Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-68 and E-71.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-68 and E-73.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-66; E-71 and E-73.
Strong decrease of the sporulation rate and loss of lipid binding; when associated with E-68; E-71 and E-73.
Strong decrease of the sporulation rate.
Hydroxyacyl-thioester dehydratase type 2, mitochondrial
Probable ADP-ribose 1''-phosphate phosphatase YML087W
Uncharacterized membrane protein YGR026W
General transcription and DNA repair factor IIH subunit TFB5
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
In SGC1-1; suppresses transcriptional defect caused by a GCR1 null mutation.
In SGC1-2/3/4; suppresses transcriptional defect caused by a GCR1 null mutation.
Abolishes inositol polyphosphate binding; when associated with A-127 and A-130.
Abolishes inositol polyphosphate binding; when associated with A-127 and A-134.
Abolishes inositol polyphosphate binding; when associated with A-130 and A-134.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with A-26 and A-131.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with F-22 and A-131.
Decreases affinity for inositol polyphosphate. Strongly decreases affinity for inositol polyphosphate; when associated with F-22 and A-26.
Vacuolar transporter chaperone complex subunit 2
For protease activity; shared with dimeric partner
Probable electron transfer flavoprotein subunit alpha, mitochondrial
In IPL1-1; causes missegregation of chromosomes at 37 degrees Celsius.
In IPL1-2; causes missegregation of chromosomes at 37 degrees Celsius.
In IPL1-4; causes missegregation of chromosomes at 37 degrees Celsius.
Ubiquitin carboxyl-terminal hydrolase YUH1
Defect in promoter association and change in chromatin structure.
Formamide-, hydroxyurea and UV-hypersensitivity, destabilization of ARP4, suppressor of TY phenotype and elevated levels of MSN2/MSN4-regulated genes. Lethal; when associated with A-23.
Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with S-23.
Lethal; when associated with D-161. Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with A-159.
No histone acetyltransferase activity at 37 degrees Celsius.
No histone acetyltransferase activity at 37 degrees Celsius. Defect in promoter association and change in chromatin structure.
Mitochondrial MYO2 receptor-related protein 1
Regulator of rDNA transcription protein 14
In DOC1-1; G2/M cell cycle arrest at 37 degrees Celsius.
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Leads to increased stability and reduced response to degradation signals.
Leads to normal response to the FPP-derived signal but no response to the oxysterol signal.
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 1, mitochondrial
tRNA pseudouridine(32) synthase, mitochondrial
Dramatically reduces interaction with RGA1.
In CDC42-1; temperature-sensitive mutant.
Guanine nucleotide exchange factor subunit RGP1
Uncharacterized plasma membrane protein YNL194C
Regulator of rDNA transcription protein 6
ISWI chromatin-remodeling complex ATPase ISW1
High-affinity potassium transport protein
In SEC14(ts); temperature-sesnitive allele that is targeted to the proteasome at the restrictive temperature.
Inactivates phosphatidylinositol, but not phosphatidylcholine, transfer activity, but rescues the lethality and Golgi secretory defects associated with sec14 null mutations; when associated with A-239.
Inactivates phosphatidylinositol, but not phosphatidylcholine, transfer activity, but rescues the lethality and Golgi secretory defects associated with sec14 null mutations; when associated with A-66.
Increased recombination centers protein 23
Vacuolar protein sorting-associated protein 41
Y' element ATP-dependent helicase protein 1 copy 1
Y' element ATP-dependent helicase protein 1 copy 5
Y' element ATP-dependent helicase protein 1 copy 8
Ubiquitin carboxyl-terminal hydrolase 16
Putative protein lysine methyltransferase SET5
Cells accumulate 35S and 23S pre-rRNA precursors. Cells display defects in translation elongation resulting in decreased translational accuracy.
Constitutively active allele, bypass the tRNA binding-requirement for kinase activity.
Does not abolish derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells.
Fails to derepress of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells and does not inhibit autophosphorylation.
Increases tRNA binding and the constitutive kinase activity and induces derepression of GCN4 translation and amino acid biosynthetic genes in absence of amino acid starvation.
Increases the constitutive kinase activity and induces derepression of GCN4 translation and amino acid biosynthetic genes in absence of amino acid starvation.
Increases the constitutive kinase activity in absence of amino acid starvation.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation in amino acid-starved cells.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation. Restores eIF-2-alpha kinase activity and derepression of GCN4, but not autophosphorylation; when associated with D-594.
Inhibits autophosphorylation, eIF-2-alpha kinase activity and derepression of GCN4 translation. Restores eIF-2-alpha kinase activity and derepression of GCN4, but not autophosphorylation; when associated with R-598.
Inhibits binding to uncharged tRNAs, decreases eIF-2-alpha kinase activity and derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells; when associated with L-1119.
Inhibits binding to uncharged tRNAs, decreases eIF-2-alpha kinase activity and derepression of GCN4 translation and amino acid biosynthetic genes in amino acid-starved cells; when associated with L-1120.
Inhibits dimerization; when associated with A-1080 and A-1088.
Inhibits dimerization; when associated with A-1080 and A-1090.
Inhibits dimerization; when associated with A-1088 and A-1090.
Inhibits interaction with GCN1, eIF-2-alpha phosphorylation and fails to derepress GCN4 translation in amino acid-starved cells.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with I-1553 and I-1556.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with L-1552 and I-1553.
Reduces interaction with TIF11, Inhibits binding to uncharged tRNAs, reduces autophosphorylation, eIF-2-alpha kinase activity and ribosome association, but not dimerization; when associated with L-1552 and I-1556.
In PRP83-2; temperature-sensitive; blocks splicing of RPS17A after a 2 hour shift to the restrictive temperature of 37 degrees Celsius; spliceosome assembly arrested at the complex I stage.
Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-251.
Lethal and dominant cold-sensitive. Reduces nuclear migration in mitosis; when associated with A-254.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis. Alters complex assembly; when associated with A-219.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-266.
Lethal. Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-269.
Lethal. Strongly reduces interaction with JNM1.
Lethal. Strongly reduces interaction with JNM1; when associated with A-133.
Lethal. Strongly reduces interaction with JNM1; when associated with A-136.
Lethal. Strongly reduces interaction with JNM1; when associated with A-369.
Lethal. Strongly reduces interaction with JNM1; when associated with A-371.
Lethal; when associated with 214-A--A-216.
Reduces nuclear migration in mitosis; when associated with 368-A-A-369.
Reduces nuclear migration in mitosis; when associated with A-294 and A-296.
Reduces nuclear migration in mitosis; when associated with A-294 and A-298.
Reduces nuclear migration in mitosis; when associated with A-296 and A-298.
Reduces nuclear migration in mitosis; when associated with A-371.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with 84-A-A-85.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-162.
Reduces spindle formation. Reduces nuclear migration in mitosis; when associated with A-165.
Reduces spindle formation. Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-87.
Reduces spindle formation; when associated with A-73.
Reduces spindle formation; when associated with A-75.
Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with 48-A-A-49.
Strongly reduces interaction with JNM1. Reduces nuclear migration in mitosis; when associated with A-46.
Strongly reduces interaction with JNM1; when associated with A-336.
Strongly reduces interaction with JNM1; when associated with A-338.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with 374-A-A-375.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-326.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-328.
Temperature-sensitive. Reduces nuclear migration in mitosis. Strongly reduces interaction with JNM1; when associated with A-378.
Temperature-sensitive. Strongly reduces interaction with JNM1.
Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-185.
Temperature-sensitive. Strongly reduces interaction with JNM1; when associated with A-187.
Temperature-sensitive; when associated with A-2.
Temperature-sensitive; when associated with A-6.
ABC transporter ATP-binding protein/permease VMR1
Probable alanine aminotransferase, mitochondrial
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-21 and A-60.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-149 and A-260.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-149 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-135; A-260 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-60; A-83; A-149; A-260 and A-475.
Abolishes activity; when associated with A-20; A-21; A-58; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-21 and A-83.
Abolishes activity; when associated with A-20; A-21; A-60; A-83; A-135; A-149; A-260 and A-475.
Abolishes activity; when associated with A-20; A-58; A-60; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-20; A-60 and A-83.
Abolishes activity; when associated with A-21; A-58; A-60; A-83; A-135; A-149; A-260 and A-475. Abolishes activation by BMH1 and BMH2; when associated with A-21; A-60 and A-83.
Transcription and mRNA export factor SUS1
No acetylation, reduced silencing activity.
Asparagine-rich zinc finger protein AZF1
Mitochondrial distribution and morphology protein 36
Circumvents the requirement of the CTD to enhance substrate trans-location through the hydrophobic core.
Decreases the affinity for the substrate.
Leads to increased competence to acidify the submembrane pH while losing the signaling capability.
Preserves transport activity while impairing the filamentation capacity.
Silences the autoinhibition of MEP2, conferring transport activity even in the absence of the NPR1 kinase.
CRAL-TRIO domain-containing protein YKL091C
Vacuolar protein sorting-associated protein 38
Cytochrome c oxidase subunit 5A, mitochondrial
Regulator of V-ATPase in vacuolar membrane protein 2
Cytochrome c oxidase assembly protein COX19
In COX19-1; causes a respiratory-deficient phenotype because of lack of cytochrome c activity.
Cell cycle serine/threonine-protein kinase CDC5/MSD2
37S ribosomal protein RSM18, mitochondrial
H/ACA ribonucleoprotein complex subunit NHP2
Lethal. Impaired association with H/ACA snoRNAs. Accumulation of NHP2 within the nucleolus.
Significant growth impairment at 30 and 37 degrees Celsius. Impaired association with H/ACA snoRNAs.
Translation machinery-associated protein 16
Cytochrome c oxidase subunit 8, mitochondrial
Putative tyrosine-protein phosphatase OCA1
ATP-dependent permease MDL2, mitochondrial
37S ribosomal protein MRP17, mitochondrial
Antagonist of mitotic exit network protein 1
Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin.
Prevents the release of AFG2, RLP24 and NOG1 from pre-60S ribosomal particles. Enhances growth defect in an AFG2 (drg1-18) mutant background.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-40.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with D-44.
Vacuolar protein sorting-associated protein 28
Low-affinity phosphate transporter PHO91
Mimics acetylation; does not show increased sensitivity to DNA damaging agents.
Mimics glutarylation; delays in cell proliferation; increased sensitivity to DNA damaging agents.
Mimics unmodified residue; does not show increased sensitivity to DNA damaging agents.
Negative cofactor 2 complex subunit beta
Pyruvate dehydrogenase complex protein X component, mitochondrial
Appears to have no effect on the ATPase activity of membrane-detached V-ATPase V1 or on cell population growth.
Increases the ATPase activity of membrane-detached V-ATPase V1, appears to have no effect on cell population growth.
Nascent polypeptide-associated complex subunit beta-2
Helical; Signal-anchor for type II membrane protein
In strain: YPH499; lack of gamma-glutamyl transferase activity.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
RNA polymerase I-specific transcription initiation factor RRN5
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Altered inheritance of mitochondria protein 20
Uncharacterized protein YDL157C, mitochondrial
Putative uncharacterized protein YHR050W-A
Putative 2-hydroxyacid dehydrogenase YPL113C
Chromatin structure-remodeling complex subunit RSC9
In TSC13-1; reduces fatty acid elongation activity by 50%.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and A-261.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; N-66 and R-162.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with E-20; R-162 and A-261.
In temperature-sensitive mutant KRR1-17; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with N-66; R-162 and A-261.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and G-207.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with L-45 and S-95.
In temperature-sensitive mutant KRR1-18; grows normally at 25 degrees Celsius but fails to grow at 35 degrees Celsius; when associated with S-95 and G-207.
Vacuolar protein sorting-associated protein 73
In allele CUS1-54; cold-sensitive growth suppressor.
ATP-dependent RNA helicase MSS116, mitochondrial
2-hydroxy-palmitic acid dioxygenase MPO1
Putative uncharacterized protein YHR045W
Functional kinase, that can be rapidly inhibited by small, cell-permeable drugs like 1-Na PP1 (4-amino-1-tert-butyl-3-(1'-naphthyl)pyrazolo[3,4-d]pyrimidine).
Loss of kinase activity. Abolishes interaction to PHO80 and PCL1 cyclins.
Reduces kinase activity. Abolishes interaction to PHO80 cyclin, but not to PCL1.
In SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-483. In SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with 477-AA-478.
In SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-487.
In SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with A-487.
PXIXIT-like, required for interaction with CNA1 and CNA2, and calcineurin-dependent dephosphorylation
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1
Decreases catalytic activity for L-tyrosine 400-fold, but allows the utilization of 3-iodo-L-tyrosine and other 3-modified tyrosines as substrates.
Putative mitochondrial translation system component PET127
Vacuolar import and degradation protein 28
Impairs maturation and amino acid-induced STP1 cleavage.
In SSY5-13; constitutively active, confers 9.3% increased STP1 processing in the absence of amino acids.
In SSY5-14; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
In SSY5-15; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
In SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino acids; when associated with H-632.
In SSY5-18; constitutively active, confers 15.5% increased STP1 processing in the absence of amino acids; when associated with N-581.
In SSY5-6; constitutively active, confers 30% increased STP1 processing in the absence of amino acids.
Prevents maturation and amino-acid-induced STP1 cleavage.
SPS-sensor serine protease component SSY5
Growth inhibited above 30 degrees Celsius.
Growth inhibited above 34 degrees Celsius.
Leads to mislocalization of NPCs and overproliferation of the nuclear and ER membranes at 34 degree Celsius.
Abolishes GTP-binding; no strong effect in vivo.
Loss of function; abolishes association with PI(4)P and PI(5)P.
Loss of function; strongly decreases association with PI(4)P and PI(5)P.
Temperature-sensitive; no effect at 23 degrees Celsius but loss of function at 37 degrees Celsius.
Temperature-sensitive; no effect at 23 degrees Celsius but loss of function at 37 degrees Celsius; abolishes interaction with itself.
In PCF1-11; increases RNA polymerase III gene transcription.
In PCF1-12; increases RNA polymerase III gene transcription.
In PCF1-138; increases RNA polymerase III gene transcription.
In PCF1-139; increases RNA polymerase III gene transcription.
In PCF1-15; increases RNA polymerase III gene transcription.
In PCF1-17; increases RNA polymerase III gene transcription.
In PCF1-19; increases RNA polymerase III gene transcription.
In PCF1-1; affects the rate of recruitment of TFIIIB to the template. Increases the amount of transcriptionally active TFIIIB. Increases RNA polymerase III gene transcription. Increases the binding affinity for BRF1, but does not affect the binding affinity for BDP1 in the TFIIIC-dependent assembly of TFIIIB. Overcomes autoinhibition of BRF1 binding.
In PCF1-23; increases RNA polymerase III gene transcription.
In PCF1-2; increases RNA polymerase III gene transcription due to an increase in the recruitment of BRF1 to TFIIIC-DNA. No effect on affinity of TFIIIC for DNA.
In PCF1-3; increases RNA polymerase III transcription two- to threefold over wild-type. Increases the amount of transcriptionally active TFIIIB.
In PCF1-4; increases RNA polymerase III transcription ninefold over wild-type. Increases the amount of transcriptionally active TFIIIB.
In PCF1-5; increases RNA polymerase III transcription.
In PCF1-6; increases RNA polymerase III transcription.
In PCF1-7; increases RNA polymerase III transcription.
In PCF1-8; increases RNA polymerase III transcription.
RNA polymerase III defective. Defect in the recruitment of BRF1 into TFIIIB-TFIIIC-DNA complexes and diminished direct interaction between TFC4 and BRF1. Decreased binding affinity for BDP1 incorporation into TFIIIB-TFIIIC-DNA complexes and inhibited binary interaction between BDP1 and TFC4.
Transcription factor tau 131 kDa subunit
Rab proteins geranylgeranyltransferase component A
Resistance to glucose repression protein 1
54S ribosomal protein L39, mitochondrial
Leads to a defect in complex IV assembly and subsequent respiratory deficiency.
Leads to a defect in complex V assembly and subsequent respiratory deficiency.
Leads to a defect in complexes IV and V assembly, and subsequent respiratory deficiency.
Mitochondrial inner membrane protein OXA1
Leads to complete loss of association with ub-hotspots; when associated with A-33.
Leads to complete loss of association with ub-hotspots; when associated with A-34.
Strongly reduces EUC1 sumoylation and the formation of ub-hotspots.
UDP-N-acetylglucosamine transferase subunit ALG14
Completely abolishes reductase activity.
Ribonucleoside-diphosphate reductase large chain 1
Vacuolar protein sorting-associated protein 71
Further reduces interaction with eIF4E/TIF45; when associated with A-4.
Reduces interaction with eIF4E/TIF45. Prevents pseudohyphal growth. Further reduces interaction with eIF4E/TIF45; when associated with A-9.
DNA-directed RNA polymerases I and III subunit RPAC1
Leads to the loss of catalytic activity.
Thiosulfate:glutathione sulfurtransferase
In strain: SCI3, SCI4, SCI7.2/a, SCI7.2/b, SCI7.2/d, SCI11.5/a, SCI11.5/b, SCI11.5/c and SCI11.5/d.
In strain: SCI7.2/a, SCI7.2/d, SCI11.5/b and SCI11.5/d.
In strain: SCI7.2/b, SCI11.5/a and SCI11.5/c.
In strain: SCI7.2/b, SCI7.2/d and SCI11.5/c.
In strain: SCI7.2/b,SCI7.2/d and SCI11.5/c.
54S ribosomal protein L37, mitochondrial
Helical; Anchor for type IV membrane protein
Sensitive in DMS protection of U1 snRNA.
Sensitive in DMS protection of U1 snRNA; when associated with 6-I-N-7.
Sensitive in DMS protection of U1 snRNA; when associated with 65-D--L-67.
Sensitive in DMS protection of U1 snRNA; when associated with 74-R-G-75.
Sensitive in DMS protection of U1 snRNA; when associated with 79-V-I-80.
Sensitive in DMS protection of U1 snRNA; when associated with I-4.
Sensitive in DMS protection of U1 snRNA; when associated with I-63.
Splicing defects; when associated with 230-F--T-232.
Splicing defects; when associated with 262-D-I-263.
Splicing defects; when associated with 291-A--K-293.
Splicing defects; when associated with F-268.
Splicing defects; when associated with I-228.
Splicing defects; when associated with S-295.
Monoisotopic mass with either 7 methylation modifications or 1 acetylation and 4 methylation modifications.
DNA repair and recombination protein RAD26
Putative uncharacterized protein YLL053C
Vacuolar protein sorting-associated protein 55
DNA-directed RNA polymerase III subunit RPC9
Putative uncharacterized protein YLR125W
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Completely abolishes catalytic activity.
Has weaker affinity for S-adenosyl-L-methionine and reduces catalytic activity by 90%.
Mediator of RNA polymerase II transcription subunit 12
Isocitrate dehydrogenase [NADP] cytoplasmic
Abolishes interaction with ORC1 and SIR4.
Mitochondrial import receptor subunit TOM40
Cytochrome B pre-mRNA-processing protein 1
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the 60S subunit; when associated with E-95.
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the 60S subunit; when associated with E-98.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-289 and A-290.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-289 and A-295.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-290 and A-295.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with A-334.
Leads to an inefficient release from ACL4 with a delayed assembly into the 60S subunit; when associated with e-332.
Significantly diminished nuclear localization; when associated with A-314 and A-315.
Significantly diminished nuclear localization; when associated with A-314 and A-319.
Significantly diminished nuclear localization; when associated with A-315 and A-319.
54S ribosomal protein L34, mitochondrial
54S ribosomal protein L38, mitochondrial
Decreases transport activity and GDP-mannose-binding.
In VIG4-1; increases drug sensitivity and decreases protein glycolysis.
In VIG4-2; increases drug sensitivity and decreases protein glycolysis.
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1
Abolishes pseudouridylate synthase activity.
Decreased pseudouridylate synthase activity.
Does not affect pseudouridylate synthase activity.
Strongly decreased pseudouridylate synthase activity.
Probable serine/threonine-protein kinase RTK1
Strongly reduces UBC13 binding and interferes with error-free DNA repair.
Ubiquitin-conjugating enzyme variant MMS2
DNA-directed RNA polymerase II subunit RPB11
Transcript termination readthrough. Lethal.
Proton acceptor; for methoxycarbonylation activity
Proton donor; for both methylation and methoxycarbonylation activities
Vacuolar protein sorting-associated protein 51
37S ribosomal protein S23, mitochondrial
In PRP5-1; no growth at 37 degrees Celsius and impairs pre-spliceosome formation in vitro.
Pre-mRNA-processing ATP-dependent RNA helicase PRP5
Actin-related protein 2/3 complex subunit 2
Uroporphyrinogen-III C-methyltransferase
Conserved oligomeric Golgi complex subunit 8
Chromatin structure-remodeling complex protein RSC14
Cytoplasmic 60S subunit biogenesis factor REH1
Altered inheritance of mitochondria protein 46, mitochondrial
Glutathione-independent glyoxalase HSP31
PAN2-PAN3 deadenylation complex subunit PAN3
Significantly decreases interaction with PAN1.
Uncharacterized transcriptional regulatory protein TBS1
Morphogenetic regulator of filamentous growth protein 1
ERAD-associated E3 ubiquitin-protein ligase ASI1
In ASI1-21HA; when associated with S-583; abolishes function.
In ASI1-21HA; when associated with S-585; abolishes function.
Putative ATP-dependent RNA helicase ECM32
Auxilin-like clathrin uncoating factor SWA2
In SWA2-1/SWA2-TPR; partial loss of clathrin disassembly function. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with 631-AAA-633.
In SWA2-J; abolishes ATPase stimulation activity. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with R-388.
Probable pyridoxal 5'-phosphate synthase subunit SNO2
Mitochondrial import receptor subunit TOM20
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5
Attenuates the coordination of ion and is unable to sustain growth in thiamine-free medium.
CCCFC; essential for catalytic activity, may be the site of iron coordination
This mass is that of the untagged protein plus the mass of an iron ion.
Unable to sustain growth in thiamine-free medium.
No ATPase activity; increase in chromosome missegregation.
NEDD8-activating enzyme E1 catalytic subunit
Causes dissociation of the homotetramer to dimers at low concentrations.
Reduces kcat of the mutase reaction 10000-fold.
Branched-chain-amino-acid aminotransferase, cytosolic
Putative aryl-alcohol dehydrogenase AAD10
Calcium permeable stress-gated cation channel 1
Anaphase-promoting complex subunit CDC26
Tethering factor for nuclear proteasome STS1
Decreases substrate-binding and reduces mannosyltransferase activity.
Dolichyl-phosphate-mannose--protein mannosyltransferase 1
Moderately decreases complex formation with PMT2.
Abolishes cleavage of substrate oligonucleotides.
Cannot properly relocalize in response to oxidative stress.
In NTG1(mts); reduces mitochondrial localization by 40%.
In NTG1(nls1); reduces nuclear localization by 60%.
In NTG1(nls2); reduces nuclear localization by 60%.
Pleiotropic ABC efflux transporter of multiple drugs
Altered inheritance rate of mitochondria protein 25
Transcription initiation factor IIE subunit beta
Slightly increases affinity for poly-U RNA ends.
U6 snRNA-associated Sm-like protein LSm5
Uncharacterized membrane glycoprotein YNR066C
Impairs sumoylation; when associated with R-201.
Impairs sumoylation; when associated with R-215.
tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
DNA-directed RNA polymerase II subunit RPB1
Impairs ubiquitination during transcription stress.
Abolishes interaction with eIF4E; when associated with A-109.
Abolishes interaction with eIF4E; when associated with A-114.
In strain: A2-M8; confers resistance to the allylamine antifungal terbinafine.
In cdc68-1; induces a spt phenotype characterized by depletion of many mRNAs.
In spt16-4; induces a spt phenotype characterized by depletion of many mRNAs; when associated with L-570.
In spt16-4; induces a spt phenotype characterized by depletion of many mRNAs; when associated with S-565.
In spt16-6; induces a spt phenotype characterized by depletion of many mRNAs.
In spt16-7; induces a spt phenotype characterized by depletion of many mRNAs.
Eukaryotic translation initiation factor 4B
Signal recognition particle subunit SRP72
Reduces the interaction with POL30; when associated with A-42 or D-42.
General transcription and DNA repair factor IIH subunit TFB2
Target of rapamycin complex 2 subunit TSC11
In PTF1-2; decreases the catalytic efficiency 20-fold.
In PTF1-5; decreases the catalytic efficiency 12-fold.
Peptidyl-prolyl cis-trans isomerase ESS1
3',5'-cyclic-nucleotide phosphodiesterase 1
3',5'-cyclic-nucleotide phosphodiesterase 2
No effect on MCM2-7 complex helicase activity. Loss of MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM2 A-549.
Helical; Signal-anchor for type III membrane protein
Complete loss of ability to support homology search.
DNA repair and recombination protein RAD54
Impairs association with the nuclear pores and interaction with MTR2.
Chromosome segregation in meiosis protein 3
DNA repair and recombination protein RAD52
25S rRNA (uridine(2634)-N(3))-methyltransferase
DNA-directed RNA polymerase III subunit RPC8
Vacuolar protein sorting-associated protein 52
Decreases ability to interact with TAF11 and support growth on galactose-containing medium. Unable to support cell viability in a strain deleted for TOA2; when associated with A-69.
Decreases ability to interact with Toa1 and TAF11, display mutant growth phenotypes and defects in transcription in vivo.
Transcription initiation factor IIA subunit 2
Unable to support cell viability in a strain deleted for TOA2; when associated with A-27 or K-27.
Induces dysregulation of sphingolipid synthesis; when associated with 32-A--A-36 and 51-A--A-53.
Induces dysregulation of sphingolipid synthesis; when associated with A-29 and 32-A--A-36.
Induces dysregulation of sphingolipid synthesis; when associated with A-29 and 51-A--A-53.
Abolishes interaction with PCNA (POL30), but only causes a moderate mismatch repair defect. Partially functional in a mismatch repair assay; when associated with 26-AA-27.
Defective in repairing 8-oxo-G-A mismatches.
Fully functional in a mismatch repair assay.
Has no defect in mismatch DNA binding, but lacks ATP-induced conformational change.
Impairs ATP binding; reduces catalytic activity 13-fold for ATP hydrolysis.
In MSH6-2; defective for ATP-induced sliding clamp formation, but assembles ternary complexes with MutL alpha.
In MSH6-340; shows defects in mispair DNA binding, but not in homoduplex DNA binding.
In MSH6-3; defective for ATP-induced sliding clamp formation and assembly of ternary complexes with MutL alpha.
In MSH6-4; defective for ATP-induced sliding clamp formation and assembly of ternary complexes with MutL alpha.
In MSH6-5; partially functional in a mismatch repair assay.
In MSH6-6; partially functional in a mismatch repair assay.
In MSH6-7; partially functional in a mismatch repair assay.
In MSH6-9; shows normal mispair binding and dissociation, but fails to show complete mispair activation of the ATPase.
In PMS3-1; completely abolishes mismatch repair.
Moderately reduced activity in a mismatch repair assay.
Partially functional in a mismatch repair assay.
Partially functional in a mismatch repair assay; when associated with 33-AA-34.
Reduces catalytic activity 13-fold for ATP hydrolysis.
Shows defects in both homoduplex and mispair DNA binding and is only partially functional in a mismatch repair assay.
Probable alpha-1,3-mannosyltransferase MNT4
NADP-dependent 3-hydroxy acid dehydrogenase
Uncharacterized protein YML007C-A, mitochondrial
Abolishes dimer formation. Decreases activity and binding to RTT109.
Decreases RTT109 binding and activity stimulation.
Increases acetylation of histone H3 'Lys-56'.
Mildly decreases RTT109 activity stimulation.
Vacuolar protein sorting-associated protein 75
Putative endoplasmic reticulum mannosidase MNL2
Cytosolic Fe-S cluster assembly factor CFD1
Loss of function and disrupts heterotetramer formation.
Uncharacterized membrane protein YFL067W
U3 small nucleolar RNA-associated protein 22
In cs mutant CDC44-2; causes cell cycle arrest.
In cs mutant CDC44-3/4; causes cell cycle arrest.
In cs mutants CDC44-1/5/8 and CDC44-9; causes cell cycle arrest.
Loss of chelatase and dehydrogenase activity.
Loss of dehydrogenase activity. No effect on chelatase activity.
No effect on dehydrogenase or chelatase activity.
Pyridoxal 5'-phosphate synthase subunit SNO1
Most catalytic properties similar to wild-type.
Reduced affinity for xylulose 5-phosphate and strongly reduced affinity for ribose 5-phosphate.
Reduces activity by over 96% and decreases affinity for thiamine pyrophosphate and xylulose 5-phosphate.
Reduces catalytic activity by about 95%.
Reduces catalytic activity by about 98%. Decreased affinity for donor substrate.
Slightly reduced affinity for xylulose 5-phosphate and strongly reduced affinity for ribose 5-phosphate.
Strongly reduced affinity for xylulose 5-phosphate and ribose 5-phosphate.
Strongly reduced catalytic activity. Decreases affinity for xylulose 5-phosphate about 15 times.
Strongly reduced catalytic activity. Strongly reduced affinity for xylulose 5-phosphate and ribose 5-phosphate.
Loss of function; when associated with S-112.
Loss of function; when associated with S-116.
RNA polymerase II subunit B1 CTD phosphatase RTR1
Decreases JAC1 ATPase activator activity. Decreases vegetative cell population growth. Sensitive to high temperature.
Decreases JAC1 ATPase activator activity. Decreases vegetative cell population growth. Sensitive to high temperature. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; S-38 and L-41.
Decreases JAC1 ATPase activator activity; when associated with A-20; A-35 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; L-41 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-20; A-35 and S-38. Abolishes JAC1 ATPase activator activity; when associated with A-20; A-35; S-38 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-20; S-38 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-20; S-38; L-41 and A-48.
Decreases JAC1 ATPase activator activity; when associated with A-35; S-38 and L-41. Abolishes JAC1 ATPase activator activity; when associated with A-35; S-38; L-41 and A-48.
In JAC1(LKDDEQ); impairs interaction with ISU1, but does not affect stimulation of SSQ1 ATPase activity.
In JAC1-1; temperature sensitive; causes growth defect at high temperatures.
In JAC1-A3; temperature sensitive; causes growth defect at high temperatures.
Mislocalizes to the vacuole membrane and stay associated with the V0 sector after transport out of the ER.
Vacuolar ATPase assembly integral membrane protein VMA21
Reduces catalytic activity towards homoaconitate by 45% and increases the activity towards aconitate by a factor 116.
Styryl dye vacuolar localization protein 3
U3 small nucleolar RNA-associated protein 11
Disrupts homotypic domain interactions; when associated with D-111 and D-113.
Disrupts homotypic domain interactions; when associated with D-111 and D-118.
Disrupts homotypic domain interactions; when associated with D-113 and D-118.
Disrupts homotypic domain interactions; when associated with D-133 and D-144.
Disrupts homotypic domain interactions; when associated with D-133 and D-196.
Disrupts homotypic domain interactions; when associated with D-144 and D-196.
Increased shedding, associated with loss of GPI-anchor site.
DNA polymerase alpha-associated DNA helicase A
Probable vacuolar amino acid transporter YPQ2
37S ribosomal protein S17, mitochondrial
In the cold-sensitive allele sen2-3; defective in cleavage only at the 5'-splice site of tRNA precursors.
No effect. Synthetic lethal when associated with CLF1 lacking a TPR domain.
In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
Serine/threonine-protein phosphatase 1 regulatory subunit GAC1
Loss of recruitment to vacuole membrane.
Asymmetric dimethylarginine; by HMT1; alternate
H/ACA ribonucleoprotein complex subunit GAR1
Omega-N-methylarginine; by HMT1; alternate
Zinc-regulated GTPase metalloprotein activator 1
Abolishes interaction with SSC1, and leads to self-aggregation of mtHSP70 and accumulation of uncleaved precursor proteins.
In TIM15-2RH; Abolishes interaction with SSC1, and leads to self-aggregation of mtHSP70 and accumulation of uncleaved precursor proteins.
Mitochondrial protein import protein ZIM17
Temperature sensitive; only partly prevents self-aggregation of mtHSP70 and leads to accumulation of uncleaved precursor proteins at high temperatures.
Asparagine synthetase domain-containing protein YML096W
Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly.
Mitochondrial respiratory chain complexes assembly protein AFG3
No cell growth at 23 or 37 degrees Celsius.
No cell growth at 23 or 37 degrees Celsius. Decreases GTP binding and impairs ER-to-Golgi vesicle transport.
No cell growth at 23 or 37 degrees Celsius. Impairs ER-to-Golgi vesicle transport.
No cell growth at 37 degrees Celsius. Decreases GTP binding and ER-to-Golgi vesicle transport.
Putative elongation factor 1 gamma homolog
Impairs plasma membrane ingression; when associated with A-28.
Impairs plasma membrane ingression; when associated with A-31.
In ZAP1-1up; interferes with the zinc-dependent repression of the ZRT1 promoter but does not prevent full induction of the ZRT1 gene.
Results in a significant defect in zinc responsiveness; when associated with A-202 and A-203.
Results in a significant defect in zinc responsiveness; when associated with A-202 and A-207.
Results in a significant defect in zinc responsiveness; when associated with A-203 and A-207.
Zinc-responsive transcriptional regulator ZAP1
25S rRNA (cytosine(2870)-C(5))-methyltransferase
Cannot complement loss of wild type NOP2.
Fails to ctalyze the C-5 methylation of the C2870 residue and strongly affects 60S biogenesis.
Maltose fermentation regulatory protein YPR196W
DNA-directed RNA polymerase II subunit RPB9
Uncharacterized membrane protein YJR124C
TATA-binding protein-associated factor MOT1
Suppresses the growth defects caused by mutations of flippases.
Phosphatidylinositol N-acetylglucosaminyltransferase ERI1 subunit
Uncharacterized membrane protein YGR016W
S-(hydroxymethyl)glutathione dehydrogenase
Glycine cleavage system H protein, mitochondrial
Probable glutathione-independent glyoxalase HSP32
Not palmitoylated, rapidly degraded in a TUL1-dependent manner.
Results in an aktivated t-SNARE that confers endocytosis, but not exocytosis.
t-SNARE affecting a late Golgi compartment protein 1
UPF0674 endoplasmic reticulum membrane protein YNR021W
Abolished pseudouridylation of rRNA. Abolishes pseudouridylation at position 93 in U2 snRNA.
H/ACA ribonucleoprotein complex subunit CBF5
Reduced pseudouridylation of rRNA and reduced snoRNA levels.
ATP synthase subunit gamma, mitochondrial
In ATP3-1; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA.
In ATP3-5; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA.
Inosine-5'-monophosphate dehydrogenase 4
Impairs cell growth and lowers aconitase activity.
Impairs cell growth, lowers aconitase activity and strongly decreases interaction with ISU1.
Impairs cell growth, lowers activity of mitochondrial iron-sulfur cluster-containing enzymes, no effect on iron binding and oligomerization.
Impairs oligomerization and iron mineralization.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-103.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-97.
Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-97 and A-103.
Lowers aconitase activity and no effexct on interaction with ISU1.
Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-79.
Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-82.
In strain: CLIB 219, CLIB 410, CLIB 413, CLIB 630 haplotype Ha1, K1, YIIc12 haplotype Ha2 and CLIB 382.
Locks VPS21 in its GDP-bound form by abolishing binding to GTP.
Vacuolar protein sorting-associated protein 21
tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial
NADPH-dependent diflavin oxidoreductase 1
Proton acceptor; for 3-dehydroquinate dehydratase activity
Proton acceptor; for 3-dehydroquinate synthase activity
Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity
ERAD-associated E3 ubiquitin-protein ligase HRD1
Stabilizes HRD1. Reduced interaction with substrate. Formation of oligomer with or without USA1.
Has defects on both initial ubiquitination and chain elongation of substrate proteins.
Has subtle defects on both initial ubiquitination and chain elongation of substrate proteins.
In RSP5-1; impairs ubiquitin-thioester formation and catalysis of substrate ubiquitination.
RNA polymerase I-specific transcription initiation factor RRN6
No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F and Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization.
No effect on growth kinetics nor on actin cytoskeleton morphology. Resistant to Chivosazole F inhibition of polymerization. Increases penetrance of Latrunculin A inhibition of polymerization. No effect on Chondramide inhibition of polymerization.
Reduced kinase activity; when associated with A-641.
Reduced kinase activity; when associated with A-659.
Rescues growth of cells compromised in TORC2, but not TORC1 function. Restores SLT2 activation and suppresses actin cytoskeleton organization defect in TOR2 mutant cells.
Serine/threonine-protein kinase YPK2/YKR2
CCR4-NOT transcriptional complex subunit CAF120
General control transcription factor GCN4
In strain: CLIB 388, CLIB 410, CLIB 413, CLIB 630 haplotype Ha1, K1, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1.
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13 haplotype Ha2, Sigma 1278B haplotype Ha1, YIIc12 and YIIc17.
Reduces transcriptional activation activity; when associated with A-107; A-110; A-113; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-110 and A-113.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-110; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-107; A-113; A-120; A-123 and A-124.
Reduces transcriptional activation activity; when associated with A-97; A-98; A-110; A-113; A-120; A-123 and A-124.
Cytochrome oxidase assembly protein SHY1
CCA tRNA nucleotidyltransferase, mitochondrial
Peroxisomal long-chain fatty acid import protein 2
Probable E3 ubiquitin-protein ligase HUL4
Ribonucleases P/MRP protein subunit POP1
Structural maintenance of chromosomes protein 2
RNA-binding protein RMD9-like, mitochondrial
Abolishes binding to N6-methyladenosine (m6A)-containing RNAs.
Dehydrodolichyl diphosphate synthase complex subunit RER2
Membrane-anchored lipid-binding protein YSP2
Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation.
Diminishes the formation of oligomers, dampens activation of the HOG1 kinase, and impairs growth in high-salt or sorbitol conditions.
Geranylgeranyl transferase type-2 subunit alpha
Cell wall integrity and stress response component 4
In IQG1-1; causes a defect in cytokinesis at 37 degrees Celsius.
6-phosphogluconolactonase-like protein 2
Protein phosphatase PP2A regulatory subunit B
Strongly reduces HAT activity at pH 9.2. Nearly abolishes HAT activity at pH 8.0, but is not lethal (in vivo). Lethal; when associated with S-334.
Strongly reduces HAT activity, but is not lethal (in vivo). Lethal, when associated with Q-338.
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI11
Abolishes aminopeptidase activity. No effect on the epoxide hydrolase activity.
Abolishes the aminopeptidase activity and decreases the epoxide hydrolase activity.
Abolishes the epoxide hydrolase activity.
Abolishes the epoxide hydrolase and aminopeptidase activities.
Increases the aminopeptidase activity and decreases the epoxide hydrolase activity.
Reduces strongly the substrate affinity.
Low residual phosphatase activity. Loss of stress response functions.
Trafficking protein particle complex II-specific subunit 130
Abolishes SKT5 prenylation. Decreases chitin synthase CHS3 activity. Decreases chitin chain length. Decreases cellular chitin level. Normal SKT5 localization to cellular membranes. Resistance to Calcofluor White (cell wall stressor).
Decreases chitin synthase CHS3 activity, resistance to Calcofluor White (cell wall stressor), decreases cellular chitin level.
Does not accumulate in the nucleus in response to cadmium.
Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-356.
Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-387.
Impaired heterodimerization with ARP7; when associated with L-338.
Impaired heterodimerization with ARP7; when associated with V-337.
Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85.
No phosphorylation by PKA, and strong decrease in activity; when associated with A-30.
Endoplasmic reticulum transmembrane protein 2
Decreases the association to PtdIns(3)P containing membranes.
Reduces strongly the ubiquitin-binding activity.
Vacuolar protein sorting-associated protein 27
Phenylalanine--tRNA ligase alpha subunit
Removed in mature form; by autocatalysis
Putative uncharacterized protein YLR156W
Putative uncharacterized protein YLR159W
Putative uncharacterized protein YLR161W
E3 ubiquitin-protein ligase linker protein MMS1
Abolished monoubiquitination by PEX2, promoting polyubiquitination by PEX10 and degradation. Loss of UBE2D2-independent ubiquitination. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with R-18 and R-24.
Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)
Loss of UBE2D2-dependent ubiquitination. No effect on its function. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with A-6 and R-18.
Loss of UBE2D2-dependent ubiquitination. No effect on its function. Abolished monoubiquitination by PEX2 and polyubiquitination by PEX10, leading to impaired protein import in peroxisomes; when associated with A-6 and R-24.
Pyridoxamine 5'-phosphate oxidase homolog
No defect in the interaction with CDC48.
Mannosyl phosphorylinositol ceramide synthase SUR1
Meiosis-specific APC/C activator protein AMA1
27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase
Transcription initiation factor TFIID subunit 5
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
Signal recognition particle receptor subunit alpha homolog
Abolishes endoribonucleolytic activity; no effect on growth. No growth; when associated with N-551.
Exoribonucleolytic activity abolished. Accumulation of partially processed 5.8S rRNA and partially degraded 5' ETS. No growth; when associated with A-171. No growth; when associated with A-198.
Slow growth; when associated with S-47 and S-52.
Slow growth; when associated with S-47 and S-55.
Slow growth; when associated with S-52 and S-55.
Extender of the chronological lifespan protein 1
Putative ATP-dependent RNA helicase YLR419W
Mitochondrial zinc maintenance protein 1, mitochondrial
Abolishes processing of CYB2 and COX2 and presence of SOM1 in the IMP complex.
In pet ts2858; temperature-sensitive; abolishes processing of CYB2 and presence of SOM1 in the IMP complex.
Mitochondrial inner membrane protease subunit 1
Putative aldehyde dehydrogenase-like protein YHR039C
Chitobiosyldiphosphodolichol beta-mannosyltransferase
SWI/SNF chromatin-remodeling complex subunit SWI1
Dehydrodolichyl diphosphate synthase complex subunit NUS1
Restriction of telomere capping protein 4
In MYO2-2; causes a vacuole inheritance defect.
In MYO2-338; abolishes interaction with YPT11; when associated with G-1484 and G-1511.
In MYO2-338; abolishes interaction with YPT11; when associated with S-1474 and G-1484.
In MYO2-338; abolishes interaction with YPT11; when associated with S-1474 and G-1511.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; S-1529 and G-1546.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; M-1500; S-1529 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; G-1288; S-1529; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with A-1189; M-1500; S-1529; G-1546 and R-1559.
In MYO2-573; causes a mitochondria inheritance defect; when associated with G-1288; M-1500; S-1529; G-1546 and R-1559.
Cysteine sulfenic acid (-SOH) intermediate
No TPx activity, no effect on DOT activity.
Constitutive nuclear entry; when associated with A-522 and A-646.
Constitutive nuclear entry; when associated with A-522 and A-664.
Constitutive nuclear entry; when associated with A-646 and A-664.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-331 and A-540.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-331 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-327; A-540 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-324; A-331; A-540 and A-541.
In scc4m35; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-327; A-331; A-540 and A-541. In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with A-298; D-299; A-313; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; A-313; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; A-324 and D-327.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-313; A-324 and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; A-298; D-299; A-324; D-327; and D-331.
In scc4m7; eliminates centromeric localization of SCC2 in mitotic cells and reduces association of the cohesin subunit SCC1 with the centromere and pericentromere; when associated with K-256; D-299; A-313; A-324; D-327; and D-331.
Uncharacterized RING finger protein YBR062C
tRNA threonylcarbamoyladenosine dehydratase 1
Eukaryotic translation initiation factor 3 subunit B
Impairs interaction with HCR1 and TIF32, impairs interaction of the eIF-3 complex with eIF-1, eIF-2 and the 40S ribosome, and impairs initiation of translation.
Single-stranded DNA-binding protein RIM1, mitochondrial
Mitochondrial intermembrane space cysteine motif-containing protein MIX17
Putative mitochondrial carrier protein PET8
Topoisomerase I damage affected protein 4
ATPase-stabilizing factor 15 kDa protein
Ethionine resistance-conferring protein 1
Required for respiratory growth protein 1, mitochondrial
In COQ4-1; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
In COQ4-2; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
In COQ4-3; abolishes coenzyme Q biosynthesis, but does not affect stability of other COQ polypeptides.
Ubiquinone biosynthesis protein COQ4, mitochondrial
37S ribosomal protein MRP21, mitochondrial
In MRP21-1; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs.
In MRP21-3; suppresses translation defects of mutations in the 5'-untranslated leaders of mitochondrial mRNAs.
Fails to complement the growth and splicing defective, temperature-sensitive phenotype of the null allele at 30 degrees Celsius. No association with U1 snRNP.
No splicing defects. Associates with U1 snRNP; when associated with L-148 and L-152.
No splicing defects. Associates with U1 snRNP; when associated with L-148 and T-150.
No splicing defects. Associates with U1 snRNP; when associated with T-150 and L-152.
Severely temperature-sensitive. Defective in pre-mRNA splicing.
U1 small nuclear ribonucleoprotein 70 kDa homolog
Reduces ATPase activity and interaction with eEF1A. Required for growth at 37 degrees Celsius and causes a 50% reduction of total protein synthesis at permissive temperatures.
Putative uncharacterized protein YOR186W
Dual-specificity protein phosphatase SDP1
ATPase expression protein 1, mitochondrial
Abolishes checkpoint function and interaction with MAD2, MAD3 and CDC20. Benomyl-sensitive phenotype.
Abolishes checkpoint function. Benomyl-sensitive phenotype.
Abolishes checkpoint function. Lowers interaction with BUB1 and MAD3. Benomyl-sensitive phenotype.
Abolishes checkpoint function. No effect on interaction with BUB1 and MAD3. Benomyl-sensitive phenotype.
Abolishes checkpoint function. No effect on interaction with BUB1. Lowers interaction with MAD3. Benomyl-sensitive phenotype.
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Leads to a slower growth at higher temperatures but allows RPL4 assembly into the mature 60S; when associated with E-95.
Y' element ATP-dependent helicase protein 1 copy 3
Y' element ATP-dependent helicase protein 1 copy 7
ATP synthase subunit epsilon, mitochondrial
Abolishes catalytic activity. Reduces the LCA palmitic acid transport activity by 96%. Abolishes the LCB dihydrosphingosine (DHS) transport activity.
Likely essential for ATP utilization. Reduces catalytic activity by 89%. Reduces the LCA palmitic acid transport activity by 71%. has no effect on the LCB dihydrosphingosine (DHS) transport activity.
Reduces enzyme activity by 35%. Partially impairs dimer formation.
Severely impairs t(6)A37 formation. No effect on dimer formation.
Severely impairs t(6)A37 formation. Partially impairs dimer formation.
Severely impairs t(6)A37 formation. Prevents dimerization.
tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial
Required for respiratory growth protein 9, mitochondrial
Alpha N-terminal protein methyltransferase 1
Endoplasmic reticulum junction formation protein lunapark
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-226 and A-244.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-226 and A-247.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-223, A-244 and A-247.
In lnp1-1; causes aberrant ER morphology; when associaetd with A-226, A-244 and A-247.
Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-541.
Depletion of cellular ubiquitin pools and reduced activity of the ubiquitin fusion degradation pathway. Prevents the interaction with CDC48 and UFD1 and thus the degradation of mitochondrial proteins FZO1, MDM34 and MSP1; when associated with A-669.
In ufd3-2; severe reduction in the degradation of short-lived ubiquitin-fusion proteins. No defect in the interaction with CDC48.
Loss of interaction with CDC48 without affecting binding to ubiquitin.
Loss of interaction with HSE1 and ubiquitin without affecting general ubquitination levels. Prevents protease CSP1 sorting into the vacuole.
Loss of interaction with HSE1 without affecting binding to ubiquitin or general ubquitination levels. Prevents protease CSP1 sorting into the vacuole.
No binding to ubiquitin and no interaction with CDC48.
No binding to ubiquitin but does not affect the interaction with CDC48.
No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-222.
No binding to ubiquitin. Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with S-15 and A-265.
No binding to ubiquitin. Reduces cell growth at high temperatures (37 degrees Celsius). Inhibits cell growth at high temperatures and prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 without affecting the interaction with CDC48 and UFD1; when associated with A-222 and A-265.
Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-417.
Prevents binding to mono-ubiquitin, reduces levels of free ubiquitin, prevents the degradation of mitochondrial proteins FZO1, MDM34 and MSP1 and is partially sensitive to misfolded protein or translation inhibition-induced stresses; when associated with D-434.
Protein arginine methyltransferase NDUFAF7 homolog, mitochondrial
Impairs interaction with RAD3, reducing the interaction of TFIIH with the head module and consequently resulting in a reduction of RNA polymerase II CTD 'Ser-5' phosphorylation.
Impairs interaction with SRB4/MED17, SRB6/MED22 and RAD3.
Mediator of RNA polymerase II transcription subunit 11
Results in a decrease of TFIIK and RNA polymerase II occupancies at active promoters; when associated with K-17.
Results in a decrease of TFIIK and RNA polymerase II occupancies at active promoters; when associated with K-24.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs).
Defective in ESCRT-I cargo sorting; reduces MVB12 localization to MVBs; abolishes interaction with MVB12; reduces interaction with SRN2.
No interaction of the ESCRT-I complex with ubiquitin.
Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
26S proteasome regulatory subunit 4 homolog
U3 small nucleolar RNA-associated protein 5
In HRT1-C81Y; defects in ubiquitin ligase activity.
In RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-72.
In RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-81.
Meiosis induction protein kinase IME2/SME1
Histone acetyltransferase type B catalytic subunit
Nuclear polyadenylated RNA-binding protein NAB2
Phosphatidylinositol transfer protein CSR1
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Mitochondrial outer membrane protein OM14
Mediator of replication checkpoint protein 1
Uncharacterized mitochondrial outer membrane protein YDR381C-A
Maintenance of telomere capping protein 5
Eukaryotic peptide chain release factor subunit 1
Abolishes sumoylation in vitro; when associated with R-426.
Abolishes sumoylation in vitro; when associated with R-437.
Prospore formation at selected spindle poles protein 1
tRNA N6-adenosine threonylcarbamoyltransferase
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with D-60 and V-142.
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with D-60 and V-68.
In DCP2-7; impairs mRNA decay at 37 degrees Celsius; when associated with V-68 and V-142.
In strain: V1-09, YJM269, YJM270, YJM326, YJM339, YJM627 and YJM1129.
In strain: YJM 69, YJM270, YJM326 and YJM1129.
In strain: YJM269, YJM270, YJM280, YJM320, YJM326 and YJM1129.
In strain: YJM269, YJM270, YJM326 and YJM1129.
Uncharacterized protein YLR283W, mitochondrial
In FAR1-22P; induces cell cycle arrest in the absence of alpha-factor.
Prevents cell cycle-dependent degradation of FAR1.
Shifts the coenzyme preference of the enzyme from NAD(+) to NADP(+).
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-162.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-159 and A-163.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-155; A-162 and A-163.
No recognition of DNA-binding site and no suppression of calmodulin mutants; when associated with A-159; A-162 and A-163.
Abolished zinc-binding and folding, leading to impaired protein import into peroxisomes.
tRNA-specific adenosine deaminase subunit TAD3
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5
DNA damage-responsive transcriptional repressor RPH1
Cytochrome b-c1 complex subunit 1, mitochondrial
Impairs histone H3 phosphorylation and reduces transcription of some GCN5 regulated genes.
Increases sensitivity to genotoxic agents inducing DNA breaks during replication.
Cytochrome b-c1 complex subunit Rieske, mitochondrial
37S ribosomal protein S12, mitochondrial
Serine/threonine-protein phosphatase 1 regulatory subunit PIG1
Accumulation of aberrant 22S and 23S rRNA intermediates.
Abolishes quinone reductase activity. Little effect on complex assembly.
Impairs respiratory growth and reduces quinone reductase activity.
Reduces SDH FAD content. Probably impairs complex assembly.
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Abolishes exonuclease activity in vitro.
Reduces strongly exonuclease activity; when associated with D-1024; F-1043 and P-1197.
Reduces strongly exonuclease activity; when associated with P-592.
Reduces strongly exonuclease activity; when associated with R-798; D-1024 and F-1043.
Reduces strongly exonuclease activity; when associated with R-798; D-1024 and P-1197.
Reduces strongly exonuclease activity; when associated with R-798; F-1043 and P-1197.
Reduces strongly exonuclease activity; when associated with Y-710.
Ubiquitin carboxyl-terminal hydrolase 15
Vacuolar ATPase assembly integral membrane protein VPH2
Impairs kinase activity; when associated with A-2224.
Impairs kinase activity; when associated with K-2229.
In MEC1-100; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-1179.
In MEC1-100; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-1700.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with P-552 and S-781.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-225 and P-552.
In MEC1-101; impairs both the G1/S and intra-S damage checkpoints but not the G2/M damage checkpoint; when associated with S-225 and S-781.
In MEC1-85; disrupts interaction with RFA1 and severely impairs kinase activity.
In MEC1-87; decreases the level of MEC1 and impairs viability.
Mitochondrial import inner membrane translocase subunit TIM22
Oxidant-induced cell-cycle arrest protein 5
Uncharacterized membrane protein YIL089W
Uncharacterized transcriptional regulatory protein YLL054C
Decreases nucleotide binding by a factor of 2.5 and reduces catalytic activity. Decreases nucleotide binding by a factor of 20 and abolishes catalytic activity; when associated with A-266.
Decreases nucleotide binding by a factor of 4 and reduces catalytic activity. Decreases nucleotide binding by a factor of 20 and abolishes catalytic activity; when associated with A-264.
Reduces ATP turnover and polyP synthesis.
Vacuolar transporter chaperone complex subunit 4
In TRA1-2; when associated with L-604; R-2733; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; P-3145 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; P-3145 and S-3222. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; L-604; R-2733; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
In TRA1-2; when associated with S-241; R-2733; P-3145; S-3222 and G-3302. Defects in its ability to interact with acidic activators.
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
Does not strongly affect the phosphatase inhibitor function but abolishes PPCDC activity.
Phosphopantothenoylcysteine decarboxylase subunit VHS3
Almost complete loss of secreted alpha-agglutinin activity by 10-fold.
Almost complete loss of secreted alpha-agglutinin activity.
Complete loss of alpha-agglutinin activity.
Decreases secreted alpha-agglutinin activity by 10-fold.
Decreases secreted alpha-agglutinin activity by 100-fold.
Decreases secreted alpha-agglutinin activity by 15-fold.
Decreases secreted alpha-agglutinin activity by 2-fold.
Decreases secreted alpha-agglutinin activity by 4-fold.
Decreases secreted alpha-agglutinin activity by 60-fold.
Decreases secreted alpha-agglutinin activity by less than 2-fold.
Decreases secreted alpha-agglutinin activity by more than 20-fold.
Little effect on secreted alpha-agglutinin activity.
Little or no effect on secreted alpha-agglutinin activity.
No effect on secreted alpha-agglutinin activity.
Abolishes acetylation and association with cis-Golgi.
37S ribosomal protein MRP10, mitochondrial
Translation initiation factor eIF-2B subunit delta
Uncharacterized SVF1-like protein YLR225C
37S ribosomal protein S10, mitochondrial
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Hexaprenyl pyrophosphate synthase, mitochondrial
Uncharacterized vacuolar membrane protein YML018C
O-(3'-phospho-DNA)-tyrosine intermediate
Leucine-rich repeat-containing protein SOG2
54S ribosomal protein YmL6, mitochondrial
Thiamine metabolism regulatory protein THI3
In DAM1-1; produces abnormal spindles resulting in growth arrest at 34 degrees Celsius.
Alters kinase activation and biological activity, including enhanced allosteric subunit associations and increased oxidative stress resistance and life span; when associated with A-357.
Alters kinase activation and biological activity, including enhanced allosteric subunit associations and increased oxidative stress resistance and life span; when associated with I-367.
Carbon catabolite-derepressing protein kinase
Exhibits greater activity than wild-type SNFl in an immune complex assay where other associated molecules are present, but exhibits the same activity in a protein blot assay.
Inactivates the kinase activity without affecting protein levels.
Mitochondrial import inner membrane translocase subunit TIM21
In SRB6-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation.
Mediator of RNA polymerase II transcription subunit 22
Mitochondrial import receptor subunit TOM6
Mitochondrial-processing peptidase subunit alpha
Abolishes the interaction with PRP18, and temperature sensitive growth defect.
Abolishes the interaction with PRP18, and temperature sensitive growth defect. Loss of growth and in vitro splicing activity; when associated with A-122 or A-130 or A-135.
Abolishes the interaction with PRP18. Cryo- and thermosensitivity; when associated with A-122 or A-130 or A-135.
Affects the ability to associate with the spliceosome. Loss of growth and in vitro splicing activity; when associated with 215-AAA-217. Cryo- and thermosensitivity; when associated with A-217.
Affects the ability to associate with the spliceosome. Loss of growth, spliceosome binding, and in vitro splicing activity; when associated with 215-AAA-217. Cryo- and thermosensitivity; when associated with A-217.
Glutathione peroxidase-like peroxiredoxin 1
Abolishes peptidase activity, which also hinders autocatalytic processing of the enzyme to the mature form.
In GAL6DB; disrupts nucleic acid-binding activity, but retains normal peptidase activity.
Results in the abnormal cleavage of 3 C-terminal residues instead of only 1 during autocatalytic processing.
Uncharacterized membrane protein YLR050C
Chromatin structure-remodeling complex protein RSC8
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]
Mitochondrial outer membrane protein OM45
In GLC7-1; decreased glycogen accumulation.
Serine/threonine-protein phosphatase PP1-2
Changes protein distribution from the plasma membrane to the cytoplasm and nucleus and alters the cellular life span.
Ran-specific GTPase-activating protein 1
60S ribosome subunit biogenesis protein NIP7
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
Loss of transport activity; when associated with D-112 and D-215. Restored transport activity; when associated with K-109; D-112; K-212; D-215 and K-306.
Loss of transport activity; when associated with D-112 and D-309. Restored transport activity; when associated with K-109; D-112; K-212; K-306 and D-309.
Loss of transport activity; when associated with D-215 and D-309. Restored transport activity; when associated with K-109; K-212; D-215; K-306 and D-309.
Loss of transport activity; when associated with K-109 and K-212. Restored transport activity; when associated with D-109; D-112; K-212; D-215 and D-309.
Loss of transport activity; when associated with K-109 and K-306. Restored transport activity; when associated with D-109; D-112; D-215; K-306 and D-309.
Loss of transport activity; when associated with K-212 and K-306. Restored transport activity; when associated with D-112; K-212; D-215; K-306 and D-309.
Probable pyridoxal 5'-phosphate synthase subunit SNZ3
Schiff-base intermediate with D-ribose 5-phosphate
Putative 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase YLR345W
DNA-directed RNA polymerases I, II, and III subunit RPABC5
ADP-ribosylation factor GTPase-activating protein effector protein 2
Cytochrome B pre-mRNA-processing protein 6
Uncharacterized membrane protein YPL257W
JmjC domain-containing histone demethylation protein 1
RecQ-mediated genome instability protein 1
54S ribosomal protein L11, mitochondrial
Translation initiation factor eIF-2B subunit gamma
Abolishes FBP1 ubiquitination and degradation.
26S proteasome regulatory subunit 7 homolog
Signal recognition particle receptor subunit beta
Loss of function, but still forms a heterodimer with SSZ1 and associates with ribosomes.
Altered inheritance of mitochondria protein 23, mitochondrial
4-hydroxy-4-methyl-2-oxoglutarate aldolase
Regulator of rDNA transcription protein 15
Histone acetyltransferase type B subunit 2
tRNA (guanosine(18)-2'-O)-methyltransferase
In strain: CLIB 219, CLIB 410, CLIB 413 and YIIc17 haplotype Ha2.
In strain: YIIc12 and YIIc17 haplotype Ha1.
Multicopy suppressor of SEC21 protein 28
Serine/arginine (SR)-type shuttling mRNA binding protein HRB1
Probable alpha-1,6-mannosyltransferase MNN11
Putative oligopeptide transporter YGL114W
Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase
Loss of MCM2-7 complex helicase activity.
Reduces MCM2-7 complex helicase activity. Abolishes MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM3 A-415.
Ferric/cupric reductase transmembrane component 2
Impairs cytosolic tRNA-cleaving activity; when associated with F-160.
Impairs cytosolic tRNA-cleaving activity; when associated with F-87.
Increased recombination centers protein 22
Trafficking protein particle complex subunit 33
Sporulation-specific with a leucine zipper motif protein 1
Ubiquitin-conjugating enzyme suppressor 1
In gwt1-10; impairs the transport of detergent-resistant microdomain-associated membrane proteins TAT2 and FUR4.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-362 and A-479.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and A-479.
In gwt1-16; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with S-330 and P-362.
In gwt1-20; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins.
In gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with D-259.
In gwt1-28; temperature sensitive mutant that induces a delay in export of GPI-anchored proteins; when associated with P-209.
Resistant to the drug 1-[4-butylbenzyl]isoquinoline (BIQ).
Interferes with correct plasma membrane localization.
Loss of function; when associated with Q-323.
Loss of function; when associated with Q-324.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
RNA polymerase II transcription factor B subunit 3
Probable dolichyl-phosphate-mannose--protein mannosyltransferase 7
Disrupts the interaction with YTM1 and cannot sustain growth.
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-470.
Weakens the interaction with YTM1, impairs pre-rRNA processing and leads to a deficiency in 60S ribosomal subunits. Disrupts the interaction with YTM1 and cannot sustain growth; when associated with R-790.
AbA resistant; when associated with F-137.
AbA resistant; when associated with Y-157.
Inositol phosphorylceramide synthase catalytic subunit AUR1
Abolishes ubiquitination and subsequent degradation.
Affects phosphorylation and the interaction with ESP1.
Ran-specific GTPase-activating protein 30
Loss of activity. No effect on interaction with LCB2.
No effect on stability. Destabilizes LCB2.
No effect on stability. No effect on LCB2 stabilization.
No effect on stability. Partially stabilizes LCB2.
DNA polymerase epsilon catalytic subunit A
In POL2-18; temperature-sensitive mutant.
In POL2-9; temperature-sensitive mutant.
Increases rates of C-to-A transversion substitutions.
No pyridoxal 5'-phosphate synthesis activity. Retains ability to isomerize dihydroxyacetone phosphate to glyceraldehyde 3-phosphate.
Pyridoxal 5'-phosphate synthase subunit SNZ1
Ferric reductase transmembrane component 4
The measured mass is that of the monomethylated protein.
Deficiency in ubiquitin-protein conjugate formation.
Deficiency in ubiquitin-protein conjugate formation. Loss of DNA repair function.
Calcium/calmodulin-dependent protein kinase I
Abolishes enzyme activity; when associated with A-11 and A-505.
Abolishes enzyme activity; when associated with A-11 and A-511.
Abolishes enzyme activity; when associated with A-505 and A-511.
Causes accumulation in vacuoles and abolishes sorting into internal vesicles in late endosomes.
Arf guanine nucleotide exchange factor SYT1
Eukaryotic translation initiation factor 3 subunit A
Reduces interaction of eIF-3 with the 40S ribosome.
Thiosulfate sulfurtransferase RDL2, mitochondrial
Benzil reductase ((S)-benzoin forming) IRC24
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-177.
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-397.
Leads to a prolonged S phase and a shortened passage through G1; when associated with A-177 and A-397.
Predominantly cytoplasmic; when associated with A-159, A-177 and S-397.
Significant reduction in phosphorylation; when associated with A-397. Complete inhibition of phosphorylation; when associated with A-159 and A-397. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-397. Predominantly cytoplasmic; when associated with S-99, A-159 and A-397.
Significant reduction in phosphorylation; when associated with A-397. Complete inhibition of phosphorylation; when associated with A-177 and A-397. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-177 and A-397. Predominantly cytoplasmic; when associated with S-99, A-177 and A-397.
Significant reduction in phosphorylation; when associated with either A-159 or A-177. Complete inhibition of phosphorylation; when associated with A-159 and A-177. Leads to a prolonged S phase and a shortened passage through G1; when associated with A-159 and A-177. Predominantly cytoplasmic; when associated with S-99; A-159 and A-177.
Peptidyl-prolyl cis-trans isomerase CPR6
Abolishes the ability to complement a yeast strain carrying a deletion for this gene.
Succinate dehydrogenase assembly factor 1, mitochondrial
Inositol phosphorylceramide synthase regulatory subunit KEI1
Abolishes binding to SUI3; probably impairs the conversion of eIF-2-GDP to eIF-2-GTP.
Translation initiation factor eIF-2B subunit epsilon
Putative glutamine amidotransferase YLR126C
ADP-ribosylation factor GTPase-activating protein GCS1
In GCS1-1; severely affect the function.
In cly5; temperature-sensitive mutation that cause cell lysis at high temperature.
In cly7; temperature-sensitive mutation that cause cell lysis at high temperature.
Endoplasmic reticulum transmembrane protein 3
In strain: SK1, V1-09, YJM230, YJM320 and YJM339.
In strain: YJM1129, YJM269, YJM270, YJM326 and YJM627.
Vacuolar protein sorting-associated protein 9
Mitochondrial phosphate carrier protein, N-terminally processed
N-acetylserine; in Mitochondrial phosphate carrier protein, N-terminally processed
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial
Histidine biosynthesis trifunctional protein
In allele AFT1-1UP; which is constitutively activated.
Iron-regulated transcriptional activator AFT1
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82
Chromatin modification-related protein YNG2
Serine/threonine-protein phosphatase 4 regulatory subunit 2
Cytochrome c oxidase assembly factor 3, mitochondrial
Uncharacterized mitochondrial hydrolase FMP41
tRNA-splicing endonuclease subunit SEN54
Structural maintenance of chromosomes protein 3
Maintenance of telomere capping protein 7
Dual specificity protein phosphatase PPS1
DNA repair/transcription protein MET18/MMS19
Mitochondrial distribution and morphology protein 32
Topoisomerase I damage affected protein 2
Abolished protein import in peroxisomes.
Does not affect assembly of the PEX2-PEX10-PEX12 complex.
Does not affect assembly of the PEX2-PEX10-PEX12 complex; causes peroxisomal import defects when associated with mutations in PEX10.
In DCP1-17; partial loss of mRNA-decapping activity.
In DCP1-19; partial loss of mRNA decapping activity. In DCP1-43; strong loss of mRNA-decapping activity; when associated with A-48 and A-50.
In DCP1-1; strong loss of mRNA decapping activity.
In DCP1-25; partial loss of mRNA-decapping activity. In DCP1-44; strong loss of mRNA-decapping activity; when associated with A-48 and A-50.
In DCP1-2; strong loss of mRNA decapping activity at 36 degrees Celsius.
In DCP1-31; partial loss of mRNA decapping activity.
In DCP1-32; partial loss of mRNA decapping activity.
In DCP1-33; partial loss of mRNA decapping activity.
In DCP1-33; strong loss of mRNA decapping activity.
In DCP1-35; partial loss of mRNA decapping activity.
In DCP1-4; partial loss of mRNA decapping activity. In DCP1-43; strong loss of mRNA-decapping activity; when associated with A-187 and A-188. In DCP1-44; strong loss of mRNA-decapping activity; when associated with A-216 and A-219.
Partial loss of mRNA-decapping activity.
Strong loss of mRNA-decapping activity; when associated with A-217 and A-221.
Strong loss of mRNA-decapping activity; when associated with A-37; A-38 and A-217.
Strong loss of mRNA-decapping activity; when associated with A-37; A-38 and A-221.
High-affinity nicotinic acid transporter
Increased localization to the cytoplasm.
Slightly increased localization to the cytoplasm.
Slightly increased localization to the nucleus.
Maintenance of telomere capping protein 1
Putative uncharacterized protein YAL064W
SWI/SNF global transcription activator complex subunit SWP82
Serine/threonine-protein phosphatase 2B catalytic subunit A2
Impairs interaction with ATG5and autophagy.
Significantly reduces autophagic activity; when associated with A-101.
Significantly reduces autophagic activity; when associated with A-102.
Significantly reduces autophagic activity; when associated with A-104 and A-108.
Significantly reduces autophagic activity; when associated with A-104 and A-112.
Significantly reduces autophagic activity; when associated with A-108 and A-112.
Does not affect cell wall incorporation.
Results in complete loss of cell wall incorporation.
Results in the incorporation of KEX2-unprocessed precursor protein into the cell wall.
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit
Abolishes interaction with RSA4. Blocks production of mature 60S subunits, and causes the accumulation of pre-60S particles.
5-glutamyl coenzyme A thioester intermediate
Actin cytoskeleton-regulatory complex protein END3
In strain: SK1, YJM 269, YJM 270, YJM 280, YJM 320, YJM 326, YJM 339, YJM 421, YJM 627, YJM 789 and YJM 1129.
In strain: YJM 280, YJM 320, YJM 326, YJM 627, YJM 789 and YJM 1129.
ADA histone acetyltransferase complex component 2
Transmembrane E3 ubiquitin-protein ligase 1
Serine/threonine-protein kinase HAL4/SAT4
Cytochrome c oxidase subunit 13, mitochondrial
Endoplasmic reticulum membrane protein 65
91% decrease of DGPP phosphatase activity.
Complete loss of DGPP phosphatase activity.
Diacylglycerol pyrophosphate phosphatase 1
Branched-chain-amino-acid aminotransferase, mitochondrial
Bifunctional purine biosynthesis protein ADE17
Iron-regulated transcriptional activator AFT2
Mitochondrial peculiar membrane protein 1
Meiotic sister-chromatid recombination protein 6, mitochondrial
Putative uncharacterized protein YOL014W
Probable metabolite transport protein YFL040W
Trafficking protein particle complex II-specific subunit 120
Meiotic sister chromatid recombination protein 1
Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-98.
Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-9 and A-99.
Abolishes CAT tail synthesis and heat shock response, but still binds to 60S ribosomal subunits and supports LTN1-dependent ubiquitination of nascent chains; when associated with A-98 and A-99.
No effect on protein abundance. Decreased CAT tail synthesis.
No effect on protein abundance. Decreased CAT tail synthesis. No effect on ribosome-associated ubiquitin-dependent protein catabolic process.
No effect on protein abundance. Loss of CAT tail synthesis. Loss of ribosome-associated ubiquitin-dependent protein catabolic process.
Ribosome quality control complex subunit 2
Mitochondrial holo-[acyl-carrier-protein] synthase
Uncharacterized mitochondrial carrier YFR045W
Decreases ATG8-PE comjugation and autophagy.
Instead of the formation of an intermediate complex with a thiol ester bond between ATG3 (E2-like enzyme) and ATG8 (substrate), a stable complex with an O-ester bond is formed.
Loss of interaction with ATG8 and defect in autophagy and Cvt pathway.
In NH1; causes resistance to the antifungal antibiotic pradimicin.
Reduces binding of the 4-aspartylphosphate of SLN1.
In RRP4-1; temperature-sensitive(ts) lethal mutation.
Transcription activator of gluconeogenesis ERT1
Probable alpha-1,6-mannosyltransferase MNN10
Inhibits splicing; when associated with S-284; N-362 and S-738 in X10SSS VDE.
Inhibits splicing; when associated with S-284; S-737 and S-738 in X10SSS VDE.
Reduces splicing reaction speed. Inhibits splicing; when associated with N-362; S-737 and S-738 in X10SSS VDE.
Reduces splicing reaction speed. Inhibits splicing; when associated with S-284; N-362 and S-737 in X10SSS VDE.
V-type proton ATPase catalytic subunit A, 1st part
V-type proton ATPase catalytic subunit A, 2nd part
UDP-N-acetylglucosamine transferase subunit ALG13
Increased recombination centers protein 21
Abolishes poly(A) RNA degradation; when associated with A-188.
Abolishes poly(A) RNA degradation; when associated with A-190.
No cell-cycle stop in response to glucose deprivation.
In rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with L-132.
In rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with P-377.
Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
Vacuolar protein sorting-associated protein 72
37S ribosomal protein S26, mitochondrial
Peroxisomal adenine nucleotide transporter 1
Eukaryotic translation initiation factor 3 subunit J
Increased association of the eIF-3 complex and 40S ribosomes.
Putative uncharacterized protein YBR285W
Uncharacterized glycosyl hydrolase YBR056W
Impairs correct nuclear localization; when associated with T-19 and T-20.
Impairs correct nuclear localization; when associated with T-19 and T-23.
Impairs correct nuclear localization; when associated with T-20 and T-23.
Leads to temperature-dependent mislocalization of nucleoporins (nups) and the pore-membrane protein POM152.
Lethal and prevents release of U14 snoRNA from pre-ribosomes.
Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-225.
Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-227.
37S ribosomal protein MRP13, mitochondrial
Abolishes the ability to suppress glucose-induced cAMP signaling when overexpressed.
Decreases the ability to suppress glucose-induced cAMP signaling when overexpressed.
Inorganic pyrophosphatase, mitochondrial
Carnitine O-acetyltransferase, mitochondrial
Peroxisomal acyl-coenzyme A thioester hydrolase 1
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
In ALG3-1; leads to an underglycosylation of secretory proteins.
Cysteine-rich and transmembrane domain-containing protein YDR034W-B
Phosphatidylglycerol/phosphatidylinositol transfer protein
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Loss of function; when associated with A-1236.
Loss of function; when associated with A-608.
Mediator of RNA polymerase II transcription subunit 13
Probable transcription repressor protein RGM1
Translation initiation factor eIF-2B subunit beta
Glycine dehydrogenase (decarboxylating), mitochondrial
RNA polymerase I-specific transcription initiation factor RRN7
Uncharacterized ATP-dependent helicase IRC5
Putative aryl-alcohol dehydrogenase AAD3
Probable proteasome subunit alpha type-7
Chromatin modification-related protein EAF3
Transcription initiation factor TFIID subunit 7
Decreases the interaction with CDC123, when associated with A-220.
Decreases the interaction with CDC123, when associated with A-350.
Decreases the interaction with CDC123, when associated with L-223.
Decreases the interaction with CDC123, when associated with S-345.
RNA polymerase II-associated protein RBA50
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Cytochrome B pre-mRNA-processing protein 2
In SRB7-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation.
Mediator of RNA polymerase II transcription subunit 21
DNA-directed RNA polymerase I subunit RPA14
Disrupts interaction with STE50 and abolishes signal transduction.
ATP synthase assembly factor FMC1, mitochondrial
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Actin-related protein 2/3 complex subunit 5
Succinate dehydrogenase assembly factor 4, mitochondrial
Abolishes phosphorylation; when associated with A-12; A-16; A-157; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-227.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-12; A-42; A-157; A-169; A-173; A-176; A-227 and A-239.
Abolishes phosphorylation; when associated with A-16; A-42; A-157; A-169; A-173; A-176; A-227 and A-239.
Decreases propensity to stall ribosomes.
Putative mitochondrial carnitine O-acetyltransferase
Increased recombination centers protein 15
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
Mitochondrial distribution and morphology protein 34
Guanine nucleotide-binding protein subunit gamma
Acetolactate synthase small subunit, mitochondrial
DNA-directed RNA polymerase I subunit RPA34
Impairs RNA-binding (at the proposed ring entry site).
Impairs RNA-binding (at the proposed ring exit site).
54S ribosomal protein L16, mitochondrial
Cx9C motif-containing protein 4, mitochondrial
Probable transcriptional regulatory protein STB4
Decreases the amount of 40S ribosomal subunit; when associated with R-144; A-244 and A-246.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; A-244 and A-246.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; R-144 and A-244.
Decreases the amount of 40S ribosomal subunit; when associated with T-85; R-144 and A-246.
Greatly diminishes transcriptional activation.
Serine hydroxymethyltransferase, cytosolic
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Reduced growth at 25 degrees and lethal at 37 degrees.
Chromatin modification-related protein EAF5
RNA polymerase II subunit B1 CTD phosphatase RTR2
Dominant negative mutant; displays respiratory defects that are higher than the BOL1 BOL3 double mutant.
In strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6044, DBVPG6763, DBVPG6765, SK1, Y55 and YPS128.
In strain: DBVPG1135, DBVPG1373, DBVPG1378, DBVPG1788, DBVPG1794, DBVPG1853, DBVPG3051, DBVPG6763, DBVPG6765 and SK1.
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with S-315 and S-393.
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with Y-105 and S-315.
Decreases the amount of 40S ribosomal subunits at 37 degrees Celsius; when associated with Y-105 and S-393.
Increases 3-fold the ATPase activity and a higher RNA/DNA heteroduplexes unwinding activity in vitro. Leads to 13% of wild-type ATPase activity and lower RNA/DNA heteroduplexes unwinding activity in vitro; when associated with A-92.
Lethal in vivo and impairs ATPase with 2-5% of wild-type ATPase activity, a 20-fold higher KM for ATP and prevents RNA/DNA heteroduplexes unwinding activity in vitro. Leads to 13% of wild-type ATPase activity and higher RNA/DNA heteroduplexes unwinding activity in vitro; when associated with A-389.
Lethal in vivo and leads to 70-80% of wild-type ATPase activity, a 2-fold lower KM for ATP and a reduced RNA/DNA heteroduplexes unwinding activity in vitro.
Lethal in vivo and leads to less than 30% of wild-type ATPase activity and a 2-fold lower KM for ATP in vitro.
Slow growth at 18 and 16 degrees Celsius, no growth at 14 degrees Celsius and drastic decrease of the amount of 40S ribosomal subunits at 30 degrees Celsius in vivo. Leads to 70-80% of wild-type ATPase activity, a 2-fold lower KM for ATP and a slightly reduced RNA/DNA heteroduplexes unwinding activity in vitro.
Abolishes ATPase activity and proton transport, but does not affect complex assembly.
Reduces assembly of V-ATPase complexes and reduces ATPase activity of the assembled complexes.
V-type proton ATPase subunit a, vacuolar isoform
Mediates translocation across the endoplasmic reticulum, renders the enzyme inactive during transit, and targets the molecule to the vacuole
N-linked (GlcNAc...) (high mannose) asparagine
Nascent polypeptide-associated complex subunit alpha
Mitochondrial 2-methylisocitrate lyase ICL2
Decreases catalytic activity 5-fold, but retains substrate binding properties.
In ram2-1; completely abolishes farnesyltransferase activity, but still has about 30% geranyl-geranyl transferase activity.
Increases KM for peptide substrate 4-fold, but retains catalytic activity.
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
Phosphatidylinositol transfer protein PDR16
Disrupts the heterotetrameric state and becomes hypersensitive to MMS; when associated with A-55 or 78-AA-79.
Disrupts the heterotetrameric state and becomes hypersensitive to MMS; when associated with A-62.
Cytochrome c oxidase assembly protein COX16, mitochondrial
Uncharacterized mitochondrial protein RF1
54S ribosomal protein L25, mitochondrial
Uncharacterized membrane protein YLR326W
Probable serine/threonine-protein kinase IKS1
No interaction with CTK2. Still interacts with CTK1.
D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2
Postreplication repair E3 ubiquitin-protein ligase RAD18
Abolished E3 ubiquitin-protein ligase activity, leading to impaired peroxisome biogenesis.
Abolished E3 ubiquitin-protein ligase activity.
Abolished interaction with PEX12, leading to prevent E3 ubiquitin-protein ligase activity.
Reduced E3 ubiquitin-protein ligase activity.
Alters cofactor preference of the enzyme to be able to use as well NAD instead of NADP.
NADPH-dependent methylglyoxal reductase GRE2
Mitochondrial import receptor subunit TOM7
Glutathione peroxidase-like peroxiredoxin HYR1
Interchain (with C-598 in YAP1); transient
Vacuolar protein sorting-associated protein 33
Decreases gene expression from the ho locus.
In mkt1-19; HO gene expression is decreased.
Reduced viability upon starvation protein 161
Abrogates the introduction of ceramides into the GPI anchor.
Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium.
Impairs the introduction of ceramides into the GPI anchor.
No effect on introduction of ceramides into the GPI anchor.
Cytochrome c mitochondrial import factor CYC2
tRNA (cytidine(32)-2'-O)-methyltransferase non-catalytic subunit TRM732
ATP-dependent 6-phosphofructokinase subunit beta
Abolishes sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'D-724' in subunit alpha.
Drastically reduces sensitivity of the holoenzyme to ATP inhibition.
Increases the KM for fructose 6-phosphate 50 fold.
Reduces maximal activity of the holoenzyme by 30%.
Reduces maximal activity of the holoenzyme by 50%. Completely abolishes catalytic activity; when associated with 'T-309' or 'S-356' in subunit alpha.
Reduces maximal activity of the holoenzyme by less than 25%.
Reduces sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'S-859' in subunit alpha.
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase
C-terminal peroxisome targeting signal (PTS1)
Lethal(2) giant larvae protein homolog SRO77
Probable aryl-alcohol dehydrogenase AAD4
tRNA (uracil(54)-C(5))-methyltransferase
Mannan polymerase complexes subunit MNN9
Reduced activity of the M-Pol I complex.
Loss of function; when associated with S-296.
Loss of function; when associated with S-298.
Loss of function; when associated with S-307.
Loss of function; when associated with S-317.
Loss of function; when associated with S-330.
Loss of function; when associated with S-340.
Mitochondrial intermembrane space import and assembly protein 40
Grand meiotic recombination cluster protein 2
In strain: CLIB 556 haplotype Ha1 and CLIB 630.
Almost complete loss of enzyme activity.
Retains more than 40% of the wild-type enzyme activity.
54S ribosomal protein L13, mitochondrial
Nicotinamide mononucleotide adenylyltransferase
GTP:AMP phosphotransferase, mitochondrial
Probable ATP-dependent RNA helicase DHR1
Non-structural maintenance of chromosome element 4
Centromere DNA-binding protein complex CBF3 subunit A
Altered inheritance of mitochondria protein 11
Cytosolic Fe-S cluster assembly factor NAR1
Impaired maturation of cytosolic Fe/S proteins. Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416.
Impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-416.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-177 or A-231.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-177.
Reduced iron content and strongly impaired maturation of cytosolic Fe/S proteins; when associated with S-412.
Weakly reduced iron content and impaired maturation of cytosolic Fe/S proteins. Strongly impaired maturation of cytosolic Fe/S proteins; when associated with A-62 or A-65.
Putative uncharacterized protein YMR321C
54S ribosomal protein L31, mitochondrial
Eliminates arginine-specific stalling of ribosomes at the termination codon.
Increases YTA7 binding to the HTA1 open reading frame outside of S-phase. Increases localization of RTT106 and RSC8 to the HTA1 gene locus. Increases nucleosome occupancy at core histone loci. Decreases transcription of HTA1.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370 and A-380.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-259; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-241; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-230; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-212; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-67; A-94; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-11; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Severely decreases phosphorylation, causes a G2/M transition delay, and leads to sensitivity to 6-azauracil (impairs transcriptional elongation); when associated with A-67; A-94; A-212; A-230; A-241; A-259; A-285; A-304; A-369; A-370; A-380 and A-445.
Slightly reduces affinity for poly-U RNA ends.
General transcription and DNA repair factor IIH subunit SSL1
Transcription initiation factor IIF subunit alpha
Vacuole transporter chaperone complex subunit 5
Defective in ATP hydrolysis. Missorting of vacuolar proteins.
Inhibits membrane protein sorting to the vacuole.
No ATP hydrolysis. Missorting of vacuolar proteins.
Vacuolar protein sorting-associated protein 4
Peroxisomal long-chain fatty acid import protein 1
2-isopropylmalate synthase 2, mitochondrial
Mitochondrial respiratory chain complexes assembly protein YTA12
Putative magnesium-dependent phosphatase YER134C
Abolihes binding to acetylated histones.
Does not affect binding to the proteasome.
Does not affect viability in the presence of cycloheximide.
Loss of function; abolishes binding to the proteasome.
In SRP1-1; temperature-sensitive mutant.
In SRP1-2; temperature-sensitive mutant.
In SRP1-31; temperature-sensitive mutant; reduced growth rate and chromosome loss.
In SRP1-3; temperature-sensitive mutant.
In SRP1-49; temperature-sensitive mutant; alteration in nucleolar and microtubule morphology.
In SRP1-54; temperature-sensitive mutant; reduced growth rate.
In MLC1-11; abolishes interaction with MYO2 and IQG1 and reduces interaction with MYO1. Leads to mislocalization of IQG1 and a severe defect in cytokinesis.
In MLC1-1; causes a defect in septum formation.
In MLC1-5; causes a defect in septum formation.
In MLC1-93; reduces interaction with MYO1, but does not cause any defect in cytokinesis.
Guanine nucleotide-binding protein subunit beta 1
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831 and A-842.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831, A-842 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-831, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-804, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-802, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-657, A-804, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-19, A-802, A-804, A-831, A-842, A-948 and A-969.
Abolished autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation; when associated with A-657, A-802, A-804, A-831, A-842, A-948 and A-969.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-113 and R-121.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-113 and R-138.
Abolished ubiquitination by the SCF(MET30) complex and subsequent degradation; when associated with R-121 and R-138.
Abolishes interaction with ATG11 and disrupts Cvt, but not bulk autophagy.
Does not affect ubiquitination by the SCF(MET30) complex and subsequent degradation.
Impaired selective autophagy. Does not affect ubiquitination by the SCF(MET30) complex.
In Acidic mutant; abolished autophagy caused by defects in phospholipid scramblase activity.
In Basic mutant; impaired autophagy; when associated with A-560 and A-576.
In Basic mutant; impaired autophagy; when associated with A-560 and A-723.
In Basic mutant; impaired autophagy; when associated with A-576 and A-723.
In Entrance 1 mutant; does not affect autophagy; when associated with A-385.
In Entrance 1 mutant; does not affect autophagy; when associated with S-545.
In Entrance 2 mutant; does not affect autophagy.
In Entrance 3 mutant; impaired autophagy possibly caused by mislocalization; when associated with 497-A--A-501.
In Entrance 3 mutant; impaired autophagy possibly caused by mislocalization; when associated with A-392.
In Hexamer mutant; does not affect autophagy.
In Junction mutant; does not affect autophagy.
In Wall mutant; abolished autophagy caused by defects in phospholipid scramblase activity.
Phospho-mimetic mutant; increased selective autophagy. Prevents ubiquitination by the SCF(MET30) complex.
Non-classical export protein 2 homolog 1
Probable proline--tRNA ligase, mitochondrial
Abolishes the physical interaction with ARG82 and impairs function; when associated with G-94 and D-95.
Arginine metabolism regulation protein I
Decreases the physical interaction with ARG82.
Decreases the physical interaction with ARG82. Abolishes the physical interaction with ARG82 and impairs function; when associated with D-95 and R-103.
Decreases the physical interaction with ARG82. Abolishes the physical interaction with ARG82 and impairs function; when associated with G-94 and R-103.
Ankyrin repeat-containing protein YGL242C
Uncharacterized endoplasmic reticulum membrane protein YNL046W
Impairs basic amino-acids transport and regulation by these amino-acids.
Putative uncharacterized protein YLR264C-A
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-336.
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-336.
Mild disruption of iron-sulfur-binding. Strong disruption of iron-sulfur-binding; when associated with S-474. Strong disruption of iron-sulfur-binding, leading to destabilization of the protein and preventing its purification; when associated with S-417 or S-434.
54S ribosomal protein L50, mitochondrial
Abolished disulfide bond, leading to impaired conformational state; when associated with A-272.
Abolished disulfide bond, leading to impaired conformational state; when associated with A-332.
Significantly decreased protease activity.
Ubiquitin ligase complex F-box protein UFO1
Abolishes PPIase activity and fails to activate PP2A phosphatases.
Serine/threonine-protein phosphatase 2A activator 1
Trafficking protein particle complex subunit 23
Trafficking protein particle complex subunit 20
Altered inheritance of mitochondria protein 34, mitochondrial
Glycolytic genes transcriptional activator GCR1
In APC11-13; G2/M cell cycle arrest at 37 degrees Celsius.
In mutant W7 which is respiratory deficient.
Impaired ability to grow on nonfermentable carbon sources.
Inability to grow on nonfermentable carbon sources.
Ubiquinone biosynthesis protein COQ9, mitochondrial
Mitochondrial biogenesis regulation protein 1
In sgo1-100; induces a lack of response when chromosomes that are not under tension.
In sgo1-700; induces a lack of response when chromosomes that are not under tension.
In USE1-0; slows down protein transport from the endoplasmic reticulum to the Golgi at 37 degrees Celsius.
ATP synthase subunit alpha, mitochondrial
tRNA-dihydrouridine(20) synthase [NAD(P)+]
Does not inhibit interaction with TTI1 or TTI2. Inhibits interaction with TTI1 or TTI2; when associated with E-345.
Does not inhibit weakly interaction with TTI1 or TTI2. Inhibits interaction with TTI1 or TTI2; when associated with Q-333.
ATPase expression protein 2, mitochondrial
Suppresses a T-to-C nucleotide transition mutation in the 5' untranslated region of OLI1 mRNA localized 16 nucleotides upstream of the OLI1 reading frame.
RNA polymerase II subunit A C-terminal domain phosphatase SSU72
Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-36 and R-37.
Reduces sumoylation and impairs interaction with SIZ2, WSS1 and ULS1, when associated with R-61 and R-62.
In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and N-72.
In MSL5-2; temperature-sensitive; growth defect; when associated with G-60 and S-155.
In MSL5-2; temperature-sensitive; growth defect; when associated with N-72 and S-155.
In MSL5-5; cold-sensitive; growth defect; when associated with D-195.
In MSL5-5; cold-sensitive; growth defect; when associated with V-258.
Cruciform cutting endonuclease 1, mitochondrial
Sorting assembly machinery 35 kDa subunit
37S ribosomal protein RSM28, mitochondrial
In RSM28-1; allows translation of defective COX2 mRNAs.
Cytochrome c1, heme protein, mitochondrial
Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-289.
Loss of CYT1 and COB from the bc1 complex; when associated with L-288 and L-296.
Loss of CYT1 and COB from the bc1 complex; when associated with L-289 and L-296.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1
Abolishes the intracellular punctate localization and decreases the cytoplasm to vacuole transport.
In VTI1-11; displays a block in traffic to the PVC and an additional defect in retrograde traffic to the cis-Golgi; when associated with F-155.
In VTI1-11; displays a block in traffic to the PVC and an additional defect in retrograde traffic to the cis-Golgi; when associated with G-145.
In VTI1-12; blocks constitutively the traffic from the late Golgi to the vacuole and blocks the transport to the cis-Golgi compartment at nonpermissive temperature; when associated with R-158.
In VTI1-12; blocks constitutively the traffic from the late Golgi to the vacuole and blocks the transport to the cis-Golgi compartment at nonpermissive temperature; when associated with S-141.
In VTI1-1; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with K-145.
In VTI1-1; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with R-148.
In VTI1-2; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with P-130.
In VTI1-2; exhibits defects in TGN to PVC transport at nonpermissive temperature; when associated with T-151.
In SPP91-1; corrects the PRP9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype.
Impairs interaction with ARL1 and Golgi localization.
ATP-dependent DNA helicase HMI1, mitochondrial
Pentatricopeptide repeat-containing protein PET309, mitochondrial
Methylenetetrahydrofolate dehydrogenase [NAD(+)]
Putative tyrosine-protein phosphatase OCA6
Abolishes both palmitoylation and N-myristoylation.
Abolishes palmitoylation but not N-myristoylation.
Causes a specific mating defect in alpha cells.
Causes constitutive activation of the pheromone response pathway.
Enhances the rate of GDP for GTP exchange and slows hydrolysis of GTP, resulting in a constitutively active form that down-regulates the pheromone response independently of the pheromone receptor.
Forms a nondissociable complex with the pheromone receptor in response to receptor activation, resulting in reduced pheromone responsiveness.
Guanine nucleotide-binding protein alpha-1 subunit
Has increased GTP occupancy and moderately reduces hydrolysis of GTP, resulting in a constitutively active form that down-regulates the pheromone response and causes hyperadaptation to pheromone.
Impairs pheromone signaling in a and alpha cells.
Impairs pheromone signaling specifically in a cells.
In GPA1(SST); weakens interaction to SST2 and blocks its negative regulatory effect.
Leads to a hypersensitive signaling phenotype resulting in greatly enhanced signal at low alpha-factor concentrations.
Prevents GDP to GTP exchange; suppressor of L-323.
Prevents hydrolysis of GTP; eliminates the interaction with STE4 and constitutively activates the pheromone response pathway.
Reduces ligand-dependent pheromone signaling.
Slightly reduces ligand-dependent pheromone signaling.
Suppressor of L-323; does not prevent GTP binding to GPA1.
Guanine nucleotide-binding protein subunit beta 2
Hypoxanthine-guanine phosphoribosyltransferase
Ferric reductase transmembrane component 3
Leads to mRNA turnover defect and no growth at 37 degrees Celsius.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius. Impairs RNA binding in vitro.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-89. Impairs RNA binding in vitro.
Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-91. Impairs RNA binding in vitro.
Maintenance of telomere capping protein 3, mitochondrial
Mediator of RNA polymerase II transcription subunit 7
Dolichyl-phosphate beta-glucosyltransferase
U6 snRNA-associated Sm-like protein LSm4
54S ribosomal protein RML2, mitochondrial
Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit.
Abolishes RSP5 binding site and consequently ubiquitination.
Abolishes formation of ubiquitinated protein forms.
Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin, aggregation, and prion-inducing ability.
Reduces, but does not abolish the ability to promote [PSI+] induction.
Ran-specific GTPase-activating protein 2
Uncharacterized membrane protein YHR175W-A
Probable phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TEP1
Loss of function; when associated with S-56.
Loss of function; when associated with S-59.
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20
Bifunctional purine biosynthesis protein ADE16
Target of rapamycin complex 2 subunit BIT61
Altered inheritance of mitochondria protein 32
Mitochondrial outer membrane protein porin 2
Changed the substrate specificity of the transporter from zinc to iron.
Actin cytoskeleton-regulatory complex protein SLA1
PMA1 stabilization in the Golgi protein 1
Uncharacterized GTP-binding protein YGR210C
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-138, A-141, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-145 and A-282.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-145 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-141, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-138, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-14, A-16, A-141, A-145, A-282 and A-292.
Impairs largely arsenic-induced phosphorylation, shows strong sensitivity to arsenic treatment and a strong, although not complete, reduction in arsenite-induced expression of ARR2 and ARR3; when associated with A-16, A-138, A-141, A-145, A-282 and A-292.
Impairs nuclear relocalization in response to arsenic and transcriptional activity, and leads to arsenic hypersensitivity.
Impairs nuclear relocalization in response to arsenic and transcriptional activity, and leads to arsenic hypersensitivity. Loses most of the arsenic-binding capacity; when assciated with A-274.
Retains about 60% of activity; when assocoated with A-92.
Retains about 60% of activity; when assocoated with A-93.
Cells sensitive to calcofluor, caffeine and Congo red.
Mitochondrial distribution and morphology protein 31
Desensitizes cells to H(2)O(2) treatment.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Impairs ubiquitin conjugation, DNA double-strand breaks formation during meiosis and histone H3-K79 methylation.
Induces apoptotic-like features including chromatin condensation.
In strain: SK1, V1-09, YJM339 and YJM627.
Mitochondrial import receptor subunit TOM70
In DBP5-1; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-220.
In DBP5-1; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with S-466.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with L-236 and F-245.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-171 and F-245.
In DBP5-2; accumulates poly(A)+ RNA in the nucleus at 37 degrees Celsius; when associated with P-171 and L-236.
In RAT8-2; accumulates poly(A)+ RNA in the nucleus at 16 and 37 degrees Celsius.
In RAT8-3; accumulates poly(A)+ RNA in the nucleus at 16 and 37 degrees Celsius.
In RAT8-7; accumulates poly(A)+ RNA in the nucleus at 16 degrees Celsius.
UTP--glucose-1-phosphate uridylyltransferase
ADP-ribosylation factor-binding protein GGA2
Inner membrane assembly complex subunit 17
Abolishes ubiquitination by HEL2/UBC4. Defective activation of the ribosome quality control (RQC) pathway. Sensitive to anisomycin (stalls ribosomes in the rotated state).
COX3 mRNA-specific translational activator PET494
Mitochondrial intermembrane space cysteine motif-containing protein MIX14
Cells are able to grow on a medium with oleate as a sole carbon source.
Cells are unable to grow on a medium with oleate as a sole carbon source.
In Bmut mutant; abolished ATPase activity of the PEX1-PEX6 AAA ATPase complex.
In PEX6pA2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes. In Amut mutant; abolished ATPase activity of the PEX1-PEX6 AAA ATPase complex.
In PEX6pB2; increased amount of peroxisome-bound PEX6. Results in accumulation of PEX5 on peroxisomal membranes.
Protein phosphatase PP2A regulatory subunit A
Putative guanine nucleotide-exchange factor SED4
Translation machinery-associated protein 46
Charge relay system; for autoendoproteolytic cleavage activity
Impairs processing of the proenzyme and increases protein degradation. Decreases activity.
Impairs processing of the proenzyme. Decreases activity.
In PSD1deltaIM; Mislocalizes to the mitochondrial matrix.
Increases protein degradation. Decreases activity.
Loss of autocatalytic processing and leads to protein degradation. Complete loss of activity. Growth on the fermentable carbon source galactose is moderately decreased.
Loss of autocatalytic processing and leads to protein degradation. Complete loss of activity. Growth on the fermentable carbon source galactose is severely decreased.
Mitochondrion; not cleaved when targeted to the endoplasmic reticulum
No processing of the proenzyme, complete loss of activity.
No processing of the proenzyme, complete loss of activity. Increases protein localization to the endoplasmic reticulum; when associated with A-210 and A-345.
No processing of the proenzyme. Increases protein localization to the endoplasmic reticulum; when associated with A-210 and A-463.
No processing of the proenzyme. Increases protein localization to the endoplasmic reticulum; when associated with A-345 and A-463.
Phosphatidylserine decarboxylase 1 alpha chain
Phosphatidylserine decarboxylase 1 beta chain
Phosphatidylserine decarboxylase proenzyme 1, mitochondrial
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
Significantly impairs processing of the proenzyme, retains some activity.
Abolishes tyrosine and tryptophan binding, resulting in loss of enzyme activity regulation by effectors.
Constitutively active. Unresponsive to tryptophan activation and tyrosine feedback inhibition.
Constitutively inactive. Unresponsive to tryptophan activation.
Decreases tyrosine and tryptophan binding, resulting in attenuated enzyme activity regulation by effectors.
Decreases tyrosine binding, resulting in loss of enzyme inhibition by tyrosine. Enhances activation by phenylalanine.
Severely decreases tyrosine and tryptophan binding, resulting in loss of enzyme activity regulation by effectors.
Regulator of Ty1 transposition protein 107
Genetic interactor of prohibitin 5, mitochondrial
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI15
Regulator of rDNA transcription protein 5
Actin cytoskeleton-regulatory complex protein PAN1
Multiple RNA-binding domain-containing protein 1
Decreases responsiveness to mannose and fructose.
In SNF3-1; constitutively signaling glucose receptor.
Eukaryotic translation initiation factor 5A-1
Impairs association to the ribosome and cell growth.
Impairs programmed ribosomal frameshifting.
In ts1159; temperature-sensitive growth phenotype and impairs programmed ribosomal frameshifting.
Lethal; when associated with D-118 and D-124.
Lethal; when associated with D-118 and N-120.
Temperature-sensitive growth phenotype. Lethal; when associated with L-83 and D-118 or with I-66 and D-118 or with D-118 and I-142 or with L-116 and D-118.
Temperature-sensitive growth phenotype. Lethal; when associated with Y-39 and D-118.
Temperature-sensitive growth phenotype; when associated with D-118. Lethal; when associated with Y-39 and D-118.
Temperature-sensitive growth phenotype; when associated with F-93.
Temperature-sensitive growth phenotype; when associated with L-116 or W-122 or F-140 or I-142. Lethal; when associated with Y-39 and I-66 or with Y-39 and L-83 or with Y-39 and L-116 or with Y-39 and F-140 or with Y-39 and I-142 or with N-120 and D-124.
Temperature-sensitive growth phenotype; when associated with V-118.
Temperature-sensitive growth phenotype; when associated with W-122.
Eukaryotic translation initiation factor 6
Restores interactions with PRP2 mutants.
Protein N-terminal and lysine N-methyltransferase EFM7
Abolishes interaction with BUB3. Benomyl-sensitive phenotype.
Abolishes interaction with CDC20. Benomyl-sensitive phenotype.
Spindle assembly checkpoint component MAD3
Converts to human ortholog residue: No effect.
Cytochrome c oxidase assembly protein COX15
Fails to rescue the heme a biosynthetic defect of a cox15delta mutant.
Mimicks pathogenic substituion found in human patients: Fails to rescue the heme a biosynthetic defect of a cox15delta mutant.
Structural maintenance of chromosomes protein 1
Probable NADPH:adrenodoxin oxidoreductase, mitochondrial
Phosphatidylserine decarboxylase 2 alpha chain
Phosphatidylserine decarboxylase 2 beta chain
Phosphatidylserine decarboxylase proenzyme 2
DNA-directed RNA polymerase I subunit RPA49
Loss of DNA binding; when associated with A-356.
Loss of DNA binding; when associated with A-358.
Uncharacterized membrane protein YCR023C
Completely inactivates the enzyme. Resistance to sordarin.
Protein phosphatase 2C homolog 7, mitochondrial
Decreases phosphatidic acid binding and impairs cardiolipin biosynthesis; when associated with A-62.
Decreases phosphatidic acid binding and impairs cardiolipin biosynthesis; when associated with A-65.
Decreases phosphatidic acid transfer activity and impairs cardiolipin biosynthesis.
Failure to complement the mitochondrial defects of UPS1-deficient cells.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-104.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-144.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-148 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-61 and E-148.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-58; E-61 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-33; E-61; E-148 and E-155.
Failure to complement the mitochondrial defects of UPS1-deficient cells; when associated with E-58; E-61; E-148 and E-155.
Impairs interaction with MDM35. Reduces ability to complement the mitochondrial defects of UPS1-deficient cells.
Nearly abolishes phosphatidic acid transfer activity.
Nearly abolishes phosphatidic acid transfer activity; when associated with E-155.
Nearly abolishes phosphatidic acid transfer activity; when associated with E-61.
No effect on phosphatidic acid transfer activity.
Strongly impairs interaction with MDM35. Failure to complement the mitochondrial defects of UPS1-deficient cells.
Probable pyridoxal 5'-phosphate synthase subunit SNO3
In mdm17; loss of function at 37 degrees Celsius.
Loss of function; most MGM1 processed to s-MGM1.
pH-response regulator protein palH/RIM21
Probable serine/threonine-protein kinase HSL1
Serine/threonine-protein phosphatase PP1-1
Gly-Pro-Asn (GPN)-loop; involved in dimer interface
Impairs heterodimer formation with NPA3/GPN1.
Carbamoyl-phosphate synthase arginine-specific large chain
U4/U6 snRNA-associated-splicing factor PRP24
U3 small nucleolar RNA-associated protein 25
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, H-210, D-364, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, D-364, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-371.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, G-371 and G-417.
In RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with V-186, H-210, D-364, G-371 and G-417.
23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
Abolishes interaction with VTA1 and reduces endosomal localization.
Vacuolar protein-sorting-associated protein 60
Maintenance of telomere capping protein 4
DNA-directed RNA polymerase II subunit RPB4
In A364a; suppressor of the cdc35-1 allele.
In sgt1-3; induces arrest of cell division in G2/M; when associated with L-99 and I-213.
In sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 I-213.
In sgt1-3; induces arrest of cell division in G2/M; when associated with P-31 and L-99.
In sgt1-5; induces arrest of cell division in G2/M; when associated with K-364.
In sgt1-5; induces arrest of cell division in G2/M; when associated with V-220.
Osmolarity two-component system protein SSK1
Uncharacterized membrane protein YDR090C
DNA replication complex GINS protein PSF1
In PSF1-1; temperature-sensitive mutant. Defective in DNA replication. Impaired chromatin binding of CDC45.
In SPO1-1; defective in sporulation at 34 degrees Celsius.
Inactivates the proteins ability to provide drug-resistance in vivo.
Inosine-5'-monophosphate dehydrogenase 2
In Dpl1CKSN_LLLL; impairs the formation of higher oder oligomeric complexes and consequently reduces catalytic activity.
Impaired palmitoylation and plasma membrane localization.
Abolishes mitochondrial localization and activity, but not cytoplasmic activity of the enzyme.
Target of rapamycin complex 2 subunit AVO1
eRF1 methyltransferase catalytic subunit MTQ2
Translation initiation factor IF-2, mitochondrial
Sporulation-specific wall maturation protein
Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
1-acylglycerol-3-phosphate O-acyltransferase ICT1
Constitutive activity; when associated with R-451.
Loss of activity. Constitutive activity; when associated with R-224.
Terminates elongation following the production of C22 instead of C-26; when associated with K-262.
Terminates elongation following the production of C22 instead of C-26; when associated with L-266.
Mitochondrial MRF1 N(5)-glutamine methyltransferase MTQ1
In PRP40-1 suppressor; affects SAR1 mRNA accumulation in U1-U4 mutant at 18 degrees Celsius.
Temperature sensitive growth at 36 degrees Celsius.
Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-304.
Abolishes phosphorylation; when associated with A-267; A-304; A-328 and A-397.
Abolishes phosphorylation; when associated with A-267; A-304; A-351 and A-397.
Abolishes phosphorylation; when associated with A-304; A-328; A-351 and A-397.
Anaphase-promoting complex subunit CDC27
In CDC27-633; G2/M cell cycle arrest at 35 degrees Celsius.
Uncharacterized membrane protein YDL218W
Ribonucleases P/MRP protein subunit POP7
Peroxisomal coenzyme A diphosphatase 1, peroxisomal
Galactose-1-phosphate uridylyltransferase
Truncated non-functional cytochrome oxidase 1
Eukaryotic translation initiation factor 2 subunit gamma
In SUI4; defective in ternary complex formation, correlating with a higher rate of dissociation from charged initiator-tRNA in the absence of GTP hydrolysis.
Increases the off-rate for GDP, without altering the apparent dissociation constant for Met-tRNAi(Met). Mimicks the function of the guanine nucleotide exchange factor eIF-2B.
Reduces the affinity of eIF-2 for Met-tRNAi(Met) without affecting the k(off) value for guanine nucleotides.
Abolishes endonuclease activity. In box3-2 / G1909; abolishes maturase and endonuclease activity; when associated with F-364.
Abolishes endonuclease activity. In box3-2 / G1909; abolishes maturase and endonuclease activity; when associated with N-393.
Abolishes maturase and endonuclease activity.
In M1282 / M4111; abolishes maturase and endonuclease activity.
In M4962; abolishes maturase and endonuclease activity.
In W404-3; abolishes maturase and endonuclease activity; when associated with M-395.
In W404-4; abolishes maturase and endonuclease activity; when associated with Y-379.
37S ribosomal protein S28, mitochondrial
Phosphoribosylglycinamide formyltransferase
Target of rapamycin complex 1 subunit KOG1
DNA-directed RNA polymerase III subunit RPC3
ATP-dependent RNA helicase MRH4, mitochondrial
Phosphatidylinositol N-acetylglucosaminyltransferase GPI2 subunit
Signal recognition particle subunit SEC65
Uncharacterized transcriptional regulatory protein YKL222C
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
5'-AMP-activated protein kinase subunit gamma
Decreases SNF1-activation efficiency; when associated with A-291 and E-293.
Decreases SNF1-activation efficiency; when associated with E-242 and A-291.
Decreases SNF1-activation efficiency; when associated with E-242 and E-293.
Reduces glucose inhibition of SNF1 and leads to resistance to 2-deoxyglucose.
Lead to defects in spindle pole body assembly.
Eukaryotic peptide chain release factor GTP-binding subunit
Uncharacterized membrane protein YNL320W
Putative redox protein FMP46, mitochondrial
Galactose/lactose metabolism regulatory protein GAL80
Increased stability following gamma-irradiation and loss of phosphorylation by DUN1. Causes lethality in rnr1-W688G strain which has a mutation in RNR1.
Ribonucleotide reductase inhibitor protein SML1
Succinate dehydrogenase assembly factor 3, mitochondrial
Nonsense-mediated mRNA decay factor EBS1
G1-specific transcriptional repressor WHI5
Transcription initiation factor TFIID subunit 13
Abrogates kinase activity and transcriptional repression.
Meiotic mRNA stability protein kinase SSN3
In SEC2-78; temperature-sensitive; causes mislocalization of the protein, displaying many cytoplasmic punctae.
Rab guanine nucleotide exchange factor SEC2
54S ribosomal protein IMG1, mitochondrial
Strongly reduces affinity for poly-U RNA ends.
U6 snRNA-associated Sm-like protein LSm3
Cytochrome b-c1 complex subunit 8, mitochondrial
Abolishes phosphatase activity and impairs cardiolipin biosynthesis.
Phosphatidylglycerophosphatase GEP4, mitochondrial
Glutathione-specific gamma-glutamylcyclotransferase
Loss of catalytic activity against glutathione.
Reduces enzymatic activities by almost 95%.
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-78.
Alters glycosyltransferase efficiency towards a subset of target proteins; when associated with S-81.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6
Eukaryotic translation initiation factor 5
In TIF5-12A; Abolishes binding to SUI3 and NIP1.
In TIF5-7A; Abolishes binding to SUI3 and NIP1.
Abolishes ability to monoubiquitinate histone H2B.
Abolishes interaction with LAS17 and causes severe mislocalization of the protein.
Has a constitutive higher activity in actin assembly.
25S rRNA (cytosine(2278)-C(5))-methyltransferase
Fails to catalyze the C-5 methylation of the C2278 residue.
Lethal. Blocks the separation of the enzyme from its RNA substrate.
Meiotic sister-chromatid recombination protein 3
Elongated telomeres and severe growth defects.
Altered inheritance of mitochondria protein 9, mitochondrial
Causes read-through of both a snoRNA gene terminator and the poly(A) site of a protein-coding gene.
In SEN1-1; gives rise to a temperature-sensitive mutant.
Upstream activation factor subunit UAF30
Vacuolar protein sorting-associated protein 17
Signal recognition particle subunit SRP68
Abolishes ATP binding, reducing ATPase activity by 95%. Displays 9800-fold increase in spontaneous mutation accumulation.
Abolishes heterodimer formation. Displays an increase in spontaneous mutation accumulation.
Defective in a mismatch repair assay. Abolishes heterodimer formation. Displays an increases spontaneous mutation accumulation.
Displays an increase in spontaneous mutation accumulation. Does not impair heterodimer formation.
In strain: EAY1066, EAY1068, M2-8, M7-8, M5-7, SK1, YJM145, YJM269, YJM320, YJM339 and YJM627; forms a non-functional heterodimer with PMS1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains.
Partially defective in a mismatch repair assay.
Reduces ATPase activity by 98%. Displays 3300-fold increase in spontaneous mutation accumulation.
Reduces DNA-binding and displays a 1700-fold increase in spontaneous mutation accumulation. Strongly reduces DNA-binding and displays 12000-fold increase in spontaneous mutation accumulation; when associated with E-273.
Reduces heterodimer formation. Displays an weak increase in spontaneous mutation accumulation.
Strongly reduces DNA-binding and displays 12000-fold increase in spontaneous mutation accumulation; when associated with E-274.
U3 small nucleolar ribonucleoprotein protein LCP5
Eukaryotic translation initiation factor 5A-2
Ribonucleoside-diphosphate reductase small chain 1
Structure-specific endonuclease subunit SLX4
Very long-chain fatty acid transport protein
Phosphatidylethanolamine N-methyltransferase
Citrinin resistance protein, mitochondrial
In DED1-120; impairs protein synthesis at 15 degrees Celsius; when associated with D-108.
In DED1-120; impairs protein synthesis at 15 degrees Celsius; when associated with D-494.
In DED1-18; impairs BMV RNA synthesis; when associated with D-317.
In DED1-18; impairs BMV RNA synthesis; when associated with M-237.
In DED1-199; impairs protein synthesis at 15 degrees Celsius.
Lethal in vivo and impairs ATPase and RNA-helicase activities in vitro.
Lethal in vivo and inhibits ATPase and helicase activities in vitro.
Reduces RNA-helicase activity about 2.5-fold in vitro.
Reduces RNA-helicase activity about 5-fold in vitro.
Reduces strongly ATPase activity and strand displacement activity.
Slow growth at 18 and 30 degrees Celsius and no growth at 16 degrees Celsius.
Slow growth at 18 and 36 degrees Celsius in vivo, and reduces RNA-helicase activity about 5-fold in vitro.
Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with Ala-158.
Alters DNA-binding activity and impairs MSH2-MSH3-mediated DNA mismatch repair; when associated with Ala-160.
Impairs MSH2-MSH3-mediated DNA mismatch repair.
Partially functional in a mismatch repair assay; when associated with 10-AA-11.
Partially functional in a mismatch repair assay; when associated with A-4.
Vacuolar protein sorting-associated protein 29
No effect. Slower growth and thermosensitivity; when associated with G-33 or G-52. Complete loss of function; when associated with G-33 and G-52 or G-52 and Y-102. Complete loss of function; when associated with G-33; G-52 and G-102.
No effect. Slower growth and thermosensitivity; when associated with G-52 or G-84. Complete loss of function; when associated with G-33; G-52 and G-84.
No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-33 and G-84. Complete loss of function; when associated with G-33; G-84 and G-102.
No effect. Slower growth and thermosensitivity; when associated with G-84. Complete loss of function; when associated with G-52 and G-84. Complete loss of function; when associated with G-52; G-84 and G-102.
Uncharacterized protein YGL041W-A, mitochondrial
Bifunctional purine biosynthetic protein ADE5,7
In RHO1-10; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with G-69.
In RHO1-10; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with P-165.
In RHO1-11; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with E-166.
In RHO1-11; temperature sensitive, severely decreases beta-1,3-glucan synthase activation; when associated with K-101.
In RHO1-2; temperature sensitive, fails to activate PKC1.
In RHO1-3; temperature sensitive, severely decreases beta-1,3-glucan synthase activation.
In RHO1-4; temperature sensitive, severely decreases beta-1,3-glucan synthase activation.
In RHO1-5; temperature sensitive, fails to activate PCK1.
Locks RHO1 in the GTP-bound form by abolishing GTP hydrolysis.
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Defect in snoRNA accumulation. Growth defect at 37 degrees Celsius.
Biogenesis of lysosome-related organelles complex 1 subunit SNN1
Monoisotopic mass with either 6 methylation modifications or 1 acetylation and 3 methylation modifications.
Eukaryotic translation initiation factor 1A
Type 2A phosphatase-associated protein 42
Defective in cullin neddylation protein 1
Ferric/cupric reductase transmembrane component 7
Mitochondrial inner membrane protease subunit 2
Reduces processing of CYB2 and presence of IMP1 and SOM1 in the IMP complex.
Reduces processing of cytochrome c1; no effect on presence of IMP1 and SOM1 in the IMP complex.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-114, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-114 and S-178.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-114 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-77, S-99, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-38, S-99, S-114, S-178 and S-192.
Loss of MIA40-dependent import into the mitochondrial intermembrane space; when associated with S-77, S-99, S-114, S-178 and S-192.
Mitochondrial intermembrane space cysteine motif-containing protein MIX23
Endoplasmic reticulum vesicle protein 25
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61
Expressed at lower levels. GTPase activity reduced.
Expressed at the same level as wild-type. GTPase and GDPase activities decreased more than 20-fold toward GTP and 3-fold for GDP.
Vacuolar protein sorting/targeting protein VPS10
Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with A-158.
Abolishes interaction with RSP5 and reduces HSE-mediated gene expression; when associated with Q-157.
Uncharacterized membrane protein YGL140C
Mitochondrial fusion and transport protein UGO1
Abolishes interaction with SEC62; defect in protein translocation.
Cytochrome c oxidase assembly protein COX20, mitochondrial
Transcription initiation factor IIA large subunit
General transcriptional corepressor TUP1
Loses ability to recognize arginine (R)-based ER localization signals in proteins. Recognition of C-terminal di-lysine signals present in proteins is unimpaired.
Thiamine biosynthesis regulatory protein
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)
Serine/threonine-protein phosphatase 4 catalytic subunit
Multifunctional methyltransferase subunit TRM112
Uncharacterized membrane protein YAL064W-B
Loss of DNA-binding; when associated with D-383.
Loss of DNA-binding; when associated with D-387.
Abolishes mitophagy and impairs interaction with ATG11.
Decreases mitophagy and impairs interaction with ATG11.
In PDC1-8; reduces catalytic activity to 10% but retains autoregulatory activity.
In afg2-18, drg1-18 or drg1-ts; temperature sensitive mutant. At the restrictive temperature of 37 degree Celsius, impaired growth. Severe loss of ATPase activity, homohexamer formation and 60S ribosomal subunit maturation. Prevents the release of shuttling proteins NOG1, RLP24 and ARX1 from cytoplasmic pre-60S particles resulting in their cytoplasmic accumulation. Loss of interaction with RLP24 and pre-60S particles. Restores growth and formation of 60S ribosomal subunit maturation but not catalytic activity or oligomerization; when associated with L-343.
In dgr1-sup*; moderate loss of catalytic activity. No growth defect. Restores growth and formation of 60S ribosomal subunit maturation but not catalytic activity or oligomerization; when associated with S-457.
In drg1-11; severe loss of ATPase activity. Severe loss of oligomerization. Resistant to diazaborine-mediated growth inhibition.
In drg1-1; slight loss of ATPase activity. No effect on affinity for ATP or oligomerization. Resistant to diazaborine-mediated growth inhibition. No defect in RLP24 release from pre-60S ribosomal particles in the absence or in the presence of diazaborine.
In drg1-3; resistant to diazaborine-mediated growth inhibition.
In drg1-4; resistant to diazaborine-mediated growth inhibition.
Increases ATPase activity and reduces affinity for ATP. Mild defect in oligomerization.
Increases ATPase activity. Loss of oligomerization.
Increases basal ATPase activity. Reduces RLP24-mediated activation. Does not affect interaction with RLP24. Prevents the release of shuttling proteins RLP24, NOG1 and MEX67 from cytoplasmic pre-60S ribosomal particles. Does not affect the interaction with pre-60S ribosomal particles.
Reduces basal and RLP24-dependent ATPase activity. Increases interaction with RLP24. Slightly reduces RLP24 release. Does not affect composition of pre-60S ribosomal particles or growth.
Probable S-adenosylmethionine-dependent methyltransferase CRG1
Does not complement a YME1 deletion mutant, for E541A stabilizes otherwise unstable COX2.
Does not complement a YME1 deletion mutant, for K327R no longer binds or degrades COX2. Probably has no ATPase activity.
Does not complement a YME1 deletion mutant, no longer binds or degrades COX2. Probably has no ATPase activity.
Does not complement a YME1 deletion mutant, retains 20% protease activity in vitro, binds an unfolded hybrid substrate protein.
Does not complement a YME1 deletion mutant.
Mitochondrial inner membrane i-AAA protease supercomplex subunit YME1
Partially complements a YME1 deletion mutant.
Cytosolic Fe-S cluster assembly factor NBP35
Loss fo function and disrupts heterotetramer formation.
Supports growth, albeit at a lower rate.
Altered inheritance of mitochondria protein 26, mitochondrial
Mediator of RNA polymerase II transcription subunit 8
Loss of aminopeptidase I precursor maturation and no more association with vacuole and punctate structures.
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI13
Chromatin modification-related protein EAF7
Abolishes formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
Interchain (with C-48 in TSA1); transient
Minor effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
No effect on formation of disulfide bond with TSA1 and reduction of cysteine-sulfinic acid.
Membrane-anchored lipid-binding protein LAM1
Vacuolar protein sorting-associated protein 62
Choline/ethanolaminephosphotransferase 1
External NADH-ubiquinone oxidoreductase 1, mitochondrial
N-terminal acetyltransferase B complex catalytic subunit NAT3
DNA-directed RNA polymerase III subunit RPC1
Vacuolar import and degradation protein 24
General transcriptional corepressor CYC8
Abolishes catalytic activity. Fails to localize to lipid droplet biogenesis sites on the ER.
Phospholipid:diacylglycerol acyltransferase
Cytochrome c oxidase assembly protein COX14
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-119 and A-121.
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-119 and A-123.
In Spo73AAA; disrupts localization to the prospore membrane; when associated with A-121 and A-123.
Transcription initiation factor TFIID subunit 3
60S ribosomal subunit assembly/export protein LOC1
Serine/threonine-protein phosphatase PP2A-2 catalytic subunit
tRNA-specific adenosine deaminase subunit TAD2
26S proteasome regulatory subunit 8 homolog
In allele sgs1-34; temperature-sensitive.
In allele sgs1-35; temperature-sensitive.
In allele sgs1-36; temperature-sensitive.
Diphosphoinositol polyphosphate phosphohydrolase DDP1
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase
Nonviable. Loss of catalytic activity. Loss of protein stability.
Nonviable. Loss of catalytic activity. No effect on protein stability and interaction with SPC3.
Nonviable. Loss of catalytic activity. No effect on protein stability.
Signal peptidase complex catalytic subunit SEC11
0.1% of catalytic activity. No specific ARS1 binding.
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
Prevents binding to COX17 mRNA and its rapid decay and increases affinity to HO mRNA, a PUF5 target.
Average mass with 1 acetylation and 1 methylation modification.
Probable glutathione-independent glyoxalase SNO4
Phosphopantothenoylcysteine decarboxylase subunit SIS2
Putative uncharacterized protein YCR095W-A
DNA-directed RNA polymerase III subunit RPC4
Putative uncharacterized protein YLR462W
Flavin prenyltransferase PAD1, mitochondrial
The measured mass is of the truncated protein without mitochondrial targeting sequence including the mass of an N-terminal hexahistidine tag, expressed in E.coli.
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM2
Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-746.
Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-748.
Polyphosphatidylinositol phosphatase INP53
Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-424.
Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-427.
Reduces hydrolysis of PtdIns(4)P; when associated with A-424 and A-427.
Changes the ribosome profile distribution of the protein and also increases its occurrence in the cytosolic fraction.
Changes the ribosome profile distribution of the protein.
tRNA A64-2'-O-ribosylphosphate transferase
U6 snRNA-associated Sm-like protein LSm8
Cytochrome c oxidase assembly protein COX11, mitochondrial
tRNA-splicing endonuclease subunit SEN15
10 kDa heat shock protein, mitochondrial
RNA polymerase I-specific transcription initiation factor RRN11
70% reduction in kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure.
Abolishes the stationary phase-dependent stimulation of DAG kinase activity.
Loss of kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure.
No kinase activity. Not temperature-sensitive for growth. Does not trigger nuclear membrane expansion.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate
Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule.
tRNA-splicing endonuclease subunit SEN34
Impairs phosphorylation; when associated with A-119; A-149 and A-178.
Impairs phosphorylation; when associated with A-119; A-149 and A-248.
Impairs phosphorylation; when associated with A-119; A-178 and A-248.
Impairs phosphorylation; when associated with A-149; A-178 and A-248.
Stabilizes VAC17 whose protein levels are about 10-fold higher than wild-type.
Mitochondrial oxaloacetate transport protein
Abolishes both iron-binding and enzyme activity.
Abolishes enzyme activity and impairs iron-binding.
Abolishes iron-binding, substrate-binding, and enzyme activity.
Abolishes substrate-binding and enzyme activity.
Abolishes substrate-binding, enzyme activity, and impairs iron-binding.
Serine/threonine-protein phosphatase PPQ
Lethal(2) giant larvae protein homolog SRO7
EH domain-containing and endocytosis protein 1
Vacuolar protein sorting-associated protein 3
Probable serine/threonine-protein kinase FMP48
Probable target of rapamycin complex 2 subunit BIT2
Transcription initiation factor TFIID subunit 9
Confers resistance to cycloheximide, an inhibitor of polypeptide elongation.
Monoisotopic mass with 2 methylation modifications.
Monoisotopic mass with N6-methyl-Lys-40 and N6-methyl-Lys-55.
With N6-methyl-Lys-40 and N6-methyl-Lys-55.
In YOS1-1; impairs interaction with YIP1, blocks ER-Golgi protein transport, and causes a severe growth defect at 37 degrees Celsius.
Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3.
Vacuolar import and degradation protein 22
Sugar utilization regulatory protein IMP2
Interferes with the hydrolysis of the covalent intermediate with DNA that is formed during the enzyme reaction. No effect on bleomycin sensitivity.
Strongly reduced release of the covalent intermediate with DNA that is formed during the enzyme reaction, leading to the accumulation of toxic adducts. No effect on bleomycin sensitivity.
In strain: CLIB 410, CLIB 413 and Sigma 1278B.
Vacuolar transporter chaperone complex subunit 1
Conserved oligomeric Golgi complex subunit 4
Serine hydroxymethyltransferase, mitochondrial
tRNA threonylcarbamoyladenosine dehydratase 2
Leads to a mild increase in the proportion of preanaphase spindles at the expense of elongated spindles.
Gly-cisPro motif, important for rejection of L-amino acids
Biogenesis of lysosome-related organelles complex 1 subunit BLI1
Reduces the Vmax 30-fold, but does not change the KM in a uridine hydrolase assay.
Translation machinery-associated protein 10
Spindle pole body-associated protein CIK1
37S ribosomal protein S19, mitochondrial
CDP-diacylglycerol--serine O-phosphatidyltransferase
Malonyl CoA-acyl carrier protein transacylase, mitochondrial
13 kDa ribonucleoprotein-associated protein
Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with A-84.
Impairs binding to U4 snRNA, but not U3 snoRNA, and causes pre rRNA processing defects and an accumulation of unspliced U3 snoRNA; when associated with L-81.
Impairs binding to U4 snRNA, but not U3 snoRNA. Causes pre-mRNA splicing and pre-rRNA processing defects.
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Serine/threonine-protein kinase Haspin homolog ALK1
Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial
Abolishes catalytic activity, but preserves its nonkinase transcription regulation functions.
Prevents correct localization of ASH1 mRNA.
Prevents correct localization of ASH1 mRNA; when associated with E-343.
Prevents correct localization of ASH1 mRNA; when associated with E-361.
In strain: ATCC 76625 / YPH499, MMW1-15 and MMW1-2.
In strain: ATCC 76625 / YPH499,MMW1-15, MMW1-2 and S288c / YPH1.
In strain: Bb32, M1-2A, M2-8, MMR2-5, MMW1-15h2, MMW1-2h2 and Sgu52F.
Abolishes short-term enzyme inactivation by glucose addition.
Reduces activity by 45%; when associated with L-220.
Reduces activity by 45%; when associated with R-216.
Low affinity vacuolar monovalent cation/H(+) antiporter
Vesicle-associated membrane protein-associated protein SCS22
Abrogates interaction with histone H3 and histone H4 and enhances transcriptional silencing. Abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with E-145 or E-147.
Abrogates interaction with histone H3 and histone H4 and enhances transcriptional silencing. Reduces acetylation of histone H3 on 'K-9' and 'K-56'; when associated with E-145 or E-147.
Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS.
Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with R-48; E-112 or E-147.
Abrogates stimulation of replication-independent chromatin assembly by the HIR complex and abrogates telomeric silencing.
Decreases histone H3/H4 binding affinity.
Enhances transcriptional silencing. Abrogates interaction with histone H3 and histone H4, abrogates transcriptional silencing, reduces transcriptional activation, reduces acetylation of histone H3 on 'K-9' and 'K-56' and enhances sensitivity to HU and MMS; when associated with R-48; A-112 or E-145.
Impairs interaction with histone H3 and RAD53 and enhances silencing at telomeres and mating-type loci.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with A-145.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with A-147.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with D-94.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to bleomycin, camptothecin, HU and MMS; when associated with D-96.
Reduces acetylation of histone H3 on 'K-56' and enhances sensitivity to camptothecin.
Reduces interaction with histone H3 and histone H4, enhances transcriptional silencing and reduces transcriptional activation.
Reduces transcriptional silencing; when associated with R-37.
Reduces transcriptional silencing; when associated with R-39.
Phosphopantothenate--cysteine ligase CAB2
Eukaryotic initiation factor 4F subunit p150
In TIF4631-213; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation.
Abolishes cleavage by ESP1 at position R-431; when associated with E-431.
Abolishes cleavage by ESP1 at position R-453.
Reduces cleavage by ESP1 at position R-431. Abolishes cleavage by ESP1 at position R-431; when associated with R-428.
Cytosolic iron-sulfur protein assembly protein 1
Impaired in cytosolic Fe/S protein assembly.
Almost complete loss of SAM binding. No effect on growth and ribosome biogenesis.
Loses its exclusive nucleolar localization and mislocalizes to the cytoplasm.
Loss of substrate rRNA binding. No effect on growth.
Ribosomal RNA small subunit methyltransferase NEP1
N-terminal acetyltransferase A complex subunit NAT1
Trehalose synthase complex regulatory subunit TPS3
Thiazolidine linkage to a ring-opened DNA abasic site
Induces collapsed, globular or grape-like mitochondria.
Induces collapsed, globular or grape-like mitochondria; when associated with A-225.
Induces collapsed, globular or grape-like mitochondria; when associated with A-354.
Asparagine synthetase [glutamine-hydrolyzing] 2
Reduced ATPase activity of the INO80 complex.
1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
Imidazole glycerol phosphate synthase hisHF
No activity. Uncoupling of glutaminase activity from the cyclase activity.
Abolishes ATPase and DNA helicase activities but not ATP-binding capacity.
General transcription and DNA repair factor IIH helicase subunit XPD
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Vacuolar protein-sorting-associated protein 25
Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial
Loss of catalytic activity, but not required for pre-rRNA processing.
25S rRNA (adenine(645)-N(1))-methyltransferase
Impaired methyltransferase activity and ribosomal 60S subunit biogenesis. Does not affect pre-rRNA cleavage at site A2 activity.
In RRP8-1; no effect. Synthetic lethal with GAR1 mutant allele lacking its N- and C-terminal glycine/arginine-rich (GAR) domains.
Uncharacterized membrane protein YFL051C
NEDD8-activating enzyme E1 regulatory subunit
Presequence translocated-associated motor subunit PAM17, mitochondrial
O-(5'-phospho-DNA)-tyrosine intermediate
Carbamoyl-phosphate synthase arginine-specific small chain
37S ribosomal protein NAM9, mitochondrial
In MNA6-1; causes temperature-dependent loss of the 15S rRNA.
In MNA6-2; causes temperature-dependent loss of the 15S rRNA.
In MNA6-3; causes temperature-dependent loss of the 15S rRNA.
In MNA6-4; causes temperature-dependent loss of the 15S rRNA.
In NAM9-1; suppressor for ocher mutations in mitochondrial DNA, possibly through decreasing the fidelity of translation.
Transcription initiation factor TFIID subunit 8
Succinate/fumarate mitochondrial transporter
Succinate dehydrogenase assembly factor 2, mitochondrial
2-acyl-1-lysophosphatidylinositol acyltransferase
Uncharacterized vacuolar membrane protein YNR061C
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at S-43; S-74; S-162; S-360; S-1179 and S-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-236 and A-305.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-127; A-157; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-127; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43, Ser-74, Ser-162, Ser-360, Ser-1179 and Ser-1183; when associated with A-67; A-157; A-163; A-231; A-236; A-305 and A-320.
In scc2-8A; mimics unphosphorylated form and leads to novel phosphorylation sites at Ser-43; S-74; S-162; S-360; S-1179 and Ser-1183; when associated with A-67; A-127; A-157; A-163; A-231; A-236 and A-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-127; E-157; E-163; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-236 and E-305. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-236 and E-305.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-236 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-236 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-231; E-305 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-231; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-163; E-236; E-305 and E-320. In scc2-2NE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-157; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-127; E-163; E-231; E-236; E-305 and E-320.
In scc2-8E; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU), and decreased stability of the MCD1 cohesin subunit in mitotic cells; when associated with E-67; E-157; E-163; E-231; E-236; E-305 and E-320.
In scc2-CE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU); when associated with E-1182.
In scc2-CE; mimics constitutive phosphorylation, retains normal SCC2-SCC4 interactions and chromatin association, but exhibits decreased viability, sensitivity to genotoxic agents methyl methanesulfonate (MMS) and hydroxyurea (HU); when associated with E-1185.
Mimics constitutive phosphorylation and causes inviability through protein instability.
Mitochondrial phosphate carrier protein 2
Peptidyl-prolyl cis-trans isomerase FPR2
Probable metabolite transport protein YBR241C
Abolishes interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B.
Abolishes interaction with histones H2A, H2B, H3 and H4.
Mildly decreases interaction with heterotetrameric histone H3 and H4 and abolishes interaction with dimeric histone H2A and H2B.
Minimal decrease of interaction with heterotetrameric histone H3 and H4 and with dimeric histone H2A and H2B.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-288 and A-291.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-250; A-291 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-248; A-288; A-291 and 332-A--A-342.
Strongly reduces affinity for dimeric histone H2A and H2B; when associated with A-250; A-288; A-291 and 332-A--A-342.
General negative regulator of transcription subunit 3
Prevents nuclear localization; when associated with A-82 and A-83.
Prevents nuclear localization; when associated with A-82 and A-85.
Prevents nuclear localization; when associated with A-83 and A-85.
Serine/threonine-protein phosphatase PP-Z2
Raises KM for malonyl-CoA by a factor of 15.
Raises KM for malonyl-CoA by a factor of 20.
Raises KM for malonyl-CoA by a factor of 70.
Uncharacterized membrane protein YOR034C-A
Cold-sensitive. Abolishes kinase activity. Delayed growth at early stationary phase. Shows no increase in CTD Ser-2 phosphorylation in the transition from rapid growth to stationary phase. Has compromised transcriptional activation of two stationary-phase genes CTT1 and SPI1.
Cold-sensitive. Sensitive to hydroxyurea and UV irradiation. Interferes with ATP-binding.
Phosphatidylinositol transfer protein PDR17
Checkpoint serine/threonine-protein kinase BUB1
Heterogeneous nuclear rnp K-like protein 2
Guanine nucleotide-binding protein subunit beta
DNA polymerase alpha catalytic subunit A
Increase in length of X' and Y' telomeres. No effect on telomere position effect. Reduced interaction with CDC13.
Increase in length of X' and Y' telomeres. Reduced interaction with CDC13.
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Genetic interactor of prohibitin 7, mitochondrial
Mannosyl phosphorylinositol ceramide synthase CSH1
In DSS4-1; a dominant suppressor of SEC4-8.
Impairs ATPase and 5-oxoprolinase activity.
Impairs ATPase and 5-oxoprolinase activity; when associated with A-497.
Impairs ATPase and 5-oxoprolinase activity; when associated with A-498.
ATPase synthesis protein 25, mitochondrial
Outward-rectifier potassium channel TOK1
Catabolic L-serine/threonine dehydratase
Dominant negative mutant; prevents the nuclear export of 60S ribosomal subunit in cytoplasm.
Impairs the nuclear export of 60S ribosomal subunit in cytoplasm.
Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-27.
Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-28.
Impairs interaction with UPS1 and UPS2; when associated with A-27 and A-28.
Mitochondrial distribution and morphology protein 35
Causes respiratory deficiency in absence of RSM28.
Meiosis-specific transcription factor NDT80
No effect on DNA-binding but strongly reduces progress through meiosis and sporulation.
Reduces DNA-binding by 50% and abolishes sporulation.
Reduces DNA-binding by 50% but does not abolish sporulation.
Reduces DNA-binding by 80% and abolishes sporulation.
Reduces DNA-binding by 80% and strongly reduces progress through meiosis and sporulation.
Reduces DNA-binding by 93% and abolishes sporulation.
Reduces DNA-binding by 95% and abolishes sporulation.
Reduces DNA-binding by 96% and abolishes sporulation.
Vacuolar protein sorting-associated protein 70
Glycosylphosphatidylinositol anchor biosynthesis protein 11
In SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius.
Non-structural maintenance of chromosomes element 1
Ketol-acid reductoisomerase, mitochondrial
In G5037; blocks formation of mature cytochrome b mRNA.
In M2011; leads to absence of functional cytochrome b, but does not affect splicing.
37S ribosomal protein MRP1, mitochondrial
54S ribosomal protein L36, mitochondrial
Ribonuclease P protein component, mitochondrial
Membrane-anchored lipid-binding protein SIP3
Potential protein lysine methyltransferase SET6
Chromatin structure-remodeling complex subunit RSC7
Ubiquinone biosynthesis O-methyltransferase, mitochondrial
Asparagine synthetase [glutamine-hydrolyzing] 1
Signal recognition particle subunit SRP54
Conserved oligomeric Golgi complex subunit 3
DNA repair and recombination protein RDH54
Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-182.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-181 and A-185.
In ALPHA2-3A; defective in binding DNA alone or in complex with MCM1, but binds DNA normally in complex with A1; when associated with A-182 and A-185.
Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1.
Reduces the ability of ALPHA2 to repress transcription, but binds normally to DNA and MCM1. Disrupts interaction with TUP1.
Reduces the ability of ALPHA2/MCM1 to repress a-specific genes.
Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6.
Vacuolar import and degradation protein 30
GPI ethanolamine phosphate transferase 3
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with P-556; G-887 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; G-887 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; P-556 and A-901.
In MCP1-4; impairs growth at 36 degrees Celsius; when associated with T-367; P-556 and G-887.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with R-198 and S-231.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with R-198 and T-411.
In MCP1-5; impairs growth at 36 degrees Celsius; when associated with S-231 and T-411.
Transcription initiation factor TFIID subunit 4
5-demethoxyubiquinone hydroxylase, mitochondrial
Lacks ubiquinone and accumulates the intermediate DMQH2, causing respiratory deficiency.
Loss of phosphorylation and increased levels of COQ6; when associated with S-20 and S-28.
Loss of phosphorylation and increased levels of COQ6; when associated with S-20 and T-32.
Loss of phosphorylation and increased levels of COQ6; when associated with S-28 and T-32.
Inactivates MET30 and prevents MET4 interaction; when associated with A-425 and A-467.
Inactivates MET30 and prevents MET4 interaction; when associated with A-425.
Inactivates MET30 and prevents MET4 interaction; when associated with D-386 and A-425.
Inactivates MET30 and prevents MET4 interaction; when associated with D-530 or D-386 and A-467.
Strongly reduces nuclear localization; inhibits interaction with SKP1/CBF3D.
In SRB2-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation.
Mediator of RNA polymerase II transcription subunit 20
Impairs SIN3-binding and gene repression activity.
Impairs meiotic genes expression and sporulation, reduces interaction with IME, and reduces phosphorylation.
Impairs meiotic genes expression and sporulation, reduces interactions with IME1 and RIM11, and reduces phosphorylation.
Impairs meiotic genes expression and sporulation, reduces the interaction with IME1, and abolishes phosphorylation.
Impairs meiotic genes expression, sporulation and interactions with IME1 and RIM11, and abolishes phosphorylation.
In upc2-1; increases aerobic sterol synthesis and uptake. Increases also aerobic expression of DAN1 and sensibility to NaCl and LiCl.
Peroxisomal membrane protein import receptor PEX19
54S ribosomal protein L28, mitochondrial
Impairs heterodimer formation with GPN2 and GPN3.
Impairs nuclear localization of RNAPII, but does not impair RNAPII binding.
Impairs nuclear localization of RNAPII. Completely abolishes RNAPII binding.
In strain: ABYS 60, DBVPG6044, SK1 and YPS128.
Severely affects activity, especially the second mannosylation step.
Cold-sensitive; reduces catalytic activity more than 20-fold at 14 degrees Celsius.
Reduces catalytic activity between 5- to 25-fold.
Serine palmitoyltransferase-regulating protein TSC3
Abolished phosphorylation by ATG1, leading to increased interaction with ATG8.
Abolishes the proteolytic activity and decreases autophagy.
Decreased autophagy caused by strongly reduced interaction with ATG8.
Does not affect autophagy. Slightly decreased interaction with ATG8.
Impaired autophagy caused by decreased delipdation of ATG8. Reduced recruitment to preautophagosomal structures.
Increased recruitmentto the phagophore assembly site.
Phospho-mimetic mutant; decreased integration with ATG8, leading to impaired decreased delipdation of ATG8.
Increases translation repression of ASH1 mRNA.
In LST8-1; abolishes repression of RTG2- and RTG1-RTG3-dependent gene expression.
In LST8-2; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-3; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-4; abolishes repression of RTG1-RTG3-dependent gene expression.
In LST8-5; abolishes repression of RTG1-RTG3-dependent gene expression.
Target of rapamycin complex subunit LST8
Coenzyme Q-binding protein COQ10, mitochondrial
Induces impairment in folding and loss of zinc-binding.
Loss of function due to severely affected folding and the presence of non-native disulfide bonds; loss of zinc-binding.
Mitochondrial import inner membrane translocase subunit TIM10
Disrupts binding to FFAT motif and causes OPI1 mislocalization.
Disrupts binding to FFAT motif; when associated with D-84.
Disrupts binding to FFAT motif; when associated with D-86.
Reduces binding to FFAT motif and impairs OPI1 function.
Vesicle-associated membrane protein-associated protein SCS2
Regulator of rDNA transcription protein 13
60S ribosome subunit biogenesis protein NOP8
KKRVK motif; Required for interaction with GLC7, endocytosis and actin cytoskeleton organization
Uncharacterized endoplasmic reticulum membrane protein YMR122W-A
Beta-glucosidase-like protein NCA3, mitochondrial
Probable serine/threonine-protein kinase KCC4
Probable ATP-dependent RNA helicase DHR2
Membrane-anchored lipid-binding protein LAM5
Strongly reduces E3 activity, but no effect on subcellular location.
Fatty acyl-CoA synthetase and RNA processing-associated kinase 1
54S ribosomal protein L35, mitochondrial
Centromere DNA-binding protein complex CBF3 subunit B
Putative uncharacterized protein YLR030W
Serine/threonine-protein kinase Haspin homolog ALK2
Structural maintenance of chromosomes protein 6
Unfolded protein response-inducible protein 1
Low-affinity potassium transport protein
Transcription initiation factor IIE subunit alpha
Putative mitochondrial cysteine synthase
54S ribosomal protein L32, mitochondrial
Abolishes splitting of stalled ribosomes. Defective activation of the ribosome quality control (RQC) pathway. Does not affect the association of the RQT complex with ribosomes.
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
18S rRNA (guanine(1575)-N(7))-methyltransferase
Can only partially restore the slow-growth phenotype and the random budding pattern of a null mutant.
Fails to ctalyze the N-7 methylation of the G1575 residue.
No effect on growth and budding pattern.
Inner membrane mitoribosome receptor MBA1, mitochondrial
Cytochrome c oxidase-assembly factor COX23, mitochondrial
Recessive mutant; phenotypically null. Eliminates the ability for self-association.
Increases the specific activity 2-fold and decreases sensitivity to CTP product inhibition 5-fold.
No effect on activity and product inhibition by CTP.
ERAD-associated E3 ubiquitin-protein ligase DOA10
In ERG20-2; 14-fold decrease in FPPS activity.
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR3
Sphingolipid long chain base-responsive protein LSP1
Does not alter the arsenite/proton exchange across the plasma membrane.
Impairs cell membrane localization, and leads to arsenite sensitivity.
Leads to ER retention and arsenite sensitivity.
Leads to ER retention, protein unstability and arsenite sensitivity.
Leads to complete loss of metalloid transport function.
Results in moderate reduction of arsenite transport capacities and sorting perturbations.
Transcription factor TFIIIB component B''
Increases readthrough in translation termination.
Ribosome-associated complex subunit SSZ1
Prevents association with ASH1 and IST2 mRNAs and leads to their mislocalization.
Prevents association with ASH1 and mRNA and leads to its mislocalization.
Uncharacterized membrane protein YDL180W
Putative 2-hydroxyacid dehydrogenase YGL185C
In saf1-1; impairs the protein stability.
In saf1-2; impairs the protein stability.
In saf1-3; impairs the protein stability.
In saf1-4; impairs the protein stability; when associated with K-263.
In saf1-4; impairs the protein stability; when associated with K-264.
In saf1-5; impairs the interaction with SKP1.
In saf1-6; impairs the interaction with SKP1.
In SSB1-D1: Reduces ribosome-binding to less than 50%.
In SSB1-D2: Reduces ribosome-binding to less than 50%.
In SSB1-L(BC): Reduces ribosome-binding to less than 50%.
Ribosome-associated molecular chaperone SSB1
Unable to hydrolyze ATP and moderately reduces ribosome binding.
Y' element ATP-dependent helicase protein 1 copy 6
Loss of autocatalytic cleavage; when associated with F-605.
Loss of autocatalytic cleavage; when associated with L-608.
Loss of autocatalytic cleavage; when associated with P-604.
Loss of autocatalytic cleavage; when associated with R-608.
2-deoxy-glucose resistant protein 1, mitochondrial
Glucosamine 6-phosphate N-acetyltransferase
Glucoamylase, intracellular sporulation-specific
Y' element ATP-dependent helicase YLL066C
Cytochrome b-c1 complex subunit 7, mitochondrial
37S ribosomal protein MRP2, mitochondrial
Initiation-specific alpha-1,6-mannosyltransferase
G1-specific transcription factors activator MSA1
Partially suppresses the respiratory defect of SHY1 mutants by increasing COX1 translation.
Abolished ATPase activity of the PEX1-PEX6 AAA ATPase complex.
In Amut mutant; abolished ATPase activity of the PEX1-PEX6 AAA ATPase complex.
In Bmut mutant; decreased ATPase activity of the PEX1-PEX6 AAA ATPase complex.
In PEX1pA2; decreased binding to PEX6. Results in accumulation of PEX5 on peroxisomal membranes.
In PEX1pB2; results in accumulation of PEX5 on peroxisomal membranes.
In MMM1-6; impairs both mtDNA maintenance and mitochondrial morpholgy.
Maintenance of mitochondrial morphology protein 1
Uncharacterized vacuolar membrane protein YGR125W
Cytochrome c lysine N-methyltransferase 1
Causes accumulation of pre-mRNA splicing intermediates.
Uncharacterized TLC domain-containing protein YPR114W
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19
Mannose 6-phosphate receptor-like protein 1
Ubiquitin domain-containing protein DSK2
In RMP1-6; temperature-sensitive phenotype. Defective in 5.8S rRNA processing.
In DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-661.
In DBF4-AAHH; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-664.
In DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-680.
Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands.
Weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands. In DBF4-CCAA; weakens interaction with ARS1 origin DNA and MCM2, but not other known ligands; when associated with A-674.
Exists mainly as monomer. Has no peroxiredoxin activity.
Exists mainly as monomer. Has no peroxiredoxin activity. Fails to protect glutamine synthetase against damage by DTT oxidation.
Interchain (with C-84 in SRX1); transient
Calcium/calmodulin-dependent protein kinase II
Alpha-ketoglutarate-dependent sulfonate dioxygenase
Peroxisome proliferation transcriptional regulator
Alters protein substrate specificity. Able to farnesylate Ras2 variants with a negatively charged C-terminal aspartate (CIID).
Alters protein substrate specificity. Able to farnesylate Ras2 variants with positively charged C-terminal amino acids (CIIR and CIIK).
Can substitute for PGGT-I beta subunit (CDC43) in vivo. Has increased activity to farnesylate a substrate for PGGT-I. On the other hand, the ability to farnesylate its own substrate is reduced, due to its increased affinity for PGGT-I protein substrates. Increases the kcat/Km value for PGGTase-I substrates about 20-fold.
Can substitute for PGGT-I beta subunit, by modulating the substrate specificity for the peptide substrate.
Can substitute for PGGT-I beta subunit, by modulating the substrate specificity for the peptide substrate. Increases the kcat/Km value for PGGTase-I substrates about 20-fold.
Farnesylates Ras-CII(S,M,L) at wild type levels but can no longer geranylgeranylate the RasCII(M,L) substrates.
Farnesylates Ras-CIIS and Ras-CIIM but not Ras-CIIL, and is not capable of geranylgeranylating the Ras-CII(M,L) substrates.
Farnesylates only Ras-CIIS and not Ras-CII(M,L), and it geranylgeranylates all three substrates as well or better than wild type.
In ram1-1; causes a defect in a-factor production.
In ram1-2; abolishes farnesyltransferase activity.
Protein farnesyltransferase subunit beta
Glycolytic genes transcriptional activator GCR2
37S ribosomal protein YMR-31, mitochondrial
3,4-dihydroxy-2-butanone 4-phosphate synthase
Not viable in aerobic conditions. Lethal; when associated with S-61.
Completely abolishes the phospholipase activity; when associated with A-88 and A-90.
Completely abolishes the phospholipase activity; when associated with A-90 and A-92.
Partially abolishes the phospholipase activity towards PS and PA. Completely abolishes the phospholipase activity; when associated with A-88 and A-92.
Abolishes E3 ubiquitin-protein ligase activity.
Sphingolipid long chain base-responsive protein PIL1
Altered inheritance rate of mitochondria protein 29
Mitochondrial nicotinamide adenine dinucleotide transporter 1
Decreases binding to ubiquitin and interaction with ELA1, and decreases polyubiquitination of RPO21 during transcription stress; when associated with 33-A--A-34.
Decreases binding to ubiquitin and interaction with ELA1, and decreases polyubiquitination of RPO21 during transcription stress; when associated with 54-A--A-55.
Osmosensing histidine protein kinase SLN1
DNA-directed RNA polymerase I subunit RPA12
Protoheme IX farnesyltransferase, mitochondrial
Probable electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Eukaryotic translation initiation factor 3 subunit I
Required for vacuolar localization. Mediates aggregation and vesicle formation in Cvt pathway
CDC48-associated ubiquitin-like/zinc finger protein 1
Does not affect activity but increases by twofold the amount of protein found in mixed disulfide with PDI1 or MPD2.
Impairs the capture of mixed-disulfide with PDI1 thereby blocking its function. Loss of activity; when associated with A-105.
In ERO1-1; induces defective folding of disulfide proteins.
In ERO1-2; induces defective folding of disulfide proteins.
Loss of activity. Prevents its reoxidation thereby blocking its function.
Loss of regulatory disulfide bond and strongly increased activity towards PDI; when associated with A-150.
Loss of regulatory disulfide bond and strongly increased activity towards PDI; when associated with A-295.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)
Causes impaired ribosomal translocation with an increased rate of -1 programmed ribosomal frameshift read-through during translation.
Causes resistance to fusidic acid and reduces sensitivity to sordarin.
Causes resistance to fusidic acid and sordarin.
N6,N6,N6-trimethyllysine; by EFM3; alternate
N6,N6-dimethyllysine; by EFM2; alternate
N6,N6-dimethyllysine; by EFM3; alternate
Prevents ADP-ribosylation of the diphthamide by diphtheria toxin.
Prevents post-translational modification of this residue to diphthamide. Results in a functional protein that is resistant to diphtheria toxin and sordarin.
Reduces sensitivity to fusidic acid and causes resistance to sordarin.
Reduces sensitivity to fusidic acid and sordarin.
Cytochrome B translational activator protein CBS2
Transcription factor IIIB 70 kDa subunit
Biogenesis of lysosome-related organelles complex 1 subunit BLS1
Transcriptional activator of sulfur metabolism MET4
GPI ethanolamine phosphate transferase 1
In mcd4-174; defective in endoplasmic reticulum-to-Golgi transport of GPI-anchored proteins.
Maltose fermentation regulatory protein MAL13
Repressor of RNA polymerase III transcription MAF1
Eukaryotic initiation factor 4F subunit p130
In TIF4632-233; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation.
BTB/POZ domain-containing protein YLR108C
Class E vacuolar protein-sorting machinery protein HSE1
Abolishes disulfide bond, leading to destabilization of the TIM23 complex.
Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-4.
Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-8.
Mitochondrial import inner membrane translocase subunit TIM17
Inositol phosphosphingolipids phospholipase C
Impairs protein folding and stability. Loss of function.
Causes hypersensitivity to DNA-damage-inducing agents.
Cytochrome b-c1 complex subunit 2, mitochondrial
Putative uncharacterized protein YEL076C-A
Putative uncharacterized protein YLR464W
In SEC16-1; ts accumulation of ER membranes.
In SEC16-2; ts accumulation of ER membranes.
In SEC16-3; ts accumulation of ER membranes.
In SEC16-4; ts accumulation of ER membranes.
Impairs ORC1-binding and leads to defective association with chromatin.
Putative uncharacterized protein YLR053C
C-1-tetrahydrofolate synthase, cytoplasmic
Cyclohydrolase activity is increased 2-fold. Dehydrogenase Km is increased 2-fold.
Cyclohydrolase activity is reduced 20-fold. Dehydrogenase Km is increased 7-fold.
Uncharacterized ABC transporter ATP-binding protein YDR061W
Uncharacterized membrane protein YNL143C
Mitochondrial protein import protein MAS5
In strain: YIIc12 haplotype Ha2 and YIIc17.
Hydrolyzes decanoate (C10) esters in contrast to wild-type. Active also on short chain esters from acetate (C2) to hexanoate (C6), but the catalytic efficiency is lower than that of wild-type.
Ribosome-associated molecular chaperone SSB2
3-methyl-2-oxobutanoate hydroxymethyltransferase
Mitochondrial group I intron splicing factor CCM1
RNA polymerase II nuclear localization protein IWR1
Regulator of Ty1 transposition protein 105
Decreases localization to nuclear envelope-vacuole contact sites.
Decreases phosphatidylinositol 3-phosphate binding. Vacuolar protein missorting. Abnormal actin cytoskeleton organization. Sensitive to the arginine analog canavanine.
Increases localization to nuclear envelope-vacuole contact sites. Synthetic lethal with MMM1. Normal vacuolar protein sorting and normal sporulation.
Increases localization to peroxisomes. Decreases localization to nuclear envelope-vacuole contact sites and endosomes.
Intermembrane lipid transfer protein VPS13
Normal vacuolar protein sorting and normal sporulation.
Synthetic lethal with MMM1. Normal vacuolar protein sorting and normal sporulation.
Vacuolar protein missorting. Synthetic lethal with MMM1. Normal sporulation.
Mediator of RNA polymerase II transcription subunit 4
Eliminates glycogen accumulation; when associated with F-230.
Eliminates glycogen accumulation; when associated with F-598.
Lipid droplet-associated triacylglycerol lipase
Temperature-sensitive. TFCIII-DNA complexes present a shift in their 5' border, generate slow-migrating TFIIIB-DNA complexes upon stripping TFIIIC by heparin or heat treatment, and allow initiation at downstream sites. TFIIIC-DNA complexes highly unstable at high temperatures.
Chromatin structure-remodeling complex protein RSC30
Shows a 4-fold reduced affinity for DNA.
Shows a strongly reduced affinity for linear and circular DNA.
Nitrosoguanidine resistance protein SNG1
Target of rapamycin complex 1 subunit TCO89
Sorting assembly machinery 37 kDa subunit
Glyceraldehyde-3-phosphate dehydrogenase 1
Putative uncharacterized protein YKR104W
Actin-related protein 2/3 complex subunit 4
Mitochondrial distribution and morphology protein 10
Decreases interaction with GCN1, but not with ACT1, and prevents inhibition of GCN2 activity in amino acid-starved cells; when associated with A-102.
Decreases interaction with GCN1, but not with ACT1, and prevents inhibition of GCN2 activity in amino acid-starved cells; when associated with A-106.
Slightly increases interaction with GCN1 and ACT1 and does not prevent inhibition of GCN2 activity in amino acid-starved cells; when associated with A-87.
Slightly increases interaction with GCN1 and ACT1 and does not prevent inhibition of GCN2 activity in amino acid-starved cells; when associated with A-90.
Abolishes DNA-binding and endonuclease activity.
Leads to camptothecin hypersensitivity and loss of function.
Leads to camptothecin hypersensitivity and loss of function; when associated with A-223.
Leads to camptothecin hypersensitivity and loss of function; when associated with A-225.
Leads to constitutive activation of the DNA repair function.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-278, and A-279. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-249 and A-279.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-278, and A-289. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-249 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-249, A-279, and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-278, A-279, and A-289. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-90, A-279 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-73, A-75 and A-76. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-73, A-249, A-279 and A-289.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-73, A-75 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-72, A-75, A-76 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-73, A-75, A-76 and A-90.
Reduces DNA damage-induced phosphorylation; when associated with A-73, A-75, A-76 and A-90. Abolishes DNA damage-induced phosphorylation and function in DNA repair; when associated with A-90, A-249, A-279 and A-289.
Uncharacterized membrane protein YMR253C
ABC transporter ATP-binding protein/permease PDR18
Abolishes acetyltransferase activity; but not chromatid cohesion activity.
Impairs interaction with ATG8 and decreases subsequent reticulophagy.
In ASI13-1; dominant active and constitutively nuclear localized transcription factor.
In STP1-102; prevents proteolytic processing. Activates transcription also in its unprocessed form; when associated with 27-AASGAA-32.
In STP1-133; impairs cytoplasmic retention, resulting in a dominant active transcription factor. Activates transcription also in its unprocessed form; when associated with A-66.
Y' element ATP-dependent helicase YIL177C
Impairs histone deacetylase activity and transcription repression.
Confers interaction with methylated histone H3 'K-4'.
Disrupts interaction with methylated histone H3 'K-4'; abrogates histone acetylation activity of SLIK.
No effect on interaction with methylated histone H3 'K-4'.
No interaction with methylated histone H3 'K-4'.
N-terminal acetyltransferase A complex catalytic subunit ARD1
Attenuation of the response to Ras proteins.
Transmembrane protein 184 homolog YKR051W
Phosphoribosylformylglycinamidine synthase
Vacuolar protein sorting-associated protein 16
Leads to mislocalization into the cytoplasm.
Strongly reduces phosphorylation by CK2.
Cytoplasmic dynein intermediate light chain DYN3
Nucleolar pre-ribosomal-associated protein 2
Altered inheritance of mitochondria protein 6
No effect in conferring Congo red resistance; when associated with A-130.
No effect in conferring Congo red resistance; when associated with A-208.
Reduced interaction with WW domain of RSP5. No interaction with WW domain of RSP5; when in association with 82-QA-83.
Reduced interaction with WW domain of RSP5. No interaction with WW domain of RSP5; when in association with A-105.
Serine/threonine-protein phosphatase PP2A-like PPG1
General negative regulator of transcription subunit 1
Mutants do not survive after treatment with DNA-damaging agent MMS.
Mutants show a growth defect after treatment with DNA-damaging agent MMS.
Mutants show a slight growth defect after treatment with DNA-damaging agent MMS.
Mutants show a strong growth defect after treatment with DNA-damaging agent MMS.
Mutants show a very strong growth defect after treatment with DNA-damaging agent MMS.
Mutants show no effect after treatment with DNA-damaging agent MMS.
tRNA(Thr) (cytosine(32)-N(3))-methyltransferase
In TSV115; thermosensitive. Level of TFIIIC and its affinity for tDNA reduced. tDNA binding activity very sensitive to mild heat treatments, and TFIIIC-DNA interaction inhibited at moderate salt concentrations.
Transcription factor tau 138 kDa subunit
Putative uncharacterized protein YBR201C-A
ABC1 family protein YPL109C, mitochondrial
Putative uncharacterized transporter YOL162W
U3 small nucleolar ribonucleoprotein protein IMP3
3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
Mitochondrial import inner membrane translocase subunit TIM8
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Lethal; abolishes interaction with OST1 and WBP1.
Lethal; greatly reduces amount of OST1 in complex.
Lethal; reduces glycosylation; greatly reduces amount of OST1 in complex.
Temperature sensitive and staurosporine sensitive.
Temperature-sensitive; reduces amount of OST1 in complex.
Uncharacterized helicase-like protein YBL111C
Y' element ATP-dependent helicase YLL067C
Ribonucleoside-diphosphate reductase small chain 2
Truncated transposon Ty1-A Gag-Pol polyprotein
Abolished translocase activity, characterized by impaired ability to extract tail-anchored proteins from membranes.
Abolished translocase activity, characterized by impaired ability to unfold and extract tail-anchored proteins from membranes.
Does not impair ability to extract tail-anchored proteins from membranes. Does not affect formation of a homohexamer.
Impaired ability to extract tail-anchored proteins from membranes.
Impaired ability to extract tail-anchored proteins from membranes. Does not affect formation of a homohexamer.
Impaired ability to extract tail-anchored proteins from membranes. Impaired formation of a homohexamer.
Impaired formation of a homohexamer. Impaired ability to extract tail-anchored proteins from membranes.
Loss of ATPase activity. Does not affect formation of a homohexamer.
Outer mitochondrial transmembrane helix translocase
Phosphoadenosine phosphosulfate reductase
Uncharacterized globin-like protein YNL234W
Histone-lysine N-methyltransferase, H3 lysine-36 specific
Reduces dramatically histone methyltransferase activity toward nucleosomes.
Decreases interaction with tubulin heterodimer.
Disrupts interaction with tubulin heterodimer.
Disrupts interaction with tubulin heterodimer. Impairs polymerase activity under microtubule stress.
Disrupts interaction with tubulin heterodimer. No effect on polymerase activity under normal conditions but impairs polymerase activity under microtubule stress.
Impairs polymerase activity under microtubule stress.
No effect on polymerase activity under normal conditions but impairs polymerase activity under microtubule stress.
Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Coiled-coil domain-containing protein YLR146W-A
Glucose transport transcription regulator RGT1
Leads to reduced colony size when FKH2 is deleted.
Leads to accumulation of 35S and 27SB pre-rRNAs and a net 40S ribosomal subunit defect.
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Aromatic/aminoadipate aminotransferase 1
Mannan polymerase II complex ANP1 subunit
Uncharacterized membrane protein YOR365C
Putative uncharacterized protein YOL024W
RNA polymerase I-specific transcription initiation factor RRN10
Ribonucleases P/MRP protein subunit POP3
Abolishes DNA polymerase activity and increases UV-induced mutations.
Abolishes POL30-binding; when associated with A-627.
Abolishes POL30-binding; when associated with A-628.
Decreases efficiency of nucleotide incorporation.
Genetic interactor of prohibitins 3, mitochondrial
Putative alanyl-tRNA editing protein alaX
Does not completely restore mitochondrial DNA-dependent respiratory chain activities in respiration-defective mutant cells.
Mitochondrial translation optimization protein 1
Altered inheritance of mitochondria protein 4
Conserved oligomeric Golgi complex subunit 7
Mediator of RNA polymerase II transcription subunit 5
Vacuolar transporter chaperone 3 complex subunit 3
Putative cystathionine gamma-synthase YLL058W
In YIP1-12; abolishes binding to YPT1 and YPT31.
In YIP1-18; abolishes binding to YIF1, YPT1 and YPT31.
In YIP1-19; lethal; abolishes binding to YPT1 and YPT31, and reduces binding to YIF1.
In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with E-129.
In YIP1-1; blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius; when associated with L-114.
In YIP1-2; abolishes binding to YPT1 and YPT31, reduces binding to YIF1, blocks ER-Golgi protein transport, and causes a severe growth defect at 36 degrees Celsius.
In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with G-70.
In YIP1-41; lethal; abolishes binding to YIF1, YPT1 and YPT31. In YIP1-40; temperature sensitive; reduces binding to YPT31, and inhibits ER vesicle budding; when associated with A-130.
In YIP1-42; temperature sensitive; abolishes binding to YPT1 and YPT31, and inhibits ER vesicle budding.
In YIP1-44; lethal; when associated with K-70.
In YIP1-4; temperature sensitive; reduces binding to YIF1, YPT1 and YPT31. In YIP1-44; lethal; when associated with E-130.
In YIP1-6; lethal; reduces binding to YIF1.
Altered inheritance of mitochondria protein 44
Mitochondrial import inner membrane translocase subunit TIM23
54S ribosomal protein L23, mitochondrial
Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity.
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Impairs interaction with histones H3 and H4; when associated with F-187.
Impairs interaction with histones H3 and H4; when associated with F-354.
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited
Putative uncharacterized protein YLR157W-D
Uncharacterized membrane protein YNR062C
Geranylgeranyl pyrophosphate synthase BTS1
Monomer and homodimer. Monomer; when associated with G-7.
Mostly monomer. Exclusively monomer; when associated with G-8. Reduces enzyme activity 1000-fold.
No effect. Monomer; when associated with G-8.
Reduced affinity for isopentenyl diphosphate (IPP).
Uncharacterized membrane protein YML131W
Cytochrome c oxidase subunit 26, mitochondrial
Glycylpeptide N-tetradecanoyltransferase
In NMT1-181; temperature-sensitive with myristic acid auxotrophy. Reduces affinity for myristoyl-CoA.
In NMT1-72; temperature-sensitive mutant with myristic acid auxotrophy.
Moderately reduces affinity for myristoyl-CoA, but not for substrate.
Moderately reduces affinity for substrate, but not for myristoyl-CoA.
Reduces affinity for both substrate and myristoyl-CoA.
Reduces affinity for myristoyl-CoA, but not for substrate.
Reduces affinity for substrate, but not for myristoyl-CoA.
Reduces chemical transformation rate 400-fold.
Reduces the chemical transformation rate; when associated with A-170.
Reduces the chemical transformation rate; when associated with A-171.
Reduces the chemical transformation rate; when associated with A-205.
Reduces the chemical transformation rate; when associated with L-169.
Mitochondrial mRNA-processing protein COX24
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-311.
Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-312.
Abolishes copper resistance but not cadmium resistance; when associated with A-298.
Abolishes copper resistance but not cadmium resistance; when associated with A-300.
Confers localization to the plasma membrane and cadmium transport function.
Ubiquitin carboxyl-terminal hydrolase 14
Ribonucleases P/MRP protein subunit POP8
37S ribosomal protein MRP51, mitochondrial
Suppresses defects in the 5'UTLs of COX2 and COX3 mitochondrial mRNAs.
The measured mass includes the mass of an N-terminal hexahistidine tag, expressed in E.coli.
Oxygen-dependent coproporphyrinogen-III oxidase
Mediator of RNA polymerase II transcription subunit 9
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-310.
Disrupts the interaction with YTM1 and cannot sustain growth; when associated with R-104 or A-123.
In ytm1-1; abrogates binding to ERB1 and impairs 27S pre-rRNA processing and 66S pre-ribosome maturation; when associated with D-398.
In ytm1-1; abrogates binding to ERB1 and impairs 27S pre-rRNA processing and 66S pre-ribosome maturation; when associated with N-442.
Weakens the interaction with ERB1, leading to mostly ribosome-unbound YTM1 and defects in 60S maturation. Disrupts the interaction with YTM1 and cannot sustain growth; when associated with E-310.
Putative uncharacterized protein YDL057W
Serine/arginine (SR)-type shuttling mRNA binding protein GBP2
Shifts localization of the protein to the cytoplasm at steady state.
In TEF2-1; strongly reduces translation fidelity by increasing the frequency of frameshifting and amino acid misincorporation.
In TEF2-2; strongly reduces translation fidelity by increasing the frequency of frameshifting and amino acid misincorporation.
Increases KM for GTP to 10.3 mM and reduces translation fidelity; when associated with T-152.
Increases KM for GTP to 13.1 mM and reduces translation fidelity. Confers hyperresistance to canavanine.
Increases KM for GTP to 4.2 mM. Preferres XTP over GTP as substrate.
Increases KM for GTP to 6.0 mM and reduces translation fidelity. Increases Km for GTP to 10.3 mM and reduces translation fidelity; when associated with E-156.
N6,N6-dimethyllysine; by EFM4; alternate
N6,N6-dimethyllysine; by EFM7; alternate
RNA polymerase II subunit A C-terminal domain phosphatase
Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a A-511 mutation.
Impaired homodimerization and ATP-binding. Homodimerization and ATP-binding are recovered when it heterodimerizes with an ATG7 molecule with a R-524 mutation.
Instead of the formation of an intermediate complex with a thiol ester bond between ATG7 (E1-like enzyme) and ATG8 (substrate) or between ATG7 and ATG12 (substrate), a stable complex with an O-ester bond is formed. No more formation of ATG12-ATG5 conjugate.
Loss of interaction with ATG8 and ATG12 and no more formation of ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.
Loss of interaction with ATG8 and ATG12, and no more ATG12-ATG5 conjugate. Defect in Cvt pathway and autophagy.
Loss of interaction with ATG8; when associated with A-466 and A-490.
Loss of interaction with ATG8; when associated with A-466 and F-486.
Loss of interaction with ATG8; when associated with F-486 and A-490.
Ubiquitin-like modifier-activating enzyme ATG7
Loss of function. Localizes correctly to the mitochondrion, but reduces the steady-state level of the protein in the mitochondrial fraction.
Loss of function. Localizes correctly to the mitochondrion, but severely reduces the steady-state level of the protein in the mitochondrial fraction.
ARF guanine-nucleotide exchange factor 1
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in URM1)
Interchain (with C-31 in TRX2); transient
No effect on tert-butyl hydroperoxide consumption.
Inner membrane assembly complex subunit 22
Topoisomerase I damage affected protein 8
Mitochondrial morphogenesis protein SLD7
Vacuolar protein sorting-associated protein IST1
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Protein arginine N-methyltransferase SFM1
Strongly diminishes the catalytic activity towards both known substrates, aconitate and homoaconitate.
Decreases interaction with elongator complex subunits ELP2 and ELP5. Resistance to zymocin. Sensitive to thermal stress and caffeine. Normal sensitivity to diphtheria toxin and interaction with ATS1/KTI13.
Little or no effect on interaction with ATS1/KTI13.
Resistance to zymocin. Sensitive to thermal stress, diphtheria toxin, and caffeine.
Cysteine-rich and transmembrane domain-containing protein YDL012C
Potassium-activated aldehyde dehydrogenase, mitochondrial
Impairs the interaction with APM2 and its recruitment to membranes, and leads to sertraline sensitivity.
Sensitive to high expression protein 9, mitochondrial
Abolishes function; no effect on interaction with MSH4.
Medium-chain fatty acid ethyl ester synthase/esterase 2
Does not rescue the lethal phenotype of the deletion mutant.
Partially rescues the lethal phenotype of the deletion mutant.
Rescues the lethal phenotype of the deletion mutant.
Dolichyl-phosphate-mannose--protein mannosyltransferase 3
U3 small nucleolar RNA-associated protein 10
Probable phospholipid-transporting ATPase DRS2
Glycerol-3-phosphate dehydrogenase, mitochondrial
Arginine metabolism regulation protein II
N-linked (GlcNAc...) asparagine; partial
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13, YIIc12 and YIIc17.
Transcription initiation factor TFIID subunit 10
Decreases the interaction between the mediator complex and GCN4. Decreases transcription of GCN4-dependent targets. Sensitive to amino acid starvation.
Decreases transcription of GCN4-dependent targets. Decreases recruitment of the mediator complex to the upstream activating sequence (UAS) of amino-acid starvation responsive genes. Sensitive to amino acid starvation.
Mediator of RNA polymerase II transcription subunit 15
Essential for maintenance of the cell wall protein 1
Abolishes binding to and packaging of cargo protein BET1.
Methylthioribulose-1-phosphate dehydratase
Impairs the folding and stability of the protein.
Partially impairs the folding and stability of the protein.
Disrupts interaction with POL1 and FUN12.
Disrupts interaction with POL1 but not FUN12.
Increase in length of X' and Y' telomeres. Disrupts interaction with POL1 but not FUN12.
Histone deacetylase complex subunit CTI6
Impairs growth under iron-limiting conditions.
Acetolactate synthase catalytic subunit, mitochondrial
Trafficking protein particle complex III-specific subunit 85
U3 small nucleolar RNA-associated protein 20
DNA-directed RNA polymerases I and III subunit RPAC2
Beta-glucan synthesis-associated protein KRE6
General transcription and DNA repair factor IIH subunit TFB1
DNA-directed RNA polymerase II subunit RPB3
Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.
Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.
In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.
U3 small nucleolar RNA-associated protein 18
Restores water permeability; when associated with P-255.
Restores water permeability; when associated with V-121.
In fis1-L80P; no interaction with MDV1; mitochondrial fission is blocked; DNM1 assembly at punctate structures normal as in wild-type.
Signal recognition particle subunit SRP21
Reduces kcat over 100000-fold. Abolishes of the proton exchange reaction that initiates the enzymatic reaction.
5-aminolevulinate synthase, mitochondrial
Lethal in combination with a MCX1 disruption.
Ribosome-releasing factor 2, mitochondrial
Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-827.
Impairs deubiquitination activity and binding to BRO1; when associated with A-826 and A-829.
Impairs deubiquitination activity and binding to BRO1; when associated with A-827 and A-829.
Impairs deubiquitination activity and protein sorting into the MVB pathway.
Dolichyl-phosphate-mannose--protein mannosyltransferase 4
Decreases activity. Inositol auxotrophy.
Uncharacterized membrane protein YMR155W
NAD-dependent malic enzyme, mitochondrial
ATPase-stabilizing factor 9 kDa, mitochondrial
Does not affect autophagy; when associated with A-1086.
Does not affect autophagy; when associated with A-249.
Leads to a severely reduced activity of autophagy and a dispersed localization in the cytoplasm.
Phospho-mimetic mutant; does not affect autophagy; when associated with D-1086.
Phospho-mimetic mutant; does not affect autophagy; when associated with D-249.
Putative serine/threonine-protein kinase YPL150W
Leads to 30 percent decreased oxidoreductase activity.
Leads to decreased oxidoreductase activity.
Biogenesis of lysosome-related organelles complex 1 subunit CNL1
DNA polymerase zeta processivity subunit
Leads to partial block in enzymatic activity.
Vacuolar serine-type carboxypeptidase ATG42
Mediator of RNA polymerase II transcription subunit 1
Putative uncharacterized transporter YOL163W
Impairs the import of mitochondrial carrier proteins into mitochondria.
In tim9-19; impairs the import of mitochondrial carrier proteins into mitochondria.
In tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with A-40.
In tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with P-60.
Mitochondrial import inner membrane translocase subunit TIM9
Probable kynurenine--oxoglutarate transaminase BNA3
Does not affect function; when associated with A-12; E-17 and D-22.
Does not affect function; when associated with D-16 and D-19.
Does not affect function; when associated with D-16 and D-20.
Does not affect function; when associated with D-19 and D-20.
Does not affect function; when associated with R-11; A-12 and D-22.
Does not affect function; when associated with R-11; A-12 and E-17.
Does not affect function; when associated with R-11; E-17 and D-22.
Mitochondrial distribution and morphology protein 12
Temperature- and formamide-sensitive; abolishes cleavage and polyadenylation activity.
Temperature- and formamide-sensitive; impaired polyadenylation activity.
RNA-binding suppressor of PAS kinase protein 1
Putative uncharacterized protein YDL007C-A
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1063 and A-1282.
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1063 and A-1286.
Disrupts dimer formation and elongator complex formation but does not affect binding to tRNA; when associated with A-1282 and A-1286.
Does not affect elongator complex activity.
Does not affect elongator complex activity. Loss of elongator complex activity, reduced levels of mcm5U and ncm5U on tRNA and reduced interaction with HRR25 but no effect on elongator complex assembly; when associated with A-1198.
Does not affect elongator complex activity. Loss of elongator complex activity, reduced levels of mcm5U and ncm5U on tRNA and reduced interaction with HRR25 but no effect on elongator complex assembly; when associated with A-1202.
Loss of elongator complex activity. Abolishes binding to tRNA. Does not disrupt elongator complex assembly but decreases association of ELP1 with ELP5 and KTI12. Does not affect ELP1 self-association or association with ELP3. Does not affect the predominantly cytoplasmic localization.
Loss of phosphorylation at this site. Loss of elongator complex activity. Almost complete loss of mcm5U and ncm5U on tRNA. Does not affect elongator complex assembly. Enhances interaction with HRR25 and KTI12.
Phosphomimetic mutant. Does not affect elongator complex activity.
Slightly reduced levels of mcm5U and ncm5U on tRNA; when associated with E-1198.
Slightly reduced levels of mcm5U and ncm5U on tRNA; when associated with E-1202.
Some loss of elongator complex activity.
Eukaryotic translation initiation factor 2 subunit alpha
Inhibits derepression of GCN4 expression in amino acid, purine, and glucose-starved cells.
Weakly impairs derepression of GCN4 expression in amino acid-starved cells.
Impairs degradation of UBC7-substrates, but does not prevent protein degradation.
Prevents polyubiquitin chain attachment and protein degradation.
Loss of phosphorylation. Greatly increases catalytic activity and promotes homodimerization.
Phosphomimetic mutant. Inhibits catalytic activity and homodimerization.
Mitochondrial FAD-linked sulfhydryl oxidase ERV1
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Probable 1,3-beta-glucanosyltransferase GAS3
Sporulation-specific glucan 1,3-beta-glucosidase
Confers UV-radiation sensitivity; when associated with F-48 and F-87.
Confers UV-radiation sensitivity; when associated with F-48 and S-88.
Confers UV-radiation sensitivity; when associated with F-87 and S-88.
Impairs ubiquitin conjugation, DNA double-strand brakes formation during meiosis and histone H3-K79 methylation.
Actin-related protein 2/3 complex subunit 1
Succinate-semialdehyde dehydrogenase [NADP(+)]
Target of rapamycin complex 2 subunit AVO2
In THI6-1; both enzyme activities greatly decreased.
In THI6-2; both enzyme activities greatly decreased.
In THI6-3; possesses TMP-PPASE activity only.
Proton acceptor; for hydroxyethylthiazole kinase activity
Thiamine biosynthetic bifunctional enzyme
Does not enable copper chaperone-independent activation.
Enables copper chaperone-independent activation; when associated with A-143 or with S-143.
Enables copper chaperone-independent activation; when associated with A-145 or with L-145.
Interchain (with C-229 in CCS1); in linked form
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22
Reduces thermal stability and enzyme activity; when associated with A-526 or C-526. No effect; when associated with D-526 or Q-526.
Reduces thermal stability and enzyme activity; when associated with D-457.
Putative uncharacterized protein YGR079W
U6 snRNA-associated Sm-like protein LSm7
Mitochondrial inner membrane magnesium transporter MFM1
Chromosome segregation in meiosis protein 2
In pcl1-2A; stabilizes PCL1; when associated with A-39.
In pcl1-2A; stabilizes PCL1; when associated with A-43.
Peptidyl-prolyl cis-trans isomerase CPR4
Abrogates exonuclease activity and impairs termination of transcription by RNA polymerase II.
Causes mislocalization to the cytoplasm and suppresses the requirement for XRN1 function.
Causes mislocalization to the cytoplasm; when associated with A-534 and A-535.
Causes mislocalization to the cytoplasm; when associated with A-534 and A-537.
Causes mislocalization to the cytoplasm; when associated with A-535 and A-537.
In allele TAP1-1; activates transcription of the promoter-defective yeast SUP4 tRNA(Tyr) allele SUP4A53T61.
Suppresses the requirement for XRN1 function.
U3 small nucleolar RNA-associated protein 9
Abolished ability to mediate peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal; when associated with H-106.
Abolished ability to mediate peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal; when associated with R-61.
Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins.
Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins; when associated with A-344.
Does not prevent peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal, such as 3-oxoacyl-CoA thiolase.
Does not prevent peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal, such as 3-oxoacyl-CoA thiolase. Decreased formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins; when associated with A-364.
Mannan polymerase I complex VAN1 subunit
Causes partial retention of the protein in the endoplasmic reticulum.
Causes the protein to be retained in the endoplasmic reticulum (ER) and degraded via ER-associated degradation.
Oligomycin resistance ATP-dependent permease YOR1
Dihydrosphingosine 1-phosphate phosphatase LCB3
Impairs dihydrosphingosine 1-phosphate phosphatase activity.
Loss of activity. No activation of VPS34 kinase activity.
Modest effect on activity and normal CPY sorting.
No myristoylation, but still membrane-association and normal activity.
Translation termination inhibitor protein ITT1
Uncharacterized deoxyribonuclease YMR262W
ISWI chromatin-remodeling complex ATPase ISW2
Pheromone-processing carboxypeptidase KEX1
Putative uncharacterized oxidoreductase YGL039W
Alpha1-proteinase inhibitor-degradation deficient protein 37
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21
Uncharacterized membrane protein YLR046C
Leads to defects in A1 and A2 processing.
DSC E3 ubiquitin ligase complex subunit 2
Probable vacuolar amino acid transporter YPQ3
U6 snRNA-associated Sm-like protein LSm2
Increased protein secretion most likely due to reduced catalytic activity. Cells are larger and clump together.
Trafficking protein particle complex subunit 31
Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial
UDP-N-acetylglucosamine transporter YEA4
Putative uncharacterized protein YHL042W
Nuclear membrane organization protein APQ12
Restriction of telomere capping protein 5
Abolishes the binding of phosphatidylinositol 3-phosphate and the cytoplasm to vacuole transport.
Decreases mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant.
Lethal in double RPS19A/RPS19B mutant, considerable decrease in 18S rRNA production.
Loss of mature 18S rRNA, protein binds 20S pre-RNA complex poorly. Lethal in double RPS19A/RPS19B mutant.
Loss of mature 18S rRNA, protein doesn't bind 20S pre-RNA complex. Lethal in double RPS19A/RPS19B mutant.
Partial loss of function; decreased 18S rRNA, decreases binding to 20S pre-rRNA complex, slow growth in double RPS19A/RPS19B mutant.
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Beta-glucan synthesis-associated protein SKN1
DNA-directed RNA polymerase III subunit RPC10
Translation machinery-associated protein 22
Serine/threonine-protein kinase PTK1/STK1
Suppresses postrecruitment-defective SPT15/TBP alleles.
Spindle pole body-associated protein VIK1
Chromatin structure-remodeling complex protein RSC3
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-149 in ATG5)
Impairs conjugation to ATG5 and autophagic activity.
Strong decrease of conjugation with ATG5.
Induces a strong reduction in ability to stimulate mtHSP70 activity but does not affect import of proteins into mitochondrial matrix.
Mitochondrial import inner membrane translocase subunit TIM14
Temperature-sensitive mutant that impairs heterodimerization with PAM16 and import of proteins into mitochondrial matrix when incubated at 37 degrees Celsius.
Putative uncharacterized protein YBR013C
2-fold increase in steepness of voltage dependence.
2.5-fold reduction in steepness of voltage dependence.
Mitochondrial outer membrane protein porin 1
Dominant active allele that abolishes intrinsic GTPase activity.
Dominant negative allele unable to undergo the GTP-induced conformational change.
Guanine nucleotide-binding protein alpha-2 subunit
Locks GPA2 in its activated GTP-bound form and abrogates the negative control by RGS2.
54S ribosomal protein L40, mitochondrial
Uncharacterized transcriptional regulatory protein YNR063W
Trehalose synthase complex regulatory subunit TSL1
Altered inheritance of mitochondria protein 24, mitochondrial
Probable 26S proteasome regulatory subunit p27
3-oxoacyl-[acyl-carrier-protein] reductase
Abolishes ubiquitin binding. Defective activation of the ribosome quality control (RQC) pathway.
Geranylgeranyl transferase type-2 subunit beta
Dissociation of the elongator complex following assembly. Abolished interaction with KTI11 and KTI12.
Dissociation of the elongator complex following assembly. Loss of elongator complex activity; when associated with A-118.
Dissociation of the elongator complex following assembly. Loss of elongator complex activity; when associated with A-121.
Does not affect interaction with KTI11 and KTI12. Loss of elongator complex activity.
Eliminates iron contents; when associated with S-108 and S-118.
Eliminates iron contents; when associated with S-108 and S-121.
Eliminates iron contents; when associated with S-118 and S-121.
Impaired tRNA wobble uridine modification.
Leads to low activity and reduced substrate affinity.
Does not affect formation of a ternary complex composed of PEX21 and PEX7 along with PTS2-containing cargo proteins.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Chromatin structure-remodeling complex subunit RSC2
In dpm18; defective in plasmid maintenance.
In dpm3; defective in plasmid maintenance.
Decreased activation of Hsp90 ATPase activity with a small reduction in Hsp90 binding affinity.
Decreases activation of Hsp90 ATPase activity and substantially reduces Hsp90 binding affinity.
Decreases activation of Hsp90 ATPase activity with a small reduction in Hsp90 binding affinity.
No effect on Hsp90 ATPase activity but reduces Hsp90 binding affinity.
Isocitrate dehydrogenase [NADP], mitochondrial
Guanine nucleotide-exchange factor SEC12
ATP-dependent RNA helicase SUV3, mitochondrial
In suv3-1; probably excised introns are less efficiently released.
U3 small nucleolar RNA-associated protein 14
Reduces level of VLP formation and maturation.
Nascent polypeptide-associated complex subunit beta-1
Endosomal/prevacuolar sodium/hydrogen exchanger
Impairs partially resistance to osmotic stress and hygromycin.
Impairs protein-trafficking to the vacuole.
Impairs resistance to osmotic stress and hygromycin, and blocks protein-trafficking to the vacuole.
Impairs resistance to osmotic stress and hygromycin.
In got1-1; loss of function. ER-Golgi transport defect when associated with lack of SFT2.
Uncharacterized mitochondrial outer membrane protein YPR098C
Probable phospholipase YOR022C, mitochondrial
Nucleolar pre-ribosomal-associated protein 1
Uncharacterized membrane protein YHL071W
U3 small nucleolar RNA-associated protein 12
Eukaryotic translation initiation factor 2A
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase
Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype.
Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype.
DNA-directed RNA polymerase I subunit RPA43
NADPH-dependent alpha-keto amide reductase
E3 ubiquitin-protein ligase substrate receptor MMS22
Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1
Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial
Reduced ATP-dependent RNA helicase activity.
Strongly reduced unwinding of U4/U6 snRNA.
In strain: CBS 1171 and in allele SED1-1, SED1-2, SED1-3, SED1-4, SED1-5, SED1-6 and SED1-7.
Protein-S-isoprenylcysteine O-methyltransferase
Probable glutathione-independent glyoxalase HSP33
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-181.
Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-185.
Defective in ESCRT-I cargo sorting; when associated with D-102.
Defective in ESCRT-I cargo sorting; when associated with D-88.
Defective in ESCRT-I cargo sorting; when associated with D-92.
Defective in ESCRT-I cargo sorting; when associated with D-95.
Defective in ESCRT-I cargo sorting; when associated with D-99.
Peptide methionine sulfoxide reductase 2
Probable phospholipid-transporting ATPase DNF3
Abolishes interaction with TIF5; when associated with 16-K--K-23 and 49-K--K-56.
Abolishes interaction with TIF5; when associated with 16-K--K-23 and 82-K--K-89.
Abolishes interaction with TIF5; when associated with 49-K--K-56 and 82-K--K-89.
Eukaryotic translation initiation factor 2 subunit beta
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54
ADP-ribosylation factor GTPase-activating protein GLO3
Silencing boundary-establishment protein FUB1
U1 small nuclear ribonucleoprotein component SNU71
In VMA4-1; is rapidly degraded at 37 degrees Celsius.
Increases ATPase activity 2.1-fold by increasing Vmax for ATP hydrolysis 2-fold. Reduces ATPase activity by 90% and produces structurally aberrant V-ATPase complexes; when associated with A-233.
Reduces ATPase activity by 74%. Reduces ATPase activity by 90% and produces structurally aberrant V-ATPase complexes; when associated with A-202.
Reduces ATPase activity by 88%. Prevents assembly of V1 subunits at the membrane.
Chromatin structure-remodeling complex subunit RSC4
Probable transcriptional regulatory protein HAH1
SCF E3 ubiquitin ligase complex F-box protein GRR1
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
Abolishes both DNA glycosylase and AP lyase activity.
Diminishes both DNA glycosylase and AP lyase activity.
Destabilizes; not detectable in anaphase cells; spindle becomes thin and fragile.
NADPH-dependent 1-acyldihydroxyacetone phosphate reductase
Ferric reductase transmembrane component 6
RNA 3'-terminal phosphate cyclase-like protein
Vacuolar protein sorting-associated protein 1
Transcription initiation factor TFIID subunit 1
Probable glutamine amidotransferase DUG3
In gpi10-1; accumulate M2, an abnormal GPI-anchor intermediate due to the absence of third mannose.
Vacuolar cation-chloride cotransporter 1
Impairs nuclear export; when associated with A-12.
Impairs nuclear export; when associated with A-15.
In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with A-148.
In PAB1-148; greatly reduces poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro; when associated with N-151.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-166 and Q-259.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-166 and Q-362.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with A-79; Q-259 and Q-362.
In PAB1-14; fails to bind poly(U), but not poly(A) RNA; when associated with Q-166; Q-259 and Q-362.
In PAB1-157; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-16; reduces affinity for oligo(A) about 100-fold, impairs poly(A)-dependent translation, but still interacts with eIF4G; when associated with V-170. In PAB1-15; fails to bind RNA; when associated with V-170; V-263 and V-366.
In PAB1-175; greatly reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-180; abolishes poly(A)-dependent translation and greatly reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.
In PAB1-184; abolishes poly(A)-dependent translation and moderately reduces stimulation of cap-dependent translation in vitro. Impairs interaction with eIF4G.
In PAB1-193; moderately reduces stimulation of cap-dependent translation in vitro.
In PAB1-199; moderately reduces poly(A)-dependent translation and stimulation of cap-dependent translation in vitro.
In PAB1-6; selectively reduces poly(A) RNA binding. In PAB1-16; reduces affinity for oligo(A) about 100-fold, impairs poly(A)-dependent translation, but still interacts with eIF4G; when associated with V-83. In PAB1-15; fails to bind RNA; when associated with V-83; V-263 and V-366.
In PAB1-7. In PAB1-15; fails to bind RNA; when associated with V-83; V-170 and V-366.
In PAB1-8; selectively reduces poly(U) RNA binding. In PAB1-15; fails to bind RNA; when associated with V-83; V-170 and V-263.
Polyadenylate-binding protein, cytoplasmic and nuclear
Magnesium-activated aldehyde dehydrogenase, cytosolic
Mitochondrial import inner membrane translocase subunit TIM18
ER degradation-enhancing alpha-mannosidase-like protein 1
In CNS1-1; disrupts interaction with Hsp90, temperature-sensitive defect impairing Hsp90-dependent function.
In CNS1-2; disrupts interaction with Hsp90, temperature-sensitive defect impairing Hsp90-dependent function.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-260 and G-324.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-260 and S-330.
In CNS1-3; temperature-sensitive defect impairing Hsp90-dependent function; when associated with G-324 and S-330.
Abolishes ATPase activity, leading to secretion of resident ER proteins.
Prevents dimerization; when associated with S-285.
Prevents dimerization; when associated with S-288.
Putative uncharacterized protein YAL037C-A
Uncharacterized membrane protein YPR071W
Mitochondrial import receptor subunit TOM22
Is rapidly degraded and disrupts stable ATPase assembly.
Putative uncharacterized protein YLR031W
Vacuolar protein sorting-associated protein 68
Cleavage of initiator methionine, acetylation of Ala-2 by NatA, slightly reduced acetylation levels but no significant effect on endogenous stability or ability to degrade CPY*.
Degradation in the endoplasmic reticulum protein 1
In der1-2; impairs the ability to degrade misfolded proteins.
N-terminus not predicted to be acetylated. Strongly decreases endogenous stability.
Predicted to lead to acetylation by NatC. No effect on endogenous stability or ability to degrade CPY*.
Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase 2
Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs).
Abolishes autophosphorylation; when associated with A-257 and A-357.
Abolishes autophosphorylation; when associated with A-47 and A-257.
Abolishes autophosphorylation; when associated with A-47 and A-357.
Abolishes iron-sulfur-binding; when associated with A-519; A-768 and A-771. Impaired nuclease and ATPase activities; when associated with A-771.
Abolishes iron-sulfur-binding; when associated with A-519; A-768 and A-777. Impaired nuclease and ATPase activities; when associated with A-777.
Abolishes iron-sulfur-binding; when associated with A-519; A-771 and A-777. Impaired nuclease and ATPase activities; when associated with A-519.
Abolishes iron-sulfur-binding; when associated with A-768; A-771 and A-777. Impaired nuclease and ATPase activities; when associated with A-768.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-17 and A-237.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-4 and A-17.
Abolishes phosphorylation by CDK1, leading to a poor recruitment at double-strand. break (DSB) sites following DNA damage; when associated with A-4 and A-237.
DNA replication ATP-dependent helicase/nuclease DNA2
In dna2-1; temperature-sensitive mutant unable to grow at 37 degrees Celsius, probably due to abolition of iron-sulfur-binding.
Mimics phosphorylation; restores nuclear localization and recruitment at double-strand. break (DSB) sites following DNA damage; when associated with D-17.
Mimics phosphorylation; restores nuclear localization and recruitment at double-strand. break (DSB) sites following DNA damage; when associated with D-237.
Nuclease dead mutant. No helicase activity when the 5'-end of the substrate is blocked.
37S ribosomal protein PET123, mitochondrial
Glycerophosphocholine phosphodiesterase GDE1
U3 small nucleolar RNA-associated protein 7
Y' element ATP-dependent helicase YJL225C
ER-localized J domain-containing protein 5
Phosphatidyl-N-methylethanolamine N-methyltransferase
Chromatin-remodeling complex subunit IES6
Reduced enzyme activity; abolishes stimulation of ATPase activity upon DNA binding.
Strongly reduced enzyme activity; abolishes stimulation of ATPase activity upon DNA binding.
Abolishes cell surface localization. Abolishes acid trehalase activity.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-2 and R-27.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-2 and R-37.
Abolishes its ubiquitination; when associated with R-27 and R-37. Normal protein targeting to vacuolar lumen; when associated with R-27 and R-37.
Protein mislocalized to the vacuolar membrane in a small proportion of cells.
Glycerol-3-phosphate O-acyltransferase 2
In GAT1-TTA1; abolishes both GAT and DHAPAT activities.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-664 and A-668.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-664 and A-671.
In GPT2-3A; increases the enzyme activity of GPT2; when associated with A-668 and A-671.
54S ribosomal protein IMG2, mitochondrial
Translation machinery-associated protein 20
Putative cystathionine gamma-synthase YML082W
Dihydrolipoyl dehydrogenase, mitochondrial
In allele MUS81-DD; abrogates endonuclease activity.
Abolishes import into mitochondrion; when associated with S-57.
Abolishes import into mitochondrion; when associated with S-61.
Abolishes import into mitochondrion; when associated with S-73.
Abolishes import into mitochondrion; when associated with S-77.
Mitochondrial import inner membrane translocase subunit TIM13
In dsl1-22; strong Golgi-ER retrograde transport defect.
Loss of interaction with RET1; when associated with A-413.
Viable and no effect. Lethal and loss of interaction with RET2 and reduced interaction with RET1; when associated with A-413.
Viable and reduced interaction with RET2, strong Golgi-ER retrograde transport defect. Loss of interaction with RET2; when associated with A-455. Lethal and loss of interactions with RET1 and RET2; when associated with A-425.
56 kDa U1 small nuclear ribonucleoprotein component
Conserved oligomeric Golgi complex subunit 6
Mediator of RNA polymerase II transcription subunit 10
Probable phospholipid translocase non-catalytic subunit CRF1
Prevents interaction with GLC7 and reduces nuclear levels of GLC7 and SDS22.
Thiamine-repressible mitochondrial transport protein THI74
Mitochondrial 2-oxodicarboxylate carrier 2
Abolishes cleavage by ESP1; when associated with D-268.
Abolishes first cleavage by ESP1. Abolishes all cleavage by ESP1; when associated with D-180.
Reduces phosphorylation. Abolishes phosphorylation; when associated with A-175.
Reduces phosphorylation. Abolishes phosphorylation; when associated with A-263.
37S ribosomal protein S18, mitochondrial
Putative nucleotide-sugar transporter YMD8
Greatly reduces actin-binding and actin-bundling activity.
Moderately reduces actin-binding and actin-bundling activity.
Can only cleave double-flap structures with a 3' 1-nucleotide tail. Has no exonuclease activity.
Deficient in double and single flap endonuclease cleavage and exonucleolytic cleavage.
Deficient in exonuclease and gap endonuclease activities, but retains almost all of its flap endonuclease activity.
Reduces interaction with POL30 more than 100 fold.
In SUA5-1; suppresses a translation initiation defect in a CYC1 allele at an aberrant ATG codon.
Intracellular protein transport protein USO1
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Abolishes cyclic nucleotide phosphodiesterase activity.
Reduces strongly cyclic nucleotide phosphodiesterase activity.
Putative uncharacterized protein YJR120W
DNA-directed RNA polymerase III subunit RPC5
Loss of complementation of the suppression phenotype.
Putative uncharacterized protein YLR157W-E
Diminishes phosphorylation; 75-80% loss in kinase activity; no effect on survival.
No effect on phosphorylation; no effect on kinase activity.
Decreased S-adenosyl-L-methionine-binding.
Decreased aminocarboxypropyltransferase activity; when associated with A-60 and A-131.
Decreased aminocarboxypropyltransferase activity; when associated with A-60 and A-65.
Decreased aminocarboxypropyltransferase activity; when associated with A-65 and A-131.
Does not affect S-adenosyl-L-methionine-binding.
Reduced aminocarboxypropyltransferase activity.
DNA replication complex GINS protein SLD5
In sld5-12; temperature-sensitive mutant. Defective in DNA replication.
In sld5-13; temperature-sensitive mutant. Defective in DNA replication.
In sld5-2; temperature-sensitive mutant. Defective in DNA replication.
In sld5-8; temperature-sensitive mutant; in association with P-21. Defective in DNA replication.
In sld5-8; temperature-sensitive mutant; in association with P-66. Defective in DNA replication.
Putative uncharacterized protein YLR415C, mitochondrial
Abolishes thiocarboxylation and function in 2-Thiolation of tRNA.
3-ketodihydrosphingosine reductase TSC10
Serine/threonine-protein kinase/endoribonuclease IRE1
Nuclear distribution protein nudE homolog 1
Vacuolar protein sorting-associated protein 8
Y' element ATP-dependent helicase protein 1 copy 4
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3
Causes defects in ATP-dependent dissociation of MutS alpha from mismatch DNA and in interactions between MutS alpha and MutL alpha. Defective in MMR, but not in NHTR.
Causes strong defects in MutS alpha mismatch binding. Defective in MMR, but not in NHTR.
Disrupts MutS alpha ATPase activity, but does not affect ATP binding or interactions with MutL alpha. Defective in MMR, but not in NHTR.
Has no defect in mismatch DNA binding, but lacks ATP-induced conformational change. Defective in MMR and in NHTR.
Impairs ATP binding; reduces catalytic activity 1.6-fold for ATP hydrolysis. Has a dominant negative mutator effect.
Reduces catalytic activity 50-fold for ATP hydrolysis.
Loss of activity. No effect on interaction with LCB1.
Putative transcriptional regulatory protein YJL206C
54S ribosomal protein L51, mitochondrial
Y' element ATP-dependent helicase protein 1 copy 2
Loss of catalytic activity. Loss of ATP binding. Reduced survival and growth in response to reactive oxygen species.
Protein adenylyltransferase SelO, mitochondrial
Abolishes phosphorylation; when associated with A-44; A-59; A-103; A-115 and A-406.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-115.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-103 and A-406.
Abolishes phosphorylation; when associated with A-44; A-59; A-95; A-115 and A-406.
Abolishes phosphorylation; when associated with A-44; A-95; A-103; A-115 and A-406.
Abolishes phosphorylation; when associated with A-59; A-95; A-103; A-115 and A-406.
Anaphase-promoting complex subunit CDC16
In CDC16-183; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC16-1; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC16-264; G2/M cell cycle arrest at 36 degrees Celsius.
Abolishes SDH activity and complex assembly.
Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-260.
Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-261.
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Nuclear localization sequence-binding protein
Peptidyl-prolyl cis-trans isomerase CYP7
37S ribosomal protein MRP4, mitochondrial
Mediator of RNA polymerase II transcription subunit 19
Impairs BIR1 degradation and death-promoting activity.
26S proteasome regulatory subunit 6B homolog
Chromatin structure-remodeling complex subunit SFH1
Abolishes fusion function, MDM30-binding and MDM30-dependent ubiquitination, but not localization to mitochondrial outer membrane.
Abolishes fusion function, MDM30-binding and MDM30-dependent ubiquitination. No effect on localization or interaction with UGO1.
Abolishes fusion function; when associated with Ala-501.
Abolishes fusion function; when associated with Ala-504.
Abolishes respiratory growth. Restores respiratory growth; when associated with D-464.
Abolishes respiratory growth. Restores respiratory growth; when associated with D-780.
Abolishes respiratory growth. Restores respiratory growth; when associated with K-335.
Impairs GTP Hydrolysis and abolishes fusion function.
Leads to an unusual intermediate mitochondrial morphology described as disorganized tubules in which mitochondria are tubular, distorted, less branched, and poorly distributed throughout the cell.
No effect. Restores respiratory growth; when associated with H-523.
54S ribosomal protein L24, mitochondrial
Ubiquitin carboxyl-terminal hydrolase 11
In oligomycin-resistant mutant and cross-resistance to venturicidin.
In strain: DS400/A3, DS401 and oligomycin-resistant mutant.
Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47.
Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57.
Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47.
Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57.
D-lactate dehydrogenase [cytochrome] 1, mitochondrial
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX5
Leads to loss of SLX5 recruitment to ub-hotspots.
Putative uncharacterized protein YPR202W
Increased recombination centers protein 19
Ubiquitin carboxyl-terminal hydrolase 12
Abolishes catalytic activity; when associated with E-382.
Abolishes catalytic activity; when associated with R-248.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl; when associated with A-386.
Accumulates ubiquinone biosynthesis intermediates lacking the C5-ring hydroxyl; when associated with D-388.
Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial
Peroxisomal hydratase-dehydrogenase-epimerase
Inositol auxotrophy. Impaired ER morphology and aberrant lipid droplet (LD) budding.
Inositol auxotrophy. Impaired ER morphology with the apparition of clumps of ER membrane.
No defect in inositol biosynthesis. Aberrant lipid droplet (LD) budding.
Temperature sensitive. Inositol auxotroph at 37 degrees Celsius, but partially supports growth on inositol at 25 degrees Celsius. Impaired ER morphology and aberrant lipid droplet (LD) budding.
ATP-dependent clpX-like chaperone, mitochondrial
Abrogates unfolding activity of unfoldase. Mildly increased ATPase activity. Reduces ALA synthesis.
Mitochondrial 2-oxodicarboxylate carrier 1
Putative aryl-alcohol dehydrogenase AAD14
In TIF1-1; no growth at 36 degrees Celsius.
Increases about 3-fold ATP binding but reduces about 2-fold ATPase activity in vitro.
Lethal and dominant negative if overexpressed in vivo. Impairs ATP hydrolysis, RNA-helicase and translation activity in vitro.
Lethal and dominant negative if overexpressed.
Lethal in vivo and reduces ATP binding and ATPase activity in vitro.
Reduces ATP binding and ATPase activity in vitro.
Slow growth in vivo and reduces ATP binding and ATPase activity in vitro.
Abolishes helicase activity resulting in elongated telomeres; binds normally to DNA substrates.
In PIF1-m1; loss of mitochondrial function.
2-oxoglutarate dehydrogenase, mitochondrial
DNA-(apurinic or apyrimidinic site) endonuclease 2
Cytochrome b-c1 complex subunit 6, mitochondrial
Abolishes interaction with FIP1; when associated with R-485.
Abolishes interaction with FIP1; when associated with Y-489.
Reduces rate of adenylyltransfer about four-fold.
Reduces rate of adenylyltransfer by half.
Slightly reduced rate of adenylyltransfer.
Abolishes interaction with BBC1 and VRP1.
Bypasses the requirement of SHE4 for proper actin cytoskeleton polarization.
In MYO5-1; temperature sensitive loss of function.
Leads to a depolarized actin cytoskeleton and a strong defect in the capacity to internalize STE2.
No growth defect, but leads to a partially depolarized actin cytoskeleton. Accelerates the constitutive internalization of STE2.
Cell wall integrity and stress response component 2
Uncharacterized vacuolar membrane protein YNL115C
Hydroxyacylglutathione hydrolase, mitochondrial
37S ribosomal protein S24, mitochondrial
No effect on NTP-binding and hydrolysis. Resistant to DNA damage.
Reduces NTP-binding and hydrolysis. Shows DNA damage sensitivity.
Ubiquitin carboxyl-terminal hydrolase 13
Late endosome and vacuole interface protein 11
TPR repeat-containing protein associated with Hsp90
Sporulation-specific mitogen-activated protein kinase SMK1
Decreases quinone reductase activity. Little effect on complex assembly.
Succinate dehydrogenase [ubiquinone] cytochrome b subunit, mitochondrial
Structural maintenance of chromosomes protein 4
Uncharacterized membrane protein YNL033W
Mitochondrial genome maintenance protein MGM101
Mediator of RNA polymerase II transcription subunit 6
Inactivates catalytic activity and leads to substrate retention.
Abolishes ubiquitin chain elongation. No effect on thioester formation at the active site.
Lowers rate of ubiquitin chain elongation. No effect on thioester formation at the active site.
Strongly reduces MMS2 binding and interferes with error-free DNA repair.
In SUB2-100; no growth at 37 degrees Celsius.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; G-83 and M-142.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; G-83 and T-146.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-22; M-142 and T-146.
In SUB2-1; no growth at 16 and 37 degrees Celsius; when associated with G-83; M-142 and T-146.
In SUB2-201; no growth at 37 degrees Celsius; when associated with G-173 and F-403.
In SUB2-201; no growth at 37 degrees Celsius; when associated with R-122 and F-403.
In SUB2-201; no growth at 37 degrees Celsius; when associated with R-122 and G-173.
In SUB2-5; no growth at 16 degrees Celsius.
No growth at 37 degrees Celsius; when associated with DEL-135.
No growth at 37 degrees Celsius; when associated with G-8.
Decreases ATP hydrolysis. Decreases transporter activity.
Iron-sulfur clusters transporter ATM1, mitochondrial
Loss of function; significant decrease in ATP-binding; no homodimerization.
Loss of function; slight decrease in ATP-binding.
Single-strand annealing weakened protein 1
Ammonia regulation of amino acid uptake protein
37S ribosomal protein S35, mitochondrial
37S ribosomal protein S25, mitochondrial
U6 snRNA-associated Sm-like protein LSm6
54S ribosomal protein L19, mitochondrial
Decreases interaction with GCN2 and YIH1 but not with GCN20 and ribosomes.
Decreases interaction with GCN20 and polysomal association.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation; when associated with 1461-A--A-1465.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20 and strongly impairs eIF-2-alpha phosphorylation; when associated with A-1458.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 782-A-A-783 and 786-A-A-787.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 782-A-A-783 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 777-A-A-778; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-765; A-775; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-762; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-757-758-A; A-765; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not inhibit interaction with GCN20, reduces ribosome binding, ribosome binding by GCN20, weakly impairs eIF-2-alpha phosphorylation but strongly impairs eIF-2-alpha phosphorylation in a YEF3-dependent manner; when associated with A-762; A-765; A-775; 777-A-A-778; 782-A-A-783; 786-A-A-787 and A-790.
Does not interact with GCN2, impairs eIF-2-alpha phosphorylation and fails to derepress GCN4 translation in amino acid-starved cells.
SWI/SNF chromatin-remodeling complex subunit SNF5
Putative vacuolar protein sorting-associated protein TDA6
Putative uncharacterized protein YHR219W
ATP-dependent permease MDL1, mitochondrial
Decreased release of long peptides from mitochondria.
Cullin-associated NEDD8-dissociated protein 1 homolog
Lowers histone H2B deubiquitination activity.
Lowers histone H2B deubiquitination activity; when associated with A-46.
Lowers histone H2B deubiquitination activity; when associated with A-49.
2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
Abolishes diphthamide modification of elongation factor 2. Resistance to diphtheria toxin and sordarin. Sensitive to hygromycin B.
Normal sensitivity to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and leads to abnormal reduction of the DPH1 [4Fe-4S] cluster; when associated with S-362.
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and leads to abnormal reduction of the DPH1 [4Fe-4S] cluster; when associated with S-107.
Confers increased sensitivity to rapamycin and enhanced accumulation of glycogen upon TORC1 inhibition.
cAMP-dependent protein kinase regulatory subunit
Putative uncharacterized protein YCL042W
In MHR1-1; causes a defect in the partitioning of nascent mtDNA into buds and delays generation of homoplasmic cells.
Mitochondrial homologous recombination protein 1
Transcriptional regulatory protein PHO23
Eukaryotic translation initiation factor 3 subunit G
RNA polymerase I-specific transcription initiation factor RRN9
Loss of interaction with SH3 domain of PEX13. No effect on membrane association.
U3 small nucleolar RNA-associated protein 21
DNA-directed RNA polymerase III subunit RPC2
Ribonucleases P/MRP protein subunit POP6
Sphingoid long-chain base transporter RSB1
DNA-directed RNA polymerase I subunit RPA135
Lethal. Abolishes recruitment of RPA1 to Pol I. No effect; when associated with A-1104; A-1128 and A-1131.
No effect; when associated with A-1104; A-1107 and A-1128.
No effect; when associated with A-1104; A-1107 and A-1131.
No effect; when associated with A-1107; A-1128 and A-1131.
Responsible of suppression of RPA190-5 and RPA190-1 mutations.
7-keto 8-aminopelargonic acid transporter
Impairs interaction with HSL7 and prevents bud neck localization and degradation.
Probable ferric reductase transmembrane component 8
Probable 26S proteasome regulatory subunit p28
Non-structural maintenance of chromosome element 3
Putative uncharacterized protein YAR062W
54S ribosomal protein L15, mitochondrial
In wss1-pd; loss of function; when associated with A-115.
In wss1-pd; loss of function; when associated with A-119.
Impairs sorting; when associated with D-199.
Impairs sorting; when associated with D-202.
Vacuolar protein-sorting-associated protein 46
Serine/threonine-protein kinase RIM11/MSD1
General negative regulator of transcription subunit 2
Dilute domain-containing protein YPR089W
Induces dysregulation of sphingolipid synthesis; when associated with A-15, A-18, A-36 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18, and A-36.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-36 and 46-A--A-48.
Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-18, A-36 and 46-A--A-48.
Alters protein folding. Severely impaired growth. Strongly reduced growth at 35 degrees Celsius; when associated with A-1854.
Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius.
Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius. Strongly reduced growth at 35 degrees Celsius; when associated with A-1853.
Severely impaired growth. Reduced growth at 30 degrees Celsius. Strongly reduced growth at 16 degrees Celsius.
Tyrosine-protein phosphatase-like protein OCA2
Putative uncharacterized protein YML083C
Uncharacterized ABC transporter ATP-binding protein/permease YOL075C
Putative metabolite transport protein YDL199C
Impairs ATG19-binding and autophagic activity.
Impairs ATG19-binding, PE-conjugation, and autophagic activity.
Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-77.
Impairs interaction with ATG4, cleavage by ATG4, PAS localization, and autophagic activity; when associated with A-79.
No cleaving of the C-terminal R-117 by ATG4. Defect in Cvt pathway and autophagy.
Phosphatidylethanolamine amidated glycine
Significantly decreases ATG19-binding and autophagic activity.
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
ADP-ribosylation factor-binding protein GGA1
Fully functional; when associated with deletion of 1-M--K-115.
Fully functional; when associated with deletion of 260-L--T-458.
[Pyruvate dehydrogenase (acetyl-transferring)] kinase 2, mitochondrial
Phosphatidylinositol 4-phosphate 5-kinase MSS4
Probable ERAD-associated E3 ubiquitin-protein ligase ASI1
Lysophosphatidic acid:oleoyl-CoA acyltransferase 1
Respiratory supercomplex factor 2, mitochondrial
Restriction of telomere capping protein 1
Decreases phosphorylation by PKA. Leads to constitutive activation of autophagy; when associated with A-348; A-438; A-496; A-535; A-541; A-646 and A-649.
Impairs binding to ATG1; when associated with A-468.
Impairs binding to ATG1; when associated with A-469.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-541 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-541, and A-646.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-535; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-496; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-438; A-535; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-348; A-437; A-496; A-535; A-541; A-646 and A-649.
Leads to constitutive activation of autophagy; when associated with A-437; A-438; A-496; A-535; A-541; A-646 and A-649.
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1
ATP synthase subunit beta, mitochondrial
Regulator of V-ATPase in vacuolar membrane protein 1
DNA-directed RNA polymerase, mitochondrial
Carboxylic acid transporter protein homolog
Transcription initiation factor IIF subunit beta
Serine/threonine-protein kinase BCK1/SLK1/SSP31
Constitutive nuclear location and transcription activation.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with A-620 and T-629.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with T-598 and T-620.
Does not alter nuclear location and transcription activation. Constitutively cytoplasmic; when associated with T-598 and T-629.
Dominant negative transcription activator.
In diamide-induced nuclear retained form
In diamide-induced nuclear retained form; alternate
In peroxide stress-induced nuclear retained form; alternate
Interchain (with C-36 in HYR1); transient; alternate
Ribonucleoside-diphosphate reductase large chain 2
Biogenesis of lysosome-related organelles complex 1 subunit VAB2
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1
Inhibits cell growth; enhances association with pre-40S ribosomal subunits; inhibits 20S pre-rRNA to mature 18S rRNA processing; translation initiation-like defect.
Heme-responsive zinc finger transcription factor HAP1
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
Ferric reductase transmembrane component 5
N-alpha-acetyltransferase 35, NatC auxiliary subunit
Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction.
Partially active, with very weak activity toward NAD.
54S ribosomal protein MRP49, mitochondrial
Vacuolar protein sorting-associated protein 64
Cytochrome c oxidase assembly protein COX18, mitochondrial
Nuclear polyadenylated RNA-binding protein 3
Translation machinery-associated protein 64
Loss of catalytic activity and loss of binding to MAS2.
Loss of catalytic activity. No effect on the binding to MAS2.
Loss of zinc binding. Loss of catalytic activity.
Mitochondrial-processing peptidase subunit beta
Cytochrome b-c1 complex subunit 10, mitochondrial
Causes mislocalization of chitin synthase CHS3 (PubMed:16818716). Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation (PubMed:26224664).
Reduces, but does not abolish catalytic activity. Lacks autopalmitoylation.
No defect in the interaction with AFG2 or in promoting AFG2 activation.
Reduces interaction with AFG2. Loss of AFG2 activation.
Impairs the ability to arrest the cell cycle.
Inosine-5'-monophosphate dehydrogenase 3
Disrupts association with the translocase complex.
Mitochondrial import inner membrane translocase subunit TIM44
Mediator of RNA polymerase II transcription subunit 31
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; G-103 and S-219.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; G-103 and V-175.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with D-87; V-175 and S-219.
In nop1.3; thermosensitive allele showing a decrease in histone-glutamine N-methyltransferase activity upon shift to restrictive temperature; when associated with G-103; V-175 and S-219.
Chromatin modification-related protein EAF6
Negative cofactor 2 complex subunit alpha
Disrupts iron-sulfur (Fe-S) cluster assembly.
Fails to complement an isu1 deletion mutation.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-72.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-63 and S-94.
In ISU1(LVF/SSS); no growth and abolishes interaction with both JAC1 and NFS1; when associated with S-72 and S-94.
Iron sulfur cluster assembly protein 1, mitochondrial
No growth; no interaction with frataxin and SSQ1.
Wild-type growth; no interaction with SSQ1.
Lysine biosynthesis regulatory protein LYS14
Cytochrome c oxidase subunit 9, mitochondrial
Loss of proteasomal degradation in response to shift to glucose-containing growth medium.
Loss of function. Induces a decrease in transcription activation.
Structure-specific endonuclease subunit SLX1
Guanine nucleotide-binding protein subunit beta-like protein
Phosphate system positive regulatory protein PHO4
In mes1; renders the protein unstable in vitro, elevates the KM for methionine in vivo.
Putative uncharacterized helicase-like protein YBL112C
Putative uncharacterized helicase-like protein YHR218W-A
H/ACA ribonucleoprotein complex non-core subunit NAF1
Transcription initiation factor TFIID subunit 2
2-deoxyglucose-6-phosphate phosphatase 2
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Abolishes adenylyltransferase activity but not sulfurtransferase activity.
Abolishes sulfurtransferase activity but not adenylyltransferase activity.
Adenylyltransferase and sulfurtransferase UBA4
Cysteine persulfide intermediate; for sulfurtransferase activity
Glycyl thioester intermediate; for adenylyltransferase activity
Defective in spindle pole body duplication at 37 degrees Celsius.
Defective in spindle pole body duplication at 37 degrees Celsius; when associated with L-572.
Defective in spindle pole body duplication at 37 degrees Celsius; when associated with L-573.
Impairs the localization to the nuclear inner membrane.
In mps3-1; defective in spindle pole body assembly and duplication.
In nep98-7; defective in spindle organization during nuclear division and in G2/M progression.
Lethal; when associated with A-494; A-495; A-496 and A-497.
Lethal; when associated with A-494; A-495; A-496 and A-498.
Lethal; when associated with A-494; A-495; A-497 and A-498.
Lethal; when associated with A-494; A-496; A-497 and A-498.
Lethal; when associated with A-495; A-496; A-497 and A-498.
Mimics constitutive phosphorylation and blocks the regulation of the localization to nuclear inner membrane.
Spindle pole body assembly component MPS3
Putative pterin-4-alpha-carbinolamine dehydratase
ABC transporter ATP-binding protein ARB1
In RGT2-1; constitutively signaling glucose receptor.
In Rgt2(4SA); Abolishes Yck-dependent phosphorylation, interaction with MTH1 and STD1, and glucose signaling, but does not affect protein stability.
Regulator of Ty1 transposition protein 10
Mediator of RNA polymerase II transcription subunit 14
37S ribosomal protein SWS2, mitochondrial
Uncharacterized transcriptional regulatory protein YLR278C
DNA-directed RNA polymerase II subunit RPB2
25S rRNA (adenine(2142)-N(1))-methyltransferase
tRNA modification GTPase MSS1, mitochondrial
DNA replication complex GINS protein PSF3
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial
6-phosphogluconate dehydrogenase, decarboxylating 2
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI12
Y' element ATP-dependent helicase YEL077C
Trafficking protein particle complex II-specific subunit 65
Homoisocitrate dehydrogenase, mitochondrial
Ectonucleotide pyrophosphatase/phosphodiesterase 1
Abolishes agglutination; when associated with S-25.
Abolishes agglutination; when associated with S-68.
Causes a dramatic decrease in the affinity of SGF11 for SUS1.
Moderately impairs deubiquitination activity of the SAGA DUB module.
Moerately decreases the affinity of SGF11 for SUS1.
Reduces deubiquitination activity of the SAGA DUB module; when associated with A-57.
Reduces deubiquitination activity of the SAGA DUB module; when associated with A-60.
Strongly reduces deubiquitination activity of the SAGA DUB module.
Increased sensitivity toward ionizing radiation.
Causes hyperactivity with constitutive induction of PDR12.
Causes hyperactivitywith constitutive induction of PDR12.
Causes hypersensitivity to sorbate through its inability to induce PDR12.
Cytochrome c oxidase subunit 12, mitochondrial
Vacuolar protein sorting-associated protein 20
Amino-acid acetyltransferase, mitochondrial
Glycerol-3-phosphate O-acyltransferase 1
Intrastrand cross-link recognition protein
Prevents phosphorylation; when associated with A-15 and A-19.
Prevents phosphorylation; when associated with A-15 and A-235.
Prevents phosphorylation; when associated with A-19 and A-235.
Medium-chain fatty acid ethyl ester synthase/esterase 1
General negative regulator of transcription subunit 5
Non-structural maintenance of chromosome element 5
Respiratory supercomplex factor 1, mitochondrial
Probable diacetyl reductase [(R)-acetoin forming] 2
Beta-1,6-glucan synthesis-associated protein KEG1
In KEG1-1; temperature-sensitive. Increased sensitivity to chitin-binding dye Calcofluor white and zymolyase. Shows K1 killer toxin resistance, and reduced content of the cell wall beta-1,6-glucan.
Transcriptional activator of sulfur metabolism MET28
Loss of function in calcium homeostasis.
Uncharacterized mitochondrial membrane protein FMP10
U3 small nucleolar RNA-associated protein 4
Confers sensitivity to HU indicating a disturbed activity in DNA replication.
Confers sensitivity to HU indicating a disturbed activity in DNA replication; confers a SPT- phenotype indicating a disturbed activity in transcription.
In pob3-11; causes severe defects in rate of growth; when associated with C-109.
In pob3-11; causes severe defects in rate of growth; when associated with H-20.
In pob3-1; causes severe defects in rate of growth; when associated with K-419 and T-489.
In pob3-1; causes severe defects in rate of growth; when associated with R-78 and K-419.
In pob3-1; causes severe defects in rate of growth; when associated with R-78 and T-489.
In pob3-21; causes severe defects in rate of growth.
No change of sensitivity to HU; confers a SPT- phenotype indicating a disturbed activity in transcription.
NPC intracellular sterol transporter 1-related protein 1
Sphingolipids mislocalization and no growth at 38 degrees Celsius.
Glycerol-3-phosphate dehydrogenase [NAD(+)] 2, mitochondrial
37S ribosomal protein S16, mitochondrial
Eukaryotic translation initiation factor eIF-1
Vacuolar protein sorting-associated protein 53
Trafficking protein particle complex subunit BET3
Ankyrin repeat-containing protein YIL001W
Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
Completely inactive as thiol transferase. No activity recovered against 1-chloro-2,4-dinitrobenzene (CDNB).
No effect on thiol transferase activity.
No effect on thiol transferase activity. No effect on thiol transferase activity; when associated with D-173.
H/ACA ribonucleoprotein complex subunit NOP10
Abolishes palmitoyltransferase activity and interaction with SHR5.
Abolishes palmitoyltransferase activity.
In strain: CLIB 556 haplotype Ha2 and CLIB 630 haplotype Ha2.
In strain: CLIB 95, CLIB 382, CLIB 388, CLIB 556 haplotype Ha2, CLIB 630, K1, R12, R13, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha1.
N-(5'-phosphoribosyl)anthranilate isomerase
Meiosis-specific serine/threonine-protein kinase MEK1
N-alpha-acetyltransferase 38, NatC auxiliary subunit
Pyridoxamine 5'-phosphate oxidase YLR456W homolog
Plasma membrane-associated coenzyme Q6 reductase PGA3
Cu(2+) suppressing and bleomycin sensitive protein 1
Serine/threonine-protein phosphatase 4 regulatory subunit 3
Cold-sensitive. Abolishes interaction with BRF1/TDS4.
Cold-sensitive. No effect on interaction with BRF1/TDS4.
DNA-directed RNA polymerase III subunit RPC6
Temperature-sensitive; cold-sensitive. Abolishes interaction with BRF1/TDS4. Stabilizes Pol III open complex formation.
Pentamidine resistance factor, mitochondrial
Slightly reduced interaction with KTI11.
Putative uncharacterized protein YCL002C
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2
Abolishes coenzyme Q biosynthesis. Able to autophosphorylate in cis.
Abolishes coenzyme Q biosynthesis. Enhanced autophosphorylation in cis.
In coq8-2; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-3; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8. Impairs coenzyme Q6 biosynthesis.
In coq8-4; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-5; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8. Impairs coenzyme Q6 biosynthesis.
In coq8-6; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
In coq8-8; impairs growth on ethanol and/or glycerol as a carbon source, but retains steady-state levels of COQ8.
2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-239.
Resistance to diphtheria toxin. Abolishes [4Fe-4S] cluster binding and catalytic activity; when associated with S-368.
Uncharacterized endoplasmic reticulum membrane protein YJR015W
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Vacuolar protein sorting-associated protein 35
Probable phospholipid-transporting ATPase NEO1
In TOR2-1; confers resistance to rapamycin.
Leads to growth defect at 37 degrees Celsius, probably by disrupting the intramolecular interaction of the N-termini with the middle domains; when associated with A-453.
Leads to growth defect at 37 degrees Celsius, probably by disrupting the intramolecular interaction of the N-termini with the middle domains; when associated with A-493.
25S rRNA (uridine(2843)-N(3))-methyltransferase
Prevents insertion into the outer membrane and increases the efficiency of import into the intermembrane space.
Dolichyl-phosphate-mannose--protein mannosyltransferase 6
Adenylate kinase isoenzyme 6 homolog FAP7
Decreases the processing of 20S rRNA; when associated with A-82.
Decreases the processing of 20S rRNA; when associated with A-84.
Maltose fermentation regulatory protein MAL33
Impairs oligomerization. Reduces ATPase activity by 92%.
ATP synthase subunit delta, mitochondrial
Probable metabolite transport protein YDR387C
In npl4-1; nuclear-targeted proteins accumulate in the cytoplasm.
Uncharacterized membrane protein YPR027C
Nuclear polyadenylated RNA-binding protein 4
Alkylphosphocholine resistance protein LEM3
12000-fold decrease in catalytic activity and 50000-fold decrease in catalytic efficiency, with ferricyanide as electron acceptor.
54S ribosomal protein L49, mitochondrial
Leads to a decrease of the catalytic activity and the ubiquitination efficiency.
Prevents the stabilization of the enzyme by oxaloacetate and impairs the catalytic activity.
Putative uncharacterized protein YAR069C
Stabilizes ubiquitin pathway substrates; when associated wirh Ala-109.
Stabilizes ubiquitin pathway substrates; when associated wirh Ala-111.
Ubiquitin carboxyl-terminal hydrolase RPN11
Mitochondrial distribution and morphology protein 30
DSC E3 ubiquitin ligase complex subunit 3
Abnormal TORC1 activation in glutamine-replete conditions. Sensitive to caffeine. Resistance to tunicamycin (endoplasmic reticulum stressor) administered together with FK506 (calcineurin inhibitor).
Abolishes interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Decreases interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Decreases interaction with and activation of TOR1.
Decreases localization to the vacuolar membrane and results in rapamycin sensitivity; when associated with A-475.
Decreases localization to the vacuolar membrane and results in rapamycin sensitivity; when associated with S-522.
Increases activity in presence of glutamine.
Mildly decreases interaction with and activation of TOR1 in response to glutamine. Sensitive to rapamycin.
Phosphatidylinositol transfer protein SFH5
In TEF5-7; reduces translation efficiency and enhances translation fidelity.
Loss of catalytic activity, but still binds to eEF1A.
Reduces translation efficiency and enhances translation fidelity.
Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
Reduces the transferase reaction 12-fold.
Reduces the transferase reaction 5-fold.
Dehydrodolichyl diphosphate synthase complex subunit SRT1
Polyphosphatidylinositol phosphatase INP52
Translationally-controlled tumor protein homolog
Cardiolipin-specific deacylase 1, mitochondrial
Cell wall integrity and stress response component 3
RNA polymerase II holoenzyme cyclin-like subunit
Conserved oligomeric Golgi complex subunit 1
Causes progressive telomere shortening and cell senescence, but does not impair interaction with the telomerase RNP.
Causes progressive telomere shortening and cell senescence.
Causes telomere shortening, but does not impair interaction with the telomerase RNP.
Impairs interaction with the telomerase RNP and causes progressive telomere shortening and cell senescence.
Impairs interaction with the telomerase RNP and causes telomere shortening.
Reduces ability to dimerize and causes telomere shortening.
Reduces binding to DNA and RNA, and causes progressive telomere shortening and cell senescence.
Fails to reduce intracellular dinucleoside polyphosphate levels.
U3 small nucleolar RNA-associated protein 13
Only moderately reduces the thioesterase activity.
Impaired tail-anchored protein insertion and impaired GET3 localization and loss of the GET1-GET2 heterotetramer; when associated with A-150.
Impaired tail-anchored protein insertion, impaired GET3 localization and loss of the GET1-GET2 heterotetramer; when associated with A-157.
Structural maintenance of chromosomes protein 5
Reduces kcat 100-fold. Reduces substrate affinity 900-fold.
Reduces kcat over 100000-fold. Reduces substrate affinity 11-fold.
Vacuolar cation-transporting ATPase YPK9
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial
Cytochrome c oxidase subunit 6, mitochondrial
Transcriptional regulatory protein SAP30
Leads to sensitivity to sertraline, when associated with A-273.
Leads to sensitivity to sertraline, when associated with S-275.
Complete loss of covalent binding to ATG12.
No effect on conjugating activity. Normal autophagic activity.
Strong decrease of binding to ATG12. No more formation of ATG12-ATG5 conjugate. Defect in autophagy.
Protein phosphatase type 2A regulatory subunit RTS3
U4/U6 small nuclear ribonucleoprotein PRP3
Impairs interaction with ubiquitin; when associated with A-109.
Impairs interaction with ubiquitin; when associated with A-110.
Impairs interaction with ubiquitin; when associated with A-135.
Impairs interaction with ubiquitin; when associated with A-136.
Putative uncharacterized protein YDR034C-A
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Blocks autocatalytic processing of the precursor protein.
Proton donor; shared with dimeric partner
Mediator of RNA polymerase II transcription subunit 16
Serine/threonine-protein phosphatase 2B catalytic subunit A1
54S ribosomal protein L27, mitochondrial
Mitochondrial tRNA-specific 2-thiouridylase 1
PAN2-PAN3 deadenylation complex catalytic subunit PAN2
Cytochrome c oxidase subunit 7, mitochondrial
t-SNARE affecting a late Golgi compartment protein 2
Putative uncharacterized protein YER076C
Transcription initiation factor TFIID subunit 11
Mitochondrial import inner membrane translocase subunit TIM54
DNA-directed RNA polymerase I subunit RPA190
Ectonucleotide pyrophosphatase/phosphodiesterase 2
In PBS2-13; loss of SH3-domain interaction.
Topoisomerase I damage affected protein 7
Abolishes the interaction with FKH1; when associated with A-776.
Abolishes the interaction with FKH1; when associated with A-785.
Reduces the interaction with FKH1; when associated with A-776.
Reduces the interaction with FKH1; when associated with A-785.
Peptide chain release factor 1, mitochondrial
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Serine/threonine-protein phosphatase 2A activator 2
Suppressor of hydroxyurea sensitivity protein 2
Ribosome quality control complex subunit 1
Guanine nucleotide exchange factor subunit RIC1
In PTR3-17; constitutively active, confers increased STP1 processing in the absence of amino acids.
In PTR3-5; constitutively active and hyperphosphorylated, confers increased STP1 processing in the absence of amino acids.
Loss of function. Abolishes hyperphosphorylation of PTR3 and, consequently, results in a failure to activate STP1.
Oligosaccharide translocation protein RFT1
Mitochondrial ATPase complex subunit ATP10
Measured from a recombinant protein expressed in E.coli.
Abolishes ARP2/3 complex activation, but not actin binding.
In ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-21.
In ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-24.
In ABP1-2; strongly reduces actin binding and abolishes ARP2/3 complex activation.
In ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-94.
In ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-96.
In ABP1-4; no effect; when associated with A-122.
In ABP1-4; no effect; when associated with A-125.
In ABP1-5; moderately reduces actin binding and abolishes ARP2/3 complex activation.
Dominant negative mutant; cells show a severe synthetic sick phenotype on glycerol/lactate medium.
Loss of function. Abolished homodimerization.
Putative uncharacterized protein YOL114C
Chromosome segregation in meiosis protein 4
Nuclear cap-binding protein complex subunit 1
Reduces 18S rRNA acetylation by 80% and tRNA acetylation by 47%.
Total loss of 18S rRNA acetylation and tRNA acetylation.
Temperature-sensitive. Reduced sporulation efficiency.
Temperature-sensitive; when associated with L-806. Altered cell cycle distribution.
Temperature-sensitive; when associated with M-881. Altered cell cycle distribution.
Blocks Ca(2+)-stimulated auto-processing and its catalytic activity towards substrates.
No effect on auto-processing and catalytic activity towards substrates.
Prevents auto-cleavage at Arg-72 but not at Lys-86. Does not affect catalytic activity; when associated with A-86.
Prevents auto-cleavage at Lys-86 but not at Arg-72. Does not affect catalytic activity; when associated with A-72.
In 1014; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; partially K1 killer toxin-sensitive; when associated with DEL-GSC2.
In 1082; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with DEL-GSC2.
In 1093; temperature-sensitive mutant; higher beta-glucan content of cells; when associated with I-605 and DEL-GSC2.
In 1093; temperature-sensitive mutant; higher beta-glucan content of cells; when associated with T-761 and DEL-GSC2.
In 1104; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with E-920 and DEL-GSC2.
In 1104; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with V-823 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with G-932; D-934; Y-1020; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; D-934; Y-1020; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; D-934; N-1047 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; D-934; Y-1020 and DEL-GSC2.
In 1114; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with T-853; G-932; Y-1020; N-1047 and DEL-GSC2.
In 1125; temperature-sensitive mutant; lower beta-glucan content of cells and partial K1 killer toxin-resistant phenotype; when associated with Y-1258 and DEL-GSC2.
In 1125; temperature-sensitive mutant; lower beta-glucan content of cells and partially K1 killer toxin-resistant; when associated with D-1520 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with N-329; N-335 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-146; N-329 and DEL-GSC2.
In 1132; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-146; N-335 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with F-872; K-907 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with F-872; S-982 and DEL-GSC2.
In 1144; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with K-907; S-982 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with N-877; P-977 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with N-877; S-899 and DEL-GSC2.
In 1154; temperature-sensitive mutant which is able to grow at 25 degrees Celsius but fails to grow at temperatures above 35 degrees Celsius; defective in 1,3-beta-glucan synthesis and thus has lower beta-glucan content; hypersensitive to echinocandin B and to a chitin-binding reagent, Calcofluor white; fails to grow in a low glucose medium; when associated with S-899; P-977 and DEL-GSC2.
In 1163; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with L-713 and DEL-GSC2.
In 1163; temperature-sensitive mutant; no gross alteration in beta-glucan content of cells; when associated with V-722 and DEL-GSC2.
In A6; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with DEL-GSC2.
In ACR1A3; selectively resistant to antibiotic arborcandin C.
In ACR79-5; selectively resistant to antibiotic arborcandin C.
In F4; temperature-sensitive mutant; lower beta-glucan content of cells; when associated with DEL-GSC2.
Uncharacterized membrane protein YCR061W
Altered inheritance of mitochondria protein 3
Completely abolishes interaction with PCF11. No effect on growth; when associated with A-136.
Completely abolishes interaction with PCF11. No effect on growth; when associated with A-137.
Compromises interaction with PCF11. No effect on growth.
mRNA cleavage and polyadenylation factor CLP1
Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation.
Displays a 60-fold increase in spontaneous mutation accumulation.
Displays an increase in spontaneous mutation accumulation.
In strain: SK1 and YJM320; forms a non-functional heterodimer with MHL1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains.
In strain: SK1, YJM320, YJM339 and YJM421.
Reduces ATPase activity by 62%. Displays 60-fold increase in spontaneous mutation accumulation.
Reduces ATPase activity by 84%. Displays 11000-fold increase in spontaneous mutation accumulation.
Altered inheritance of mitochondria protein 19, mitochondrial
Uncharacterized ATP-dependent helicase IRC20
Regulator of Ty1 transposition protein 102
Chromatin-remodeling complexes subunit NGG1
In pol3-13; synthetically lethal with mutations of NBP35, DRE2 and TAH18.
Uncharacterized vacuolar protein YLR297W
Mitochondrial import receptor subunit TOM5
Reverses the aerobic defects of yeast strains lacking superoxide dismutase (SOD). Associated with elevated copper ion accumulation and increased sensitivity to the toxic effects of copper and cadmium. Exhibits no additional sensitivity to manganese.
Fails to release from the spliceosome; when associated with D-349.
Fails to release from the spliceosome; when associated with H-548.
Has small amount of ATPase activity, but no splicing activity.
In PRP2-dn1; 40% of wild-type RNA-stimulated ATPase activity; splicing activity abolished; accumulates stalled splicing complexes.
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
Spliceosome binding mutant; not active in splicing; when associated with N-551, or D-349 and H-548. Loss of interaction with SPP2.
Spliceosome binding mutant; not active in splicing; when associated with N-551.
Spliceosome binding mutant; not active in splicing; when associated with N-551. Almost wild-type RNA-dependent ATPase activity.
Temperature-sensitive; decreased interaction with SPP2, decreased cell growth on benomyl and decreased splicing at elevated temperatures; when associated with D-349 and H-548.
Wild-type RNA-dependent ATPase activity; bound tightly to the spliceosome and after addition of ATP released from the spliceosome.
Cytochrome b-c1 complex subunit 9, mitochondrial
Putative uncharacterized oxidoreductase YDR541C
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Abolishes phosphoinositide binding and Golgi localization; when associated with A-178.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-181.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-88.
Abolishes phosphoinositide binding and Golgi localization; when associated with A-97.
Alters phosphorylation but has not effect on Golgi enzymes localization.
Vacuolar protein sorting-associated protein 74
U4/U6 small nuclear ribonucleoprotein PRP4
Abolishes binding to COPI coatomer complex.
Multicopy suppressor of SEC21 protein 27
Inner centromere protein-related protein SLI15
Failure to mediate ceramide transport; when associated with A-340.
Failure to mediate ceramide transport; when associated with A-472.
Mitochondrial import inner membrane translocase subunit TIM50
Anaphase-promoting complex subunit CDC23
In CDC23-1; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-2; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-37; G2/M cell cycle arrest at 30 degrees Celsius.
In CDC23-39; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-40; G2/M cell cycle arrest at 37 degrees Celsius; when associated with R-103.
In CDC23-40; G2/M cell cycle arrest at 37 degrees Celsius; when associated with V-573.
In CDC23-41; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-44; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-47; G2/M cell cycle arrest at 37 degrees Celsius; when associated with G-213.
In CDC23-47; G2/M cell cycle arrest at 37 degrees Celsius; when associated with W-583.
In CDC23-49; G2/M cell cycle arrest at 37 degrees Celsius; when associated with E-306.
In CDC23-49; G2/M cell cycle arrest at 37 degrees Celsius; when associated with P-326.
In CDC23-4; G2/M cell cycle arrest at 36 degrees Celsius.
In CDC23-50; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-51; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-52; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-53; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-54; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-56; G2/M cell cycle arrest at 37 degrees Celsius.
In CDC23-6; G2/M cell cycle arrest at 36 degrees Celsius.
Strongly reduces formation of the thioester intermediate with ubiquitin.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with K-1014.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with K-1016.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with P-912.
Strongly reduces formation of the thioester intermediate with ubiquitin; when associated with P-914.
No effect on activity. Reduces affinity for ornithine 200-fold.
Reduces activity by 33%. Reduces affinity for ornithine 30-fold.
Reduces activity by 50%. Reduces affinity for ornithine 20-fold.
Reduces activity by 70%. Reduces affinity for ornithine 18-fold.
Reduces activity by 90%. Reduces affinity for ornithine 6-fold.
Reduces activity by 95%. Reduces affinity for ornithine 2-fold.
U3 small nucleolar RNA-associated protein 15
Loss of activity; important for dimerization.
Histone-lysine N-methyltransferase, H3 lysine-4 specific
LETM1 domain-containing protein YLH47, mitochondrial
U3 small nucleolar RNA-associated protein 6
54S ribosomal protein L33, mitochondrial
RNA polymerase II transcriptional coactivator SUB1
Mitotic spindle checkpoint component MAD2
Completely abolishes the enzymatic activity.
Phosphatidate cytidylyltransferase, mitochondrial
Mediator of RNA polymerase II transcription subunit 2
Reduces expression of several genes from the endogenous 2-micron plasmid and augments expression of numerous iron-response genes.
Intron-encoded DNA endonuclease aI5 beta
Interaction with RRP46 abolished; when associated with F-162.
Interaction with RRP46 abolished; when associated with T-246.
Interaction with RRP46 abolished; when associated with T-274 and Y-276.
Interaction with RRP46 abolished; when associated with Y-230 and T-274.
Interaction with RRP46 abolished; when associated with Y-230 and Y-276.
Vacuolar protein sorting-associated protein 5
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
In PRP28-101; no growth at 15 and 37 degrees Celsius.
In PRP28-102; no growth at 25 degrees Celsius or lower.
In PRP28-103; no growth at 15 and 37 degrees Celsius.
In PRP28-117; no growth at 15 degrees Celsius.
In PRP28-1; no growth at 15 degrees Celsius.
In PRP28-32; no growth at 15 degrees Celsius.
In PRP28-36; slow growth at 30 degrees Celsius, and no growth at 15 and 37 degrees Celsius.
In PRP28-37; no growth at 37 degrees Celsius.
In PRP28-52; no growth at 37 degrees Celsius.
In PRP28-55; no growth at 37 degrees Celsius.
In PRP28-56; no growth at 37 degrees Celsius; when associated with F-440 and E-584.
In PRP28-56; no growth at 37 degrees Celsius; when associated with F-440 and S-546.
In PRP28-56; no growth at 37 degrees Celsius; when associated with S-546 and E-584.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-438 and D-486.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-438 and L-468.
In PRP28-61; no growth at 37 degrees Celsius; when associated with L-468 and D-486.
In PRP28-66; no growth at 37 degrees Celsius; when associated with A-541; V-549; N-580 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; N-580 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; V-549 and N-580.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; A-541; V-549 and V-586.
In PRP28-66; no growth at 37 degrees Celsius; when associated with T-449; V-549; N-580 and V-586.
In PRP28-99; no growth at 15 and 37 degrees Celsius.
Pre-mRNA-splicing ATP-dependent RNA helicase PRP28
54S ribosomal protein L17, mitochondrial
Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and L-1773 or D-1769; L-1773; S-1879 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and S-1771 or D-1769; S-1771; S-1879 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and E-1881.
Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and S-1879.
Does not affect oligomerization; when associated with S-1771 and L-1773 or S-1771; L-1773; S-1879 and E-1881.
Loss of transferase activity; when associated with S-1772.
Loss of transferase activity; when associated with S-1774.
Superficial pseudohyphal growth protein 1
Mitochondrial thiamine pyrophosphate carrier 1
Abolishes S-adenosyl-L-methionine binding and methyltransferase activity.
Abolishes methyltransferase activity, but not S-adenosyl-L-methionine binding.
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Abolishes self-association; when associated with G-332.
Abolishes self-association; when associated with L-335.
In mcm10-1; diminishes interaction with MCM7.
In mcm10-43; abolishes self-association and diminishes interaction with MCM7.
Inhibits interaction with POL30/PCNA and abolishes cell proliferation.
Temperature sensitive; loss of CDC17 stabilization.
In strain: CLIB 219, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha2.
In strain: CLIB 388, CLIB 410, CLIB 556 haplotype Ha2, CLIB 630, YIIc12 haplotype Ha2 and YIIc17 haplotype Ha2.
In strain: CLIB 95, CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13, YIIc12 and YIIc17.
In strain: CLIB 95, CLIB 382, CLIB 556 haplotype Ha1, K1, R12, R13, YIIc12 haplotype Ha1 and YIIc17 haplotype Ha1.
In strain: CLIB 95, CLIB 382, K1, R12, R13, YIIc12 haplotype Ha1 and YIIc17 haplotype Ha1.
DNA damage checkpoint control protein RAD17
Altered inheritance of mitochondria protein 36, mitochondrial
Y' element ATP-dependent helicase YML133C
10-fold decrease in interaction with ISU1.
Iron-sulfur cluster biogenesis chaperone, mitochondrial
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Chromatin modification-related protein EAF1
Dihydroxy-acid dehydratase, mitochondrial
General negative regulator of transcription subunit 4
Cysteine-rich and transmembrane domain-containing protein YDR210W
Probable vacuolar amino acid transporter YPQ1
Abolishes ubiquitin-binding and vacuole sorting of ubiquitinated proteins.
Vacuolar protein-sorting-associated protein 36
Poisonous alpha-tubulins that cause lethality. Microtubules do not depolymerize.
In strain: YJM 269, YJM 270, YJM 326 and YJM 1129.
Abolishes rapamycin-resistance of mutants E-1972; I-1972 and R-1972.
Confers resistance to rapamycin. Abolishes interaction with FKBP-rapamycin.
In DRR1-1; confers resistance to rapamycin. Abolishes interaction with FKBP-rapamycin.
In DRR1-27; confers resistance to rapamycin.
Sensitive to high hydrostatic pressure (mechanical stress).
Causes hypersensitivity to DNA-damage-inducing agents and impairs sporulation.
No effect. No effect; when associated with R-124 and R-127.
No effect; when associated with R-120; R-121 and R-124.
No effect; when associated with R-120; R-121 and R-127.
Sorting assembly machinery 50 kDa subunit
Probable serine/threonine-protein kinase KKQ8
Disrupts iron-sulfur (Fe-S) cluster assembly and decreases cytosolic tRNA thiolation. Exhibits constitutive up-regulation of the genes of the cellular iron uptake system.
Suppressor of HU sensitivity involved in recombination protein 1
6-phosphogluconolactonase-like protein 1
In RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.
Putative uncharacterized protein YPL278C
In SRB4-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation.
Mediator of RNA polymerase II transcription subunit 17
Membrane-anchored lipid-binding protein LAM4
Small nuclear ribonucleoprotein-associated protein B
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Putative uncharacterized protein YPR203W
L-threo-3-hydroxyaspartate ammonia-lyase
Constitutively in the GDP-bound conformation. Causes loss of function during vacuolar morphogenesis.
Constitutively in the GTP-bound conformation. Complements the fragmented vacuolar morphology of ypt7 null mutant cells.
DNA topoisomerase 2-associated protein PAT1
Vacuolar membrane-associated protein IML1
Putative aryl-alcohol dehydrogenase AAD16
Pore and endoplasmic reticulum protein of 33 kDa
ARF guanine-nucleotide exchange factor 2
Increased microtubule polymerization and depolymerization rates. Increased microtubule stability. Decreased kinesin KIP3 subcellular location at microtubule plus ends.
DNA-directed RNA polymerase II subunit RPB7
Lowers nucleic-acid binding of RPB4-RPB7 by 10-fold; no effect on association with Pol II core complex; abolishes transcriptional activity of Pol II.
No effect on nucleic-acid binding of RPB4-RPB7 and on association with Pol II core complex; abolishes transcriptional activity of Pol II.
Eukaryotic translation initiation factor 4E
Activates HOG1 in a constitutive manner, without the need of a stimulating stress.
Impairs catalytic activity and nuclear translocation.
Impairs catalytic activity, expression of CTT1 and increases sensitivity to osmotic shock.
Impairs catalytic activity, nuclear translocation, expression of CTT1 and increases sensitivity to osmotic shock.
Impairs expression of CTT1 and increases sensitivity to osmotic shock.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1
Altered inheritance of mitochondria protein 18, mitochondrial
No effect on growth rate. Restores cell growth; when associated with Q-315.
Probable loss of ATP binding. Complete loss of catalytic activity.
Restores cell growth; when associated with Q-315.
Severe loss of catalytic activity and cooperativity between the 2 ATP-binding regions. Lethal. Restores cell growth; when associated with A-358; A-369; S-471; A-471 or H-475.
Severe loss of catalytic activity without affecting cooperativity between the 2 ATP-binding regions. Slight reduction in growth rate.
Severe loss of catalytic activity. Lethal.
Slight reduction in growth rate. Restores cell growth; when associated with Q-315.
Transcriptional activator/repressor MOT3
Abolishes binding to phosphatidylinositol 4-phosphate.
Abolishes both cholesterol binding and biological function.
Reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
Slight reduction in cholesterol transport.
Slight reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
Strong reduction in cholesterol binding without affecting phosphatidylinositol 4-phosphate binding.
Strong reduction in cholesterol transport.
Strong reduction in cholesterol transport. Abolishes binding to phosphatidylinositol 4-phosphate.
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8
Uncharacterized membrane protein YNL019C
Putative uncharacterized protein YCL068C
Almost completely abolishes catalytic activity.
Completely abolishes catalytic activity; when associated with V-387.
Completely abolishes catalytic activity; when associated with V-389.
Protein arginine N-methyltransferase HSL7
Cytochrome c oxidase subunit 4, mitochondrial
In allele LHS1-1; constitutive ATPase activity.
Impairs the formation of protofilaments; when associated with K-90. Also impairs the formation of protofilaments; when associated with E-94. Also impairs the formation of protofilaments; when associated with E-107. Also impairs the formation of protofilaments; when associated with E-114.
Leads to severe sorting defects. Impairs the formation of protofilaments; when associated with E-52.
Leads to severe sorting defects; when associated with E-25 and E-29.
Leads to severe sorting defects; when associated with E-25 and E-36.
Leads to severe sorting defects; when associated with E-29 and E-36.
Leads to severe sorting defects; when associated with K-102 and K-109.
Leads to severe sorting defects; when associated with K-95 and K-102.
Leads to severe sorting defects; when associated with K-95 and K-109.
Probable electron transfer flavoprotein subunit beta
54S ribosomal protein L10, mitochondrial
Ubiquitin carboxyl-terminal hydrolase MIY1
GPI ethanolamine phosphate transferase 2
In GPI7-2; temperature-sensitive mutant that shows defects in cell separation and a daughter cell-specific growth defect.
No growth at 13 degrees Celsius; when associated with D-305.
No growth at 13 degrees Celsius; when associated with G-637.
No growth at 13 degrees Celsius; when associated with P-564.
No growth at 13 degrees Celsius; when associated with Q-431 and G-472.
No growth at 13 degrees Celsius; when associated with T-191 and G-472.
No growth at 13 degrees Celsius; when associated with T-191 and Q-431.
No growth at 13 degrees Celsius; when associated with V-260.
No growth at 13 degrees Celsius; when associated with V-291.
No growth at 13 degrees Celsius; when associated with V-306.
Impairs interaction with MDN1. Blocks progression of the nascent pre-60S subunit and subsequent export to the cytoplasm.
Severe growth defect; lowers SRP-dependent and SRP-independent translocation.
Severe growth defect; lowers SRP-dependent translocation.
Hydroxyacylglutathione hydrolase, cytoplasmic isozyme
Glycerol uptake/efflux facilitator protein
Probable pyridoxal 5'-phosphate synthase subunit SNZ2
Sterol regulatory element-binding protein ECM22
Coupling of ubiquitin conjugation to ER degradation protein 1
Mitochondrial import inner membrane translocase subunit TIM12
4-hydroxybenzoate polyprenyltransferase, mitochondrial
Required for respiratory growth protein 7, mitochondrial
Signal recognition particle subunit SRP14
In aah1-2; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-3; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-4; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-6; impairs AAH1 degradation during postdiauxic growth and leads to weak interaction with SAF1.
In aah1-7; impairs AAH1 degradation during postdiauxic growth.
RNA polymerase I-specific transcription initiation factor RRN3
Required for respiratory growth protein 8, mitochondrial
Eukaryotic translation initiation factor 3 subunit C
General transcription and DNA repair factor IIH subunit TFB4
Single-stranded nucleic acid-binding protein
Uncharacterized trans-sulfuration enzyme YHR112C
Myosin tail region-interacting protein MTI1
Abolishes interaction with VSP4 and dimerization.
Abolishes interaction with VSP4, no effect on dimerization.
Abolishes interaction with VSP60 and DID2.
Vacuolar protein sorting-associated protein VTA1
Double-strand break repair protein MRE11
Glyceraldehyde-3-phosphate dehydrogenase 2
Abolishes interaction with BUB1 and BUB3 and spindle checkpoint function.
Spindle assembly checkpoint component MAD1
Induces an increase of mitochondrial fission.
Transcriptional activator/repressor GIS1
In strain: MMW1-15h2, MMW1-2h2, Sgu52E and Sgu52F.
Sigma-like sequence protein 1, mitochondrial
Putative aryl-alcohol dehydrogenase AAD15
Vacuolar protein sorting-associated protein 30
Small glutamine-rich tetratricopeptide repeat-containing protein 2
Delays the appearance and decreases the level of 25S rRNA.
External NADH-ubiquinone oxidoreductase 2, mitochondrial
Conserved oligomeric Golgi complex subunit 2
Glyceraldehyde-3-phosphate dehydrogenase 3
Translation initiation factor eIF-2B subunit alpha
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
In TRF4-194; lethal; when associated with A-195; A-196 and A-198.
In TRF4-282; lethal; when associated with A-282.
In TRF4-282; lethal; when associated with A-285.
In TRF4-378; lethal; when associated with A-378.
In TRF4-378; lethal; when associated with A-381.
In TRF4-425; lethal; when associated with A-425.
In TRF4-425; lethal; when associated with A-428 and A-429.
In TRF4-502; lethal; when associated with A-502.
In TRF4-502; lethal; when associated with A-504.
In TRF4-559; slow growth; when associated with A-559.
Centromere DNA-binding protein complex CBF3 subunit C
Putative uncharacterized protein YER138W-A
Histone H3-like centromeric protein CSE4
In CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with S-176.
In CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with T-218.
In CSE4-110; impairs nuclear division by disrupting the core centromere structure.
In CSE4-111; impairs nuclear division by disrupting the core centromere structure.
Trafficking protein particle complex subunit BET5
Protein phosphatase 1 regulatory subunit SDS22
Abolishes transcriptional activation of target genes.
Reduces transcriptional activation of target genes.
pH-response transcription factor pacC/RIM101
Putative ISWI chromatin-remodeling complex subunit YPL216W
Mitochondrial distribution and morphology protein 38
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Significantly low viability and relatively normal Ca(2+) accumulation.
Substitution by hydrophilic amino acids causes lethality and low Ca(2+) accumulation.
Substitution by hydrophobic, large amino acids does not cause lethality nor low Ca(2+) accumulation.
Altered inheritance of mitochondria protein 41, mitochondrial
Conserved oligomeric Golgi complex subunit 5
Mitochondrial inner membrane i-AAA protease supercomplex subunit MGR1
Decreases catalytic activity 2-fold, but retains substrate binding properties.
Geranylgeranyl transferase type-1 subunit beta
In cal1-1; completely abolishes geranylgeranyl transferase activity. Displays a defect in nuclear division and bud formation.
In cdc43-2; severely impairs the geranylgeranyl transferase activity.
In cdc43-3; severely impairs the geranylgeranyl transferase activity.
In cdc43-4,5,6; severely impairs the geranylgeranyl transferase activity.
In cdc43-7; severely impairs the geranylgeranyl transferase activity.
Increases KM for isoprenoid substrate 29-fold.
Increases KM for peptide substrate 12-fold.
2-deoxyglucose-6-phosphate phosphatase 1
Aspartate aminotransferase, mitochondrial
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit
Methylated-DNA--protein-cysteine methyltransferase
N-terminal acetyltransferase B complex subunit MDM20
ATP-dependent 6-phosphofructokinase subunit alpha
Abolishes sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'D-718' in subunit beta.
Increases the KM for fructose 6-phosphate 20 fold.
Reduces maximal activity of the holoenzyme by 50%. Completely abolishes catalytic activity; when associated with 'S-348' in subunit beta.
Reduces sensitivity of the holoenzyme to fructose 2,6-bisphosphate activation; when associated with 'S-853' in subunit beta.
In GLC5-1; low glycogen accumulation and sporulation deficiency.
Peptidyl-prolyl cis-trans isomerase CYP8
ATP-dependent (S)-NAD(P)H-hydrate dehydratase
Translation machinery-associated protein 7
Restriction of telomere capping protein 3
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP51
In FAM1-1; extragenic suppressor mutation, which creates a new initiation codon and adds 18 amino acids to the N-terminus of the protein. This extension has all the characteristics of a mitochondrial targeting sequence and the FAM1-1 mutant protein is indeed imported into the mitochondria, where it restores respiratory growth to a deletion of the mitochondrial beta-keto-acyl synthase CEM1.
Eliminates glycogen accumulation; when associated with F-230 and F-232.
Eliminates glycogen accumulation; when associated with F-230 and F-367.
Eliminates glycogen accumulation; when associated with F-232 and F-367.
54S ribosomal protein L12, mitochondrial
Impairs interaction with UBC2, but does not affect degradation of N-end rule substrates.
No effect on interaction with UBC2, but inhibits degradation of N-end rule substrates.
Loss of acireductone dioxygenase activity.
No loss of acireductone dioxygenase activity.
Putative transferase CAF17, mitochondrial
ERAD-associated E3 ubiquitin-protein ligase component HRD3
DNA-directed RNA polymerase III subunit RPC7
Maintenance of telomere capping protein 2
Ferric/cupric reductase transmembrane component 1
Valine/tyrosine/tryptophan amino-acid permease 1
Phosphate system positive regulatory protein PHO81
In PRP43-DN1; dominant-negative phenotype.
In PRP43-DN2; dominant-negative phenotype.
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43
Putative cyclin-dependent kinase inhibitor SPL2
Mitochondrial nicotinamide adenine dinucleotide transporter 2
Endoplasmic reticulum membrane protein complex subunit 10
Abolishes proteolytic processing of ATP6, but still promotes assembly of the ATP6 precursor into the ATPase CF(0) particle.
Mitochondrial inner membrane protease ATP23
In SRB5-1; suppresses the phenotypic defects of an RNA polymerase II CTD truncation.
Mediator of RNA polymerase II transcription subunit 18
Cytochrome b translational activator protein CBS1, mitochondrial
In CBS1-1, which is respiratory deficient.
Loss of function, causes respiratory deficiency.
Eukaryotic translation initiation factor 5B
Impairs the GTPase activity, but not the ribosome joining function.
Reduces GTP binding and impairs subunit joining and ribosome-dependent GTP hydrolysis.
Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing]
Phosphoribosylaminoimidazole carboxylase
Deoxyuridine 5'-triphosphate nucleotidohydrolase
6-phosphogluconate dehydrogenase, decarboxylating 1
Bypasses the requirement for phosphatase YVH1 for the release of MRT4.
In DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with K-67.
In DUO1-1; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with V-157.
In DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with I-124.
In DUO1-2; produces abnormal spindles resulting in growth arrest at 37 degrees Celsius; when associated with T-117.
Probable UTP--glucose-1-phosphate uridylyltransferase
Translation machinery-associated protein 17
Decreases the turnover number 145-fold. Decreases the turnover number 700-fold; when associated with Gln-96.
Decreases the turnover number 28-fold. Decreases the turnover number 700-fold; when associated with Met-77.
Saccharopine dehydrogenase [NAD(+), L-lysine-forming]
Impairs dsDNA and ssDNA binding of the MEC1-LCD1 complex.
Abolishes insertion into mitochondrial membranes.
Greatly reduced expression. Mislocalization to mitochondrial matrix.
Slightly reduced expression. Does not affect mitochondrial membrane localization but shows weaker membrane association.
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial
Putative uncharacterized protein YLR173W
Disrupts interaction with HRT1 and prevents RUB1/NEDD8 modification.
Decreases Mg(2+) import into mitochondria.
Increases Mg(2+) import into mitochondria.
Magnesium transporter MRS2, mitochondrial
V-type proton ATPase subunit a, Golgi isoform
54S ribosomal protein L20, mitochondrial
Abolishes membrane association. Lethal; when associated with S-205.
Abolishes membrane association. Lethal; when associated with S-206.
Decreases GTP binding and GTP hydrolysis.
Decreases GTP binding and increases GTP hydrolysis.
Locks YPT1 in the GTP-bound form by reducing GTP hydrolysis rate 40-fold.
Deoxyribodipyrimidine photo-lyase, mitochondrial
Reduces substrate binding 100-fold. Reduces quantum yield for dimer photolysis 3-fold.
54S ribosomal protein L41, mitochondrial
Putative RNA-binding protein involved in heterochromatin assembly
Ankyrin repeat-containing protein YCR051W
Abolishes ability to control levels of proteins with 'non-stop'.
Acetylornithine aminotransferase, mitochondrial
Uncharacterized membrane glycoprotein YNR065C
Abolishes substrate binding. Unable to confer thermotolerance. Reduces ATP hydrolysis by 98%; when associated with T-315. Completely abolishes ATPase activity; when associated with T-620.
Abolishes the ability to refold aggregated protein in vitro and to provide thermotolerance in vivo.
Almost completely abolishes ATP hydrolysis at NBD2, but does not affect nucleotide binding, thus keeping NBD2 in an ATP-bound state. Reduces stimulation of ATP hydrolysis upon substrate binding.
Can oligomerize in the absence of nucleotides.
Confers resistance to prion-curing by guanidine.
Impairs oligomerization at low protein concentrations.
Impairs oligomerization at low protein concentrations. Reduces ATP hydrolysis rate. Unable to confer thermotolerance. Completely abolishes ATPase activity; when associated with T-218.
Impairs prion propagation, but does not affect thermotolerance.
In HSP104(TRAP); completely abolishes ATP hydrolysis, but does not affect nucleotide binding, thus keeping HSP104 in an ATP-bound state; when associated with Q-285.
In HSP104(TRAP); completely abolishes ATP hydrolysis, but does not affect nucleotide binding, thus keeping HSP104 in an ATP-bound state; when associated with Q-687.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-778 and A-782.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-778 and A-789.
In NLS17KA; fails to concentrate in the nucleus; when associated with A-782 and A-789.
Increases basal level of ATPase activity and abolishes stimulation of ATP hydrolysis upon substrate binding. Inhibits growth at 37 degrees Celsius.
Largely reduces ATP hydrolysis. Alters bud morphology and causes septin mislocalization; when associated with I-499.
Reduces ATP and ADP binding at NBD2 6-fold, but does not affect ATP hydrolysis at NBD2. Reduces catalytic rate at NBD1.
Reduces ATP hydrolysis by 50%. Alters bud morphology and causes septin mislocalization; when associated with S-217.
Reduces ATP hydrolysis by 98%; when associated with T-218.
Reduces ATP hydrolysis, but does not affect oligomerization.
Reduces ATPase activity by 80%. Impairs oligomerization.
Reduces rate of ATP hydrolysis at NBD1 nearly 10-fold. No effect on oligomerization.
Site-specific fluorescent probe in an otherwise Trp-less HSP104. Fluorescence of this Trp changes in response to ATP and ADP binding at NBD2. Has no effect on ATP hydrolysis or protein stability.
1-phosphatidylinositol 3-phosphate 5-kinase FAB1
Cells are defective for growth at 38 degrees Celsius and show single-lobed, enlarged vacuoles.
Cells are defective for growth at 38 degrees Celsius.
Cells are defective for growth at 38 degrees Celsius. PtdIns(3,5)P2 levels are severely reduced.
Cells show growth at 38 degrees Celsius. Failure to localize to the vacuole membrane.
Impairs intramolecular interaction with the CCR domain, leading to a hyperactive kinase mutant.
Impairs intramolecular interaction with the CCR domain, leading to a hyperactive kinase mutant; elevates PI(3,5)P2 levels.
Impairs intramolecular interaction with the kinase domain. Leads to elevated PI(3,5)P2 levels.
Loss of interaction with VAC14. Failure to localize to the vacuole membrane.
Interaction with SRP1; when associated with missing 309-I--E-663.
Mislocalized to the endoplasmic reticulum.
No interaction with SRP1; when associated with missing 309-I--E-663.
No interaction with SRP1; when associated with missing 309-I--E-663. Mislocalized to the endoplasmic reticulum.
Loss of interaction with ATG18 and VAC7.
Loss of interaction with ATG18, FAB1 and VAC7. No loss of interaction with FIG4.
Vacuole morphology and inheritance protein 14
In FIG4-1; loss of catalytic activity in vitro. Almost 3-fold increase in PtdIns(3,5)P2 level in vivo. Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover.
Partially defective in both hyperosmotic shock-induced PtdIns(3,5)P2 elevation and turnover.
Ethanolamine-phosphate cytidylyltransferase
Putative uncharacterized protein YCL021W-A
In POL5dn; has no DNA synthesis activity, but complements the lethality of a pol5 null mutant; when associated with N-623.
In POL5dn; has no DNA synthesis activity, but complements the lethality of a pol5 null mutant; when associated with N-625.
Abolished binding to phosphatidylserine.
Does not affect binding to phosphatidylserine.
Strongly reduced binding to phosphatidylinositol 4-phosphate (PI4P). Does not affect binding to phosphatidylserine.
Strongly reduced binding to phosphatidylserine and phosphatidylinositol 4-phosphate (PI4P).
Strongly reduced binding to phosphatidylserine.
Lethal; Enzyme activity 10% of wild-type.
Non-viable; reduced enzyme activity to 8% of wild-type.
Still viable; activity near to wild-type.
Still viable; little change in enzyme activity.
Still viable; slow growth; near to wild-type enzyme activity.
Putative uncharacterized protein YHR214C-E
Transcription initiation factor TFIID subunit 6
Superoxide dismutase [Mn], mitochondrial
Chromatin structure-remodeling complex protein RSC58
Chromatin structure-remodeling complex subunit RSC1
Dihydrosphingosine 1-phosphate phosphatase YSR3
Abolishes interaction with OST3 and STT3; disrupts interaction between OST3 and STT3.
Disrupts interaction between OST3 and STT3.
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4
No effect on interaction between OST3 and STT3.
Severe growth defect; abolishes interaction with OST3 and STT3.
Severe growth defect; abolishes interaction with OST3.
Severe growth defect; abolishes interaction with OST3; disrupts interaction between OST3 and STT3.
Mediator of RNA polymerase II transcription subunit 3
In pmr3-1; decreases nuclear fusion efficiency.
In pmr3-2; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-3; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-5; leads to mislocalization and decreases nuclear fusion efficiency.
In pmr3-7; leads to mislocalization and decreases nuclear fusion efficiency when temperature rises.
In pmr3-8; leads to mislocalization and decreases nuclear fusion efficiency.
Mitochondrial inner membrane protein SHH3
Methionyl-tRNA formyltransferase, mitochondrial
Mitochondrial inner membrane protein SHH4
Loss of kinase activity and no vacuolar carboxypeptidase Y (PCR1) sorting to the vacuole.
Ubiquitin carboxyl-terminal hydrolase 10
Leads to increased oxidoreductase activity.
Impairs interaction with COP1 and SEC21 and mislocalizes to the vacuole.
Impairs interaction with COP1, SEC21 and SEC23 and loss of reticulum endoplasmic exit.
Altered inheritance of mitochondria protein 39, mitochondrial
Y' element ATP-dependent helicase YPR204W
Impairs interaction with ATG8 and decreases subsequent reticulophagy; when associated with A-245.
Serine/threonine-protein phosphatase PP-Z1
Abolished kinase activity. Loss of cell viability under starvation.
Enables the binding of the inhibitor of the kinase activity 1-NA-PP1.
Impairs autophagic activity; when associated with A-508.
Impairs autophagic activity; when associated with A-515.
Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-429.
Impairs interaction with ATG8 and decreases autophagy efficiency; when associated with A-432.
Leads to constitutive autophosphorylation activity.
Loss of cell viability under starvation.
Catalytically inactive. Internalization defects.
No kinase activity. ATP-binding defect. Internalization defects.
No phosphorylation. Reduced sphingolipid biosynthesis inhibitor drug myriocin (ISP-1) resistance. No defect in growth or endocytosis.
No phosphorylation; when associated with A-504 or D-504.
No phosphorylation; when associated with A-662 or D-662.
Reduced sphingolipid biosynthesis inhibitor drug myriocin (ISP-1) resistance. Defective in growth and endocytosis.
Affects protein stability, but has no effect on peptidase activity.
Temperature sensitive; abolishes proteolytic activity for RIP1, but not for COX4.
Abolishes interaction with SBA1. Reduces client protein activation activity, leading to growth defect at high temperatures.
Causes a 90% reduction in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Causes a 98% reduction in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Decreases AHA1 binding affinity and substantially reduces client protein activation activity.
Decreases AHA1 binding affinity, but has no effect on client protein activation activity.
Enhances ATPase activity and client protein activation.
Enhances homodimerization, ATPase activity and client protein activation.
Induces a 6-fold increase in ATPase activity and a reduced client protein activation activity, leading to growth defect at high temperatures.
Induces a 6-fold increase in ATPase activity.
Induces a loss of ATPase activity. Can be reactivated by AHA1.
Induces a total loss of function at 34 degrees Celsius. Abolishes interaction with SBA1.
No effect on ATPase activity. Reduces client protein activation activity, leading to growth defect at high temperatures.
Reduces ATPase activity and client protein activation.
Reduces client protein activation activity, leading to growth defect at high temperatures.
Reduces client protein activation activity. Resistant to ATPase activation by AHA1.
Reduces client protein activation activity; when associated with K-579.
Reduces client protein activation activity; when associated with T-576.
Reduces homodimerization, ATPase activity and client protein activation.
Specifically reduces the activation of the exogenous ligand glucocorticoid receptor.
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Vacuolar protein-sorting-associated protein 24
No interaction with FIS1; slight decrease in interaction with DNM1.
Results in mitochondrial division defect, but has no effect on the interaction with DNM1.
Slight decrease in interaction with DNM1.
Slight decrease in interaction with FIS1; 20-fold decrease in interaction with DNM1; even distribution along mitochondria not punctate.
Suppresses the mitochondrial fission defect of a FIS1-3 mutant by affecting interaction with FIS1.
Dolichyl-phosphate-mannose--protein mannosyltransferase 5
Uncharacterized membrane protein YJL163C
Reduces ability to activate transcription and to induce invasive growth.
Vacuolar protein sorting-associated protein 45
Loss of interaction with SEC23/SEC24 complex.
Chromosome transmission fidelity protein 18
ADP-ribosylation factor GTPase-activating protein effector protein 1
Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]
CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4
DNA damage checkpoint control protein MEC3
54S ribosomal protein L22, mitochondrial
D-arabinose dehydrogenase [NAD(P)+] heavy chain
Membrane-anchored lipid-binding protein LAM6
Regulator of Ty1 transposition protein 103
Moderately reduces hydrolysis, and causes a 10-fold reduction in transglycosylation activity.
Topoisomerase I damage affected protein 11
No phosphorylation; when associated with A-319.
No phosphorylation; when associated with A-320.
No phosphorylation; when associated with A-322.
No phosphorylation; when associated with A-323.
Sensitive to alkali. Fails to restore normal levels of SLT2 phosphorylation upon alkaline stress.
Endoplasmic reticulum transmembrane helix translocase
DNA replication complex GINS protein PSF2
Uncharacterized membrane protein YOL019W
U3 small nucleolar RNA-associated protein 8
Uncharacterized WD repeat-containing protein YNL035C
Mitogen-activated protein kinase SLT2/MPK1
Truncated non-functional calcium-binding mitochondrial carrier SAL1-1
Probable pseudouridine-5'-phosphatase YKL033W-A
Mitochondrial aspartate-glutamate transporter AGC1
Peptidyl-prolyl cis-trans isomerase C, mitochondrial
Strongly reduces in vitro PPIase activity and in vivo protein folding activity. Reduces binding to cyclosporin A 2-fold.
Serine/threonine-protein kinase PTK2/STK2
U1 small nuclear ribonucleoprotein component PRP42
Glutathione peroxidase-like peroxiredoxin 2
Abolishes histone acetylase activity. Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Decreases activity stimulation by VPS75.
Decreases binding and activity stimulation by VPS75.
Decreases binding and activity stimulation by VPS75. Decreases acetylation of histone H3 'Lys-9' and 'Lys-27'.
Decreases histone acetylase activity. Decreases expression (at protein level). Increases sensitivity to methyl methane sulfonate and hydroxyurea (genotoxic stress).
Decreases histone acetylase activity. Increases sensitivity to methyl methane sulfonate and hydroxyurea (genotoxic stress).
Decreases histone acetylase activity; when associated with A-211.
Decreases histone acetylase activity; when associated with A-222.
Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Mildly increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Normal histone acetylase activity. Increases sensitivity to methyl methane sulfonate, camptothecin and hydroxyurea (genotoxic stress).
Y' element ATP-dependent helicase YFL066C
Serine/arginine (SR)-type shuttling mRNA binding protein NPL3
General transcription and DNA repair factor IIH helicase subunit XPB
At 37 degrees, growth slows after 6 to 8 hours and cell division stops after 20 hours.
Reduced growth rate at all temperatures; when associated with A-333.
Reduced growth rate at all temperatures; when associated with R-385.
54S ribosomal protein L44, mitochondrial
DNA mismatch repair protein MSH1, mitochondrial
Increases the rate of point mutations 19-fold and displays a moderate decrease in respiration-competent cells during a time course.
Increases the rate of point mutations 35-fold and displays a significant decrease in respiration-competent cells during a time course.
Slightly increases the frequency of point mutations and repeat-mediated deletion rates in mtDNA.
Low-affinity phosphate transporter PHO90
3-oxoacyl-[acyl-carrier-protein] synthase homolog
In FUR1-5; causes resistance to 5-fluorouracil (5FU).
In FUR1-7; causes resistance to 5-fluorouracil (5FU).
In FUR1-8; causes resistance to 5-fluorouracil (5FU).
In FUR1-9; causes resistance to 5-fluorouracil (5FU).
Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating
In TEL1-11; short telomere phenotype and impairs DNA-damage checkpoint function at 37 degrees Celsius.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with D-2611-2612-A and E-2631.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with D-2611-2612-A and K-2616.
Short telomere phenotype in vivo and impairs kinase activity in vitro; when associated with K-2616 and E-2631.
1-acyl-sn-glycerol-3-phosphate acyltransferase
Near wild-type activity for deacetylation. Increases slightly the KM for NAD(+) to 25 uM.
Nearly or completely catalytically inactive.
Low molecular weight phosphotyrosine protein phosphatase
Ceramide very long chain fatty acid hydroxylase SCS7
Confers resistance to Syringomycin E, showing impaired catalytic activity.
Maintains the susceptibility to Syringomycin E, showing no effect on catalytic activity.
Reduces the susceptibility to Syringomycin E, showing reduced catalytic activity.
UDP-N-acetylglucosamine pyrophosphorylase
Vacuolar import and degradation protein 27
Abolishes interaction with VPS4. Impairs sorting.
Abolishes RFB-binding, replication fork blocking activity and reduces recombination rate.
DNA replication fork-blocking protein FOB1
Abolishes interaction with the CIA complex. No effect on interaction with YAE1. Strongly decreases iron incorporation into RLI1.
Decreases interaction with YAE1 and the CIA complex. Abolishes iron incorporation into RLI1.
Decreases interaction with YAE1 and the CIA complex. Strongly decreases iron incorporation into RLI1.
Lethal at 37 degrees Celsius; when associated with D-57, D-103 and G-134.
Lethal at 37 degrees Celsius; when associated with G-11, E-83, L-113 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, L-94, S-101 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, E-83 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, E-83 and L-113.
Lethal at 37 degrees Celsius; when associated with G-11, P-19, L-113 and G-147.
Lethal at 37 degrees Celsius; when associated with G-11, R-50, L-94 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, R-50, S-101 and S-146.
Lethal at 37 degrees Celsius; when associated with G-11, R-50,L-94 and S-101.
Lethal at 37 degrees Celsius; when associated with L-18, D-103 and G-134.
Lethal at 37 degrees Celsius; when associated with L-18, D-57 and D-103.
Lethal at 37 degrees Celsius; when associated with L-18, D-57 and G-134.
Lethal at 37 degrees Celsius; when associated with P-19, E-83, L-113 and G-147. Lethal at 37 degrees Celsius; when associated with R-0, R-50, L-94, S-101 and S-146.
No effect on interaction with YAE1 or the CIA complex. No effect on iron incorporation into RLI1.
Slightly decreases interaction with YAE1 and the CIA complex. No effect on iron incorporation into RLI1.
Increases ability to transport iron and decreases ability to transport cobalt.
Gives rise to unstable commitment complexes.
Transcription initiation factor TFIID subunit 12
Proton acceptor; for glutaminase activity
Increased recombination centers protein 6
Reduced viability upon starvation protein 167
Cytochrome c oxidase subunit 5B, mitochondrial
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
Ribosome-recycling factor, mitochondrial
Does not confer ability to stimulate the ATPase activity of mtHSP70.
Mitochondrial import inner membrane translocase subunit TIM16
Decrease of ER lumenal misfolded protein degradation.
Decrease of ER lumenal misfolded protein degradation. No effect on interaction with CDC48, HRD3, KAR2, UBX2, HRD1 or EMP47.
Abolishes kinase activity and termination by RNA polymerase I.
Multifunctional tryptophan biosynthesis protein
Putative uncharacterized protein YGL262W
Abolished fumarate hydratase activity and ability to participate in DNA repair.
Abolishes processing by the mitochondrial processing peptidase. Mainly localizes in the cytosol, with a small fraction in the mitochondrion. Reduced fumarate hydratase activity.
Does not affect processing by the mitochondrial processing peptidase. Localizes both in the mitochondrion and cytosol. Exhibits high fumarate hydratase activity.
Abolishes E3 activity, strongly reduces zinc binding and destabilizes the protein, but no effect on subcellular location.
Impairs the peroxisomal localitation and leads to both nuclear and cytosolic localization.
In strain: Awamori-1, AKU-4011, K1, K5, NRIC 23, NRIC 1413 and NRIC 1685.
Uncharacterized membrane protein YIL054W
Antisense of depressing factor protein 1
No cell growth and 2-fold decrease in ATPase activity in vitro.
No cell growth and drastic decrease in ATPase activity in vitro.
Iron sulfur cluster assembly protein 2, mitochondrial
Chromosome transmission fidelity protein 8
Cytoplasmic 60S subunit biogenesis factor REI1
54S ribosomal protein RTC6, mitochondrial
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Uncharacterized RNA-binding protein YGR250C
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
Altered inheritance of mitochondria protein 21
Rho1 guanine nucleotide exchange factor TUS1
Strongly reduces interaction with HSP70 family members.
Endoplasmic reticulum transmembrane protein 1
Heterotrimeric G protein gamma subunit GPG1
C-1-tetrahydrofolate synthase, mitochondrial
Abolishes interaction with ELP1/IKI3 and ELP3.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-628 and A-654.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-628 and A-675.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-626, A-654 and A-675.
Dramatically reduced interaction with microtubules but no effect on interaction with ELP1/IKI3 or ELP3; when associated with A-628, A-654 and A-675.
Abolished binding to acetylated histone H3.
Abolished binding to acylated histone H3.
Does not affect ability to bind crotonylated lysines, while abolishing binding to acetylated lysines.
Does not affect binding to acylated histone H3.
Transcription initiation factor TFIID subunit 14
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Vacuolar protein sorting-associated protein 54
Abolishes autopalmitoylation and VAC8 palmitoylation.
Actin-related protein 2/3 complex subunit 3
Uncharacterized membrane protein YBR220C
Digitonin sensitive, reduces structural integrity of the protein.
Digitonin sensitive, reduces structural integrity of the protein. Affects the substrate specificity.
Digitonin sensitive, reduces structural integrity of the protein. Greatly inhibits the fatty acid elongation cycle. Displays a higher KM and lower Vmax values.
Exhibits a moderate fatty acid elongation defect.
Greatly inhibits the fatty acid elongation cycle. Displays normal KM but reduced Vmax values.
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PHS1
Maintenance of telomere capping protein 6
Cyclin-dependent kinases regulatory subunit
N-acetyl-gamma-glutamyl-phosphate reductase
Sulfite reductase [NADPH] flavoprotein component
In RIM20-292; prevents cleavage of RIM101.
pH-response regulator protein palA/RIM20
In YME2-4; prevents mtDNA escape, growth on nonfermentable carbon sources and growth at 37 degrees Celsius on glucose.
Uncharacterized mitochondrial carrier YPR011C
Biogenesis of lysosome-related organelles complex 1 subunit KXD1
Uncharacterized peptide chain release factor-like protein YLR281C, mitochondrial
Endosomal/vacuolar adapter protein YPT35
Impairs autophosphorylation and mating efficiency.
Impairs interaction with BEM1; when associated with A-477.
Impairs interaction with BEM1; when associated with G-475.
Impairs phosphorylation of STE11 and histone H2B and mating efficiency.
Chromatin structure-remodeling complex protein RSC6
Phenylalanine--tRNA ligase, mitochondrial
DNA-directed RNA polymerases I, II, and III subunit RPABC4
U3 small nucleolar RNA-associated protein MPP10
Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.
Suppressor of lethality of KEX2 GAS1 double null mutant protein 1
Prevents relocalization to the vacuolar membrane.
Cannot restore cell growth in USB1 depleted cells.
Does not affect growth phenotype. Significantly decreases in vitro processing.
U3 small nucleolar ribonucleoprotein protein IMP4
Abolished acetylation by ESA1, leading to decreased diacylglycerol synthesis; when associated with R-496.
Abolished acetylation by ESA1, leading to decreased diacylglycerol synthesis; when associated with R-801.
Impairs membrane recruitiment; when associated with A-11 and A-15.
Impairs membrane recruitiment; when associated with A-8 and A-11.
Impairs membrane recruitiment; when associated with A-8 and A-15.
Impairs phosphatidate phosphatase activity.
High-osmolarity-induced transcription protein 1
Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410.
Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410.
Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410.