dehydrogenase activity.
messages: Homotetramer (Ref.5). Interacts with BrxC (PubMed:33722570).

224308:BSU 20040
gene id: 224308:BSU_20040
uniprot id: O30601
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the ribonucleoside diphosphate reductase small chain family.
messages: Binds 2 iron ions per subunit.
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
messages: Tetramer of two alpha and two beta subunits.
messages: This gene has an intron within which is encoded YosQ, a putative homing endonuclease.

224308:BSU 16200
gene id: 224308:BSU_16200
uniprot id: A0A6M4JGP9
uniprot id: P24502
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliE family.

224308:BSU 08470
gene id: 224308:BSU_08470
uniprot id: O31570
names: Similarity
messages: Belongs to the PhzF family.

224308:BSU 36160
gene id: 224308:BSU_36160
uniprot id: A0A6M3ZJU6
uniprot id: P96725
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LysR transcriptional regulatory family.
messages: Cytoplasm

224308:BSU 24240
gene id: 224308:BSU_24240
uniprot id: A0A6M3ZE27
uniprot id: P17894
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RecN family.
messages: Forms multimers, possibly octamers; these become larger following DNA damage. Recruited to foci following DNA damage; probably interacts with RecF and RecO.
messages: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by RecO and then RecF.
messages: May be involved in recombinational repair of damaged DNA.
messages: nucleoid

224308:BSU 30650
gene id: 224308:BSU_30650
uniprot id: A0A6M3ZFB9
uniprot id: P80879
names: Induction
names: Similarity
messages: Belongs to the Dps family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 14550
gene id: 224308:BSU_14550
uniprot id: Q45494
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.

224308:BSU 09500
gene id: 224308:BSU_09500
uniprot id: O07580
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Cotranscribed with sigma-M and yhdL. YhdK and YhdL negatively regulate sigma-M.
messages: Interacts specifically with YhdL, while the N-terminus of YhdL interacts with sigma-M.

224308:BSU 14730
gene id: 224308:BSU_14730
uniprot id: A0A6M3ZAD5
uniprot id: O07627
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: By hydrogen peroxide.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor contributes to oxidative stress resistance.
messages: The interaction between YlaC and YlaD may be regulated by the redox state of YlaD.

224308:BSU 05960
gene id: 224308:BSU_05960
uniprot id: A0A6M3ZD94
uniprot id: O05520
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MoaC family.
messages: Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).
messages: Homohexamer; trimer of dimers.

224308:BSU 10140
gene id: 224308:BSU_10140
uniprot id: A0A6M4JH04
uniprot id: P32397
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Coproporphyrinogen III oxidase subfamily.
messages: Binds 1 FAD per subunit.
messages: Cell membrane
messages: Cytoplasm
messages: Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:7928957, PubMed:9217019). Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III (PubMed:8288631, PubMed:7928957, PubMed:9217019, PubMed:9784236). Can also oxidize protoporphyrinogen IX to protoporphyrin-IX (PubMed:8288631, PubMed:7928957, PubMed:9217019, PubMed:9784236, PubMed:19944166). The specific activity for the oxidation of coproporphyrinogen III is much higher than that for the oxidation of protoporphyrinogen IX (PubMed:7928957, PubMed:9217019). Can also oxidize mesoporphyrinogen IX, but not uroporphyrinogen III (PubMed:8288631, PubMed:9784236, PubMed:7928957).
messages: Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III.
messages: Monomer.
messages: Mutations cause the accumulation of coproporphyrinogen III or coproporphyrin III in the growth medium and the accumulation of trace amounts of other porphyrinogens or porphyrins intracellularly.
messages: Only weakly inhibited by acifluorfen, in contrast to eukaryotic family members (PubMed:8288631, PubMed:9784236, PubMed:19944166). Weakly inhibited by methylacifluorfen (PubMed:9784236). Bilirubin, biliverdin and hemin are all competitive inhibitors (PubMed:9784236).

224308:BSU 06450
gene id: 224308:BSU_06450
uniprot id: A0A6M4JDP7
uniprot id: P12046
names: Similarity
messages: Belongs to the SAICAR synthetase family.

224308:BSU 30090
gene id: 224308:BSU_30090
uniprot id: O34378
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 14300
gene id: 224308:BSU_14300
uniprot id: O31705
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MoaE family.
messages: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD (By similarity).
messages: Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis (By similarity).

224308:BSU 24090
gene id: 224308:BSU_24090
uniprot id: A0A6M3ZH16
uniprot id: P54530
names: Function
names: Similarity
messages: Belongs to the phosphate acetyltransferase and butyryltransferase family.
messages: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate.

224308:BSU 24280
gene id: 224308:BSU_24280
uniprot id: A0A6M3ZH31
uniprot id: P54383
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FPP/GGPP synthase family.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Cytoplasm

224308:BSU 33620
gene id: 224308:BSU_33620
uniprot id: O32232
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the lipase/esterase LIP3/BchO family.
messages: Constitutively expressed, part of a 5 gene operon with multiple promoters. Not ethanol-stress induced.
messages: Homodimer.
messages: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones (By similarity).
messages: No visible phenotype.

224308:BSU 38110
gene id: 224308:BSU_38110
uniprot id: A0A6M3ZHL9
uniprot id: P39605
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the flavin oxidoreductase frp family.
messages: Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism. NADPH is more effective as an electron donor than NADH.
messages: Homodimer.
messages: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes.

224308:BSU 16690
gene id: 224308:BSU_16690
uniprot id: A0A6M4JGU2
uniprot id: P50849
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the polyribonucleotide nucleotidyltransferase family.
messages: Cytoplasm
messages: Homodimer (Probable). Component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno (although rnjA and rnjB's presence is unclear) (PubMed:19193632). RNA helicase CshA may also be a member of this complex (PubMed:20572937).
messages: Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.
messages: Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction. Necessary for competence development in Bacillus subtilis. May be necessary for modification of the srfA transcript (stabilization or translation activation). Involved in processing precursor type I toxin-antitoxin RNAs antitoxin SR4 and SR5 RNAs to their mature forms (PubMed:22229825, PubMed:26940229).
messages: Not essential, doubling time increased slightly. About 800-fold less compentent for plasmid transformation, no effect on sporulation efficiency. Grows poorly at 18 degrees Celsius. Increased sensitivity to several translation inhibiting antibiotics such as tetracycline, erythromycin and chloramphenicol, but increased resistance to streptomycin and nalidixic acid. Forms long filamentous cells, probably due to defective septum formation, cell walls are altered with looser, less dense peptidoglycan. Double pnp-rny mutants grow very slowly, while pnp-rnjA mutants could not be isolated (PubMed:23504012). Accumulation of precursor forms of type I toxin-antitoxin system antitoxin RNAs SR4 and SR5 (PubMed:22229825, PubMed:26940229).

224308:BSU 02870
gene id: 224308:BSU_02870
uniprot id: A0A6M3Z760
uniprot id: O34610
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-3 integral membrane protein family.
messages: Cell membrane
messages: Disruption results in low transformability.
messages: Membrane
messages: Part of the high-affinity ABC transporter complex ZnuABC involved in zinc import (Probable). Responsible for the translocation of the substrate across the membrane (By similarity). ZnuABC-mediated zinc transport is required for comF expression and competence development.
messages: Repressed by zinc via the metallo-regulatory protein Zur.
messages: The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA).

224308:BSU 02600
gene id: 224308:BSU_02600
uniprot id: P42249
names: Activity Regulation
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Activated, directly or indirectly, by endogenous spore dipicolinic acid released in response to nutrients. Is also activated by exogenous Ca(2+)-dipicolinic acid.
messages: Belongs to the CwlJ family.
messages: Expressed during sporulation; in mother cell compartment, then located in the spore coat.
messages: Expression is sigma E-dependent.
messages: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination.
messages: Secreted

224308:BSU 11040
gene id: 224308:BSU_11040
uniprot id: O06748
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 13370
gene id: 224308:BSU_13370
uniprot id: O35040
names: Cofactor
names: Similarity
messages: Belongs to the metallophosphoesterase superfamily.
messages: Binds 2 divalent metal cations.

224308:BSU 12280
gene id: 224308:BSU_12280
uniprot id: O34633
names: Induction
messages: Transcriptionally regulated by sigma-A factor. Down-regulated by rex. Highest expression during the exponential growth phase.

224308:BSU 39750
gene id: 224308:BSU_39750
uniprot id: A0A6M3ZIW7
uniprot id: P42413
names: Function
names: Similarity
messages: Belongs to the isomerase IolB family.
messages: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to 5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D-gluconate).

224308:BSU 11810
gene id: 224308:BSU_11810
uniprot id: O31625
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Transcription factor involved in spore coat assembly and spore resistance. Regulates the transcription of at least cgeA, cotG and cotS.

224308:BSU 26800
gene id: 224308:BSU_26800
uniprot id: O05413
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the nitronate monooxygenase family. NMO class I subfamily.
messages: Binds 1 FMN per subunit.
messages: Nitronate monooxygenase that uses molecular oxygen to catalyze the oxidative denitrification of alkyl nitronates. Acts on propionate 3-nitronate (P3N), the presumed physiological substrate. Probably functions in the detoxification of P3N, a metabolic poison produced by plants and fungi as a defense mechanism.
messages: P3N is a potent irreversible inhibitor of the key enzyme succinate dehydrogenase in the Krebs cycle and electron transport chain. P3N has been shown to be a toxic metabolite to bacteria, plants, fungi, mammals or any organism that uses succinate dehydrogenase.

224308:BSU 05550
gene id: 224308:BSU_05550
uniprot id: A0A6M3ZD51
uniprot id: P96698
names: Induction
names: Similarity
messages: Belongs to the small heat shock protein (HSP20) family.
messages: Induced late during sporulation by the sporulation-specific sigma factor sigma-K and negatively regulated by GerE.

224308:BSU 15550
gene id: 224308:BSU_15550
uniprot id: A0A6M4JIJ4
uniprot id: P25971
names: Function
names: Similarity
names: Subunit
messages: Belongs to the OMP decarboxylase family. Type 1 subfamily.
messages: Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).
messages: Homodimer.

224308:BSU 27785
gene id: 224308:BSU_27785
uniprot id: O32057
names: Similarity
messages: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

224308:BSU 23090
gene id: 224308:BSU_23090
uniprot id: P35166
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Insertion mutations in this gene partially restore antibiotic resistance to the beta-lactam antibiotic cefuroxime (CEF) in a sigM deletion mutant (PubMed:22211522).
messages: The anti-sigma factor for extracytoplasmic function (ECF) sigma factor SigX, inhibits SigX activity and stabilizes it.

224308:BSU 17390
gene id: 224308:BSU_17390
uniprot id: A0A6M4JIK1
uniprot id: P50621
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ribonucleoside diphosphate reductase small chain family.
messages: Binds 2 iron ions per subunit.
messages: Essential, at least the last 3 genes of the locus cannot be deleted; could be due to polar effects on downstream ymaB.
messages: Part of the probable nrdI(ymaA)-nrdE-nrdF-ymaB operon. Expression is constitutive but low, dramatically induced by thymidine starvation which requires recA.
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
messages: Tetramer of two alpha and two beta subunits.

224308:BSU 25440
gene id: 224308:BSU_25440
uniprot id: A0A6M4JNQ3
uniprot id: P54461
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RNA methyltransferase RsmE family.
messages: Cytoplasm
messages: Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity).
messages: Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.

224308:BSU 09450
gene id: 224308:BSU_09450
uniprot id: O07575
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 11090
gene id: 224308:BSU_11090
uniprot id: O06753
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05330
gene id: 224308:BSU_05330
uniprot id: P96677
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
messages: Can also bind zinc in vitro, but it does not impair DNA binding.
messages: Cytoplasm
messages: Metal-responsive transcriptional regulator that represses transcription of the aseR-ydfA operon by binding specifically to its promoter. Binding of arsenite or antimonite causes the repressor to dissociate from the DNA.

224308:BSU 13220
gene id: 224308:BSU_13220
uniprot id: A0A6M4JJ73
uniprot id: O34362
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YkoCDEF that could transport hydroxymethylpyrimidine (HMP) and/or thiamine. Could also transport other HMP-containing products. Responsible for energy coupling to the transport system (Probable).
messages: The complex is composed of two ATP-binding proteins (YkoD), two transmembrane proteins (YkoC and YkoE) and a solute-binding protein (YkoF).

224308:BSU 36950
gene id: 224308:BSU_36950
uniprot id: A0A6M4JQ45
uniprot id: P39153
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SUA5 family.
messages: Complements a S.cerevisiae SUA5 disruption mutant.
messages: Cytoplasm
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Is also able to catalyze the reverse reaction in vitro, i.e. the formation of ATP from TC-AMP and PPi.
messages: The four proteins YwlC, TsaD, TsaB and TsaE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine biosynthesis in vitro in B.subtilis.
messages: The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in B.subtilis and many other species (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA.
messages: Unlike previously thought, the formation of TC-AMP does not proceed via an ATP-activated HCO(3)(-) intermediate such as carboxy-AMP.

224308:BSU 36190
gene id: 224308:BSU_36190
uniprot id: A0A6M4JPX6
uniprot id: P96722
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Probably interacts with cognate immunity protein YwqK but not with non-cognate immunity proteins. The interaction inhibits the toxic activity of YwqJ.
messages: Secreted
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis; no effect is seen on DNA or rRNA. Co-overexpression with cognate immunity protein YwqK does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation (PubMed:34280190). Its toxic effects are probably neutralized by its cognate immunity protein YwqK, but not by immunity proteins specific to other toxins with the LXG domain (Probable). May have deaminase activity (Probable).

224308:BSU 11500
gene id: 224308:BSU_11500
uniprot id: A0A6M4JHC4
uniprot id: O31602
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ArsC family. Spx subfamily.
messages: Cytoplasm
messages: Global transcriptional regulator that plays a key role in stress response and exerts either positive or negative regulation of genes (PubMed:12642660, PubMed:15659166, PubMed:16885442, PubMed:18662407, PubMed:18687074, PubMed:29271514). Acts by interacting with the C-terminal domain of the alpha subunit of the RNA polymerase (RNAP) (PubMed:12642660, PubMed:15659166, PubMed:18687074, PubMed:20084284). This interaction can enhance binding of RNAP to the promoter region of target genes and stimulate their transcription, or block interaction of RNAP with activator proteins and repress transcription (PubMed:12642660, PubMed:15659166, PubMed:18687074, PubMed:20084284). Exhibits no DNA-binding activity (PubMed:15659166, PubMed:18687074).
messages: Global transcriptional regulator that plays a key role in stress response and exerts either positive or negative regulation of genes. Acts by interacting with the C-terminal domain of the alpha subunit of the RNA polymerase (RNAP). This interaction can enhance binding of RNAP to the promoter region of target genes and stimulate their transcription, or block interaction of RNAP with activator.
messages: Inactivation of the gene results in ClpP-independent competence development as well as partial suppression of the sporulation defect conferred by clpP mutation (PubMed:11703662). Null mutant shows hypersensitivity to disulfide stress and to paraquat (PubMed:14597697, PubMed:18662407). Mutants have increased sensitivity toward cell wall active antibiotics inhibiting both early and late steps in peptidoglycan synthesis (PubMed:29271514). Cells lacking the gene are defective in thermotolerance (PubMed:24417481).
messages: Induces the expression of a large number of genes in response to a variety of stress conditions, such as disulfide, heat and cell wall stress, while concurrently repressing transcription of genes involved in various developmental and growth-related pathways during periods of extreme stress (PubMed:12642660, PubMed:14597697). Functions in the oxidative stress response via induction of the transcription of thioredoxin (trxA) and thioredoxin reductase (trxB) during thiol-specific oxidative (disulfide) stress (PubMed:14597697, PubMed:15659166, PubMed:18687074). Mediates response to oxidative stress caused by paraquat (PQ) via induction of the methionine sulfoxide reductase genes, msrA and msrB (PubMed:18662407). Also acts as a transcriptional activator of the bacillithiol (BSH) biosynthesis genes in response to oxidizing conditions and thio-reactive compounds (PubMed:23894131). Involved in heat stress response and thermotolerance development, which results in diminished cellular protein aggregates (PubMed:24417481). Plays an important adaptive role in the cell wall stress response (PubMed:29271514). Participates in sulfate-dependent control of organosulfur metabolism. Negatively controls, via CymR, the expression of the organosulfur utilization operons ytmI, yxeI and ssu, and directly activates yrrT operon expression during growth in medium containing methionine as sole sulfur source (PubMed:16885442). Negatively affects competence and sporulation (PubMed:11703662, PubMed:12028382). Inhibits biofilm formation in response to disulfide stress by repressing biofilm matrix genes (PubMed:30718304).
messages: Interacts with the C-terminal domain of the alpha subunit of the RNAP (PubMed:12642660, PubMed:16249335, PubMed:19580872, PubMed:20084284, PubMed:22307755). A single Spx monomer interacts with RNAP to form the transcription activation complex (PubMed:22307755). Interacts with the adapter protein SpxH/YjbH (PubMed:19074380, PubMed:24942655, PubMed:30982633).
messages: Interacts with the C-terminal domain of the alpha subunit of the RNAP.
messages: The C-terminal region is essential for structural folding and for interaction with SpxH/YjbH (PubMed:24942655). A conformational change during oxidation of Spx to the disulfide form likely alters the structure of Spx alpha helix alpha4, which contains residues that function in transcriptional activation and Spx/RNAP-promoter interaction (PubMed:20084284).
messages: Transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic region (PubMed:17158660, PubMed:29271514). Induced by heat, salt, ethanol and disulfide stress and also by phosphate limitation (PubMed:11544224, PubMed:14597697, PubMed:24417481). Induced by cell wall stress but not by membrane stress (PubMed:29271514). Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969, PubMed:29271514). Repressed by YodB and PerR. YodB protects a region that includes the P3 -10 and -35 regions, while PerR binds to a region downstream of the P3 transcriptional start site (PubMed:17158660).
messages: Under non-stress conditions, Spx is degraded by ClpXP and, to a lesser extent, by ClpCP (PubMed:12057962, PubMed:12642660, PubMed:19074380). Efficient dedradation by ClpXP requires the adapter protein SpxH/YjbH (PubMed:17908206, PubMed:19074380). Binding to SpxH/YjbH reduces the overall conformational flexibility of Spx and stabilizes the C-terminal ClpX recognition region of Spx (PubMed:30982633). In addition, activity is modulated by the formation of a disulfide bound within the N-terminal Cys-X-X-Cys (CXXC) motif, which is required for the transcriptional activation of trxA and trxB, or for the activation of msrAB operon expression following paraquat oxidative stress (PubMed:15659166, PubMed:18662407). However, it seems that formation of the disulfide bound is not essential for induction of all Spx-controlled genes, as for example the case of BSH biosynthesis genes (PubMed:23894131). Similarly, induction of the Spx regulon during cell wall stress is not accompanied by oxidation of the disulfide switch, but requires Spx stabilization by the anti-adapter protein SpxO/YirB (PubMed:29271514, PubMed:30001325).

224308:BSU 28810
gene id: 224308:BSU_28810
uniprot id: A0A6M3ZJV7
uniprot id: P94522
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 43 family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Cells lacking this gene retains about 40% of the capacity to hydrolyze linear arabinan compared to the wild-type.
messages: Induced by arabinose and arabina,n and repressed by glucose.
messages: Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of linear 1,5-alpha-L-arabinan and of branched sugar beet arabinan. It displays no activity against heavily substituted arabinans or a range of other polysaccharides (larch wood arabinogalactan, wheat arabinoxylan and p-nitrophenyl-alpha-L-arabinofuranoside). The enzyme activity is progressively reduced as alpha-(1->5)-chains become shorter or more highly substituted.
messages: Secreted

224308:BSU 10420
gene id: 224308:BSU_10420
uniprot id: P40396
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
messages: A master regulator required for the expression of late competence genes including comC, comE, comG and the bdbDC operon (PubMed:8196543, PubMed:7783616, PubMed:11744713, PubMed:11918817, PubMed:11948146). Receives signals from SrfA, and possibly other regulatory COM genes, and transduces these signals to the late COM genes (PubMed:8196543, PubMed:7783616). Represses transcription of rok (PubMed:11849533). May repress expression of a few genes (PubMed:7783616).
messages: Expressed in cells competent for DNA transformation; that is 5-10% of the population (PubMed:11918817).
messages: Positively regulates its own expression (PubMed:7783616). Expressed only in glucose-based minimal medium during the transition to stationary phase (PubMed:8196543). Expression is initiated at the time of transition to the post-exponential state, and exclusively in competence-stimulating medium. Repressed by AbrB and Rok (PubMed:11849533).
messages: Transformation deficient (PubMed:8196543). Loss of expression of at least 165 genes (PubMed:11918817, PubMed:11948146).

224308:BSU 26100
gene id: 224308:BSU_26100
uniprot id: P45925
names: Similarity
messages: To B.subtilis XkdI.

224308:BSU 32700
gene id: 224308:BSU_32700
uniprot id: A0A6M3ZGP0
uniprot id: O32165
names: Function
names: Similarity
names: Subunit
messages: Belongs to the UPF0051 (ycf24) family.
messages: Part of the SufBCD complex that contains SufB, SufC and SufD.
messages: The SufBCD complex acts synergistically with SufE to stimulate the cysteine desulfurase activity of SufS. The SufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake from extracellular iron chelators under iron limitation (By similarity).

224308:BSU 09165
gene id: 224308:BSU_09165
uniprot id: P54599
names: Caution
messages: Was initially thought to be two separate ORFs named yhcO and yhcP.

224308:BSU 30970
gene id: 224308:BSU_30970
uniprot id: A0A6M4JN84
uniprot id: P39122
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.
messages: Expressed exclusively on media containing carbon sources that allow efficient sporulation.
messages: Homotetramer.
messages: Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.

224308:BSU 31280
gene id: 224308:BSU_31280
uniprot id: A0A6M3ZG03
uniprot id: O05243
names: Similarity
messages: Belongs to the UPF0047 family.

224308:BSU 27510
gene id: 224308:BSU_27510
uniprot id: A0A6M4JLH4
uniprot id: O34599
names: Function
names: Similarity
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
messages: Catalyzes the removal of elemental sulfur from cysteine to produce alanine.

224308:BSU 19310
gene id: 224308:BSU_19310
uniprot id: A0A6M4JJ07
uniprot id: O34660
names: Disruption Phenotype
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family.
messages: No effect on vanillin degradation.

224308:BSU 13040
gene id: 224308:BSU_13040
uniprot id: A0A6M3ZF63
uniprot id: P49852
names: Caution
names: Cofactor
names: Domain
names: Function
names: Induction
names: Similarity
messages: Belongs to the globin family. Two-domain flavohemoproteins subfamily.
messages: Binds 1 FAD per subunit.
messages: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
messages: By nitric oxide, nitrite, and under oxygen-limited conditions.
messages: Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.
messages: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
messages: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress (By similarity).
messages: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 02120
gene id: 224308:BSU_02120
uniprot id: A0A6M4JCQ9
uniprot id: Q45577
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. AGT (TC 2.A.3.11) family.
messages: Cell membrane
messages: In the presence of glutamate in the medium, the expression is reduced five-fold, at high potassium concentration (5 mM) (PubMed:33481774). In the absence of glutamate, expression does not respond to the supply of potassium (PubMed:33481774).
messages: Loss of the gene confers resistance to both serine and its toxic analog serine hydroxamate (PubMed:32743959). Deletion mutant is still able to transport serine, but the deletion of the three permease-encoding genes aimA (ybeC), ybxG and bcaP results in an unprecedented resistance to serine up to 100 mM (PubMed:32743959).
messages: Major glutamate and serine transporter (PubMed:32743959, PubMed:33481774). Cannot transport threonine (PubMed:32743959). AimA is the major glutamate transporter under standard growth conditions when glutamate is not limiting in the medium (PubMed:33481774).
messages: Membrane

224308:BSU 19000
gene id: 224308:BSU_19000
uniprot id: A0A6M3ZF08
uniprot id: O34330
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Deletion of the yobL-yobK operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; not dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Interacts with cognate immunity protein YobK but not with non-cognate putative immunity protein YezG. The interaction inhibits the toxic activity of YobL.
messages: Secreted
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YobK does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation.
messages: Was originally thought to be an RNase; when the C-terminus (residues 449-600) is expressed in E.coli it has RNase, not DNase activity, and inhibits growth upon expression in E.coli. In vitro RNase activity and in vivo growth inhibition are neutralized by cognate immunity protein YobK, but not by immunity proteins specific to other LXG toxins. Mutation of His-562 to Ala leads to loss of growth inhibition and RNase activity in E.coli.

224308:BSU 40380
gene id: 224308:BSU_40380
uniprot id: Q45612
names: Disruption Phenotype
names: Domain
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homodimer. Interacts with WalK and YycH.
messages: Induction of WalR-dependent gene expression. Cell wall defect.
messages: The transmembrane region is required for the regulation of WalK activity.
messages: Together with YycH, regulates the activity of the two-component system WalR/WalK.

224308:BSU 35890
gene id: 224308:BSU_35890
uniprot id: P96737
names: Disruption Phenotype
names: Function
messages: Cells fail to secrete PGA into the medium.
messages: Required for PGA (gamma-polyglutamic acid) biosynthesis.

224308:BSU 34310
gene id: 224308:BSU_34310
uniprot id: P71056
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance.
messages: Repressed by SinR.

224308:BSU 19060
gene id: 224308:BSU_19060
uniprot id: O34376
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 17920
gene id: 224308:BSU_17920
uniprot id: O31819
names: Function
names: Induction
names: Similarity
messages: Aspartyl-phosphate phosphatase which specifically dephosphorylates the sporulation transcription factor Spo0A-P and negatively regulates the sporulation initiation pathway in order to control the proper timing of sporulation.
messages: Belongs to the spo0E family.
messages: During exponential phase and in conditions antithetical to sporulation.

224308:BSU 38600
gene id: 224308:BSU_38600
uniprot id: P46321
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Positive regulator of the licABCH operon.
messages: The regulatory activity of LicR is modulated by phosphorylation and dephosphorylation of the various LicR domains. It becomes activated via phosphoryl group transfer from PEP, EI and HPr on the two conserved histidine residues in the PRD 2 domain, whereas phosphorylation of the EIIA-like domain on His-559 by the PTS EIIB component LicB inactivates LicR (By similarity).

224308:BSU 14670
gene id: 224308:BSU_14670
uniprot id: Q45499
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the inositol monophosphatase superfamily.
messages: Hydrolyzes myo-inositol monophosphate. Catalyzes the dephosphorylation of GMP and IMP.
messages: No visible phenotype during 5 hours of growth, decreased resistance to oxidative stress caused by diamide.

224308:BSU 31400
gene id: 224308:BSU_31400
uniprot id: A0A6M3ZIY7
uniprot id: Q795M6
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
messages: Cytoplasm

224308:BSU 34680
gene id: 224308:BSU_34680
uniprot id: A0A6M3ZGY7
uniprot id: O32264
names: Similarity
messages: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

224308:BSU 29070
gene id: 224308:BSU_29070
uniprot id: C0SP79
names: Developmental Stage
names: Disruption Phenotype
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Disruption of the gene causes a 10-fold reduction in the efficiency of sporulation. Mutants divide asymmetrically but fail to complete engulfment of the forespore by the mother cell.
messages: Expressed in the mother cell during sporulation.
messages: Expression is regulated by the sporulation transcription factor sigma E.

224308:BSU 00820
gene id: 224308:BSU_00820
uniprot id: A0A6M4JC51
uniprot id: P37477
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Binds 3 Mg(2+) ions per subunit.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 16825
gene id: 224308:BSU_16825
uniprot id: O31762
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Induced by mta.
messages: Involved in secretion of bacillibactin.

224308:BSU 16670
gene id: 224308:BSU_16670
uniprot id: A0A6M3ZAW2
uniprot id: P54575
names: Function
names: Similarity
messages: Belongs to the RibF family.
messages: Belongs to the ribF family.
messages: Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.

224308:BSU 25770
gene id: 224308:BSU_25770
uniprot id: P17867
names: Function
names: Similarity
messages: In the N-terminal section; belongs to the site-specific recombinase resolvase family.
messages: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of SpoIIIC and SpoIVCB to form sigma K factor.

224308:BSU 25990
gene id: 224308:BSU_25990
uniprot id: P45934
names: Similarity
messages: To B.subtilis XkdS.

224308:BSU 02860
gene id: 224308:BSU_02860
uniprot id: O34946
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Disruption results in low transformability.
messages: Part of the high-affinity ABC transporter complex ZnuABC involved in zinc import (Probable). Responsible for energy coupling to the transport system (By similarity). ZnuABC-mediated zinc transport is required for comF expression and competence development.
messages: Repressed by zinc via the metallo-regulatory protein Zur.
messages: The complex is composed of two ATP-binding proteins (ZnuC), two transmembrane proteins (ZnuB) and a solute-binding protein (ZnuA).

224308:BSU 18500
gene id: 224308:BSU_18500
uniprot id: A0A6M4JHA5
uniprot id: P14802
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 04000
gene id: 224308:BSU_04000
uniprot id: A0A6M3ZAP4
uniprot id: P42958
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the isocitrate and isopropylmalate dehydrogenases family.
messages: Binds 1 Mg(2+) or Mn(2+) ion per subunit.
messages: Has multiple catalytic activities. Apart from catalyzing the oxidation of (+)-tartrate to oxaloglycolate, also converts meso-tartrate to D-glycerate and catalyzes the oxidative decarboxylation of D-malate to pyruvate.

224308:BSU 23080
gene id: 224308:BSU_23080
uniprot id: A0A6M4JM39
uniprot id: P35146
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the type-I 3-dehydroquinase family.
messages: Homodimer.
messages: Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 04810
gene id: 224308:BSU_04810
uniprot id: P96630
names: Function
names: Subunit
messages: Interacts with ImmR.
messages: Involved in the regulation of horizontal gene transfer through the integrative and conjugative element ICEBs1. Required for degradation of the ICEBs1 repressor protein ImmR/YdcN.

224308:BSU 30560
gene id: 224308:BSU_30560
uniprot id: A0A6M3ZFS1
uniprot id: P54418
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.
messages: Binds 1 Mn(2+) ion per subunit.
messages: Cytoplasm
messages: Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 27380
gene id: 224308:BSU_27380
uniprot id: A0A6M4JJT6
uniprot id: O34828
names: Similarity
messages: Belongs to the UPF0473 family.

224308:BSU 34720
gene id: 224308:BSU_34720
uniprot id: A0A6M3ZGW5
uniprot id: O06978
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YvcQ/YvcP.
messages: Phosphorylated by YvcQ.

224308:BSU 13550
gene id: 224308:BSU_13550
uniprot id: A0A6M4JFM4
uniprot id: O31662
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.
messages: Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.
messages: Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).
messages: Homodimer.
messages: Was originally thought to be a translation initiation factor.

224308:BSU 30690
gene id: 224308:BSU_30690
uniprot id: A0A6M4JNZ0
uniprot id: O34872
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the beta-class carbonic anhydrase family.
messages: Binds 1 zinc ion per subunit.
messages: Reversible hydration of carbon dioxide.

224308:BSU 00500
gene id: 224308:BSU_00500
uniprot id: A0A6M4JBW5
uniprot id: P14192
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.
messages: Cytoplasm
messages: Homotrimer.
messages: In the C-terminal section; belongs to the transferase hexapeptide repeat family.
messages: In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 16680
gene id: 224308:BSU_16680
uniprot id: A0A6M4JK50
uniprot id: P21473
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS15 family.
messages: Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.
messages: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA (By similarity).
messages: Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.

224308:BSU 13430
gene id: 224308:BSU_13430
uniprot id: O34908
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DedA family.
messages: Cell membrane

224308:BSU 26640
gene id: 224308:BSU_26640
uniprot id: O07085
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in potassium and divalent cation transport. Enhances the transport activity of the cation/potassium transporter CzcD.

224308:BSU 18300
gene id: 224308:BSU_18300
uniprot id: A0A6M4JH86
uniprot id: O31827
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 1 phosphopantetheine covalently.
messages: This protein is a multifunctional enzyme, able to activate and polymerize the amino acid Ile as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The activation sites for this amino acid consist of individual domains.

224308:BSU 25640
gene id: 224308:BSU_25640
uniprot id: A0A6M4JJC0
uniprot id: P54455
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NadD family.
messages: Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).
messages: Homodimer.

224308:BSU 35560
gene id: 224308:BSU_35560
uniprot id: A0A6M3ZGI0
uniprot id: O32269
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: Cell membrane
messages: Membrane
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 22230
gene id: 224308:BSU_22230
uniprot id: P50829
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.

224308:BSU 35950
gene id: 224308:BSU_35950
uniprot id: A0A6M3ZJU0
uniprot id: P36948
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. AraH/RbsC subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex RbsABC involved in ribose import. Probably responsible for the translocation of the substrate across the membrane.
messages: The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB).

224308:BSU 26490
gene id: 224308:BSU_26490
uniprot id: A0A6M4JNS2
uniprot id: P54437
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.
messages: Cell membrane
messages: Membrane

224308:BSU 25500
gene id: 224308:BSU_25500
uniprot id: A0A6M3ZEE2
uniprot id: P54304
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Although the bacteria accumulates coproporphyrinogen-III when the gene is disrupted, no oxygen-independent coproporphyrinogen-III oxidase activity or complementation have been shown. The exact role of this protein is unknown.
messages: Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.
messages: Cells lacking this gene accumulate coproporphyrinogen-III under anaerobic conditions, but show normal growth under aerobic and anaerobic conditions.
messages: Cytoplasm
messages: Might carry two S-adenosyl-L-methionine binding sites with only one binding to the iron-sulfur cluster.
messages: Part of the lepA-hemN operon; there is a strong transcriptional terminator between the 2 genes, this gene is much less transcribed. There can be further readthough downstream (PubMed:8757728, PubMed:9371469). Induced under anaerobic conditions by resDE, fnr and arfM.
messages: Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently (By similarity). Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (By similarity).
messages: Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

224308:BSU 40910
gene id: 224308:BSU_40910
uniprot id: A0A6M4JR76
uniprot id: P21468
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bS6 family.
messages: Binds together with bS18 to 16S ribosomal RNA.
messages: Part of the 30S ribosomal subunit.
messages: Strongly expressed during exponential growth, decreases 2-4-fold in stationary phase, part of the rpsF-ssbA-rpsR operon (PubMed:14762004). The operon is induced by DNA damage by mitomycin C (PubMed:14762004).

224308:BSU 02060
gene id: 224308:BSU_02060
uniprot id: A0A6M3Z6T7
uniprot id: P54425
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Deletion of the gene confers a weak resistance to growth inhibition by serine (PubMed:32743959). The deletion of the three permease-encoding genes aimA (ybeC), ybxG and bcaP results in an unprecedented resistance to serine up to 100 mM (PubMed:32743959).
messages: Membrane
messages: Probable threonine transporter (PubMed:32743959). Is also active as a minor serine permease (PubMed:32743959).

224308:BSU 06370
gene id: 224308:BSU_06370
uniprot id: A0A6M4JG57
uniprot id: O34987
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.
messages: Cell membrane
messages: Cells lacking this gene show normal growth on inosine as the purine source, normal hypoxanthine-guanine phosphoribosyltransferase activity, and are resistant to azaguanine.
messages: Expression is regulated by both purR and xptR regulons. Negatively regulated by hypoxanthine and guanine.
messages: Involved in the uptake of the purine bases hypoxanthine and guanine.
messages: Membrane

224308:BSU 09480
gene id: 224308:BSU_09480
uniprot id: A0A6M4JEI1
uniprot id: O07578
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 14960
gene id: 224308:BSU_14960
uniprot id: O34586
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 26500
gene id: 224308:BSU_26500
uniprot id: A0A6M3ZED4
uniprot id: P54436
names: Similarity
messages: Belongs to the sulfur carrier protein TusA family.

224308:BSU 20810
gene id: 224308:BSU_20810
uniprot id: O34336
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the 'phage' integrase family.
messages: Little effect on the percentage of cells with partition defects and no change in sporulation frequency. However, yopP disruption strongly increases the extent of the partitioning defects seen in codV mutant strains (by about two-fold) and, to a lesser extent, those seen in ripX and dif mutant strains.
messages: Probable recombinase that does not seem to have a role in chromosome dimer resolution per se but rather may have some facilitative role during chromosome partitioning in general.

224308:BSU 33600
gene id: 224308:BSU_33600
uniprot id: A0A6M3ZGN9
uniprot id: O32230
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SmpB family.
messages: Constitutively expressed, part of a 5 gene operon with multiple promoters. Not ethanol-stress induced.
messages: Cytoplasm
messages: Not essential for growth; loss of trans-translation (PubMed:11395451). Significantly decreased growth at 16 and 52 degrees Celsius (PubMed:17369301).
messages: Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation (By similarity). Required for trans-translation (PubMed:11395451). Probably required for sporulation; deletion of the gene for tmRNA impairs sporulation via its effect on trans-translation, and as smpB is required for trans-translation under non-stress conditions, it is also probably required during sporulation (PubMed:18673456).
messages: Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.

224308:BSU 30820
gene id: 224308:BSU_30820
uniprot id: A0A6M4JLF6
uniprot id: P23970
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the TPP enzyme family. MenD subfamily.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).
messages: Homodimer.
messages: Used to include what was called 'MenCF'.

224308:BSU 17140
gene id: 224308:BSU_17140
uniprot id: A0A6M4JM36
uniprot id: P40804
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It decarboxylates selectively the malonyl group attached on the acyl-carrier-protein AcpK (Mal-AcpK).

224308:BSU 26650
gene id: 224308:BSU_26650
uniprot id: A0A6M3ZEA6
uniprot id: O07084
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.
messages: Cell membrane
messages: Involved in divalent cation and potassium homeostasis in the cell. Catalyzes the active efflux of zinc, cadmium and cobalt, in exchange for potassium and H(+) ions.
messages: Membrane
messages: Repressed by CzrA.

224308:BSU 05900
gene id: 224308:BSU_05900
uniprot id: A0A6M3Z7W4
uniprot id: O05514
names: Caution
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the thiamine-monophosphate kinase family.
messages: Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.

224308:BSU 13640
gene id: 224308:BSU_13640
uniprot id: P05043
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subunit
messages: Aspartyl-phosphate phosphatase which specifically dephosphorylates the sporulation transcription factor Spo0A-P and negatively regulates the sporulation initiation pathway in order to control the proper timing of sporulation.
messages: Belongs to the spo0E family.
messages: Interacts with Spo0A.
messages: Mutations that block the onset of sporulation in Bacillus subtilis are called stage 0 mutations, or spo0 mutations, and at least eight genetic loci have been identified in which a mutation can result in a stage 0 phenotype. Stage 0 mutants lack the ability to form the asymmetric septum characteristic of the initiation of the sporulation process. In addition, these mutations have many pleiotropic effects on the synthesis of a variety of transcripts by minor forms of RNA polymerase in this organism.
messages: Probably degraded by FtsH, the last 14 residues seem to form the FtsH recognition site.

224308:BSU 15900
gene id: 224308:BSU_15900
uniprot id: A0A6M3ZB03
uniprot id: P71019
names: Similarity
messages: Belongs to the FabD family.
messages: Belongs to the fabD family.

224308:BSU 09280
gene id: 224308:BSU_09280
uniprot id: A0A6M4JGC2
uniprot id: P18156
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
messages: Belongs to the MIP/aquaporin (TC 1.A.8) family.
messages: Cell membrane
messages: Mediates glycerol diffusion across the cytoplasmic membrane via a pore-type mechanism.
messages: Membrane
messages: Requires glycerol 3-phosphate and the GlpP product; repressed by glucose.

224308:BSU 34250
gene id: 224308:BSU_34250
uniprot id: P71062
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial sugar transferase family.
messages: Cell membrane
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Repressed by SinR.

224308:BSU 11890
gene id: 224308:BSU_11890
uniprot id: O31633
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Acetylates the N-terminal alanine of ribosomal protein S5.
messages: Belongs to the acetyltransferase family. RimJ subfamily.
messages: Cytoplasm

224308:BSU 34090
gene id: 224308:BSU_34090
uniprot id: O07016
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 24910
gene id: 224308:BSU_24910
uniprot id: A0A6M4JJG1
uniprot id: P54490
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

224308:BSU 03770
gene id: 224308:BSU_03770
uniprot id: P94415
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Rap family.
messages: Cytoplasm
messages: Deletion of the gene increases the expression of the ComA-dependent genes rapA and rapC itself.
messages: Homodimer (PubMed:12950917). Interacts specifically with the C-terminal DNA-binding domain of ComA (PubMed:12950917, PubMed:15968044). Interacts with CSF (PubMed:12950917).
messages: Inhibited by the competence and sporulation stimulating factor (CSF), encoded by phrC, which prevents RapC-ComA interaction.
messages: Involved in the regulation of genetic competence development (PubMed:12950917, PubMed:16816200). Inhibits the activity of ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:12950917, PubMed:16816200). Acts by binding to ComA, independently of its phosphorylation state, leading to the inhibition of ComA DNA-binding activity (PubMed:12950917). Does not dephosphorylate phospho-ComA and does not affect the phosphorylation level of the ComP-ComA system (PubMed:12950917).
messages: Part of the rapC-phrC operon, which is controlled by the P1 promoter (PubMed:10464187). Transcription from the P1 promoter is activated by high cell density through the phosphorylated form of ComA (PubMed:10464187). RapC is part of an autoregulatory loop, and it negatively regulates its own expression (PubMed:10464187).

224308:BSU 14620
gene id: 224308:BSU_14620
uniprot id: P39910
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 22540
gene id: 224308:BSU_22540
uniprot id: A0A6M3ZD91
uniprot id: P46913
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome b family.
messages: Cell membrane
messages: Component of the menaquinol:cytochrome c reductase complex.
messages: Membrane
messages: The main subunits of the menaquinol:cytochrome c complex are a Rieske-type iron-sulfur protein (QcrA), a cytochrome b (QcrB) and a cytochrome c (QcrC).

224308:BSU 33280
gene id: 224308:BSU_33280
uniprot id: O35007
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.
messages: Cell membrane

224308:BSU 06230
gene id: 224308:BSU_06230
uniprot id: A0A6M3Z836
uniprot id: O34718
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Major myo-inositol uptake transporter.
messages: Membrane
messages: Negatively regulated by IolR. Induced by inositol.
messages: When iolT is inactivated, inositol uptake is almost abolished even though iolF is active.

224308:BSU 10130
gene id: 224308:BSU_10130
uniprot id: A0A6M3Z933
uniprot id: P32396
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ferrochelatase family.
messages: Cytoplasm
messages: Deletion mutant requires hemin for growth.
messages: Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:25646457, PubMed:25908396). Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III (PubMed:25646457, PubMed:25908396). It can also insert iron into protoporphyrin IX (PubMed:1459957, PubMed:8119288, PubMed:21052751, PubMed:25646457). Has weaker activity with 2,4 disulfonate, deuteroporphyrin and 2,4 hydroxyethyl (PubMed:25646457, PubMed:12761666). In vitro, can also use Zn(2+) or Cu(2+) (PubMed:8119288, PubMed:16140324, PubMed:21052751, PubMed:12761666).
messages: Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III.
messages: Monomer (PubMed:8119288). Interacts with frataxin/Fra (PubMed:25826316).
messages: Stimulated by Mg(2+) (PubMed:12761666). Inhibited by Cd(2+) (PubMed:12761666). Inhibited by N-methylmesoporphyrin (N-MeMP) and 2,4-disulfonic acid deuteroporphyrin IX (dSDP) (PubMed:10704318, PubMed:16140324, PubMed:18423489).

224308:BSU 05300
gene id: 224308:BSU_05300
uniprot id: P96674
names: Similarity
messages: Belongs to the NAD(P)H dehydrogenase (quinone) family.

224308:BSU 36940
gene id: 224308:BSU_36940
uniprot id: A0A6M4JQ43
uniprot id: P39154
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MntP (TC 9.B.29) family.
messages: Cell membrane
messages: Probably functions as a manganese efflux pump.

224308:BSU 26760
gene id: 224308:BSU_26760
uniprot id: O07081
names: Similarity
messages: Belongs to the isochorismatase family.

224308:BSU 06670
gene id: 224308:BSU_06670
uniprot id: A0A6M3Z8L6
uniprot id: O06492
names: Function
names: Similarity
names: Subunit
messages: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).
messages: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
messages: Belongs to the GatC family.
messages: Heterotrimer of A, B and C subunits.

224308:BSU 04100
gene id: 224308:BSU_04100
uniprot id: P42968
names: Function
names: Induction
messages: Induced by glucose when readily available sources of nitrogen, such as ammonia or glutamine, are scarce.
messages: Transcriptional repressor of the kip gene-containing operon.

224308:BSU 01260
gene id: 224308:BSU_01260
uniprot id: A0A6M3Z786
uniprot id: P12875
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL14 family.
messages: Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8 (By similarity).
messages: Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.

224308:BSU 38280
gene id: 224308:BSU_38280
uniprot id: A0A6M3ZIW5
uniprot id: P39595
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.
messages: Cell membrane
messages: Component of the iron transporter efeUOB/M complex composed of EfeU, EfeM and EfeB; EfeU is essential for the complex formation.
messages: Membrane
messages: Part of the iron transporter system efeUOB/M involved in iron import (Probable) (PubMed:23764491). Mediates the uptake of Fe(3+) ions, delivered by EfeM, across the cell membrane (PubMed:23764491).
messages: Severe reduction in Fe(2+) and Fe(3+) uptake and in cell growth.

224308:BSU 08020
gene id: 224308:BSU_08020
uniprot id: A0A6M3Z8K8
uniprot id: O31545
names: Similarity
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.

224308:BSU 27240
gene id: 224308:BSU_27240
uniprot id: O05395
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10060
gene id: 224308:BSU_10060
uniprot id: P55341
names: Function
messages: Unknown, does not resemble components of ABC transporters.

224308:BSU 22880
gene id: 224308:BSU_22880
uniprot id: P38494
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the bacterial ribosomal protein bS1 family.
messages: Constitutively transcribed in minimal and rich medium, in exponential and stationary phase. May not be transcribed with the upstream gene (cmk), there is a probable transcription terminator between the 2 genes.
messages: No visible effect on growth (PubMed:7704259). Decreased sporulation at 37 and 47 degrees Celsius (PubMed:14586115).
messages: Plays a role in sporulation (PubMed:14586115). Cannot be expressed in wild-type E.coli, does not complement an E.coli rpsA deletion (PubMed:7704259).

224308:BSU 16040
gene id: 224308:BSU_16040
uniprot id: A0A6M4JGB2
uniprot id: O31742
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL19 family.
messages: Part of the 50S ribosomal subunit.
messages: This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.

224308:BSU 04250
gene id: 224308:BSU_04250
uniprot id: P96582
names: Function
messages: Transcriptional regulator with a possible role in regulation of amino acid metabolism. Plays a role in the growth phase transition.

224308:BSU 30800
gene id: 224308:BSU_30800
uniprot id: A0A6M3ZIS3
uniprot id: P23966
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the enoyl-CoA hydratase/isomerase family. MenB subfamily.
messages: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: The hydrogencarbonate anion plays the same catalytic role (proton acceptor) as the side-chain carboxylate group of the essential 'Asp-185' found in actinobacteria, archaea, bacteroidetes, and deltaproteobacteria.

224308:BSU 20490
gene id: 224308:BSU_20490
uniprot id: A0A6M4JJQ5
uniprot id: O31916
names: Domain
names: Function
names: Similarity
messages: Belongs to the SOS response-associated peptidase family.
messages: Glu-106 is involved in sensing abasic sites in single-stranded DNA (ssDNA). His-163 stabilizes the abasic sites by forming a hydrogen bond with the O4' hydroxyl group.
messages: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (By similarity). May act as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity).

224308:BSU 37420
gene id: 224308:BSU_37420
uniprot id: P71006
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the peptidase M16 family.
messages: Divalent metal cations. Binds Zn(2+).
messages: Required for production of the bacteriocin subtilosin. Could catalyze some step in the processing of presubtilosin.
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 20250
gene id: 224308:BSU_20250
uniprot id: A0A6M3ZBS6
uniprot id: P68585
names: Function
names: Miscellaneous
names: Similarity
messages: A putative methylase, recognizes the double-stranded sequence 5'-GGCC-3', methylates C-?. There is no known cognate restriction enzyme.
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
messages: Encoded in the SPbeta prophage region.

224308:BSU 08000
gene id: 224308:BSU_08000
uniprot id: A0A6M4JDZ8
uniprot id: O31543
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.
messages: Cell membrane
messages: Mediates influx of magnesium ions.
messages: Membrane

224308:BSU 25240
gene id: 224308:BSU_25240
uniprot id: A0A6M3ZHA4
uniprot id: P54470
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PDRP subfamily.
messages: Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.
messages: Constitutively expressed.
messages: Lowers the level of gapB and pckA transcription threefold under both glycolytic and gluconeogenic conditions.

224308:BSU 07390
gene id: 224308:BSU_07390
uniprot id: O06474
names: Function
names: Induction
names: Miscellaneous
messages: Binds a DNA inverted repeat of the yfmPO operon.
messages: Repressor of the yfmOP operon. A mutation in yfmP leads to overexpression of yfmO, probably causing a decrease in cellular copper that is eventually responsible for a reduced copper induction of copZA.
messages: YfmP is autoregulated. There is no induction with copper or other metals.

224308:BSU 34750
gene id: 224308:BSU_34750
uniprot id: A0A6M4JPH2
uniprot id: O06975
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the WhiA family.
messages: Constitutively expressed throughout the growth phase.
messages: Involved in cell division and chromosome segregation (PubMed:24097947, PubMed:29378890). May influence the activity of FtsZ (PubMed:24097947). Binds DNA, but does not seem to function as a transcription factor (PubMed:24097947).
messages: Involved in cell division and chromosome segregation.
messages: Mutant grows slower and cells are longer compared to the wild-type strain. Mutation has a relatively mild effect on the sporulation. Mutant is sensitive to reduced FtsZ concentrations. Deletion of the gene in a zapA mutant background results in very filamentous cells that are blocked in proper Z-ring formation (PubMed:24097947). Mutant shows increased internucleoid distances (PubMed:29378890).
messages: nucleoid

224308:BSU 21660
gene id: 224308:BSU_21660
uniprot id: O32006
names: Similarity
messages: In the N-terminal section; belongs to the site-specific recombinase resolvase family.

224308:BSU 09800
gene id: 224308:BSU_09800
uniprot id: A0A6M4JEF4
uniprot id: O07542
names: Similarity
messages: Belongs to the UPF0342 family.

224308:BSU 25190
gene id: 224308:BSU_25190
uniprot id: A0A6M4JLV6
uniprot id: P24469
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Binds 1 heme c group covalently per subunit.
messages: Cell membrane
messages: Not essential for growth on minimal or rich media.

224308:BSU 17730
gene id: 224308:BSU_17730
uniprot id: A0A6M4JGQ5
uniprot id: O31806
names: Similarity
messages: Belongs to the AHA1 family.

224308:BSU 20520
gene id: 224308:BSU_20520
uniprot id: O31919
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 01920
gene id: 224308:BSU_01920
uniprot id: O31423
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: Belongs to the radical SAM superfamily.
messages: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine (PubMed:23282011). The other is coordinated via 3 cysteines and maybe direct contact with the SkfA precursor (Probable).
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Catalyzes the formation of the thioether bond required for production of the sporulation killing factor (SKF) from SkfA (PubMed:12817086). Forms the cysteine-methionine thioether bond found in SKF; the acceptor amino acid can be hydrophobic, aromatic or a small hydrophilic amino acid but not a larger hydrophilic amino acid, i.e. Met=Ala, Phe, Leu, Tyr>Asn, Ser>>Gln, Glu, Lys (PubMed:23282011). The relative position of Cys and Met in the substrate cannot be inverted, in vitro the thioether bond cannot be made in the absence of the SkfA propeptide, suggesting this is the first reaction in SKF maturation (PubMed:23282011). In vitro, in the absence of a second substrate, cleaves S-adenosyl-L-methionine into Met and 5'-dA (PubMed:23282011).
messages: Cytoplasm
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 08220
gene id: 224308:BSU_08220
uniprot id: A0A6M4JG29
uniprot id: P54719
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 23950
gene id: 224308:BSU_23950
uniprot id: P54538
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 18100
gene id: 224308:BSU_18100
uniprot id: A0A6M4JIN7
uniprot id: Q45066
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.
messages: Cell membrane
messages: Cytoplasm
messages: Heterotetramer composed of ParC and ParE.
messages: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.

224308:BSU 13840
gene id: 224308:BSU_13840
uniprot id: O31687
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioredoxin family.
messages: Mutants have spores sensitive to heat, lysozyme and chloroform, and in which the cortex is absent.
messages: Spore outer membrane
messages: StoA and ykvU are cotranscribed under the control of sigma E.
messages: Thiol-disulfide oxidoreductase with a reductive function, involved in spore cortex synthesis. It could be involved either in breaking disulfide bonds in cortex components or in proteins that are important for cortex synthesis, or in thiol/disulfide bond interchange.

224308:BSU 07270
gene id: 224308:BSU_07270
uniprot id: O06486
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the glucose-1-phosphate cytidylyltransferase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate.

224308:BSU 31380
gene id: 224308:BSU_31380
uniprot id: O32087
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 23470
gene id: 224308:BSU_23470
uniprot id: A0A6M3ZDH8
uniprot id: P10727
names: Function
names: PTM
names: Similarity
messages: Belongs to the anti-sigma-factor antagonist family.
messages: In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition.
messages: Phosphorylated by SpoIIAB on a serine residue.

224308:BSU 09610
gene id: 224308:BSU_09610
uniprot id: A0A6M3Z8Y7
uniprot id: O07591
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.
messages: Cell membrane
messages: Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.
messages: Membrane
messages: Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.

224308:BSU 41020
gene id: 224308:BSU_41020
uniprot id: A0A6M3ZIR1
uniprot id: P25811
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.
messages: Binds 1 potassium ion per subunit.
messages: Cytoplasm
messages: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.
messages: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: No visible phenotype.

224308:BSU 01610
gene id: 224308:BSU_01610
uniprot id: A0A6M3Z6T6
uniprot id: P40411
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Induced by Btr in iron-limited conditions.
messages: Involved in the uptake of iron. Probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Part of the ABC transporter complex FeuABC/YusV involved in import of the catecholate siderophores bacillibactin and enterobactin.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (FeuB and FeuC) and a solute-binding protein (FeuA).

224308:BSU 34220
gene id: 224308:BSU_34220
uniprot id: P71065
names: Function
names: Induction
names: Similarity
messages: Belongs to the polysaccharide pyruvyl transferase family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Repressed by SinR.

224308:BSU 27809
gene id: 224308:BSU_27809
uniprot id: C0H464
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02210
gene id: 224308:BSU_02210
uniprot id: A0A6M4JEZ0
uniprot id: O31448
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 33880
gene id: 224308:BSU_33880
uniprot id: O32247
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 06839
gene id: 224308:BSU_06839
uniprot id: Q59HN7
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Phr family.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably only subject to processing events at the ends of the mature peptide.
messages: Cytoplasm
messages: Decreases expression from the aprE promoter.
messages: Part of the rapH-phrH operon, which is transcribed from the SigA-driven rapH promoter (PubMed:21908671). phrH is also transcribed from a putative SigA-driven promoter embedded within the rapH gene sequence (PubMed:21908671).
messages: Secreted
messages: Signaling molecule involved the regulation of both sporulation and competence (PubMed:17581123, PubMed:21908671). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (PubMed:21908671). Acts by inhibiting RapH activity (PubMed:17581123, PubMed:21908671). Can inhibit both RapH activities, the dephosphorylation of Spo0F and the sequestration of ComA (PubMed:21908671).

224308:BSU 09860
gene id: 224308:BSU_09860
uniprot id: O07535
names: Activity Regulation
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Binds cyclic di-AMP (c-di-AMP), which may regulate the activity.
messages: Cell membrane
messages: Part of the khtSTU operon. Induced by salt stress at alkaline pH.
messages: Required for activity of the potassium/proton antiporter KhtU (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis (PubMed:24330391).
messages: The transporter is composed of the integral membrane protein KhtU and the regulatory protein KhtT.

224308:BSU 07050
gene id: 224308:BSU_07050
uniprot id: O31526
names: Cofactor
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the polysaccharide lyase 11 family.
messages: Binds 10 calcium ions (PubMed:16682770, PubMed:17449691, PubMed:19193638). The calcium may have a structural role. Requires calcium or manganese for activity (PubMed:16682770, PubMed:17449691).
messages: Monomer.
messages: Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases oligosaccharide products (PubMed:16682770, PubMed:17449691, PubMed:19193638). Degrades rhamnogalacturonan, polygalacturonic acid, pectic acid and pectin (PubMed:16682770, PubMed:17449691).
messages: Rhamnose is bound near the active site, but there are also additional rhamnose-binding sites far away from the active site, which possibly function as a carbohydrate-binding region.
messages: Secreted
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 13300
gene id: 224308:BSU_13300
uniprot id: A0A6M3ZA02
uniprot id: O34442
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acts as a magnesium transporter (PubMed:24415722). MgtE is the dominant transporter under rich-medium growth conditions, and it may provide the primary route of magnesium import in B.subtilis, while the other putative transport proteins are likely to be utilized for more-specialized growth conditions (PubMed:24415722).
messages: Acts as a magnesium transporter.
messages: Belongs to the SLC41A transporter family.
messages: Binds cyclic di-AMP (c-di-AMP), which may regulate the transporter activity.
messages: Cell membrane
messages: Deletion of the gene results in a significant growth defect under rich-medium growth conditions and in strong dependency on supplemental extracellular magnesium.
messages: Homodimer.
messages: Membrane

224308:BSU 07610
gene id: 224308:BSU_07610
uniprot id: A0A6M3Z8S1
uniprot id: P55069
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CitM (TC 2.A.11) transporter family.
messages: Cell membrane
messages: Expression is under strict control of the medium composition. Induced by citrate, via the two-component regulatory system CitT/CitS. Repressed by rapidly metabolized carbon sources like glucose, glycerol and inositol, via the carbon catabolite repression system. Is also repressed by succinate and glutamate, albeit to a lesser extent.
messages: Membrane
messages: Proton motive force-driven secondary transporter that mediates the transport of citrate complexed to Mg(2+). Cotransports at least two protons per Mg(2+)-citrate complex. Can also transport citrate in complex with Ni(2+), Mn(2+), Co(2+), and Zn(2+).
messages: The uptake activity increases with increasing Mg(2+) concentrations. Inhibited by FCCP, TCC and nigericin.

224308:BSU 09470
gene id: 224308:BSU_09470
uniprot id: A0A6M3Z8X4
uniprot id: O07577
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.
messages: Cell membrane
messages: Membrane
messages: Putative sodium-dependent transporter.

224308:BSU 24000
gene id: 224308:BSU_24000
uniprot id: A0A6M4JNM8
uniprot id: P39074
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the diacylglycerol/lipid kinase family.
messages: Binds 1 Mg(2+) ion per subunit. This ion appears to have a structural role and is required for catalytic activity.
messages: May catalyze the ATP-dependent phosphorylation of lipids other than diacylglycerol (DAG). In fact, is not able to exhibit diacylglycerol kinase activity in vitro.

224308:BSU 22010
gene id: 224308:BSU_22010
uniprot id: P54161
names: Function
messages: 5'-3' exonuclease acting preferentially on double-stranded DNA.

224308:BSU 29420
gene id: 224308:BSU_29420
uniprot id: O34308
names: Caution
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Phe-155 is present instead of the conserved Tyr which is expected to be an active site residue.

224308:BSU 04160
gene id: 224308:BSU_04160
uniprot id: P96574
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Cells lacking this gene cannot grow on either mannitol or glucitol as sole carbon source.
messages: Positively regulates the expression of the mtlAFD operon involved in the uptake and catabolism of mannitol.
messages: The regulatory activity of MtlR is modulated by phosphorylation and dephosphorylation of the various MtlR domains. It becomes activated via phosphoryl group transfer from PEP, EI and HPr on the two conserved histidine residues in the PRD 2 domain, whereas phosphorylation of the EIIA-like domain on His-599 by the PTS EIIB-Mtl domain of MtlA inactivates MtlR (By similarity).
messages: Up-regulated by mannitol or glucitol.

224308:BSU 34770
gene id: 224308:BSU_34770
uniprot id: O06973
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the RapZ-like family.
messages: Cells lacking this gene show a reduction in transformation efficiency and in fraction of cells that express competence.
messages: Displays ATPase and GTPase activities. Can also hydrolyze pNPP. May affect the expression of competence via the phosphorylation of a cellular component.

224308:BSU 03260
gene id: 224308:BSU_03260
uniprot id: P94396
names: Similarity
messages: Belongs to the AB hydrolase superfamily.

224308:BSU 05740
gene id: 224308:BSU_05740
uniprot id: A0A6M3Z893
uniprot id: P19405
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the alkaline phosphatase family.
messages: Binds 1 Mg(2+) ion.
messages: Binds 2 Zn(2+) ions.
messages: Monomer.

224308:BSU 33840
gene id: 224308:BSU_33840
uniprot id: A0A6M3ZG30
uniprot id: O34709
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the GbsR family.
messages: Is not choline-responsive.
messages: Negatively regulates the transcription of the opuC operon. In the absence of GbsR, is also a negative regulator of the opuB operon. Binds to an inverted repeat in the promoter region of the operons.

224308:BSU 02030
gene id: 224308:BSU_02030
uniprot id: O31435
names: Similarity
messages: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

224308:BSU 12260
gene id: 224308:BSU_12260
uniprot id: O34428
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 15320
gene id: 224308:BSU_15320
uniprot id: A0A6M3ZAY0
uniprot id: P06222
names: Function
names: PTM
names: Similarity
messages: Belongs to the sigma-70 factor family.
messages: Proteolytically cleaved in the N-terminus by SpoIIGA to yield the active peptide.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes.

224308:BSU 05070
gene id: 224308:BSU_05070
uniprot id: P96654
names: Similarity
messages: Belongs to the isochorismatase family.

224308:BSU 15080
gene id: 224308:BSU_15080
uniprot id: A0A6M4JMT2
uniprot id: O34687
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL32 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 24370
gene id: 224308:BSU_24370
uniprot id: P49784
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 18260
gene id: 224308:BSU_18260
uniprot id: A0A6M3ZBA1
uniprot id: O34421
names: Similarity
messages: Belongs to the acyl-CoA dehydrogenase family.

224308:BSU 19130
gene id: 224308:BSU_19130
uniprot id: O34636
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00690
gene id: 224308:BSU_00690
uniprot id: A0A6M3Z6F8
uniprot id: P37476
names: Cofactor
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.
messages: Belongs to the AAA ATPase family.
messages: Binds 1 zinc ion per subunit.
messages: Cell membrane
messages: Homohexamer (By similarity). Interacts with FloT at midcell (Probable) (PubMed:23651456, PubMed:26297017). Interacts with FloA at midcell (Probable). Another study shows only minor colocalization with FloA or FloT (PubMed:27362352).
messages: Homohexamer.
messages: In the C-terminal section; belongs to the peptidase M41 family.
messages: In the central section; belongs to the AAA ATPase family.
messages: In vitro partially degrades Spo0E, the phosphatase that acts on Spo0A-P. Recognition requires the last 14 residues of Spo0E (PubMed:19332814). Its stabile accumulation requires FlotA and Flot (PubMed:24222488). May degrade EzrA (Probable).
messages: Induced by osmotic shock (0.8 M NaCl) and by heat shock (52 degrees Celsius).
messages: Membrane raft
messages: Necessary only for stage 0 of sporulation.
messages: Required for expression of the stage 0 sporulation gene Spo0A, as well as for a normal heat or osmotic stress response, cells lacking this gene grow as long filaments and tend to lyse upon entry into stationary phase. Secretion of some extracellular proteins is also reduced (PubMed:9076729). Loss of biofilm formation (PubMed:22882210).

224308:BSU 36450
gene id: 224308:BSU_36450
uniprot id: A0A6M3ZJW8
uniprot id: P94577
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 24390
gene id: 224308:BSU_24390
uniprot id: P49782
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: 4000-fold reduction in sporulation efficiency.
messages: Cell membrane
messages: Interacts with SpoIIIJ and YqjG.
messages: Required during sporulation for activation of sigma factor SpoIIIG/SigG after engulfment is completed in the prespore. Overexpression in the absence of SpoIIIJ is synthetically lethal.

224308:BSU 24980
gene id: 224308:BSU_24980
uniprot id: A0A6M4JLF7
uniprot id: P46339
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the binding-protein-dependent transport system YqgGHIJK. Probably responsible for the translocation of the substrate across the membrane (By similarity).
messages: Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.

224308:BSU 16060
gene id: 224308:BSU_16060
uniprot id: A0A6M3ZAQ6
uniprot id: O31744
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RNase HII family.
messages: Cytoplasm
messages: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
messages: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.

224308:BSU 16980
gene id: 224308:BSU_16980
uniprot id: P45693
names: Function
messages: Induced early in sporulation under the control of sigma-H. Interferes with sporulation at an early stage. Seems to play a positive role in allowing cells to progress beyond stage V of sporulation.

224308:BSU 13240
gene id: 224308:BSU_13240
uniprot id: O34911
names: Function
names: Subunit
messages: Homodimer in vitro. In vivo, may be a part of an ABC transporter complex which is composed of two ATP-binding proteins (YkoD), two transmembrane proteins (YkoC and YkoE) and a solute-binding protein (YkoF) (Probable).
messages: Part of the ABC transporter complex YkoCDEF that could transport hydroxymethylpyrimidine (HMP) and/or thiamine. Could also transport other HMP-containing products. Binds thiamine via its HMP moiety.

224308:BSU 28070
gene id: 224308:BSU_28070
uniprot id: A0A6M3ZES9
uniprot id: P15378
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase A24 family.
messages: By several proteins including Spo0A, Spo0H, Sin, AbrB, DegS, DegU, ComA, ComB and ComK.
messages: Cell membrane
messages: Membrane
messages: Plays a role in type II pseudopili formation by proteolytically removing the leader sequence from substrate proteins and subsequently monomethylating the alpha-amino group of the newly exposed N-terminal phenylalanine. Substrates include proteins required for biogenesis of the type II general secretory apparatus.
messages: Zinc is required for the N-terminal methylation of the mature pilin, but not for signal peptide cleavage.

224308:BSU 14800
gene id: 224308:BSU_14800
uniprot id: O07634
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Forespore inner membrane
messages: Mutant shows a reduced rate of spore germination in L-alanine.
messages: Probably contributes, directly or indirectly, to early events in germination.

224308:BSU 09920
gene id: 224308:BSU_09920
uniprot id: O08455
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Very low induction by mitomycin C and UV irradiation; probably a member of the yhaONM operon.

224308:BSU 38240
gene id: 224308:BSU_38240
uniprot id: A0A6M3ZHN0
uniprot id: P39599
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
messages: Cell membrane
messages: Membrane

224308:BSU 18690
gene id: 224308:BSU_18690
uniprot id: O34983
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 18230
gene id: 224308:BSU_18230
uniprot id: A0A6M4JN62
uniprot id: O34873
names: Function
names: Similarity
names: Subunit
messages: Belongs to the HMG-CoA lyase family.
messages: Homodimer and homotetramer.
messages: Involved in the catabolism of branched amino acids such as leucine.

224308:BSU 35400
gene id: 224308:BSU_35400
uniprot id: A0A6M3ZJP8
uniprot id: P96501
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum
messages: Belongs to the bacterial flagellin family.
messages: Secreted

224308:BSU 06020
gene id: 224308:BSU_06020
uniprot id: A0A6M4JDU6
uniprot id: P28599
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the GroES chaperonin family.
messages: By heat shock.
messages: Cytoplasm
messages: Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.
messages: Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.

224308:BSU 00560
gene id: 224308:BSU_00560
uniprot id: P37554
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Deletion of the gene leads to aberrantly formed spores (PubMed:8755877). Mutant spores release their dipicolinic acid (DPA) via germinant receptor (GR)-dependent germination more rapidly than wild-type spores. Spores are also more sensitive to UV radiation and outgrow slowly (PubMed:22522895).
messages: Expression is regulated by the sporulation transcription factor sigma G.
messages: Homotetramer (PubMed:18996130) (Probable). Two monomers dimerize via their N-terminal swapped-hairpin domains. These dimers further associate into tetramers through helical interactions between their C-terminal GAF-like domains (PubMed:18996130).
messages: The N-terminal domain binds DNA non-specifically and the C-terminal GAF-like domain is crucial to its correct folding and function. The non-specific DNA-binding activity of the N-terminal domain is modulated by the C-terminal domain, which perhaps in combination with another unknown factor, confers activity and specificity.
messages: To B.subtilis AbrB and Abh.
messages: Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).

224308:BSU 39470
gene id: 224308:BSU_39470
uniprot id: P54955
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the peptidase M20 family.
messages: Binds 2 divalent metal cations per subunit.
messages: Cells lacking this gene do not lose the ability to grow on 2SC as the sulfur source because of the presence of other deacetylases that can compensate for scmP (yxeP) deficiency. In a triple mutant lacking this gene, ytnL and yhaA, the levels of NAC highly increases after addition of 2SC.
messages: Probably catalyzes the deacetylation of N-acetylcysteine (NAC) to acetate and cysteine. Is involved in a S-(2-succino)cysteine (2SC) degradation pathway that allows B.subtilis to grow on 2SC as a sole sulfur source, via its metabolization to cysteine.

224308:BSU 12330
gene id: 224308:BSU_12330
uniprot id: A0A6M3ZD69
uniprot id: O35045
names: Cofactor
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 14990
gene id: 224308:BSU_14990
uniprot id: O34412
names: Function
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Regulates sporulation prior to stage II. Positively controls the competence regulator ComK at a post-transcriptional level. May modulate the translation, stability or activity of ComS. May work together with YmcA to regulate community development.

224308:BSU 23420
gene id: 224308:BSU_23420
uniprot id: P40868
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 22550
gene id: 224308:BSU_22550
uniprot id: P46912
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome b family.
messages: Binds 2 heme b groups non-covalently with two histidine residues as axial ligands.
messages: Binds one heme group covalently by a single cysteine link with no axial amino acid ligand.
messages: Cell membrane
messages: Component of the menaquinol:cytochrome c reductase complex.
messages: The main subunits of the menaquinol:cytochrome c complex are a Rieske-type iron-sulfur protein (QcrA), a cytochrome b (QcrB) and a cytochrome c (QcrC).
messages: This cytochrome b is similar to other bacterial and plant chloroplast cytochrome b6 proteins in binding one of three hemes with a single thioether bond.

224308:BSU 38550
gene id: 224308:BSU_38550
uniprot id: A0A6M3ZHM6
uniprot id: P39576
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
messages: Inhibited by canaline.
messages: Transaminates branched-chain amino acids and ketoglutarate.

224308:BSU 10650
gene id: 224308:BSU_10650
uniprot id: O06714
names: Function
names: Similarity
names: Subunit
messages: Belongs to the SMC family. SbcC subfamily.
messages: Heterodimer of SbcC and SbcD.
messages: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity (By similarity).

224308:BSU 39980
gene id: 224308:BSU_39980
uniprot id: P42106
names: Cofactor
names: Function
names: Subunit
messages: Binds 2 Fe(2+) ions per subunit.
messages: Homodimer.
messages: Performs the first step in the degradation of the flavonoid quercetin by a dioxygenase reaction. The enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin yielding the depside 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. This involves the remarkable dioxygenolytic cleavage of two carbon-carbon bonds.

224308:BSU 05930
gene id: 224308:BSU_05930
uniprot id: A0A6M3Z7Y0
uniprot id: O05517
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Acetylates the N-terminal alanine of ribosomal protein S18.
messages: Acetylates the N-terminal alanine of ribosomal protein bS18.
messages: Belongs to the acetyltransferase family. RimI subfamily.
messages: Cytoplasm

224308:BSU 25330
gene id: 224308:BSU_25330
uniprot id: P46344
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PgpH phosphodiesterase family.
messages: Cell membrane
messages: In strain 168 grown in minimal medium and glutamate, 3.5-fold increase in c-di-AMP levels, while a double pgpH-gdpP mutant has 4.2-fold increased levels of in c-di-AMP; double mutants die on solid medium after 2 days (PubMed:26240071).
messages: Probably has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP); may be the major c-di-AMP PDE in the cell (PubMed:26240071). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).

224308:BSU 36010
gene id: 224308:BSU_36010
uniprot id: A0A6M4JPS5
uniprot id: Q04789
names: Cofactor
names: Induction
names: Similarity
messages: Belongs to the TPP enzyme family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.

224308:BSU 09130
gene id: 224308:BSU_09130
uniprot id: A0A6M3Z8T8
uniprot id: P54596
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.
messages: Cell membrane
messages: Mediates uptake of L-cystine, the oxidized form of L-cysteine. Although it is more specific for L-cystine, it could also transport a much broader range of amino acids and sulfur compounds including S-methylcysteine.
messages: Membrane
messages: More strongly expressed in the presence of methionine than in presence of sulfate. Strongly inhibited by seleno-DL-cystine.

224308:BSU 34330
gene id: 224308:BSU_34330
uniprot id: P71054
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance.
messages: Repressed by SinR.

224308:BSU 31070
gene id: 224308:BSU_31070
uniprot id: A0A6M4JMB7
uniprot id: P71015
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the GbsR family.
messages: Intracellular choline sensor. Binding to choline induces conformational changes and relieves the repressing effects of GbsR on expression.
messages: Negatively regulates the expression of the gbsAB and opuB operons. Required to control expression of these genes in response to choline availability. Also required to down-regulate glycine betaine production once cellular adjustment to high osmolarity has been achieved.

224308:BSU 34490
gene id: 224308:BSU_34490
uniprot id: A0A6M3ZG92
uniprot id: O32262
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 38700
gene id: 224308:BSU_38700
uniprot id: A0A6M3ZLN6
uniprot id: P94370
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: Negatively regulated by both YxlD and YxlE.
messages: Positively autoregulated. Induced upon nitrogen starvation.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of the sigY-yxlCDEFG operon upon nitrogen starvation. Also positively regulates ybgB.

224308:BSU 27250
gene id: 224308:BSU_27250
uniprot id: A0A6M3ZEV1
uniprot id: O05394
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the trans-sulfuration enzymes family.
messages: By methionine. Repressed by sulfate and cysteine.
messages: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide.
messages: No visible phenotype.

224308:BSU 03440
gene id: 224308:BSU_03440
uniprot id: A0A6M3Z7W5
uniprot id: P39209
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Membrane

224308:BSU 31630
gene id: 224308:BSU_31630
uniprot id: A0A6M4JMH1
uniprot id: O05229
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 28970
gene id: 224308:BSU_28970
uniprot id: A0A6M3ZFC2
uniprot id: P06568
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TVP38/TMEM64 family.
messages: Cell membrane
messages: Membrane

224308:BSU 40110
gene id: 224308:BSU_40110
uniprot id: A0A6M4JR50
uniprot id: P42973
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyl hydrolase 1 family.
messages: Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.
messages: Expressed during stationary-phase and exponential-phase of growth.
messages: Up-regulated by the aryl-beta-D-glucoside salicin.

224308:BSU 24840
gene id: 224308:BSU_24840
uniprot id: P54496
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Although its sequence similarity with the glycerol phosphate lipoteichoic acid synthase LtaS, it cannot restore staphylococcal growth and LTA synthesis after ltaS depletion.
messages: Belongs to the LTA synthase family.
messages: Cell membrane
messages: Proteolytically cleaved.
messages: Secreted

224308:BSU 16299
gene id: 224308:BSU_16299
uniprot id: C0H412
names: Disruption Phenotype
names: Function
messages: Mutation abolishes swarming motility, reduces swimming speed and torque of tethered flagella. It does not affect flagellar assembly, flagellar number or surfactant production.
messages: Required for swarming motility. Increases flagellar power, probably via the flagellar stator components MotA and MotB.

224308:BSU 01770
gene id: 224308:BSU_01770
uniprot id: A0A6M3ZC24
uniprot id: O34824
names: Cofactor
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: Activated by phosphorylation.
messages: Belongs to the phosphohexose mutase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate (By similarity). Glucosamine-1-phosphate is used for cell wall biosynthesis (Probable).
messages: Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.
messages: Constitutively expressed, part of the cdaA-cdaR-glmM-glmS operon (PubMed:23192352).
messages: Homodimer, may form a complex with CdaA.

224308:BSU 09310
gene id: 224308:BSU_09310
uniprot id: A0A6M4JGD0
uniprot id: P18159
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the phosphohexose mutase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the interconversion between glucose-6-phosphate and alpha-glucose-1-phosphate. This is the first step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA). Has a role in the biosynthesis of all phosphate-containing envelope polymers, since glucose-1-phosphate is the precursor of UDP-glucose, which serves as a glucosyl donor not only for the biosynthesis of LTA but also for wall teichoic acids (WTAs). Is required for biofilm formation. This is likely due to another role of UDP-glucose, which might also act as a metabolic signal regulating biofilm formation or may be involved in some unknown biosynthetic pathway essential for biofilm formation, e.g. the synthesis of an exopolysaccharide.

224308:BSU 33310
gene id: 224308:BSU_33310
uniprot id: P49936
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane raft
messages: Part of the ABC transporter complex FhuBGCD involved in iron(3+)-hydroxamate import. Responsible for the translocation of the substrate across the membrane (By similarity).
messages: The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB and FhuG) and a solute-binding protein (FhuD or YxeB).

224308:BSU 19579
gene id: 224308:BSU_19579
uniprot id: C0H431
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 07080
gene id: 224308:BSU_07080
uniprot id: A0A6M3Z8Q4
uniprot id: O31529
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the glycosyl hydrolase 42 family.
messages: Homotrimer.
messages: May play a role in the degradation of rhamnogalacturonan derived from plant cell walls.
messages: No chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) hydrolyzation.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 25510
gene id: 224308:BSU_25510
uniprot id: A0A6M4JKY0
uniprot id: P37949
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.
messages: Cell membrane
messages: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.

224308:BSU 04920
gene id: 224308:BSU_04920
uniprot id: P96640
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10690
gene id: 224308:BSU_10690
uniprot id: O06718
names: Developmental Stage
names: Function
names: Induction
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 11210
gene id: 224308:BSU_11210
uniprot id: A0A6M3ZEN5
uniprot id: P68729
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the acetylglutamate kinase family. ArgB subfamily.
messages: Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.
messages: Cytoplasm

224308:BSU 23120
gene id: 224308:BSU_23120
uniprot id: A0A6M4JIR9
uniprot id: P35163
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Interacts with the RNA polymerase core.
messages: Member of the two-component regulatory system ResD/ResE. Required for the expression of resA, ctaA, qcrABC and fnr; activation role in global regulation of aerobic and anaerobic respiration.
messages: Phosphorylated by ResE.

224308:BSU 15580
gene id: 224308:BSU_15580
uniprot id: A0A6M4JJU8
uniprot id: O34734
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
messages: Cell membrane
messages: Involved in the import of sulfate.
messages: Membrane
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 24270
gene id: 224308:BSU_24270
uniprot id: A0A6M4JKL7
uniprot id: P54523
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the transketolase family. DXPS subfamily.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).
messages: Homodimer.

224308:BSU 08310
gene id: 224308:BSU_08310
uniprot id: P94440
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Induced in response to linearmycins and other polyenes via the two-component regulatory system LnrJ/LnrK.
messages: Required for resistance to linearmycins, a family of antibiotic-specialized metabolites produced by some streptomycetes (PubMed:26647299, PubMed:28461449). Part of the ABC transporter complex LnrLMN that probably facilitates linearmycin removal from the membrane. Responsible for energy coupling to the transport system (PubMed:28461449). Also mediates KinC-dependent biofilm morphology (PubMed:28461449).
messages: The complex is composed of two ATP-binding proteins (LnrL) and two transmembrane proteins (LnrM and LnrN).

224308:BSU 33110
gene id: 224308:BSU_33110
uniprot id: O32200
names: Disruption Phenotype
names: Induction
messages: Induced, via the two-component regulatory system LiaS/LiaR, by antibiotics (vancomycin, bacitracin, nisin and ramoplanin), cationic antimicrobial peptides (human LL-37 and porcine PG-1), Triton X-100 and severe secretion stress.
messages: Up-regulates expression of liaI which in turn induces the liaRS two-component regulatory system.

224308:BSU 24750
gene id: 224308:BSU_24750
uniprot id: A0A6M4JKX8
uniprot id: P54505
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0053 family.
messages: Cell membrane
messages: Membrane

224308:BSU 03690
gene id: 224308:BSU_03690
uniprot id: O31479
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 36500
gene id: 224308:BSU_36500
uniprot id: P94572
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 21750
gene id: 224308:BSU_21750
uniprot id: A0A6M3ZD20
uniprot id: P54178
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SCO1/2 family.
messages: Cell membrane
messages: Monomer.
messages: Necessary for insertion of copper into the active site of cytochrome c oxidase. May play a role in copper homeostasis or redox signaling.

224308:BSU 15760
gene id: 224308:BSU_15760
uniprot id: O34779
names: Cofactor
names: Function
messages: Binds 2 manganese ions per subunit.
messages: Protein phosphatase that dephosphorylates PrkC and EF-G (elongation factor G, fusA). prpC and prkC are cotranscribed, which suggests that they form a functional couple in vivo, PrpC's primary role being possibly to counter the action of PrkC. May be involved in sporulation and biofilm formation. Does not seem to be involved in stress response. Dephosphorylates phosphorylated CgsA, EF-Tu and YezB (PubMed:19246764).

224308:BSU 11750
gene id: 224308:BSU_11750
uniprot id: Q08311
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Disulfide cross-linked either to itself or to CotZ.
messages: Spore coat
messages: To B.subtilis CotZ.

224308:BSU 37350
gene id: 224308:BSU_37350
uniprot id: O07623
names: Caution
names: Developmental Stage
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacteriocin class V family.
messages: Has bacteriocidal activity against some Gram-positive bacteria such as Listeria, some species of Bacillus and E.faecium (PubMed:10572140, PubMed:19633086, PubMed:3936839). A single mutation (Thr-14-Ile) confers hemolytic activity against rabbit and human blood (PubMed:19633086).
messages: PubMed:3936839 sequence does not report residues in positions 30 and 39 probably due to their modification, and reports a cyclic permutation of the peptide sequence.
messages: Secreted
messages: The production of subtilosin A begins at the end of vegetative growth and finishes before spore formation.
messages: This sactipeptide undergoes unique processing steps that include proteolytic cleavage after Glu-8, and covalent linkage of the alpha-amino of Asn-9 with the carboxyl of Gly-43 to form a cyclopeptide (PubMed:12696888, PubMed:22366720). Thioether cross-links are formed between cysteines and the alpha-carbons of other amino acids, Cys-12 to Phe-39, Cys-15 to Thr-36, and Cys-21 to Phe-30 (PubMed:12696888, PubMed:22366720). In forming these cross-links, Thr-36 and Phe-39 are converted to D-amino acids (PubMed:12696888). Propeptide cleavage and cyclopeptide formation only occur after all 3 thioether cross-links are formed (PubMed:22366720).
messages: Transcription is highly induced by oxygen limitation and is under dual and independent control of Spo0A-AbrB and ResDE.

224308:BSU 27100
gene id: 224308:BSU_27100
uniprot id: A0A6M3ZES3
uniprot id: O05406
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rht family.
messages: Cell membrane

224308:BSU 01935
gene id: 224308:BSU_01935
uniprot id: O31425
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Membrane
messages: Required for production of the bacteriocin SkfA.
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 22170
gene id: 224308:BSU_22170
uniprot id: P50840
names: Similarity
names: Subunit
messages: Belongs to the methyltransferase superfamily.
messages: Interacts with the RNA polymerase core.

224308:BSU 04470
gene id: 224308:BSU_04470
uniprot id: A0A6M3Z7T9
uniprot id: P96603
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.
messages: Cell membrane
messages: Induced by succinate and fumarate. Expression of dctA requires the DctB, DctS and DctR proteins and is decreased in the presence of malate.
messages: Membrane
messages: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate across the membrane.
messages: Responsible for the transport of succinate and fumarate, but not malate, across the membrane.

224308:BSU 00680
gene id: 224308:BSU_00680
uniprot id: A0A6M3Z6H8
uniprot id: P37472
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family.
messages: Cytoplasm
messages: Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to the N9 position of the 6-oxopurines hypoxanthine and guanine to form the corresponding ribonucleotides IMP (inosine 5'-monophosphate) and GMP (guanosine 5'-monophosphate), with the release of PPi.

224308:BSU 29500
gene id: 224308:BSU_29500
uniprot id: O34806
names: Developmental Stage
names: Induction
names: Subcellular Location Annotation
messages: Could be expressed in the forespore during sporulation.
messages: Expression is probably sigma F-dependent.
messages: Spore core

224308:BSU 27970
gene id: 224308:BSU_27970
uniprot id: P26937
names: Cofactor
names: Developmental Stage
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase M50B family.
messages: Binds 1 zinc ion per subunit.
messages: Could be held inactive when BofA provides a fourth zinc ligand, preventing access to a water molecule and the sequence of pro sigma-K.
messages: Forespore outer membrane
messages: Forms a complex with SpoIVFA and BofA localized in the mother-cell membrane surrounding the forespore.
messages: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation. Processes the pro-sigma K factor.
messages: Transcribed during the stage II, but not required until stage IV of sporulation.

224308:BSU 04930
gene id: 224308:BSU_04930
uniprot id: P96641
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39290
gene id: 224308:BSU_39290
uniprot id: P40737
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YxiD (PubMed:34280190). Neutralizes the toxic activity of cognate toxin YxiD upon expression in E.coli. Does not have immunity protein activity on other LXG toxins (PubMed:22200572).
messages: Probably interacts with cognate toxin YxiD but not with other non-cognate toxins. The interaction inhibits the toxic activity of YxiD (Probable).

224308:BSU 24720
gene id: 224308:BSU_24720
uniprot id: P25954
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GSP F family.
messages: Cell membrane
messages: Required for transformation and DNA binding.

224308:BSU 40500
gene id: 224308:BSU_40500
uniprot id: A0A6M3ZI30
uniprot id: P37437
names: Function
names: Similarity
messages: Belongs to the bacterial ribosomal protein bL9 family.
messages: Binds to the 23S rRNA.

224308:BSU 00430
gene id: 224308:BSU_00430
uniprot id: P37548
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: According to PubMed:10714992, cells have an altered coat protein composition; CotT, YeeK, YxeE, CotF, SafA and SpoIVA proteins are present at increased levels and/or exist as precursors. According to PubMed:16751597, cells show no effects on vegetative growth or spore resistance to heat, chloroform or lysozyme. The germination of YabG mutant spores in L-alanine and in a mixture of L-asparagine, D-glucose, D-fructose and potassium chloride was the same as that of the wild-type spores. Heat treatment for 20 minutes at 60 degrees Celsius, which maximally activates the Tgl enzymatic activity, causes cross-linking of GerQ in isolated YabG-deleted spores but not in Tgl/YabG double-mutant spores. Additionally, the germination frequency of the Tgl/YabG double-mutant spores in the presence of L-alanine with or without heat activation at 60 degrees Celsius is lower than that of wild-type spores.
messages: Belongs to the peptidase U57 family.
messages: Cleaves the spore coat proteins SpoIVA and SafA. May cooperate with tgl to mediate the temperature-dependent cross-linking of coat proteins like GerQ.
messages: Expressed in the mother cell compartment from T4 of sporulation.
messages: Forespore outer membrane
messages: Induced by SigK.

224308:BSU 08540
gene id: 224308:BSU_08540
uniprot id: A0A6M4JG53
uniprot id: O31577
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 25340
gene id: 224308:BSU_25340
uniprot id: P46343
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PhoH family.
messages: Cytoplasm

224308:BSU 37500
gene id: 224308:BSU_37500
uniprot id: A0A6M4JQC0
uniprot id: P70998
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis has only one biosynthetic pathway from agmatine to spermidine to produce polyamines.
messages: Belongs to the spermidine/spermine synthase family.
messages: Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.
messages: Cytoplasm
messages: Homodimer or homotetramer.
messages: Involved in the cell growth and proliferation. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine (Probable).

224308:BSU 38210
gene id: 224308:BSU_38210
uniprot id: A0A6M3ZIW1
uniprot id: P39602
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GtrA family.
messages: Cell membrane
messages: Membrane

224308:BSU 25170
gene id: 224308:BSU_25170
uniprot id: A0A6M3ZE75
uniprot id: P54472
names: Similarity
names: Subunit
messages: Belongs to the GTP cyclohydrolase I type 2/NIF3 family.
messages: Homohexamer.

224308:BSU 15870
gene id: 224308:BSU_15870
uniprot id: A0A6M3ZAK9
uniprot id: O34942
names: Function
names: Similarity
messages: Belongs to the helicase family. RecG subfamily.
messages: Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA) (By similarity).
messages: Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA).

224308:BSU 36070
gene id: 224308:BSU_36070
uniprot id: P39801
names: Function
names: Subunit
messages: Homodimer.
messages: May be a morphogenetic protein that is required for the incorporation of protein CotB into the spore coat.

224308:BSU 08390
gene id: 224308:BSU_08390
uniprot id: A0A6M4JJQ4
uniprot id: O31562
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the metal hydrolase YfiT family.
messages: Binds 1 zinc ion per subunit.
messages: Binds 1 zinc ion per subunit. Is able to bind nickel instead of zinc.
messages: Cytoplasm
messages: Homodimer.
messages: Possible metal-dependent hydrolase.

224308:BSU 19210
gene id: 224308:BSU_19210
uniprot id: O34669
names: Induction
names: Subcellular Location Annotation
messages: Positively regulated by WalR. Expressed primarily during exponential growth, with levels decreasing rapidly at the beginning of the stationary phase.
messages: cell wall

224308:BSU 08060
gene id: 224308:BSU_08060
uniprot id: O31404
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme (By similarity).
messages: Cells lacking this gene are unable to utilize acetoin as carbon source.
messages: Strongly induced by acetoin. Transcriptionally up-regulated by AcoR and sigma-L factor. Subject to catabolite repression by glucose, in a CcpA-independent manner.
messages: Tetramer of 2 alpha and 2 beta subunits.

224308:BSU 00160
gene id: 224308:BSU_00160
uniprot id: P37531
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
messages: Expressed in the mother cell compartment from T2 of sporulation.
messages: Expression is regulated by the sporulation transcription factor sigma E.
messages: Inactivation of the gene does not impair vegetative growth nor prevent the development of resistance to heat, chloroform and lysozyme (PubMed:10419957). According to Kodama et al, the germination of the mutant spores induced by L-alanine is defective. However Chirakkal et al reported that the mutant shows wild-type germination kinetics in L-alanine (PubMed:12177332, PubMed:10419957).
messages: N-acetylglucosaminidase involved in cortex peptidoglycan degradation during germination. Cleaves only partially degraded spore peptidoglycans. Recognizes muramic acid delta-lactam residues specific to spore peptidoglycans.
messages: Spore coat
messages: Was originally thought to have epimerase activity.

224308:BSU 30770
gene id: 224308:BSU_30770
uniprot id: A0A6M3ZFC3
uniprot id: O34385
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 9 family.
messages: Cell membrane
messages: Membrane raft
messages: Null mutant is very sensitive to manganese.
messages: Probably part of ATP-binding cassette (ABC) transport system MntABCD involved in manganese import (Probable). Binds manganese and delivers it to the membrane permease for translocation into the cytoplasm (Probable).
messages: Repressed by MntR in the presence of manganese.
messages: The complex is probably composed of two ATP-binding proteins (MntB), two transmembrane proteins (MntC and MntD) and a solute-binding protein (MntA) (Probable). Interacts with FloT (PubMed:23651456).

224308:BSU 37730
gene id: 224308:BSU_37730
uniprot id: P39639
names: Cofactor
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Binds 2 Fe(2+) or Co(2+) ions per subunit.
messages: Cytoplasm
messages: Monomer.
messages: Part of the bacABCDEF operon responsible for the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:15609023, PubMed:20052993). BacB catalyzes the allylic isomerization of the endocyclic-delta(4),delta(8)-7R-dihydro-hydroxyphenylpyruvate (en-H2HPP) to generate a mixture of 3E,7R- and 3Z, 7R-olefins (E/Z ration of 3/1) of the exocyclic-delta(3),delta(5)-dihydro-hydroxyphenylpyruvate (ex-H2HPP) (PubMed:20052993, PubMed:22483065, PubMed:22765234, PubMed:19776011).
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDEF operon and BacG, and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 11610
gene id: 224308:BSU_11610
uniprot id: A0A6M4JFA3
uniprot id: O31612
names: Activity Regulation
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allosterically inhibited by NADP and activated by quinolinic acid. Strongly inhibited by HgCl(2).
messages: Belongs to the NAD kinase family.
messages: Cytoplasm
messages: Divalent metal ions. Both Ca(2+) and Mn(2+) ions are more effective activators than zinc, cobalt, copper and magnesium ions at low concentrations.
messages: Homodimer.
messages: Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.
messages: Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates (GTP, UTP) as well as inorganic polyphosphate (poly(P)) as a source of phosphorus.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 08110
gene id: 224308:BSU_08110
uniprot id: A0A6M4JG23
uniprot id: O31552
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspH family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination. Expression is sigma G-dependent.
messages: Spore core

224308:BSU 24190
gene id: 224308:BSU_24190
uniprot id: P54525
names: Similarity
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

224308:BSU 10790
gene id: 224308:BSU_10790
uniprot id: A0A6M4JIH4
uniprot id: O05272
names: Function
names: Similarity
messages: Asparagine synthetase involved in sporulation.
messages: Belongs to the asparagine synthetase family.

224308:BSU 14590
gene id: 224308:BSU_14590
uniprot id: P21882
names: Function
names: Subunit
messages: Heterodimer of an alpha and a beta chain.
messages: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity.
messages: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 27140
gene id: 224308:BSU_27140
uniprot id: A0A6M3ZEU3
uniprot id: O05402
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the acyltransferase 3 family.
messages: Cell membrane

224308:BSU 18970
gene id: 224308:BSU_18970
uniprot id: O34784
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31322
gene id: 224308:BSU_31322
uniprot id: Q795M8
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05470
gene id: 224308:BSU_05470
uniprot id: A0A6M4JDF7
uniprot id: C0SP78
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.
messages: Cell membrane
messages: Deletion mutant is manganese sensitive and accumulates high levels of intracellular manganese. Effects are exacerbated in a mneP-mneS double mutant.
messages: Membrane
messages: Primary efflux pump for manganese. May prevent manganese intoxication.
messages: Transcriptionally activated by MntR in response to manganese excess. Expressed at a basal level in the absence of MntR.

224308:BSU 15240
gene id: 224308:BSU_15240
uniprot id: A0A6M4JIE2
uniprot id: P16655
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsQ/DivIB family. DivIB subfamily.
messages: Cell division protein that may be involved in stabilizing or promoting the assembly of the division complex.
messages: Cell division protein that may be involved in stabilizing or promoting the assembly of the division complex. Plays an essential role in division at high temperatures, maybe by protecting FtsL from degradation or by promoting formation of the FtsL-DivIC complex. May modulate the transpeptidase activity of PBP-2B. Also required for efficient sporulation at all temperatures. Could be directly involved in the engulfment process or be required to form a sporulation septum competent for engulfment. Influences the Spo0J/Soj system of chromosome segregation.
messages: Cell membrane
messages: Interacts with FtsL, DivIC and PBP-2B.
messages: Mutants show a drastic decrease in sporulation efficiency. Polar septation is delayed and less efficient, and the completed septa are thicker than those of the wild type. Mutants are also unable to undergo engulfment.
messages: The extracellular region contains three distinct subdomains: a membrane proximal alpha domain, a central beta domain and an unstructured C-terminal gamma domain. The C-terminal region of the beta domain is critical for interaction with PBP-2B. Contains multiple septal localization epitopes that are located within the transmembrane region and within the extracellular region. These epitopes may represent sites of interaction with other divisomal proteins.

224308:BSU 37690
gene id: 224308:BSU_37690
uniprot id: A0A6M3ZK57
uniprot id: P39643
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
messages: Cytoplasm
messages: Homodimer.
messages: Part of the bacABCDEF operon responsible for the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:20052993). Catalyzes the reductive amination of the C2 ketone of tetrahydro-hydroxyphenylpyruvate (H4HPP), with L-Phe as an amino donor, to yield tetrahydrotyrosine (H4Tyr) diastereomer (PubMed:22765234). D-Phe is not an effective amino donor (PubMed:22765234). BacF associated to BacG converts 3E,7R- and 3Z,7R-ex-H2HPP to 2S,4R,7R- and 2S,4S,7R-H4Tyr, respectively. Given that bacilysin has the 2S,4S stereochemistry in its anticapsin moiety, it is likely that the 2S,4S-H4Tyr is the diastereomer used for the biosynthesis (PubMed:22765234).
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDEF operon and BacG, and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 35580
gene id: 224308:BSU_35580
uniprot id: A0A6M4JPR2
uniprot id: O32271
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Activated by phosphorylation; inhibited by dephosphorylation.
messages: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid.
messages: Cytoplasm
messages: Phosphorylated by YwqD and dephosphorylated by YwqE in vitro.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 03910
gene id: 224308:BSU_03910
uniprot id: A0A6M3Z7P5
uniprot id: P94428
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family.
messages: Catalyzes the NADP(+) dependent oxidation of succinate semialdehyde to succinate.
messages: Cells lacking this gene are unable to use GABA as sole nitrogen source (PubMed:12123465). No effect on vanillin degradation (PubMed:26658822).
messages: Expressed from the major gabT promoter and from an additional gabD promoter.
messages: Transcriptionally regulated by GabR in the presence of GABA. Up-regulated under low pH conditions and ethanol. Expression from the gabD promoter was induced by growth in the presence of glutamate.

224308:BSU 00070
gene id: 224308:BSU_00070
uniprot id: A0A6M4JJ14
uniprot id: P05653
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
messages: Belongs to the topoisomerase GyrA/ParC subunit family.
messages: Belongs to the type II topoisomerase GyrA/ParC subunit family.
messages: Cytoplasm
messages: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
messages: Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

224308:BSU 37850
gene id: 224308:BSU_37850
uniprot id: P39627
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To M.jannaschii MJ1062.

224308:BSU 37630
gene id: 224308:BSU_37630
uniprot id: A0A6M4JQD9
uniprot id: P39649
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 06470
gene id: 224308:BSU_06470
uniprot id: A0A6M3Z8Q7
uniprot id: P12041
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
messages: Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

224308:BSU 35450
gene id: 224308:BSU_35450
uniprot id: A0A6M3ZH18
uniprot id: P39147
names: Function
names: Similarity
messages: Belongs to the ComF/GntX family.
messages: Involved in transformation (competence for DNA uptake).

224308:BSU 39460
gene id: 224308:BSU_39460
uniprot id: A0A6M3ZI44
uniprot id: P54956
names: Similarity
messages: Belongs to the PrpD family.

224308:BSU 25610
gene id: 224308:BSU_25610
uniprot id: P54458
names: Function
names: Similarity
messages: Belongs to the methyltransferase superfamily.
messages: May be a S-adenosyl-L-methionine (SAM)-dependent methyltransferase.

224308:BSU 01760
gene id: 224308:BSU_01760
uniprot id: O34659
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Constitutively expressed, part of the cdaA-cdaR-glmM-glmS operon (PubMed:23192352).
messages: Interacts with CdaA (PubMed:23192352, PubMed:26240071).
messages: No visible phenotype.
messages: Upon coexpression in E.coli stimulates the diadenylate cyclase activity of CdaA about 20-fold (PubMed:23192352). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).

224308:BSU 01960
gene id: 224308:BSU_01960
uniprot id: O31428
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: Accelerated cannibalism by skf- cells is seen on solid media but not in liquid media.
messages: By Spo0A (PubMed:12817086) and PhoP, during nutrient starvation, especially phosphate starvation. Repressed by AbrB during normal growth when nutrients are plentiful, in association with the transcriptional repressor Abh.
messages: Cell membrane
messages: Probably part of the ABC transporter SkfEF involved in the export of the bacteriocin SKF. Probably responsible for the translocation of bacteriocin SkfA across the membrane.
messages: When the skfA-skfB-skfC-skfE-skfF-skfG-skfH operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086).

224308:BSU 25569
gene id: 224308:BSU_25569
uniprot id: C0H453
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 35160
gene id: 224308:BSU_35160
uniprot id: A0A6M3ZGE7
uniprot id: O34863
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. UvrA family.
messages: Cytoplasm
messages: Forms a heterotetramer with UvrB during the search for lesions.
messages: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.

224308:BSU 32200
gene id: 224308:BSU_32200
uniprot id: O32117
names: Cofactor
names: Similarity
messages: Belongs to the NADH dehydrogenase family.
messages: Binds 1 FAD per subunit.

224308:BSU 39260
gene id: 224308:BSU_39260
uniprot id: A0A6M3ZHY9
uniprot id: P40740
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyl hydrolase 1 family.
messages: Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.
messages: Highly up-regulated by aryl-beta-D-glucosides such as salicin or 4-methylumbelliferyl-beta-D-glucopyranoside (MUG).
messages: Significantly expressed throughout all stages of growth or development.

224308:BSU 01490
gene id: 224308:BSU_01490
uniprot id: A0A6M4JCJ7
uniprot id: P70974
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL13 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Binds to Obg (AC P20964) (PubMed:10781545).
messages: Part of the 50S ribosomal subunit.
messages: This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.

224308:BSU 30750
gene id: 224308:BSU_30750
uniprot id: A0A6M4JMN7
uniprot id: O35024
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-3 integral membrane protein family.
messages: Cell membrane
messages: Membrane
messages: Probably part of the ABC transporter complex MntABCD involved in manganese import. Probably responsible for the translocation of the substrate across the membrane.
messages: Repressed by MntR in the presence of manganese.
messages: The complex is probably composed of two ATP-binding proteins (MntB), two transmembrane proteins (MntC and MntD) and a solute-binding protein (MntA).

224308:BSU 24130
gene id: 224308:BSU_24130
uniprot id: A0A6M3ZDM0
uniprot id: P45859
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the PrpD family.
messages: Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.
messages: Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Has both 2-methylcitrate dehydratase and citrate dehydratase activities. Catalyzes the dehydration of 2-methylcitrate (2-MC) to yield 2-methyl-cis-aconitate, and the dehydration of citrate to yield cis-aconitate. Cannot form isocitrate. Uses either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).
messages: Subject to catabolite repression.

224308:BSU 11600
gene id: 224308:BSU_11600
uniprot id: O31611
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Allosterically regulated by its own products; pppGpp simulates synthesis 10-fold more than ppGpp. 2 pppGpp molecules bind in a regulatory cleft in the middle of the tetramer in an asymmetric manner. There is a specific contact of Lys-25 to the gamma-phosphate of pppGpp, explaining why pppGpp stimulates activity but ppGpp does not (PubMed:26460002).
messages: Belongs to the RelA/SpoT family.
messages: Expressed during exponential growth through the transition to the stationary phase, but not during entry of cells into stationary phase (PubMed:18067544). Protein continuously expressed with a peak at 2 hours after inoculation, decreasing until 3.5 hours as cells enter stationary phase (at protein level) (PubMed:22950019).
messages: Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). The enzyme binds ATP, then GDP or GTP and catalysis is highly cooperative (PubMed:26460002). In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Probably has a minor role in the stringent response (PubMed:18067544).
messages: Homotetramer (PubMed:26460002).
messages: No visible phenotype, double yjbM-ywaC and triple relA-yjbM-ywaC mutants are also viable (PubMed:18067544).
messages: The synthase activity of YjbM is greater than that of YwaC (PubMed:18067544).

224308:BSU 23450
gene id: 224308:BSU_23450
uniprot id: A0A6M3ZDB4
uniprot id: P07860
names: Activity Regulation
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Association with RNAP core increases during sporulation but not tested stresses (at protein level).
messages: Belongs to the sigma-70 factor family.
messages: In spo-63 (loss of residues 27-255); no spores develop, no induction of sporulation-associated enzymes.
messages: Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma-F activity is released in the prespore (PubMed:8358793). Fin, a second, forespore-specific anti-sigma factor is induced in 2 successive waves by sigma-F and sigma-G, by antagonizing sigma-F it allows the switch to sigma-G factor and progression to the late sporulation development stages (PubMed:21037003).
messages: Interacts transiently with the RNAP core.
messages: Produced in the predivisional sporangium, but does not become active in directing gene expression until after septum formation when its activity is restricted to the forespore (at protein level).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes (PubMed:8759874). Interaction with SpoIIAB inhibits sigma-F activity throughout the cell before the formation of the asymmetric septum; after septation the interaction is confined to the mother cell, and sigma F activity is released in the prespore. Responsible for expression of csfB (the anti-sigma-G factor Gin) (PubMed:8759874). Associates with the RNAP core only in stationary phase cells (PubMed:21710567).

224308:BSU 21690
gene id: 224308:BSU_21690
uniprot id: A0A6M4JNI1
uniprot id: P54154
names: Function
names: Similarity
messages: Belongs to the MsrA Met sulfoxide reductase family.
messages: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
messages: Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. May deal with oxidative damage to alpha/beta-type SASP in spores.

224308:BSU 22410
gene id: 224308:BSU_22410
uniprot id: A0A6M4JKA2
uniprot id: P52999
names: Cofactor
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PanD family.
messages: Binds 1 pyruvoyl group covalently per subunit.
messages: Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.
messages: Cytoplasm
messages: Heterooctamer of four alpha and four beta subunits.
messages: Is synthesized initially as an inactive proenzyme, which is activated by self-cleavage at a specific serine bond to produce a beta-subunit with a hydroxyl group at its C-terminus and an alpha-subunit with a pyruvoyl group at its N-terminus.

224308:BSU 00260
gene id: 224308:BSU_00260
uniprot id: A0A6M4JC57
uniprot id: P37535
names: Similarity
messages: Belongs to the TelA family.

224308:BSU 24630
gene id: 224308:BSU_24630
uniprot id: P54506
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.
messages: Belongs to the peptidase S26B family.
messages: Cell membrane
messages: Mutation impairs colony surface architecture.
messages: Part of the tapA-sipW-tasA operon (PubMed:10464223). Expression is directly repressed by the DNA-binding protein master regulator of biofilm formation SinR and activated by the extracellular matrix regulatory protein RemA (PubMed:16430695, PubMed:23646920). Expressed constitutively at a low level (PubMed:9694797). Also positively regulated by the sporulation transcription factors sigma H and Spo0A and repressed by the transition phase regulatory protein AbrB, probably indirectly (PubMed:10464223).
messages: Required for the cleavage of the signal sequence of TasA and TapA, which are involved in biofilm formation.

224308:BSU 28980
gene id: 224308:BSU_28980
uniprot id: P06567
names: Function
messages: Probably involved in DNA replication.

224308:BSU 25910
gene id: 224308:BSU_25910
uniprot id: A0A6M3ZE70
uniprot id: P24811
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacteriophage holin family. Cp-1 holin subfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 18010
gene id: 224308:BSU_18010
uniprot id: O31820
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioredoxin family.
messages: Cell membrane
messages: No sporulation-related phenotype.

224308:BSU 12580
gene id: 224308:BSU_12580
uniprot id: P39786
names: Function
names: Similarity
names: Subunit
messages: Dimer of a small and a large subunit.
messages: Functions as a terminase.
messages: To B.subtilis YqaT and phage SPP1 terminase large subunit.

224308:BSU 29740
gene id: 224308:BSU_29740
uniprot id: A0A6M4JKW9
uniprot id: P25144
names: Function
names: Subunit
messages: Global transcriptional regulator of carbon catabolite repression (CCR) and carbon catabolite activation (CCA), which ensures optimal energy usage under diverse conditions.
messages: Global transcriptional regulator of carbon catabolite repression (CCR) and carbon catabolite activation (CCA), which ensures optimal energy usage under diverse conditions. Interacts with either P-Ser-HPr or P-Ser-Crh, leading to the formation of a complex that binds to DNA at the catabolite-response elements (cre). Binding to DNA allows activation or repression of many different genes and operons.
messages: Homodimer. Interacts with P-Ser-HPr and P-Ser-Crh.

224308:BSU 31310
gene id: 224308:BSU_31310
uniprot id: O05248
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 12350
gene id: 224308:BSU_12350
uniprot id: A0A6M3ZEZ2
uniprot id: O34896
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Induced by galacturonate, repressed by glucose.
messages: Member of the exu locus which is required for galacturonate utilization.

224308:BSU 22430
gene id: 224308:BSU_22430
uniprot id: A3F319
uniprot id: P52996
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PanB family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
messages: Cytoplasm
messages: Homodecamer; pentamer of dimers.

224308:BSU 10720
gene id: 224308:BSU_10720
uniprot id: O06721
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the GerPA/GerPF family.
messages: Expressed during sporulation, around the time of spore coat synthesis and assembly, in mother cell compartment.
messages: Expression is sigma K-dependent and negatively regulated by GerE.
messages: Required for the formation of functionally normal spores. Could be involved in the establishment of normal spore coat structure and/or permeability, which allows the access of germinants to their receptor.

224308:BSU 25350
gene id: 224308:BSU_25350
uniprot id: P54469
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To B.megaterium SpoIV.

224308:BSU 15280
gene id: 224308:BSU_15280
uniprot id: A0A6M3ZAV0
uniprot id: P28264
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the FtsA/MreB family.
messages: Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.
messages: Cell division protein that is required for the assembly of the Z ring (PubMed:16159787). May serve as a membrane anchor for the Z ring (By similarity). Binds and hydrolyzes ATP (PubMed:11298280). Also involved in sporulation (Probable).
messages: Cell membrane
messages: Homodimer (PubMed:11298280). Interacts with FtsZ (PubMed:16159787).
messages: In B.subtilis, unlike the situation in E.coli, the role of FtsA in the recruitment of other division proteins to the division site appears to be an indirect one, through ensuring that FtsZ forms a proper ring.
messages: Self-interacts. Interacts with FtsZ.
messages: Transcription is controlled by three promoters. Two of these promoters, P1 and P3 are expressed mainly during vegetative growth. The third one, P2, is up-regulated around the onset of sporulation.

224308:BSU 28860
gene id: 224308:BSU_28860
uniprot id: A0A6M4JK86
uniprot id: P55874
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL35 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 24500
gene id: 224308:BSU_24500
uniprot id: P54514
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 39660
gene id: 224308:BSU_39660
uniprot id: P42421
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Phosphorylated by YxdK.
messages: Probable member of the two-component regulatory system YxdK/YxdJ. Positively regulates the expression of the yxdLMyxeA operon by direct interaction with its promoter region. Could also indirectly regulate the expression of the dlt operon.

224308:BSU 31650
gene id: 224308:BSU_31650
uniprot id: A0A6M4JLI4
uniprot id: O05228
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Involved in cholate and Na(+) efflux activities, which may be mechanistically coupled. Does not require other Mrp proteins for its own function.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 30940
gene id: 224308:BSU_30940
uniprot id: A0A6M4JMW5
uniprot id: P39123
names: Function
names: Induction
names: Similarity
messages: Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
messages: Belongs to the glycogen phosphorylase family.
messages: Expressed exclusively on media containing carbon sources that allow efficient sporulation.
messages: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

224308:BSU 16150
gene id: 224308:BSU_16150
uniprot id: A0A6M3ZAR8
uniprot id: P39070
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A double ring-shaped homohexamer of ClpQ is capped on each side by a ring-shaped ClpY homohexamer. The assembly of the ClpQ/ClpY complex is dependent on binding of ATP (By similarity).
messages: A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.
messages: Allosterically activated by HslU binding.
messages: Belongs to the peptidase T1B family. HslV subfamily.
messages: Cytoplasm
messages: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.
messages: Protease subunit of a proteasome-like degradation complex.

224308:BSU 38950
gene id: 224308:BSU_38950
uniprot id: P42319
names: Similarity
messages: To B.subtilis YxjG.

224308:BSU 15980
gene id: 224308:BSU_15980
uniprot id: A0A6M3ZAQ7
uniprot id: P37105
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the GTP-binding SRP family. SRP54 subfamily.
messages: Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.
messages: Cytoplasm
messages: Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
messages: Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components (Probable).
messages: Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY (By similarity). Interacts with a small cytoplasmic RNA (sc-RNA).
messages: Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.

224308:BSU 36360
gene id: 224308:BSU_36360
uniprot id: A0A6M3ZHF0
uniprot id: P94585
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MscL family.
messages: Cell membrane
messages: Channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. May participate in the regulation of osmotic pressure changes within the cell.
messages: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.
messages: Homopentamer.
messages: Membrane

224308:BSU 00140
gene id: 224308:BSU_00140
uniprot id: A0A6M3Z6C9
uniprot id: P37529
names: Activity Regulation
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DCK/DGK family.
messages: Deoxyadenosine inhibits deoxycytidine phosphorylation and deoxycytidine inhibits deoxyadenosine phosphorylation. Deoxyadenosine/deoxycytidine kinase is inhibited by both dATP and dCTP.
messages: Homodimer.
messages: Plays an essential role in generating the deoxyribonucleotide precursors dATP and dCTP for DNA metabolism. The phosphate acceptor specificity is strict toward deoxyadenosine (dAdo) and deoxycytidine (dCyd). The specificity toward the sugar moiety of the nucleoside is less strict. Both 2-deoxyribose, ribose, and arabinose nucleosides are phosphorylated, although the 2-deoxyribonucleosides are preferred. The phosphate donor specificity is dependent on the deoxyribonucleoside substrate, but GTP is efficient with both deoxycytidine and deoxyadenosine. Only nucleoside triphosphates can act as phosphate donors.

224308:BSU 02820
gene id: 224308:BSU_02820
uniprot id: O34327
names: Activity Regulation
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Rap family.
messages: Contains a small N-terminal 3-helix bundle domain and a large C-terminal TPR domain, connected by a linker region.
messages: Cytoplasm
messages: Inhibited in vitro by the competence and sporulation stimulating factor (CSF), encoded by phrC (PubMed:23526881). However, CSF has at least three targets (RapB, RapC, and RapJ) and the physiological importance of RapJ inhibition by CSF is unknown (PubMed:23526881). Interaction with CSF induces a conformational change in RapJ (PubMed:23526881).
messages: Involved in the regulation of sporulation (Probable). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:21346797, PubMed:23526881).
messages: Monomer in solution (PubMed:23526881). Homodimer (PubMed:21346797).

224308:BSU 36820
gene id: 224308:BSU_36820
uniprot id: A0A6M3ZGY1
uniprot id: P37810
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase gamma chain family.
messages: Cell membrane
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex (By similarity) (PubMed:19717609). Interacts with FloT (PubMed:23651456).
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
messages: Membrane raft
messages: Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.

224308:BSU 15780
gene id: 224308:BSU_15780
uniprot id: A0A6M3ZE72
uniprot id: O34530
names: Cofactor
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Cytoplasm
messages: Has been described as essential (PubMed:9743119, PubMed:16485133, PubMed:18344364), but also as non-essential (PubMed:15828870). Cells have a slow growth phenotype (PubMed:15828870, PubMed:16485133, PubMed:18344364, PubMed:22544754). Disrupted strain grows as chains of filaments, a cell curvature phenotype is also present, resulting in long wavy cells or short curved rods (PubMed:15828870, PubMed:22544754). Depleted cells form aberrant, swollen cells (PubMed:16485133, PubMed:18344364). Depleted cells DNA staining shows fragmented and/or disturbed nucleoid segregation; effects are seen most in minimal E-medium (PubMed:16485133). Depleted cells have an irregular deposition of cell wall and 15% have abnormal septal cleavage planes (PubMed:18344364).
messages: In vitro phosphorylated mostly on Thr (with lower signal on Ser) by PrkC in the presence of poly-L-Lys or myelin basic protein, dephosphorylated by PrpC (PubMed:19246764). Most in vitro phosphorylation occurs on Thr-166, in vivo phosphorylation has not been detected, but it might vary during the cell cycle (PubMed:22544754).
messages: Monomer, but able to form dimers (PubMed:16485133). Associates with 30S ribosomal subunit; a phospho-mimetic mutation increases association (PubMed:22544754). Probably binds 16S rRNA.
messages: Monomer. Associates with 30S ribosomal subunit, binds 16S rRNA.
messages: One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.
messages: One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP (PubMed:16485133). GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation (PubMed:22544754). Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo (PubMed:15828870). Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes (PubMed:15828870). Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis (PubMed:18344364).

224308:BSU 22840
gene id: 224308:BSU_22840
uniprot id: A0A6M3ZGR7
uniprot id: P50743
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Associates with the 50S ribosomal subunit.
messages: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.
messages: Cofractionates with the 70S ribosome; association is stabilized by the non-hydrolyzable GTP analog GMPPNP, and inhibited by GTP or GDP.
messages: Essential for growth, it cannot be disrupted. Depletion experiments show that cells become longer and abnormally curved, with nucleoid condensation. Cells have many fewer 70S ribosomes; the large ribosomal subunit is 45S and is missing proteins L16, L27, L36 and interacts with 2 large non-ribosomal proteins.
messages: Estimated to be present at 7000 copies per cell.
messages: GTPase that plays an essential role in the late steps of ribosome biogenesis.

224308:BSU 34190
gene id: 224308:BSU_34190
uniprot id: A0A6M4JPD7
uniprot id: P71067
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the lactate permease family.
messages: Cell membrane
messages: Cells lacking this gene are markedly impaired (but not totally blocked) in growth on L-lactate minimal medium but not impaired in growth on glucose minimal medium.
messages: Is the principal permease for the uptake of L-lactate in B.subtilis.
messages: Membrane
messages: Uptake of L-lactate across the membrane. Can also transport D-lactate and glycolate.

224308:BSU 25830
gene id: 224308:BSU_25830
uniprot id: P45943
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rap family.
messages: Cytoplasm
messages: Involved in the regulation of sporulation (PubMed:10629174). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:10629174). Probably plays a dispensable role in the overall context of sporulation initiation (PubMed:10629174).
messages: Phosphatase activity is inhibited by the phosphatase regulator PhrE.
messages: Transcription is under the control of the ComA-ComP two-component system.

224308:BSU 21990
gene id: 224308:BSU_21990
uniprot id: P54162
names: Function
names: Similarity
messages: Belongs to the RNase H family. EbsB subfamily.
messages: Not known; does not have RNase H activity.

224308:BSU 22920
gene id: 224308:BSU_22920
uniprot id: P38490
names: Developmental Stage
names: Function
names: Induction
names: Similarity
messages: Belongs to the YpeB family.
messages: Expressed in the forespore during sporulation.
messages: Expression is sigma G-dependent.
messages: Required for spore cortex hydrolysis during germination. Appears to be required for either expression, localization, activation or function of SleB.

224308:BSU 00190
gene id: 224308:BSU_00190
uniprot id: A0A6M4JBU0
uniprot id: P09122
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DnaX/STICHEL family.
messages: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).
messages: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.
messages: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria.
messages: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

224308:BSU 33380
gene id: 224308:BSU_33380
uniprot id: A0A6M3ZJC0
uniprot id: O32208
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein ModA family.
messages: Cell membrane

224308:BSU 12570
gene id: 224308:BSU_12570
uniprot id: P39785
names: Function
names: Similarity
names: Subunit
messages: Dimer of a small and a large subunit.
messages: Functions as a terminase.
messages: To B.subtilis YqaS and B.subtilis phage SPP1 terminase small subunit.

224308:BSU 14060
gene id: 224308:BSU_14060
uniprot id: O34717
names: Function
names: Induction
names: Similarity
messages: Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA (By similarity).
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 26880
gene id: 224308:BSU_26880
uniprot id: A0A6M4JNT0
uniprot id: O07931
names: Similarity
messages: Belongs to the PrpF family.

224308:BSU 20000
gene id: 224308:BSU_20000
uniprot id: O31873
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 15020
gene id: 224308:BSU_15020
uniprot id: A0A6M4JG25
uniprot id: O34797
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial CoaD family.
messages: Cytoplasm
messages: Homohexamer.
messages: Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.

224308:BSU 14850
gene id: 224308:BSU_14850
uniprot id: A0A6M3ZAC2
uniprot id: O07639
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SEDS family. FtsW subfamily.
messages: Cell membrane
messages: Expression is regulated by YofA.
messages: Membrane
messages: Peptidoglycan polymerase that is essential for cell division.

224308:BSU 25570
gene id: 224308:BSU_25570
uniprot id: A0A6M3ZEB6
uniprot id: P39695
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Destabilization of ComFA (PubMed:17630974).
messages: Expression activated by ComK (PubMed:11948146).
messages: Membrane
messages: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding.
messages: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI.

224308:BSU 17210
gene id: 224308:BSU_17210
uniprot id: A0A6M3ZII4
uniprot id: O31782
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 3 phosphopantetheines covalently.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.
messages: The acyl carrier 1 domain binds alanine.

224308:BSU 26120
gene id: 224308:BSU_26120
uniprot id: P45923
names: Subunit
messages: Monomer.

224308:BSU 23640
gene id: 224308:BSU_23640
uniprot id: P54567
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 26890
gene id: 224308:BSU_26890
uniprot id: A0A6M3ZEB4
uniprot id: O07921
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
messages: Belongs to the glycosyl hydrolase 46 family.
messages: Secreted

224308:BSU 06140
gene id: 224308:BSU_06140
uniprot id: P39143
names: Function
messages: Activator of the glucitol dehydrogenase gene (gutB).

224308:BSU 31250
gene id: 224308:BSU_31250
uniprot id: A0A6M4JMY2
uniprot id: P39216
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase.
messages: Membrane

224308:BSU 18630
gene id: 224308:BSU_18630
uniprot id: O34918
names: Function
names: Subcellular Location Annotation
messages: May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.
messages: cell wall

224308:BSU 24010
gene id: 224308:BSU_24010
uniprot id: A0A6M4JNE3
uniprot id: P33449
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally diverse compounds as ethidium bromide, rhodamine and acridine dyes, tetraphenylphosphonium, puromycin, chloramphenicol, doxorubicin, and fluoroquinolone antibiotics.
messages: Membrane

224308:BSU 17170
gene id: 224308:BSU_17170
uniprot id: A0A6M4JII6
uniprot id: P40802
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the enoyl-CoA hydratase/isomerase family.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to PksL.

224308:BSU 00770
gene id: 224308:BSU_00770
uniprot id: A0A6M3Z6J5
uniprot id: P28822
names: Function
names: Similarity
messages: Belongs to the DHPS family.
messages: Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.

224308:BSU 13170
gene id: 224308:BSU_13170
uniprot id: O34598
names: Function
names: Induction
names: Similarity
messages: Belongs to the cytidine and deoxycytidylate deaminase family.
messages: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
messages: Expressed only during limited or partially limited nitrogen conditions. Can be induced to high levels in the presence of purines or intermediates of the purine catabolic pathway. Expression seems indirectly controlled by TnrA and GlnR.

224308:BSU 39370
gene id: 224308:BSU_39370
uniprot id: A0A6M4JQT4
uniprot id: P42084
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the metallo-dependent hydrolases superfamily. HutI family.
messages: Binds 1 zinc or iron ion per subunit.
messages: Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate. It is the third step in the universal histidine degradation pathway.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 18280
gene id: 224308:BSU_18280
uniprot id: O35015
names: Disruption Phenotype
names: Similarity
messages: Belongs to the glycosyl hydrolase-like 10 (GHL10) family.
messages: Cells lacking this gene show up-regulated sigma X activity.

224308:BSU 39610
gene id: 224308:BSU_39610
uniprot id: P54941
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Membrane raft
messages: Part of the ABC transporter complex FhuCBGD involved in iron(3+)-hydroxamate import. Binds the iron(3+)-hydroxamate complex and transfers it to the membrane-bound permease. Partially required for the transport of desferrioxamine.
messages: The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB and FhuG) and a solute-binding protein (FhuD or YxeB).

224308:BSU 36230
gene id: 224308:BSU_36230
uniprot id: A0A6M4JPV2
uniprot id: P96718
names: Activity Regulation
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.
messages: Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid.
messages: Competitively inhibited by UDP-glucose. Activated by phosphorylation, which may increase affinity for NAD(+); inhibited by dephosphorylation.
messages: Cytoplasm
messages: Phosphorylated on tyrosine residue(s). Phosphorylated by YwqD and dephosphorylated by YwqE in vitro.

224308:BSU 28280
gene id: 224308:BSU_28280
uniprot id: A0A6M4JMF7
uniprot id: P94565
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
messages: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
messages: Cytoplasm
messages: Homodimer.

224308:BSU 22080
gene id: 224308:BSU_22080
uniprot id: A0A6M3ZD50
uniprot id: P50848
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the peptidase M32 family.
messages: Binds 1 zinc ion per subunit.
messages: Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.
messages: Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues. Has lower activity with substrates ending with His or Trp.
messages: Homodimer.

224308:BSU 19620
gene id: 224308:BSU_19620
uniprot id: O34866
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M15B family.
messages: Cell membrane

224308:BSU 15910
gene id: 224308:BSU_15910
uniprot id: A0A6M4JMX5
uniprot id: P51831
names: Function
names: Similarity
names: Subunit
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.
messages: Homotetramer.

224308:BSU 00340
gene id: 224308:BSU_00340
uniprot id: P37543
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the methyltransferase superfamily.
messages: Methylates cellular RNA.
messages: No visible growth defects, DNA is still methylated on A-5 of 5'-GACGAG-3'.

224308:BSU 32420
gene id: 224308:BSU_32420
uniprot id: A0A6M3ZFS4
uniprot id: O32138
names: Function
names: Induction
names: Similarity
messages: Activates the expression of pucFG, pucH, pucI, pucJKLM and guaD, while it represses pucABCDE and its own expression.
messages: Belongs to the CdaR family.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression slightly decreases when allantoin is added during limiting-nitrogen conditions.

224308:BSU 03800
gene id: 224308:BSU_03800
uniprot id: A0A6M4JFE1
uniprot id: P94418
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Disruption mutants are unable to use petrobactin for iron delivery and growth.
messages: Membrane
messages: Part of the ABC transporter complex YclNOPQ involved in uptake of ferric-petrobactin. Petrobactin is a photoreactive 3,4-catecholate siderophore produced by many members of the B.cereus group, including B.anthracis. Probably responsible for the translocation of the substrate across the membrane.
messages: The complex is composed of two ATP-binding proteins (YclP), two transmembrane proteins (YclN and YclO) and a solute-binding protein (YclQ).

224308:BSU 24820
gene id: 224308:BSU_24820
uniprot id: P54498
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 34660
gene id: 224308:BSU_34660
uniprot id: A0A6M3ZGI9
uniprot id: O06984
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
messages: Cell membrane
messages: Membrane

224308:BSU 11200
gene id: 224308:BSU_11200
uniprot id: A0A6M4JF64
uniprot id: P36843
names: Activity Regulation
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ArgJ family.
messages: Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.
messages: Cytoplasm
messages: Feedback inhibition by L-arginine.
messages: Heterotetramer of two alpha and two beta chains.
messages: Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.
messages: Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.

224308:BSU 02900
gene id: 224308:BSU_02900
uniprot id: A0A6M4JF58
uniprot id: P80875
names: Induction
names: Similarity
messages: Belongs to the CAPAB/TerDEXZ family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 00810
gene id: 224308:BSU_00810
uniprot id: A0A6M3Z6G8
uniprot id: P37567
names: Function
names: Similarity
messages: Belongs to the Dus family.
messages: Belongs to the dus family.
messages: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.

224308:BSU 39580
gene id: 224308:BSU_39580
uniprot id: P54944
names: Miscellaneous
messages: Present in an increased level in yabG mutant spores.

224308:BSU 40490
gene id: 224308:BSU_40490
uniprot id: A0A6M4JR86
uniprot id: P37483
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 39 family.
messages: Cell membrane
messages: Membrane

224308:BSU 09900
gene id: 224308:BSU_09900
uniprot id: A0A6M4JEG5
uniprot id: O07523
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: To M.jannaschii MJ1024.

224308:BSU 06850
gene id: 224308:BSU_06850
uniprot id: O31510
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
messages: By SigK and GerE.
messages: Expression starts 5 hours after onset of sporulation and peaks at 6 hours after onset.
messages: No visible effect on vegetative growth, heat resistance of spores or their germination properties.
messages: Part of the spore coat.
messages: Present in an increased level in yabG mutant spores.
messages: Spore coat

224308:BSU 30135
gene id: 224308:BSU_30135
uniprot id: A0A6M4JMK3
uniprot id: C0SPB3
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Involved in pectin degradation (PubMed:29240795). Part of the ABC transporter complex YtcQP-YteP involved in the uptake of polygalacturonan and rhamnogalacturonan type I (PubMed:17449691, PubMed:29240795). Responsible for the translocation of the substrate across the membrane (Probable).
messages: Membrane
messages: The complex is probably composed of two ATP-binding proteins (MsmX), two transmembrane proteins (YtcP and YteP) and a solute-binding protein (YtcQ).
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 15830
gene id: 224308:BSU_15830
uniprot id: A0A6M3ZB26
uniprot id: O34318
names: Similarity
messages: Belongs to the asp23 family.

224308:BSU 41040
gene id: 224308:BSU_41040
uniprot id: A0A6M3ZJ85
uniprot id: Q01625
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the OXA1/ALB3/YidC family. Type 2 subfamily.
messages: Cell membrane
messages: Essential for sporulation, disruption affects the expression of prespore-specific genes but not early mother-cell-specific genes, i.e. cells do not progress beyond stage III of sporulation. A double spoIIIJ-yqjG deletion is lethal. Genetic competence increases about 20-fold in the single disruption.
messages: Membrane
messages: Mostly monomeric, it may also form dimers. Interacts with SpoIIIAE. Forms a complex with the F(1)F(0) ATP synthase in which can be found the alpha, beta, gamma, delta and epsilon subunits of F(1) and a, b and subunits of F(0). YqgA is found in the same complex.
messages: Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity). Also involved in protein secretion processes. Essential for sporulation by activating sigma factor SpoIIIG/SigG after engulfment is completed in the prespore, maybe by acting on SpoIIIAE. It has an overlapping, although partly distinct, function compared to YqjG(MisCB).
messages: Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins.
messages: SpoIIIJ is required only after engulfment and turned off at the onset of sporulation. While it is predominantly expressed in vegetative cells, its low expression after the onset of sporulation is essential for sporulation to occur.

224308:BSU 35920
gene id: 224308:BSU_35920
uniprot id: A0A6M4JPW2
uniprot id: P36945
names: Activity Regulation
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
messages: Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.
messages: Belongs to the carbohydrate kinase pfkB family.
messages: Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.

224308:BSU 30190
gene id: 224308:BSU_30190
uniprot id: A0A6M4JNY5
uniprot id: P53554
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the cytochrome P450 family.
messages: Binds 1 heme group covalently per subunit.
messages: Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid.

224308:BSU 28160
gene id: 224308:BSU_28160
uniprot id: A0A6M4JKP5
uniprot id: P16645
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: Required for HemL synthesis.
messages: To M.leprae U1620K.

224308:BSU 14840
gene id: 224308:BSU_14840
uniprot id: A0A6M4JIB3
uniprot id: O07638
names: Function
names: Similarity
messages: Belongs to the UPF0358 family.
messages: Essential for cell growth and for normal cell shape.

224308:BSU 13950
gene id: 224308:BSU_13950
uniprot id: A0A6M4JHN7
uniprot id: P54576
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the methyl-accepting chemotaxis (MCP) protein family.
messages: Cell membrane
messages: Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpC is required for taxis to cysteine, proline, threonine, glycine, serine, lysine, valine and arginine and for aspartate, glutamine, histidine and glutamate. Primarily mediates response to positive stimulus of PTS carbohydrates. Greatly influences the duration or magnitude of the response to negative PTS carbohydrate stimulus.
messages: Induced by SigD. Expression increases in the late-exponential growth-phase and is maximal during the early-stationary phase.
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft
messages: Some glutamine residues are deamidated to glutamate by CheD and subsequently methylated.

224308:BSU 23130
gene id: 224308:BSU_23130
uniprot id: A0A6M3ZD81
uniprot id: P35162
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
messages: Cell membrane
messages: Cells lacking this gene exhibit a lack of cytochromes c but normal contents of cytochrome a and b. Can sporulate.
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft
messages: Required for the biogenesis of c-type cytochromes.

224308:BSU 03040
gene id: 224308:BSU_03040
uniprot id: P00691
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 13 family.
messages: Binds 2 calcium ions per subunit.
messages: Monomer.
messages: Secreted

224308:BSU 40310
gene id: 224308:BSU_40310
uniprot id: O32295
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Phr family.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably subject to only one processing event, at the N-terminal end of the mature peptide.
messages: Cytoplasm
messages: Disruption of the gene results in the reduction of aprE expression.
messages: Part of the rapG-phrG operon (PubMed:12950930). Transcription of phrG only is controlled by the sigma-H factor (PubMed:12950930). Expression is rapidly induced in the transition state to stationary phase (PubMed:12950930).
messages: Secreted
messages: Signaling molecule involved in the regulation of expression of DegU-controlled genes (PubMed:12950930). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (By similarity). Stimulates the DegU-dependent expression of aprE, an extracellular alkaline protease (PubMed:12950930). Acts by inhibiting RapG activity (PubMed:12950930). At high concentrations, represses the DegS-dependent aprE expression (PubMed:12950930).

224308:BSU 31420
gene id: 224308:BSU_31420
uniprot id: O05235
names: Similarity
messages: Belongs to the DmpD/TodF/XylF esterase family.

224308:BSU 12430
gene id: 224308:BSU_12430
uniprot id: Q00828
names: Activity Regulation
names: Cofactor
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Rap family.
messages: Can also use Mg(2+) and Ca(2+), with lower efficiency.
messages: Cytoplasm
messages: Homodimer (PubMed:11923303). Interacts with its substrate, phosphorylated Spo0F, and its inhibitor, the PhrA pentapeptide (PubMed:11923303, PubMed:22267516). The RapA dimer forms a stable complex with two molecules of phosphorylated Spo0F (PubMed:11923303). The complex is dissociated after dephosphorylation of Spo0F by RapA (PubMed:11923303).
messages: Involved in the regulation of sporulation (PubMed:1624431, PubMed:8001132, PubMed:11923303, PubMed:22267516). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:8001132, PubMed:11923303, PubMed:22267516).
messages: Phosphatase activity is inhibited by the phosphatase regulator PhrA (PubMed:8643670, PubMed:9238025, PubMed:11923303, PubMed:22267516). Interaction with PhrA dissociates the RapA-Spo0F complex (PubMed:11923303). Activity is abolished in the presence of EDTA (PubMed:11923303).
messages: The N-terminal half of RapA contains the necessary structural determinants for substrate binding and enzymatic activity, and the C-terminal half contains the binding site for PhrA peptide (PubMed:22267516). The central TPR3 to TPR5 repeats provide the binding specificity toward the Phr peptide inhibitor (PubMed:22267516).
messages: Under all conditions of nutrient limitation, and by addition of decoyinine.

224308:BSU 38740
gene id: 224308:BSU_38740
uniprot id: A0A6M3ZHX5
uniprot id: P94366
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Cysteine exporter (TC 3.A.1.129.1) family.
messages: Cell membrane
messages: Forms a heterodimer with CydD.
messages: In CydC the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.
messages: Membrane
messages: Part of the ABC transporter complex CydDC that exports the reduced low-molecular-weight thiols cysteine and glutathione from the cell. Export of these thiol-containing redox-active molecules may be crucial for redox homeostasis, permitting correct assembly of various respiratory complexes and formation of correct disulfide bonds in secreted proteins. CydC contains transmembrane domains (TMD), which form a pore in the membrane, and an ATP-binding domain (NBD), which is responsible for energy generation.

224308:BSU 25700
gene id: 224308:BSU_25700
uniprot id: P54451
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 07320
gene id: 224308:BSU_07320
uniprot id: A0A6M3Z885
uniprot id: O06481
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 13860
gene id: 224308:BSU_13860
uniprot id: O31689
names: Cofactor
names: Similarity
messages: Belongs to the peptidase M24B family.
messages: Binds 2 manganese ions per subunit.

224308:BSU 28300
gene id: 224308:BSU_28300
uniprot id: A0A6M3ZF39
uniprot id: P37252
names: Function
names: Similarity
names: Subunit
messages: Belongs to the acetolactate synthase small subunit family.
messages: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.
messages: Dimer of large and small chains.

224308:BSU 13960
gene id: 224308:BSU_13960
uniprot id: O34948
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the HIBADH-related family.
messages: Cell membrane
messages: Membrane raft

224308:BSU 38890
gene id: 224308:BSU_38890
uniprot id: P55182
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00510
gene id: 224308:BSU_00510
uniprot id: A0A6M3Z6R1
uniprot id: P14193
names: Activity Regulation
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Activated by inorganic phosphate, and to a lesser extent by sulfate ions (PubMed:2169413). In addition to form a complex with ATP, Mg(2+) also acts as a cofactor (PubMed:2169413). Strongly inhibited by ADP through competitive binding at the activation site and at a specific allosteric site (PubMed:2169413, PubMed:16008562). Less strongly inhibited by alpha,beta-methylene ATP (mADP), AMP, GDP, GMP and UTP (PubMed:2169413, PubMed:16008562).
messages: Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.
messages: Binds 2 Mg(2+) ions per subunit.
messages: Binds 2 Mg(2+) ions per subunit. Each Mg(2+) binds only one residue (His-136 and Asp-175, respectively) of this protein, however the magnesium ions also bind substrates and water molecules to complete their coordination spheres (PubMed:2169413, PubMed:11790837). Can also use Mn(2+) and Cd(2+) ions, but the activity is less than that obtained with Mg(2+) ions (PubMed:2169413, PubMed:11790837).
messages: Cytoplasm
messages: Homohexamer.
messages: Homohexamer; trimer of dimers.
messages: Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).
messages: This enzyme uses a steady state ordered mechanism, where Mg(2+) binds first, followed by Mg-ATP and lastly, ribose 5-phosphate.

224308:BSU 34050
gene id: 224308:BSU_34050
uniprot id: A0A6M4JPD1
uniprot id: O07020
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the LutA/YkgE family.
messages: Cells lacking this gene are unable to grow on minimal medium with L-lactate as the sole carbon source. Cells lacking the lutABC operon exhibit little or no defect in biofilm formation on MSgg medium, but form small colonies that almost completely lacked surface architecture when glycerol is replaced with L-lactate in the MSgg medium.
messages: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. May also allow cells to utilize an alternative carbon source during biofilm formation, since it contributes to the formation of architecturally complex communities when lactate is present.
messages: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source.
messages: Is under the dual control of the transcriptional repressors LutR and SinR, which allows the lutABC operon to be induced during both growth in liquid culture and biofilm formation. Is induced by L-lactate, which relieves LutR repression.

224308:BSU 17020
gene id: 224308:BSU_17020
uniprot id: O31779
names: Function
messages: May work together with YlbF to regulate community development.

224308:BSU 33610
gene id: 224308:BSU_33610
uniprot id: A0A6M3ZKU9
uniprot id: O32231
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs (By similarity). Involved in degradation of type I toxin-antitoxin system bsrG/SR4 RNAs (PubMed:22229825).
messages: 3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.
messages: Belongs to the RNR ribonuclease family. RNase R subfamily.
messages: Constitutively expressed, part of a 5 gene operon with multiple promoters. Not ethanol-stress induced.
messages: Cytoplasm
messages: No visible phenotype (PubMed:17369301). Increased half-life of type I toxin-antitoxin system RNAs of BsrG/SR4 (PubMed:22229825).

224308:BSU 29930
gene id: 224308:BSU_29930
uniprot id: C0SPA0
names: Similarity
messages: Belongs to the glycosyl hydrolase 13 family.

224308:BSU 40100
gene id: 224308:BSU_40100
uniprot id: A0A6M3ZHX8
uniprot id: P42974
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.
messages: Binds 1 FAD per subunit.
messages: Cell membrane
messages: Homodimer.
messages: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

224308:BSU 36310
gene id: 224308:BSU_36310
uniprot id: A0A6M4JQ09
uniprot id: C0SPB6
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Homotetramer (PubMed:16549871).
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Lacks the C-terminal region present in SsbA and thus is probably not able to participate in DNA replication and/or DNA repair.
messages: Not essential for replication of the chromosome, but is required for optimal competence (PubMed:14762004). Binds ssDNA, binding is facilitated by DprA, acts as an accessory factor for homologous DNA strand exchange (PubMed:25138221).
messages: Only expressed during growth in minimal medium, strongly induced when cells enter stationary phase with highest levels reached about 2 hours after transition to stationary phase (PubMed:14762004). Expression activated by ComK (PubMed:11918817, PubMed:11948146, PubMed:14762004).
messages: Phosphorylated by YwqD, which increases ssDNA affinity; dephosphorylated by YwqE.
messages: Preferentially expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:11918817, PubMed:16009133, PubMed:17630974).
messages: Transformation efficiency drops 10- to 50-fold (PubMed:11918817, PubMed:11948146, PubMed:14762004). Destabilization of DprA (PubMed:17630974).

224308:BSU 16590
gene id: 224308:BSU_16590
uniprot id: A0A6M4JGT3
uniprot id: P32726
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RimP family.
messages: Cytoplasm
messages: Required for maturation of 30S ribosomal subunits.

224308:BSU 29620
gene id: 224308:BSU_29620
uniprot id: A0A6M3ZFH9
uniprot id: O34411
names: Similarity
messages: Belongs to the PHP hydrolase family. HisK subfamily.

224308:BSU 37840
gene id: 224308:BSU_37840
uniprot id: A0A6M3ZIA6
uniprot id: P39629
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the glucose-1-phosphate thymidylyltransferase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.

224308:BSU 30910
gene id: 224308:BSU_30910
uniprot id: A0A6M4JKT8
uniprot id: P46915
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

224308:BSU 27280
gene id: 224308:BSU_27280
uniprot id: A0A6M4JJS5
uniprot id: O32029
names: Function
names: Induction
names: Similarity
messages: Belongs to the methyltransferase superfamily. YrrT family.
messages: By methionine. Repressed by sulfate and cysteine.
messages: Could be a S-adenosyl-L-methionine-dependent methyltransferase.

224308:BSU 40570
gene id: 224308:BSU_40570
uniprot id: A0A6M4JR54
uniprot id: P37489
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Phthalate permease family.
messages: Cell membrane
messages: Membrane

224308:BSU 18640
gene id: 224308:BSU_18640
uniprot id: O34343
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0700 family.
messages: Cell membrane

224308:BSU 35650
gene id: 224308:BSU_35650
uniprot id: Q02115
names: Caution
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the LytR/CpsA/Psr (LCP) family.
messages: Cell membrane
messages: Interacts with MreB (PubMed:21964069). Interacts with FloT (PubMed:23651456).
messages: May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).
messages: Membrane raft
messages: Single mutant has no effect on cell growth or morphology under normal growth conditions. Triple disruption of tagTUV genes is not viable (PubMed:21964069). Cells lacking this gene display a considerably increased transcription frequency of lytR and lytABC operon expression (PubMed:1357079).
messages: This protein is unrelated to LytR from S.aureus, which is part of a two-component regulatory system that, together with LytS, is involved in autolysis and cell wall metabolism. The B.subtilis ortholog of S.aureus LytR is LytT (AC P94514).
messages: Was originally thought to be involved in transcriptional regulation.

224308:BSU 29460
gene id: 224308:BSU_29460
uniprot id: A0A6M3ZFG6
uniprot id: O34457
names: Function
names: Similarity
messages: Belongs to the MoaB/Mog family.
messages: May be involved in the biosynthesis of molybdopterin.

224308:BSU 10080
gene id: 224308:BSU_10080
uniprot id: O07599
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 40139
gene id: 224308:BSU_40139
uniprot id: C0H3T8
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 14520
gene id: 224308:BSU_14520
uniprot id: A0A6M4JLE8
uniprot id: P39761
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.

224308:BSU 23670
gene id: 224308:BSU_23670
uniprot id: P54564
names: Similarity
messages: In the C-terminal section; belongs to the UPF0157 (GrpB) family.

224308:BSU 31290
gene id: 224308:BSU_31290
uniprot id: A0A6M4JNU2
uniprot id: O05242
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 13 family.
messages: Cytoplasm

224308:BSU 32920
gene id: 224308:BSU_32920
uniprot id: A0A6M3ZGF8
uniprot id: O32186
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 27690
gene id: 224308:BSU_27690
uniprot id: O32051
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25230
gene id: 224308:BSU_25230
uniprot id: A0A6M4JMF6
uniprot id: P17868
names: Similarity
messages: Belongs to the UPF0178 family.

224308:BSU 17180
gene id: 224308:BSU_17180
uniprot id: A0A6M3ZBD0
uniprot id: P40806
names: Caution
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 5 phosphopantetheines covalently.
messages: Cytoplasm
messages: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.
messages: The acyl carrier 2 domain binds glycine.
messages: Was originally thought to be two separate ORFs named pksJ and pksK.

224308:BSU 19410
gene id: 224308:BSU_19410
uniprot id: A0A6M4JJ16
uniprot id: O31852
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Cell surface
messages: LysM domains are thought to be involved in peptidoglycan binding.
messages: Probably functions as a cell separation enzyme in addition to LytE and LytF.
messages: The cell shape is not significantly different from that of the wild-type.
messages: Transcribed during the late vegetative and early stationary phases.

224308:BSU 20150
gene id: 224308:BSU_20150
uniprot id: O31884
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 27820
gene id: 224308:BSU_27820
uniprot id: A0A6M3ZIR3
uniprot id: P94447
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TACO1 family.
messages: Cytoplasm

224308:BSU 34850
gene id: 224308:BSU_34850
uniprot id: O06965
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Overall loss in complex colony architecture and lack of fruiting body-like structures.
messages: Required for complex colony architecture.
messages: Up-regulated by DegU and Spo0A. Repressed by AbrB.

224308:BSU 15480
gene id: 224308:BSU_15480
uniprot id: A0A6M4JJU0
uniprot id: P39766
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
messages: Cell membrane
messages: Membrane
messages: Transport of uracil in the cell.

224308:BSU 31570
gene id: 224308:BSU_31570
uniprot id: A0A6M4JNU8
uniprot id: O05255
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: Part of an ABC transporter complex involved in the uptake of guanosine (PubMed:21926227). Responsible for the translocation of the substrate across the membrane (Probable). May be a nucleoside transporter of broad specificity but with various affinities for different substrates (PubMed:21926227).
messages: The complex is composed of two ATP-binding proteins (NupO), two transmembrane proteins (NupP and NupQ) and a solute-binding protein (NupN).
messages: Transcriptionally regulated by CodY.

224308:BSU 25670
gene id: 224308:BSU_25670
uniprot id: P54453
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the TRAFAC class YlqF/YawG GTPase family.
messages: Binds GTP and GDP.
messages: Essential for growth, it cannot be disrupted. In depletion experiments cells become 1.5 to 2-fold longer and nucleoid distribution is dispersed. The number of replication origins increases, suggesting an increase in chromosome replication.
messages: Estimated to be present at 2000 copies per cell.

224308:BSU 06060
gene id: 224308:BSU_06060
uniprot id: A0A6M4JFI4
uniprot id: O34939
names: Activity Regulation
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: A methylase, recognizes the double-stranded sequence 5'-YTCGAR-3', methylates C-3 on both strands, and protects the DNA from cleavage by the BsuMI endonuclease.
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
messages: Constitutively expressed during exponential growth. Encoded in an operon with ydiP and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible.
messages: Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025, YdjA, YdiS and YdiR respectively) has been disrupted (PubMed:11751814). Triple deletions ydiO-ydiP-ydiR, ydiO-ydiP-ydiS and ydiO-ydiP-ydjA lead to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).
messages: Monomer (PubMed:3150363). May form a complex with YdiP, also seems to be active alone (Probable).
messages: Not expressed during sporulation.
messages: Somewhat inhibited by MgCl(2) and spermidine, strongly inhibited by MnCl(2).
messages: The characterized enzyme was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.

224308:BSU 19100
gene id: 224308:BSU_19100
uniprot id: O34920
names: Subcellular Location Annotation
messages: Cytoplasm

224308:BSU 08510
gene id: 224308:BSU_08510
uniprot id: A0A6M3Z8Q0
uniprot id: O31574
names: Similarity
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family. SDR39U1 subfamily.

224308:BSU 11300
gene id: 224308:BSU_11300
uniprot id: A0A6M3ZCW9
uniprot id: O32436
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the BMP lipoprotein family.
messages: Cell membrane
messages: Positive activator of the comK gene.

224308:BSU 12520
gene id: 224308:BSU_12520
uniprot id: P39781
names: Similarity
messages: To B.subtilis YqaL.

224308:BSU 30035
gene id: 224308:BSU_30035
uniprot id: A0A6M3ZIK3
uniprot id: O32068
names: Similarity
messages: Belongs to the pseudouridine synthase RsuA family.

224308:BSU 03981
gene id: 224308:BSU_03981
uniprot id: A0A6M4JF07
uniprot id: P42956
names: Caution
names: Domain
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Homodimer.
messages: Membrane
messages: The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II CmtAB PTS system is involved in D-mannitol transport.
messages: Was originally thought to be a longer ORF that encodes what is now known to be mtlA and mtlF.

224308:BSU 40670
gene id: 224308:BSU_40670
uniprot id: A0A6M4JRA4
uniprot id: P37499
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 24540
gene id: 224308:BSU_24540
uniprot id: P54510
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 24800
gene id: 224308:BSU_24800
uniprot id: P54500
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 11790
gene id: 224308:BSU_11790
uniprot id: O31623
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0713 family.
messages: Cell membrane
messages: Expressed during sporulation.
messages: Involved in sporulation.

224308:BSU 37700
gene id: 224308:BSU_37700
uniprot id: A0A6M3ZHT1
uniprot id: P39642
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Drug:H(+) antiporter-3 (DHA3) (TC 2.A.1.21) family.
messages: Cell membrane
messages: Part of the bacilysin biosynthesis operon. May be involved in self-resistance to bacilysin by permitting efflux of this antibiotic.
messages: The compound ppGpp is essential for the transcription of the bacABCDE operon and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 06600
gene id: 224308:BSU_06600
uniprot id: A0A6M4JE05
uniprot id: O34790
names: Caution
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the GGGP/HepGP synthase family. Group I subfamily.
messages: Cells lacking this gene show a cloggy growth and do not produce the dephosphorylated and acetylated derivatives of HepGP.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales.
messages: Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. To a much lesser extent, is also able to use geranyl diphosphate (GPP; C10) and geranylgeranyl diphosphate (GGPP; C20) as the prenyl donors, but not farnesyl pyrophosphate (FPP; C15). Cannot use glycerol-3-phosphate (G3P) or 3-phosphoglycerate (3PG) as an acceptor.
messages: The HepGP product, which is the first archaea-type G1P-based ether lipid being identified within the phylogenetic domain of the bacteria, was found to be subsequently dephosphorylated and acetylated in vivo. However, HepG and its acetylated derivatives represent only a minor fraction of the total lipid in B.subtilis.

224308:BSU 10510
gene id: 224308:BSU_10510
uniprot id: Q796S4
names: Subcellular Location Annotation
messages: Cytoplasm

224308:BSU 30920
gene id: 224308:BSU_30920
uniprot id: O34656
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotS family.
messages: Spore coat

224308:BSU 23760
gene id: 224308:BSU_23760
uniprot id: A0A6M4JKH1
uniprot id: P54556
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the prokaryotic pantothenate kinase family.
messages: Cytoplasm

224308:BSU 34580
gene id: 224308:BSU_34580
uniprot id: O06992
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Could have a role in maltodextrin utilization.

224308:BSU 12160
gene id: 224308:BSU_12160
uniprot id: O34720
names: Cofactor
names: Similarity
messages: Binds 1 [2Fe-2S] cluster.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
messages: Binds 4 [4Fe-4S] clusters.
messages: In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

224308:BSU 24560
gene id: 224308:BSU_24560
uniprot id: A0A6M3ZDL8
uniprot id: P54376
names: Function
names: Similarity
names: Subunit
messages: Belongs to the GcvP family. N-terminal subunit subfamily.
messages: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity).
messages: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.
messages: The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.

224308:BSU 08690
gene id: 224308:BSU_08690
uniprot id: A0A6M3Z958
uniprot id: P71082
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Homodimer.
messages: May be involved in multidrug export. Transmembrane domains (TMD) form a pore in the cell membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
messages: Membrane
messages: The ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

224308:BSU 07500
gene id: 224308:BSU_07500
uniprot id: A0A6M3ZDN7
uniprot id: O34832
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of one ATP-binding protein (YfmF), two transmembrane proteins (YfmD and YfmE) and a solute-binding protein (YfmC).

224308:BSU 12960
gene id: 224308:BSU_12960
uniprot id: A0A6M3ZIL0
uniprot id: P26906
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: A null mutation abolishes the ability of a proline auxotroph to grow in a medium containing the dipeptide Pro-Gly as sole proline source.
messages: Belongs to the bacterial solute-binding protein 5 family.
messages: Cell membrane
messages: Expressed early during sporulation.
messages: Membrane
messages: Nutrient deficiency conditions.
messages: Probably part of the ABC transporter DppBCDE involved in dipeptide transport.

224308:BSU 00230
gene id: 224308:BSU_00230
uniprot id: P24282
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Forespore outer membrane
messages: Forms a complex with SpoIVFA and SpoIVFB localized in the mother cell membrane surrounding the forespore.
messages: Involved in the mediation of the intercompartmental coupling of pro-sigma K processing to events in the forespore. Inhibits SpoIVFB-processing activity until a signal has been received from the forespore. Could inhibit SpoIVFB metalloprotease activity by coordinating a zinc in the SpoIVFB active site, preventing access of a water molecule and the sequence of pro-sigma K, which are necessary for peptide bond hydrolysis to produce sigma-K.

224308:BSU 34440
gene id: 224308:BSU_34440
uniprot id: P32959
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the beta-lactamase family.
messages: Expressed during sporulation. Detected in mature spores (PubMed:3080407).
messages: Forespore outer membrane
messages: Probably involved in peptidoglycan modification during cortex synthesis.

224308:BSU 31510
gene id: 224308:BSU_31510
uniprot id: O05249
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 05109
gene id: 224308:BSU_05109
uniprot id: C0H3V7
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 00050
gene id: 224308:BSU_00050
uniprot id: P37525
names: Disruption Phenotype
names: Function
messages: Mutation impairs pellicle formation, complex colony architecture and motility inhibition in a sinR mutant background.
messages: Regulates the biosynthesis of the extracellular matrix and the biofilm formation. May act as an enhancer of biofilm gene expression. Acts in parallel to the pathway that governs SinR derepression.

224308:BSU 02560
gene id: 224308:BSU_02560
uniprot id: P42245
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YcbM/YcbL. Probably activates YcbL by phosphorylation.

224308:BSU 35820
gene id: 224308:BSU_35820
uniprot id: A0A6M3ZGZ6
uniprot id: P39571
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKC lipoprotein family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Involved in the response to the germinative mixture of L-asparagine, glucose, fructose and potassium ions (AGFK). Cannot stimulate germination in the absence of gerD and gerK gene products (fructose and glucose receptors respectively).

224308:BSU 40259
gene id: 224308:BSU_40259
uniprot id: C0H3U0
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 26830
gene id: 224308:BSU_26830
uniprot id: O05410
names: Similarity
messages: Belongs to the calycin superfamily. ZinT family.

224308:BSU 20020
gene id: 224308:BSU_20020
uniprot id: O34919
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Belongs to the dUTPase family.
messages: Binds 1 Mg(2+) per subunit, coordinated entirely by the nucleotide and ordered water molecules.
messages: Homotrimer.
messages: Involved in nucleotide metabolism: produces dUMP, the immediate precursor of thymidine nucleotides and decreases the intracellular concentration of dUTP, so that uracil cannot be incorporated into DNA (PubMed:9679200). The Ser-62 side chain changes its position upon ligand-binding to make contacts with the nucleotide phosphates (PubMed:21358047).
messages: No visible phenotype; double yncF-yosS deletions (both encode dUTPases) are also viable.
messages: The uracil deoxyribose moiety interacts with the protein through Ile-79 and Tyr-83, which discriminate against the ribose form of the nucleotide.

224308:BSU 13700
gene id: 224308:BSU_13700
uniprot id: A0A6M4JJC3
uniprot id: O31673
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subunit
messages: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus a role in overall protein quality control in response to heat stress.
messages: Belongs to the ClpA/ClpB family.
messages: Belongs to the ClpA/ClpB family. ClpE subfamily.
messages: By heat shock via the relief of repression carried out by the transcriptional regulator CtsR.
messages: Cells lacking this gene display a retardation of CtsR-dependent gene induction as well as a delayed restoration of the repressed stage. They also show a significant delay in disaggregation of heat-generated insoluble protein aggregates.
messages: ClpE is a very short-lived protein, that is, at least in the soluble cell fraction, degraded mainly by ClpCP.
messages: Interacts with ClpP.

224308:BSU 33790
gene id: 224308:BSU_33790
uniprot id: O32242
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By Spo0A during nutrient starvation through its direct negative control of AbrB, repressed by AbrB during regular growth when nutrients are plentiful (PubMed:16469701). Represses its own transcription (PubMed:16469701). Induced by expression of SDP (active peptide of sdpC) (PubMed:16469701, PubMed:23687264).
messages: Cytoplasm
messages: Represses the transcription of the sdpIR operon and of several other operons that probably contribute to delaying commitment to sporulation.

224308:BSU 11980
gene id: 224308:BSU_11980
uniprot id: O31642
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 28010
gene id: 224308:BSU_28010
uniprot id: A0A6M3ZJQ2
uniprot id: Q01467
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MreD family.
messages: Cell membrane
messages: Involved in formation of the rod shape of the cell. May also contribute to regulation of formation of penicillin-binding proteins (By similarity).
messages: Membrane

224308:BSU 01240
gene id: 224308:BSU_01240
uniprot id: A0A6M4JES5
uniprot id: P12873
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL29 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 02390
gene id: 224308:BSU_02390
uniprot id: O31461
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
messages: Inhibited by canaline.
messages: Transaminates branched-chain amino acids and ketoglutarate. Involved in the final step of the methionine regeneration pathway, where ketomethiobutyrate (KMTB) is converted to methionine via a transamination. The amino donor preference is isoleucine, leucine, valine, phenylalanine, and tyrosine.

224308:BSU 06590
gene id: 224308:BSU_06590
uniprot id: A0A6M3Z825
uniprot id: O34849
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glutamate synthase family.
messages: Cell membrane

224308:BSU 36260
gene id: 224308:BSU_36260
uniprot id: A0A6M3ZHD8
uniprot id: P96715
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CpsC/CapA family.
messages: Cell membrane
messages: Membrane
messages: Not phosphorylated in vitro by YwqD.
messages: Required for YwqD kinase activity. May bring YwqD and its substrates into contact. Probably involved in the regulation of capsular polysaccharide biosynthesis.

224308:BSU 34820
gene id: 224308:BSU_34820
uniprot id: A0A6M3ZGX4
uniprot id: O06967
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: 50% inhibited by vanadate; it has been suggested that vanadate fully inhibits the dimer but not the monomer (PubMed:15766260). Activated by 15 uM reserpine then inhibited by higher concentrations.
messages: An efflux transporter able to transport Hoechst 33342, ethidium bromide, doxorubicin and a number of other drugs in vitro into inside out vesicles. The endogenous substrate is unknown. It has been suggested that NBD dimerization induced by ATP-binding causes a large conformational change responsible for substrate translocation (PubMed:18215075). Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (Probable).
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Homodimer.
messages: In BmrA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused. This is considered to be a half-size transporter that undergoes homodimerization to be functional.
messages: Membrane
messages: Not essential.

224308:BSU 14720
gene id: 224308:BSU_14720
uniprot id: O07626
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 10620
gene id: 224308:BSU_10620
uniprot id: A0A6M4JEP0
uniprot id: P23477
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: At low magnesium concentrations there is no nuclease activity, but helicase activity is unaffected.
messages: Belongs to the helicase family. AddB/RexB type 1 subfamily.
messages: Binds 1 [4Fe-4S] cluster.
messages: Heterodimer of AddA and AddB.
messages: The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.
messages: The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity.
messages: This enzyme is a functional homolog of the RecBCD enzyme; unlike the RecBCD enzyme it degrades both duplex strands symmetrically.

224308:BSU 16510
gene id: 224308:BSU_16510
uniprot id: A0A6M3ZB56
uniprot id: O31749
names: Activity Regulation
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allosterically activated by GTP. Can also be activated by dGTP and 3'-anthraniloyl-2'-deoxyguanosine-5'-triphosphate (Ant-dGTP). Inhibited by UTP, 5-bromo-UTP and 5-iodo-UTP.
messages: Allosterically activated by GTP. Inhibited by UTP.
messages: Belongs to the UMP kinase family.
messages: Catalyzes the reversible phosphorylation of UMP to UDP, with ATP or dATP as the most efficient phosphate donors. Is also able to phosphorylate 5-fluoro-UMP and 6-aza-UMP.
messages: Catalyzes the reversible phosphorylation of UMP to UDP.
messages: Cytoplasm
messages: Homohexamer (PubMed:12869195). Interacts with BrxC (PubMed:33722570).
messages: Homohexamer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: The peripheral distribution of PyrH is related most probably to its role in the synthesis of membrane sugar components and its putative role in cell division.

224308:BSU 34620
gene id: 224308:BSU_34620
uniprot id: O06988
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Cytoplasm
messages: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferring glucose or maltose to another moiety of sugars by forming alpha-(1,6)- and alpha-(1,3)-glycosidic linkages upon the hydrolysis of substrate at concentrations of 5% or higher (By similarity).
messages: Monomer or homodimer; in equilibrium.

224308:BSU 01370
gene id: 224308:BSU_01370
uniprot id: A0A6M3Z795
uniprot id: P16304
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the adenylate kinase family.
messages: By superoxide.
messages: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.
messages: Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.
messages: Cytoplasm
messages: Monomer.

224308:BSU 14150
gene id: 224308:BSU_14150
uniprot id: A0A6M3ZA71
uniprot id: O34737
names: Function
names: Similarity
messages: Belongs to the flavodoxin family.
messages: Low-potential electron donor to a number of redox enzymes.

224308:BSU 35850
gene id: 224308:BSU_35850
uniprot id: P96741
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. Cof family.
messages: Catalyzes the dephosphorylation of the riboflavin precursor 5-amino-6-(5-phospho-D-ribitylamino)uracil and of flavin mononucleotide (FMN) in vitro (PubMed:26316208). Also catalyzes the dephosphorylation of phosphorylated 5-6 carbon sugars and monophosphate nucleotides (NMP) in vitro (PubMed:25848029).

224308:BSU 17520
gene id: 224308:BSU_17520
uniprot id: P94482
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 15690
gene id: 224308:BSU_15690
uniprot id: A0A6M4JGK7
uniprot id: O35011
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RNA polymerase subunit omega family.
messages: Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits (By similarity). In vitro reconstitution experiments this subunit is dispensible (PubMed:18289874).
messages: Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

224308:BSU 12250
gene id: 224308:BSU_12250
uniprot id: O34756
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 40870
gene id: 224308:BSU_40870
uniprot id: P37517
names: Function
names: Subunit
messages: Seems to be complexed to phosphorylated HPr.
messages: Transcriptional regulator involved in catabolite repression of several operons.

224308:BSU 25450
gene id: 224308:BSU_25450
uniprot id: A0A6M4JNG9
uniprot id: P54460
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyltransferase superfamily. PrmA family.
messages: Cytoplasm
messages: Methylates ribosomal protein L11.

224308:BSU 35050
gene id: 224308:BSU_35050
uniprot id: O34692
names: Subcellular Location Annotation
messages: Cytoplasm

224308:BSU 18320
gene id: 224308:BSU_18320
uniprot id: A0A6M4JMC0
uniprot id: P39847
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 2 phosphopantetheines covalently.
messages: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Ala/Val as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Ala/Val residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.

224308:BSU 36840
gene id: 224308:BSU_36840
uniprot id: A0A6M3ZH54
uniprot id: P37811
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase delta chain family.
messages: Cell membrane
messages: F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex (By similarity) (PubMed:19717609) (Probable). Interacts with FloT (PubMed:23651456).
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Membrane raft
messages: This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.

224308:BSU 14240
gene id: 224308:BSU_14240
uniprot id: O34857
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expression is constant during late log phase and for at least 2 hours in stationary phase (at protein level) (PubMed:11849533). Probably repressed by AbrB and SinR, as well as partially by ComK (PubMed:11849533). Probably negatively regulates its own expression (PubMed:11849533, PubMed:21085634). There are 1000-3000 proteins/chromosome in exponential phase grown in minimal medium (PubMed:21085634).
messages: Increased expression of comK, increases transformation about 5-fold, decreases sporulation efficiency about 5-fold (PubMed:11849533). Increased excision of mobile element ICEBs1 (PubMed:21085634).
messages: Repressor of comK, the master regulator of competence development (PubMed:11849533). Overexpression seems to be lethal (PubMed:11849533). Represses at least 20 genes that specify membrane-localized and secreted proteins, including some that encode products with antibiotic activity (PubMed:15743949). Binds to many AT-rich sites in the chromosome, many of which are known or thought to derive from horizontal gene transfer; helps keep mobile element ICEBs1 quiescent in the genome (PubMed:21085634). Binds to its own promoter and is thus probably autoregulatory (PubMed:21085634).
messages: The C-terminus (residues 95-191) binds DNA (PubMed:21085634).
messages: nucleoid

224308:BSU 29750
gene id: 224308:BSU_29750
uniprot id: P39912
names: Similarity
messages: Belongs to the class-I DAHP synthase family.

224308:BSU 25860
gene id: 224308:BSU_25860
uniprot id: A0A6M4JJK7
uniprot id: P45942
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Deletion of the yqcF-yqcG operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Probably interacts with cognate immunity protein YqcF but not with non-cognate immunity proteins. The interaction inhibits the toxic activity of YqcF (Probable).
messages: Secreted
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YqcF does not cause growth arrest. The toxic effect is dependent on the epsA and tapA operons which are required for biofilm formation.
messages: Was originally thought to be an RNase; when the C-terminus (residues 379-531) is expressed in E.coli it has RNase, not DNase activity, and inhibits growth upon expression in E.coli. In vitro RNase activity and in vivo growth inhibition are neutralized by cognate immunity protein YobK, but not by immunity proteins specific to other LXG toxins.

224308:BSU 15500
gene id: 224308:BSU_15500
uniprot id: A0A6M4JMV2
uniprot id: P25995
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.
messages: Homodimer.

224308:BSU 37070
gene id: 224308:BSU_37070
uniprot id: A0A6M3ZH72
uniprot id: Q03223
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Binds 1 zinc ion per subunit. This zinc stabilizes the protein.
messages: Binds the 23S rRNA.
messages: Only accumulates during exponential growth.
messages: Part of the 50S ribosomal subunit during exponential growth.
messages: Part of the 50S ribosomal subunit.
messages: While neither of the L31 paralogs is essential, this protein seems to function as the main L31 protein. Has a lower affinity for 70S ribosomes than the non-zinc-containing paralog L31B (ytiA); is displaced by it to varying extents, even under zinc-replete conditions.

224308:BSU 30500
gene id: 224308:BSU_30500
uniprot id: O34707
names: Function
names: Similarity
messages: Belongs to the large terpene synthase family.
messages: Catalyzes the transformation of a linear C35 prenyl diphosphate chain to form tetraprenyl-beta-curcumene.

224308:BSU 36680
gene id: 224308:BSU_36680
uniprot id: P70963
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31700
gene id: 224308:BSU_31700
uniprot id: P45453
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Interacts directly with the sensor histidine kinase ComP and stimulates its activity.
messages: Part of a major quorum-sensing system that regulates the development of genetic competence (PubMed:8168130, PubMed:11751817, PubMed:16091051, PubMed:12067344). Acts through the activation of the two-component regulatory system ComP/ComA composed of a sensor histidine kinase, ComP, and a response regulator, ComA (PubMed:8168130, PubMed:16091051). Activation of ComA leads to the direct regulation of over 20 genes and expression of over 150 additional genes, including those involved in competence development, appears to be controlled indirectly (PubMed:16091051). Under certain conditions plays a role in sporulation (PubMed:8168130).
messages: Secreted
messages: The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus. In ComX, the sole conserved residue is the modified tryptophan essential for the activity.
messages: Trp-53 is modified by farnesylation, which is essential for activity (PubMed:8168130, PubMed:12067344, PubMed:18323630). Modified by the tryptophan prenyltransferase ComQ before export to the extracellular environment (PubMed:8168130, PubMed:12067344). The type of isoprenyl derivative differs among the different pherotypes and depends on ComX primary sequence (PubMed:12067344, PubMed:14679219).

224308:BSU 12970
gene id: 224308:BSU_12970
uniprot id: O34851
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S66 family.
messages: Cytoplasm
messages: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides.
messages: Repressed by AbrB, a transcription factor that negatively controls biofilm formation.

224308:BSU 40290
gene id: 224308:BSU_40290
uniprot id: O32293
names: Similarity
names: Subunit
messages: Belongs to the acetyltransferase family.
messages: Homodimer.

224308:BSU 27950
gene id: 224308:BSU_27950
uniprot id: P26942
names: Similarity
messages: To M.genitalium MG233.

224308:BSU 23770
gene id: 224308:BSU_23770
uniprot id: A0A6M4JM76
uniprot id: P54555
names: Similarity
messages: Belongs to the serine/threonine dehydratase family. DsdA subfamily.

224308:BSU 18239
gene id: 224308:BSU_18239
uniprot id: C0H419
names: Induction
names: PTM
messages: Can be both biotinylated and lipoylated on Lys-35 upon overexpression in E.coli depending on the growth medium; the nature of the modification in situ in B.subtilis is unknown.
messages: Detected in log-phase B.subtilis cells (at protein level).

224308:BSU 24970
gene id: 224308:BSU_24970
uniprot id: A0A6M3ZE53
uniprot id: P46340
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the binding-protein-dependent transport system YqgGHIJK. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 14330
gene id: 224308:BSU_14330
uniprot id: A0A6M3ZAN6
uniprot id: O31708
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 39350
gene id: 224308:BSU_39350
uniprot id: A0A6M4JQW7
uniprot id: P10944
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PAL/histidase family.
messages: By histidine.
messages: Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.
messages: Cytoplasm

224308:BSU 08810
gene id: 224308:BSU_08810
uniprot id: P21344
names: Function
names: Similarity
messages: Regulates the expression of extracellular-protein genes of Bacillus subtilis.
messages: To B.natto SenN.

224308:BSU 40840
gene id: 224308:BSU_40840
uniprot id: A0A6M4JRC5
uniprot id: P37514
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 12780
gene id: 224308:BSU_12780
uniprot id: P39797
names: Function
names: Similarity
messages: Not known; does not seem to be involved in host cell lysis.
messages: To B.subtilis YqxG/YqdC.

224308:BSU 03651
gene id: 224308:BSU_03651
uniprot id: C0H3U9
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
messages: A triple bsdB-bsdC-bsdD deletion mutant no longer converts vanillin to guaiacol, the conversion stops at vanillic acid.
messages: Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives under both aerobic and anaerobic conditions, however the precise biochemical function of BsdD in metabolism of phenolic acid is unknown.
messages: It is not known, if phenolic acid decarboxylase forms a complex composed of BsdB, BsdC and BsdD. The term subunit is often used in reference to the operon, however there is no experimental evidence to prove the existence of the complex.
messages: Up-regulated by salicylate via the transcriptional regulator BsdA.

224308:BSU 27350
gene id: 224308:BSU_27350
uniprot id: O32035
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the peptidase U32 family.
messages: Deletion mutant lacking both trhP1 and trhP2 shows reduced 5-methoxyuridine (mo5U) formation in tRNAs.
messages: Involved in prephenate-dependent formation of 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs, the first step in 5-methoxyuridine (mo5U) biosynthesis.

224308:BSU 14290
gene id: 224308:BSU_14290
uniprot id: O31704
names: Function
names: Similarity
messages: Belongs to the MobB family.
messages: GTP-binding protein that is not required for the biosynthesis of Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor, and not necessary for the formation of active molybdoenzymes using this form of molybdenum cofactor. May act as an adapter protein to achieve the efficient biosynthesis and utilization of MGD. Displays a weak intrinsic GTPase activity (By similarity).

224308:BSU 04410
gene id: 224308:BSU_04410
uniprot id: P96597
names: Similarity
messages: Belongs to the SchB/CurC family.

224308:BSU 37040
gene id: 224308:BSU_37040
uniprot id: A0A6M4JQ31
uniprot id: P45869
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the auxin efflux carrier (TC 2.A.69) family.
messages: Cell membrane
messages: Membrane

224308:BSU 15400
gene id: 224308:BSU_15400
uniprot id: A0A6M4JMV1
uniprot id: O31729
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YggT family.
messages: Cell membrane

224308:BSU 10009
gene id: 224308:BSU_10009
uniprot id: C0H3Y3
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30530
gene id: 224308:BSU_30530
uniprot id: A0A6M4JME7
uniprot id: O34618
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 39300
gene id: 224308:BSU_39300
uniprot id: A0A6M3ZHU5
uniprot id: P42296
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: In the N-terminal section; belongs to the LXG family.
messages: Probably interacts with cognate immunity protein YxxD but not with non-cognate immunity proteins. The interaction inhibits the toxic activity of YxxD (Probable).
messages: Secreted
messages: Toxic component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. Overexpression alone in situ causes growth arrest but not cell lysis, a large decrease in chromosomal DNA content and the production of anucleate cells. No effect is seen on rRNA. Co-overexpression with cognate immunity protein YxxD does not cause growth arrest. The toxic effect is not dependent on the epsA and tapA operons which are required for biofilm formation.
messages: Was originally thought to be an RNase; when the C-terminus (residues 409-569) is expressed in E.coli it has RNase, not DNase activity, and inhibits growth upon expression in E.coli. In vitro RNase activity and in vivo growth inhibition are neutralized by cognate immunity protein YxxD, but not by immunity proteins specific to other toxins with the LXG domain. Mutation of His-548 to Ala leads to loss of growth inhibition and RNase activity in E.coli.

224308:BSU 24690
gene id: 224308:BSU_24690
uniprot id: A0A6M4JMD0
uniprot id: P25957
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cell surface
messages: Processing of ComGE in competent cells requires ComC.
messages: Required for transformation and DNA binding.

224308:BSU 28430
gene id: 224308:BSU_28430
uniprot id: A0A6M4JNW0
uniprot id: P08066
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
messages: Binds 1 [2Fe-2S] cluster.
messages: Binds 1 [3Fe-4S] cluster.
messages: Binds 1 [4Fe-4S] cluster.
messages: In B.subtilis succinate dehydrogenase forms part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

224308:BSU 25940
gene id: 224308:BSU_25940
uniprot id: P45939
names: Similarity
messages: To B.subtilis XkdW.

224308:BSU 03700
gene id: 224308:BSU_03700
uniprot id: A0A6M4JEX2
uniprot id: P49939
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKA family.
messages: Cell membrane
messages: Involved in the germination response to the combination of glucose, fructose, L-asparagine, and KCl.
messages: Membrane

224308:BSU 30250
gene id: 224308:BSU_30250
uniprot id: O34973
names: Similarity
messages: Belongs to the dienelactone hydrolase family.

224308:BSU 28620
gene id: 224308:BSU_28620
uniprot id: A0A6M3ZJU5
uniprot id: P94541
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RNase HII family. RnhC subfamily.
messages: Cytoplasm
messages: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
messages: Interacts with the RNA polymerase core.
messages: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.

224308:BSU 37080
gene id: 224308:BSU_37080
uniprot id: Q03222
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Rho family.
messages: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.
messages: Homohexamer. The homohexamer assembles into an open ring structure.

224308:BSU 29310
gene id: 224308:BSU_29310
uniprot id: A0A6M4JME6
uniprot id: O34974
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the NtaA/SnaA/DszA monooxygenase family.
messages: Homodimer.
messages: Induced when methionine is the sulfur source, but not by sulfate.
messages: Involved in a cysteine salvage pathway from S-alkylcysteine. Catalyzes the C-S bond cleavage in N-acetyl-S-benzyl-L-cysteine sulfoxide leading to N-acetyl-S-hydroxy-L-cysteine and benzaldehyde. This pathway is likely important in the catabolism of alkylated cysteine generated by proteolysis of alkylated glutathione formed in the detoxification of a wide range of electrophiles. Has much less efficient activity with N-acetyl-S-methyl-L-cysteine sulfoxide as substrate. Cannot use S-alkylated L-cysteine sulfones and ketone analogs as substrates, demonstrating that the sulfoxide is required for activity.
messages: Reduced growth with methionine or methionine sulfoxide as unique source of sulfur.

224308:BSU 15530
gene id: 224308:BSU_15530
uniprot id: A0A6M3ZAT8
uniprot id: P25983
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the PyrK family.
messages: Binds 1 FAD per subunit.
messages: Binds 1 [2Fe-2S] cluster per subunit.
messages: Decreased growth rate in pyrimidine-free medium, extreme derepression of the pyrimidine biosynthetic operon, and decreased, but detectable dihydroorotate dehydrogenase activity.
messages: Heterotetramer of 2 PyrK and 2 PyrD type B subunits.
messages: Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD subunit to the ultimate electron acceptor NAD(+).
messages: Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).

224308:BSU 36570
gene id: 224308:BSU_36570
uniprot id: P71042
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 17120
gene id: 224308:BSU_17120
uniprot id: O34787
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Cytoplasm
messages: In the N-terminal section; belongs to the FabD family.
messages: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductase activity.

224308:BSU 13710
gene id: 224308:BSU_13710
uniprot id: O31674
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 09440
gene id: 224308:BSU_09440
uniprot id: A0A6M4JEQ9
uniprot id: P39119
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the citrate synthase family.
messages: By decoyinine and nutrient depletion.
messages: Citrate synthase is found in nearly all cells capable of oxidative metabolism.
messages: Homodimer.
messages: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect.

224308:BSU 37160
gene id: 224308:BSU_37160
uniprot id: A0A6M3ZHD9
uniprot id: P12464
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the RpoE family.
messages: No visible phenotype; grows as well as wild-type at 25-49 degrees Celsius, no apparent impairment in sporulation or phage SP01 growth.
messages: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling.
messages: Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling. May function in sigma factor switching. It displaces RNA bound to RNA polymerase in a binary complex.
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:6802805, PubMed:18289874, PubMed:21710567). When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
messages: RNAP is composed of a core of 2 alpha, a beta and a beta' subunits. The core is associated with a delta subunit and one of several sigma factors.

224308:BSU 37270
gene id: 224308:BSU_37270
uniprot id: P42176
names: Cofactor
names: Function
names: Subcellular Location Annotation
messages: Binds 1 [3Fe-4S] cluster per subunit.
messages: Binds 3 [4Fe-4S] clusters per subunit.
messages: Cell membrane
messages: The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.

224308:BSU 03610
gene id: 224308:BSU_03610
uniprot id: A0A6M3Z7F6
uniprot id: P42199
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex TcyABC involved in L-cystine import.
messages: The complex is composed of two ATP-binding proteins (TcyC), two transmembrane proteins (TcyB) and a solute-binding protein (TcyA).

224308:BSU 08890
gene id: 224308:BSU_08890
uniprot id: P97029
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32820
gene id: 224308:BSU_32820
uniprot id: A0A6M3ZGE8
uniprot id: O32176
names: Function
names: Induction
names: Similarity
messages: Belongs to the acyl-CoA dehydrogenase family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 04140
gene id: 224308:BSU_04140
uniprot id: A0A6M4JK47
uniprot id: P42971
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: Forespore inner membrane
messages: Forespore outer membrane
messages: Membrane raft
messages: No visible phenotype in growth, sporulation, spore germination, outgrowth, or spore heat resistance (PubMed:8830698). No visible phenotype in the absence of antibiotics; loss of resistance to oxacillin and cephalexin but not penicillin G. It cannot be deleted in the absence of a catalytically inactive copy of penicillin-binding protein 2B (pbpB) (PubMed:28792086).
messages: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis (Probable). Probably required for both cortical and vegetative peptidoglycan synthesis (Probable). Although not usually required for cell division, in the absence of PBP 2B (pbpB) it becomes essential. Confers resistance to oxacillin and cephalexin (PubMed:28792086).
messages: Present in all growth stages and in both inner and outer forespore membranes.
messages: Transcribed during vegetative growth, drops off after entry into stationary phase and the onset of sporulation. Transcription rises again 70-80 minutes after germination.

224308:BSU 37470
gene id: 224308:BSU_37470
uniprot id: P71001
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Phr family.
messages: Contains a predicted signal peptide cleavage site in the N-terminal region, however the propeptide is probably subject to only one processing event, at the N-terminal end of the mature peptide.
messages: Cytoplasm
messages: Deletion of the gene results in decreased expression of genes activated by ComA, including rapA and the srfA operon (PubMed:15968044, PubMed:16816200). It significantly changes the expression of 72 operons (PubMed:16816200).
messages: Interacts with RapF and inhibits its interaction with ComA.
messages: Part of the rapF-phrF operon, which is controlled by ComA (PubMed:15968044). Transcription of phrF is also regulated by the sigma-H factor (PubMed:15968044).
messages: RapF and PhrF pair of proteins acts synergistically with RapC and CSF in the overall regulation of the ComA transcription factor (PubMed:15968044). CSF, PhrF and PhrK stimulate ComA-dependent gene expression to different levels and are all required for full expression of genes activated by ComA (PubMed:16816200).
messages: Secreted
messages: Signaling molecule involved in the regulation of genetic competence development (PubMed:15968044, PubMed:16816200). Secreted during production, but the mature peptide acts intracellularly, indicating that it needs to be imported into the cell to function (By similarity). Stimulates expression of the genes controlled by ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:15968044, PubMed:16816200). Acts by inhibiting RapF, which regulates the activity of ComA (PubMed:15968044, PubMed:16816200, PubMed:22215984, PubMed:23526880).

224308:BSU 23530
gene id: 224308:BSU_23530
uniprot id: A0A6M3ZDV5
uniprot id: P37873
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Component of the MPD complex composed of SpoIIM, SpoIIP and SpoIID.
messages: Expression starts 2 hours post-sporulation.
messages: Required for complete septum migration and engulfment of the forespore compartment during sporulation. Required for stabilizing and recruiting of SpoIIP to the septal membrane.
messages: Results in a block at stage II of sporulation. No fully engulfed forespores. SpoIIP mislocalization from the septal membrane.

224308:BSU 14760
gene id: 224308:BSU_14760
uniprot id: O07630
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31080
gene id: 224308:BSU_31080
uniprot id: P71014
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the BslA/BslB family.
messages: Forms polymers.
messages: Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882).
messages: Overall loss in complex colony architecture and lack of fruiting body-like structures (PubMed:18978066). Disruption of the gene results in the loss of surface repellency and alters the biofilm surface microstructure (PubMed:22571672).
messages: Secreted
messages: Up-regulated by DegU and Spo0A. Repressed by AbrB.
messages: cell wall

224308:BSU 19240
gene id: 224308:BSU_19240
uniprot id: P80872
names: Induction
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 38050
gene id: 224308:BSU_38050
uniprot id: A0A6M4JQE9
uniprot id: P05306
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Acts as both a kinase and a phosphatase on SacT.
messages: Cell membrane
messages: Membrane
messages: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
messages: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in sucrose transport.

224308:BSU 30360
gene id: 224308:BSU_30360
uniprot id: Q795Q5
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 13150
gene id: 224308:BSU_13150
uniprot id: O34777
names: Activity Regulation
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Cys-15 is oxidized by organic peroxides to cysteine sulfenic acid (Cys-SOH). This can react with the alpha-amido of the following residue to form the sulfenamide cross-link. Oxidation or cross-linking results in the loss of DNA-binding activity and the inactivation of repressor function. Both the cysteine sulfenic acid and the sulfenamide cross-link can react with free cysteine or bacillithiol (BSH) to form mixed disulfides. Further reduction of OhrR by free sulfhydryl compounds restores repressor activity.
messages: Cytoplasm
messages: Homodimer.
messages: Inactivated by oxidation of Cys-15 to a sulfenic acid.
messages: Organic peroxide sensor (PubMed:11418552). Represses the expression of the peroxide-inducible gene ohrA by cooperative binding to two inverted repeat elements (PubMed:11418552, PubMed:24313874).

224308:BSU 25080
gene id: 224308:BSU_25080
uniprot id: P54481
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36860
gene id: 224308:BSU_36860
uniprot id: A0A6M4JQ20
uniprot id: P37815
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase C chain family.
messages: Cell membrane
messages: F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane

224308:BSU 14000
gene id: 224308:BSU_14000
uniprot id: A0A6M3ZAH1
uniprot id: P16524
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
messages: Cytoplasm
messages: Essential for murein biosynthesis (PubMed:29280348). Probably catalyzes the conversion of L-2-acetamido-6-oxopimelate to N-acetyl-LL-2,6-diaminopimelate (Probable).
messages: Homodimer.

224308:BSU 35970
gene id: 224308:BSU_35970
uniprot id: P96729
names: Activity Regulation
names: Subcellular Location Annotation
messages: Increases in stationary phase in a strain lacking the WprA protease.
messages: cell wall

224308:BSU 00110
gene id: 224308:BSU_00110
uniprot id: A0A6M3ZBJ9
uniprot id: P37527
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the PdxS/SNZ family.
messages: By superoxide.
messages: Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.
messages: Homohexamer and homododecamer. In the presence of PdxT, forms a dodecamer of heterodimers.
messages: In the presence of PdxT, forms a dodecamer of heterodimers.

224308:BSU 19700
gene id: 224308:BSU_19700
uniprot id: O34895
names: Biotechnology
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the acetyltransferase family.
messages: Deletion of the genomic region encompassing the entire yodT-yodS-yodR-yodQ-yodP-kamA gene cluster has no noticeable effect on growth either in rich or minimal medium, does not affect sporulation, and does not cause osmotic sensitivity or influence the compatible solute pool of this soil bacterium.
messages: In vitro, is able to catalyze the acetylation of beta-lysine to N6-acetyl-beta-lysine, an archaeal osmolyte produced by methanogenic archaea. Its physiological function has not yet been elucidated.
messages: N6-acetyl-beta-lysine is not synthesized by B.subtilis as part of its cellular defense against high salinity.
messages: The use of YodP from B.subtilis for N6-acetyl-beta-lysine synthesis opens the bottleneck for the large-scale production of N6-acetyl-beta-lysine to investigate its properties as a compatible solute.
messages: Up-regulated during sporulation, under the control of the sigma-E transcription factor (SigE).

224308:BSU 24050
gene id: 224308:BSU_24050
uniprot id: A0A6M4JLL1
uniprot id: P37940
names: Function
names: Similarity
names: Subunit
messages: Belongs to the BCKDHA family.
messages: Heterotetramer of two alpha and two beta chains. Directly associated with ODBB in the E1 complex.
messages: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 03370
gene id: 224308:BSU_03370
uniprot id: A0A6M4JID2
uniprot id: P42399
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 13090
gene id: 224308:BSU_13090
uniprot id: A0A6M3Z9W7
uniprot id: P49856
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
messages: Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. YkkC/YkkD subfamily.
messages: Cell membrane
messages: Deletion of both gdnC and gdnD leads to a decrease in the concentration of guanidine required to inhibit bacterial growth.
messages: Membrane
messages: Probably involved in guanidinium transport (PubMed:27989440). In vitro, confers resistance to a broad range of toxic compounds such as cationic dyes, neutral and anionic antimicrobials (PubMed:10735877).
messages: The efflux pump is composed of GdnC and GdnD.
messages: Transcriptionally regulated by guanidine, via a guanidine-sensing riboswitch.

224308:BSU 22180
gene id: 224308:BSU_22180
uniprot id: A0A6M4JN81
uniprot id: P0CI74
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the GpsB family.
messages: By high-salt conditions.
messages: Cytoplasm
messages: Divisome component that associates with the complex late in its assembly, after the Z-ring is formed, and is dependent on DivIC and PBP2B for its recruitment to the divisome. Together with EzrA, is a key component of the system that regulates PBP1 localization during cell cycle progression. Its main role could be the removal of PBP1 from the cell pole after pole maturation is completed. Also contributes to the recruitment of PBP1 to the division complex. Not essential for septum formation.
messages: Forms polymers through the coiled coil domains. Interacts with PBP1, MreC and EzrA.

224308:BSU 01740
gene id: 224308:BSU_01740
uniprot id: A0A6M4JCE0
uniprot id: Q45588
names: Cofactor
names: Domain
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family.
messages: Binds 1 Zn(2+) ion per subunit. Absence of the Zn(2+) (in a residue 1-80 fragment) does not prevent interaction with SigW, nor does it change the overall conformation of RsiW, although a disulfide bond can form between Cys-3 and Cys-37 (PubMed:28319136).
messages: By different stresses causing damage to the cell envelope, such as alkaline shock (PubMed:11454200), salt shock (PubMed:11544224), phage infection and certain antibiotics that affect cell wall biosynthesis (PubMed:12207695, PubMed:15870467).
messages: Cell membrane
messages: Forms a heterodimer with cognate sigma factor SigW, which probably prevents SigW from binding to DNA (PubMed:28319136).
messages: Is processed by successive proteolytic events. First, the extracellular region of RsiW is cleaved by PrsW (site-1 cleavage) in response to cell envelope stresses (PubMed:16816000, PubMed:17020587). In a reconstituted E.coli system PrsW cuts between Ala-168 and Ser-169 followed by trimming by E.coli Tsp; the endogenous extracellular exopeptidase responsible for the event in B.subtilis has not been identified (PubMed:19889088). Next, it undergoes cleavage at an intramembrane site (site-2 cleavage) mediated by RasP (PubMed:15130127). This cleavage uncovers a cryptic proteolytic tag with conserved alanine residues in the transmembrane segment, that is recognized mainly by the ClpXP protease, which completely degrades the protein in the cytoplasm and leads to the induction of the sigma-W-controlled genes (PubMed:16899079).
messages: The anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-W (SigW). Holds SigW, its cognate ECF sigma factor, in an inactive form until released by regulated intramembrane proteolysis (RIP). SigW and RsiW mediate cell response to cell wall stress (PubMed:12207695). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, PrsW) (PubMed:16816000, PubMed:17020587), then within the membrane itself (site-2 protease, S2P, RasP) (PubMed:15130127), while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-W (PubMed:16899079).
messages: The cytoplasmic domain is able to inactivate SigW and the extracellular and transmembrane domains are crucial for sensing and transducing the signal that triggers SigW activation (PubMed:15130127). The N-terminus binds the zinc ion and is followed by a long helix (about residues 40-80) that fits into a hydrophobic surface groove on SigW, probably blocking its ability to interact with the -10 and -35 promoter elements (PubMed:28319136).

224308:BSU 23360
gene id: 224308:BSU_23360
uniprot id: A0A6M3ZDH2
uniprot id: P35137
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cyclophilin-type PPIase family.
messages: Cytoplasm
messages: Inhibited by cyclosporin A (CsA).
messages: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

224308:BSU 03070
gene id: 224308:BSU_03070
uniprot id: A0A6M3Z7C0
uniprot id: P96712
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Confers resistance to puromycin, tosufloxacin and norfloxacin.
messages: Expression is dependent on the growth phase and decreases in the late log phase.
messages: Membrane

224308:BSU 06920
gene id: 224308:BSU_06920
uniprot id: O31513
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 36530
gene id: 224308:BSU_36530
uniprot id: P94571
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.
messages: Belongs to the BcrC/YbjG family.
messages: Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.
messages: Cell membrane
messages: Disruption results in a marked decrease of resistance to bacitracin.
messages: Expression is sigma M, sigma X and sigma I-dependent. Induced by vancomycin and bacitracin, via sigma M. Expression is maximal during early exponential phase and decreases during stationary phase.

224308:BSU 23150
gene id: 224308:BSU_23150
uniprot id: A0A6M4JLF1
uniprot id: P35160
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioredoxin family. ResA subfamily.
messages: Cell membrane
messages: Thiol-disulfide oxidoreductase which is required in disulfide reduction during c-type cytochrome synthesis. May accept reducing equivalents from CcdA, leading to breakage of disulfide bonds in apocytochrome c; following this reduction heme can be covalently attached.
messages: Thiol-disulfide oxidoreductase which is required in disulfide reduction during c-type cytochrome synthesis. May accept reducing equivalents from CcdA, leading to breakage of disulfide bonds in apocytochrome c; following this reduction heme can be covalently attached. Does not play a role in sporulation.

224308:BSU 03500
gene id: 224308:BSU_03500
uniprot id: P80355
names: Function
messages: Required for the development of competence.

224308:BSU 40050
gene id: 224308:BSU_40050
uniprot id: P10585
names: Function
messages: Transcriptional repressor of the gluconate operon (gntRKPZ), which encodes the proteins for gluconate utilization. Represses mRNA synthesis by binding to the gnt operator; the binding is suppressed by gluconate or glucono-delta-lactone.

224308:BSU 07100
gene id: 224308:BSU_07100
uniprot id: P37966
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02350
gene id: 224308:BSU_02350
uniprot id: A0A6M3Z6Z8
uniprot id: P39816
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is repressed by the HTH-type transcriptional regulator GamR.
messages: Membrane
messages: The PTS EIIA type-1 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-1 domain.
messages: The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.
messages: The PTS EIIC type-1 domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system may be involved in glucosamine transport.

224308:BSU 33870
gene id: 224308:BSU_33870
uniprot id: O32246
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 17260
gene id: 224308:BSU_17260
uniprot id: A0A6M3ZEL5
uniprot id: O31788
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S8 family.
messages: Cells lacking this gene show normal growth and sporulation.
messages: Cytoplasm
messages: Displays serine protease activity. Seems to have a broad substrate specificity.
messages: Is completely inhibited by phenylmethanesulphonylfluoride (PMSF) in vitro.
messages: Is expressed during the late stationary phase of growth. Is down-regulated by SinR.

224308:BSU 28210
gene id: 224308:BSU_28210
uniprot id: A0A6M4JMX4
uniprot id: P42425
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity).
messages: Belongs to the peptidase S16 family.
messages: By heat shock.
messages: Cytoplasm
messages: Homohexamer. Organized in a ring with a central cavity (By similarity).
messages: Restricted to the prespore compartment of sporulating cells.

224308:BSU 22690
gene id: 224308:BSU_22690
uniprot id: P19080
names: Function
names: Miscellaneous
names: Subcellular Location Annotation
names: Subunit
messages: Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.
messages: Cytoplasm
messages: Homotrimer.
messages: This enzyme is monofunctional, and its activity is unaffected by the end-product aromatic amino acids.

224308:BSU 09430
gene id: 224308:BSU_09430
uniprot id: A0A6M4JHT6
uniprot id: P39127
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LysR transcriptional regulatory family.
messages: Cytoplasm
messages: Negative regulatory protein for the citA gene for citrate synthase I.

224308:BSU 25710
gene id: 224308:BSU_25710
uniprot id: P54450
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
messages: Expressed during the late sporulation phase.
messages: Expression is GerE and sigma K-dependent.
messages: Secreted

224308:BSU 02630
gene id: 224308:BSU_02630
uniprot id: A0A6M3Z7N0
uniprot id: O31467
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis possesses two minimal, substrate-specific, Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a TatA and a TatC protein. TatA is bifunctional and performs the function of both the TatA and TatB proteins of Gram-negative organisms.
messages: Belongs to the TatA/E family.
messages: Cell membrane
messages: Expressed under conditions of phosphate starvation.
messages: Forms a complex with TatC.
messages: Forms a complex with TatCd. Two types of complexes exist: one composed of TatAd and TatCd, and another composed only of TatAd. Soluble TatAd forms homomultimers bigger than 100 kDa. Soluble TatAd associates in homomultimeric micelle-like particles with a size of about 12 or 25 nm in diameter as well as in elongated particles of 12 nm in diameter.
messages: Membrane-localization and maintenance of TatAd is assisted by TatCd.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. Required for PhoD secretion. The cytosolic fraction of TatAd binds the precursor of PhoD.
messages: cytosol

224308:BSU 15170
gene id: 224308:BSU_15170
uniprot id: A0A6M4JJR6
uniprot id: Q03524
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Asporogeneous; spore development starts but does not develop the spore cortex.
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: Expressed during sporulation; strong expression 60-150 minutes after sporulation onset, falls to background expression by 240 minutes. Found only in mother cells.
messages: Penicillin-binding protein with an unknown catalytic activity. May have a specialized role in the morphogenesis of spore cortex, which is a modified form of peptidoglycan. Spore cortex formation is determined primarily by the mother cell.

224308:BSU 29520
gene id: 224308:BSU_29520
uniprot id: A0A6M4JMF8
uniprot id: O34424
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 06660
gene id: 224308:BSU_06660
uniprot id: A0A6M3Z8R8
uniprot id: O06493
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
messages: Catalyzes the sodium-dependent uptake of extracellular L-proline.
messages: Catalyzes the uptake of extracellular proline under high-osmolarity growth conditions. Essential for the use of proline present in the environment as an osmoprotectant.
messages: Cell membrane
messages: Disruption of the gene eliminates the osmotically stimulated proline transport activity and practically abolishes the ability to use proline as an osmoprotectant.
messages: Induced under high-osmolarity growth conditions. Has two osmoregulated and tightly spaced promoters. The first promoter is sigma A-dependent and the second promoter is controlled by the general stress transcription factor sigma B.
messages: Membrane

224308:BSU 28800
gene id: 224308:BSU_28800
uniprot id: A0A6M4JMB4
uniprot id: P94523
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the arabinose isomerase family.
messages: Binds 1 Mn(2+) ion per subunit.
messages: Catalyzes the conversion of L-arabinose to L-ribulose.
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 22000
gene id: 224308:BSU_22000
uniprot id: Q7WY66
names: Developmental Stage
names: Induction
names: Subcellular Location Annotation
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination.
messages: Expression is sigma G-dependent.
messages: Spore core

224308:BSU 10460
gene id: 224308:BSU_10460
uniprot id: O07557
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32990
gene id: 224308:BSU_32990
uniprot id: A0A6M3ZJ99
uniprot id: P37960
names: Function
names: Induction
names: Similarity
messages: Belongs to the Dps family.
messages: By oxidative stress and by growth in minimal medium lacking iron (Fe(3+)), or one of the divalent cations manganese, copper or cobalt.
messages: Forms highly stable, multimeric protein-DNA complexes which accumulate in stationary-phase cells and protect against oxidative killing.

224308:BSU 15710
gene id: 224308:BSU_15710
uniprot id: A0A6M3ZBA6
uniprot id: P94461
names: Function
names: Similarity
names: Subunit
messages: Belongs to the helicase family. PriA subfamily.
messages: Component of the primosome.
messages: Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.

224308:BSU 03580
gene id: 224308:BSU_03580
uniprot id: O34927
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36380
gene id: 224308:BSU_36380
uniprot id: P94583
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Rap family.
messages: Cytoplasm

224308:BSU 17780
gene id: 224308:BSU_17780
uniprot id: O31811
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 16110
gene id: 224308:BSU_16110
uniprot id: A0A6M4JMY4
uniprot id: P39813
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DprA/Smf family.
messages: Cytoplasm
messages: Expression activated by ComK (PubMed:11918817, PubMed:11948146).
messages: Interacts with RecA (PubMed:17803906).
messages: Preferentially expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:17630974).
messages: Protein that helps load RecA onto ssDNA during transformation (PubMed:17803906, PubMed:25138221). Binds cooperatively to circular ssDNA, is able to bridge different segments of DNA (PubMed:17803906). Favors the loading of RecA onto SsbA- or SsbB-coated ssDNA and formation of RecA-DNA filaments (PubMed:25138221). RecA-ATP cannot catalyze homologous DNA strand exchange; SsbA and DprA activate strand exchange by RecA-ATP (PubMed:25138221).
messages: Transformation efficiency drops 50- to 100-fold (PubMed:11918817, PubMed:11948146).

224308:BSU 40810
gene id: 224308:BSU_40810
uniprot id: A0A6M4JR66
uniprot id: P37511
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 16740
gene id: 224308:BSU_16740
uniprot id: A0A6M4JIF1
uniprot id: Q04810
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: Dipicolinate synthase likely consists of DpaA and DpaB, since both proteins are required for DPA synthesis.
messages: Disruption of this gene has no effect on vegetative growth but causes a sporulation defect, characterized by very low sporulation frequencies and heat-sensitive spores devoid of DPA, which can be cured by supplementation with dipicolinate.
messages: Is not expressed during vegetative growth; is expressed at stage 5 of sporulation specifically in the mother cell compartment, under the control of the sigma-K factor. Is repressed by GerE.
messages: Together with DpaA, catalyzes the conversion of dihydrodipicolinate to dipicolinate (DPA), which constitutes up to 10% of the dry weight of the spore.
messages: Together with DpaA, catalyzes the conversion of dihydrodipicolinate to dipicolinate (DPA).

224308:BSU 02430
gene id: 224308:BSU_02430
uniprot id: A0A6M3Z7H7
uniprot id: O31465
names: Similarity
names: Subunit
messages: Belongs to the glutaminase family.
messages: Homotetramer.

224308:BSU 14630
gene id: 224308:BSU_14630
uniprot id: A0A6M3ZAC3
uniprot id: P21885
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Orn/Lys/Arg decarboxylase class-I family.
messages: Catalyzes the formation of agmatine from arginine.
messages: Cytoplasm
messages: Was originally (Ref.1) thought to be a lysine decarboxylase and was consequently named cad.

224308:BSU 17230
gene id: 224308:BSU_17230
uniprot id: A0A6M3ZB16
uniprot id: O31785
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome P450 family.
messages: Cell membrane
messages: Involved in the metabolism of the antibiotic polyketide bacillaene which is involved in secondary metabolism. The substrate is dihydrobacillaene.

224308:BSU 32430
gene id: 224308:BSU_32430
uniprot id: O32139
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
messages: Cell membrane
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced by TnrA during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or uric acid are added during limiting-nitrogen conditions.
messages: Uptake of uric acid.

224308:BSU 17270
gene id: 224308:BSU_17270
uniprot id: O31789
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0714 family.
messages: Cell membrane
messages: Induced by mitomycin C (MMC) and UV, and this requires RecA. Also induced by hydrogen prexoxide.

224308:BSU 22830
gene id: 224308:BSU_22830
uniprot id: A0A6M4JKF2
uniprot id: P46919
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
messages: Cells lacking this gene are auxotrophic for glycerol.
messages: Cytoplasm
messages: Involved in the biosynthesis of the sn-glycerol 3-phosphate required for phospholipid synthesis.
messages: Slightly inhibited by sn-glycerol 3-phosphate.

224308:BSU 23160
gene id: 224308:BSU_23160
uniprot id: A0A6M3ZDJ8
uniprot id: P35159
names: Function
names: Similarity
messages: Belongs to the pseudouridine synthase RsuA family.
messages: Responsible for synthesis of pseudouridine from uracil-2633 in 23S ribosomal RNA.

224308:BSU 03870
gene id: 224308:BSU_03870
uniprot id: P94425
names: Function
names: Induction
names: Similarity
messages: Belongs to the LsrG family.
messages: Putative monooxygenase that may contribute to the degradation of aromatic compounds.
messages: Strongly induced by stress due to exposure to 6-brom-2-vinyl-chroman-4-on (chromanon) and less strongly induced after exposure to 2-methylhydroquinone (2-MHQ) or catechol stress.

224308:BSU 01550
gene id: 224308:BSU_01550
uniprot id: P16450
names: Developmental Stage
names: Function
names: Subcellular Location Annotation
messages: Active between T2 and T6 of sporulation.
messages: Cell membrane
messages: May be involved in fructose recognition during germination.

224308:BSU 18849
gene id: 224308:BSU_18849
uniprot id: A0A6M3ZBU0
uniprot id: C0H423
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 31720
gene id: 224308:BSU_31720
uniprot id: Q99039
names: Function
names: Similarity
messages: Belongs to the DegQ family.
messages: Stimulates the phosphotransfer from phospho-DegS to DegU. Affects protease and levansucrose production.

224308:BSU 10330
gene id: 224308:BSU_10330
uniprot id: C0SP94
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Interacts with FloT.
messages: Membrane raft
messages: Transcriptionally regulated by fur; repressed by iron.

224308:BSU 26700
gene id: 224308:BSU_26700
uniprot id: O07923
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AzlD/HI_1737/HP1330 family.
messages: Cell membrane
messages: Involved in branched-chain amino acid transport.

224308:BSU 06890
gene id: 224308:BSU_06890
uniprot id: Q45536
names: Function
messages: The cotJ operon proteins affect spore coat composition. They are either required for the normal formation of the inner layers of the coat or are themselves structural components of the coat.

224308:BSU 38520
gene id: 224308:BSU_38520
uniprot id: A0A6M4JQI6
uniprot id: P39579
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-DCP.
messages: Belongs to the DltC family.
messages: Carrier protein involved in the D-alanylation of lipoteichoic acid (LTA). The loading of thioester-linked D-alanine onto DltC is catalyzed by D-alanine--D-alanyl carrier protein ligase DltA. The DltC-carried D-alanyl group is further transferred to cell membrane phosphatidylglycerol (PG) by forming an ester bond, probably catalyzed by DltD. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
messages: Cytoplasm

224308:BSU 21710
gene id: 224308:BSU_21710
uniprot id: P54181
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
messages: Cell membrane

224308:BSU 07520
gene id: 224308:BSU_07520
uniprot id: O34348
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Part of the ABC transporter complex YfmCDEF involved in citrate-dependent Fe(3+) import. Binds citrate-dependent Fe(3+) and delivers it to the surface of YfmDE (Probable).
messages: The complex is composed of one ATP-binding protein (YfmF), two transmembrane proteins (YfmD and YfmE) and a solute-binding protein (YfmC).
messages: Transcriptionally regulated by fur.

224308:BSU 09460
gene id: 224308:BSU_09460
uniprot id: A0A6M3Z8W7
uniprot id: O07576
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Branched-chain amino acid transport system which is involved in the uptake of isoleucine, valine and probably leucine (PubMed:25645558). Can also transport threonine, and is active as a minor serine permease (PubMed:25645558, PubMed:32743959). May be an amino acid permease of rather broad specificity, because several amino acids, albeit at 100-fold excess, are able to prevent isoleucine uptake (PubMed:25645558). Probably does not transport methionine (PubMed:25645558). Together with BraB and BrnQ, plays an important role in the activation of CodY, a branched-chain amino acid-responsive transcriptional regulator that controls the expression of several dozen transcription units in B.subtilis (PubMed:25645558).
messages: Cell membrane
messages: Expression is repressed by the transcriptional regulator CodY (PubMed:12618455, PubMed:21097623). Contains two CodY-binding sites in the bcaP regulatory region, which both contribute to repression in vivo and do so independently of each other (PubMed:21097623). A single CodY-binding site is apparently sufficient for substantial CodY-dependent regulation, but both upstream and downstram CodY-binding regions are required for maximal repression of bcaP (PubMed:21097623). Efficient bcaP repression by CodY requires the simultaneous presence of isoleucine, leucine and valine, and other amino acids in the growth medium (PubMed:21097623).
messages: Isoleucine uptake is efficiently reduced in the presence of 100-fold excess valine, leucine, alanine, threonine, serine, cysteine, asparagine, and a nonproteinaceous amino acid 4-azaleucine.
messages: Membrane
messages: Mutant is still able to take up isoleucine, leucine and valine (PubMed:25645558). Deletion of the gene confers a weak resistance to growth inhibition by serine (PubMed:32743959). The deletion of the three permease-encoding genes aimA (ybeC), ybxG and bcaP results in an unprecedented resistance to serine up to 100 mM (PubMed:32743959).

224308:BSU 28990
gene id: 224308:BSU_28990
uniprot id: P07908
names: Function
names: Miscellaneous
names: Subunit
messages: Interacts with CcrZ.
messages: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110.
messages: The two mutants dna-1 (dnaBI) and dnaB-19 (dnaBII) show different characteristics for replication and membrane binding of plasmid pUB110. DnaBI is essential for both chromosome and pUB110 replication, whereas dnaBII is necessary only for chromosome replication.

224308:BSU 22960
gene id: 224308:BSU_22960
uniprot id: A0A6M3ZDW6
uniprot id: P50735
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
messages: Constitutively expressed.
messages: GudB seems to be intrinsically inactive, however spontaneous mutations removing a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family (called gutB1). This 3 amino acid insertion presumably causes severe destabilization of the fold of the protein, leading to an inactive enzyme that is very quickly degraded. The cryptic GudB serves as a buffer that may compensate for mutations in the rocG gene and that can also be decryptified for the utilization of glutamate as a single carbon source in the absence of arginine. It is unable to synthesize glutamate.
messages: Homohexamer.
messages: No visible phenotype. Cells lacking this gene show no growth defect; a double rocG-gudB disruption has the same phenotype as a single rocG disruption.

224308:BSU 33520
gene id: 224308:BSU_33520
uniprot id: O32222
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CsoR family.
messages: Can also bind other divalent metal ions (zinc, cobalt and nickel), but these metals are poor regulators of DNA binding.
messages: Cells lacking this gene display a slightly enhanced ability to grow into medium containing high levels of copper.
messages: Copper-sensitive repressor that has a key role in copper homeostasis. Negatively regulates expression of the copZA operon and of ycnJ. In the absence of copper ions, binds with high affinity to the copZA promoter and represses the transcription. In the presence of copper ions, CsoR binds Cu(1+), which significantly decreases its DNA binding affinity and leads to the transcription of the genes.
messages: Cytoplasm
messages: Homotetramer. Binds DNA with a stoichiometry of 2 tetramers per DNA.

224308:BSU 18420
gene id: 224308:BSU_18420
uniprot id: A0A6M4JMC9
uniprot id: O34685
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LysR transcriptional regulatory family.
messages: Cytoplasm
messages: Maximally expressed at stationary phase.
messages: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase.

224308:BSU 08700
gene id: 224308:BSU_08700
uniprot id: P71083
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0421 family.
messages: Cell membrane

224308:BSU 11270
gene id: 224308:BSU_11270
uniprot id: O34713
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 20340
gene id: 224308:BSU_20340
uniprot id: O31902
names: Function
names: Similarity
messages: Belongs to the DNA polymerase type-C family.
messages: Probable DNA polymerase.

224308:BSU 03550
gene id: 224308:BSU_03550
uniprot id: A0A6M3Z7X6
uniprot id: Q08794
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 09660
gene id: 224308:BSU_09660
uniprot id: O07596
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide deacetylase family.
messages: Cell membrane

224308:BSU 24570
gene id: 224308:BSU_24570
uniprot id: A0A6M3ZDU5
uniprot id: P54378
names: Function
names: Similarity
names: Subunit
messages: Belongs to the GcvT family.
messages: The glycine cleavage system catalyzes the degradation of glycine.
messages: The glycine cleavage system is composed of four proteins: P, T, L and H.

224308:BSU 27890
gene id: 224308:BSU_27890
uniprot id: P39667
names: Caution
names: Disruption Phenotype
names: Function
names: Subunit
messages: Homodimer.
messages: In the presence of nicotinic acid represses transcription of the nadBCA and nifS-nadR operons. Also binds to DNA upstream of the niaP gene, probably regulating it as well. May bind nicotinic acid.
messages: It is uncertain whether Met-1 or Met-8 is the initiator.
messages: No visible phenotype when grown in the absence of nicotinic acid.

224308:BSU 35340
gene id: 224308:BSU_35340
uniprot id: A0A6M3ZGG5
uniprot id: P39738
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum
messages: Belongs to the FliD family.
messages: Homopentamer.
messages: Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.
messages: Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end (By similarity).
messages: Secreted

224308:BSU 17470
gene id: 224308:BSU_17470
uniprot id: P31844
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 29350
gene id: 224308:BSU_29350
uniprot id: A0A6M3ZFF4
uniprot id: O34931
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable). Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.
messages: The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).

224308:BSU 27910
gene id: 224308:BSU_27910
uniprot id: A0A6M4JLK5
uniprot id: P21204
names: Similarity
messages: Belongs to the UPF0735 family.

224308:BSU 37890
gene id: 224308:BSU_37890
uniprot id: A0A6M3ZHK1
uniprot id: P39623
names: Similarity
messages: Belongs to the DegT/DnrJ/EryC1 family.

224308:BSU 15610
gene id: 224308:BSU_15610
uniprot id: A0A6M4JLP6
uniprot id: O34744
names: Function
names: Induction
names: Similarity
messages: Belongs to the precorrin methyltransferase family.
messages: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and siroheme.
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 08450
gene id: 224308:BSU_08450
uniprot id: A0A6M3Z902
uniprot id: O31568
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Induced by iron starvation, repressed by fur.
messages: Membrane
messages: Part of the ABC transporter complex YfiYZ/YfhA/YusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen.
messages: Strains lacking this gene show a reduction in growth stimulation by the iron-hydroxamate siderophores schizokinen and arthrobactin compared to wild-type.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (YfiZ and YfhA) and a solute-binding protein (YfiY).

224308:BSU 38980
gene id: 224308:BSU_38980
uniprot id: A0A6M3ZHS1
uniprot id: P42316
names: Similarity
names: Subunit
messages: Belongs to the 3-oxoacid CoA-transferase subunit B family.
messages: Heterodimer of a subunit A and a subunit B.

224308:BSU 40190
gene id: 224308:BSU_40190
uniprot id: A0A6M4JR02
uniprot id: Q45597
names: Activity Regulation
names: Disruption Phenotype
names: Induction
names: Similarity
messages: Activated by phosphoenolpyruvate (PEP) and by high concentrations of monovalent cations, NH(4)(+) being most effective. Is inhibited by two types of compounds which apparently act on the enzyme at different sites. The first group consists of nucleoside monophosphates (most effective are AMP and dAMP), phosphorylated coenzymes, and denatured DNA and RNA, whose inhibition is competed by PEP. The second group, whose inhibition is not competed by PEP, consists of highly phosphorylated nucleotides (pppGpp and pppApp are most effective), nucleoside di- and triphosphates, and PPi.
messages: Belongs to the FBPase class 3 family.
messages: Constitutively expressed. Expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis.
messages: Disruption of fbp alone (or ywjI alone) does not affect growth on various carbon sources. But the ywjI fbp double mutant is unable to grow with carbon sources demanding FBPase activity, i.e. glycerol, malate, and a mixture of succinate and glutamate.

224308:BSU 06980
gene id: 224308:BSU_06980
uniprot id: A0A6M3Z8P4
uniprot id: O31519
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane (By similarity).

224308:BSU 27200
gene id: 224308:BSU_27200
uniprot id: A0A6M4JM87
uniprot id: O05399
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FNT transporter (TC 2.A.44) family.
messages: Cell membrane
messages: Membrane

224308:BSU 38670
gene id: 224308:BSU_38670
uniprot id: A0A6M3ZK98
uniprot id: P94373
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is sigma Y-dependent. Induced upon nitrogen starvation.
messages: Membrane
messages: Together with YxlD is important for negative regulation of sigma Y activity.

224308:BSU 27440
gene id: 224308:BSU_27440
uniprot id: A0A6M4JMT8
uniprot id: O34563
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex GlnHMPQ involved in glutamine transport.
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: The complex is composed of two ATP-binding proteins (GlnQ), two transmembrane proteins (GlnM and GlnP) and a solute-binding protein (GlnH).

224308:BSU 40000
gene id: 224308:BSU_40000
uniprot id: A0A6M3ZI84
uniprot id: O32291
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 28540
gene id: 224308:BSU_28540
uniprot id: A0A6M4JNW2
uniprot id: P94549
names: Function
names: Induction
names: Similarity
messages: Belongs to the enoyl-CoA hydratase/isomerase family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 36900
gene id: 224308:BSU_36900
uniprot id: A0A6M4JQ13
uniprot id: P39148
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SHMT family.
messages: Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
messages: Cytoplasm
messages: Folds into two domains, a large PLP-binding domain (residues 63-276) and a small C-terminal domain.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 30170
gene id: 224308:BSU_30170
uniprot id: A0A6M4JRP5
uniprot id: P53561
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in pectin degradation (PubMed:29240795). Part of the ABC transporter complex YtcQP-YteP involved in the uptake of polygalacturonan and rhamnogalacturonan type I (PubMed:29240795). Responsible for the translocation of the substrate across the membrane (Probable).
messages: Membrane
messages: The complex is probably composed of two ATP-binding proteins (MsmX), two transmembrane proteins (YtcP and YteP) and a solute-binding protein (YtcQ).

224308:BSU 06150
gene id: 224308:BSU_06150
uniprot id: A0A6M3ZB92
uniprot id: Q06004
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the zinc-containing alcohol dehydrogenase family.
messages: Binds 1 Zn(2+) ion per subunit.
messages: Homotetramer.
messages: Polyol dehydrogenase that catalyzes the NAD(+)-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), xylitol and L-iditol as substrates, leading to the C2-oxidized products D-fructose, D-xylulose and L-sorbose, respectively.

224308:BSU 28690
gene id: 224308:BSU_28690
uniprot id: A0A6M3ZI66
uniprot id: P94534
names: Cofactor
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Binds 2 [4Fe-4S] clusters.
messages: Cell membrane
messages: Component of a complex that catalyzes the oxidation of glycolate to glyoxylate.
messages: Component of a complex that catalyzes the oxidation of glycolate to glyoxylate. Is also able to oxidize D-lactate ((R)-lactate). Does not link directly to O(2), and 2,6-dichloroindophenol (DCIP) and phenazine methosulfate (PMS) can act as artificial electron acceptors in vitro, but the physiological molecule that functions as primary electron acceptor during glycolate oxidation is unknown.
messages: The glycolate oxidase likely consists of several subunits including GlcD and GlcF.

224308:BSU 35170
gene id: 224308:BSU_35170
uniprot id: A0A6M4JPL4
uniprot id: P37954
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the UvrB family.
messages: Cytoplasm
messages: Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.
messages: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.
messages: The beta-hairpin motif is involved in DNA binding.

224308:BSU 05060
gene id: 224308:BSU_05060
uniprot id: P96653
names: Function
messages: Negative regulation of glyA transcription and kinB-dependent sporulation.

224308:BSU 09600
gene id: 224308:BSU_09600
uniprot id: A0A6M3Z8Y3
uniprot id: O07590
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.
messages: Cell membrane
messages: Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.
messages: Membrane
messages: Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.

224308:BSU 37460
gene id: 224308:BSU_37460
uniprot id: P71002
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Rap family.
messages: Contains a small N-terminal 3-helix bundle domain and a large C-terminal TPR domain, connected by a linker region (PubMed:22215984, PubMed:23526880). The 3-helix bundle domain and the linker region form the ComA binding surface (PubMed:22215984, PubMed:23526880). A RapF surface mimics DNA to block ComA binding to its target promoters (PubMed:22215984). Interacts with PhrF via the TPR domain (PubMed:23526880).
messages: Cytoplasm
messages: Deletion of the gene has no detectable effect on expression of the srfA operon.
messages: Inhibited by PhrF, which prevents RapF-ComA interaction (PubMed:15968044, PubMed:16816200, PubMed:22215984, PubMed:23526880). Interaction with PhrF induces a conformational change in RapF, which is propagated to the ComA binding site and causes the dissociation of ComA from RapF (PubMed:22215984, PubMed:23526880).
messages: Involved in the regulation of genetic competence development (PubMed:15968044, PubMed:16816200). Inhibits the activity of ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:15968044, PubMed:16816200, PubMed:22215984, PubMed:23526880). Acts by binding to ComA, leading to the inhibition of its DNA-binding activity (PubMed:15968044, PubMed:22215984, PubMed:23526880). May also affect transcription independently of ComA (PubMed:16816200).
messages: Monomer (PubMed:22215984). Is monomeric either alone or in complex with PhrF (PubMed:22215984). Interacts specifically with the C-terminal DNA-binding domain of ComA (PubMed:15968044, PubMed:22215984). Interacts with PhrF (PubMed:15968044, PubMed:22215984, PubMed:23526880).
messages: Part of the rapF-phrF operon, which is controlled by ComA.
messages: RapF and PhrF pair of proteins acts synergistically with RapC and CSF in the overall regulation of the ComA transcription factor.

224308:BSU 38940
gene id: 224308:BSU_38940
uniprot id: A0A6M3ZHV9
uniprot id: P94352
names: Similarity
messages: Belongs to the LOR family.

224308:BSU 21460
gene id: 224308:BSU_21460
uniprot id: P68569
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thioredoxin family.
messages: Secreted
messages: Unknown; dispensable for production of the lantibiotic sublancin 168 and for competence for DNA uptake.

224308:BSU 32690
gene id: 224308:BSU_32690
uniprot id: A0A6M4JNX2
uniprot id: O32164
names: Activity Regulation
names: Function
names: Similarity
names: Subunit
messages: A Cys to Ala mutation in SufU (Cys-41-Ala) has been described to be a competivie inhibitor of SufS activity and a non-competitive inhibitor (PubMed:21236255, PubMed:24321018).
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.
messages: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine.
messages: Homodimer (PubMed:27382962). Interacts with SufU; this interaction induces an opening of the active site pocket of SufS (PubMed:27382962). Interacts with frataxin/Fra (PubMed:27382962).
messages: Type II cysteine desulfurase that acts as the initial step in the SUF-like Fe-S cluster assembly pathway. Catalyzes the removal of elemental sulfur atoms from L-cysteine by using the cofactor pyridoxal 5'-phosphate (PLP), resulting in the production of L-alanine and persulfide (PubMed:31587510). Activity is stimulated by SufU, which acts as a sulfurtransferase that receives sulfur from SufS via a zinc-ligand swapping mechanism and transfers it to SufB (PubMed:29235855, PubMed:27382962).

224308:BSU 20730
gene id: 224308:BSU_20730
uniprot id: O34401
names: Subunit
messages: Homodimer.

224308:BSU 16360
gene id: 224308:BSU_16360
uniprot id: A0A6M4JIP6
uniprot id: P35535
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliQ/MopD/SpaQ family.
messages: Cell membrane
messages: Membrane
messages: Role in flagellar biosynthesis.

224308:BSU 37960
gene id: 224308:BSU_37960
uniprot id: A0A6M4JQE7
uniprot id: P39616
names: Similarity
messages: Belongs to the aldehyde dehydrogenase family.

224308:BSU 06840
gene id: 224308:BSU_06840
uniprot id: A0A6M3ZBG7
uniprot id: O31509
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TACO1 family. YeeN subfamily.
messages: Cytoplasm

224308:BSU 23220
gene id: 224308:BSU_23220
uniprot id: A0A6M3ZIU5
uniprot id: P35154
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ScpA family.
messages: Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.
messages: Cytoplasm
messages: Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.

224308:BSU 34380
gene id: 224308:BSU_34380
uniprot id: P71049
names: Function
names: Subunit
messages: Component of the SlrR/SlrA complex.
messages: Represses sigma(D)-dependent flagellar genes and activate the eps and yqxM operons. Repressor activity is regulated by SlrA. Controls the initiation of biofilm formation.

224308:BSU 02170
gene id: 224308:BSU_02170
uniprot id: A0A6M3ZIS0
uniprot id: O31444
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 30780
gene id: 224308:BSU_30780
uniprot id: A0A6M3ZFL1
uniprot id: O34514
names: Function
names: Similarity
messages: Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 2 subfamily.
messages: Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB) (PubMed:10194342). Does not show detectable N-acylamino acid racemase (NAAAR) activity with N-acetyl-S-methionine as substrate (PubMed:10194342).
messages: Converts 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) to 2-succinylbenzoate (OSB).

224308:BSU 29010
gene id: 224308:BSU_29010
uniprot id: A0A6M3ZI96
uniprot id: O34426
names: Cofactor
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.
messages: Binds 1 pyruvoyl group covalently per subunit.
messages: Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.
messages: Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.
messages: Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
messages: Proteome analysis shows that a mutant overexpresses SAM synthase by comparison to wild-type.

224308:BSU 06710
gene id: 224308:BSU_06710
uniprot id: O31501
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: A SwrC-deficient strain is susceptible to acriflavine and ethidium bromide, and showed severer growth inhibition as surfactin concentration increased up to 100ug/ml.
messages: Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.
messages: Not induced by surfactin.
messages: Required for self-resistance to surfactin, an antimicrobial lipopeptide surfactant produced by B.subtilis. Also required for swarming motility.

224308:BSU 03310
gene id: 224308:BSU_03310
uniprot id: P42434
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.
messages: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
messages: Positively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 14130
gene id: 224308:BSU_14130
uniprot id: O31698
names: Activity Regulation
names: Domain
names: Function
names: Subunit
messages: Binds c-di-AMP (PubMed:31061098, PubMed:33619274, PubMed:35130724, PubMed:35714772). Binding of c-di-AMP to DarB inhibits the interaction with RelA and PYC (PubMed:33619274, PubMed:35130724).
messages: Homodimer (PubMed:33619274, PubMed:35714772). Forms a homodimer with a parallel, head-to-head assembly of the monomers (PubMed:35714772). Under conditions of potassium starvation and corresponding low c-di-AMP levels, apo-DarB specifically interacts with the N-terminal region of the RelA (PubMed:33619274). Under the same conditions, apo-DarB also specifically interacts with the C-terminal part of the pyruvate carboxylase (PubMed:35130724).
messages: Involved in the c-di-AMP-dependent regulation of the bacterial stringent response (PubMed:33619274, PubMed:35130724). Modulates the activities of at least two enzymes under conditions of potassium limitation (PubMed:33619274, PubMed:35130724). Apo-DarB regulates the activity of the GTP pyrophosphokinase RelA by interacting directly with RelA, leading to stimulation of (p)ppGpp synthesis and induction of the stringent response (PubMed:33619274). Apo-DarB also regulates pyruvate carboxylase (PYC) at two levels: directly at the protein level by binding to the enzyme and stimulating the synthesis of oxaloacetate and indirectly, by interaction with RelA, which leads to activation of the stringent response and to the increased expression of the pycA gene (PubMed:35130724). Stimulation of these enzymes by DarB is prevented in the presence of cyclic di-AMP (c-di-AMP) (PubMed:33619274, PubMed:35130724).
messages: No conformational changes occur upon c-di-AMP binding.

224308:BSU 41060
gene id: 224308:BSU_41060
uniprot id: A0A6M4JRB3
uniprot id: P05647
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL34 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 25630
gene id: 224308:BSU_25630
uniprot id: P54456
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Ap4A hydrolase YqeK family.
messages: Homodimer.
messages: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to yield ADP.
messages: Mutant shows increased levels of Ap4G, Ap4U, Ap3A and Ap4A (PubMed:32152217). Cells form pellicles that are initially flat and thin. The pellicles finally become thicker, their flat surfaces are broken by a number of broad pits, the inner surface of which are covered with fruiting bodies. The colonies also show a defect in gross morphology, in that their central region is flat ang glossy (PubMed:15175311).

224308:BSU 27300
gene id: 224308:BSU_27300
uniprot id: O32031
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 23050
gene id: 224308:BSU_23050
uniprot id: A0A6M3ZDI8
uniprot id: P50726
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Abolishes riboflavin uptake, cell growth less inhibited by roseoflavin.
messages: Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.
messages: Cell membrane
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of a membrane-embedded substrate-binding protein (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component). May be able to interact with more than 1 S component at a time (By similarity).
messages: Induced by riboflavin deficiency.
messages: Inhibited by excess of riboflavin or FMN. Also inhibited by protonophores such as CCCP and FCCP or in the absence of glucose.
messages: Mediates uptake of riboflavin and roseoflavin, a toxic riboflavin analog; may also transport FMN. Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (By similarity).
messages: Membrane
messages: Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex.

224308:BSU 37750
gene id: 224308:BSU_37750
uniprot id: A0A6M4JQC6
uniprot id: P39637
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 29210
gene id: 224308:BSU_29210
uniprot id: A0A6M3ZF41
uniprot id: C0SP93
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).
messages: Belongs to the AccD/PCCB family.
messages: Binds 1 zinc ion per subunit.
messages: Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.
messages: Cytoplasm
messages: Inhibited by pyrrolidine dione antibiotics moiramide B (CPD1) and CPD2.

224308:BSU 10050
gene id: 224308:BSU_10050
uniprot id: P55340
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells form colonies in which certain architectural features are absent or less prominent than those observed in wild-type colonies. The Ecs activity could be important for the development of communities.
messages: Presumed to form part of an ABC-transporter, it may form a transport channel.

224308:BSU 08280
gene id: 224308:BSU_08280
uniprot id: P94437
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.

224308:BSU 36880
gene id: 224308:BSU_36880
uniprot id: P37816
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: A possible function for this protein is to guide the assembly of the membrane sector of the ATPase enzyme complex.
messages: Belongs to the bacterial AtpI family.
messages: Cell membrane

224308:BSU 40529
gene id: 224308:BSU_40529
uniprot id: C0H3U2
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 28110
gene id: 224308:BSU_28110
uniprot id: P37963
names: Developmental Stage
names: Function
messages: Expression is initiated around the second hour of sporulation and continues throughout development. May be expressed predominantly in the mother cell.
messages: Required for assembly of a normal spore coat. May be a component of the innermost layer of the spore coat that aids in its adherence to the prespore.

224308:BSU 10730
gene id: 224308:BSU_10730
uniprot id: O06722
names: Function
names: Induction
names: Similarity
messages: Aspartyl-phosphate phosphatase which specifically dephosphorylates the sporulation transcription factor Spo0A-P and negatively regulates the sporulation initiation pathway in order to control the proper timing of sporulation.
messages: Belongs to the spo0E family.
messages: During exponential phase and in conditions antithetical to sporulation.

224308:BSU 40021
gene id: 224308:BSU_40021
uniprot id: A0A6M3ZI22
uniprot id: C0H3T7
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 36180
gene id: 224308:BSU_36180
uniprot id: P96723
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cytoplasm
messages: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon has no visible growth phenotype, however it is out-competed by wild-type cells.
messages: Expressed on rich and minimal solid media likely in early stationary phase; dependent on DegSU. Not expressed in liquid LB, but only under conditions that promote biofilm formation.
messages: Immunity component of one of 6 LXG toxin-immunity modules in this strain. They promote kin selection, mediate competition in biofilms, and drive spatial segregation of different strains, indicating that LXG toxins may help avoid warfare between strains in biofilms. Mediates intercellular competition during biofilm formation; disruption of the operon disadvantages the bacteria, but overexpression of the cognate immunity protein restores growth in competition with wild-type. In situ neutralizes the toxic effect of cognate toxin YqcG (PubMed:34280190). Probably neutralizes the ability to inhibit growth of cognate toxin YwqJ. Probably does not have immunity protein activity on other LXG toxins (Probable).
messages: Probably interacts with cognate toxin YwqJ but not with other non-cognate LXG toxins. The interaction inhibits the toxic activity of YwqJ.

224308:BSU 07440
gene id: 224308:BSU_07440
uniprot id: O34536
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 03490
gene id: 224308:BSU_03490
uniprot id: A0A6M3Z7E7
uniprot id: Q04747
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Binds 3 phosphopantetheines covalently.
messages: The phosphoserine observed at Ser-999 and Ser-2040 in PubMed:17218307 may result from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.
messages: This protein is a multifunctional enzyme able to activate and polymerize the amino acids Leu, Glu, Asp and Val. Activation sites for these AA consist of individual domains.

224308:BSU 22110
gene id: 224308:BSU_22110
uniprot id: P50845
names: Function
names: Induction
names: Similarity
messages: Belongs to the carbohydrate kinase PfkB family.
messages: Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG).
messages: Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.

224308:BSU 17380
gene id: 224308:BSU_17380
uniprot id: A0A6M4JGM8
uniprot id: P50620
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ribonucleoside diphosphate reductase large chain family.
messages: Essential, at least the last 3 genes of the locus cannot be deleted; could be due to polar effects on downstream ymaB.
messages: Part of the probable nrdI(ymaA)-nrdE-nrdF-ymaB operon. Expression is constitutive but low, dramatically induced by thymidine starvation which requires recA.
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
messages: Tetramer of two alpha and two beta subunits.
messages: Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).

224308:BSU 11940
gene id: 224308:BSU_11940
uniprot id: O31638
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32000
gene id: 224308:BSU_32000
uniprot id: A0A6M4JRJ0
uniprot id: P39071
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Cytoplasm
messages: In response to low temperature.

224308:BSU 22590
gene id: 224308:BSU_22590
uniprot id: P54389
names: Subunit
messages: Interacts with the RNA polymerase core.

224308:BSU 21780
gene id: 224308:BSU_21780
uniprot id: P54156
names: Disruption Phenotype
names: Function
names: Induction
messages: 8-fold induction by growth at 15 degrees Celsius.
messages: Cells grow much more slowly at 15 degrees Celsius.
messages: May play a role in cold adaptation.

224308:BSU 24260
gene id: 224308:BSU_24260
uniprot id: A0A6M3ZDI6
uniprot id: P19672
names: Similarity
messages: Belongs to the TlyA family.

224308:BSU 00470
gene id: 224308:BSU_00470
uniprot id: A0A6M4JC84
uniprot id: P37551
names: Function
names: Similarity
names: Subunit
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family.
messages: Controls the transcription of the pur operon for purine biosynthetic genes, binds to the control region of the operon. DNA binding is inhibited by 5-phosphoribosyl 1-pyrophosphate.
messages: Homodimer.

224308:BSU 19430
gene id: 224308:BSU_19430
uniprot id: A0A6M3ZIK2
uniprot id: O31854
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the adenylate cyclase family. DacB/CdaS subfamily.
messages: Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.
messages: Expressed under control of the sigma-G factor in the forespore.
messages: Forms dimers and probably also hexamers; the dimer may be active while the hexamer may not be active.
messages: No effect on antibiotic sensitivity to the beta-lactam antibiotic cefuroxime (CEF), upon overexpression of the c-di-AMP phosphodiesterase GdpP increased sensitivity to CEF (PubMed:22211522). Double disA-cdaA mutants cannot be made, suggesting they are lethal, while double disA-cdaS and cdaA-cdsS mutants are viable (PubMed:22211522, PubMed:23192352). In single deletions of this gene, germination efficiency is decreased (PubMed:24939848).
messages: One of 3 paralogous diadenylate cyclases (DAC) in this bacteria, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (Probable). Upon expression in E.coli leads to c-di-AMP synthesis (PubMed:23192352, PubMed:24939848). Overexpression of the hyperactive mutant (L44F) in the absence of c-di-AMP phosphodiesterase GdpP leads to growth defects in log phase (long curly cell filaments) that disappear upon sporulation; spore formation is normal, showing sporulation is insensitive to the excess c-di-AMP (PubMed:23192352). In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess.
messages: Probably expressed only during sporulation in the forespore.
messages: Probably oligomerizes.
messages: The N-terminus (residues 1-69) is thought to form 2 alpha-helices that allow dimerization and probable hexamerization; this region inhibits DAC activity of the rest of the protein (PubMed:24939848).

224308:BSU 13210
gene id: 224308:BSU_13210
uniprot id: A0A6M3ZDF8
uniprot id: O34572
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CbiQ family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex YkoCDEF that could transport hydroxymethylpyrimidine (HMP) and/or thiamine. Could also transport other HMP-containing products. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (YkoD), two transmembrane proteins (YkoC and YkoE) and a solute-binding protein (YkoF).

224308:BSU 31680
gene id: 224308:BSU_31680
uniprot id: A0A6M4JL27
uniprot id: P14204
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Phosphorylated by ComP.
messages: Response regulator in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Regulates directly the expression of over 20 genes, including genes of the srfA operon, degQ, rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the regulation of comK transcription, the expression of late competence genes.

224308:BSU 01640
gene id: 224308:BSU_01640
uniprot id: P40408
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Binds with high affinity to both apo-bacillibactin and iron-bacillibactin.
messages: Cytoplasm
messages: In iron-limited conditions, activates expression of the feuABCybbA operon, which encodes the bacillibactin uptake system. Acts by binding directly to a conserved direct repeat element upstream of the feuA promoter. Activity is increased in the presence of bacillibactin.
messages: Repressed by Fur in the presence of iron.

224308:BSU 00910
gene id: 224308:BSU_00910
uniprot id: A0A6M3Z6H3
uniprot id: Q06756
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the IspF family.
messages: Binds 1 divalent metal cation per subunit.
messages: Cells lacking this gene reveal a loss of rod shape, irregular septation, multicompartmentalized cells, and thickened cell walls. It also induces a decrease in isoprene production.
messages: Homotrimer.
messages: Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).

224308:BSU 38880
gene id: 224308:BSU_38880
uniprot id: A0A6M3ZHR2
uniprot id: P55181
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 07010
gene id: 224308:BSU_07010
uniprot id: O31522
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By rhamnogalacturonan type I (RG-I), a component of pectin from the plant cell wall.
messages: Cell membrane
messages: Probable transcription factor regulating the pathway responsible for rhamnogalacturonan depolymerization.

224308:BSU 38229
gene id: 224308:BSU_38229
uniprot id: P0C8M5
names: Disruption Phenotype
names: Function
names: Subunit
messages: Component of the SlrR/SlrA complex.
messages: Defective in biofilm formation.
messages: Required specifically for induction of eps and yqxM operons by antagonizing SinR. Regulates SlrR activity. Controls the initiation of biofilm formation.

224308:BSU 21790
gene id: 224308:BSU_21790
uniprot id: A0A6M4JNI3
uniprot id: P54175
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0073 (Hly-III) family.
messages: Cell membrane
messages: Membrane

224308:BSU 03340
gene id: 224308:BSU_03340
uniprot id: A0A6M4JF09
uniprot id: P94398
names: Caution
names: Function
names: Induction
names: Similarity
messages: Belongs to the GTP cyclohydrolase IV family.
messages: Converts GTP to 7,8-dihydroneopterin triphosphate.
messages: Repressed by zinc, via zur.
messages: Was originally thought to be a zinc uptake system.

224308:BSU 23380
gene id: 224308:BSU_23380
uniprot id: A0A6M3ZIN2
uniprot id: P23630
names: Function
names: Similarity
names: Subunit
messages: Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.
messages: Homodimer.
messages: Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.

224308:BSU 11490
gene id: 224308:BSU_11490
uniprot id: O31601
names: Disruption Phenotype
names: Induction
names: Subcellular Location Annotation
messages: By different stresses, such as ethanol, salt, and phosphate starvation, in a partially sigma-B dependent manner. Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).
messages: Cells lacking this gene display decreased survival rates during salt stress conditions.
messages: Cytoplasm

224308:BSU 26770
gene id: 224308:BSU_26770
uniprot id: O07080
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To E.coli YhgE.

224308:BSU 29790
gene id: 224308:BSU_29790
uniprot id: A0A6M3ZFJ0
uniprot id: P40778
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MurCDEF family.
messages: Cell wall formation.
messages: Cytoplasm

224308:BSU 12610
gene id: 224308:BSU_12610
uniprot id: P54327
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the encapsulin family. Family 3 subfamily.
messages: Possibly a prophage capsid protein.
messages: Secreted

224308:BSU 19730
gene id: 224308:BSU_19730
uniprot id: A0A6M3ZCP9
uniprot id: O34317
names: Similarity
names: Subunit
messages: Belongs to the 3-oxoacid CoA-transferase subunit A family.
messages: Heterodimer of a subunit alpha and a subunit beta.

224308:BSU 09260
gene id: 224308:BSU_09260
uniprot id: A0A6M3Z8V8
uniprot id: P33189
names: Function
names: Similarity
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
messages: Essential for glycerol catabolism.

224308:BSU 27310
gene id: 224308:BSU_27310
uniprot id: A0A6M3ZJK0
uniprot id: O32032
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the transpeptidase family.
messages: Cell membrane
messages: No visible phenotype. Spores have normal morphology, heat-resistance, cortex structure, and germination and outgrowth properties.
messages: Penicillin-binding protein with an unknown catalytic activity. Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis.
messages: Sporulation specific. Expression starts 1 to 2 hours after the initiation of sporulation. Found only in mother cells.

224308:BSU 01990
gene id: 224308:BSU_01990
uniprot id: O31431
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10770
gene id: 224308:BSU_10770
uniprot id: A0A6M3Z993
uniprot id: P54423
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S8 family.
messages: CWBP52 is a serine-type protease that could be involved in proteoglycan peptide bridges.
messages: Cells lacking this gene have no discernible phenotype with respect to sporulation, motility or growth (PubMed:9004506). Significantly increased amounts of BglS, Epr, Vpr, YclQ and YwsB; decreased amounts of AbnA, PorE, Csn, YncM, Yxal and YweA are detected in the extracellular proteome (PubMed:11987133).
messages: In stationary phase.
messages: Inhibited by PMSF.
messages: Proteolytically cleaved to yield CWBP23 and CWBP52.
messages: The CWBP52 polypeptide was originally called CWBP55 based on its apparent molecular weight.
messages: cell wall

224308:BSU 23260
gene id: 224308:BSU_23260
uniprot id: A0A6M4JKZ5
uniprot id: P17620
names: Cofactor
names: Function
names: Similarity
messages: Binds 1 zinc ion per subunit.
messages: Binds 2 divalent metal cations per subunit. Magnesium or manganese.
messages: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
messages: Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
messages: In the C-terminal section; belongs to the GTP cyclohydrolase II family.
messages: In the N-terminal section; belongs to the DHBP synthase family.

224308:BSU 24220
gene id: 224308:BSU_24220
uniprot id: A0A6M3ZDM6
uniprot id: P06534
names: Cofactor
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
messages: Binds 1 Ca(2+) ion per subunit.
messages: Cytoplasm
messages: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box).
messages: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.
messages: Phosphorylated by KinA and KinB.
messages: Stage 0 mutants lack the ability to form the asymmetric septum characteristic of the initiation of the sporulation process.

224308:BSU 39080
gene id: 224308:BSU_39080
uniprot id: P39805
names: Function
names: PTM
names: Similarity
messages: Belongs to the transcriptional antiterminator BglG family.
messages: Mediates positive regulation of the glucanase operon (licST) by functioning as an antiterminator factor of transcription. Prevents termination at terminator lic-t.
messages: Phosphorylated.

224308:BSU 00610
gene id: 224308:BSU_00610
uniprot id: P37559
names: Developmental Stage
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expressed in the mother cell compartment at the second hour of sporulation.
messages: Expression is sigma E-dependent and negatively regulated by spo0A.
messages: Forespore outer membrane
messages: Required for sporulation. Plays an important role in cortex and coat formation.

224308:BSU 35300
gene id: 224308:BSU_35300
uniprot id: A0A6M3ZGR2
uniprot id: P28366
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the SecA family.
messages: Cell membrane
messages: Cytoplasm
messages: May bind 1 zinc ion per subunit.
messages: Membrane raft
messages: Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.
messages: Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.
messages: Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may be involved. Monomer and many different homodimers can be isolated (PubMed:16989859), some of which are not formed in the presence of a synthetic signal peptide. A single SecA monomer interacts with SecY in the channel. Only shows some colocalization with FloA or FloT membrane assemblies (PubMed:27362352).

224308:BSU 12400
gene id: 224308:BSU_12400
uniprot id: A0A6M4JM68
uniprot id: O34578
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.
messages: Cell membrane
messages: Membrane

224308:BSU 38170
gene id: 224308:BSU_38170
uniprot id: A0A6M3ZIW8
uniprot id: P34957
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome c oxidase subunit 2 family.
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water. Major component for energy conversion during vegetative growth. Subunit II transfers the electrons from a quinol to the binuclear center of the catalytic subunit I (By similarity).
messages: Catalyzes quinol oxidation with the concomitant reduction of oxygen to water. Subunit II transfers the electrons from a quinol to the binuclear center of the catalytic subunit I.
messages: Cell membrane
messages: Interacts with FloT.
messages: Membrane
messages: Membrane raft

224308:BSU 06130
gene id: 224308:BSU_06130
uniprot id: O34715
names: Disruption Phenotype
messages: No BsuMI restriction or methylation-related phenotype.

224308:BSU 38060
gene id: 224308:BSU_38060
uniprot id: A0A6M4JQF7
uniprot id: P39608
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FNT transporter (TC 2.A.44) family.
messages: Cell membrane
messages: Membrane

224308:BSU 36990
gene id: 224308:BSU_36990
uniprot id: P45874
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 33239
gene id: 224308:BSU_33239
uniprot id: C0H3R5
names: Induction
messages: Positively regulated by SigO and its coactivator RsoA.

224308:BSU 02290
gene id: 224308:BSU_02290
uniprot id: A0A6M4JEH3
uniprot id: P39822
names: Cofactor
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Prokaryotic type I sub-subfamily.
messages: Binds 1 pyruvoyl group covalently per subunit.
messages: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).
messages: Cell membrane
messages: Disruption mutant contains no phosphatidylethanolamine and accumulates phosphatidylserine, but it grows well without supplementation of divalent cations.
messages: Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.
messages: Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.

224308:BSU 18350
gene id: 224308:BSU_18350
uniprot id: A0A6M3ZBB2
uniprot id: P39844
names: Activity Regulation
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S13 family.
messages: Catalyzes DD-carboxypeptidase and transpeptidation reactions.
messages: Expression is sigma H-dependent.
messages: Expression starts at the end of the exponential phase and peaks two hours into sporulation. There is no enzyme activity in spores.
messages: Inhibited by cephaloridine.
messages: Membrane raft
messages: Secreted

224308:BSU 06760
gene id: 224308:BSU_06760
uniprot id: O31504
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the methyltransferase superfamily.
messages: Between 94.7% and 99.7% of the 5'-GACGAG-3' motifs are methylated on A-5 in strain NCIMB 3610 and PY79 respectively. The sites are enriched on the left chromosomal arm.
messages: No visible growth defects, no A-5 methylation of 5'-GACGAG-3' in strains NCIMB 3610 or PY79. Decreased promoter activity for promoters with the target sequence very close to the -35 SigA-binding box (scpA, hbs, rnhC, yumC and zapA).
messages: Recognizes the double-stranded sequence 5'-GACGAG-3' and methylates A-5, yielding m6A. m6A methylation functions as a transcriptional modifier, promoting transcription of a number of genes (at least scpA, hbs, rnhC, yumC and zapA). One studied mechanism is via transcriptional repressor ScoC (also called hpr) which binds to non-methylated scpA promoter; when the m6A target is methylated ScoC no longer binds and scpA transcription is up-regulated. Other mechanisms for gene expression regulation probably exist. Binds DNA with and without the target sequence. Although it resembles a restriction-modification system, it does not have detectable endonuclease activity under tested conditions (PubMed:32324221). A gamma subtype methylase (PubMed:12654995).

224308:BSU 24030
gene id: 224308:BSU_24030
uniprot id: A0A6M3ZDK6
uniprot id: P37942
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the 2-oxoacid dehydrogenase family.
messages: Binds 1 lipoyl cofactor covalently.
messages: Forms a 24-polypeptide structural core with octahedral symmetry.
messages: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 28340
gene id: 224308:BSU_28340
uniprot id: P94560
names: Induction
messages: By phosphate starvation, via the alternative sigma factor sigma-B.

224308:BSU 06120
gene id: 224308:BSU_06120
uniprot id: O34778
names: Disruption Phenotype
messages: No BsuMI restriction or methylation-related phenotype.

224308:BSU 32290
gene id: 224308:BSU_32290
uniprot id: A0A6M4JLR2
uniprot id: O32125
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the HAD-like hydrolase superfamily. NagD family.
messages: Catalyzes the dephosphorylation of 2-6 carbon acid sugars in vitro.
messages: Catalyzes the hydrolysis of various purine and pyrimidine 5'-nucleotides, showing preference for 5'-nucleoside monophosphates and exhibiting the highest catalytic activity toward 5'-XMP (PubMed:27907199). Shows also a relatively high phosphohydrolase activity toward the nucleotide precursors ribose-5-phosphate (R5P) and 5-phosphoribosyl-1-pyrophosphate (PRPP), and toward the non-natural substrate p-nitrophenyl phosphate (pNPP) (PubMed:27907199).
messages: Cotranscribed with the two upstream genes, yutD and yutE. YutF overproduction increases the level of yutDEF operon expression, and this up-regulation is enhanced in the presence of inorganic phosphate.
messages: Cytoplasm
messages: Divalent metal ions. Mg(2+) is the most effective.
messages: Homodimer.
messages: Inactivation of the gene has essentially no effect on cell growth in rich or minimal medium but results in a drop in pNPP hydrolysis in the crude extracts of B.subtilis cells to undetectable levels.

224308:BSU 38040
gene id: 224308:BSU_38040
uniprot id: P07819
names: Similarity
messages: Belongs to the glycosyl hydrolase 32 family.

224308:BSU 29180
gene id: 224308:BSU_29180
uniprot id: A0A6M4JKB7
uniprot id: P80885
names: Similarity
names: Subunit
messages: Belongs to the pyruvate kinase family.
messages: Homotetramer.
messages: In the C-terminal section; belongs to the PEP-utilizing enzyme family.

224308:BSU 33300
gene id: 224308:BSU_33300
uniprot id: A0A6M3ZGV6
uniprot id: P49937
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex FhuBGCD involved in iron(3+)-hydroxamate import. Responsible for the translocation of the substrate across the membrane (By similarity).
messages: The complex is composed of an ATP-binding protein (FhuC), two transmembrane proteins (FhuB and FhuG) and a solute-binding protein (FhuD or YxeB).

224308:BSU 09240
gene id: 224308:BSU_09240
uniprot id: P54607
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.

224308:BSU 01140
gene id: 224308:BSU_01140
uniprot id: A0A6M4JER6
uniprot id: P70981
names: Function
names: Similarity
messages: Belongs to the peptidase S33 family.
messages: Probable aminopeptidase.

224308:BSU 23280
gene id: 224308:BSU_23280
uniprot id: A0A6M3ZIR2
uniprot id: P17618
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Binds 1 zinc ion.
messages: Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.
messages: Homotetramer.
messages: In the C-terminal section; belongs to the HTP reductase family.
messages: In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.

224308:BSU 19780
gene id: 224308:BSU_19780
uniprot id: P42089
names: Function
messages: May be involved in maturation of the outermost layer of the spore.

224308:BSU 18490
gene id: 224308:BSU_18490
uniprot id: A0A6M4JKK8
uniprot id: P0CI76
names: Function
names: Subunit
messages: Homodimer.
messages: Plays a role in DNA replication and termination (fork arrest mechanism). Two dimers of rtp bind to the two inverted repeat regions (IRI and IRII) present in the termination site. The binding of each dimer is centered on an 8 bp direct repeat.

224308:BSU 24290
gene id: 224308:BSU_24290
uniprot id: A0A6M3ZDX0
uniprot id: P54522
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the XseB family.
messages: Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
messages: Cytoplasm
messages: Heterooligomer composed of large and small subunits.

224308:BSU 40560
gene id: 224308:BSU_40560
uniprot id: P37488
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32830
gene id: 224308:BSU_32830
uniprot id: A0A6M3ZGH3
uniprot id: O32177
names: Function
names: Induction
names: Similarity
messages: Belongs to the thiolase-like superfamily. Thiolase family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 36480
gene id: 224308:BSU_36480
uniprot id: A0A6M3ZIV2
uniprot id: P94574
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 09030
gene id: 224308:BSU_09030
uniprot id: P54587
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 10530
gene id: 224308:BSU_10530
uniprot id: O07564
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.
messages: Induced by neotrehalosadiamine.
messages: Involved in the biosynthesis of kanosamine (3-amino-3-deoxy-D-glucose), which is known to have antibiotic and antifungal properties, and to be a precursor of the antibiotic neotrehalosadiamine (3,3'-diamino-3,3'-dideoxy-alpha,beta-trehalose (NTD)). Catalyzes the oxidation of glucose 6-phosphate to 3-oxo-D-glucose 6-phosphate. It can only use NAD.
messages: The production of neotrehalosadiamine is dormant in the wild-type strain. A mutation in the beta subunit of RNA polymerase activates the production of the neotrehalosadiamine (PubMed:14612444).

224308:BSU 31340
gene id: 224308:BSU_31340
uniprot id: O05245
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 32900
gene id: 224308:BSU_32900
uniprot id: O32184
names: Caution
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Could be the product of a pseudogene. This sequence is shorter than orthologs and lacks the NAD(P)-binding motif.

224308:BSU 23610
gene id: 224308:BSU_23610
uniprot id: A0A6M4JND5
uniprot id: P54570
names: Cofactor
names: Function
names: Similarity
messages: Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process (By similarity).
messages: Belongs to the Nudix hydrolase family.
messages: Belongs to the Nudix hydrolase family. NudF subfamily.
messages: Binds 3 Mg(2+) ions per subunit.

224308:BSU 29480
gene id: 224308:BSU_29480
uniprot id: P37876
names: Similarity
messages: To type I restriction system adenine methylases.

224308:BSU 28450
gene id: 224308:BSU_28450
uniprot id: P08064
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome b558 family.
messages: Cell membrane
messages: Di-heme cytochrome of the succinate dehydrogenase complex.
messages: Part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein and cytochrome b-558.

224308:BSU 21980
gene id: 224308:BSU_21980
uniprot id: A0A6M4JNI8
uniprot id: P54163
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family. Q precursor transporter subfamily.
messages: Cell membrane
messages: Involved in the import of queuosine (Q) precursors, required for Q precursor salvage.

224308:BSU 02240
gene id: 224308:BSU_02240
uniprot id: A0A6M3Z6Z9
uniprot id: P39790
names: Caution
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase S1B family.
messages: Called 'metalloprotease', but clearly belongs to the S1B family of serine proteases.
messages: Monomer.
messages: Secreted

224308:BSU 34930
gene id: 224308:BSU_34930
uniprot id: A0A6M4JPI3
uniprot id: O34459
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.
messages: Cytoplasm
messages: Heteromultimer composed of HisG and HisZ subunits.
messages: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine (By similarity).
messages: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.
messages: This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.

224308:BSU 28250
gene id: 224308:BSU_28250
uniprot id: A0A6M3ZF47
uniprot id: P94568
names: Function
names: Similarity
names: Subunit
messages: Belongs to the LeuD family. LeuD type 1 subfamily.
messages: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.
messages: Heterodimer of LeuC and LeuD.

224308:BSU 13390
gene id: 224308:BSU_13390
uniprot id: O34755
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 2 family.
messages: Cell membrane

224308:BSU 03840
gene id: 224308:BSU_03840
uniprot id: A0A6M3Z7Z0
uniprot id: P94422
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Membrane

224308:BSU 16140
gene id: 224308:BSU_16140
uniprot id: A0A6M3ZAZ8
uniprot id: P39776
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the 'phage' integrase family. XerC subfamily.
messages: Cytoplasm
messages: Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.
messages: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.

224308:BSU 05080
gene id: 224308:BSU_05080
uniprot id: P96655
names: Similarity
messages: Belongs to the UPF0173 family.

224308:BSU 40030
gene id: 224308:BSU_40030
uniprot id: P42101
names: Induction
names: Similarity
messages: Belongs to the polysaccharide pyruvyl transferase family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 22420
gene id: 224308:BSU_22420
uniprot id: A0A6M3ZIK8
uniprot id: P52998
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the pantothenate synthetase family.
messages: Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.
messages: Cytoplasm
messages: Homodimer.
messages: The reaction proceeds by a bi uni uni bi ping pong mechanism.

224308:BSU 03250
gene id: 224308:BSU_03250
uniprot id: A0A6M3ZCH2
uniprot id: P94395
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0718 family.
messages: Cell membrane
messages: Membrane

224308:BSU 37310
gene id: 224308:BSU_37310
uniprot id: P46908
names: Cofactor
names: Domain
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: By anaerobiosis.
messages: Cytoplasm
messages: Homodimer.
messages: It is involved in the activation of genes necessary for anaerobic respiration.
messages: The cysteine cluster which is probably involved in the coordination of the [4Fe-4S] cluster is located at the C-terminal part of the protein.

224308:BSU 28000
gene id: 224308:BSU_28000
uniprot id: A0A6M4JLL8
uniprot id: Q01463
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MinC family.
messages: Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.
messages: Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization. The MinCD complex plays an important role in asymmetric septum formation during sporulation of B.subtilis cells.
messages: Interacts with MinD and FtsZ.

224308:BSU 24610
gene id: 224308:BSU_24610
uniprot id: P06533
names: Function
names: Subunit
messages: Homotetramer in the absence of SinI. Heterodimer with SinI. Interaction with SinI disrupts the SinR tetramer and its repressor activity. Interacts with hpr.
messages: Negative as well as positive regulator of alternate developmental processes that are induced at the end of vegetative growth in response to nutrient depletion. Binds to the alkaline protease (aprE) gene at two sites. Also acts as a repressor of the key sporulation gene spo0A. Negatively regulates transcription of the eps operon, which is responsible for the biosynthesis of an exopolysaccharide involved in biofilm formation; therefore it could govern the transition between a state in which bacteria swim or swarm and a state in which bacteria assemble into multicellular communities. Acts with Hpr as a corepressor of epr expression. Also negatively regulates transcription of the lutABC operon, which is required for lactate utilization. Repressor activity is regulated by SinI.

224308:BSU 06269
gene id: 224308:BSU_06269
uniprot id: O24817
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37060
gene id: 224308:BSU_37060
uniprot id: A0A6M3ZHC6
uniprot id: Q03221
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the thymidine kinase family.
messages: Cytoplasm
messages: Homotetramer.

224308:BSU 01630
gene id: 224308:BSU_01630
uniprot id: P40409
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Cytoplasm
messages: Involved in the uptake of iron.
messages: Membrane raft
messages: Part of the ABC transporter complex FeuABC/YusV involved in import of the catecholate siderophores bacillibactin and enterobactin.
messages: Repressed by fur. Induced by Btr in iron-limited conditions.
messages: Strains lacking this gene show a reduction in growth stimulation by the catecholate siderophores enterobactin and bacillibactin.
messages: The complex is composed of one ATP-binding protein (YusV), two transmembrane proteins (FeuB and FeuC) and a solute-binding protein (FeuA).

224308:BSU 33820
gene id: 224308:BSU_33820
uniprot id: A0A6M3ZKW1
uniprot id: O34878
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.
messages: Cell membrane
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane.
messages: Membrane
messages: Repressed by OpcR.
messages: The complex is composed of two ATP-binding proteins (OpuCA), two transmembrane proteins (OpuCB and OpuCD) and a solute-binding protein (OpuCC).

224308:BSU 07220
gene id: 224308:BSU_07220
uniprot id: O31541
names: Activity Regulation
names: Function
names: Induction
names: Subunit
messages: Binding to the yetM cis sequence is clearly inhibited by kaempferol, morin, apigenin and luteolin, slightly inhibited by quercetin and galangin, but no inhibition is observed with the other flavonoids (PubMed:19329649). Flavonoid binding may induce conformational changes and modulate interaction with DNA (PubMed:35367827).
messages: Homodimer (PubMed:19329649, PubMed:35367827). The N- and C-terminal helices from both subunits stabilize YetL dimer via extensive intersubunit interactions (PubMed:35367827).
messages: Negatively regulates yetM expression and its own expression (PubMed:19329649). Binds specifically to corresponding single sites in the divergent yetL and yetM promoter regions, with higher affinity to the yetM region (PubMed:19329649). Recognizes a 28-mer operator of double-stranded DNA that contains a palindromic sequence (PubMed:35367827).
messages: Partially autorepressed.

224308:BSU 40420
gene id: 224308:BSU_40420
uniprot id: A0A6M4JR71
uniprot id: P29726
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the adenylosuccinate synthetase family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Cytoplasm
messages: Homodimer.
messages: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

224308:BSU 11330
gene id: 224308:BSU_11330
uniprot id: A0A6M4JKF8
uniprot id: O34746
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the thiolase-like superfamily. FabH family.
messages: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Has some substrate specificity for branched chain acyl-CoA, determining the biosynthesis of branched-chain of fatty acids instead of straight-chain.
messages: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.
messages: Cytoplasm
messages: Down-regulated by FapR.
messages: Homodimer.
messages: The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.

224308:BSU 05760
gene id: 224308:BSU_05760
uniprot id: O05500
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 24400
gene id: 224308:BSU_24400
uniprot id: P49781
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 28790
gene id: 224308:BSU_28790
uniprot id: A0A6M3ZI76
uniprot id: P94524
names: Induction
names: Similarity
messages: Belongs to the ribulokinase family.
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 28490
gene id: 224308:BSU_28490
uniprot id: A0A6M4JMH0
uniprot id: P14951
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the UvrC family.
messages: Cytoplasm
messages: Interacts with UvrB in an incision complex.
messages: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.

224308:BSU 09560
gene id: 224308:BSU_09560
uniprot id: O07586
names: Function
names: Miscellaneous
messages: Binds a DNA inverted repeat of copZA operon.
messages: Transcriptional activator of the copZA operon.

224308:BSU 13990
gene id: 224308:BSU_13990
uniprot id: P16497
names: Function
messages: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP.

224308:BSU 21060
gene id: 224308:BSU_21060
uniprot id: O31947
names: Subunit
messages: Octamer.

224308:BSU 27500
gene id: 224308:BSU_27500
uniprot id: A0A6M3ZF60
uniprot id: O35020
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MnmA/TRMU family.
messages: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 40770
gene id: 224308:BSU_40770
uniprot id: A0A6M4JRB4
uniprot id: P23054
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. TCR/Tet family.
messages: Cell membrane
messages: Membrane
messages: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter.

224308:BSU 05280
gene id: 224308:BSU_05280
uniprot id: A0A6M3ZD28
uniprot id: P96672
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 27700
gene id: 224308:BSU_27700
uniprot id: A0A6M3ZIQ4
uniprot id: O32052
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the YajC family.
messages: Cell membrane
messages: Membrane
messages: Part of the SecDF-YidC-YajC translocase complex. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL).
messages: The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions.

224308:BSU 35810
gene id: 224308:BSU_35810
uniprot id: A0A6M4JPS4
uniprot id: P39570
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
messages: Cell membrane
messages: Expressed in the forespore compartment of the developing sporangium.
messages: Involved in the response to the germinative mixture of L-asparagine, glucose, fructose and potassium ions (AGFK). Could be an amino acid transporter. Cannot stimulate germination in the absence of gerD and gerK gene products (fructose and glucose receptors, respectively).
messages: Membrane

224308:BSU 31600
gene id: 224308:BSU_31600
uniprot id: A0A6M3ZG28
uniprot id: Q9K2S2
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family.
messages: Cell membrane
messages: Forms a heterooligomeric complex that consists of seven subunits: MrpA, MrpB, MrpC, MrpD, MrpE, MrpF and MrpG.
messages: Membrane
messages: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual Na(+)/H(+) antiporter, although the six other Mrp proteins are all required for Na(+)/H(+) antiport activity and Na(+) resistance. MrpA is required for initiation of sporulation when external Na(+) concentration increases. Also transports Li(+) but not K(+), Ca(2+) or Mg(2+).
messages: Mrp-dependent antiport apparently occurs by a secondary, proton motive force-dependent mechanism, but the similarity of several Mrp proteins to membrane-embedded subunits of energy-coupled NADH dehydrogenase complexes raises the possibility that there is a capacity for electron transport that could provide a primary energy coupling option for Mrp functions.

224308:BSU 06770
gene id: 224308:BSU_06770
uniprot id: O34469
names: Similarity
messages: Belongs to the helicase family.

224308:BSU 11170
gene id: 224308:BSU_11170
uniprot id: Q796P5
names: Similarity
messages: Belongs to the oxygen-dependent FAD-linked oxidoreductase family.

224308:BSU 16370
gene id: 224308:BSU_16370
uniprot id: A0A6M3ZAT4
uniprot id: P35537
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliR/MopE/SpaR family.
messages: Cell membrane
messages: Membrane
messages: Role in flagellar biosynthesis.

224308:BSU 30950
gene id: 224308:BSU_30950
uniprot id: A0A6M3ZFX3
uniprot id: P39125
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.
messages: Expressed exclusively on media containing carbon sources that allow efficient sporulation.
messages: Synthesizes alpha-1,4-glucan chains using ADP-glucose.

224308:BSU 40370
gene id: 224308:BSU_40370
uniprot id: C0SP91
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: 5'->3' double-stranded DNA exonuclease (By similarity). May play a role in mutation mismatch repair (MMR) (By similarity). Required for accurate coordination of cell division with DNA replication (PubMed:21169496). May play a role in cell wall metabolism (PubMed:21169496).
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 metal ions per subunit. The endogenous metal is unknown.
messages: Expressed during exponential growth.
messages: More frequent cell division over unsegregated chromosomes (PubMed:21169496). Increases frequency of nucleoid bisection (PubMed:21169496). Induces SOS induction in a subpopulation of cells (PubMed:21169496). Slightly elongated cells (PubMed:21169496). More sensitive to some cephalosporin antibiotics, such as cefotaxime, cefuroxime, and cefmetazole but not to cefoxitin or cefoperazone (PubMed:21169496). Increased sensitivity to moxalactam, a beta-lactam closely related to the cephalosporins, but not to the penicillin-like beta-lactam carbenicillin (PubMed:21169496).

224308:BSU 36400
gene id: 224308:BSU_36400
uniprot id: A0A6M4JPW0
uniprot id: P39752
names: Function
names: Similarity
messages: Belongs to the flagella basal body rod proteins family.
messages: Not required for motility.

224308:BSU 32160
gene id: 224308:BSU_32160
uniprot id: O32113
names: Disruption Phenotype
names: Similarity
messages: Belongs to the HesB/IscA family.
messages: Not essential for growth on minimal medium.

224308:BSU 09570
gene id: 224308:BSU_09570
uniprot id: A0A6M4JK02
uniprot id: O07587
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
messages: Cytoplasm

224308:BSU 28780
gene id: 224308:BSU_28780
uniprot id: A0A6M3ZF01
uniprot id: P94525
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the aldolase class II family. AraD/FucA subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Involved in the degradation of L-arabinose. Catalyzes the interconversion of L-ribulose 5-phosphate (LRu5P) and D-xylulose 5-phosphate (D-Xu5P) via a retroaldol/aldol mechanism (carbon-carbon bond cleavage analogous to a class II aldolase reaction).
messages: Transcription is repressed by glucose and by the binding of AraR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of AraR to the DNA, thus allowing expression of the gene.

224308:BSU 05480
gene id: 224308:BSU_05480
uniprot id: P96692
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the nitroreductase family.
messages: Cytoplasm
messages: Homodimer.
messages: Putative nitroreductase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 27390
gene id: 224308:BSU_27390
uniprot id: A0A6M3ZHU3
uniprot id: O34634
names: Caution
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the YqgF HJR family.
messages: Belongs to the YqgF nuclease family.
messages: Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.
messages: Cytoplasm
messages: Not essential, it can be disrupted.
messages: Was originally suggested to be a nuclease that resolves Holliday junction intermediates during genetic recombination.

224308:BSU 06870
gene id: 224308:BSU_06870
uniprot id: O31511
names: Similarity
messages: Belongs to the limonene-1,2-epoxide hydrolase family.

224308:BSU 09590
gene id: 224308:BSU_09590
uniprot id: A0A6M3Z8Y4
uniprot id: O07589
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0053 family.
messages: Cell membrane
messages: Membrane

224308:BSU 39330
gene id: 224308:BSU_39330
uniprot id: A0A6M4JQW9
uniprot id: P42293
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycosyl hydrolase 43 family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Homodimer.
messages: Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the internal cleavage of alpha-(1->5)-L-arabinofuranosyl residues of the alpha-1,5-L-arabinan to produce arabino-oligosaccharides and L-arabinose. It is also active toward linear branched sugar beet arabinan, and pectin from apple.
messages: No visible phenotype. Double mutant abn2/abnA induce an almost complete loss of this activity.
messages: Repressed by glucose, and induced by pectin and arabinan.
messages: Secreted

224308:BSU 00130
gene id: 224308:BSU_00130
uniprot id: A0A6M3Z6Z1
uniprot id: P37464
names: Caution
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
messages: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
messages: Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
messages: Cytoplasm
messages: Homodimer. The tRNA molecule binds across the dimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 18750
gene id: 224308:BSU_18750
uniprot id: O34535
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 31690
gene id: 224308:BSU_31690
uniprot id: Q99027
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
messages: Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in ComA, thus activating it.
messages: Cell membrane
messages: Sensor in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of SpoIIJ. Probably activates ComA by phosphorylation.
messages: The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.

224308:BSU 30720
gene id: 224308:BSU_30720
uniprot id: A0A6M4JLE4
uniprot id: O34505
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome ubiquinol oxidase subunit 2 family.
messages: Cell membrane
messages: May have a role in sporulation. Can compensate for the loss of cytochrome aa3.
messages: Membrane

224308:BSU 33940
gene id: 224308:BSU_33940
uniprot id: A0A6M3ZG39
uniprot id: P09124
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
messages: Cells lacking this gene are unable to grow in medium containing glucose as a sole carbon source but presents the same growth rate as the wild-type in asparagine-containing medium.
messages: Cytoplasm
messages: Homotetramer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: In response to oxidative stress, the active site Cys likely reacts with bacillithiol (BSH) to form mixed disulfides to protect the Cys residue against overoxidation. S-bacillithiolation presumably leads to loss of catalytic activity. Debacillithiolation by monothiol bacilliredoxin BrxC restores the activity.
messages: Involved in the glycolysis. Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
messages: Repressed by CggR and indirectly stimulated by CcpA.

224308:BSU 00350
gene id: 224308:BSU_00350
uniprot id: O31414
names: Similarity
messages: Belongs to the UPF0213 family.

224308:BSU 32880
gene id: 224308:BSU_32880
uniprot id: A0A6M4JLG2
uniprot id: O32182
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Membrane

224308:BSU 29730
gene id: 224308:BSU_29730
uniprot id: A0A6M3ZM97
uniprot id: P39063
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MotA family.
messages: Cell membrane
messages: May be involved in some transport function.
messages: Membrane

224308:BSU 30410
gene id: 224308:BSU_30410
uniprot id: A0A6M4JNS3
uniprot id: O35005
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Expressed early in the stationary phase.
messages: Membrane
messages: Negatively regulated by YtrA.
messages: Part of the ABC transporter complex YtrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.
messages: The complex is composed of 2 ATP-binding proteins (YtrB and YtrE), 2 transmembrane proteins (YtrC and YtrD) and a solute-binding protein (YtrF).

224308:BSU 28580
gene id: 224308:BSU_28580
uniprot id: A0A6M3ZEY8
uniprot id: P94545
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.
messages: Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity.
messages: Homodimer.

224308:BSU 18370
gene id: 224308:BSU_18370
uniprot id: O34474
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
messages: Cell membrane

224308:BSU 35830
gene id: 224308:BSU_35830
uniprot id: A0A6M3ZGW1
uniprot id: C0SPB2
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane

224308:BSU 30100
gene id: 224308:BSU_30100
uniprot id: O34371
names: Function
names: Induction
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.
messages: May play a role in the degradation of type I rhamnogalacturonan derived from plant cell walls.
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 25320
gene id: 224308:BSU_25320
uniprot id: A0A6M3ZE10
uniprot id: P46347
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the endoribonuclease YbeY family.
messages: Binds 1 zinc ion.
messages: Cytoplasm
messages: Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.

224308:BSU 05690
gene id: 224308:BSU_05690
uniprot id: A0A6M3Z7U8
uniprot id: O05493
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.
messages: Cell membrane
messages: Membrane

224308:BSU 08080
gene id: 224308:BSU_08080
uniprot id: A0A6M4JJL7
uniprot id: O31550
names: Cofactor
names: Similarity
messages: Belongs to the 2-oxoacid dehydrogenase family.
messages: Binds 1 lipoyl cofactor covalently.

224308:BSU 00520
gene id: 224308:BSU_00520
uniprot id: A0A6M3ZBP3
uniprot id: P14194
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Cells sporulate poorly at 48 degrees Celsius.
messages: Expressed at the end of exponential growth under conditions in which the enzymes of the TCA cycle are repressed.
messages: Not required for exponential growth; probably functions in vegetatively growing cells, maybe required for accurate translation under stress conditions.
messages: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.
messages: Part of the ribosome (presumably the 50S subunit) under heat-stress but not control growth conditions. Binds 5S rRNA.
messages: This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.

224308:BSU 03900
gene id: 224308:BSU_03900
uniprot id: A0A6M3ZAN4
uniprot id: P94427
names: Similarity
messages: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 06400
gene id: 224308:BSU_06400
uniprot id: A0A6M4JH09
uniprot id: O34624
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0316 family.
messages: Cell membrane
messages: Membrane

224308:BSU 11660
gene id: 224308:BSU_11660
uniprot id: P25053
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the thiazole tautomerase family.
messages: Catalyzes the irreversible aromatization of 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate (cThz*-P) to 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (cThz-P), a step in the biosynthesis of the thiazole phosphate moiety of thiamine. Cannot use cThz* as substrate, indicating that the phosphate group is essential. Has no thiamine phosphate synthase activity, despite a high sequence similarity with ThiE.
messages: Homodimer.
messages: Inactivation of this gene causes a delay in sporulation, but does not affect cell growth and the production of extracellular enzymes.
messages: Strongly repressed by thiamine.
messages: Was originally described as a regulatory protein involved in the regulation of the production of extracellular enzymes.

224308:BSU 22290
gene id: 224308:BSU_22290
uniprot id: Q7WY65
names: Developmental Stage
names: Induction
names: Subcellular Location Annotation
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination.
messages: Expression is sigma G-dependent.
messages: Spore core

224308:BSU 41030
gene id: 224308:BSU_41030
uniprot id: A0A6M4JRA7
uniprot id: Q01620
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A probable RNA chaperone. Forms a complex with KhpA which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.
messages: Belongs to the KhpB RNA-binding protein family.
messages: Cytoplasm
messages: Forms a complex with KhpA.
messages: Has an N-terminal Jag-N domain and 2 RNA-binding domains (KH and R3H).

224308:BSU 11620
gene id: 224308:BSU_11620
uniprot id: O31613
names: Similarity
messages: Belongs to the pseudouridine synthase RluA family.

224308:BSU 14910
gene id: 224308:BSU_14910
uniprot id: A0A6M4JFZ3
uniprot id: P24012
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the cytochrome c oxidase subunit 3 family.
messages: Cell membrane
messages: Membrane

224308:BSU 27180
gene id: 224308:BSU_27180
uniprot id: O05400
names: Induction
names: Similarity
messages: Belongs to the methyltransferase superfamily.
messages: By the natural cationic antimicrobial peptide LL-37 and the synthetic cationic antimicrobial peptide poly-L-lysine (PLL).

224308:BSU 16420
gene id: 224308:BSU_16420
uniprot id: A0A6M4JGE4
uniprot id: Q05522
names: Domain
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CheB family.
messages: Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.
messages: Cytoplasm
messages: Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.
messages: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR. B.subtilis has an effective methylation-independent adaptation system but must utilize the methylation system for adaptation to high concentrations of attractant.
messages: Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.

224308:BSU 09680
gene id: 224308:BSU_09680
uniprot id: A0A6M3Z8Y5
uniprot id: O07553
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter family.
messages: Cell membrane
messages: Is a secondary, electrogenic Na(+)/H(+) antiporter that catalyzes Na(+) uptake and proton efflux. Makes modest contributions to pH homeostasis in the alkaline range of pH but is not contributor to Na(+) resistance. Appears to have a repressive effect on growth and on alkaline phosphatases production in the presence of sodium, by affecting the transcription of the phoP/phoR two-component regulatory system.
messages: Membrane
messages: The antiport activity is markedly inhibited by both valinomycin and CCCP, and modestly by nigericin.

224308:BSU 36420
gene id: 224308:BSU_36420
uniprot id: P15281
names: Function
messages: This protein regulates the transcription of sigK, which encodes mother cell chamber RNA polymerase sigma-factor (sigma K).

224308:BSU 02530
gene id: 224308:BSU_02530
uniprot id: O31466
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: By forming a complex with tryptophan-activated TRAP, and masking its RNA binding site, it inhibits TRAP's RNA binding ability, thereby abolishing TRAP regulation of gene expression, leading to antitermination and increased trp operon expression. AT acts by competing with messenger RNA for the RNA binding domain of TRAP.
messages: By uncharged Trp tRNA, via the T-box transcription antitermination mechanism.
messages: Cytoplasm
messages: Homopentamer or homohexamer.

224308:BSU 21490
gene id: 224308:BSU_21490
uniprot id: O31989
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expressed during both exponential and post-exponential growth (at protein level).
messages: Is essential and sufficient to confer resistance to the bacteriocin sublancin 168.
messages: Loss of resistance to the bacteriocin sublancin 168.

224308:BSU 22400
gene id: 224308:BSU_22400
uniprot id: A0A6M3ZGN4
uniprot id: P54394
names: Function
names: Similarity
messages: 3'-5' exonuclease.
messages: Belongs to the helicase family. DinG subfamily. Type 2 sub-subfamily.

224308:BSU 03730
gene id: 224308:BSU_03730
uniprot id: A0A6M4JF45
uniprot id: P94411
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 17800
gene id: 224308:BSU_17800
uniprot id: O31813
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 30510
gene id: 224308:BSU_30510
uniprot id: O34705
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the AB hydrolase superfamily.
messages: Cells lacking this gene entirely lose the ability to produce bacilysocin and display a 10-fold reduction in the ability to form spores.
messages: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis reaction of one acyl bond in phosphatidylcholine in vitro (actual cleavage point is unknown), resulting in lysophosphatidylcholine.

224308:BSU 25660
gene id: 224308:BSU_25660
uniprot id: A0A6M4JL68
uniprot id: P54374
names: Function
names: Similarity
names: Subunit
messages: Belongs to the shikimate dehydrogenase family.
messages: Homodimer.
messages: Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).

224308:BSU 27810
gene id: 224308:BSU_27810
uniprot id: A0A6M3ZF92
uniprot id: O32060
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family.
messages: Cell membrane
messages: Membrane

224308:BSU 22930
gene id: 224308:BSU_22930
uniprot id: A0A6M4JLD2
uniprot id: P50739
names: Developmental Stage
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis SleB could not be detected with anti-B.cereus SleB antiserum and vice versa.
messages: Belongs to the SleB family.
messages: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components.
messages: Expressed during sporulation and active during germination. Exists as mature but inactive form in the dormant spore.
messages: Expression is sigma G-dependent.
messages: Forespore

224308:BSU 31530
gene id: 224308:BSU_31530
uniprot id: A0A6M4JMG3
uniprot id: O05251
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of a two-component regulatory system MalK/MalR. Activates transcription of maeA, maeN and yflS in presence of malate by binding to their promoter region.
messages: Phosphorylated and activated by MalK.

224308:BSU 17640
gene id: 224308:BSU_17640
uniprot id: A0A6M3ZKU1
uniprot id: P94494
names: Function
names: Similarity
messages: Belongs to the alanine racemase family.
messages: Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.

224308:BSU 39310
gene id: 224308:BSU_39310
uniprot id: P42295
names: Disruption Phenotype
messages: Deletion of the yxiB-yxiC-yxiD-yxxD-yxxE operon has no visible growth phenotype, however it is out-competed by wild-type cells.

224308:BSU 18060
gene id: 224308:BSU_18060
uniprot id: A0A6M4JGU5
uniprot id: Q45063
names: Similarity
messages: Belongs to the HesB/IscA family.

224308:BSU 29690
gene id: 224308:BSU_29690
uniprot id: A0A6M3ZFB5
uniprot id: P39065
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Activity is sensitive to salt concentration, a high concentration of KCL (500 mM) is needed for complete inactivation.
messages: Belongs to the acetyltransferase family.
messages: Does not impair growth on acetate. Disruption together with other acetoin utilization genes acuB and acuC results in poor growth and sporulation on acetoin or butanediol. Triple deletion of acuA, acuC and srtN improves the growth on acetate but it does not reach that of wild-type under low-acetate conditions.
messages: Expression is maximal in stationary phase and is repressed by the addition of glucose to the growth medium. CcpA protein is required for glucose repression. Acetoin does not act as an inducer. Up-regulated during the 80 to 100 minutes of spore germination and into the outgrowth phase.
messages: Monomer.
messages: Part of the acuABC operon, which is possibly involved in the breakdown of acetoin and butanediol. Acts as an acetyltransferase inactivating acetyl-CoA synthetase AcsA via acetylation at a Lys residue.

224308:BSU 03630
gene id: 224308:BSU_03630
uniprot id: A0A6M3ZAI4
uniprot id: P94404
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: A triple bsdB-bsdC-bsdD deletion mutant no longer converts vanillin to guaiacol, the conversion stops at vanillic acid (PubMed:26658822).
messages: Belongs to the UbiX/PAD1 family. YclB subfamily.
messages: Homododecamer.
messages: Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives under both aerobic and anaerobic conditions (PubMed:18388975). Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for phenolic acid decarboxylase (By similarity).
messages: Involved in the non-oxidative decarboxylation and detoxification of phenolic derivatives. Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for phenolic acid decarboxylase.
messages: It is not known, if phenolic acid decarboxylase forms a complex composed of BsdB, BsdC and BsdD. The term subunit is often used in reference to the operon, however there is no experimental evidence to prove the existence of the complex.
messages: Up-regulated by salicylate via the transcriptional regulator BsdA.

224308:BSU 33250
gene id: 224308:BSU_33250
uniprot id: O34686
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Cells lacking this gene exhibit a slight increase in OxdC accumulation under acidic growth conditions and a huge increase under nonstress growth conditions.
messages: Expression activated by ComK (PubMed:11918817). Positively regulated by SigO and its coactivator RsoA.
messages: Negatively regulates RNA polymerase sigma factor SigO-dependent transcription. Prevents the expression or secretion of OxdC under nonstress conditions. May act as an anti-sigma factor.
messages: Preferentially expressed in cells competent for DNA transformation; that is 5-15% of the population (PubMed:11918817).

224308:BSU 05590
gene id: 224308:BSU_05590
uniprot id: A0A6M3Z7T8
uniprot id: P96702
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 04260
gene id: 224308:BSU_04260
uniprot id: A0A6M3Z855
uniprot id: P96583
names: Caution
names: Function
names: Similarity
messages: Belongs to the type IA topoisomerase family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

224308:BSU 32320
gene id: 224308:BSU_32320
uniprot id: O32128
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37540
gene id: 224308:BSU_37540
uniprot id: A0A6M3ZHJ8
uniprot id: P70994
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the 4-oxalocrotonate tautomerase family.
messages: Catalyzes both 1,3- and 1,5-keto-enol tautomerization of the diacid 2-hydroxymuconate (2-hydroxy-2,4-hexadienedioate) to produce 2-oxo-4-hexenedioate. This reaction is highly stereoselective and produces a mixture of stereoisomers, where the (3S)-isomer of 2-oxo-4-hexenedioate predominates. Also catalyzes the tautomerization of 2-hydroxymuconate to 2-oxo-3-hexenedioate, however this reaction is slower and occurs after the tautomerization of 2-hydroxymuconate to 2-oxo-4-hexenedioate. Using 2-hydroxy-2,4-pentadienoate, phenylenolpyruvate, (p-hydroxyphenyl)-enolpyruvate and 2-hydroxy-2,4-heptadiene-1,7-dioate, YwhB is a highly efficient 1,3-keto-enol tautomerase, but clearly not a 1,5-keto-enol tautomerase. Tautomerization of the two monoacids 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate produces a mixture of stereoisomers, where the (3R)-isomers predominate.
messages: Homohexamer.
messages: The mono- and diacids apparently bind in different orientations in the active site of YwhB, but the highly stereoselective nature of the YwhB reaction using a diacid suggests that the biological substrate for YwhB may be a diacid.

224308:BSU 37940
gene id: 224308:BSU_37940
uniprot id: A0A6M3ZIB4
uniprot id: P39618
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
messages: Cell membrane
messages: Membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 26610
gene id: 224308:BSU_26610
uniprot id: A0A6M3ZEE4
uniprot id: P54428
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0053 family.
messages: Cell membrane
messages: Membrane

224308:BSU 08360
gene id: 224308:BSU_08360
uniprot id: A0A6M4JHJ3
uniprot id: O31559
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the acyltransferase 3 family.
messages: Cell membrane

224308:BSU 09650
gene id: 224308:BSU_09650
uniprot id: A0A6M4JHV4
uniprot id: O07595
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the sirtuin family. Class U subfamily.
messages: Binds 1 zinc ion per subunit.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form.

224308:BSU 02810
gene id: 224308:BSU_02810
uniprot id: O34360
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M15C family.
messages: Cell membrane
messages: Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall.
messages: Expressed during vegetative growth phase.

224308:BSU 16180
gene id: 224308:BSU_16180
uniprot id: A0A6M3ZAT0
uniprot id: P24500
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Bacterial flagellum basal body
messages: Belongs to the flagella basal body rod proteins family.
messages: Lack flagella. Altered cell surface properties including greater softness and lower density of the charged groups in the polymer layer.
messages: Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.
messages: The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.
messages: The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod. In Gram-negative bacteria, at least four rings, L, P, S and M are present, whereas Gram-positive bacteria lack the L and P rings. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF build up the proximal portion of the rod with about 6 subunits each. Rod assembly occurs by export via the flagellum-specific pathway of its constituent proteins and by their incorporation into the rod structure in the probable order of FlgB, FlgC, FlgF and FlgG. Another protein, FliE, also assembles onto the stable rod structure.

224308:BSU 19530
gene id: 224308:BSU_19530
uniprot id: A0A6M3ZBZ0
uniprot id: O34882
names: Similarity
messages: Belongs to the 4-oxalocrotonate tautomerase family.

224308:BSU 24580
gene id: 224308:BSU_24580
uniprot id: P54509
names: Similarity
messages: Belongs to the SNF2/RAD54 helicase family.

224308:BSU 31750
gene id: 224308:BSU_31750
uniprot id: A0A6M4JP01
uniprot id: O32090
names: Function
names: PTM
names: Similarity
messages: Belongs to the NAPRTase family.
messages: Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.
messages: Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.
messages: Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.

224308:BSU 35860
gene id: 224308:BSU_35860
uniprot id: A0A6M3ZL68
uniprot id: P96740
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase C40 family.
messages: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA).
messages: In stationary phase; under control of SigD.
messages: Inhibited by pretreatment with 1 mM 4-(hydroxymercuri)benzoate, a sulfhydryl inhibitor.
messages: Secreted
messages: cell wall

224308:BSU 23170
gene id: 224308:BSU_23170
uniprot id: P35158
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SpmB family.
messages: Cell membrane
messages: Involved in spore core dehydration; might be involved in the transport of something into or out of the forespore or could be required for some modification of the cortex peptidoglycan structure.

224308:BSU 36170
gene id: 224308:BSU_36170
uniprot id: A0A6M4JPZ3
uniprot id: P96724
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the endonuclease V family.
messages: Cytoplasm
messages: DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.
messages: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon has no visible phenotype, however it is out-competed by wild-type cells.

224308:BSU 35960
gene id: 224308:BSU_35960
uniprot id: P36949
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 2 family.
messages: Cell membrane
messages: Membrane raft
messages: Part of the ABC transporter complex RbsABC involved in ribose import. Binds ribose.
messages: The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB) (By similarity). Interacts with FloT (PubMed:23651456).

224308:BSU 01180
gene id: 224308:BSU_01180
uniprot id: A0A6M3Z6Q4
uniprot id: P42924
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL23 family.
messages: One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Contacts protein L29, and trigger factor when it is bound to the ribosome (By similarity).
messages: Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.

224308:BSU 01320
gene id: 224308:BSU_01320
uniprot id: A0A6M3Z9V8
uniprot id: P46899
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL18 family.
messages: Part of the 50S ribosomal subunit (PubMed:30126986). Part of the 5S rRNA/L5/L18/L25 subcomplex (Probable). Contacts the 23S rRNA and 5S rRNA (Probable). Required for catalysis of RNase M5 (PubMed:6432797).
messages: Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.
messages: Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor, which is does in conjunction with ribonuclease M5 (RNase M5, rnmV). Possibly folds the 5S rRNA precursor into the correct conformation, thus acting as a chaperone.
messages: This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.

224308:BSU 01580
gene id: 224308:BSU_01580
uniprot id: A0A6M4JF66
uniprot id: P55189
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
messages: Cell membrane
messages: Membrane

224308:BSU 37930
gene id: 224308:BSU_37930
uniprot id: A0A6M4JQH9
uniprot id: P39619
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0382 family.
messages: Cell membrane
messages: Membrane
messages: Negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 24470
gene id: 224308:BSU_24470
uniprot id: A0A6M4JKN5
uniprot id: P54517
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the type-II 3-dehydroquinase family.
messages: Catalyzes a trans-dehydration via an enolate intermediate.
messages: Homododecamer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Phe-23 is present instead of the conserved Tyr which is expected to be an active site residue.

224308:BSU 29530
gene id: 224308:BSU_29530
uniprot id: A0A6M4JLY9
uniprot id: O34525
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Autocatalytically cleaves its own C-terminus.
messages: Belongs to the peptidase S49 family.
messages: Cell membrane
messages: Digestion of cleaved signal peptides (By similarity). Required for efficient processing of precursors under conditions of hyper-secretion. Has a preference for leucine-rich substrate peptides.
messages: Homooctamer, assembles into a ring structure with a central cavity (PubMed:22472423, PubMed:24228759). Interacts with FloT (PubMed:23651456).
messages: Membrane raft

224308:BSU 04960
gene id: 224308:BSU_04960
uniprot id: A0A6M4JD58
uniprot id: P96644
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane

224308:BSU 04440
gene id: 224308:BSU_04440
uniprot id: P96600
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the bacterial solute-binding protein 7 family.
messages: Part of the binding-protein-dependent transport system for uptake of C4-dicarboxylates. Responsible for growth on fumarate and succinate but not malate. Is not directly involved in C4-dicarboxylate uptake, but plays a sensory role in the DctS/DctR two-component system which regulates the expression of the dctA C4-dicarboxylate transporter.
messages: Secreted

224308:BSU 11640
gene id: 224308:BSU_11640
uniprot id: O31615
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.
messages: Binds cyclic di-AMP (c-di-AMP), which may regulate the transporter activity.
messages: Cell membrane
messages: Probable Na(+)/H(+) antiporter.

224308:BSU 02140
gene id: 224308:BSU_02140
uniprot id: A0A6M4JI19
uniprot id: P37948
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.
messages: Cell membrane
messages: Membrane
messages: Responsible for glycerol-3-phosphate uptake.

224308:BSU 03740
gene id: 224308:BSU_03740
uniprot id: A0A6M4JGA8
uniprot id: P94412
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC-4 integral membrane protein family.
messages: Cell membrane
messages: Membrane

224308:BSU 33040
gene id: 224308:BSU_33040
uniprot id: A0A6M4JP24
uniprot id: P07343
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
messages: Cytoplasm
messages: Homotetramer.
messages: Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.
messages: There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.

224308:BSU 31520
gene id: 224308:BSU_31520
uniprot id: A0A6M3ZG86
uniprot id: O05250
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of a two-component regulatory system MalK/MalR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates MalR by phosphorylation.
messages: Membrane

224308:BSU 24450
gene id: 224308:BSU_24450
uniprot id: A0A6M3ZDK4
uniprot id: P49778
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the elongation factor P family.
messages: Cytoplasm
messages: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase (By similarity).
messages: Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.
messages: No sporulation at 37 or 47 degrees Celsius.
messages: Plays a role in sporulation.

224308:BSU 16480
gene id: 224308:BSU_16480
uniprot id: P40405
names: Function
messages: Required for swarming motility and for maximal sigma-D activity.

224308:BSU 27610
gene id: 224308:BSU_27610
uniprot id: A0A6M4JLI1
uniprot id: O34443
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family.
messages: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 17050
gene id: 224308:BSU_17050
uniprot id: A0A6M3ZEJ0
uniprot id: P49850
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the DNA mismatch repair MutL/HexB family.
messages: Cells lacking this gene have an 80-fold increased spontaneous mutation frequency. A double mutS/mutL and a triple mutS/mutL/nth disruption has a 200 to 280-fold increased spontaneous mutation frequency.
messages: This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex (By similarity). Overexpression of mutSL partially suppresses the high spontaneous mutation frequency of a ytkD/mutM/mutY triple disruption which lacks the system required to prevent damage by oxidized guanine (8-oxo-dGTP). This suggests that MutSL also functions to repair mismatches due to oxidative stress in both growing and stationary phase cells.
messages: This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.

224308:BSU 05530
gene id: 224308:BSU_05530
uniprot id: P96696
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0702 family.
messages: Cell membrane

224308:BSU 11550
gene id: 224308:BSU_11550
uniprot id: A0A6M3Z9F1
uniprot id: O31606
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Adapter protein required for efficient degradation of Spx by ClpXP under non-stress conditions (PubMed:17908206, PubMed:19074380, PubMed:24942655). Interaction with Spx stabilizes Spx and exposes the C-terminus of Spx for recognition and proteolysis by ClpXP (PubMed:24942655, PubMed:27191337). Is specific for Spx and does not enhance proteolysis by ClpCP protease (PubMed:19074380). Probably binds 2 zinc ions (PubMed:19074380).
messages: Adapter protein required for efficient degradation of Spx by ClpXP under non-stress conditions. Interaction with Spx stabilizes Spx and exposes the C-terminus of Spx for recognition and proteolysis by ClpXP.
messages: Belongs to the SpxH family.
messages: Cytoplasm
messages: Expressed throughout cell growth. Negatively influences its own expression.
messages: Interacts with Spx (PubMed:19074380, PubMed:24942655, PubMed:27191337). Interacts with SpxO/YuzO (PubMed:21378193).
messages: Interacts with Spx.
messages: Irreversible aggregation upon several stress conditions prevents interaction with Spx and therefore leads to Spx stabilization (PubMed:25353645). Inhibited by interaction with SpxO/YuzO (PubMed:21378193).
messages: Strains lacking this gene show poor growth, affected sporulation, complete loss of competence development and overproduce Spx under unperturbed growth. Mutants also overproduce TrxA and show reduced sensitivity to disulfide stress.
messages: The histidine-rich N terminus is essential for interaction with Spx.

224308:BSU 28950
gene id: 224308:BSU_28950
uniprot id: A0A6M3ZEW6
uniprot id: P18255
names: Caution
names: Cofactor
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).
messages: Cytoplasm
messages: Expressed during vegetative growth.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 27710
gene id: 224308:BSU_27710
uniprot id: A0A6M4JM24
uniprot id: O32053
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the queuine tRNA-ribosyltransferase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
messages: Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

224308:BSU 07550
gene id: 224308:BSU_07550
uniprot id: P80241
names: Induction
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.

224308:BSU 04860
gene id: 224308:BSU_04860
uniprot id: P96634
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 10350
gene id: 224308:BSU_10350
uniprot id: A0A6M4JH19
uniprot id: O07618
names: Function
names: Induction
names: Similarity
messages: Belongs to the thiolase-like superfamily. Thiolase family.
messages: May be involved in fatty acid metabolism.
messages: Repressed by presence of biotin, under control of BirA. Probably part of the bioY-yhfST operon.

224308:BSU 37790
gene id: 224308:BSU_37790
uniprot id: A0A6M3ZHJ1
uniprot id: P39633
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
messages: Cells lacking this gene lose the ability to utilize proline, ornithine, or arginine as sole carbon source and grow more slowly when proline or ornithine is utilized as sole nitrogen source in the presence of glucose. A double rocG-gudB disruption has the same phenotype as a single rocG disruption.
messages: Devoted to catabolic function of glutamate (and other amino acids of the glutamate family) utilization as sole nitrogen source. It is not involved in anabolic function of glutamate biosynthesis since B.subtilis possesses only one route of glutamate biosynthesis from ammonia, catalyzed by glutamate synthase. Wild-type cells are unable to utilize glutamate or glutamine as a sole carbon source; thus RocG does not function physiologically to synthesize glutamate, but it is involved in the utilization of arginine, and proline as carbon or nitrogen source (PubMed:9829940). The catabolic RocG is essential for controlling gltAB expression via an inhibitory interactions with the transcriptional regulator GltC in response to the availability of sugars (PubMed:17183217, PubMed:15150224).
messages: Enzyme activity is low in early exponential phase and reached higher levels in the middle and late stages of exponential growth (at protein level). Repressed by glucose; induced by arginine, ornithine, or to a lesser extent proline (in the absence of glucose). Not induced by glutamate or glutamine (PubMed:9829940). Expression depends on the alternative sigma-L factor and the transcription factor RocR (PubMed:10468601). Subject to direct CcpA-dependent glucose repression (PubMed:15150224).
messages: Homohexamer (PubMed:16244435, PubMed:20630473). Interacts with transcriptional regulator GltC (PubMed:17608797).

224308:BSU 17330
gene id: 224308:BSU_17330
uniprot id: A0A6M4JKB0
uniprot id: O31795
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the IPP transferase family.
messages: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Monomer.

224308:BSU 00060
gene id: 224308:BSU_00060
uniprot id: A0A6M3ZII2
uniprot id: P05652
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
messages: Belongs to the type II topoisomerase GyrB family.
messages: Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).
messages: Cytoplasm
messages: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
messages: Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

224308:BSU 03200
gene id: 224308:BSU_03200
uniprot id: A0A6M4JRC4
uniprot id: P94390
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the proline dehydrogenase family.
messages: Binds 1 FAD per subunit.
messages: Converts proline to delta-1-pyrroline-5-carboxylate. Important for the use of proline as a sole carbon and energy source or a sole nitrogen source.
messages: Deletion of the putBCP operon abolishes L-proline utilization.
messages: The expression of the putBCP operon is induced in a PutR-dependent fashion by very low concentrations of L-proline in the growth medium. CodY represses the operon by displacing PutR from DNA.

224308:BSU 40680
gene id: 224308:BSU_40680
uniprot id: P37500
names: Function
names: Similarity
messages: Belongs to the UPF0039 (ElaA) family.
messages: Could catalyze the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to an acceptor substrate and release both CoA and the acetylated product.

224308:BSU 36830
gene id: 224308:BSU_36830
uniprot id: A0A6M4JQ33
uniprot id: P37808
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ATPase alpha/beta chains family.
messages: Cell membrane
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains (Probable). The F(1)F(0) complex interacts with SpoIIIJ and YqjG; YqgA is found in the same complex.
messages: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.
messages: Membrane raft
messages: Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.

224308:BSU 03720
gene id: 224308:BSU_03720
uniprot id: A0A6M3ZAJ4
uniprot id: P49940
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
messages: Cell membrane
messages: Involved in the germination response to the combination of glucose, fructose, L-asparagine, and KCl.
messages: Membrane

224308:BSU 02790
gene id: 224308:BSU_02790
uniprot id: O34621
names: Similarity
messages: To B.subtilis YcdC.

224308:BSU 33900
gene id: 224308:BSU_33900
uniprot id: A0A6M4JPA4
uniprot id: P37869
names: Activity Regulation
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the enolase family.
messages: Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
messages: Cell surface
messages: Cytoplasm
messages: Homooctamer. Component of a possible RNA degradosome complex composed of rny, rnjA, rnjB, pnp, pfkA and eno (PubMed:19193632) (although rnjA and rnjB's presence is unclear).
messages: Phosphorylated during sporulation.
messages: Secreted
messages: The covalent binding to the substrate at Lys-339 of a small fraction of enolase causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein (PubMed:15003462). Citrate acts as a non-competitive inhibitor for both forward and reverse reactions, probably by chelating Mg(2+) (PubMed:22198292).
messages: The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.

224308:BSU 31360
gene id: 224308:BSU_31360
uniprot id: O05240
names: Similarity
messages: Belongs to the iron-containing alcohol dehydrogenase family.

224308:BSU 37810
gene id: 224308:BSU_37810
uniprot id: Q7WY56
names: Similarity
messages: To dTDP-4-dehydrorhamnose reductase.

224308:BSU 37030
gene id: 224308:BSU_37030
uniprot id: A0A6M4JQ73
uniprot id: P45870
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RacA family.
messages: Cytoplasm
messages: Expressed at the early stages of sporulation.
messages: Required for the formation of axial filaments and for anchoring the origin regions at the cell poles in sporulating cells, thus ensuring proper chromosome segregation in the prespore. Binds in a dispersed manner throughout the chromosome but preferentially to sites clustered in the origin portion of the chromosome, causing condensation of the chromosome and its remodeling into an elongated, anchored structure.

224308:BSU 05610
gene id: 224308:BSU_05610
uniprot id: A0A6M4JDR2
uniprot id: P39115
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. ABCF family. ARE2 subfamily.
messages: Binds within the E-site of the 70S ribosome, where it contacts ribosomal proteins L1, L5, L33-1, S7, S11, the 16 and 23S rRNAs and the acceptor arm of the P-site tRNA.
messages: Binds within the E-site of the 70S ribosome, where it contacts ribosomal proteins of the large and small subunit, the 16 and 23S rRNAs and the acceptor arm of the P-site tRNA.
messages: Cytoplasm
messages: Expressed during all growth phases; expression is higher during early log phase and decreases as growth continues. Expression is dramatically increased in the presence of antibiotics virginiamycin M and lincomycin. A monocistronic operon.
messages: Hypersensitivity toward the antibiotics virginiamycin M and lincomycin, mildly increased sensitivity to erythromycin, clindamycin and oleandomycin (PubMed:16109936). Hypersensitivity toward the antibiotics virginiamycin M, lincomycin and tiamulin, in this study has no effect on sensitivity to erythromycin, chloramphenicol or linezolid (PubMed:30126986).
messages: Recognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site.
messages: Recognizes and binds in the vacant E-site of ribosomes stalled by some peptidyltransferase center (PTC)-targeting antibiotics. Makes contact with the PTC and both ribosomal subunits. Induces conformational changes in the P-site, which allows it to dislodge the antibiotic from its PTC binding site. Binds to ribosomes either directly following translation initation or subsequent to E tRNA release during elongation (PubMed:30126986). Involved in resistance to a narrow spectrum of antibiotics (the streptogramin A antibiotic virginiamycin M, the lincosamide antibiotic lincomycin and the pleuromutilin antibiotic tiamulin) (PubMed:16109936, PubMed:30126986).
messages: The antibiotic resistance domain (ARD) is packed between the 23S rRNA and the acceptor arm of the P-site tRNA and inserts into the peptidyltransferase center (PTC). The C-terminal extension (CTE) contacts the small ribosomal subunit, positioned in the Shine-Dalgarno-anti-Shine-Dalgarno cavity.

224308:BSU 33240
gene id: 224308:BSU_33240
uniprot id: A0A6M3ZFZ5
uniprot id: O34714
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Binds 2 manganese ions per subunit.
messages: Converts oxalate to formate and CO(2) in an O(2)-dependent reaction. Can also catalyze minor side reactions: oxalate oxidation to produce H(2)O(2), and oxalate-dependent, H(2)O(2)-independent dye oxidations.
messages: Cytoplasm
messages: Homohexamer.
messages: Induced by acid pH but not by oxalate (PubMed:10960116). Positively regulated by SigO and its coactivator RsoA (PubMed:18573182).
messages: To B.subtilis OxdD.

224308:BSU 08800
gene id: 224308:BSU_08800
uniprot id: A0A6M4JLC2
uniprot id: Q796Y5
names: Similarity
messages: Belongs to the oxygen-dependent FAD-linked oxidoreductase family.

224308:BSU 15850
gene id: 224308:BSU_15850
uniprot id: A0A6M3ZAP2
uniprot id: O34635
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the iron-sulfur dependent L-serine dehydratase family.
messages: Binds 1 [4Fe-4S] cluster.
messages: Heterodimer of an alpha chain and a beta chain.

224308:BSU 15640
gene id: 224308:BSU_15640
uniprot id: A0A6M4JI41
uniprot id: O34693
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Activity of this protein is best tested in a clpP or ssrA deletion mutant; ssrA (tmRNA) encodes the SsrA tag added to nascent proteins in stalled ribosomes by trans-translation, which targets the nascent protein for degradation.
messages: Associates with stalled 50S ribosomal subunits.
messages: Belongs to the NEMF family.
messages: Highly expressed during stationary phase.
messages: Not essential in rich media; synthetic growth defects are seen in double rqcH ssrA mutants, further exacerbated by translational inhibitors spectinomycin and erythromycin or at elevated temperatures. Decreased accumulation of a stalled reporter protein. Triple rqcH clpP ssrA mutants cannot be generated.
messages: Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. May add between 1 and at least 8 Ala residues. Binds to stalled 50S ribosomal subunits.
messages: Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. Selectively binds tRNA(Ala)(UGC), which is presumably the sole source of tRNA(Ala) used for Ala tailing directed by this protein. May add between 1 and at least 8 Ala residues; detection of the Ala tail requires either deletion of clpP or its inhibition. Binds to 50S ribosomal subunits, at least 30% of which contain a P-site tRNA and thus are obstructed.
messages: Up-regulated under secretion stress conditions, possibly in a CssR/CssS-dependent manner.

224308:BSU 35010
gene id: 224308:BSU_35010
uniprot id: A0A6M4JPI8
uniprot id: O34450
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the metallo-dependent hydrolases superfamily. NagA family.
messages: Binds 1 divalent metal cation per subunit.
messages: Binds 2 divalent metal cations per subunit.
messages: Expression is repressed by the HTH-type transcriptional regulator NagR.
messages: Homodimer.
messages: Involved in the first committed step in the biosynthesis of amino-sugar-nucleotides. Catalyzes the hydrolysis of the N-acetyl group of N-acetylglucosamine-6-phosphate (GlcNAc-6-P) to yield glucosamine 6-phosphate and acetate.

224308:BSU 04480
gene id: 224308:BSU_04480
uniprot id: A0A6M3ZCW1
uniprot id: P96604
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.
messages: Cell membrane
messages: Membrane

224308:BSU 10430
gene id: 224308:BSU_10430
uniprot id: P40398
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 24040
gene id: 224308:BSU_24040
uniprot id: P37941
names: Function
names: Subunit
messages: Heterotetramer of two alpha and two beta chains. Directly associated with ODBA in the E1 complex.
messages: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

224308:BSU 00850
gene id: 224308:BSU_00850
uniprot id: A0A6M4JEL5
uniprot id: P37570
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Appears to be allosterically activated by the binding of pArg-containing polypeptides to the pArg-binding pocket localized in the C-terminal domain of McsB (By similarity). The McsB kinase is inhibited in nonstressed cells by direct interaction with ClpC; upon heat exposure, the interaction of McsB with ClpC is dramatically decreased, leading to McsB release and activation during heat stress. Its kinase activity is counteracted by the protein-arginine-phosphatase YwlE in vivo. Requires McsA for full kinase activity.
messages: Appears to be allosterically activated by the binding of pArg-containing polypeptides to the pArg-binding pocket localized in the C-terminal domain of McsB.
messages: Autophosphorylated on Arg residues. Phosphorylation on Arg-40 and Arg-86 are up-regulated upon stress conditions.
messages: Belongs to the ATP:guanido phosphotransferase family.
messages: Catalyzes the specific phosphorylation of arginine residues in a large number of proteins. Is part of the bacterial stress response system, where it is involved in regulating the global heat shock repressor CtsR; phosphorylates arginine residues in the winged helix-turn-helix domain of CtsR, thereby preventing its binding to DNA and consequently inducing the expression of repressed genes. The transcriptional repressor HrcA, the chaperone GroEL, the unfoldase ClpC, together with several ribosomal subunits, represent other physiological targets of McsB under stress conditions. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity. Functions as an adapter whose kinase activity is required for ClpCP-mediated degradation of CtsR during heat stress. Is required for the delocalization of competence proteins from the cell poles, probably via a role in the degradation of anchor proteins.
messages: Catalyzes the specific phosphorylation of arginine residues in a large number of proteins. Is part of the bacterial stress response system. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity.
messages: Cells lacking this gene show a defect in the delocalization of competence proteins that is not related to altered expression of the com genes on the levels of either transcription or translation. Inactivation of mcsB also decreases transformability (PubMed:19226326). CtsR is no more degraded (PubMed:30962626).
messages: Cytoplasm
messages: Interacts with CtsR in its autophosphorylated form. Interacts with McsA in nonstressed as well as in heat-stressed cells, whereas strongly interacts with ClpC only in nonstressed cells.
messages: Is repressed by the transcriptional regulator CtsR. Forms part of an operon with ctsR, mcsA and clpC.

224308:BSU 14200
gene id: 224308:BSU_14200
uniprot id: A0A6M3ZAK4
uniprot id: O34783
names: Similarity
messages: Belongs to the UPF0180 family.

224308:BSU 34370
gene id: 224308:BSU_34370
uniprot id: A0A6M3ZG81
uniprot id: P71050
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CpsC/CapA family.
messages: Cell membrane
messages: Membrane

224308:BSU 12220
gene id: 224308:BSU_12220
uniprot id: O34539
names: Activity Regulation
names: Domain
names: Function
names: Similarity
names: Subunit
messages: Activity is improved in the presence of Mn(2+), Mg(2+) and Ca(2+), and inhibited by Ni(2+), Zn(2+) and Cu(2+).
messages: Adopts the classical GT-B fold containing the N-terminal and C-terminal domains that accommodate the sugar acceptor and UDP-glucose, respectively (PubMed:33310191, PubMed:33152360). The spacious sugar-acceptor binding pocket might be responsible for the broad substrate spectrum (PubMed:33310191).
messages: Belongs to the UDP-glycosyltransferase family.
messages: Glycosyltransferase that can glycosylate a wide range of substrates, including various flavonoids, phenyl ketones, curcuminoid, lignins, zingerone, triterpenes, stilbene and anthraquinone, using UDP-glucose or ADP-glucose as sugar donor (PubMed:28315700, PubMed:33152360). It also exhibits O-, N- and S-glycosylation activities towards simple aromatics (PubMed:28315700). In vivo, the broad acceptor tolerance of YjiC might function as a detoxification agent against exogenous xenobiotics to make the strain adaptable to the changeable environment (Probable).
messages: Monomer.

224308:BSU 16270
gene id: 224308:BSU_16270
uniprot id: A0A6M4JIN9
uniprot id: P23451
names: Function
names: Similarity
messages: Belongs to the FliK family.
messages: Controls the length of the flagellar hook.

224308:BSU 03270
gene id: 224308:BSU_03270
uniprot id: A0A6M3Z7R4
uniprot id: O31475
names: Caution
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the ferredoxin--NADP reductase type 2 family.
messages: Binds 1 FAD per subunit.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 29680
gene id: 224308:BSU_29680
uniprot id: P39062
names: Disruption Phenotype
names: Function
names: PTM
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme (By similarity).
messages: Belongs to the ATP-dependent AMP-binding enzyme family.
messages: Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA (By similarity). Has a role in growth and sporulation on acetate.
messages: Cell membrane
messages: Disruption of this gene induces a loss of the ability to utilize acetate as a carbon source for growth or sporulation.
messages: Interacts with FloT.
messages: Membrane raft

224308:BSU 37190
gene id: 224308:BSU_37190
uniprot id: P45865
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phospholipase D family. Cardiolipin synthase subfamily.
messages: Cell membrane
messages: Cells lacking this gene show a lack of cardiolipin.
messages: Involved in the biosynthesis of cardiolipin.

224308:BSU 16520
gene id: 224308:BSU_16520
uniprot id: A0A6M4JGF1
uniprot id: P81101
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the RRF family.
messages: Cytoplasm
messages: No sporulation at 37 or 47 degrees Celsius.
messages: Plays a role in sporulation.
messages: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.

224308:BSU 25290
gene id: 224308:BSU_25290
uniprot id: A0A6M4JLW8
uniprot id: P42182
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity). Binds both GDP and GTP. Complements an E.coli era disruption mutant.
messages: An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.
messages: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.
messages: Cell membrane
messages: Constitutively expressed, two-fold induced at the end of the exponential phase; this is under control of Spo0A, suggesting it may have a role in sporulation.
messages: Cytoplasm
messages: Essential in some but not all strains; in 168 / BR151 the null mutation grows slowly and forms irregularly shaped colonies after several days. In liquid culture forms chains of elongated cells with diffuse nucleoids that occupy most of the cell. Sporulation in this disruption strain is severely impaired. Essential in strains CRK6000 and IS75, where it cannot be disrupted. In CRK600 in depletion experiments cells become 1.5 to 2-fold longer and nucleoid distribution is dispersed. The number of replication origins increases, suggesting an increase in chromosome replication.
messages: Estimated to be present at 3000 copies per cell.
messages: Monomer.

224308:BSU 18250
gene id: 224308:BSU_18250
uniprot id: O31826
names: Similarity
messages: Belongs to the ATP-dependent AMP-binding enzyme family.

224308:BSU 37140
gene id: 224308:BSU_37140
uniprot id: P06629
names: Caution
messages: Was originally thought to be the spo0F protein.

224308:BSU 14810
gene id: 224308:BSU_14810
uniprot id: O07635
names: Similarity
messages: In the C-terminal section; belongs to the PhoH family.

224308:BSU 23490
gene id: 224308:BSU_23490
uniprot id: A0A6M3ZDN2
uniprot id: P46354
names: Function
names: Similarity
names: Subunit
messages: Belongs to the PNP/MTAP phosphorylase family.
messages: Homotrimer.
messages: The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
messages: The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine, inosine, 2'-deoxyguanosine and 2'-deoxyinosine.

224308:BSU 19800
gene id: 224308:BSU_19800
uniprot id: P42094
names: Subcellular Location Annotation
messages: Secreted

224308:BSU 06070
gene id: 224308:BSU_06070
uniprot id: A0A6M4JG30
uniprot id: O34680
names: Activity Regulation
names: Caution
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: A methylase, recognizes the double-stranded sequence 5'-YTCGAR-3', methylates C-3 on both strands, and protects the DNA from cleavage by the BsuMI endonuclease.
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
messages: Constitutively expressed during exponential growth. Encoded in an operon with ydiO and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible.
messages: Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025, YdjA, YdiS and YdiR respectively) has been disrupted (PubMed:11751814). Triple deletions ydiO-ydiP-ydiR, ydiO-ydiP-ydiS and ydiO-ydiP-ydjA lead to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).
messages: Monomer (PubMed:3150363). May form a complex with YdiP, also seems to be active alone (Probable).
messages: Not expressed during sporulation.
messages: Somewhat inhibited by MgCl(2) and spermidine, strongly inhibited by MnCl(2).
messages: The characterized enzyme was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.

224308:BSU 09070
gene id: 224308:BSU_09070
uniprot id: P54591
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 01020
gene id: 224308:BSU_01020
uniprot id: A0A6M3Z9T5
uniprot id: Q06796
names: Function
names: Miscellaneous
names: PTM
names: Similarity
names: Subunit
messages: An unidentified mutation in this gene gives rise to thiostrepton resistance.
messages: Belongs to the universal ribosomal protein uL11 family.
messages: Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.
messages: One or more lysine residues are methylated.
messages: Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which 2 L12 dimers bind in a sequential fashion forming a pentameric L10(L12)2(L12)2 complex.
messages: Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.

224308:BSU 27980
gene id: 224308:BSU_27980
uniprot id: P26936
names: Developmental Stage
names: Function
names: PTM
names: Subcellular Location Annotation
names: Subunit
messages: Forespore outer membrane
messages: Forms a complex with BofA and SpoIVFB localized in the mother-cell membrane surrounding the forespore.
messages: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation at 37 degrees Celsius, but not at 30 degrees Celsius. SpoIVFA plays a central role in both maintaining the SpoIVFA/BofA/SpoIVFB complex and anchoring it to the outer forespore membrane. SpoIVFA brings BofA into close proximity to SpoIVFB, allowing BofA to inhibit SpoIVFB. Increased accumulation of SpoIVFA seems to inhibit the activity of SpoIVFB and thus regulates the activation of sigma-K.
messages: May be degraded by FtsH. It is stabilized by an ftsH disruption mutant, and in a probably independent fashion, by overexpression of BofA.
messages: Transcribed during the stage II, but not required until stage IV of sporulation.

224308:BSU 29830
gene id: 224308:BSU_29830
uniprot id: A0A6M3ZFA7
uniprot id: O34496
names: Similarity
messages: Belongs to the UPF0354 family.

224308:BSU 07760
gene id: 224308:BSU_07760
uniprot id: A0A6M3Z910
uniprot id: O34573
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
messages: Cell membrane
messages: May be involved in spore germination.
messages: Membrane

224308:BSU 07930
gene id: 224308:BSU_07930
uniprot id: O34579
names: Induction
messages: Transcriptionally regulated by the forespore-specific sigma factor, SigG, and the general stress response regulator, SigB.

224308:BSU 18020
gene id: 224308:BSU_18020
uniprot id: A0A6M4JH59
uniprot id: Q7WY69
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspN family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Expression is sigma F and sigma G-dependent.
messages: Spore core

224308:BSU 23560
gene id: 224308:BSU_23560
uniprot id: A0A6M4JL22
uniprot id: P54571
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter family.
messages: Cell membrane
messages: Couples proton uptake and Na(+) efflux to the substrate-product malate/lactate antiport, in an electroneutral malate-2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force.
messages: Membrane

224308:BSU 21340
gene id: 224308:BSU_21340
uniprot id: O31975
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 34320
gene id: 224308:BSU_34320
uniprot id: A0A6M3ZGV2
uniprot id: P71055
names: Function
names: Induction
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance.
messages: Repressed by SinR.

224308:BSU 19040
gene id: 224308:BSU_19040
uniprot id: P37584
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Can suppress growth and secretion defects in E.coli secA and secB mutants (PubMed:1435734). Probably a molecular chaperone for exported proteins or may act by stabilizing the SecA protein (PubMed:1435734, PubMed:10816431).
messages: Cytoplasm
messages: Grows normally, reduced secretion of SdpC (formerly YvaY) and another 36 kDa protein.
messages: Homodimer (PubMed:17372352). Probably binds SecA and prePhoB (PubMed:10816431). Probably interacts with proSdpC (PubMed:13129613).
messages: Present in exponentially growing cells (at protein level).

224308:BSU 21730
gene id: 224308:BSU_21730
uniprot id: P54179
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 25250
gene id: 224308:BSU_25250
uniprot id: O34994
names: Disruption Phenotype
names: Function
names: Induction
messages: Constitutively expressed.
messages: Relieves catabolite repression of gapB and pckA transcription. Disruption is epistatic over a yqfL deletion.
messages: Transcription repressor that binds to the promoter of gapB and pckA genes, preventing their expression. Acts as a regulator for catabolite repression of gluconeogenic genes.

224308:BSU 29150
gene id: 224308:BSU_29150
uniprot id: A0A6M4JKP8
uniprot id: O34811
names: Caution
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0756 family.
messages: Cell membrane
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 32510
gene id: 224308:BSU_32510
uniprot id: O32147
names: Function
names: Induction
names: Subunit
messages: Could be composed of four subunits: PucA, PucC, PucD and PucE.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
messages: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function.

224308:BSU 12700
gene id: 224308:BSU_12700
uniprot id: P54336
names: Similarity
messages: To B.subtilis YqbQ.

224308:BSU 30540
gene id: 224308:BSU_30540
uniprot id: P54420
names: Function
names: Similarity
messages: Belongs to the asparagine synthetase family.
messages: Main asparagine synthetase in vegetative cells.

224308:BSU 09790
gene id: 224308:BSU_09790
uniprot id: A0A6M3Z8Z7
uniprot id: O07543
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0754 family.
messages: Cell membrane

224308:BSU 27410
gene id: 224308:BSU_27410
uniprot id: A0A6M4JMA0
uniprot id: O34526
names: Cofactor
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Binds 1 zinc ion per subunit.
messages: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.
messages: Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
messages: Cytoplasm

224308:BSU 05180
gene id: 224308:BSU_05180
uniprot id: A0A6M3Z8D7
uniprot id: P96663
names: Similarity
messages: In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 17060
gene id: 224308:BSU_17060
uniprot id: Q7WY71
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 06930
gene id: 224308:BSU_06930
uniprot id: O31514
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37490
gene id: 224308:BSU_37490
uniprot id: A0A6M3ZK40
uniprot id: P70999
names: Function
names: Similarity
messages: Belongs to the arginase family. Agmatinase subfamily.
messages: Catalyzes the formation of putrescine from agmatine.

224308:BSU 22310
gene id: 224308:BSU_22310
uniprot id: A0A6M4JL55
uniprot id: P39792
names: Caution
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RecU family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Cells lacking this gene have a greatly increased sensitivity to DNA damaging agents, 25-fold decreased plasmid transformation and only slightly reduced chromosomal DNA transformation (PubMed:10692371). They form anucleate cells 100-times more frequently than wild-type cells during normal growth (PubMed:18684995). DNA damaging agent sensitivity is partially suppressed by disruption of radA and fin (also called sms and subA respectively) (PubMed:11810266).
messages: Cytoplasm
messages: Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation.
messages: Has at least 2 separable functions; Holliday junction resolution with generation of monomeric chromosomes, and modulation of RecA activity. Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation. Partially inhibits the hydrolysis of dATP or rATP by RecA. Holliday junction resolution is stimulated by RuvB.
messages: Homodimer. Interacts with RuvB.
messages: Recombination protein U, a short form proposed to initiate from Met-33, which is equivalent to RecU-delta1-32, is not detected in vivo, and would not be functional. The name is misleading and should no longer be used.
messages: Was originally named prfA (Penicillin-binding protein-related factor A, PBP-related factor A) due to its proximity to the ponA gene; this name is misleading and should no longer be used.

224308:BSU 09010
gene id: 224308:BSU_09010
uniprot id: A0A6M3Z8V5
uniprot id: P54585
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Membrane

224308:BSU 18560
gene id: 224308:BSU_18560
uniprot id: A0A6M3ZBD1
uniprot id: O34815
names: Induction
names: Similarity
messages: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
messages: Induced by sulfate, part of the yoaDCB operon.

224308:BSU 26160
gene id: 224308:BSU_26160
uniprot id: P45919
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 28850
gene id: 224308:BSU_28850
uniprot id: A0A6M4JNN9
uniprot id: P55873
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL20 family.
messages: Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity).
messages: Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.
messages: Part of the 50S ribosomal subunit.

224308:BSU 38190
gene id: 224308:BSU_38190
uniprot id: A0A6M3ZHA0
uniprot id: P39575
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the galactose-1-phosphate uridylyltransferase type 2 family.
messages: Cytoplasm

224308:BSU 10170
gene id: 224308:BSU_10170
uniprot id: A0A6M3ZCI3
uniprot id: O07600
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the thiolase-like superfamily. FabH family.
messages: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Has some substrate specificity for branched chain acyl-CoA, determining the biosynthesis of branched-chain of fatty acids instead of straight-chain.
messages: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.
messages: Cytoplasm
messages: Down-regulated by FapR.
messages: Homodimer.
messages: The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.

224308:BSU 04460
gene id: 224308:BSU_04460
uniprot id: A0A6M4JDH5
uniprot id: P96602
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system DctS/DctR. Essential for expression of dctP.
messages: Phosphorylated by DctS.

224308:BSU 11120
gene id: 224308:BSU_11120
uniprot id: A0A6M3ZEM6
uniprot id: P39803
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 02660
gene id: 224308:BSU_02660
uniprot id: A0A6M3Z723
uniprot id: P42253
names: Similarity
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

224308:BSU 29840
gene id: 224308:BSU_29840
uniprot id: O34357
names: Induction
messages: Up-regulated in response to depletion of tryptophan, phenylalanine, tyrosine, or availability of charged tRNATrp.

224308:BSU 33970
gene id: 224308:BSU_33970
uniprot id: P96711
names: Activity Regulation
names: Function
names: Subcellular Location Annotation
messages: Binding to DNA is inhibited by L-arabinose.
messages: Cytoplasm
messages: Transcriptional repressor of the arabinose utilization genes. Also regulates its own expression. Binds to two sequences within the promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one sequence in the araR promoter.

224308:BSU 37240
gene id: 224308:BSU_37240
uniprot id: A0A6M4JQ59
uniprot id: P45860
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the phospholipase D family. Cardiolipin synthase subfamily.
messages: By heat shock.
messages: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol (By similarity). May have a role in the heat shock response since the level of the transcript of ywiE increases after a heat shock.
messages: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol.
messages: Cell membrane
messages: Membrane

224308:BSU 27270
gene id: 224308:BSU_27270
uniprot id: A0A6M4JNK4
uniprot id: O32028
names: Function
names: Similarity
messages: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.
messages: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively (PubMed:10574451). Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine (By similarity).
messages: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine.

224308:BSU 08530
gene id: 224308:BSU_08530
uniprot id: O31576
names: Subunit
messages: Homodimer.

224308:BSU 21610
gene id: 224308:BSU_21610
uniprot id: O32001
names: Activity Regulation
names: Cofactor
names: Disruption Phenotype
names: Function
names: Subcellular Location Annotation
messages: Binds 1 Ca(2+) ion per subunit. Can also use Co(2+) or Mn(2+).
messages: Catalyzes the hydrolysis of supercoiled double and single strand DNA and RNA. Involved in chromosomal DNA degradation and cell death caused by thermal stress.
messages: Cell membrane
messages: Cells lacking this gene are more sensitive to mitomycin C, show an increased ability of competence and are not able to metabolize extracellular DNA.
messages: Inhibited by aurintricalboxylic acid but not by Zn(2+), Mn(2+), Hg(2+), 2-mercaptoethanol and sodium citrate. Neither inhibited nor activated by ATP.

224308:BSU 29000
gene id: 224308:BSU_29000
uniprot id: A0A6M3ZF23
uniprot id: Q45549
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the NrdR family.
messages: Binds 1 zinc ion.
messages: Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.

224308:BSU 07330
gene id: 224308:BSU_07330
uniprot id: O06480
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. YjjG family.

224308:BSU 22670
gene id: 224308:BSU_22670
uniprot id: A3F3D0
uniprot id: P03947
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the anthranilate phosphoribosyltransferase family.
messages: Binds 2 magnesium ions per monomer.
messages: Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 39500
gene id: 224308:BSU_39500
uniprot id: A0A6M3ZKF2
uniprot id: P54952
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 3 family.
messages: Cell membrane
messages: Membrane
messages: Membrane raft
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Probably part of the ABC transporter complex YxeMNO that could be involved in amino-acid import. May transport S-methylcysteine.
messages: The complex is composed of two ATP-binding proteins (YxeO), two transmembrane proteins (YxeN) and a solute-binding protein (YxeM).

224308:BSU 00550
gene id: 224308:BSU_00550
uniprot id: A0A6M4JE64
uniprot id: P37474
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.
messages: Cytoplasm
messages: In the C-terminal section; belongs to the helicase family. RecG subfamily.
messages: In the N-terminal section; belongs to the UvrB family.

224308:BSU 21630
gene id: 224308:BSU_21630
uniprot id: A0A6M3ZID7
uniprot id: O32003
names: Function
names: Similarity
names: Subunit
messages: Belongs to the antibiotic N-acetyltransferase family.
messages: Homodimer.
messages: May contribute to antibiotic resistance.

224308:BSU 02620
gene id: 224308:BSU_02620
uniprot id: P42251
names: Induction
names: PTM
names: Similarity
messages: Belongs to the PhoD family.
messages: By phosphate starvation.
messages: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

224308:BSU 06250
gene id: 224308:BSU_06250
uniprot id: P40775
names: Induction
messages: Positively regulated by the two-component system YycFG. Expression is maximal during exponential growth.

224308:BSU 05120
gene id: 224308:BSU_05120
uniprot id: A0A6M4JDM4
uniprot id: P39158
names: Induction
names: Subcellular Location Annotation
messages: Cytoplasm
messages: In response to low temperature.

224308:BSU 24810
gene id: 224308:BSU_24810
uniprot id: A0A6M3ZE17
uniprot id: P54499
names: Similarity
messages: Belongs to the UPF0045 family.

224308:BSU 20950
gene id: 224308:BSU_20950
uniprot id: O31936
names: Function
names: Subunit
messages: Forms a complex with cognate toxin YopC.
messages: May be the antitoxin component of a type II toxin-antitoxin (TA) system. Neutralizes the activity of cognate toxin YopC.

224308:BSU 17450
gene id: 224308:BSU_17450
uniprot id: P37582
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Subunit
messages: Homodimer under conditions of nitrogen excess (PubMed:1677938, PubMed:18331450, PubMed:25691471). Monomer under conditions of nitrogen-limited (PubMed:25691471). Interacts with feedback-inhibited GlnA in order to stabilizes GlnR-DNA complex (PubMed:1677938, PubMed:18331450, PubMed:25691471).
messages: In vivo, this mutant constitutively represses glnR expression. In vitro, this mutant binds DNA more tightly than wild-type and this binding is not activated by feedback-inhibited GlnA.
messages: The amino acid sequences of the N-terminal DNA binding domains of TnrA and GlnR are highly similar, and both proteins bind to DNA sequences with a common consensus sequence. In contrast, the C-terminal signal transduction domains of TnrA and GlnR have no homology.
messages: Transcription repressor that represses many genes including ureABC and tnrA, during nitrogen excess (PubMed:2573733, PubMed:1677938, PubMed:10231480, PubMed:18331450). On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, GlnR requires a DNA sequence of 17 nucleotides as minimal binding site (PubMed:10231480, PubMed:25691471).
messages: Under conditions of nitrogen excess, the DNA binding activity of GlnR is activated by a transient interaction with feedback-inhibited GlnA (PubMed:18331450). Under conditions of nitrogen-limited, GlnR is autoinhibited by its C-terminal region (PubMed:25691471).
messages: Under conditions of nitrogen-limited growth, repressed by TnrA.

224308:BSU 16300
gene id: 224308:BSU_16300
uniprot id: A0A6M3ZB41
uniprot id: P23452
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FliL family.
messages: Cell membrane
messages: Controls the rotational direction of flagella during chemotaxis.
messages: Membrane

224308:BSU 00780
gene id: 224308:BSU_00780
uniprot id: A0A6M4JCB7
uniprot id: P28823
names: Function
names: Similarity
messages: Belongs to the DHNA family.
messages: Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.

224308:BSU 32100
gene id: 224308:BSU_32100
uniprot id: O05267
names: Cofactor
names: Similarity
messages: Belongs to the NADH dehydrogenase family.
messages: Binds 1 FAD per subunit.

224308:BSU 15030
gene id: 224308:BSU_15030
uniprot id: O34765
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression is controlled by a sigma-E-regulated promoter which needs the sigma-E factor for the binding of the RNA polymerase and subsequent transcription.
messages: Required for spore cortex formation.

224308:BSU 17900
gene id: 224308:BSU_17900
uniprot id: P45707
names: Function
names: Induction
messages: Inhibits DNA replication in growing cells, thus reducing chromosome copy number and playing a significant role during the onset of sporulation.
messages: Up-regulated by spo0A.

224308:BSU 15000
gene id: 224308:BSU_15000
uniprot id: A0A6M3ZB05
uniprot id: O34658
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0298 family.
messages: Cytoplasm

224308:BSU 28820
gene id: 224308:BSU_28820
uniprot id: A0A6M4JN06
uniprot id: P94521
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the peptidase M42 family.
messages: Binds 2 divalent metal cations per subunit.
messages: Homododecamer.
messages: Putative aminopeptidase.

224308:BSU 33910
gene id: 224308:BSU_33910
uniprot id: A0A6M3ZGR4
uniprot id: P39773
names: Activity Regulation
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the BPG-independent phosphoglycerate mutase family.
messages: Binds 2 manganese ions per subunit.
messages: Essential for rapid growth and for sporulation. Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.
messages: Inhibited during sporulation by acidification of the forespore, thus allowing accumulation of the spore's large depot of 3-phosphoglyceric acid.
messages: Monomer.

224308:BSU 32450
gene id: 224308:BSU_32450
uniprot id: O32141
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced by TnrA during imiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or uric acid are added during limiting-nitrogen conditions.
messages: HIU and OHCU are unstable, they spontaneously decompose to form a racemic mixture of allantoin.
messages: In the C-terminal section; belongs to the uricase family.
messages: In the N-terminal section; belongs to the OHCU decarboxylase family.

224308:BSU 05870
gene id: 224308:BSU_05870
uniprot id: A0A6M3Z8F0
uniprot id: O05511
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the mannose-6-phosphate isomerase type 1 family.
messages: Binds 1 zinc ion per subunit.
messages: Seems to be involved in the degradation of glucomannan.
messages: Up-regulated by konjac glucomannan and by cellobiose and mannobiose, the possible degradation products of glucomannan. Repressed by glucose via the carbon catabolite repression system. Also repressed by GmuR.

224308:BSU 01500
gene id: 224308:BSU_01500
uniprot id: A0A6M3Z6X6
uniprot id: P21470
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uS9 family.
messages: Part of the 30S ribosomal subunit.

224308:BSU 15700
gene id: 224308:BSU_15700
uniprot id: A0A6M3ZFY5
uniprot id: O35033
names: Caution
names: Cofactor
names: Function
names: Similarity
messages: Binds 1 FMN per subunit.
messages: Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.
messages: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.
messages: In the C-terminal section; belongs to the PPC synthetase family.
messages: In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 36200
gene id: 224308:BSU_36200
uniprot id: P96721
names: Disruption Phenotype
messages: Deletion of the ywqH-ywqI-ywqJ-ywqK-nfi operon has no visible phenotype, however it is out-competed by wild-type cells.

224308:BSU 10490
gene id: 224308:BSU_10490
uniprot id: A0A6M4JF00
uniprot id: O07560
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.
messages: Belongs to the peptidase S26 family.
messages: Cell membrane
messages: Expressed constitutively.
messages: Membrane

224308:BSU 36020
gene id: 224308:BSU_36020
uniprot id: A0A6M4JPU1
uniprot id: Q04778
names: Function
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.
messages: Regulates the expression of the alsSD operon for acetoin biosynthesis.

224308:BSU 20770
gene id: 224308:BSU_20770
uniprot id: O34498
names: Subunit
messages: Homodimer.

224308:BSU 26930
gene id: 224308:BSU_26930
uniprot id: O07909
names: Similarity
messages: To B.subtilis YraJ.

224308:BSU 00450
gene id: 224308:BSU_00450
uniprot id: A0A6M4JET7
uniprot id: P37549
names: Function
names: Similarity
messages: Belongs to the alpha/beta-type SASP family.
messages: May play some important role in either sporulation or the dormant spore.

224308:BSU 38760
gene id: 224308:BSU_38760
uniprot id: A0A6M3ZHS0
uniprot id: P94364
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the cytochrome ubiquinol oxidase subunit 1 family.
messages: Binds 1 iron-chlorin (heme d or cytochrome d) per heterodimer, in conjunction with CydB.
messages: Binds 1 protoheme IX center (heme b558) per subunit.
messages: Binds 1 protoheme IX center (heme b595) per heterodimer, in conjunction with CydB.
messages: Cell membrane
messages: Heterodimer of subunits I and II.
messages: Membrane

224308:BSU 03860
gene id: 224308:BSU_03860
uniprot id: A0A6M4JCV2
uniprot id: P94424
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the flavin oxidoreductase frp family.
messages: Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism. The reduction of nitro-organic compounds and inorganic chromate occur in the absence of external FMN and are therefore believed to require binding of these substrates in the active site. In contrast to these reduction processes, azo dyes are reduced only in the presence of external FMN, indicating that azo dye reduction occurs outside the active site of the enzyme.
messages: FMN is a competitive inhibitor of NADH, and therefore leads to the preferential utilization of NADPH.
messages: Homodimer.
messages: Reduces FMNH(2) to FMN, with NADH or NADPH as reductant. It also reduces nitroaromatic compounds, quinones, chromates and azo dyes. It could supply the reduced form of FMN to luciferase-like protein and contribute to the degradation of aromatic compounds.
messages: Strongly induced by stress due to exposure to 6-brom-2-vinyl-chroman-4-on (chromanon) and less strongly induced after exposure to 2-methylhydroquinone (2-MHQ) or catechol stress.

224308:BSU 11180
gene id: 224308:BSU_11180
uniprot id: A0A6M4JRB8
uniprot id: P70952
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 14400
gene id: 224308:BSU_14400
uniprot id: A0A6M3ZAM2
uniprot id: P71012
names: Domain
names: Function
names: Subcellular Location Annotation
messages: Cell inner membrane
messages: Cell membrane
messages: Membrane
messages: The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.
messages: The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.
messages: The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.
messages: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.

224308:BSU 10870
gene id: 224308:BSU_10870
uniprot id: O06730
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LysE/ArgO transporter (TC 2.A.75) family.
messages: Cell membrane

224308:BSU 19440
gene id: 224308:BSU_19440
uniprot id: O31855
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.
messages: Cell membrane
messages: Multidrug efflux pump.

224308:BSU 39440
gene id: 224308:BSU_39440
uniprot id: P39139
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 26660
gene id: 224308:BSU_26660
uniprot id: A0A6M3ZEL4
uniprot id: P94502
names: Function
names: Similarity
messages: Belongs to the 4-oxalocrotonate tautomerase family.
messages: Putative target of GltR.

224308:BSU 08600
gene id: 224308:BSU_08600
uniprot id: Q45539
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyltransferase 2 family. GtrB subfamily.
messages: Cell membrane
messages: Induced by salt addition via sigma-B dependent promoter.

224308:BSU 16220
gene id: 224308:BSU_16220
uniprot id: A0A6M3ZG40
uniprot id: P23448
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliG family.
messages: Cell membrane
messages: Membrane
messages: One of the proteins that forms a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of flagellar rotation.

224308:BSU 21050
gene id: 224308:BSU_21050
uniprot id: A0A6M3ZG85
uniprot id: P68573
names: Function
names: Similarity
names: Subunit
messages: Belongs to the bacterial histone-like protein family.
messages: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
messages: Homodimer.

224308:BSU 30430
gene id: 224308:BSU_30430
uniprot id: O34953
names: Developmental Stage
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-5 integral membrane protein family.
messages: Cell membrane
messages: Expressed early in the stationary phase.
messages: Negatively regulated by YtrA.
messages: Part of the ABC transporter complex YtrBCDEF that plays a role in acetoin utilization during stationary phase and sporulation.
messages: The complex is composed of 2 ATP-binding proteins (YtrB and YtrE), 2 transmembrane proteins (YtrC and YtrD) and a solute-binding protein (YtrF).

224308:BSU 16310
gene id: 224308:BSU_16310
uniprot id: A0A6M3ZG51
uniprot id: P23453
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Bacterial flagellum basal body
messages: Belongs to the FliM family.
messages: Cell membrane
messages: Membrane
messages: One of the proteins that forms a switch complex that is proposed to be located at the base of the basal body. This complex interacts with chemotaxis proteins (such as CheY) in addition to contacting components of the motor that determine the direction of flagellar rotation.

224308:BSU 02800
gene id: 224308:BSU_02800
uniprot id: O34772
names: Similarity
messages: To B.subtilis YcdB.

224308:BSU 00990
gene id: 224308:BSU_00990
uniprot id: A0A6M3Z6S3
uniprot id: Q06798
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
messages: An unknown mutation in this protein gives rise to thiostrepton resistance.
messages: Belongs to the bacterial ribosomal protein bL33 family.
messages: No effect on sporulation at 37 degrees Celsius, however sporulation decreases at 47 degrees Celsius.
messages: Plays a role in sporulation at high temperatures.

224308:BSU 01810
gene id: 224308:BSU_01810
uniprot id: P19219
names: Cofactor
names: Function
names: Induction
messages: Binds 1 zinc ion per subunit.
messages: Is involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs the methylphosphotriester lesions in DNA by a direct and irreversible transfer of the methyl group to one of its own cysteine residues.
messages: The methylation of AdaA by methylphosphotriesters in DNA leads to its activation as a transcriptional regulator that activates the transcription of the ada operon which consists of adaA and adaB, and of the adjacent gene alkA.
messages: Up-regulated by methylated AdaA itself in response to the exposure to alkylating agents such as MNNG.

224308:BSU 39340
gene id: 224308:BSU_39340
uniprot id: A0A6M3ZHN1
uniprot id: P10943
names: Function
names: Similarity
names: Subunit
messages: Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization.
messages: Belongs to the HutP family.
messages: Homohexamer.

224308:BSU 28870
gene id: 224308:BSU_28870
uniprot id: A0A6M3ZJ46
uniprot id: P55872
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the IF-3 family.
messages: Cytoplasm
messages: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.
messages: Monomer.

224308:BSU 34030
gene id: 224308:BSU_34030
uniprot id: A0A6M3ZH36
uniprot id: O32259
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the LutC/YkgG family.
messages: Cells lacking this gene are unable to grow on minimal medium with L-lactate as the sole carbon source. Cells lacking the lutABC operon exhibit little or no defect in biofilm formation on MSgg medium, but form small colonies that almost completely lacked surface architecture when glycerol is replaced with L-lactate in the MSgg medium.
messages: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. May also allow cells to utilize an alternative carbon source during biofilm formation, since it contributes to the formation of architecturally complex communities when lactate is present.
messages: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source.
messages: Is under the dual control of the transcriptional repressors LutR and SinR, which allows the lutABC operon to be induced during both growth in liquid culture and biofilm formation. Is induced by L-lactate, which relieves LutR repression.

224308:BSU 24600
gene id: 224308:BSU_24600
uniprot id: P23308
names: Function
names: Subunit
messages: Acts as an antagonist to SinR. SinI prevents SinR from binding to its target sequence on the gene for AprE.
messages: Heterodimer with SinR.

224308:BSU 09410
gene id: 224308:BSU_09410
uniprot id: P19406
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the alkaline phosphatase family.
messages: Binds 1 Mg(2+) ion.
messages: Binds 2 Zn(2+) ions.
messages: Monomer.

224308:BSU 07920
gene id: 224308:BSU_07920
uniprot id: A0A6M3Z8I9
uniprot id: O34840
names: Activity Regulation
names: Caution
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.
messages: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. Cation/proton exchanger (CAX) subfamily.
messages: Ca(+)/H(+) antiporter that extrudes calcium in exchange for external protons.
messages: Ca(+)/H(+) antiporter that extrudes calcium in exchange for external protons. Does not transport sodium or potassium.
messages: Calcium efflux is tightly regulated by intracellular pH.
messages: Cell membrane
messages: Homotrimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane
messages: Transcriptionally regulated by the forespore-specific sigma factor, SigG, and the general stress response regulator, SigB.

224308:BSU 26000
gene id: 224308:BSU_26000
uniprot id: P45933
names: Similarity
messages: To B.subtilis XkdR.

224308:BSU 09620
gene id: 224308:BSU_09620
uniprot id: O07592
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the glycerophosphoryl diester phosphodiesterase family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Glycerophosphodiester phosphodiesterase hydrolyzes glycerophosphodiesters into glycerol-3-phosphate (G3P) and the corresponding alcohol.

224308:BSU 13060
gene id: 224308:BSU_13060
uniprot id: A0A6M4JKY7
uniprot id: P49853
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0702 family.
messages: Cell membrane
messages: Membrane

224308:BSU 33530
gene id: 224308:BSU_33530
uniprot id: O32223
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the Gfo/Idh/MocA family.
messages: Catalyzes the reversible NADPH-dependent reduction of scyllo-inosose (SIS) to scyllo-inositol (SI). Cannot use NADH instead of NADPH. May be involved in reduction of not only SIS but also various oxidized compounds manifested upon stressful conditions.
messages: Cells lacking this gene show normal growth on SI as well as on glucose or myo-inositol (MI) as the sole carbon source.
messages: Is constitutively expressed, even in the absence of both myo-inositol and scillo-inositol (PubMed:20133360). Is up-regulated under certain stressful conditions (PubMed:22383849).

224308:BSU 36540
gene id: 224308:BSU_36540
uniprot id: P71045
names: Developmental Stage
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expressed during sporulation.
messages: Expression is sigma F-dependent.
messages: May be important for peptidoglycan remodeling.

224308:BSU 16000
gene id: 224308:BSU_16000
uniprot id: A0A6M4JGN3
uniprot id: O31738
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A probable RNA chaperone. Forms a complex with KhpB which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.
messages: Belongs to the KhpA RNA-binding protein family.
messages: Cytoplasm
messages: Forms a complex with KhpB.

224308:BSU 29330
gene id: 224308:BSU_29330
uniprot id: A0A6M3ZFM1
uniprot id: O34846
names: Function
names: Similarity
messages: Involved in a cysteine salvage pathway from S-alkylcysteine. Catalyzes the oxidation of N-acetyl-S-benzyl-L-cysteine and N-acetyl-S-methyl-L-cysteine to (R)-N-acetyl-S-benzyl-L-cysteine sulfoxide and (R)-N-acetyl-S-methyl-L-cysteine sulfoxide, respectively. This pathway is likely important in the catabolism of alkylated cysteine generated by proteolysis of alkylated glutathione formed in the detoxification of a wide range of electrophiles.
messages: To bacterial alkanal monooxygenase alpha and beta chains.

224308:BSU 32310
gene id: 224308:BSU_32310
uniprot id: O32127
names: Function
names: Subunit
messages: Homodimer, probably forms a complex with cognate toxin YutE.
messages: Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Probably neutralizes cognate toxin YutE.

224308:BSU 12790
gene id: 224308:BSU_12790
uniprot id: P39798
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Associated with cell lysis upon induction of PbsX.
messages: Cell membrane
messages: To B.licheniformis xpaF1 and xpaL1.

224308:BSU 07670
gene id: 224308:BSU_07670
uniprot id: O34905
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 28260
gene id: 224308:BSU_28260
uniprot id: A0A6M4JKQ5
uniprot id: P80858
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: By superoxide.
messages: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.
messages: Heterodimer of LeuC and LeuD.

224308:BSU 10590
gene id: 224308:BSU_10590
uniprot id: O07570
names: Similarity
messages: In the C-terminal section; belongs to the bacterial solute-binding protein 5 family.

224308:BSU 28660
gene id: 224308:BSU_28660
uniprot id: A0A6M4JKV2
uniprot id: P94537
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspI family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Disappears after 45 minutes of spore germination. Expression is sigma G-dependent.
messages: Spore core

224308:BSU 30880
gene id: 224308:BSU_30880
uniprot id: A0A6M3ZFM2
uniprot id: O34413
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

224308:BSU 29240
gene id: 224308:BSU_29240
uniprot id: O34460
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: Expression requires the sigma-E factor SigE.
messages: Involved in sporulation.

224308:BSU 38900
gene id: 224308:BSU_38900
uniprot id: P55183
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Probable member of the two-component regulatory system YxjM/YxjL. May activate YxjL by phosphorylation.

224308:BSU 34590
gene id: 224308:BSU_34590
uniprot id: A0A6M3ZH94
uniprot id: O06991
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex involved in maltodextrin import. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (MsmX), two transmembrane proteins (MdxF and MdxG) and a solute-binding protein (MdxE).

224308:BSU 23850
gene id: 224308:BSU_23850
uniprot id: A0A6M4JL49
uniprot id: P54547
names: Function
names: Similarity
messages: Belongs to the glucose-6-phosphate dehydrogenase family.
messages: Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.

224308:BSU 40620
gene id: 224308:BSU_40620
uniprot id: P37494
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 24480
gene id: 224308:BSU_24480
uniprot id: P54516
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 21720
gene id: 224308:BSU_21720
uniprot id: P54180
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 33470
gene id: 224308:BSU_33470
uniprot id: A0A6M4JP64
uniprot id: O32217
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: A late competence gene, expression is enhanced in the presence of ComK.
messages: Belongs to the DsbB family. BdbC subfamily.
messages: Cell membrane
messages: Cells partially restore cytochrome c oxidase activity in a CcdA-deficient mutant, possibly because the bacteria can no longer oxidize the two heme-binding thiol groups in apocytochrome c.
messages: Membrane
messages: Membrane raft
messages: Required for disulfide bond formation in some proteins.
messages: Required for the stabilization, possibly via formation of a disulfide bond, of the obligatory competence protein ComGC. Not normally required for production of the secreted lantibiotic sublancin 168, although it can partially substitute for BdbB when the latter is absent. It may also be required for the stability of other secreted proteins. Not required for sporulation.

224308:BSU 28099
gene id: 224308:BSU_28099
uniprot id: C0H465
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 03990
gene id: 224308:BSU_03990
uniprot id: A0A6M3Z7G7
uniprot id: P42957
names: Similarity
messages: Belongs to the mannitol dehydrogenase family.

224308:BSU 06520
gene id: 224308:BSU_06520
uniprot id: A0A6M4JKT5
uniprot id: P12048
names: Domain
names: Similarity
messages: Belongs to the PurH family.
messages: The IMP cyclohydrolase activity resides in the N-terminal region.

224308:BSU 29890
gene id: 224308:BSU_29890
uniprot id: O34760
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: By the antimicrobial streptomycin, produced by the Gram-positive bacterium S.griseus.
messages: Probable hydrolase that is able to inhibit the signaling pathway required for the streptomycin production and development of aerial mycelium in S.griseus. Thus, serves as a defensive strategy against competing bacteria. The putative target for YtnP may be a gamma-butyrolactone termed A factor, which is the quorum-sensing signaling molecule that positively regulates streptomycin production and development of aerial hyphae in S.griseus.

224308:BSU 05660
gene id: 224308:BSU_05660
uniprot id: P96707
names: Similarity
messages: Belongs to the nitroreductase family.

224308:BSU 05110
gene id: 224308:BSU_05110
uniprot id: P96658
names: Similarity
messages: Belongs to the peptidase C56 family.

224308:BSU 22370
gene id: 224308:BSU_22370
uniprot id: A0A6M3ZD26
uniprot id: P53001
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
messages: Cytoplasm
messages: Homodimer.

224308:BSU 00940
gene id: 224308:BSU_00940
uniprot id: A0A6M4JEP9
uniprot id: Q06752
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family.
messages: Binds 1 zinc ion per subunit.
messages: Cytoplasm
messages: Monomer.

224308:BSU 25300
gene id: 224308:BSU_25300
uniprot id: A0A6M4JLI6
uniprot id: P19079
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the cytidine and deoxycytidylate deaminase family.
messages: Binds 1 zinc ion per subunit.
messages: Homotetramer.
messages: This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.

224308:BSU 06640
gene id: 224308:BSU_06640
uniprot id: O34640
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the pseudomonas-type ThrB family.
messages: Knockout mutant is susceptible to amicoumacin A.
messages: Phosphorylates the antibiotic amicoumacin A (Ami), leading to its inactivation. Mediates natural B.subtilis resistance to the drug.

224308:BSU 26950
gene id: 224308:BSU_26950
uniprot id: A0A6M3ZES1
uniprot id: O07919
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Spore coat

224308:BSU 33630
gene id: 224308:BSU_33630
uniprot id: A0A6M4JP81
uniprot id: O32233
names: Caution
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SecG family.
messages: Cell membrane
messages: Constitutively expressed, part of a 5 gene operon with multiple promoters. Not ethanol-stress induced.
messages: Involved in protein export. Participates in an early event of protein translocation (By similarity).
messages: Involved in protein export. Participates in an early event of protein translocation.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Membrane

224308:BSU 24780
gene id: 224308:BSU_24780
uniprot id: P54502
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 14610
gene id: 224308:BSU_14610
uniprot id: A0A6M4JLF8
uniprot id: P21880
names: Cofactor
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
messages: Binds 1 FAD per subunit.
messages: Catalyzes the oxidation of dihydrolipoamide to lipoamide.
messages: Cytoplasm
messages: Homodimer. Component of two multienzyme complexes: pyruvate dehydrogenase complex and oxoglutarate dehydrogenase complex.
messages: The active site is a redox-active disulfide bond.

224308:BSU 23910
gene id: 224308:BSU_23910
uniprot id: A0A6M3ZIV7
uniprot id: P54542
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the peptidase M20A family.
messages: Belongs to the peptidase M42 family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Binds 2 divalent metal cations per subunit.
messages: Could be a peptidase.

224308:BSU 04600
gene id: 224308:BSU_04600
uniprot id: P96616
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0699 family.
messages: Cell membrane

224308:BSU 34730
gene id: 224308:BSU_34730
uniprot id: A0A6M3ZGB0
uniprot id: O06977
names: Similarity
messages: Belongs to the arylamine N-acetyltransferase family.

224308:BSU 15130
gene id: 224308:BSU_15130
uniprot id: A0A6M3ZIX6
uniprot id: P55343
names: Disruption Phenotype
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the MraZ family.
messages: Forms oligomers.
messages: No visible phenotype.
messages: Transcribed at a constant level in all growth phases. Part of the mraZ-rsmH-ftsL-pbpB operon.
messages: nucleoid

224308:BSU 17890
gene id: 224308:BSU_17890
uniprot id: A0A6M4JIM5
uniprot id: P45694
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the transketolase family.
messages: Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.
messages: Homodimer.

224308:BSU 28500
gene id: 224308:BSU_28500
uniprot id: A0A6M3ZI45
uniprot id: P14949
names: Function
names: Similarity
messages: Belongs to the thioredoxin family.
messages: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.

224308:BSU 03930
gene id: 224308:BSU_03930
uniprot id: P12310
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Homotetramer.
messages: Induced at stage III of sporulation.

224308:BSU 02920
gene id: 224308:BSU_02920
uniprot id: O34447
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TerC family.
messages: Cell membrane

224308:BSU 19720
gene id: 224308:BSU_19720
uniprot id: A0A6M3ZCL0
uniprot id: O34466
names: Similarity
names: Subunit
messages: Belongs to the 3-oxoacid CoA-transferase subunit B family.
messages: Heterodimer of a subunit alpha and a subunit beta.

224308:BSU 15520
gene id: 224308:BSU_15520
uniprot id: A0A6M4JG47
uniprot id: P25994
names: Caution
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the CarB family.
messages: Binds 4 Mg(2+) or Mn(2+) ions per subunit.
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 33650
gene id: 224308:BSU_33650
uniprot id: O32235
names: Subcellular Location Annotation
messages: Cytoplasm

224308:BSU 27230
gene id: 224308:BSU_27230
uniprot id: O05396
names: Similarity
messages: To A.fulgidus AF1717.

224308:BSU 23600
gene id: 224308:BSU_23600
uniprot id: P38423
names: Similarity
messages: To M.jannaschii MJ0043 N-terminal region.

224308:BSU 08180
gene id: 224308:BSU_08180
uniprot id: A0A6M4JJN1
uniprot id: P54716
names: Activity Regulation
names: Cofactor
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Because it hydrolyzes 6-phospho-alpha-glucopyranosides without activated leaving groups (such as maltose-6'-phosphate) but not glycosidic linkage of naturally occurring phospho-beta-glucosides such as cellobiose-6'-phosphate nor methyl 6-phospho-beta-D-glucopyranoside, GlvA is certainly more appropriately classified as a 6-phospho-alpha-glucosidase than as a 6-phospho-beta-glucosidase. The ability to hydrolyze beta-glycosidic linkages is exceptional and applies only to activated 6-phospho-beta-D-glucopyranosides.
messages: Belongs to the glycosyl hydrolase 4 family.
messages: Binds 1 NAD(+) per subunit.
messages: Binds 1 NAD(+) per subunit. Is only active with NAD(+), not NADH.
messages: Binds 1 divalent metal cation per subunit. Manganese, iron, cobalt or nickel enhance activity.
messages: By maltose; repressed by glucose.
messages: Cellobiose-6'-phosphate and 6-phospho-beta-D-glucopyranoside are not substrates but competitive inhibitors of GlvA.
messages: Homotetramer.
messages: Hydrolyzes maltose-6'-phosphate and trehalose-6'-phosphate. Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system (PEP-PTS). Is also able to significantly catalyze the hydrolysis of both 6-phospho-alpha- and 6-phospho-beta-glucosides containing activated leaving groups such as p-nitrophenol and does so with retention and inversion, respectively, of the substrate anomeric configuration.
messages: Reaction proceeds via a redox-elimination-addition mechanism consistent with an E1cb-type mechanism. This includes redox steps involving NAD(+) and stabilization of intermediates by Mn(2+).

224308:BSU 18150
gene id: 224308:BSU_18150
uniprot id: A0A6M4JGU4
uniprot id: Q45070
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 30 family.
messages: Catalyzes the depolymerization of methylglucuronoxylan (MeGAXn) from different sources. It cleaves the beta-1,4-xylosidic bond penultimate to that linking carbon one of the xylose residue substituted with alpha-1,2-linked 4-O-methyl-D-glucuronate (MeGA).
messages: Constitutively expressed.
messages: Secreted

224308:BSU 16430
gene id: 224308:BSU_16430
uniprot id: P29072
names: Function
names: Subcellular Location Annotation
messages: Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to CheB, CheY or CheV.
messages: Membrane

224308:BSU 18000
gene id: 224308:BSU_18000
uniprot id: A0A6M3ZBI8
uniprot id: P09339
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the aconitase/IPM isomerase family.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: By citrate via CcpC. When citrate is absent, CcpC binds to the sites near the citB promoter and blocks expression. When citrate is present, it causes a change in the interaction of CcpC with its binding sites, resulting in derepression of citB. When citrate is very abundant, CcpC activates citB expression, presumably reflecting a change in the interaction of CcpC with RNA polymerase. Also induced by decoyinine and nutrient depletion.
messages: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
messages: Cells lacking this gene show a dramatic increase in the concentration of citrate. This accumulation of citrate prevents sporulation due to chelation by citrate of divalent cations required for proper functioning of the Spo0A-initiated phosphorelay.
messages: Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate (PubMed:3110133, PubMed:23354745). Also catalyzes the rehydration of 2-methyl-cis-aconitate to produce 2-methylisocitrate (PubMed:28956599). The apo form of AcnA functions as a RNA-binding regulatory protein which plays a role in the regulation of citrate concentration and in the sporulation. To prevent the accumulation of excessive levels of citrate, it binds near the 5' end of the citZ mRNA, decreasing its stability and thereby limiting the concentration of citrate synthase in the cell. Aconitase also binds to the gerE transcript late in sporulation and stabilizes it for translation, thereby increasing the rate and level of GerE protein accumulation (PubMed:10468622, PubMed:16923907, PubMed:23354745, PubMed:9393699).
messages: Monomer.
messages: Mutagenesis of different positions (F662L, R741F, Q745E, I810T and V853A) on the C-terminal region of citB gene product shows that even B.subtilis mutant has high aconitase catalytic activity, it is defective in sporulation. The defect is at a late stage of sporulation, specifically affecting expression of sigma-K-dependent genes, many of which are important for spore coat assembly and require transcriptional activation by GerE. It strongly suggests that aconitase RNA binding activity may stabilize gerE mRNA in order to allow efficient GerE synthesis and proper timing of spore coat assembly.
messages: Whether aconitase is active as an enzyme or an RNA-binding protein is determined by the status of an iron-sulfur (4Fe-4S) cluster that is essential for the catalytic activity of all aconitases. Citrate is both cotransported with iron and a chelator of divalent cations, including iron. If the intracellular citrate level becomes excessive, iron will be sequestered away from iron-containing proteins, including aconitase. Since excess citrate greatly stimulates aconitase synthesis via the positive regulatory effect of CcpC, the cell will gain the ability to metabolize citrate at a higher rate. If so much iron has been sequestered that aconitase loses enzymatic activity, the cell will acquire a high concentration of enzymatically inactive but RNA-binding-competent aconitase molecules. These aconitase proteins can bind to the citZ mRNA and reduce the rate of citrate accumulation by restricting the synthesis of citrate synthase protein.

224308:BSU 16970
gene id: 224308:BSU_16970
uniprot id: O31775
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the YmdB-like family.
messages: Corresponds to iron 1 in the structure.
messages: Corresponds to iron 2 in the structure.
messages: Cytoplasm
messages: Deletion of the gene affects the levels of over 800 mRNAs (PubMed:24163345). Deletion mutant overexpresses flagellin and other genes of the sigma-D-dependent motility regulon. In contrast, the two major operons for biofilm formation are not expressed and the mutant is unable to form biofilms. All cells are short and motile (PubMed:21856853). Deletion also results in a significant decrease in intercellular molecular exchange (PubMed:26906740). Mutant displays aberrant developmental patterns and forms smaller colonies (PubMed:26904951).
messages: Homodimer.
messages: Plays a central, regulatory role in the late adaptive responses and affects the levels of many genes (PubMed:24163345). May act via regulation of cAMP levels (PubMed:26904951). Decreases the expression of motility genes and induces genes involved in biofilm formation, by controlling the expression of SlrR (PubMed:21856853). Required for formation of intercellular nanotubes that bridge neighboring cells to allow molecular exchange (PubMed:26906740). Plays a key role in directing the early stages of colony development (PubMed:26904951). In vitro, has a metal-dependent phosphodiesterase activity against 2',3'-cAMP and 2',3'-cGMP. Has also 3',5'-cyclic-nucleotide phosphodiesterase activity, but cannot use cyclic di-AMP or cyclic di-GMP, and does not have phosphatase activity (PubMed:24163345).

224308:BSU 10740
gene id: 224308:BSU_10740
uniprot id: O06723
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotH family.
messages: Involved in the assembly of several proteins in the inner and outer layer of the spore coat.
messages: Spore coat

224308:BSU 05050
gene id: 224308:BSU_05050
uniprot id: P96652
names: Function
messages: Negative regulation of glyA transcription and kinB-dependent sporulation.

224308:BSU 04780
gene id: 224308:BSU_04780
uniprot id: O31489
names: Caution
messages: Was originally (Ref.1) thought to be two genes, ydcI and ydcJ.

224308:BSU 36300
gene id: 224308:BSU_36300
uniprot id: P94591
names: Function
messages: Plays a role in carbon catabolite repression (CCR). Specifically required for transcriptional repression of the levanase operon by glucose but not by other sugars.

224308:BSU 19560
gene id: 224308:BSU_19560
uniprot id: O34842
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.
messages: Cytoplasm
messages: Putative hydrolase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 05650
gene id: 224308:BSU_05650
uniprot id: A0A6M4JDH9
uniprot id: P96706
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 26630
gene id: 224308:BSU_26630
uniprot id: A0A6M3ZJE4
uniprot id: P71025
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 07040
gene id: 224308:BSU_07040
uniprot id: O31525
names: Induction
messages: Up-regulated by growth on type I rhamnogalacturonan.

224308:BSU 32850
gene id: 224308:BSU_32850
uniprot id: A0A6M4JND9
uniprot id: O32179
names: Cofactor
names: Function
names: Induction
names: Similarity
messages: Belongs to the proline dehydrogenase family.
messages: Binds 1 FAD per subunit.
messages: Converts proline to delta-1-pyrroline-5-carboxylate.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize fadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 38920
gene id: 224308:BSU_38920
uniprot id: A0A6M4JQL8
uniprot id: P55179
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M20B family.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Cleaves the N-terminal amino acid of tripeptides.
messages: Cytoplasm

224308:BSU 32490
gene id: 224308:BSU_32490
uniprot id: O32145
names: Function
names: Induction
names: Subunit
messages: Could be composed of four subunits: PucA, PucC, PucD and PucE.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
messages: Oxidizes hypoxanthine and xanthine to uric acid.

224308:BSU 21430
gene id: 224308:BSU_21430
uniprot id: O31984
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SPP1 holin family.
messages: Cell membrane
messages: May be involved in the secretion of the autolysin BlyA.

224308:BSU 01570
gene id: 224308:BSU_01570
uniprot id: P50865
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide deacetylase family.
messages: Forespore
messages: Necessary to maintain spores after the late stage of sporulation. Might be involved in cortex formation.
messages: Negatively regulated by SpoIIID.

224308:BSU 34070
gene id: 224308:BSU_34070
uniprot id: Q795K2
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Member of the two-component regulatory system YvfT/YvfU. Probably activates YvfU by phosphorylation.

224308:BSU 01270
gene id: 224308:BSU_01270
uniprot id: A0A6M4JF23
uniprot id: P0CI78
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the universal ribosomal protein uL24 family.
messages: Cytoplasm
messages: Has also been isolated as a basic, heat-shock stable DNA-binding protein from the B.subtilis nucleoid. It binds cooperatively to double-stranded supercoiled DNA which it further compacts into complexes 15-17 nm in diameter. Overexpression of the protein disrupts nucleoid segregation and positioning.
messages: One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.
messages: One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.
messages: Part of the 50S ribosomal subunit.
messages: nucleoid

224308:BSU 01460
gene id: 224308:BSU_01460
uniprot id: P70970
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.
messages: Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.
messages: Cell membrane
messages: Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).

224308:BSU 30490
gene id: 224308:BSU_30490
uniprot id: O34614
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the methyltransferase superfamily. MnmM family.
messages: Homodimer.
messages: Involved in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA (PubMed:36762482). Catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34 to form mnm(5)s(2)U34 (PubMed:36762482).
messages: Mutant accumulates nm(5)s(2)U34 and cannot form mnm(5)s(2)U34.

224308:BSU 19220
gene id: 224308:BSU_19220
uniprot id: A0A6M3ZBW7
uniprot id: O34748
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the helicase family. RecQ subfamily.
messages: Cells lacking this gene are moderately sensitive to DNA-damaging agents. Sensitivity increases in the presence of mutated AddAB nuclease.
messages: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with RecS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks.
messages: nucleoid

224308:BSU 30080
gene id: 224308:BSU_30080
uniprot id: P94496
names: Induction
messages: Transcription starts around hour 2 of sporulation and requires sigma-E.

224308:BSU 29780
gene id: 224308:BSU_29780
uniprot id: P40779
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0478 family.
messages: Membrane

224308:BSU 11420
gene id: 224308:BSU_11420
uniprot id: A0A6M3Z9S8
uniprot id: P21656
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family.
messages: Catalyzes the attachment of tryptophan to tRNA(Trp).
messages: Cytoplasm
messages: Homodimer.

224308:BSU 39930
gene id: 224308:BSU_39930
uniprot id: A0A6M4JQW5
uniprot id: P42112
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Drug:H(+) antiporter-3 (DHA3) (TC 2.A.1.21) family.
messages: Cell membrane
messages: Membrane

224308:BSU 26440
gene id: 224308:BSU_26440
uniprot id: P54442
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in transport.

224308:BSU 13900
gene id: 224308:BSU_13900
uniprot id: A0A6M4JG04
uniprot id: P08877
names: Activity Regulation
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the HPr family.
messages: Cytoplasm
messages: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.
messages: P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity.
messages: Phosphorylation on Ser-46 inhibits the phosphoryl transfer from enzyme I to HPr.

224308:BSU 37830
gene id: 224308:BSU_37830
uniprot id: A0A6M4JQG9
uniprot id: P39630
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.
messages: Binds 1 NAD(+) per subunit.
messages: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction.
messages: Homodimer.

224308:BSU 20880
gene id: 224308:BSU_20880
uniprot id: O31929
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02680
gene id: 224308:BSU_02680
uniprot id: O34619
names: Function
messages: Acts as repressor of the lincomycin-resistance (lmrAB) and yxaGH operons.

224308:BSU 04720
gene id: 224308:BSU_04720
uniprot id: A0A6M4JFQ5
uniprot id: P17904
names: Activity Regulation
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the anti-sigma-factor family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Homodimer. In stressed cells, forms a complex with RsbV. The predominant form of this complex has a stoichiometry of 2:2 (one dimer of RsbW is bound by two monomers of RsbV). In unstressed cells, forms a 2:1 complex with sigma-B.
messages: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, RsbV. Upon phosphorylation of RsbV, RsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B).
messages: The higher affinity of RsbW for RsbV than for sigma-B, rather than a difference in the concentrations of RsbV and sigma-B, is the driving force that is responsible for the switch of RsbW to non-phosphorylated RsbV. The kinase activity of RsbW is directly regulated by changes in the ATP/ADP ratio.

224308:BSU 10980
gene id: 224308:BSU_10980
uniprot id: A0A6M4JH79
uniprot id: Q796Q1
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 08610
gene id: 224308:BSU_08610
uniprot id: O31582
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 36660
gene id: 224308:BSU_36660
uniprot id: A0A6M3ZH40
uniprot id: P75030
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the urease gamma subunit family.
messages: Cytoplasm
messages: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.

224308:BSU 01900
gene id: 224308:BSU_01900
uniprot id: O31421
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 37620
gene id: 224308:BSU_37620
uniprot id: P39650
names: Developmental Stage
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Expressed during sporulation.
messages: Expression is sigma F and sigma G-dependent.
messages: Forespore
messages: Seems to improve the efficiency of sporulation by fine-tuning the expression of genes in the sigma F regulon, particularly the timing of their expression. Negatively regulates spoIIR and its own synthesis.

224308:BSU 04340
gene id: 224308:BSU_04340
uniprot id: A0A6M4JFA5
uniprot id: P96591
names: Cofactor
names: Induction
names: Similarity
messages: Belongs to the TPP enzyme family.
messages: Binds 1 Mg(2+) ion per subunit.
messages: Binds 1 thiamine pyrophosphate per subunit.
messages: Transcriptionally regulated by SigB.

224308:BSU 34120
gene id: 224308:BSU_34120
uniprot id: A0A6M3ZGT1
uniprot id: O07013
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 53 family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Involved in galactan degradation (PubMed:17056685, PubMed:27501980). Degrades arabinose-free galactan to galactooligosaccharides, producing galactotetraose as the main product along with galactotriose, galactobiose, and galactose (PubMed:27501980). Is also able to degrade galactotetraose, galactotriose and galactobiose, suggesting an additional exo-mode of activity (PubMed:27501980). May hydrolyze the beta-1,4-galactan linkages of the galactan portion of arabinogalactan type I, a pectic plant polysaccharide from which most of the arabinose has been removed (Probable).
messages: Repressed by the transcriptional regulator GanR and induced by galactobiose. Also repressed by glucose.
messages: Secreted

224308:BSU 01100
gene id: 224308:BSU_01100
uniprot id: A0A6M3Z6L4
uniprot id: P21472
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: A number of substitutions and deletions can promote streptomycin resistance, dependence or pseudodependence; all but one of these (K55R) are associated with hyperaccurate translation.
messages: Belongs to the universal ribosomal protein uS12 family.
messages: Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).
messages: Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.
messages: Many streptomycin resistant B.subtilis with mutations in this protein produce increased quantities of a natural (but uncharacterized) antibiotic.
messages: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.
messages: The enzyme that would modify Asp-102 to 3-methylthioaspartic acid has not been found in the proteome of this organism, and that modification does not occur.
messages: With S4 and S5 plays an important role in translational accuracy.

224308:BSU 34240
gene id: 224308:BSU_34240
uniprot id: P71063
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the transferase hexapeptide repeat family.
messages: Catalyzes the conversion of UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose to UDP-2,4,6-trideoxy-2,4-diacetamido glucose, commonly known as UDP-N,N'-diacetylbacillosamine (UDP-diNAcBac).
messages: Forms oligomers.
messages: Insertion mutant shows decreased efficiency of biofilm formation. Disruption affects swarming motility.
messages: Repressed by SinR.

224308:BSU 24550
gene id: 224308:BSU_24550
uniprot id: A0A6M4JLQ9
uniprot id: P54377
names: Function
names: Similarity
names: Subunit
messages: Belongs to the GcvP family. C-terminal subunit subfamily.
messages: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (By similarity).
messages: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.
messages: The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.

224308:BSU 04370
gene id: 224308:BSU_04370
uniprot id: A0A6M4JK71
uniprot id: P96594
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0410 family.
messages: Cell membrane
messages: Membrane

224308:BSU 30980
gene id: 224308:BSU_30980
uniprot id: A0A6M3ZFN0
uniprot id: P39118
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.
messages: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.
messages: Expressed exclusively on media containing carbon sources that allow efficient sporulation.
messages: Monomer.

224308:BSU 17700
gene id: 224308:BSU_17700
uniprot id: P07790
names: Similarity
messages: To B.subtilis protein YnzH.

224308:BSU 05720
gene id: 224308:BSU_05720
uniprot id: O05496
names: Similarity
messages: Belongs to the UDP-glycosyltransferase family.

224308:BSU 11160
gene id: 224308:BSU_11160
uniprot id: P70949
names: Function
names: Similarity
messages: Belongs to the MIP18 family.
messages: Involved in the maturation of iron-sulfur (Fe-S) proteins. May function as a Fe-S cluster carrier.

224308:BSU 31150
gene id: 224308:BSU_31150
uniprot id: A0A6M4JMX6
uniprot id: P94507
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.
messages: Belongs to the UppP family.
messages: Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.
messages: Cell membrane
messages: Membrane

224308:BSU 14880
gene id: 224308:BSU_14880
uniprot id: A0A6M3ZAR5
uniprot id: P24009
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
messages: Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.
messages: Cell membrane
messages: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.
messages: Interacts with CtaA.
messages: Membrane

224308:BSU 23780
gene id: 224308:BSU_23780
uniprot id: A0A6M3ZDR3
uniprot id: P54554
names: Similarity
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

224308:BSU 30700
gene id: 224308:BSU_30700
uniprot id: A0A6M4JNS9
uniprot id: O34967
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL31 family. Type B subfamily.
messages: Only found in ribosomes after the end of exponential growth. In PubMed:12904577 its expression was predicted to be repressed by the zinc-specific metallo-regulatory protein zur; in PubMed:15049826 it was shown that its expression is indeed repressed by zur.
messages: Part of the 50S ribosomal subunit after the end of exponential growth.
messages: Part of the 50S ribosomal subunit.
messages: While neither of the L31 paralogs is essential, this protein does not seem to function as the main L31 protein. Has a higher affinity for 70S ribosomes than the zinc-containing L31 paralog; is able to displace it to varying extents, even under zinc-replete conditions.

224308:BSU 03140
gene id: 224308:BSU_03140
uniprot id: P12921
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Involved in the resistance to tunicamycin. Binds ATP.

224308:BSU 05970
gene id: 224308:BSU_05970
uniprot id: A0A6M3Z8L9
uniprot id: O05521
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the transcriptional regulatory Rex family.
messages: Cytoplasm
messages: Homodimer.
messages: Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.

224308:BSU 40330
gene id: 224308:BSU_40330
uniprot id: A0A6M4JR60
uniprot id: P39137
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family.
messages: Cell membrane
messages: Membrane
messages: Part of the rocDEF operon. Expression is sigma L dependent, induced by arginine, ornithine or proline.
messages: Putative transport protein involved in arginine degradative pathway. Probably transports arginine or ornithine.

224308:BSU 18360
gene id: 224308:BSU_18360
uniprot id: P39840
names: Developmental Stage
names: Induction
names: Similarity
messages: Belongs to the aldose epimerase family.
messages: By nitrogen or tryptophan starvation.
messages: Expressed at the end of the exponential growth phase.

224308:BSU 06030
gene id: 224308:BSU_06030
uniprot id: A0A6M3Z7Y6
uniprot id: P28598
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the chaperonin (HSP60) family.
messages: Cytoplasm
messages: Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.

224308:BSU 02950
gene id: 224308:BSU_02950
uniprot id: A0A6M3Z766
uniprot id: O34691
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.
messages: Cell membrane
messages: Membrane
messages: Partially reduces sensitivity to the toxic niacin analog 6-amino-nicotinamide.
messages: Probably involved in the uptake of amidated and deamidated forms of niacin. Increases the growth rate of E.coli that is unable to make niacin de novo; confers increased sensitivity to the toxic niacin analog 6-amino-nicotinamide to wild-type E.coli. There is probably another mechanism for niacin uptake.

224308:BSU 27590
gene id: 224308:BSU_27590
uniprot id: A0A6M4JMB1
uniprot id: O32042
names: Biotechnology
names: Caution
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.
messages: A non-functional D-aminoacyl-tRNA deacylase (PubMed:4292198, PubMed:24097941, PubMed:25441601).
messages: An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
messages: Belongs to the DTD family.
messages: Could be the product of a pseudogene. The lack of an initiation codon prevents translation of this gene, in agreement with the observation that D-Tyr-tRNA(Tyr) deacylase activity cannot be detected in some strains of B.subtilis (PubMed:4292198, PubMed:24097941, PubMed:25441601). Mutagenesis to restore the start codon (in strain NCIB 3610, the parent of the fully sequenced 168) confers resistance to D-Tyr (PubMed:24097941).
messages: Culture in the presence of D-Leu, D-Met, D-Trp or D-Tyr leads to growth inhibition due to their incorporation into protein, as well as inhibition of biofilm formation (PubMed:24097941). Partially contradictory results are seen in another study where growth occurs in the presence of 20 and 500 mg/ml D-Trp but not in 500 mg/ml D-Asp or D-Ser; whether these are the exact same strains is unknown (PubMed:25441601). Strains in which this gene is restored by mutagenesis are resistant to D-Leu, D-Met, D-Trp and D-Tyr and form biofilm even in the presence of D-amino acids, while maintaining the ability to incorporate at least D-Trp into peptidoglycan (PubMed:24097941).
messages: Cytoplasm
messages: Homodimer.
messages: The dtd gene from B.amyloliquefaciens strain A50 confers the ability to grow in the presence of 20 mg/ml D-Tyr and 500 mg/ml D-Asp to B.subtilis, showing it can be used as a selective marker in bacteria that do not have a functional copy of this gene. Spontaneous resistance to D-Tyr is very rare, on the order of 10(-9) (PubMed:25441601).

224308:BSU 29880
gene id: 224308:BSU_29880
uniprot id: A0A6M3ZFM0
uniprot id: O34389
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the malic enzymes family.
messages: Catalyzes the decarboxylation of malate to pyruvate. Can use NAD and NADP, but with a strong preference for NAD. Can also catalyze the decarboxylation of oxaloacetate (PubMed:16788182). Involved in keeping the ATP levels high (PubMed:23136871).
messages: Divalent metal cations.
messages: Divalent metal cations. Prefers magnesium or manganese.
messages: Mutant can use either a gluconeogenic carbon source or glucose as efficiently as the wild-type strain (PubMed:16788182). The ATP concentrations in the mutant grown in minimal medium with glucose are similar to the wild-type level. ATP concentrations decrease by about 10% in malate minimal medium. The mleA-maeA-malS triple mutant shows a decrease in ATP concentrations by about 20% and a moderate growth defect (PubMed:23136871). NADPH overproduction is roughly halved in the deletion mutant (PubMed:33824210).
messages: Weakly expressed in glucose or malate minimal medium.

224308:BSU 20060
gene id: 224308:BSU_20060
uniprot id: O31875
names: Activity Regulation
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the ribonucleoside diphosphate reductase large chain family.
messages: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
messages: Tetramer of two alpha and two beta subunits.
messages: This protein undergoes protein self-splicing that involves post-translational excision of the intervening region (intein) followed by peptide ligation.
messages: Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).

224308:BSU 04870
gene id: 224308:BSU_04870
uniprot id: A0A6M3Z8A8
uniprot id: P96635
names: Function
names: Miscellaneous
names: Similarity
messages: Belongs to the plasmid replication initiation factor family.
messages: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation of a single strand of ICEBs1 DNA through a transmembrane conjugation pore into the recipient cell.
messages: The oriT nick site is located within the nicK open reading frame.

224308:BSU 29720
gene id: 224308:BSU_29720
uniprot id: A0A6M4JM04
uniprot id: P39064
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MotB family.
messages: Cell membrane
messages: May be involved in some transport function.

224308:BSU 00250
gene id: 224308:BSU_00250
uniprot id: P37467
names: Function
messages: In double copy it causes aberrant cell morphology, filamentation and inhibits sporulation. Hydrolyzes 5-bromo-4-chloroindolyl phosphate.

224308:BSU 25900
gene id: 224308:BSU_25900
uniprot id: P24808
names: Function
names: Similarity
messages: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
messages: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

224308:BSU 30350
gene id: 224308:BSU_30350
uniprot id: A0A6M4JML4
uniprot id: O34546
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 00280
gene id: 224308:BSU_00280
uniprot id: A0A6M3Z6C6
uniprot id: P37537
names: Function
names: Similarity
messages: Belongs to the thymidylate kinase family.
messages: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.

224308:BSU 28710
gene id: 224308:BSU_28710
uniprot id: A0A6M3ZJU8
uniprot id: P94532
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptide transporter carbon starvation (CstA) (TC 2.A.114) family.
messages: Cell membrane
messages: Involved in peptide utilization.
messages: Membrane

224308:BSU 05010
gene id: 224308:BSU_05010
uniprot id: P96649
names: Activity Regulation
names: Domain
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Activates ICEBs1 gene expression, excision and transfer by inactivating the ICEBs1 repressor protein ImmR (PubMed:16105942, PubMed:21036995). RapI-mediated induction likely results from an increase in the specific activity of the protease ImmA, which mediates proteolysis of ImmR (PubMed:21036995). In addition, is involved in regulation of sporulation (PubMed:23526881). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:23526881).
messages: Belongs to the Rap family.
messages: Contains a small N-terminal 3-helix bundle domain and a large C-terminal TPR domain, connected by a linker region.
messages: Cytoplasm
messages: Encoded by the mobile genetic element ICEBs1.
messages: Expression is stimulated by conditions of low nutrient availability and high cell density.
messages: Inhibited by PhrI.

224308:BSU 15490
gene id: 224308:BSU_15490
uniprot id: A0A6M4JGJ0
uniprot id: P05654
names: Similarity
names: Subunit
messages: Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.
messages: Homotrimer.

224308:BSU 32540
gene id: 224308:BSU_32540
uniprot id: O32150
names: Function
names: Subcellular Location Annotation
messages: Mg(2+)-activated ribonuclease which hydrolyzes RNA apparently nonspecifically into oligonucleotides with 5'-terminal phosphate.
messages: Secreted

224308:BSU 01650
gene id: 224308:BSU_01650
uniprot id: P40407
names: Disruption Phenotype
names: Domain
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the glycoside hydrolase 171 family.
messages: Catalyzes the exo-lytic cleavage of beta-1,4-N-acetylmuramate (beta-1,4-MurNAc) from the non-reducing ends of peptidoglycan chains (PubMed:33684445). Specifically hydrolyzes the natural, peptidoglycan-derived disaccharide MurNAc-GlcNAc and the artificial substrate para-nitrophenyl beta-N-acetylmuramic acid (pNP-MurNAc) (PubMed:33684445). Requires a MurNAc entity at the non-reducing end, and cannot cleave GlcNAc-MurNAc (PubMed:33684445). Probably plays a role in cell wall turnover and recycling (PubMed:33684445).
messages: Contains an N-terminal catalytic domain and a C-terminal auxiliary domain (PubMed:33684445). A putative active site is located in a cleft within the interface of two subdomains (PubMed:33684445).
messages: Deletion mutant accumulates specific cell wall fragments and shows growth defects under starvation conditions.
messages: Homodimer in solution.
messages: Secreted

224308:BSU 22770
gene id: 224308:BSU_22770
uniprot id: A3F3E0
uniprot id: P19466
names: Function
names: Similarity
names: Subunit
messages: Belongs to the MtrB family.
messages: Oligomer of 11 identical subunits arranged in doughnut-like structure.
messages: Required for transcription attenuation control in the Trp operon. This trans-acting factor seems to recognize a 10 bases nucleotide sequence in the Trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.
messages: Required for transcription attenuation control in the trp operon. This trans-acting factor binds to trinucleotide repeats (GAG or UAG) located in the trp leader transcript causing transcription termination. Binds the leader RNA only in presence of L-tryptophan.

224308:BSU 06550
gene id: 224308:BSU_06550
uniprot id: A0A6M4JDQ7
uniprot id: O34560
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 19180
gene id: 224308:BSU_19180
uniprot id: O34653
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the fatty acid desaturase type 1 family.
messages: Catalyzes the introduction of a cis-double bond at the delta(5) position of existing saturated fatty acids attached to membrane phospholipids. It is not strictly specific for palmitic acid (C16) but can also accept C14 as well as C18 species to yield unsaturated fatty acids.
messages: Cell membrane
messages: Transcriptionally regulated by DesR/DesK in response to cold shock.

224308:BSU 27720
gene id: 224308:BSU_27720
uniprot id: A0A6M3ZJN1
uniprot id: O32054
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the QueA family.
messages: Cytoplasm
messages: Monomer.
messages: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).

224308:BSU 29360
gene id: 224308:BSU_29360
uniprot id: A0A6M3ZFF6
uniprot id: O34315
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Part of the ABC transporter complex TcyJKLMN involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable). Is also involved in cystathionine, djenkolate, and S-methylcysteine transport.
messages: The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL and TcyM) and two solute-binding proteins (TcyJ and TcyK).

224308:BSU 15120
gene id: 224308:BSU_15120
uniprot id: A0A6M3ZAH8
uniprot id: P55342
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the BshC family.
messages: Homodimer in solution.
messages: Involved in bacillithiol (BSH) biosynthesis. May catalyze the last step of the pathway, the addition of cysteine to glucosamine malate (GlcN-Mal) to generate BSH.
messages: Mutant accumulates GlcN-Mal and does not produce BSH.

224308:BSU 40230
gene id: 224308:BSU_40230
uniprot id: A0A6M3ZI42
uniprot id: Q45601
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RNA methyltransferase RlmH family.
messages: Cytoplasm
messages: Homodimer.
messages: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.

224308:BSU 24790
gene id: 224308:BSU_24790
uniprot id: P54501
names: Cofactor
names: Similarity
messages: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
messages: Binds 2 Zn(2+) ions per subunit.

224308:BSU 39120
gene id: 224308:BSU_39120
uniprot id: P42304
names: Similarity
messages: Belongs to the 'GDSL' lipolytic enzyme family.

224308:BSU 13450
gene id: 224308:BSU_13450
uniprot id: A0A6M3ZAC8
uniprot id: O31654
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the sigma-70 factor family. SigI subfamily.
messages: Cells lacking this gene exhibit normal cell morphology and growth rate at 37 degrees Celsius, but cannot grow at high temperature.
messages: Cytoplasm
messages: Interacts with RsgI.
messages: Negatively regulated by the anti-sigma-I factor RsgI.
messages: Negatively regulated by the anti-sigma-I factor RsgI. Upon exposure to heat, SigI is released from RsgI and activated. Transient heat activation of SigI may depend upon DnaK chaperone.
messages: Part of the sigI-rsgI operon, which is transiently induced by heat stress. Expression is positively autoregulated via the sigma-I promoter (PubMed:11157964, PubMed:17185538). In exponentially growing cells, expression is regulated by the WalRK two-component system, which represses the sigma-I promoter and activates the sigma-A promoter, leading to the formation of a basal level of SigI (PubMed:23199363). WalRK can also positively and directly regulate transcription of the operon under heat stress through a binding site located upstream of the sigma-I promoter (PubMed:24125693). Repressed by glucose (PubMed:17185538).
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of cell wall metabolism in response to heat stress. Acts by regulating the expression of genes such as bcrC, mreBH and lytE (PubMed:18156261, PubMed:21541672, PubMed:23199363). Also plays a role in survival at low temperatures (PubMed:19114499).

224308:BSU 28390
gene id: 224308:BSU_28390
uniprot id: A0A6M3ZI35
uniprot id: P94556
names: Function
names: Similarity
messages: Belongs to the aspartate/glutamate racemases family.
messages: Provides the (R)-glutamate required for cell wall biosynthesis.

224308:BSU 38310
gene id: 224308:BSU_38310
uniprot id: A0A6M3ZHB5
uniprot id: P39592
names: Similarity
messages: Belongs to the LysR transcriptional regulatory family.

224308:BSU 17620
gene id: 224308:BSU_17620
uniprot id: P94492
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the thermonuclease family.
messages: Binds 1 Ca(2+) ion per subunit.
messages: Cell membrane
messages: Cells lacking this gene show DNase activity when exposed to thermal stress. Also shows an increased ability of competence.
messages: Shows DNase activity on double strand DNA.

224308:BSU 00740
gene id: 224308:BSU_00740
uniprot id: P28820
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the anthranilate synthase component I family.
messages: Monomer. Heterodimer consisting of two non-identical subunits: a glutamine amidotransferase subunit (PabA) and a aminodeoxychorismate synthase subunit (PabB).
messages: Part of a heterodimeric complex that catalyzes the two-step biosynthesis of 4-amino-4-deoxychorismate (ADC), a precursor of p-aminobenzoate (PABA) and tetrahydrofolate. In the first step, a glutamine amidotransferase (PabA) generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by aminodeoxychorismate synthase (PabB) to produce ADC.
messages: Requires p-aminobenzoic acid but not tryptophan for growth.
messages: The catalytically active amino acid residue K274 of E.coli enzyme is missing and corresponds to A283 in B.subtilis. It is postulated that the enzymatic mechanism for the PABA biosynthesis in B.subtilis proceeds without covalent intermediate. First, ammonia is added at C2 of chorismate with concomitant loss of the C4 hydroxy group, yielding 2-amino-2-deoxyisochorismate (ADIC). The second step is the addition of a second molecule ammonia to C4 of ADIC with concomitant loss of the C2 amino group, yielding ADC. Both steps are catalyzed by ADCS (PubMed:19275258).

224308:BSU 19630
gene id: 224308:BSU_19630
uniprot id: A0A6M4JKW7
uniprot id: O34925
names: Function
names: Similarity
names: Subunit
messages: Belongs to the PNP/UDP phosphorylase family.
messages: Catalyzes the reversible phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
messages: Homohexamer; trimer of homodimers.

224308:BSU 10020
gene id: 224308:BSU_10020
uniprot id: A0A6M3Z920
uniprot id: P80862
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: According to PubMed:16233211, SerC is not involved in pyridoxine biosynthesis in B.subtilis. In this organism, pyridoxal phosphate biosynthesis is achieved via an alternative pathway involving PdxS and PdxT.
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.
messages: Binds 1 pyridoxal phosphate per subunit.
messages: Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
messages: Cytoplasm
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 29239
gene id: 224308:BSU_29239
uniprot id: C0H3P8
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Forespore outer membrane
messages: Impaired ability to form spores when associated with a ytrI deletion. In combination with a ybaN deletion, cells show a severe sporulation defect.
messages: Involved in sporulation. May contribute to cortex formation or stability.
messages: Transcriptionally regulated by sigma-E.

224308:BSU 00310
gene id: 224308:BSU_00310
uniprot id: P37540
names: Function
names: Subunit
messages: DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).
messages: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria.

224308:BSU 28920
gene id: 224308:BSU_28920
uniprot id: P94514
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system LytS/LytT that probably regulates genes involved in cell wall metabolism.
messages: Phosphorylated by LytS.

224308:BSU 15890
gene id: 224308:BSU_15890
uniprot id: A0A6M3ZE81
uniprot id: P71018
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PlsX family.
messages: Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.
messages: Cytoplasm
messages: Homodimer (By similarity). Probably interacts with PlsY.
messages: Homodimer. Probably interacts with PlsY.

224308:BSU 33170
gene id: 224308:BSU_33170
uniprot id: A0A6M4JP37
uniprot id: O34451
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.
messages: Cell membrane
messages: Membrane
messages: Probably part of an ABC transporter complex. Probably responsible for the translocation of the substrate across the membrane (By similarity).
messages: The complex is composed of two ATP-binding proteins (YvrA), two transmembrane proteins (YvrB) and a solute-binding protein (YvrC).

224308:BSU 38360
gene id: 224308:BSU_38360
uniprot id: P39587
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the methyltransferase superfamily. RlmI family.
messages: Cytoplasm

224308:BSU 13880
gene id: 224308:BSU_13880
uniprot id: O31691
names: Domain
names: Function
names: PTM
names: Similarity
names: Subunit
messages: Belongs to the transcriptional antiterminator BglG family. GlcT subfamily.
messages: Composed of 3 domains: an N-terminal RNA-binding domain that prevents transcriptional termination, and two PTS regulation domains, PRD 1 and PRD 2. PRD 1 is the target of negative control exerted by EII-Glc and PRD 2 is the target of HPr.
messages: Homodimer (By similarity). The monomeric form probably also exists but it would be inactive in RNA binding and antitermination.
messages: Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence.
messages: Phosphorylated by HPr (PtsH) and EII-Glc (PtsG). HPr phosphorylates the PRD 2 domain which has a slight stimulatory effect on GlcT activity, while EII-Glc phosphorylates the PRD 1 domain which inactivates GlcT. The phosphorylation is dependent on the presence or absence of glucose which acts as an inducer of the ptsGHI operon expression. In the presence of glucose the phosphoryl group is transferred from phosphorylated HPr to the sugar via EII-Glc. Under these conditions GlcT is not phosphorylated and binds to the RAT sequence, thus allowing transcription of the ptsGHI operon. In the absence of glucose, phosphorylated EII-Glc accumulates in the cell and phosphorylates the PRD 1 domain of GlcT, leading to its inactivation; this phosphorylation may prevent dimerization of GlcT.

224308:BSU 04680
gene id: 224308:BSU_04680
uniprot id: P42410
names: Function
names: PTM
names: Subunit
messages: Interacts with RsbRA, RsbRB in the stressosome. The stressosome probably also contains RsbRC and RsbRD.
messages: Negative regulator of sigma-B activity. Non-phosphorylated RsbS binds to RsbT, preventing its association with RsbU. Requires any one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and the RsbR paralog(s) are phosphorylated, they release RsbT, which can then bind and activate RsbU.
messages: Phosphorylated by RsbT. Dephosphorylated by RsbX.

224308:BSU 20560
gene id: 224308:BSU_20560
uniprot id: O31923
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02360
gene id: 224308:BSU_02360
uniprot id: A0A6M4JI37
uniprot id: O31458
names: Caution
names: Function
names: Induction
names: Similarity
messages: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.
messages: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.
messages: Expression is repressed by the HTH-type transcriptional regulator GamR.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 13570
gene id: 224308:BSU_13570
uniprot id: O31664
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
messages: Impaired growth on methylthioribose (MTR) as sole sulfur source.
messages: Involved in the methylthioribose (MTR) recycling pathway (PubMed:12022921, PubMed:24837359). Probably catalyzes the conversion of 2-oxoglutaramate to 2-oxoglutarate (PubMed:24837359).

224308:BSU 22460
gene id: 224308:BSU_22460
uniprot id: A0A6M3ZDL5
uniprot id: P42982
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
messages: Homotetramer.
messages: Inhibited by BSH.
messages: Involved in bacillithiol (BSH) biosynthesis. Catalyzes the first step of the pathway, the formation of N-acetylglucosaminylmalate (GlcNAc-Mal) from UDP-N-acetylglucosamine (UDP-GlcNAc) and L-malate.
messages: Mutant does not produce bacillithiol.

224308:BSU 33010
gene id: 224308:BSU_33010
uniprot id: A0A6M3ZKR2
uniprot id: O32192
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress.
messages: Phosphorylated by CssS.

224308:BSU 12671
gene id: 224308:BSU_12671
uniprot id: P54333
names: Similarity
messages: To B.subtilis YqbN.

224308:BSU 10960
gene id: 224308:BSU_10960
uniprot id: A0A6M3Z9A8
uniprot id: O06740
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Membrane

224308:BSU 33510
gene id: 224308:BSU_33510
uniprot id: O32221
names: Function
names: Induction
names: Miscellaneous
names: Subcellular Location Annotation
names: Subunit
messages: By Cu(2+).
messages: Chaperone that serves for the intracellular sequestration and transport of Cu(+). Delivers Cu(+) to the copper-transporting ATPase CopA. Functions in E.coli to transfer Cu(+) to CopA missing its first metal-binding domain (PubMed:25899340).
messages: Cu(+) ion is always at least three-coordinated. Physiological thiol may be needed to complete the Cu(+) coordination sphere in order to prevent homodimer (dead-end products) formation between 2 CopZ.
messages: Cytoplasm
messages: Monomer in the absence of copper. Homodimer in the presence of copper ions. Forms a heterodimer (electrostatic interactions) with CopA during the transfer of Cu(+).
messages: The copZA operon is activated by CueR and indirectly repressed by YfmP.

224308:BSU 25270
gene id: 224308:BSU_25270
uniprot id: A0A6M3ZEJ6
uniprot id: P54380
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Tetramer of two alpha and two beta subunits.

224308:BSU 35930
gene id: 224308:BSU_35930
uniprot id: A0A6M3ZGX1
uniprot id: P36946
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RbsD / FucU family. RbsD subfamily.
messages: Catalyzes the interconversion of beta-pyran and beta-furan forms of D-ribose.
messages: Cytoplasm
messages: Homodecamer.
messages: Homodecamer; dimer of pentamers.
messages: Was originally thought (PubMed:7921236, PubMed:9353933) to be a high affinity ribose transport protein.

224308:BSU 10810
gene id: 224308:BSU_10810
uniprot id: O06728
names: Activity Regulation
names: Caution
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: A farnesyl diphosphate (FPP) phosphatase. Involved in biofilm formation, its disruption blocks biofilm synthesis which is restored by exogenous farnesol (PubMed:25308276). Releases diphosphate from FPP, was initally suggested to be a squalene synthase. Diphosphate release is higher from FPP than geranyl pyrophosphate (GPP) or geranylgeranyl pyrophosphate (GGPP). Biofilm synthesis is partially restored by exogenous squalene, beta-carotene or retinol. Required for integrity of cell membrane lipid rafts (PubMed:20713508). Involved in spatial organization of membranes, required for the flotillin-like proteins FloT and FloA to function correctly (PubMed:23651456).
messages: Belongs to the phytoene/squalene synthase family.
messages: Diphosphate release from FPP is inhibited by zaragozic acid.
messages: Loss of biofilm formation (PubMed:20713508, PubMed:25308276). Loss of a dark-orange pigment from cells, alteration of cellular lipid profile. Significant reduction of proteins found in detergent-resistant membrane (DRM) fractions (PubMed:20713508). Decrease in membrane fluidity, about 30% decrease in protein secretion (PubMed:23651456).
messages: Monomer.
messages: Was originally thought to be a squalene synthase (PubMed:20713508). Further characterization suggests it is actually a farnesyl diphosphate phosphatase (PubMed:25308276).

224308:BSU 12070
gene id: 224308:BSU_12070
uniprot id: O31651
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 34970
gene id: 224308:BSU_34970
uniprot id: A0A6M4JPJ5
uniprot id: Q9JMQ2
names: Caution
names: Function
names: Similarity
messages: Belongs to the HAD-like hydrolase superfamily. PpaX family.
messages: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool.
messages: Was originally (PubMed:9465101) called P-Ser-HPr phosphatase as it has been shown to stimulate P-Ser-HPr dephosphorylation, but actually (PubMed:12359880) it has pyrophosphatase activity.

224308:BSU 33410
gene id: 224308:BSU_33410
uniprot id: O32211
names: Induction
messages: By phosphate starvation, via the alternative sigma factor sigma-B.

224308:BSU 35710
gene id: 224308:BSU_35710
uniprot id: A0A6M3ZGY4
uniprot id: P42953
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC-2 integral membrane protein family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex TagGH (TC 3.A.1.34.1) involved in exporting the two types of intracellularly synthesized teichoic acids. Probably responsible for the translocation of the substrate across the membrane.
messages: The complex is composed of two ATP-binding proteins (TagH) and two transmembrane proteins (TagG).

224308:BSU 01350
gene id: 224308:BSU_01350
uniprot id: A0A6M4JET5
uniprot id: P19946
names: Function
names: Similarity
names: Subunit
messages: Belongs to the universal ribosomal protein uL15 family.
messages: Binds to the 23S rRNA.
messages: Part of the 50S ribosomal subunit.

224308:BSU 35700
gene id: 224308:BSU_35700
uniprot id: A0A6M4JPR5
uniprot id: P42954
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Belongs to the ABC transporter superfamily. Teichoic acids exporter (TC 3.A.1.104.1) family.
messages: Cell membrane
messages: Part of the ABC transporter complex TagGH involved in teichoic acids export. Responsible for energy coupling to the transport system.
messages: The complex is composed of two ATP-binding proteins (TagH) and two transmembrane proteins (TagG).

224308:BSU 07860
gene id: 224308:BSU_07860
uniprot id: A0A6M4JEA9
uniprot id: O34597
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 07340
gene id: 224308:BSU_07340
uniprot id: A0A6M4JKZ7
uniprot id: Q797A7
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Disruption of the gene leads to almost full resistance to trifluoromethylthioribose (3FMTR), a toxic analog of MTR.
messages: Involved in import of methylthioribose (MTR) into the cell.
messages: Membrane

224308:BSU 37180
gene id: 224308:BSU_37180
uniprot id: P45866
names: Cofactor
names: Induction
names: Subcellular Location Annotation
messages: Binds 2 [4Fe-4S] clusters.
messages: Cell membrane
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to FadR boxes on target DNA and thus derepress transcription.

224308:BSU 31300
gene id: 224308:BSU_31300
uniprot id: A0A6M4JKY1
uniprot id: O05241
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0053 family.
messages: Cell membrane
messages: Membrane

224308:BSU 33730
gene id: 224308:BSU_33730
uniprot id: A0A6M4JP91
uniprot id: Q45460
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the ABC transporter superfamily.
messages: Involved in a high affinity multicomponent binding-protein-dependent transport system for choline. Probably responsible for energy coupling to the transport system.
messages: LH45 is not a natural strain. It has been constructed by transformation of strain 168 with linear DNA from B.subtilis ATCC 6633. It is a subtilin-producing mutant of B.subtilis 168 (PubMed:7592481).
messages: Repressed by GbsR.

224308:BSU 00240
gene id: 224308:BSU_00240
uniprot id: P37534
names: Cofactor
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Subunit
messages: An anti-sigma-G factor, prevents premature activation of sigma-G factor in the forespore; overexpression leads to 1000-fold reduction in spore formation, spore formation stops after engulfment (PubMed:17921305, PubMed:19497328). Overexpression also inhibits sigma-G transcription activation activity (PubMed:18208527). When both Gin and sigma-G are expressed in E.coli Gin inhibits sigma-G, strongly suggesting Gin inhibits by direct physical interaction (PubMed:19497328).
messages: Binds 0.5 mol of zinc/mol protein, probably 1 zinc per dimer.
messages: During sporulation under control of sigma-F factor.
messages: Expressed starting 2 hours after sporulation onset for at least 7 hours.
messages: Premature expression of sigma-G factor (sigG or spoIIIG) activity during the early stages of forespore development (PubMed:17921305, PubMed:18208527). Spore formation continues normally (PubMed:17921305, PubMed:8759874). However its absence has deleterious effects on strain robustness and is strongly selected against in competitions experiments (PubMed:18208527).
messages: Probably functions as a homodimer (PubMed:19497328). Interacts with sigma-G factor, recognition occurs via the first 71 residues of sigma-G (PubMed:18208527, PubMed:19497328).

224308:BSU 26840
gene id: 224308:BSU_26840
uniprot id: A0A6M3ZEZ8
uniprot id: O05409
names: Function
names: Similarity
messages: Belongs to the sigma-70 factor family. ECF subfamily.
messages: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.

224308:BSU 09250
gene id: 224308:BSU_09250
uniprot id: A0A6M3Z8V7
uniprot id: P54608
names: Disruption Phenotype
names: Similarity
messages: Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
messages: No visible growth phenotype.

224308:BSU 29250
gene id: 224308:BSU_29250
uniprot id: O34600
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the NrnA oligoribonuclease family.
messages: Bifunctional enzyme which has both oligoribonuclease and pAp-phosphatase activities. Degrades RNA and DNA oligonucleotides with a length of 5 nucleotides and shorter, with a preference for 3-mers. Directionality is controversial; shown to degrade 5-mers and less in a 3' to 5' direction (PubMed:17586819), and 11-mers in a 5' to 3' direction (PubMed:21087930). Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.
messages: In accordance with its dual activities, is able to complement both orn and cysQ mutants in E.coli.
messages: Tetramer.

224308:BSU 12870
gene id: 224308:BSU_12870
uniprot id: O34689
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the extradiol ring-cleavage dioxygenase family.
messages: Binds 1 Fe(2+) ion.
messages: Cytoplasm
messages: Putative ring-cleavage dioxygenase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 11670
gene id: 224308:BSU_11670
uniprot id: O31616
names: Activity Regulation
names: Biotechnology
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the DAO family. ThiO subfamily.
messages: Binds 1 FAD per subunit.
messages: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine (PubMed:9827558, PubMed:11744710, PubMed:19864430). Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine) (PubMed:19864430). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine (PubMed:9827558, PubMed:11744710). Does not act on L-amino acids and other D-amino acids (PubMed:9827558). Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate (PubMed:12627963).
messages: Cells lacking this gene have an absolute requirement for the thiazole alcohol for growth.
messages: Cytoplasm
messages: Homotetramer.
messages: Introducing the gene coding for the glycine oxidase mutant Ser-51/Arg-54/Ala-244 in plants may be an effective alternative mechanism for glyphosate tolerance in transgenic crops. In addition, transgenic plants that are able to oxidize glyphosate may represent an innovative bioremediation system for the soil treated with this herbicide.
messages: Is competitively inhibited by glycolate.

224308:BSU 13440
gene id: 224308:BSU_13440
uniprot id: A0A6M3ZAK3
uniprot id: O34997
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TerC family.
messages: Cell membrane

224308:BSU 22030
gene id: 224308:BSU_22030
uniprot id: P54159
names: Cofactor
names: Disruption Phenotype
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
messages: Cell membrane
messages: Has 2 similar domains, D1 (approximately residues 1-600) and D2 (approximately residues 601-1193). D1 is able to bind membranes, tether liposomes and cause membrane fusion in the absence of D2; both regions are required for GTPase activity. Possibly the fusion of 2 dynamin-like genes (PubMed:21205012). D1 alone promotes membrane tethering but requires D2 for stable tethering and content mixing (PubMed:31361971).
messages: Has intrinsic affinity for membranes and membrane distortion capability; causes tubulation and membrane distortion when expressed in a Drosophila cell line.
messages: Homodimer in solution (Probable). Both D1 and D2 domains interact with YwpG, YneK interacts only with D1 while RNase Y (rny) only interacts with whole protein (PubMed:23060960). Probably oligomerizes at damaged membrane sites (Probable).
messages: Mediates lipid mixing of vesicles and full mixing of their contents in the absence and presence of GTP. Tethers and mixes small vesicles better than larger ones, indicating a curvature preference. GTP slows down DynA-mediated lipid fusion, perhaps controlling its activity. Prefers phospholipid composition close to the B.subtilis membrane; requires phosphatidylglycerol for fusion has no activity on pure phosphatidylethanolamine vesicles (PubMed:31361971). Regulates membrane lipid diffusion. Required to prevent membrane damage when exposed to low levels of membrane-damaging antibiotics or to bacteriophage. Probably surveys the cell membrane for stress; localizes to sites of membrane damage (treatment with nisin) and forms foci in cells treated with pore-forming compounds (CCCP). May assist membrane repair, possibly by membrane tethering and fusion (PubMed:26530236). Probably functions both in early and late cell division, affects the proper formation of the FtsZ ring. Plays a non-redundant role with flottilin (floT) in membrane dynamics and cell shape. Probably able to bend membranes (PubMed:23249255). Tethers liposomes and mediates their fusion; this does not require GTPase activity or the presence of GTP. Both GTPase domains (dynamin-type G) are required for GTPase activity (PubMed:21205012).
messages: No visible phenotype (PubMed:21205012). 5% of cells have a double septa, 2% of cells are very long. Double dynA-ezrA mutants have longer cells with more double septa than either deletion alone. Double dynA-floT deletions are highly elongated, filamentous and have strong defects in cell shape; cells grow very slowly with an extended lag phase. Double dynA-mreB deletions have strong cell shape defects (PubMed:23249255). Double dynA-floT deletions are less motile than single floT deletions (PubMed:26842743). Increased lipid mobility in the cell membrane. Severe decrease in growth on nisin (causes membrane pore formation) with significant membrane deformation and damage, intermediate decrease in growth on antibiotics acting on lipid II or the membrane (bacitracin and daptomycin). No effect on growth on vancomycin (blocks peptidoglycan cross-links) or gramicidin D (makes small pores). 20-50% increased susceptibility to phage phi29 and SPbeta (PubMed:26530236).
messages: Requires Mg(2+) for membrane fusion.
messages: Strain PSB025 / 25152 does not have the SP-beta prophage.

224308:BSU 33430
gene id: 224308:BSU_33430
uniprot id: A0A6M4JP58
uniprot id: O32213
names: Cofactor
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).
messages: Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein.
messages: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Binds 1 siroheme per subunit.
messages: Cells lacking the cysIJ genes are unable to use sulfate, sulfite or butanesulfonate as sole sulfur source, grow poorly with sulfide, but can still grow with thiosulfate, cysteine or methionine.
messages: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (Probable).
messages: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.
messages: Up-regulated by sulfate and the transcriptional regulator CysL.

224308:BSU 27320
gene id: 224308:BSU_27320
uniprot id: A0A6M4JLZ2
uniprot id: P80240
names: Function
names: Induction
names: Similarity
messages: Belongs to the GreA/GreB family.
messages: By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation.
messages: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides (By similarity).
messages: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.

224308:BSU 06730
gene id: 224308:BSU_06730
uniprot id: A0A6M3Z867
uniprot id: O31503
names: Function
names: Similarity
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.
messages: Catalyzes the formation of 5-methyl-uridine at positions 747 (m5U747) and 1939 (m5U1939) in 23S rRNA.

224308:BSU 01400
gene id: 224308:BSU_01400
uniprot id: A0A6M3Z6W0
uniprot id: P20278
names: Similarity
names: Subunit
messages: Belongs to the bacterial ribosomal protein bL36 family.
messages: Part of the 50S ribosomal subunit.

224308:BSU 03050
gene id: 224308:BSU_03050
uniprot id: A0A6M4JIA2
uniprot id: P13714
names: Activity Regulation
names: Caution
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Allosterically activated by fructose 1,6-bisphosphate (FBP).
messages: Belongs to the LDH/MDH superfamily. LDH family.
messages: Catalyzes the conversion of lactate to pyruvate.
messages: Cytoplasm
messages: Homotetramer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM.

224308:BSU 07110
gene id: 224308:BSU_07110
uniprot id: A0A6M4JE53
uniprot id: P39128
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 09690
gene id: 224308:BSU_09690
uniprot id: A0A6M4JEE2
uniprot id: O07552
names: Induction
names: Similarity
messages: Belongs to the universal stress protein A family.
messages: By phosphate starvation, via the alternative sigma factor sigma-B.

224308:BSU 38860
gene id: 224308:BSU_38860
uniprot id: A0A6M4JQM3
uniprot id: P55180
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NAD(P)-dependent epimerase/dehydratase family.
messages: Homodimer.
messages: Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD (By similarity).

224308:BSU 15040
gene id: 224308:BSU_15040
uniprot id: A0A6M4JIC4
uniprot id: O34731
names: Caution
names: Similarity
messages: Belongs to the NTE family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 10340
gene id: 224308:BSU_10340
uniprot id: O07617
names: Caution
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the phosphoglycerate mutase family. GpmB subfamily.
messages: In contrast to other members of the family, has no phosphoglycerate mutase activity.
messages: No visible phenotype.
messages: Phosphatase with broad substrate specificity. Does not have phosphoglycerate mutase activity.

224308:BSU 08425
gene id: 224308:BSU_08425
uniprot id: A0A6M4JG44
uniprot id: C0H3X7
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the LPG synthase family.
messages: Catalyzes the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), a major component of the bacterial membrane with a positive net charge. LPG synthesis contributes to bacterial virulence as it is involved in the resistance mechanism against cationic antimicrobial peptides (CAMP) produces by the host's immune system (defensins, cathelicidins) and by the competing microorganisms.
messages: Catalyzes the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), one of the components of the bacterial membrane with a positive net charge. LPG synthesis contributes to the resistance to cationic antimicrobial peptides (CAMPs) and likely protects B.subtilis against its own CAMPs and against those produced by competiting microorganisms (bacteriocins). In fact, the modification of anionic phosphatidylglycerol with positively charged L-lysine results in repulsion of the peptides.
messages: Cell membrane
messages: Cells lacking this gene lack lysyl-phosphatidylglycerol in their membranes. They also display an increased sensitivity to the antibiotics nisin and daptomycin (PubMed:18820022, PubMed:19164152). Cells appear normal, no effect on flotillin cluster numbers or size (PubMed:27362352).
messages: Membrane

224308:BSU 22450
gene id: 224308:BSU_22450
uniprot id: A0A6M4JK56
uniprot id: P42977
names: Caution
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: A single active site specifically recognizes both ATP and CTP and is responsible for their addition.
messages: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.
messages: Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Has no poly(A) polymerase activity.
messages: Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.
messages: Homodimer.
messages: Was originally predicted to have poly(A) polymerase activity (EC 2.7.7.19), and its gene was named papS.

224308:BSU 07250
gene id: 224308:BSU_07250
uniprot id: A0A6M3ZBL7
uniprot id: O08394
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Functions as a fatty acid monooxygenase.
messages: Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of a range of long-chain fatty acids, with a preference for long-chain unsaturated and branched-chain fatty acids over saturated fatty acids. Hydroxylation of myristic acid occurs mainly at the omega-2 position. Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:15375636, PubMed:15122913). Is also able to catalyze efficient oxidation of sodium dodecyl sulfate (SDS) (PubMed:21048857).
messages: In the N-terminal section; belongs to the cytochrome P450 family.

224308:BSU 32220
gene id: 224308:BSU_32220
uniprot id: O32119
names: Function
names: Similarity
messages: Belongs to the NifU family.
messages: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins.

224308:BSU 32840
gene id: 224308:BSU_32840
uniprot id: O32178
names: Function
names: Induction
names: Similarity
messages: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
messages: Involved in the degradation of long-chain fatty acids.
messages: Repressed by FadR in the absence of LCFAs (fatty acids of 14-20 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs, which antagonize FadR as to its binding to fadR boxes on target DNA and thus derepress transcription.

224308:BSU 24510
gene id: 224308:BSU_24510
uniprot id: P54513
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Probable lipid hydrolase.

224308:BSU 15200
gene id: 224308:BSU_15200
uniprot id: A0A6M4JLL7
uniprot id: Q03522
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MurCDEF family.
messages: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).
messages: Cytoplasm

224308:BSU 33750
gene id: 224308:BSU_33750
uniprot id: O34889
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
messages: By Spo0A during nutrient starvation (PubMed:12817086). Repressed by AbrB during regular growth when nutrients are plentiful, in association with the transcriptional repressor Abh (PubMed:17720793).
messages: Cytoplasm
messages: Required for the maturation of SdpC to SDP (PubMed:12817086). Not required for SdpC signal peptide cleavage, secretion from the cell or disulfide bond formation (PubMed:23687264).
messages: When the sdpA-sdpB-sdpC operon is deleted, increased rate of spore formation; a double operon deletion (sdpA-sdpC plus skfA-skfH) makes spores even faster (PubMed:12817086). A single deletion mutant does not have SDP activity (active peptide of sdpC) (PubMed:23687264).

224308:BSU 24350
gene id: 224308:BSU_24350
uniprot id: A0A6M4JLN7
uniprot id: P49786
names: Function
names: Subunit
messages: Homodimer.
messages: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA (By similarity). Binds biotin (PubMed:7592499).
messages: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.

224308:BSU 03830
gene id: 224308:BSU_03830
uniprot id: P94421
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the bacterial solute-binding protein 8 family.
messages: Cell membrane
messages: Disruption mutants are unable to use petrobactin for iron delivery and growth.
messages: Membrane raft
messages: Part of the ABC transporter complex YclNOPQ involved in uptake of ferric-petrobactin. Petrobactin is a photoreactive 3,4-catecholate siderophore produced by many members of the B.cereus group, including B.anthracis. Binds selectively iron-free and ferric petrobactin and the petrobactin precursor 3,4-dihydroxybenzoic acid (3,4-DHB).
messages: The complex is composed of two ATP-binding proteins (YclP), two transmembrane proteins (YclN and YclO) and a solute-binding protein (YclQ) (Probable). Interacts with FloT (PubMed:23651456).

224308:BSU 16790
gene id: 224308:BSU_16790
uniprot id: Q99171
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S14 family. TepA subfamily.
messages: Cytoplasm
messages: Required for efficient translocation of pre-proteins across the membrane.

224308:BSU 37330
gene id: 224308:BSU_37330
uniprot id: A0A6M3ZH20
uniprot id: P46906
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-I aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Monomer.

224308:BSU 26430
gene id: 224308:BSU_26430
uniprot id: A0A6M3ZJC9
uniprot id: P54443
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YrkQ/YrkP.
messages: Phosphorylated by YrkQ.

224308:BSU 18770
gene id: 224308:BSU_18770
uniprot id: A0A6M4JMF9
uniprot id: O34416
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the EamA transporter family.
messages: Cell membrane

224308:BSU 32480
gene id: 224308:BSU_32480
uniprot id: O32144
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the xanthine dehydrogenase family.
messages: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.
messages: Could be composed of four subunits: PucA, PucC, PucD and PucE.
messages: Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression decreases when allantoin is added during limiting-nitrogen conditions.
messages: Oxidizes hypoxanthine and xanthine to uric acid.

224308:BSU 36060
gene id: 224308:BSU_36060
uniprot id: Q45535
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Assembly of CotH requires CotE and GerE.
messages: Belongs to the CotH family.
messages: Expression is negatively regulated by the transcriptional regulator GerE.
messages: Involved in the assembly of several proteins in the inner and outer layer of the spore coat. Stabilizes CotC and CotU in the mother cell compartment of sporulating cells and promotes the assembly of both early and late forms of CotC-related polypeptides on the spore surface.
messages: Spore coat

224308:BSU 16660
gene id: 224308:BSU_16660
uniprot id: A0A6M3ZAX8
uniprot id: P32732
names: Function
names: Similarity
messages: Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.
messages: Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.

224308:BSU 26600
gene id: 224308:BSU_26600
uniprot id: P39909
names: Activity Regulation
names: Function
names: Similarity
messages: Acetylates both spermidine and spermine at primary propyl amine moieties, with spermine being the preferred substrate.
messages: Belongs to the acetyltransferase family.
messages: Putrescine and N(8)-acetylspermidine are competitive inhibitors of spermidine acetylation.

224308:BSU 13410
gene id: 224308:BSU_13410
uniprot id: A0A6M3ZDH7
uniprot id: O34859
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the prokaryotic Ku family.
messages: Expressed in sporulating cells. Disappears after 90 minutes of spore germination.
messages: Homodimer. Interacts with LigD.
messages: Spore core
messages: Stationary-phase cells lacking this gene are more sensitive to ionizing radiation. Growth rate, ultraviolet light and methyl-methanesulfonate sensitivities are unaffected. The spores formed are significantly more sensitive to dry heat, high vacuum-induced desiccation, X-rays and UV radiation, but no difference in hydrogen peroxide resistance exists.
messages: Transcriptionally regulated by SpoVT and sigma-G factor.
messages: With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD (By similarity). Probably involved in DNA repair during spore germination.
messages: With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD.

224308:BSU 02910
gene id: 224308:BSU_02910
uniprot id: A0A6M3ZCE1
uniprot id: O34384
names: Similarity
messages: Belongs to the CAPAB/TerDEXZ family.

224308:BSU 10160
gene id: 224308:BSU_10160
uniprot id: P32399
names: Similarity
names: Subcellular Location Annotation
messages: Cell membrane
messages: To L.lactis phage infection protein (PIP).

224308:BSU 22050
gene id: 224308:BSU_22050
uniprot id: P54157
names: Similarity
messages: Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.

224308:BSU 37770
gene id: 224308:BSU_37770
uniprot id: P39635
names: Function
names: Induction
messages: By arginine.
messages: Involved in arginine degradative pathway.

224308:BSU 38660
gene id: 224308:BSU_38660
uniprot id: P94374
names: Induction
names: Similarity
messages: Belongs to the ABC transporter superfamily.
messages: Expression is sigma Y-dependent. Induced upon nitrogen starvation.

224308:BSU 14430
gene id: 224308:BSU_14430
uniprot id: O31716
names: Similarity
messages: Belongs to the ABC transporter superfamily.

224308:BSU 16630
gene id: 224308:BSU_16630
uniprot id: A0A6M3ZBI5
uniprot id: P17889
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.
messages: Cytoplasm
messages: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
messages: The alternative initiation site Met-94 is uncertain.

224308:BSU 02750
gene id: 224308:BSU_02750
uniprot id: P46903
names: Function
names: Induction
names: Similarity
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Induced by ethanol and the protonophore carbonylcyanide p-chlorophenylhydrazone (CCCP) and, more modestly, by sodium and potassium. Positively regulated by the two-component regulatory system NatK/NatR.
messages: Part of an ABC transporter that catalyzes ATP-dependent electrogenic sodium extrusion.
messages: The complex is composed of NatA and NatB.

224308:BSU 08120
gene id: 224308:BSU_08120
uniprot id: A0A6M4JGL2
uniprot id: O31553
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0060 family.
messages: Cell membrane

224308:BSU 32110
gene id: 224308:BSU_32110
uniprot id: A0A6M3ZFP1
uniprot id: O05268
names: Caution
names: Cofactor
names: Similarity
names: Subunit
messages: Belongs to the ferredoxin--NADP reductase type 2 family.
messages: Binds 1 FAD per subunit.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 06650
gene id: 224308:BSU_06650
uniprot id: A0A6M4JFP1
uniprot id: Q45514
names: Developmental Stage
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the MgtC/SapB family.
messages: Cell membrane
messages: Cells activate alkaline phosphatase during sporulation; the same phenotype is seen in the sapB2 and sapB10 mutants.
messages: Induced during late exponential phase, and maximum expression is reached during the first hour of stationary phase, both under sporulation and non-sporulation conditions.
messages: Membrane

224308:BSU 17980
gene id: 224308:BSU_17980
uniprot id: A0A6M3ZER3
uniprot id: P71032
names: Caution
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SspP family.
messages: Expressed only in the forespore compartment of sporulating cells.
messages: Expressed only in the forespore compartment of sporulating cells. Expression is sigma G-dependent.
messages: Spore core
messages: Was originally thought to be a spore coat protein.

224308:BSU 07300
gene id: 224308:BSU_07300
uniprot id: O06483
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.

224308:BSU 06830
gene id: 224308:BSU_06830
uniprot id: Q59HN8
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Rap family.
messages: Both activities are inhibited by RapH.
messages: Contains a small N-terminal 3-helix bundle domain and a large C-terminal TPR domain, connected by a linker region.
messages: Cytoplasm
messages: Deletion of the gene induces both differentiation pathways and interferes with their temporal separation.
messages: Dual specificity regulatory protein that can control both sporulation and competence by acting on two distinct response regulators: Spo0F and ComA, respectively (PubMed:17581123, PubMed:21346797). Is involved in the temporal separation of competence and sporulation (PubMed:17581123). Acts as a phosphatase that specifically dephosphorylates the sporulation initiation phosphotransferase Spo0F and inhibits its activity (PubMed:17581123, PubMed:21346797). RapH can also antagonize sporulation by sterically blocking phosphoryl transfer to and from Spo0F (PubMed:21346797). In addition, inhibits the activity of ComA, a transcriptional factor that regulates the development of genetic competence (PubMed:17581123). Acts by binding to ComA, leading to the inhibition of its DNA-binding activity (PubMed:17581123).
messages: Homodimer (PubMed:21346797). Interacts with phosphorylated Spo0F (PubMed:21346797). Each RapH protomer is bound to a monomer of Spo0F, forming an heterotetrameric complex (PubMed:21346797). May also interact with non-phosphorylated Spo0F to inhibit the sporulation phosphorelay (PubMed:21346797). Interacts with the C-terminal DNA-binding region of ComA (PubMed:17581123). Does not interact with DegU (PubMed:17581123).
messages: Part of the rapH-phrH operon, which is transcribed from the SigA-driven rapH promoter (PubMed:21908671). Activated by the late competence transcription factor ComK (PubMed:17581123). Repressed by RghR (PubMed:16553878, PubMed:17581123).

224308:BSU 36560
gene id: 224308:BSU_36560
uniprot id: P71043
names: Function
names: Similarity
messages: Belongs to the acetyltransferase family. PAT/BAR subfamily.
messages: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group (By similarity).

224308:BSU 38800
gene id: 224308:BSU_38800
uniprot id: P94361
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the polysaccharide deacetylase family.
messages: Cell membrane

224308:BSU 06900
gene id: 224308:BSU_06900
uniprot id: Q45537
names: Function
messages: The cotJ operon proteins affect spore coat composition. They are either required for the normal formation of the inner layers of the coat or are themselves structural components of the coat.

224308:BSU 12040
gene id: 224308:BSU_12040
uniprot id: O31648
names: Similarity
messages: Belongs to the acetyltransferase family.

224308:BSU 22380
gene id: 224308:BSU_22380
uniprot id: P54396
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 11510
gene id: 224308:BSU_11510
uniprot id: O31603
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the TerC family.
messages: Cell membrane

224308:BSU 28910
gene id: 224308:BSU_28910
uniprot id: A0A6M4JMJ5
uniprot id: P94515
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CidA/LrgA family. LrgA subfamily.
messages: Cell membrane
messages: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgB, with the holin-like protein CidA. The LrgAB and CidA proteins may affect the proton motive force of the membrane. May be involved in programmed cell death (PCD), possibly triggering PCD in response to antibiotics and environmental stresses.
messages: Membrane

224308:BSU 04670
gene id: 224308:BSU_04670
uniprot id: P42409
names: Disruption Phenotype
names: Function
names: PTM
names: Subunit
messages: Acts as a positive regulator of sigma-B activity in response to salt and heat stress by stimulating the activity of the RsbT kinase toward RsbS in vitro.
messages: Cells lacking this gene have a decreased response to salt stress, indicating that RsbRA is a positive regulator of sigma-B activity. Its activity is dependent on RsbRB.
messages: Interacts with RsbRB and RsbS in the stressosome. The stressosome probably also contains RsbRC and RsbRD.
messages: Negative regulator of sigma-B activity. Non-phosphorylated RsbS binds to RsbT, preventing its association with RsbU. Requires any one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and the RsbR paralog(s) are phosphorylated, they release RsbT, which can then bind and activate RsbU.
messages: One of 4 functionally non-identical RsbR paralogs, it functions in the environmental signaling branch of the general stress response.
messages: Phosphorylated by RsbT. This threonine phosphorylation abrogates the ability of RsbRA to stimulate RsbT in vitro.

224308:BSU 35590
gene id: 224308:BSU_35590
uniprot id: A0A6M3ZGI6
uniprot id: O32272
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
messages: By phosphate starvation, via the PhoP/PhoR two-component regulatory system.
messages: The nature of the anionic polymer present in the cell wall of B.subtilis depends on phosphate availability. Under phosphate-replete growth conditions teichoic acids are present, whereas under phosphate-depleted conditions, at least part of the wall teichoic acid is replaced with teichuronic acid, a non-phosphate containing anionic polymer. The synthesis of teichuronic acid is accompanied by degradation of teichoic acid and reutilization of liberated phosphate for other cellular processes such as nucleic acid synthesis.

224308:BSU 34020
gene id: 224308:BSU_34020
uniprot id: O32258
names: Similarity
messages: Belongs to the glycosyl hydrolase 18 family.

224308:BSU 16950
gene id: 224308:BSU_16950
uniprot id: O31773
names: Developmental Stage
names: Disruption Phenotype
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the beta-lactamase family.
messages: Cell membrane
messages: Cells lacking this gene display no visible phenotype during vegetative growth or sporulation.
messages: Expressed during vegetative growth and sporulation.
messages: Up-regulated by sigma-X and sigma-W factors.

224308:BSU 27160
gene id: 224308:BSU_27160
uniprot id: A0A6M3ZEC0
uniprot id: O08336
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Functions as a fatty acid monooxygenase.
messages: Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of a range of medium to long-chain fatty acids, with a preference for long-chain unsaturated and branched-chain fatty acids over saturated fatty acids. Hydroxylation of myristic acid occurs mainly at the omega-2 and omega-3 positions, in approximately equal proportions. Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain.
messages: In the N-terminal section; belongs to the cytochrome P450 family.
messages: Negatively regulated by the transcriptional repressor FatR (PubMed:11574077, PubMed:11734890). Is induced by fatty acids such as oleate, linoleate and phytanate, that bind and displace the FatR repressor (PubMed:11734890). Is also induced by palmitate, likely via another mechanism (PubMed:11574077). Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 32190
gene id: 224308:BSU_32190
uniprot id: A0A6M3ZGJ0
uniprot id: O32116
names: Similarity
messages: Belongs to the UPF0349 family.

224308:BSU 05099
gene id: 224308:BSU_05099
uniprot id: Q9K3A9
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 15210
gene id: 224308:BSU_15210
uniprot id: A0A6M4JG20
uniprot id: P07373
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SEDS family. SpoVE subfamily.
messages: Cell membrane
messages: May play an essential role not only during sporulation, but also during vegetative growth.
messages: Membrane

224308:BSU 33330
gene id: 224308:BSU_33330
uniprot id: A0A6M4JP54
uniprot id: O32204
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2) family.
messages: Catalyzes electroneutral exchange between L-arginine and L-ornithine.
messages: Cell membrane
messages: Has been described as a LysP lysine permease, due in part to the presence of LYS elements in the regulatory region.
messages: Membrane

224308:BSU 39890
gene id: 224308:BSU_39890
uniprot id: P46326
names: Similarity
messages: Belongs to the methyltransferase superfamily.

224308:BSU 23800
gene id: 224308:BSU_23800
uniprot id: A0A6M4JNE1
uniprot id: P54552
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the pyrroline-5-carboxylate reductase family.
messages: Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.
messages: Cytoplasm
messages: The proG proH proI triple mutant is auxotrophic for proline.

224308:BSU 32800
gene id: 224308:BSU_32800
uniprot id: A0A6M3ZGQ1
uniprot id: O32174
names: Cofactor
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the GcvH family.
messages: Binds 1 lipoyl cofactor covalently.
messages: Is also involved in protein lipoylation via its role as an octanoyl/lipoyl carrier protein intermediate.
messages: Strains are lipoate auxotrophs. The requirement for lipoic acid can be bypassed by addition of both acetate and branched-chain fatty acid (BCFA) precursors.
messages: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.
messages: The glycine cleavage system is composed of four proteins: P, T, L and H.

224308:BSU 14700
gene id: 224308:BSU_14700
uniprot id: A0A6M4JLH1
uniprot id: P68736
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M4 family.
messages: Binds 1 zinc ion per subunit.
messages: Binds 4 Ca(2+) ions per subunit.
messages: Extracellular zinc metalloprotease.
messages: Secreted

224308:BSU 22940
gene id: 224308:BSU_22940
uniprot id: P50738
names: Disruption Phenotype
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the protease PrsW family.
messages: Cell membrane
messages: Involved in the degradation of anti-sigma-W factor RsiW. Responsible for Site-1 cleavage of the RsiW anti-sigma factor. This results, after two other proteolytic steps catalyzed by the RasP and ClpXP proteases, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor. Seems to be responsible for sensing antimicrobial peptides that damage the cell membrane and other agents that cause cell envelope stress. Therefore it is a protease governing regulated intramembrane proteolysis and resistance to antimicrobial peptides in B.subtilis.
messages: Loss of expression of the SigW regulon (PubMed:17020587).

224308:BSU 25950
gene id: 224308:BSU_25950
uniprot id: P45938
names: Similarity
messages: To B.subtilis XkdV.

224308:BSU 30240
gene id: 224308:BSU_30240
uniprot id: A0A6M3ZIY3
uniprot id: P53559
names: Function
names: Similarity
names: Subunit
messages: Belongs to the BioW family.
messages: Catalyzes the transformation of pimelate into pimeloyl-CoA with concomitant hydrolysis of ATP to AMP.
messages: Homodimer.

224308:BSU 26240
gene id: 224308:BSU_26240
uniprot id: P45912
names: Similarity
messages: To B.subtilis XtrA.

224308:BSU 02670
gene id: 224308:BSU_02670
uniprot id: A0A6M3Z784
uniprot id: O35018
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the major facilitator superfamily. EmrB family.
messages: Cell membrane
messages: Membrane
messages: Proton-dependent transporter. May mediate the efflux of lincomycin.

224308:BSU 26990
gene id: 224308:BSU_26990
uniprot id: A0A6M4JJP3
uniprot id: O06010
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CotF family.
messages: Spore coat

224308:BSU 37130
gene id: 224308:BSU_37130
uniprot id: P06628
names: Cofactor
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Binds 1 Mg(2+) ion per subunit.
messages: Cytoplasm
messages: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
messages: Phosphorylated by KinA and KinB. Dephosphorylated by RapA and RapB.

224308:BSU 39520
gene id: 224308:BSU_39520
uniprot id: A0A6M3ZLS6
uniprot id: P54950
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the NtaA/SnaA/DszA monooxygenase family.
messages: Catalyzes the oxidative cleavage of the C-S bond of N-acetyl-S-(2-succino)cysteine, forming oxaloacetate and N-acetylcysteine (NAC) (PubMed:34510734). Is involved in a S-(2-succino)cysteine (2SC) degradation pathway that allows B.subtilis to grow on 2SC as a sole sulfur source, via its metabolization to cysteine (PubMed:29626092). Shows almost no activity on S-succinylglutathione and 2SC (PubMed:34510734).
messages: Cells lacking this gene almost lose the ability to grow on 2SC as the sulfur source but are still able to grow on sulfate. Moreover, they show a massive accumulation of N-acetyl-S-(2-succino)cysteine when S2C is added to the medium.
messages: Homodimer.
messages: The substrate N-acetyl-S-(2-succino)-L-cysteine arises from the spontaneous Michael addition of cysteine to the citric acid cycle intermediate fumarate.

224308:BSU 36710
gene id: 224308:BSU_36710
uniprot id: A0A6M4JQ08
uniprot id: P39756
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the FdhD family.
messages: Cytoplasm
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Required for formate dehydrogenase (FDH) activity (PubMed:7860592). Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH (By similarity).
messages: Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.

224308:BSU 25220
gene id: 224308:BSU_25220
uniprot id: Q7WY63
names: Developmental Stage
names: Induction
names: Miscellaneous
messages: Entirely encoded within the dnaG gene, on the opposite strand.
messages: Expressed during early sporulation.
messages: Expression is sigma A-dependent.

224308:BSU 00890
gene id: 224308:BSU_00890
uniprot id: A0A6M4JJ92
uniprot id: Q06754
names: Function
names: Induction
names: Similarity
messages: An RNase.
messages: Belongs to the ycf81 family.
messages: In the central section; belongs to the PINc/VapC protein family.
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969).

224308:BSU 34230
gene id: 224308:BSU_34230
uniprot id: A0A6M3ZH60
uniprot id: Q795J3
names: Function
names: Induction
names: Similarity
messages: Belongs to the DegT/DnrJ/EryC1 family.
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Repressed by SinR.

224308:BSU 38100
gene id: 224308:BSU_38100
uniprot id: A0A6M4JQI7
uniprot id: P39606
names: Similarity
messages: To bacterial alkanal monooxygenase alpha and beta chains.

224308:BSU 33150
gene id: 224308:BSU_33150
uniprot id: A0A6M3ZGA3
uniprot id: O34899
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the Cob(I)alamin adenosyltransferase family.
messages: Cytoplasm
messages: Homotrimer.

224308:BSU 38180
gene id: 224308:BSU_38180
uniprot id: A0A6M3ZJ15
uniprot id: O32282
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0410 family.
messages: Cell membrane
messages: Membrane

224308:BSU 40850
gene id: 224308:BSU_40850
uniprot id: A0A6M3ZJ64
uniprot id: P37515
names: Function
names: Similarity
names: Subunit
messages: Belongs to the transferase hexapeptide repeat family.
messages: Catalyzes the CoA-dependent transfer of an acetyl group to maltose and other sugars. Acetylates glucose exclusively at the C6 position and maltose at the C6 position of the non-reducing end glucosyl moiety. Is able to acetylate maltooligosaccharides.
messages: Homodimer.

224308:BSU 15560
gene id: 224308:BSU_15560
uniprot id: A0A6M4JIG9
uniprot id: P25972
names: Caution
names: Function
names: Similarity
names: Subunit
messages: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.
messages: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
messages: Homodimer (By similarity). Interacts with BrxC (PubMed:33722570).
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 05920
gene id: 224308:BSU_05920
uniprot id: O05516
names: Caution
names: Disruption Phenotype
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the KAE1 / TsaD family. TsaB subfamily.
messages: Cells lacking this gene display a slow growth phenotype. Cell size, shape and nucleoid morphology remain unchanged.
messages: Cytoplasm
messages: Forms a heterodimer with TsaE.
messages: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD.
messages: The four proteins YwlC, TsaD, TsaB and TsaE are necessary and sufficient for tRNA(NNU) t(6)A37 threonylcarbamoyladenosine biosynthesis in vitro in B.subtilis.
messages: The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in B.subtilis and many other species (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA.

224308:BSU 17090
gene id: 224308:BSU_17090
uniprot id: O34769
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the metallo-beta-lactamase superfamily.
messages: Binds 2 Zn(2+) ions per subunit.
messages: Cytoplasm
messages: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.

224308:BSU 15570
gene id: 224308:BSU_15570
uniprot id: A0A6M4JHZ8
uniprot id: P94498
names: Cofactor
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PAPS reductase family. CysH subfamily.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.
messages: Cytoplasm
messages: Up-regulated by sulfur starvation and repressed by cysteine. Also induced by O-acetyl-L-serine (OAS), a direct precursor of cysteine, maybe via inactivation of a putative transcriptional repressor of the cysH operon whose activity is controlled by the intracellular levels of OAS.

224308:BSU 25390
gene id: 224308:BSU_25390
uniprot id: A0A6M3ZED7
uniprot id: P54465
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NfeD family.
messages: Cell membrane
messages: Membrane
messages: No change in localization of FloA or FloT.

224308:BSU 29540
gene id: 224308:BSU_29540
uniprot id: A0A6M3ZF24
uniprot id: O34934
names: Caution
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the NAD kinase family.
messages: Cytoplasm
messages: Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 27030
gene id: 224308:BSU_27030
uniprot id: A0A6M3ZED6
uniprot id: P05656
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the glycosyl hydrolase 32 family.
messages: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose.
messages: Induced by low concentrations of fructose, but not by sucrose. Repressed by glucose or high concentrations of fructose.
messages: Is completely inhibited by Ag(+) and Hg(2+) ions.
messages: Levanase cannot be detected in the wild-type B.subtilis but is mostly secreted into the culture medium by SacL mutants, especially at the end of the exponential growth phase.
messages: Secreted

224308:BSU 34265
gene id: 224308:BSU_34265
uniprot id: A0A6M3ZGU6
uniprot id: P71060
names: Function
names: Induction
names: Subcellular Location Annotation
messages: Cell membrane
messages: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles.
messages: Membrane
messages: Repressed by SinR.

224308:BSU 14650
gene id: 224308:BSU_14650
uniprot id: A0A6M4JIA3
uniprot id: Q45498
names: Similarity
messages: Belongs to the UPF0637 family.

224308:BSU 02770
gene id: 224308:BSU_02770
uniprot id: P46905
names: Similarity
messages: Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

224308:BSU 29770
gene id: 224308:BSU_29770
uniprot id: P40780
names: Similarity
messages: To C.plantagineum desiccation-related protein clone PCC3-06.

224308:BSU 05980
gene id: 224308:BSU_05980
uniprot id: A0A6M3Z8F9
uniprot id: O05522
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: B.subtilis possesses two minimal, substrate-specific, Tat translocases: TatAd-TatCd and TatAy-TatCy, each one composed of a TatA and a TatC protein. TatA is bifunctional and performs the function of both the TatA and TatB proteins of Gram-negative organisms.
messages: Belongs to the TatA/E family.
messages: Cell membrane
messages: Forms a complex with TatC.
messages: Forms a complex with TatCy. Two types of complexes exist: one composed of TatAy and TatCy, and another composed only of TatAy. Cytosolic TatA forms large complexes or aggregates.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. Required for YwbN secretion.
messages: cytosol

224308:BSU 05500
gene id: 224308:BSU_05500
uniprot id: A0A6M3Z7V2
uniprot id: P96694
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the DoxX family.
messages: Cell membrane
messages: Membrane
messages: Putative oxidoreductase that may contribute to the degradation of aromatic compounds.
messages: Repressed by MhqR. Strongly induced by stress due to exposure to 2-methylhydroquinone (2-MHQ) and less strongly induced after diamide or catechol stress. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

224308:BSU 12510
gene id: 224308:BSU_12510
uniprot id: P23789
names: Function
names: Miscellaneous
messages: Repressor of PBSX. Binds to four sites close to its own gene. Necessary for the maintenance of the lysogenic state.
messages: The defective prophage of Bacillus subtilis 168, PBSX, is a chromosomally based element which encodes a non-infectious phage-like particle with bactericidal activity. PBSX is induced by agents which elicit the SOS response (PubMed:2125016).

224308:BSU 08910
gene id: 224308:BSU_08910
uniprot id: A0A6M3Z8U0
uniprot id: P97030
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the QueG family.
messages: Binds 2 [4Fe-4S] clusters per monomer.
messages: Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
messages: Cytoplasm
messages: Monomer.

224308:BSU 15950
gene id: 224308:BSU_15950
uniprot id: A0A6M4JI60
uniprot id: P51835
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the GTP-binding SRP family. FtsY subfamily.
messages: Cell membrane
messages: Cytoplasm
messages: Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).
messages: Membrane
messages: Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.

224308:BSU 02650
gene id: 224308:BSU_02650
uniprot id: A0A6M4JI67
uniprot id: P28618
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the peptidase C15 family.
messages: Cytoplasm
messages: Homotetramer.
messages: Removes 5-oxoproline from various penultimate amino acid residues except L-proline.

224308:BSU 27880
gene id: 224308:BSU_27880
uniprot id: A0A6M3ZEQ2
uniprot id: P38033
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
messages: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
messages: Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity). Seems to be required for NAD biosynthesis (PubMed:8444804).
messages: Cells lacking this gene require nicotinic acid for growth.
messages: Repressed in the presence of nicotinic acid.

224308:BSU 15250
gene id: 224308:BSU_15250
uniprot id: A0A6M3ZAF2
uniprot id: C0SPA3
names: Function
names: Induction
names: Similarity
messages: Belongs to the UPF0749 family.
messages: By vancomycin.
messages: May be involved in cell division and sporulation.

224308:BSU 09720
gene id: 224308:BSU_09720
uniprot id: A0A6M4JGJ5
uniprot id: O07549
names: Activity Regulation
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Heterodimer composed of YheH and YheI.
messages: Induced by several classes of structurally unrelated antibiotics, such as erythromycin, fusidic acid or linezolid.
messages: Inhibited by ortho-vanadate.
messages: Involved in the transport of four structurally unrelated drugs, including doxorubicin and mitoxantrone. Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation.
messages: Membrane
messages: Overexpression reduces the sporulation efficiency possibly by modulating the function of KinA.

224308:BSU 34180
gene id: 224308:BSU_34180
uniprot id: O07007
names: Disruption Phenotype
names: Function
messages: Cells lacking this gene show a derepression of lutABC expression. They are also defective in bacilysin production.
messages: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to LutR, leads to derepression of the operon. Also appears to be essential for bacilysin biosynthesis.

224308:BSU 00460
gene id: 224308:BSU_00460
uniprot id: A0A6M4JR72
uniprot id: P37550
names: Function
names: Similarity
messages: Belongs to the GHMP kinase family. IspE subfamily.
messages: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.

224308:BSU 39490
gene id: 224308:BSU_39490
uniprot id: A0A6M3ZHY3
uniprot id: P54953
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the binding-protein-dependent transport system permease family.
messages: Cell membrane
messages: Membrane
messages: More strongly expressed in the presence of methionine than in the presence of sulfate.
messages: Probably part of the ABC transporter complex YxeMNO that could be involved in amino-acid import. May transport S-methylcysteine. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (YxeO), two transmembrane proteins (YxeN) and a solute-binding protein (YxeM).

224308:BSU 15670
gene id: 224308:BSU_15670
uniprot id: A0A6M4JI06
uniprot id: Q7WY72
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Similarity
messages: Belongs to the RemA family.
messages: Expressed in the forespore during sporulation.
messages: Mutation impairs pellicle formation, complex colony architecture and motility inhibition in a sinR mutant background (PubMed:19363116). Mutant is defective in biofilm formation and also shows a delay in spore formation during biofilm formation (PubMed:23396918).
messages: Regulates the biosynthesis of the extracellular matrix and the biofilm formation (PubMed:19363116, PubMed:23646920). Activates the transcription of the matrix biosynthesis operons eps and tapA-sipW-tasA. Also activates expression of the opuA operon, involved in osmoprotection. Acts by binding specifically to the promoter region of the target genes (PubMed:23646920). Acts in parallel to the pathway that governs SinR derepression (PubMed:19363116, PubMed:23646920). Required for endosporulation (PubMed:23396918).

224308:BSU 21870
gene id: 224308:BSU_21870
uniprot id: A0A6M3ZDB3
uniprot id: P51785
names: Caution
names: Cofactor
names: Function
names: Similarity
names: Subunit
messages: Belongs to the IlvD/Edd family.
messages: Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.
messages: Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.
messages: Homodimer.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.

224308:BSU 10930
gene id: 224308:BSU_10930
uniprot id: A0A6M3Z9U9
uniprot id: O06737
names: Cofactor
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PAPS reductase family. CysH subfamily.
messages: Binds 1 [4Fe-4S] cluster per subunit.
messages: Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.
messages: Cytoplasm

224308:BSU 21830
gene id: 224308:BSU_21830
uniprot id: P54173
names: Similarity
messages: To B.subtilis YutG.

224308:BSU 36240
gene id: 224308:BSU_36240
uniprot id: P96717
names: Activity Regulation
names: Function
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily. CpsB/CapC family.
messages: Dephosphorylates the phosphotyrosine-containing proteins YwqD, YwqF and Ssb.
messages: Inhibited by vanadate and sodium pyrophosphate. Not inhibited by sodium fluoride.

224308:BSU 18450
gene id: 224308:BSU_18450
uniprot id: A0A6M3ZBC2
uniprot id: P39812
names: Cofactor
names: Induction
names: Similarity
names: Subunit
messages: Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.
messages: Belongs to the glutamate synthase family.
messages: Binds 1 [3Fe-4S] cluster.
messages: The gltAB operon is positively regulated by GltC and negatively regulated by TnrA under nitrogen-limited conditions.

224308:BSU 38400
gene id: 224308:BSU_38400
uniprot id: A0A6M3ZIF9
uniprot id: P16396
names: Activity Regulation
names: Disruption Phenotype
names: Domain
names: Function
names: Induction
names: PTM
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S8 family.
messages: Contains two domains, a large N-terminal domain encoding a serine protease homologous to subtilisin and a smaller C-terminal domain that possesses unusual sequence features (PubMed:2116590, PubMed:3142851, PubMed:16553828). The C-terminal third of the protein is not required for protease activity but is required for swarming (PubMed:2116590, PubMed:3142851, PubMed:16553828).
messages: Disruption of the gene abolishes swarming (PubMed:11751842, PubMed:19416356). Mutant lacking this gene does not show significant decrease in CSF production, but the triple deletion mutant aprE-epr-vpr is defective in the cleavage event to release mature CSF (PubMed:17666034).
messages: Expression is sigma D-dependent (PubMed:11751842). Expression is positively regulated by DegU, and very low levels of DegU-P are required for epr maximal expression (PubMed:19416356). Negatively regulated jointly by ScoC (Hpr) and SinR, which bind to their respective target sites 62 bp apart (PubMed:16923912). Spo0A positively regulates epr expression by negating the repressive effect of corepressors, SinR and ScoC (Hpr) (PubMed:23660663). Spo0A binds to the upstream region of epr promoter and in turn occludes the binding site of one of the corepressor, SinR (PubMed:23660663).
messages: May undergo two steps of processing in its passage through the cell membrane: removal of the N-terminal signal sequence and cleavage of the C-terminal domain (PubMed:16553828). Several active forms of Epr with molecular masses between 40 and 34 kDa were found in the medium of B.subtilis cultures (PubMed:2116590). The size variation of the active forms expressed by the complete epr gene appears to be the result of partial removal of the C-terminus either by processing or degradation (PubMed:2116590).
messages: Requires Ca(2+) for stability (PubMed:2116590). Activity is inhibited by phenylmethylsulfonyl fluoride (PMSF) and EDTA (PubMed:2116590, PubMed:3142851).
messages: Secreted
messages: Serine protease (PubMed:3142851, PubMed:2116590, PubMed:17666034). Involved in the production of the competence and sporulation stimulating factor CSF (PubMed:17666034). In addition, is essential for swarming motility (PubMed:11751842, PubMed:16553828, PubMed:19416356). Plays a key role in DegU-mediated swarming motility (PubMed:19416356). The protease activity is dispensable for swarming (PubMed:16553828). Not essential for growth or sporulation (PubMed:3142851).
messages: cell wall

224308:BSU 39950
gene id: 224308:BSU_39950
uniprot id: P42109
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 40080
gene id: 224308:BSU_40080
uniprot id: A0A6M4JR43
uniprot id: P12013
names: Disruption Phenotype
names: Function
names: Similarity
names: Subunit
messages: Belongs to the 6-phosphogluconate dehydrogenase family.
messages: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NAD to NADH. Does not contribute to oxidative pentose phosphate (PP) pathway fluxes during growth on glucose. The functional role of GntZ remains obscure.
messages: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
messages: Cells lacking this gene exhibit no detectable phenotype on glucose as the sole carbon source, and they grow normally on gluconate.
messages: Homodimer.

224308:BSU 10390
gene id: 224308:BSU_10390
uniprot id: O07622
names: Cofactor
names: Similarity
messages: Belongs to the Rieske iron-sulfur protein family.
messages: Binds 1 [2Fe-2S] cluster per subunit.

224308:BSU 29090
gene id: 224308:BSU_29090
uniprot id: A0A6M4JKZ0
uniprot id: O34996
names: Function
names: Similarity
names: Subunit
messages: Belongs to the DNA polymerase type-A family.
messages: In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.
messages: In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.
messages: Single-chain monomer with multiple functions.

224308:BSU 01700
gene id: 224308:BSU_01700
uniprot id: A0A6M3Z6V2
uniprot id: Q45582
names: Function
names: Miscellaneous
names: Similarity
names: Subunit
messages: A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
messages: Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.
messages: Homodimer.
messages: Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate.

224308:BSU 03710
gene id: 224308:BSU_03710
uniprot id: A0A6M4JFF9
uniprot id: P49941
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the GerABKC lipoprotein family.
messages: Cell membrane
messages: Involved in the germination response to the combination of glucose, fructose, L-asparagine, and KCl.

224308:BSU 13260
gene id: 224308:BSU_13260
uniprot id: A0A6M4JFG2
uniprot id: O34638
names: Function
names: Subcellular Location Annotation
messages: Cell membrane
messages: Membrane
messages: Probable member of the two-component regulatory system YkoH/YkoG. Potentially phosphorylates YkoG.

224308:BSU 13910
gene id: 224308:BSU_13910
uniprot id: A0A6M3ZFF8
uniprot id: P08838
names: Domain
names: Function
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the PEP-utilizing enzyme family.
messages: Cytoplasm
messages: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
messages: Homodimer (By similarity). Interacts with FloT (PubMed:23651456).
messages: Membrane raft
messages: The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.
messages: The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain.

224308:BSU 03320
gene id: 224308:BSU_03320
uniprot id: P42433
names: Function
names: Induction
messages: Positively regulated by TnrA under nitrogen-limited conditions.
messages: Required for nitrate assimilation.

224308:BSU 23310
gene id: 224308:BSU_23310
uniprot id: A0A6M4JNL5
uniprot id: P28628
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.
messages: Belongs to the peptidase S26 family.
messages: Cell membrane
messages: Expressed at the postexponential growth phase; regulated by the DegS-DegU system.
messages: Membrane
messages: Not essential for cell viability, but required for efficient secretion of many proteins.

224308:BSU 14570
gene id: 224308:BSU_14570
uniprot id: P21884
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 35680
gene id: 224308:BSU_35680
uniprot id: P46918
names: Function
names: Similarity
messages: Belongs to the glycosyltransferase 2 family.
messages: Involved in the biosynthesis of galactosamine-containing minor teichoic acid, a non-essential cell wall polymer in B.subtilis 168.

224308:BSU 16600
gene id: 224308:BSU_16600
uniprot id: A0A6M3ZEF9
uniprot id: P32727
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the NusA family.
messages: Cytoplasm
messages: Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.
messages: Participates in both transcription termination and antitermination.

224308:BSU 38499
gene id: 224308:BSU_38499
uniprot id: C0H3T3
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 02100
gene id: 224308:BSU_02100
uniprot id: O31440
names: Function
names: Similarity
messages: Belongs to the cytochrome P450 family.
messages: Catalyzes the alpha- and beta-hydroxylation of myristic acid in the presence of hydrogen peroxide.

224308:BSU 09210
gene id: 224308:BSU_09210
uniprot id: A0A6M4JGC1
uniprot id: P54604
names: Function
names: Similarity
messages: Belongs to the pseudouridine synthase RluA family.
messages: Responsible for synthesis of pseudouridine from uracil.

224308:BSU 05710
gene id: 224308:BSU_05710
uniprot id: O05495
names: Developmental Stage
names: Disruption Phenotype
names: Domain
names: Miscellaneous
names: Similarity
names: Subcellular Location Annotation
messages: According to PubMed:11011148, cells show no effect vegetative growth, spore resistance to heat, chloroform and lysozyme, or spore germination in the presence of L-alanine. According to PubMed:12177332, cells have no detectable alteration in either dormant or germinating spore peptidoglycan, and germinate normally.
messages: Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
messages: Cells producing excessive ydhD do not show impaired spore resistance, but their germination properties change: spores show reduced response to L-alanine and some of them germinate even without germinants.
messages: Expressed in the mother cell compartment from T2 of sporulation.
messages: LysM domains are thought to be involved in peptidoglycan binding.
messages: Spore wall
messages: Transcription of ydhD is dependent on SigE.

224308:BSU 37680
gene id: 224308:BSU_37680
uniprot id: P39644
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Along with the bacABCDEF operon, BacG is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:20052993). BacG catalyzes the stereoselective reduction of exocyclic-delta(3),delta(5)-dihydro-hydroxyphenylpyruvate (ex-H2HPP), adding a pro-S hydride equivalent to C4 position to yield tetrahydro-hydroxyphenylpyruvate (H4HPP) (PubMed:22765234, PubMed:23519407). Although the 3Z,7R-ex-H2HPP isomer is kinetically disfavored by BacB and produced in a smaller quantity than 3E,7R-ex-H2HPP, it is the preferred substrate for the conjugate reduction reaction of BacG (PubMed:22765234, PubMed:23519407).
messages: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
messages: Cytoplasm
messages: Homodimer.
messages: The compound guanosine 5'-diphosphate 3'-diphosphate (ppGpp) is essential for the transcription of the bacABCDEF operon and BacG, and GTP regulates the transcription of both this operon and ywfH via the CodY-mediated regulation system.

224308:BSU 14320
gene id: 224308:BSU_14320
uniprot id: A0A6M3ZAU0
uniprot id: O31707
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the ABC transporter superfamily.
messages: Cell membrane
messages: Membrane

224308:BSU 13230
gene id: 224308:BSU_13230
uniprot id: O34738
names: Function
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Part of the ABC transporter complex YkoCDEF that could transport hydroxymethylpyrimidine (HMP) and/or thiamine. Could also transport other HMP-containing products. Probably responsible for the translocation of the substrate across the membrane (Probable).
messages: The complex is composed of two ATP-binding proteins (YkoD), two transmembrane proteins (YkoC and YkoE) and a solute-binding protein (YkoF).

224308:BSU 20999
gene id: 224308:BSU_20999
uniprot id: C0H439
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 14490
gene id: 224308:BSU_14490
uniprot id: P39764
names: Disruption Phenotype
names: Function
names: Induction
names: Subcellular Location Annotation
names: Subunit
messages: Cell membrane
messages: Induced at the onset of sporulation, shuts off at T3. May be under the control of Spo0A.
messages: Membrane raft
messages: No observable effect on sporulation (PubMed:8002615). Forms a weak or no biofilm, wild-type sporulation (PubMed:22882210, PubMed:26297017).
messages: Oligomerizes, probably forms homodimers; oligomerization is assisted by FloT. Interacts with FloT (PubMed:26297017). Another study shows only rare colocalization with FloT or FloA membrane assemblies. KinC membrane assemblies are more mobile than FloT membrane assemblies (PubMed:27362352).
messages: Phosphorylates the sporulation-regulatory protein Spo0A a transcription factor that also controls biofilm formation (Probable). Requires FloT and FloA for localization to DRMs and for activity (PubMed:20713508).

224308:BSU 21010
gene id: 224308:BSU_21010
uniprot id: O31942
names: Subcellular Location Annotation
messages: Cell membrane

224308:BSU 25260
gene id: 224308:BSU_25260
uniprot id: A0A6M4JNG5
uniprot id: P54381
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the class-II aminoacyl-tRNA synthetase family.
messages: Cytoplasm
messages: Tetramer of two alpha and two beta subunits.

224308:BSU 34060
gene id: 224308:BSU_34060
uniprot id: A0A6M4JPC7
uniprot id: O07019
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Member of the two-component regulatory system YvfT/YvfU.
messages: Phosphorylated by YvfT.

224308:BSU 19330
gene id: 224308:BSU_19330
uniprot id: A0A6M3ZC91
uniprot id: O35023
names: Cofactor
names: Function
names: Similarity
messages: Belongs to the iron/manganese superoxide dismutase family.
messages: Binds 1 Fe cation per subunit.
messages: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

224308:BSU 29300
gene id: 224308:BSU_29300
uniprot id: P94465
names: Disruption Phenotype
names: Function
names: Induction
names: Miscellaneous
names: Similarity
messages: Belongs to the RibR family.
messages: May be directly involved in the regulation of the rib genes. C-terminal part of RibR specifically binds to RFN of the rib leader of the riboflavin biosynthetic operon. The RFN element is a sequence within the rib-leader mRNA reported to serve as a receptor for an FMN-dependent riboswitch. Possibly, RibR produces the comodulator FMN through its own N-terminal flavokinase activity. FMN-activated RibR may stabilize the anti-anti terminator structure of RFN mRNA, causing transcription termination of the rib genes in trans.
messages: Mutants lacking this gene are deregulated with respect to flavin (riboflavin, FMN and FAD) biosynthesis.
messages: Overexpression of RibR suppresses the riboflavin-overproducing ribC-defective phenotype, which is the major riboflavin kinase responsible of FMN biosynthesis by riboflavin phosphorylation (PubMed:17590224, PubMed:15668000).
messages: Repressed by the HTH-type transcriptional regulator CymR.

224308:BSU 32730
gene id: 224308:BSU_32730
uniprot id: A0A6M3ZFV3
uniprot id: O32167
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the NlpA lipoprotein family.
messages: Belongs to the nlpA lipoprotein family.
messages: Cell membrane
messages: Membrane
messages: Part of the ABC transporter complex MetNPQ involved in methionine import. Binds the methionine and transfers it to the membrane-bound permease. It has also been shown to be involved in methionine sulfoxide transport (Probable).
messages: Repressed by methionine via the S-box system.
messages: The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetP) and a solute-binding protein (metQ).

224308:BSU 35480
gene id: 224308:BSU_35480
uniprot id: P32436
names: Function
names: Subunit
messages: Binds long-chain fatty acids, such as palmitate, and may play a role in lipid transport or fatty acid metabolism.
messages: Monomer.

224308:BSU 17710
gene id: 224308:BSU_17710
uniprot id: A0A6M4JH30
uniprot id: O31804
names: Function
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the TatA/E family.
messages: Cell membrane
messages: Forms a complex with TatC.
messages: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.

224308:BSU 37530
gene id: 224308:BSU_37530
uniprot id: A0A6M4JQC8
uniprot id: P70995
names: Cofactor
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase M50B family.
messages: Binds 1 zinc ion per subunit.
messages: Cell membrane
messages: Membrane

224308:BSU 18180
gene id: 224308:BSU_18180
uniprot id: A0A6M4JJN0
uniprot id: O31822
names: Function
names: Induction
names: Miscellaneous
names: Similarity
names: Subunit
messages: Belongs to the UDPGP type 2 family.
messages: Catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP (PubMed:33556370). This is an intermediate step in the biosynthesis of diglucosyl-diacylglycerol (Glc2-DAG), i.e. the predominant glycolipid found in B.subtilis membrane, which is also used as a membrane anchor for lipoteichoic acid (LTA) (PubMed:33556370). YngB contributes to wall teichoic acid (WTA) glucosylation and glycolipid formation under anaerobic fermentative growth conditions (PubMed:33556370). Might also enter other glycosylation pathways, leading to the decorating of other cell envelope components with glucose residues under anaerobic or other growth conditions (PubMed:33556370).
messages: Expressed under anaerobic conditions.
messages: GtaB is the main UGPase enzyme producing UDP-glucose under both aerobic and anaerobic fermentative growth conditions (PubMed:33556370). YngB augments UDP-glucose production under anaerobic growth conditions (PubMed:33556370).
messages: Homodimer.

224308:BSU 10750
gene id: 224308:BSU_10750
uniprot id: O06724
names: Induction
names: Similarity
messages: Belongs to the FAH family.
messages: In response to ammonium, tryptophan, glucose, and phosphate starvation.

224308:BSU 35280
gene id: 224308:BSU_35280
uniprot id: A0A6M4JPN1
uniprot id: O34792
names: Disruption Phenotype
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the UPF0750 family.
messages: Cell membrane
messages: Cells lacking this gene show no apparent growth, sporulation, or germination defect. Also shows no defect in the synthesis of the membrane-bound CccB cytochrome or any other cytochrome with covalently bound heme.
messages: Membrane

224308:BSU 32630
gene id: 224308:BSU_32630
uniprot id: O32159
names: Similarity
messages: Belongs to the DadA oxidoreductase family.

224308:BSU 09850
gene id: 224308:BSU_09850
uniprot id: O07536
names: Activity Regulation
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.
messages: Cell membrane
messages: Deletion mutant is more sensitive to methylglyoxal than wild-type.
messages: Part of the khtSTU operon. Induced by salt stress at alkaline pH.
messages: Potassium antiport activity requires the presence of KhtT. Activity is also modulated by KhtS. Has higher activity at alkaline pH.
messages: Potassium/proton antiporter that mediates the efflux of potassium ions from the cell (PubMed:17679694). Can also mediate rubidium/proton antiport, but has no permeability for sodium or lithium ions. In the absence of KhtT, does not have antiport activity, but can catalyze potassium efflux (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis, via activation by S-lactoyl-BSH of the antiporter activity, leading to cytoplasmic acidification and methylglyoxal resistance (PubMed:24330391).
messages: The transporter is composed of the integral membrane protein KhtU and the regulatory protein KhtT.

224308:BSU 02450
gene id: 224308:BSU_02450
uniprot id: P40759
names: Function
names: Miscellaneous
names: PTM
names: Subcellular Location Annotation
messages: Cytoplasm
messages: Disruption of glnL by Tn917 insertion leads to protonophore-resistance.
messages: Member of the two-component regulatory system GlnL/GlnK that positively regulates the expression of the glsA-glnT operon in response to glutamine. GlnL binds the promoter region of glsA-glnT in vitro.
messages: Phosphorylated by GlnK.

224308:BSU 23480
gene id: 224308:BSU_23480
uniprot id: A0A6M3ZGW3
uniprot id: P38422
names: Developmental Stage
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the peptidase S11 family.
messages: Expressed in the forespore.
messages: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
messages: Secreted

224308:BSU 26410
gene id: 224308:BSU_26410
uniprot id: A0A6M3ZKY6
uniprot id: P54445
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the PsiE family.
messages: Cell inner membrane
messages: Cell membrane
messages: Membrane

224308:BSU 08290
gene id: 224308:BSU_08290
uniprot id: P94438
names: Disruption Phenotype
names: Function
names: PTM
names: Subcellular Location Annotation
messages: Autophosphorylated.
messages: Cell membrane
messages: Deletion mutant does not show any observable phenotype.
messages: Required for resistance to linearmycins, a family of antibiotic-specialized metabolites produced by some streptomycetes (PubMed:26647299, PubMed:28461449). Member of the two-component regulatory system LnrJ/LnrK, which induces expression of the LnrLMN ABC transporter in response to linearmycins and other polyenes (PubMed:11717295, PubMed:26647299, PubMed:28461449). Acts as a specific sensor for linearmycin, either directly through binding or indirectly through membrane perturbation. Probably activates LnrK by phosphorylation (PubMed:28461449). May also promote biofilm formation (PubMed:28461449).

224308:BSU 36620
gene id: 224308:BSU_36620
uniprot id: P71037
names: Caution
messages: The N-terminus share sequence similarity with the dihydrodipicolinate reductase family. It however lacks the conserved C-terminal part, suggesting it has no dihydrodipicolinate reductase activity.

224308:BSU 26470
gene id: 224308:BSU_26470
uniprot id: P54439
names: Similarity
messages: Belongs to the NAD(P)H dehydrogenase (quinone) family.

224308:BSU 32120
gene id: 224308:BSU_32120
uniprot id: O32111
names: Subcellular Location Annotation
messages: Membrane

224308:BSU 15730
gene id: 224308:BSU_15730
uniprot id: A0A6M4JG85
uniprot id: P94463
names: Function
names: Similarity
messages: Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.
messages: Belongs to the Fmt family.

224308:BSU 08830
gene id: 224308:BSU_08830
uniprot id: P97027
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the ABC transporter superfamily. Aliphatic sulfonates importer (TC 3.A.1.17.2) family.
messages: Cell membrane
messages: Part of the ABC transporter complex SsuABC involved in aliphatic sulfonates import. Responsible for energy coupling to the transport system (Probable). Is also involved in taurine transport.
messages: Repressed by sulfate and cysteine.
messages: The complex is composed of two ATP-binding proteins (SsuB), two transmembrane proteins (SsuC) and a solute-binding protein (SsuA).

224308:BSU 27090
gene id: 224308:BSU_27090
uniprot id: A0A6M3ZHR5
uniprot id: O06005
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the amino acid-polyamine-organocation (APC) superfamily.
messages: Cell membrane
messages: Membrane
messages: Probable amino-acid or metabolite transport protein.

224308:BSU 22750
gene id: 224308:BSU_22750
uniprot id: A3F3D8
uniprot id: P31113
names: Caution
names: Function
names: Similarity
messages: Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2).

224308:BSU 06430
gene id: 224308:BSU_06430
uniprot id: A0A6M3Z831
uniprot id: P12045
names: Function
names: Similarity
names: Subunit
messages: Belongs to the PurK/PurT family.
messages: Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).
messages: Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).
messages: Homodimer.

224308:BSU 16940
gene id: 224308:BSU_16940
uniprot id: A0A6M4JN18
uniprot id: P16971
names: Developmental Stage
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
names: Subunit
messages: Belongs to the RecA family.
messages: By competence, expression activated by ComK (PubMed:11948146). By DNA damage.
messages: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.
messages: Cells lacking this gene are extremely sensitive to DNA-damaging agents. They show 4-fold impaired growth in their absence.
messages: Cytoplasm
messages: Induced during competence (PubMed:11948146).
messages: Monomer; forms higher-order oligomers (PubMed:16061691). Interacts with RecU (PubMed:18684995). Interacts with DprA (smf) (PubMed:17803906).
messages: Multifunctional protein involved in homologous recombination, DNA repair and competence (PubMed:16061691, PubMed:16385024, PubMed:17803906, PubMed:18684995, PubMed:25138221). Can catalyze the hydrolysis of (d)ATP in the presence of single-stranded DNA; prefers dATP at least in vitro, catalyzes the dATP-dependent uptake of single-stranded DNA by duplex DNA, and the dATP-dependent hybridization of homologous single-stranded DNAs (strand exchange) (PubMed:25138221). RecA-ATP cannot catalyze homologous DNA strand exchange; SsbA and DprA activate strand exchange by RecA-ATP (PubMed:25138221). It interacts with LexA causing its activation and leading to its autocatalytic cleavage. Hydrolysis of ATP in the presence of single-stranded DNA is partially inhibited by RecU (PubMed:18684995). Required for DNA transformation; protects transforming DNA from degradation, possibly in combination with DprA (PubMed:17803906).
messages: Recruited to repair centers (RCs), foci that are the site of double-stranded DNA break(s), after RecN (PubMed:16061691). Concomitant with the appearance of RecO at the RCs, RecA forms threads that extend from RCs toward the opposite cell half, possibly searching for sequence homology along the sister chromosome. The threads disappear after about 2 hours (PubMed:16061691). Thread formation is absolutely dependent on RecJ or AadAB (PubMed:16385024).
messages: nucleoid

224308:BSU 27400
gene id: 224308:BSU_27400
uniprot id: A0A6M3ZEV5
uniprot id: Q7WY61
names: Similarity
messages: Belongs to the UPF0297 family.

224308:BSU 32469
gene id: 224308:BSU_32469
uniprot id: A0A6M3ZG36
uniprot id: C0H3Q7
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the SscA family.
messages: Membrane

224308:BSU 38720
gene id: 224308:BSU_38720
uniprot id: A0A6M3ZHH4
uniprot id: P94368
names: Function
names: Similarity
names: Subunit
messages: Belongs to the NnrD/CARKD family.
messages: Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
messages: Homotetramer.

224308:BSU 06560
gene id: 224308:BSU_06560
uniprot id: A0A6M3Z8R4
uniprot id: O34909
names: Similarity
messages: Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.

224308:BSU 28900
gene id: 224308:BSU_28900
uniprot id: A0A6M4JM20
uniprot id: P94516
names: Function
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the CidB/LrgB family. LrgB subfamily.
messages: Cell membrane
messages: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgA, with the holin-like protein CidA. The LrgAB and CidA proteins may affect the proton motive force of the membrane. May be involved in programmed cell death (PCD), possibly triggering PCD in response to antibiotics and environmental stresses.
messages: Membrane

224308:BSU 04230
gene id: 224308:BSU_04230
uniprot id: A0A6M4JGG7
uniprot id: P96581
names: Caution
names: Disruption Phenotype
names: Function
names: Induction
names: Similarity
names: Subcellular Location Annotation
messages: Belongs to the Amj family.
messages: Cell membrane
messages: Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.
messages: Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane. May serve as a defense mechanism against naturally occurring MurJ antagonists.
messages: Lacks conserved residue(s) required for the propagation of feature annotation.
messages: Mutants lacking both murJ and amj have a lethal defect in cell wall synthesis.
messages: Transcribed under partial control of SigM ECF sigma factor (PubMed:17434969, PubMed:25918422). Expression is up-regulated in the absence of MurJ (PubMed:25918422).