Phenylalanine--tRNA ligase alpha subunit
Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 2
Adenosylcobinamide-GDP ribazoletransferase
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase
Molybdate/tungstate transport system permease protein WtpB
Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr), no activity on L-threonyl-tRNA(Thr).
Carbamoyl-phosphate synthase large chain
DNA-directed RNA polymerase subunit Rpo11
Probable ribosomal RNA small subunit methyltransferase A
2.4-fold decrease in substrate affinity.
95% reduction in activity compared to wild type.
Nucleophile; O-isoaspartyl threonine intermediate
Shows improved enzymatic properties at physiological conditions (pH 7.4 and 37 degrees Celsius) as compared to the wild type with a 2-fold increase in catalytic efficiency. Shows higher and significant killing of human leukemic and breast carcinoma cell lines as compared to the E.coli L-asparaginase.
Shows improved enzymatic properties at physiological conditions (pH 7.4 and 37 degrees Celsius) as compared to the wild type with a 2.6-fold increase in catalytic efficiency. Shows higher and significant killing of human leukemic and breast carcinoma cell lines as compared to the E.coli L-asparaginase.
Probable cobalamin biosynthesis protein CobD
DNA double-strand break repair Rad50 ATPase
Decreases ATP-induced dimerization. Increases DNA end tethering.
Destabilizes the ATP-dimerized state. Promotes HerA/NurA activity.
Increases ATP-induced dimerization. Increases DNA end tethering. Fails to promote HerA/NurA activity.
Prevents ATP binding and disrupts the communication among the other ATP-binding loops. Prevents also Rad50 dimerization. Decreases both ATPase and adenylate kinase activities.
Putative HTH-type transcriptional regulatory protein PF1851
No homotrimer formation. Stimulates ATPase activity of RFC, no stimulation of DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers.
Small amount of homotrimer in solution. Stimulates ATPase activity of RFC and DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers.
Schiff-base intermediate with dihydroxyacetone-P
Probable glycine dehydrogenase (decarboxylating) subunit 1
Putative CRISPR-associated endoribonuclease-like protein Cas6
Glyceraldehyde-3-phosphate dehydrogenase
Putative 1-aminocyclopropane-1-carboxylate deaminase
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with D-211, D-213 and A-223.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211 and A-223.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-211, and D-213.
Decreases catalytic activity for insosine and allows the enzyme to phosphorolytically cleave MTA with a catalytic efficiency about 4-fold higher than for inosine; when associated with S-169, D-213 and A-223.
Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-254.
Fully active, but reduces thermodynamic and kinetic stability of the enzyme; when associated with S-256.
Probable 6-oxopurine nucleoside phosphorylase
Reduces enzymatic activity by 96% at 37 degrees Celsius and by 88% at 87 degrees Celsius; when associated with A-161.
Reduces enzymatic activity by 96% at 37 degrees Celsius and by 88% at 87 degrees Celsius; when associated with A-173.
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
tRNA N6-adenosine threonylcarbamoyltransferase
N-(5'-phosphoribosyl)anthranilate isomerase
Nicotinamide-nucleotide adenylyltransferase
Nascent polypeptide-associated complex protein
UPF0173 metal-dependent hydrolase PF1764
5-methylthioadenosine/S-adenosylhomocysteine deaminase
Formate-dependent phosphoribosylglycinamide formyltransferase
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Tungsten-containing aldehyde ferredoxin oxidoreductase
tRNA (uracil(54)-C(5))-methyltransferase
DNA double-strand break repair helicase HerA
Putative [LysW]-lysine/[LysW]-ornithine hydrolase
Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit
GMP synthase [glutamine-hydrolyzing] subunit A
Glutamyl-tRNA(Gln) amidotransferase subunit E
DNA repair and recombination protein RadA
3-isopropylmalate dehydratase large subunit 2
Becomes active at low temperatures; when associated with C-228 or D-241.
Becomes active at low temperatures; when associated with G-278.
Increased dissociation of dodecamers into trimers.
Ornithine carbamoyltransferase, anabolic
Membrane-bound hydrogenase subunit alpha
Putative HTH-type transcriptional regulator TrmBL2
Molybdate/tungstate import ATP-binding protein WtpC
Phosphoribosylformylglycinamidine synthase subunit PurL
Signal recognition particle 19 kDa protein
Removed in mature form; by autocatalysis
Uncharacterized HTH-type transcriptional regulator PF0054
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Pyruvoyl-dependent arginine decarboxylase subunit beta
Pyruvate/ketoisovalerate oxidoreductases common subunit gamma
Probable translation initiation factor IF-2, 1st part
Probable translation initiation factor IF-2, 2nd part
3-isopropylmalate dehydratase large subunit 1
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase alpha chain
S-adenosylmethionine decarboxylase beta chain
Schiff-base intermediate with substrate; via pyruvic acid
DNA protection during starvation protein
Resolvase/invertase-type recombinase catalytic
Histidine biosynthesis bifunctional protein HisIE
Imidazole glycerol phosphate synthase subunit HisF
10% HJ cleavage, binds HJ DNA about 50%.
20-fold decrease in HJ cleavage, decreased binding to HJ DNA.
No HJ cleavage. Slight decrease in DNA binding.
No HJ cleavage; at very high levels altered cleavage patterns. Dimerizes poorly.
No Holliday junction (HJ) cleavage, does not bind HJ DNA.
Wild-type HJ cleavage. Slight decrease in DNA binding.
Ketoisovalerate oxidoreductase subunit VorB
Aspartate carbamoyltransferase regulatory chain
Methylated-DNA--protein-cysteine methyltransferase
Acetate--CoA ligase [ADP-forming] I subunit beta
Reduced ATP binding and enzyme activity; when associated with A-531.
Reduced ATP binding and enzyme activity; when associated with A-534.
Polyribonucleotide 5'-hydroxyl-kinase PF0112
Phosphoribosylaminoimidazole-succinocarboxamide synthase
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Uncharacterized HTH-type transcriptional regulator PF0250
Translation initiation factor 2 subunit beta
Proton acceptor; for FBP phosphatase activity
Proton donor/acceptor; for FBP aldolase activity
Schiff-base intermediate with DHAP; for FBP aldolase activity
Pyridoxal 5'-phosphate synthase subunit PdxT
Dolichyl-phosphooligosaccharide-protein glycotransferase 2
Putative transcriptional regulatory protein PF0535
Ketoisovalerate oxidoreductase subunit VorA
Sugar fermentation stimulation protein homolog
O-(5'-phospho-DNA)-tyrosine intermediate
Uncharacterized HTH-type transcriptional regulator PF2053
Acetate--CoA ligase [ADP-forming] I subunit alpha
Probable glycine dehydrogenase (decarboxylating) subunit 2
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Moderate decrease in cleavage of target RNA in the whole Cmr complex.
Nearly complete loss of cleavage of target RNA in the whole Cmr complex.
Significant decrease in cleavage of target RNA in the whole Cmr complex.
Carbamoyl-phosphate synthase small chain
Imidazole glycerol phosphate synthase subunit HisH
Probable cyclic pyranopterin monophosphate synthase
DNA-directed RNA polymerase subunit Rpo6
Putative [LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase
Putative type 4B encapsulin shell protein PF1875
DNA-directed RNA polymerase subunit Rpo12
2-iminobutanoate/2-iminopropanoate deaminase
Putative ABC transporter ATP-binding protein PF0528
DNA-directed RNA polymerase subunit Rpo5
V-type ATP synthase alpha chain, 1st part
V-type ATP synthase alpha chain, 2nd part
Probable molybdenum cofactor guanylyltransferase
Probable glycine cleavage system H protein
Phosphate import ATP-binding protein PstB
Involved in ionization of N3 of dUMP, leading to its activation
Ribosomal RNA small subunit methyltransferase Nep1
O-(3'-phospho-DNA)-tyrosine intermediate
Phosphoribosylformylglycinamidine cyclo-ligase
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 1st part
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 2nd part
Vitamin B12-dependent ribonucleoside-diphosphate reductase, 3rd part
Putative ABC transporter ATP-binding protein PF0068
Cysteine sulfenic acid (-SOH) intermediate
Interchain (with Cys-211); in linked form
Interchain (with Cys-46); in linked form
Acetate--CoA ligase [ADP-forming] II subunit beta
Glutamyl-tRNA(Gln) amidotransferase subunit D
DNA-directed RNA polymerase subunit Rpo3
NAD(P)H sulfur oxidoreductase (CoA-dependent)
Acetate--CoA ligase [ADP-forming] II subunit alpha
Glyceraldehyde-3-phosphate:ferredoxin oxidoreductase
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-831.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-831 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-796; A-831 and A-835.
Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-795; A-796; A-831 and A-835.
Replication factor C small subunit, 1st part
Replication factor C small subunit, 2nd part
Uncharacterized HTH-type transcriptional regulator PF1734
No longer complements secY24, an E.coli temperature-sensitive secY mutation.
Pyridoxal 5'-phosphate synthase subunit PdxS
Schiff-base intermediate with D-ribose 5-phosphate
3-methyl-2-oxobutanoate hydroxymethyltransferase
Putative methylthioribose-1-phosphate isomerase
Ketoisovalerate oxidoreductase subunit VorD
3-isopropylmalate dehydratase small subunit 2
Putative translation initiation factor eIF-2B subunit 2-like
tRNA (pseudouridine(54)-N(1))-methyltransferase
Digeranylgeranylglycerophospholipid reductase
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
DNA-directed RNA polymerase subunit Rpo10
Uncharacterized HTH-type transcriptional regulator PF1231
3-isopropylmalate dehydratase small subunit 1
Probable Brix domain-containing ribosomal biogenesis protein
Loss of interaction with Cmr2, no cleavage of target RNA when assembled in the Cmr complex.
Loss of interaction with Cmr2, still cleaves target RNA when assembled in the Cmr complex.
No cleavage of target RNA when assembled in the Cmr complex.
Still cleaves target RNA when assembled in the Cmr complex.
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Putative [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase
DNA-directed RNA polymerase subunit Rpo1C
Putative [LysW]-aminoadipate/[LysW]-glutamate kinase
No effect on the efficiency of electron transfer.
The efficiency of electron transfer is compromised.
The mixed spin cluster is converted into a homogeneous spin cluster (S=3/2).
ADP-dependent glucose/glucosamine kinase
Translation initiation factor 2 subunit alpha
23S rRNA (uracil(747)-C(5))-methyltransferase
Translation initiation factor 2 subunit gamma
tRNA (cytidine(56)-2'-O)-methyltransferase
Phosphoribosylformylglycinamidine synthase subunit PurQ
Uncharacterized HTH-type transcriptional regulator PF0864
Signal recognition particle 54 kDa protein
Digeranylgeranylglyceryl phosphate synthase
Probable membrane-bound hydrogenase subunit mbhJ
HTH-type sugar sensing transcriptional regulator TrmBL1
Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)