Uncharacterized ABC transporter ATP-binding protein TM_0352
Redox-sensing transcriptional repressor Rex 2
Putative anti-sigma factor antagonist TM_1081
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Protein-L-isoaspartate O-methyltransferase
Uncharacterized ABC transporter ATP-binding protein TM_0288
Holliday junction ATP-dependent DNA helicase RuvA
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
NADPH-dependent 7-cyano-7-deazaguanine reductase
N5-carboxyaminoimidazole ribonucleotide mutase
Ribosomal RNA large subunit methyltransferase H
5-methylthioadenosine/S-adenosylhomocysteine deaminase
Phosphorylated carbohydrates phosphatase TM_1254
Ribosomal protein S12 methylthiotransferase RimO
200-fold decrease of the catalytic efficiency for FGAR. It has no effect on the ATP binding site, however it affects binding of FGAR.
Phosphoribosylformylglycinamidine synthase subunit PurL
Strong decrease of the binding affinity and 20-fold decrease of the catalytic efficiency for FGAR. It has no effect on the ATP binding site, however it affects binding of FGAR.
Phosphate-specific transport system accessory protein PhoU homolog 2
Activity abolished towards fluoroscein diacetoxymethyl ether.
Greater than 10-fold reduction in catalytic efficiency toward the acetate substrates. Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-228.
Minimal change in catalytic efficiency toward acetate substrates.
Minimal change in catalytic efficiency toward the acetate substrates.
Reduces catalytic activity by 33-fold against fluoroscein diacetoxymethyl ether; 8-fold decrease in activity against dimethacryloxymethyl ether; 4-fold increase in catalytic efficiency toward dicyclobutylcarboxymethyl ether. Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-92; A-213 or A-276.
Reduction in catalytic efficiency towards fluoroscein diacetoxymethyl ether; when associated with A-228.
Uncharacterized HTH-type transcriptional regulator TM_0326
Bifunctional shikimate kinase/3-dehydroquinate synthase
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Putative anti-sigma factor antagonist TM_1442
Archaeal-type glutamate synthase [NADPH]
Highly reduced activity. 10-fold reduction of the binding affinity for NAD(+). No change in substrate affinity.
No change in activity and substrate affinity. 5-fold reduction of the binding affinity for NAD(+).
Reduced activity. 300-fold reduction of the binding affinity for NAD(+). No change in substrate affinity.
ATP phosphoribosyltransferase regulatory subunit
O-(5'-phospho-DNA)-tyrosine intermediate
Probable nicotinate-nucleotide adenylyltransferase
Ribose import ATP-binding protein RbsA 1
Phosphate import ATP-binding protein PstB
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Gly-cisPro motif, important for rejection of L-amino acids
3-isopropylmalate dehydratase large subunit 1
Probable glycerol uptake facilitator protein
Holliday junction ATP-dependent DNA helicase RuvB
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
Probable metal transport system ATP-binding protein TM_0124
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Sec translocon accessory complex subunit YajC
Bifunctional purine biosynthesis protein PurH
ATP-dependent protease ATPase subunit HslU
Probable glycine dehydrogenase (decarboxylating) subunit 2
Redox-sensing transcriptional repressor Rex 1
Binds dUMP 670-fold weaker than wild-type.
Involved in ionization of N3 of dUMP, leading to its activation
Still catalytically active although only shows 0.0008% of wild-type activity. Binds dUMP 7300-fold weaker than wild-type.
Still catalytically active although shows a large decrease in activity.
Probable multidrug resistance protein NorM
Energy-coupling factor transporter ATP-binding protein EcfA2
4-hydroxy-tetrahydrodipicolinate reductase
ATP-dependent Clp protease ATP-binding subunit ClpX
Phospho-2-dehydro-3-deoxyheptonate aldolase
Uncharacterized periplasmic metal-binding protein TM_0123
Decreased iron content and loss of activity; when associated with A-150 and A-154.
Decreased iron content and loss of activity; when associated with A-150 and A-157.
Decreased iron content and loss of activity; when associated with A-154 and A-157.
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Loss of activity. Does not prevent binding to mcp2.
Imidazole glycerol phosphate synthase subunit HisF
Chromosomal replication initiator protein DnaA
N-(5'-phosphoribosyl)anthranilate isomerase
UPF0173 metal-dependent hydrolase TM_1162
Carbamoyl-phosphate synthase small chain
Ribose import ATP-binding protein RbsA 2
Energy-coupling factor transporter ATP-binding protein EcfA1
Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
K(+)-stimulated pyrophosphate-energized sodium pump
Silences the K(+)-independent activating Na(+)-binding site.
Protein-glutamate methylesterase/protein-glutamine glutaminase
No appreciable effect on the binding affinity for CheY.
DNA-directed RNA polymerase subunit alpha
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Probable transcriptional regulatory protein TM_0466
Transcription termination/antitermination protein NusG
Phosphoribosylformylglycinamidine synthase subunit PurQ
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Heat-inducible transcription repressor HrcA
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Release factor glutamine methyltransferase
Polyribonucleotide nucleotidyltransferase
Proton acceptor; for glutaminase activity
N-acetyl-gamma-glutamyl-phosphate reductase
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Glyceraldehyde-3-phosphate dehydrogenase
Interaction with polymerase core subunit RpoC
Loss of activity; when associated with R-219.
No change in activity. Retains weak activity; when associated with F-21. Loss of activity; when associated with F-107.
Retains weak activity; when associated with R-219.
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Phosphate-specific transport system accessory protein PhoU homolog 1
Energy-coupling factor transporter transmembrane protein EcfT
Putative 1-aminocyclopropane-1-carboxylate deaminase
Flagellar hook-basal body complex protein FliE
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Bifunctional thiamine biosynthesis protein ThiDN
Abolishes pentamerization. Abolishes ion transport.
Abolishes pentamerization. Mildly decreases ion transport.
Abolishes pentamerization. Strongly decreases ion transport.
Impairs pentamerization. Decreases ion transport.
Increases ion transport by suppression of a kink in the transmembrane ion permeation pathway.
Increases ion transport by suppression of an obstruction in the transmembrane ion permeation pathway.
Increases protein stability. Decreases ion transport.
No effect on pentamerization. Abolishes ion transport.
No effect on pentamerization. Decreases ion transport.
No effect on pentamerization. Increases ion transport.
No effect on pentamerization. No effect on ion transport.
No significant decrease of Co(2+) uptake, but abolishes selectivity for Co(2+).
Reduces Co(2+) uptake. Abolishes Co(2+) uptake; when associated with L-295.
Strongly reduces Co(2+) uptake. Abolishes Co(2+) uptake; when associated with L-299.
Probable glycine dehydrogenase (decarboxylating) subunit 1
Part of the EHPQR-motif; important for interaction with FliM
tRNA N6-adenosine threonylcarbamoyltransferase
Imidazole glycerol phosphate synthase subunit HisH
DNA-directed RNA polymerase subunit beta'
ATP-dependent Clp protease proteolytic subunit
Probable septum site-determining protein MinC
Schiff-base intermediate with substrate; via pyruvic acid
Phenylalanine--tRNA ligase alpha subunit
Putative dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Has little effects on the NTPase activity.
Nucleoside triphosphate pyrophosphohydrolase/pyrophosphatase MazG
Reduces the NTPase activity to 10% of the wild-type activity.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with A-97.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with A-98.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with Q-41.
Reduces the NTPase activity to 10% of the wild-type activity; when associated with Q-42.
Probable 2-phosphosulfolactate phosphatase
Carbamoyl-phosphate synthase large chain
Forms about 11 Angstroms pore, nanocompartments appear normal. Increased metabolic flux across the pore.
Forms about 5 Angstroms pore, nanocompartments appear normal.
Forms about 6 Angstroms pore, nanocompartments appear normal.
Loss of flavin-binding. No change in iron storage or iron release under aerobic or anaerobic conditions.
Still makes about 3 Angstroms pore, nanocompartments appear normal.
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Cleaves more rapidly than the wild-type.
Cleaves much more slowly than the wild-type, but the addition of hydroxylamine which is known to cleave ester bonds leads to the cleavage of this mutant.
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
Ribosomal RNA small subunit methyltransferase H
3-isopropylmalate dehydratase small subunit 2
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
Probable dual-specificity RNA methyltransferase RlmN
Able to regulate the E.coli S10 operon, but does not improve association with E.coli ribosome; when associated with E141Q, Q144K, Q147A, S149E, K151delta, K152delta, I155M, P158G, W159E and K160L.
Considerably improves stable incorporation into E.coli ribosomes, however no change in regulation of the E.coli S10 operon.
Little change in either regulation of the E.coli S10 operon or ribosome assembly.
Transcription antitermination protein NusB
Sugar fermentation stimulation protein homolog
Significant decrease of the kinase activity with a 17-fold decrease of catalytic efficiency.
Significant decrease of the kinase activity with a 180-fold decrease of catalytic efficiency.
200-fold decrease in catalytic efficiency. Loss of activity; when associated with A-422.
300-fold decrease in catalytic efficiency.
300-fold decrease in catalytic efficiency. Loss of activity; when associated with A-310.
Glutamyl-tRNA(Gln) amidotransferase subunit C
Pyridoxal 5'-phosphate synthase subunit PdxT
DNA-directed RNA polymerase subunit beta
Schiff-base intermediate with acetaldehyde
Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-67.
Eliminates binding of one iron-sulfur cluster; when associated with A-63 and A-70.
Eliminates binding of one iron-sulfur cluster; when associated with A-67 and A-70.
[FeFe] hydrogenase maturase subunit HydE
Complete loss of protease activity and of oligomerization.
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase
3-oxoacyl-[acyl-carrier-protein] reductase FabG
Small ribosomal subunit biogenesis GTPase RsgA
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Putative UDP-N-acetylglucosamine 2-epimerase
Probable branched-chain-amino-acid aminotransferase
tRNA-5-methyluridine(54) 2-sulfurtransferase
1500-fold lower catalytic rate and 1000-fold lower catalytic efficiency than the wild-type enzyme with p-nitrophenyl-alpha-D-galactopyranoside as substrate.
Between 200 and 800-fold lower catalytic rate and between 300 and 1700-fold lower catalytic efficiency than the wild-type enzyme with aryl-alpha-galactosides as substrates.
Increased transglycosylating activity at high concentrations of p-nitrophenyl-alpha-D-galactopyranoside substrate, which could be useful in industry and medicine for the synthesis of different p-nitrophenyl-digalactosides.
Increased transglycosylating activity at high concentrations of p-nitrophenyl-alpha-D-galactopyranoside substrate, which could be useful in industry and medicine for the synthesis of different p-nitrophenyl-digalactosides. Able to produce 16 times more of a regio-isomer with the (alpha1,2)-bond than wild-type enzyme.
Less than 1% of the wild-type enzyme activity with p-nitrophenyl-alpha-D-galactopyranoside as substrate at 80 degrees Celsius.
Reduced activity compared to the wild-type enzyme.
Cysteine sulfenic acid (-SOH) intermediate
Interchain (with Cys-211); in linked form
Interchain (with Cys-48); in linked form
Phosphoribosylformylglycinamidine synthase subunit PurS
Probable DNA double-strand break repair Rad50 ATPase
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--LD-lysine ligase
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-112. In presence of CheD, reduced activity but exceeds activity of CheC alone; when associated with S-112.
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-115. In presence of CheD, almost no activity; when associated with S-115.
Loss of activity, in absence of CheD; in presence of CheD, activity greater than wild-type CheC alone. Loss of activity, in absence of CheD; when associated with S-13. In presence of CheD, reduced activity but exceeds activity of CheC alone; when associated with S-13.
Loss of activity, in absence of CheD; in presence of CheD, reduced activity. Loss of activity, in absence of CheD; when associated with S-16. In presence of CheD, almost no activity; when associated with S-16.
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Ribosomal RNA small subunit methyltransferase I
GDP-mannose:di-myo-inositol-1,3'-phosphate beta-1,2-mannosyltransferase
4-hydroxy-tetrahydrodipicolinate synthase
Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.
Galactose-1-phosphate uridylyltransferase
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
3-isopropylmalate dehydratase large subunit 2
Uncharacterized RNA methyltransferase TM_1094
DNA-directed RNA polymerase subunit omega
3-methyl-2-oxobutanoate hydroxymethyltransferase
Putative membrane protein insertion efficiency factor
3-isopropylmalate dehydratase small subunit 1
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Spermidine/putrescine import ATP-binding protein PotA
No effect on DNA binding. No effect on cleavage of DNA containing inosine. Strongly reduced cleavage at apurinic sites.
No effect on cleavage of DNA containing inosine.
Reduced affinity for DNA. No effect on cleavage of DNA containing inosine. Abolishes cleavage at apurinic sites.
Strongly reduced cleavage of DNA containing inosine.
Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein