2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Ribosomal RNA large subunit methyltransferase E
GMP synthase [glutamine-hydrolyzing] subunit B
Abolishes peptidase activity, but retains ATPase activity.
Almost completely abolishes ATPase activity and severely reduces proteolytic activity.
Completely abolishes ATPase activity and makes peptidase activity independent on ATP stimulus.
Decreases ATPase activity. Completely abolishes peptidase activity in the absence of ATP, but ATP-stimulated peptidase activity i close to wild-type levels.
Retains 5% ATPase activity. Proteolytic activity is greatly impaired and cannot be stimulated by nucleotides.
Severely reduces ATPase and proteolytic activity.
DNA double-strand break repair Rad50 ATPase
Both peptidolytic and proteolytic activities doubled, probably due to the increase of the diameter of the channel for product exit. Retains less than 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the exit channel and impairs product exit from the catalytic chamber.
Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-414.
Decreased catalytic activity towards protein substrates. Retains 10% of wild-type activity towards casein and about 30% towards oxidized insulin beta chain. However, there is no change in the activity for a tripeptide substrate.
Retains 50% of wild-type activity after modification of the thiol group by maleimide, which decreases the diameter of the access channel and impairs substrate access to the active site. Decreased catalytic activity towards fluorogenic substrate and insulin beta chain prior to any modification or oxidation, probably due to the decrease of the diameter of the substrate access channel. Further decreased catalytic activity towards these substrates after modification with fluorescein-5-maleimide or oxidation by oxidized glutathione; when associated with C-643.
tRNA (guanine(26)-N(2))-dimethyltransferase
Glutamyl-tRNA(Gln) amidotransferase subunit E
Probable 2-phosphosulfolactate phosphatase
Translation initiation factor 2 subunit alpha
Resolvase/invertase-type recombinase catalytic
DNA-directed RNA polymerase subunit Rpo11
Phosphoribosylformylglycinamidine synthase subunit PurL
Schiff-base intermediate with acetaldehyde
Glyceraldehyde-3-phosphate dehydrogenase
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Putative nicotinate phosphoribosyltransferase
tRNA (cytidine(56)-2'-O)-methyltransferase
Probable glycine dehydrogenase (decarboxylating) subunit 2
Putative HTH-type transcriptional regulatory protein Ta1363
Carbamoyl-phosphate synthase small chain
DNA double-strand break repair protein Mre11
DNA-directed RNA polymerase subunit Rpo3
No effect. Strongly reduced ribonuclease activity; when associated with A-21.
No effect. Strongly reduced ribonuclease activity; when associated with A-22.
Phosphoribosylformylglycinamidine synthase subunit PurQ
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Phenylalanine--tRNA ligase alpha subunit
DNA-directed RNA polymerase subunit Rpo1N
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
GMP synthase [glutamine-hydrolyzing] subunit A
Potassium-transporting ATPase potassium-binding subunit
Nicotinamide-nucleotide adenylyltransferase
Succinate dehydrogenase hydrophobic membrane anchor subunit
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Putative sulfur carrier protein Ta1170/Ta1414
Uncharacterized solute-binding protein Ta1052
O-(3'-phospho-DNA)-tyrosine intermediate
3-phosphoshikimate 1-carboxyvinyltransferase
Probable cobalamin biosynthesis protein CobD
Glutamyl-tRNA(Gln) amidotransferase subunit D
Aspartate carbamoyltransferase regulatory chain
Does not affect stimulation of helicase activity.
D-glyceraldehyde dehydrogenase (NADP(+))
DNA-directed RNA polymerase subunit Rpo1C
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Cobalt-precorrin-5B C(1)-methyltransferase
Ornithine carbamoyltransferase, catabolic
Adenosylcobinamide-GDP ribazoletransferase
Probable glycine dehydrogenase (decarboxylating) subunit 1
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Digeranylgeranylglycerophospholipid reductase
Removed in mature form; by autocatalysis
DNA-directed RNA polymerase subunit Rpo10
O-(5'-phospho-DNA)-tyrosine intermediate
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Bifunctional methyltransferase/endonuclease
Probable D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase
4.6-fold decrease in the catalytic efficiency for ATP, with only a marginal decrease in affinity compared to the wild-type.
Strong decrease of the affinity for ATP and 17-fold decrease of the catalytic efficiency compared to the wild-type.
Strong decrease of the affinity for ATP, but almost the same catalytic efficiency compared to the wild-type.
Vitamin B12-dependent ribonucleoside-diphosphate reductase
DNA-directed RNA polymerase subunit Rpo6
Glutamate-1-semialdehyde 2,1-aminomutase
Mevalonate 3,5-bisphosphate decarboxylase
DNA-directed RNA polymerase subunit Rpo5
Translation initiation factor 2 subunit gamma
Nascent polypeptide-associated complex protein
Pyridoxal 5'-phosphate synthase subunit PdxT
Carbamoyl-phosphate synthase large chain
Phosphoribosylformylglycinamidine cyclo-ligase
Cysteine sulfenic acid (-SOH) intermediate
Ribosomal RNA small subunit methyltransferase Nep1
Signal recognition particle 54 kDa protein
Probable cyclic pyranopterin monophosphate synthase
Markedly increases peptidolytic activity. Designated open-gate mutant.
No effect on PAN's ability to stimulate gate opening.
Prevents PAN to associate with the proteasome and stimulate gate opening.
UPF0173 metal-dependent hydrolase Ta0764
Bifunctional phosphopantetheine adenylyltransferase/NTP phosphatase
DNA repair and recombination protein RadB
Signal recognition particle receptor FtsY
Translation initiation factor 2 subunit beta
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
DNA-directed RNA polymerase subunit Rpo2
Sugar fermentation stimulation protein homolog
Probable translation initiation factor IF-2