3-isopropylmalate dehydratase small subunit
Putative gamma-glutamylcyclotransferase PH0828
Resolvase/invertase-type recombinase catalytic
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
tRNA (pseudouridine(54)-N(1))-methyltransferase
Phosphoribosylformylglycinamidine synthase subunit PurL
Probable threonylcarbamoyladenosine tRNA methylthiotransferase
Signal recognition particle 54 kDa protein
Translation initiation factor 2 subunit gamma
Probable glycine dehydrogenase (decarboxylating) subunit 2
Uncharacterized HTH-type transcriptional regulator PH1916
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Probable ABC transporter permease protein PH1036
Signal recognition particle 19 kDa protein
UDP-N-acetyl-D-mannosamine dehydrogenase
O-(3'-phospho-DNA)-tyrosine intermediate
Probable ribosomal RNA small subunit methyltransferase A
Digeranylgeranylglyceryl phosphate synthase
Digeranylgeranylglycerophospholipid reductase
Non-specific cleavage pre-16S-rRNA, maybe due to altered substrate-binding.
Decreased dependence of aspartate binding on Na(+) concentration.
Increased rate of aspartate transport; when associated with R-395.
Increased rate of aspartate transport; when associated with S-276.
Strongly decreased affinity for aspartate.
Strongly decreased chloride conductance.
Putative methylthioribose-1-phosphate isomerase
Putative ABC transporter ATP-binding protein PH1815
Does not affect catalytic activity, but abolishes absorption at 300-500 nm.
Glutamyl-tRNA(Gln) amidotransferase subunit D
Putative 6-carboxy-5,6,7,8-tetrahydropterin synthase
Uncharacterized HTH-type transcriptional regulator PH1013
Probable chemoreceptor glutamine deamidase CheD
Phosphoribosylformylglycinamidine cyclo-ligase
GMP synthase [glutamine-hydrolyzing] subunit B
DNA-directed RNA polymerase subunit Rpo12
Significant deacylation of correctly charged L-alanyl-tRNA(Ala) occurs.
Dolichyl-phosphooligosaccharide-protein glycotransferase 2
Pyruvate/ketoisovalerate oxidoreductases common subunit gamma
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
Probable glycine cleavage system H protein
Translation initiation factor 2 subunit beta
Probable glycine dehydrogenase (decarboxylating) subunit 1
Uncharacterized HTH-type transcriptional regulator PH1692
Putative 1-aminocyclopropane-1-carboxylate deaminase
Putative transcriptional regulatory protein PH0763
Removed in mature form; by autocatalysis
Probable Brix domain-containing ribosomal biogenesis protein
Putative [LysW]-lysine/[LysW]-ornithine hydrolase
About 65% GTPase activity; when associated with 243-Q--Q-250. About 35% GTPase activity; when associated with 217-Q--Q-224.
About 65% GTPase activity; when associated with 272-Q--Q-279. About 35% GTPase activity; when associated with 217-Q--Q-224.
Binds less L12, about 50% GTPase activity, about 35% GTPase activity; when associated with 243-Q--Q250 or 272-Q--Q-279.
Nicotinamide-nucleotide adenylyltransferase
3-isopropylmalate dehydratase large subunit
Molybdate/tungstate-binding protein WtpA
Ribosomal RNA small subunit methyltransferase Nep1
tRNA (uracil(54)-C(5))-methyltransferase
2-fold decrease in the catalytic efficiency and strong decrease in the binding affinity.
Almost the same catalytic efficiency and binding affinity than wild-type.
Strong decrease in the catalytic efficiency and increase in the binding affinity.
DNA-directed RNA polymerase subunit Rpo6
120-fold decrease in catalytic efficiency.
4000-fold decrease in catalytic efficiency.
Almost complete loss of enzymatic activity.
Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Geranylgeranylglyceryl phosphate synthase
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2
3-methyl-2-oxobutanoate hydroxymethyltransferase
Indolepyruvate oxidoreductase subunit IorB
Adenosylcobinamide-GDP ribazoletransferase
Putative CRISPR-associated endoribonuclease-like protein Cas6nc
DNA double-strand break repair Rad50 ATPase
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
Trk system potassium uptake protein TrkA homolog
Ribosome maturation protein SDO1 homolog
Involved in ionization of N3 of dUMP, leading to its activation
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Uncharacterized ZPR1-like protein PH1223
DNA-directed RNA polymerase subunit Rpo11
Aspartate carbamoyltransferase regulatory chain
Probable molybdenum cofactor guanylyltransferase
Uncharacterized ABC transporter extracellular-binding protein PH1214
Glyceraldehyde-3-phosphate dehydrogenase
Schiff-base intermediate with dihydroxyacetone-P
Cysteine sulfenic acid (-SOH) intermediate
Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
Uncharacterized HTH-type transcriptional regulator PH0045
Pyridoxal 5'-phosphate synthase subunit PdxT
tRNA (cytidine(56)-2'-O)-methyltransferase
Phenylalanine--tRNA ligase alpha subunit
DNA-directed RNA polymerase subunit Rpo1C
tRNA-5-methyluridine(54) 2-sulfurtransferase
Putative HTH-type transcriptional regulatory protein PH1808
Does not reconstitute RNase P activity. Protein destabilized.
DNA repair and recombination protein RadB
Formate-dependent phosphoribosylglycinamide formyltransferase
Ketoisovalerate oxidoreductase subunit VorB
Tungsten-containing aldehyde ferredoxin oxidoreductase
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Ketoisovalerate oxidoreductase subunit VorD
tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS
O-(5'-phospho-DNA)-tyrosine intermediate
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Putative translation initiation factor eIF-2B subunit 2-like
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
Uncharacterized HTH-type transcriptional regulator PH1055
2-(3-amino-3-carboxypropyl)histidine synthase
Still able to bind a 4Fe-4S cluster. Less active than wild-type.
Still able to bind a 4Fe-4S cluster. Less active than wild-type. Not able to bind a 4Fe-4S cluster and loss of catalytic activity; when associated with Ala-287.
Still able to bind a 4Fe-4S cluster. Less active than wild-type. Not able to bind a 4Fe-4S cluster and loss of catalytic activity; when associated with Ala-59.
Uncharacterized HTH-type transcriptional regulator PH0061
Indolepyruvate oxidoreductase subunit IorA
DNA-directed RNA polymerase subunit Rpo5
Ketoisovalerate oxidoreductase subunit VorA
UPF0173 metal-dependent hydrolase PH1671
tRNA N6-adenosine threonylcarbamoyltransferase
Uncharacterized ATP-binding protein PH0541
Probable ABC transporter permease protein PH1216
Probable ABC transporter permease protein PH1038
Molybdate/tungstate import ATP-binding protein WtpC
Putative ABC transporter ATP-binding protein PH0132
EMO_00068 proton donor,EMO_00054 nucleophile,EMO_00058 nucleofuge,EMO_00066 proton acceptor
EMO_00068 proton donor,EMO_00066 proton acceptor,EMO_00066 proton acceptor,EMO_00068 proton donor
Probable S-adenosyl-L-methionine-binding protein PH1056
Phosphoribosylformylglycinamidine synthase subunit PurQ
Does not affect ability to stimulate RtcB ligase activity but has an effect on ATP utilization by RtcB.
Impaired ability to stimulate RtcB ligase activity.
Probable cobalamin biosynthesis protein CobD
DNA-directed RNA polymerase subunit Rpo10
Probable ABC transporter permease protein PH1215
Methylated-DNA--protein-cysteine methyltransferase
Translation initiation factor 2 subunit alpha
Protein-glutamate methylesterase/protein-glutamine glutaminase
DNA polymerase II large subunit, 1st part
DNA polymerase II large subunit, 2nd part
DNA double-strand break repair protein Mre11
Probable cyclic pyranopterin monophosphate synthase