Allows binding to CUL1 and RBX1 and formation of an authentic SCF (SKP1-CUL1-F-box protein) complex.
26S proteasome non-ATPase regulatory subunit 7
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Loss of cell membrane recruitment following EGF stimulation.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Mitotic checkpoint serine/threonine-protein kinase BUB1
O-linked (Gal...) hydroxylysine; alternate
No effect on MAPK1/3 (ERK1/2) phosphorylation in response to IL18, with or without IL12. Substantial reduction in MAPK1/3 (ERK1/2) phosphorylation in response to IL18, with or without IL12; when associated with A-53 and A-58.
No effect on MAPK1/3 (ERK1/2) phosphorylation in response to IL18, with or without IL12. Substantial reduction in MAPK1/3 (ERK1/2) phosphorylation in response to IL18, with or without IL12; when associated with A-53 and A-71.
Substantial reduction in MAPK1/3 (ERK1/2) phosphorylation in response to IL18, with or without IL12.
Processed angiotensin-converting enzyme 2
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate
N6-(2-hydroxyisobutyryl)lysine; alternate
N6-(beta-hydroxybutyryl)lysine; alternate
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Increase in autophosphorylation activity.
Loss of CDC42-binding and impairment of autophosphorylation.
Loss of interaction with NEDD4 and drastic reduction in its ubiquitination.
Phosphotyrosine; by SRC and autocatalysis
FYVE, RhoGEF and PH domain-containing protein 2
Loss of early endosome localization; when associated with K-454.
Loss of early endosome localization; when associated with T-455.
No effect on early endosome localization and reduced JNK1 activation; when associated with A-287.
No effect on early endosome localization and reduced JNK1 activation; when associated with A-288.
Decreased 'Lys-48'-linked polyubiquitin binding.
Decreased 'Lys-48'-linked polyubiquitin binding. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-229 and Q-231.
Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with BAG6. Decreased association with the proteasome complex.
Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-201 and Q-229. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-205 and Q-229.
Decreased 'Lys-48'-linked polyubiquitin binding. Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-201 and Q-231. Loss of 'Lys-48'-linked polyubiquitin binding, loss of interaction with BAG6 and loss of association with the proteasome complex; when associated with Q-205 and Q-231.
Decreased 'Lys-48'-linked polyubiquitin binding; when associated with A-230.
Decreased 'Lys-48'-linked polyubiquitin binding; when associated with Q-227.
Loss of interaction with ubiquitin polymers and ubiquitinated proteins, loss of interaction with BAG6 and no effect on association with the proteasome complex; when associated with Q-229 and Q-231.
Loss of localization to the endoplasmic reticulum membrane. Loss of interaction with BAG6. No effect on association with the proteasome complex.
No effect on 'Lys-48'-linked polyubiquitin binding.
No effect on association with the proteasome complex; when associated with A-163 and A-173.
No effect on association with the proteasome complex; when associated with A-163 and A-187.
No effect on association with the proteasome complex; when associated with A-173 and A-187.
No effect on association with the proteasome complex; when associated with D-163 and D-173.
No effect on association with the proteasome complex; when associated with D-163 and D-187.
No effect on association with the proteasome complex; when associated with D-173 and D-187.
Coiled-coil domain-containing protein 160
Vacuolar protein sorting-associated protein 37B
In isoform 1, isoform 2, isoform 3, isoform 4 and isoform 5.
Integrin-linked kinase-associated serine/threonine phosphatase 2C
Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4D
Helical; Signal-anchor for type II membrane protein
Interchain (between light and heavy chains)
Glial cell line-derived neurotrophic factor
Induces an age-dependent neurodegenerative phenotype.
Ribosome quality control complex subunit NEMF
E3 ubiquitin-protein ligase pellino homolog 3
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate
Serine/threonine-protein kinase PRP4 homolog
Dimethyladenosine transferase 1, mitochondrial
Hematopoietically-expressed homeobox protein Hhex
Helical; Voltage-sensor; Name=Segment S4
Potassium voltage-gated channel subfamily G member 4
Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.
Loss of deubiquitinase activity and binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.
Ubiquitin carboxyl-terminal hydrolase 30
Tumor suppressor candidate gene 1 protein homolog
Guanylate cyclase soluble subunit alpha-1
RNA polymerase-associated protein CTR9 homolog
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Abolishes nuclear translocation. Prolongs Ca(2+) oscillations allowing them to occur during interphase.
Defective in Ca(2+) oscillation activity and loss of nuclear translocation.
Defective in Ca(2+) oscillation activity and shows slower nuclear translocation.
Loss of glycosaminoglycan chains; when associated with A-533.
Loss of glycosaminoglycan chains; when associated with A-544.
O-linked (Xyl...) (glycosaminoglycan) serine
Transforming growth factor beta receptor type 3
Single-pass membrane and coiled-coil domain-containing protein 4
Death domain-containing membrane protein NRADD
Helical; Signal-anchor for type III membrane protein
Aspartate 1-(chondroitin 4-sulfate)-ester
Inter-alpha-trypsin inhibitor heavy chain H2
5'-AMP-activated protein kinase subunit gamma-3
Neural proliferation differentiation and control protein 1
Loss of phosphorylation by PKA, constitutive MAPK binding.
Mimics phosphorylation by PKA, prevents MAPK binding.
Receptor-type tyrosine-protein phosphatase R
Cadherin EGF LAG seven-pass G-type receptor 1
Loss of lyso-PAFAT activity. LPCAT activity is partially reduced.
Lysophosphatidylcholine acyltransferase 1
No activity for acetyl-CoA. Activity for palmitoyl-CoA decreased.
No effect on activity for acetyl-CoA. Reduced activity for palmitoyl-CoA.
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Homeobox and leucine zipper protein Homez
Cullin-associated NEDD8-dissociated protein 2
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
IQ and AAA domain-containing protein 1-like
Sprouty-related, EVH1 domain-containing protein 2
Abolishes histone methyltransferase activity and subsequent repression. In GM3; does not form heterodimer with EHMT1 and is defective in mediating both H3K9me and DNA methylation.
Histone-lysine N-methyltransferase EHMT2
In GM4; defective in methyltransferase activity without affecting DNA methylation.
In GM6; does not form heterodimer with EHMT1 and is defective in mediating H3K9me.
In GM7; defective in methyltransferase activity without affecting DNA methylation; when associated with F-1207.
In GM7; defective in methyltransferase activity without affecting DNA methylation; when associated with V-1120.
N6,N6,N6-trimethyllysine; by EHMT2; alternate
N6,N6-dimethyllysine; by EHMT2; alternate
Strongly reduces histone methyltransferase activity.
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
In isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8.
In strain: 129/Sv; requires 2 nucleotide substitutions.
NACHT, LRR and PYD domains-containing protein 5
Short transient receptor potential channel 3
Abolishes phosphorylation and prevents phosphorylation of S-77.
Decreases phosphorylation and no effect on interaction with CDK7. Strongly reduces transcriptional activity.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Greatly reduced interaction with RXRA and NCOR1 and transcriptional activation.
No change in phosphorylation levels and no effect on transcriptional activity.
No effect on phosphorylation, no effect on transcriptional activity.
Reduced methylation levels. Little effect on interaction with RXRA or NCOR1. Small loss in transcriptional activation.
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4
Protein transport protein Sec61 subunit gamma
Disintegrin and metalloproteinase domain-containing protein 22
In isoform 10, isoform 11, isoform 12 and isoform 13.
In isoform 17, isoform 18, isoform 19 and isoform 20.
In isoform 2, isoform 5, isoform 8, isoform 15 and isoform 17.
Cytotoxic granule associated RNA binding protein TIA1
Hypoxia-inducible factor 1-alpha inhibitor
Rap1 GTPase-GDP dissociation stimulator 1
28S ribosomal protein S33, mitochondrial
Zinc finger HIT domain-containing protein 3
Probable ATP-dependent RNA helicase DDX6
Chromodomain-helicase-DNA-binding protein 4
Mice develop hair and nail defects resembling pachyonychia congenita.
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein
Ubiquitin domain-containing protein UBFD1
Leucine-rich repeat and transmembrane domain-containing protein 1
Reduces binding to phosphoinositide; when associated with Gln-109.
Reduces binding to phosphoinositide; when associated with Gln-33; Gln-34 and Gln-38.
Reduces binding to phosphoinositide; when associated with Gln-33; Gln-34 and Gln-42.
Reduces binding to phosphoinositide; when associated with Gln-33; Gln-38 and Gln-42.
Reduces binding to phosphoinositide; when associated with Gln-34; Gln-38 and Gln-42.
Reduces binding to phosphoinositide; when associated with Gln-93.
Tumor necrosis factor alpha-induced protein 8-like protein 3
Regulating synaptic membrane exocytosis protein 2
Cyclin-dependent kinases regulatory subunit 2
POU domain, class 4, transcription factor 2
Stimulates induction of neurite outgrowth and expression of synaptic genes. Abolishes the inhibitory effect on basal transcription and on gene activation by POU4F1.
Zinc finger C2HC domain-containing protein 1A
Signal-induced proliferation-associated 1-like protein 2
Class E basic helix-loop-helix protein 22
Activating molecule in BECN1-regulated autophagy protein 1
Abolishes localization to endosomes. Strongly affects inhibitory function. Abolishes inhibitory function; when associated with L-73.
Abolishes myristoylation and localization to endosomes.
Slightly affects inhibitory function. Abolishes inhibitory function; when associated with E-111.
Dynamin-like 120 kDa protein, mitochondrial
Leucine-rich repeat-containing protein 45
Abolishes sumoylation. No change in PIAS4 binding nor on transcriptional activity.
Disrupts interaction with ZFPM1 and binding to DNA.
Disrupts interaction with ZFPM1. Binds normally to DNA.
Loss of phosphorylation of the chymotryptic peptide.
No effect on DNA binding. Reduces acetylation. Reduces ability to induce erythroid differentiation. Abrogates acetylation; when associated with 312-A--A-316. Abrogates ability to induce erythroid differentiation; when associated with 312-A--A-316. Reduces binding to CREBBP; when associated with 312-A--A-316. Disrupts stable association with chromatin; when associated with 312-A--A-316.
No effect on DNA binding. Reduces acetylation. Reduces binding to CREBBP. Reduces ability to induce erythroid differentiation. Abrogates acetylation; when associated with 245-A-A-246. Abrogates ability to induce erythroid differentiation; when associated with 245-A-A-246. Reduces binding to CREBBP; when associated with 245-A-A-246. Disrupts stable association with chromatin; when associated with 245-A-A-246.
Abolishes interaction with PCNA. No effect on activity.
Loss of activity. No effect on DNA-binding capacity.
AT-rich interactive domain-containing protein 1A
Significant loss of phosphorylation and loss of regulation of ribosomal transcriptional activity.
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C
Phosphotyrosine; by JAK1, JAK2 and PDGFR
L-serine dehydratase/L-threonine deaminase
Almost completely abolishes KLC1-binding.
Marked reduction in KLC1-binding; when associated with A-903. Marked alteration in anterograde axonal transport.
Marked reduction in KLC1-binding; when associated with A-972. Marked alteration in anterograde axonal transport.
Loss of self-renewal in embryonic stem cells in absence of LIF. Does not interact with NCOA3.
Loss of self-renewal in embryonic stem cells in absence of LIF; when associated with A-424. Does not interact with NCOA3; when associated with A-424.
Loss of self-renewal in embryonic stem cells in absence of LIF; when associated with A-425. Does not interact with NCOA3; when associated with A-425.
Methionine aminopeptidase 1D, mitochondrial
eEF1A lysine and N-terminal methyltransferase
Loss of binding to PP2A B-alpha regulatory subunit.
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Megakaryocyte and platelet inhibitory receptor G6b
Long-chain fatty acid transport protein 4
100-fold loss of affinity for PIP3 and accumulation in the cytosol.
Accumulation in proteinaceous aggregates devoid of membranes and no interaction with PI3P.
Hepatocyte growth factor-regulated tyrosine kinase substrate
Loss of protein phosphorylation at Y-329 and Y-334; when associated with A-269.
Loss of protein phosphorylation at Y-329 and Y-334; when associated with A-270.
No change in the phosphorylation level. Loss of protein phosphorylation; when associated with F-329.
No change in the phosphorylation level. Loss of protein phosphorylation; when associated with F-334.
Uncharacterized protein C19orf47 homolog
Confers transcriptional activity at normoxia; when associated with A-530.
Confers transcriptional activity at normoxia; when associated with A-851.
Decreases HRE DNA binding; when associated with D-184.
Decreases HRE DNA binding; when associated with D-186.
Decreases heterodimer formation with ARNT.
Endothelial PAS domain-containing protein 1
Markedly decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with A-171.
Markedly decreases heterodimer formation with ARNT. Impairs heterodimer formation with ARNT; when associated with D-192.
Vacuolar protein sorting-associated protein 26C
ADP-ribosylation factor-like protein 13B
Abolishes palmitoylation and localization to the cilium membrane.
Centriolar coiled-coil protein of 110 kDa
In ketu; homozygotes mice are both male- and female-sterile. In the testis, mutant germ cells carry out an abortive attempt at meiosis: They express hallmark meiotic proteins and initiate recombination, but fail to fully extinguish the spermatogonial mitotic division program, proceed prematurely to an aberrant metaphase-like state, and undergo apoptosis. The mutation probably causes misfolding or protein aggregation. Slightly reduced helicase activity.
Receptor-binding cancer antigen expressed on SiSo cells
DNA-directed RNA polymerase II subunit GRINL1A
Phosphatidylinositol transfer protein alpha isoform
Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.
Results in impaired glucagon binding and glucagon-mediated signaling. Homozygous mice show hyperglucagonemia with alpha-cell hyperplasia and enlargement of the pancreas.
Neuron-specific vesicular protein calcyon
Abrogates interaction with HOOK3. Greatly reduces binding affinity for GDP and GTP. Abolishes GTP-dependent oligomer formation.
Constitutively active. Binds GTP but fails to hydrolyze it. Does not localize to the parasitophorous vacuole membrane following T.gondii infection.
Protein is detected exclusively in the aqueous phase.
Impaired non-canonical autophagy, leading to susceptibility to influenza A virus (IAV) infection in mice.
No effect on the stability of the protein under normal conditions. Enhances cleavage and degradation mediated by activated CASP3 and CASP7. Results in reduced autophagy and defective clearance of intestinal pathogens. Increases secretion of pro-inflammatory cytokine IL1B and type I IFN.
Acyl-coenzyme A synthetase ACSM3, mitochondrial
Almost complete loss of transcriptional activation; when associated with A-437.
Almost complete loss of transcriptional activation; when associated with A-438.
Almost no effect on transcriptional activation in response to viral infection.
Increased transcriptional activation; when associated with D-437.
Increased transcriptional activation; when associated with D-438.
No effect on transcriptional activation in response to viral infection.
Strongly decreased transcriptional activation in response to viral infection.
Strongly decreased transcriptional activation in response to viral infection; when associated with A-425.
Strongly decreased transcriptional activation in response to viral infection; when associated with A-426.
Strongly decreased transcriptional activation in response to viral infection; when associated with D-425.
Strongly decreased transcriptional activation in response to viral infection; when associated with D-426.
Strongly increased transcriptional activation in response to viral infection.
Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1
All-trans-retinol dehydrogenase [NAD(+)] ADH7
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
Glycosyltransferase 8 domain-containing protein 1
Decreased protein abundance. Not associated with changes in subcellular location.
Decreased protein abundance. Not delivered to the plasma membrane.
Decreased protein abundance. Not delivered to the plasma membrane. Heterozygotes mice with this mutation display abnormal social behavior.
N-linked (GlcNAc...) (complex) asparagine
No effect on protein abundance. Not associated with changes in subcellular location.
Not associated with changes in subcellular location.
Abolishes both the nuclear localization and the enzyme activity.
Abolishes the nuclear localization but does not affect the enzyme activity; when associated with A-198.
Abolishes the nuclear localization but does not affect the enzyme activity; when associated with A-201.
Does not affect neither the nuclear localization nor the enzyme activity.
Does not affect the nuclear localization but affects the enzyme activity.
Does not affect the nuclear localization.
Does not strongly affect the nuclear localization but strongly affects the enzyme activity.
Helical; Anchor for type IV membrane protein
Sodium-coupled neutral amino acid symporter 2
Highly susceptible to mouse cytomegalovirus infection. Shows low level of cytokine induction and natural killer activation on viral infection.
Significantly decreased NF-kappa-B activation.
Heart- and neural crest derivatives-expressed protein 2
Baculoviral IAP repeat-containing protein 1f
Mediator of RNA polymerase II transcription subunit 20
ATP synthase subunit gamma, mitochondrial
Methylglutaconyl-CoA hydratase, mitochondrial
Decrease in autoubiquitination; when associated with S-13.
Decrease in autoubiquitination; when associated with S-16.
Zinc-regulated GTPase metalloprotein activator 1
PH domain leucine-rich repeat-containing protein phosphatase 2
Serine/threonine-protein kinase MRCK beta
Epidermal growth factor receptor kinase substrate 8-like protein 3
TRAF3-interacting JNK-activating modulator
Asymmetric dimethylarginine; by CARM1; alternate
Asymmetric dimethylarginine; by PRMT6; alternate
Phosphoserine; alternate; by AURKB, AURKC and RPS6KA5
Phosphoserine; alternate; by AURKB, AURKC, RPS6KA3, RPS6KA4 and RPS6KA5
Symmetric dimethylarginine; by PRMT5; alternate
No formation of homomultimers. No formation of homodimers; when associated with S-369.
No formation of homomultimers. No formation of homodimers; when associated with S-393.
von Willebrand factor A domain-containing protein 1
Reduced interaction with GORASP2; when associated with A-393.
Reduced interaction with GORASP2; when associated with A-396.
Thrombospondin-type laminin G domain and EAR repeat-containing protein
Histone deacetylase complex subunit SAP25
Solute carrier family 2, facilitated glucose transporter member 9
Mas-related G-protein coupled receptor member B4
Phosphatidylinositol 4-kinase type 2-alpha
Cilia- and flagella-associated protein 119
Chromatin accessibility complex protein 1
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
Ubiquitin carboxyl-terminal hydrolase 48
Reduced binding to U6 snRNA without affecting binding to 7SK RNA. Reduced 2'-O-methylation of U6 snRNAs.
Impairs DNA-binding and phosphorylation by MAPK1.
Increase in the kinase activation level.
S-adenosylmethionine synthase isoform type-2
Nuclear transcription factor Y subunit alpha
Disrupts interaction with CIB2 and CIB3.
No impact on interaction with CIB2 and CIB3.
Ubiquitin-associated domain-containing protein 2
Higher level of processed SREBF1 and SREBF2 when expressed in liver.
Loss of ubiquitination; when associated with R-454.
Loss of ubiquitination; when associated with R-466.
Sterol regulatory element-binding protein cleavage-activating protein
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)
Interferon-stimulated gene 20 kDa protein
Abolishes maturation and cleavage by CORIN.
Does not affect maturation and cleavage by CORIN.
Peptidyl-tRNA hydrolase 2, mitochondrial
CAP-Gly domain-containing linker protein 4
Abolishes localization at the cell membrane.
Decreased permeability to chloride ions.
Decreased threshold for activation by calcium.
Increased permeability to chloride ions.
Increased threshold for activation by calcium.
Increased threshold for activation by calcium. Increased permeability to chloride ions.
Low constitutive channel activity. Decreased threshold for activation by calcium.
Moderately increased threshold for activation by calcium.
No effect of cysteine-modifying agent MTSET on ion permeability in contrast to wild-type where MTSET blocks current.
No effect on threshold for activation by calcium.
Reduced anion permeability and increased cation permeability.
Strongly increased threshold for activation by calcium.
WD repeat and FYVE domain-containing protein 3
Fails to bind to NIH-3T3 fibroblast cells; when associated with 273-A--A-276.
Fails to bind to NIH-3T3 fibroblast cells; when associated with A-291.
Fails to bind to heparan sulfate or to NIH-3T3 fibroblast cells; when associated with A-238 and A-244.
Fails to bind to heparan sulfate or to NIH-3T3 fibroblast cells; when associated with A-238 and A-295.
Fails to bind to heparan sulfate or to NIH-3T3 fibroblast cells; when associated with A-244 and A-295.
No effect on binding to NIH-3T3 fibroblast cells.
No effect on binding to NIH-3T3 fibroblast cells; when associated with A-109.
No effect on binding to NIH-3T3 fibroblast cells; when associated with A-216.
Mitochondrial import inner membrane translocase subunit TIM22
Caspase recruitment domain-containing protein 19
Serine hydroxymethyltransferase, cytosolic
Interferon regulatory factor 2-binding protein 1
Low-density lipoprotein receptor-related protein 5
Leucine-rich repeat-containing protein 75A
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Transmembrane domain-containing protein TMIGD3
Myb/SANT-like DNA-binding domain-containing protein 2
ATP-binding site mutant; unable to activate MAPKAPK5.
Kinase defective mutant, abolishes activity.
Unable to activate MAPKAPK5 promote MAPKAPK5 localization to the cytoplasm.
Specifically androgen-regulated gene protein
Ral guanine nucleotide dissociation stimulator-like 2
Cytoplasmic phosphatidylinositol transfer protein 1
WD repeat-containing and planar cell polarity effector protein fritz homolog
Abolishes the binding to CDK5RAP1, CDK5RAP2, CDKRAP3, but not to CDK5.
Cyclin-dependent kinase 5 activator 1, p25
Cyclin-dependent kinase 5 activator 1, p35
Endoplasmic reticulum junction formation protein lunapark
Ras GTPase-activating-like protein IQGAP1
Tumor necrosis factor receptor superfamily member 23
Immediate early response 3-interacting protein 1
Baculoviral IAP repeat-containing protein 3
Protein phosphatase 1 regulatory subunit 21
Ubiquitin carboxyl-terminal hydrolase 17-like protein A
Does not affect mitochondrial localization and ability to mediate calcium efflux from mitochondrion.
Does not affect mitochondrial localization but abolishes ability to mediate calcium efflux from mitochondrion.
Mitochondrial proton/calcium exchanger protein
Decreases inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-295 and F-319.
Decreases inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-295 and F-349.
Decreases inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-319 and F-349.
Strong inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-295 and F-335.
Strong inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-295 and F-349.
Strong inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-319 and F-335.
Strong inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-319 and F-349.
Strong inhibitory signaling in germinal center formation (absence of SH2D1A/SAP); when associated with F-335 and F-349.
Pleckstrin homology domain-containing family H member 2
Gamma-aminobutyric acid type B receptor subunit 2
Protein phosphatase 1 regulatory subunit 42
Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial
Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-44.
Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-46.
Developmental pluripotency-associated protein 3
Coiled-coil and C2 domain-containing protein 1A
Increases activity towards short-chain fatty acids.
Lowers activity towards short-chain fatty acids.
Lowers activity towards short-chain fatty acids. Strong increase in activity towards medium chain fatty acids.
Almost complete loss of exonuclease activity in TDT-L; when associated with A-170 and A-473. Decreased transferase activity in TDT-S; when associated with A-170 and A-473.
Almost complete loss of exonuclease activity in TDT-L; when associated with A-29 and A-170. Decreased transferase activity in TDT-S; when associated with A-29 and A-170.
Almost complete loss of exonuclease activity in TDT-L; when associated with A-29 and A-473. Decreased transferase activity in TDT-S; when associated with A-29 and A-473.
Solute carrier organic anion transporter family member 1A6
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
Cysteine sulfenic acid (-SOH) intermediate
Zinc finger and BTB domain-containing protein 9
Peptidyl-prolyl cis-trans isomerase FKBP9
Does not inhibit NFAT-induced transcription activation.
Abolishes interaction with IRS1 but not with IRS2.
Echinoderm microtubule-associated protein-like 1
Galactose-1-phosphate uridylyltransferase
Loss of nuclear localization. Reduces DNA binding.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
Fibrinogen C-terminal domain-containing protein
Regenerating islet-derived protein 3-beta 15 kDa form
Regenerating islet-derived protein 3-beta 16.5 kDa form
Regulation of nuclear pre-mRNA domain-containing protein 1A
Leukocyte receptor cluster member 1 homolog
39S ribosomal protein L34, mitochondrial
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, mitochondrial
Protein phosphatase 1 regulatory subunit 35
Chorion-specific transcription factor GCMb
Impairs RHOA activation; when associated with 566-A-A-567.
Impairs RHOA activation; when associated with A-562.
No effect on RHOA activation; when associated with 547-A-A-548.
No effect on RHOA activation; when associated with 554-A-A-555.
Rho guanine nucleotide exchange factor 15
Complete loss of STAT3 activation; in association with F-443.
Complete loss of STAT3 activation; in association with F-493.
Fibronectin type-III domain-containing protein
Serine/threonine-protein kinase/endoribonuclease IRE2
non-specific serine/threonine protein kinase
Actin, gamma-enteric smooth muscle, intermediate form
DXXLL motif involved in the interaction with GGA1
Potential nuclear localization signal for the C-terminal fragment generated by PSEN1
Lysosomal acid lipase/cholesteryl ester hydrolase
Signal recognition particle receptor subunit alpha
Homozygous mice display spontaneous generalized seizures and premature death between P16 and P26. Heterozygous mice show infrequent spontaneous seizures, increased susceptibility to chemically and hyperthermia-induced generalized seizures and sleep abnormalities. Heterozygous mice also show deficits in social behavior, spatial memory, cued fear conditioning, pre-pulse inhibition and risk assessment.
Interchain; with the conotoxin GVIIJ (when the channel is not linked to SCN2B or SCN4B; the bond to SCN2B or SCN4B protects the channel from the inhibition by toxin)
Sodium channel protein type 1 subunit alpha
Uncharacterized protein C19orf44 homolog
NACHT, LRR and PYD domains-containing protein 12
POU domain, class 3, transcription factor 2
Cyclic nucleotide-gated olfactory channel
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
Conserved oligomeric Golgi complex subunit 8
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2
Mitochondrial-processing peptidase subunit beta
Loss of kinase activity and ability to activate JNK family. Inhibits the ability of CLNK to up-regulate CD3-triggered activation of the IL2 promoter.
Mitogen-activated protein kinase kinase kinase kinase 1
Putative methyltransferase C9orf114 homolog
N-acetylvaline; in Peptidyl-prolyl cis-trans isomerase A, N-terminally processed
Peptidyl-prolyl cis-trans isomerase A, N-terminally processed
Phosphoribosyl pyrophosphate synthase-associated protein 2
Mediator of RNA polymerase II transcription subunit 27
Dihydrolipoyl dehydrogenase, mitochondrial
ATP synthase subunit ATP5MPL, mitochondrial
Gain-of-function mutation; causes an alopecia phenotype.
Impaired ability to induce pyroptosis; when associated with A-132.
In AE mutant; gain-of-function mutation; causes an alopecia phenotype; constitutively active in triggering cell death. Mice also display hypoplastic mammary glands, impairing lactation function.
In AP1; promotes ability to release of interleukin-1 (IL1B and IL18) precursors.
In AP2; promotes ability to release of interleukin-1 (IL1B and IL18) precursors.
In Bsk mutant; gain-of-function mutation; causes an alopecia phenotype. Attenuates intramolecular interaction between the N- and C-terminal domains, hence may relieve autoinhibition; constitutively active in triggering pyroptosis.
In Dfl mutant; gain-of-function mutation; causes an alopecia phenotype.
In Fgn mutant; gain-of-function mutation; causes an alopecia phenotype. Attenuates intramolecular interaction between the N- and C-terminal domains, hence may relieve autoinhibition; constitutively active in triggering pyroptosis.
In Rco2 mutant; gain-of-function mutation; causes an alopecia phenotype. Attenuates intramolecular interaction between the N- and C-terminal domains, hence may relieve autoinhibition; constitutively active in triggering pyroptosis.
In Re-den mutant; gain-of-function mutation; causes an alopecia phenotype. Attenuates intramolecular interaction between the N- and C-terminal domains, hence may relieve autoinhibition; constitutively active in triggering pyroptosis.
In Rim3 mutant; gain-of-function mutation; causes an alopecia phenotype. Attenuates intramolecular interaction between the N- and C-terminal domains, hence may relieve autoinhibition; constitutively active in triggering pyroptosis.
Markedly decreased induction of pyroptosis and defects in liposome-binding. No spontaneous pyroptosis-inducing activity; when associated with K-14.
No spontaneous pyroptosis-inducing activity; when associated with D-184.
Spontaneous pyroptosis-inducing activity.
Weak or no effect on ability to induce pyroptosis. Impaired ability to induce pyroptosis; when associated with A-145.
Proheparin-binding EGF-like growth factor
Transmembrane emp24 domain-containing protein 4
Abolished phosphorylation by GSK3 and decreased acetyltransferase activity. Impaired ability to activate the cGAS-STING antiviral response in knockin mice. Lean phenotype caused by impaired ability to promote the synthesis of diacylglycerol in knockin mice. Decreased ability to promote homologous recombination (HR)repair in response to DNA double-strand breaks (DSBs).
Impaired phosphorylation and decreased acetyltransferase activity, leading to decreased ability to promote homologous recombination (HR)repair in response to DNA double-strand breaks (DSBs).
Mimics phosphorylation; constitutively active mutant that shows constitutive protein acetyltransferase activity. Increased ability to promote homologous recombination (HR)repair in response to DNA double-strand breaks (DSBs).
Mimics phosphorylation; constitutively active mutant that shows constitutive protein acetyltransferase and acyltransferase activities in knockin mice. Increased ability to promote homologous recombination (HR)repair in response to DNA double-strand breaks (DSBs).
Coiled-coil domain-containing protein 113
Eukaryotic translation initiation factor 4E type 3
Cytochrome c oxidase subunit 4 isoform 2, mitochondrial
Uncharacterized protein C1orf100 homolog
Abolishes exonuclease activity without affecting the telomere localization, leading to impaired 3'-overhang at the leading end telomeres.
Abolishes exonuclease activity, leading to activate the ATM signaling pathway; when associated with 31-A--N-35.
Abolishes exonuclease activity, leading to activate the ATM signaling pathway; when associated with A-230.
Abolishes interaction with TERF2 and localization to telomeres, leading to activate the ATM signaling pathway.
Asymmetric dimethylarginine; by PRMT1; alternate
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1); alternate
Omega-N-methylarginine; by PRMT1; alternate
Symmetric dimethylarginine; by PRMT5 and PRMT7; alternate
2'-5'-oligoadenylate synthase-like protein 1
No loss of dsRNA binding capacity. Binding capacity was reduced 40-60%; when associated with A-203. Binding capacity was reduced to 5-10%; when associated with A-152 and A-203. Loss of binding capacity; when associated with A-152; A-203 and A-257.
No loss of dsRNA binding capacity. Binding capacity was reduced 40-60%; when associated with A-91. Binding capacity was reduced to 5-10%; when associated with A-91 and A-152. Loss of binding capacity; when associated with A-91; A-152 and A-257.
No loss of dsRNA binding capacity. Loss of 40-60% dsRNA binding; when associated with A-203. Binding capacity was reduced to 5-10%; when associated with A-91 and A-203. Loss of binding capacity; when associated with A-91; A-203 and A-257.
No loss of dsRNA binding capacity. Loss of 5-10 % dsRNA binding capacity; when associated with A-152 and A-203. Loss of binding capacity; when associated with A-91; A-152 and A-203.
Calcium permeable stress-gated cation channel 1
Paired immunoglobulin-like type 2 receptor alpha
Large neutral amino acids transporter small subunit 1
Low density lipoprotein receptor adapter protein 1
Does not affect the interaction with VCP.
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Extracellular matrix organizing protein FRAS1
Abolished oligomerization without affecting interaction with KEAP1; when associated with A-69.
Abolished oligomerization without affecting interaction with KEAP1; when associated with A-7.
Does not affect interaction with KEAP1. Decreased phosphorylation by MTOR.
Greatly decreases interaction with MAP1LC3B.
Phosphomimetic mutant; promotes interaction with KEAP1 and nuclear accumulation of NFE2L2/NRF2.
Strongly decreased interaction with KEAP1.
Transmembrane emp24 domain-containing protein 3
Angiogenic factor with G patch and FHA domains 1
12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
NEDD8-activating enzyme E1 regulatory subunit
Abolishes deglutamylase activity; when associated with Q-806.
Abolishes deglutamylase activity; when associated with S-803.
Fibronectin type III domain-containing protein 5
KN motif and ankyrin repeat domain-containing protein 2
Isochorismatase domain-containing protein 2A
Abolishes interaction with the SH3 domain of STAM2.
Abolishes the interaction with YWHAE and leads to accumulation in the nucleus.
Does not affect interaction with the SH3 domain of STAM2.
Impairs deubiquitination of EPS15, RNF128 and RNF41.
Reduces interaction with the SH3 domain of STAM2.
Reduces stabilization activity on RNF41.
Actin filament-associated protein 1-like 1
Less efficiently phosphorylated in vitro.
ADP-ribosylation factor-like protein 2-binding protein
Lymphokine-activated killer T-cell-originated protein kinase
Uncharacterized protein C6orf163 homolog
p53-induced death domain-containing protein 1
Complete loss RAN-GEF activity. Partial loss of RAN-binding.
Lysosomal targeting motif. Required for AP1G1, AP2A2 and AP3D1 interaction
Cytosolic 10-formyltetrahydrofolate dehydrogenase
AT-rich interactive domain-containing protein 4B
Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-23.
Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-24.
DRY motif; important for ligand-induced conformation changes and signaling
NPxxY motif; important for ligand-induced conformation changes and signaling
Rho-related BTB domain-containing protein 3
Significant reduction of apoptotic activity.
Ras/Rap GTPase-activating protein SynGAP
Abolishes phosphorylation by PKA and impairs nuclear export following ACTH treatment.
Does not affect phosphorylation by PKA and nuclear export following ACTH treatment.
Localizes mainly in cytoplasm and not in nucleus.
Localizes mainly in cytoplasm and not in nucleus; when associated with D-483 and A-607.
Localizes mainly in cytoplasm and not in nucleus; when associated with D-483 and A-610.
Dehydrogenase/reductase SDR family member 11
Loss of nucleotidyltransferase activity and oocytes are unable to support early embryonic development; when associated with A-1026.
Loss of nucleotidyltransferase activity and oocytes are unable to support early embryonic development; when associated with A-1028.
No effect on basal uridylation activity, but loss of LIN28A-enhanced uridylation; when associated with A-326.
No effect on basal uridylation activity, but loss of LIN28A-enhanced uridylation; when associated with A-329.
39S ribosomal protein L14, mitochondrial
Fibronectin type III domain-containing protein 3B
Coiled-coil domain-containing protein 85B
In 2DA mutant; abolished cleavage by caspase, preventing phospholipid scramblase activity.
Maestro heat-like repeat-containing protein family member 2B
U11/U12 small nuclear ribonucleoprotein 48 kDa protein
C-1-tetrahydrofolate synthase, cytoplasmic
C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed
Ras GTPase-activating protein-binding protein 1
Pyrin and HIN domain-containing protein 1-like
Cilia- and flagella-associated protein 298
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
Cell cycle exit and neuronal differentiation protein 1
Polyribonucleotide 5'-hydroxyl-kinase Clp1
Progressive loss of motor neurons, axonal motor neuropathy, and muscle paralysis leading to death within hours of birth. Mice exhibit microcephaly due to reduced numbers of cortical neurons. Embryos have normal numbers and proliferation of neuronal progenitors, but neuronal progenitor cells undergo enhanced cell death, resulting in reduced numbers of cortical neurons.
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Coiled-coil domain-containing protein 106
Abolishes sumoylation. No effect on activation of OCT4 but activation not suppressed by additional SUMO1. Enhanced interaction with KAT2B; when associated with A-210 or E-210.
Abolishes sumoylation. No repression of OCT4 gene expression with or without retinoic acid and enhanced interaction with KAT2B and HDAC3. Abolishes interaction with HDAC3 and PML association; when associated with R-238.
Abolishes sumoylation. Strongly associated with PML nuclear bodies. No effect on activation of OCT4 but activation not suppressed by additional SUMO1. Increased binding to KAT2B and reduced binding to NRIP1. Abolishes PML association; when associated with A-210.
Greatly reduced PKC-stimulated protein stability and activation of RARB reporter.
Little effect on PKC-stimulated protein stability nor on activation of RARB reporter.
No effect on sumoylation and little effect on binding KAT2B. Some reduced DNA-binding. Greatly reduced DNA-binding, binding to KAT2B and activation of the RARB promoter; when associated with A-170.
No effect on sumoylation nor on DNA-binding and little effect on binding KAT2B. Greatly reduced DNA-binding, binding to KAT2B and activation of the RARB promoter; when associated with A-185.
Nuclear receptor subfamily 2 group C member 1
Sumoylated. Repressed OCT4 gene expression. Enhanced interaction with KAT2B; when associated with R-238.
Embryos have congenital heart defects with transposition of the great artery, 61% exhibiting situs inversus or heterotaxy and 39% exhibiting situs solitus. This mutation corresponds to the pathological variant 'T-226' identified in humans.
Koli mutant, animals exhibit visceral heterotaxy, with heart defects commonly associated with heterotaxy.
Miri mutant, animals exhibit visceral heterotaxy, with heart defects commonly associated with heterotaxy.
Pro-neuregulin-3, membrane-bound isoform
Heterogeneous nuclear ribonucleoprotein F
Heterogeneous nuclear ribonucleoprotein F, N-terminally processed
N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processed
Required for SUMO paralog-specific binding
Ubiquitin carboxyl-terminal hydrolase 25
Flagellum-associated coiled-coil domain-containing protein 1
Abolishes DNA-binding and transcriptional activity.
Abolishes monomethylation by SETD6 and interaction with EHMT1.
Abolishes sulfhydration and impairs interaction with RPS3.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO3)
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO3); alternate
N6-acetyllysine; by PCAF and EP300; alternate
Pleckstrin homology domain-containing family S member 1
In isoform 2, isoform 3, isoform 4, isoform 5, isoform 6, isoform 10, isoform 12 and isoform 16.
In isoform 2, isoform 3, isoform 4, isoform 7, isoform 8, isoform 10, isoform 11, isoform 12, isoform 13, isoform 14 and isoform 16.
In isoform 2, isoform 3, isoform 5, isoform 6, isoform 9, isoform 13, isoform 14 and isoform 16.
In isoform 2, isoform 3, isoform 7, isoform 8, isoform 9, isoform 12, isoform 14 and isoform 15.
In isoform 2, isoform 4, isoform 6, isoform 8, isoform 9, isoform 12, isoform 13, isoform 14, isoform 15 and isoform 16.
Gamma-aminobutyric acid receptor subunit alpha-5
Regulator of microtubule dynamics protein 3
X-linked interleukin-1 receptor accessory protein-like 2
Cell division cycle-associated protein 4
Cysteine-rich secretory protein LCCL domain-containing 2
Microtubule-associated serine/threonine-protein kinase 3
Lipocln_cytosolic_FA-bd_dom domain-containing protein
In strain: C57BL/6J; could be associated with tumor susceptibility; does not affect binding with tubulin; more unstable.
Oxysterol-binding protein-related protein 5
Schiff-base intermediate with substrate; via topaquinone
Dihydroxyacetone phosphate acyltransferase
ADP-ribose pyrophosphatase, mitochondrial
Leucine-rich repeat and transmembrane domain-containing protein 2
Guanine nucleotide-binding protein G(t) subunit alpha-3
5'-nucleotidase domain-containing protein 3
Serine/threonine-protein kinase greatwall
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
In a mouse model for harlequin ichthyosis (HI), homozygous mice are embryonic lethal but occasionally pups are found in the first few hours after birth but die and are severely dehydrated and fail to suckle normally. Homozygous pups show hallmarks of HI desease including hyperkeratosis, abnormal extracellular lipid lamellae and defects in cornified envelope processing. At 14.5 dpc and 15.5 dpc homozygous embryos appear normal; however from 16.5 dpc onwards they are characterized by an absence of normal skin folds around the trunk and limbs. As development progressed, embryos develop a taut, thick epidermis and multiple contractures affecting the limbs. Late stage embryos are smaller.
In a mouse model for harlequin ichthyosis (HI), smooth skin (smsk) mutant mice show a pronounced perinatal lethal skin phenotype in 25% of the offspring and newborn mutant pups die within a few hours after birth, and appear severely dehydrated with dry cracking skin. Smsk homozygous mutants embryos show a normal appearance at 14.5 dpc, but at 16.5 dpc develop a partial absence of normal skin folds around the trunk and limbs, and by 18.5 dpc develop a taut, thick skin and limb contractures.
Does not affect formation of liquid-liquid phase separation (LLPS).
Impaired formation of liquid-liquid phase separation (LLPS), leading to reduced formation of the NLRP6 inflammasome and GSDMD-dependent release of IL18.
NACHT, LRR and PYD domains-containing protein 6
BTB/POZ domain-containing protein KCTD16
39S ribosomal protein L37, mitochondrial
Platelet-derived growth factor receptor alpha
Loss of catalytic activity. No effect on the redistribution of AP-1 from the membrane to the cytoplasm.
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Decreased enzyme activity to retain 63% of wild type activity.
contacts bases in the GGAA sequence in the major groove
contacts the phosphate backbone of the GGAA sequence in the minor groove upstream
forms a salt bridge with the phosphate backbone of the opposite strand downstream of the GGAA core sequence
Ankyrin repeat domain-containing protein 1
Golgi-associated PDZ and coiled-coil motif-containing protein
Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase
Selection and upkeep of intraepithelial T-cells protein 1
Inactive 2'-5' oligoadenylate synthetase 1C
Abolished localization to the nucleus, leading to impaired ability to regulate alternative splicing of Ttn (Titin) mRNAs.
Decreased localization to the nucleus, leading to impaired ability to regulate alternative splicing of Ttn (Titin) mRNAs.
Impaired ability to regulate alternative splicing of Ttn (Titin) mRNAs.
Knockin mice show severe cardiac phenotypes, characterized by dilated cardiomyopathy and atrial fibrillation. Decreased localization to the nucleus, leading to impaired ability to regulate alternative splicing of Ttn (Titin) mRNAs.
Knockin mice show severe cardiac phenotypes, characterized by dilated cardiomyopathy, leading to premature death. Decreased localization to the nucleus associated with an increased localization to cytoplasmic ribonucleoprotein granules.
Mediator of RNA polymerase II transcription subunit 12
Protein phosphatase 1 regulatory subunit 3F
Ubiquitin-conjugating enzyme E2 variant 1
Rho guanine nucleotide exchange factor 2
LPXY motif; mediates binding to WW domain-containing proteins
PPXY motif; mediates binding to WW domain-containing proteins
Transmembrane gamma-carboxyglutamic acid protein 4
Zinc finger and BTB domain-containing protein 24
Centriolar satellite-associated tubulin polyglutamylase complex regulator 1
5-glutamyl glycerylphosphorylethanolamine
N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT3
N6,N6-dimethyllysine; alternate; by EEF1AKMT3
N6-methyllysine; alternate; by EEF1AKMT3
Cytochrome b-c1 complex subunit Rieske, mitochondrial
Decreased localization to the nucleus. No effect on homodimerization. Decreased transcription cis-regulatory region binding. Loss of DNA-binding transcription factor activity. Acts as a dominant negative.
Decreased localization to the nucleus. No effect on homodimerization. No effect on transcription cis-regulatory region binding. No effect on DNA-binding transcription factor activity.
Increased localization to the nucleus. Decreased transcription cis-regulatory region binding. No effect on DNA-binding transcription factor activity.
Loss of localization to the nucleus. Loss of transcription cis-regulatory region binding. Loss of DNA-binding transcription factor activity. Acts as a dominant negative.
Iron-sulfur cluster assembly 2 homolog, mitochondrial
Increased phosphorylation. Increases muscle specific force.
Loss of phosphorylation by AMPK. Decreases muscle specific force.
Calcium-independent phospholipase A2-gamma
Cilia- and flagella-associated protein 70
N-linked (GlcNAc...) asparagine; partial
No loss of repressor activity, somite segmentation disrupted at later stages.
tRNA wybutosine-synthesizing protein 2 homolog
ATP synthase F(0) complex subunit C2, mitochondrial
Major facilitator superfamily domain-containing protein 4A
Abolishes surface expression and channel activity.
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Reduces surface expression and abolishes channel activity.
Reduces surface expression and decreases current amplitude.
Reduces surface expression and disrupts channel closure.
Shifts channel activation to more negative voltage, slows channel opening and speeds up channel closure. Reduces sensitivity to activation by cAMP.
Shifts voltage dependence of activation to more negative values.
Shifts voltage dependence of activation to more positive values and abolishes pH-sensitivity.
Signal peptidase complex catalytic subunit SEC11A
Katanin p60 ATPase-containing subunit A-like 2
DNA repair and recombination protein RAD54B
All-trans retinoic acid-induced differentiation factor
Abolishes CIDE-N/CIDE-N interaction between the 2 homodimer subunits and inhibits lipid droplet enlargement. No effect on homodimerization.
Abolishes CIDE-N/CIDE-N interaction between the 2 homodimer subunits.
Abolishes lipid droplet enlargement activity, but not localization to lipid droplets, nor enrichement at contact sites; when associated with A-182 and A-186.
Abolishes lipid droplet enlargement activity, but not localization to lipid droplets, nor enrichement at contact sites; when associated with A-182 and A-190.
Abolishes lipid droplet enlargement activity, but not localization to lipid droplets, nor enrichement at contact sites; when associated with A-186 and A-190.
Inhibits interaction with PLIN1; when associated with A-75.
Inhibits interaction with PLIN1; when associated with A-77.
No effect on protein stability. Drastically increased protein stability and decreased ubiquitination; when associated with A-224 and A-226.
No effect on protein stability; when associated with A-226. Drastically increased protein stability and decreased ubiquitination; when associated with A-224 and A-236.
No effect on protein stability; when associated with A-226. Drastically increased protein stability and decreased ubiquitination; when associated with A-226 and A-236.
Reduces CIDE-N/CIDE-N interaction between the 2 homodimer subunits and inhibits lipid droplet enlargement.
Slightly inhibits interaction with PLIN1.
Slightly inhibits interaction with PLIN1; when associated with A-112 or A-117.
Slightly inhibits interaction with PLIN1; when associated with A-115.
Nuclear receptor subfamily 5 group A member 2
CCR4-NOT transcription complex subunit 6-like
Testis-specific serine/threonine-protein kinase 3
Leucine-rich repeat-containing protein 42
Ankyrin repeat domain-containing protein 10
Delta and Notch-like epidermal growth factor-related receptor
Fails to be restricted to the somatodendritic compartment.
Vacuolar protein sorting-associated protein 51 homolog
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Decreased selectivity for monovalent cations and increased permeability for Ca(2+).
Decreases channel sensitivity to inhibition by ATP.
Near loss of channel sensitivity to inhibition by ATP.
Strongly decreased selectivity for monovalent cations and increased permeability for Ca(2+).
Strongly decreases channel sensitivity to inhibition by ATP.
Transient receptor potential cation channel subfamily M member 4
Causes malformations in the distal reproductive tract development in both female and male.
LHFPL tetraspan subfamily member 2 protein
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)
CKLF-like MARVEL transmembrane domain-containing protein 2B
Hydroxymethylglutaryl-CoA synthase, cytoplasmic
Pre-B-cell leukemia transcription factor 1
Significantly reduced binding to NFIL3 promoter.
Slightly reduced binding to NFIL3 promoter.
Equivalent to human variant V66E involved in CTS2 disease. Reduces secretion in tendon cells and nhibits oligomerization. Mutant mice develop inflammation and fibrosis in Achilles tendons and carpal tunnel. They show compromised regenerative capability of tendons.
Acyl-CoA synthetase short-chain family member 3, mitochondrial
Abolished cleavage and activation; when associated with A-198.
Abolished cleavage and activation; when associated with A-23.
Gamma-aminobutyric acid receptor subunit pi
N-linked (GlcNAc...) (high mannose) asparagine
Premature lethality from 3 months of age, with no survival beyond 6 months. Progressive defects in motor coordination and balance from age 3 months, accompanied by a twitching phenotype. Marked degeneration of astrocytes in the cerebrum. Significant loss of peptidase activity, leading to accumulation of the TPP1 substrate ATP5MC1 in lysosomes.
39S ribosomal protein L39, mitochondrial
Small conductance calcium-activated potassium channel protein 3
Oncoprotein-induced transcript 3 protein
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
Guanine nucleotide exchange factor for Rab-3A
Activating signal cointegrator 1 complex subunit 3
Abolishes localization to mitochondria in fat cells.
Schiff-base intermediate with DNA; for 5'- deoxyribose-5-phosphate lyase activity
Schiff-base intermediate with DNA; for 5'-deoxyribose-5-phosphate lyase activity
X-ray repair cross-complementing protein 6
Maestro heat-like repeat-containing protein family member 7
BRD4-interacting chromatin-remodeling complex-associated protein-like
Solute carrier family 2, facilitated glucose transporter member 4
Abrogates RNA-binding by DRBM 3 and interaction with XPO5 and nuclear export; when associated with A-235.
Abrogates RNA-binding by DRBM 3 and interaction with XPO5 and nuclear export; when associated with A-237.
Double-stranded RNA-binding protein Staufen homolog 2
Prevents nuclear localization of mutants lacking DRBM 3 function; when associated with 257-AA-258.
Prevents nuclear localization of mutants lacking DRBM 3 function; when associated with 289-AAA-291.
Granulocyte colony-stimulating factor receptor
CMT1A duplicated region transcript 4 protein homolog
Polyamine-modulated factor 1-binding protein 1
Cilia- and flagella-associated protein 52
Abolished phosphorylation by RPS6KA3/RSK2.
Cyclic AMP-dependent transcription factor ATF-4
Does not affect phosphorylation by RPS6KA3/RSK2.
Promotes stabilization due to impaired ubiquitination.
Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-230 and A-234. Does not affect phosphorylation by RPS6KA3/RSK2.
Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-230 and A-247.
Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-234 and A-247.
Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-230; A-234 and A-247.
Promotes stabilization due to impaired ubiquitination; when associated with A-223; A-230; A-234 and A-247.
Spermatid-specific linker histone H1-like protein
Ciliary neurotrophic factor receptor subunit alpha
Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2
Mitogen-activated protein kinase kinase kinase 19
Uncharacterized protein KIAA0930 homolog
Organic solute transporter subunit alpha
Homozygous mice show severe morphological and skeletal abnormalities including short stature along with limb, rib, pelvis, and skull deformities with minimal trabecular bone and reduced cortical bone thickness in long bones.
Gamma-aminobutyric acid receptor-associated protein-like 1
Phosphatidylethanolamine amidated glycine; alternate
Phosphatidylserine amidated glycine; alternate
39S ribosomal protein L54, mitochondrial
ADP-ribosylation factor GTPase-activating protein 2
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)
Interferon-induced helicase C domain-containing protein 1
S-(15-deoxy-Delta12,14-prostaglandin J2-9-yl)cysteine
Ribosomal RNA-processing protein 7 homolog A
Introduces an N-glycosylation site and disrupts interaction with NGF and BDNF.
Retains 2% of the initial activity; deacylation of the acyl-enzyme intermediate becomes rate-limiting.
39S ribosomal protein L28, mitochondrial
Mothers against decapentaplegic homolog 3
Phosphothreonine; by CDK2, CDK4 and MAPK
Interferon-induced protein with tetratricopeptide repeats 3
DNA replication complex GINS protein PSF1
Tumor necrosis factor receptor superfamily member 21
Putative aspartate aminotransferase, cytoplasmic 2
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
snRNA-activating protein complex subunit 1
In slaty; decreased DOPAchrome tautomerase activity.
Mutant mice show an hypopigmentation of the coat and have the retinal pigmented epithelium significantly less pigmented than wild-type retinas.
Histone-lysine N-methyltransferase SETD7
NADH dehydrogenase [ubiquinone] 1 subunit C2
Phospholipid phosphatase-related protein type 2
Leucine-rich repeat-containing protein 51
Abolished binding to and nuclear export of hypusinated EIF5A.
U6 snRNA-associated Sm-like protein LSm2
In strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, CAST/Ei, DBA/2J, FVB/N, ST/bJ and SWR/J.
In strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J.
In strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J and DBA/2J; may influence the ability to form dimers or bind sweeteners.
In strain: 129/J, 129/SvEv, AKR/J, BALB/c, C3H/HeJ, DBA/2J, DBA/2J, FVB/N, ST/bJ and SWR/J.
In strain: 129/J, 129/SvEv, AKR/J, BALB/c, CAST/Ei, C3H/HeJ, DBA/2J, FVB/N, ST/bJ and SWR/J.
In strain: 129/J, AKR/J, CAST/Ei, DBA/2J and SWR/J.
N-linked (GlcNAc...) asparagine; when associated with variant T-60
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Doublesex- and mab-3-related transcription factor C2
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3
Epidermal growth factor receptor substrate 15
Loss of interaction with UBQLN1; when associated with A-863 and A-864.
Loss of interaction with UBQLN1; when associated with A-863 and A-865.
Loss of interaction with UBQLN1; when associated with A-864 and A-865.
Loss of ubiquitination and interaction with UBQLN1; when associated with A-883.
Loss of ubiquitination and interaction with UBQLN1; when associated with A-885.
ATP-binding cassette sub-family A member 9
Abolishes binding to phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
Loss of tocopherol secretion (in vivo). No effect on tocopherol binding and intermembrane transfer (in vitro).
Calcium uniporter protein, mitochondrial
Strongly reduces calcium channel activity; when associated with V-256.
Strongly reduces calcium channel activity; when associated with W-251.
Abolishes sumoylation. No effect on nuclear body location.
In isoform 2, isoform 3, isoform 4 and isoform 6.
BTB/POZ domain-containing protein KCTD21
28S ribosomal protein S22, mitochondrial
Replication protein A 70 kDa DNA-binding subunit
Almost abolishes MCM complex DNA helicase activity. Strongly decreases MCM complex ATPase activity. No effect on MCM complex formation. No effect on ATP and ssDNA binding.
Strongly decreases MCM complex ATPase and DNA helicase activities. No effect on MCM complex formation. No effect on ATP and ssDNA binding.
Mitochondrial import inner membrane translocase subunit TIM16
Decreased enzyme activity with N(1)-acetylspermine.
Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
Inhibits transformation by N-terminal truncated form.
Leads to cytoplasmic relocalization; when associated with 12-A--A-16.
Leads to cytoplasmic relocalization; when associated with 67-A--A-70.
Neuroepithelial cell-transforming gene 1 protein
Spindlin interactor and repressor of chromatin-binding protein
Histo-blood group ABO system transferase
Complement C1r-A subcomponent heavy chain
Complement C1r-A subcomponent light chain
Interchain (between heavy and light chains)
Motile sperm domain-containing protein 3
Fails to induce myogenic differentiation in C2C12 cells.
Nucleotide triphosphate diphosphatase NUDT15
N6,N6,N6-trimethyllysine; by METTL21A; in vitro
Kelch repeat and BTB domain-containing protein 11
Voltage-gated potassium channel subunit beta-2
Ubiquitin carboxyl-terminal hydrolase 26
Rab3 GTPase-activating protein catalytic subunit
Ig-like V-type 1; required for binding to polymeric IgA and IgM
Transcription elongation factor A N-terminal and central domain-containing protein
Membrane-bound transcription factor site-2 protease
Decreased sumoylation; when associated with E-361; E-573; E-664; E-935 and E-1013.
Decreased sumoylation; when associated with E-361; E-573; E-664; E-935 and E-961.
Decreased sumoylation; when associated with E-361; E-573; E-664; E-961 and E-1013.
Decreased sumoylation; when associated with E-361; E-573; E-935; E-961 and E-1013.
Decreased sumoylation; when associated with E-361; E-664; E-935; E-961 and E-1013.
Decreased sumoylation; when associated with E-573; E-664; E-935; E-961 and E-1013.
Cyclic AMP-responsive element-binding protein 5
Recombining binding protein suppressor of hairless-like protein
Does not induce any change in single channel conductance or variance in open current levels.
Abolishes LPC transport. Abolishes LPC transport; when associated with A-92.
Significant reduction in LPC transport. Abolishes LPC transport; when associated with A-96.
Sodium-dependent lysophosphatidylcholine symporter 1
Ubiquitin carboxyl-terminal hydrolase 11
Stress-associated endoplasmic reticulum protein 2
Adhesion G protein-coupled receptor E5 subunit alpha
Adhesion G protein-coupled receptor E5 subunit beta
Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
Inactive 2'-5'-oligoadenylate synthase 1D
Inhibits stress granules localization and translational repression.
Translation initiation factor IF-2, mitochondrial
CCR4-NOT transcription complex subunit 10
50% decrease of cell growth; when associated with A-167. 50% increase of cell migration; when associated with A-167.
50% decrease of cell growth; when associated with A-171. 50% increase of cell migration; when associated with A-171.
Does not significantly increase cell motility; when associated with D-171. Does not significantly decrease cell growth; when associated with A-171.
Eliminates platelet aggregation activity.
Tumor protein p53-inducible nuclear protein 1
Volume-regulated anion channel subunit LRRC8E
Ankyrin repeat domain-containing protein 13C
26S proteasome non-ATPase regulatory subunit 12
Tumor necrosis factor receptor superfamily member 5
Coiled-coil domain-containing protein 179
Sorting and assembly machinery component 50 homolog
Coiled-coil domain-containing protein 124
High affinity immunoglobulin epsilon receptor subunit alpha
Mediator of RNA polymerase II transcription subunit 12-like protein
Does not phosphorylated by MAPK14/p38-alpha-activated MAPKAPK2/MK2. Does not affect localization. Does not affect interaction with ZCCHC8.
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
Coiled-coil domain-containing protein 169
Loss of interaction with EZR, MSN and RDX and co-localization to microvilli with EZR, MSN and RDX.
Metal-response element-binding transcription factor 2
O-linked (GalNAc...) threonine; alternate
Increased enzymatic activity and changed stereoselectivity of the oxygenation reaction to produce (11R)-HPETE preferentially instead of (12R)-HPETE.
Reduced enzymatic activity and altered stereoselectivity of the oxygenation reaction.
Reduced enzymatic activity and changed stereoselectivity of the oxygenation reaction.
Leucine-rich repeat-containing protein 23
Polypeptide N-acetylgalactosaminyltransferase 17
V-type proton ATPase 116 kDa subunit a 4
39S ribosomal protein L55, mitochondrial
Probable ATP-dependent RNA helicase DDX49
Ubiquitin-associated and SH3 domain-containing protein A
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial
Potassium voltage-gated channel subfamily H member 8
Constitutively active channel that is targeted to the cell membrane.
Dileucine internalization motif; mediates AP2 complex-dependent internalization
Dileucine motif; mediates targeting to lysosomes
Intraflagellar transport protein 74 homolog
Failure to restore localization of PALS1 and PATJ to cell-cell contacts and to restore tight junction and adherens junction formation in cells where PALS1 has been knocked down.
No effect on PARD6B interaction. Prevents interaction with PATJ; when associated with G-150.
No effect on PARD6B interaction. Prevents interaction with PATJ; when associated with G-154.
Partially prevents interaction with LIN7C.
Prevents interaction with PARD6B. Does not affect localization to the tight junctions.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-699.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-686 and A-760.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-664; A-699 and A-760.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-656; A-686; A-699 and A-760.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-643; A-664; A-686; A-699 and A-760.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-574; A-656; A-664; A-686; A-699 and A-760.
Abolishes mitotic phosphorylation and decreases mitotic index as well as CDK1 activity; when associated with A-643; A-656; A-664; A-686; A-699 and A-760.
Interchain (between SU and TM chains, or C-47 with C-507); in linked form
Loss of immunosuppressive activity. No effect on fusogenic activity.
Sodium/potassium-transporting ATPase subunit alpha-2
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Eukaryotic translation initiation factor 2-alpha kinase 3
Loss of activity and autophosphorylation.
Markly decreased expression, hearing loss associated with degeneration of cochlear hair cells and spiral ganglion cells.
ATP-dependent zinc metalloprotease YME1L1
Short transient receptor potential channel 4-associated protein
Abolishes ubiquitination. Abolishes ubiquitination and induction of phosphorylation; when associated with R-209.
Mitogen-activated protein kinase kinase kinase 7
No effect on ubiquitination. No effect on induction of phosphorylation; when associated with R-34.
No effect on ubiquitination. No effect on induction of phosphorylation; when associated with R-562.
Strongly decreases ubiquitination. Abolishes ubiquitination and induction of phosphorylation; when associated with R-158.
Scavenger receptor cysteine-rich domain-containing protein SCART1-like
TLR adapter interacting with SLC15A4 on the lysosome
Absence of phosphorylation. Reduced protein degradation. Reduced self-renewal ability in ES cells. No change in FBXW4 binding. Loss of FBXW8 binding.
Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 55% lower expression of YES1.
Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 70% lower expression of YES1. Reduced DNA binding. No change in nuclear location. No change in nuclear localization. Absence of sumoylation; when associated with R-215 and R-244.
Loss of MAPK9 binding. Absence of Ser-347 phosphorylation.
Loss of self-renewal ability in iPS cells.
No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location.
No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. No loss of ability to bind SOX2. Reduced levels of CHD4 and SMARCA4 in a POU5F1 pulldown assay.
No change in DNA binding. No change in nuclear location.
No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location.
No change in DNA binding. Reduced self-renewal ability in iPS cells. No change in nuclear location.
No change in sumoylation. No change in sumoylation; when associated with R-215. Loss of sumoylation; when associated with R-118 and R-215. No change in nuclear localization; when associated with R-118 and R-215.
No change in sumoylation; when associated with R-244. Loss of sumoylation. No change in nuclear localization; when associated with R-118 and R-244.
POU domain, class 5, transcription factor 1
Reduced DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location.
5,6-dihydroxyindole-2-carboxylic acid oxidase
Abolishes glycosylation and impairs secretion.
V-set and transmembrane domain-containing protein 2A
28S ribosomal protein S18c, mitochondrial
TATA box-binding protein-associated factor RNA polymerase I subunit B
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
No effect on interaction with PI3 kinase.
In isoform 2, isoform 3, isoform 5 and isoform 7.
In isoform 3, isoform 4, isoform 5, isoform 6 and isoform 7.
In isoform 3, isoform 4, isoform 6, isoform 7 and isoform 8.
Sperm acrosome membrane-associated protein 4
Succinate-semialdehyde dehydrogenase, mitochondrial
Poly [ADP-ribose] polymerase tankyrase-1
Dimethylaniline monooxygenase [N-oxide-forming] 4
Vitamin K epoxide reductase complex subunit 1-like protein 1
Decreased MCM complex DNA helicase activity. Reduced ssDNA binding. No effect on MCM complex formation. No effect on MCM complex ATP binding and ATPase activity.
Results in unstable hexamers. Increased MCM complex DNA helicase activity.
Sodium/potassium-transporting ATPase subunit beta-2
Zinc finger CCHC domain-containing protein 2
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
Pyruvate dehydrogenase protein X component, mitochondrial
Abolishes kinase activity. Reduces tyrosine phosphorylation by 90%; when associated with F-471.
Reduces tyrosine phosphorylation by 90%; when associated with E-608.
Disintegrin and metalloproteinase domain-containing protein 33
TRAF-interacting protein with FHA domain-containing protein B
Zinc finger and BTB domain-containing protein 11
Bromodomain and WD repeat-containing protein 1
Phosphothreonine; by ROCK2 and PKC/PRKCI
Does not inhibit cell survival upon DNA damage. Not phosphorylates p53/TP53.
X-ray repair cross-complementing protein 5
Leucine-rich repeat serine/threonine-protein kinase 1
Does not affect MN9 antibody detectability.
SCP2 sterol-binding domain-containing protein 1
Knockin mutant mice show retinal morphological anomalies and altered electroretinographic responses to light stimuli.
17-beta-hydroxysteroid dehydrogenase type 1
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
No loss of its ability to regulate TBX21-dependent gene expression or its ability to interact with SMARCA4; when associated with A-1388 and A-1390.
No loss of its ability to regulate TBX21-dependent gene expression or its ability to interact with SMARCA4; when associated with A-1388 and A-1468.
No loss of its ability to regulate TBX21-dependent gene expression or its ability to interact with SMARCA4; when associated with A-1390 and A-1468.
Potassium voltage-gated channel subfamily V member 1
O-linked (Xyl...) (keratan sulfate) threonine
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H
Peptidyl-prolyl cis-trans isomerase FKBP7
Pentatricopeptide repeat-containing protein 2, mitochondrial
Strongly reduces the interaction with MLPH.
Putative ATP-dependent RNA helicase DHX57
Loss of triacylglycerol hydrolysis activity.
Patatin-like phospholipase domain-containing protein 2
40S ribosomal protein S5, N-terminally processed
N-acetylthreonine; in 40S ribosomal protein S5, N-terminally processed
Formyl peptide receptor-related sequence 4
Adipocyte plasma membrane-associated protein
Structural maintenance of chromosomes protein 1B
Transmembrane and ubiquitin-like domain-containing protein 1
Abolishes metal-binding and ability to regulate calcium current.
Induces a strong decrease in gabapentin-binding.
Interchain (between alpha-2-2 and delta-2 chains)
Voltage-dependent calcium channel subunit alpha-2-2
Voltage-dependent calcium channel subunit alpha-2/delta-2
Voltage-dependent calcium channel subunit delta-2
Strong reduction of mitochondrial Ca(2+) peaks.
Solute carrier family 2, facilitated glucose transporter member 5
In isoform 1b, isoform 1d, isoform 2b, isoform 2d, isoform 3b and isoform 3d.
In isoform 1c, isoform 1d, isoform 2c, isoform 2d, isoform 3c and isoform 3d.
In isoform 2a, isoform 2b, isoform 2c and isoform 2d.
In isoform 3a, isoform 3b, isoform 3c and isoform 3d.
No effect on interaction with histone H3.
Loss of interaction with PDZ-domain of DLG4.
Abolishes interaction with INPPL1 SAM domain.
Inhibits EFNA1-induced vascular assembly and RAC1 activation in endothelial cells. Inhibits kinase activity. Loss of binding to VAV3 and PI3-kinase p85 subunit.
Inhibits EFNA1-induced vascular assembly and RAC1 activation in endothelial cells. No significant effect on kinase activity. Loss of binding to PI3-kinase p85 subunit.
Inhibits EFNA1-induced vascular assembly and abolishes kinase activity.
Inhibits EFNA1-induced vascular assembly and kinase activity.
Loss of kinase activity and binding to VAV3.
No significant effect on kinase activity and loss of binding to VAV2 and VAV3. Inhibits EFNA1-induced vascular assembly and RAC1 activation in endothelial cells, no significant effect on kinase activity, significant reduction in phosphorylation and binding to VAV3; when associated with E-589.
No significant effect on kinase activity and loss of binding to VAV2 and VAV3. Inhibits EFNA1-induced vascular assembly and RAC1 activation in endothelial cells, no significant effect on kinase activity, significant reduction in phosphorylation and binding to VAV3; when associated with E-595.
No significant effect on kinase activity. Significant reduction in phosphorylation.
Strongly reduced binding affinity for INPPL1 SAM domain.
V-set and immunoglobulin domain-containing protein 2
Abolishes ubiquitination and antibacterial activity and results in diffuse perinuclear distribution.
Uncharacterized protein C17orf67 homolog
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
DNA-directed RNA polymerase III subunit RPC7-like
Putative sodium-coupled neutral amino acid transporter 11
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Impaired 1-alpha hydroxylase activity toward 25-hydroxyvitamin D3.
Impaired 1-alpha hydroxylase activity toward 25-hydroxyvitamin D3. Impaired heme binding.
Confers SIAH1-mediated degradation and strong SIAH1 binding.
KH domain-containing, RNA-binding, signal transduction-associated protein 3
Abolished calmodulin-dependent protein kinase activity. Abolished ability to phosphorylate STAT1.
Calcium/calmodulin-dependent protein kinase type II subunit gamma
Ubiquitin carboxyl-terminal hydrolase 17-like protein D
Decreased UCP1 activity in thermogenic respiration.
Decreased sensitivity to activation by noradrenaline in thermogenic respiration.
Mitochondrial brown fat uncoupling protein 1
No effect on UCP1 activity in thermogenic respiration.
H-2 class I histocompatibility antigen, K-B alpha chain
Decreased level of phosphorylation. Loss of phosphorylation; when associated with A-94.
Decreased level of phosphorylation. Loss of phosphorylation; when associated with A-96.
Isobutyryl-CoA dehydrogenase, mitochondrial
N-acetylvaline; in RNA-binding motif protein, X chromosome, N-terminally processed
RNA-binding motif protein, X chromosome, N-terminally processed
FERM, ARHGEF and pleckstrin domain-containing protein 1
Complement C1q tumor necrosis factor-related protein 3
Disintegrin and metalloproteinase domain-containing protein 1b
Dramatically decreased Th2 cell differentiation.
Fails to induce Th2 cytokine production.
Moderately decreased Th2 cell differentiation.
Trans-acting T-cell-specific transcription factor GATA-3
Echinoderm microtubule-associated protein-like 6
Coiled-coil domain-containing protein 178
Ankyrin repeat domain-containing protein 39
Trinucleotide repeat-containing gene 18 protein
Loss of interaction with YWHAB and defects in the completion of cytokinesis.
Loss of localization to the perinuclear region. Loss of translocation to the nucleus upon PMA stimulation.
Natural resistance-associated macrophage protein 1
In isoform 3, isoform 4, isoform 5 and isoform 6.
Complex I intermediate-associated protein 30, mitochondrial
Significant decrease in autophosphorylation.
Cilia- and flagella- associated protein 210
Probable E3 ubiquitin-protein ligase DTX2
Basic helix-loop-helix ARNT-like protein 2
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2 and SUMO3)
Cyanocobalamin reductase / alkylcobalamin dealkylase
Abolishes most of the nuclear localization and reduces transcriptional repressor activity; when associated with A-10.
Abolishes most of the nuclear localization and reduces transcriptional repressor activity; when associated with A-11.
Abrogates transcriptional repression. No change in nuclear location.
Partial loss of transcription repression. No change in nuclear location.
Mas-related G-protein coupled receptor member X1
Coiled-coil domain-containing protein 27
Cysteine-rich hydrophobic domain-containing protein 1
Armadillo repeat-containing X-linked protein 3
Spindle and kinetochore-associated protein 2
Interchain (with C-33 in TGFB1); in linked form
Transforming growth factor beta activator LRRC32
Mild muscular dystrophy phenotype of variable penetrance due to defects in axon guidance. Mislocalization to the endoplasmic reticulum.
Impairs autoinhibition; when associated with A-385. Impairs translocation to the cell membrane.
Impairs autoinhibition; when associated with A-389. Impairs translocation to the cell membrane.
Increases guanine exchange factor activity.
Protein tyrosine phosphatase domain-containing protein 1
Class E basic helix-loop-helix protein 23
Uncharacterized protein C1orf112 homolog
N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processed
Ubiquitin-conjugating enzyme E2 G1, N-terminally processed
39S ribosomal protein L17, mitochondrial
Class E basic helix-loop-helix protein 40
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1, SUMO2 and SUMO3)
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1, SUMO2 and SUMO3); alternate
Cerebellar degeneration-related protein 2
Voltage-dependent calcium channel gamma-7 subunit
In allele W26; reduced retention in the endoplasmic reticulum.
Tuberoinfundibular peptide of 39 residues
Oxysterol-binding protein-related protein 10
Lys-63-specific deubiquitinase BRCC36-like
2-fold increase in Km for CoA. Reduced affinity for CoA.
3- to 4-fold increase in Km for CoA. Reduced affinity for CoA.
4-fold increase in Km for CoA. Reduced affinity for CoA.
Loss of fatty acyl-coenzyme A diphosphatase activity.
Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-15 and E-17.
Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-7 and E-15.
Results in exclusive localization to early endosomes and no effect on ability to promote LPS-induced IL6 and CCL5 production; when associated with E-7 and E-17.
Results in relocalization from membrane to cytosol; impairs ability to promote LPS-induced IL6 and CCL5 production.
TIR domain-containing adapter molecule 2
Fasciculation and elongation protein zeta-2
Decreased acetylation, leading to reduced malonyl-CoA decarboxylase activity.
Malonyl-CoA decarboxylase, mitochondrial
Mimicks constitutive acetylation, leading to increased malonyl-CoA decarboxylase activity.
Eukaryotic translation initiation factor 2 subunit 3, Y-linked
Leucine-rich repeat and calponin homology domain-containing protein 1
Proton acceptor; for glutaminase activity
Tubulointerstitial nephritis antigen-like
Cytochrome c oxidase subunit 7A2, mitochondrial
28 kDa heat- and acid-stable phosphoprotein
Shows less organization of the circumferential actin-filament network compared to controls.
Promotes the formation of differentiated late retinal amacrine cells.
Reduces strongly phosphorylation. Reduces transactivation on the insulin promoter in glucose-stimulated insulinoma cells; when associated with A-259 and A-266.
Reduces weakly phosphorylation. Reduces strongly phosphorylation; when associated with A-162 and A-259. Reduces transactivation on the insulin promoter in glucose-stimulated insulinoma cells; when associated with A-259 and A-274.
Reduces weakly phosphorylation. Reduces strongly phosphorylation; when associated with A-162 and A-266. Reduces transactivation on the insulin promoter in glucose-stimulated insulinoma cells; when associated with A-266 and A-274.
Reduces weakly phosphorylation. Reduces strongly phosphorylation; when associated with A-259 and A-266.
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Regulator of telomere elongation helicase 1
In A1, B(A) and B(C); requires 2 nucleotide substitutions.
Tumor necrosis factor alpha-induced protein 8-like protein 2
ATP-sensitive inward rectifier potassium channel 8
Ras-GEF domain-containing family member 1C
Up-regulation of KLH18-mediated degradation; when associated with A-37 and A-39.
Up-regulation of KLH18-mediated degradation; when associated with A-37 and A-41.
Up-regulation of KLH18-mediated degradation; when associated with A-39 and A-41.
Disintegrin and metalloproteinase domain-containing protein 8
POU Class 2 homeobox-associating factor 3
Cilia- and flagella-associated protein HOATZ
Abolishes ability to stimulate ATPase activity of HSPA5/BiP.
Testis-specific serine/threonine-protein kinase 1
Spermatogenesis-associated serine-rich protein 1
Abolishes interaction with TDRD1; when associated with K-39; K-45 and K-74.
Abolishes interaction with TDRD1; when associated with K-9; K-39 and K-45.
Abolishes interaction with TDRD1; when associated with K-9; K-39 and K-74.
Abolishes interaction with TDRD1; when associated with K-9; K-45 and K-74.
In DAH mutant; leads to arrest in meiotic prophase due to a failure of transposon piRNA amplification, resulting in the marked reduction of piRNA-bound within PIWIL4.
Omega-N-methylarginine; by PRMT5; alternate
Homozygous mice display tortuous vessels aneurysm, loss of oxidase activity and die perinatally of aortic aneurysm and dissection. Heterozygous mice display thicker arterial wall with discontinuous elastic lamellae.
Rho guanine nucleotide exchange factor 10
Inhibits interaction with RHOC. Reduces homooligomerization activity. Inhibits RHOC-dependent homooligomerization activity. Abolishes ability to rescue the morphological hair bundle defect in hair cell of RIPOR2-deficient mice.
Loss of retention in the stereocilia, but does not visibly affect the stereocilia structure in the short term. Contrary to wild-type, does not rescue the morphological defects observed in hair cells of knockout mice, which include hair bundle polarity and cohesion and length of stereocilia.
Rho family-interacting cell polarization regulator 2
Zinc finger CCHC domain-containing protein 9
Abolished NAD-dependent protein deacetylase, deacylase and mono-ADP-ribosyltransferase activities.
Abolished NAD-dependent protein deacetylase, defatty-acylase and mono-ADP-ribosyltransferase activities.
Does not affect the NAD-dependent protein defatty-acylase activity. Abolished NAD-dependent protein deacetylase and mono-ADP-ribosyltransferase activities.
Does not affect the mono-ADP-ribosyltransferase activity. Abolished NAD-dependent protein deacetylase and defatty-acylase activities.
Embryonic lethality in knockin mice; mice fail to form embryonic bodies and retain pluripotent gene expression, leading to impaired embryonic stem cell differentiation. Defects are caused by a failure to deacetylate histone H3 at 'Lys-9' (H3K9).
Increased histone deacetylase activity, ability to promote double-strand break (DSB) repair, leading to increased life span; when associated with H-249 and 260-D--Q-264.
Increased histone deacetylase activity, ability to promote double-strand break (DSB) repair, leading to increased life span; when associated with K-235 and 260-D--Q-264.
Increased histone deacetylase activity, ability to promote double-strand break (DSB) repair, leading to increased life span; when associated with K-235 and H-249.
NAD-dependent protein deacylase sirtuin-6
Serine/threonine-protein phosphatase 4 regulatory subunit 4
Protein phosphatase 1 regulatory subunit 1B
Eukaryotic translation initiation factor 3 subunit C
Proline, histidine and glycine-rich protein 1
Abolishes AMPK-mediated phosphorylation; when associated with A-177.
Abolishes AMPK-mediated phosphorylation; when associated with A-538.
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Strongly reduces coactivation of RORA activity.
Zinc finger SWIM domain-containing protein 4
In L1.2.3A mutant; reduced affinity for importin-beta.
Processed sterol regulatory element-binding protein 2
Sterol regulatory element-binding protein 2
Decreased localization of SQSTM1/p62 to damaged mitochondria; when associated with R-25; R-33 and 122-R-R-123.
Decreased localization of SQSTM1/p62 to damaged mitochondria; when associated with R-25; R-33 and R-66.
Decreased localization of SQSTM1/p62 to damaged mitochondria; when associated with R-25; R-66 and 122-R-R-123.
Decreased localization of SQSTM1/p62 to damaged mitochondria; when associated with R-33; R-66 and 122-R-R-123.
No effect on localization of SQSTM1/p62 to damaged mitochondria.
Voltage-dependent anion-selective channel protein 1
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Abolishes ubiquitin phosphorylation; when associated with A-218 and A-361.
Abolishes ubiquitin phosphorylation; when associated with A-218 and A-383.
Abolishes ubiquitin phosphorylation; when associated with A-361 and A-383.
Serine/threonine-protein kinase PINK1, mitochondrial
Wilms tumor protein 1-interacting protein
Eukaryotic translation initiation factor 1A, X-chromosomal
Serine/arginine repetitive matrix protein 1
Loss of dimerization. Almost complete loss of DNA-binding. Reduced transcriptional repression activity.
No significant effect on transcriptional repression activity.
Abolished methylation; when associated with A-323.
Decreased stability of the protein, probably due inability to interact with TBL1X; when associated with R-45. Abolishes sumoylation; when associated with R-45.
Decreased stability of the protein, probably due inability to interact with TBL1X; when associated with R-71. Abolishes sumoylation; when associated with R-71.
Does not affect localization to the nucleus.
Increased stability due to impaired ubiquitination; when associated with A-254 and A-300.
Increased stability due to impaired ubiquitination; when associated with A-254 and A-327.
Increased stability due to impaired ubiquitination; when associated with A-300 and A-327.
Promotes ubiquitination and degradation by the proteasome. Abolished methylation; when associated with A-312.
Ral guanine nucleotide dissociation stimulator
Ras-related and estrogen-regulated growth inhibitor
Voltage-dependent L-type calcium channel subunit beta-4
Abolishes hydrogen sulfide production. Slightly increased basal levels of bound persulfide.
Loss of redox potential. Abolishes bound persulfide and hydrogen sulfide production.
Reduces hydrogen sulfide production by 50%. No change in basal levels of bound persulfide.
Outer dense fiber protein 3-like protein 2
Zinc finger and SCAN domain-containing protein 26
Calmodulin-regulated spectrin-associated protein 2
Abolishes extracellular secretion. Inhibits axonal growth of callosal projections when overexpressed.
Reduces extracellular secretion. No significant effect on axonal growth of callosal projections when overexpressed.
Cytidine monophosphate-N-acetylneuraminic acid hydroxylase
Dual specificity mitogen-activated protein kinase kinase 1
Results in hyperphosphorylation of the RAF-dependent sites and prolonged ERK phosphorylation.
Results in hypophosphorylation of the RAF-dependent sites and faster ERK inactivation.
Abolished ability to activate pyroptosis, necroptosis and apoptosis (PANoptosis) in response to influenza A virus (IAV) infection in knockin mice.
Does not greatly affect phosphorylation status.
In ZBP1(Zalpha1); no effect. In ZBP1(Zalpha1-Zalpha2); knockin mice are viable and develop mild skin lesions in response to influenza A virus (IAV); mice are protected from the activation of RIPK3 and MLKL as well as the cell death; when associated with 122-D--A-126.
In ZBP1(Zalpha2); abolished ability to sense influenza A virus (IAV) Z-RNAs. In ZBP1(Zalpha2); knockin mice are viable and develop mild skin lesions in response to influenza A virus (IAV). ZBP1(Zalpha2) knockin mice are protected from the activation of RIPK3 and MLKL as well as the cell death. In ZBP1(Zalpha1-Zalpha2); knockin mice are viable and develop mild skin lesions in response to influenza A virus (IAV); mice are protected from the activation of RIPK3 and MLKL as well as the cell death; when associated with 46-D--A-50.
In mRHIMA; abolished interaction with RIPK3 and subsequent necroptosis. In ZBP1(Mr1) knockin mice are viable and do not develop skin inflammation in response to lethal dose of influenza A virus (IAV).
In mRHIMB; does not affect ability to induce necroptosis.
RIP homotypic interaction motif (RHIM) 1
RIP homotypic interaction motif (RHIM) 2
Ankyrin repeat domain-containing protein SOWAHA
Transforming growth factor beta-2 proprotein
Glycerophosphodiester phosphodiesterase 1
Tartrate-resistant acid phosphatase type 5
Sodium-dependent phosphate transport protein 2B
No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production.
No loss of IL12-induced STAT4 activation. Greatly reduced IL12-induced T-cell proliferation and IFN-gamma production.
Immunoglobulin superfamily containing leucine-rich repeat protein
In knockin mouse model, decreases protein levels. Mice exhibit a short sleep phenotype. Changes the electrophysiological properties of dorsal pons neurons.
Cilia- and flagella-associated protein 299
NACHT, LRR and PYD domains-containing protein 4A
Uncharacterized aarF domain-containing protein kinase 5
Loss of enzyme activity. No effect on ligand binding.
Reduces enzyme activity by about half. Reduces enzyme activity tenfold; when associated with A-45 and A-81.
Reduces enzyme activity by about half. Reduces enzyme activity tenfold; when associated with A-67 and A-81.
Slightly reduced enzyme activity. half. Reduces enzyme activity tenfold; when associated with A-45 and A-67.
Neuroblastoma suppressor of tumorigenicity 1
Cytosolic Fe-S cluster assembly factor NUBP2
A disintegrin and metalloproteinase with thrombospondin motifs 16
Loss of plasmanylethanolamine desaturase activity. Does not affect endoplasmic reticulum membrane localization.
Strongly reduces plasmanylethanolamine desaturase activity. Does not affect endoplasmic reticulum membrane localization.
V-type proton ATPase 16 kDa proteolipid subunit c
Potassium voltage-gated channel subfamily H member 3
Abolishes serine/threonine-protein kinase activity and ability to initiate ciliogenesis.
O-(3'-phospho-DNA)-tyrosine intermediate
Coiled-coil domain-containing protein 184
Induces resistance to 5,10-dideazatetrahydrofolate; when associated with F-48.
Induces resistance to 5,10-dideazatetrahydrofolate; when associated with G-105.
Uncharacterized protein C20orf85 homolog
24-hydroxycholesterol 7-alpha-hydroxylase
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3
Knockin new born mice are healthy and survive into adulthood without overt signs of respiratory distress. Knockin mice show a severe lung phenotype that begins with alveolar inflammatory cell infiltration at the early stage of the mouse life followed by aberrant lung remodeling with characteristics of diffuse parenchymal lung disease (DPLD)- and emphysema-like alveolar disruption in older mice.
In isoform 2, isoform 3, isoform 4 and isoform 5.
Nuclear localization. Causes nuclear relocalization of MAPK10.
In isoform 1, isoform 4, isoform 5 and isoform 7.
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
Nearly abolishes LPL transport to the lumenal surface of endothelial cells.
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
Increases localization at the cell surface when expressed in absence of PKD1.
Induces localization to the endoplasmic reticulum when expressed in absence of PKD1.
Little or no effect on calcium channel activity.
Polycystic kidney disease 2-like 1 protein
Coiled-coil domain-containing protein 38
Mothers against decapentaplegic homolog 7
No effect on stability, nuclear localization or inhibitory function in TGFB signaling. Abolishes transcriptional activity.
Uncharacterized protein C12orf60 homolog
Linked to long incubation time; requires 2 nucleotide substitutions.
Putative RNA-binding protein Luc7-like 2
Tyrosine-protein phosphatase non-receptor type 23
DNA damage-inducible transcript 4 protein
Eukaryotic translation initiation factor 1
Enhances pro-apoptotic activity; no phosphorylation.
Enhances pro-apoptotic activity; no phosphorylation; interacts with Bcl-X(L).
No pro-apoptotic activity, no interaction with Bcl-X(L).
No pro-apoptotic activity; even when associated with A-112.
Phosphoserine; by PKA, PKB, PAK1, RPS6KA1, RPS6KB1 and PKC/PRKCQ
Phosphoserine; by PKA, PKB, PIM2, PIM3, PAK1, PAK2, PAK4, PAK5, RPS6KA1 and RAF1
Abolishes AMPK-mediated phosphorylation.
Does not affect AMPK-mediated phosphorylation.
In isoform SREBP-1A-W42 and isoform SREBP-1C-W42.
In isoform SREBP-1C and isoform SREBP-1C-W42.
Processed sterol regulatory element-binding protein 1
Sterol regulatory element-binding protein 1
Strongly impairs affects phosphorylation by SIK1.
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
BCAS3 microtubule associated cell migration factor
Ubiquitin-like domain-containing CTD phosphatase 1
Advanced glycosylation end product-specific receptor
Abolishes CoA-binding. No effect on transcriptional repressor activity.
Caspase recruitment domain-containing protein 14
CUB and zona pellucida-like domain-containing protein 1
Potassium voltage-gated channel subfamily H member 5
In mlh (mergulhador phenotype); affected mice have defects in the formation of otoconia in the inner ear, but do not suffer from deafness or other inner ear defects. They cannot perceive gravity and have problems with spatial orientation and with keeping their equilibrium. They show typical head-tilting behavior and are unable to swim. Impaired subcellular localization to the plasma membrane.
In tlt (tilted phenotype); affected mice have defects in the formation of otoconia in the inner ear, but do not suffer from deafness or other inner ear defects. They cannot perceive gravity and have problems with spatial orientation and with keeping their equilibrium. They show typical head-tilting behavior and are unable to swim. Impaired subcellular localization to the plasma membrane. Effects may be due to dysregulation of pH.
cAMP-responsive element-binding protein-like 2
Golgi-associated RAB2 interactor protein 1A
Voltage-dependent P/Q-type calcium channel subunit alpha-1A
U3 small nucleolar RNA-associated protein 4 homolog
Intraflagellar transport protein 80 homolog
Actin-related protein 2/3 complex subunit 1A
FYVE, RhoGEF and PH domain-containing protein 4
Uncharacterized protein C15orf62 homolog, mitochondrial
Uncharacterized protein KIAA0825 homolog
Inactive serine/threonine-protein kinase PLK5
Induces cell cycle arrest and neurite differentiation.
Vesicular, overexpressed in cancer, prosurvival protein 1
Dehydrogenase/reductase SDR family member 7B
Adenine nucleotide translocase lysine N-methyltransferase
G_PROTEIN_RECEP_F1_2 domain-containing protein
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Strongly impairs function, tyrosine phosphorylation, subcellular location and interaction with DOK1; when associated with F-1021.
Strongly impairs function, tyrosine phosphorylation, subcellular location and interaction with DOK1; when associated with F-918.
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
No effect on carbonate dehydratase activity.
No effect on carbonate dehydratase activity. Enhanced proton transfer due to removal of the steric hindrance of F-95; when associated with A-95.
No effect on carbonate dehydratase activity. Enhanced proton transfer; when associated with A-95.
No effect on carbonate dehydratase activity. Enhanced proton transfer; when associated with H-94 or C-161.
Receptor-type tyrosine-protein phosphatase gamma
Abolishes formation of a disulfide-linked heterodimer with KCNK1.
Low affinity immunoglobulin epsilon Fc receptor
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
Microtubule-associated protein RP/EB family member 3
Spindle and kinetochore-associated protein 1
Transmembrane and ubiquitin-like domain-containing protein 2
General transcription factor IIE subunit 2
Polycystic kidney disease protein 1-like 3
Abolishes interaction with SUN1. Fails to localize at telomere.
Coiled-coil domain-containing protein 116
Rho guanine nucleotide exchange factor 16
Iroquois-class homeodomain protein IRX-2
Stromal cell-derived factor 2-like protein 1
Lysophosphatidylcholine acyltransferase 2
Abolishes interaction with RHOQ, but not the interaction with CDC42; when associated with A-136.
Abolishes interaction with RHOQ, but not the interaction with CDC42; when associated with A-139.
Does not abolish interaction with CDC42 and RHOQ.
Strongly reduces interaction with PARD3. Does not abolish interaction with CDC42 and RHOQ.
SH3 domain-binding glutamic acid-rich-like protein 2
Heterogeneous nuclear ribonucleoprotein M
N-linked (GlcNAc...) (keratan sulfate) asparagine
T-cell activation inhibitor, mitochondrial
LINE-1 type transposase domain-containing protein 1
Gamma-aminobutyric acid receptor subunit gamma-2
IxM motif; signal for cargo packaging into COPII-coated vesicles
Protein phosphatase 1 regulatory subunit 36
Eukaryotic translation initiation factor 4B
Post-GPI attachment to proteins factor 6
Abolishes interaction with MUSK and function; when associated with A-158 and A-159.
Abolishes interaction with MUSK and function; when associated with A-174.
WAP four-disulfide core domain protein 2
No effect on protein abundance. Decreased reduction by ascorbate.
No effect on protein abundance. Decreased reduction by ascorbate; when associated with A-159.
No effect on protein abundance. Decreased reduction by ascorbate; when associated with A-86.
Transmembrane ascorbate-dependent reductase CYB561
Osteoclast-associated immunoglobulin-like receptor
Transport and Golgi organization protein 6 homolog
Zinc finger MYND domain-containing protein 10
Normal mucosa of esophagus-specific gene 1 protein
Serine/arginine repetitive matrix protein 4
Coiled-coil and C2 domain-containing protein 2A
SEC14 domain and spectrin repeat-containing protein 1
Oligosaccharyltransferase complex subunit OSTC
Loss of interaction with FBXW7 and loss of CDK1-mediated phosphorylation.
Nuclear receptor subfamily 1 group D member 1
Pituitary adenylate cyclase-activating polypeptide type I receptor
Zinc finger and SCAN domain containing protein 4D
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L
Cytosolic iron-sulfur assembly component 2B
Homeodomain-interacting protein kinase 2
Inhibits localization to nuclear speckles.
No enzymatic activity, but still interacts with TP53 and NLK. Blocks the ability to induce cell growth arrest. Decreases corepressor activity.
Protein phosphatase 1 regulatory subunit 12C
Disintegrin and metalloproteinase domain-containing protein 12
Killer cell lectin-like receptor subfamily B member 1
Selection and upkeep of intraepithelial T-cells protein 5
Loss of E3 ubiquitin protein ligase activity.
Loss of interaction with EGFR and FLT3. No effect on E3 ubiquitin protein ligase activity; when associated with A-22.
Loss of interaction with EGFR and FLT3. No effect on E3 ubiquitin protein ligase activity; when associated with A-25.
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform
Rho guanine nucleotide exchange factor 38
N-alpha-acetyltransferase 16, NatA auxiliary subunit
No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-332.
No effect on overall phosphorylation. Reduces overall phosphorylation by 36%; when associated with A-339.
Zinc finger and BTB domain-containing protein 18
Accumulation in the nucleus due to defects in nuclear export.
Eukaryotic translation elongation factor 1 epsilon-1
1-acylglycerol-3-phosphate O-acyltransferase Pnpla3
Increases 1-acylglycerol-3-phosphate O-acyltransferase activity 2-fold; promotes hepatic lipid synthesis.
Loss of binding to POLR2A. Retains the ability to support transcription elongation but the resulting transcripts contain splicing defects and accumulate to high levels within the nucleus.
Interleukin-1 receptor accessory protein-like 1
Coiled-coil domain-containing protein 42
Loss of interchain disulfide bond. No effect on guidance of SLC7A5 to the plasma membrane.
Lysosomal-associated transmembrane protein 4B
Multiple epidermal growth factor-like domains protein 10
Pleckstrin homology domain-containing family H member 1
Doublesex- and mab-3-related transcription factor 1
tRNA selenocysteine 1-associated protein 1
Impairs S-nitrosylation. Abolishes S-nitrosylation; when associated with A-262.
Impairs S-nitrosylation. Abolishes S-nitrosylation; when associated with A-274.
Schiff-base intermediate with dihydroxyacetone-P
Fails to interact with DLG1. No effect on cell surface localization.
No effect on potentiation of WNT7A signaling.
G-protein coupled receptor-associated protein LMBRD2
Retinoic acid early-inducible protein 1-epsilon
Ectonucleoside triphosphate diphosphohydrolase 8
Occludin/ELL domain-containing protein 1
For hydroxyacyl-coenzyme A dehydrogenase activity
Trifunctional enzyme subunit alpha, mitochondrial
Somatomedin-B and thrombospondin type-1 domain-containing protein
Loss of inhibition of B-cell calcium mobilization.
Eukaryotic translation initiation factor 3 subunit D
Propionyl-CoA carboxylase beta chain, mitochondrial
60S ribosome subunit biogenesis protein NIP7 homolog
ATP-dependent 6-phosphofructokinase, platelet type
Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization.
Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization; when associated with S-245.
Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization; when associated with S-247.
No endosomal localization; no effect on interaction with VPS35:VPS29 dimer.
No endosomal localization; no effect on interaction with VPS35:VPS29 dimer; when associated with E-199.
No endosomal localization; no effect on interaction with VPS35:VPS29 dimer; when associated with S-197.
Vacuolar protein sorting-associated protein 26B
Abolishes interaction with ZAP70, but does not affect interaction with SLA.
Hormonally up-regulated neu tumor-associated kinase
Baculoviral IAP repeat-containing protein 5
No effect on cooperative binding with PBX1 to CYP17.
BTB/POZ domain-containing protein KCTD12
Strongly reduces transcriptional activity and interaction with NFKB1/p50 and NFKB2/p52.
Myeloid differentiation primary response protein MyD88
Prevents dimerization of death domains and activation of JNK and NF-kappa-B.
Signal peptide, CUB and EGF-like domain-containing protein 1
DNA replication complex GINS protein PSF3
Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4
Hyaluronan-binding protein 2 27 kDa light chain
Hyaluronan-binding protein 2 27 kDa light chain alternate form
Hyaluronan-binding protein 2 50 kDa heavy chain
Hyaluronan-binding protein 2 50 kDa heavy chain alternate form
GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
NAD(P)(+)--arginine ADP-ribosyltransferase
Abolishes interaction with FYN, phosphorylation by FYN, and effects on cell growth.
Phosphatidylinositol N-acetylglucosaminyltransferase subunit P
Blocks internalization from cell surface; when associated with A-24.
Blocks internalization from cell surface; when associated with A-27.
Vascular endothelial growth factor receptor 1
Decreased receptor activity in response to MTMT binding, even in the presence of copper.
Decreased receptor activity in response to MTMT binding, even in the presence of copper. Complete loss of receptor activity in the absence of copper.
Decreased receptor activity in response to MTMT binding, even in the presence of copper. Shows low constitutive activity in the presence of copper. MTMT binding in the absence of copper results in an inverse agonist effect.
No change in receptor activity in response to MTMT binding in the presence of copper. Complete loss of receptor activity in the absence of copper.
No change in receptor activity in response to MTMT binding in the presence of copper. Decreased receptor activity in the absence of copper.
No change in receptor activity in response to MTMT binding. No change in the copper-induced enhancement of receptor activity.
No receptor activity in response to MTMT binding, even in the presence of copper.
No receptor activity in response to MTMT binding, even in the presence of copper. Little to no expression detected on plasma membrane.
Calcium-activated chloride channel regulator 3A-1
WAP four-disulfide core domain protein 12
Ribonucleoside-diphosphate reductase subunit M2
Alpha-ketoglutarate-dependent dioxygenase FTO
Alters protein structure and causes an increase in whole body metabolism, leading to a lean phenotype in adult males, but not in females.
Zinc finger protein with KRAB and SCAN domains 5
Voltage-dependent T-type calcium channel subunit alpha-1G
Zinc finger CCCH domain-containing protein 15
Carcinoembryonic antigen-related cell adhesion molecule 18
Abolishes binding to CTBP1 and impairs function in transcription repression.
DNA repair protein complementing XP-A cells homolog
ATP-binding cassette sub-family A member 13
Does not affect intracellular vesicle localization. Affects cholesterol internalization.
Does not affect intracellular vesicle localization.Affects cholesterol internalization.
Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.
Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
Ras association domain-containing protein 4
Coiled-coil domain-containing protein 97
Loss of tyrosine phosphorylation. Abolishes interaction with FYN and GRB2.
Receptor-type tyrosine-protein phosphatase O
Basic leucine zipper transcriptional factor ATF-like 2
Lysosomal amino acid transporter 1 homolog
CUB and sushi domain-containing protein 3
SURP and G-patch domain-containing protein 2
Interferon-induced transmembrane protein 1
Calcium/calmodulin-dependent protein kinase II inhibitor 2
Dolichol-phosphate mannosyltransferase subunit 1
Loss of deubiquitinating activity, does not disassemble TRAF6:UBE2N ubiquitin ligase complex, abolioshes TAX1BP1 interaction with UBE2N.
Tumor necrosis factor alpha-induced protein 3
Acetyl-coenzyme A synthetase, cytoplasmic
39S ribosomal protein L47, mitochondrial
Short-chain dehydrogenase/reductase family 9C member 7
Ubiquinone biosynthesis O-methyltransferase, mitochondrial
Leucine-rich repeat-containing protein 61
Shiftless antiviral inhibitor of ribosomal frameshifting protein homolog
Transcription elongation factor A protein-like 3
Impaired DNA-binding and exonuclease activities.
Impaired exonuclease activity without affecting much the DNA-binding activity.
Meiosis-specific with OB domain-containing protein
Leucine-rich repeat-containing protein 24
Heparan sulfate glucosamine 3-O-sulfotransferase 6
Elongation of very long chain fatty acids protein 1
Transient receptor potential cation channel subfamily V member 3
Mice display deficits in anxiety as well as in repetitive and social behaviors. Decreases levels of serotonin synapses in midbrain, SLC6A4 activity and location at integrin adhesion complexes in synapses.
Calcium-binding mitochondrial carrier protein SCaMC-3
Enhanced surface expression and a lower localization to endosomal vesicles.
Membrane-associated progesterone receptor component 1
Abolishes binding of FBXL3 and SKP1. Strongly decreases CRY2 degradation.
Decreases FBXL3 binding. Strongly decreases CRY2 degradation.
Impairs protein folding. Abolishes binding of BMAL1, PER1 and FBXL3. Strongly reduces SKP1 binding.
Inhibits interaction with PER2. Does not suppress its nuclear localization. Inhibits its repression activity on CLOCK|NPAS2-BMAL1-driven transcription.
Loss of ability to inhibit CLOCK-BMAL1-mediated transcriptional activity. No loss of ability to inhibit NR1I2 transcriptional activity.
Reduced in vitro MAPK-catalyzed phosphorylation. No effect on inhibition of CLOCK-BMAL1-mediated transcriptional activity. Very little in vitro MAPK-catalyzed phosphorylation; when associated with A-265. Shorter circadian rhythm; when associated with A-553.
Reduced in vitro MAPK-catalyzed phosphorylation. No effect on inhibition of CLOCK-BMAL1-mediated transcriptional activity. Very little in vitro MAPK-catalyzed phosphorylation; when associated with A-557.
Reduced inhibition of CLOCK-BMAL1-mediated transcriptional activity. No effect on nuclear localization nor on protein stability.
Reduced interaction with NR1I2 and NR1I3. Significant decrease in interaction with NR1I2 and NR1I3; when associated with E-394 and K-397.
Reduced interaction with NR1I2 and NR1I3. Significant decrease in interaction with NR1I2 and NR1I3; when associated with E-394 and M-396.
Reduced interaction with NR1I2 and NR1I3. Significant decrease in interaction with NR1I2 and NR1I3; when associated with M-396 and K-397.
Shorter circadian rhythm; when associated with A-557.
Oncostatin-M-specific receptor subunit beta
Pulmonary surfactant-associated protein D
Cytochrome b5 domain-containing protein 1
Glycosylphosphatidylinositol anchor attachment 1 protein
Sperm-tail PG-rich repeat-containing protein 2
Impairs the ability to rescue growth in cdc25ts mutant yeasts.
Pleckstrin homology-like domain family A member 2
Granulocyte-macrophage colony-stimulating factor
Transforming growth factor beta regulator 1
Abolishes LCK, PIK3R1 and LYN binding; when associated with F-261.
Abolishes palmitoylation and lipid raft localization; when associated with S-28.
Abolishes palmitoylation and lipid raft localization; when associated with S-31.
Strongly reduces PLCG2 binding. Abolishes LCK, PIK3R1 and LYN binding; when associated with F-242.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-7
Testis-specific Y-encoded-like protein 2
Mediator of RNA polymerase II transcription subunit 18
Alpha/beta hydrolase domain-containing protein 17A
Decreased L-threonine 3-dehydrogenase activity.
Decreased L-threonine 3-dehydrogenase activity. Decreased affinity for L-threonine.
Decreased L-threonine 3-dehydrogenase activity. No effect on protein NAD(+)-binding. No gross effect on protein folding. No effect on protein stability.
L-threonine 3-dehydrogenase, mitochondrial
Constitutive coactivator of peroxisome proliferator-activated receptor gamma
Reduced catalytic activity and impaired inhibition by the R-subunit.
cAMP-dependent protein kinase catalytic subunit alpha
Isoglutamyl cysteine thioester (Cys-Gln)
Thrombospondin type-1 domain-containing protein 7B
Mitochondrial amidoxime-reducing component 1
U5 small nuclear ribonucleoprotein 40 kDa protein
Does not affect heterooligomerization with C1QL2; when associated with S-28.
Does not affect heterooligomerization with C1QL2; when associated with S-32.
Does not affect heterooligomerization with C1QL4; when associated with A-28.
Does not affect heterooligomerization with C1QL4; when associated with A-32.
SKI family transcriptional corepressor 2
CKLF-like MARVEL transmembrane domain-containing protein 4
Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 145-A--A-150.
Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 70-A--A-73.
Loss of E3 ubiquitin-protein ligase activity.
No effect on E3 ubiquitin-protein ligase activity.
Rho guanine nucleotide exchange factor 7
Zinc finger and BTB domain-containing protein 44
Potassium voltage-gated channel subfamily C member 4
EF-hand calcium-binding domain-containing protein 10
Putative tyrosine carboxypeptidase MATCAP2
Abolishes DNA-binding, abolishes transcriptional activity and estrogen-dependent NF-kappa B transcriptional repression; when associated with E-508 and E-512.
Abolishes DNA-binding, abolishes transcriptional activity and estrogen-dependent NF-kappa B transcriptional repression; when associated with E-508 and E-515.
Abolishes DNA-binding, abolishes transcriptional activity and estrogen-dependent NF-kappa B transcriptional repression; when associated with E-512 and E-515.
Abolishes estrogen binding; impairs repression of NF-kappa activity.
Abolishes estrogen-dependent NF-kappa B transcriptional repression, abolishes estrogen-induced interaction with NCOA1.
Abolishes estrogen-dependent NF-kappa B transcriptional repression, impairs transcriptional activity, abolishes estrogen-induced interaction with NCOA1.
Abolishes estrogen-dependent NF-kappa B transcriptional repression, impairs transcriptional activity, impairs estrogen-induced interaction with NCOA1; when associated with N-542 and N-548.
Abolishes estrogen-dependent NF-kappa B transcriptional repression, impairs transcriptional activity, impairs estrogen-induced interaction with NCOA1; when associated with N-542 and Q-546.
Abolishes estrogen-dependent NF-kappa B transcriptional repression, impairs transcriptional activity, impairs estrogen-induced interaction with NCOA1; when associated with Q-546 and N-548.
Abolishes estrogen-induced interaction with NCOA1.
Abolishes transcriptional activity and estrogen-induced interaction with NCOA1.
Greatly reduces transcriptional activity and estrogen-induced interaction with NCOA1.
Loss of ZDHHC7 and ZDHHC21 binding. Loss of palmitoylation.
No effect on estrogen-dependent NF-kappa B transcriptional repression, greatly impairs transcriptional activity, abolishes estrogen-induced interaction with NCOA1.
No effect on transcriptional activity and estrogen-induced interaction with NCOA1. Abolishes estrogen-induced interaction with NCOA1; when associated with A-362 and A-376.
No effect on transcriptional activity and estrogen-induced interaction with NCOA1. Abolishes estrogen-induced interaction with NCOA1; when associated with A-376 and A-380.
Reduces DNA-binding, attenuates transcriptional activity, does not effect estrogen-dependent NF-kappa B transcriptional repression; when associated with E-508 and E-512.
Reduces DNA-binding, attenuates transcriptional activity, does not effect estrogen-dependent NF-kappa B transcriptional repression; when associated with E-508 and E-515.
Reduces DNA-binding, attenuates transcriptional activity, does not effect estrogen-dependent NF-kappa B transcriptional repression; when associated with E-512 and E-515.
Reduces DNA-binding,, attenuates transcriptional activity, does not effect estrogen-dependent NF-kappa B transcriptional repression.
Reduces transcriptional activity and estrogen-induced interaction with NCOA1.
Reduces transcriptional activity, no effect on estrogen-induced interaction with NCOA1. Abolishes estrogen-induced interaction with NCOA1; when associated with A-362 and A-380.
Uncharacterized protein C12orf29 homolog
No loss of interaction with PDCD6IP. Loss of interaction with PDCD6IP; when associated with 4-A--A-5 and 47-A--A-48.
No loss of interaction with PDCD6IP. Loss of interaction with PDCD6IP; when associated with 4-A--A-5 and 51-A--A-52.
No loss of interaction with PDCD6IP. Loss of interaction with PDCD6IP; when associated with 46-A--A-47 and 50-A--A-51.
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
WLDD motif; essential for 7-methylguanosine-containing mRNA cap binding
Interphotoreceptor matrix proteoglycan 1
Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-85.
Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-87.
Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-247.
Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-250.
Protein phosphatase 1 regulatory subunit 3C
Pyridoxal-dependent decarboxylase domain-containing protein 1
Loss of function in LPA import into cell.
Loss of function in regulation of glutamatergic synaptic transmission.
No effect on localization. Decreased O-glycosylation. Loss of function in LPA internalization into cells. Loss of function in regulation of glutamatergic synaptic transmission.
Phospholipid phosphatase-related protein type 4
Heterogeneous nuclear ribonucleoprotein A0
Abolishes proteolytic cleavage and cell surface localization.
Late secretory pathway protein AVL9 homolog
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4E
Tumor protein p63-regulated gene 1 protein
Haloacid dehalogenase-like hydrolase domain-containing protein 3
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
In the samba murine model of hearing loss.
Lipoxygenase homology domain-containing protein 1
Kelch repeat and BTB domain-containing protein 8
Plasminogen activator inhibitor 1 RNA-binding protein
Heparan-alpha-glucosaminide N-acetyltransferase
Abolishes ability to suppress cell growth. Decreased phosphorylation; when associated with A-366. No change in kinase activity; when associated with A-31; A-325 and A-366.
Diminished interaction with CDKN1A and impaired ability to repair UV-induced DNA damage by affecting CDKN1A UV-induced degradation. Decreased phosphorylation; when associated with A-336. No change in kinase activity; when associated with A-31; A-325 and A-336.
Does not affect nuclear localization. Does not prevent phosphorylation at S-431.
Does not prevent S-farnesylation. Defects in neuron polarization.
Loss of kinase activity and descreased phosphorylation.
No change in kinase activity; when associated with A-31; A-336 and A-366.
No change in kinase activity; when associated with A-325; A-336 and A-366.
Phosphoserine; by autocatalysis, PKA, PKC/PRKCZ and RPS6KA1
Phosphothreonine; by ATM and autocatalysis
Abolishes ability to suppress tumorigenicity when expressed in NCI-H226 cells.
Ubiquitin carboxyl-terminal hydrolase BAP1
DNA-directed RNA polymerases I, II, and III subunit RPABC2
No effect on palmitoylation. No change in subcellular location; when associated with C-129.
No effect on palmitoylation. No change in subcellular location; when associated with G-118 and F-121.
No effect on palmitoylation. No change in subcellular location; when associated with H-135.
No effect on palmitoylation. No change in subcellular location; when associated with V-113 and F-121.
No effect on palmitoylation. No change in subcellular location; when associated with V-113 and G-118.
Reduced interaction with SYT9, but no effect on the interaction with HSC70.
Dual specificity testis-specific protein kinase 1
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
Does not inhibit interaction with ZNF473 and histone 3'-end processing.
Does not inhibit interaction with ZNF473. Reduces strongly histone 3' end processing.
Does not inhibit interaction with ZNF473. Reduces weakly histone 3' end processing.
U7 snRNA-associated Sm-like protein LSm11
Ankyrin repeat domain-containing protein 27
Disrupts interaction with RAB32 and RAB38; inhibits peripheral distribution of TYRP1 in melanocytes; no effect on dendrite formation in melanocytes.
Impairs interaction with RAB32 and RAB38.
Decreased acetylation; when associated with R-340 and Lys-369.
Decreased acetylation; when associated with R-69 and Lys-340.
Decreased acetylation; when associated with R-69 and Lys-369.
Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-572 and F-605.
Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-572 and F-643.
Decreases phosphorylation. Nearly abolishes phosphorylation; when associated with F-605 and F-643.
Leucine-rich repeat transmembrane protein FLRT1
SUMO interaction motif 1 (SIM); mediates the binding to polysumoylated substrates
V-set and immunoglobulin domain-containing protein 10
Ubiquitin carboxyl-terminal hydrolase MINDY-2
Store-operated calcium entry-associated regulatory factor
Ubiquitin-associated and SH3 domain-containing protein B
NADPH:adrenodoxin oxidoreductase, mitochondrial
Glutaminase liver isoform, mitochondrial
CB1 cannabinoid receptor-interacting protein 1
Heterogeneous nuclear ribonucleoprotein A3
Tumor necrosis factor ligand superfamily member 9
Decreased actin-binding. Loss of actin binding; when associated with 418-E-E-419 and 427-E-E-428.
Decreased actin-binding. Loss of actin binding; when associated with D-28 and 418-E-E-419.
Decreased actin-binding. Loss of actin binding; when associated with D-28 and 427-E-E-428.
VWFA and cache domain-containing protein 1
Converts the coenzyme specificity from NADP to NAD.
Fails to induce pre-BCR down-regulation, leading to splenomegaly and leukemia.
Inhibits DNA-binding activity and transcriptional activation of NEUROD1 expression.
Hydroxyacylglutathione hydrolase-like protein
Enzyme inactivation. Reduces the cleavage of PTCRA and TRAC. Does not inhibit interaction with PTCRA. Stimulates interaction with ubiquitinated proteins.
Inhibits interaction with ubiquitin and ubiquitinated proteins; when associated with A-274. Reduces the cleavage of PTCRA; when associated with A-274.
Inhibits interaction with ubiquitin and ubiquitinated proteins; when associated with A-278. Reduces the cleavage of PTCRA; when associated with A-278.
Ankyrin repeat domain-containing protein 22
Trafficking protein particle complex subunit 4
Increased cell reprogramming and pluripotency. No change in promoter-binding of target genes.
Loss of polyglutamylation, reduced cell reprogramming and pluripotency maintenance. Forms heterodimer with KLF5. No change in promoter-binding of target genes. Embryo lethality in mutant homozygous mice.
DNA-directed RNA polymerase I subunit RPA34
28S ribosomal protein S14, mitochondrial
Phosphatidylinositol-glycan biosynthesis class W protein
Reduces transcriptional activation; when associated with A-53.
Reduces transcriptional activation; when associated with A-56.
Phosphopantetheine adenylyltransferase activity abolished.
Regulatory solute carrier protein family 1 member 1
Receptor-interacting serine/threonine-protein kinase 4
Phosphoinositide 3-kinase regulatory subunit 4
Arachidonate 5-lipoxygenase-activating protein
Resistant to KLHL3-dependent protein degradation.
Multidrug resistance-associated protein 1
Sodium-dependent noradrenaline transporter
28S ribosomal protein S35, mitochondrial
Knockin mice are fertile and healthy but show increased burdens in response to bacterial infection. Cleavage by Casp7 is abolished, ceramide synthesis is decreased, leading to increased burdens in response to bacterial infection.
No activity with sphingomyelin as substrate; retains 70% of wild-type activity with bis(p-nitrophenyl) phosphate as substrate.
Retains 10% of wild-type activity with sphingomyelin as substrate; retains 70% of wild-type activity with bis(p-nitrophenyl) phosphate as substrate.
Retains 20% of wild-type activity with sphingomyelin as substrate; retains 50% of wild-type activity with bis(p-nitrophenyl) phosphate as substrate.
Retains 20% of wild-type activity with sphingomyelin as substrate; retains 70% of wild-type activity with bis(p-nitrophenyl) phosphate as substrate.
Sphingomyelin phosphodiesterase, processed form
H-2 class I histocompatibility antigen, Q10 alpha chain
About 60% loss of STAT1 phosphorylation by JAK2.
Decreased the ability of JAK2 to autophosphorylate.
More stably associated with the erythropoietin receptor complex.
Phosphorylation mimic mutant, leads to dissociation of JAK2 from the erythropoietin receptor complex.
Reduced activity in response to growth hormone.
Peroxisome proliferator-activated receptor gamma coactivator 1-beta
Leucine zipper putative tumor suppressor 3
Active breakpoint cluster region-related protein
Cysteine sulfenic acid (-SOH); alternate
Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin); alternate
Constitutively active. Disrupts general recycling of receptors in embryonic fibroblasts.
SNF-related serine/threonine-protein kinase
Misfolded, leading to the interaction with TOR1A, ubiquitination and a decrease of the half-life. Impairs intracellular transport. No effect on glycosylation.
CAP-Gly domain-containing linker protein 2
Protein farnesyltransferase subunit beta
Increases degradation via the proteasomal pathway. No effect on endoplasmic reticulum location. Impairs vitamin A uptake. No effect on retinol acyltransferase activity.
Abolished rRNA N6-adenosine-methyltransferase activity.
rRNA N6-adenosine-methyltransferase METTL5
Abolishes inhibition of NFKB2 processing; when associated with A-52.
Abolishes inhibition of NFKB2 processing; when associated with A-55.
Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)
Reduces 'Lys-48'-linked polyubiquitination; when associated with R-106.
Reduces 'Lys-48'-linked polyubiquitination; when associated with R-155.
Strongly reduces 'Lys-63'-linked ubiquitination; when associated with A-69. Abolishes inhibition of NFKB2 processing; when associated with A-67.
Strongly reduces 'Lys-63'-linked ubiquitination; when associated with A-69. Abolishes inhibition of NFKB2 processing; when associated with A-69.
PGC-1 and ERR-induced regulator in muscle protein 1
Dehydrogenase/reductase SDR family member 13
Probable mitochondrial glutathione transporter SLC25A40
CREB-regulated transcription coactivator 2
Does not affect interaction with 14-3-3 proteins.
No effect on COP1-mediated degradation of TORC1 under forksolin stimulation.
No interaction with COP1. Inhibition of degradation under exposure to FSK/INS.
Phosphoserine; by AMPK, MARK2, SIK1 and SIK2
Translocates to the nucleus and no COP1-mediated degradation.
Up-regulates CREB transcription factor activity. Reduces interaction with 14-3-3 proteins.
Up-regulates CREB-mediated gluconeogenic gene expression. No degradation of CRTC2. Loss of SIK2-mediated phosphorylation.
26S proteasome non-ATPase regulatory subunit 14
Nuclear factor of activated T-cells, cytoplasmic 4
FHF complex subunit HOOK interacting protein 2A
Ubiquitin carboxyl-terminal hydrolase 13
Confers neuron sensitivity to MPP(+)/MPTP (1-methyl-4-phenylpyridinium).
DNA-(apurinic or apyrimidinic site) endonuclease
DNA-(apurinic or apyrimidinic site) endonuclease, mitochondrial
Reduced CDK5-mediated phosphorylation. Confers neuron resistance to MPP(+)/MPTP (1-methyl-4-phenylpyridinium). Loss of CDK5-mediated phosphorylation; when associated with S-53.
Reduced CDK5-mediated phosphorylation. Loss of CDK5-mediated phosphorylation; when associated with T-232.
Cerebral cavernous malformations protein 2 homolog
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3
SPARC-related modular calcium-binding protein 2
Latent-transforming growth factor beta-binding protein 2
'Ionic lock' involved in activated form stabilization
Loss of promotion of EKLF sumoylation; when associated with G-346 and A-356.
Loss of promotion of EKLF sumoylation; when associated with G-346 and G-351.
Loss of promotion of EKLF sumoylation; when associated with G-351 and A-356.
Transcriptional and immune response regulator
Myeloid-associated differentiation marker-like protein 2
N-acetylglucosamine-1-phosphotransferase subunit alpha
N-acetylglucosamine-1-phosphotransferase subunit beta
ATP-binding cassette sub-family G member 5
Abolishes palmitoylation. No effect on function and subcellular location.
Decreases expression of both ABCG5 and ABCG8. Disrupts sterol transport activity.
Disrupts sterol transport activity. Decreases expression of both ABCG5 and ABCG8.
In trac; strongly increased levels of sitosterol, brassicasterol and campesterol in blood plasma.
Loss of one N-glycosylation site. Abolishes N-glycosylation; when associated with Q-585.
Loss of one N-glycosylation site. Abolishes N-glycosylation; when associated with Q-592.
No effect on cholesterol and sitosterol transport activity. Mildly reduced expression of ABCG5 and ABCG8.
No effect on cholesterol and sitosterol transport activity; when associated with G-196.
No effect on cholesterol and sitosterol transport activity; when associated with V-194.
Strongly decreases expression of both ABCG5 and ABCG8. Disrupts sterol transport activity.
Strongly reduces cholesterol transport activity, but has little effect on biliary secretion of campesterol and sitosterol. No effect on ATP-binding and on expression of ABCG5 and ABCG8.
General transcription and DNA repair factor IIH helicase subunit XPD
Growth hormone-inducible transmembrane protein
cAMP-dependent protein kinase type II-beta regulatory subunit
von Willebrand factor A domain-containing protein 8
Elongation of very long chain fatty acids protein 5
17-beta-hydroxysteroid dehydrogenase type 3
Na(+)/H(+) exchange regulatory cofactor NHE-RF4
Phenazine biosynthesis-like domain-containing protein 2
(E2-independent) E3 ubiquitin-conjugating enzyme FATS
Abolishes p53/TP53 polyubiquitination. No effect on interaction with p53/TP53.
No effect on p53/TP53 polyubiquitination.
Inhibits Schwann cell peripheral myelination; when associated with A-831; A-848 and A-1327.
Inhibits Schwann cell peripheral myelination; when associated with A-831; A-848 and A-881.
Inhibits Schwann cell peripheral myelination; when associated with A-831; A-881 and A-1327.
Inhibits Schwann cell peripheral myelination; when associated with A-848; A-881 and A-1327.
Strongly reduces phosphorylation by PRKCZ and abolishes interaction with PKRCI.
KH homology domain-containing protein 1B
26S proteasome non-ATPase regulatory subunit 11
Glycine N-acyltransferase-like protein Keg1
GTP:AMP phosphotransferase AK3, mitochondrial
Interchain (between alpha-2-3 and delta-3 chains)
Voltage-dependent calcium channel subunit alpha-2-3
Voltage-dependent calcium channel subunit alpha-2/delta-3
Voltage-dependent calcium channel subunit delta-3
No effect on neurite transport. Reduced axonal transport in a Madd RNAi-mediated knockdown or a Kif1b knockout background.
Reduced axon localization and aggregation in neuronal cell bodies.
Reduced interaction with NDUFS1, ROS production and apoptosis.
Fibronectin type III domain-containing protein 8
Tumor necrosis factor receptor superfamily member 11B
Potassium voltage-gated channel subfamily A member 4
Cholesterol side-chain cleavage enzyme, mitochondrial
Potassium voltage-gated channel subfamily G member 1
Proton donor; shared with dimeric partner
Promotes mono and/or diubiquitination and nuclear exclusion. Reduces phosphorylation at S-345.
Abolishes DNA-binding and transactivator/repressor activity; when associated with I-407.
Abolishes DNA-binding and transactivator/repressor activity; when associated with P-383.
Mediator of RNA polymerase II transcription subunit 9
Ras association domain-containing protein 7
Sterile alpha motif domain-containing protein 1
Gamma-secretase-activating protein 16 kDa C-terminal form
Cyclin-dependent kinase 2-associated protein 2
Solute carrier organic anion transporter family member 1A4
High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A
Membrane-bound transcription factor site-1 protease
Impaired ability to mediate deubiquitination of CGAS.
Ubiquitin carboxyl-terminal hydrolase 29
Abolishes interaction with BRCA2 and location to double-strand breaks in chromosomes.
Mutant females show reduced fertility with smaller litter sizes. Produces DNA repair defects during meiotic prophase I. Reduces loading of BRME1 and HSF2BP at the recombination nodules. Males show a slight non-significant reduction in fertility. No effect on interaction with BRCA2 and BRME1. No effect on subcellular location. Reduces BRME1 expression.
Tumor necrosis factor receptor superfamily member EDAR
In lzme; causes arrest of embryonic development during gastrulation due to chondroitin sulfate and heparan sulfate deficiency, impaired FGF signaling and impaired migration of mesoderm and endoderm.
2-fold decrease in rates of factor Xa inhibition.
400-fold decrease in rates of factor Xa inhibition.
5-fold decrease in rates of factor Xa inhibition.
50-fold decrease in rates of factor Xa inhibition.
No change in rates of factor Xa inhibition.
Transmembrane and coiled-coil domain-containing protein 5A
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
Impairs induction of SELP in activated platelets.
Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-431)
Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-423)
RNA-binding protein with multiple splicing
Cytochrome c oxidase assembly factor 6 homolog
Complement C1s-A subcomponent heavy chain
Complement C1s-A subcomponent light chain
Isopentenyl-diphosphate Delta-isomerase 1
Ankyrin repeat domain-containing protein 33B
Guided entry of tail-anchored proteins factor 1
V-set and transmembrane domain-containing protein 4
Associates more strongly with SYK. Increases calcium response upon BCR ligation.
B-cell antigen receptor complex-associated protein alpha chain
Has little effect on tyrosine phosphorylation. Reduces pre-B-cell differentiation. Abolishes binding to BLNK. Inhibits phosphorylation of BLNK. No effect on cap formation or BCR internalization. Impaired antigen presentation; when associated with F-176.
Increases tyrosine phosphorylation. Inhibits phosphorylation of BLNK. Impaired antigen presentation; when associated with F-204.
Phosphotyrosine; by SRC-type Tyr-kinases
Strongly reduces tyrosine phosphorylation and pre-B-cell differentiation; when associated with F-182. No effect on constitutive internalization of BCR.
Strongly reduces tyrosine phosphorylation and pre-B-cell differentiation; when associated with F-193. Abolishes constitutive internalization of BCR.
Pseudouridylate synthase RPUSD4, mitochondrial
EGF domain-specific O-linked N-acetylglucosamine transferase
Histone-lysine N-methyltransferase SUV39H1
Decreased promotion of neurite outgrowth. No effect on homomeric interactions.
Leucine-rich repeat and fibronectin type III domain-containing protein 1
LysM and putative peptidoglycan-binding domain-containing protein 3
Conserved oligomeric Golgi complex subunit 6
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
Heterogeneous nuclear ribonucleoprotein H
Heterogeneous nuclear ribonucleoprotein H, N-terminally processed
N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein H, N-terminally processed
Decreases autophosphorylation, binding to tRNA and Sindbis virus genomic RNA and eIF-2-alpha phosphorylation in amino acid-starved cells; when associated with I-1143.
Decreases autophosphorylation, binding to tRNA and Sindbis virus genomic RNA and eIF-2-alpha phosphorylation in amino acid-starved cells; when associated with L-1142.
Inhibits autophosphorylation, eIF-2-alpha phosphorylation and antiviral activity against Sindbis virus.
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
Coiled-coil domain-containing protein 190
Guanine nucleotide-binding protein subunit alpha-11
Cytochrome c oxidase subunit 6A1, mitochondrial
Zinc finger CCCH domain-containing protein 10
Cleavage and polyadenylation specificity factor subunit 1
Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial
Does not affect phosphorylation by AMPK in vitro.
Impairs interaction with AMPK and subsequent phosphorylation by AMPK.
Impairs phosphorylation by AMPK and ability to promote autophagy; when associated with A-317.
Impairs phosphorylation by AMPK and ability to promote autophagy; when associated with A-777.
In K2R; abolished acetylation by KAT5/TIP60 and decreased kinase activity; when associated with R-162.
In K2R; abolished acetylation by KAT5/TIP60 and decreased kinase activity; when associated with R-606.
Loss of kinase activity and autophosphorylation.
NTPase KAP family P-loop domain-containing protein 1
Leucine-rich repeat-containing protein 40
Rhomboid-related protein 2, C-terminal fragment
Rhomboid-related protein 2, N-terminal fragment
Probable isocitrate dehydrogenase [NAD] gamma 2, mitochondrial
tRNA (uracil-5-)-methyltransferase homolog B
Conserved oligomeric Golgi complex subunit 5
Insulin growth factor-like family member
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
LIM and calponin homology domains-containing protein 1
von Willebrand factor D and EGF domain-containing protein
Membrane-spanning 4-domains subfamily A member 8
DNA polymerase subunit gamma-2, mitochondrial
Leucine-rich repeat-containing protein 4
U11/U12 small nuclear ribonucleoprotein 25 kDa protein
28S ribosomal protein S23, mitochondrial
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
Loss of phosphorylation. Loss of nuclear localization.
Complete loss of ubiquitination and increased radiosensitivity.
Increased genomic instability and radiosensitivity; when associated with A-137. Reduced homologous recombination. No effect on Lys-40 acetylation. Further increase in radiosensitivity; when associated with R-37.
Increased genomic instability and radiosensitivity; when associated with A-140.
Increased radiosensitivity. No effect on phosphorylation after DNA damage. No effect on Ser-140 phosphorylation, nor on TP53BP1 recruitment to DNA double-strand breaks.
No effect on phosphorylation after DNA damage, but increased radiosensitivity. Further increase in radiosensitivity; when associated with A-140.
No effect on radiosensitivity; when associated with A-10, A-14 and A-16.
No effect on radiosensitivity; when associated with A-6, A-10 and A-14.
No effect on radiosensitivity; when associated with A-6, A-10 and A-16.
No effect on radiosensitivity; when associated with A-6, A-14 and A-16.
Zinc finger and BTB domain-containing protein 7A
Microtubule-associated protein RP/EB family member 1
Decreased protein expression and stability.
Decreased protein expression and stability. Decreased embryonic stem cell self-renewal.
Decreased protein expression. Decreased embryonic stem cell self-renewal.
Decreased protein expression. No effect on protein stability.
Decreased protein stability. Decreased embryonic stem cell self-renewal.
Increased protein expression and stability.
Increased protein expression. Decreased protein stability.
Increased protein expression. No effect on protein stability.
Increases affinity for DNA and protein stability; when associated with E-112.
Increases affinity for DNA and protein stability; when associated with R-147.
Increases affinity for DNA; when associated with E-137.
Increases affinity for DNA; when associated with R-126.
No effect on protein stability. Decreased embryonic stem cell self-renewal.
UDP-N-acetylhexosamine pyrophosphorylase-like protein 1
V-type proton ATPase subunit B, brain isoform
Loss of GTPase activity. Increased fast recycling.
Loss of palmitoyltransferase activity toward DNAJC5 and SNAP25.
No effect on interaction with DNAJC5 and SNAP25.
Does not affect interaction with ATG16L1. Induces lipidation of LC3.
Bifunctional peptidase and (3S)-lysyl hydroxylase Jmjd7
G-protein coupled receptor family C group 5 member C
Abolishes CDC20 binding; when associated with Q-314.
Abolishes CDC20 binding; when associated with R-302.
DDB1- and CUL4-associated factor 12-like protein 1
UV excision repair protein RAD23 homolog B
Glycylpeptide N-tetradecanoyltransferase 2
No IGF1-induced increase in cytoplasmic localization.
No IGF1-induced increase in cytoplasmic localization. Increased transactivation activity.
Not detected in nucleus. Redistributed in cytoplasm.
Mediator of RNA polymerase II transcription subunit 21
No effect on channel activity of the complex with LRRC8A.
Volume-regulated anion channel subunit LRRC8C
Cytidine and dCMP deaminase domain-containing protein 1
Cation-independent mannose-6-phosphate receptor
Ankyrin repeat and MYND domain-containing protein 2
Programmed cell death 6-interacting protein
Protein-glucosylgalactosylhydroxylysine glucosidase
STE20-related kinase adapter protein beta
A disintegrin and metalloproteinase with thrombospondin motifs 15
Putative mitochondrial import inner membrane translocase subunit Tim8 A-B
Histone deacetylase complex subunit SAP30
KRR1 small subunit processome component homolog
Abolishes homodimerization; when associated with 515-A-A-516.
Abolishes homodimerization; when associated with A-519.
Cilia- and flagella-associated protein 69
Long-chain fatty acid transport protein 3
Leptin receptor overlapping transcript-like 1
In isoform 2, isoform 3, isoform 4, isoform 5, isoform 7, isoform 8, isoform 11 and isoform 14.
In isoform 2, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8, isoform 9, isoform 10 and isoform 11.
In isoform 2, isoform 4, isoform 7, isoform 8 and isoform 11.
In isoform 3, isoform 5, isoform 6, isoform 8, isoform 9, isoform 10, isoform 11, isoform 12, isoform 13 and isoform 14.
In isoform 4, isoform 5, isoform 6, isoform 7, isoform 8, isoform 9, isoform 10 and isoform 11.
In isoform 4, isoform 5, isoform 6, isoform 7, isoform 8, isoform 9, isoform 10, isoform 11 and isoform 12.
In isoform 5, isoform 6, isoform 9 and isoform 10.
In isoform 5, isoform 6, isoform 9, isoform 10 and isoform 12.
MAP kinase-activating death domain protein
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Impaired enzyme activity and polyuridylated RNA-binding.
Loss of enzyme activity and polyuridylated RNA-binding; when associated with A-612.
Loss of enzyme activity and polyuridylated RNA-binding; when associated with A-74.
Pleckstrin homology-like domain family B member 2
Glucose-induced degradation protein 8 homolog
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
Disheveled-associated activator of morphogenesis 1
Reduces pericentromeric localization. Abolishes pericentromeric localization; when associated with E-580.
Reduces pericentromeric localization. Abolishes pericentromeric localization; when associated with G-239.
Chromatin modification-related protein MEAF6
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
Adipocyte-related X-chromosome expressed sequence 1
Adipocyte-related X-chromosome expressed sequence 2
Abolishes interaction with ATG8 family proteins.
Probable E3 ubiquitin-protein ligase DTX3
Tumor necrosis factor receptor superfamily member 1B
Potassium voltage-gated channel subfamily D member 1
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1
FAST kinase domain-containing protein 1, mitochondrial
Abolishes sphingosine kinase activity. Decreases apoptosis induction. Abolishes sphingosine kinase activity. No effect on endoplasmic reticulum location increase upon serum starvation. Abolishes sphingosine kinase activity and decreases apoptosis induction; when associated with A-219.
Loss of nuclear location. Loss of DNA synthesis inhibition.
Strongly decreases sphingosine kinase activity. Decreases apoptosis induction. No effect on endoplasmic reticulum location increase upon serum starvation. Abolishes sphingosine kinase activity and decreases apoptosis induction; when associated with E-213.
In pug; strongly reduced enzyme activity leads to defects in proteoglycan synthesis, abnormal chondrocyte maturation, premature ossification and dwarfism with short limbs and a compressed ribcage, which may be the cause for the increased mortality before weaning.
ADP-ribosylation factor-binding protein GGA3
Lysosome-associated membrane glycoprotein 3
Soluble lamin-associated protein of 75 kDa
Neutral and basic amino acid transport protein rBAT
Methylmalonyl-CoA epimerase, mitochondrial
Uncharacterized membrane protein C3orf80 homolog
ADP-ribosylation factor-like protein 6-interacting protein 1
Fibrinogen C domain-containing protein 1
Ankyrin repeat and MYND domain-containing protein 1
Asymmetric dimethylarginine; alternate; by PRMT1
Omega-N-methylarginine; alternate; by PRMT1
Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
EF-hand calcium-binding domain-containing protein 4A
RCC1 and BTB domain-containing protein 1
C2 calcium-dependent domain-containing protein 4C
Guanine nucleotide-binding protein G(i) subunit alpha-2
Abolishes interaction with Clostridium perfringens CPE.
No effect on interaction with Clostridium perfringens CPE.
Strongly inhibits interaction with Clostridium perfringens CPE.
Strongly reduces interaction with Clostridium perfringens CPE.
Phosphatidylcholine-sterol acyltransferase
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Coiled-coil domain-containing protein R3HCC1L
Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 2
Neuronal acetylcholine receptor subunit alpha-6
Ankyrin repeat domain-containing protein 61
Mitochondrial import receptor subunit TOM40 homolog
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
Phosphothreonine; by MAPK1, MAPK3 and MAPK8
Synaptonemal complex central element protein 3
Coiled-coil domain-containing glutamate-rich protein 1
Cleavage and polyadenylation specificity factor subunit 7
Shows no significant DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to approximately 34% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells.
Shows very weak DNA binding to a Prop1 response element in gel shift assays; transcriptional activation of a luciferase reporter gene is reduced to 43% compared with that of wild-type Prop1 in transiently transfected human embryonic kidney cells.
Mitochondrial folate transporter/carrier
Catalytically inactive mutant. Homozygous knockin male mice are sterile and display meiotic arrest at the zygotene-like stage of prophase I. LINE1 retrotransposons are derepressed and piRNA biogenesis is abolished.
Isoform 2: Abolishes the suppressor of cell proliferation activity.
Coiled-coil domain-containing protein 60
Mitochondrial import inner membrane translocase subunit TIM50
Leucine-rich repeat-containing protein 52
Actin-related protein 2/3 complex subunit 2
Solute carrier family 2, facilitated glucose transporter member 12
ATP-binding cassette sub-family C member 10
Receptor-type tyrosine-protein phosphatase alpha
Greatly reduced levels of PIAS3-mediated sumoylation. Completely abolishes sumoylation; when associated with R-315 and R-322.
Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-315.
Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-322.
Probable ATP-dependent RNA helicase DDX28
Glutamate receptor ionotropic, kainate 4
Phosphothreonine; by ROCK1, ROCK2, CDC42BP, ZIPK/DAPK3 and RAF1
Protein phosphatase 1 regulatory subunit 12A
26S proteasome non-ATPase regulatory subunit 6
CCA tRNA nucleotidyltransferase 1, mitochondrial
Abolishes binding to RAR, RXR and RAR/RXR.
Reduces binding to RAR, RXR and RAR/RXR.
Carcinoembryonic antigen-related cell adhesion molecule 2
Oxidoreductase NAD-binding domain-containing protein 1
RNA transcription, translation and transport factor protein
Peptidylprolyl isomerase domain and WD repeat-containing protein 1
T-cell-interacting, activating receptor on myeloid cells protein 1
KN motif and ankyrin repeat domain-containing protein 4
Eukaryotic translation initiation factor 4 gamma 1
Ribosomal RNA processing protein 1 homolog B
FYVE and coiled-coil domain-containing protein 1
Abolishes deubiquitinase activity without affecting interaction with RNF31. Lethality at midgestation in knockin mice caused by inactivation of the LUBAC complex, leading to cell death mediated by TNFR1 and RIPK1.
In Gum(D366E) mutant; defects in embryonic angiogenesis. Weaker mutant compared to Gum(W96R) mutant.
In Gum(W96R) mutant; defects in embryonic angiogenesis. Embryos appear normal before 11.5 dpc but die between 12.5 dpc-14 dpc, probably due to defects in organization of branching vascular networks in the head and trunk.
Decreased interaction with MAP4. No effect on interaction with IFT20.
Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Mitochondrial sodium/calcium exchanger protein
In isoform 5, isoform 8, isoform 9 and isoform 10.
Serine/threonine-protein kinase receptor R3
Capable of both nicking and hairpinning.
Capable of nicking and retains some hairpinning.
Capable of nicking but defective for hairpinning.
Defects in converting nicked products to hairpins.
Deficient in both nicking and hairpin formation.
Displays lower E3 ligase activity and affects the joining step of V(D)J recombination.
Impaired cleavage (both nicking and hairpin formation).
Impaired cleavage (defective in hairpin formation).
Impairs DNA-binding and hairpin formation while maintaining some nicking activity.
Impairs DNA-binding and reduces hairpin formation without affecting nicking activity.
Impairs DNA-binding; when associated with A-443.
Impairs DNA-binding; when associated with A-445.
Loss of E3 ligase activity and affects the joining step of V(D)J recombination.
Loss of cleavage (both nicking and hairpin formation).
Loss of cleavage and strand transfer activities without affecting allelic exclusion.
Loss of cleavage and strand transfer activities.
Moderately defective with 56% of activity.
V(D)J recombination-activating protein 1
Probable ATP-dependent RNA helicase DDX31
Abolishes export from nucleus; when associated with A-67 and A-87.
Abolishes export from nucleus; when associated with A-67 and A-92.
Abolishes export from nucleus; when associated with A-87 and A-92.
Abolishes ligand-induced translocation to the nucleus.
Abolishes ligand-induced translocation to the nucleus; when associated with A-22 and A-31.
Abolishes ligand-induced translocation to the nucleus; when associated with A-22 and A-32.
Abolishes ligand-induced translocation to the nucleus; when associated with A-31 and A-32.
In strain: C57BL/6J, A/HeJ and SPRET/Ei.
In strain: SWR/J, AKR/J, RBF/DNJ and P/J.
Small conductance calcium-activated potassium channel protein 1
Insulin-like growth factor-binding protein 6
Breast cancer metastasis-suppressor 1 homolog
MORC family CW-type zinc finger protein 1
B-cell CLL/lymphoma 7 protein family member B
Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B
Greatly reduces MHC class I peptide tetramer binding; when associated with D-160.
Greatly reduces MHC class I peptide tetramer binding; when associated with L-162.
Greatly reduces MHC class I peptide tetramer binding; when associated with N-225.
Greatly reduces MHC class I peptide tetramer binding; when associated with P-195.
Greatly reduces MHC class I peptide tetramer binding; when associated with S-196.
Greatly reduces MHC class I peptide tetramer binding; when associated with T-226.
In strain: A/Sn, BALB/c, C57BL/6, C57BL/6 X BALB/c, C57BL/6 X CBA, CB-17/SCID and NZB.
In strain: C57BL/6 X BALB/c, NZB and C57BL/6.
In strain: C57BL/6 and C57BL/6 X BALB/c.
In strain: C57BL/6, C57BL/6 X BALB/c and NZB.
In strain: C57BL/6, C57BL/6 X BALB/c and NZB; requires 2 nucleotide substitutions.
Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-175 and G-197.
Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-175 and K-227.
Increases stability and improves binding to MHC I class ligand; when associated with G-175; G-197 and K-227.
Increases stability. Increases stability and improves binding to MHC I class ligand; when associated with G-121; G-197 and K-227.
No effect on MHC class I binding; when associated with S-173.
No effect on MHC class I peptide tetramer binding.
No effect on MHC class I peptide tetramer binding; when associated with D-250.
No effect on MHC class I peptide tetramer binding; when associated with N-248.
No effect on MHC class I peptide tetramer binding; when associated with Q-169.
No effect on MHC class I peptide tetramer binding; when associated with S-174.
No effect on MHC class I peptide tetramer binding; when associated with T-170.
Selection and upkeep of intraepithelial T-cells protein 11
tRNA modification GTPase GTPBP3, mitochondrial
5'-AMP-activated protein kinase subunit beta-1
A disintegrin and metalloproteinase with thrombospondin motifs 7
DRY motif; important for ligand-induced conformation changes
No loss of binding to SH2 domain of RASGAP or NCK. Complete loss of binding to SH2 domain of RASGAP; when associated with F-304.
No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of NCK.
No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of RASGAP; when associated with F-276.
Secretory carrier-associated membrane protein 4
General transcription factor II-I repeat domain-containing protein 1
In isoform 1, isoform 3, isoform 7, isoform 9 and isoform 10.
In isoform 3, isoform 4, isoform 8 and isoform 10.
Abolishes amino-acid binding without affecting interaction with the translating ribosome.
Abolishes pyruvate kinase activity without affecting interaction with the ribosome.
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Rho guanine nucleotide exchange factor 25
Chymotrypsin-like elastase family member 1
Ras association domain-containing protein 5
Reduced specificity for HRAS and diminished discrimination between HRAS and RAP1A.
Very strong reduction of the interaction with HRAS.
Transcription cofactor vestigial-like protein 4
Interleukin-36 receptor antagonist protein
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
Cytoplasmic dynein 2 light intermediate chain 1
Glutaredoxin-like protein C5orf63 homolog
Phytanoyl-CoA dioxygenase domain-containing protein 1
Cilia- and flagella-associated protein 276
Knockin mice develop peripheral neuropathy that mimic the phenotype of intermediate forms of Charcot-Marie-Tooth disease. Knockin mice shown impairments in motor and neuromuscular functions, and aberrant myelination and axonal phenotypes.
MEF2-activating motif and SAP domain-containing transcriptional regulator
NFX1-type zinc finger-containing protein 1
Double-strand break repair protein MRE11
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
Nucleolar MIF4G domain-containing protein 1
Required for efficient binding to PPP1CA and for targeting PPP1CA to the nucleolus
SH3 domain-containing kinase-binding protein 1
NAD(P)H pyrophosphatase NUDT13, mitochondrial
Pleckstrin homology domain-containing family A member 3
Phosphoinositide-3-kinase-interacting protein 1
Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase
Phenylalanine--tRNA ligase, mitochondrial
Alpha/beta hydrolase domain-containing protein 17C
Calcium-binding and coiled-coil domain-containing protein 2
Nonsense-mediated mRNA decay factor SMG8
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Disintegrin and metalloproteinase domain-containing protein 20-like
Disintegrin and metalloproteinase domain-containing protein 24
Cyclic nucleotide-gated cation channel alpha-3
Isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial
LLGL scribble cell polarity complex component 2
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)
Attenuates activation by MAPK1/ERK1 and MAPK3/ERK2.
Loss of interaction with PDPK1 and phosphorylation at S-227.
Loss of phosphorylation and activation by PDPK1.
Loss of phosphorylation by PDPK1; loss of activation by PDPK1 and EGF.
Phosphoserine; by autocatalysis and MAPKAPK2
Vitelline membrane outer layer protein 1 homolog
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Activity-dependent neuroprotector homeobox protein 2
Pre-B-cell leukemia transcription factor 3
Actin, alpha skeletal muscle, intermediate form
EF-hand calcium-binding domain-containing protein 14
Sodium/potassium-transporting ATPase subunit beta-3
Peptidyl-prolyl cis-trans isomerase FKBP11
Aldehyde dehydrogenase family 1 member A3
Cyclic AMP-responsive element-binding protein 3-like protein 3
Processed cyclic AMP-responsive element-binding protein 3-like protein 3
Potassium voltage-gated channel subfamily KQT member 1
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4
Does not inhibit interaction with TTI1 or TTI2. Inhibits weakly interaction with TTI1 or TTI2; when associated with E-407 or R-424. Inhibits interaction with TTI1 or TTI2; when associated with E-407 and R-424.
Does not inhibit interaction with TTI1 or TTI2. Inhibits weakly interaction with TTI1 or TTI2; when associated with Q-395 or E-407. Inhibits interaction with TTI1 or TTI2; when associated with Q-395 and E-407.
Does not inhibit interaction with TTI1 or TTI2. Inhibits weakly interaction with TTI1 or TTI2; when associated with Q-395 or R-424. Inhibits interaction with TTI1 or TTI2; when associated with Q-395 and R-424.
Telomere length regulation protein TEL2 homolog
Arylacetamide deacetylase-like 4 family member 1
Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole
Localizes to the nucleus instead of the cytoplasm.
Motile sperm domain-containing protein 1
Complete loss of PAH transport activity.
Decrease in the level of cell surface expression and transport function. 80% decrease in the level of transport activity; when associated with A-49; A-122 and A-183. Complete loss of transport function; when associated with A-49; A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402 and A-427.
Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-49; A-78; A-99; A-122; A-172; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-49; A-78; A-99; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434.
Nearly abolishes membrane tubulation activity.
Protein kinase C and casein kinase substrate in neurons protein 2
Slight decrease in affinity for membranes. Nearly abolishes membrane tubulation activity.
Slight increase in affinity for membranes. Increases membrane tubulation activity.
Cadherin EGF LAG seven-pass G-type receptor 3
Abolishes interaction with NEDD9; when associated with A-538.
Abolishes interaction with NEDD9; when associated with M-540.
D-box that targets the protein for proteasomal degradation in anaphase
B-cell scaffold protein with ankyrin repeats
Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-297.
Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-32.
Reduces ability to activate insulin and glucagon gene expression.
Transmembrane gamma-carboxyglutamic acid protein 2
Coiled-coil domain-containing protein 70
Gamma-aminobutyric acid receptor-associated protein-like 2
Biogenesis of lysosome-related organelles complex 1 subunit 5
Ubiquitin carboxyl-terminal hydrolase 33
Lymphocyte antigen 6 complex locus protein G5b
Secretory carrier-associated membrane protein 3
Phosphotyrosine; by autocatalysis, LYN and JAK2
ATP-binding cassette sub-family D member 2
39S ribosomal protein L12, mitochondrial
Catalytically inactive and substrate trapping with higher affinity for substrate.
Receptor-type tyrosine-protein phosphatase eta
Substrate trapping with much higher affinity for substrate.
Probable ATP-dependent RNA helicase DDX56
Tyrosine-protein phosphatase non-receptor type 18
Reticulon-4-interacting protein 1, mitochondrial
Eukaryotic translation initiation factor 4E-binding protein 1
V-set and transmembrane domain-containing protein 2-like protein
Post-GPI attachment to proteins factor 3
V-set and immunoglobulin domain-containing protein 8
Type-1 angiotensin II receptor-associated protein
Abolishes interaction with PTPN11/SHP-2.
In W37 spotting; impaired protein stability and loss of kinase activity.
In W41 spotting; decreased kinase activity.
In W42 spotting; loss of kinase activity and impaired internalization after exposure to KITLG/SCF.
Loss-of-function mutation abolishing ligand binding.
Mast/stem cell growth factor receptor Kit
Mice display white fur, hearing loss, anemia and mast cell deficiency, plus sterility in both males and females.
Strongly reduces affinity for MLPH and SYTL4.
Loss of interaction with GRIP1 and GRIP2.
No effect on interaction with GRIP1 and GRIP2.
O-linked (Xyl...) (chondroitin sulfate) serine
Reduced potassium ion transmembrane transport.
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
ATP-sensitive inward rectifier potassium channel 14
Abolishes E3 ubiquitin ligase activity. Impairs ubiquitination of SCAP and functional regulation of cholesterol biosynthesis. Impairs sterol-induced ubiquitination and degradation of HMGCR.
Protein mono-ADP-ribosyltransferase PARP16
Phospholipid phosphatase-related protein type 3
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
Proton acceptor; for azaheterocycle hydroxylase activity
WD repeat domain phosphoinositide-interacting protein 1
Oxidoreductase-like domain-containing protein 1
Mas-related G-protein coupled receptor member A4
Interchain (between alpha-2-4 and delta-4 chains)
Voltage-dependent calcium channel subunit alpha-2-4
Voltage-dependent calcium channel subunit alpha-2/delta-4
Voltage-dependent calcium channel subunit delta-4
Ubiquitin-conjugating enzyme E2 variant 3
Leucine-rich repeat-containing protein 18
ATP-binding cassette sub-family C member 12
Abolishes calmodulin binding, unable to potentiate synaptic transmission.
No effect on homodimerization. Abolishes homodimerization; when associated with E-444.
Reduced phosphorylation. No nuclear entry PER1, CRY1 nor CKSN1E; when associated with A-661.
Reduced phosphorylation. No nuclear entry of PER1, CRY1 nor CKSN1E; when associated with A-663.
Reduces homodimerization. Abolishes homodimerization; when associated with E-267.
Basic helix-loop-helix transcription factor scleraxis
Abolishes interaction with PEX6 and ubiquitin. Impairs interaction with PEX5.
Receptor-type tyrosine-protein phosphatase U
Loss of binding to phosphatidylinositol 3-phosphate (PI3P) lipids and loss of localization to PI3P-positive organelles. Expression of mutant protein does not rescue ITGB1 recycling deficit of ANK2 null mutant fibroblasts.
Loss of interaction with RABGAP1L. Localizes on motile vesicles, but does not cotransport with RABGAP1L. Loss of polarized transport of PI3P-positive organelles to the leading edge of the migrating fibroblasts. Expression of mutant protein does not rescue ITGB1 recycling deficit of ANK2 null mutant fibroblasts nor the impaired haptotactic response of the fibroblasts.
Loss of long-range transport of PI3P-positive organelles in embryonic fibroblasts. Expression of mutant protein in ANK2 null mutant fibroblasts partially rescues ITGB1 recycling to about 50% of the wild-type levels.
Significantly weaker interaction with RABGAP1L.
Thioredoxin-related transmembrane protein 2
Bardet-Biedl syndrome 12 protein homolog
RRK motif; mediates NuRD recruitment to telomeres
Potassium voltage-gated channel subfamily KQT member 5
Results in increased cell proliferation. Results in STAT3 phosphorylation and signaling activation.
Abolishes kinase activity and ability to regulate centrosome duplication.
Disrupts homodimerization. Positively regulates intrinsic apoptotic signaling pathway.
Does not interacts with ITPR1. Triggers apoptosis.
Increases protein expression and stability; when associated with R-25, R-160 and R-177. Increases apoptosis; when associated with R-25, R-160 and R-177.
Increases protein expression and stability; when associated with R-25, R-32 and R-160. Increases apoptosis; when associated with R-25, R-32 and R-160.
Increases protein expression and stability; when associated with R-25, R-32 and R-177. Increases apoptosis; when associated with R-25, R-32 and R-177.
Increases protein expression and stability; when associated with R-32, R-160 and R-177. Increases apoptosis; when associated with R-32, R-160 and R-177.
No change in apoptosis induction; when associated with Q-71 and Q-74.
No change in apoptosis induction; when associated with Q-71 and Q-78.
No change in apoptosis induction; when associated with Q-74 and Q-78.
Selection and upkeep of intraepithelial T-cells protein 6
Tumor necrosis factor receptor superfamily member 19
Protein phosphatase 2C-like domain-containing protein 1
Developmental pluripotency-associated protein 5A
Brain from homozygous mice shows defects in the guidance of commissural axons and nerves, without evidence of cortical cells migration defects.
General transcription factor 3C polypeptide 6
Coiled-coil domain-containing protein 142
5'(3')-deoxyribonucleotidase, cytosolic type
N-acetylthreonine; in WD repeat-containing protein 61, N-terminally processed
SKI8 subunit of superkiller complex protein
SKI8 subunit of superkiller complex protein, N-terminally processed
No effect on anion channel activity. Impairs channel selectivity, so that the channel is also permeable to Na(+) ions.
Volume-regulated anion channel subunit LRRC8A
Reduced endocytosis; when associated with F-783.
Reduced endocytosis; when associated with F-795.
Adenylyltransferase and sulfurtransferase MOCS3
Cysteine persulfide intermediate; for sulfurtransferase activity
Glycyl thioester intermediate; for adenylyltransferase activity
RanBP-type and C3HC4-type zinc finger-containing protein 1
Macrophage-stimulating protein receptor alpha chain
Macrophage-stimulating protein receptor beta chain
Reduced 14-3-3 interaction. Reduced inactivation following cAMP signaling.
Reduced inhibitory activity towards TORCs in presence and absence of cAMP signaling.
Reduced interaction with 14-3-3 proteins in response to cAMP signaling and, thus, still able to inhibit TORC activity.
Reduced interaction with 14-3-3 proteins in response to cAMP signaling and, thus, still able to inhibit TORC activity. Resistant to inhibition by cAMP signaling and, thus, still able to inhibit TORC activity; when associated with A-358 and A-484.
Reduced interaction with 14-3-3 proteins in response to cAMP signaling and, thus, still able to inhibit TORC activity. Resistant to inhibition by cAMP signaling and, thus, still able to inhibit TORC activity; when associated with A-358 and A-587.
Reduced interaction with 14-3-3 proteins in response to cAMP signaling and, thus, still able to inhibit TORC activity. Resistant to inhibition by cAMP signaling and, thus, still able to inhibit TORC activity; when associated with A-484 and A-587.
Inhibits interaction with GLI2. Decreased nuclear colocalization with GLI2. No effect on DNA-binding. Decreased Ihh-induced transcriptional activity and transcription coactivator activity with GLI2.
No effect on protein stability, inhibited sumoylation, increased transcriptional activity; when associated with R-229.
No effect on protein stability, inhibited sumoylation, increased transcriptional activity; when associated with R-258.
No effect on sumoylation; when associated with 6-A--A-9; A-237; A-243 and A-320.
No effect on sumoylation; when associated with 6-A--A-9; A-237; A-243 and A-521.
No effect on sumoylation; when associated with 6-A--A-9; A-237; A-320 and A-521.
No effect on sumoylation; when associated with 6-A--A-9; A-243; A-320 and A-521.
No effect on sumoylation; when associated with A-237; A-243; A-320 and A-521.
Uncharacterized protein C11orf87 homolog
AT-rich interactive domain-containing protein 3B
Able to partially restore the interaction with the G-205 GATA1 mutant, which is usually unable to interact with ZFPM1.
Abolishes interaction with CTBP2; it however does not abolish the corepressor activity in erythropoiesis.
Does not affect the interaction with GATA1.
Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-612 and/or A-985.
Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-718 and/or A-985.
Impairs interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-612; A-718 and/or A-985.
Slightly affects the interaction with GATA1.
Slightly affects the interaction with GATA1. Strongly impairs interaction with GATA1; when associated with A-277; A-612 and/or A-718.
Transforms the C2HC-type zinc finger into a C2H2-type, leading to abolition of interaction with GATA1.
Abolishes binding to sumoylated proteins and ubiquitination and degradation of PML; when associated with 300-A--A-303 and 326-A--A-329.
Abolishes binding to sumoylated proteins and ubiquitination and degradation of PML; when associated with 300-A--A-303 and 382-A--A-385.
Abolishes binding to sumoylated proteins and ubiquitination and degradation of PML; when associated with 326-A--A-329 and 382-A--A-385.
No effect on TGF-beta and BMP signaling.
Interleukin-1 receptor-associated kinase 4
Cancer-associated gene 1 protein homolog
Trafficking protein particle complex subunit 14
CCR4-NOT transcription complex subunit 4
Putative uncharacterized protein ZNRD1-AS1
In truncate (tc); affects homeodomain stability.
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
Target of rapamycin complex 2 subunit MAPKAP1
Reduced phosphorylation. Almost complete loss of phosphorylation; when associated with F-25 and F-373.
Reduced phosphorylation. Almost complete loss of phosphorylation; when associated with F-25 and F-508.
Reduced phosphorylation. Almost complete loss of phosphorylation; when associated with F-373 and F-508.
Mediator of RNA polymerase II transcription subunit 30
NKG2-D type II integral membrane protein
Rap guanine nucleotide exchange factor 3
Requires 2 nucleotide substitutions; in T-haplotype.
Amiloride-sensitive sodium channel subunit gamma
Zinc finger ZZ-type and EF-hand domain-containing protein 1
TRAF family member-associated NF-kappa-B activator
Greatly reduces Ca(2+) permeation and channel rectification; when associated with A-672.
Greatly reduces Ca(2+) permeation and channel rectification; when associated with A-682.
Increases protein abundance at plasma membrane. Greatly reduces channel activity.
Loss of a probable N-glycosylation site. Increased expression at the cell membrane, leading to increased ion currents.
Loss of phosphorylation but no significant effect on channel activity.
No changes in channel activation by 4-alpha-PDD or heat.
Phosphomimetic mutant which enhances channel function.
Reduces channel activation by 4-alpha-PDD and heat but not hypo-osmotic cell swelling.
Results are conflicting as to whether hypotonicity-dependent channel activity is abolished.
Strongly reduced interaction with PACSIN3.
Transient receptor potential cation channel subfamily V member 4
DNA-directed RNA polymerase III subunit RPC5
Homozygous mice have their visual function which is severely reduced, consistent with significant loss of retinal ganglion cells and degeneration of axons in the optic nerve. They also display a severe multi-systemic disease characterized by reduced lifespan (<4 months), decreased weight, dilated cardiomyopathy, extrapyramidal dysfunction and gross neuro-muscular defects.
Acetylcholinesterase collagenic tail peptide
Helical; Voltage-sensor; Name=S4 of repeat I
Helical; Voltage-sensor; Name=S4 of repeat II
Helical; Voltage-sensor; Name=S4 of repeat III
Helical; Voltage-sensor; Name=S4 of repeat IV
5-glutamyl coenzyme A thioester intermediate
Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial
CCR4-NOT transcription complex subunit 3
Spermatid perinuclear RNA-binding protein
Ewing's tumor-associated antigen 1 homolog
Zinc finger HIT domain-containing protein 2
N6-adenosine-methyltransferase subunit METTL3
Serine/threonine-protein phosphatase 4 catalytic subunit
Dual specificity mitogen-activated protein kinase kinase 4
U3 small nucleolar RNA-interacting protein 2
POU domain, class 2, transcription factor 3
Loss of function mutant which is unable to promote clathrin coated-pits formation. Binds to membrane but is unable to induce membrane tubulation. Induces the formation of large and static clathrin-coated pits.
Loss of function mutant which is unable to promote clathrin coated-pits formation. Cytosolic mutant which is unable to bind and induce membrane tubulation; when associated with E-146.
Loss of function mutant which is unable to promote clathrin coated-pits formation. Cytosolic mutant which is unable to bind and induce membrane tubulation; when associated with E-165.
Loss of function mutant which is unable to promote clathrin coated-pits formation. Cytosolic mutant; when associated with E-38.
Loss of function mutant which is unable to promote clathrin coated-pits formation. Cytosolic mutant; when associated with E-73.
Loss of interaction with EPS15 and ITSN1.
Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma
Fails to repress CD8 expression. Abolishes interaction with HDAC4 and HDAC5. No effect on homodimerization and DNA binding.
In HD; disrupts sequence-specific DNA-binding; no effect on homodimerization or interaction with HDAC4 and HDAC5.
No effect on acetylation levels. Slightly decreases acetylation levels; when associated with R-210. Slightly decreases acetylation levels; when associated with R-216.
No effect on acetylation levels. Slightly decreases acetylation levels; when associated with R-339. Decreases acetylation levels; when associated with R-210 and R-216.
Slightly decreases acetylation levels. No effect on protein stability. Slightly decreases acetylation levels; when associated with R-207. Slightly decreases acetylation levels and increases protein stability; when associated with R-210. Increases protein stability; when associated with R-339. Decreases acetylation levels; when associated with R-210 and R-343. Abolishes acetylation levels and ubiquitination and highly increases protein stability; when associated with R-210 and R-339.
Slightly decreases acetylation levels. No effect on protein stability. Slightly decreases acetylation levels; when associated with R-207. Slightly decreases acetylation levels and increases protein stability; when associated with R-216. Increases protein stability; when associated with R-339. Decreases acetylation levels; when associated with R-216 and R-343. Abolishes acetylation levels and ubiquitination and highly increases protein stability; when associated with R-216 and R-339.
Slightly decreases acetylation levels. Slightly increases protein stability. Slightly decreases acetylation levels; when associated with R-343. Increases protein stability; when associated with R-210 or R-216. Abolishes acetylation levels and ubiquitination and highly increases protein stability; when associated with R-210 and R-216.
Zinc finger and BTB domain-containing protein 7B
EF-hand and coiled-coil domain-containing protein 1
Plasma membrane calcium-transporting ATPase 1
Sperm acrosome membrane-associated protein 6
Hyaluronan and proteoglycan link protein 1
10-fold decrease in MCM3AP-binding affinity, loss of relocalization to the nucleus in the presence of MCM3AP, decreased deamination activity to about 33% of the wild-type protein.
Loss of the cytidine deaminase activity; when associated with Q-58.
Loss of the cytidine deaminase activity; when associated with R-56.
Severely decreased cytidine deaminase activity. Loss of mutagenic activity.
T-cell surface glycoprotein CD3 zeta chain
EF-hand domain-containing family member C2
Nerves from mutant mice exhibit abnormal axonal organization.
Ras-specific guanine nucleotide-releasing factor RalGPS1
No effect on acetylated histone binding.
Succinate dehydrogenase assembly factor 3, mitochondrial
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
E3 ubiquitin-protein ligase RBX1, N-terminally processed
N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed
X-linked lymphocyte-regulated protein 3A
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-480; A-483; A-588; A-591 and A-700.
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-480; A-483; A-588; A-591 and A-703.
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-480; A-483; A-588; A-700 and A-703.
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-480; A-483; A-591; A-700 and A-703.
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-480; A-588; A-591; A-700 and A-703.
Abolishes disulfide reductase activity; when associated with A-158; A-161; A-483; A-588; A-591; A-700 and A-703.
Abolishes disulfide reductase activity; when associated with A-158; A-480; A-483; A-588; A-591; A-700 and A-703.
Abolishes disulfide reductase activity; when associated with A-161; A-480; A-483; A-588; A-591; A-700 and A-703.
Testis-specific Y-encoded-like protein 4
Translocating chain-associated membrane protein 2
DNA damage-inducible transcript 3 protein
Leucine-rich repeat and fibronectin type-III domain-containing protein 4
Potassium voltage-gated channel subfamily B member 2
DNA-directed RNA polymerase III subunit RPC6
Loss of N-glycosylation. No significant effect on lipid phosphatase activity.
No effect on lipid phosphatase activity.
No significant effect on lipid phosphatase activity.
PDZ-binding; involved in localization to the apical cell membrane
Mediator of RNA polymerase II transcription subunit 24
ATP synthase F(0) complex subunit C3, mitochondrial
Inactive polypeptide N-acetylgalactosaminyltransferase-like protein 5
Ankyrin repeat and SAM domain-containing protein 3
39S ribosomal protein L19, mitochondrial
Gamma-interferon-inducible lysosomal thiol reductase
Protein tyrosine phosphatase receptor type C-associated protein
Loss of kinase activity. No effect on colocalization with microtubules.
Coiled-coil domain-containing protein 68
Coiled-coil domain-containing protein 82
Protein N-lysine methyltransferase METTL21A
Apoptosis-associated speck-like protein containing a CARD
KH homology domain-containing protein 1A
Ribosomal RNA small subunit methyltransferase NEP1
MORC family CW-type zinc finger protein 3
Glutaredoxin-related protein 5, mitochondrial
Abolishes phosphorylation and prevents activation of caspase-1 and pyroptosis in response to S.typhimurium.
Mimics phosphorylation; causes rapid macrophage pyroptosis without infection.
NLR family CARD domain-containing protein 4
Voltage-dependent anion-selective channel protein 3
Asparagine synthetase [glutamine-hydrolyzing]
Probable E3 ubiquitin-protein ligase HECTD2
Phosphatidylethanolamine-binding protein 4
Pulmonary surfactant-associated protein A
Inhibits lipid kinase activity. Mice are viable and fertile but display defective adaptive and innate immune responses Signaling defects in B-cells, T-cells, mast cells and natural killer cells. Reduced B and T-cell receptor signaling. Affects development and differentiation of B -ells. Reduced memory T-cell number. Affects B- and T-cell proliferation. Attenuates immune responses in vivo. Induces inflammatory bowel disease development. Lost TCR-induced migration and localization to antigenic tissue. Affects natural killer cell maturation and cytokine production.
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Transitional endoplasmic reticulum ATPase
Propionyl-CoA carboxylase alpha chain, mitochondrial
Serine/arginine repetitive matrix protein 2
Geranylgeranyl transferase type-2 subunit beta
Peptidyl-tRNA hydrolase ICT1, mitochondrial
FXYD domain-containing ion transport regulator 6
Nuclear envelope integral membrane protein 2
Doublesex- and mab-3-related transcription factor 3
Methionine-R-sulfoxide reductase B3, mitochondrial
X-linked retinitis pigmentosa GTPase regulator
Severely reduced transcriptional repression activity.
Strongly reduces transactivation and transforming capability.
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Loss of catalytic activity. No loss of accumulation of coat proteins on internal membranes upon overexpression of Acap1.
Probable leucine--tRNA ligase, mitochondrial
Ankyrin repeat domain-containing protein SOWAHB
Decreases sulfotransferase activity (at pH 6.27).
Decreases sulfotransferase activity (at pH 6.27). Does not affect sulfotransferase activity (at pH 7.5).
Decreases sulfotransferase activity (at pH 7.5).
Has little effect on sulfotransferase activity (at pH 7.5).
Increases sulfotransferase activity (at pH 7.5).
Loss of sulfotransferase activity (at pH 7.5).
Markedly decreases enzyme stability, both apo and ligand-bound forms. Markedly decreases sulfotransferase activity (at pH 7.5).
Ubiquitin carboxyl-terminal hydrolase 16
Cytosolic arginine sensor for mTORC1 subunit 2
Tumor necrosis factor ligand superfamily member 13
BTB/POZ domain-containing protein KCTD19
Actin filament-associated protein 1-like 2
Phosphatidylcholine:ceramide cholinephosphotransferase 1
Isochorismatase domain-containing protein 2B
Interferon-stimulated 20 kDa exonuclease-like 2
Leucine-rich repeat-containing protein 47
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Leucine-rich repeat-containing protein 9
In grizzled (gr), mice show a uniformly gray coat color, rather than the expected agouti coat color.
Major facilitator superfamily domain-containing protein 12
TPR and ankyrin repeat-containing protein 1
Mitogen-activated protein kinase kinase kinase kinase 5
NADPH-dependent 3-keto-steroid reductase Hsd3b4
Zinc finger and SCAN domain-containing protein 20
Isovaleryl-CoA dehydrogenase, mitochondrial
Serine/arginine repetitive matrix protein 3
Lysosome-associated membrane glycoprotein 5
Polypeptide N-acetylgalactosaminyltransferase 11
Uncharacterized protein C6orf132 homolog
12S rRNA N4-methylcytidine methyltransferase
Impairs the ability to resucue growth in cdc25ts mutant yeasts.
Pleckstrin homology-like domain family A member 3
HUWE1-associated protein modifying stress responses
Stress-associated endoplasmic reticulum protein 1
Echinoderm microtubule-associated protein-like 4
Vasoactive intestinal polypeptide receptor 2
Hepatocyte growth factor-like protein alpha chain
Hepatocyte growth factor-like protein beta chain
Interchain (between alpha and beta chains)
Zinc finger TRAF-type-containing protein 1
Loss of binding to AKT2 and enhanced binding to MAP3K11.
Abolishes 'Lys-48'- and 'Lys-63'-specific deubiquitinase activity. Impairs 'Lys-63'-specific deubiquitination of TRAF6 substrate.
Transcription elongation regulator 1-like protein
CDK5 regulatory subunit-associated protein 2
Reduces phosphorylation by PIM2 by 60%, and decreases the transcriptional activity of MYC.
Testicular haploid expressed gene protein-like
Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
Transcription elongation factor, mitochondrial
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
IQ motif and SEC7 domain-containing protein 1
Primary cilium assembly protein FAM149B1
Complete loss of kinase activity. Phosphorylation is restored by co-transfection with wild-type protein.
Does not affect kinase activity nor secretion to the extracellular medium.
Extracellular tyrosine-protein kinase PKDCC
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Pituitary tumor-transforming gene 1 protein-interacting protein
No effect on its ability to form homohexameric or heteromeric complexes with other CBLN family members. Increased interaction with NRXN1 and NRXN3. Total loss of N-glycosylation; when associated with Q-110.
No effect on its ability to form homohexameric or heteromeric complexes with other CBLN family members. Increased interaction with NRXN1. Total loss of N-glycosylation; when associated with Q-53.
Transcription termination factor 2, mitochondrial
Disrupts interaction with PRKCSH. Nearly abolishes enzyme activity.
Cell division cycle 7-related protein kinase
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
Loss of deacetylase activity. No effect on sulfotransferase activity.
No effect on deacetylase activity. Loss of sulfotransferase activity.
Serine/threonine-protein phosphatase 6 regulatory subunit 3
Mitogen-activated protein kinase kinase kinase 12
Abolishes transcriptional activity. Increases transcriptional repression. Disrupts induction of neurite outgrowth and expression of synaptic genes. No effect on transactivation of BCL2 expression.
POU domain, class 4, transcription factor 1
Abolished protein kinase activity and ability to activate necroptosis. Knockin mice die during embryogenesis due to constitutive Ripk1- and Casp8-dependent apoptosis. Perinatal lethality observed in Ripk1 knockout mice is rescued in knockin mice carrying this mutation.
Abolishes ability to mediate necroptosis.
Abolishes ability to mediate necroptosis. Affects interaction with MLKL; when associated with A-230 and A-236.
Abolishes kinase activity and ability to mediate necroptosis. No autophosphorylation.
In RIPK3(RHIM); abolished interaction with ZBP1 and subsequent necroptosis. Perinatal lethality observed in Ripk1 knockout mice is rescued in knockin mice carrying this mutation.
Receptor-interacting serine/threonine-protein kinase 3
Slightly affects interaction with MLKL; when associated with A-230. Affects interaction with MLKL; when associated with A-230 and A-232.
Slightly affects interaction with MLKL; when associated with A-236. Affects interaction with MLKL; when associated with A-232 and A-236.
Guanine nucleotide-binding protein G(T) subunit gamma-T1
Natural cytotoxicity triggering receptor 1
N-acetylthreonine; in NHP2-like protein 1, N-terminally processed
NHP2-like protein 1, N-terminally processed
Inactive C-alpha-formylglycine-generating enzyme 2
Uncharacterized protein C11orf52 homolog
Mitogen-activated protein kinase kinase kinase kinase 4
Intermembrane lipid transfer protein VPS13A
EP300-interacting inhibitor of differentiation 3
Sterile alpha motif domain-containing protein 3
Endoplasmic reticulum resident protein 29
Increases affinity to CDCA and transcriptional activity in response to CDCA; when associated with I-388.
Increases affinity to CDCA and transcriptional activity in response to CDCA; when associated with N-370.
Growth hormone secretagogue receptor type 1
Transcription elongation factor A N-terminal and central domain-containing protein 2
Abolishes interaction with histone H4 acetylated N-terminus; when associated with 293-AA-294.
Abolishes interaction with histone H4 acetylated N-terminus; when associated with 50-AA-51.
Abolishes interaction with histone H4 acetylated N-terminus; when associated with A-298.
Abolishes interaction with histone H4 acetylated N-terminus; when associated with A-55.
Solute carrier organic anion transporter family member 3A1
Protein arginine methyltransferase NDUFAF7, mitochondrial
Platelet-derived growth factor C, latent form
Platelet-derived growth factor C, receptor-binding form
No effect on nuclear speckled pattern localization; when associated with A-166 and A-168.
No effect on nuclear speckled pattern localization; when associated with A-166 and R-170.
No effect on nuclear speckled pattern localization; when associated with A-168 and R-170.
Type II inositol 1,4,5-trisphosphate 5-phosphatase
Potassium-transporting ATPase alpha chain 2
DNA-directed RNA polymerase II subunit RPB2
In strain: DBA/2, NZB and NZW, in vitro decreases nuclease activity against free DNA by approximately twofold and decreases activity to establish a barrier to liposomal gene transfection by eightfold.
Probable ATP-dependent RNA helicase DDX17
Peptidyl-prolyl cis-trans isomerase-like 4
Leucine-rich repeat-containing protein 25
Potassium voltage-gated channel subfamily D member 3
Antiplasmin-cleaving enzyme FAP, soluble form
Localized at the cell surface, inhibits gelatinase and dipeptidyl peptidase activities and stimulates tumor suppression activity.
Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself.
Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself; when associated with E-141.
Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself; when associated with N-185.
Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself;when associated with D-69 and R-368.
Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself;when associated with V-67 and R-368.
Changed homophilic interaction; when associated with F-326.
No effect on homophilic interaction. Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself; when associated with K-317.
No effect on homophilic interaction. Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself; when associated with R-323.
No effect on homophilic interaction. Changed homophilic interaction, being unable to interact with the wild-type protein but able to bind itself; when associated with V-67 and D-69.
No effect on homophilic interaction. Changed homophilic interaction; when associated with P-143.
Lysosomal-associated transmembrane protein 5
Interferon alpha-inducible protein 27-like protein 2B
Neurogenic locus notch homolog protein 3
von Willebrand factor A domain-containing protein 5A
Mediator of RNA polymerase II transcription subunit 8
Loss of kinase activity; when associated with T-209.
Loss of kinase activity; when associated with T-214.
MAP kinase-interacting serine/threonine-protein kinase 1
Mitochondrial import receptor subunit TOM20 homolog
Golgi-associated RAB2 interactor protein 5A
Chromodomain-helicase-DNA-binding protein 9
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
tRNA N(3)-methylcytidine methyltransferase METTL6
Queuine tRNA-ribosyltransferase catalytic subunit 1
Circadian locomoter output cycles protein kaput
Decrease in sumoylation and its transcriptional activity. Abolishes sumoylation and interaction with ESR1 and decrease in its transcriptional activity; when associated with R-67.
Decrease in sumoylation and its transcriptional activity. Abolishes sumoylation and interaction with ESR1 and decrease in its transcriptional activity; when associated with R-851.
Reduced BMAL1 binding. Abolishes transcriptional activation by the CLOCK-BMAL1 heterodimer.
Reduced BMAL1 binding. Abolishes transcriptional activation by the CLOCK-BMAL1 heterodimer. Abolishes regulation of circadian clock.
Reduced BMAL1 binding. Slightly reduced transcriptional activation by the CLOCK-BMAL1 heterodimer.
Reduces histone acetyltransferase activity; when associated with A-656 and A-658.
Reduces histone acetyltransferase activity; when associated with A-656 and A-659.
Reduces histone acetyltransferase activity; when associated with A-658 and A-659.
Reduces histone acetyltransferase activity; when associated with A-669 and A-670.
Reduces histone acetyltransferase activity; when associated with A-669 and A-672.
Reduces histone acetyltransferase activity; when associated with A-670 and A-672.
Significant decrease in transcriptional activation by the CLOCK-BMAL1 heterodimer. Significant decrease in transcriptional activation by the CLOCK-BMAL1 heterodimer, reduced nuclear localization and DNA-binding; when associated with D-38.
Significant decrease in transcriptional activation by the CLOCK-BMAL1 heterodimer. Significant decrease in transcriptional activation by the CLOCK-BMAL1 heterodimer, reduced nuclear localization and DNA-binding; when associated with D-42.
Endoplasmic reticulum membrane adapter protein XK
NACHT, LRR and PYD domains-containing protein 1b allele 2
NACHT, LRR and PYD domains-containing protein 1b, C-terminus
NACHT, LRR and PYD domains-containing protein 1b, N-terminus
In chatwo; hypomorphic mutation with reduced protein stability and impaired transcriptional corepression activity. Embryonic development arrests prior to stage E9, with pronounced convergent extention defects and defective morphogenesis of extra-embryonic tissues. The anterior-posterior axis is shortened and embryos fail to undergo gut closure. No effect on interaction with ZFP568.
Phosphoserine; by ATM and ATR and dsDNA kinase
Transcription intermediary factor 1-beta
Phosphoinositide 3-kinase regulatory subunit 6
Solute carrier organic anion transporter family member 1A1
Abolishes the TNF-induced nuclear translocation.
Cerebral cavernous malformations 2 protein-like
Causes paralysis and perinatal death in homozygous mice, apparently due to asphyxia. Mutant mice have greatly reduced and amorphous skeletal muscle.
Increased channel activity, leading to permanently increased cytoplasmic Ca(2+) levels. Increased activation by calcium, and increased ryanodine binding.
Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
LIM and senescent cell antigen-like-containing domain protein 2
No effect on protein folding and histone binding.
Zinc finger MYND domain-containing protein 11
Does not induce a nuclear accumulation in unstimulated cells.
Impaired interaction with G-actin, leading to cytoplasmic accumulation.
Impaired interaction with G-actin, leading to nuclear accumulation in unstimulated cells.
In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-112.
In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-24 and A-68.
In 123-1A: Reduced interaction with G-actin, leading to a constitutively active SRF-MRTFA complex; when associated with A-68 and A-112.
Induces a nuclear accumulation in unstimulated cells.
Induces a nuclear decrease in unstimulated cells.
Myocardin-related transcription factor A
Probable loss of catalytic activity. Loss of macropinocytosis.
Abolishes mast cell activation and homodimerization.
Leukocyte immunoglobulin-like receptor subfamily B member 4B
Intracellular hyaluronan-binding protein 4
FAD-dependent oxidoreductase domain-containing protein 1
Elongation of very long chain fatty acids protein 6
Increased kinase activity and autophosphorylation.
Loss of kinase activity but no loss of interaction with RELT.
STE20/SPS1-related proline-alanine-rich protein kinase
Axonemal dynein light intermediate polypeptide 1
Mitotic spindle assembly checkpoint protein MAD1
Ubiquitin-associated domain-containing protein 1
Impaired ability to form homodimers or oligomers; when associated with A-141.
Impaired ability to form homodimers or oligomers; when associated with A-259.
Increased susceptibility to heat-inactivation. Forms homodimers and oligomers; when associated with Q-139 or Q-257. Inactive, impaired ability to form homodimers or oligomers; when associated with Q-139 and Q-257.
Increased susceptibility to heat-inactivation. Forms homodimers and oligomers; when associated with Q-221. Inactive, impaired ability to form homodimers or oligomers; when associated with Q-139 or Q-139 and Q-221.
Increased susceptibility to heat-inactivation. Forms homodimers and oligomers; when associated with Q-221. Inactive, impaired ability to form homodimers or oligomers; when associated with Q-257 or Q-221 and Q-257.
Abolishes phosphatidylserine translocase activity, abolishes effect on neurite outgrowth.
In wl; abolishes phosphatidylserine translocase activity.
PIN2/TERF1-interacting telomerase inhibitor 1
Abolished ability to promote phosphatidylserine exposure.
Abolished ability to promote phosphatidylserine exposure. Promotes localization to the cytoplasm.
Decreased phosphorylation; does not affect the phospholipid scramblase activity.
Decreased phosphorylation; impairs the phospholipid scramblase activity.
Does not affect ability to promote phosphatidylserine exposure.
In 2DA mutant; abolished cleavage by CASP3 and ability to promote phosphatidylserine exposure.
Mimics phosphorylation status; activation of the phospholipid scramblase activity.
Mimics phosphorylation status; does not affect the phospholipid scramblase activity.
DNA-directed RNA polymerases I and III subunit RPAC2
H/ACA ribonucleoprotein complex non-core subunit NAF1
Abolishes myristoylation and membrane association.
Abolishes tyrosine phosphorylation and interactions with GRB2 and PTPN11; when associated with F-196; F-306; F-349 and F-392.
Abolishes tyrosine phosphorylation and interactions with GRB2 and PTPN11; when associated with F-196; F-306; F-349 and F-436.
Abolishes tyrosine phosphorylation and interactions with GRB2 and PTPN11; when associated with F-196; F-306; F-392 and F-436.
Abolishes tyrosine phosphorylation and interactions with GRB2 and PTPN11; when associated with F-196; F-349; F-392 and F-436.
Abolishes tyrosine phosphorylation and interactions with GRB2 and PTPN11; when associated with F-306; F-349; F-392 and F-436.
Fibroblast growth factor receptor substrate 2
Proto-oncogene tyrosine-protein kinase Src
Calcium/calmodulin-dependent protein kinase type 1B
Leucine-rich repeat and IQ domain-containing protein 4
Amyloid-beta A4 precursor protein-binding family A member 2
Aberrant protein folding and increased proteasomal degradation.
Trafficking protein particle complex subunit 2
Meiotic recombination protein REC8 homolog
Calcium-binding tyrosine phosphorylation-regulated protein
Decreased transporter activity. Does not affect localization to plasma membrane.
Decreased transporter activity. Increased accumulation of protein in ER.
Decreased transporter activity. Loss of localization to plasma membrane.
Complete loss of selectivity for calcium over monocovalent cations.
Loss of N-glycosylation; when associated with N-594.
Loss of N-glycosylation; when associated with N-601.
Partial loss of selectivity for calcium over monocovalent cations.
Cilia- and flagella-associated protein 43
L-2-hydroxyglutarate dehydrogenase, mitochondrial
RNA-binding protein with serine-rich domain 1
No change in subcellular location nor colocalization with vinculin at focal adhesions nor with N-WASP at the leading edge. Loss of cell mobility function; when associated with A-637.
No change in subcellular location nor colocalization with vinculin at focal adhesions nor with N-WASP at the leading edge. No loss of cell mobility function. when associated with A-255.
No change in subcellular location nor colocalization with vinculin at focal adhesions nor with N-WASP at the leading edge. No loss of cell mobility function. when associated with D-255.
No change in subcellular location nor colocalization with vinculin at focal adhesions nor with N-WASP at the leading edge. No loss of cell mobility function; when associated with D-637.
Gypsy retrotransposon integrase-like protein 1
Abolishes binding to HRAS and KRAS; does not affect kinase activity; displays defective development of the lymphatic vasculature, resulting in perinatal appearance of chylous ascites and is highly resistant to endogenous Ras oncogene-induced tumorigenesis; when associated with A-227.
Abolishes binding to HRAS and KRAS; does not affect kinase activity; displays defective development of the lymphatic vasculature, resulting in perinatal appearance of chylous ascites and is highly resistant to endogenous Ras oncogene-induced tumorigenesis; when associated with D-208.
Loss of kinase activity; displays early embryonic lethality at 10-11 dpc and severe defects in angiogenic sprouting and vascular remodeling. Heterozygous mice yield adult mice with markedly impaired insulin signaling and reduced activation of effector pathways such as Akt/PKB.
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Abolished ubiquitination by the BCR(KLHL25)complex, leading to imapired differentiation of inducible regulatory T (iTreg) cells; when associated with R-530 and R-536.
Abolished ubiquitination by the BCR(KLHL25)complex, leading to imapired differentiation of inducible regulatory T (iTreg) cells; when associated with R-530 and R-544.
Abolished ubiquitination by the BCR(KLHL25)complex, leading to imapired differentiation of inducible regulatory T (iTreg) cells; when associated with R-536 and R-544.
Phosphoserine; by PKA and PKB/AKT1 or PKB/AKT2
Queuine tRNA-ribosyltransferase accessory subunit 2
Transmembrane emp24 domain-containing protein 1
Strongly reduced tyrosine phosphorylation. Abolishes interaction with FHL2.
Golgi apparatus membrane protein TVP23 homolog B
Interleukin-1 receptor-associated kinase-like 2
N6,N6,N6-trimethyllysine; by METTL21A; alternate
26S proteasome non-ATPase regulatory subunit 3
Endothelial differentiation-related factor 1
Protein mono-ADP-ribosyltransferase PARP4
Phosphoserine; by PHK; in form phosphorylase A
Mediator of RNA polymerase II transcription subunit 13
Coiled-coil domain-containing protein 83
GRB2-associated and regulator of MAPK protein 2
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
Tumor necrosis factor ligand superfamily member 12, membrane form
Tumor necrosis factor ligand superfamily member 12, secreted form
The mutant protein is able to coassemble and traffic to the cell membrane.
Zinc finger CCHC domain-containing protein 14
Necessary for association with microtubule and interaction with GAPDH
Dominant negative, severely disrupts circadian rhythmicity of transcription.
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Armadillo repeat-containing X-linked protein 1
No impact on substrate affinity of SLC6A19-mediated amino acid transport.
Reduces substrate affinity of SLC6A19-mediated amino acid transport.
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Fails to interacts with AGO2. Decreases nuclear localization.
Acts as a dominant negative mutant that reduces the mitochondrial Ca(2+) peaks; when associated with A-233; A-244 and A-423.
Acts as a dominant negative mutant that reduces the mitochondrial Ca(2+) peaks; when associated with A-233; A-244 and A-434.
Acts as a dominant negative mutant that reduces the mitochondrial Ca(2+) peaks; when associated with A-233; A-423 and A-434.
Acts as a dominant negative mutant that reduces the mitochondrial Ca(2+) peaks; when associated with A-244; A-423 and A-434.
Adheres to and takes up L.monocytogenes InlA coated beads.
Increased affinity for L.monocytogenes InlA. Greatly increased affinity; when associated with P-172.
Overexpressed in colon carcinoma 1 protein homolog
Unconventional prefoldin RPB5 interactor
70% reduction in binding to YWHAZ and increased nuclear localization.
Ankyrin repeat domain-containing protein 13A
Metallo-beta-lactamase domain-containing protein 1
BMP-binding endothelial regulator protein
Abnormal subnuclear localization upon glucose accumulation.
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial
Loss of interaction with CNOT7 and CNOT8. No effect on interaction with EIF4E.
Regulating synaptic membrane exocytosis protein 1
Centriolar and ciliogenesis-associated protein HYLS1
Guanine nucleotide-binding protein G(t) subunit alpha-2
Decreased affinity for NPC2. Loss of function in cholesterol transport. No effect on subcellular location. Strongly decreased affinity for NPC2; when associated with A-421.
In Npc1-nmf164; strongly decreased protein levels. Causes abnormal lipid storage in the spleen and liver, loss of cerebellar Purkinje cells and age-dependent ataxia.
Loss of function giving rise to cholesterol accumulation in kidney, liver, lung and spleen. No effect on lysosomal location. Mice display age-dependent weight loss, neurodegeneration with loss of Purkinje cells, ataxia and premature death at a median age of 84 days.
NPC intracellular cholesterol transporter 1
Strongly decreased affinity for NPC2. No effect on lysosomal location.
Strongly decreased affinity for NPC2; when associated with 503-A-A-504.
Phosphatidylinositol polyphosphate 5-phosphatase type IV
Pleckstrin homology domain-containing family B member 2
Low-density lipoprotein receptor-related protein 2
PxLPxI/L motif 1; mediates interaction with ANKRA2
PxLPxI/L motif 2; mediates interaction with ANKRA2
Ankyrin repeat domain-containing protein 9
Important role in both nutrient sensing and binding/regulation of IMPDH2
Ubiquinone biosynthesis protein COQ9, mitochondrial
Transport and Golgi organization protein 1 homolog
Ras-associating and dilute domain-containing protein
Asymmetric dimethylarginine; alternate; by PRMT8
Omega-N-methylarginine; alternate; by PRMT8
Protein transport protein Sec61 subunit alpha isoform 2
For molybdenum cofactor biosynthesis protein C activity
Molybdenum cofactor biosynthesis protein 1
Sodium-independent sulfate anion transporter
FHF complex subunit HOOK interacting protein 2B
In wr; destabilizes and consequently reduces protein levels, as well as those of the GARP complex.
Vacuolar protein sorting-associated protein 54
NAD-dependent protein deacetylase sirtuin-3
Proton-transporting V-type ATPase complex assembly regulator TMEM9
Protein phosphatase 1 regulatory inhibitor subunit 16B
Signal-induced proliferation-associated 1-like protein 1
PxLPxI/L motif; mediates interaction with ANKRA2 and 14-3-3 proteins
Sodium-coupled monocarboxylate transporter 2
Sterile alpha motif domain-containing protein 7
Glycosyltransferase 6 domain-containing protein 1
Abolished binding to N6-methyladenosine (m6A)-containing RNAs; when associated with A-378.
Abolished binding to N6-methyladenosine (m6A)-containing RNAs; when associated with A-429.
Abolishes interaction with membranes enriched in phosphatidylinositol 3-phosphate.
Abolishes interaction with membranes enriched in phosphatidylinositol 3-phosphate. Abolishes location on endosome membranes.
Abolishes location on endosome membranes.
Membrane-spanning 4-domains subfamily A member 13
U1 small nuclear ribonucleoprotein 70 kDa
Adopts a nucleus-wide distribution; when associated with D-107.
Adopts a nucleus-wide distribution; when associated with D-109.
Heart- and neural crest derivatives-expressed protein 1
Remains exclusively in the nucleolus; when associated with A-107.
Remains exclusively in the nucleolus; when associated with A-109.
EARP and GARP complex-interacting protein 1
Impairs kinase activity, when associated with A-171.
Impairs kinase activity, when associated with A-175.
Leucine-rich repeat-containing protein 69
G2/M phase-specific E3 ubiquitin-protein ligase
Inhibition on transcriptional activation on CD13 proximal promoter in endothelial cells.
No effect on transcriptional activation on CD13 proximal promoter. Increases liver specific transactivation on the IL-4 promoter.
Loss of inhibition of SPI1-mediated transcriptional activation.
Hsp90 co-chaperone Cdc37, N-terminally processed
N-acetylvaline; in Hsp90 co-chaperone Cdc37, N-terminally processed
Carnitine O-palmitoyltransferase 2, mitochondrial
Nuclear transcription factor Y subunit beta
Ankyrin repeat domain-containing protein 34B
RUN and FYVE domain-containing protein 4
EF-hand calcium-binding domain-containing protein 11
Fibronectin type III and SPRY domain-containing protein 2
KH domain-containing, RNA-binding, signal transduction-associated protein 2
Loss of interaction with RUNX1 and loss of its ability to inhibit RUNX1 transcriptional activity and suppress TH17 lineage development. Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-219 and F-225.
Loss of phosphorylation and its ability to interact with NFATC2. Loss of its ability to suppress IL-2 and Th2 cytokine production. No loss of DNA-binding and no loss its ability to activate IFN-gamma transcription.
No effect on DNA-binding or its nuclear localization.
No loss in its ability to induce IFN-gamma.
No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-52 and A-224.
No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-52 and A-76.
No loss in its ability to induce IFN-gamma. Significant reduction in its ability to induce IFN-gamma and reduced ability to promote addition of permissive chromatin-remodeling mark H3K4Me2 to the IFNG promoter region; when associated with A-76 and A-224.
No loss of interaction with ITK and GATA3.
No loss of interaction with RUNX1. Loss of interaction with ITK and GATA3.
No loss of interaction with RUNX1. No loss in its ability to induce IFN-gamma.
Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-219 and F-304.
Significant loss of ABL1-mediated phosphorylation and loss of transcriptional activator activity; when associated with F-225 and F-304.
Significant loss of ubiquitination. Loss of phosphorylation at T-302 causing loss of its ability to interact with NFATC2. Loss its ability to activate IFN-gamma transcription due to loss of DNA-binding activity. Loss of its ability to suppress IL-2 and Th2 cytokine production. Increased protein stability. Localization seen in both the nucleus and cytoplasm. No loss of interaction with GATA3 and RELA.
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Phosphoserine; by autocatalysis and IKKB
39S ribosomal protein L10, mitochondrial
cAMP-specific 3',5'-cyclic phosphodiesterase 4C
Killer cell lectin-like receptor subfamily B member 1A
Phytanoyl-CoA hydroxylase-interacting protein
Phosphoserine; by DYRK2, GSK3-alpha, GSK3-beta and PASK
Gamma-aminobutyric acid receptor subunit alpha-2
Interferon-induced protein with tetratricopeptide repeats 2
TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
U6 snRNA-associated Sm-like protein LSm7
Sperm axonemal maintenance protein CFAP97D1
Abolishes proteolytic processing; when associated with A-177.
No effect on proteolytic processing. Abolishes proteolytic processing; when associated with G-178.
Inactive tyrosine-protein kinase transmembrane receptor ROR1
Neuronal acetylcholine receptor subunit beta-4
Solute carrier family 2, facilitated glucose transporter member 8
PAN2-PAN3 deadenylation complex catalytic subunit Pan2
Phosphoserine; by MAPKAPK2, MAPKAPK3, MAPKAPK5, PKA and PKC
Phosphoserine; by MAPKAPK2, MAPKAPK3, PKA and PKC
Sodium/potassium-transporting ATPase subunit alpha-4
Reduces interaction with SHTN1; when associated with A-1048.
Reduces interaction with SHTN1; when associated with F-1047.
U5 small nuclear ribonucleoprotein 200 kDa helicase
Sodium channel protein type 3 subunit alpha
Phosphothreonine; by MLCK and ZIPK/DAPK3
Mapk-regulated corepressor-interacting protein 1
Protein mono-ADP-ribosyltransferase PARP9
Mediates endoplasmic reticulum retention
Thioredoxin domain-containing protein 16
Uncharacterized protein C17orf50 homolog
Negatively regulates of T and B cell proliferation upon activation. Significantly increases cell surface expression on T and B cells. Impairs internalization.
Influenza virus NS1A-binding protein homolog
3-oxoacyl-[acyl-carrier-protein] reductase
Decreases interaction with SCN8A in the absence of calcium.
Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
SH3 and multiple ankyrin repeat domains protein 1
Cilia- and flagella-associated protein 206
Disrupts interaction with ANKRD27; inhibits peripheral distribution of TYRP1 in melanocytes.
Inhibits the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.
Mediator of RNA polymerase II transcription subunit 17
SH3 and cysteine-rich domain-containing protein
Abolishes ATP-binding in the helicase domain without affecting the ability to confer resistance to DNA damage.
Abolishes DNA polymerase activity and ability to confer resistance to DNA damage.
In chaos1; mice display higher frequencies of spontaneous and radiation- or mitomycin C-induced micronucleated erythrocytes.
ATPase family AAA domain-containing protein 2
Loss of phosphorylation by PKA. Reduces long-term potentiation in mossy-fiber synapses in the hippocampus but has no effect on short-term potentiation. Inhibits the effect of forskolin on synaptic transmission and inhibitory post-synaptic currents. No effect on excitatory and inhibitory synaptic transmission in the hippocampus. No effect on brain structure.
Pleckstrin homology domain-containing family F member 1
Troponin C, slow skeletal and cardiac muscles
FAST kinase domain-containing protein 5, mitochondrial
Abolished ADP:ATP antiporter activity without affecting ability to regulate mitophagy; when associated with E-244.
Abolished ADP:ATP antiporter activity without affecting ability to regulate mitophagy; when associated with E-43.
Abolished ability to regulate mitophagy.
Abolished interaction with TIMM4, thereby abolishing ability to regulate mitophagy.
Single-strand selective monofunctional uracil DNA glycosylase
HCH Motif; seals regulatory zinc-binding pocket
G patch domain and ankyrin repeat-containing protein 1
Zinc finger BED domain-containing protein 4
Sodium- and chloride-dependent transporter XTRP3B
Tubulin polyglutamylase complex subunit 2
Sphingolipid delta(4)-desaturase/C4-monooxygenase DES2
Ankyrin repeat domain-containing protein 24
Hypoxanthine-guanine phosphoribosyltransferase
Mini-chromosome maintenance complex-binding protein
Loss of affinity for GTP and loss of interaction with RUNDC3A.
Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176.
Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177.
Corticotropin-releasing factor receptor 1
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11
Segment polarity protein dishevelled homolog DVL-3
Symmetric dimethylarginine; by PRMT7; alternate
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3
Ubiquitin carboxyl-terminal hydrolase 12
Low-density lipoprotein receptor class A domain-containing protein 3
Peptidyl-prolyl cis-trans isomerase FKBP2
Coiled-coil domain-containing protein 171
Pleckstrin homology domain-containing family G member 5
Leucine-rich repeat-containing protein 1
Single-pass membrane and coiled-coil domain-containing protein 3
Torsin-1A-interacting protein 2, isoform IFRG15
Ankyrin repeat and zinc finger domain-containing protein 1
Cysteine-rich perinuclear theca protein 1
Abolishes interaction with SLC9A3R1 and effect on synapse formation.
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Folylpolyglutamate synthase, mitochondrial
Synapse differentiation-inducing gene protein 1
Loss of proline isomerization; no phenotype when introduced in mice, animals are viable and fertile with no microcephaly or defective cortical lamination; no splicing defect.
Bromodomain adjacent to zinc finger domain protein 2B
Proto-oncogene serine/threonine-protein kinase mos
Brain and acute leukemia cytoplasmic protein
Electron transfer flavoprotein subunit alpha, mitochondrial
Enhances stability and accumulates in the cytoplasm; when associated with R-231 (isoform 1).
Enhances stability and accumulates in the cytoplasm; when associated with R-252 (isoform 1).
Reduces degradation (isoform 1); when associated with 177-L-H-178.
Reduces degradation (isoform 1); when associated with 238-L-H-239.
X-box-binding protein 1, cytoplasmic form
Coiled-coil domain-containing protein 78
Abolishes interaction with PTPN6 and PTPN11. Loss of function; when associated with F-216.
Abolishes interaction with PTPN6 and PTPN11. Loss of function; when associated with F-241.
Palmitoyl-protein thioesterase ABHD10, mitochondrial
Reduced levels of autophosphorylation. Abolishes interaction with GRB2 and GRB7. Abolishes phosphorylation of GRB7 and PIK3R1.
Histone-lysine N-methyltransferase EHMT1
In LM3; does not form heterodimer with EHMT2 and is defective in mediating both H3K9me and DNA methylation.
In LM4; does not prevent methyltransferase activity.
In LM7; does not prevent methyltransferase activity; when associated with F-1240.
In LM7; does not prevent methyltransferase activity; when associated with V-1153.
Protein phosphatase 1 regulatory subunit 32
Mitochondrial intermembrane space import and assembly protein 40
Alpha/beta-tubulin-N-acetyltransferase 9
WD repeat and HMG-box DNA-binding protein 1
Sodium-dependent neutral amino acid transporter B(0)AT2
Loss of tyrosine phosphorylations by CABLES1 and ABL1; decreased activity.
Suppressor APC domain-containing protein 1
Leucine-rich repeat-containing protein 30
Serine-rich and transmembrane domain-containing protein 1
Significantly impaired guanine nucleotide exchange factor activity on ARF6 in PC12 cells.
Decreased dendritic localization and decreased translation of reporter mRNA, when associated with Ala-149.
Decreased dendritic localization and decreased translation of reporter mRNA, when associated with Ala-165.
Disrupts interaction with EIF4A and fails to stimulate translation.
In isoform 4, isoform 6, isoform 8 and isoform 9.
Loss of association with polysomes and loss of translation stimulation.
Omega-N-methylarginine; by CARM1; alternate
Reduced association with polysomes and reduced stimulation of translation.
Leucine-rich repeat and WD repeat-containing protein 1
Hydroxymethylglutaryl-CoA synthase, mitochondrial
Non-POU domain-containing octamer-binding protein
In Rex mutant M100573; mice exhibit curly hair and vibrissae. The diameter of the hair shaft is irregular due to morphological abnormalities in all three layers of the irs.
In Rex mutant Re; mice exhibit curly hair and vibrissae. The diameter of the hair shaft is irregular due to morphological abnormalities in all three layers of the irs.
Complement decay-accelerating factor transmembrane isoform
PEST proteolytic signal-containing nuclear protein
Class A basic helix-loop-helix protein 15
MAP/microtubule affinity-regulating kinase 4
Transforming acidic coiled-coil-containing protein 1
Circadian-associated transcriptional repressor
TGF-beta-activated kinase 1 and MAP3K7-binding protein 1
43 kDa receptor-associated protein of the synapse
Chloride intracellular channel protein 6
Helical; Note=After insertion into the membrane
Mannose-1-phosphate guanyltransferase alpha
Has no effect on Treg effector functions.
In isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9.
Ankyrin repeat and IBR domain-containing protein 1
Tumor necrosis factor alpha-induced protein 8
Fumarylacetoacetate hydrolase domain-containing protein 2A
Breast cancer type 1 susceptibility protein homolog
Probable inactive tRNA-specific adenosine deaminase-like protein 3
U4/U6 small nuclear ribonucleoprotein Prp4
Copper chaperone for superoxide dismutase
Isopentenyl-diphosphate delta-isomerase 2
5-demethoxyubiquinone hydroxylase, mitochondrial
EEF1AKMT4-ECE2 readthrough transcript protein
Cullin-associated NEDD8-dissociated protein 1
Leucine-rich repeat transmembrane neuronal protein 3
39S ribosomal protein L27, mitochondrial
Mitochondrial amidoxime reducing component 2
Vimentin-type intermediate filament-associated coiled-coil protein
Class E basic helix-loop-helix protein 41
ATP synthase subunit alpha, mitochondrial
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3
N-acetylated-alpha-linked acidic dipeptidase 2
Probable ATP-dependent RNA helicase DDX20
PTB-containing, cubilin and LRP1-interacting protein
Transforming growth factor-beta receptor-associated protein 1
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Phosphoserine; by MAPK1, MAPK3 and MAPK14
Phosphothreonine; by MAPK1, MAPK3 and MAPK14
Sodium channel protein type 9 subunit alpha
FERM and PDZ domain-containing protein 4
Inhibits interaction with RHOA, reduces localization at junctions and prevents apical junction formation; when associated with K-155.
Inhibits interaction with RHOA, reduces localization at junctions and prevents apical junction formation; when associated with K-60.
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)
Trinucleotide repeat-containing gene 6A protein
O-linked (Xyl...) (heparan sulfate) serine
Long-chain fatty acid transport protein 2
U11/U12 small nuclear ribonucleoprotein 35 kDa protein
Enoyl-CoA hydratase domain-containing protein 2, mitochondrial
Testicular spindle-associated protein SHCBP1L
Abolished palmitoylation, leading to impaired ability mediate synaptic long-term depression (LTD).
Abolished phosphorylation by CaMK2; knockin mice display normal baseline synaptic transmission and long-term potentiation (LTP) in the hippocampus, but metabotropic receptor-LTD is increased; when associated with A-278.
Activity-regulated cytoskeleton-associated protein
Impaired ability to homooligomerize at 30 degrees Celsius.
Phosphomimetic mutant; does not affect ability to homooligomerize.
Phosphomimetic mutant; impaired ability to homooligomerize at 30 degrees Celsius.
All-trans-retinol dehydrogenase [NAD(+)] ADH4
Strongly increases activity towards ethanol, increases KM for benzoquinone 10-fold.
Strongly increases enzyme activity, serves as proton acceptor.
Loss of N-glycosylation; 90% decrease of plasma membrane expression.
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Homozygotes are born at Mendelian ratios and have no phenotypic abnormalities from birth to adulthood. Increased ERN1 activity.
Leucine-rich PPR motif-containing protein, mitochondrial
FXYD domain-containing ion transport regulator
FXYD domain-containing ion transport regulator 4
Detected in mice with the buff mutation; leads to melanocytes with a reduced number of undermelanized melanosomes.
Vacuolar protein sorting-associated protein 33A
Putative sodium-coupled neutral amino acid transporter 10
39S ribosomal protein L13, mitochondrial
Cell surface glycoprotein CD200 receptor 1
Zinc finger and BTB domain-containing protein 8B
N-alpha-acetyltransferase 25, NatB auxiliary subunit
Potassium-transporting ATPase alpha chain 1
Coiled-coil-helix-coiled-coil-helix domain-containing protein 5
Abolishes interaction with CD4 endocytosis signal motif.
Reduces interaction with CD4 endocytosis signal motif.
Reduces interaction with CD4 endocytosis signal motif; when associated with AP2A2 E-21.
Slightly reduces interaction with CD4 endocytosis signal motif.
Cysteine-rich secretory protein LCCL domain-containing 1
Myosin light chain kinase 2, skeletal/cardiac muscle
Cytoplasmic dynein 1 light intermediate chain 2
U3 small nucleolar ribonucleoprotein protein IMP3
Does not affect core iron sulfur assembly complex formation. Abolishes sulhydratation.
Iron-sulfur cluster assembly enzyme ISCU
Nonsense-mediated mRNA decay factor SMG7
Calcium and integrin-binding family member 2
Causes deafness; decreases interaction with TMC1 and TMC2; abolishes localization of TMC1 to stereocilia; reduces localization of TMC2 to stereocilia; reduces calcium permeability and conductance of MET channels in hair cells.
Causes deafness; decreases interaction with TMC1 and TMC2; reduces MET currents.
Causes deafness; disrupts interaction with TMC1 and TMC2; abolishes MET currents; abolishes localization of TMC1 and TMC2 to stereocilia.
Causes profound deafness. Does not disrupt localization in hair cell stereocilia. No obvious indications of vestibular dysfunction.
Disrupts interaction with TMC1. Disrupts mechanoelectrical transduction (MET) in hair cells.
Abolishes IFNB1 production upon infection with various viruses.
Mitochondrial inner membrane protease subunit 2
Decreases interaction of PAK3 with CDC42 but increases interaction with RAC1.
When expressed in hippocampal neurons, decreases the number of dendritic spines. Strong decrease in the number of dendritic spines; when associated with L-78 and L-81.
When expressed in hippocampal neurons, increases the density of both spines and dendritic protrusions.
When expressed in hippocampal neurons, strongly decreases the number of dendritic spines; when associated with L-78 and R-312.
When expressed in hippocampal neurons, strongly decreases the number of dendritic spines; when associated with L-81 and R-312.
Formyl peptide receptor-related sequence 1
Glucose-fructose oxidoreductase domain-containing protein 1
Interchain (with C-577 in SUN2); alternate
Prolactin regulatory element-binding protein
Cell division cycle-associated protein 7
Myelin transcription factor 1-like protein
Vesicular inhibitory amino acid transporter
Activator of 90 kDa heat shock protein ATPase homolog 2
Coiled-coil domain-containing protein 85A
Steroid 17-alpha-hydroxylase/17,20 lyase
Ubiquitin carboxyl-terminal hydrolase 47
Breast carcinoma-amplified sequence 1 homolog
70% reduction of GAP activity; when associated with A-56.
70% reduction of GAP activity; when associated with V-169.
BTB/POZ domain-containing protein KCTD15
Abolishes autoinhibition, leading to increased kinase activity and constitutive phosphorylation of LYN substrates.
Phosphotyrosine; by autocatalysis, CSK and MATK
Sodium-coupled neutral amino acid transporter 9
Cyclic AMP-dependent transcription factor ATF-1
Phosphoserine; by CaMK1, CDK3, RPS6KA4 and RPS6KA5
39S ribosomal protein L38, mitochondrial
Osteopetrosis-associated transmembrane protein 1
CAP-Gly domain-containing linker protein 3
Transcriptional activator protein Pur-beta
Uracil nucleotide/cysteinyl leukotriene receptor
WD repeat and FYVE domain-containing protein 2
Interchain (with C-194 in C8-alpha chain)
Abolishes production of IL1B in response to bacterial flagellin.
Baculoviral IAP repeat-containing protein 1e
Mildly reduced interaction with flagellin.
Nearly abolishes interaction with flagellin.
No effect on production of IL1B in response to bacterial flagellin.
Strongly reduced interaction with flagellin.
Strongly reduces production of IL1B in response to bacterial flagellin.
Strongly reduces production of IL1B in response to bacterial flagellin; then associated with F-15.
Strongly reduces production of IL1B in response to bacterial flagellin; then associated with I-11.
Bactericidal permeability-increasing protein
SH3-containing GRB2-like protein 3-interacting protein 1
Leucine-rich repeat-containing protein 39
Receptor-type tyrosine-protein phosphatase H
Reduces tyrosine phosphorylation. Abolishes tyrosine phosphorylation and interaction with GRB2 and FYN; when associated with A-945.
Reduces tyrosine phosphorylation. Abolishes tyrosine phosphorylation and interaction with GRB2 and FYN; when associated with A-953.
von Hippel-Lindau disease tumor suppressor
PC-esterase domain-containing protein 1B
Abolishes FGF2-induced lens fiber differentiation via inhibition of FGF-mediated ERK1/2 phosphorylation.
Serine/threonine-protein kinase tousled-like 2
39S ribosomal protein L35, mitochondrial
Ubiquinol-cytochrome-c reductase complex assembly factor 3
Abolishes LPS-stimulated IL12B synthesis.
Microtubule-associated serine/threonine-protein kinase 2
DNA-dependent protein kinase catalytic subunit
Lethal at 4-week-old; impaired hematopoiesis due to a decrease in hematopoietic stem and progenitor cells which causes a severe reduction in the numbers of erythrocytes, platelets, lymphocytes and neutrophils; reduced protein synthesis in bone marrow and fetal erythrocyte precursors; normal V(D)J recombination in B-cells; when associated with 2614-A--A-2616, A-2634 and A-2643.
Lethal at 4-week-old; impaired hematopoiesis due to a decrease in hematopoietic stem and progenitor cells which causes a severe reduction in the numbers of erythrocytes, platelets, lymphocytes and neutrophils; reduced protein synthesis in bone marrow and fetal erythrocyte precursors; normal V(D)J recombination in B-cells; when associated with A-2605, 2614-A--A-2616 and A-2634.
Lethal at 4-week-old; impaired hematopoiesis due to a decrease in hematopoietic stem and progenitor cells which causes a severe reduction in the numbers of erythrocytes, platelets, lymphocytes and neutrophils; reduced protein synthesis in bone marrow and fetal erythrocyte precursors; normal V(D)J recombination in B-cells; when associated with A-2605, 2614-A--A-2616 and A-2643.
Lethal at 4-week-old; impaired hematopoiesis due to a decrease in hematopoietic stem and progenitor cells which causes a severe reduction in the numbers of erythrocytes, platelets, lymphocytes and neutrophils; reduced protein synthesis in bone marrow and fetal erythrocyte precursors; normal V(D)J recombination in B-cells; when associated with A-2605, A-2634 and A-2643.
Normal erythrocyte and platelet numbers; when associated with A-2026 and 2050-A--A-2053.
Normal erythrocyte and platelet numbers; when associated with A-2026 and A-2038.
Normal erythrocyte and platelet numbers; when associated with A-2038 and 2050-A--A-2053.
Probable loss of catalytic activity. Severe reduction in the number of hematopoietic stem and progenitor cells in fetal liver. Slight reduction in translation during erythrocyte development in fetal liver.
Mitochondrial coenzyme A diphosphatase NUDT8
Transcription initiation factor TFIID subunit 3
Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
In unc mutant; uncouples receptors from adenylyl cyclases.
Impairs calcium-dependent biosynthesis of NAPEs and NAEs. Reduces tubule growth and cargo transport from clathrin-independent endosomes.
Retinoic acid receptor responder protein 2
Peptidyl-prolyl cis-trans isomerase FKBP5
Ankyrin repeat and BTB/POZ domain-containing protein 3
28S rRNA (cytosine-C(5))-methyltransferase
Thyroid hormone-inducible hepatic protein
Putative ubiquitin carboxyl-terminal hydrolase 50
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Intraflagellar transport protein 27 homolog
EF-hand domain-containing family member B
Janus kinase and microtubule-interacting protein 3
Coiled-coil domain-containing protein 159
RNA-binding motif protein, Y chromosome, family 1 member A1
Causes a failure of Shh processing leading to retention of the immature glycosylated protein within the endoplasmic reticulum of transfected cells; causes a temperature-dependent conformational change that allows Shh to bind Ptch1 at 4 or 32 degrees Celsius but not at 37 degrees Celsius; drastically reduces signaling potency in chicken embryo neural plate explant assays.
Does not affect signaling activity in any of Shh signaling assays and causes no apparent defects in cholesterol-mediated autoprocessing reactions.
Introduces a cleavage site for a furin-like protease resulting in abnormal protein processing; cleavage at this site removes 11 amino acids from the N-terminal domain and reduces affinity of Shh for Ptch1 and signaling potency in assays using chicken embryo neural plate explants and mouse C3H10T1/2 stem cells.
Moderately reduces Ptch1 binding in vitro and signaling potency in chicken embryo neural plate explant assays compared with wild-type sequence.
Shows no change in activities at different temperatures.
Strongly reduces effects of in vivo overexpression; impairs multimer formation; does not affect subcellular location to lipid rafts. Homozygous mice are characterized by a smaller size and holoprosencephaly at 10.5 dpc, and shortening of limbs at 13.5 dpc. They die soon after birth.
DNA damage-regulated autophagy modulator protein 2
Centriole and centriolar satellite protein OFD1
Induces centriole elongation. Impaired CEP164 and IFT88 recruitment. Impaired ciliogenesis.
Induces centriole elongation. Impaired IFT88 recruitment. Impaired ciliogenesis.
Induces centriole elongation. Impaired IFT88 recruitment. Reduced ciliogenesis.
Induces centriole elongation. Reduced ciliogenesis.
Shortened centrioles. Reduced ciliogenesis.
Xylosyl- and glucuronyltransferase LARGE2
In Past-time (Psttm) mutant; causes period shortening due to Cry1 and Cry2 destabilization.
Solute carrier organic anion transporter family member 1A5
Cilia- and flagella-associated protein 20
Microtubule-associated serine/threonine-protein kinase 4
Zinc finger and SCAN domain-containing protein 10
Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C.
Abrogates ubiquitination activity and the ability to promote DR4- and Fas-induced apoptosis.
Low-density lipoprotein receptor-related protein 1 515 kDa subunit
Low-density lipoprotein receptor-related protein 1 85 kDa subunit
Low-density lipoprotein receptor-related protein 1 intracellular domain
Prolow-density lipoprotein receptor-related protein 1
Recognition site for proteolytical processing
Mice carrying this mutation show atrophic ovaries without oocytes, but no other visible phenotype.
Strongly reduced interaction with EIF4A1.
Late cornified envelope-like proline-rich protein 1
N6-(pyridoxal phosphate)lysine; alternate
Serine hydroxymethyltransferase, mitochondrial
Chorion-specific transcription factor GCMa
Glycerol-3-phosphate dehydrogenase 1-like protein
Abolished acyltransferase activity. Impaired ability to interact with WNT3A.
Does not affect acyltransferase activity.
Impaired acyltransferase activity. Impaired ability to interact with WNT3A.
Protein-serine O-palmitoleoyltransferase porcupine
Endothelial cell-specific chemotaxis regulator
Zinc finger CCCH-type antiviral protein 1
ADP-ribosylation factor GTPase-activating protein 1
Leucine-rich repeat-containing protein 34
In isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6.
Soluble interleukin-15 receptor subunit alpha
Solute carrier organic anion transporter family member 2A1
Putative sodium-coupled neutral amino acid transporter 8
Eukaryotic translation initiation factor 2 subunit 2
Centromere/kinetochore protein zw10 homolog
Multiple inositol polyphosphate phosphatase 1
Eukaryotic translation initiation factor 3 subunit M
Marginal zone B- and B1-cell-specific protein
Reduced electrophoretic mobility; when associated with A-170.
Reduced electrophoretic mobility; when associated with A-177.
Reduced electrophoretic mobility; when associated with A-49.
Reduced electrophoretic mobility; when associated with A-52.
Forkhead-associated domain-containing protein 1
Serine/threonine-protein phosphatase with EF-hands 2
CKLF-like MARVEL transmembrane domain-containing protein 2A
EGF-like and EMI domain-containing protein 1
ATP synthase-coupling factor 6, mitochondrial
NADPH-dependent diflavin oxidoreductase 1
Cell division cycle-associated 7-like protein
Beta-1,4 N-acetylgalactosaminyltransferase 2
Electron transfer flavoprotein subunit beta
N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
DNA replication complex GINS protein PSF2
Adaptin ear-binding coat-associated protein 1
Loss of binding to AP-2 and can bind to AP-1; when associated with D-270.
Loss of binding to AP-2 and can bind to AP-1; when associated with G-273.
Chromodomain-helicase-DNA-binding protein 2
Soluble secretory phospholipase A2 receptor
Abolishes the ability to mediate myoblast fusion; does not affect interaction with MYMK; does not affect localization to the cell membrane.
Decreased interaction with MYMK without affecting localization to the cell membrane.
Calmodulin-binding transcription activator 2
Glutathione S-transferase A1, N-terminally processed
N-acetylalanine; in Glutathione S-transferase A1, N-terminally processed
Potassium voltage-gated channel subfamily H member 7
Leucine-rich repeat-containing protein 41
No association with Elongin BC complex; when associated with F-50.
No association with Elongin BC complex; when associated with P-46.
Dimethylglycine dehydrogenase, mitochondrial
Sterile alpha motif domain-containing protein 13
Bifunctional glutamate/proline--tRNA ligase
Constitutively active in translation inhibition (phosphomimetic). Mutant mice have no apparent developmental defect and do not display overt phenotype related to adiposity or lifespan.
Loss of function in translation inhibition. Loss of interaction with SLC27A1. Mutant mice have no apparent developmental defect but display reduced adiposity associated with decreased insulin levels and adipocytes size. They also display increased lipolysis and fatty acid beta-oxidation and an extended lifespan. Adipocytes display decreased insulin-stimulated long-chain fatty acid uptake.
Pogo transposable element with ZNF domain
POU domain, class 5, transcription factor 2
Strain BALB/c. Without methionine sulfoxide.
Strain C57BL/6. Without methionine sulfoxide.
Lutropin-choriogonadotropic hormone receptor
Homozygous mice have profound B cell immunodeficiency and impaired transition from late pre-B to immature B cell. B cells from mutant mice exhibit a diminished concentration of cytoplasmic free zinc and decreased phosphorylation of signaling molecules downstream of the pre-B cell and B cell receptors.
39S ribosomal protein L53, mitochondrial
Structure-specific endonuclease subunit SLX1
Dyslexia-associated protein KIAA0319 homolog
A disintegrin and metalloproteinase with thrombospondin motifs 2
Abolishes phosphorylation by PKA. No change in protein levels. Loss of protein stability on PKA stimulation mediated repression. No change in interaction with HDAC4 in response to PKA; when associated with A-121.
Abolishes phosphorylation by PKA. No change in protein levels. Loss of protein stability on PKA stimulation. Loss of PKA-mediated repression. No change in interaction with HDAC4 in response to PKA; when associated with A-190.
Loss of calpain/Cdk5-mediated neuron apoptosis.
PHD finger-like domain-containing protein 5A
Glycine cleavage system H protein, mitochondrial
Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
Loss of p53/TP53-deubiquitinating activity.
Mitogen-activated protein kinase kinase kinase 14
Does not reduce affinity fo GTP and GDP. Enhances interaction with PDE6D.
Reduces affinity to GTP and GDP. Inhibits interaction with PDE6D.
Ovarian cancer G-protein coupled receptor 1
O-(5'-phospho-DNA)-tyrosine intermediate
ADP-ribosylation factor-like protein 13A
Solute carrier family 2, facilitated glucose transporter member 10
Sulfide:quinone oxidoreductase, mitochondrial
Long-chain-fatty-acid--CoA ligase ACSBG2
Transmembrane emp24 domain-containing protein 11
Activated RNA polymerase II transcriptional coactivator p15
Partially reduces its import in the nucleus.
Phosphorylated adapter RNA export protein
Reduces U snRNA export. Strongly abolishes the U snRNA export complex formation. Does not abolish the pre-complex formation.
Strongly reduces its import in the nucleus. Abolishes its import in the nucleus, does not change its export to the cytoplasm and the export of U snRNA complex; when associated with 190-AA-191.
Strongly reduces its import in the nucleus. Abolishes its import in the nucleus, does not change its export to the cytoplasm and the export of the U snRNA complex; when associated with 71-ARAA-74.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO; alternate)
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin; alternate)
Ectonucleoside triphosphate diphosphohydrolase 2
Motile sperm domain-containing protein 2
Probable E3 ubiquitin-protein ligase HERC4
Progressive ataxia associated with Purkinje cell death. Retinal thinning due to photoreceptor cell death.
Trafficking protein particle complex subunit 9
Fibronectin type III domain-containing protein 11
Proprotein convertase subtilisin/kexin type 9
Ankyrin repeat and SAM domain-containing protein 4B
PDZ-binding; mediates interaction with USH1C
Chloride intracellular channel protein 4
N-acetylalanine; in cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed
cAMP-dependent protein kinase type I-alpha regulatory subunit
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed
Methyl-CpG-binding domain protein 3-like 1
Abolished 2'-5'-oligoadenylate synthase activity.
Mice display postpartum failure of lactation and strongly reduced milk protein synthesis. Otherwise, mice develop normally and mammary glands are normal. The mutation causes an activation of the OAS2 pathway, characterized by a strong increase of Ribonuclease L (RNASEL) activity.
Strongly reduced 2'-5'-oligoadenylate synthase activity.
Zinc finger HIT domain-containing protein 1
Putative E3 ubiquitin-protein ligase UBR7
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Musculoskeletal embryonic nuclear protein 1
Epidermal growth factor receptor kinase substrate 8-like protein 2
Nuclear receptor subfamily 1 group D member 2
N-acetylthreonine; in N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processed
N-terminal Xaa-Pro-Lys N-methyltransferase 1
N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processed
Acyl-coenzyme A synthetase ACSM5, mitochondrial
Loss of interferon-gamma induced phosphorylation.
Loss of kinase activity; no effect on FIZ1-binding.
Receptor-type tyrosine-protein kinase FLT3
2-oxoadipate dehydrogenase complex component E1
Corresponds to C-356 variant associated with SPGF16 in human. Impairs nuclear inner membrane location and decreases interaction with DNAJB13.
Corresponds to I-348 variant associated with SPGF16 in human. Impairs nuclear inner membrane location and increases interaction with DNAJB13.
Corresponds to M-261 variant associated with SPGF16 in human. Decreases protein solubility, impairs nuclear inner membrane location and decreases interaction with DNAJB13.
Corresponds to M-275 variant associated with SPGF16 in human. Impairs nuclear inner membrane location and decreases interaction with DNAJB13.
Corresponds to R-114 variant associated with SPGF16 in human. Impairs nuclear inner membrane location, expressed in the cytosol with some protein aggregation near the nuclear envelope.
Corresponds to variant K-162 associated with SPGF16 in human. Increases interaction with DNAJB13. Impairs nuclear inner membrane location, expressed in the cytosol with some protein aggregation near the nuclear envelope.
Coiled-coil domain-containing protein 12
Abolishes dimethylation. Impairs transcription coactivator activity and regulation of alternative splicing. No effect on methyltransferase activity.
Abolishes histone methyltransferase activity and coactivator activity.
Abolishes histone methyltransferase activity and reduces coactivator activity.
Histone-arginine methyltransferase CARM1
Loss of S-adenosyl-L-methionine binding. Loss of protein methyltransferase activity.
Loss of S-adenosyl-L-methionine binding. Loss of protein methyltransferase activity. Localized in the cytosol.
Loss of S-adenosyl-L-methionine binding. Loss of protein methyltransferase activity. Localized in the nucleus.
Loss of protein methyltransferase activity.
Reduces protein methyltransferase activity.
ADP-ribosylcysteine; by autocatalysis; in irreversibly inhibited form
Abolishes sulfhydration and induces impaired enzyme activity.
Glyceraldehyde-3-phosphate dehydrogenase
S-nitrosocysteine; in reversibly inhibited form
Loss of PAR-binding and of protection against DNA damage; when associated with A-156.
Loss of PAR-binding and of protection against DNA damage; when associated with A-157.
Loss of ubiquitination activity. Loss of protection against DNA damage. No effect on PAR-binding.
Small glutamine-rich tetratricopeptide repeat-containing protein beta
Abolishes localization to microtubules minus ends; decreases ASPM localization to microtubules minus ends; no enhancement of ASPM activity in blocking microtubule minus-end growth.
Disrupts KATNA1:KATNB1 interaction with ASPM.
Disrupts KATNA1:KATNB1 interaction with ASPM; abolishes localization to microtubules minus ends; decreases ASPM localization to microtubules minus ends; no enhancement of ASPM activity in blocking microtubule minus-end growth.
Katanin p80 WD40 repeat-containing subunit B1
Loss of cMYC-induced CDK2-mediated phosphorylation Dissociates very slowly from the cyclin E/CDK2 complex after induction by cMYC. Cell cycle arrest.
Loss of cMyc-induced CDK2-mediated phosphorylation. Rapid dissociation from the cyclin E/CDK2 complex after induction by c-Myc.
Loss of interaction with NUP50. No cyclin E-mediated degradation of phosphorylated p27KIP1.
Loss of phosphorylation in G(0) phase. No change in cMYC-induced CDK2-mediated phosphorylation. Rapid dissociation from the cyclin E/CDK2 complex after induction by cMYC. Loss of protein stability in G(0) phase. No change in protein stability at S-phase.
Phosphothreonine; by CaMK1, PKB/AKT1, RPS6KA1, RPS6KA3 and PIM1
Phosphothreonine; by PKB/AKT1, CDK1 and CDK2
Phosphotyrosine; by ABL, LYN, SRC and JAK2
Coiled-coil domain-containing protein 167
Abolished RAC1 GTPase activity. Abolished ability to induce neurite branching. No effect on filopodia biogenesis and neurite outgrowth.
Loss of the ability to induce filopodia and to initiate neurite outgrowht. Abolished interaction with GPHN.
SLIT-ROBO Rho GTPase-activating protein 2
26S proteasome non-ATPase regulatory subunit 1
Tumor necrosis factor receptor superfamily member 3
Cytoplasmic polyadenylation element-binding protein 4
10-fold loss in affinity to sialic acid.
RNA polymerase II elongation factor ELL2
Abolishes insulin-induced phosphorylation when associated with A-463. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-316; A-462 and A-463.
Decreased transcriptional activity by about 2-fold in the absence of serum; when associated with A-463. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-253; A-316 and A-462.
Decreased transcriptional activity by about 2-fold in the absence of serum; when associated with A-463. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-253; A-316 and A-463.
Decreases insulin-induced phosphorylation by approximately 30%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-24; A-253; A-462 and A-463.
Decreases insulin-induced phosphorylation by approximately 30%. Nuclear location but transcriptional activity decreased by about 50%. Abolishes the SIRT1 deacetylase binding and increases acetylation; when associated with A-253; A-316; A-462 and A-463. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with Q-219; Q-242; Q-245; Q-259; Q-262; Q-271 and Q-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with R-219; R-242; R-245; R-259; R-262; R-271 and R-291.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-326; A-380; A-391; A-399 and A-413.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-326; A-380; A-391; A-399 and A-415.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-326; A-380; A-391; A-413 and A-415.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-326; A-380; A-399; A-413 and A-415.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-326; A-391; A-399; A-413 and A-415.
Decreases phosphorylation by NLK; when associated with A-284; A-295; A-380; A-391; A-399; A-413 and A-415.
Decreases phosphorylation by NLK; when associated with A-284; A-326; A-380; A-391; A-399; A-413 and A-415.
Decreases phosphorylation by NLK; when associated with A-295; A-326; A-380; A-391; A-399; A-413 and A-415.
Little change in levels of methylation; when associated with K-147; K-154; K-311 and K-313.
Little change in levels of methylation; when associated with K-29; K-147; K-154 and K-311.
Little change in levels of methylation; when associated with K-29; K-147; K-154 and K-313.
Little change in levels of methylation; when associated with K-29; K-147; K-311 and K-313.
Little change in levels of methylation; when associated with K-29; K-154; K-311 and K-313.
Mimics acetylation. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-242; Q-245; Q-259; Q-262 and Q-271. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24: Q-219; Q-242; Q-245; Q-259; Q-262 and Q-271.
Mimics acetylation. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-242; Q-245; Q-259; Q-262 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-219; Q-242; Q-245; Q-259; Q-262 and Q-291.
Mimics acetylation. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-242; Q-245; Q-262; Q-271 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-219; Q-242; Q-245; Q-262; Q-271 and Q-291.
Mimics acetylation. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-242; Q-245; Q-259; Q-262; Q-271 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-242; Q-245; Q-259; Q-262; Q-271 and Q-291.
Mimics acetylation. Cytoplasmic location in absence or presence of insulin. Decreased DNA-binding by about half. Enhanced phosphorylation by PKB/AKT1, no effect on interaction with CEBPA; when associated with either A-245 or Q-245 and either A-262 or Q-262. either A-262 or Q-262. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-245; Q-259; Q-262; Q-271 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-219; Q-245; Q-259; Q-262; Q-271 and Q-291.
Mimics acetylation. Decreased DNA-binding by about half and enhanced phosphorylation by PKB/AKT1, no effect on interaction with CEBPA; when associated with either A-242 or Q-242 and either A-245 or Q-245. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-242; Q-245; Q-259; Q-271 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-219; Q-242; Q-245; Q-259; Q-271 and Q-291.
Mimics acetylation. Decreased DNA-binding by about half. Enhanced phosphorylation by PKB/AKT1, no effect on interaction with CEBPA; when associated with either A-242 or Q-242 and either A-262 or Q-262. Cytoplasmic location in absence or presence of insulin, no change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with Q-219; Q-242; Q-259; Q-262; Q-271 and Q-291. Increased insulin-induced phosphorylation at Ser-253 and binding of 14-3-3 proteins; when associated with A-24; Q-219; Q-242; Q-259; Q-262; Q-271 and Q-291.
Phosphothreonine; by PKB/AKT1 or PKB/AKT2 and SGK1
Reduced acetylation and transcriptional activity increased by about 1.5 fold. Completely abolishes acetylation, increases interaction with CEBPA and transcriptional activity increased by about 3-fold; when associated with R-245 and R-262. Transcriptional activity not inhibited by FCOR; when associated with R-245; R-259; R-262; R-271 and R-291. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-245; R-259; R-262; R-271 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-245; R-259; R-262; R-271 and R-291.
Reduced acetylation and transcriptional activity increased by about 1.5-fold. Completely abolishes acetylation, increases interaction with CEBPA and transcriptional activity increased by about 3-fold; when associated with R-242 and R-262. Transcriptional activity not inhibited by FCOR; when associated with R-242; R-259; R-262; R-271 and R-291. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-242; R-259; R-262; R-271 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-242; R-259; R-262; R-271 and R-291.
Significant reduction in acetylation and transcriptional activity increased by about 2.0 fold. Completely abolishes acetylation, increases interaction with CEBPA and transcriptional activity increased by about 3-fold; when associated with R-242 and R-245. Transcriptional activity not inhibited by FCOR; when associated with R-242; R-245; R-259; R-271 and R-291. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-242; R-245; R-259; R-271 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-242; R-245; R-259; R-271 and R-291.
Some decrease in levels of methylation. Greatly decreased methylation levels; when associated with K-248.
Some decrease in levels of methylation. Greatly decreased methylation levels; when associated with K-250.
Transcriptional activity not inhibited by FCOR; when associated with R-242; R-245; R-259; R-262 and R-271. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-242; R-245; R-259; R-262 and R-271. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-242; R-245; R-259; R-262 and R-271.
Transcriptional activity not inhibited by FCOR; when associated with R-242; R-245; R-259; R-262 and R-291. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-242; R-245; R-259; R-262 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-242; R-245; R-259; R-262 and R-291.
Transcriptional activity not inhibited by FCOR; when associated with R-242; R-245; R-262; R-271 and R-291. Predominantly nuclear and translocates to the cytoplasm on insulin-stimulation. No change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-219; R-242; R-245; R-262; R-271 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-219; R-242; R-245; R-262; R-271 and R-291.
Translocates to the cytoplasm after insulin-stimulation. No change on the inhibitory effect of oxidative stress on insulin-induced phosphorylations, but no inhibition of these phosphorylations by resveratrol; when associated with R-242; R-245; R-259; R-262; R-271 and R-291. Increased binding of 14-3-3 proteins even with decreased insulin-induced phosphorylation at Ser-253; when associated with A-24; R-242; R-245; R-259; R-262; R-271 and R-291.
Mas-related G-protein coupled receptor member B3
WAP four-disulfide core domain protein 8
Jupiter microtubule associated homolog 1
Jupiter microtubule associated homolog 1, N-terminally processed
N-acetylthreonine; in Hematological and neurological expressed 1 protein, N-terminally processed
Voltage-dependent L-type calcium channel subunit alpha-1D
Suppressor of tumorigenicity 14 protein homolog
Long-chain fatty acid transport protein 6
Interactor protein for cytohesin exchange factors 1
Dynein light chain Tctex-type protein 2B
Endoplasmic reticulum transmembrane helix translocase
DALR anticodon-binding domain-containing protein 3
Sodium/potassium-transporting ATPase subunit alpha-1
Abolishes BMP1-mediated cleavage of ectodomain release of olfactomedin-like domain.
Abolishes furin-mediated cleavage and shedding of ectodomain.
In strain: 020, BALB/c, BALB/cJ, C3H/21BG, C3H/HeJ, CBA/J, MA/M4J and PL/J.
In strain: BALB/cJ, C57BL/6J and MOLF/Ei.
Hypomorphic allele. Abrogates interaction with EZH2 and impairs transcriptional repression.
Null allele. Abrogates interaction with EZH2 and impairs transcriptional repression.
Androgen-dependent TFPI-regulating protein
IQ and ubiquitin-like domain-containing protein
Abolished homodimerization and subsequent activation.
Reduced homodimerization and subsequent activation.
Glucose-dependent insulinotropic receptor
Psoriasis susceptibility 1 candidate gene 2 protein homolog
Decrease in ACHE tetramer recruitment to membrane rafts.
Does not abolish the localization of ACHE.
No effect on ACHE tetramer recruitment to membrane rafts.
No effect on ACHE tetramer recruitment to membrane rafts; when associated with 138-A--A-142.
No effect on ACHE tetramer recruitment to membrane rafts; when associated with A-131.
Ras association domain-containing protein 1
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
Zinc finger CCHC domain-containing protein 18
Cytochrome c oxidase subunit 5A, mitochondrial
Transcription elongation factor A protein 2
Coiled-coil domain-containing protein 103
Abolished cystine transport. Abolished interaction with components of the v-ATPase and Ragulator complexes. Does not affect ability to lysosomal localization of LAMP2A.
Cystinosin, lysosomal cystine transporter
protonated residue following cystine-binding
Ubiquitin carboxyl-terminal hydrolase isozyme L4
Enhances basal channel activity and abolishes stimulation by calcineurin.
Loss of interaction with calcineurin and activation by elevated intracellular calcium; when associated with A-214.
Strongly reduced activation by elevated intracellular calcium. Loss of interaction with calcineurin and activation by elevated intracellular calcium; when associated with A-212.
Strongly reduced current amplitude and localization to cell membrane.
Abolishes phosphatidate phosphatase activity but does not prevent membrane association nor coactivator activity.
Abolishes phosphatidate phosphatase activity but does not prevent membrane association.
Regulator of microtubule dynamics protein 2
Loss of cleavage by asparagine endopeptidase (AEP).
No effect on cleavage by asparagine endopeptidase (AEP).
ATP-sensitive inward rectifier potassium channel 12
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3
Abolishes conductivity; when associated with A-349 and A-350.
Abolishes conductivity; when associated with A-349 and A-351.
Abolishes conductivity; when associated with A-350 and A-351.
Abolishes sensitivity to sulfhydryl modification.
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Reduces affinity for cAMP and impairs tetramerization.
MAPK-interacting and spindle-stabilizing protein-like
Abolishes lowering of temperature threshold for activation in response to reactive oxygen species.
Transient receptor potential cation channel subfamily M member 2
Oxysterol-binding protein-related protein 3
TRAF-interacting protein with FHA domain-containing protein A
MAP kinase-interacting serine/threonine-protein kinase 2
Normal MAPK3/ERK1 and MAPK1/ER2K-binding.
Normal MAPK3/ERK1 and MAPK1/ERK2-binding.
Reduced MAPK3/ERK1 and MAPK1/ER2K-binding.
Reduced MAPK3/ERK1 and MAPK1/ERK2-binding.
tRNA-splicing endonuclease subunit Sen54
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8
Ubiquitin-fold modifier-conjugating enzyme 1
T-cell-specific guanine nucleotide triphosphate-binding protein 1
T-cell-specific guanine nucleotide triphosphate-binding protein 2
No effect on interaction with BTRC and FBXW11.
Abolishes binding of Mg(2+) and increases pore diameter. Impairs intestinal Ca(2+) uptake.
Abolishes plasma membrane localization and channel activity.
Transient receptor potential cation channel subfamily V member 6
ELKS/Rab6-interacting/CAST family member 1
Loss of phosphorylation and interaction with SRC, and inhibition of bone resorption.
Decreased calcium-dependent cell-cell adhesion.
Loss of calcium-dependent cell-cell adhesion.
Loss of dimerization; when associated with A-161.
Loss of dimerization; when associated with E-173.
Severely impaired the binding of calcium to all three sites.
RUN and FYVE domain-containing protein 2
Leucine-rich repeat and death domain-containing protein 1
Abolishes ligand binding and reduces transactivation; when associated with W-270.
In strain: C3H/HeJ and DBA/2J; does not change transcriptional activity.
Reduced lipid binding and transactivation. Abolishes ligand binding and reduces transactivation; when associated with F-345.
Strongly reduced transactivation; when associated with D-440.
Strongly reduced transactivation; when associated with D-442.
3-hydroxy-3-methylglutaryl-coenzyme A reductase
G-protein coupled receptor-associated sorting protein 2
Large neutral amino acids transporter small subunit 4
Abolishes myristoylation and palmitoylation.
Abolishes palmitoylation and plasma membrane association; when associated with A-3.
Abolishes palmitoylation and plasma membrane association; when associated with A-6.
Abolishes palmitoylation and plasma membrane association; when associated with S-3.
Abolishes palmitoylation and plasma membrane association; when associated with S-6. Abolishes plasma membrane association.
Homozygous mutant mice exhibit retinal degeneration.
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
Abolishes the interactions with estrogen and retinoid-acids receptors.
Multiple epidermal growth factor-like domains protein 8
Loss of protein-cysteine S-palmitoyltransferase activity.
Decreases plasma membrane aminophospholipid translocation in neuronal cells.
Mitochondrial import inner membrane translocase subunit Tim17-B
Coiled-coil domain-containing protein 73
Acyl-coenzyme A synthetase ACSM2, mitochondrial
Hyaluronan and proteoglycan link protein 4
Inactive glycosyltransferase 25 family member 3
Constitutively active. Interacts with GARIN1B.
Constitutively inactive. Loss of interaction with GARIN1B.
Transmembrane and immunoglobulin domain-containing protein 1
Cilia- and flagella-associated protein 45
39S ribosomal protein L30, mitochondrial
Complete loss of STAT1 and STAT3 activation.
Complete loss of interaction with SOCS1.
Abolished lysine demethylase activity; when associated with A-302.
Abolished lysine demethylase activity; when associated with A-367.
Decreases myeloid cell immune response. Reduces expression levels.
Macrophages exhibit increased apoptosis, reduced proliferation, and reduced phagocytosis. Macrophages also exhibit increased inflammatory cytokine secretion. Microglia demonstrate reduced immune activity. Also decreases cerebral blood flow and reduces cerebral glucose metabolism.
Triggering receptor expressed on myeloid cells 2
Mothers against decapentaplegic homolog 1
Phosphothreonine; by MINK1, TNIK and MAP4K4
Mutant male mice exhibit meiotic arrest, azoospermia and infertility. Females exhibit infertility or low reproductive capacity.
Cysteine-rich hydrophobic domain-containing protein 2
Double-stranded RNA-binding protein Staufen homolog 1
Staufen double-stranded RNA binding protein 1
Interleukin-3 receptor class 2 subunit beta
Gamma-aminobutyric acid receptor-associated protein
Abolishes phosphorylation by PKC and impairs Golgi targeting; no effect on phospholipid transfer activity.
No effect on phosphorylation by PKC; abolishes phospholipid transfer activity.
Phosphatidylinositol transfer protein beta isoform
Histone-lysine N-trimethyltransferase SMYD5
Cytoplasmic 60S subunit biogenesis factor ZNF622
Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.
In ST; causes an absence of mature T-cells due to thymocyte development being arrested at the CD4+CD8+ stage.
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Capping protein, Arp2/3 and myosin-I linker protein 3
High affinity immunoglobulin epsilon receptor subunit beta
Vesicle-associated membrane protein-associated protein A
Chloride intracellular channel protein 1
Endoplasmic reticulum metallopeptidase 1
Disintegrin and metalloproteinase domain-containing protein 1a
2-(3-amino-3-carboxypropyl)histidine synthase subunit 1
Abolishes plasma membrane association; when associated with S-21.
Abolishes plasma membrane association; when associated with S-41. Reduced palmitoylation level.
Complete loss of interaction with CD4 or CD8.
Proto-oncogene tyrosine-protein kinase LCK
POU domain, class 3, transcription factor 1
Myelin-associated oligodendrocyte basic protein
Abolishes disulfide-dependent homodimerization. Abolishes disulfide-dependent homodimerization; when associated with A-21.
Abolishes disulfide-dependent homodimerization; when associated with A-10. Does not affect disulfide-dependent homodimerization; when associated with A-33.
Does not affect disulfide-dependent homodimerization; when associated with A-21.
Does not affect ability to promote ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis).
High affinity nerve growth factor receptor
Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic
Iron-sulfur cluster co-chaperone protein HscB, mitochondrial
Synaptic vesicle membrane protein VAT-1 homolog
Vacuolar protein sorting-associated protein 72 homolog
Phosphate-regulating neutral endopeptidase PHEX
Mas-related G-protein coupled receptor member G
Phosphoinositide 3-kinase regulatory subunit 5
WAS/WASL-interacting protein family member 1
Gamma-aminobutyric acid receptor subunit rho-1
Loss of cardiac myofibril assembly; when associated with A-14 and A-15. Loss of phosphorylation; when associated with A-14 and A-15. Loss of calcium sensitivity of force development; when associated with A-14 and A-15.
Loss of cardiac myofibril assembly; when associated with A-14 and A-19. Loss of phosphorylation; when associated with A-14 and A-19. Loss of calcium sensitivity of force development; when associated with A-14 and A-19. Increase of phosphorylation. Phosphorylation at Ser-14 and at Ser-19. Phosphorylation at Ser-19; when associated with A-14. Significant decrease in phosphorylation by MLCK; when associated with A-14. Adult lethality associated with early defects in cardiac twitch relaxation and torsion leading to dilated cardiomyopathy, heart failure and premature death; when associated with A-14. Significant acceleration of twitch relaxation in absence of changes in calcium transients; when associated with A-14. Absence of transmural phosphorylation gradient leading to alteration of torsion; when associated with A-14.
Loss of cardiac myofibril assembly; when associated with A-15 and A-19. Loss of phosphorylation; when associated with A-15 and A-19. Loss of calcium sensitivity of force development; when associated with A-15 and A-19. Phosphorylation at Ser-19; when associated with A-15. Significant decrease in phosphorylation by MLCK; when associated with A-15. Adult lethality associated with early defects in cardiac twitch relaxation and torsion leading to dilated cardiomyopathy, heart failure and premature death; when associated with A-15.Significant acceleration of twitch relaxation in absence of changes in calcium transients; when associated with A-15. Absence of transmural phosphorylation gradient leading to alteration of torsion; when associated with A-14.
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Interchain (between alpha-2-1 and delta-1 chains)
Voltage-dependent calcium channel subunit alpha-2-1
Voltage-dependent calcium channel subunit alpha-2/delta-1
Voltage-dependent calcium channel subunit delta-1
Interchain (with C-608 or C-609 in GP1BA)
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Alters leukotriene hydrolase activity, strongly enhancing the formation of a metabolite that is normally produced in trace amounts.
Complete loss of LTA4 hydrolase and peptidase enzyme activities.
Asymmetric dimethylarginine; by PRMT1 and PRMT5
Set1/Ash2 histone methyltransferase complex subunit ASH2
Actin, alpha cardiac muscle 1, intermediate form
Dephospho-CoA kinase domain-containing protein
Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 49-A-A-50 and 120-A-A-121.
Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 49-A-A-50 and 79-A-A-80.
Abolishes production of the mature chain and promotes apoptosis of superior cervical ganglion neurons; when associated with 79-A-A-80 and 120-A-A-121.
Near loss of the ability to trigger histamine release from mast cells. No effect on interaction with NTRK1.
Steroidogenic acute regulatory protein, mitochondrial
Probable proton-coupled zinc antiporter SLC30A4
Mas-related G-protein coupled receptor member A1
Abolishes piRNA-binding and ubiquitination by the APC/C.
Does not affect piRNA-binding but abolishes ubiquitination by the APC/C. Causes male sterility.
Impairs binding to 2'-O-methylated 3'-end of piRNAs.
In DAH mutant; causes male infertility due to derepression of LINE1 retrotransposons transcripts.
Prostate and testis expressed protein 14
Impaired interaction with FBXL17, leading to decreased ubiquitination by the SCF(FBXL17) complex.
Increased interaction with FBXL17, leading to increased ubiquitination by the SCF(FBXL17) complex.
Increased stability of the protein caused by impaired ubiquitination by the SCF(FBXL17) complex.
Mimicks residue acetylation; impaired interaction with FBXL17, leading to decreased ubiquitination by the SCF(FBXL17) complex.
Mimicks residue acetylation; increased interaction with FBXL17, leading to increased ubiquitination by the SCF(FBXL17) complex.
Slightly reduced degradation, probably caused by reduced ubiquitination by the SCF(FBXL17) complex.
ABC-type organic anion transporter ABCA8A
Does not affect ubiquitination by TRIM38.
TIR domain-containing adapter molecule 1
Inactive GDP-bound form which does not bind TJAP1.
Loss of activity; delays formation of epithelial polarity.
Loss of interaction with KIF23, GGA1, SPAG9 and RAB11FIP4.
Probable constitutively active mutant that prevents EHD1 localization to endosome membranes. No effect on interaction with TJAP1.
Slightly impaired interaction with KIF23. Abolishes interaction with GGA1, SPAG9 and RAB11FIP4.
Absence of proteolytic activity. Loss of its processing into the mature form.
Complement C1s-B subcomponent heavy chain
Complement C1s-B subcomponent light chain
Collagen and calcium-binding EGF domain-containing protein 1
26S proteasome non-ATPase regulatory subunit 2
Sodium-dependent phosphate transport protein 3
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Tumor necrosis factor receptor superfamily member 14
Synaptic vesicle membrane protein VAT-1 homolog-like
In Mot; male sterility characterized by reduced sperm output, and sperm with aberrant motility and short tails.
Abolishes localization with replicating mitochondria.
Abolishes nuclear export and interactions with itself and p75NTR/NGFR; when associated with A-94 and A-97.
Abolishes nuclear export and interactions with itself and p75NTR/NGFR; when associated with A-97 and A-99.
Mediator of RNA polymerase II transcription subunit 25
Basic helix-loop-helix ARNT-like protein 1
Decreased acetylation, leading to decreased transcription elongation during the activation phase of the circadian cycle.
Decreases without abolishing O-GlcNAcylation.
Impaired nuclear accumulation, decreased interaction with CLOCK and disruption of circadian clock function.
Reduced CLOCK binding. Abolishes transcriptional activation by the CLOCK-BMAL1 heterodimer.
Reduced CLOCK binding. Slightly reduced transcriptional activation by the CLOCK-BMAL1 heterodimer. Impairs regulation of circadian clock. Loss of ability to inhibit the expression of CD274 in macrophages.
Significant decrease in; transcriptional activity, localization in PML body, ubiquitination and proteasome-mediated proteolysis.
Significant reduction in nucleocytoplasmic shuttling; when associated with A-154.
Significant reduction in nucleocytoplasmic shuttling; when associated with A-157.
Significant reduction in nucleocytoplasmic shuttling; when associated with A-370.
Significant reduction in nucleocytoplasmic shuttling; when associated with A-374.
Mediator of RNA polymerase II transcription subunit 28
WAP four-disulfide core domain protein 3
Abolishes binding to phosphorylated Lilrb4a.
Mice display a low bone mass density and are associated with osteopenia and elevated inflammatory cytokines.
Slight reduction in binding to phosphorylated Lilrb4a.
Tyrosine-protein phosphatase non-receptor type 6
Secreted Ly-6/uPAR domain-containing protein 2
3-phosphoinositide-dependent protein kinase 1
G protein-activated inward rectifier potassium channel 2
Transcription elongation factor A protein-like 8
C-Jun-amino-terminal kinase-interacting protein 3
Results in loss of phosphorylation of MAPK8IP3; when associated with A-266 and A-287. Does not effect binding of components of the JNK pathway.
Results in loss of phosphorylation of MAPK8IP3; when associated with A-276 and A-287. Does not effect binding of components of the JNK pathway.
BPTI/Kunitz inhibitor domain-containing protein
Abolished activity without affecting avility to bind CX3CL1.
In DR/NN; abolished activity without affecting avility to bind CX3CL1.
Mitochondrial potassium channel ATP-binding subunit
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
Fat storage-inducing transmembrane protein 1
Mice show an atypical limb development, are covered by an abnormal smooth skin and die shortly after birth. The esophageal lumen is obliterated by adhesion of the stratified, squamous epithelium lining this structure. The hindlimbs, tail and body are fused together by a thickened epidermis. Epidermis is hyperproliferative but does not undergo a normal differentiation program.
N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
Ubiquitin carboxyl-terminal hydrolase 10
Nucleoside diphosphate kinase, mitochondrial
Serine/threonine-protein phosphatase 2A activator
Phosphotyrosine; by PKMYT1, WEE1, WEE2 and PKC/PRKCD
Important for interaction with 14-3-3 proteins
Loss of phosphorylation site. Strongly reduced interaction with YWHAG and YWHAB.
Abolishes guanine nucleotide exchange factor (GEF) activity and impaired TCR down-modulation and recycling in TH2 cells.
Impaired interaction with the AP-2 complex and impaired TCR down-modulation and recycling in TH2 cells; when associated with 547-A--A-549.
Impaired interaction with the AP-2 complex and impaired TCR down-modulation and recycling in TH2 cells; when associated with A-482.
Heparan sulfate glucosamine 3-O-sulfotransferase 1
Strongly decrease the enzymatic activity.
Strongly decreases the enzymatic activity.
NADP-dependent malic enzyme, mitochondrial
Peptidyl-prolyl cis-trans isomerase FKBP14
Does not inhibit calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-232 and N-234.
Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-39 and N-97.
Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-39 and N-99.
Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-97 and N-99.
Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-39; N-97 and N-99.
Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-176 and N-232.
Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-176 and N-234.
Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-176; N-232 and N-234.
Interferon-induced transmembrane protein 2
Guanine nucleotide-binding protein-like 1
Cyclic AMP-responsive element-binding protein 3-like protein 4
Processed cyclic AMP-responsive element-binding protein 3-like protein 4
Abolishes ability to deubiquitinate histone H2A and ability to regulate transcription.
Ubiquitin carboxyl-terminal hydrolase 21
Abolishes interaction with RORC; when associated with 644-A-A-645 and 689-A--A-694.
Abolishes interaction with RORC; when associated with 644-A-A-645 and 744-A--A-749.
Abolishes interaction with RORC; when associated with 689-A--A-694 and 744-A--A-749.
Abolishes phosphorylation at Ser-391. Abolishes interaction with PPP2CA and PPP2R1A.
Abolishes phosphorylation. Abolishes interaction with PPP2CA and PPP2R1A. Slight increase in phosphorylation at Ser-273 and in the interaction with 14-3-3 proteins.
CREB-regulated transcription coactivator 3
Does not affect phosphorylation at Ser-391 or the interaction with PPP2CA and PPP2R1A.
Reduces interaction with 14-3-3 proteins without affecting the interaction with PPP2R1A; when associated with A-329 and A-370.
Reduces interaction with 14-3-3 proteins without affecting the interaction with PPP2R1A; when associated with A-62 and A-329.
Reduces interaction with 14-3-3 proteins without affecting the interaction with PPP2R1A; when associated with A-62 and A-370.
Reduces interaction with 14-3-3 proteins. Abolishes interaction with 14-3-3 proteins without affecting the interaction with PPP2R1A; when associated with A-162.
Up-regulates CREB transcription factor activity. Reduces interaction with 14-3-3 proteins. Abolishes interaction with 14-3-3 proteins without affecting the interaction with PPP2R1A; when associated with A-273.
Leucine-rich repeat-containing protein 19
Cyclic AMP-responsive element-binding protein 3-like protein 1
Loss of proteolytic cleavage; when associated with L-392 and A-423.
Loss of proteolytic cleavage; when associated with L-392 and V-426.
Loss of proteolytic cleavage; when associated with V-423 and V-426.
Processed cyclic AMP-responsive element-binding protein 3-like protein 1
Voltage-gated potassium channel subunit beta-1
Zinc finger SWIM domain-containing protein 3
Impairs binding to and processing of 5.8S rRNA; when associated with A-107.
Impairs binding to and processing of 5.8S rRNA; when associated with A-108.
No effect on binding to 5.8S rRNA; when associated with G-130.
No effect on binding to 5.8S rRNA; when associated with G-132.
Glucocorticoid modulatory element-binding protein 1
Outer dense fiber protein 3-like protein 1
Sperm acrosome membrane-associated protein 3
Sperm acrosome membrane-associated protein 3, membrane form
Sperm acrosome membrane-associated protein 3, processed form
Abolishes binding to TELO2-phosphopeptide.
No effect on binding to TELO2-phosphopeptide.
N-acetylthreonine; in GTPase KRas, N-terminally processed
Abolishes DNA-binding. No effect on transcriptional repression.
Decreased phosphorylation by GSK3A and interaction with 14-3-3 proteins.
Decreases DNA-binding. No effect on transcriptional repression.
39S ribosomal protein L44, mitochondrial
Heterogeneous nuclear ribonucleoprotein D0
Eukaryotic translation initiation factor 2A
Eukaryotic translation initiation factor 2A, N-terminally processed
N-acetylalanine; in Eukaryotic translation initiation factor 2A, N-terminally processed
Ubiquitin/ISG15-conjugating enzyme E2 L6
NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
Cysteine and histidine-rich domain-containing protein 1
Germinal-center associated nuclear protein
Reduced tyrosine phosphorylation. Abolished ability to mediate recruitment of PTPN11/SHP-2.
Reduced tyrosine phosphorylation. Decreased ability to mediate recruitment of PTPN11/SHP-2.
Acid sphingomyelinase-like phosphodiesterase 3a
Myosin regulatory light chain 2, atrial isoform
Rho guanine nucleotide exchange factor 1
Zinc finger BED domain-containing protein 6
CCR4-NOT transcription complex subunit 9
Abolishes the nuclear speckle localization but not the nuclear localization.
Does not affect the subcellular localization.
N-acetylneuraminate 9-O-acetyltransferase
Myb/SANT-like DNA-binding domain-containing protein 3
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
Increases pain sensitivity; reduces threshold for induction of cortical spreading depression; increases arterial dilation during cortical spreading depression and increases spontaneous and evoked calcium signaling in astrocytes.
Rho guanine nucleotide exchange factor 19
Short transient receptor potential channel 4
GTP-binding nuclear protein Ran, testis-specific isoform
Interchain (with C-92 in IL12A and C-74 in IL23A)
tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
Gamma-aminobutyric acid receptor subunit alpha-4
Abolishes enzyme activity. Unable to mediate serine depalmitoleoylation of WNT proteins.
Palmitoleoyl-protein carboxylesterase NOTUM
Polypeptide N-acetylgalactosaminyltransferase 13
Transcription initiation factor TFIID subunit 13
Cell division cycle-associated protein 2
N-acetylglucosamine-6-phosphate deacetylase
Proprotein convertase subtilisin/kexin type 7
Peroxisome proliferator-activated receptor delta
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Coxsackievirus and adenovirus receptor homolog
Disintegrin and metalloproteinase domain-containing protein 32
Loss of membrane localization; when associated with Q-698; Q-699; Q-700 and Q-705.
Loss of membrane localization; when associated with Q-698; Q-699; Q-700 and Q-706.
Loss of membrane localization; when associated with Q-698; Q-699; Q-705 and Q-706.
Loss of membrane localization; when associated with Q-698; Q-700; Q-705 and Q-706.
Loss of membrane localization; when associated with Q-699; Q-700; Q-705 and Q-706.
Patatin-like phospholipase domain-containing protein 5
Abolishes interaction with TBK1 and IKBKE.
Abolishes interaction with TBK1 but not with IKBKE.
No effect on interaction with TBK1 and IKBKE.
Abolishes phosphorylation by CAMK2A. Loss of transcriptional activity.
Results in enlarged multinucleated cells.
Shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number.
Does not affect formation of a heterodimer with TCF3 and ability to bind DNA.
Impaired formation of a heterodimer with TCF3 and decreased ability to bind DNA.
Uveal autoantigen with coiled-coil domains and ankyrin repeats
Intraflagellar transport protein 52 homolog
TATA box-binding protein-associated factor RNA polymerase I subunit C
Asparagine synthetase domain-containing protein 1
Loss of sumoylation; when associated with R-724.
Loss of sumoylation; when associated with R-742.
Transcription intermediary factor 1-alpha
Glycerol-3-phosphate dehydrogenase, mitochondrial
Abolishes formation of tight-junction strand-like structures.
Abolishes palmitoylation; when associated with 183-A--A-185.
Abolishes palmitoylation; when associated with A-102.
No effect on formation of tight-junction strand-like structures.
Signal transducer and transcription activator 6
Leucine zipper transcription factor-like protein 1
Change of cotransport Na(+):Pi stoichiometry to 3:1; when associated with 189-AGA-191.
Change of cotransport Na(+):Pi stoichiometry to 3:1; when associated with D-195.
Sodium-dependent phosphate transport protein 2C
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
BRD4-interacting chromatin-remodeling complex-associated protein
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
V-type proton ATPase 116 kDa subunit a 2
Loss of ability to induce formation of neurite-like membrane protrusions in non-neuronal cells.
Reduced nuclear export; when associated with A-249.
Reduced nuclear export; when associated with A-251.
Reduced nuclear export; when associated with A-272.
Reduced nuclear export; when associated with A-274.
Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]
Pterin-4-alpha-carbinolamine dehydratase 2
Distal membrane-arm assembly complex protein 2
Uncharacterized protein C16orf90 homolog
Protein-glutamine gamma-glutamyltransferase 5
Abolishes nuclear pore localization; when associated with 272-GSGSGSGSG-280.
Abolishes nuclear pore localization; when associated with S-284.
Rab3 GTPase-activating protein non-catalytic subunit
Calcium/calmodulin-dependent protein kinase type II subunit delta
Coiled-coil domain-containing protein 174
Cilia- and flagella-associated protein 65
No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-176.
No reduction of association with the recycling endosome membranes and does activate TNIK; when associated with G-177.
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Loss of stimulation of rRNA synthesis. Loss of protein synthesis promotion. No effect on interaction with DDX3X, EIF3G, EIF3H and RPL27.
Abolished mono-ADP-ribosylation by SIRT6 and recruitment to the enhancer region of the Heme oxygenase-1 (HO-1) locus.
LETM1 domain-containing protein LETM2, mitochondrial
ATP synthase subunit epsilon, mitochondrial
Probable arginine--tRNA ligase, mitochondrial
Mitochondrial import receptor subunit TOM70
Kinase non-catalytic C-lobe domain-containing protein 1
Aldehyde dehydrogenase family 3 member B2
LHFPL tetraspan subfamily member 1 protein
Receptor-type tyrosine-protein phosphatase
Receptor-type tyrosine-protein phosphatase epsilon
Solute carrier family 2, facilitated glucose transporter member 3
Barrier-to-autointegration factor, N-terminally processed
N-acetylthreonine; in Barrier-to-autointegration factor, N-terminally processed
CCR4-NOT transcription complex subunit 6
Disrupts interaction of the first PDZ domain with SCARB1. Abolishes interaction with SCARB1; when associated with A-253.
Disrupts interaction of the third PDZ domain with SCARB1. Abolishes interaction with SCARB1; when associated with A-20.
Impairs interaction of the first PDZ domain with SCARB1.
Na(+)/H(+) exchange regulatory cofactor NHE-RF3
39S ribosomal protein L24, mitochondrial
Zinc finger CCCH domain-containing protein 3
Transmembrane and coiled-coil domains protein 2
RNA-binding motif, single-stranded-interacting protein 3
Complement C1q tumor necrosis factor-related protein 1
Ubiquitin carboxyl-terminal hydrolase 17-like protein B
No effect on binding to SIVA1; when associated with G-204.
No effect on binding to SIVA1; when associated with R-201.
Tumor necrosis factor receptor superfamily member 18
Vacuolar protein sorting-associated protein 4A
EP300-interacting inhibitor of differentiation 1
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2
Strongly reduced activity at low pH, with minor effect on activity at neutral pH.
Ankyrin repeat domain-containing protein 37
11-beta-hydroxysteroid dehydrogenase type 2
Armadillo-like helical domain-containing protein 3
Uncharacterized protein C16orf46 homolog
Inactive peptidyl-prolyl cis-trans isomerase FKBP6
Splicing factor, proline- and glutamine-rich
tRNA-uridine aminocarboxypropyltransferase 2
Histone-lysine N-methyltransferase SETD2
Disrupts LDB1-binding; when associated with G-28.
Disrupts LDB1-binding; when associated with G-88.
X-linked lymphocyte-regulated protein 3B
Acidic leucine-rich nuclear phosphoprotein 32 family member E
reversibly protonated residue during proton transport
Mitochondrial basic amino acids transporter
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
Translational activator of cytochrome c oxidase 1
Dehydrogenase/reductase SDR family member 7
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Alpha-aminoadipic semialdehyde synthase, mitochondrial
Voltage-dependent calcium channel gamma-6 subunit
Cation channel sperm-associated protein 4
Heterogeneous nuclear ribonucleoprotein K
Eukaryotic translation initiation factor 2D
No increase in phosphorylation of AKT1, FOXO1 and INSR. No increased expression of SLC2A1, FABP4 nor ADIPOQ. Impaired formation of large lipid droplets.
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
Calcium-binding and spermatid-specific protein 1
Abolishes N-terminal proteolytic processing.
Abolishes the nuclear localization; when associated with G-101.
Abolishes the nuclear localization; when associated with G-109.
Cytoplasmic dynein 2 intermediate chain 2
Selection and upkeep of intraepithelial T-cells protein 4
Proton acceptor; for processing activity
S-adenosylmethionine decarboxylase 2 alpha chain
S-adenosylmethionine decarboxylase 2 beta chain
Schiff-base intermediate with substrate; via pyruvic acid
Leucine-rich repeat-containing protein 4C
Rho guanine nucleotide exchange factor 33
Transient receptor potential cation channel subfamily M member 1
Heterogeneous nuclear ribonucleoprotein U
Gamma-aminobutyric acid receptor subunit beta-3
Mainly expressed in the cytoplasm; when associated with A-7 and A-10.
Mainly expressed in the cytoplasm; when associated with A-7 and A-8.
Mainly expressed in the cytoplasm; when associated with A-8 and A-10.
Mitochondrial tRNA-specific 2-thiouridylase 1
26S proteasome non-ATPase regulatory subunit 5
Ethanolamine-phosphate cytidylyltransferase
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
28S ribosomal protein S15, mitochondrial
Biogenesis of lysosome-related organelles complex 1 subunit 6
In strain: BALB/c, CBA and C57BL/6J-pa mutants.
Kelch repeat and BTB domain-containing protein 12
Alters RRM 1 stability. Abolishes regulation of alternative splicing.
Heterogeneous nuclear ribonucleoprotein L-like
Dehydrodolichyl diphosphate synthase complex subunit Nus1
RXG motif; crucial for prenyltransferase activity
Abolishes phosphorylation and ability to regulate cytoskeletal organization; when associated with F-103.
Abolishes phosphorylation and ability to regulate cytoskeletal organization; when associated with F-92.
Failure to rescue cytoskeletal defects when introduced into TYROBP-deficient cells.
TYRO protein tyrosine kinase-binding protein
Connector enhancer of kinase suppressor of ras 3
Guanine nucleotide-binding protein subunit beta-4
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1
Ankyrin repeat domain-containing protein 13B
NEDD8-activating enzyme E1 catalytic subunit
In XID; prevents interaction with ARID3A.
Loss of activity and no phosphorylation.
Lysosomal cobalamin transport escort protein LMBD1
Significantly reduced interaction with adapter protein complex 2 and disruption of its function in clathrin-mediated endocytosis of INSR.
WTKF motif; mediates interaction with adapter protein complex 2 and is essential for its function in clathrin-mediated endocytosis of INSR
YERL motif; mediates interaction with adapter protein complex 2 and is essential for its function in clathrin-mediated endocytosis of INSR
Serine/threonine-protein phosphatase 6 regulatory subunit 1
Translation initiation factor eIF-2B subunit epsilon
Uncharacterized protein C17orf98 homolog
Baculoviral IAP repeat-containing protein 2
Abolishes DNA-binding. Does not affect ability to activate expression of CDKN2A.
DNA-directed RNA polymerase I subunit RPA2
Trimeric intracellular cation channel type A
Afadin- and alpha-actinin-binding protein
General transcription factor IIF subunit 2
Tumor necrosis factor alpha-induced protein 8-like protein 1
Abolished serotonylation by TGM2. Reduced neurite length.
Calmodulin-regulated spectrin-associated protein 1
39S ribosomal protein L15, mitochondrial
Nuclear protein localization protein 4 homolog
Ribonucleoside-diphosphate reductase large subunit
Normal response to light but reduced retinal electrical activity in response to light in dark adapted retinas. Gross morphology is normal.
Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A
Brefeldin A-inhibited guanine nucleotide-exchange protein 3
Cilia- and flagella-associated protein 97
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Cytochrome c oxidase assembly protein COX19
Pleckstrin homology domain-containing family G member 3
Abolished ubiquitination and degradation by the BCR(KEAP1) complex in the cytoplasm.
Abolished ubiquitination and degradation by the BCR(KEAP1) complex in the cytoplasm; when associated with 19-A--A-23.
Abolished ubiquitination and degradation by the BCR(KEAP1) complex in the cytoplasm; when associated with A-30.
Does not affect ubiquitination and degradation by the BCR(KEAP1) complex in the cytoplasm.
Nuclear factor erythroid 2-related factor 2
TOX high mobility group box family member 3
DNA-directed RNA polymerase III subunit RPC2
Protein transport protein Sec61 subunit beta
Tumor necrosis factor receptor superfamily member 27
Testis-specific Y-encoded-like protein 1
POU domain, class 2, transcription factor 2
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3
V-type proton ATPase 116 kDa subunit a 1
Serine-rich coiled-coil domain-containing protein 2
Abrogates interaction with NDEL1, PAFAH1B3 and RSN. Also impairs localization to centrosomes and microtubule plus ends. Reduces protein stability.
Abrogates interaction with NDLE1, NUDC, PAFAH1B3 and RSN. Also impairs localization to centrosomes and microtubule plus ends. Reduces protein stability.
Abrogates interaction with PAFAH1B3 and RSN. Also impairs localization to centrosomes and microtubule plus ends. Reduces protein stability.
Abrogates self-association and interaction with DAB1, dynein, NDEL1, PAFAH1B3 and RSN. Also impairs localization to centrosomes and microtubule plus ends. Reduces protein stability.
Impairs self-association and reduces protein stability.
Impairs self-association, reduces protein stability and promotes localization to actin fibers.
Platelet-activating factor acetylhydrolase IB subunit beta
N-alpha-acetyltransferase 35, NatC auxiliary subunit
Leucine-rich repeat-containing protein 71
Vacuolar protein sorting-associated protein 8 homolog
When overexpressed ubiquitously in conditional knockin mice, perinatally lethal; when overexpressed in osteoblasts in conditional knockin mice, produces a severe skeletal phenotype marked by a drastic increase in cortical thickness due to an enhanced endosteal bone formation; when overexpressed in osteoclasts in conditional knockin mice, no effect on bone hemostasis.
Zinc finger CCHC domain-containing protein 7
Mediator of RNA polymerase II transcription subunit 1
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Ganglioside-induced differentiation-associated protein 2
Sterile alpha motif domain-containing protein 5
Strongly reduced binding affinity for EPHA5 SAM domain.
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
Abolishes interaction with WWP2; if associated with F-174.
Abolishes interaction with WWP2; if associated with F-208.
Reduces transcriptional regulatory activity.
Tumor necrosis factor ligand superfamily member 10
Potassium voltage-gated channel subfamily E member 4
Probable constitutively active mutant unable to hydrolyze GTP; stimulates translocation of SLC2A4 to the plasma membrane.
Acidic fibroblast growth factor intracellular-binding protein
Impairs LCFA-mediated phosphorylation and interaction with ARRB2.
C5a anaphylatoxin chemotactic receptor 1
Rab proteins geranylgeranyltransferase component A 2
Isocitrate dehydrogenase [NADP] cytoplasmic
No effect on inhibition by cadmium ions.
Loss of interaction with PPP1R12C and PPP1R12A.
Myosin phosphatase Rho-interacting protein
Beta-galactoside alpha-2,6-sialyltransferase 1
SPRY domain-containing SOCS box protein 4
Thyroid transcription factor 1-associated protein 26
Urokinase plasminogen activator surface receptor
GDH/6PGL endoplasmic bifunctional protein
5'-AMP-activated protein kinase catalytic subunit alpha-2
Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and in neurons; when associated with F-1416.
Slight reduction of phosphorylation in HEK293T cells. Abolishes phosphorylation in HEK293T cells and neurons; when associated with F-1441.
Abolishes homooligomerization and FLRT3-mediated cell-cell adhesion; when associated with N-181.
Abolishes homooligomerization and FLRT3-mediated cell-cell adhesion; when associated with T-183.
Leucine-rich repeat transmembrane protein FLRT3
Coiled-coil domain-containing protein 34
Coiled-coil domain-containing protein 93
Calcium-activated chloride channel regulator 1
Uncharacterized protein C1orf185 homolog
ATP-binding cassette sub-family C member 6
G-protein coupled receptor family C group 6 member A
No effect on channel activation kinetics.
28S ribosomal protein S18a, mitochondrial
Myb-related transcription factor, partner of profilin
Reduction in homodimerization. Abolishes homodimerization; when associated with A-281.
Reduction in homodimerization. Abolishes homodimerization; when associated with P-281.
Centrosomal protein of 170 kDa protein B
Single-pass membrane and coiled-coil domain-containing protein 2
Prevents the correct removal of the propeptide.
In SG; disturbance of Purkinje cell and muscle development, lipid metabolism, circadian behavior and immune system functioning.
Glucose-fructose oxidoreductase domain-containing protein 2
Uncharacterized aarF domain-containing protein kinase 2
Caspase recruitment domain-containing protein 10
Stabilizes the protein, 17-fold lower Vmax.
Almost no change in fusion-facilitating activity.
O-linked (Xyl...) (dermatan sulfate) serine
Apoptosis facilitator Bcl-2-like protein 14
U3 small nucleolar RNA-associated protein 25 homolog
Aryl hydrocarbon receptor nuclear translocator 2
Compromises SIM1:ARNT2 heterodimer stability. Compromises NPAS4:ARNT2 heterodimer stability.
Compromises SIM1:ARNT2 heterodimer stability. Does not compromises NPAS4:ARNT2 heterodimer stability.
CXXC motif containing zinc binding protein
Sodium/potassium-transporting ATPase subunit beta-1
Abolishes phosphatase activity. Abolishes phosphatase activity; when associated with S-67 and R-72. Strongly reduces phosphatase activity; when associated with S-67; R-71 and R-72. Mildly reduces phosphatase activity; when associated with R-71 and R-72.
Mildly reduces phosphatase activity; when associated with R-71 and A-73. Strongly reduces phosphatase activity; when associated with S-67; R-71 and A-73. Abolishes phosphatase activity; when associated with S-67 and A-73.
Mildly reduces phosphatase activity; when associated with R-72 and A-73. Strongly reduces phosphatase activity; when associated with S-67; R-72 and A-73.
Slightly increases phosphatase activity with p-nitrophenylphosphate; when associated with N-41 and I-45.
Slightly increases phosphatase activity with p-nitrophenylphosphate; when associated with N-41 and R-44.
Slightly increases phosphatase activity with p-nitrophenylphosphate; when associated with R-44 and I-45.
Strongly increases activity with pyridoxal phosphate.
Strongly reduces phosphatase activity; when associated with R-71; R-72 and A-73. Abolishes phosphatase activity; when associated with R-72 and A-73.
Loss of interaction with clathrin heavy chains; when associated with 187-AAAAA-191.
Loss of interaction with clathrin heavy chains; when associated with 212-AAA-214.
Dehydrogenase/reductase SDR family member 4
Cytoplasmic dynein 2 intermediate chain 1
EIF4E-binding reduced by 20%; when associated with A-724.
EIF4E-binding reduced by 20%; when associated with A-726.
EIF4E-binding reduced by 60%; when associated with E-725, E-726 and K-730.
EIF4E-binding reduced by 60%; when associated with K-724, E-725 and E-726.
EIF4E-binding reduced by 60%; when associated with K-724, E-725 and K-730.
EIF4E-binding reduced by 70%. EIF4E-binding reduced by 60%; when associated with K-724, E-726 and K-730.
Guanine nucleotide-binding protein G(q) subunit alpha
Golgi-associated RAB2 interactor protein 1B
Required for drug-induced death protein 1
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
Microtubule-associated tumor suppressor 1 homolog
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Endosome-associated-trafficking regulator 1
Processed beta-1,4-galactosyltransferase 1
28S ribosomal protein S31, mitochondrial
In strain: BALB/cByJ and C57BL/6J; requires 2 nucleotide substitutions.
Phosphofurin acidic cluster sorting protein 2
In isoform 7, isoform 12 and isoform 13.
Loss of interaction with calmodulin; when associated with E-525.
Loss of interaction with calmodulin; when associated with E-526.
Potassium voltage-gated channel subfamily KQT member 2
SAM pointed domain-containing Ets transcription factor
Mitochondrial inner membrane protease subunit 1
F-box-like/WD repeat-containing protein TBL1XR1
Significantly decreases interaction with MECP2.
Substantially reduces interaction with MECP2.
Weakly decreases interaction with MECP2.
Fibroblast growth factor-binding protein 1
Nuclear receptor subfamily 4 group A member 1
Phosphoserine; by PKA, RPS6KA1 and RPS6KA3
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
PWWP domain-containing DNA repair factor 3B
Uncharacterized protein C11orf91 homolog
Uncharacterized serine/threonine-protein kinase SBK3
Constitutive coactivator of PPAR-gamma-like protein 1
Protein N-terminal asparagine amidohydrolase
Integrin_alpha2 domain-containing protein
Does not affect interaction with MAPK1. Loss of interaction with MAPK1; when associated with A-62.
Does not affect phosphatase activity. Loss of phosphatase activity; when associated with A-294 and A-295. Does not affect interaction with MAPK1.
Loss of interaction with MAPK1. Loss of interaction with MAPK1; when associated with A-61.
Loss of phosphatase activity. Does not affect interaction with MAPK1.
Loss of phosphatase activity. Loss of phosphatase activity; when associated with A-292 and A-294. Does not affect interaction with MAPK1.
Loss of phosphatase activity. Loss of phosphatase activity; when associated with A-292 and A-295. Does not affect interaction with MAPK1.
Abolishes deglutamylase activity; when associated with Q-255.
Abolishes deglutamylase activity; when associated with S-252.
Cytosolic carboxypeptidase-like protein 5
GTPase-activating Rap/Ran-GAP domain-like protein 3
Eukaryotic initiation factor 4A-III, N-terminally processed
N-acetylalanine; in Eukaryotic initiation factor 4A-III, N-terminally processed
Calcium-activated potassium channel subunit beta-2
4S motif; modulates transactivation activity and protein stability
Reduced phosphorylation. Does not affect nuclear localization.
Potassium voltage-gated channel subfamily G member 3
Coiled-coil domain-containing protein 172
U6 snRNA-associated Sm-like protein LSm1
Abolishes in vitro binding to 'Lys-10' methylated H3, H3K9me3. Prevents enrichment at pericentric heterochromatin.
Abolishes in vitro binding to H3K9me3. Prevents enrichment at pericentric heterochromatin.
Signaling threshold-regulating transmembrane adapter 1
Formyl peptide receptor-related sequence 3
Coiled-coil domain-containing protein 175
Armadillo repeat-containing X-linked protein 2
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
Minichromosome maintenance domain-containing protein 2
Zinc finger and SCAN domain containing protein 4C
Glycolipid transfer protein domain-containing protein 2
Suppressor of tumorigenicity 7 protein-like
Abolishes sumoylation. Abolishes sumoylation; when associated with R-868 and R-872.
No change in sumoylation. Abolishes sumoylation; when associated with R-868 and R-882.
No change in sumoylation. Abolishes sumoylation; when associated with R-872 and R-882.
Neuronal acetylcholine receptor subunit alpha-2
Mitochondrial peptide methionine sulfoxide reductase
Phosphoserine; by IKKA, IKKB, IKKE and TBK1
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Ribonuclease P protein subunit p25-like protein
tRNA (guanine(26)-N(2))-dimethyltransferase
Abolished HIPK2-mediated proteasomal degradation.
Cysteinyl-selenocysteine (Cys-Sec); redox-active
Impairs protection against hydrogen peroxide-induced toxicity.
Increased sensitivity to hydrogen peroxide-induced toxicity.
Transcription initiation factor TFIID subunit 6
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Homeodomain-interacting protein kinase 1
Increases basal and ligand-induced adipogenic activity. Abolishes repression by PER2 on transactivation activity.
No effect on repression by PER2 on transactivation activity.
Peroxisome proliferator-activated receptor gamma
BMP and activin membrane-bound inhibitor homolog
Knockin mice show impaired spermatogenesis, loss of histone H3K4me3 binding, severe disruption of chromosomal synapsis and DNA double-strand breaks repair in spermatocytes; when associated with I-247 and P-294.
Knockin mice show impaired spermatogenesis, loss of histone H3K4me3 binding, severe disruption of chromosomal synapsis and DNA double-strand breaks repair in spermatocytes; when associated with I-247 and R-292.
Knockin mice show impaired spermatogenesis, loss of histone H3K4me3 binding, severe disruption of chromosomal synapsis and DNA double-strand breaks repair in spermatocytes; when associated with R-292 and P-294.
Zinc finger CW-type PWWP domain protein 1
Cytochrome c oxidase subunit 7A1, mitochondrial
Dipeptidyl peptidase 1 exclusion domain chain
Homozygous mutant mice show signs of hepatocytes degeneration associated with elevated serum levels of total bile acid, alanine transaminase and aspartate transaminase. Levels of the canalicular bile acid transporters ABCB11 and ABCC2 are reduced.
Serine/threonine-protein kinase 24 12 kDa subunit
Serine/threonine-protein kinase 24 35 kDa subunit
T-cell immunoglobulin and mucin domain-containing protein 2
Abolishes interaction with MYD88 and IL-1-dependent activation of NF-kappa-B.
Abolishes interaction with PTPRD; when associates with 69-A--A-71 Significantly reduces synaptogenesis; when associates with 82-A--A-85.
Abolishes interaction with PTPRD; when associates with 82-A--A-85. Significantly reduces synaptogenesis; when associates with 82-A--A-85.
Interleukin-1 receptor accessory protein
V-set and immunoglobulin domain-containing protein 1
Lipopolysaccharide-responsive and beige-like anchor protein
Abolished recognition by the CRL2(FEM1B) complex and subsequent ubiquitination.
Does not affect recognition by the CRL2(FEM1B) complex and subsequent ubiquitination.
CCR4-NOT transcription complex subunit 11
No effect on chiting-binding. No restoration of chitinase activity.
DNA methyltransferase 1-associated protein 1
Mas-related G-protein coupled receptor member A2
No phosphorylation by AMPK, reduced fatty acid oxidation in skeletal muscle, increased lipid deposition in skeletal muscle, and development of insulin resistance.
Does not affect phosphorylarion at Ser-41; when associated with A-195.
Does not affect phosphorylarion at Ser-41; when associated with R-219.
Abolishes phosphorylation, disrupts interaction with PIK3R1 and PIK3R2, no LGALS9-mediated disruption of interaction with BAG6; when associated with F-256.
Abolishes phosphorylation, disrupts interaction with PIK3R1 and PIK3R2, no LGALS9-mediated disruption of interaction with BAG6; when associated with F-263.
Decreases phosphatidylserine binding, abolishes phagocytic activity.
Greatly decreases phosphatidylserine binding.
Hepatitis A virus cellular receptor 2 homolog
Oxysterol-binding protein-related protein 9
ATP-binding cassette sub-family F member 3
Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D
N-acetyllactosaminide alpha-1,3-galactosyltransferase
Inhibits binding to PIP2 and disrupts membrane binding.
Does not affect ceramide synthase activity.
In toppler (to) mice; impairs ceramide synthase activity.
Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+)
General transcription factor IIH subunit 4
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
In an experimental autoimmune encephalomyelitis (EAE) disease model, 50 percent of animals have no EAE symptoms. In the remaining animals, EAE is less severe with a reduction in the demyelination of the spinal cord and in the number of CD4(+) T-cells infiltrating the central nervous system. In addition, CD4(+) T-cell proliferation and apoptosis are increased. Reduced CD4(+) T-cell transmigration towards chemokine CXCL12 and CCL5.
Mas-related G-protein coupled receptor member H
Ribosome-recycling factor, mitochondrial
Tyrosine-protein phosphatase non-receptor type 4
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Probable phospholipid-transporting ATPase IIB
Phosphothreonine; by MTOR, NEK6 and NEK7
WAS/WASL-interacting protein family member 2
Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 3
Kinase-dead. Loss of autophosphorylation but has no effect on regulation of cell adhesion.
Reduced phosphorylation and reduced association with FYN.
Phosphothreonine; by MAP2K3, MAP2K4, MAP2K6 and MAP2K7
Fails to form disulfide bonds and to induce filopodia-like protrusions.
Leucine zipper putative tumor suppressor 2
Actin-related protein 2/3 complex subunit 5-like protein
ATP synthase subunit beta, mitochondrial
Pyridoxal phosphate phosphatase PHOSPHO2
Abolishes interaction with CTNNB1. No effect on nuclear localization.
BTB/POZ domain-containing protein KCTD11
Major facilitator superfamily domain-containing protein 10
Gamma-aminobutyric acid receptor subunit delta
Zinc finger protein with KRAB and SCAN domains 1
Glutathione-specific gamma-glutamylcyclotransferase 1
Loss of catalytic activity against glutathione.
Loss of SLC9A3R2 binding and reduced nuclear localization. Partial recovery of nuclear accumulation; when associated with A-89.
Loss of YWHAZ binding with increased nuclear localization. Partial recovery of nuclear accumulation; when associated with deletion of 392-L--L-395.
WW domain-containing transcription regulator protein 1
Decrease in cytidine deaminase and antiviral activity.
Decrease in cytidine deaminase and antiviral activity; when associated with A-301.
Decrease in cytidine deaminase and antiviral activity; when associated with A-84.
In cell line MDTF; requires 2 nucleotide substitutions.
No effect on cytidine deaminase and antiviral activity.
Intraflagellar transport protein 88 homolog
Impaired ability to associate with CGAS-DNA droplets.
Loss of activity. Loss of endogenous retroelement metabolization.
Reduces activity. Loss of endogenous retroelement metabolization.
Increased degradation by the proteasome and a decrease in isoform 2/deltaFosB transactivation activity.
Broad substrate specificity ATP-binding cassette transporter ABCG2
Band 4.1-like protein 3, N-terminally processed
In isoform 2, isoform 3, isoform 5, isoform 6 and isoform 7.
In isoform 4, isoform 5, isoform 6, isoform 7 and isoform 8.
N-acetylthreonine; in Band 4.1-like protein 3, N-terminally processed
Important for ABL1-mediated Tyr-718 phosphorylation
Strong decrease in catalytic activity; when associated with A-707.
Strong decrease in catalytic activity; when associated with A-711.
Regulator of microtubule dynamics protein 1
In isoform 3, isoform 5, isoform 6, isoform 8 and isoform 11.
Platelet-activating factor acetylhydrolase IB subunit alpha1
Potassium voltage-gated channel subfamily S member 3
Forms homodimers processed at L-112 that seem more stable than the wild-type.
Reduced hippocampal volume, dendritic complexity and context-dependent memory. Decreased density of dendritic mushroom spines and synapses. Impaired fear extinction. Increased anxiety-related behaviors.
Protein N-lysine methyltransferase METTL21D
Splicing factor, suppressor of white-apricot homolog
Abolished ability to ubiquitinate NFE2L2/NRF2 without affecting homodimerization.
Abolishes repression of NFE2L2/NRF2-dependent gene expression.
Abolishes repression of NFE2L2/NRF2-dependent gene expression. Knockin mice die approximately three weeks after birth because of impaired ability of the BCR(KEAP1) complex to ubiquitinate NFE2L2/NRF2; when associated with A-288.
Abolishes repression of NFE2L2/NRF2-dependent gene expression. Slows down degradation of NFE2L2/NRF2. Knockin mice die approximately three weeks after birth because of impaired ability of the BCR(KEAP1) complex to ubiquitinate NFE2L2/NRF2; when associated with A-273.
Decreases repression of NFE2L2/NRF2-dependent gene expression.
Does not affect interaction with SQSTM1/p62. Abolished interaction with SQSTM1/p62; when associated with A-380 and A-415.
Impaired interaction with SQSTM1/p62. Abolished interaction with SQSTM1/p62; when associated with A-380 and A-483.
Impaired interaction with SQSTM1/p62. Abolished interaction with SQSTM1/p62; when associated with A-415 and A-483.
N5-[4-(S-L-cysteinyl)-5-methyl-1H-imidazol-2-yl]-L-ornithine (Arg-Cys) (interchain with C-151 in KEAP1)
N5-[4-(S-L-cysteinyl)-5-methyl-1H-imidazol-2-yl]-L-ornithine (Cys-Arg) (interchain with R-135 in KEAP1)
Retains ability to degrade NFE2L2/NRF2; when associated with E-288.
Retains ability to degrade NFE2L2/NRF2; when associated with W-273. Abolishes ability to be activated by 15-deoxy-delta(12,14)-prostaglandin J2.
S-(2,3-dicarboxypropyl)cysteine; alternate
Substitution with a small side chain that prevents covalent modification by an electrophile; promotes constitutive ubiquitination of NFE2L2/NRF2 and subsequent repression of phase 2 detoxifying enzymes. Knockin mice are healthy and viable in normal conditions but do not activate NFE2L2/NRF2 in response to oxidative stress.
Fibroblast growth factor receptor-like 1
Mitochondrial ribonuclease P catalytic subunit
A disintegrin and metalloproteinase with thrombospondin motifs 12
Pep_M12B_propep domain-containing protein
Carbamoyl-phosphate synthase [ammonia], mitochondrial
Reduces interaction with EPS15 and SYNRG.
Strongly reduces interaction with EPS15 and SYNRG.
Heparan sulfate glucosamine 3-O-sulfotransferase 5
von Willebrand factor A domain-containing protein 5B1
Causes local perturbations of protein folding; when associated with T-122.
Abolished guanosine exchange factor (GEF) activity for Ran.
Abolishes repression of germ cell-related genes and L1 retrotransposons.
ATP-dependent 6-phosphofructokinase, muscle type
Sodium-dependent organic anion transporter
Potassium voltage-gated channel subfamily A member 3
Abolishes association with microtubules and the ability to promote microtubule polymerization.
Abolishes association with microtubules.
Expected to disrupt microtubule binding. Abolishes the ability to promote microtubule polymerization.
Resulty in increased tubulin polymerization in vitro.
TOG array regulator of axonemal microtubules protein 1
Voltage-dependent L-type calcium channel subunit beta-3
Dominant-negative mutant that induces defects in membrane repair.
Results in aberrent localization of TRIM72 and defects in membrane repair.
Ribosome biogenesis protein NSA2 homolog
Bifunctional peptidase and arginyl-hydroxylase JMJD5
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Myeloid cell nuclear differentiation antigen-like protein
Loss of activity and dephosphorylation of CDH5.
Receptor-type tyrosine-protein phosphatase beta
Can hydrolyze nucleotides, with about fourfold higher rates for adenine versus uridine and no strong preference for diphosphates or triphosphates.
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5
No effect on its ability to hydrolyze NAD.
Cell growth-regulating nucleolar protein
Leucine-rich single-pass membrane protein 1
Glutamine amidotransferase-like class 1 domain-containing protein 3, mitochondrial
Pleckstrin homology-like domain family A member 1
Transcription and mRNA export factor ENY2
Coiled-coil domain-containing protein 183
Arginine-glutamic acid dipeptide repeats protein
Muscle, skeletal receptor tyrosine-protein kinase
Does not affect ability to promote piRNA biosynthesis.
Glycerol-3-phosphate acyltransferase 2, mitochondrial
Gly-Pro-Asn (GPN)-loop; involved in dimer interface
Putative ATP-dependent RNA helicase TDRD12
Chromodomain-helicase-DNA-binding protein 1
Omega-N-methylarginine; by PRMT6; alternate
Spindle assembly abnormal protein 6 homolog
Tricarboxylate transport protein, mitochondrial
Meiotic nuclear division protein 1 homolog
Mas-related G-protein coupled receptor member F
Pre-B-cell leukemia transcription factor 2
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
RNA polymerase-associated protein RTF1 homolog
Mitochondrial import inner membrane translocase subunit Tim29
Abolished cleavage by bacterial effector protein SpeB, preventing pyroptosis.
Mitochondrial import receptor subunit TOM6 homolog
4-hydroxybenzoate polyprenyltransferase, mitochondrial
SURP and G-patch domain-containing protein 1
SANT and BTB domain regulator of class switch recombination
tRNA (uracil-5-)-methyltransferase homolog A
Low-density lipoprotein receptor-related protein 10
Ankyrin repeat and death domain-containing protein 1B
Protein phosphatase 1 regulatory subunit 1A
Prostate tumor-overexpressed gene 1 protein homolog
Mitogen-activated protein kinase kinase kinase 21
Phosphoserine; by autocatalysis and MAP4K1
Pyroglutamylated RF-amide peptide receptor
CPX chromosomal region candidate gene 1 protein homolog
Coiled-coil domain-containing protein 127
Abolishes cortactin-mediated endocytosis of KCNA2.
No effect on interaction with KCNA2, but decreases KCNA2 levels at the cell membrane.
DNA replication complex GINS protein SLD5
DNA replication complex GINS protein SLD5, N-terminally processed
N-acetylthreonine; in DNA replication complex GINS protein SLD5, N-terminally processed
Microtubule-associated protein RP/EB family member 2
Abolishes methylation, no effect on subcellular location.
N-acetylvaline; in SUMO-activating enzyme subunit 1, N-terminally processed
SUMO-activating enzyme subunit 1, N-terminally processed
Epithelial-stromal interaction protein 1
Exocyst complex component 3-like protein 4
Linker for activation of T-cells family member 2
Coiled-coil domain-containing protein 15
Abolishes calcium-binding. Does not alter subcellular localization and does not prevent inhibition of calcineurin activity.
Mothers against decapentaplegic homolog 5
All trans-polyprenyl-diphosphate synthase PDSS2
Complete loss of disulfide oxidase activity in vitro.
Loss of interaction with RNF4; when associated with S-1702; R-1736 and V-1737.
Loss of interaction with RNF4; when associated with T-1629 and S-1702.
Loss of interaction with RNF4; when associated with T-1629; R-1736 and V-1737.
Reduces the inhibitory effect of the atypical PHD domain.
Acetyl-coenzyme A synthetase 2-like, mitochondrial
Absence of sumoylation. Increased FGF4 activation. No effect on nuclear localization.
Hepatoma-derived growth factor-related protein 3
Major intrinsically disordered NOTCH2-binding receptor 1-like homolog
DnaJ homolog subfamily C member 30, mitochondrial
Tetratricopeptide repeat protein 19, mitochondrial
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Cell death-inducing p53-target protein 1
General transcription factor IIE subunit 1
Vacuolar protein sorting-associated protein 28 homolog
NF-kappa-B inhibitor-interacting Ras-like protein 2
Iroquois-class homeodomain protein IRX-1
High mobility group nucleosome-binding domain-containing protein 3
High affinity immunoglobulin alpha and immunoglobulin mu Fc receptor
Cilia- and flagella-associated protein 100
Guanine nucleotide-binding protein-like 3
Mislocalized to the nucleoplasm. No effect on MDM2-binding.
Plasma membrane calcium-transporting ATPase 4
tRNA N6-adenosine threonylcarbamoyltransferase
Heterogeneous nuclear ribonucleoproteins A2/B1
Early embryonic lethality in homozygous mice. Male subfertility in heterozygous mice due to impaired spermatogenesis.
Mice develop normally and were born at the expected Mendelian ratio. Homozygous mice however lose body weight by P14-P16 and are susceptible to fatal epileptic seizures. Cells are extremely sensitive to peroxide-induced cell death because the enzyme is inactivated: The enzyme undergoes overoxidation and irreversible inactivation in the presence of exceeding concentrations of its substrates. Unlike the wild-type enzyme, which can form a selenylamide in the absence of reducing equivalents, thereby preventing its irreversible overoxidation, the mutant fails to form such an intermediate during its catalytic cycle.
Phospholipid hydroperoxide glutathione peroxidase
Mediates head to head self-assembly of N-terminal ends
Cyclic AMP-responsive element-binding protein 3-like protein 2
Loss of S1P cleavage; when associated with A-427.
Loss of S1P cleavage; when associated with V-430.
Processed cyclic AMP-responsive element-binding protein 3-like protein 2
Inter alpha-trypsin inhibitor, heavy chain 4
Intraflagellar transport protein 57 homolog
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Cell growth regulator with RING finger domain protein 1
Unable to inhibit TORC1 signaling; when associated with A-419 and A-422.
Unable to inhibit TORC1 signaling; when associated with A-419 and A-426.
Unable to inhibit TORC1 signaling; when associated with A-422 and A-426.
NF-kappa-B inhibitor-interacting Ras-like protein 1
Polyunsaturated fatty acid lipoxygenase ALOX12
Abolishes internalization after cross-linking.
Trafficking protein particle complex subunit 3
Loss of channel activity. Functions as dominant negative mutation that impairs function of wild-type channel subunits. Causes impaired Purkinje cell dendrite growth and premature death of cerebellar Purkinje cells.
No effect on voltage-dependent channel opening or current amplitude, but decreased rate of inactivation during prolonged depolarization.
Potassium voltage-gated channel subfamily C member 3
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Phosphatidylinositol 3-kinase regulatory subunit gamma
Breast cancer type 2 susceptibility protein homolog
Nuclear export signal; masked by interaction with SEM1
Causes twisted hair shafts, broken hair follicles at sebaceous gland level and occasional rupture of the hair bulb or epidermal cyst-like changes.
CKLF-like MARVEL transmembrane domain-containing protein 7
Double-strand-break repair protein rad21 homolog
Gamma-aminobutyric acid receptor subunit rho-2
Abolished interaction with MAP1LC3B and subsequent pexophagy.
Does not affect interaction with MAP1LC3B.
Induces abnormal auditory brainstem responses indicative of neuronal dysfunction along the auditory pathway.
Epithelial discoidin domain-containing receptor 1
Transmembrane protease serine 4 catalytic chain
Zinc finger SWIM domain-containing protein 7
28S ribosomal protein S12, mitochondrial
Decreases surface expression in transfected pro-B cells and impairs interaction with FCER1G.
Increases surface expression in transfected pro-B cells and impairs interaction with FCER1G.
Insulin-like growth factor 2 mRNA-binding protein 3
Histone deacetylase complex subunit SAP18
Impairs interactions with RNPS1, ACIN1 and PNN; reduces ASAP and PSAP complex assemblies.
In temperature-sensitive FT20 cell line; defective activity.
Results in loss of primer extension catalytic activity but retains ability to bind to the primase complex.
Prevents cleavage by CASP8 and subsequent inactivation.
Leucine-rich repeat-containing protein 10
Zinc finger and BTB domain-containing protein 17
Conserved oligomeric Golgi complex subunit 1
Loss of autocatalytic cleavage; when associated with A-212; A-218 and A-225.
Loss of autocatalytic cleavage; when associated with A-212; A-218 and A-387.
Loss of autocatalytic cleavage; when associated with A-212; A-225 and A-387.
Loss of autocatalytic cleavage; when associated with A-218; A-225 and A-387.
Loss of kinase ativity. Knockin mice show embryonic lethality caused by endothelial cell necroptosis leading to cardiovascular defects. Mlkl deficiency rescues the cardiovascular phenotype of knockin mice, but causes perinatal lethality caused by induction of pyroptosis.
Abolishes RNA binding; when associated with L-63 and L-65.
Abolishes RNA binding; when associated with L-63 and L-68.
Abolishes RNA binding; when associated with L-65 and L-68.
Brain-enriched guanylate kinase-associated protein
Proline-, glutamic acid- and leucine-rich protein 1
UDP-glucose:glycoprotein glucosyltransferase 1
A disintegrin and metalloproteinase with thrombospondin motifs 4
Almost abolishes response to semaphorin.
Strongly reduced response to semaphorin.
Ankyrin repeat domain-containing protein 17
Abolishes AMPH and BIN1 binding. Reduces EPS15, SNAP91 and auxilin binding.
Abolishes AMPH, BIN1, DGKD, EPS15, EPN1, auxilin and SNAP91 binding.
No effect on DENND1A-,DENND1B- nor DENND1C-binding.
Reduces SNAP91, AMPH and BIN1 binding. Abolishes AMPH and SNAP91 binding; when associated with A-916. Abolishes EPN1 and EPS15 binding; when associated with A-905.
Reduces interaction with CD4 endocytosis signal motif; when associated with AP2S1 S-15.
Strongly reduces AMPH and SNAP91 binding. Abolishes DENND1B-binding; no effect on DENND1A-, nor DENND1C-binding. Abolishes AMPH and SNAP91 binding; when associated with A-837.
Strongly reduces AMPH, SNAP91 and BIN1 binding. Slightly reduces EPS15 and auxilin binding.
Strongly reduces AMPH, SNAP91, auxilin and BIN1 binding. Abolishes EPN1 and EPS15 binding; when associated with A-837.
Strongly reduces phosphatidylinositol binding.
Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-56.
Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-55 and Q-57.
Strongly reduces phosphatidylinositol binding. Abolishes phosphatidylinositol binding; when associated with Q-56 and Q-57.
Abolishes active hydrolase probe binding in serine hydrolase assays.
NACHT, LRR and PYD domains-containing protein 9B
Trinucleotide repeat-containing gene 6B protein
CAP-Gly domain-containing linker protein 1
Hydroxysteroid dehydrogenase-like protein 2
Loss of binding to RAB27A, remaining cytoplasmic.
Ectonucleoside triphosphate diphosphohydrolase 4
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
Mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51
Latent-transforming growth factor beta-binding protein 1
Loss of catalytic activity and localization to cytoplasmic vesicle.
Loss of localization to cytoplasmic vesicle.
Beta-crystallin B3, N-terminally processed
N-acetylalanine; in Beta-crystallin B3, N-terminally processed
Myeloid-associated differentiation marker
Centrosome and spindle pole associated protein 1
Probable inactive 1-aminocyclopropane-1-carboxylate synthase-like protein 2
Immunoglobulin superfamily DCC subclass member 4
Partial inhibition of BMP2-induced cytodifferentiation; when associated with E-170.
Partial inhibition of BMP2-induced cytodifferentiation; when associated with E-219.
Rescues the inhibitory effects of ASPN on BMP2-induced cytodifferentiation.
Disintegrin and metalloproteinase domain-containing protein 10
Loss of binding to AP2A1 and AP2A2. Decreased localization to the plasma membrane.
Mitochondrial translation release factor in rescue
FERM, ARHGEF and pleckstrin domain-containing protein 2
Increases guanyl-nucleotide exchange factor activity with RAC1; when associated with Q-733.
Increases guanyl-nucleotide exchange factor activity with RAC1; when associated with R-730.
28S ribosomal protein S27, mitochondrial
Carcinoembryonic antigen-related cell adhesion molecule 16
Does not affect the interaction with TECTA nor subcellular localization.
Reduces phosphorylation. Abolishes phosphorylation; when associated with F-350.
Reduces phosphorylation. Impairs podosome rosette formation. Abolishes phosphorylation; when associated with F-340.
Succinyl-CoA:3-ketoacid coenzyme A transferase 2B, mitochondrial
Succinyl-CoA:3-ketoacid-coenzyme A transferase
Abolishes binding of glycoprotein targets.
Abolishes binding of glycoprotein targets. Strongly reduced ubiquitination of glycoprotein targets.
ST]-E-Y-X(3)-F motif 1; required for efficient microtubule binding and stabilization
ST]-E-Y-X(3)-F motif 2; required for efficient microtubule binding and stabilization
ST]-E-Y-X(3)-F motif 3; required for efficient microtubule binding
ST]-E-Y-X(3)-F motif 4; required for efficient microtubule binding and stabilization
Ankyrin repeat and BTB/POZ domain-containing protein 2
Translation machinery-associated protein 7
In C47 mutant; does not affect ability to mediate inflammatory response and cell death; when associated with N-296.
In C47 mutant; does not affect ability to mediate inflammatory response; when associated with 313-N-N-314.
In C60 mutant; impaired cleavage and ability to generate caspase-1 subunits p10 and p20; abolished ability to process inflammatory cytokine interleukin-1 beta (IL1B) without affecting ability to induce programmed cell death.
In C71 mutant; abolished cleavage and ability to generate caspase-1 subunits; abolished ability to process inflammatory cytokine interleukin-1 beta (IL1B) and ability to induce programmed cell death; when associated with N-103 and 296-N--N-314.
In C71 mutant; abolished cleavage and ability to generate caspase-1 subunits; abolished ability to process inflammatory cytokine interleukin-1 beta (IL1B) and ability to induce programmed cell death; when associated with N-103 and N-122.
In C71 mutant; abolished cleavage and ability to generate caspase-1 subunits; abolished ability to process inflammatory cytokine interleukin-1 beta (IL1B) and ability to induce programmed cell death; when associated with N-122 and 296-N--N-314.
Mediates autoprocessing but are unable to mediate cleavage of Gasdermin-D (GSDMD).
Eukaryotic translation initiation factor 3 subunit A
Does not increase glutathione transferase activity and reduces cellular protection.
Tumor necrosis factor receptor superfamily member 11A
Decreases affinity for NADP. Changes enzyme activity, leading to the production of testosterone and concomitantly reducing the production of epi-testosterone.
No effect on affinity for androstenedione. Slight increase of catalytic activity.
No effect on enzyme activity. Decreases affinity for NADP.
Reduces enzyme activity and affinity for substrate. Alters the stereospecificity, so that androstenedione is converted to testosterone.
Acyl-coenzyme A synthetase ACSM1, mitochondrial
Abolishes trypsin inhibitor activity. No effect on sperm binding.
Fails to bind sperm. No effect on trypsin inhibitor activity.
No effect on trypsin inhibitor activity or sperm binding.
Severely impairs sperm binding. No effect on trypsin inhibitor activity.
Coiled-coil domain-containing protein 25
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Strongly reduced riboflavin transport; when associated with 391-A--A-394.
Strongly reduced riboflavin transport; when associated with A-275.
Loss of deubiquitinating activity; when associated with R-358.
Loss of deubiquitinating activity; when associated with S-194.
Beta/gamma crystallin domain-containing protein 3
EF-hand calcium-binding domain-containing protein 4B
Loss of activity; when associated with A-159.
Loss of activity; when associated with F-161.
Structural maintenance of chromosomes protein 5
Faster electrophoretic migration typical of a size reduction probably due to glycosylation loss.
Faster electrophoretic migration typical of a size reduction probably due to glycosylation loss. Abolishes proteolytic cleavage.
Ras association domain-containing protein 3
Coiled-coil domain-containing protein 157
Interleukin-1 receptor-associated kinase 1
FHF complex subunit HOOK interacting protein 1B
Beclin 1-associated autophagy-related key regulator
Probable global transcription activator SNF2L2
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
In LCH mutant; abolishes formation of the tetramer and deoxynucleoside triphosphate (dNTPase) activity; when associated with C-112 and H-143.
In LCH mutant; abolishes formation of the tetramer and deoxynucleoside triphosphate (dNTPase) activity; when associated with L-109 and C-112.
In LCH mutant; abolishes formation of the tetramer and deoxynucleoside triphosphate (dNTPase) activity; when associated with L-109 and H-143.
Increased ability to restrict LINE-1 retrotransposon activity.
Mimicks phosphorylation state, reduced ability to restrict LINE-1 retrotransposon activity.
Increased cytoplasmic vacuole size. Knockin mice develop albinism and lysosomal-storage disease.
26S proteasome non-ATPase regulatory subunit 13
Abolishes formation of disulfide bonds and ability to interact with TGFB1; when associated with A-219.
Abolishes formation of disulfide bonds and ability to interact with TGFB1; when associated with A-363.
Interchain (with C-? in TGFB1); in linked form
Transforming growth factor beta activator LRRC33
Loss of N-terminal lipidation. Fails to localize to the photoreceptor outer segment.
No significant effect on phosphorylation by FER.
Platelet endothelial cell adhesion molecule
Reduces tyrosine phosphorylation by FER by about 60%.
BAH and coiled-coil domain-containing protein 1
Double C2-like domain-containing protein gamma
Activity-dependent neuroprotector homeobox protein
Transmembrane and coiled-coil domain-containing protein 4
N-terminal EF-hand calcium-binding protein 3
Recombining binding protein suppressor of hairless
Thioredoxin domain-containing protein 15
Abolished interaction with SLC25A4/ANT1.
Mitochondrial import inner membrane translocase subunit TIM44
Ubiquitin carboxyl-terminal hydrolase 28
Histidine-rich carboxyl terminus protein 1
N-acetylglycine; in Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed
Tyrosine--tRNA ligase, cytoplasmic, N-terminally processed
Tyrosine-protein phosphatase non-receptor type 7
2-fold increase in basal phospholipase activity.
Acyl-coenzyme A thioesterase 10, mitochondrial
Coordinator of PRMT5 and differentiation stimulator
FYVE, RhoGEF and PH domain-containing protein 1
Ankyrin repeat domain-containing protein 49
Vacuolar protein sorting-associated protein 18 homolog
Mediator of RNA polymerase II transcription subunit 11
Thrombospondin type-1 domain-containing protein 7A
Thrombospondin type-1 domain-containing protein 7A, soluble form
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Complement C1r-B subcomponent heavy chain
Complement C1r-B subcomponent light chain
Histone-lysine N-methyltransferase SETMAR
Kinky tail mutant mice show kinky tail and white spot at the belly; additionally, homozygous mutants show microphthalmia and anterior segment dysgenesis including corneal opacity, severe iris bombe (forward-bowing iris) and very shallow or absent anterior chamber.
Transcription elongation factor 1 homolog
Abolishes transcriptional repression of target genes. Abolishes interaction with PER2.
Decreases affinity for FBXL3. Slightly increases affinity for PER2.
Decreases transcriptional repression of target genes. Decreases FBXL3 binding. Increases PER2 binding.
Lengthen circadian period. No effect on repressive activity. Increases protein stability.
Loss of interaction with MAP1LC3B and significant decrease in its autophagy-mediated degradation; when associated with A-494.
Loss of interaction with MAP1LC3B and significant decrease in its autophagy-mediated degradation; when associated with A-497.
Loss of ubiquitination. No loss of interaction with DDB1-CUL4A complex.
No effect on circadian period length and protein stability.
No effect on its interaction with MAP1LC3B and moderate decrease in its autophagy-mediated degradation; when associated with A-287.
No effect on its interaction with MAP1LC3B and moderate decrease in its autophagy-mediated degradation; when associated with A-290.
No effect on its interaction with MAP1LC3B and moderate decrease in its autophagy-mediated degradation; when associated with A-488.
No effect on its interaction with MAP1LC3B and moderate decrease in its autophagy-mediated degradation; when associated with A-491.
Phosphomimetic mutant that leads to destabilization of the protein and abolishes ability to bind PER2; when associated with D-280.
Phosphomimetic mutant that leads to destabilization of the protein and abolishes ability to bind PER2; when associated with D-71.
Phosphomimetic mutant that leads to stabilization of the protein; when associated with A-280.
Phosphomimetic mutant that leads to stabilization of the protein; when associated with A-71.
Reduced MAPK-catalyzed in vitro phosphorylation. No effect on inhibition of CLOCK-BMAL1-mediated transcriptional activity.
Reduced inhibition of CLOCK-BMAL1-mediated transcriptional activity.
Reduced interaction with MAP1LC3B and significant decrease in its autophagy-mediated degradation; when associated with A-273.
Reduced interaction with MAP1LC3B and significant decrease in its autophagy-mediated degradation; when associated with A-276.
Sensitive to FBXL3-ediated degradation but noz affected by expression of FBXL21.
Strongly reduces FBXL3 binding. Reduces PER2 binding.
Potassium voltage-gated channel subfamily E member 3
Does not affect interaction with TBP. Knockin mice are fertile.
Transcription initiation factor TFIID subunit 7-like
Biorientation of chromosomes in cell division protein 1-like 1
Apoptotic cell corpse-containing phagosomes fail to mature into acidic phagosomes. Loss of PIK3C3 and RAB5A recruitment to phagosomes.
Loss of interaction with DLG4; when associated with A-832 and A-834.
Loss of the effect on myogenic differentiation; when associated with A-832. Loss of interaction with DLG4; when associated with A-832 and A-833.
Loss of the effect on myogenic differentiation; when associated with A-834. Loss of interaction with DLG4; when associated with A-833 and A-834.
Abolishes retention in the endoplasmic reticulum so that the protein is secreted.
C-Jun-amino-terminal kinase-interacting protein 4
Loss of autophosphorylation and kinase activity.
Cation channel sperm-associated auxiliary subunit zeta
Neuronal PAS domain-containing protein 2
Significant decrease in DNA binding affinity resulting in a loss of the transcriptional activity.
von Willebrand factor C domain-containing protein 2-like
Lethal(2) giant larvae protein homolog 1
Deficient in binding to RAN-GTP and in GTP release activity.
28S ribosomal protein S21, mitochondrial
Enhanced transcriptional activation; Greatly enhanced transcriptional activation; when associated with A-15.
Enhanced transcriptional activation; Greatly enhanced transcriptional activation; when associated with A-68.
No effect on transcriptional activation.
Nuclear receptor subfamily 2 group C member 2
Some repression of transcriptional activation; Repressed transcriptional activity by about 10-fold; when associated with E-19.
Some repression of transcriptional activation; Repressed transcriptional activity by about 10-fold; when associated with E-68.
BMP/retinoic acid-inducible neural-specific protein 3
Submaxillary gland androgen-regulated protein 3A
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
Insulin-like growth factor-binding protein 7
Zinc finger and BTB domain-containing protein 32
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Cyclin-dependent kinase 2-interacting protein
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Cytoplasmic dynein 1 light intermediate chain 1
U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
WD repeat domain phosphoinositide-interacting protein 4
SKI family transcriptional corepressor 1
Leucine-rich repeat-containing protein 3B
Coenzyme Q-binding protein COQ10 homolog B, mitochondrial
GTP cyclohydrolase 1 feedback regulatory protein
Transcription elongation factor A protein-like 1
Glycoprotein endo-alpha-1,2-mannosidase-like protein
Rho guanine nucleotide exchange factor 6
Transient receptor potential cation channel subfamily V member 2
LysM and putative peptidoglycan-binding domain-containing protein 2
Glutamine amidotransferase-like class 1 domain-containing protein 1
Leucine-rich repeat-containing protein 59
Leucine-rich repeat-containing protein 59, N-terminally processed
N-acetylthreonine; in Leucine-rich repeat-containing protein 59, N-terminally processed
Probable RNA polymerase II nuclear localization protein SLC7A6OS
Abolishes magnesium and calcium-binding to the EF-hand 5 domain (EF5).
Homozygous mice are not viable. Embryos show early lethality along with fetal growth restriction and lack of visible blood vessels in yolk sacs.
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
Trafficking protein particle complex subunit 6B
Abolishes interaction with NXF1; when associated with E-29.
Abolishes interaction with NXF1; when associated with E-30.
Asymmetric dimethylarginine; alternate; by PRMT1; in vitro
Asymmetric dimethylarginine; by PRMT1; in vitro
Omega-N-methylarginine; alternate; by PRMT1; in vitro
FRAS1-related extracellular matrix protein 1
Increases surface expression in transfected pro-B cells and enhances interaction with FCER1G. Slightly increases surface expression and enhances interaction with FCER1G; when associated with 189-Y--L-198.
Reduces surface expression in transfected pro-B cells and enhances interaction with FCER1G. Slightly increases surface expression in transfected pro-B cells and enhances interaction with FCER1G; when associated with 177-S--R-182.
In mei4; Sterility in both sexes due to meiotic defects.
5'(3')-deoxyribonucleotidase, mitochondrial
Abolishes very-long-chain acyl-CoA synthetase activity.
Diminishes LCFA import and decreases nucleotide binding. No effect on localization to the cell membrane.
Long-chain fatty acid transport protein 1
AT-rich interactive domain-containing protein 2
Presenilins-associated rhomboid-like protein, mitochondrial
RING-type E3 ubiquitin-protein ligase PPIL2
Nonsense-mediated mRNA decay factor SMG5
Baculoviral IAP repeat-containing protein 1b
Zinc finger and BTB domain-containing protein 43
Platelet-derived growth factor receptor-like protein
UDP-N-acetylhexosamine pyrophosphorylase
Disintegrin and metalloproteinase domain-containing protein 23
Tyrosine-protein phosphatase non-receptor type 14
Doublecortin domain-containing protein 2
Dimethyladenosine transferase 2, mitochondrial
Complex I assembly factor TIMMDC1, mitochondrial
Abrogates Malassezia recognition; when associated with D-171.
Abrogates Malassezia recognition; when associated with Q-169.
Confers specificity for glucose/mannose-type carbohydrates
UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter
Reduces TRIM25-mediated ubiquitination in response to DNA damage. Reduces recruitment of BLM to replication forks and impairs the restart of stalled replication forks.
Inhibits interaction with BLNK. No effect on interactions with TLR4, GRB2 and CSK.
Inhibits interaction with BLNK. Slightly inhibits interaction with GRB2. No effect on interactions with TLR4 and CSK.
Inhibits interaction with TLR4. Decreases IL12B expression after TLR4 activation by lipopolysaccharide. Inhibits interaction with CSK. No effect on interactions with LYN, GRB2, and BLNK.
Inhibits interaction with TLR4. Inhibits IL12B expression after TLR4 activation by lipopolysaccharide. No effect on interaction with LYN.
SLP adapter and CSK-interacting membrane protein
Abolishes assembly of dimers into functional tetramers. Loss of enzyme activity. Forms oligomers with full, phosphate-independent activity; when associated with A-325. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and A-325.
Constitutive enzyme activity that is fully active also in the absence phosphate. Forms oligomers with full, phosphate-independent activity; when associated with K-391. Forms dimers with full, phosphate-independent activity; when associated with Q-316 and K-391.
Forms dimers with full, phosphate-independent activity; when associated with A-325 and K-391.
Glutaminase kidney isoform, mitochondrial 65 kDa chain
Glutaminase kidney isoform, mitochondrial 68 kDa chain
Impairs tetramerization and promotes formation of homodimers. Impairs activation by phosphate.
Increased enzyme activity in the absence of phosphate. No effect on stimulation of enzyme activity by phosphate.
Increased stimulation of enzyme activity by phosphate.
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Phosphoethanolamine/phosphocholine phosphatase
Suppression of tumorigenicity 18 protein
Formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis; when associated with A-60. Increased formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis; when associated with A-32 and A-60.
Formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis; when associated with A-76. Increased formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis; when associated with A-32 and A-76.
Increases protein decay. forms subsarcolemmal aggregates. Changes the subcellular localization and turnover of its direct, extrasarcomeric binding partners. Knockin mice develop age-dependent desmin-positive protein aggregation pathology, skeletal muscle weakness, dilated cardiomyopathy, as well as cardiac arrhythmias and conduction defects. Knockin mice exhibit altered axial myofibrillar lattice arrangement in fast- and slow-twitch muscle fibers, increased axial stiffness and stretch-induced vulnerability in soleus muscle fiber bundles, reduced Ca(2+)-sensitivity in heterozygous fiber bundles and increased Ca(2+)-sensitivity in homozygous fiber bundles.
Reduced phosphorylation and formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis. Almost complete loss of phosphorylation; when associated with A-7 and A-28. Increased formation of unusual long bridge-like intermediate filament structures between two daughter cells during cytokinesis; when associated with A-60 and A-76.
Reduced phosphorylation. Almost complete loss of phosphorylation; when associated with A-28 and A-32.
Reduced phosphorylation. Almost complete loss of phosphorylation; when associated with A-7 and A-32.
Sodium/potassium-transporting ATPase subunit alpha-3
Eukaryotic translation initiation factor 3 subunit I
Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
Postacrosomal sheath WW domain-binding protein
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
(Microbial infection) N-beta-linked (GlcNAc) arginine
Tumor necrosis factor receptor superfamily member 1A
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-552. Partial loss of enhancement of LPS-induced TNF production; when associated with A-552.
Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-555. Partial loss of enhancement of LPS-induced TNF production; when associated with A-555.
Loss of phosphorylation. Loss of LYN-binding. Slight decrease in GRB2-binding. No effect on VAV-binding. Partial loss of enhancement of LPS-induced TNF production.
Nucleophile; for deubiquitinase activity
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
Aryl-hydrocarbon-interacting protein-like 1
In after-hour (afh) mutant; lengthening in circadian periodicity.
In overtime (ovtm) mutant; loss of function, lengthening of the circadian periodiocity and Cry1 stabilization of protein levels.
Soluble calcium-activated nucleotidase 1
Coiled-coil domain-containing protein 92
Radical S-adenosyl methionine domain-containing protein 2
Protein-associating with the carboxyl-terminal domain of ezrin
Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)
Glycyl serine ester (Ser-Gly) (interchain with G-Cter in ubiquitin)
Phosphothreonine; by PKA, PKB/AKT1, PIM1 and PIM2
Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction in transcriptional repression; when associated with A-197.
Reduced sumoylation levels. No effect on DNA-binding and slight reduction of transcriptional repression. Abolishes sumoylation. No effect on DNA-binding but great reduction of transcriptional repression; when associated with A-10.
Slight reduction of transcriptional repression. Great reduction of transcriptional repression; when associated with A-199.
Phospholipid phosphatase-related protein type 1
LHFPL tetraspan subfamily member 3 protein
Tubulin polymerization-promoting protein
No effect on dimerization. No effect on inhibition of BMP signaling.
Reduces affinity for BMP2 and BMP4. Reduces inhibition of BMP2 signaling.
Reduces affinity for BMP2 and BMP4. Strongly reduces inhibition of BMP2 signaling.
Slightly reduces affinity for BMP2 and BMP4. No effect on inhibition of BMP2 signaling.
Strongly reduces affinity for BMP2 and BMP4. Reduces inhibition of BMP2 signaling.
Strongly reduces affinity for BMP2 and BMP4. Strongly reduces inhibition of BMP2 signaling.
Interferon regulatory factor 2-binding protein 2
Myelin-associated neurite-outgrowth inhibitor
Receptor-type tyrosine-protein phosphatase T
Mas-related G-protein coupled receptor member A3
Glycerophosphodiester phosphodiesterase domain-containing protein 5
Reduction in class switch recombination of the immunoglobulin heavy chain in B cells.
Probable proline--tRNA ligase, mitochondrial
Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain
Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chain
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6
Mediator of RNA polymerase II transcription subunit 15
SPRY domain-containing SOCS box protein 3
Knockin mice exhibit olfaction deficits, male infertility, ventriculomegaly, retinopathy and obesity.
Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.
In cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane.
tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like
Tumor necrosis factor ligand superfamily member 8
Abolishes formation of a disulfide-linked heterodimer with KCNK2.
Increases channel expression at the cell membrane, resulting in higher channel activity.
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
Cytochrome c oxidase assembly protein COX14
Uncharacterized protein C12orf71 homolog
Pleckstrin homology domain-containing family A member 1
In strain: BALB/c; requires 2 nucleotide substitutions.
Interchain (with C-60 in C8-gamma chain)
Neuronal acetylcholine receptor subunit alpha-4
Leukocyte-associated immunoglobulin-like receptor 1
No effect on homotrimer formation, but loss of higher-order oligomeric complexes; when associated with A-29. Does not affect heterooligomerization with C1QL4; when associated with A-29.
No effect on homotrimer formation, but loss of higher-order oligomeric complexes; when associated with A-33. Does not affect heterooligomerization with C1QL4; when associated with A-33.
Mediator of RNA polymerase II transcription subunit 29
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synaptonemal complex central element protein 2
Coiled-coil domain-containing protein 96
Voltage-dependent calcium channel gamma-4 subunit
FHF complex subunit HOOK interacting protein 1A
Hydroxyacylglutathione hydrolase, mitochondrial
cAMP-dependent protein kinase type I-beta regulatory subunit
Cell migration-inducing and hyaluronan-binding protein
Almost abolishes interaction with BIRC2; when associated with A-292.
Almost abolishes interaction with BIRC2; when associated with A-294.
Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog
CREB-regulated transcription coactivator 1
No effect on interaction with 14-3-3 proteins.
Up-regulates CREB transcription factor activity.
In strain: AKR/J, C3H and DBA/2; requires 2 nucleotide substitutions.
Probable cationic amino acid transporter
STE20-related kinase adapter protein alpha
Guanylate cyclase soluble subunit beta-1
Prevents accumulation in pericentric heterochromatin.
Golgi-associated olfactory signaling regulator
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; E-346; E-360; R-363 and R-374.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360 and R-363.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360 and R-374.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360; R-363 and R-374.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; R-363 and R-374.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-345; E-346; E-360; R-363 and R-374.
Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-342; R-345; E-346; E-360; R-363 and R-374.
Probable ATP-dependent RNA helicase DDX41
Heparan sulfate glucosamine 3-O-sulfotransferase 3B1
Bipartite nuclear localization signal (NLS1)
Nuclear receptor subfamily 4 group A member 2
Polypeptide N-acetylgalactosaminyltransferase 6
In H2; induces a strong reduction in interaction with SRF and subsequent ability to prevent transcription of cardiac-specific genes.
In H3; does not affect the interaction with SRF and keeps its ability to prevent transcription of cardiac-specific genes.
Eukaryotic translation initiation factor 4E transporter
Reduces GRIA1 recycling. Abolishes the increase of spine density and size, as well as reduces the increase of GRIA1 expression in surface associated with long-term potentiation.
Tumor necrosis factor receptor superfamily member 13C
Decreases phosphorylation at S-232 and S-300 but does not affect activity or substrate metabolism.
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Pleckstrin homology domain-containing family G member 2
Guanine nucleotide-binding protein G(i) subunit alpha-1
Hippocampal cholinergic neurostimulating peptide
N-acetylalanine; in peptide hippocampal cholinergic neurostimulating
Phosphatidylethanolamine-binding protein 1
Serine/threonine-protein kinase MRCK alpha
Inactive carboxypeptidase-like protein X2
Dual specificity mitogen-activated protein kinase kinase 2
AT-rich interactive domain-containing protein 3A
Signal recognition particle 54 kDa protein
Guanine nucleotide-binding protein subunit beta-5
In Va and Va-J; constitutive active cation channel localized to plasma membrane.
Cytochrome c1, heme protein, mitochondrial
Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
ADP-ribosylation factor-like protein 6-interacting protein 4
Bone morphogenetic protein receptor type-2
receptor protein serine/threonine kinase
Nuclear factor erythroid 2-related factor 3
2-fold decrease in substrate affinity and 96% reduction in O-GlcNAcase activity. Regains appreciable level of catalytic efficiency in the acidic pH range. 40% recovery of activity in the presence of sodium azide.
2-fold increase in substrate affinity and 77% reduction in O-GlcNAcase activity. Regains appreciable level of catalytic efficiency in the acidic pH range. No recovery of activity in the presence of sodium azide.
3-fold increase in substrate affinity and 90% reduction in O-GlcNAcase activity. Regains appreciable level of catalytic efficiency in the acidic pH range. 70% recovery of activity in the presence of sodium azide.
No change in substrate affinity but 60% reduction in O-GlcNAcase activity.
DNA-directed RNA polymerase III subunit RPC3
Trichoplein keratin filament-binding protein
Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
ATP-binding cassette sub-family G member 3
Tyrosine-protein phosphatase non-receptor type substrate 1
Isocitrate dehydrogenase [NADP], mitochondrial
Ras association domain-containing protein 2
Loss of interaction with PPP1CA but no effect on interaction with TOX4 or WDR82. Cell cycle arrest at mitosis and cell death. Exhibits normal association with chromosomes but shows defects in the process of chromosome decondensation at late telophase.
Serine/threonine-protein phosphatase 1 regulatory subunit 10
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
Zinc finger and BTB domain-containing protein 5
RNA polymerase II elongation factor ELL3
Tubulin-specific chaperone cofactor E-like protein
Cilia- and flagella-associated protein 107
Abolished alkylation in knockin mice. Mice display elevated susceptibility to S.typhimurium infection.
40% decrease in IL1-induced NF-kappa-B activation.
Abolishes linear polyubiquitin-binding, impairs 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-293 and A-301.
Abolishes linear polyubiquitin-binding, impairs 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-293 and A-302.
Abolishes linear polyubiquitin-binding, impairs 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-301 and A-302.
Abolishes linear polyubiquitin-binding, impairs 'Lys-63'-linked polyubiquitin-binding and impairs of NF-kappa-B activation.
Abolishes linear polyubiquitin-binding, no effect on 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-309 and A-313.
Abolishes linear polyubiquitin-binding, no effect on 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-312 and A-313.
Abolishes linear polyubiquitin-binding; when associated with A-313 and A-317.
Abolishes linear polyubiquitin-binding; when associated with A-313 and A-320.
Decreases phosphorylation and increases NF-kappa-B activity.
Impairs linear polyubiquitin-binding. Abolishes linear polyubiquitin-binding, no effect on 'Lys-63'-linked polyubiquitin-binding and impairs NF-kappa-B activation; when associated with A-309 and A-312. Abolishes linear polyubiquitin-binding; when associated with A-317 and A-320.
Loss of interaction with TRAF6 and TRAF6-induced polyubiquitination.
Slight decrease in TRAF6-induced polyubiquitination.
Slight decrease in TRAF6-induced polyubiquitination. Decrease in TRAF6-induced polyubiquitination; when associated with R-318. Important decrease in TRAF6-induced polyubiquitination; when associated with R-314 and R-318.
Slight decrease in TRAF6-induced polyubiquitination. Decrease in TRAF6-induced polyubiquitination; when associated with R-319. Important decrease in TRAF6-induced polyubiquitination; when associated with R-314 and R-319.
Slight decrease in TRAF6-induced polyubiquitination. Important decrease in TRAF6-induced polyubiquitination; when associated with R-318 and R-319.
Geranylgeranyl transferase type-2 subunit alpha
Nucleolar and coiled-body phosphoprotein 1
Glycosylation-dependent cell adhesion molecule 1
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
Loss of phosphorylation but no loss of autophagic function; when associated with A-163.
Loss of phosphorylation but no loss of autophagic function; when associated with A-165.
Phosphatidylinositol 3-kinase catalytic subunit type 3
Beta-galactoside alpha-2,6-sialyltransferase 2
In L-5178-Y cell line; methotrexate-resistant, requires 2 nucleotide substitutions.
In a form with an abnormally low affinity for methotrexate.
Tumor protein p63-regulated gene 1-like protein
Affects the intracellular trafficking, resulting in endoplasmic reticulum retention. Mice carrying homozygous and heterozygous C509S exhibit a progressive decline in locomotor function and a reduction in the number of tyrosine hydroxylase-positive neurons.
Mitogen-activated protein kinase kinase kinase 4
Translocating chain-associated membrane protein 1-like 1
MAP kinase docking motif; essential for its phosphorylation
General transcription factor 3C polypeptide 2
Conserved oligomeric Golgi complex subunit 4
Zinc finger-containing ubiquitin peptidase 1
Instead of the formation of an intermediate complex with a thiol ester bond between ATG10 (E2-like enzyme) and ATG12 (substrate), a stable complex with an O-ester bond is formed. Does not affect ATG12 conjugation to ATG3.
Glutaredoxin domain-containing cysteine-rich protein 2
LYR motif 1; required for interaction with HSC20
LYR motif 2; not required for interaction with HSC20
Succinate dehydrogenase assembly factor 1, mitochondrial
Impairs interaction with CDC42 and RHOQ and reduces insulin-stimulated translocation to the plasma membrane.
Dystrophia myotonica WD repeat-containing protein
Loss of ubiquitin-protein ligase activity. Increase in TSG101-binding.
Leucine-rich repeat and fibronectin type-III domain-containing protein 3
Transcription initiation factor TFIID subunit 9B
Disrupts targeting to membrane; when associated with A-67; N-68; P-73; A-74 and Q-75.
Disrupts targeting to membrane; when associated with A-67; N-68; Q-70; A-74 and Q-75.
Disrupts targeting to membrane; when associated with A-67; N-68; Q-70; P-73 and A-74.
Disrupts targeting to membrane; when associated with A-67; N-68; Q-70; P-73 and Q-75.
Disrupts targeting to membrane; when associated with A-67; Q-70; P-73; A-74 and Q-75.
Disrupts targeting to membrane; when associated with N-68; Q-70; P-73; A-74 and Q-75.
Bardet-Biedl syndrome 10 protein homolog
Doublesex- and mab-3-related transcription factor 2
Nascent polypeptide-associated complex subunit alpha
Nascent polypeptide-associated complex subunit alpha, muscle-specific form
Mediator of RNA polymerase II transcription subunit 23
Saccharopine dehydrogenase-like oxidoreductase
Improved binding of dioxygen and carbon monoxide to the iron atom.
Mutant mice develop early onset ataxia, exhibit reduced body weight and usually die around 10 weeks after birth. Increases SPT affininity toward C18-CoA substrate by twofold and elevates C20 long chain base production in the brain and eye. Produces neurodegenerative effects such as aberrant membrane strucutres, accumulation of ubiquitinated protein on membranes and axon degeneration.
Serine palmitoyltransferase small subunit B
Conserved oligomeric Golgi complex subunit 2
Not detectable on the plasma membrane. Abolishes hyaluronan synthase activity.
Calcium-activated potassium channel subunit beta-4
Double-strand-break repair protein rad21-like protein 1
Peptidyl-prolyl cis-trans isomerase FKBP10
FYVE, RhoGEF and PH domain-containing protein 6
Melanocortin-2 receptor accessory protein 2
In allele Ren-1D; requires 2 nucleotide substitutions.
SH3 and cysteine-rich domain-containing protein 2
Long-chain specific acyl-CoA dehydrogenase, mitochondrial
Reduces activity by 23%; reduces activity by 80% when associated with R-318.
Reduces activity by 37%; reduces activity by 80% when associated with R-322.
Reduces activity by 90% when associated with R-318 and R-322.
39S ribosomal protein L11, mitochondrial
Abolished phosphorylation by MAPK1/MAPK3.
Strongly reduces interaction with RAB27A.
Strongly reduces interaction with STX1A.
Inactive histone-lysine N-methyltransferase 2E
Sodium- and chloride-dependent taurine transporter
Abolishes interaction with TYROBP or FcR gamma.
Abolishes interaction with DNM1, MAPT, SYNJ1 and WASL.
Abolishes membrane tubulation. No effect on phospholipid binding.
Abolishes membrane tubulation; when associated with E-127.
Abolishes membrane tubulation; when associated with E-130.
Protein kinase C and casein kinase substrate in neurons protein 1
Abolishes calcium uptake into mitochondria. Abolishes interaction with MCU.
DNA-directed RNA polymerase II subunit RPB4
ATP-binding cassette sub-family C member 9
Does not lead to glycosylation, suggesting that the non-cleavable signal sequence remains partly or entirely embedded in the membrane.
FXYD domain-containing ion transport regulator 3
Microtubule-actin cross-linking factor 1, isoforms 6/7
Almost complete loss of binding to G(o) alpha subunit. No inhibition of GTPase activity.
No MAPK8IP1 nor APBA1 nor APBB1 nor DAB1 binding.
No MAPK8IP1 nor APBA1 nor Dab1 binding. No effect on APBB1 binding.
Phosphothreonine; by CDK5 and MAPK10 and LRRK2
YENPXY motif; contains endocytosis signal
Solute carrier organic anion transporter family member 4C1
Glutamate-rich WD repeat-containing protein 1
Membrane-spanning 4-domains subfamily A member 6D
Abolished protease activity and ability to cleave OPA1. Abolished autocatalytic processing.
Metalloendopeptidase OMA1, mitochondrial
ATP-binding cassette sub-family D member 1
Histone-lysine N-methyltransferase SUV39H2
Biogenesis of lysosome-related organelles complex 1 subunit 3
Phosphoserine; by PKA, PKC, PKG/PRKG1 and ROCK1
Phosphothreonine; by PKA, PKG/PRKG1 and AMPK
Reduced binding to monomeric actin. No VASP-induced actin polymerization.
Reduces actin polymerization to a lesser extent than wild-type.
Reduces actin polymerization to a lesser extent than wild-type. Greater effect on actin polymerization; when associated with D-235 and E-274.
Constitutive active mutant. In a knockin model, mouse display increased amounts of head dips and open arm time on the maze, compared to littermate controls. In addition, they also explore further into the open arm on the elevated plus maze and are less active in the closed arm compared to littermate controls. Male knockin mice display no differences in anxiety, but their locomotor activity increases compared to non-transgenic littermates.
Impairs protein kinase activity and shuttling to the cytoplasm.
Mimicks phosphorylation state, leading to localization to the cytoplasm.
No p38-alpha/MAPK14-, p38-beta/MAPK11-, ERK3/MAPK6-, ERK4/MAPK4-induced activation.
Phosphothreonine; by MAPK11, MAPK14, MAPK4, MAPK6 and PKA
Pleckstrin homology domain-containing family M member 2
Leucine-rich repeat-containing protein 14B
Microtubule-associated proteins 1A/1B light chain 3A
Protein mono-ADP-ribosyltransferase PARP12
Solute carrier organic anion transporter family member 2B1
G-protein coupled receptor family C group 5 member B
WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
Loss of interaction with AXIN1. Loss of function in AXIN1 degradation. Loss of function in Wnt signaling.
Minor effect on binding and degradation of target proteins.
Strongly reduced binding and degradation of target proteins; when associated with D-158.
Strongly reduced binding of target proteins. Strongly reduced degradation of target proteins.
Abrogates nuclear localization activity.
Clear and exclusive nuclear accumulation.
Significant loss of transactivation activity.
Slight reduction in transactivation activity.
RING finger and transmembrane domain-containing protein 2
Alpha-2-macroglobulin receptor-associated protein
Coiled-coil domain-containing protein 8 homolog
PxLPxI/L motif; mediates interaction with ANKRA2
Displays constitutive phospholipid scramblase activity.
In 1DA mutant; abolished cleavage by caspase, preventing phospholipid scramblase activity.
Pro-neuregulin-4, membrane-bound isoform
Volume-regulated anion channel subunit LRRC8D
DNA-directed RNA polymerase III subunit RPC9
PiggyBac transposable element-derived protein 5
Fibronectin type 3 and ankyrin repeat domains 1 protein
In Tdrd9(KI); heterozygous and homozygous knockin male mice are infertile due to derepression of transposable elements. PiRNA biogenesis in not affected but piRNAs fail to accumulate in the nucleus.
Ubiquinol-cytochrome-c reductase complex assembly factor 1
Signal recognition particle receptor subunit beta
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8
Accumulates at the plasma membrane, increased half-life and increased CASP9-mediated cell death.
Serine/arginine repetitive matrix protein 1-like
Secretory carrier-associated membrane protein 2
Diminishes the number of microvilli-like structures.
No effect on microvilli-like structures.
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
Cadherin EGF LAG seven-pass G-type receptor 2
H-2 class I histocompatibility antigen, Q7 alpha chain
UV radiation resistance-associated protein
Polyadenylate-binding protein-interacting protein 2
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3
Mitochondrial import receptor subunit TOM34
Ras GTPase-activating-like protein IQGAP2
Abolishes ability to rescue Purkinje cell degeneration in pcd mice when expressed in a transgene.
Abolishes deglutamylase activity; when associated with Q-915.
Abolishes deglutamylase activity; when associated with S-912.
Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2 and SUMO3); alternate
Pyrin and HIN domain-containing protein 1
Transmembrane and coiled-coil domain protein 3
Cleavage and polyadenylation specificity factor subunit 6
Decreased levels of global dipeptidyl peptidase activity. Homozygous pups are born at the expected Mendelian rate and display no obvious morphological defects, but none survive to weaning. Homozygous pups display suckling defects and survive only when fed manually. The suckling defects are probably due to increased apoptosis of migratory tongue muscle progenitor cells, leading to defects in the development of intrinsic muscles in the distal tongue and decreased tongue size.
Post-GPI attachment to proteins factor 2
Potassium voltage-gated channel subfamily B member 1
Non-structural maintenance of chromosomes element 3 homolog
Molybdopterin synthase catalytic subunit
Ubiquitin carboxyl-terminal hydrolase 24
PWWP domain-containing DNA repair factor 3A
Loss of interaction with Ras. Loss of Ras activation. Loss of ubiquitination.
Partial loss of interaction with Ras. Partial loss of Ras activation. Partial loss of ubiquitination.
Ras-specific guanine nucleotide-releasing factor 2
Nitric oxide synthase-interacting protein
Abolishes interaction with the elongin BC complex.
Elongin BC and Polycomb repressive complex 2-associated protein
Decreases protein abundance. Strong decreases of cell surface localization. Strong decreases on alanine uptake activity.
No effect on protein abundance. Almost complete loss of cell surface localization. Decreases alanine uptake activity.
No effect on protein abundance. Increases localization to the cell membrane. Increases alanine uptake activity.
No effect on protein abundance. No effect on cell surface expression. Decreases alanine uptake activity.
No effect on protein abundance. No effect on cell surface localization. Decreases alanine uptake activity.
No effect on protein abundance. No effect on cell surface localization. Increases alanine uptake activity.
No effect on protein abundance. No effect on cell surface localization. No effect on alanine uptake activity.
No effect on protein abundance. Strong decrease of cell surface localization. Decreases alanine uptake.
No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake activity.
No effect on protein abundance. Strong decreases of cell surface localization. Decreases alanine uptake.
Sodium-dependent neutral amino acid transporter B(0)AT3
Transcription elongation factor A protein-like 9
Inactive serine/threonine-protein kinase VRK3
Probable E3 ubiquitin-protein ligase MID2
Platelet-derived growth factor subunit A
Killer cell lectin-like receptor subfamily I member 1
In Jll; dominant allele that increases enzyme activity, and decreases fasting insulin levels, fasting leptin levels, weight gain and fat accumulation when mice are kept on a high fat diet.
Cysteine sulfonic acid (-SO3H); alternate
Mitogen-activated protein kinase kinase kinase kinase 3
Sigma non-opioid intracellular receptor 1
No effect on recruitment to endocytic membrane. No effect on membrane fusion produced by sphingosine.
Loss of isomerase activity. No phenotype when introduced in mice, animals are viable and fertile with no microcephaly or defective cortical lamination; no splicing defect.
Peptidyl-prolyl cis-trans isomerase-like 1
Reduced tRNA dimethylallyltransferase activity.
Leucine-rich repeat and fibronectin type-III domain-containing protein 2
CBY1-interacting BAR domain-containing protein 1
Cytochrome c oxidase subunit 7B, mitochondrial
Ribosomal L1 domain-containing protein 1
Uncharacterized protein C21orf62 homolog
Leucine-rich repeat-containing protein 2
Ankyrin repeat and LEM domain-containing protein 2
FRAS1-related extracellular matrix protein 2
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
B-cell CLL/lymphoma 7 protein family member A
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
In strain: A.BY, B10.H7 and C3H.SW; correlated with B6dom1-negative phenotype.
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein
Galactoside alpha-(1,2)-fucosyltransferase 1
Succinate--hydroxymethylglutarate CoA-transferase
EF-hand calcium-binding domain-containing protein 7
Leucine-rich repeats and immunoglobulin-like domains protein 2
rRNA/tRNA 2'-O-methyltransferase fibrillarin-like protein 1
Abolished phosphorylation by PKA, leading to deacreased ability to activate transcription of target genes; does not affect subcellular localization; when associated with A-211.
Abolished phosphorylation by PKA, leading to deacreased ability to activate transcription of target genes; does not affect subcellular localization; when associated with A-64.
Intraflagellar transport protein 140 homolog
CCR4-NOT transcription complex subunit 2
Interferon alpha-inducible protein 27-like protein 2A
Loss of angiogenic activity. Increased ribonuclease activity.
Loss of angiogenic activity. No effect on ribonuclease activity.
Loss of ribonuclease activity. Loss of angiogenic activity.
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
Piezo-type mechanosensitive ion channel component 2
In strain: BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
In strain: BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, pgn2 and SWN/Msf.
In strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
In strain: C57BL/10SnJ, HMI/Msf, MSM/Msf and SWN/Msf.
Eukaryotic translation initiation factor 4E-binding protein 3
Vacuolar protein sorting-associated protein 37D
39S ribosomal protein L18, mitochondrial
NACHT, LRR and PYD domains-containing protein 4C
40% decrease in alanine uptake. Significantly decreases alanine transporter activity. Does not affect cell surface expression.
No significant effect on alanine uptake.
No significant effect on alanine uptake. Does not affect alanine transport activity.
Sodium-coupled neutral amino acid transporter 4
LIM and senescent cell antigen-like-containing domain protein 1
Mitochondrial-processing peptidase subunit alpha
Chromodomain-helicase-DNA-binding protein 6
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
116 kDa U5 small nuclear ribonucleoprotein component
Abolishes protection against axon degeneration.
ATP synthase F(0) complex subunit C1, mitochondrial
WD repeat and SOCS box-containing protein 2
Zinc finger CCCH domain-containing protein 4
DNA-directed RNA polymerase III subunit RPC4
Abolished ability to bind poly-ADP-ribose and inhibit PARP1.
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
Methionyl-tRNA formyltransferase, mitochondrial
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
Histidine protein methyltransferase 1 homolog
RNA polymerase I-specific transcription initiation factor RRN3
Retinoic acid early-inducible protein 1-delta
ETS domain-containing transcription factor ERF
Transcription factor TFIIIB component B'' homolog
Inositol 1,4,5-trisphosphate receptor type 3
In isoform ActR-IIB2 and isoform ActR-IIB4.
In isoform ActR-IIB3 and isoform ActR-IIB4.
Cleaved by subtilisin-like proprotein convertase 4.
Cleaved by subtilisin-like proprotein convertase.
Not cleaved by subtilisin-like proprotein convertase 4.
Not cleaved by subtilisin-like proprotein convertase 4. Not cleaved by subtilisin-like proprotein convertase; when associated with A-795.
Not cleaved by subtilisin-like proprotein convertase. Not cleaved by subtilisin-like proprotein convertase; when associated with A-1053.
Pleckstrin homology domain-containing family A member 2
Heterozygous mutant mice show anomalies of the kidney and urinary tract.
Nuclear envelope integral membrane protein 1
Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-376; A/E-380 and A/E-419.
Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-376; A/E-419 and A/E-420.
Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-366; A/E-380; A/E-419 and A/E-420.
Partial loss of phosphorylation and loss of interaction with RAN-GTP; when associated with A/E-376; A/E-380; A/E-419 and A/E-420.
Partial loss of phosphorylation and loss of interaction with RAN; when associated with A/E-366; A/E-376; A/E-380 and A/E-420.
X-linked lymphocyte-regulated protein PM1
Insulin-like growth factor-binding protein 5
Increased PRKAR2A-binding; when associated with P-245.
Loss of PRKAR2A-binding; when associated with P-197.
Loss of PRKAR2A-binding; when associated with P-206.
Does not affect phosphorylation by RPS6KA2.
1-acylglycerol-3-phosphate O-acyltransferase ABHD5
Megakaryocyte-associated tyrosine-protein kinase
2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
Inhibits interaction with ZDHHC13 and ZDHHC17.
Abolished DNA-binding without affecting binding to poly-ADP-ribose chains; when associated with A-254.
Abolished DNA-binding without affecting binding to poly-ADP-ribose chains; when associated with A-264.
Abolished binding to poly-ADP-ribose chains, preventing induction of parthanatos.
Apoptosis-inducing factor 1, mitochondrial
Increases catalytic efficiency 30-fold. Increases affinity for NADH 20-fold.
Increases redox potential, reacts faster to NADH and forms two-fold longer-lived CTC.
Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins); alternate
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
Abolished tyrosine carboxypeptidase activity.
No effect on tyrosine carboxypeptidase activity.
Reduced tyrosine carboxypeptidase activity.
Slightly reduced tyrosine carboxypeptidase activity.
Strongly reduced tyrosine carboxypeptidase activity.
Absence of auditory brain stem response (ABR) to click stimuli demonstrates that the mice are profoundly deaf. Distortion product otoacoustic emissions (DPOAEs) are absent at 4 weeks of age at all frequencies tested. Normal hair bundle morphology as at postnatal day (P) 5 the sensory epithelia are patterned into three rows of outer hair cells (OHCs) and one row of inner hair cells (IHCs). The bundles of OHCs appear similar in size to those of wild-type mice and form a normal staircase pattern.
In add; reduces methyltransferase activity; causes hyperkinesis, circling, head-tossing, aggression, progressive degeneration of the organ of Corti, hair cell defects and profound deafness. Distortion product otoacoustic emissions (DPOAEs) are absent at 4 weeks of age at all frequencies tested. At postnatal day (P) 5 the sensory epithelia are patterned into three rows of outer hair cells (OHCs) and one row of inner hair cells (IHCs) with no obvious structural abnormalities. The bundles of OHCs appear similar in size to those of wild-type mice and form a normal staircase pattern. The hair bundles of OHCs have a slightly more rounded morphology, especially in basal regions of the cochlea. The morphology of IHCs is not significantly altered. Hair cells are maintained in the presence of gentamicin, an aminoglycoside antibiotic that enters hair cells through their transduction channels and normally causes hair cell death. Mechanotransduction currents are very small in P4 hair cells and completely absent in P7 hair cells. Membrane potential, outward-evoked currents and nonlinear capacitance are normal in OHCs. No difference in the expression or localization of tip link proteins CDH23 and PCDH15 or ATP2B2, MYO7A, ESPN and WHRN proteins at P5-P8 in hair bundles of hair cells. No difference in the number of tip links in IHCs and OHCs at P7-P8. No difference in the levels of catecholamines, including norepinephrine, homovanillic acid (HVA) and norepinephrine, or serotonin levels in the inner ear. Loss of reverse polarity currents.
Is able to rescue the mechanotransduction defect of L-48.
Transmembrane O-methyltransferase homolog
Diminishes binding to phosphoinositides.
Activating transcription factor 7-interacting protein 1
Isochorismatase domain-containing protein 1
Abolishes interaction with APBA1. Abolishes transport of vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules.
Abolishes interaction with the FKBP1A-rapamycin complex.
Vasoactive intestinal polypeptide receptor 1
Ankyrin repeat domain-containing protein 53
Complement component receptor 1-like protein
Destabilization of TERC, impaired telomerase function, and reduced rRNA pseudouridylation and pre-rRNA processing.
H/ACA ribonucleoprotein complex subunit DKC1
Reduced rRNA pseudouridylation and pre-rRNA processing.
Cytosolic arginine sensor for mTORC1 subunit 1
Transmembrane emp24 domain-containing protein 6
Abolishes PKA-mediated phosphorylation. No enhanced calcium release. Abolishes PKA-mediated phosphorylation: When associated with A-990; A-1190; A-1351 and A-1581.
Inositol 1,4,5-trisphosphate receptor type 2
No effect on PKA-mediated phosphorylation. Abolishes PKA-mediated phosphorylation: When associated with A-937; A-1190; A-1351 and A-1581.
No effect on PKA-mediated phosphorylation. Abolishes PKA-mediated phosphorylation: When associated with A-937; A-990; A-1190 and A-1351.
No effect on PKA-mediated phosphorylation. Abolishes PKA-mediated phosphorylation: When associated with A-937; A-990; A-1190 and A-1581.
No effect on PKA-mediated phosphorylation. Abolishes PKA-mediated phosphorylation: When associated with A-937; A-990; A-1351 and A-1581.
No effect on PKA-mediated phosphorylation. Enhanced calcium release on PKA activation.
WD repeat and SOCS box-containing protein 1
Coiled-coil domain-containing protein 28B
Probable constitutively active mutant unable to hydrolyze GTP; increases degradation of the transferrin receptor.
Major facilitator superfamily domain-containing protein 1
Mislocalization to the plasma membrane. Localizes to lysosomes when expressed with wild-type Glmp.
No change in molecular weight, indicating lack of N-glycosylation.
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
Loss of catalytic activity; when associated with S-217 and A-378. Abolishes binding to UDP-GlcNAc; when associated with S-217.
Loss of catalytic activity; when associated with S-217 and A-401. Reduces the affinity for UDP-GlcNAc; when associated with S-217.
Protects from inactivation caused by air oxidation or thiol-reactive agents. Reduces the affinity for UDP-GlcNAc; when associated with A-378. Abolishes binding to UDP-GlcNAc; when associated with A-401. Loss of catalytic activity; when associated with A-378 and A-401.
Phosphatidylinositol-glycan biosynthesis class F protein
Required for excision 1-B domain-containing protein
Coiled-coil domain-containing protein 80
Putative phospholipase B-like 2 15 kDa form
Putative phospholipase B-like 2 28 kDa form
Putative phospholipase B-like 2 40 kDa form
Mesoderm induction early response protein 1
IQCJ-SCHIP1 readthrough transcript protein
Strain C57BL/6. With 1 methionine sulfoxide.
Inhibits endocytosis. Abolishes interaction with AP2A1.
M-phase-specific PLK1-interacting protein
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Transcription termination factor 1b, mitochondrial
Complex I assembly factor ACAD9, mitochondrial
Complete loss of kinase activity and of nuclear localization.
Complete loss of kinase activity and of nuclear localization. Complete loss of kinase activity and of nuclear localization; when associated with A-270 and A-272.
Loss of kinase activity and of autophosphorylation; loss of phosphorylation by CDK7; no effect on nuclear subcellular location.
Loss of kinase activity and of autophosphorylation; no effect on phosphorylation by CDK7.
Loss of kinase activity; loss of phosphorylation by CDK7; no effect on autophosphorylation.
Loss of kinase activity; no effect on nuclear subcellular location.
Partial loss of autocatalytic kinase activity. Complete loss of kinase activity and of nuclear localization; when associated with A-271 and A-272.
Partial loss of kinase activity. Complete loss of kinase activity and of nuclear localization; when associated with A-270 and A-272.
Causes a mild reduction of protein solubility.
Processed zona pellucida sperm-binding protein 3
Reduces protein solubility and causes formation of an abnormal disulfide bond between C-111 and C-139 in approximately half of the molecules.
85/88 kDa calcium-independent phospholipase A2
Leucine-rich repeat-containing protein 63
Reduced trypsin-binding; when associated with A-550.
Reduced trypsin-binding; when associated with A-573.
Attenuates light-induced phase shifts of locomotion and expression of c-Fos and mPer1 in the SCN.
Cyclic AMP-responsive element-binding protein 1
Loss of phosphorylation by RPS6KA4 and RPS6KA5.
Phosphoserine; by CaMK1, CaMK2, CaMK4, PKB/AKT1 or PKB/AKT2, RPS6KA3, RPS6KA4, RPS6KA5 and SGK1
Disrupts homotrimerization; disrupts interaction with SYNE2; when associated with A-432.
Disrupts homotrimerization; disrupts interaction with SYNE2; when associated with A-435.
Stabilizes homotrimerization; no effect on interaction with SYNE2.
Outer dynein arm-docking complex subunit 3
Endoplasmic reticulum resident protein 27
Translation initiation factor eIF-2B subunit alpha
Upstream-binding factor 1-like protein 1
Does not strongly affect N-terminal glutamine amidohydrolase activity.
Impaired N-terminal glutamine amidohydrolase activity.
Loss of N-terminal glutamine amidohydrolase activity.
Protein N-terminal glutamine amidohydrolase
Arginine-hydroxylase NDUFAF5, mitochondrial
Low-density lipoprotein receptor-related protein 12
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2
Carnitine O-palmitoyltransferase 1, muscle isoform
Neurogenic locus notch homolog protein 2
No effect on O-glycosylation by POGLUT1. Loss of O-xylosylation.
G-patch domain and KOW motifs-containing protein
Echinoderm microtubule-associated protein-like 3
Decreased alpha-tubulin alpha-elongation step of polyglutamylase activity.
Increased alpha-tubulin initiation step of polyglutamylase activity. Increased alpha-tubulin initiation step of polyglutamylase activity; when associated with I-362. Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179 and I-362. Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179, I-182, I-362 and H-367.
Loss of alpha-tubulin alpha-elongation polyglutamylase activity.
Loss of alpha-tubulin alpha-elongation step of polyglutamylase activity.
Small increase in alpha-tubulin initiation step of polyglutamylase activity. Increased alpha-tubulin initiation step of polyglutamylase activity; when associated with R-180. Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179 and R-180. Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179, R-180, I-362 and H-367.
Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179, R-180, I-182 and I-262.
Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with A-179, R-180, I-362 and H-367.
Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with R-180 and I-362. Strong increase in alpha-tubulin initiation step of polyglutamylase activity; when associated with R-180, I-182, I-362 and H-367.
Barrier-to-autointegration factor-like protein
Sterile alpha motif domain-containing protein 15
Dolichol phosphate-mannose biosynthesis regulatory protein
General transcription factor 3C polypeptide 5
Constitutive coactivator of PPAR-gamma-like protein 2
Probable cysteine--tRNA ligase, mitochondrial
Vacuolar protein sorting-associated protein 53 homolog
Ubiquitin-like modifier-activating enzyme 6
Lymphatic vessel endothelial hyaluronic acid receptor 1
Potassium voltage-gated channel subfamily KQT member 4
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Neuronal acetylcholine receptor subunit alpha-3
CKLF-like MARVEL transmembrane domain-containing protein 6
Drastic decrease of CBX5, CBX1 and CBX3 binding; When associated with A-991.
Drastic decrease of CBX5, CBX1 and CBX3 binding; When associated with A-993.
Paired immunoglobulin-like type 2 receptor beta
Hematopoietic progenitor cell antigen CD34
No effect on protein abundance. Mice homozygous for that mutation do not display any developmental abnormality.
Glycyl serine ester (Ser-Gly) (interchain with G-Cter in ubiquitin); alternate
H-2 class I histocompatibility antigen, D-B alpha chain
In K-less, no effect on ubiquitination; when associated with R-55, R-155, R-170, R-197, R-210, R-220, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-170, R-197, R-210, R-220 and R-267.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-170, R-197, R-210, R-220 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-170, R-197, R-210, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-170, R-197, R-220, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-170, R-210, R-220, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-155, R-197, R-210, R-220, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-55, R-92, R-170, R-197, R-210, R-220, R-267 and R-277.
In K-less, no effect on ubiquitination; when associated with R-92, R-155, R-170, R-197, R-210, R-220, R-267 and R-277.
In Ld KCST-less, strongly impairs ubiquitination; when associated with I-337; R-340; 350-A--A-356 and 360-R-R-361. In Ld tail 1S, restores ubiquitination; when associated I-337; R-340; 350-A--A-352; A-356 and 360-R-R-361.
In Ld KCST-less, strongly impairs ubiquitination; when associated with R-332; I-337 and 360-R-R-361.
In Ld KCST-less, strongly impairs ubiquitination; when associated with R-332; I-337; 350-A--A-356 and 360-R-R-361. In Ld tail 1S, restores ubiquitination; when associated R-332; I-337; 350-A--A-352; A-356 and 360-R-R-361.
In Ld KCST-less, strongly impairs ubiquitination; when associated with R-332; I-337; R-340 and 350-A--A-356. In Ld tail 1S, restores ubiquitination; when associated R-332; I-337; R-340; 350-A--A-352; A-356 and 360-R-R-361.
In Ld KCST-less, strongly impairs ubiquitination; when associated with R-332; R-340; 350-A--A-356 and 360-R-R-361. In Ld tail 1S, restores ubiquitination; when associated R-332; R-340; 350-A--A-352; A-356 and 360-R-R-361.
In Ld tail 1S, restores ubiquitination; when associated R-332; I-337; R-340 and A-356.
In Ld tail 1S, restores ubiquitination; when associated R-332; I-337; R-340; A-356 and 360-R-R-361.
FAST kinase domain-containing protein 3, mitochondrial
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
Zinc finger CCHC domain-containing protein 12
Chromodomain-helicase-DNA-binding protein 7
N(6)-adenine-specific methyltransferase METTL4
Proprotein convertase subtilisin/kexin type 5
Constitutively active in IL1B release. In conventional housing conditions, mice bearing this mutation develop a systemic inflammatory phenotype associated with elevated IL1B and IL18 levels at 3-5 months of age. In a germ-free environment, they exhibit neutrophilia and myocarditis.
In strain: CAST/EiJ, DBA/2J and PWK/PhJ.
NACHT, LRR and PYD domains-containing protein 1a
NACHT, LRR and PYD domains-containing protein 1a, C-terminus
NACHT, LRR and PYD domains-containing protein 1a, N-terminus
Loss of interaction with CALM1. No effect on desumoylase activity.
Significanly reduced interaction with SUMO2.
Significanly reduced interaction with SUMO2. Loss of desumoylase activity.
Probable helicase with zinc finger domain
Loss of function; when associated with A-268; A-320; A-322; A-400 and A-454.
Loss of function; when associated with A-268; A-320; A-322; A-400 and A-456.
Loss of function; when associated with A-268; A-320; A-322; A-454 and A-456.
Loss of function; when associated with A-268; A-320; A-400; A-454 and A-456.
Loss of function; when associated with A-268; A-322; A-400; A-454 and A-456.
Loss of function; when associated with A-320; A-322; A-400; A-454 and A-456.
Potassium voltage-gated channel subfamily H member 2
Outer mitochondrial transmembrane helix translocase