sn-glycerol-3-phosphate import ATP-binding protein UgpC
Concanavalin A-like lectin/glucanase superfamily protein
Transcription antitermination protein NusB
Heme-containing CO-sensing transcriptional regulator RcoM 2
AB hydrolase-1 domain-containing protein
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
O-(3'-phospho-DNA)-tyrosine intermediate
Ribosomal RNA small subunit methyltransferase H
Penicillin-binding protein activator LpoB
Putative phosphoenolpyruvate synthase regulatory protein
Ribulose bisphosphate carboxylase large chain
Probable transcriptional regulatory protein Bxeno_A1185
Cobalt-precorrin-5B C(1)-methyltransferase
Integrating conjugative element protein pill, pfgi-1
Phosphate import ATP-binding protein PstB
Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Taurine import ATP-binding protein TauB 2
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Probable septum site-determining protein MinC
N-succinylglutamate 5-semialdehyde dehydrogenase
Important for interaction with partner proteins
Crossover junction endodeoxyribonuclease RuvC
Putative membrane protein insertion efficiency factor
Proton acceptor; specific for R-mandelate
Proton acceptor; specific for S-mandelate
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Gly-cisPro motif, important for rejection of L-amino acids
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
Methionine import ATP-binding protein MetN 1
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
ATP-dependent Clp protease ATP-binding subunit ClpX
Resolvase/invertase-type recombinase catalytic
Probable potassium transport system protein Kup
Putative iron-sulfur cluster insertion protein ErpA 1
Dual-specificity RNA methyltransferase RlmN
5-glutamyl coenzyme A thioester intermediate
UDP-N-acetylenolpyruvoylglucosamine reductase
Interaction with polymerase core subunit RpoC
Oxidored_molyb domain-containing protein
Cysteine sulfenic acid (-SOH) intermediate
Ubiquinone biosynthesis O-methyltransferase
Aliphatic sulfonates import ATP-binding protein SsuB 3
Peptide methionine sulfoxide reductase MsrA
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
Glutamate-1-semialdehyde 2,1-aminomutase
Thiol:disulfide interchange protein DsbD
TIGR03759 family integrating conjugative element protein
Ribosomal RNA small subunit methyltransferase A
Lipoprotein-releasing system ATP-binding protein LolD
Sec-independent protein translocase protein TatA
DNA-directed RNA polymerase subunit beta
Gly-zipper_YMGG domain-containing protein
Periplasmic serine endoprotease DegP-like
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Ribosomal RNA small subunit methyltransferase G
Carboxypeptidase regulatory-like domain-containing protein
Periplasmic nitrate reductase, electron transfer subunit
Ribosomal RNA large subunit methyltransferase H
ATP-dependent protease ATPase subunit HslU
Potassium-transporting ATPase KdpC subunit
Ribosomal protein S12 methylthiotransferase RimO
Uncharacterized BphX-like protein Bxeno_C1129
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Cell division topological specificity factor
Cyclic pyranopterin monophosphate synthase
Cytochrome c biogenesis ATP-binding export protein CcmA
Oxygen-dependent coproporphyrinogen-III oxidase
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Iron-sulfur cluster assembly protein CyaY
Uncharacterized HTH-type transcriptional regulator BphR
3-methyl-2-oxobutanoate hydroxymethyltransferase
Phosphoribosylformylglycinamidine cyclo-ligase
Aliphatic sulfonates import ATP-binding protein SsuB 1
Putative pterin-4-alpha-carbinolamine dehydratase
Bifunctional purine biosynthesis protein PurH
Putative iron-sulfur cluster insertion protein ErpA 2
Lipopolysaccharide export system protein LptA
Outer membrane beta-barrel porin/alpha-amylase
ATP-dependent Clp protease proteolytic subunit 2
O-(phosphoribosyl dephospho-coenzyme A)serine
Protein-glutamate methylesterase/protein-glutamine glutaminase
Proton acceptor; for glutaminase activity
Nucleophile; cysteine thiosulfonate intermediate
3-deoxy-manno-octulosonate cytidylyltransferase
Sec-independent protein translocase protein TatB
Large-conductance mechanosensitive channel
ATP-dependent Clp protease proteolytic subunit 1
Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-269)
Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91)
Methionine import ATP-binding protein MetN 2
DNA-directed RNA polymerase subunit beta'
Arabinose import ATP-binding protein AraG
Phosphonates import ATP-binding protein PhnC
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C
1-aminocyclopropane-1-carboxylate deaminase
Exopolysaccharide biosynthesis protein YbjH
ADP-ribosylarginine; by dinitrogenase reductase ADP- ribosyltransferase
ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase
Small ribosomal subunit biogenesis GTPase RsgA
2-dehydro-3-deoxyphosphooctonate aldolase
Twin-arginine translocation pathway signal sequence domain-containing protein
Taurine import ATP-binding protein TauB 3
Putative ribose/galactose/methyl galactoside import ATP-binding protein
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE
TIGR03756 family integrating conjugative element protein
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase
Schiff-base intermediate with substrate; via pyruvic acid
2-fold decrease in catalytic efficiency.
4- to 7-fold decrease in catalytic efficiency with aldehydes three to four carbons in length. Does not significantly reduce the channeling efficiency of the enzyme complex toward acetaldehyde or propanaldehyde.
5- to 16-fold decrease in catalytic efficiency with aldehydes two to four carbons in length. Exhibits 59% of wild-type acetaldehyde channeling efficiency.
5-fold decrease in affinity for acetaldehyde. Increase in affinity for butyraldehyde and pentaldehyde, leading to a 9- and 20-fold increase in catalytic efficiency with butyraldehyde and pentaldehyde as substrate, respectively. Exhibits 84% of wild-type acetaldehyde channeling efficiency.
Displays significant reduction in the level of allosteric activation of the aldol cleavage reaction by BphI.
Does not significantly reduce the channeling efficiency of the enzyme complex toward acetaldehyde or propanaldehyde.
Exhibits preferences for aldehydes similar as wild-type. Exhibits about 80% of wild-type acetaldehyde channeling efficiency. Displays significant reduction in the level of allosteric activation of the aldol cleavage reaction by BphI.
Loss of catalytic activity. Still able to bind NAD(+), however with much lower affinity.
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Formate-dependent phosphoribosylglycinamide formyltransferase
O-(5'-phospho-DNA)-tyrosine intermediate
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
ATP phosphoribosyltransferase regulatory subunit
Isocitrate dehydrogenase kinase/phosphatase
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Outer membrane protein assembly factor BamA
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Aliphatic sulfonates import ATP-binding protein SsuB 2
Biphenyl dioxygenase system ferredoxin subunit
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
DNA-directed RNA polymerase subunit omega
23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
100-fold decrease in the catalytic efficiency of the aldol cleavage reaction. Dramatic reduction in acetaldehyde and propanaldehyde channeling efficiency by more than 70%.
32-fold reduction in the catalytic efficiency with acetaldehyde as substrate of the aldol addition reaction, but no change in the catalytic efficiency using propanaldehyde; thus, exhibits a 40-fold preference for propanaldehyde over acetaldehyde.
4000-fold decrease in the catalytic efficiency of the aldol cleavage reaction.
Able to channel acetaldehyde but not the larger propanaldehyde.
Able to channel butyraldehyde (with less efficiency than wild-type) but not its isomer isobutyraldehyde.
As the wild-type enzyme, exhibits similar catalytic efficiency with acetaldehyde or propanaldehyde as substrate in the aldol addition reaction but displays higher catalytic efficiency with longer aldehydes (50-fold increase using pentaldehyde). Shows a reduction in aldehyde channeling efficiency by 30%.
Displays a reduction in aldehyde channeling efficiency of about 20%.
Loss of aldolase activity (with either enantiomer of HOPA), but retains some decarboxylase activity for the smaller oxaloacetate substrate. In the retro-aldol cleavage reaction, is inactive toward 4(S)-HOPA but is active toward 4(R)-HOPA, albeit with a great reduction in catalytic efficiency, and in the aldol addition reaction, produces also exclusively the 4(R)-enantiomer; when associated with F-290.
Loss of stereochemical control as the mutant is able to catalyze the aldol cleavage of substrates with both R and S configurations at C4 with similar kinetic parameters. 3.5-fold decrease in the catalytic efficiency of the aldol cleavage reaction.
Loss of stereochemical control as the mutant is able to catalyze the aldol cleavage of substrates with both R and S configurations at C4 with similar kinetic parameters. 3.5-fold decrease in the catalytic efficiency of the aldol cleavage reaction. Reduction in aldehyde channeling efficiency by more than 30%. In the retro-aldol cleavage reaction, is inactive toward 4(S)-HOPA but is active toward 4(R)-HOPA, albeit with a great reduction in catalytic efficiency, and in the aldol addition reaction, produces also exclusively the 4(R)-enantiomer; when associated with N-87 or W-87.
Unable to channel either acetaldehyde or propanaldehyde.
tRNA N6-adenosine threonylcarbamoyltransferase
Phosphocysteine intermediate; for EIIB activity
ATP-dependent Clp protease adapter protein ClpS
TIGR03749 family integrating conjugative element protein
UDP-3-O-acylglucosamine N-acyltransferase
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Glutamyl-tRNA(Gln) amidotransferase subunit A
Ribosomal RNA large subunit methyltransferase E
Nucleophile; methyl group acceptor from either O6- methylguanine or O4-methylthymine
Nucleophile; methyl group acceptor from methylphosphotriester
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
Catalyzes the tautomerisation of HOPDA. Extremely low hydrolase activity; when associated with A-265.
Unable to catalyze the tautomerisation of HOPDA. Extremely low hydrolase activity; when associated with A-112.
Biosynthetic peptidoglycan transglycosylase
Putative 3-methyladenine DNA glycosylase
Tripartite tricarboxylate transporter substrate binding protein
Holliday junction ATP-dependent DNA helicase RuvB
D-(-)-3-hydroxybutyrate oligomer hydrolase
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Probable chemoreceptor glutamine deamidase CheD
Heme-containing CO-sensing transcriptional regulator RcoM 1
Loss of ability to bind heme. Loss of ability to respond to CO, presenting constitutive activity.
Loss of ability to bind heme. Loss of activity.
Much higher affinity for CO. Loss of activity. No effect on heme incorporation.
Nitrogenase-stabilizing/protective protein NifW
Deoxyuridine 5'-triphosphate nucleotidohydrolase
3-phosphoshikimate 1-carboxyvinyltransferase
Chromosomal replication initiator protein DnaA
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase
UDP-3-O-acyl-N-acetylglucosamine deacetylase
Small-conductance mechanosensitive channel
Imidazole glycerol phosphate synthase subunit HisF
Leucyl/phenylalanyl-tRNA--protein transferase
2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Pyridoxine/pyridoxamine 5'-phosphate oxidase
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Holliday junction ATP-dependent DNA helicase RuvA
Pyrimidine/purine nucleoside phosphorylase
Enoyl-[acyl-carrier-protein] reductase [NADH]
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
TIGR03757 family integrating conjugative element protein
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
Schiff-base intermediate with acetoacetate
Biphenyl dioxygenase system ferredoxin--NAD(+) reductase component
DNA-directed RNA polymerase subunit alpha
Taurine import ATP-binding protein TauB 1
C4-dicarboxylate ABC transporter substrate-binding protein
NADPH-dependent 7-cyano-7-deazaguanine reductase
Succinate--CoA ligase [ADP-forming] subunit beta