GMP synthase [glutamine-hydrolyzing] subunit A
[LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
Proton acceptor; for processing activity
Schiff-base intermediate with substrate; via pyruvic acid
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Imidazole glycerol phosphate synthase subunit HisF
Probable glycine dehydrogenase (decarboxylating) subunit 2
Cysteine sulfenic acid (-SOH) intermediate
Phenylalanine--tRNA ligase alpha subunit
Uncharacterized solute-binding protein STK_20340
Methylated-DNA--protein-cysteine methyltransferase
Carbamoyl-phosphate synthase large chain
2-oxoacid:ferredoxin oxidoreductase 2, subunit beta
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)
DNA-directed RNA polymerase subunit Rpo12
tRNA N6-adenosine threonylcarbamoyltransferase
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)
UPF0173 metal-dependent hydrolase STK_14180
5-glutamyl 2-aminoadipic acid; alternate
Alpha-aminoadipate/glutamate carrier protein LysW
Probable glycine cleavage system H protein 1
Glutamyl-tRNA(Gln) amidotransferase subunit C
DNA-directed RNA polymerase subunit Rpo10
Aspartate carbamoyltransferase regulatory chain
tRNA (guanine(26)-N(2))-dimethyltransferase
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Translation initiation factor 2 subunit alpha
Imidazole glycerol phosphate synthase subunit HisH
DNA-directed RNA polymerase subunit Rpo3
Sugar fermentation stimulation protein homolog
Resolvase/invertase-type recombinase catalytic
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Membrane-associated ATPase epsilon chain
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Probable glycine cleavage system H protein 3
DNA double-strand break repair helicase HerA
Lack of ATPase and helicase activities. Decreases ssDNA-binding ability. Does not bind to blunt-ended dsDNA and Holliday junction substrate.
Strong decrease in ATPase and helicase activities.
Digeranylgeranylglyceryl phosphate synthase
Glutamyl-tRNA(Gln) amidotransferase subunit A
GF motif that is essential for ArgX substrate specificity
[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase
3-phosphoshikimate 1-carboxyvinyltransferase
Putative pterin-4-alpha-carbinolamine dehydratase
[LysW]-lysine/[LysW]-ornithine hydrolase
Involved in ionization of N3 of dUMP, leading to its activation
Glutamyl-tRNA(Gln) amidotransferase subunit D
Glutamyl-tRNA(Gln) amidotransferase subunit E
3-isopropylmalate dehydratase large subunit
DNA double-strand break repair protein Mre11
Kelch domain-containing protein STK_09390
Glyceraldehyde-3-phosphate dehydrogenase
Pyridoxal 5'-phosphate synthase subunit PdxT
Phosphoribosylformylglycinamidine cyclo-ligase
DNA-directed RNA polymerase subunit Rpo1C
1.3-fold increase in FBPase catalytic efficiency.
1.7-fold increase in FBPase catalytic activity and 5-fold decrease in FBP affinity, leading to a 2.7-fold decrease in FBPase catalytic efficiency.
1000-fold decrease in FBPase catalytic activity.
175-fold decrease in FBPase catalytic efficiency.
2-fold increase in FBP affinity and 10-fold decrease in FBPase catalytic activity, leading to a 5.5-fold decrease in FBPase catalytic efficiency.
2000-fold decrease in FBPase catalytic activity.
275-fold decrease in FBPase catalytic activity.
930-fold decrease in FBPase catalytic efficiency.
Fructose-1,6-bisphosphate aldolase/phosphatase
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Retains the FBPase activity, but loses the FBP aldolase activity.
Schiff-base intermediate with DHAP; for FBP aldolase activity
10-fold reduction in both RNase H and dsRNase activity.
20% reduction in RNase H activity, 40% reduction in dsRNase activity, partial loss of thermostability.
Loss of RNase H activity, loss of dsRNase activity. Does not complement E.coli double deletion mutant.
RNase H and dsRNase activity increase slightly, decreased thermostability; when associated with A-145.
RNase H and dsRNase activity increase slightly, decreased thermostability; when associated with A-58.
Retains RNase H activity, considerable loss of dsRNase activity.
Retains RNase H activity, loss of dsRNase activity. Complements E.coli double deletion mutant.
Signal recognition particle 54 kDa protein
Nicotinamide-nucleotide adenylyltransferase
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
DNA-directed RNA polymerase subunit Rpo11
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Translation initiation factor 2 subunit gamma
Phosphoglucosamine/phosphogalactosamine mutase
Translation initiation factor 2 subunit beta
[LysW]-aminoadipate/[LysW]-glutamate kinase
Probable translation initiation factor IF-2
Probable glycine dehydrogenase (decarboxylating) subunit 1
2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha
Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
Same oxidoreductase activity as the wild-type.
Carbamoyl-phosphate synthase small chain
2-oxoacid:ferredoxin oxidoreductase 1, subunit beta
Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
Phosphoribosylformylglycinamidine synthase subunit PurL
3-hydroxypropionyl-coenzyme A synthetase
DNA double-strand break repair Rad50 ATPase
Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase
Decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
Decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.
Decrease in GlcNAc-1-P UTase activity but increase in Glc-1-P UTase activity.
Decrease in GlcNAc-1-P UTase and Glc-1-P UTase activities.
Decreases GlcNAc-1-P UTase and Glc-1-P UTase activities.
Increases GlcNAc-1-P UTase activity. Decreases Glc-1-P UTase activity.
Increases GlcNAc-1-P UTase and Glc-1-P UTase activities.
Increases both GlcNAc-1-P UTase and Glc-1-P UTase activities.
Loss of GlcNAc-1-P UTase activity; when associated with N-80.
Loss of GlcNAc-1-P UTase activity; when associated with V-97.
Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.
No change in GlcNAc-1-P UTase activity. Shows 20% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 4.8 times. Does not affect thermostability.
No change in GlcNAc-1-P UTase activity. Shows 38% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 16.8 times. Significantly affects the thermostability of the entire protein.
Slight decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
Strong decrease in GalN-1-P AcTase activity and almost loss of GlcN-1-P AcTase activity.
Strong decrease in GalN-1-P AcTase activity and decrease in GlcN-1-P AcTase activity.
Strong decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
Strong decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.
Strong increase in GlcNAc-1-P UTase activity and decrease in Glc-1-P UTase activity.
N-[TS] motif that is essential for LysX substrate specificity
Probable cyclic pyranopterin monophosphate synthase
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
3-isopropylmalate dehydratase small subunit
3-methyl-2-oxobutanoate hydroxymethyltransferase
Formate-dependent phosphoribosylglycinamide formyltransferase
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Putative ABC transporter ATP-binding protein STK_11360
O-(3'-phospho-DNA)-tyrosine intermediate
Threonine--tRNA ligase catalytic subunit
Removed in mature form; by autocatalysis
Probable inosine/xanthosine triphosphatase
Metallo-beta-lactamase fold-containing protein ST1585
Ribosomal RNA small subunit methyltransferase Nep1
Cobalt-precorrin-5B C(1)-methyltransferase
Probable glycine cleavage system H protein 2
2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
DNA double-strand break repair nuclease NurA
Strong decrease in nuclease activity. Does not form a stable dimer.
GMP synthase [glutamine-hydrolyzing] subunit B
Adenosylcobinamide-GDP ribazoletransferase
Putative Tricorn-like protease C-terminal subunit
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
DNA-directed RNA polymerase subunit Rpo5