Putative acetolactate synthase large subunit IlvB2
Uncharacterized PPE family protein PPE51
Probable 1,4-alpha-glucan branching enzyme Rv3031
Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol pentamannoside mannosyltransferase
Uncharacterized ABC transporter ATP-binding protein Rv2326c
Probable glycerophosphodiester phosphodiesterase 1
Probable cation efflux system protein Rv2025c
80% loss of esterase and beta-lactamase activities.
Almost no change in esterase and beta-lactamase activities.
Loss of esterase and beta-lactamase activities.
Uncharacterized ABC transporter ATP-binding protein Rv0986
ATP-dependent Clp protease proteolytic subunit 1
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
Putative diacyglycerol O-acyltransferase Rv0895
Probable carboxylic ester hydrolase LipM
4-fold decrease in affinity for acylated mas protein. No change in catalytic activity.
40-fold decrease in affinity for acylated mas protein. No change in catalytic activity.
Less than 1.9-fold decrease in activity.
Phthiocerol/phthiodiolone dimycocerosyl transferase
Deoxyguanosinetriphosphate triphosphohydrolase-like protein
Only the chorismate mutase activity is observed.
Uncharacterized HTH-type transcriptional regulator Rv1816
DNA-binding protein HU homolog, propeptide removed
Uncharacterized glycosyltransferase Rv0539
Multidrug efflux system ATP-binding protein Rv1218c
Siderophore export accessory protein MmpS5
Acyl-ester intermediate; for acyltransferase activity
Acyl-thioester intermediate; for beta-ketoacyl synthase activity
Cannot form alpha-alkyl beta-ketoacids derivatives.
Protein no longer targeted to encapsulin nanocompartments, retains enzyme activity.
Abolishes phosphorylation by PknB. Strong decrease in affinity for MviN.
Decreases ability to bind to and regulate the activities of Gdh and GltB, but does not affect ability to inhibit Kgd.
Does not affect phosphorylation by PknG.
Fully oxidized recombinant protein tagged at both termini.
Fully reduced recombinant protein tagged at both termini.
Probable transcriptional regulator WhiB6
Uncharacterized metal-dependent hydrolase TatD
Uncharacterized PE-PGRS family protein PE_PGRS46
3-oxoacyl-[acyl-carrier-protein] reductase MabA
70% decrease in activity with acetoacetyl-CoA as substrate. Does not affect NADP binding.
Complete protein inactivation and freezes the catalytic site into its closed form. Totally inactive; when associated with V-60 and L-144.
Displays a lower activity than the wild-type and a slightly decreased affinity for the cofactor. Retains 84% of activity; when associated with L-144. Totally inactive; when associated with A-139 and L-144.
Loss of activity. Impaired NADPH binding.
Phosphomimetic mutant that retains less than 10% of wild-type reductase activity. Impaired NADPH binding. Overproduction of the mutant leads to a significant inhibition of de novo biosynthesis of mycolic acids.
Recombinant protein expressed in E.coli.
Retains 22% of wild-type reductase activity. Strong decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-114.
Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-114 and A-191.
Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-191.
Stabilizes the catalytic loop in its open active form. Retains 84% of activity; when associated with V-60. Totally inactive; when associated with V-60 and A-139.
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Chromosomal replication initiator protein DnaA
O-(3'-phospho-DNA)-tyrosine intermediate
Phosphate import ATP-binding protein PstB 1
Ribosomal RNA small subunit methyltransferase E
Cytochrome bc1 complex cytochrome c subunit
Abolishes EsxB-EsxA heterodimer interaction with EccCb1 and EspA, maintains interaction with EccA2 and EccE2.
Abolishes EsxB-EsxA heterodimer interaction with EccCb1, maintains interaction with EspA, EccA2 and EccE2.
Abolishes EsxB-EsxA heterodimer interaction with EccCb1, weaker interaction with EspA, maintains interaction with EccA2 and EccE2.
Abolishes interaction with EccCb1, but not with EsxA.
Abolishes interaction with EccCb1, but not with EsxA. No change in stability, but loss of secretion.
2-heptyl-1-hydroxyquinolin-4(1H)-one methyltransferase
6.7-fold increase in catalytic efficiency with componud 14 as substrate.
Uncharacterized PPE family protein PPE59
Trehalose import ATP-binding protein SugC
2'-acyl-2-O-sulfo-trehalose (hydroxy)phthioceranyltransferase PapA1
Molybdenum import ATP-binding protein ModC
Decrease in FAD-binding and loss of activity.
Mycobactin import ATP-binding/permease protein IrtA
No change in FAD-binding and in activity.
Vitamin B12-dependent ribonucleoside-diphosphate reductase
Complete loss of acetyl-coenzyme A synthetase activity.
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B
Resolvase/invertase-type recombinase catalytic
N(G),N(G)-dimethylarginine dimethylaminohydrolase
17-fold increased resistance to fluoroquinolones, 4- to 8-fold reduction in fluoroquinolone-induced DNA cleavage.
25- 45-fold increased resistance to fluoroquinolones, 4- to 8-fold reduction in fluoroquinolone-induced DNA cleavage. Supercoiling, relaxation, decatenation activities no longer inhibited by MfpA.
4- to 16-fold more efficient in fluoroquinolone-induced DNA cleavage alone. Hypersusceptibile, 2- to 14-fold higher sensitivity to fluoroquinolones, 2- to 8-fold more efficient in fluoroquinolone-induced DNA cleavage; when associated with A-80.
80-fold increased resistance to fluoroquinolones, 32- to 64-fold reduction in fluoroquinolone-induced DNA cleavage.
Confers ciprofloxacin resistance, in clinical isolate.
Confers fluoroquinolone resistance, IC(50) is 2- to 26-fold higher than wild-type.
Confers fluoroquinolone resistance, IC(50) is 3- to 43-fold higher than wild-type, in strains H37Ra and H37Rv.
Increased susceptibility to fluoroquinolones (makes sequence more like E.coli), supercoiling, relaxation, decatenation activities still inhibited by MfpA.
No change in supercoiling activity, wild-type decatenation or fluoroquinolone-induced DNA cleavage.
No supercoiling activity, almost wild-type decatenation activity, wild-type fluoroquinolone-induced DNA cleavage.
No supercoiling activity, wild-type decatenation and fluoroquinolone-induced DNA cleavage.
No supercoiling or decatenation activity, decreased fluoroquinolone-induced DNA cleavage.
O-(5'-phospho-DNA)-tyrosine intermediate
Reduced supercoiling activity, wild-type decatenation and fluoroquinolone-induced DNA cleavage.
Significant reduction in DNA wrapping and supercoiling activity, no change in decatanation or relaxation activities.
Slight reduction in supercoiling activity.
Slight resistance to fluoroquinolones. Hypersusceptibile, 2- to 14-fold higher sensitivity to fluoroquinolones, 2- to 8-fold more efficient in fluoroquinolone-induced DNA cleavage; when associated with G-90.
Wild-type decatenase activity (changes residue to match E.coli).
Wild-type decatenase activity (changes residues to match E.coli).
Wild-type supercoiling, decatenation and fluoroquinolone-induced DNA cleavage.
Uncharacterized glycosyl hydrolase Rv3401
Loss of protease activity. Shows neither autoproteolytic nor proteolytic activity against beta-casein.
Ribosomal RNA small subunit methyltransferase H
Hydrophilic compounds import ATP-binding/permease protein BacA
Loss of cobalamin transport. Shows background ATPase activity.
No longer neutralizes the toxic effect of MbcT.
Carbamoyl-phosphate synthase small chain
Can rescue the moenomycin sensitivity phenotype and the aberrant short-length phenotype of the deletion mutant.
Can rescue the moenomycin sensitivity phenotype of the deletion mutant, but cannot rescue the aberrant short-length phenotype.
DNA-directed RNA polymerase subunit beta'
Cholesterol ring-cleaving hydrolase IpdA subunit
Probable ATP-dependent helicase DinG homolog
Electron transfer flavoprotein subunit alpha
Uncharacterized MFS-type transporter Rv2994
Affects protein stability. Decreases affinity for DNA and dimerization capacity.
Affects protein stability. Decreases affinity for DNA. Does not affect dimerization.
Decreases affinity for DNA. Does not affect dimerization.
Moderate impact on protein stability. Does not affect DNA binding.
Copper-sensing transcriptional repressor RicR
Cannot complement the growth defect of the disruption mutant. Cannot cleave GroEL2. Is unable to hydrolyze azocasein.
Small decrease in activity with azocasein as substrate. Almost abolishes cleavage of GroEL2.
Glucose-1-phosphate thymidylyltransferase
3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase beta subunit
Loss of activity. Still forms heterotetramers.
Retains 2-4% of activity. Still forms heterotetramers.
Does not affect formation of the PE25/PPE41 dimer, but abolishes EspG5 binding to PE25/PPE41.
Does not disrupt the interaction with EspG5.
Dihydrolipoyllysine-residue acyltransferase component of branched-chain alpha-ketoacid dehydrogenase complex
Uncharacterized PPE family protein PPE63
Uncharacterized response regulatory protein Rv3143
Putative 3-methyladenine DNA glycosylase
Long-chain-fatty-acid--CoA ligase FadD15
Probable fatty acid methyltransferase Rv3720
Uncharacterized N-acetyltransferase Rv2669
Uncharacterized HTH-type transcriptional regulator Rv0377
Probable mycobacterial cidal antitoxin Rv3188
Gamma-glutamyl-hercynylcysteine sulfoxide hydrolase
Mycofactocin precursor peptide peptidase
Loss of resuscitating activity on M.luteus.
2-fold increase in ATPase activity (residues 72-448 only).
6-fold increase in ATPase activity (residues 106-463 only).
No ATPase activity (residues 106-480 only).
Retains 40% ATPase activity (in residues 72-480).
Retains only 20% decrease ATPase activity (in residues 72-480); probably due to loss of secondary structure.
No interaction with PbpB (PBP3, FtsI). Slower growth, increased sensitivity to H(2)O(2) and to antibiotics ofloxacin and isoniazid, increased degradation of PbpB. Decreased survival in human macrophage line THP-1.
Strong decrease in phosphorylation of Wag31.
Uncharacterized RNA pseudouridine synthase Rv1540
Loss of growth, no ATPase activity. No DNA unwinding. Does not translocate along DNA.
Not essential for growth, no helicase activity. Wild-type ATPase activity, translocates along DNA. No DNA unwinding.
Interaction with polymerase core subunit RpoC
Putative asparagine synthetase [glutamine-hydrolyzing]
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv1896c
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Does not bind DNA and does not form dimers.
Does not bind DNA, but retains the capacity to form dimers; when associated with G-32.
Does not bind DNA, but retains the capacity to form dimers; when associated with G-35.
HTH-type transcriptional regulator Rv2034
Probable ribonucleotide transport ATP-binding protein mkl
Probable cation-transporting P-type ATPase J
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Uncharacterized protein Rv2386A/RVBD_2386A
Probable S-adenosylmethionine-dependent methyltransferase Rv3030
The binding mode of azoles and some arylamines are reverted from type II to type I because of hydrophobic and steric interactions with the alanine side chain.
No large change in protein assembly or structure, decreased thermostability, 3.5-fold reduction in oxidation of Fe(2+), 20% reduction in Fe(3+) release rate.
Protein no longer targeted to encapsulin nanocompartments.
Nearly abolishes DHNA-CoA synthase activity.
Reduces affinity for substrate. Nearly abolishes DHNA-CoA synthase activity.
Uncharacterized AAA domain-containing protein Rv2559c
Increases KdpD induction at 0 and 250 uM K(+).
Medium/long-chain-fatty-acid--CoA ligase FadD6
Trehalose-2-sulfate acyltransferase PapA2
700-fold decrease in the rate of the first half-reaction using OPS. Affects neither the rate of the first half-reaction using OAS nor the rate of the second half-reaction using sulfide or CysO-COSH.
Decreased lifetime of the alpha-aminoacrylate reaction intermediate, increased susceptibility to oxidation by oxidative agents such as hydrogen peroxide, and partial loss of selectivity towards CysO-COSH as sulfur donor.
Putative uncharacterized protein Rv0095c
Uncharacterized PPE family protein PPE69
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0726c
Putative succinate-semialdehyde dehydrogenase [NADP(+)] 2
Uncharacterized HTH-type transcriptional regulator Rv2250c
Probable transcriptional regulatory protein TcrX
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
Cysteine sulfenic acid (-SOH) intermediate
Putative acetolactate synthase small subunit
Putative envelope-preserving system protein Rv2743c
Uncharacterized membrane protein Rv1735c
Severely affects the affinity toward GTP and causes a significant decrease in enzyme activity.
Signal recognition particle receptor FtsY
2.1-fold decrease in KM for ADPR. 1940-fold decrease in kcat with ADPR as substrate.
2.3-fold decrease in KM for ADPR. 1150-fold decrease in kcat with ADPR as substrate.
2.7-fold decrease in KM for ADPR. 300-fold decrease in kcat with ADPR as substrate.
3-fold decrease in KM for ADPR. 910-fold decrease in kcat with ADPR as substrate.
Multidrug efflux system permease protein Rv1217c
About 50% succinylation in vitro, reduces specific activity 7-fold.
Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
Very low levels of succinylation in vitro, reduces specific activity 15-fold.
Decreases structure stability of OmpA-like domain.
Increases conformational stability of OmpA-like domain. Does not alter peptidoglycan-binding.
Loss of peptidoglycan-binding; in association with A236.
Trehalose transport system permease protein SugB
Phenolphthiocerol/phthiocerol polyketide synthase subunit A
Uncharacterized NAD-dependent oxidoreductase Rv0687
Uncharacterized MFS-type transporter Rv1877
Uncharacterized aminotransferase Rv2231c
Abolishes interaction with host ubiquitin, impairs host xenophagy and increases bacterial loads in mice with enhanced inflammatory responses.
Reduces interaction with host ubiquitin.
Reduces interaction with host ubiquitin. Shows enhanced viability, reduced p62 aggregation and reduced colocalization with LC3 and LAMP1 in BMDMs.
Malto-oligosyltrehalose trehalohydrolase
(5R,7aS)-5-hydroxy-7a-methyl-1-oxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA reductase
Phosphoribosylformylglycinamidine synthase subunit PurQ
Putative diacyglycerol O-acyltransferase Rv3740c
Multifunctional 2-oxoglutarate metabolism enzyme
Proton acceptor; for succinyltransferase activity
Long-chain-fatty-acid--AMP ligase FadD28
No activity with lauric acid (C12 fatty acid) as substrate. Instead, it catalyzes activation of acetic acid (C2 fatty acid) as acetyl-AMP.
Shows substantial decrease (69%) in the activity with lauric acid (C12 fatty acid) as substrate but shows a 4-fold higher activity with the long chain fatty acyl capric acid (C10 fatty acid) as substrate.
Uncharacterized tRNA/rRNA methyltransferase Rv0881
Probable dolichyl-phosphate-mannose--protein mannosyltransferase
Long-chain fatty acyl-CoA thioesterase FcoT
3-ketosteroid-9-alpha-monooxygenase, oxygenase component
Probable polyglutamine synthesis accessory protein Rv0574c
Putative diacyglycerol O-acyltransferase Rv1760
Flavin-dependent monooxygenase, oxygenase subunit HsaA
Putative pterin-4-alpha-carbinolamine dehydratase
Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase
Probable low-affinity inorganic phosphate transporter
Decreases resistance to ethidium bromide, tested in M.smegmatis.
Probable triacylglyceride transporter Rv1410c
Susceptible to ethidium bromide, tested in M.smegmatis.
ESX-2 secretion-associated protein EspG2
17-hydroxy-3-oxo-4-pregnene-20-carboxyl-CoA lyase
L-threonine dehydratase biosynthetic IlvA
Abolishes DNA-binding and repressor activity; when associated with A-52 and A-57.
Abolishes DNA-binding and repressor activity; when associated with A-52 and A-62.
Abolishes DNA-binding and repressor activity; when associated with A-57 and A-62.
HTH-type transcriptional repressor Rv3405c
Uncharacterized S-adenosylmethionine-dependent methyltransferase Rv3037c
Cannot bind DNA; when associated with A-45 and A-50.
Cannot bind DNA; when associated with A-45 and A-55.
Cannot bind DNA; when associated with A-50 and A-55.
HTH-type transcriptional repressor Rv1474c
Phosphate transport system permease protein PstA 2
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
S-adenosylmethionine-dependent methyltransferase Rv2258c
Bifunctional apolipoprotein N-acyltransferase/polyprenol monophosphomannose synthase
Abolishes ATP-binding and diadenylate cyclase activity.
Uncharacterized PPE family protein PPE33
Phosphatidyl-myo-inositol mannosyltransferase
Abolished formylglycine-generating enzyme activity.
Strongly reduced formylglycine-generating enzyme activity.
4-fold decrease in affinity relative to the wild-type and able to synthesize prenyl diphosphates containing 20 and 50 carbon atoms when GPP and EE-FPP are used as the allylic substrates, respectively, whereas the wild-type enzyme generates product that is restricted to 15 carbon atoms with GPP as substrate and does not react with EE-FPP; when associated with G-107.
No detectable activity. 4-fold decrease in affinity relative to the wild-type and able to synthesize prenyl diphosphates containing 20 and 50 carbon atoms when GPP and EE-FPP are used as the allylic substrates, respectively, whereas the wild-type enzyme generates product that is restricted to 15 carbon atoms with GPP as substrate and does not react with EE-FPP; when associated with A-84.
Uncharacterized PPE family protein PPE20
D-glycero-alpha-D-manno-heptose 7-phosphate kinase
Probable diacyglycerol O-acyltransferase tgs3
No effect on activity. 10-fold more resistant to inhibition by Li(+).
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
ATP-dependent Clp protease proteolytic subunit 2
Uncharacterized PPE family protein PPE42
Uncharacterized ABC transporter ATP-binding protein Rv1273c
N5-carboxyaminoimidazole ribonucleotide synthase
Probable nicotinate-nucleotide adenylyltransferase
DNA-directed RNA polymerase subunit omega
Putative ATP-binding protein Rv3427c in insertion sequence
No phosphorylation by EnvZ kinase in vitro, does not bind DNA. Poor growth in infected macrophages and upon mouse infection. Fewer bacteria are mistargeted to lysosomes.
No phosphorylation by EnvZ kinase in vitro. Binds DNA in the absence but not presence of EnvZ and ATP. A merodiploid strain has late growth defects. May mimic the phosphorylated state.
No phosphorylation by EnvZ kinase in vitro. Binds fbpB and oriC DNA in the presence and absence of EnvZ and ATP. A merodiploid strain has late growth defects, fbpB is down-regulated. May be constitutively active.
Phosphorylated by EnvZ kinase in vitro, reverses many phenotypes of mtrB knockout.
Putative peptide transport permease protein Rv1283c
Broad-specificity linear acyl-CoA dehydrogenase FadE5
Decreases affinity for eicosanoyl-CoA; when associated with A-447.
Increases affinity for eicosanoyl-CoA; when associated with A-447.
Putative diacyglycerol O-acyltransferase Rv3371
Decrease in substrate-binding and in catalytic efficiency.
Does not affect substrate-binding, but decreases the catalytic efficiency and the Vmax.
Does not distord the binding site architecture. No change in catalytic efficiency and Vmax.
Large increase in the Km and strong decrease in catalytic efficiency. Loses the ability to self-associate in the presence of ATP and the cooperativity is nearly abolished.
S-adenosylmethionine-dependent methyltransferase UmaA
Probable doxorubicin resistance ABC transporter permease protein DrrC
Proton donor/acceptor; for phosphatase activity
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
Trk system potassium uptake protein TrkA
TVP38/TMEM64 family membrane protein Rv0625c
Abolishes pupylation and depupylase activity.
Loss of catalytic activity but still able to mediate uptake of glucose and phosphocholine; when associated with N-353.
Loss of catalytic activity but still able to mediate uptake of glucose and phosphocholine; when associated with N-481.
200000-fold decrease in catalytic efficiency with cefamandole as substrate.
Loss of catalytic activity with cefamandole as substrate.
No longer exported via the Tat-system. Loss of cell resistance to beta-lactam antibiotics.
Only minor impairment of catalytic activity with both nitrocefin and ampicillin. Still inhibited by clavulanate and carbapenems.
Significant increase in ampicillin resistance. 2-fold and 3-fold increase in catalytic efficiency with ampicillin and nitrocefin as substrate, respectively, mainly due to an increase in substrate affinity.
Significant reduction of catalytic activity for both nitrocefin and ampicillin. Leads to in vitro clavulanate resistance and decreased susceptibility to carbapenem inhibitors, but is still susceptible to ampicillin-clavulanate in vivo.
Significant reduction of catalytic activity for both nitrocefin and ampicillin. Only minor impairment of the inactivation by clavulanate. Larger increase in resistance to carbapenems.
Flavin-dependent monooxygenase, reductase subunit HsaB
Type 1 encapsulin shell protein, N-terminally processed
Probable chromosome-partitioning protein ParB
Uncharacterized PE-PGRS family protein PE_PGRS54
Impairs interaction with PknB and PapA5.
Impairs interaction with PknB. Does not affect interaction with PapA5.
p-hydroxybenzoic acid--AMP ligase FadD22
Completely inactive mutant. The bifurcated hydrogen bond between D-270 and the ribose of NAD is replaced by a single hydrogen bond.
Uncharacterized PPE family protein PPE50
Peptide methionine sulfoxide reductase MsrA
Phosphate transport system permease protein PstA 1
PGL/p-HBAD biosynthesis glycosyltransferase Rv2958c
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase
Uncharacterized HTH-type transcriptional regulator Rv1255c
Probable trans-aconitate 2-methyltransferase
N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Decreases affinity for NaHCO(3) 3-fold, for CTP 1.8-fold and for GTP 3.8-fold.
Alpha-pyrone synthesis polyketide synthase-like Pks18
Cannot be recovered in the soluble form.
Completely abolishes the polyketide synthase activity with lauroyl-CoA and palmitoyl-CoA.
Efficiently catalyzed the synthesis of the triketide pyrone of the C6 starter unit and shows weak polyketide synthase activity with the C12 starter molecule.
Increase in the synthesis of the tetraketide products.
No significant change in polyketide synthase activity.
Retained reasonable polyketide synthase activity with lauroyl-CoA but cannot use palmitoyl-CoA.
Unable to synthesize any polyketide products.
Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase beta1 subunit
Still interacts with EsxG, but impairs interaction with host HGS/HRS; when associated with A-76.
Still interacts with EsxG, but impairs interaction with host HGS/HRS; when associated with A-77.
Dihydrofolate synthase/folylpolyglutamate synthase
In a spontaneous PAS-resistant mutant strain.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility, confirmimg that this mutation confers high level resistance to PAS.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility.
In a spontaneous PAS-resistant mutant strain; complementation with wild-type folC restores PAS susceptibility; 5-fold reduction in DHFS activity relative to wild-type; loss of the ability to glutaminate H2PtePAS.
In clinical isolate: Q36; PAS-resistant; complementation with wild-type folC restores PAS susceptibility.
In clinical isolates: Q274 and 501063; PAS-resistant; complementation with wild-type folC restores PAS susceptibility.
In clinical isolates: Q449 and 1314; PAS-resistant; complementation with wild-type folC restores PAS susceptibility; 7-fold reduction in DHFS activity relative to wild-type; loss of the ability to glutaminate H2PtePAS.
Uncharacterized PPE family protein PPE46
Lack of phosphorylation by PknF; when associated with A-25.
Lack of phosphorylation by PknF; when associated with A-54.
Probable methylmalonyl-CoA mutase large subunit
Nucleophile; methyl group acceptor from methylphosphotriester
Probable bifunctional transcriptional activator/DNA repair enzyme AlkA
Proton acceptor; for DNA glycosylase activity
Uncharacterized HTH-type transcriptional regulator Rv3095
Markedly increases peptidolytic activity. Disappearance of the apparent obstruction in alpha rings. Designated open-gate mutant.
Putative thiosulfate sulfurtransferase SseA
Long-chain-fatty-acid--AMP ligase FadD26
Uncharacterized HTH-type transcriptional regulator Rv0474
Transposase for insertion sequence element IS1081
Common variant in the Beijing-W clade/SCG-2 phylogenetic group.
ATP-dependent Clp protease ATP-binding subunit ClpX
2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1.
4-hydroxy-tetrahydrodipicolinate reductase
Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Uncharacterized PPE family protein PPE21
CRP-like cAMP-activated global transcriptional regulator
No effect on activity. Decreases Km for NADP 2-fold.
Decaprenylphosphoryl-2-keto-beta-D-erythro-pentose reductase
DNA-directed RNA polymerase subunit beta
Does not affect association with TRCF, but weakens interaction with CarD.
In strain: vr11 and RJ37; rifampicin-resistant.
In strain: vr12 and SP22; rifampicin-resistant.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Uncharacterized PPE family protein PPE14
Probable copper-exporting P-type ATPase V
Phosphate import ATP-binding protein PstB 2
Abolishes proteasome substrate degradation and protection against RNI.
Abolishes proteasome substrate degradation.
Does not dramatically affect proteasome substrate degradation.
Impairs Mpa hexamerization; when associated with A-187 and E-235.
Impairs Mpa hexamerization; when associated with E-173 and A-187.
Impairs Mpa hexamerization; when associated with E-173 and E-235.
Reduces both ATPase activity and ATP affinity. Abolishes proteasome substrate degradation and protection against RNI.
Retains ATPase and unfolding activities, yet abolishes proteasome substrate degradation and protection against RNI. Is also highly attenuated in mice.
Severely reduces ATPase activity. Abolishes proteasome substrate degradation and protection against RNI.
Severely reduces ATPase activity. Abolishes protection against RNI.
Imidazole glycerol phosphate synthase subunit HisH
Polyribonucleotide nucleotidyltransferase
Nicotinate phosphoribosyltransferase pncB2
Probable transport accessory protein MmpS2
13% supercoiling, 10% decatenation, 42% DNA cleavage in presence of norfloxacin.
15% supercoiling, 19% decatenation, 143% DNA cleavage in presence of norfloxacin.
17-fold increased resistance to fluoroquinolones, slightly increased DNA cleavage in absence of drugs.
18% supercoiling, 11% decatenation, 131% DNA cleavage in presence of norfloxacin.
37% supercoiling, 54% decatenation, 126% DNA cleavage in presence of norfloxacin.
<3% supercoiling, 18% decatenation, 75% DNA cleavage in presence of norfloxacin.
Increased resistance to aminopyrazinamides, however genome not sequenced.
Increased resistance to pyrrolamides and novobiocin, however genome not sequenced.
Increased susceptibility to fluoroquinolones, half supercoiling activity, no fluoroquinolone-induced DNA cleavage (makes sequence more like E.coli).
Uncharacterized PPE family protein PPE22
Putative decaprenylphosphoryl-5-phosphoribose phosphatase Rv3807c
2-fold increase in bis(pNPP) hydrolysis. 4-fold decrease in pNPP hydrolysis. 5-fold decrease in 2',3'-cAMP hydrolysis.
25% decrease in bis(pNPP) hydrolysis. 70% decrease in cAMP hydrolysis. Decreases interaction with the cell wall.
40% decrease in bis(pNPP) hydrolysis. 80% decrease in 3',5'-cAMP and 2',3'-cAMP hydrolysis.
40% decrease in bis(pNPP) hydrolysis. Strong decrease in 3',5'-cAMP and 2',3'-cAMP hydrolysis. Abolishes pNPP hydrolysis. Decreases interaction with the cell wall.
50% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Decreases interaction with the cell wall.
50% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Strong decrease in interaction with the cell wall.
75% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Decreases catalytic efficiency and shows lower affinity for both linear and cyclic substrates. Almost completely abrogates interaction with the cell wall in vitro.
8-fold decrease in phosphorylation. Affects localization to the cell wall.
80% decrease in hydrolysis of 3',5'-cAMP and bis(pNPP). 40% decrease in 2',3'-cAMP hydrolysis. Strong decrease in interaction with the cell wall.
90% decrease in bis(pNPP) hydrolysis. Does not affect cAMP hydrolysis. Strong decrease in interaction with the cell wall.
Does not affect localization to the cell wall.
Loss of bis(pNPP) and 3',5'-cAMP hydrolysis. Strong decrease in 2',3'-cAMP hydrolysis. Slight decrease in interaction with the cell wall.
cAMP/cGMP dual specificity phosphodiesterase Rv0805
Siderophore export accessory protein MmpS4
Almost loss of activity. Shows very poor binding to ATP.
Decreases Km for both ATP and dephospho-CoA. Has minimal effect on kcat.
Does not affect Km for ATP, but shows a 15-fold increase in the Km for dephospho-CoA. Decrease in kcat and strong decrease in catalytic efficiency.
Does not affect Km for ATP, but shows a 2.5-fold increase in the Km for dephospho-CoA.
Does not change either the Km or the kcat of the reaction considerably.
Increases Km for both ATP and dephospho-CoA, but increases the enzymatic turnover.
Shows a 50% increase in the Km for ATP and a 19% reduction in kcat.
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
2-fold decrease in kcat for 4-methylumbelliferyl heptanoate and loss of phopholipase A activity; when associated with C-132.
2-fold decrease in kcat for 4-methylumbelliferyl heptanoate and loss of phopholipase A activity; when associated with C-288.
Loss of activity for both short- and long-chain substrates.
50% reduction in oxidation activity of dithiothreitol and 60% reduction in oxidation of thiocyanate.
50% reduction in oxidation activity of dithiothreitol and thiocyanate.
In reconstituted in vitro system retains no enzyme activity.
Interchain (with C-133 in AhpD); transient
Retains about 10% activity with tert-butylhydroperoxide. In reconstituted in vitro system retains 30% enzyme activity.
Very poor oxidation activity of dithiothreitol and thiocyanate.
3-oxoacyl-[acyl-carrier-protein] synthase 2
Decreases phosphorylation. Lack of phosphorylation; when associated with A-313.
Decreases phosphorylation. Lack of phosphorylation; when associated with A-315.
Phosphomimetic mutant. Loss of acid-fast staining and reduced pathogenicity; when associated with D-313.
Phosphomimetic mutant. Loss of acid-fast staining and reduced pathogenicity; when associated with D-315.
D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Abolishes autophosphorylation as well as dephosphorylation of phospho-MprA.
Signal transduction histidine-protein kinase/phosphatase MprB
Release factor glutamine methyltransferase
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Probable diacyglycerol O-acyltransferase tgs1
Helical; Signal-anchor for type II membrane protein
N5-carboxyaminoimidazole ribonucleotide mutase
Transcription termination/antitermination protein NusG
Decrease in autophosphorylation activity. Does not affect transphosphorylation activity.
Does not affect autophosphorylation and transphosphorylation activities.
Strong decrease in autophosphorylation activity. Lack of transphosphorylation activity.
dTDP-4-amino-4,6-dideoxyglucose formyltransferase
Uncharacterized HIT-like protein Rv1262c
Biotin-dependent acyl-coenzyme A carboxylase alpha3 subunit
1500-fold decrease in catalytic activity while no effect on lipid substrate affinity.
24-fold decrease in catalytic activity while no effect on lipid substrate affinity.
Confers INH and ETH resistance. The mutant is 17 times more resistant to inhibition by the INH-NAD adduct. 5- to 6-fold decrease in affinity for NADH that results from a perturbation in the hydrogen-bonding network that stabilizes NADH binding. Nearly no effect on the velocity of the enzyme. Has no impact on the susceptibility to pyridomycin.
Confers pyridomycin resistance. Has no impact on the susceptibility to isoniazid and moxifloxacin. 14-fold decrease in NADH affinity, while no effect on catalytic activity.
Enoyl-[acyl-carrier-protein] reductase [NADH]
No effect on catalytic activity. Loss of phosphorylation. Does not alter growth of M.tuberculosis.
No effect on the enzyme's catalytic ability or on its ability to bind NADH.
Severely impairs catalytic activity, as a consequence of a reduced binding affinity to NADH. These single point mutations are lethal to M.tuberculosis. These mutants fail to complement growth and mycolic acid defects of an inhA-thermosensitive M.smegmatis strain, in a similar manner to what is observed following isoniazid treatment.
Putative acetolactate synthase large subunit IlvX
Uncharacterized HTH-type transcriptional regulator Rv2282c
Alanine and proline-rich secreted protein Apa
Chemically or enzymatically deglycosylated protein.
No change in activity. Does not affect regulation by metal ions.
No change in activity. Lack of regulation by metal ions.
Succinate--CoA ligase [ADP-forming] subunit alpha
Fatty acid ABC transporter ATP-binding/permease protein
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Probable peptidoglycan glycosyltransferase FtsW
Uncharacterized PPE family protein PPE12
Uncharacterized glycosyl hydrolase Rv2006
Crossover junction endodeoxyribonuclease RuvC
Confers resistance to LPZS. Remains susceptible to IPA.
Confers resistance to Q203 and IPA. Remains susceptible to LPZS.
Cytochrome bc1 complex cytochrome b subunit
2200-fold decrease in catalytic efficiency with coenzyme A.
6600-fold decrease in catalytic efficiency with coenzyme A.
Propanal dehydrogenase (CoA-propanoylating)
Putative peptide transport permease protein Rv1282c
Doxorubicin resistance ABC transporter permease protein DrrB
Biotin-dependent long chain acyl-coenzyme A carboxylase beta4 subunit
PaaX domain-containing protein, C-domain protein
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0146
10-fold decrease in the specificity constant for thiosulfate compared to wild-type enzyme but little change in the specificity constant for the substrate OPS.
Lack of activity. Confers resistance to TP053.
Putative inactive phenolphthiocerol synthesis polyketide synthase type I Pks15
Uncharacterized HTH-type transcriptional regulator Rv0196
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
Maximal improvement in solubility; when associated with M-47 and K-69.
Maximal improvement in solubility; when associated with T-6 and K-69.
Maximal improvement in solubility; when associated with T-6 and M-47.
Loss of PA-824 and menadione reductase activity.
Acyl-ester intermediate; for transpeptidase activity
Proton donor; for transglycosylase activity
Shows less than 0.1% of wild-type activity with peptides substrates.
Shows less than 1% of wild-type activity.
Shows less than 1% of wild-type activity. Does not affect macrophage apoptosis.
Shows less than 10% of the wild-type activity towards phosphotyrosine peptides, but remains unaltered towards pNPP and phosphoserine/threonine peptides.
Shows new activity for di-phosphorylated substrates PI(3,4)P2, PI(4,5)P2 and PI(3,4,5)P3.
Triple specificity protein phosphatase PtpB
Uncharacterized GMC-type oxidoreductase Rv0492c
Large-conductance mechanosensitive channel
Acyl-[acyl-carrier-protein] dehydrogenase MbtN
Loss of ATPase and DNA unwinding, partially inhibits DNA strand exchange.
Leads to the production of longer chain product, specifically (E,E)-farnesyl diphosphate (FPP), in addition to GPP.
Probable cobalt/nickel-exporting P-type ATPase
Able to bind DNA, no longer induces hypoxic response.
DNA-binding transcriptional activator DevR/DosR
No longer induces expression of DevR regulon under hypoxic conditions, phosphorylated slowly and incompletely by DevS, decreased cooperative binding of target DNA.
No phosphorylation by DevS (DosS) nor by DosT.
Energy-dependent translational throttle protein EttA
PGL/p-HBAD biosynthesis glycosyltransferase Rv2957
Acyl-ester intermediate; for acyltransferase 1 activity
Acyl-ester intermediate; for acyltransferase 2 activity
Acyl-thioester intermediate; for beta-ketoacyl synthase 1 activity
Acyl-thioester intermediate; for beta-ketoacyl synthase 2 activity
HTH-type transcriptional repressor KstR2
Uncharacterized PE-PGRS family protein PE_PGRS44
Uncharacterized PPE family protein PPE30
Potassium-transporting ATPase potassium-binding subunit
Phenylalanine--tRNA ligase alpha subunit
Uncharacterized HTH-type transcriptional regulator Rv0023
Uncharacterized HTH-type transcriptional regulator Rv0880
Putative mycofactocin biosynthesis transcriptional regulator MftR
Probable fatty acyl-CoA transferase Rv3272
Uncharacterized MFS-type transporter EfpA
Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit
Expressed in M.bovis, lipidated and glycosylated.
No longer increases KdpD induction at 0 and 250 uM K(+); when associated with S-185 and V-197.
No longer increases KdpD induction at 0 and 250 uM K(+); when associated with T-132 and S-185.
No longer increases KdpD induction at 0 and 250 uM K(+); when associated with T-132 and V-197.
Putative diacylated glycolipid transporter LprF
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA
Probable methylmalonyl-CoA mutase small subunit
Decreased interaction with Wag31. Loss of interaction, reduced protection from Rip1 proteolysis; when associated with 427-H--V-429 deletion.
Decreased interaction with Wag31. Loss of interaction, reduced protection from Rip1 proteolysis; when associated with 488-Q--S-490 deletion.
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
Putative HTH-type transcriptional regulator Rv0890c
Considerable effects on the heme macrocycle conformation. Mutant leads to a more planar heme conformation.
Has little effect on the heme conformation but significantly alters the environment of the heme and the affinity for azoles.
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, propeptide removed
Putative glutamine amidotransferase Rv2859c
Putative conjugal transfer protein Rv3659c
550-fold decrease in catalytic activity.
9000-fold decrease in catalytic activity.
10-fold reduction in transphosphorylation activity. This mutant strain shows severely compromised growth. Loss of autophosphorylation activity and weak transphosphorylation activity; when associated with A-174.
4-fold reduction in transphosphorylation activity.
5-fold reduction in transphosphorylation activity. Loss of autophosphorylation activity and weak transphosphorylation activity; when associated with A-172.
Loss of auto- and transphosphorylation activity.
Loss of autophosphorylation activity. This mutation results in cell death.
Abolishes phosphorylation. Does not affect activity.
Mimics constitutive phosphorylation. Exhibits markedly decreased transacylation, malonyl-AcpM decarboxylation and condensing activities.
Mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III
Retains 21% of wild-type activity. Loss of activity; when associated with A-161.
Retains 69% of wild-type activity. Loss of activity; when associated with A-42 or A-46.
Retains 7% of wild-type activity. Loss of activity; when associated with A-161.
Uncharacterized HTH-type transcriptional regulator Rv0494
Lack of phosphorylation. Retains DNA-binding activity.
Fluoroquinolones export ATP-binding protein Rv2688c
Lack of phosphorylation. Increases the growth of mycobacteria.
Probable CDP-diacylglycerol pyrophosphatase
Nucleoside triphosphate pyrophosphohydrolase
Pyrophosphohydrolase activity is reduced 20-fold. It affects the magnesium binding and the protein structure.
No effect. No longer neutralizes VapC4 in full-length VapB4; when associated with G-48; R-48 or E-54.
No effect. No longer neutralizes VapC4 in full-length VapB4; when associated with low levels of G-48.
No effect. No longer neutralizes VapC4 in full-length antitoxin, decreased interaction with VapC4 (tested for G-48 only); when associated with A-64.
No effect. No longer neutralizes VapC4 in full-length antitoxin; when associated with A-64.
No longer neutralizes VapC4 in full-length VapB4; when associated with low levels of G-48.
No longer toxic, does not degrade NAD(+).
Succinate dehydrogenase 2 membrane subunit SdhC
D-alanyl-D-alanine carboxypeptidase DacB2
In strain: 9106, 9181, An03, F12, F18 and TCVGH21; resistant to streptomycin.
In strain: C37; probably resistant to streptomycin.
In strain: F05; resistant to streptomycin.
In strain: F18; resistant to streptomycin.
In strain: TCVGH25; probably resistant to streptomycin.
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Abolishes host cell invasion and survival in host macrophages.
Uncharacterized PPE family protein PPE62
Uncharacterized PPE family protein PPE36
HTH-type transcriptional regulatory protein RaaS
Reduces DNA binding affinity and ligand binding.
Decrease in heme binding. Strong decrease in heme binding; when associated with A-64.
Decrease in heme binding. Strong decrease in heme binding; when associated with A-90.
Potassium-transporting ATPase KdpC subunit
Probable glycine dehydrogenase (decarboxylating)
Lack of phosphoglycerate mutase activity.
ATP-dependent Clp protease ATP-binding subunit ClpC1
Probable nitrate/nitrite transporter NarK2
Medium/long-chain-fatty-acid--CoA ligase FadD17
Probable zinc-binding alcohol dehydrogenase Rv1895
Uncharacterized methyltransferase Rv0224c
HTH-type transcriptional repressor Rv1353c
Putative succinyl-diaminopimelate desuccinylase DapE
Phosphate transport system permease protein PstC 1
Probable long-chain-fatty-acid--AMP ligase FadD30
Phosphate transport system permease protein PstC 2
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Glutamate-1-semialdehyde 2,1-aminomutase
Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
Cytokinin riboside 5'-monophosphate phosphoribohydrolase
Succinate-semialdehyde dehydrogenase [NADP(+)] 1
Putative aminoglycoside phosphotransferase
Constitutive interaction with RsbW; leads to active SigF.
Alpha-ketoglutarate-dependent sulfate ester dioxygenase
No autophosphorylation, no phosphate transfer to DevR (DosR).
Oxygen sensor histidine kinase response regulator DosT
Decreases stability of the covalent enzyme-meropenem adduct.
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Coenzyme A biosynthesis bifunctional protein CoaBC
Uncharacterized PE-PGRS family protein PE_PGRS24
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0281
Putative acyl-[acyl-carrier-protein] desaturase DesA2
Does not affect phosphorylation. Lack of phosphorylation; when associated with A-171 and A-173.
Lack of phosphorylation; when associated with A-168 and A-171.
Lack of phosphorylation; when associated with A-168 and A-173.
Phosphate-specific transport system accessory protein PhoU homolog 1
Medium/long-chain-fatty-acid--CoA ligase FadD8
Acetolactate synthase large subunit IlvB1
Branched-chain-amino-acid aminotransferase
40% reduction in liposome disruption. 80% reduction; when associated with 2-T--G-10.
40% reduction in liposome disruption. 80% reduction; when associated with 86-T--A-95.
40% reduction in liposome disruption. 88% reduction in liposome disruption; when associated with E-17. Reduced liposome disruption; when associated with T-38.
88% reduction in liposome disruption; when associated with 25-T-A-26.
Bacteria no longer gain access to host cytoplasm via phagosomal rupture.
Does not restore virulence in an esxA deletion strain, decreased secretion of EsxA and EsxB.
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB (PubMed:16048998). Partially inhibits host TLR2-signaling (PubMed:17486091).
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB but no complex formed (PubMed:16048998). No effect on host TLR2-signaling.
Does not restore virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB.
NBD-linked protein does not fluoresce at acidic pH in presence of liposomes, this residue may face inwards in membranes away from lipids.
NBD-linked protein fluoresces at acidic pH in presence of liposomes, suggests this residue faces lipids.
NBD-linked protein moderately fluoresces at acidic pH in presence of liposomes, suggests this residue is partially within membrane.
No longer inhibits host TLR2-signaling (PubMed:17486091). Does not interact with host B2M, does not decrease B2M cell surface expression, no defects in antigen presentation (PubMed:25356553).
Partially restores virulence in an esxA deletion strain, wild-type secretion of EsxA and EsxB (PubMed:16048998). Partially inhibits host TLR2-signaling (PubMed:17486091).
Reduced liposome disruption; when associated with 25-T-A-26.
Wild-type virulence in an esxA deletion strain.
Wild-type virulence in an esxA deletion strain; protein should not be acetylated.
7-fold increase in holoenzyme adenylyl cyclase activity at pH 8.0.
Almost complete loss of adenylyl cyclase activity in the catalytic fragment.
Loss of adenylyl cyclase activity in the catalytic fragment.
Retains 10% adenylyl cyclase activity in the catalytic fragment.
HTH-type transcriptional regulator Rv1931c
Probable cystathionine beta-synthase Rv1077
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
GDP-mannose-dependent alpha-mannosyltransferase
Probable N-succinyldiaminopimelate aminotransferase DapC
Abolishes transferase activity. Decrease in TOH production. Almost loss of iso-TOH production.
Decrease in TOH production and almost loss of iso-TOH production. Almost loss of TOH and TOH production; when associated with F-51.
Does not affect TOH production. Strong decrease in iso-TOH production.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-81 or N-82.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-81 or N-85.
Significant decrease in phosphatase activity. Loss of phosphatase activity; when associated with N-82 or N-85.
Strong decrease in TOH and iso-TOH production. Almost loss of TOH and TOH production; when associated with F-90.
Leads to the appearance of shorter chain products, GPP and E,E-FPP, with only small amounts of GGPP produced.
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
Confers resistance to BTZ043 and PBTZ169. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, and only slightly affects catalytic efficiency. Is only partially inhibited by PBTZ169 at high concentrations, while totally inhibited by the non-covalent inhibitor Ty38c.
Confers resistance to BTZ043 and PBTZ169. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, but reduces catalytic efficiency by 4-fold. Is only partially inhibited by PBTZ169 at high concentrations, while totally inhibited by the non-covalent inhibitor Ty38c.
Confers resistance to BTZ043 and PBTZ169. Loss of covalent modification with BTZ043. Decreases M.tuberculosis cytotoxicity in macrophages. Does not affect binding affinity of the substrate to the enzyme, but reduces catalytic efficiency by 4-fold. Is only partially inhibited by PBTZ169 at high concentrations, while nearly totally inhibited by the non-covalent inhibitor Ty38c.
Decaprenylphosphoryl-beta-D-ribose oxidase
In a spontaneous TCA1-resistant mutant strain, but sensitive to BTZ.
In strain: TRC11; Ty38c-resistant mutant strain lacking Rv3406, but sensitive to moxifloxacin.
In strain: TRC12; Ty38c-resistant mutant strain lacking Rv3406, but sensitive to moxifloxacin.
Significantly less susceptible to Ty38c inhibition. 34-fold reduction in catalytic activity.
Significantly less susceptible to Ty38c inhibition. 7-fold reduction in catalytic activity.
Uncharacterized SURF1-like protein Rv2235
Uncharacterized PPE family protein PPE44
2.5-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
2.7-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
3-fold decrease in binding of POA, decreased binding of tmRNA (expressed as residues 285-481).
Complete loss of POA binding, significantly decreased binding of tmRNA (expressed as residues 285-481).
Does not bind POA. Binds tmRNA less strongly than wild-type, binding is not disrupted by POA.
Trehalose transport system permease protein SugA
Putative diacyglycerol O-acyltransferase Rv0221
Seven-bladed beta-propeller protein Rv1057
Salicyl-AMP ligase / salicyl-S-ArCP synthetase
Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
Loss of more than 70% enzymatic activity.
Glutamyl-tRNA(Gln) amidotransferase subunit C
Cannot be phosphorylated. No change in activity.
Catalyzes the formation of acyl-CoA; when associated with A-383.
Catalyzes the formation of acyl-CoA; when associated with A-481.
Confers resistance to the coumarin inhibitors.
Long-chain-fatty-acid--AMP ligase FadD32
Phosphomimetic mutant. 55% decrease in activity.
Blue shift in fluorescence emission, possible decrease in -10 promoter recognition.
3-oxoacyl-[acyl-carrier-protein] synthase 1
Mimics structural changes caused by acyl-enzyme formation.
Uncharacterized PPE family protein PPE45
Putative fluoride ion transporter CrcB 2
Ribosomal RNA small subunit methyltransferase G
No effect on PbpB (PBP3, FtsI) processing.
Heat-inducible transcription repressor HrcA
Uncharacterized GMC-type oxidoreductase Rv1279
Reduces lipoamide dehydrogenase activity by 82%.
Reduces lipoamide dehydrogenase activity by 89%.
Reduces lipoamide dehydrogenase activity by 91%.
Reduces lipoamide dehydrogenase activity by 94%.
Reduces lipoamide dehydrogenase activity by 95%.
Reduces lipoamide dehydrogenase activity by 96%.
F420H(2)-dependent quinone reductase Rv1261c
Uncharacterized HTH-type transcriptional regulator Rv2912c
Succinate--CoA ligase [ADP-forming] subunit beta
Unable to dehydrogenate pregnene-carboxyl-CoA ester.
12-fold decrease in catalytic efficiency with NADH as substrate. 26-fold decrease in catalytic efficiency; when associated with A-146.
2.2-fold decrease in catalytic efficiency with NADH as substrate. 26-fold decrease in catalytic efficiency; when associated with A-445.
3-oxoacyl-[acyl-carrier-protein] reductase [NADH]
Drastic reduction of ketoreductase activity.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0731c
17.8-fold decrease in catalytic efficiency.
2.6-fold decrease in catalytic efficiency.
2.7-fold decrease in catalytic efficiency.
3.3-fold decrease in catalytic efficiency.
4.7-fold decrease in catalytic efficiency.
44.5-fold decrease in catalytic efficiency.
8.9-fold decrease in catalytic efficiency.
Uncharacterized HTH-type transcriptional regulator Rv0767c
Does not inhibit growth, forms complex with VapB4.
Probable protein-export membrane protein SecG
DNA-directed RNA polymerase subunit alpha
F420H(2)-dependent quinone reductase Rv1558
Loss of fatty acyl dehydrogenase activity.
14.5-fold decrease in catalytic efficiency.
24-fold decrease in catalytic efficiency.
3.1-fold decrease in catalytic efficiency.
3.5-fold decrease in catalytic efficiency.
4.4-fold decrease in catalytic efficiency.
6.6-fold decrease in catalytic efficiency.
(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase
Putative trans-acting enoyl reductase Rv2449c
Decreased TLR2 agonist activity, cannot acquire lipid from mycobacterial extracts, cavity entrance and size decrease. Binds PIM6, LM and ManLAM 10-100-fold less well than wild-type, binds de-acylated LM and ManLAM as well as wild-type. Decreased ability to transfer triacylglyceride between lipid bilayers.
Sec translocon accessory complex subunit YajC
Probable biotin-dependent acyl-coenzyme A carboxylase beta3 subunit
Doxorubicin resistance ATP-binding protein DrrA
About 5% of wild-type phosphatase activity.
Almost completely abolished phosphatase activity.
Disrupts dimerization of the enzyme, which exists as a monomer and has lost its ability to perform dephosphorylation.
Loss of GTP-synthesizing activity and lack of autophosphorylation. Does not interact with Ndk.
Partial loss of GTP-synthesizing activity and decrease in autophosphorylation. Loss of GTP-synthesizing activity and lack of autophosphorylation; when associated with A-115.
Partial loss of GTP-synthesizing activity and decrease in autophosphorylation. Loss of GTP-synthesizing activity and lack of autophosphorylation; when associated with A-247.
Partial loss of GTP-synthesizing activity.
Attenuates the ability to bind salicylate.
HTH-type transcriptional repressor Rv2887
Ribonucleoside-diphosphate reductase subunit alpha
Uncharacterized ABC transporter ATP-binding protein Rv0073
Uncharacterized ABC transporter ATP-binding protein Rv1281c
Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-? in acceptor proteins); alternate
Uncharacterized PPE family protein PPE49
Putative inactive phenolphthiocerol synthesis polyketide synthase type I Pks1
Transposase for insertion sequence element IS1557
Involved in ionization of N3 of dUMP, leading to its activation
Loss of catalytic activity since it is not able to complement an E.coli thyA deletion mutant.
Still able to complement an E.coli thyA deletion mutant.
Loss of autophosphorylation. Partially complements an M.smegmatis mtrB disruption, cells are still filamentous and bulged but the protein targets to septa.
3-fold decrease in catalytic activity, and 2-fold decrease in substrate affinity.
4-fold decrease in catalytic activity with Mg(2+) as cofactor. 3-fold increase in catalytic activity with Zn(2+) as cofactor. Shifts the pH for optimal activity to 8.5.
Nearly no effect on catalytic activity with Mg(2+) as cofactor. 10-fold increase in catalytic activity with Zn(2+) as cofactor.
Probable peptidyl-prolyl cis-trans isomerase B
Probable 3-hydroxyisobutyrate dehydrogenase
Probable transport accessory protein MmpS1
Probable transcriptional regulator WhiB7
Holliday junction ATP-dependent DNA helicase RuvA
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3787c
Exopolysaccharide phosphotransferase CpsY
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Tuberculostearic acid methyltransferase UfaA1
Uncharacterized PPE family protein PPE37
Shows higher efflux activity. Causes clinically relevant high-level drug resistance to pyrazinamide (PZA), isoniazid (INH) and streptomycin (SM).
Shows higher efflux activity. Causes clinically relevant low-level drug resistance to pyrazinamide (PZA), isoniazid (INH) and streptomycin (SM).
Uncharacterized PPE family protein PPE29
Pyridoxal 5'-phosphate synthase subunit PdxT
Probable phosphatidylglycerophosphate synthase
No in vitro phosphorylation by PknB; when associated with A-106.
No in vitro phosphorylation by PknB; when associated with A-26.
Uncharacterized HTH-type transcriptional regulator Rv0472c
Uncharacterized PE-PGRS family protein PE_PGRS3
Putative FAD-containing monooxygenase MymA
Probable diacyglycerol O-acyltransferase Tgs4
Uncharacterized ABC transporter ATP-binding protein Rv2564
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3767c
TVP38/TMEM64 family membrane protein Rv1491c
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
UDP-N-acetylenolpyruvoylglucosamine reductase
Probable lipoprotein aminopeptidase LpqL
(7aS)-7a-methyl-1,5-dioxo-2,3,5,6,7,7a-hexahydro-1H-indene-carboxyl-CoA hydrolase
Uncharacterized MFS-type transporter Rv2456c
Small but detectable amount of cYY-forming activity.
Small but detectable amount of catalytic activity (200-fold decrease).
Phenolphthiocerol/phthiocerol polyketide synthase subunit C
Diacylglycerol acyltransferase/mycolyltransferase Ag85C
Do not form alpha,alpha-trehalose dimycolate (TDM, cord factor) and alpha,alpha-trehalose monomycolate (TMM).
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase epsilon subunit
Arabinogalactan biosynthesis recruiting protein Rv3789
Probable histone acetyltransferase Rv0428c
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl:CoA ligase
Lack of autophosphorylation. Decreases affinity for FhaB.
Reduces activity and autophosphorylation. Decreases interaction with GarA.
Abolishes catalytic activity on AG substrates.
Decreases catalytic activity on AG substrates, leads to a change in the number of acetylated sites.
Nearly abolishes catalytic activity on AG substrates.
No effect on catalytic activity on AG substrates. Prevents artifactual CoA adduct formation during crystallization.
F420-dependent hydroxymycolic acid dehydrogenase
Methylated-DNA--protein-cysteine methyltransferase
Alpha-(1->6)-mannopyranosyltransferase A
Phosphoribosylformylglycinamidine synthase subunit PurL
Putative diacyglycerol O-acyltransferase Rv3480c
Ribonucleoside-diphosphate reductase subunit beta nrdF1
Causes a decrease in ammonia channel efficiency to 70% compared with wild-type enzyme.
Lack of glutamine-dependent activity. Retains 30% of ammonia-dependent activity.
Lack of glutamine-dependent activity. Retains 90% of ammonia-dependent activity.
Lack of glutamine-dependent and ammonia-dependent activities.
Proton acceptor; for glutaminase activity
Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase
Abolishes phosphorylation. No loss of DNA-binding activity in vitro.
Uncharacterized PPE family protein PPE61
Putative fluoride ion transporter CrcB 1
ESX-3 secretion-associated protein EspG3
20% ligase activity for singly nicked dsDNA, normal N6-AMP-lysine intermediate formed, no effect on phosphodiester bond formation, no effect on DNA polymerase.
Better than wild-type DNA-binding and filling on single nt gaps, impaired gap filling on more complicated templates (in Pol domain).
Binds DNA, no formation of DNA end-bridging complex, no polymerase activity. Significantly decreased ability to fill in 2 nt gaps (in Pol domain).
Binds DNA, no gap-filling (in Pol domain).
Loss of DNA-binding, no polymerase activity, no effect of gap-filling (in Pol domain).
Loss of adenyltransferase activity, no N6-AMP-lysine formation and loss of ligase activity. No effect on phosphodiester bond formation on pre-adenylated nicked DNA, or on DNA polymerase.
Loss of polymerase activities, no DNA repair (in Pol domain).
Multifunctional non-homologous end joining DNA repair protein LigD
N6-AMP-lysine intermediate; for ligase activity
No ligase of singly nicked dsDNA activity, 25% N6-AMP-lysine intermediate formed, decreased phosphodiester bond formation, no effect on DNA polymerase.
No ligase of singly nicked dsDNA activity, no N6-AMP-lysine intermediate formed, decreased phosphodiester bond formation on pre-adenylated nicked DNA, no effect on DNA polymerase.
No ligase of singly nicked dsDNA activity, no N6-AMP-lysine intermediate formed, no phosphodiester bond formation on pre-adenylated nicked DNA, no effect on DNA polymerase.
On substrate with 5'-phosphate, 1 base pair (bp) complementarity and 1 nucleotide (nt) gap, greatly reduced DNA-binding and gap-filling. Barely detectable activity on substrate with high complementarity and 3'-overhang (in Pol domain).
On substrate with 5'-phosphate, 1 bp complementarity and 1 nt gap, greatly reduced DNA-binding and gap-filling. Barely detectable activity on substrate with high complementarity and 3'-overhang (in Pol domain).
On substrate with 5'-phosphate, 1 bp complementarity and 1 nt gap, greatly reduced DNA-binding and gap-filling. No activity on substrate with high complementarity and 3'-overhang (in Pol domain).
Wild-type filling on single nt gaps, impaired gap filling on more complicated templates (in Pol domain).
tRNA (adenine(58)-N(1))-methyltransferase TrmI
Enhances catalytic activity and stability.
NADPH-dependent stearoyl-CoA 9-desaturase
Uncharacterized MFS-type transporter Rv0849
Loss of activity; when associated with A-179.
Loss of activity; when associated with A-181.
Putative envelope-preserving system protein Rv2742c
Probable multidrug-efflux transporter Rv1634
Uncharacterized PE-PGRS family protein PE_PGRS34
Decaprenyl-phosphate phosphoribosyltransferase
No DPPR synthase activity and the protein is not expressed in the membrane.
Only small effects on the DPPR synthase activity.
Substantially reduced the DPPR synthase activity.
The DPPR synthase activity is strongly diminished.
Bifunctional F420 biosynthesis protein FbiB
Ribosomal RNA small subunit methyltransferase I
4-hydroxy-tetrahydrodipicolinate synthase
Exists as dimer in solution. Has similar activity to the wild-type enzyme. Slight decrease in affinity for substrates.
Loss of DNA-binding, in fragment 66-112.
Loss of activity. Can form dimers but does not bind DNA.
No change in activity. Can still bind DNA and form dimers.
Reduced DNA-binding, in fragment 66-112.
3-methyl-2-oxobutanoate dehydrogenase subunit alpha
Imidazole glycerol phosphate synthase subunit HisF
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Probable hercynylcysteine sulfoxide lyase
Probable molybdenum cofactor guanylyltransferase
Sec-independent protein translocase protein TatA
Putative Rieske 2Fe-2S iron-sulfur protein Rv3818
Appears to be functional by complementation study.
Appears to be non-functional by complementation study.
Proton acceptor; for dehydratase activity
Uncharacterised methyltransferase Rv1498c
Uncharacterized SufE-like protein Rv3284
Abolishes complementation of the Rv3671c mutant. It does not exhibit autocleavage activity.
Significant decrease of protease activity.
Putative diacyglycerol O-acyltransferase Rv2285
Probable cytochrome c oxidase polypeptide 4
Loss of proteasome proteolytic activity. This mutant enables optimal growth in vitro and in vivo, confers RNI resistance but is not sufficient for persistence in mice.
Removed in mature form; by autocatalysis
Molybdopterin synthase catalytic subunit 1
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Probable transcriptional regulatory protein PdtaR
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-59 and S-62.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-59 and S-68.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-37; S-62 and S-68.
Partial loss of the Fe-S center. Complete loss of center and of disulfide reductase; when associated with S-59; S-62 and S-68.
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
4-hydroxyphenylalkanoate adenylyltransferase
Does not affect secretion, but inhibits disulfide bond formation. Alters mycobacterial cell wall integrity and significantly attenuates virulence.
Impairs EsxA and EsxB secretion in vitro. Modest impact on EspD stability and secretion. Attenuated in cellular models of tuberculosis infection.
Severely destabilizes EspA and blocks ESX-1 secretion in vitro, but does not impact the cytotoxicity in cellular models of infection. Can induce pro-inflammatory responses in THP-1 cells and is as virulent as wild-type during the acute phase of infection in mice.
Severely destabilizes EspA and blocks ESX-1 secretion in vitro. Attenuated in cellular models of tuberculosis infection.
Probable acetyl-CoA acetyltransferase, propeptide removed
Putative F420H(2)-dependent quinone reductase Rv3178
Uncharacterized HTH-type transcriptional regulator Rv1828
3-isopropylmalate dehydratase large subunit
Incorrect processing of pre-crRNA, binds pre-crRNA.
Incorrect processing of pre-crRNA, no longer binds pre-crRNA.
Uncharacterized PPE family protein PPE58
Molybdenum transport system permease protein ModB
Remains cell wall associated, no difference in gel migration, upon overexpression in M.smegmatis.
1200-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
1400-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
1700-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
Uncharacterized PPE family protein PPE32
HTH-type transcriptional regulator MmpR5
In CFZ-R1; leads to increased expression of mmpL5 and partial resistance to both clofazimine and bedaquiline.
Cyclopropane mycolic acid synthase MmaA2
Mycobactin import ATP-binding/permease protein IrtB
Phosphate-specific transport system accessory protein PhoU homolog 2
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Polyprenol-phosphate-mannose-dependent alpha-(1-2)-phosphatidylinositol mannoside mannosyltransferase
Uncharacterized HTH-type transcriptional regulator Rv0043c
Medium/long-chain-fatty-acid--[acyl-carrier-protein] ligase MbtM
Putative diacyglycerol O-acyltransferase Rv3087
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit A
Cyclic pyranopterin monophosphate synthase 3
Putative 8-amino-7-oxononanoate synthase 2
Ribosomal RNA small subunit methyltransferase A
Putative bifunctional exonuclease/endonuclease protein Rv2191
Uncharacterized PE-PGRS family protein PE_PGRS20
Glutamyl-tRNA(Gln) amidotransferase subunit A
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Bifunctional purine biosynthesis protein PurH
Long-chain-fatty-acid--AMP ligase FadD23
ATP-dependent 6-phosphofructokinase isozyme 2
Fluoroquinolones export permease protein Rv2687c
Copper-sensing transcriptional repressor CsoR
Decrease in ability to dissociate from DNA in the presence of copper.
Loss of ability to bind copper; loss of ability to dissociate from DNA in the presence of copper.
Loss of ability to bind to DNA; when associated with A-15 and A-36.
Loss of ability to bind to DNA; when associated with A-15 and A-52.
Loss of ability to bind to DNA; when associated with A-36 and A-52.
Reduces FtsZ polymerization and GTP hydrolysis. Does not affect the structure or the stability of the unpolymerized FtsZ.
Putative acyl-[acyl-carrier-protein] desaturase DesA1
N-acetylmuramoyl-L-alanine amidase Rv3717
Putative flavin-containing monoamine oxidase AofH
3-oxo-4,17-pregnadiene-20-carboxyl-CoA hydratase alpha subunit
Potassium-transporting ATPase ATP-binding subunit
Carbamoyl-phosphate synthase large chain
Terminal beta-(1->2)-arabinofuranosyltransferase
D-inositol 3-phosphate glycosyltransferase
ATP-dependent Clp protease adapter protein ClpS
Putative peptidoglycan hydrolase Rv2525c
Sulfate/thiosulfate import ATP-binding protein CysA
10-fold decrease in enzymatic activity with NAM as substrate, and 21-fold decrease in enzymatic activity with PZA as substrate. The mutant has a low metal ion content.
100-fold decrease in enzymatic activity with NAM or PZA as substrate. The mutant has a low metal ion content.
112-fold decrease in enzymatic activity with NAM as substrate, and 164-fold decrease in enzymatic activity with PZA as substrate. The mutant has no detectable iron and manganese.
2.4-fold decrease in enzymatic activity with NAM or PZA as substrate. No effect on metal ion content.
3000-fold decrease in enzymatic activity with NAM as substrate, and 410-fold decrease in enzymatic activity with PZA as substrate. The mutant has no detectable iron and manganese.
5-fold decrease in enzymatic activity with NAM as substrate, and 3-fold decrease in enzymatic activity with PZA as substrate. No effect on metal ion content.
Loss of enzymatic activity. No effect on metal ion content.
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Cholesterol ring-cleaving hydrolase IpdB subunit
Decrease in activity; when associated with A-106; A-109 and A-128.
Decrease in activity; when associated with A-106; A-109 and A-131.
Decrease in activity; when associated with A-106; A-128 and A-131.
Decrease in activity; when associated with A-109; A-128 and A-131.
Decreases inhibition by AX20017; when associated with S-87.
Decreases inhibition by AX20017; when associated with S-92.
Lack of activity; when associated with S-106; S-109 and S-128.
Lack of activity; when associated with S-106; S-109 and S-131.
Lack of activity; when associated with S-106; S-128 and S-131.
Lack of activity; when associated with S-109; S-128 and S-131.
Lack of phosphorylation. Does not affect kinase activity.
It promotes stronger allosteric interactions.
Lower catalytic efficiency. A perturbation of the allosteric interactions is observed when NAD is used as substrate.
Possesses 30% of the activity compared to the wild-type enzyme. While mutant affects the catalytic efficiency, it does not alter the binding affinity for ATP and poly(P). It causes a decrease in the affinity for NAD and alters the allosteric interactions mediated by the dinucleotide, both in the presence of poly(P) and ATP.
Mycocerosic acid synthase-like polyketide synthase
2-fold decrease in glycohydrolase activity. Intermediate cytotoxicity.
Decreases glycohydrolase activity and cytotoxicity in macrophages.
Lack of glycohydrolase activity and of cytotoxicity; when associated with K-822.
Lack of glycohydrolase activity and of cytotoxicity; when associated with N-792.
Unfolded. Lack of glycohydrolase activity. Abolishes toxicity.
ESX-1 secretion-associated protein EspG1
Uncharacterized ABC transporter permease Rv0072
Probable glycerol-3-phosphate dehydrogenase 2 [NAD(P)+]
Type II NADH:quinone oxidoreductase NdhA
Almost completely abolishes phosphorylation by PknH.
Uncharacterized HTH-type transcriptional regulator Rv0681
Decreases affinity for Mn3 by 4-fold, no effect on hydrolysis of p-nitrophenyl phosphate.
No change in affinity for Mn3, no effect on hydrolysis of p-nitrophenyl phosphate.
Serine/threonine protein phosphatase PstP
Cyclic pyranopterin monophosphate synthase 2
Bifunctional oligoribonuclease and PAP phosphatase NrnA
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG
Transcription termination/antitermination protein NusA
Considerably reduced interaction with SigE. Complete loss of interaction; when associated with A-70.
Considerably reduced interaction with SigE. Complete loss of interaction; when associated with A-73.
Loss of phosphorylation, no protein degradation.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0830
Uncharacterized NTE family protein Rv1063c
GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase
Uncharacterized PPE family protein PPE65
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase beta6 subunit
Diacylglycerol acyltransferase/mycolyltransferase Ag85B
Probable transcriptional regulatory protein NarL
14-fold reduction in catalytic activity. 10-fold decrease in affinity for ATP but no change in affinity for UTP. Causes resistance to the anti-tubercular compounds 7947882 and 7904688. Catalytic activity cannot be inhibited by the active metabolite of 7947882.
Probable transport accessory protein MmpS3
Can catalyze the hydrolysis of palmitoyl-CoA, but cannot produce cyclic polyketide products.
Methyl-branched alkylpyrone synthesis polyketide synthase-like Pks11
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2
Uncharacterized PPE family protein PPE13
3-isopropylmalate dehydratase small subunit
Cells unable to export EsxB, missing transmembrane domain.
1,4-dihydroxy-2-naphthoate octaprenyltransferase
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Uncharacterized PPE family protein PPE66
30% decrease in activity with pNP-octanoate and vinyl-caprylate as substrates.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. 17-18% increase in activity with VC6 or VC8 as substrates. Aquires phospholipase A activity and shows cytotoxic effects on macrophages.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. 31% and 25% decrease in activity with 6 carbon atoms vinyl esters (VC6) or 8 carbon atoms vinyl esters (VC8) as substrates. Enhances lipase activity on triglycerides. Aquires phospholipase A activity and shows cytotoxic effects on macrophages.
Shows a strong preference for substrates with a medium chain length ranging between 6 and 8 carbon atoms. Loses 99% and 80% of activity with VC6 or VC8 as substrates. Loss of lipase activity.
F420-dependent glucose-6-phosphate dehydrogenase
Probable ferredoxin/ferredoxin--NADP reductase
Multidrug efflux ATP-binding/permease protein Rv0194
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Uncharacterized RNA pseudouridine synthase Rv1711
Uncharacterized ABC transporter permease Rv2563
Adenosylcobinamide-GDP ribazoletransferase
Nucleophile; cysteine thiosulfonate intermediate
Lack of protease activity. Has hyperactivated ESX-1 secretion during macrophage infection, resulting in increased innate immune signaling. Secretes full-length EspB.
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
20-fold decrease in the rate of INH-NAD adduct formation. Exhibits significantly reduced affinity for INH (KM is increased by 43-fold).
Exhibits 1.7-fold decreased catalytic efficiency for the activation of INH.
Exhibits 2-fold increased affinity for INH.
Exhibits 8-fold increased catalytic efficiency for the activation of INH (INH-NAD formation). Possesses an enlarged substrate access channel.
Nearly no effect on the kinetic parameters for the activation of INH.
Nearly no effect on the kinetic parameters for the catalase and peroxidase activity. Activates INH and mediates InhA inactivation as efficiently as wild-type.
Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255); alternate
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with Trp-107)
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107); alternate
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Transcription antitermination protein NusB
The measured mass is that of a dimer, plus 2 cadmium ions.
Molybdopterin synthase catalytic subunit 2
Decreases ATPase activity. Abolishes autophosphorylation. Can still be phosphorylated by PknD.
Does not affect ATPase activity. Abolishes autophosphorylation.
Does not affect autophosphorylation. 10-fold decrease in phosphatase activity.
Leads to the accumulation of the autophosphorylated protein. 10-fold decrease in phosphatase activity.
Strong decrease in ATPase activity. Abolishes autophosphorylation.
Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
Completely abolishes enzymatic activity.
Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.
Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
3-phosphoshikimate 1-carboxyvinyltransferase
Uncharacterized glycosyltransferase Rv1514c
2-oxoglutarate oxidoreductase subunit KorA
Putative diacyglycerol O-acyltransferase Rv1425
Sec-independent protein translocase protein TatB
Dormancy associated translation inhibitor
Glyceraldehyde-3-phosphate dehydrogenase
Same dehydrogenase activity compared to the wild-type.
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase
60% decrease in phosphorylation by PknH.
D-alanyl-D-alanine carboxypeptidase DacB1
11-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Does not affect nitrosylation. 80% decrease in phosphatase activity.
Lack of nitrosylation. Does not affect phosphatase activity.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. 36-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. Reduces its effect on phagolysosome fusion in infected macrophages. Does not affect nitrosylation.
Loss of phosphatase activity. Inhibits its dephosphorylation activity on human VPS33B. Reduces its effect on phagolysosome fusion in infected macrophages. Still binds V-ATPase subunit H. 380-fold decrease in catalytic efficiency toward pNPP at pH 7.0.
Loss of phosphatase activity; when associated with E-128.
Loss of phosphatase activity; when associated with E-129.
Low molecular weight protein-tyrosine phosphatase A
Mutant can be phosphorylated. Lack of phosphorylation and loss of phosphatase activity; when associated with A-128.
Mutant can be phosphorylated. Lack of phosphorylation and loss of phosphatase activity; when associated with A-129.
No longer binds to ubiquitin. Abolishes the Jnk- and p38-dependent production of TNF and IL-1-beta in macrophages. Also abolishes the PtpA-mediated inhibition of phagosome acidification.
Retains phosphatase activity but does not bind V-ATPase subunit H. Fails to inhibit V-ATPase trafficking to phagosomes and shows impaired intracellular survival.
Slight decrease in phosphatase activity.
Strong decrease in phosphatase activity.
Uncharacterized glycosyltransferase Rv1524
3-ketosteroid-9-alpha-monooxygenase, ferredoxin reductase component
Putative phosphoribosyl transferase Rv0571c
Lack of phosphorylation by PknD. Reduced oprA induction in response to osmotic stress.
Completely abolishes the diaminopimelate epimerase activity.
Strongly reduces the diaminopimelate epimerase activity.
Uncharacterized PPE family protein PPE16
Uncharacterized methyltransferase Rv0089
Uncharacterized methyltransferase Rv3342
Meromycolate extension acyl carrier protein
Uncharacterized MFS-type transporter Rv0037c
Precorrin-6Y C(5,15)-methyltransferase [decarboxylating]
RNA polymerase-binding transcription factor CarD
Weakens interaction with RpoB, decreases growth rate and virulence, and increases rifampicin sensitivity. Abolishes interaction with RpoB; when associated with E-25.
Weakens interaction with RpoB. Abolishes interaction with RpoB; when associated with E-47.
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Cytochrome bc1 complex Rieske iron-sulfur subunit
Electron transfer flavoprotein subunit beta
Does not block ESX-1-mediated secretion during ATP depletion. Lack of ATP-binding.
Encapsulin nanocompartment protein Rv1762c
Uncharacterized HTH-type transcriptional regulator Rv1556
Molybdopterin synthase sulfur carrier subunit
Uncharacterized NTE family protein Rv2565
Decreased ConA binding; altered protein processing yields 21 and 16 kDa forms in M.smegmatis.
Decreased ConA binding; altered protein processing yields 21 and 17 kDa forms in M.smegmatis.
No ConA binding; altered protein processing yields 16 kDa soluble form starting on residue 40 in M.smegmatis. Protein poorly released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Protein not exported from cytoplasm, not released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Protein not released into host cytoplasm (uses recombinant M.vaccae to infect mouse macrophages).
Alpha-(1->6)-mannopyranosyltransferase Rv1459c
Fluoroquinolones export permease protein Rv2686c
ABC transporter ATP-binding/permease protein Rv1747
Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
Strong decrease in phosphorylation. Decreases interaction with PknF.
Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0893c
Does not affect interaction with NCF2 and ROS production.
Does not interact with NCF2 and cannot inhibit ROS production.
Putative membrane protein insertion efficiency factor
It is catalytically catastrophic, but strongly activated by AroG.
The catalytic efficiency and the affinity are 5 and 3-fold lower than the wild-type. The activation by AroG is 10-fold lower than the wild-type.
The catalytic efficiency and the affinity are higher than the wild-type. The activation by AroG is 20-fold lower than the wild-type.
The catalytic efficiency and the affinity are identical to the wild-type. The activation by AroG is 10-fold lower than the wild type.
The catalytic efficiency and the affinity are slightly modified. The activation by AroG is 2-fold lower than the wild-type.
Uncharacterized MFS-type transporter Rv1250
Probable inositol 1-monophosphatase ImpA
Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
Does not show activity with small, medium or long acyl chains.
Long-chain-fatty-acid--CoA ligase FadD13
No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
Reduction of binding affinity for fatty acids.
Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.
Does not bind 4Fe-4S cluster, binds DNA.
For fully alkylated recombinant protein tagged at both termini.
Loss of 4Fe-4S cluster, loss of DNA-binding.
Loss of DNA-binding, still binds 4Fe-4S cluster.
Decaprenyl-phosphate N-acetylglucosaminephosphotransferase
When expressed from this site no longer acts as a TLR2 agonist, suggests active protein is acylated. No glycosylated form detected.
3'-phosphoadenosine 5'-phosphate phosphatase
Less than 2% of wild-type IMPase and FBPase activities.
Less than 3% of wild-type IMPase and FBPase activities.
No effect on IMPase and FBPase activities. Much less sensitive to inhibition by Li(+).
Uncharacterized HIT-like protein Rv0759c
[Acyl-carrier-protein] phosphodiesterase PptH
Omega-hydroxy-beta-dihydromenaquinone-9 sulfotransferase Stf3
HTH-type transcriptional regulator Mce2R
Loss of phosphorylation by PknG/PknK/PknJ.
Sec-independent protein translocase protein TatC
Ribonucleoside-diphosphate reductase subunit beta nrdF2
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
Putative RNA-guided DNA endonuclease Rv2885c
ESX-5 secretion-associated protein EspG5
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv1729c
3-methyl-2-oxobutanoate hydroxymethyltransferase
40% of the wild-type enzyme activity at pH 7.5 and 27% of the wild-type enzyme activity at pH 4.
Less than 1% of the wild-type enzyme activity.
Uncharacterized HTH-type transcriptional regulator Rv1395
Significantly decreased beta-lactamase and RNase activity.
Gly-cisPro motif, important for rejection of L-amino acids
50-fold reduction in activity and slight reduction in the affinity for beta-alanine. 30- and 40-fold decrease in adenylate formation and pantothenate formation, respectively.
More than 1000-fold reduction in activity and 120-fold decrease in adenylate formation. The enzymatic activity and the affinity for beta-alanine can be increased 10- and 3-fold, respectively, by alkylation of cysteine of mutant C-160.
More than 1000-fold reduction in activity and 45-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 50-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 50-fold decrease in the affinity for ATP.
More than 1000-fold reduction in activity and 52-fold decrease in adenylate formation.
More than 1000-fold reduction in activity and 60-fold decrease in adenylate formation. 10-fold decrease in the affinity for ATP.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-23; A-56 and A-62.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-33; A-53 and A-56.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-33; A-53 and A-62.
No 4Fe-4S cluster assembly, does not complement growth defects; when associated with A-53; A-56 and A-62.
Redox- and pH-responsive transcriptional regulator WhiB3
Does not affect activity and regulation by metal ions.
Uncharacterized HTH-type transcriptional regulator Rv0081
Abolishes homodimerization and strongly decreases enzyme activitywhen associated with E-296 and R-363.
Almost complete loss of enzyme activity.
Promotes formation of a domain-swapped dimer. Abolishes homodimerization and strongly decreases enzyme activity; when associated with E-296 and C-365.
Strongly decreased enzyme activity. Abolishes homodimerization and strongly decreases enzyme activity; when associated with R-363 and C-365.
Loss of phosphorylation; when associated with A-168.
Loss of phosphorylation; when associated with A-183.
Holliday junction ATP-dependent DNA helicase RuvB
Probable soluble pyridine nucleotide transhydrogenase
Phosphoribosylaminoimidazole-succinocarboxamide synthase
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1
Phenolphthiocerol/phthiocerol polyketide synthase subunit E
Uncharacterized tRNA/rRNA methyltransferase Rv3579c
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Decreases activity and alters substrate specificity.
Probable transcriptional regulatory protein Rv2603c
Putative short-chain type dehydrogenase/reductase Rv0148
Decreased apparent weight in M.smegmatis, a portion of the protein is protected from extracellular trypsin.
Putative phthiocerol dimycocerosate transporter LppX
Uncharacterized PPE family protein PPE28
Probable glycerophosphodiester phosphodiesterase 2
Uncharacterized PPE family protein PPE19
Probable biotin-dependent acyl-coenzyme A carboxylase beta2 subunit
Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX
Uncharacterized formamidopyrimidine-DNA glycosylase-like protein
Uncharacterized PPE family protein PPE24
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3399
Phosphoribosylglycinamide formyltransferase
Cysteine persulfide intermediate; for sulfurtransferase activity
Glycyl thioester intermediate; for adenylyltransferase activity
Probable adenylyltransferase/sulfurtransferase MoeZ
Altered association with RAC components.
Bifunctional (p)ppGpp synthase/hydrolase RelA
Uncharacterized PPE family protein PPE25
Uncharacterized ABC transporter permease protein Rv0987
N-acetyl-gamma-glutamyl-phosphate reductase
Partial loss of phosphorylation. Decrease in activity.
Causes a probable loss of enzymatic activity.
Biotin-dependent acetyl-/propionyl-coenzyme A carboxylase beta5 subunit
Phthioceranic/hydroxyphthioceranic acid synthase
Probable diacyglycerol O-acyltransferase tgs2
CDP-diacylglycerol--serine O-phosphatidyltransferase
Uncharacterized PPE family protein PPE38
Probable peptidoglycan biosynthesis protein MviN
2-oxoglutarate oxidoreductase subunit KorB
Probable L-lysine-epsilon aminotransferase
tRNA N6-adenosine threonylcarbamoyltransferase
Cyclic pyranopterin monophosphate synthase 1
Completely abolishes the interaction with the aliphatic tail of the substrate lysine.
Induces autoacetylation of the new lysine in the absence of cAMP.
Substantially decreases the interaction with the aliphatic tail of the substrate lysine.
Substantially decreases the interaction with the aliphatic tail of the substrate lysine. Markedly reduced activity, with an altered pH-rate profile and abolishes direct interactions with R-184.
Uncharacterized Na(+)/H(+) exchanger Rv2287
Adopts a conformation almost identical to the wild-type.
Decreases affinity for ATP and autophosphorylation efficiency.
Decreases affinity for ATP and autophosphorylation efficiency. Decreases interaction with PtpA.
Lack of autophosphorylation. Decreases interaction with PtpA. Cannot phosphorylate and activate PtpA.
Significant decrease in ATP binding affinity and autophosphorylation efficiency. Decreases interaction with PtpA.
Loss of interaction with the proteasome subunit PrcA.
Acetolactate synthase large subunit IlvG
Completely disrupts protein function, as shown by the dramatic decrease of RNA levels for the moa1 locus.
Has no deleterious effect on the transcription of the moa1 locus.
Shows reduced transcription of the moa1 locus.
Shows significantly reduced transcription of the moa1 locus.
Transcriptional regulatory protein MoaR1
Abolishes interaction with Rv1747. Impairs autokinase activity.
Decreases interaction with Rv1747. Decreases autokinase activity.
Loss of kinase activity. Abolishes interaction with Rv1747.
Phenolphthiocerol/phthiocerol polyketide synthase subunit B
Putative quercetin 2,3-dioxygenase Rv0181c
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Abolishes diacylglycerol acyltransferase activity.
Diacylglycerol acyltransferase/mycolyltransferase Ag85A
Interchain (with C-61 in AhpC); in linked form
Loss of activity. Loss of interchain disulfide bond with AhpC.
Reduces protein stability. No effect on catalytic activity.
Hypoxanthine-guanine phosphoribosyltransferase
Probable monoacyl phosphatidylinositol tetramannoside-binding protein LpqW
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Leads to decreased interaction with PpsE, but does not decrease MmpL7 activity in vivo.
Phthiocerol dimycocerosate exporter MmpL7
Malonyl CoA-acyl carrier protein transacylase
Nearly abolishes pupylation and dramatically stabilizes this proteasome substrate in wild-type mycobacteria.
Putative maltooligosyl trehalose synthase
Galactan 5-O-arabinofuranosyltransferase
Uncharacterized HTH-type transcriptional regulator Rv1830
Medium/long-chain-fatty-acid--[acyl-carrier-protein] ligase FadD10
Putative HTH-type transcriptional regulator Rv1287
Phosphatidylinositol mannoside acyltransferase
Considerable loss of chaperone activity.
Retains chaperone activity; when associated with A-27, A-50 and A-53.
Retains chaperone activity; when associated with A-27, A-50 and A-59.
Retains chaperone activity; when associated with A-27, A-53 and A-59.
Retains chaperone activity; when associated with A-50, A-53 and A-59.
Putative succinyl-CoA transferase Rv0802c
Medium/long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligase
Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase
Putative thiosulfate sulfurtransferase SseB
Can form a disulfide bond; when associated with C-440. Decreased autophosphorylation; when associated with C-524. Loss of autophosphorylation; when associated with C-440 or C-440 and C-524.
Can form a disulfide bond; when associated with C-537. Loss of autophosphorylation; when associated with C-524 or C-524 and C-537.
Decreased autophosphorylation when CO- or NO-bound, none in the deoxy or O(2)-bound state, protein more easily oxidized to Fe(3+) state.
Isolated kinase core no longer forms dimers, autophosphorylation unaffected. Decreased autophosphorylation; when associated with C-537. Loss of autophosphorylation; when associated with C-440 or C-440 and C-537.
No autophosphorylation activity no matter the bound gaseous ligand, protein more easily oxidized to Fe(3+) state.
No autophosphorylation activity no matter the bound gaseous ligand.
No autophosphorylation when Fe(2+) protein is bound to CO or NO; no change in autophosphorylation of deoxy-protein. No change in auto-oxidation, slightly higher affinity for O(2) and CO.
No autophosphorylation, no transfer to DevR (DosR).
No autophosphorylation. Isolated kinase core binds ATP.
No change in autophosphorylation when NO-bound, decreased autophosphorylation in deoxy or CO-bound state, slightly increased activity in O(2)-bound state.
No change in autophosphorylation when NO-bound, loss of autophosphorylation in deoxy or CO-bound state.
No change in autophosphorylation when deoxy or NO-bound, decreased autophosphorylation when CO-bound, increased activity in O(2)-bound state.
Oxygen sensor histidine kinase response regulator DevS/DosS
Uncharacterized PPE family protein PPE23
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0145
Nicotinate phosphoribosyltransferase pncB1
In S11; may confer PZA resistance; when associated with R-21.
In S11; may confer PZA resistance; when associated with V-49.
Uncharacterized PE-PGRS family protein PE_PGRS10
Putative diacyglycerol O-acyltransferase Rv2484c
Reduces ATP-binding; M.tuberculosis overexpressing this protein grows normally.
Probable ECF RNA polymerase sigma factor SigI
Putative coenzyme F420-dependent oxidoreductase Rv3520c
PGL/p-HBAD biosynthesis rhamnosyltransferase
100-fold decrease in phosphopantetheinylation activity.
20-fold decrease in phosphopantetheinylation activity.
500-fold decrease in phosphopantetheinylation activity.
Abolishes phosphopantetheinylation activity.
Confers high-level resistance to compound 8918.
Schiff-base intermediate with acetaldehyde
Absence of EspD secretion. Decreases EspA levels.
Absence of EsxA secretion. Decreases EspD stability.
Decreased EspD secretion and small reduction in cellular EspA levels.
3-methyl-2-oxobutanoate dehydrogenase subunit beta
Uncharacterized PPE family protein PPE54
Phenolphthiocerol/phthiocerol polyketide synthase subunit D
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase