SPbeta prophage-derived uncharacterized protein YoqC
Putative N-acetylmuramoyl-L-alanine amidase YrvJ
Uncharacterized HTH-type transcriptional regulator YobS
Melibiose/raffinose/stachyose-binding protein MelE
Probable multidrug resistance protein YpnP
Uncharacterized ABC transporter ATP-binding protein YhcG
Branched-chain-amino-acid aminotransferase 2
Probable ATP-dependent RNA helicase YfmL
L-glutamine--4-(methylsulfanyl)-2-oxobutanoate aminotransferase
Probable flagellar assembly protein FliH
Sulfite reductase [NADPH] hemoprotein beta-component
N-acetylglucosamine-6-phosphate deacetylase
Manganese transport system ATP-binding protein MntB
Not toxic in E.coli, 100-fold decreased RNA-binding.
Not toxic in E.coli, 50-fold decreased RNA-binding.
Not toxic in E.coli, 625-fold decreased RNA-binding.
Not toxic in E.coli, 70-fold decreased RNA-binding.
tRNA N6-adenosine threonylcarbamoyltransferase
Holliday junction ATP-dependent DNA helicase RuvB
Contains heme B and heme A at levels comparable to that of wild-type CtaA.
Contains small amounts of heme B and heme A.
Decrease in heme A synthase activity and 5-fold reduction in cytochrome c oxidase activity. Contains heme B, heme O, and heme I, as well as trace amounts of heme A.
Inactive. Contains heme B and heme O, with mainly heme O bound to CtaA.
scyllo-inositol 2-dehydrogenase (NADP(+)) IolW
Putative ABC transporter substrate-binding lipoprotein YhfQ
In absence of the other RsbR paralogs decreased stress response.
In absence of the other RsbR paralogs no change in stress response.
In absence of the other RsbR paralogs small decrease in stress response.
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Bis(5'-nucleosyl)-tetraphosphatase PrpE [asymmetrical]
Signal recognition particle receptor FtsY
Probable ribosome maturation protein RlbA
Probable glycine dehydrogenase (decarboxylating) subunit 2
Confers streptomycin resistance and very poor growth in rich medium.
In RPSL1 (KO-267); confers streptomycin resistance but not hyperaccurate translation, increases natural antibiotic production 10-fold.
In RPSL4 (STRA1); confers streptomycin resistance and hyperaccurate translation, has no effect on natural antibiotic production.
Lack of phosphorylation. No chemotaxis, tumbly swimming behavior and shows exclusively clockwise rotation.
No chemotaxis, tumbly swimming behavior and shows exclusively clockwise rotation.
Probable coenzyme A transferase subunit beta
Retains NADPH-dependent oxidase activity under aerobic conditions, but at a lower level. No effect on S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-122 or with A-122 and A-220. Loss of bacilliredoxin reductase activity; when associated with A-122 and A-220.
Retains NADPH-dependent oxidase activity under aerobic conditions. Loss of S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-14 or with A-14 and A-122. Loss of bacilliredoxin reductase activity; when associated with A-14 and A-122.
Retains NADPH-dependent oxidase activity under aerobic conditions. No effect on S-bacillithiolation. Decreased bacilliredoxin reductase activity. Decreased NADPH-dependent oxidase activity under aerobic conditions; when associated with A-14 or with A-14 and A-220. Loss of bacilliredoxin reductase activity; when associated with A-14 and A-220.
50S ribosomal subunit assembly factor BipA
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YopO
Uncharacterized ABC transporter ATP-binding protein YurJ
Dihydrofolate synthase/folylpolyglutamate synthase
SPbeta prophage-derived sublancin-168-processing and transport ATP-binding protein SunT
Tryptophan RNA-binding attenuator protein inhibitory protein
Probable protein-export membrane protein SecG
Phosphocysteine intermediate; for EIIB activity
Putative PTS system EIIBC component YbbF
Sec-independent protein translocase protein TatAc
Putative 6-phosphogluconate dehydrogenase YqeC
Uncharacterized MFS-type transporter YvqJ
DEAD-box ATP-dependent RNA helicase CshA
Peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK
Glycerol phosphate lipoteichoic acid synthase 1
Processed glycerol phosphate lipoteichoic acid synthase 1
ATP-dependent helicase/nuclease subunit A
Loss of helicase and nuclease activity. DNA-binding is unaltered.
Some loss of nuclease activity, helicase and DNA-binding are unaltered. No production of chi fragments; when associated with A-961 in AddB nearly complete loss of nuclease activity.
6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating
SPbeta prophage-derived uncharacterized protein YotM
Levansucrase and sucrase synthesis operon antiterminator
Uncharacterized glycosyltransferase YfnE
Cell division topological determinant MinJ
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
SPbeta prophage-derived uncharacterized protein YopE
In DnaBI; required for chromosome and plasmid replication.
In DnaBII; required only for chromosome replication.
Replication initiation and membrane attachment protein
Sigma-K factor-processing regulatory protein BofA
Uncharacterized ABC transporter permease protein YtlD
Uncharacterized N-acetyltransferase YuaI
Ribosomal large subunit pseudouridine synthase B
SPbeta prophage-derived uncharacterized protein YotC
Choline transport ATP-binding protein OpuBA
Sec-independent protein translocase protein TatAd
Uncharacterized UDP-glucosyltransferase YojK
Loss of activity. Decreases iron binding. Cannot complement the deletion mutant. Lacks activity and does not binds iron; when associated with S-318.
Loss of activity. Does not bind iron. Cannot complement the deletion mutant.
No change in activity and in iron content.
No change in activity and in iron content. Cannot complement the deletion mutant. Lacks activity and does not binds iron; when associated with S-320.
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YonR
Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-7.
Complete loss of protease activity. The mutant is only found as a monomer; when associated with A-8.
Leads to a 13-fold reduction of CO(2) production.
Leads to a 20-fold reduction of CO(2) production.
Leads to a 25-fold reduction of activity and a 4-fold reduction of CO(2) production.
Cannot restore a sporulation-proficient phenotype in a phrA deletion mutant.
Restores a sporulation-proficient phenotype in a phrA deletion mutant.
Uncharacterized zinc-type alcohol dehydrogenase-like protein YjmD
PTS system N-acetylglucosamine-specific EIICB component
Peptide methionine sulfoxide reductase MsrB
Bifunctional non-homologous end joining protein LigD
Putative metal-sulfur cluster biosynthesis proteins YuaD
Uncharacterized HTH-type transcriptional regulator YhgD
SPbeta prophage-derived uncharacterized protein YomX
Iron(3+)-hydroxamate-binding protein YxeB
Putative cytochrome bd menaquinol oxidase subunit II
Uncharacterized MFS-type transporter YfkF
10-fold decrease in catalytic efficiency with ABTS as substrate. 91-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
165-fold decrease in catalytic efficiency with ABTS as substrate. 400-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
25-fold decrease in catalytic efficiency with ABTS as substrate. 30-fold decrease in catalytic efficiency with SGZ as substrate.
33-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
357-fold decrease in catalytic efficiency with ABTS as substrate. 152-fold decrease in catalytic efficiency with SGZ as substrate.
5-fold decrease in catalytic efficiency with ABTS as substrate. 785-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
6-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
9-fold decrease in catalytic efficiency with ABTS as substrate. 26-fold decrease in catalytic efficiency with 2,6-DMP as substrate.
9-fold decrease in catalytic efficiency with ABTS as substrate. Almost loss of activity with 2,6-DMP as substrate.
Decreases copper content. Strong decrease in catalytic efficiency with both ABTS and SGZ.
Does not affect copper content. Strong decrease in catalytic efficiency with both ABTS and SGZ.
Loss of laccase activity. Mutant fails to develop the dark brown phenotype typical of the wild type strain.
Loss of laccase activity. Mutant fails to develop the dark brown phenotype typical of the wild type strain. Decreases copper content.
Retains approximately 20% of the wild-type activity with both ABTS and SGZ.
Retains approximately 30% of the wild-type activity with SGZ but does not affect activity with ABTS.
Retains approximately 50% of the wild-type activity with both ABTS and SGZ.
Retains approximately 60% of the wild-type activity with both ABTS and SGZ.
Retains approximately 70% of the wild-type activity with both ABTS and SGZ.
Slight decrease in catalytic efficiency. Shows minimal changes in the structure of the copper centers.
Strong decrease in catalytic efficiency. Significant differences in both the type 1 and type 2 copper centers.
Antilisterial bacteriocin subtilosin biosynthesis protein AlbG
Polyketide biosynthesis acyltransferase homolog PksD
Galactooligosaccharides transport system permease protein GanP
Nucleophile; for GTP cyclohydrolase activity
Proton acceptor; for GTP cyclohydrolase activity
Glycine betaine/carnitine/choline transport ATP-binding protein OpuCA
Putative enoyl-CoA hydratase/isomerase YngF
Probable metabolite transport protein CsbC
SPbeta prophage-derived uncharacterized protein YonE
Drastic decrease in arabinanase activity. Does not unduly disrupt the calcium cluster. Mutation does not disrupt the calcium cluster, but it relaxes the geometry of the cluster, resulting in a more planar arrangement of the calcium ion with five of the water molecules.
Extracellular endo-alpha-(1->5)-L-arabinanase 2
Putative sigma L-dependent transcriptional regulator YplP
Severely reduces ATPase activity and growth rate.
tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Sporulation thiol-disulfide oxidoreductase A
Putative thiamine pyrophosphate-containing protein YdaP
Protein-glutamine gamma-glutamyltransferase
SPbeta prophage-derived uncharacterized protein YomN
Uncharacterized RNA methyltransferase YfjO
Fe(3+)-citrate import system permease protein YfmD
Putative 3-methyladenine DNA glycosylase
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Cells no longer transformable but still undergo competence-associated growth arrest; protein localizes to the cell pole.
Uncharacterized AAA domain-containing protein YrvN
Uncharacterized N-acetyltransferase YkwB
SPbeta prophage-derived uncharacterized protein YosJ
Uncharacterized N-acetyltransferase CgeE
Arabinose metabolism transcriptional repressor
SPbeta prophage-derived uncharacterized protein YomE
L-cystine import ATP-binding protein TcyN
Phosphoribosylformylglycinamidine cyclo-ligase
Peptidoglycan beta-N-acetylmuramidase NamZ
D-alanyl-D-alanine carboxypeptidase DacA
serine-type D-Ala-D-Ala carboxypeptidase
ADP-dependent (S)-NAD(P)H-hydrate dehydratase
Processive diacylglycerol beta-glucosyltransferase
SPbeta prophage-derived uncharacterized protein YokH
NADPH-dependent 7-cyano-7-deazaguanine reductase
Uncharacterized HTH-type transcriptional regulator YxaD
Uncharacterized ABC transporter permease YclI
2-iminobutanoate/2-iminopropanoate deaminase
Complete loss of growth without mevalonate.
Does not activate SufS; dominant negative to wild-type protein, interacts with SufS. Binds about 40% Zn(2+).
Does not interact with SufS, does not activate SufS; no effect in presence of wild-type protein. Binds about 15% Zn(2+).
Does not interact with SufS, does not activate SufS; no effect in presence of wild-type protein. Binds about 45% Zn(2+).
Increases stability of the bound Fe-S cluster. Binds SufS, binds about 35% Zn(2+).
Uncharacterized N-acetyltransferase YitH
Teichoic acids export ATP-binding protein TagH
SPbeta prophage-derived uncharacterized protein YorZ
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
Loss of debacillithiolation of S-bacillithiolated Bdr (Bdr-SSB).
Sporulation-specific transcription factor SpoVIF
Uncharacterized sodium-dependent transporter YocR
In divIVA1; loss of function resulting in sporulation defect and in the formation of minicells.
Localization at cytoplasmic foci instead of the cell pole. Stable interaction with Spo0J and association with the chromosome.
No change in membrane fusion. Still binds GTP, no GTPase activity. No longer binds GTP, no GTPase activity; when associated with A-56. Double mutant causes better vesicle and content mixing than wild-type.
No change in membrane fusion. Still binds GTP, no GTPase activity. No longer binds GTP, no GTPase activity; when associated with A-625. Double mutant causes better vesicle and content mixing than wild-type.
Peptidoglycan-N-acetylmuramic acid deacetylase PdaC
Uncharacterized MFS-type transporter YcnB
SPbeta prophage-derived uncharacterized protein YotF
SPbeta prophage-derived uncharacterized protein YonU
Uncharacterized amino acid permease YecA
No longer prevents EndoA toxicity upon coexpression in E.coli, due to loss of EndoA-EndoAI interaction.
Still prevents EndoA toxicity upon coexpression in E.coli.
Uncharacterized N-acetyltransferase YhdJ
3-isopropylmalate dehydratase small subunit
Uncharacterized RNA pseudouridine synthase YlyB
Ribosomal RNA small subunit methyltransferase H
Uncharacterized UDP-glucosyltransferase YdhE
Uncharacterized cysteine-rich protein YhjQ
Can restore growth of E.coli secA mutants.
Forms position 587-750 dimers upon oxidation in vitro; when associated with C-587. Also forms position 750-750 dimers (homodimers).
Forms position 587-750 dimers upon oxidation in vitro; when associated with C-750. Does not form position 587-587 dimers (homodimers).
Forms position 588-588 dimers upon oxidation in vitro (homodimers).
Loss of activity. Cannot complement E.coli secA mutants.
Gluconate operon transcriptional repressor
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
In sapB2 and sapB10; activates alkaline phosphatase during sporulation.
Methionine import system permease protein MetP
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
SPbeta prophage-derived uncharacterized protein YorP
Glycine betaine transport ATP-binding protein OpuAA
PTS system oligo-beta-mannoside-specific EIIA component
Transcription termination/antitermination protein NusA
SPbeta prophage-derived putative transcriptional regulator YosT
Lichenan-specific phosphotransferase enzyme IIB component
SPbeta prophage-derived uncharacterized protein YonH
Probable 3-hydroxyacyl-CoA dehydrogenase
Maltodextrin transport system permease protein MdxG
Arginine biosynthesis bifunctional protein ArgJ alpha chain
Arginine biosynthesis bifunctional protein ArgJ beta chain
Putative phosphotransferase enzyme IIA component YpqE
Putative fluoride ion transporter CrcB 1
Abolishes cytochrome c oxidase assembly.
The measured mass is closest to that of the mature protein with 2 palmitate moieties.
Probable spore germination protein GerPC
Probable peptide export ATP-binding protein YydI
Hypoxanthine-guanine phosphoribosyltransferase
Methyl-accepting chemotaxis protein TlpB
Resolvase/invertase-type recombinase catalytic
SPbeta prophage-derived uncharacterized protein YorF
Uncharacterized HTH-type transcriptional regulator YcgE
Completely abolishes the tyrosine-phosphatase activity.
Low molecular weight protein-tyrosine-phosphatase YfkJ
Antilisterial bacteriocin subtilosin biosynthesis protein AlbD
Energy-coupling factor transporter transmembrane protein EcfT
The protein does not accumulate and cells grow very slowly.
Putative [ribosomal protein S18]-alanine N-acetyltransferase
SPbeta prophage-derived uncharacterized protein YosH
Spore germination protein-like protein YpzD
Retains its autophosphorylation activity, but cannot phosphorylate DegU.
Retains its autophosphorylation activity, but shows a decrease in DegU phosphorylation activity. Decreases the rate of dephosphorylation of DegU.
Shows increased phosphorylation, as well as increased autophosphorylation and phosphotransfer to DegU. Negatively affects competence development.
Signal transduction histidine-protein kinase/phosphatase DegS
Uncharacterized HTH-type transcriptional regulator YugG
O-(3'-phospho-DNA)-tyrosine intermediate
Probable polysaccharide deacetylase PdaB
ABC transporter guanosine-binding protein NupN
Probable membrane transporter protein YjnA
SPbeta prophage-derived uncharacterized protein YorX
Greater than wild-type diphosphate release.
Uncharacterized zinc-type alcohol dehydrogenase-like protein YycR
Glutamate-1-semialdehyde 2,1-aminomutase
Uncharacterized NAD(P)H oxidoreductase YrkL
O-(5'-phospho-DNA)-tyrosine intermediate
SPbeta prophage-derived DNA ligase-like protein LigB
Bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
Is severely impaired in adaptation to the addition of asparagine. Shows near normal counterclockwise bias in the absence of attractant. Is not phosphorylated in the presence of CheA-P.
SPbeta prophage-derived disulfide bond formation protein A
Aliphatic sulfonates import ATP-binding protein SsuB
Uncharacterized HTH-type transcriptional regulator YwbI
Uroporphyrinogen-III C-methyltransferase
Ribonucleoside-diphosphate reductase subunit beta
Uncharacterized isochorismatase family protein PncA
4-fold reduction in activity. Abolished activity; when associated with A-162; A-163 and A-165.
4-fold reduction in activity. Abolished activity; when associated with A-162; A-163 and A-167.
4-fold reduction in activity. Abolished activity; when associated with A-162; A-165 and A-167.
4-fold reduction in activity. Abolished activity; when associated with A-163; A-165 and A-167.
Abolishes autophosphorylation and decreases spore production and biofilm formation. No phosphorylation of YezB.
Does not support germination in response to peptidoglycan.
Assimilatory nitrate reductase electron transfer subunit
Probable xanthine dehydrogenase subunit A
ATP-dependent Clp protease ATP-binding subunit ClpX
Uncharacterized glycosyltransferase YqgM
Abolished D-alanylation of lipoteichoic acid (LTA).
Does not affect D-alanylation of lipoteichoic acid (LTA).
Uncharacterized transcriptional regulatory protein YesN
Multidrug-efflux transporter 2 regulator
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Phosphoribosylformylglycinamidine synthase subunit PurQ
Melibiose/raffinose/stachyose import permease protein MelC
Putative major teichoic acid biosynthesis protein C
Almost abolishes the glycosyltransferase activity.
Drastic loss of activity for both pterostilbene glycosylation and UDP glycosylation.
Enhances the catalytic efficiency of UDP glycosylation, but reduces the relative activity of pterostilbene glycosylation reaction.
Increases UDP glycosylation efficiency and enhances pterostilbene glycosylation activity.
Increases pterostilbene glycosylation activity and UDP glycosylation efficiency.
Leads to about 2-fold decrease in specific activities.
No change in activity. Dramatic reduction of the catalytic efficiency and glycosylation levels; when associated with A-128.
No change in activity. Dramatic reduction of the catalytic efficiency and glycosylation levels; when associated with A-129.
Significant decrease in activity for both pterostilbene glycosylation and UDP glycosylation.
Significant reduction in catalytic efficiency and glycosylation levels.
Significantly enhances the UDP glycosylation efficiency, but shows unchanged or reduced pterostilbene glycosylation when supplied with UDP-glucose and ADP-glucose, respectively.
Strong decrease in activity for both pterostilbene glycosylation and UDP glycosylation.
Spore germination protein-like protein YueG
Galactooligosaccharides transport system permease protein GanQ
SPbeta prophage-derived uncharacterized protein YokK
Glutamyl-tRNA(Gln) amidotransferase subunit C
Probable peptidoglycan endopeptidase LytE
PTS system trehalose-specific EIIBC component
Type II restriction enzyme BsuMI component YdiS
No activity. No iron-sulfur cluster formed.
Performs first half of the reaction. No effect on iron-sulfur cluster formation.
In TS-21; temperature-sensitive, develops, at the nonpermissive temperature (47 degrees Celsius), a coccoid-like morphology, i.e. a phenotype associated with a deficient synthesis of poly(glycerol phosphate) teichoic acid.
Marked diminution of methanol upon both addition and removal of asparagine. No release of methanol upon asparagine addition but methanol release upon asparagine removal is not affected; when associated with D-630. Release of methanol upon both asparagine addition and removal; when associated with D-637. No methylation; when associated with D-630 and D-637.
Marked diminution of methanol upon both addition and removal of asparagine; fails to adapt to addition of asparagine. Release of methanol upon both asparagine addition and removal; when associated with D-371. Releases methanol upon asparagine addition but not upon asparagine removal; when associated with D-630. No methylation; when associated with D-371 and D-630.
Marked diminution of methanol upon both addition and removal of asparagine; methanol release delayed by about 1 minute compared to wild-type; adapts normally to addition of asparagine but fails to adapt to asparagine removal. No release of methanol upon asparagine addition but methanol release upon asparagine removal is not affected; when associated with D-371. Releases methanol upon asparagine addition but not upon asparagine removal; when associated with D-637. No methylation; when associated with D-371 and D-637.
Methyl-accepting chemotaxis protein McpB
Putative PTS system glucosamine-specific EIICBA component
Tele-phosphohistidine intermediate; for EIIA activity
4-hydroxy-tetrahydrodipicolinate reductase
Cysteine sulfenic acid (-SOH) intermediate
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
Probable siderophore transport system ATP-binding protein YusV
SPbeta prophage-derived uncharacterized protein YorI
Polyketide biosynthesis malonyl CoA-acyl carrier protein transacylase PksC
Loss of activity. Affects the oligomeric state.
UDP-N-acetylbacillosamine N-acetyltransferase
Uncharacterized HTH-type transcriptional regulator YozG
Does not abrogate the development of aerial mycelium in S.griseus.
Probable quorum-quenching lactonase YtnP
Phosphate import ATP-binding protein PstB 2
Petrobactin import system permease protein YclO
In sow-10; suppresses the motility loss and flagellar assembly defect of an fliW deletion.
In sow-11; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-12; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-15; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-2 and sow28; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
In sow-20; suppresses the motility loss and flagellar assembly defect of an fliW deletion.
In sow-8 and sow-4; suppresses the motility loss and flagellar assembly defect of an fliW deletion. Binds FliW.
Inhibits motility and flagellar filament assembly, greatly reduced binding of FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW.
Inhibits motility and flagellar filament assembly, reduced binding of FliW.
Inhibits motility and flagellar filament assembly, still binds FliW. Binds 5'-UTR of hag mRNA, is not completed by FliW.
SPbeta prophage-derived uncharacterized protein YonA
Transition state regulatory protein AbrB
Uncharacterized FAD-linked oxidoreductase YitY
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit A
Uncharacterized MFS-type transporter YkuC
Putative HMP/thiamine import ATP-binding protein YkoD
Aminodeoxychorismate/anthranilate synthase component 2
Uncharacterized ABC transporter ATP-binding protein YknU
Probable inorganic carbon transporter subunit DabB
Nicotinate-nucleotide adenylyltransferase
SPbeta prophage-derived uncharacterized protein YoqY
Schiff-base intermediate with KHG or pyruvate
L-threonine dehydratase biosynthetic IlvA
Succinate-semialdehyde dehydrogenase [NADP(+)]
Uncharacterized metallophosphoesterase-like protein YydB
SPbeta prophage-derived uncharacterized membrane protein YosW
Sigma-G-dependent sporulation-specific SASP protein
Putative propionyl-CoA carboxylase beta chain
Probable low-affinity inorganic phosphate transporter
Coenzyme A biosynthesis bifunctional protein CoaBC
Cleaves Holliday junctions, no interaction with RecA, greatly increased sensitivity to DNA-damaging agents.
Greatly increased sensitivity to DNA-damaging agents, chromosome segregation defects. Binds DNA and is able to cleave a Holliday junction.
Greatly increased sensitivity to DNA-damaging agents, chromosome segregation defects. Binds DNA but cannot cleave a Holliday junction.
Loss of Holliday junction resolution activity.
Reduces Holliday junction resolution activity 6-fold.
Uncharacterized HTH-type transcriptional regulator YkuM
Acyl-ester intermediate; for transpeptidase activity
Proton donor; for transglycosylase activity
Probable HTH-type transcriptional repressor ExuR
SPbeta prophage-derived membrane protein YosA
Cytochrome bd ubiquinol oxidase subunit 1
Probable iron-sulfur-binding oxidoreductase FadF
Destabilizes MgsR approximately by half. Almost loss of activity.
Destabilizes MgsR approximately by half. No change in activity.
Destabilizes MgsR approximately by half. Strong decrease in activity.
Loss of activity. Strongly diminishes expression of mgsR and of the MgsR regulon.
Slight increase in MgsR stability. Strong decrease in activity.
Strong decrease in MgsR stability. Almost loss of activity.
Strong decrease in MgsR stability. Strong decrease in activity.
Probable UbiX-like flavin prenyltransferase
Oligopeptide transport ATP-binding protein AppF
Putative bacteriocin-SkfA transport system permease protein SkfF
Flagellar hook-basal body complex protein FlhP
Uncharacterized HTH-type transcriptional regulator YdeL
Uncharacterized ABC transporter ATP-binding protein YvrA
Uncharacterized HTH-type transcriptional regulator YyaN
SPbeta prophage-derived uncharacterized protein YoqN
Uncharacterized HTH-type transcriptional regulator YvdT
Electron transfer flavoprotein subunit beta
Polygalacturonan/rhamnogalacturonan-binding protein YtcQ
Uncharacterized HTH-type transcriptional regulator YisV
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Teichuronic acid biosynthesis protein TuaE
SPbeta prophage-derived uncharacterized protein YosK
RDXXRA motif of the pArg binding pocket involved in allosteric regulation
Stabilizes quantity of CtsR to an extent a little lower to that observed for the mcsB deletion, indicating a decrease of protein phosphorylation activity of McsB in vivo.
Stabilizes quantity of CtsR to an extent comparable to that observed for the mcsB deletion, indicating a loss of protein phosphorylation activity of McsB in vivo.
Guanosine import ATP-binding protein NupO
Probable multidrug resistance protein NorM
Uncharacterized MFS-type transporter YbfB
Uncharacterized isochorismatase family protein YwoC
Putative arsenical pump membrane protein YdfA
Multidrug-efflux transporter 1 regulator
Dipeptide transport system permease protein DppC
Schiff-base intermediate with substrate; via pyruvic acid
Prophage-derived-like uncharacterized protein YozM
SPbeta prophage-derived uncharacterized protein YorQ
Has little or no effect on sporulation in wild-type SpoIIP cells. Restores sporulation efficiency of SpoIIP G-108 mutant almost to the level of the wild-type. Localization of SpoIIP G-108 mutant to the septum is about 78% that of wild-type SpoIIP. Fails to suppress the sporulation defect of mutants SpoIIP R-189 and R-278 as well as SpoIIP double mutant L-54 and P-114.
Partially restores the sporulation efficiency of SpoIIP G-108 mutant.
Putative 2-hydroxyacid dehydrogenase YoaD
Minor teichoic acid biosynthesis protein GgaB
2-fold reduced cleavage of SigE I-17 mutant.
60% reduced cleavage of SigE I-17 mutant.
70% reduced cleavage of SigE I-17 mutant.
75% reduced cleavage of SigE I-17 mutant.
80% reduced cleavage of SigE I-17 mutant.
80% reduced cleavage of SigE I-17 mutant. Abolishes cleavage of SigE I-17 mutant; when associated with G-296.
Abolishes cleavage of SigE I-17 mutant; when associated with N-298.
Abolishes cleavage of wild-type SigE. Abolishes cleavage of SigE I-17 mutant.
Nearly unaffected cleavage of SigE I-17 mutant.
Sporulation sigma-E factor-processing peptidase
Unaffected cleavage of SigE I-17 mutant.
Unaffected cleavage of wild-type and K-25 mutant SigE in sporulating B.subtilis cells (PubMed:8002606). Abolishes cleavage of wild-type and K-25 mutant SigE when proteins are coexpressed in E.coli cells (PubMed:21362630).
Beta-ketoacyl-[acyl-carrier-protein] synthase III 2
No effect on protease activity in vitro.
Uncharacterized ABC transporter ATP-binding protein YclH
Uncharacterized MFS-type transporter YjbB
Uncharacterized ABC transporter permease YvrN
Important for interaction with partner proteins
Putative enoyl-CoA hydratase/isomerase YhaR
Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha
Uncharacterized MFS-type transporter YitG
Catalytic mutant, no alteration in rRNA binding.
Retains nuclease activity, still stimulated by L3.
Impairs cleavage of the yitJ riboswitch.
Increased protein expression (replaces with transmembrane helix from EzrA).
20-fold reduction in sporulation efficiency. Largely enriched at the forespore periphery, although some localization to the cytosol. Binding of ATP reduced over 23-fold. Leads to diminished ATP hydrolysis.
500-fold decrease in sporulation efficiency. 5-log decrease in sporulation efficiency; no ATPase activity, but no change in ATP-binding ability; unable to polymerize and fails to promote coat assembly; when associated with A-71.
Altered cortex and coat formation, affects localization to the forespore.
Altered cortex and coat formation, affects localization to the forespore; when associated with P-400 and R-457.
Altered cortex and coat formation, affects localization to the forespore; when associated with P-400 and V-418.
Altered cortex and coat formation, affects localization to the forespore; when associated with R-457 and V-418.
Altered cortex and coat formation. Possesses swirls of coat in the mother cell cytoplasm and does not possess a cortex.
Altered cortex and coat formation; when associated with L-69.
Altered cortex and coat formation; when associated with S-230.
Altered cortex and coat formation; when associated with V-367 and R-383.
Completely abolished sporulation efficiency. No ATPase activity, but no change in ATP-binding ability. Unable to polymerize and fails to promote coat assembly;.
Defective in germination; when associated with A-210 and R-383. Altered cortex and coat formation; when associated with M-210 and R-383.
Defective in germination; when associated with A-210 and V-367. Altered cortex and coat formation; when associated with M-210 and R-383.
Defective in germination; when associated with N-283.
Defective in germination; when associated with P-59.
Defective in germination; when associated with S-98.
Defective in germination; when associated with V-367 and R-383.
Does not abrogate sporulation. 5-log decrease in sporulation efficiency; no ATPase activity, but no change in ATP-binding ability; unable to polymerize and fails to promote coat assembly; when associated with A-70.
Mislocalized to the mother cell cytosol. Misassembles the spore coat. Mislocalizes CotE but not SpoVM. Does not multimerize. Almost complete block in spore formation.
Spores form largely normal coats and possess at least some cortex, but cannot remain dehydrated after mother cell lysis. Abnormal synthesis and an insufficient level of spore peptidoglycan, which contains amidated diaminopimelic acid, which is normally found only in vegetative cell peptidoglycan. Defective in germination; when associated with P-256.
Suppressor mutation that reverses the mislocalization and sporulation defect of the A-6 mutation in SpoVM. Causes little impairment of sporulation.
Walker A motif; involved in ATP-binding and hydrolysis
SPbeta prophage-derived putative antirepressor protein YoqD
Uncharacterized N-acetyltransferase YobR
Uncharacterized HTH-type transcriptional regulator YezC
Abolishes induction of sigma-W-controlled genes.
No longer requires PrsW for degradation.
Partially inhibits SigW, confers low diamide induction. A swap mutant with RsrA of S.coelicolor.
Retains anti-sigma-W activity, not degraded by PrsW.
Wild-type; retains anti-sigma-W activity, degraded by PrsW.
Uncharacterized HTH-type transcriptional regulator YusO
Uncharacterized ABC transporter ATP-binding protein YybJ
SPbeta prophage-derived uncharacterized protein YorG
Uncharacterized HTH-type transcriptional regulator YwrC
Probable ribosomal RNA small subunit methyltransferase B
Putative ABC transporter ATP-binding protein AlbC
No phosphorylation by HPrK/P; abolishes the catabolite repressive effects of glucose on gluconate kinase, glucitol dehydrogenase, and the mannitol-specific catabolic enzymes.
Pros-phosphohistidine intermediate; alternate
Probable ABC transporter permease protein YqgH
Cadmium, cobalt and zinc/H(+)-K(+) antiporter
No longer inhibits growth when overexpressed in relA mutants, probably does not synthesisize (p)ppGpp. 70S ribosomes no longer dimerize to form 100S ribosomes.
No longer inhibits growth when overexpressed in relA mutants.
DNA-binding transcriptional repressor ScoC
Uncharacterized isochorismatase family protein YddQ
Gamma-D-glutamyl-L-lysine dipeptidyl-peptidase
Uncharacterized HTH-type transcriptional regulator YvdE
Uncharacterized MFS-type transporter YqjV
Probable molybdopterin-guanine dinucleotide biosynthesis adapter protein
Uncharacterized HTH-type transcriptional regulator YuxN
Does not significantly affect alpha-galacturonidase activity.
Response regulator aspartate phosphatase B
50% decrease in CSF production. 97% reduction in cleavage of pro-CSF by subtilisin and 42% reduction in cleavage by Vpr.
65% decrease in CSF production. 47% reduction in cleavage of pro-CSF by subtilisin and 79% reduction in cleavage by Vpr.
95% decrease in CSF production. 98% reduction in cleavage of pro-CSF by subtilisin and 90% reduction in cleavage by Vpr.
Competence and sporulation stimulating factor
Decreases transcription of the ComA-controlled gene rapA.
Does not stimulate expression of the srfA operon. Loss of inhibitory activity. Strong decrease in sporulation stimulation.
Does not stimulate expression of the srfA operon. No change in inhibitory activity and in sporulation stimulation.
Does not stimulate expression of the srfA operon. No change in inhibitory activity. Strong decrease in sporulation stimulation. Loss of activity toward RapB. Does not acquire any ability to inhibit RapA.
Does not stimulate expression of the srfA operon. Reduced inhibitory activity. Strong decrease in sporulation stimulation.
Increases activity toward RapB. Does not acquire any ability to inhibit RapA.
Loss of activity toward RapB. Does not acquire any ability to inhibit RapA.
Slightly increases transcription of the ComA-controlled gene rapA. No change in CSF levels.
Putative metabolite transport protein YfiG
Uncharacterized RNA pseudouridine synthase YjbO
Iron(3+)-hydroxamate import system permease protein FhuB
Metalloregulation DNA-binding stress protein
Putative carbonic anhydrase-like protein YbcF
Polyketide biosynthesis acyl-carrier-protein AcpK
Peptidoglycan-N-acetylmuramic acid deacetylase PdaA
PTS system fructose-specific EIIB component
Probable N-acetyl-LL-diaminopimelate aminotransferase
Guanosine ABC transporter permease protein NupQ
Uncharacterized HTH-type transcriptional regulator YczG
Uncharacterized HTH-type transcriptional regulator YbfP
Sodium, potassium, lithium and rubidium/H(+) antiporter
Deoxyuridine 5'-triphosphate nucleotidohydrolase YncF
Uncharacterized HTH-type transcriptional regulator YdeE
Probable guanidinium efflux system subunit GdnC
L-cystine transport system permease protein TcyB
N-acetyl-gamma-glutamyl-phosphate reductase
Carbamoyl-phosphate synthase arginine-specific large chain
Uncharacterized HTH-type transcriptional regulator YybE
SPbeta prophage-derived uncharacterized protein YorB
Helical; Signal-anchor for type II membrane protein
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagT
Loss of kinase activity towards RsbS. Loss of the ability to activate sigma-B in response to salt and ethanol stress while retaining the ability to respond to energy stress.
Bifunctional cytochrome P450/NADPH--P450 reductase 1
Exhibits modified substrate specificity. Shows approximately 6- to 9-fold increased activity with SDS, lauric acid and 1,4-naphthoquinone, and enhanced activity for other substrates such as ethacrynic acid and epsilon-amino-n-caproic acid.
Uncharacterized HTH-type transcriptional regulator YraN
Uncharacterized HTH-type transcriptional regulator YdgG
Putative membrane-bound acyltransferase YfiQ
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
ABC-type transporter ATP-binding protein EcsA
In ecsA-26; exoamylase and other exoenzymes decrease, competence- and sporulation-deficient phenotype.
Spore coat polysaccharide biosynthesis protein SpsD
SPbeta prophage-derived uncharacterized protein YoqR
Bifunctional cytochrome P450/NADPH--P450 reductase 2
Hydroxylates shorter substrates with higher conversion rates than wild-type, and in contrast to wild-type, is also able to convert medium-chain alkanes and aromatic compounds; when associated with Q-190.
Hydroxylates shorter substrates with higher conversion rates than wild-type, and in contrast to wild-type, is also able to convert medium-chain alkanes and aromatic compounds; when associated with V-89.
Protease synthase and sporulation protein PAI 2
Putative tRNA (cytidine(34)-2'-O)-methyltransferase
PTS system mannitol-specific EIICB component
Probable sodium/glutamine symporter GlnT
Abolishes negative regulation of DNA binding, without significantly interfering with the Cu(1+) affinity or coordination geometry.
Copper-sensing transcriptional repressor CsoR
Uncharacterized HTH-type transcriptional regulator YdzF
Probable amino-acid-binding protein YxeM
Uncharacterized MFS-type transporter YfiU
3-oxoacyl-[acyl-carrier-protein] synthase 2
In strain: GS77; resistant to cerulenin.
Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving system
O-acetylserine dependent cystathionine beta-synthase
Uncharacterized transmembrane protein YshB
Uncharacterized zinc-type alcohol dehydrogenase-like protein AdhB
Uncharacterized glycosyltransferase YkoT
Uncharacterized N-acetyltransferase YjcF
Uncharacterized glycosyltransferase YwdF
Probable transcriptional regulatory protein YeeI
SPbeta prophage-derived uncharacterized protein YomY
SPbeta prophage-derived uncharacterized protein YoqG
In ts-A 73; temperature-sensitive lethal mutation.
Ribonucleoside-diphosphate reductase subunit alpha
Uncharacterized MscS family protein YkuT
Putative pyruvate, phosphate dikinase regulatory protein
SPbeta prophage-derived uncharacterized protein YosD
Probable membrane transporter protein YdhB
Uncharacterized HTH-type transcriptional regulator YdfD
Decreased affinity for protoporphyrinogen-IX.
Reduces protoporphyrinogen oxidation by 90%.
Reduces protoporphyrinogen oxidation by 99%.
Oligopeptide transport system permease protein OppC
Uncharacterized HTH-type transcriptional regulator YtcD
Molybdopterin synthase sulfur carrier subunit
Slight induction of WalK-dependent genes.
Two-component system WalR/WalK regulatory protein YycH
Probable glycine dehydrogenase (decarboxylating) subunit 1
Unsaturated rhamnogalacturonyl hydrolase YesR
SPbeta prophage-derived uncharacterized protein YomO
ATP phosphoribosyltransferase regulatory subunit
Petrobactin import system permease protein YclN
D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS
Pyruvate dehydrogenase E1 component subunit beta
Putative phosphinothricin acetyltransferase YwnH
Accumulation of S-bacillithiolated form due to lack of this resolving cysteine residue. Debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) without the prior NaBH(4) reduction of this protein in vitro. Regenerates active OhrR with DNA-binding activity, but unable to activate the S-cysteinyl cysteine modified form of OhrR (OhrR-SSCys) in vitro. Debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB).
Loss of debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) by the NaBH(4)-reduced form of this protein in vitro. Loss of regeneration of active OhrR with DNA-binding activity in vitro. Loss of debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB).
SPbeta prophage-derived uncharacterized protein YopW
Schiff-base intermediate with acetaldehyde
Arabinooligosaccharides transport system permease protein AraP
Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.
Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
SPbeta prophage-derived uncharacterized protein YorT
Choline transport system permease protein OpuBB
Uncharacterized membrane protein YdzM/YouB
Inositol 2-dehydrogenase/D-chiro-inositol 3-dehydrogenase
Aminodeoxychorismate synthase component 1
Complete loss of aminodeoxychorismate synthase activity.
Eliminates Spx binding. Does not enhance Spx proteolysis.
Sporulation initiation inhibitor protein Soj
Abolishes lipid modification of the protein.
Decrease in PPIase activity. No effect on secretion of proteins and on growth.
Decreased secretion of proteins; no effect on growth.
Loss of PPIase function. No effect on secretion of proteins and on growth.
No effect on secretion of proteins and on growth.
Response regulator aspartate phosphatase E
SPbeta prophage-derived UPF0714 protein YoqZ
L-Lysine--8-amino-7-oxononanoate transaminase
Can bind ComA but is insensitive to PhrF inhibition.
Can bind ComA. Interacts with RapF, and can also interact with PhrC.
Can bind ComA. Interacts with RapF, but not with PhrC.
Completely suppresses expression from srfA promoter and is insensitive to PhrF inhibition.
Significant decrease in ComA binding activity.
Polyketide biosynthesis 3-hydroxy-3-methylglutaryl-ACP synthase PksG
Probable 4-aminobutyrate aminotransferase
2000-fold reduction in catalytic efficiency.
6300-fold reduction in catalytic efficiency.
About 2-fold decrease in substrate affinity and 30-fold reduction in catalytic activity.
About 2-fold decrease in substrate affinity and 70-fold reduction in catalytic activity.
Putative S-adenosyl-L-methionine-dependent methyltransferase YktD
Sulfite reductase [NADPH] flavoprotein alpha-component
SPbeta prophage-derived uncharacterized protein YopM
N-acetyl-S-(2-succino)cysteine monooxygenase
FMN-dependent NADH:quinone oxidoreductase 1
Extracellular matrix regulatory protein B
Fe-S protein maturation auxiliary factor YitW
Ribosomal RNA small subunit methyltransferase E
SPbeta prophage-derived uncharacterized protein YorJ
Putative acetyl-CoA C-acetyltransferase YhfS
SPbeta prophage-derived uncharacterized protein YomF
Protein-arginine kinase activator protein
2-fold decrease in affinity and very low decrease in catalytic efficiency.
Low decrease in catalytic efficiency and in affinity.
SPbeta prophage-derived uncharacterized protein YopI
Charge relay system; for urate oxidase activity
Proton donor; for OHCU decarboxylase activity
Uric acid degradation bifunctional protein PucL
SPbeta prophage-derived uncharacterized protein YoqO
3-oxoacyl-[acyl-carrier-protein] reductase FabG
Uncharacterized HTH-type transcriptional regulator YetL
Putative aliphatic sulfonates transport permease protein SsuC
2-(S-cysteinyl)pyruvic acid O-phosphothioketal
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
No longer confers resistance to virginiamycin M, still confers resistance to lincomycin and tiamycin.
No longer confers resistance to virginiamycin M.
Probably has no ATPase activity, does not confer lincomycin resistance, cells enter stationary phase at lower cell density, almost all protein is bound to ribosomes; when associated with Q-129 (called EQ2). Used for electron microscopy.
Probably has no ATPase activity, does not confer lincomycin resistance, cells enter stationary phase at lower cell density, almost all protein is bound to ribosomes; when associated with Q-432 (called EQ2). Used for electron microscopy.
Still confers resistance to virginiamycin M, lincomycin and tiamycin.
Linearmycin resistance permease protein LnrN
Uncharacterized amino acid permease YtnA
Spore coat polysaccharide biosynthesis protein SpsG
Uncharacterized ATP-dependent helicase YwqA
High-affinity zinc uptake system membrane protein ZnuB
Uncharacterized MFS-type transporter YfnC
Uncharacterized RNA pseudouridine synthase YtzG
tRNA threonylcarbamoyladenosine dehydratase
No effect on glycosylation; when associated with A-42.
No effect on glycosylation; when associated with Q-40.
SPbeta prophage-derived bacteriocin sublancin-168
Putative sporulation-specific glycosylase YdhD
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-80 and Q-83.
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-80 and Q-86.
Disrupts interaction with Bacillus phage SP01 Gp46; when associated with Q-83 and Q-86.
Weakens the interaction with Bacillus phage SP01 Gp46.
Cadmium, zinc and cobalt-transporting ATPase
Putative metabolite transport protein YncC
SPbeta prophage-derived uncharacterized protein YonV
Uncharacterized HTH-type transcriptional regulator YurK
Putative ADP-ribose pyrophosphatase YjhB
UTP--glucose-1-phosphate uridylyltransferase YngB
Probable siderophore transport system permease protein YfiZ
No activity; no imine formation with DXP.
Oligopeptide transport ATP-binding protein AppD
Iron(3+)-hydroxamate import system permease protein FhuG
Proton acceptor; specific for (R)-substrate epimerization
Proton acceptor; specific for (S)-substrate epimerization
Spore germination protein-like protein YdzR
Uncharacterized HTH-type transcriptional regulator YceK
Uncharacterized ABC transporter ATP-binding protein YfmM
Uncharacterized HTH-type transcriptional regulator YtdP
Uncharacterized MFS-type transporter YubD
PTS system oligo-beta-mannoside-specific EIIB component
Uncharacterized N-acetyltransferase YesJ
SPbeta prophage-derived uncharacterized protein YonI
6-phospho-5-dehydro-2-deoxy-D-gluconate aldolase
Uncharacterized MFS-type transporter YwoD
Energy-coupling factor transporter ATP-binding protein EcfA2
Sporulation integral membrane protein YlbJ
Putative sensory transducer protein YvaQ
SPbeta prophage-derived uncharacterized protein YomV
Loss of both kinase and phosphorylase activities.
Nearly no effect on both kinase and phosphorylase activities.
No effect on ATP-dependent kinase activity, but loss of both PPi-kinase and phosphorylase activities.
No effect on both kinase and phosphorylase activities.
Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity
Reduced kinase activity and loss of phosphorylase activity.
Reduced kinase activity and loss of phosphorylase activity. No effect on FBP binding.
Slight reduction in both kinase and phosphorylase activities.
SPbeta prophage-derived uncharacterized protein YopZ
Uncharacterized HTH-type transcriptional regulator YobV
Uncharacterized FAD-linked oxidoreductase YvdP
Small, acid-soluble spore protein gamma-type
Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
PTS system mannose-specific EIIBCA component
Constitutively active; causes constitutive activation of SigW.
SPbeta prophage-derived uncharacterized transglycosylase YomI
Probable glutamine ABC transporter permease protein GlnM
Uncharacterized HTH-type transcriptional regulator YcgK
Uncharacterized MFS-type transporter YttB
KinB-signaling pathway activation protein
SPbeta prophage-derived aminoglycoside N(3')-acetyltransferase-like protein YokD
Loss of catalytic activity. Inability to form the acyl-enzyme intermediate.
Loss of copper binding; when associated with S-13.
Loss of copper binding; when associated with S-16.
Spore coat polysaccharide biosynthesis protein SpsE
SPbeta prophage-derived probable integrase/recombinase YopP
Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha
Resistance to polyketide antibiotic thailandamide.
Resistant to the pyrrolidine dione antibiotic CPD2.
Probable amino-acid permease protein YxeN
SPbeta prophage-derived uncharacterized protein YorM
SPbeta prophage-derived glycosyltransferase SunS
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
SPbeta prophage-derived uncharacterized protein YopF
Probable 3-hydroxybutyryl-CoA dehydrogenase
Menaquinol-cytochrome c reductase cytochrome b subunit
Strongly reduces catalytic efficiency and enzyme activity. Slightly increases substrate affinity.
Strongly reduces catalytic efficiency while increasing substrate affinity.
Impaired enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
Loss of enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
Very low enzymatic activity. Great decrease in biofilm formation. Deformed cell morphology.
SPbeta prophage-derived uncharacterized protein YorC
Redox-sensing transcriptional repressor Rex
Uncharacterized N-acetyltransferase YvbK
Putative teichuronic acid biosynthesis glycosyltransferase TuaH
Uncharacterized MFS-type transporter YvkA
Changes the metal specificity of the enzyme. Can insert Co(2+) into protoporphyrin IX at a higher rate than the wild-type enzyme, but loses the ability to insert Cu(2+) and Zn(2+).
Uncharacterized transcriptional regulatory protein YvcP
Destabilization of structural stability and loss of functionality.
Uncharacterized MFS-type transporter YybF
Uncharacterized RNA pseudouridine synthase YhcT
SPbeta prophage-derived uncharacterized N-acetyltransferase YokL
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Uncharacterized MFS-type transporter YdgK
SPbeta prophage-derived uncharacterized lipoprotein YonS
Leads to copper binding and enzyme activity; when associated with H-104.
Leads to copper binding and enzyme activity; when associated with H-88.
N-(5'-phosphoribosyl)anthranilate isomerase
Uncharacterized ABC transporter ATP-binding protein YtlC
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-2 or A,Q-4. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-2 and A,Q-4.
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-2 or A,Q-5. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-2 and A,Q-5.
Cross-linking not affected. Cross-linking not affected; when associated with A,Q-4 or A,Q-5. Cross-linking eliminated, but still localizes CwlJ properly; when associated with A,Q-4 and A,Q-5.
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-?)
Glyceraldehyde-3-phosphate dehydrogenase 2
Uncharacterized HTH-type transcriptional regulator YxjO
SPbeta prophage-derived uncharacterized protein YoqS
Uncharacterized UDP-glucose epimerase YtcB
Methyl-accepting chemotaxis protein McpA
No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-593.
No CheD modification at Q-593 and Q-594 but modification possible at the unidentified second site; when associated with A-594.
Loss of protease activity, but mutant is still able to swarm.
10-fold reduction in glutaminase activity.
3-fold reduction in glutaminase activity.
Almost no effect on glutaminase activity.
No activity, disturbing interaction with PdxS.
Pyridoxal 5'-phosphate synthase subunit PdxT
Uncharacterized N-acetyltransferase YbbJ
Type II restriction enzyme BsuMI component YdiR
10% of wild-type activity. 17-fold decrease in the affinity for the pyrimidine substrate, but no effect on that for 1-deoxy-L-glycero-tetrulose 4-phosphate.
Does not complement the tagTUV deletion mutant.
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagU
SPbeta prophage-derived uncharacterized protein YotN
18-fold reduction in p-Arg phosphatase activity and 11-fold increase in p-Tyr phosphatase activity.
18-fold reduction in p-Arg phosphatase activity and 22-fold increase in p-Tyr phosphatase activity.
60-fold reduction in p-Arg phosphatase activity and 4-fold reduction in p-Tyr phosphatase activity.
Completely loss of phosphatase activity.
Phosphate import ATP-binding protein PstB 1
SPbeta prophage-derived uncharacterized protein YonF
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Peptide of residues 247-265 no longer binds FliW.
Heptaprenyl diphosphate synthase component 1
Putative cytochrome bd menaquinol oxidase subunit I
Uncharacterized MFS-type transporter YwoG
SPbeta prophage-derived uncharacterized protein YoqH
Uncharacterized HTH-type transcriptional regulator YezE
ATP-dependent helicase/deoxyribonuclease subunit B
Loss of iron-sulfur group binding, loss of DNA-binding.
Some loss of nuclease activity, helicase and DNA-binding are unaltered; when associated with A-1172 in AddA nearly complete loss of nuclease activity.
DNA-directed RNA polymerase subunit alpha
Methyl-accepting chemotaxis protein TlpA
SPbeta prophage-derived uncharacterized lipoprotein YokB
Bifunctional riboflavin kinase/FMN adenylyltransferase
Cryptic catabolic NAD-specific glutamate dehydrogenase GudB
In gudB1; gains glutamate dehydrogenase activity, restores growth on proline, arginine, ornithine.
D-alanine--D-alanyl carrier protein ligase
Uncharacterized N-acetyltransferase YlbP
Uncharacterized MFS-type transporter YddS
SPbeta prophage-derived uncharacterized protein YonB
Methionine import ATP-binding protein MetN
Teichoic acid glycerol-phosphate primase
Fe(3+)-citrate import system permease protein YfmE
Sporulation initiation phosphotransferase B
Spore morphogenesis and germination protein YwcE
Probable ABC transporter permease protein YesQ
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
SPbeta prophage-derived DNA-binding protein HU 2
Cysteine sulfenic acid (-SOH); alternate
Full repressor activity, but no modulation by peroxide.
N,N-(cysteine-1,S-diyl)phenylalanine (Cys-Phe); alternate
Organic hydroperoxide resistance transcriptional regulator
S-bacillithiol cysteine disulfide; alternate
SPbeta prophage-derived uncharacterized protein YopX
Methylated-DNA--protein-cysteine methyltransferase, inducible
Germination-specific N-acetylmuramoyl-L-alanine amidase
Uncharacterized MFS-type transporter YuxJ
Sec-independent protein translocase protein TatCy
Sporulation initiation phosphotransferase F
4-hydroxy-tetrahydrodipicolinate synthase
Teichuronic acid biosynthesis protein TuaF
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Sporulation inhibitor of replication protein SirA
Partially restores cytochrome c synthesis in a CcdA-deficient mutant, possibly because the bacteria can no longer oxidize the 2 heme-binding thiol groups in apocytochrome c.
Uncharacterized MscS family protein YfkC
Loss of phosphorylation. Interacts strongly with RsbW.
Mg(2+)/citrate complex secondary transporter
Probable xanthine dehydrogenase subunit C
Methylated-DNA--protein-cysteine methyltransferase, constitutive
Uncharacterized HTH-type transcriptional regulator YmfC
Causes a severe defect in cell morphology and in chromosome segregation. Can still form filamentous structures, but these are rigid rather than dynamic.
Uncharacterized HTH-type transcriptional regulator YvaF
Uncharacterized transcriptional regulatory protein YkoG
SkfA peptide export ATP-binding protein SkfE
ABC transporter ATP-binding protein YtrE
Type II restriction enzyme BsuMI component YdjA
Probable membrane transporter protein YrkJ
Uncharacterized transmembrane protein YxlG
D-alanyl-D-alanine carboxypeptidase DacC
Loss of aconitase activity. It is glutamate auxotroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of inactive aconitase.
Same aconitase activity compared to the wild-type. It is glutamate prototroph and accumulates citrate. Exhibits overexpression of the citB promoter and accumulates high levels of active aconitase.
Putative UDP-glucose 6-dehydrogenase YtcA
Uncharacterized ABC transporter extracellular-binding protein YurO
Impairs sporulation inhibition activity.
In spo0E11; a hyperactive phosphatase, it is no longer a substrate for FtsH.
Putative penicillin-binding protein PbpX
SPbeta prophage-derived uncharacterized protein YolC
Uncharacterized HTH-type transcriptional regulator YvaP
Probable threonine/serine transporter YbxG
2-oxoglutarate dehydrogenase E1 component
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase
Spermine/spermidine N(1)-acetyltransferase
Cytochrome bd ubiquinol oxidase subunit 2
High-affinity zinc uptake system ATP-binding protein ZnuC
SPbeta prophage-derived uncharacterized protein YokE
Probable ABC transporter permease protein YqgI
Uncharacterized HTH-type transcriptional regulator YhbI
Uncharacterized glycosyltransferase YkoN
Methyl-accepting chemotaxis protein TlpC
Retains the ability to degrade haem, albeit at a reduced rate relative to the wild-type.
Probable inorganic carbon transporter subunit DabA
Uncharacterized HTH-type transcriptional regulator YuzN
Thiazolidine linkage to a ring-opened DNA abasic site
Probable glucitol transport protein GutA
Uncharacterized transcriptional regulatory protein YvfU
Uncharacterized MFS-type transporter YceJ
Antilisterial bacteriocin subtilosin biosynthesis protein AlbB
Poly(glycerol-phosphate) alpha-glucosyltransferase
Sigma factor SigB regulation protein RsbQ
Ability to bypass the requirement of SdpC binding for expression of the sdpRI genes.
Loss of ability to promote transcription of the sdpRI genes in response to SdpC binding. No effect in ability to confer immunity to SdpC.
Putative HTH-type DNA-binding domain-containing acetyltransferase YbfA
Uncharacterized HTH-type transcriptional regulator YfiF
At least 50-fold decrease of the second-order rate constant for the acylation step.
No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
Extracellular matrix regulatory protein A
Probable spore germination protein GerPD
N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase
Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]
Negative regulator of flagellin synthesis
scyllo-inositol 2-dehydrogenase (NADP(+)) IolU
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
TrbL/VirB6 plasmid conjugal transfer protein
Uncharacterized HTH-type transcriptional regulator YoaU
Putative polyketide biosynthesis enoyl-CoA isomerase PksI
Loss of phosphorylation and loss of the ability to respond to salt stress. Interacts strongly with RsbT.
Putative metabolite transport protein YwtG
Probable peptide export permease protein YydJ
Uncharacterized SPbeta prophage-derived protein YotG
Putative sensory transducer protein YfmS
Lichenan-specific phosphotransferase enzyme IIA component
Tele-phosphohistidine intermediate; by HPr
Guanosine ABC transporter permease protein NupP
Enzyme activity changes to monoacylglycerol hydrolase. Marked decrease in temperature stability, decreased stability at pH 11 but increased stability at pH 5.
Putative membrane protein insertion efficiency factor
Uncharacterized HTH-type transcriptional regulator FruR
Uncharacterized HTH-type transcriptional regulator YhdI
Uncharacterized HTH-type transcriptional regulator YgzD
Uncharacterized MFS-type transporter YfkL
In spo-1; no spores develop, no induction of sporulation-associated enzymes.
In spo-560; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-561; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-563; 10(6)-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-564; 1000-fold decrease in spore production, 50% induction of sporulation-associated enzymes.
In spo-578; no spores develop, no induction of sporulation-associated enzymes.
Chromosomal replication initiator protein DnaA
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
PTS system glucose-specific EIICBA component
Cyclic pyranopterin monophosphate synthase
Probable multidrug resistance protein YoeA
Probable undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase
SPbeta prophage-derived putative HNH homing endonuclease YosQ
Probable NADH-dependent butanol dehydrogenase 1
Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit beta
Putative HTH-type transcriptional regulator YwgB
Asparagine synthetase [glutamine-hydrolyzing] 3
Response regulator aspartate phosphatase A
Retains phosphatase activity, but is insensitive to PhrA inhibition.
Sporulation-defective phenotype. Retains phosphatase activity, but is insensitive to PhrA inhibition.
Uncharacterized MFS-type transporter YfmI
Release factor glutamine methyltransferase
Uncharacterized metal-dependent hydrolase YabD
Complete loss of ATPase activity; mutant proteins trap ATP in a vanadate-independent manner whereas the wild-type protein traps ADP.
Multidrug resistance ABC transporter ATP-binding/permease protein BmrA
Retains 2% ATPase activity; unable to transport Hoechst 33342. Traps ADP in a beryllium fluoride-dependent manner, confirming ATPase activity. Probably unable to undergo NBD dimerization.
Probable serine/threonine-protein kinase YbdM
Uncharacterized ATP-dependent helicase YprA
No activity, but can, as the wild-type, form covalent adduct with ribose-5-phosphate.
No activity, does not form covalent adduct with ribose-5-phosphate or ribulose 5-phosphate.
No activity, does not form covalent adduct with ribose-5-phosphate.
Pyridoxal 5'-phosphate synthase subunit PdxS
Results in 20-fold reduction of activity.
Schiff-base intermediate with D-ribose 5-phosphate
SPbeta prophage-derived uncharacterized protein YosI
Two-component system WalR/WalK regulatory protein YycI
SPbeta prophage-derived uncharacterized membrane protein YosE
Uncharacterized HTH-type transcriptional regulator YqaF
Pyruvate dehydrogenase E1 component subunit alpha
Uncharacterized N-acetyltransferase YtmI
Carbamoyl-phosphate synthase arginine-specific small chain
Assimilatory nitrate reductase catalytic subunit
N-acetylmuramic acid 6-phosphate etherase
Aerobic glycerol-3-phosphate dehydrogenase
Probable 2-phosphosulfolactate phosphatase
Methylthioribulose-1-phosphate dehydratase
Impaired chemotaxis. No binding to CheD. Almost no enhancement of CheC activity by CheD.
Phosphoribosylformylglycinamidine synthase subunit PurS
Putative arsenical pump membrane protein
1-pyrroline-5-carboxylate dehydrogenase 2
N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase
Putative type II methyltransferase M.BsuMIIP
Transcription termination/antitermination protein NusG
Arginine-binding extracellular protein ArtP
Beta-ketoacyl-[acyl-carrier-protein] synthase III 1
Bifunctional transcriptional activator/DNA repair enzyme AdaA
Nucleophile; methyl group acceptor from methylphosphotriester
Abolishes 3'-5' exonuclease activity. Still possesses DNA polymerase activity.
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YwtE
Loss of ability to regulate the pyr operon and to bind to RNA; no effect on UPRTase activity.
Loss of ability to regulate the pyr operon; highly reduced affinity for RNA; no effect on UPRTase activity.
Loss of ability to regulate the pyr operon; no effect on UPRTase activity.
No effect on ability to regulate the pyr operon only in presence of excess pyrimidines; reduced affinity for RNA; no effect on UPRTase activity.
No effect on ability to regulate the pyr operon; no effect on uprtase activity.
Reduced ability to regulate the pyr operon, and loss of ability to bind to RNA; no effect on UPRTase activity.
Reduced ability to regulate the pyr operon; decreased UPRTase activity.
Reduced ability to regulate the pyr operon; reduced affinity for RNA; loss of UPRTase activity.
Uncharacterized HTH-type transcriptional regulator YhcF
SPbeta prophage-derived uncharacterized protein YotJ
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Uncharacterized ABC transporter ATP-binding protein YfiC
Significant decrease of isomerase activity.
ATP-dependent Clp protease proteolytic subunit
Has peptidase but not protease activity, activated by ADEP antibiotics.
Has peptidase but not protease activity, partially activated by ADEP antibiotics.
Has peptidase but not protease activity.
Protein no longer oligomerizes, loss of protease activity, activated by ADEP antibiotics.
Protein no longer oligomerizes, loss of protease activity.
Probable queuosine precursor transporter
Polyketide biosynthesis malonyl-ACP decarboxylase PksF
Putative ABC transporter ATP-binding protein YjkB
Able to complement for the deletion of this protein.
Loss of ATPase activity. Loss of ability to complement for the deletion of this protein.
Loss of ability to complement for the deletion of this protein.
Loss of ability to complement for the deletion of this protein. Loss of native structure.
No effect on susceptibility to DNA damage.
Uncharacterized HTH-type transcriptional regulator YpoP
Linearmycin resistance permease protein LnrM
Oligopeptide transport system permease protein OppB
Glucose-1-phosphate thymidylyltransferase
Cleaves SAM, but does not form thioether bond, contains 52% iron of wild-type.
No longer cleaves SAM, contains 55% iron of wild-type.
Sporulation killing factor maturation protein SkfB
Uncharacterized HTH-type transcriptional regulator YerO
Uncharacterized N-acetyltransferase YnaD
Molybdopterin synthase catalytic subunit
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB
Putative SPbeta prophage-derived single-strand DNA-specific exonuclease YorK
Uncharacterized beta-barrel protein YwiB
DEAD-box ATP-dependent RNA helicase CshB
Uncharacterized HTH-type transcriptional regulator YtzE
Peptidase_MA_2 domain-containing protein
Uncharacterized MFS-type transporter YxlH
In mut9; sporulation efficiency decreases by about 4 orders of magnitude.
Carbamoyl-phosphate synthase pyrimidine-specific large chain
SPbeta prophage-derived uncharacterized protein YosX
SPbeta prophage-derived uncharacterized protein YouA
Probable siderophore-binding lipoprotein YfiY
Prepilin leader peptidase/N-methyltransferase
SPbeta prophage-derived uncharacterized protein YorA
Putative pyridoxal phosphate-dependent aminotransferase EpsN
Purine nucleoside transport protein NupG
Polygalacturonan/rhamnogalacturonan transport system permease protein YteP
Glycerol uptake operon antiterminator regulatory protein
Increases accumulation of full-length MifM.
Increases accumulation of full-length MifM. Reduces misCB/yqjG induction in misCA/spoIIIJ mutant.
Membrane protein insertion and folding monitor
Reduces misCB/yqjG induction in misCA/spoIIIJ mutant.
Glutathione/L-cysteine transport system ATP-binding/permease protein CydC
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YcsE
Does not affect septal localization and growth.
Does not affect septal localization; when associated with R-37 and R-41.
Does not affect septal localization; when associated with R-37 and R-44.
Does not affect septal localization; when associated with R-41 and R-44.
Ribosomal RNA small subunit methyltransferase I
Uncharacterized ABC transporter permease YvcS
N5-carboxyaminoimidazole ribonucleotide synthase
Probable polysaccharide biosynthesis protein EpsC
Proline-responsive transcriptional activator PutR
SPbeta prophage-derived uncharacterized protein YosL
Prevents inactivation of CtsR at high temperature.
Uncharacterized MFS-type transporter YfhI
PTS system sucrose-specific EIIBC component
Putative polysaccharide deacetylase YheN
Probable murein peptide carboxypeptidase
Uncharacterized HTH-type transcriptional regulator YwhA
In JH17041; thermosensitive; decrease in dimerization and DNA-binding.
Probable 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
Succinate dehydrogenase cytochrome b558 subunit
Uncharacterized ABC transporter ATP-binding protein YlmA
Degradation enzyme regulation protein DegQ
SPbeta prophage-derived uncharacterized protein YomW
Uncharacterized MFS-type transporter YxaM
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Uncharacterized HTH-type transcriptional regulator YobQ
ABC transporter glutamine-binding protein GlnH
Uncharacterized MFS-type transporter YwfA
Glutathione hydrolase-like YwrD large chain
Glutathione hydrolase-like YwrD small chain
SPbeta prophage-derived uncharacterized protein YosB
Loss of activity. Reduces activity 500-fold, without effect on zinc binding; when associated with Q-56.
Loss of activity. Reduces zinc binding by 80%.
No effect on zinc binding. Strongly reduces Vmax.
No effect on zinc binding. Strongly reduces Vmax. Reduces activity 500-fold; when associated with H-53.
Teichuronic acid biosynthesis protein TuaB
L-cystine transport system permease protein TcyL
2-oxoisovalerate dehydrogenase subunit alpha
SPbeta prophage-derived uncharacterized protein YorN
Uncharacterized N-acetyltransferase YhfO
Loss of enzymatic activity. Fails to form the normal DisA focus. Cannot trigger the DNA damage response. Increased sensitivity to methyl methanesulfonate.
Reduction in the capability of the four-way junction DNA to inhibit diadenylate cyclase. Reduction in DNA-binding.
Binds DNA, but loss of arsenite-mediated regulation; when associated with S-33.
Binds DNA, but loss of arsenite-mediated regulation; when associated with S-35.
Imidazole glycerol phosphate synthase subunit HisH
SPbeta prophage-derived uncharacterized protein YoqK
Loss of phosphodiesterase activity, does not confer antibiotic sensitivity when overexpressed.
Uncharacterized sodium-dependent transporter YhdH
SPbeta prophage-derived uncharacterized protein YorR
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Manganese-dependent inorganic pyrophosphatase
3-fold reduction in catalytic efficiency.
4-fold reduction in catalytic efficiency.
Did not get expressed in heterologous host.
Teichoic acid poly(glycerol phosphate) polymerase
Loss of ability to recognize wild-type spoVG promoter. Suppressor of the spoVG249 mutation in the -10 region of the spoVG promoter.
Suppressor of mutations in the -12 region of the spoVG promoter.
Suppressor of mutations in the -13 region of the spoVG promoter.
Probable serine/threonine-protein kinase YabT
Uncharacterized MFS-type transporter YvmA
Molybdenum cofactor biosynthesis protein B
Menaquinol-cytochrome c reductase cytochrome b/c subunit
SPbeta prophage-derived uncharacterized protein YoqA
Probable ABC transporter extracellular-binding protein YckB
Probable biofilm-surface layer protein B
Molybdopterin-synthase adenylyltransferase
Short-chain dehydrogenase/reductase homolog YusR
SPbeta prophage-derived stress response protein SCP1
Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase
Uncharacterized metallophosphoesterase YhaO
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating
Putative peptidoglycan O-acetyltransferase YrhL
No longer binds tRNA(Ala) anticodon (UGC), decreased accumulation of a stalled reporter protein.
No longer binds tRNA(Ala) anticodon (UGC), has minor Ala tailing ability, decreased accumulation of a stalled reporter protein.
No longer binds tRNA(Ala) anticodon (UGC), loss of Ala tailing ability, decreased accumulation of a stalled reporter protein.
DNA-directed RNA polymerase subunit beta'
In streptolydigan resistant alleles stl6/stl445.
Changes the activity from exo- to endo-cleavage.
Uncharacterized HTH-type transcriptional regulator YazB
23S rRNA (uracil-C(5))-methyltransferase RlmCD
Uncharacterized HTH-type transcriptional regulator YdeC
In grl-15; loss of glucose repression, deficiency in glucose transport and absence of ptsG expression.
In grl-30; loss of glucose repression, deficiency in glucose transport and absence of ptsG expression.
SPbeta prophage-derived uncharacterized protein YopQ
Spore coat polysaccharide biosynthesis protein SpsA
Uncharacterized ABC transporter permease YknZ
Uncharacterized MFS-type transporter YbcL
SPbeta prophage-derived uncharacterized protein YoqT
Uncharacterized isochorismatase family protein YrdC
Uncharacterized HTH-type transcriptional regulator YhjH
Arginine transport system permease protein ArtQ
Oligosaccharides import ATP-binding protein MsmX
SPbeta prophage-derived uncharacterized protein YomZ
Putative oligopeptide transport ATP-binding protein YkfD
SPbeta prophage-derived uncharacterized protein YopG
L-cystine import ATP-binding protein TcyC
Uncharacterized HTH-type transcriptional regulator YwoH
Retains less than 10% of wild-type activity.
Uncharacterized MFS-type transporter YxiO
Putative DNA-3-methyladenine glycosylase YfjP
ATP-dependent dethiobiotin synthetase BioD
Probable galactarate dehydratase (L-threo-forming)
Biotin carboxyl carrier protein of acetyl-CoA carboxylase
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Uncharacterized transcriptional regulatory protein YcbL
Uncharacterized HTH-type transcriptional regulator YvzC
Probable ABC transporter permease protein YesP
Formate-dependent phosphoribosylglycinamide formyltransferase
Iron(3+)-hydroxamate-binding protein FhuD
Does not bind the ilvB and dpp promoters.
Is predominantly monomeric. Retains the capacity to bind to DNA and repress transcription in vivo, even though its activity is considerably lower than that of the wild-type strain.
Loss of ilvB repressing activity in a BCAA-containing medium.
Loss of ilvB repressing activity in a BCAA-containing medium. DNA binding is stimulated by GTP, but not by branched-chain amino acids.
Nearly complete loss of ilvB repressing activity in a BCAA-containing medium.
No change in affinity for ilvB and dpp promoters.
Retains 72% of ilvB repressing activity in a BCAA-containing medium.
Retains some ilvB repressing activity in a BCAA-containing medium.
Retains some ilvB repressing activity in a BCAA-containing medium. Retains the ability to respond to GTP, but responds poorly to branched-chain amino acids unless GTP is simultaneously present.
Slightly reduced affinity for ilvB and dpp promoters.
Strong defect in ability to interact with the ilvB and dpp promoters.
Strongly affects the ilvB repressing activity in a BCAA-containing medium. DNA binding is stimulated by GTP, but not by branched-chain amino acids.
SPbeta prophage-derived uncharacterized protein YoqI
Sporulation-specific extracellular nuclease
Prespore-specific transcriptional regulator RsfA
Uncharacterized transcriptional regulatory protein YxjL
Putative sugar dehydratase/epimerase YfnG
Putative ATP-dependent DNA helicase YjcD
SPbeta prophage-derived uncharacterized protein YomP
SPbeta prophage-derived uncharacterized protein YonT
Putative maltodextrin utilization protein YvdJ
Probable 4-hydroxyphenylacetate 3-monooxygenase
Hydrolysis of the covalent glycosyl-enzyme intermediate is slowed.
Uncharacterized HTH-type transcriptional regulator YwcC
Uncharacterized NAD(P)H oxidoreductase YdeQ
Oxygen-independent coproporphyrinogen-III oxidase-like protein HemZ
Histidine biosynthesis bifunctional protein HisIE
SPbeta prophage-derived uncharacterized protein YopY
Uncharacterized ABC transporter ATP-binding protein YfiB
Uncharacterized HTH-type transcriptional regulator YusT
Sporulation-specific N-acetylmuramoyl-L-alanine amidase
Putative 23S rRNA (guanine-N(1)-)-methyltransferase YxjB
Required to activate transcription of the adhA-yraA operon.
ABC transporter ATP-binding protein NatA
SPbeta prophage-derived uncharacterized protein YomG
Probable flagellar hook-length control protein
N-acetyl-alpha-D-glucosaminyl L-malate deacetylase 1
Probable ATP-dependent DNA helicase RecQ
Malonyl CoA-acyl carrier protein transacylase
Fe(3+)-citrate import ATP-binding protein YfmF
Putative aliphatic sulfonates-binding protein
Putative mannose-6-phosphate isomerase YvyI
Succinate dehydrogenase flavoprotein subunit
Putative HMP/thiamine-binding protein YkoF
Linearmycin resistance ATP-binding protein LnrL
Ribosomal RNA small subunit methyltransferase G
Arabinooligosaccharides transport system permease protein AraQ
Negative impact in the growth rate in the presence of arabinotriose.
High-affinity zinc uptake system binding-protein ZnuA
Holliday junction ATP-dependent DNA helicase RuvA
Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Organic hydroperoxide resistance protein OhrA
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ
SPbeta prophage-derived uncharacterized protein YopT
Probable galactarate/D-glucarate transporter GudP
Uncharacterized HTH-type transcriptional regulator YkoM
Transcription antitermination protein NusB
Uncharacterized HTH-type transcriptional regulator YfiV
S-(2-succino)cysteine N-acetyltransferase
SPbeta prophage-derived putative HNH endonuclease YoqL
Spermidine/spermine N(1)-acetyltransferase
Putative niacin/nicotinamide transporter NaiP
Uncharacterized MFS-type transporter YhjO
Uncharacterized N-acetyltransferase YdgE
tRNA (adenine(22)-N(1))-methyltransferase
Uncharacterized HTH-type transcriptional regulator YdeS
SPbeta prophage-derived uncharacterized protein YopJ
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
Oligopeptide transport system permease protein AppC
23S rRNA (guanosine(2553)-2'-O)-methyltransferase RlmP
6-carboxy-5,6,7,8-tetrahydropterin synthase
SPbeta prophage-derived uncharacterized membrane protein YotH
7-fold decrease in induction of SdpI, decreased toxicity of SDP.
Reduced amounts of pro-SdpC produced, no proSdpC is secreted, decreased amounts of SdpI expressed, decreased toxicity.
Protein-glutamate methylesterase/protein-glutamine glutaminase
Succinate--CoA ligase [ADP-forming] subunit alpha
DNA-directed RNA polymerase subunit epsilon
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
SPbeta prophage-derived uncharacterized protein YoqB
Probable spore germination protein GerPE
Melibiose/raffinose/stachyose import permease protein MelD
Uncharacterized HTH-type transcriptional regulator YdhC
Uncharacterized ABC transporter ATP-binding protein YhcH
Uncharacterized HTH-type transcriptional regulator YydK
Required for cell division in the presence of a transpeptidase-inactive copy of PBP-2B. Loss of oxacillin and cephalexin resistance.
Uncharacterized HTH-type transcriptional regulator RghRB
SPbeta prophage-derived uncharacterized protein YonX
Uncharacterized MFS-type transporter YtbD
Cannot induce expression of trxA and trxB. Does not affect stability.
Confers defects in Spx-activated transcription but not in Spx-dependent repression.
Confers defects in Spx-activated transcription but not in Spx-dependent repression. Does not affect binding to SpxH/YjbH.
Decreases activity. 50% decrease in binding to SpxH/YjbH. Enhanced proteolysis by ClpXP in the absence of SpxH/YjbH.
Decreases activity. 80% decrease in binding to SpxH/YjbH. Enhanced proteolysis by ClpXP in the absence of SpxH/YjbH.
Decreases activity. 90% decrease in binding to SpxH/YjbH.
Retains activity. Resistant to degradation by ClpXP.
Glutamine transport ATP-binding protein GlnQ
Proton donor; for beta-elimination activity
Proton donor; for delta-elimination activity
SPbeta prophage-derived uncharacterized protein YosV
Binds only one manganese ion, in a pseudo-hexacoordinate geometry.
Retains selectivity for activation by Mn(2+) and Cd(2+) over Co(2+) and Fe(2+). Can bind Mn(2+) in the C site, despite alteration to the A site, and adopt active DNA-binding conformations. Non-cognate ions fail to generate fully active complexes.
DNA-directed RNA polymerase subunit beta
Putative multidrug export ATP-binding/permease protein YgaD
Flagellar hook-basal body complex protein FliE
SPbeta prophage-derived uncharacterized protein YonJ
Galactose-1-phosphate uridylyltransferase
Strongly reduced catalytic activity and reduced affinity for substrate.
Iron(3+)-hydroxamate import ATP-binding protein FhuC
2-oxoisovalerate dehydrogenase subunit beta
SPbeta prophage-derived uncharacterized protein YopR
Bacitracin export ATP-binding protein BceA
Ribosomal RNA large subunit methyltransferase H
Pentaglycyl murein peptidoglycan amidated threonine
Sporulation-killing factor biosynthesis protein SkfC
Glycine betaine/carnitine/choline transport system permease protein OpuCB
Prevents intramolecular processing to an active enzyme.
SPbeta prophage-derived uncharacterized protein YomS
Loss of (p)ppGpp synthesis, protein still forms tetramers.
No stimulation by (p)ppGpp, protein still forms tetramers.
Putative transcriptional regulator protein YobU
Energy-coupling factor transporter ATP-binding protein EcfA1
Putative NADP-dependent oxidoreductase YfmJ
In rpsD1; suppresses S12 mutation K55D. A ram mutation.
In rpsD2; suppresses S12 mutation K55D. A ram mutation.
2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
Uncharacterized N-acetyltransferase YjdG
8000-fold reduction in catalytic activity.
Sulfur carrier protein ThiS adenylyltransferase
Carboxy-terminal processing protease CtpA
SPbeta prophage-derived uncharacterized protein YopH
(S)-ureidoglycine--glyoxylate transaminase
5-fold decrease in transamination activity.
9-fold decrease in transamination activity.
Catabolic NAD-specific glutamate dehydrogenase RocG
Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Increase of thermostability 8 degrees Celsius higher than that of the wild-type.
Reduces the affinity for glutamate and ammonium.
Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
Heat-inducible transcription repressor HrcA
Mannitol-specific phosphotransferase enzyme IIA component
No effect on DNA binding. Results in a weak constitutive ManR activity.
No reduction in DNA-binding activity can be observed in the presence of EIIBA-Man. Leads to a nearly constitutive ManR activity.
Response regulator aspartate phosphatase J
Shows wild-type phosphatase activity and sensitivity to PhrC inhibition.
Shows wild-type phosphatase activity but is insensitive to PhrC inhibition.
Probable ATP-dependent DNA helicase RecS
SPbeta prophage-derived uncharacterized membrane protein YomJ
Uncharacterized HTH-type transcriptional regulator YxbF
Sec translocon accessory complex subunit YrbF
No or little effects on GlcNAc6P binding.
Phosphoribosylaminoimidazole-succinocarboxamide synthase
SPbeta prophage-derived uncharacterized protein YolA
ATP-dependent Clp protease ATP-binding subunit ClpE
Uncharacterized aromatic compound monooxygenase YhjG
Probable glucose-1-phosphate cytidylyltransferase
95% loss of activity, normal PLP binding capacity.
More than 95% loss of activity, and half of normal PLP binding capacity.
Probable membrane transporter protein YtnM
Probable amino-acid import ATP-binding protein YxeO
Uncharacterized MFS-type transporter YfiS
Putative HMP/thiamine permease protein YkoC
Wild-type growth rate, this enzyme probably has no synthase activity but can still degrade pppGpp to ppGpp.
Spore coat polysaccharide biosynthesis protein SpsB
Uncharacterized MFS-type transporter YhcA
1,4-dihydroxy-2-naphthoate octaprenyltransferase
Uncharacterized ABC transporter ATP-binding protein YfmR
SPbeta prophage-derived disulfide bond formation protein B
Probable transcriptional regulatory protein YrbC
Probable tartrate dehydrogenase/decarboxylase
FMN-dependent NADH:quinone oxidoreductase 2
SPbeta prophage-derived uncharacterized protein YokC
Uncharacterized HTH-type transcriptional regulator YulB
Complete loss of ability to inhibit KinA autophosphorylation.
Partial loss of ability to inhibit KinA autophosphorylation.
SPbeta prophage-derived uncharacterized protein YotK
Homooligomerizes poorly, decreased number of foci.
Homooligomerizes, no change in number of foci.
No longer homooligomerizes, poor aggregation, severe decrease in the number of foci.
No longer homooligomerizes, poor aggregation, severe decrease in the number of foci. Protein is dispersed in the cell membrane.
10-fold reduction in sporulation efficiency. Sigma factor G (SigG) is not activated, signal sequence is not cleaved.
200-fold reduction in sporulation efficiency.
7-fold reduction in sporulation efficiency (first SpoIVFB transmembrane region).
No change in sporulation efficiency (SleB signal sequence).
1000-fold decrease of the affinity for NrgAB compared to the wild-type. Structure more asymmetric than that of the wild-type.
Causes constitutive expression of TnrA. Feedback-inhibited GlnA has a greatly reduced ability to block the DNA binding.
Causes constitutive expression of TnrA. Feedback-inhibited GlnA has a greatly reduced ability to block the DNA-binding.
No interaction with glutamine synthetase.
SPbeta prophage-derived uncharacterized protein YoqE
2',3'-cyclic-nucleotide 2'-phosphodiesterase
Abolishes phosphodiesterase activity. Overexpresses flagellin and forms smooth colonies. Does not affect stability of the protein.
Increases cAMP levels. Displays aberrant colony morphologies.
Arabinogalactan endo-beta-1,4-galactanase
3-oxo-glucose-6-phosphate:glutamate aminotransferase
Uncharacterized HTH-type transcriptional regulator YdgJ
Putative membrane-bound acyltransferase YkrP
Grows slowly, very reduced association with ribosomes, fewer 70S ribosomes in cells. No effect on sporulation or the general stress response.
No effect on growth or ribosomes, eliminates sporulation onset. Also decreases the general stress response to physical stress.
Stops growing at 45 degrees Celsius, shows sporulation onset defects. KM for GTP is 2.3 uM, turnover number is 0.015/min.
Cystathionine gamma-synthase/O-acetylhomoserine (thiol)-lyase
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Phosphoenolpyruvate-protein phosphotransferase
Putative ribosomal N-acetyltransferase YdaF
Putative HTH-type transcriptional regulator YvaV
Uncharacterized HTH-type transcriptional regulator YqaE
SPbeta prophage-derived uncharacterized protein YomQ
Uncharacterized HTH-type transcriptional regulator YvnA
17-fold increased production of c-di-AMP in E.coli.
20-fold increased production of c-di-AMP in E.coli, forms only homodimers.
Suppresses a triple DAC mutant, 10-fold increased production of c-di-AMP in E.coli, forms hexamers.
Suppresses a triple DAC mutant, 12-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 20-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 4-fold increased production of c-di-AMP in E.coli.
Suppresses a triple DAC mutant, 90-fold increased production of c-di-AMP in E.coli.
Manganese transport system membrane protein MntC
1-acyl-sn-glycerol-3-phosphate acyltransferase
SPbeta prophage-derived uncharacterized protein YopU
Carbamoyl-phosphate synthase pyrimidine-specific small chain
No effect on cell shape or cell viability.
Glutamate-1-semialdehyde 2,1-aminomutase 2
Cytochrome c-type biogenesis protein CcdA
Alkaline phosphatase synthesis transcriptional regulatory protein PhoP
SPbeta prophage-derived uncharacterized protein YopK
Oligopeptide transport ATP-binding protein OppF
Probable mannose-6-phosphate isomerase GmuF
Cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB
Glutathione/L-cysteine transport system ATP-binding/permease protein CydD
Peptide methionine sulfoxide reductase MsrA
Uncharacterized zinc-type alcohol dehydrogenase-like protein YogA
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
SPbeta prophage-derived uncharacterized protein YomH
Increases the stability of phosphorylated form.
Lack of phosphorylation. Decreases degradative enzyme production, but allows full expression of late competence genes.
Prevents both swarming motility and pellicle formation.
SPbeta prophage-derived uncharacterized protein YonC
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta
Putative UTP--glucose-1-phosphate uridylyltransferase
Single-stranded-DNA-specific exonuclease RecJ
Bifunctional oligoribonuclease and PAP phosphatase NrnA
Probable membrane transporter protein YunE
Restores reduced sporulation efficiency in a phrA deletion mutant.
Retains partial activity toward RapA and shows activity toward RapB.
Uncharacterized N-acetyltransferase YoaA
Probable spore germination lipoprotein YhcN
Probable formaldehyde dehydrogenase AdhA
Putative ribosomal RNA large subunit methyltransferase YwbD
Does not affect binding to FlhF, but abolishes its activation.
Probable NADH-dependent flavin oxidoreductase YqiG
SPbeta prophage-derived uncharacterized protein YopA
Sec-independent protein translocase protein TatAy
SPbeta prophage-derived uncharacterized protein YosC
Putative teichuronic acid biosynthesis glycosyltransferase TuaG
DNA-directed RNA polymerase subunit delta
Glyceraldehyde-3-phosphate dehydrogenase 1
Probable HTH-type transcriptional regulator YttP
Asparagine synthetase [glutamine-hydrolyzing] 2
Loss of cooperativity with ATP. 5-fold decrease in affinity for UMP. Increased sensitivity to activation by GTP.
Loss of cooperativity with ATP. Increased sensitivity to activation by GTP.
L-cystine transport system permease protein TcyM
Iron-uptake system permease protein FeuB
Probable xanthine dehydrogenase subunit D
ABC transporter ATP-binding protein YtrB
Forms flat, unwrinkled biofilm. Biofilm retains nonwetting, hydrophobic nature.
Forms flat, unwrinkled biofilm. Loss of colony hydrophobicity.
Partial loss of morphological complexity. Biofilm retains nonwetting, hydrophobic nature.
Abolishes ATP-binding and DNA translocation.
In SpoIIIE-73-11; defects in DNA translocation. Can complete engulfment and membrane fusion.
Putative teichuronic acid biosynthesis glycosyltransferase TuaC
UDP-N-acetylenolpyruvoylglucosamine reductase
SPbeta prophage-derived uncharacterized protein YonK
N5-carboxyaminoimidazole ribonucleotide mutase
Uncharacterized ABC transporter ATP-binding protein YvfR
Glycine betaine transport system permease protein OpuAB
SPbeta prophage-derived uncharacterized protein YolD
Probable ABC transporter permease protein YurN
Probable amino-acid ABC transporter permease protein YckA
Uncharacterized isochorismatase family protein YaaI
Probable nitrate reductase molybdenum cofactor assembly chaperone NarJ
In gutR1; results in the constitutive expression of gutB.
Teichoic acid translocation permease protein TagG
Uncharacterized N-acetyltransferase YfmK
Uncharacterized HTH-type transcriptional regulator YvbU
SPbeta prophage-derived uncharacterized protein YotI
Putative serine/threonine-protein kinase YrzF
Bifunctional purine biosynthesis protein PurH
Decrease in induction of sigma-B activity in response to a salt stress. In absence of the other RsbR paralogs no change in stress response when RsbRB.
Decrease in induction of sigma-B activity in response to a salt stress. In absence of the other RsbR paralogs no change in stress response.
No phosphorylation; when associated with A-171. In absence of the other RsbR paralogs unable to function as a co-antagonist when RsbRB.
No phosphorylation; when associated with A-205. No stress response. In absence of the other RsbR paralogs greatly reduced stress response.
Affects negative regulation of the antitermination activity.
Affects negative regulation of the antiterminator activity. No effect on phosphorylation by HPr. Abolishes phosphorylation by both HPr and EII-Glc; when associated with D-211.
Affects negative regulation of the antiterminator activity; when associated with S-102.
Affects negative regulation of the antiterminator activity; when associated with T-103.
Drastically reduced antiterminator activity.
Drastically reduced antiterminator activity; when associated with N-38.
Drastically reduced antiterminator activity; when associated with R-9.
Loss of antiterminator activity; when associated with L-5.
Loss of antiterminator activity; when associated with P-19.
Loss of antiterminator activity; when associated with S-5.
Loss of antiterminator activity; when associated with T-29.
No effect on negative regulation of the antiterminator activity.
Strongly reduced phosphorylation by HPr. Abolishes phosphorylation by both HPr and EII-Glc; when associated with A-104 or D-163.
Uncharacterized N-acetyltransferase YoaP
Probable spore germination protein GerPF
SPbeta prophage-derived endonuclease YokF
Probable NADH-dependent butanol dehydrogenase 2
Putative phosphotransferase enzyme IIA component YyzE
Uncharacterized HTH-type transcriptional regulator YqaG
Antilisterial bacteriocin subtilosin biosynthesis protein AlbA
No longer cleaves SAM, does not make thioether bonds, contains 68% iron of wild-type.
Weakly cleaves SAM but does not make thioether bonds, contains 68% iron of wild-type.
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Probable succinyl-CoA:3-ketoacid coenzyme A transferase subunit B
Uncharacterized ABC transporter ATP-binding protein YxlF
Almost loss of activity. Decreases tRNA binding.
Cannot form a dimer and has no activity; when associated with Glu-253.
Cannot form a dimer and has no activity; when associated with Glu-256.
Strong decrease in activity. Decreases tRNA binding.
Uncharacterized amino acid permease YbgF
Uncharacterized HTH-type transcriptional regulator YdcH
Probable polyketide biosynthesis enoyl-CoA hydratase PksH
Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
Zinc-specific metallo-regulatory protein
SPbeta prophage-derived uncharacterized protein YotD
Glycine betaine/carnitine/choline-binding protein OpuCC
Uncharacterized transcriptional regulatory protein YrkP
Ribosomal RNA small subunit methyltransferase A
Gly-cisPro motif, important for rejection of L-amino acids
Putative glycerophosphodiester phosphodiesterase YhdW
Uncharacterized EAL-domain containing protein YkuI
SPbeta prophage-derived uncharacterized protein YosF
Probable spore germination protein GerPA
Uncharacterized HTH-type transcriptional regulator YcbG
Bacteria grow as well as wild-type, are slightly longer, protein no longer binds bocillin-FL (a penicillin analog). Increased sensitivity to cefoxitin, very slight increase in resistance to penicillin G and cephalexin.
Cells are viable and motile, however they are filamentous and only about 20% of cells produce spores. Less protein is made, still binds penicillin.
Putative peptide biosynthesis protein YydG
2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Purine nucleoside phosphorylase DeoD-type
Uncharacterized MFS-type transporter YdeG
Uncharacterized transcriptional regulatory protein YbdJ
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheH
Acetoin dehydrogenase operon transcriptional activator AcoR
Assimilatory nitrite reductase [NAD(P)H] small subunit
Broad specificity amino-acid racemase RacX
DNA-binding transcriptional activator AdeR
Uncharacterized HTH-type transcriptional regulator YisR
Electron transfer flavoprotein subunit alpha
Interaction with polymerase core subunit RpoC
Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagV
SPbeta prophage-derived ribonucleoside-diphosphate reductase subunit beta
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Uncharacterized HTH-type transcriptional regulator YxaF
Uncharacterized glycosyltransferase YkcC
Spore coat polysaccharide biosynthesis protein SpsL
Short-chain dehydrogenase/reductase homolog YusS
SPbeta prophage-derived uncharacterized protein YorV
D-alanyl-D-alanine carboxypeptidase DacF
Glycerol phosphate lipoteichoic acid synthase 2
Processed glycerol phosphate lipoteichoic acid synthase 2
Putative methyl-accepting chemotaxis protein YoaH
Accumulation of S-bacillithiolated form due to lack of this resolving cysteine residue. Debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) without the prior NaBH(4) reduction of this protein in vitro. Debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Retains isomerization activity of the disulfide bonds in scrambled tachyplesin substrate. Loss of isomerization activity; when associated with A-53.
Loss of debacillithiolation of the S-bacillithiolated OhrR (OhrR-SSB) by the NaBH(4)-reduced form of this protein in vitro. Loss of debacillithiolation of in vivo NaOCl-generated S-bacillithiolated MetE (MetE-SSB). Retains isomerization activity of the disulfide bonds in scrambled tachyplesin substrate. Loss of isomerization activity; when associated with A-55.
Uncharacterized ABC transporter ATP-binding protein YwjA
Uncharacterized amino acid permease YvbW
UTP--glucose-1-phosphate uridylyltransferase
Probable coenzyme A transferase subunit alpha
Uncharacterized HTH-type transcriptional regulator YdeP
Bifunctional muramidase/DL-endopeptidase CwlT
Loss of muramidase activity. Shows no detectable transfer of ICEBs1.
Loss of peptidase activity. 1000-fold decrease in conjugation efficiency.
No detectable change in conjugation efficiency.
PTS system maltose-specific EIICB component
Nucleophile; cysteine thiosulfonate intermediate
Uncharacterized transcriptional regulatory protein YhcZ
Uncharacterized ABC transporter ATP-binding protein YdbJ
Does not affect secretion and dimerization.
Lack of secretion, but does not affect dimerization.
Putative polysaccharide deacetylase YxkH
Uncharacterized HTH-type transcriptional regulator YdfF
Destroys both polymerase and exonuclease activities.
Destroys exonuclease activity; decreases polymerase activity.
Putative sodium/proton-dependent alanine carrier protein YrbD
N-acetyl-alpha-D-glucosaminyl L-malate synthase
Uncharacterized HTH-type transcriptional regulator YbbH
Loss of enzyme activity and N-terminal processing.
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase 1
DNA-directed RNA polymerase subunit omega
18% of wild-type activity, but 100-fold decrease in substrate affinity.
50% of wild-type activity, but 9-fold decrease in substrate affinity.
8% of wild-type activity, but 7-fold decrease in substrate affinity.
Complete loss of activity, and widespread change in 3D-structure.
Glycerol-3-phosphate cytidylyltransferase
Reduces affinity for glycerol 3-phosphate about 100-fold.
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YotL
UPF0735 ACT domain-containing protein YszB
Menaquinol-cytochrome c reductase iron-sulfur subunit
Uncharacterized HTH-type transcriptional regulator YbfI
Uncharacterized ABC transporter ATP-binding protein YknV
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Branched-chain amino acid transport protein AzlC
Putative peptidyl-prolyl cis-trans isomerase YacD
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
Polyribonucleotide nucleotidyltransferase
Bifunctional muramidase/lytic transglycosylase CwlQ
Lacks both muramidase and transglycosylase activities.
Lacks both muramidase and transglycosylase activities. Confers a defect in swarming motility.
Glutamate synthase large subunit-like protein YerD
Uncharacterized MscS family protein YhdY
Uncharacterized HTH-type transcriptional regulator YwqM
Loss of most ability to inhibit sigma-G, no longer interacts with sigma-G, replace sequence with same region from C.acetobutylicum.
Loss of most sigma-G inhibition, still slight interaction with sigma-G.
No longer inhibits or interacts with sigma-G.
No longer inhibits sigma-G, still slight interaction with sigma-G.
No longer inhibits sigma-G. Restores 70% of sigma-G inhibition; when associated with 11-A--A-14.
No longer inhibits sigma-G. Restores 70% of sigma-G inhibition; when associated with 30-A--A-33.
Response regulator aspartate phosphatase I
Prevents a complete closure of the inter-domain cleft, affecting ATP binding, ATP hydrolysis and RNA unwinding.
Slows down the catalytic cycle, but does not affect formation of a closed conformer and global conformation; when associated with A-182.
Slows down the catalytic cycle, but does not affect formation of a closed conformer and global conformation; when associated with A-184.
Still adopts a closed conformation upon binding of ATP and RNA, but lacks ATPase and RNA unwinding activities.
Methyl-accepting chemotaxis protein McpC
Uncharacterized N-acetyltransferase YsnE
SPbeta prophage-derived uncharacterized protein YotB
CDP-diacylglycerol--serine O-phosphatidyltransferase
50% wild-type amount of Cys-thioether cross-link made.
Approximately wild-type amounts of Cys-thioether cross-link made.
Cyclic, disulfide-containing sactipeptide with a thioether cross-link of cysteine to methionine.
Cys-Met cross-link not made, suggests relative amino acid positions are important for thioether bond formation.
5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YitU
Uncharacterized ABC transporter ATP-binding protein YkpA
Manganese transport system membrane protein MntD
Ribonucleoside-diphosphate reductase NrdEB subunit alpha, 1st part
Ribonucleoside-diphosphate reductase NrdEB subunit alpha, 2nd part
130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.
2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Probable capsular polysaccharide biosynthesis protein YwqC
Uncharacterized MFS-type transporter YitZ
Uncharacterized metallophosphoesterase YkuE
scyllo-inositol 2-dehydrogenase (NAD(+))
Uncharacterized ABC transporter ATP-binding protein YthP
Induction of srfA is delayed approximately 1 generation.
Induction of srfA is delayed by 2 generations or more.
Uncharacterized N-acetyltransferase YycN
Succinate dehydrogenase iron-sulfur subunit
Putative competence-damage inducible protein
Loss of activity. Cannot complement the null mutant.
Reduces the farnesylation activity using FPP as the prenyl donor, but geranylation activity using GPP is comparable to that of the wild-type enzyme.
Uncharacterized MFS-type transporter YdeR
Succinate--CoA ligase [ADP-forming] subunit beta
Imidazole glycerol phosphate synthase subunit HisF
30% endonuclease and 10% 5'-exonuclease activity.
Loss of endo- and 5'-exonuclease activity.
Severe loss of endo- and 5'-exonuclease activity.
Slight decrease in endonuclease and 10-fold decrease in 5'-exonuclease activity; when associated with A-337.
Slight decrease in endonuclease and 10-fold decrease in 5'-exonuclease activity; when associated with A-45.
Uncharacterized ABC transporter substrate-binding lipoprotein YvrC
Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.
Polyketide biosynthesis cytochrome P450 PksS
Phenylalanine--tRNA ligase alpha subunit
Uncharacterized glycosyltransferase EpsJ
Large-conductance mechanosensitive channel
Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
Unsaturated rhamnogalacturonyl hydrolase YteR
Uncharacterized MFS-type transporter YusP
Alpha-ketoglutaric semialdehyde dehydrogenase
Uncharacterized transcriptional regulatory protein YclJ
Phosphoribosylglycinamide formyltransferase
Loss of catalytic activity. Unable to form an octanoyl-enzyme intermediate.
Octanoyl-[GcvH]:protein N-octanoyltransferase
Probable molybdenum cofactor guanylyltransferase
PTS system beta-glucoside-specific EIIBCA component
1.5- and 2-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
5- and 2-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
5.5- and 3-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
6- and 3-fold decrease of the catalytic efficiency and the affinity for ex-H2HPP, respectively.
In sboA1; protein has acquired hemolytic activity and is more effective against tested Gram-positive bacteria.
In vitro can no longer make the Cys-thioether cross-link, variable loss of the other 2 thioether cross-links, in vivo no cyclopeptide formed.
In vitro no longer makes the first thioether cross-link, in vivo no cyclopeptide formed.
In vitro no longer makes the second thioether cross-link, in vivo no cyclopeptide formed.
In vivo forms 2 thioether cross-links, no cyclopeptide formed.
In vivo forms 3 thioether cross-links, forms the cyclopeptide.
Uncharacterized HTH-type transcriptional regulator YkvN
Putative [ribosomal protein S5]-alanine N-acetyltransferase
SPbeta prophage-derived uncharacterized protein YomT
Probable N-acetyl-alpha-D-glucosaminyl L-malate deacetylase 2
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
SPbeta prophage-derived uncharacterized HTH-type transcriptional regulator YopS
Maltodextrin transport system permease protein MdxF
Oligopeptide transport system permease protein AppB
Causes mislocalization and strongly impairs sporulation. Does not affect membrane association.
Does not affect localization or sporulation.
Slightly affects localization and sporulation.
Specifically impairs cortex assembly, but not the initiation of coat assembly. Strongly impairs sporulation, but does not affect localization.
Strongly impairs sporulation, but does not affect localization.
Probable glycolate oxidase iron-sulfur subunit
SPbeta prophage-derived uncharacterized protein YorY
PTS system fructose-specific EIIA component
Phosphorylation is only slightly increased.
Uncharacterized ABC transporter ATP-binding protein YcbN
Putative peptide zinc metalloprotease protein YydH
SPbeta prophage-derived uncharacterized protein YotE
Does not complement an ftsH deletion, loss of ATPase activity.
Does not complement an ftsH deletion, loss of protease activity against casein.
Binds potassium in the micromolar range.
Uncharacterized ABC transporter ATP-binding protein YknY
Uncharacterized N-acetyltransferase YitI
Probable polyketide biosynthesis zinc-dependent hydrolase PksB
Uncharacterized HTH-type transcriptional regulator YkvZ
3-isopropylmalate dehydratase large subunit
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
Uncharacterized HTH-type transcriptional regulator YcnC
Uncharacterized HTH-type transcriptional regulator YdfL
Putative molybdenum transport system permease protein YvgM
Charge relay system; for autoendoproteolytic cleavage activity
Phosphatidylserine decarboxylase alpha chain
Phosphatidylserine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity
HTH-type transcriptional regulatory protein GabR
Uncharacterized HTH-type transcriptional regulator YfiR
Uncharacterized ABC transporter ATP-binding protein YvcR
Uncharacterized HTH-type transcriptional regulator YcxD
3-methyl-2-oxobutanoate hydroxymethyltransferase
Spore coat polysaccharide biosynthesis protein SpsC
Uncharacterized HTH-type transcriptional regulator YybR
SPbeta prophage-derived uncharacterized protein YorO
Antilisterial bacteriocin subtilosin biosynthesis protein AlbE
SPbeta prophage-derived uncharacterized protein YomK
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Organic hydroperoxide resistance protein OhrB
SPbeta prophage-derived uncharacterized protein YopN
SPbeta prophage-derived uncharacterized protein YoqU
Probable L-serine dehydratase, alpha chain
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
SPbeta prophage-derived deoxyuridine 5'-triphosphate nucleotidohydrolase YosS
Branched-chain-amino-acid transaminase 1
25% retention of activity. Two to three fold reduction in KM for acetyl-CoA. Three-fold slower turnover number. Retains 60% of the activity of the wild-type after heating it to 100 degrees Celsius for 30 minutes.
25% retention of activity. Two to three-fold reduction in KM for acetyl-CoA. Three-fold slower turnover number. Retains 54% of the activity of the wild-type after heating it to 100 degrees Celsius for 30 minutes.
Inactive. Leads to a rapid turnover of the enzyme.
Inhibits autoproteolysis of protein and spore formation.
Reduced binding to PDZ domain and sporulation deficient spores.
Putative gamma-glutamylcyclotransferase YkqA
30-fold decrease in activity but no decrease in the overall phosphorylation level.
Putative fluoride ion transporter CrcB 2
SPbeta prophage-derived uncharacterized protein YorE
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Uncharacterized FAD-linked oxidoreductase YgaK
Uncharacterized PIN and TRAM-domain containing protein YacL
Uncharacterized HTH-type transcriptional regulator YsmB
3- to 5-fold weaker affinity for PDZ ligands and reduced proteolytic activity against pre-processed SpoIVFA substrate. Less than residual protease activity; when associated with Y-118.
Carboxy-terminal processing protease CtpB
Constitutively active protease with higher activity than wild-type protease and total loss of substrate specificity.
Loss of peptide binding to the PDZ domain, but still has residual protease activity. Less than residual protease activity; when associated with A/F-168.
No cleavage by SpoIVB. No effect on pro-sigma-K processing.
Heptaprenyl diphosphate synthase component 2
SPbeta prophage-derived UPF0715 membrane protein YopD
Uncharacterized SPbeta prophage-derived protein YoqJ
Loss of activity in vivo, about 100-fold loss in vitro.
Loss of activity, significant loss of rRNA binding.
Uncharacterized HTH-type transcriptional regulator YdeF
Cannot fulfill its catalytic role within PTS transport. Does not affect dimerization.
PTS system fructose-specific EIIABC component
Phosphoribosylformylglycinamidine synthase subunit PurL
SPbeta prophage-derived uncharacterized protein YosG
SPbeta prophage-derived uncharacterized protein YomR
ATP-dependent protease ATPase subunit ClpY
Loss of methylase activity, still binds DNA.
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex
Dipeptide transport system permease protein DppB
Probable multidrug resistance ABC transporter ATP-binding/permease protein YheI
SPbeta prophage-derived uncharacterized protein YolB
Intracellular exo-alpha-L-arabinofuranosidase 2
SPbeta prophage-derived uncharacterized protein YonP
2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
Alkaline phosphatase synthesis sensor protein PhoR
Arginine transport ATP-binding protein ArtM
In dnaD23; temperature-sensitive mutant that affects the initiation of chromosome replication.
Almost no effect on catalytic efficiency.
Hydrolyzes ATP, even in the absence of L-Ala, and the structure appears to show a cavity in the N-terminal substrate-binding pocket. Also alters the substrate specificity and activity depending on the size and shape of substituted amino acids.
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
Putative NAD(P)H-dependent FMN-containing oxidoreductase YwqN
SPbeta prophage-derived uncharacterized protein YoqP
SPbeta prophage-derived uncharacterized protein YorW
Petrobactin import ATP-binding protein YclP
Putative metabolite transport protein YyaJ
Putative sigma L-dependent transcriptional regulator YqiR
Great decrease in binding affinity for mRNA.
Increased ability to bind to hut mRNA in the presence of histidine.
Slight decrease in binding affinity for mRNA.
Grows slowly, cells have abnormal morphology.
Higher endogenous GTPase activity, GTPase more stimulated by 70S ribosomes than wild-type, increased association with 30S ribosomal subunit in vitro, wild-type behavior in vivo.
Loss of most phosphorylation by PrkC, GTPase activity less stimulated by 70S ribosomes than wild-type, decreased association with 30S ribosomal subunit in vitro. Grows slowly, cells have abnormal morphology.
Loss of phosphorylation by PrkC, but PrkC autophosphorylation considerably decreased in vitro.
Loss of phosphorylation by PrkC, but PrkC autophosphorylation greatly decreased in vitro.
No GTPase activity. Wild-type phosphorylation by PrkC in vitro.
Small ribosomal subunit biogenesis GTPase RsgA
Wild-type phosphorylation by PrkC in vitro.
Binds c-di-AMP, but interaction with RelA is weakened and not affected by c-di-AMP; when associated with G-25.
Binds c-di-AMP, but interaction with RelA is weakened and not affected by c-di-AMP; when associated with M-132.
Binds c-di-AMP. Abolishes the ability to bind RelA. Is still able to bind PYC, but cannot activate it.
Uncharacterized glycosyltransferase YdaM
Putative ABC transporter substrate-binding protein YesO
SPbeta prophage-derived recombinase-like protein YomM
SPbeta prophage-derived uncharacterized protein YonD
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabL
Uncharacterized acyl-CoA thioester hydrolase YkhA
Spore coat polysaccharide biosynthesis protein SpsK
Glutamyl-tRNA(Gln) amidotransferase subunit A
Oligopeptide transport ATP-binding protein OppD
SPbeta prophage-derived uncharacterized protein YoqF
Uncharacterized metallophosphoesterase YunD
Sec-independent protein translocase protein TatCd
Uncharacterized HTH-type transcriptional regulator YdgC
Probable spore germination lipoprotein YlaJ
Probable glucosamine-6-phosphate deaminase 2
Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-227.
Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-228.
Prevents phosphorylation and increases ATPase activity; when associated with F-227 and F-228.
SPbeta prophage-derived uncharacterized protein YopV
Spore coat polysaccharide biosynthesis protein SpsF
Putative Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YjdI
Probable rhamnogalacturonan acetylesterase YesY
Branched-chain amino acid transport protein AzlD
SPbeta prophage-derived uncharacterized protein YomD
Putative HTH-type transcriptional regulator YwnA
Iron-uptake system permease protein FeuC
Uncharacterized ABC transporter permease protein YvrB
Probable xanthine dehydrogenase subunit E
Extracellular endo-alpha-(1->5)-L-arabinanase 1
Significantly less active (10000-fold) than the wild-type arabinanase against both linear arabinan and arabinooligosaccharides.
Significantly less active than the wild-type arabinanase against both linear arabinan (50-fold) and the arabinooligosaccharides (1000-fold).
SPbeta prophage-derived uncharacterized protein YorH
Uncharacterized ATP-dependent helicase YqhH
Probable 5-dehydro-4-deoxyglucarate dehydratase
Probable siderophore transport system permease protein YfhA
SPbeta prophage-derived uncharacterized protein YopL
Dipeptide transport ATP-binding protein DppD
Uncharacterized MFS-type transporter YcxA
Putative aspartate aminotransferase YhdR
In BPSC 31 and BPSC 61; spectinomycin resistant.
In rpsE1; spectinomycin restistant, not a ram mutant.
In rpsE7; suppresses S12 mutant K55D. A ram mutation.
In rpsE8; suppresses S12 mutant K55D. RspE8 is lethal in the absence of the S12 mutation.
In rpsE9; suppresses S12 mutation K55D. Lethal in the absence of the S12 mutation.
D-alanyl-D-alanine carboxypeptidase DacB
Putative HMP/thiamine permease protein YkoE
Uncharacterized HTH-type transcriptional regulator YvkB
Probable peptidoglycan glycosyltransferase FtsW
3-phosphoshikimate 1-carboxyvinyltransferase
Uncharacterized sodium-dependent transporter YocS
Uncharacterized ABC transporter ATP-binding protein YvrO
TVP38/TMEM64 family membrane protein YtxB
SPbeta prophage-derived uncharacterized protein YoqX
Uncharacterized HTH-type transcriptional regulator YobD
SPbeta prophage-derived uncharacterized protein YomU
ABC transporter ATP-binding protein YxdL
Uncharacterized metallophosphoesterase YkoQ
Probable ABC transporter permease protein YurM
Asparagine synthetase [glutamine-hydrolyzing] 1
Uncharacterized HTH-type transcriptional regulator YybA
Probable dual-specificity RNA methyltransferase RlmN
Probable C4-dicarboxylate response regulator DctR
SPbeta prophage-derived pesticidal crystal protein-like YokG
Negative regulator of genetic competence ClpC/MecB
Choline transport system permease protein OpuBD
Putative ABC transporter substrate-binding lipoprotein YvgL
Decrease in phosphatase activity in vitro. Displays reduced activity in vivo.
Displays drastically reduced activity in vivo.
Loss of phosphatase activity in vitro. Displays reduced activity in vivo.
No change in phosphatase activity in vitro.
Response regulator aspartate phosphatase H
Flagellar hook-basal body complex protein FlhO
Trifunctional nucleotide phosphoesterase protein YfkN
SPbeta prophage-derived uncharacterized protein YoqM
Probable glutamine ABC transporter permease protein GlnP
Polygalacturonan/rhamnogalacturonan transport system permease protein YtcP
Uncharacterized HTH-type transcriptional regulator YbzH
Uncharacterized HTH-type transcriptional regulator YwaE
SPbeta prophage-derived uncharacterized protein YomL
SPbeta prophage-derived uncharacterized protein YonG
Loss of cysteine desulfurase activity, still binds SufU and Cys.
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
Probable L-serine dehydratase, beta chain
PTS system oligo-beta-mannoside-specific EIIC component
Probable spore germination protein GerPB
Putative Rieske 2Fe-2S iron-sulfur protein YhfW
Putative TrmH family tRNA/rRNA methyltransferase YacO
Glycine betaine/carnitine/choline transport system permease protein OpuCD
Probable iron uptake system component EfeM
Uncharacterized N-acetyltransferase YdfB
Probable guanidinium efflux system subunit GdnD
Uncharacterized ABC transporter ATP-binding protein YhaQ