2-aminoethylphosphonate--pyruvate transaminase
Decreased affinity for all of the substrates.
Interaction with polymerase core subunit RpoC
Bacterial microcompartment shell protein PduJ
Produces amorphous bodies instead of BMCs. Reduces crystallographic problems that result from edge-to-edge aggregation of BMC hexamers. No longer forms filaments upon overexpression, does not restore BMC formation to a double pduA-pduJ deletion.
Wild-type growth rate, BMCs appear normal, wild-type DDH activity. In the chromosomal position of pduA, less permeable to 1,2-PD, 65% DDH activity, cells grow slower on 1,2-PD.
Ribonucleoside-diphosphate reductase 1 subunit beta
Confers streptomycin independence on the streptomycin-dependent mutant L-90.
Confers streptomycin independence on streptomycin-dependent S12 mutant P90R. A RAM mutant.
Confers streptomycin independence on streptomycin-dependent S12 mutant P91D. A RAM mutant.
In JB221; fast-growing suppressor of streptomycin resistant mutant S12 K42N. A RAM mutant.
Streptomycin resistant. Also confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
Streptomycin resistant. Also confers streptomycin independence on streptomycin-dependent S12 mutant P91D.
Flagellar hook-basal body complex protein FliE
N-acetylmuramic acid 6-phosphate etherase
Phosphocysteine intermediate; for EIIB activity
Abolishes function, decreased autophosphatase activity.
Abolishes function, reduced rate of phosphorylation and affinity for magnesium ion.
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Ribosomal RNA large subunit methyltransferase E
Almost completely abrogates the secretion of SptP and also affects the secretion of early (InvJ/SctP) and middle (SipB/SctE) substrates.
Can still secrete the T3SS components early substrates (InvJ/SctP), middle substrates (SipB/SctE, SipC/SctB and SipD/SctA), but shows a marked defect in the secretion of the effector proteins SptP and SopB (late substrates). Secretion of the effectors SipA and SopE2 is only marginally reduced.
Exists predominantly as monomer in solution and can self-associates into dimers.
Lack of ATPase activity. Defective in type III secretion and invasion of cultured cells.
Lack of ATPase activity. Exhibits a decreased ability to form oligomers. Defective in type III secretion and invasion of cultured cells.
Lack of ATPase activity. Severely defective for the secretion of early (InvJ/SctP), middle (SipB/SctE) and late (SptP) substrates.
Retains wild-type levels of ATPase activity. Defective in type III secretion and invasion of cultured cells.
Retains wild-type levels of ATPase activity. Defective in type III secretion and invasion of cultured cells. Does not bind the SicP-SptP complex.
Retains wild-type levels of ATPase activity. Drastically impaired in its ability to associate with the membrane. Defective in type III secretion and invasion of cultured cells.
Shows a defect in the secretion of SptP but not in the secretion of early (InvJ/SctP) or middle (SipB/SctE) substrates.
Probable [Fe-S]-dependent transcriptional repressor
Loss of ATP hydrolysis and autophosphorylation.
Uncharacterized MFS-type transporter YfcJ
Bacterial microcompartment shell protein PduU
Glutathione-regulated potassium-efflux system ancillary protein KefG
Flagella basal body P-ring formation protein FlgA
Phenylalanine--tRNA ligase alpha subunit
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Ribosomal RNA small subunit methyltransferase A
Type I restriction enzyme StySJI specificity subunit
Potassium-transporting ATPase ATP-binding subunit
Cytochrome c biogenesis ATP-binding export protein CcmA 2
Soluble pyridine nucleotide transhydrogenase
Thiosulfate reductase molybdopterin-containing subunit PhsA
Probable 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase 2
Aminodeoxychorismate synthase component 2
Autoinducer 2 import system permease protein LsrD
Probable amino-acid metabolite efflux pump
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
Vitamin B6 salvage pathway transcriptional repressor PtsJ
Ion-translocating oxidoreductase complex subunit C
Schiff-base intermediate with substrate; via pyruvic acid
Lipopolysaccharide export system protein LptA
Alpha-ketoglutarate-dependent dioxygenase AlkB
Imidazole glycerol phosphate synthase subunit HisH
Outer membrane protein assembly factor BamE
Transposase for insertion sequence element IS200
Spermidine/putrescine import ATP-binding protein PotA
Transcription termination/antitermination protein NusA
5-glutamyl coenzyme A thioester intermediate
Maltose/maltodextrin-binding periplasmic protein
Ubiquinone biosynthesis accessory factor UbiK
Acyl-ester intermediate; for transpeptidase activity
Proton donor; for transglycosylase activity
Uncharacterized inner membrane transporter YicL
Probable HTH-type transcriptional regulator SinR
Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
Increases ATPase-inducing activity and histidine transport; when associated with K-47.
Slight decrease in ATPase-inducing activity and histidine transport.
Strong decrease in ATPase-inducing activity and histidine transport.
Low molecular weight protein-tyrosine-phosphatase Wzb
Surface presentation of antigens protein SpaO
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Oxygen-dependent coproporphyrinogen-III oxidase
Periplasmic nitrate reductase, electron transfer subunit
4-hydroxyphenylacetate 3-monooxygenase reductase component
Peptide methionine sulfoxide reductase MsrA
Probable HTH-type transcriptional regulator LeuO
Anaerobic glycerol-3-phosphate dehydrogenase subunit B
Important for interaction with partner proteins
23S rRNA (uracil(747)-C(5))-methyltransferase RlmC
Signal transduction histidine-protein kinase/phosphatase UhpB
Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252)
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-105)
Unable to grow in minimal glucose medium; requires thiamine for growth. Is defective in FAD binding.
Lipopolysaccharide biosynthesis protein RfbH
Resolvase/invertase-type recombinase catalytic
Lipoprotein-releasing system ATP-binding protein LolD
Putative colanic acid biosynthesis glycosyltransferase WcaL
PTS system glucose-specific EIIA component
Tele-phosphohistidine intermediate; for EIIA activity
Molybdenum import ATP-binding protein ModC
(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase
Guanine nucleotide exchange factor sopE2
In acr-4 and acr-3 respectively; higher levels of cAMP synthesis.
cAMP-activated global transcriptional regulator CRP
Thiol:disulfide interchange protein DsbC
Oxygen-insensitive NAD(P)H nitroreductase
Fumarate and nitrate reduction regulatory protein
Sulfate transport system permease protein CysT
In gyrA209TS; a temperature-sensitive mutant, 15% decreased growth rate, decreased (-) supercoiling even at permissive temperature.
In gyrA213TS; a temperature-sensitive mutant, decreased (-) supercoiling even at permissive temperature.
O-(5'-phospho-DNA)-tyrosine intermediate
Sugar fermentation stimulation protein A
Ribosomal RNA large subunit methyltransferase I
FMN-dependent NADH:quinone oxidoreductase
tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydroxylase
Thiol:disulfide interchange protein DsbL
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Cyclic pyranopterin monophosphate synthase
High frequency lysogenization protein HflD homolog
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Adenosylcobinamide-GDP ribazoletransferase
Ascorbate-specific PTS system EIIA component
Thiol:disulfide interchange protein DsbA
Thiol:disulfide interchange protein DsbE
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Does not affect regulation of macrophage migration.
Loss of activity. No change in IQGAP1 binding.
Acetaldehyde dehydrogenase (acetylating) EutE
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
DNA-directed RNA polymerase subunit beta
In isoform Soluble copper chaperone CopA(Z).
Cysteine sulfenic acid (-SOH) intermediate
Probable cobalt-factor III C(17)-methyltransferase
Oligopeptide transport ATP-binding protein OppF
Spermidine/putrescine transport system permease protein PotB
Lipopolysaccharide core heptose(II) kinase RfaY
Peptide transport system ATP-binding protein SapD
Putative membrane-bound redox modulator Alx
Aminodeoxychorismate synthase component 1
N6-(4-deoxychorismate)-lysine intermediate
3,4-dihydroxy-2-butanone 4-phosphate synthase
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Kdo(2)-lipid A phosphoethanolamine 7''-transferase
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE
Complements deletion as well as wild-type.
Slight reduction in complementation of deletion.
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
2-iminobutanoate/2-iminopropanoate deaminase
Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
Redox-sensitive transcriptional activator SoxR
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Putative D-alanyl-D-alanine carboxypeptidase
p-hydroxybenzoic acid efflux pump subunit AaeA
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
Increases geranylation activity. Lack of geranylation activity; when associated with A-155.
Reduces the level of geranylation but does not abolish it.
Type III restriction-modification enzyme StyLTI Res subunit
O-(phosphoribosyl dephospho-coenzyme A)serine
Cytochrome c-type biogenesis protein CcmE
Helical; Signal-anchor for type II membrane protein
Membrane-bound lytic murein transglycosylase F
Secretion system apparatus lipoprotein SsaJ
Negative modulator of initiation of replication
Surface presentation of antigens protein SpaP
1000-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
2-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
750-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
Inactive. Retains the same oligomeric state of the wild-type.
Phosphate import ATP-binding protein PstB
Glutathione transport system permease protein GsiC
High-affinity zinc uptake system protein ZnuA
Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK
D-alanyl-D-alanine carboxypeptidase DacD
Confers streptomycin independence on streptomycin-dependent S12 mutant P90L.
In strain: JB279; fast-growing suppressor of streptomycin resistant S12 mutant K42N.
Bacterial microcompartment shell protein EutS
EutC and EutE constructs are no longer targeted to EutS or eutSMNLK BMCs. BMCs are formed in E.coli by both sets of genes.
EutC constructs are targeted to EutS BMCs, cells are somewhat elongated.
No longer targets EutC-tagged constructs to BMCs, probably forms flat symmetric hexamers.
Spermidine/putrescine-binding periplasmic protein
Bifunctional polymyxin resistance protein ArnA
Proton acceptor; for decarboxylase activity
Proton donor; for decarboxylase activity
Proton donor; for formyltransferase activity
Probable csgAB operon transcriptional regulatory protein
L-methionine sulfoximine/L-methionine sulfone acetyltransferase
Aspartate carbamoyltransferase catalytic subunit
Adenosylcobalamin/alpha-ribazole phosphatase
N-acetyl-gamma-glutamyl-phosphate reductase
Loss of kinase activity. No effect on translocation of the protein.
Putative 2-aminoethylphosphonate transport system permease protein PhnV
Membrane-bound lytic murein transglycosylase C
High-affinity branched-chain amino acid transport system permease protein LivH
Probable transcriptional regulatory protein YeeN
Ion-translocating oxidoreductase complex subunit A
Iron-sulfur cluster insertion protein ErpA
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Cobalt-precorrin-2 C(20)-methyltransferase
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Outer membrane protein assembly factor BamA
Oxygen-independent coproporphyrinogen III oxidase
UDP-N-acetylenolpyruvoylglucosamine reductase
10-fold reduced MIC for streptomycin, no change in spectinomycin resistance, reduced streptomycin but not spectinomycin binding.
2.7-fold reduced MIC for streptomycin, loss of spectinomycin resistance, no change in ATP or antibiotic binding.
4-5-fold reduced MIC for streptomycin and spectinomycin, very little spectinomycin binding.
5-fold reduced MIC for streptomycin, 1.5-fold reduced MIC for spectinomycin resistance, reduced streptomycin but not spectinomycin binding.
8-fold reduced MIC for streptomycin, loss of spectinomycin resistance.
Aminoglycoside (3'') (9) adenylyltransferase
Loss of streptomycin and spectinomycin resistance, 4-fold decreased ATP-binding, significantly decreased streptomycin binding, no spectinomycin binding.
Loss of streptomycin and spectinomycin resistance, no ATP-binding, near wild-type streptomycin binding, significantly decreased spectinomycin binding.
Loss of streptomycin and spectinomycin resistance, no ATP-binding, very little antibiotic binding.
Propanediol dehydratase-reactivating factor small subunit
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Undecaprenyl-phosphate galactose phosphotransferase
Leucyl/phenylalanyl-tRNA--protein transferase
Galactose/methyl galactoside import ATP-binding protein MglA
Thiamine transport system permease protein ThiP
RNA polymerase-binding transcription factor DksA
Allows movement of 1,2-PD and NAD(+)/NADH across the BMC, excretes slightly more propionaldehyde, makes normal BMCs.
Bacterial microcompartment shell protein PduT
No transport of 1,2-PD or NAD(+)/NADH across the BMC, makes normal BMCs.
No transport of 1,2-PD or NAD(+)/NADH across the BMC, no change in propionaldehyde excretion, makes normal BMCs.
Purified protein is more stable, crystallized, has a triangular pore of about 46 Angstroms(2) area. Allows movement of 1,2-PD and NAD(+)/NADH across the BMC, excretes slightly more propionaldehyde, makes normal BMCs.
Negative regulator of flagellin synthesis
2-dehydro-3-deoxyphosphooctonate aldolase
Surface presentation of antigens protein SpaS
Magnesium and cobalt efflux protein CorC
Acetolactate synthase isozyme 3 small subunit
Succinate dehydrogenase cytochrome b556 subunit
Glucose-1-phosphate cytidylyltransferase
Thiosulfate reductase electron transfer subunit PhsB
Localizes almost exclusively to the cytosol of transfected cells.
Ion-translocating oxidoreductase complex subunit D
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Ethanolamine ammonia-lyase small subunit
Outer membrane protein assembly factor BamD
Nucleoside-specific channel-forming protein Tsx
High-affinity branched-chain amino acid transport ATP-binding protein LivF
Crossover junction endodeoxyribonuclease RuvC
Histidine transport system permease protein HisQ
Lipopolysaccharide core biosynthesis protein RfaZ
Does not complement deletion, 0.6% activity in vitro. No metal cofactor.
Does not complement deletion, 1.2% activity in vitro. No metal cofactor.
Does not complement deletion, 20% activity in vitro. Reduces metal content of enzyme, complements a deletion.
No effect in vivo or in vitro. No binding of Co(2+).
Reduces metal content of enzyme, does not complement deletion. No binding of Co(2+).
Potassium-transporting ATPase KdpC subunit
Considerably reduced DNA-binding, in fragment 91-137.
Loss of DNA-binding, in fragment 91-137.
No change in DNA-binding, in fragment 91-137.
No effect on DNA-binding or physical properties.
No longer binds Hha or YdgT, slightly altered DNA-binding, may self associate into longer than wild-type filaments. Derepression.
No longer binds Hha or YdgT, slightly altered DNA-binding, wild-type self-association. Derepression of some H-NS-regulated genes, acts similarly to an hha deletion strain.
No longer binds Hha, still able to bind YdgT.
Reduced DNA-binding, in fragment 91-137.
Dual-specificity RNA methyltransferase RlmN
NAD-dependent dihydropyrimidine dehydrogenase subunit PreT
Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)
Peptidyl-lysine N-acetyltransferase YjaB
Pyridoxine/pyridoxamine 5'-phosphate oxidase
Cysteine sulfinic acid (-SO2H); alternate
HTH-type transcriptional repressor STM4068
Enzyme no longer associated with BMCs, no effect on enzyme activity.
Branched-chain-amino-acid aminotransferase
Loss of activity at elevated temperature.
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Ion-translocating oxidoreductase complex subunit B
tRNA1(Val) (adenine(37)-N6)-methyltransferase
Phosphoribosylaminoimidazole-succinocarboxamide synthase
ATP-dependent dethiobiotin synthetase BioD 2
Pros-phosphohistidine intermediate; for HPr activity
3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase
Much less protein associates with BMCs, no effect on enzyme activity.
Propanal dehydrogenase (CoA-propanoylating)
Substantially decreased protein levels in BMCs, no change in enzyme activity.
Glycine betaine/proline betaine transport system permease protein ProW
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
Methylated-DNA--protein-cysteine methyltransferase
Peptide methionine sulfoxide reductase MsrB
Holliday junction ATP-dependent DNA helicase RuvA
UDP-3-O-acyl-N-acetylglucosamine deacetylase
Large ribosomal RNA subunit accumulation protein YceD
Glutathione-regulated potassium-efflux system protein KefC
Lipopolysaccharide heptosyltransferase 1
Curli production assembly/transport component CsgE
Cytochrome c biogenesis ATP-binding export protein CcmA 1
Ribosomal RNA large subunit methyltransferase F
Phosphoenolpyruvate-dependent phosphotransferase system
Thiamine import ATP-binding protein ThiQ
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
6-phosphogluconate dehydrogenase, decarboxylating
Carboxy-S-adenosyl-L-methionine synthase
Galactose/methyl galactoside import permease protein MglC
Glucans biosynthesis glucosyltransferase H
Acetolactate synthase isozyme 3 large subunit
sn-glycerol-3-phosphate-binding periplasmic protein UgpB
Type 1 fimbrin D-mannose specific adhesin
5-fold decrease in deacylase activity. Does not affect translocation by TTSS and cellular localization.
Fatty acid oxidation complex subunit alpha
Decrease in activity. Defect in histidine binding.
UDP-N-acetyl-D-mannosaminuronic acid transferase
Ascorbate-specific PTS system EIIB component
Dual-specificity RNA pseudouridine synthase RluF
Galactose-1-phosphate uridylyltransferase
Glutathione-regulated potassium-efflux system protein KefB
Putative transcriptional regulator of 2-aminoethylphosphonate degradation operons
Endo-type membrane-bound lytic murein transglycosylase A
Trk system potassium uptake protein TrkA
Ion-translocating oxidoreductase complex subunit G
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
Formate hydrogenlyase transcriptional activator
Aerobic C4-dicarboxylate transport protein
Flagellar transcriptional regulator FlhD
Acetylornithine/succinyldiaminopimelate aminotransferase
Iron-sulfur cluster assembly protein CyaY
Autoinducer 2 import system permease protein LsrC
Alters interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-130.
Fails to induce host endosomal tubulation; when associated with A-197.
Fails to induce host endosomal tubulation; when associated with A-201.
Loss of interaction with host PLEKHM2. Fails to induce host endosomal tubulation; when associated with D-131.
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase
Methyl-accepting chemotaxis citrate transducer
Gly-cisPro motif, important for rejection of L-amino acids
SPI-1 type 3 secretion system stator protein
ATP-dependent Clp protease ATP-binding subunit ClpX
3'(2'),5'-bisphosphate nucleotidase CysQ
Ribosomal large subunit pseudouridine synthase C
Impaired helical structure and abolished catalytic activity.
UDP-N-acetyl-D-mannosamine dehydrogenase
3-keto-L-gulonate-6-phosphate decarboxylase UlaD
Basal-body rod modification protein FlgD
Putative surface-exposed virulence protein BigA
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
Pyridoxine/pyridoxal/pyridoxamine kinase
Multiple antibiotic resistance protein MarR
Lipopolysaccharide 1,6-galactosyltransferase
Pyruvate dehydrogenase complex repressor
Probable replication endonuclease from prophage-like region
Uncharacterized MFS-type transporter YcaD
5,10-methylenetetrahydrofolate reductase
Histidine biosynthesis bifunctional protein HisIE
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Maltose/maltodextrin transport system permease protein MalF
Penicillin-binding protein activator LpoB
Surface presentation of antigens protein SpaQ
Sec-independent protein translocase protein TatB
DNA-binding dual transcriptional regulator OmpR
Probable Sec-independent protein translocase protein TatE
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
3-isopropylmalate dehydratase small subunit 2
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Purine nucleoside phosphorylase DeoD-type
Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
Ribosomal RNA large subunit methyltransferase G
Vitamin B12 import ATP-binding protein BtuD
UTP--glucose-1-phosphate uridylyltransferase
In parE206TS; a temperature-sensitive mutant, 33% decreased growth rate, slightly decreased negative supercoiling at permissive temperature.
In temperature-sensitive mutant parE377.
In temperature-sensitive mutant parE493.
In temperature-sensitive mutants parE206 and parE374.
Complete loss of NMN adenylyltransferase activity.
No NMN adenylyltransferase activity. Binds DNA independently of NAD.
No NMN adenylyltransferase activity. Binds DNA independently of NAD; when associated with H-123.
No NMN adenylyltransferase activity. Binds DNA independently of NAD; when associated with N-173.
Trifunctional NAD biosynthesis/regulator protein NadR
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
Cell division topological specificity factor
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Putative 2-aminoethylphosphonate-binding periplasmic protein
Succinate--CoA ligase [ADP-forming] subunit beta
Complements a cobB deletion, produces only the long isoform. Has deacylation and phosphoribosyltransferase activities.
Complements a cobB deletion, produces only the short isoform. Has deacylation and phosphoribosyltransferase activities.
Ascorbate-specific PTS system EIIC component
Phosphoenolpyruvate-protein phosphotransferase
Phosphoglycerate transport regulatory protein PgtC
Expression at concentrations above 30 uM retains a protein-protein interaction and PhoQ-independent induction of PAG. Yields insoluble purified protein above 7 uM.
Virulence transcriptional regulatory protein PhoP
No localization with flagellar basal body, thus no binding to FliM.
Suppresses a yhjH disruption mutant upon overexpression, binds FliM but no longer affects motility in this assay.
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
Intracellular growth attenuator protein IgaA
Ubiquinone biosynthesis accessory factor UbiJ
SPI-2 type 3 secretion system stator protein
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
Malonyl CoA-acyl carrier protein transacylase
In strain: RKS2985, RKS2993, RKS2995 and RKS3057.
In strain: RKS3013, RKS3014 and RKS3015.
ATP-dependent Clp protease proteolytic subunit
3-isopropylmalate dehydratase small subunit 1
Bacterial microcompartment shell protein EutL
O antigen biosynthesis rhamnosyltransferase RfbN
Peptide transport system permease protein SapC
Ribosomal RNA small subunit methyltransferase H
2-fold decrease in enzymatic activity, but no effect on substrate affinity.
20-fold decrease in enzymatic activity, but no effect on substrate affinity.
47-fold decrease in enzymatic activity, but no effect on substrate affinity.
7-fold decrease in enzymatic activity, but no effect on substrate affinity.
More than 5-fold decrease in substrate affinity.
Nearly no decrease in enzymatic activity.
Glucose-1-phosphate thymidylyltransferase
Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.
Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
In parC281TS; a temperature-sensitive mutant, no change in growth rate or negative supercoiling at permissive temperature.
Pimeloyl-[acyl-carrier protein] methyl ester esterase
Alters specificity to use phenolic compounds as substrate; when associated with M-88 and M-175.
Alters specificity to use phenolic compounds as substrate; when associated with Y-80 and M-175.
Alters specificity to use phenolic compounds as substrate; when associated with Y-80 and M-88.
Decreases specific activity for DMB and adenine about 20-fold. Decreases specific activity 1600- to 15000-fold; when associated with A-317.
Decreases specific activity for DMB and adenine about 50-fold; crystals bind substrate less well. Decreases specific activity 1600- to 15000-fold; when associated with A-174.
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Ribosomal RNA small subunit methyltransferase F
Non-specific ribonucleoside hydrolase RihC
Can bind and transfer an Fe-S cluster, but has no detectable ATPase activity.
Can hydrolyze ATP and transfer an Fe-S cluster.
Hydrolyzes ATP and can bind an Fe-S cluster, but cluster transfer is compromised.
Schiff-base intermediate with acetaldehyde
Autoinducer 2 import ATP-binding protein LsrA
Succinate dehydrogenase iron-sulfur subunit
Bifunctional purine biosynthesis protein PurH
Mannose-1-phosphate guanylyltransferase ManC
Aminoalkylphosphonate N-acetyltransferase
Sensory histidine kinase/phosphatase NtrB
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Periplasmic serine endoprotease DegP-like
Ribonucleoside-diphosphate reductase 1 subunit alpha
Type III restriction-modification enzyme StyLTI Mod subunit
Almost no change in feedback control by tryptophan.
Complete loss of feedback control by tryptophan.
Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.
Decrease in feedback control by tryptophan.
Uncharacterized MFS-type transporter STM4428
Methionine import ATP-binding protein MetN 2
Glyceraldehyde-3-phosphate dehydrogenase
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
Ribosomal RNA large subunit methyltransferase H
Putative N-acetylmannosamine-6-phosphate 2-epimerase 1
3-methyl-2-oxobutanoate hydroxymethyltransferase
Aromatic amino acid transport protein AroP
Glycine betaine/proline betaine transport system ATP-binding protein ProV
Propionate catabolism operon regulatory protein
Glutamate-1-semialdehyde 2,1-aminomutase
Surface presentation of antigens protein SpaM
Transcription termination/antitermination protein NusG
Xanthine-guanine phosphoribosyltransferase
tRNA N6-adenosine threonylcarbamoyltransferase
Curli production assembly/transport component CsgF
Outer-membrane lipoprotein carrier protein
Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ
serine-type D-Ala-D-Ala carboxypeptidase
3-isopropylmalate dehydratase large subunit 2
Homoserine/homoserine lactone efflux protein
NAD-dependent dihydropyrimidine dehydrogenase subunit PreA
Macrolide export ATP-binding/permease protein MacB
Peptide transport system ATP-binding protein SapF
Ethanolamine ammonia-lyase reactivase EutA
Loss of covalent pyridoxal phosphate binding.
UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase
Localizes throughout the cytosol and not to late endocytic compartments; when associated with P-37.
Localizes throughout the cytosol and not to late endocytic compartments; when associated with R-44.
Required to target late endocytic compartments
Anaerobic ribonucleoside-triphosphate reductase
Lipopolysaccharide core heptose(I) kinase RfaP
sn-glycerol-3-phosphate transport system permease protein UgpA
Holliday junction ATP-dependent DNA helicase RuvB
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
Aspartate carbamoyltransferase regulatory chain
Alters cation selectivity. Decreases H(+)- and Na(+)-coupled activity, with little effect on Li(+)-coupled melibiose transport.
Alters cation selectivity. Inhibits H(+)- and Na(+)-coupled activity, with little effect on Li(+)-coupled melibiose transport.
Alters cation selectivity. Loss of transporter activity.
Alters cation selectivity. Retains a low level of H(+)-coupled melibiose transport, but retains only a weak activity with Na(+) or Li(+).
Alters cation selectivity. Retains only a low level of H(+)-coupled melibiose binding and active transport, but Na(+) or Li(+) does not stimulate either binding or transport.
Inhibits Na(+)- and Li(+)-coupled activity, with little effect on H(+)-coupled melibiose transport.
Retains weak activity with Na(+), Li(+) or H(+).
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Chromosomal replication initiator protein DnaA
Uncharacterized N-acetyltransferase YjgM
Considerably decreased amount of PduL in BMCs, enzyme has greater than wild-type activity.
Decreased amount of PduL in BMCs, enzyme has wild-type activity.
Stationary-phase-induced ribosome-associated protein
Ribosomal RNA small subunit methyltransferase B
Propanediol dehydratase-reactivating factor large subunit
ATP-dependent dethiobiotin synthetase BioD 1
Molybdopterin-synthase adenylyltransferase
Glycine betaine/proline betaine-binding periplasmic protein
HTH-type transcriptional regulatory protein TyrR
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Ribosomal large subunit pseudouridine synthase D
Endolytic peptidoglycan transglycosylase RlpA
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Release factor glutamine methyltransferase
Ion-translocating oxidoreductase complex subunit E
Peptide transport system permease protein SapB
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
Lack of ATPase activity. Cannot dissociate SseB/SsaE complex.
Retains ATPase activity, but does not interact with the chaperone SrcA. Strong virulence attenuation phenotype.
Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)
Inhibitor of hydrogen peroxide resistance
In gyrB1820TS; a temperature-sensitive mutant, 50% decreased growth rate, greatly decreased (-) supercoiling even at permissive temperature.
In gyrB652TS; a temperature-sensitive mutant, no growth at 42 degrees Celsius, 36% decreased growth rate at 30 degrees Celsius (permissive), low k(cat) at 30, 37 and 42 degrees Celsius, in vivo significantly decreased (-) supercoiling even at permissive temperature.
Imidazole glycerol phosphate synthase subunit HisF
Nitric oxide reductase FlRd-NAD(+) reductase
Maltose/maltodextrin transport system permease protein MalG
Sulfite reductase [NADPH] flavoprotein alpha-component
Oligopeptide transport system permease protein OppC
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Lipopolysaccharide 1,3-galactosyltransferase
Histidine transport system permease protein HisM
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
DNA-directed RNA polymerase subunit alpha
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
PduOC is no longer red, the mutation in the whole protein does not complement as well as wild-type.
Small ribosomal subunit biogenesis GTPase RsgA
Putative glucose-6-phosphate 1-epimerase
Formate-dependent phosphoribosylglycinamide formyltransferase
Ribonucleoside-diphosphate reductase 2 subunit beta
D-tagatose-1,6-bisphosphate aldolase subunit GatZ
Ribosomal protein S12 methylthiotransferase RimO
Arylamine N-acetyltransferase / N-hydroxyarylamine O-acetyltransferase
Loss of O- and N-acetyltransferase activities.
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
Glutathione-regulated potassium-efflux system ancillary protein KefF
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
sn-glycerol-3-phosphate transport system permease protein UgpE
Osmoprotectant import permease protein OsmW
Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive
Ribosomal RNA large subunit methyltransferase M
Nicotinate-nucleotide adenylyltransferase
Ribosomal RNA small subunit methyltransferase J
Reduced enzymatic activity and poor motility.
Reduced enzymatic activity and poor motility; strongly reduced enzymatic activity and severely attenuated motility; when associated with N-248.
Dual-specificity RNA pseudouridine synthase RluA
Sulfate/thiosulfate import ATP-binding protein CysA
Transcription antitermination protein NusB
Loss of activity. Cannot bind acetyl-CoA. Does not interact with pro-PanD.
Histidine biosynthesis bifunctional protein HisB
Outer membrane protein assembly factor BamB
Beta-ketoacyl-[acyl-carrier-protein] synthase III
Mannose-1-phosphate guanylyltransferase RfbM
Putative 2-aminoethylphosphonate import ATP-binding protein PhnT
Probable L-ascorbate-6-phosphate lactonase UlaG
Phosphoglycerate transport system sensor protein PgtB
Glutamate/aspartate import solute-binding protein
ATP-dependent protease ATPase subunit HslU
Low level streptomycin, spectinomycin and kanamycin resistance, has small colony phenotype.
Pyrimidine/purine nucleoside phosphorylase
In SS-C; sensitive to hydrophobic antibiotics and compounds.
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Reduces the epimerase activity by over 100-fold.
Reduces the epimerase activity by over 1000-fold.
Polyribonucleotide nucleotidyltransferase
PTS system glucose-specific EIICB component
Abolishes formation of s(2)C32; when associated with A-122.
Abolishes formation of s(2)C32; when associated with A-125.
O-(3'-phospho-DNA)-tyrosine intermediate
1-acyl-sn-glycerol-3-phosphate acyltransferase
Putative N-acetylmannosamine-6-phosphate 2-epimerase 2
Succinate dehydrogenase flavoprotein subunit
Osmoprotectant import permease protein OsmY
Virulence genes transcriptional activator
23S rRNA (guanosine-2'-O-)-methyltransferase RlmB
Nucleophile; methyl group acceptor from phosphotriester
Tetrathionate response regulatory protein TtrR
Penicillin-binding protein activator LpoA
3-oxoacyl-[acyl-carrier-protein] reductase FabG
Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
Loss of the temperature-sensitive phenotype; when associated with I-125.
Loss of the temperature-sensitive phenotype; when associated with T-223.
Methionine import ATP-binding protein MetN 1
Low level streptomycin and spectinomycin resistance.
Ribosomal RNA small subunit methyltransferase G
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
DNA replication terminus site-binding protein
Ribosomal small subunit pseudouridine synthase A
Ubiquinone biosynthesis O-methyltransferase
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
Penicillin-insensitive murein endopeptidase
Type III secretion system chaperone SseA
Recombination-promoting nuclease pSLT051
Phosphoenolpyruvate synthase regulatory protein
Surface presentation of antigens protein SpaR
Hemolysin expression-modulating protein Hha
No longer interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
Still interacts with H-NS, derepresses expression of H-NS/Hha coregulated genes but not genes regulated solely by H-NS.
Tail fiber assembly protein homolog from lambdoid prophage Fels-1
Anaerobic nitric oxide reductase flavorubredoxin
High-affinity branched-chain amino acid transport system permease protein LivM
Multiple antibiotic resistance protein MarA
Abolishes enzyme activity and 1.5-fold reduction of the affinity for 3-dehydroquinate.
Strong reduction of the catalytic efficiency and slight increase of the affinity for 3-dehydroquinate.
Very strong reduction of the catalytic efficiency and almost the same affinity for 3-dehydroquinate.
Protein-methionine-sulfoxide reductase catalytic subunit MsrP
Osmoprotectant import ATP-binding protein OsmV
Protoporphyrinogen IX dehydrogenase [quinone]
Single-stranded-DNA-specific exonuclease RecJ
Ethanolamine ammonia-lyase large subunit
Lipopolysaccharide core heptose(II)-phosphate phosphatase
Phosphoribosylformylglycinamidine synthase
This mutant has a lower overall folding of the secondary structure and shows a 60% loss in FGAM synthase activity.
This mutant has the same secondary structure content and FGAM synthetase activity as the wild-type protein and exhibits almost no destabilization.
This mutant is extremely deleterious to the structural integrity of the protein.
This mutant is structurally identical to the wild-type protein.
This mutant shows a disturbance in the secondary structure of the protein and causes a 30% loss in FGAM synthase activity.
This mutant shows a perturbation of the local environment, however has a secondary structure content and a FGAM synthase activity very similar to the wild-type protein.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Putative multidrug resistance protein MdtD
Ribosomal RNA large subunit methyltransferase K/L
No inhibition of cell growth, no degradation of tRNA(fMet).
Cobalt-precorrin-5B C(1)-methyltransferase
Translocation and assembly module subunit TamA
Carbamoyl-phosphate synthase large chain
Altered motor bias with clockwise rotation, partially suppresses a yhjH disruption.
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
NADP-dependent 3-hydroxy acid dehydrogenase YdfG
In mutants SL1102 and SL1027; heptoseless LPS.
Enzyme associates with BMCs, 25% decrease in enzyme activity.
Malonyl-[acyl-carrier protein] O-methyltransferase
Confers to cells a PhoP constitutively active phenotype. Affects PhoP/PhoQ-signaling cascade and increases net phosphorylation of PhoP. No effect on initial rate of autophosphorylation and decreases phosphatase activity.
Confers to cells a PhoP constitutively inactive phenotype. Affects PhoP/PhoQ-signaling cascade but no significantly effect on net phosphorylation of PhoP. Decreases 3-fold initial rate of autophosphorylation and increases phosphatase activity.
Confers to cells a PhoP wild-type phenotype. No effect on net phosphorylation of PhoP. Decreases 5-fold initial rate of autophosphorylation and increases phosphatase activity.
Confers to cells a PhoP wild-type phenotype. No effect on net phosphorylation of PhoP. Decreases 5-fold initial rate of autophosphorylation and shows a wild-type phosphatase activity.
Impaired PhoP repression by high concentrations of divalent cation.
Impaired PhoP repression by high concentrations of divalent cations.
In pho-24; low affinity for Ca(2+). Confers to cells a PhoP constitutively active phenotype. Affects PhoP/PhoQ-signaling cascade and increase net phosphorylation of PhoP. No effect on initial rate of autophosphorylation and decreases phosphatase activity.
Retains a wild-type Mg(2+) response only at 10 mM in strain PhoP* PhoQ expressing mutant phoP N-93. Loss of autophosphorylation, irrespective of the presence of Mg(2+). Unable to promote phoP dephosphorylation even in presence of added Mg(2+).
p-hydroxybenzoic acid efflux pump subunit AaeB
Abolishes plasmid resolution but not chromosomal recombination.
Oxaloacetate decarboxylase gamma chain 3
Surface presentation of antigens protein SpaK
TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase
Ribosomal RNA small subunit methyltransferase C
Peptide transport periplasmic protein SapA
Iron(3+)-hydroxamate import system permease protein FhuB
Molybdopterin synthase catalytic subunit
Ribosomal large subunit pseudouridine synthase B
Biosynthetic peptidoglycan transglycosylase
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
No acetyl-coenzyme A synthetase activity.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
Probable protein adenylyltransferase Fic
Bacterial microcompartment shell protein EutM
No longer complements pduA in the pdu operon.
Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF
Protein-arginine N-acetylglucosaminyltransferase SseK2
3-phosphoshikimate 1-carboxyvinyltransferase
Reduces affinity for magnesium about 10-fold. Reduces magnesium transport by about 98%.
Reduces magnesium transport by about 95%.
Reduces magnesium transport by about 99%.
ATP-dependent Clp protease adapter protein ClpS
Medium/long-chain acyl-CoA thioesterase YigI
Sulfite reductase [NADPH] hemoprotein beta-component
Bacterial microcompartment shell protein EutK
4-hydroxy-tetrahydrodipicolinate reductase
Ornithine carbamoyltransferase, catabolic
3-deoxy-manno-octulosonate cytidylyltransferase
BMCs have a severe assembly defect, makes about 27% BMCs.
Bacterial microcompartment shell protein PduB
Does not make PduB', makes about 66% BMCs, with wild-type appearance.
Makes about 25% BMCs, 35% diol dehydratase content.
Makes about 70% BMCs, 74% diol dehydratase content.
Makes about 80% BMCs, 78% diol dehydratase content.
Makes about 90% BMCs, 87% diol dehydratase content.
Makes large empty BMCs, makes about 11% BMCs.
Makes large empty BMCs, makes about 30% BMCs.
23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD
Large-conductance mechanosensitive channel
Tetrathionate sensor histidine kinase TtrS
Multiple antibiotic resistance protein MarB
Putative polysaccharide export protein wza
Defective in mismatch repair; ATPase activity reduced 6-fold.
Histidine transport ATP-binding protein HisP
NADPH-dependent 7-cyano-7-deazaguanine reductase
tRNA(Met) cytidine acetyltransferase TmcA
Ribosomal large subunit pseudouridine synthase E
Cobalt-precorrin-4 C(11)-methyltransferase
Elongation factor P--(R)-beta-lysine ligase
L-carnitine/gamma-butyrobetaine antiporter
Putative glutamine amidotransferase-like protein YfeJ
50S ribosomal subunit assembly factor BipA
No longer associates with 70S ribosomes.
No longer binds to 70S ribosomes in presence of GMPPNP, altered secondary structure.
No longer binds to 70S ribosomes in presence of GMPPNP.
No longer binds to 70S ribosomes, has no GTPase activity.
Still binds to 70S ribosomes in presence of GMPPNP.
Bacterial microcompartment shell vertex protein PduN
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Decreases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2.
Increases LAMP-1 redistribution. No effect on peripheral accumulation of GFP-PipB2.
Partial reduction in the peripheral accumulation of GFP-PipB2 and LAMP-1.
Prevents LE/Lys redistribution. Does not affect the peripheral accumulation of GFP-PipB2.
Prevents the peripheral accumulation of GFP-PipB2 and LAMP-1. Slightly affects its LE/Lys redistribution.
Abolished protein-arginine N-acetylglucosaminyltransferase activity.
N-beta-linked (GlcNAc) arginine; by autocatalysis
Protein-arginine N-acetylglucosaminyltransferase SseK1
Aminoglycoside N(6')-acetyltransferase type 1
Surface presentation of antigens protein SpaN
Trk system potassium uptake protein TrkH
Increases speed of forward and back reactions by 2-3 fold.
Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH.
Slightly decreases speed of forward and back reactions.
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
tRNA/tmRNA (uracil-C(5))-methyltransferase
Oxaloacetate decarboxylase gamma chain 1
Curli production assembly/transport component CsgG
Cobalt-precorrin-7 C(5)-methyltransferase
D-galactose/methyl-galactoside binding periplasmic protein MglB
sn-glycerol-3-phosphate import ATP-binding protein UgpC
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
Isocitrate dehydrogenase kinase/phosphatase
Anaerobic ribonucleoside-triphosphate reductase-activating protein
In strain: LT2 / SGSC1412 and S4194; resistance to polymyxin B and neutrophil proteins.
Carbamoyl-phosphate synthase small chain
Periplasmic oligopeptide-binding protein
Oxaloacetate decarboxylase gamma chain 2
Sec-independent protein translocase protein TatA
Low affinity potassium transport system protein Kup
Pantothenate precursors transporter PanS
Protein-glutamate methylesterase/protein-glutamine glutaminase
Slight increase in methylesterase activity.
Salmonella pathogenicity island 2 protein C
D-tagatose-1,6-bisphosphate aldolase subunit GatY
Putative membrane protein insertion efficiency factor
DNA-directed RNA polymerase subunit beta'
Decreases global transcription rate, 28% decreased growth rate, increased negative supercoiling level.
Glutathione import ATP-binding protein GsiA
For 3-hydroxyacyl-CoA dehydrogenase activity
Protein-L-isoaspartate O-methyltransferase
Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
Bacterial microcompartment shell protein PduK
Bifunctional adenosylcobalamin biosynthesis protein CobU
No activity; when associated with Ala-46.
No activity; when associated with Ala-47.
4-hydroxythreonine-4-phosphate dehydrogenase
Type I restriction enzyme StySJI methylase subunit
HTH-type DNA-binding transcriptional activator EutR
Shows thiamine-independent growth under anaerobic conditions, but thiamine dependent growth under aerobic conditions.
N-succinylglutamate 5-semialdehyde dehydrogenase
Probable transcriptional regulatory protein YebC
D-methionine transport system permease protein MetI
Thiol:disulfide interchange protein DsbD
Anaerobic nitric oxide reductase transcription regulator NorR
ECA polysaccharide chain length modulation protein
4-hydroxy-2-oxo-heptane-1,7-dioate aldolase
Almost loss of activity. Decreases helical content of the protein.
Strong decrease in activity. Small decrease in the helical content of the protein.
Bacterial microcompartment shell vertex protein EutN
Deoxyguanosinetriphosphate triphosphohydrolase
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
DNA protection during starvation protein
Pyrimidine-specific ribonucleoside hydrolase RihA
Oligopeptide transport ATP-binding protein OppD
Nucleophile; cysteine thiosulfonate intermediate
Phosphoadenosine 5'-phosphosulfate reductase
Lipopolysaccharide 1,2-glucosyltransferase
High-affinity branched-chain amino acid transport ATP-binding protein LivG
Loss of ubiquitin ligase activity. No thioester formation.
3-isopropylmalate dehydratase large subunit 1
Probable succinate transporter subunit YjjB
Probable 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase 1
Lysine/arginine/ornithine-binding periplasmic protein
Bacterial microcompartment shell protein PduA
Crystallized, central pore can be open or occluded, less permeable to substrate, cells grow slowly on 1,2-PD.
Crystallized, central pore is less symmetric, no change in shell permeability, increased permeability to glycerol, wild-type growth on 1,2-PD.
Crystallized, central pore is more hydrophobic but same size, less permeable to PD, 50% DDH activity, cells grow slowly on 1,2-PD.
Crystallized, central pore is occluded, less permeable to PD, 62% DDH activity, cells grow slowly on 1,2-PD.
Crystallized, central pore is occluded, less permeable to substrate, 75% DDH activity, cells grow slowly on 1,2-PD.
Decreased amounts of PduP in purified BMCs.
Improved growth on 1,2-PD, makes slightly larger BMCs, alters accumulation of PD metabolites.
No BMCs are made, forms hexamers which do not form arrays, dominant to wild-type protein. Little to no growth on 1,2-PD, cells elongate and are joined by filaments, BMC proteins aggregate near poles. No longer forms filaments upon overexpression, does not restore BMC formation to a double pduA-pduJ deletion. Does not form closed shells.
No change in shell permeability to PD, excretes more propionaldehyde, wild-type growth on 1,2-PD.
No change in shell permeability, wild-type growth on 1,2-PD.
Slow growth at limiting vitamin B12, wild-type at saturating conditions.
Subject to propionaldehyde toxicity, makes about 70% BMCs, protein shells appear wild-type but leak.
Subject to propionaldehyde toxicity, makes about 75% BMCs, shells are wrinkled and leaky.
Ribonucleoside-diphosphate reductase 2 subunit alpha
Potassium-transporting ATPase potassium-binding subunit
Charge relay system; for autoendoproteolytic cleavage activity
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
Putative 2,3-dihydroxypropane-1-sulfonate exporter
Voltage-gated ClC-type chloride channel ClcB
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Resistance to polyketide antibiotic thailandamide.
Cellulose synthase catalytic subunit [UDP-forming]
[Ribosomal protein S18]-alanine N-acetyltransferase
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Glutathione transport system permease protein GsiD
Bacterial microcompartment assembly protein PduM
Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
Maltose/maltodextrin import ATP-binding protein MalK
Contains three zinc binding sites, but the third site shows a decreased affinity for zinc.
Flagellar transcriptional regulator FlhC
4-hydroxy-tetrahydrodipicolinate synthase
L-Threonine dehydratase biosynthetic IlvA
Uncharacterized MFS-type transporter YhhS
DNA-directed RNA polymerase subunit omega
2-amino-3-ketobutyrate coenzyme A ligase
Oligopeptide transport system permease protein OppB
Reduces the ability to hydrolyze Asp-Leu without decreasing its ability to hydrolyze Leu-Leu.
50S ribosomal protein L3 glutamine methyltransferase
DNA-binding transcriptional regulator NtrC
Phosphoribosylformylglycinamidine cyclo-ligase
Vitamin B12 import system permease protein BtuC
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
Thiosulfate reductase cytochrome B subunit PhsC
Abolishes the dehydrogenase and ferrochelatase activities and reduces 10-fold the methyltransferase activity.
Abolishes the dehydrogenase and ferrochelatase activities and reduces 6-fold the methyltransferase activity.
Abolishes the methyltransferase activity and increases 3 and 4-fold the dehydrogenase and ferrochelatase activities, respectively.
Abolishes the methyltransferase activity and reduces 10 and 5-fold the dehydrogenase and ferrochelatase activities, respectively.
Abolishes the methyltransferase, dehydrogenase and ferrochelatase activities.
Putative 2-aminoethylphosphonate transport system permease protein PhnU
10-fold reduction in amidation activity with glutamine as substrate. The affinity for cobyrinate is nearly not affected whereas that for the c-monoamide intermediate decreases by 6-fold.
26-fold reduction in amidation activity with glutamine as substrate. The affinity for cobyrinate is nearly not affected whereas that for the c-monoamide intermediate decreases by 27-fold.
3-fold reduction in amidation activity with glutamine as substrate.
500-fold reduction in amidation activity with either glutamine or ammonia as substrate.
Cobyrinate a,c-diamide synthase, N-terminally processed
Loss of amidation activity when glutamine is used as substrate, and 750-fold reduction in amidation activity with ammonia as substrate.
Loss of amidation activity with either glutamine or ammonia as substrate.
Ribosomal protein S6--L-glutamate ligase
Phosphoglycerate transport system transcriptional regulatory protein PgtA