Transcription antitermination protein NusB
4-hydroxyphenylacetate 3-monooxygenase oxygenase component
Extracytoplasmic solute receptor protein
Putative membrane protein insertion efficiency factor
3-phosphoshikimate 1-carboxyvinyltransferase
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
4-hydroxy-4-methyl-2-oxoglutarate aldolase
O-(5'-phospho-DNA)-tyrosine intermediate
Important for interaction with partner proteins
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
DNA-directed RNA polymerase subunit omega
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
Outer membrane lipoprotein carrier protein LolA
Uncharacterized PIN and TRAM-domain containing protein TTHA0540
DNA-directed RNA polymerase subunit alpha
4-hydroxy-tetrahydrodipicolinate synthase
Ribosomal RNA small subunit methyltransferase F
Phosphoribosylformylglycinamidine synthase subunit PurL
10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Ribosomal RNA small subunit methyltransferase G
tRNA (adenine(58)-N(1))-methyltransferase TrmI
3-methyl-2-oxobutanoate hydroxymethyltransferase
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx.
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with A-250.
Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with N-226.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+) and Ca(2+).
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-304 and A-307.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-304 and A-383.
Does not affect Mg(2+) transport, but increases permeability for Mn(2+); when associated with A-307 and A-383.
Does not affect activity. Increases Ni(2+) sensitivity.
Still possesses a slight channel activity.
Probable 2-phosphosulfolactate phosphatase
Interaction with polymerase core subunit RpoC
Probable dual-specificity RNA methyltransferase RlmN
Single-stranded-DNA-specific exonuclease RecJ
2-oxoisovalerate dehydrogenase subunit beta
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Probable nicotinate-nucleotide adenylyltransferase
ATP-dependent Clp protease proteolytic subunit
UDP-N-acetylenolpyruvoylglucosamine reductase
UPF0173 metal-dependent hydrolase TTHA1283
3-isopropylmalate dehydratase large subunit
Ribosomal RNA small subunit methyltransferase B
Decreases amount and stability of iron-sulfur center 4.
Loss of XDG activity, but retains much of its UDG and HDG activities.
Loss of XDG activity. Decrease in UDG and HDG activities.
Loss of XDG activity. Strong decrease in UDG and HDG activities.
Loss of xanthine DNA glycosylase (XDG) activity. Decrease in uracil DNA glycosylase (UDG) and hypoxanthine DNA glycosylase (HDG) activities. Shows low activity for the removal of uracil from U/G or thymine from T/G.
Minimal effect on UDG and XDG activities. Strong reduction on HDG activity on A/I and T/I base pairs.
Strong decrease in UDG, HDG and XDG activities.
4-hydroxyphenylacetate 3-monooxygenase, reductase component
ATP-dependent Clp protease ATP-binding subunit ClpX
Probable glycine dehydrogenase (decarboxylating) subunit 2
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
ATP phosphoribosyltransferase regulatory subunit
Bifunctional oligoribonuclease and PAP phosphatase NrnA
Decreased substrate affinity (KM 420 uM) and catalytic activity for a ssDNA 6-mer.
Fibronectin type-III domain-containing protein
1,4-alpha-glucan branching enzyme TTHA1902
Almost complete loss of branching activity but 10-fold increase in hydrolytic activity.
Complete loss of enzymatic activity. Binds amylose but not glycogen.
Schiff-base intermediate with acetaldehyde
Glutamyl-tRNA(Gln) amidotransferase subunit C
No effect on protease activity against alpha-casein.
Strongly reduces interaction with DNA. Reduces CPD repair activity by 80%.
[LysW]-aminoadipate semialdehyde transaminase
DNA-directed RNA polymerase subunit beta'
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Pyridoxal 5'-phosphate synthase subunit PdxT
Chromosomal replication initiator protein DnaA
No effect on the orientation of the bound flavin.
Resolvase/invertase-type recombinase catalytic
Ribosomal RNA large subunit methyltransferase H
Glutamate-1-semialdehyde 2,1-aminomutase
DNA-directed RNA polymerase subunit beta
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in TtuB)
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Involved in ionization of N3 of dUMP, leading to its activation
Carbamoyl-phosphate synthase small chain
Probably phosphorylated, more strongly associated with the 50S ribosomal subunit.
N-[TS] motif that is essential for LysX substrate specificity
Redox-sensing transcriptional repressor Rex
Further decreases Ptase activity in E.coli hybrid ribosomes. Significantly decreases binding of a tRNA analog to the A or P site.
Increases Ptase activity of hybrid ribosomes to a value greater than that of pure E.coli ribosomes. Increases binding of a tRNA analog to the P site.
No effect on Ptase activity of hybrid ribosomes. No change in binding of a tRNA analog to the A or P site.
Restores Ptase activity of hybrid ribosomes to that of pure E.coli ribosomes. Significantly increases binding of a tRNA analog to the P site.
Gly-cisPro motif, important for rejection of L-amino acids
Ribosomal RNA small subunit methyltransferase A
3-isopropylmalate dehydratase small subunit
5-glutamyl 2-aminoadipic acid; alternate
Disrupts lysine biosynthesis. Lysine-auxotrophic phenotype.
Carbamoyl-phosphate synthase large chain
Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Little change in the aminoacylation activity.
Slightly reduces the aminoacylation activity.
Carboxypeptidase regulatory-like domain-containing protein
Phosphate import ATP-binding protein PstB
Ribosomal protein S12 methylthiotransferase RimO
Transcription termination/antitermination protein NusA
Glutamine synthetase and cystathionine beta-lyase binding protein
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Large decrease in activity and a small decrease in substrate affinity.
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
tRNA (guanosine(18)-2'-O)-methyltransferase
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Ribosomal RNA small subunit methyltransferase H
WD_REPEATS_REGION domain-containing protein
Transcription termination/antitermination protein NusG
Great decrease in chaperone activity. Retains ATPase activity and oligomerization.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-167.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-350.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-353.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-355.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-464.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-467.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-475.
Great decrease in chaperone activity. Retains ATPase activity and oligomerization; when associated with C-520.
Loss of ability to bind ATP. Residual ATPase activity.
No effect on ability to bind ATP. Decrease in ATPase activity.
No effect on ability to bind ATP. Increase in ATPase activity.
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
Increases the channeling efficiency of propanaldehyde from 57% to 94%.
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Type III-B CRISPR module RAMP protein Cmr4
CRISPR-associated endonuclease/helicase Cas3
Crossover junction endodeoxyribonuclease RuvC
Polyribonucleotide nucleotidyltransferase
Cytochrome c-type biogenesis protein CcmE
Helical; Signal-anchor for type II membrane protein
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Peptide methionine sulfoxide reductase MsrA
Phosphoribosylformylglycinamidine synthase subunit PurQ
Has some defects in posttransfer editing activity.
No change in posttransfer editing activity.
Phenylalanine--tRNA ligase alpha subunit
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
Lactate-binding periplasmic protein TTHA0766
Holliday junction ATP-dependent DNA helicase RuvB
Nucleophile; cysteine thiosulfonate intermediate
8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase
Probable transcriptional regulatory protein TTHA0821
Glutamyl-tRNA(Gln) amidotransferase subunit A
Imidazole glycerol phosphate synthase subunit HisF
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Efflux RND transporter periplasmic adaptor subunit
64% loss of cleavage activity (PubMed:21572442); 99% loss of cleavage activity (PubMed:21572444).
99.8% loss of cleavage activity, binds RNA normally.
No effect on cleavage, increases enzyme turnover.
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Holliday junction ATP-dependent DNA helicase RuvA
2-oxoisovalerate dehydrogenase subunit alpha