Barely detectable pre-crRNA cleavage, retains target RNA cleavage.
CRISPR-associated endoribonuclease Cas13a
Complete loss of pre-crRNA cleavage, retains target RNA cleavage. Decreased ability to activate non-specific RNA degradation when tested with a 6-spacer CRISPR array.
Dramatically decreased cleavage of target RNA.
No cleavage of target RNA, retains pre-crRNA cleavage. Still no effect on pre-crRNA cleavage; when associated with 1048-A--A-1053.
No cleavage of target RNA, retains pre-crRNA cleavage. Still no effect on pre-crRNA cleavage; when associated with 427-A--A-477.
Interaction with polymerase core subunit RpoC
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
Phosphocysteine intermediate; for EIIB activity
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
LPXTG cell wall anchor domain-containing protein
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Cysteine sulfenic acid (-SOH) intermediate
Toxin-antitoxin system YwqK family antitoxin
Schiff-base intermediate with D-ribose 5-phosphate
O-(5'-phospho-DNA)-tyrosine intermediate
serine-type D-Ala-D-Ala carboxypeptidase
PTS system alpha-glucoside-specific EIICB component
AB hydrolase-1 domain-containing protein
ABC transporter substrate-binding protein
Flavodoxin-like domain-containing protein
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Curli production assembly/transport component CsgE
Schiff-base intermediate with dihydroxyacetone-P
Gly-cisPro motif, important for rejection of L-amino acids