http://togogenome.org/gene/386911:G6N37_RS04565 ^@ http://purl.uniprot.org/uniprot/A0A7I7NX48 ^@ Similarity ^@ Belongs to the thioredoxin family. http://togogenome.org/gene/386911:G6N37_RS24105 ^@ http://purl.uniprot.org/uniprot/A0A7I7P8L9 ^@ Function|||Similarity ^@ Belongs to the universal ribosomal protein uS19 family.|||Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. http://togogenome.org/gene/386911:G6N37_RS14970 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0T3 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/386911:G6N37_RS02165 ^@ http://purl.uniprot.org/uniprot/A0A7I7NTS0 ^@ Similarity ^@ Belongs to the P(II) protein family. http://togogenome.org/gene/386911:G6N37_RS24590 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6L2 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL13 family.|||Part of the 50S ribosomal subunit.|||This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. http://togogenome.org/gene/386911:G6N37_RS00830 ^@ http://purl.uniprot.org/uniprot/A0A7I7NSU7 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the complex I subunit 4L family.|||Cell membrane|||Membrane|||NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.|||NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. http://togogenome.org/gene/386911:G6N37_RS14975 ^@ http://purl.uniprot.org/uniprot/A0A7I7P360 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/386911:G6N37_RS24240 ^@ http://purl.uniprot.org/uniprot/A0A7I7P669 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL15 family.|||Binds to the 23S rRNA.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/386911:G6N37_RS24210 ^@ http://purl.uniprot.org/uniprot/A0A7I7P711 ^@ Cofactor|||Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.|||Binds 1 zinc ion per subunit.|||Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.|||Part of the 30S ribosomal subunit. Contacts proteins S3 and S10. http://togogenome.org/gene/386911:G6N37_RS08610 ^@ http://purl.uniprot.org/uniprot/A0A7I7P096 ^@ Caution|||Function|||Similarity|||Subunit ^@ Belongs to the RNA polymerase-binding protein RbpA family.|||Binds to RNA polymerase (RNAP), stimulating transcription from principal, but not alternative sigma factor promoters.|||Forms a complex with the RNAP catalytic core and with free principal sigma factors.|||Lacks conserved residue(s) required for the propagation of feature annotation. http://togogenome.org/gene/386911:G6N37_RS24095 ^@ http://purl.uniprot.org/uniprot/A0A7I7P638 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL23 family.|||One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.|||Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome. http://togogenome.org/gene/386911:G6N37_RS13465 ^@ http://purl.uniprot.org/uniprot/A0A7I7P154 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the prokaryotic pantothenate kinase family.|||Cytoplasm http://togogenome.org/gene/386911:G6N37_RS00110 ^@ http://purl.uniprot.org/uniprot/A0A7I7NSX9 ^@ Cofactor|||Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ Acts as a transcriptional regulator. Probably redox-responsive. The apo- but not holo-form probably binds DNA.|||Belongs to the WhiB family.|||Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.|||Cytoplasm|||The Fe-S cluster can be nitrosylated by nitric oxide (NO).|||Upon Fe-S cluster removal intramolecular disulfide bonds are formed. http://togogenome.org/gene/386911:G6N37_RS23800 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6K9 ^@ Similarity ^@ Belongs to the bacterial ribosomal protein bL33 family. http://togogenome.org/gene/386911:G6N37_RS24335 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6G3 ^@ Caution|||Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the SecY/SEC61-alpha family.|||Cell membrane|||Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.|||Lacks conserved residue(s) required for the propagation of feature annotation.|||Membrane|||The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. http://togogenome.org/gene/386911:G6N37_RS21295 ^@ http://purl.uniprot.org/uniprot/A0A7I7P4I0 ^@ Similarity ^@ Belongs to the CsoR family. http://togogenome.org/gene/386911:G6N37_RS06935 ^@ http://purl.uniprot.org/uniprot/A0A7I7NZB0 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the sbp family.|||Cell membrane http://togogenome.org/gene/386911:G6N37_RS24200 ^@ http://purl.uniprot.org/uniprot/A0A7I7P8M3 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL24 family.|||One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.|||One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/386911:G6N37_RS24215 ^@ http://purl.uniprot.org/uniprot/A0A7I7P655 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS8 family.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.|||Part of the 30S ribosomal subunit. Contacts proteins S5 and S12. http://togogenome.org/gene/386911:G6N37_RS14250 ^@ http://purl.uniprot.org/uniprot/A0A7I7P118 ^@ Similarity ^@ Belongs to the bacterial ribosomal protein bL32 family. http://togogenome.org/gene/386911:G6N37_RS03030 ^@ http://purl.uniprot.org/uniprot/A0A7I7NTW4 ^@ Cofactor|||Function|||Similarity ^@ Belongs to the NrdR family.|||Binds 1 zinc ion.|||Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes. http://togogenome.org/gene/386911:G6N37_RS13725 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1A6 ^@ Similarity ^@ Belongs to the WXG100 family. http://togogenome.org/gene/386911:G6N37_RS24520 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6B8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS11 family.|||Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.|||Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3. http://togogenome.org/gene/386911:G6N37_RS14900 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0R5 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/386911:G6N37_RS25770 ^@ http://purl.uniprot.org/uniprot/A0A7I7NSX9 ^@ Cofactor|||Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ Acts as a transcriptional regulator. Probably redox-responsive. The apo- but not holo-form probably binds DNA.|||Belongs to the WhiB family.|||Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.|||Cytoplasm|||The Fe-S cluster can be nitrosylated by nitric oxide (NO).|||Upon Fe-S cluster removal intramolecular disulfide bonds are formed. http://togogenome.org/gene/386911:G6N37_RS09665 ^@ http://purl.uniprot.org/uniprot/A0A7I7NXQ4 ^@ Function|||PTM|||Similarity|||Subcellular Location Annotation ^@ 4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.|||Acyl carrier protein involved in meromycolate extension.|||Belongs to the acyl carrier protein (ACP) family.|||Carrier of the growing fatty acid chain in fatty acid biosynthesis.|||Cytoplasm http://togogenome.org/gene/386911:G6N37_RS19335 ^@ http://purl.uniprot.org/uniprot/A0A7I7P4C8 ^@ Similarity ^@ Belongs to the WXG100 family. http://togogenome.org/gene/386911:G6N37_RS13720 ^@ http://purl.uniprot.org/uniprot/A0A7I7NXG2 ^@ Similarity ^@ Belongs to the WXG100 family. http://togogenome.org/gene/386911:G6N37_RS24100 ^@ http://purl.uniprot.org/uniprot/A0A7I7P640 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL2 family.|||One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.|||Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome. http://togogenome.org/gene/386911:G6N37_RS07225 ^@ http://purl.uniprot.org/uniprot/A0A7I7NWB5 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterioferritin family.|||Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.|||Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. http://togogenome.org/gene/386911:G6N37_RS24125 ^@ http://purl.uniprot.org/uniprot/A0A7I7P8C4 ^@ Similarity ^@ Belongs to the universal ribosomal protein uL29 family. http://togogenome.org/gene/386911:G6N37_RS13095 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0G3 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/386911:G6N37_RS06825 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1A6 ^@ Similarity ^@ Belongs to the WXG100 family. http://togogenome.org/gene/386911:G6N37_RS02240 ^@ http://purl.uniprot.org/uniprot/A0A7I7NTF1 ^@ Function|||Similarity ^@ Belongs to the bacterial ribosomal protein bL19 family.|||This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site. http://togogenome.org/gene/386911:G6N37_RS15155 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1I5 ^@ Similarity ^@ Belongs to the acyl-CoA dehydrogenase family. http://togogenome.org/gene/386911:G6N37_RS11170 ^@ http://purl.uniprot.org/uniprot/A0A7I7NZB8 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL21 family.|||Part of the 50S ribosomal subunit. Contacts protein L20.|||This protein binds to 23S rRNA in the presence of protein L20. http://togogenome.org/gene/386911:G6N37_RS02730 ^@ http://purl.uniprot.org/uniprot/A0A7I7NTR4 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS15 family.|||Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.|||Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA. http://togogenome.org/gene/386911:G6N37_RS18280 ^@ http://purl.uniprot.org/uniprot/A0A7I7P3B4 ^@ Similarity ^@ Belongs to the UPF0337 (CsbD) family. http://togogenome.org/gene/386911:G6N37_RS09055 ^@ http://purl.uniprot.org/uniprot/A0A7I7NYP8 ^@ Similarity ^@ Belongs to the YggT family. http://togogenome.org/gene/386911:G6N37_RS24080 ^@ http://purl.uniprot.org/uniprot/A0A7I7P622 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS10 family.|||Involved in the binding of tRNA to the ribosomes.|||Part of the 30S ribosomal subunit. http://togogenome.org/gene/386911:G6N37_RS23835 ^@ http://purl.uniprot.org/uniprot/A0A7I7P685 ^@ Function|||PTM|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL11 family.|||Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.|||One or more lysine residues are methylated.|||Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex. http://togogenome.org/gene/386911:G6N37_RS05595 ^@ http://purl.uniprot.org/uniprot/A0A7I7NWQ1 ^@ Function|||Similarity ^@ Belongs to the bacterial ribosomal protein bL20 family.|||Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit. http://togogenome.org/gene/386911:G6N37_RS01020 ^@ http://purl.uniprot.org/uniprot/A0A7I7NT40 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the ABC transporter superfamily.|||Cell membrane|||Homodimer. Forms a membrane-associated complex with FtsX.|||Part of the ABC transporter FtsEX involved in cellular division. http://togogenome.org/gene/386911:G6N37_RS04100 ^@ http://purl.uniprot.org/uniprot/A0A7I7NV53 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ Belongs to the elongation factor P family.|||Cytoplasm|||Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase. http://togogenome.org/gene/386911:G6N37_RS24120 ^@ http://purl.uniprot.org/uniprot/A0A7I7P635 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL16 family.|||Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.|||Part of the 50S ribosomal subunit. http://togogenome.org/gene/386911:G6N37_RS15065 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1Y0 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/386911:G6N37_RS25125 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6M7 ^@ Similarity|||Subcellular Location Annotation ^@ Belongs to the UPF0410 family.|||Membrane http://togogenome.org/gene/386911:G6N37_RS14870 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0R1 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/386911:G6N37_RS17635 ^@ http://purl.uniprot.org/uniprot/A0A7I7P2E5 ^@ Subcellular Location Annotation ^@ Cytoplasm http://togogenome.org/gene/386911:G6N37_RS24505 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6Z3 ^@ Function|||Similarity|||Subcellular Location Annotation|||Subunit ^@ Belongs to the IF-1 family.|||Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.|||Cytoplasm|||One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex. http://togogenome.org/gene/386911:G6N37_RS02220 ^@ http://purl.uniprot.org/uniprot/A0A7I7NVU6 ^@ Function|||Similarity|||Subcellular Location Annotation ^@ A probable RNA-binding protein.|||Belongs to the KhpA RNA-binding protein family.|||Cytoplasm http://togogenome.org/gene/386911:G6N37_RS15110 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1H6 ^@ Similarity ^@ Belongs to the short-chain dehydrogenases/reductases (SDR) family. http://togogenome.org/gene/386911:G6N37_RS13515 ^@ http://purl.uniprot.org/uniprot/A0A7I7P165 ^@ Function|||Similarity ^@ Belongs to the GreA/GreB family.|||Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. http://togogenome.org/gene/386911:G6N37_RS11165 ^@ http://purl.uniprot.org/uniprot/A0A7I7P1X7 ^@ Similarity ^@ Belongs to the bacterial ribosomal protein bL27 family. http://togogenome.org/gene/386911:G6N37_RS24745 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6N8 ^@ Similarity ^@ Belongs to the sigma-70 factor family. ECF subfamily. http://togogenome.org/gene/386911:G6N37_RS06820 ^@ http://purl.uniprot.org/uniprot/A0A7I7NXG2 ^@ Similarity ^@ Belongs to the WXG100 family. http://togogenome.org/gene/386911:G6N37_RS26270 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0Y2 ^@ Similarity ^@ Belongs to the thiolase-like superfamily. Thiolase family. http://togogenome.org/gene/386911:G6N37_RS23990 ^@ http://purl.uniprot.org/uniprot/A0A7I7P616 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS7 family.|||One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.|||Part of the 30S ribosomal subunit. Contacts proteins S9 and S11. http://togogenome.org/gene/386911:G6N37_RS19655 ^@ http://purl.uniprot.org/uniprot/A0A7I7P712 ^@ Function|||Similarity ^@ Belongs to the bacterial ribosomal protein bS6 family.|||Binds together with S18 to 16S ribosomal RNA. http://togogenome.org/gene/386911:G6N37_RS23905 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6M6 ^@ Function|||Similarity|||Subunit ^@ Belongs to the bacterial ribosomal protein bL12 family.|||Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.|||Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors. http://togogenome.org/gene/386911:G6N37_RS24195 ^@ http://purl.uniprot.org/uniprot/A0A7I7P657 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uL14 family.|||Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.|||Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8. http://togogenome.org/gene/386911:G6N37_RS23985 ^@ http://purl.uniprot.org/uniprot/A0A7I7P6B0 ^@ Function|||Similarity|||Subunit ^@ Belongs to the universal ribosomal protein uS12 family.|||Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.|||Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.|||With S4 and S5 plays an important role in translational accuracy. http://togogenome.org/gene/386911:G6N37_RS08815 ^@ http://purl.uniprot.org/uniprot/A0A7I7P0D7 ^@ Domain|||Function|||PTM|||Similarity|||Subunit ^@ Belongs to the prokaryotic ubiquitin-like protein family.|||Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process.|||Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation.|||Strongly interacts with the proteasome-associated ATPase ARC through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half. There is one Pup binding site per ARC hexamer ring.|||The N-terminal unstructured half of Pup provides a signal required to initiate unfolding and degradation by the proteasome but is not needed for pupylation, while the C-terminal helical half of Pup interacts with ARC to target proteins to the proteasome.