A multidimensional chromatography technology for in-depth phosphoproteome analysis.
Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.
Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations.
Sequencing and comparison of yeast species to identify genes and regulatory elements.
The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
The reference genome sequence of Saccharomyces cerevisiae: Then and now.
Completion of the Saccharomyces cerevisiae genome sequence allows identification of KTR5, KTR6 and KTR7 and definition of the nine-membered KRE2/MNT1 mannosyltransferase gene family in this organism.
Global analysis of protein expression in yeast.
The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Catalytic and DNA binding properties of the ogg1 protein of Saccharomyces cerevisiae: comparison between the wild type and the K241R and K241Q active-site mutant proteins.
Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine.
The Ogg1 protein of Saccharomyces cerevisiae: a 7,8-dihydro-8-oxoguanine DNA glycosylase/AP lyase whose lysine 241 is a critical residue for catalytic activity.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Analysis of a 26 kb region on the left arm of yeast chromosome XV.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA.
Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA.
Global analysis of protein localization in budding yeast.
Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Assimilation of endogenous nicotinamide riboside is essential for calorie restriction-mediated life span extension in Saccharomyces cerevisiae.
Isolation and characterization of a thiamin transport gene, THI10, from Saccharomyces cerevisiae.
Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae.
Phosphate responsive signaling pathway is a novel component of NAD+ metabolism in Saccharomyces cerevisiae.
Saccharomyces cerevisiae YOR071C encodes the high affinity nicotinamide riboside transporter Nrt1.
The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames.
Various cytosine/adenine permease homologues are involved in the toxicity of 5-fluorocytosine in Saccharomyces cerevisiae.
Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.
Complete DNA sequence of yeast chromosome II.
The GET complex mediates insertion of tail-anchored proteins into the ER membrane.
Yeast gene which suppresses the defect in protein export of a secY mutant of E. coli.
Lysine144 is essential for the catalytic activity of Saccharomyces cerevisiae transaldolase.
Molecular analysis of the structural gene for yeast transaldolase.
Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins.
The ILV5 gene of Saccharomyces cerevisiae is highly expressed.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Genome-wide analysis of yeast transcription upon calcium shortage.
Identification of the copper regulon in Saccharomyces cerevisiae by DNA microarrays.
Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae.
Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X.
Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae.
Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1.
Systematic discovery of new genes in the Saccharomyces cerevisiae genome.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Identification of a developmentally regulated septin and involvement of the septins in spore formation in Saccharomyces cerevisiae.
SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells.
The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI.
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS.
Isolation of yeast mutants defective in protein targeting to the vacuole.
Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae.
Multiple pathways for vacuolar sorting of yeast proteinase A.
Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases.
The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization.
The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene.
Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases.
Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination.
N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.
The DNA-damage signature in Saccharomyces cerevisiae is associated with single-strand breaks in DNA.
The enzymes with benzil reductase activity conserved from bacteria to mammals.
Purification and properties of threonine deaminase from Saccharomyces cerevisiae.
Regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae.
The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
The proteome of Saccharomyces cerevisiae mitochondria.
Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Structural and biochemical characterization of the yeast HD domain containing protein YGK1 reveals a metal-dependent nucleoside 5'-monophosphatase.
Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae.
The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae.
The yeast SPC22/23 homolog Spc3p is essential for signal peptidase activity.
Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation.
Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.
High-resolution yeast phenomics resolves different physiological features in the saline response.
Sro7p, a Saccharomyces cerevisiae counterpart of the tumor suppressor l(2)gl protein, is related to myosins in function.
Structurally conserved interaction of Lgl family with SNAREs is critical to their cellular function.
Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism.
The Saccharomyces cerevisiae SOP1 and SOP2 genes, which act in cation homeostasis, can be functionally substituted by the Drosophila lethal(2)giant larvae tumor suppressor gene.
The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p.
The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface.
Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis.
Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9.
A global topology map of the Saccharomyces cerevisiae membrane proteome.
A 12.8 kb segment, on the right arm of chromosome II from Saccharomyces cerevisiae including part of the DUR1,2 gene, contains five putative new genes.
Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.
The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane.
Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora protein kinase by redistributing Glc7.
Characterization of new proteins found by analysis of short open reading frames from the full yeast genome.
The complete DNA sequence of yeast chromosome III.
A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized morphogenesis signaling network in cell integrity.
Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.
The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair.
Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging.
A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase.
A role for the Pcl9-Pho85 cyclin-cdk complex at the M/G1 boundary in Saccharomyces cerevisiae.
Swi5 controls a novel wave of cyclin synthesis in late mitosis.
The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
New weakly expressed cell cycle-regulated genes in yeast.
The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes.
Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.
The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes catalyze a two-step reaction leading to a soluble LL-dityrosine-containing precursor of the yeast spore wall.
Functional analyses of PAU genes in Saccharomyces cerevisiae.
Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity.
Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast.
Sequence analysis of a 14.6 kb DNA fragment of Saccharomyces cerevisiae chromosome VII reveals SEC27, SSM1b, a putative S-adenosylmethionine-dependent enzyme and six new open reading frames.
Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic.
Yeast coatomer contains a subunit homologous to mammalian beta'-COP.
A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication.
Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen.
Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function.
PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae.
The Mcm2-7 complex has in vitro helicase activity.
A genetic screen for top3 suppressors in Saccharomyces cerevisiae identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA repair.
The yeast Shu complex couples error-free post-replication repair to homologous recombination.
Complete DNA sequence of yeast chromosome XI.
Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao
Analysis of phosphorylation of YJL084c, a yeast protein.
Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface.
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site.
Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.
Happy together: the life and times of Ty retrotransposons and their hosts.
Reverse transcriptase and integrase of the Saccharomyces cerevisiae Ty1 element.
Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence.
Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase.
The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae.
Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography.
Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.
The structure of the yeast mitochondrial ribosome.
Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae.
Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo.
Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast.
A new system for naming ribosomal proteins.
Crystal structure of the eukaryotic ribosome.
DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames.
Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.
Molecular basis of Diamond-Blackfan anemia: structure and function analysis of RPS19.
Specific role for yeast homologs of the Diamond Blackfan anemia-associated Rps19 protein in ribosome synthesis.
Structure and organization of two linked ribosomal protein genes in yeast.
The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.
The structure of the eukaryotic ribosome at 3.0 A resolution.
Enrichment-based proteogenomics identifies microproteins, missing proteins, and novel smORFs in Saccharomyces cerevisiae.
Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast.
Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii.
Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.
The late-annotated small ORF LSO1 is a target gene of the iron regulon of Saccharomyces cerevisiae.
Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition.
Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae.
Functional domains of a positive regulatory protein, PHO4, for transcriptional control of the phosphatase regulon in Saccharomyces cerevisiae.
Isolation, physical characterization and expression analysis of the Saccharomyces cerevisiae positive regulatory gene PHO4.
Mode of expression of the positive regulatory genes PHO2 and PHO4 of the phosphatase regulon in Saccharomyces cerevisiae.
Nine-amino-acid transactivation domain: establishment and prediction utilities.
Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85.
Regulation of PHO4 nuclear localization by the PHO80-PHO85 cyclin-CDK complex.
Roles of phosphorylation sites in regulating activity of the transcription factor Pho4.
The activation domain of a basic helix-loop-helix protein is masked by repressor interaction with domains distinct from that required for transcription regulation.
The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal.
The yeast regulatory gene PHO4 encodes a helix-loop-helix motif.
Absence of Dbp2p alters both nonsense-mediated mRNA decay and rRNA processing.
Autoregulation of expression of the yeast Dbp2p 'DEAD-box' protein is mediated by sequences in the conserved DBP2 intron.
Discovery of arginine methylation, phosphorylation, and their co-occurrence in condensate-associated proteins in Saccharomyces cerevisiae.
Expanding the yeast protein arginine methylome.
Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen.
RPC19, the gene for a subunit common to yeast RNA polymerases A (I) and C (III).
Sites of ubiquitin attachment in Saccharomyces cerevisiae.
The DEAD-box RNA helicase Dbp2 connects RNA quality control with repression of aberrant transcription.
The DEAD-box protein Dbp2 functions with the RNA-binding protein Yra1 to promote mRNP assembly.
The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames.
p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts.
MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae.
Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis.
Pga1 is an essential component of glycosylphosphatidylinositol-mannosyltransferase II of Saccharomyces cerevisiae.
The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, one of which encodes a protein similar to the human myotonic dystrophy kinase.
Biochemical and genetic characterization of a replication protein A dependent DNA helicase from the yeast, Saccharomyces cerevisiae.
DNA helicase III of Saccharomyces cerevisiae, encoded by YER176w (HEL1), highly unwinds covalently closed, circular DNA in the presence of a DNA topoisomerase and yRF-A.
Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.
Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency.
Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis.
Identification of Coq11, a new coenzyme Q biosynthetic protein in the CoQ-synthome in Saccharomyces cerevisiae.
Organization of mitochondrial gene expression in two distinct ribosome-containing assemblies.
Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics.
Genetic regulation mediated by thiamin pyrophosphate-binding motif in Saccharomyces cerevisiae.
A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.
A generic protein purification method for protein complex characterization and proteome exploration.
A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle.
Composition and functional characterization of the yeast spliceosomal penta-snRNP.
Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII.
Structure of the yeast mitochondrial large ribosomal subunit.
Molecular organization of target of rapamycin complex 2.
Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags.
Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae.
Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control.
Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance.
The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation.
Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae.
In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p.
Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II.
Yeast Isw1p forms two separable complexes in vivo.
Dim2p, a KH-domain protein required for small ribosomal subunit synthesis.
Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae.
Nob1p is required for cleavage of the 3' end of 18S rRNA.
PIN domain of Nob1p is required for D-site cleavage in 20S pre-rRNA.
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes.
Biochemical and structural studies of conserved Maf proteins revealed nucleotide pyrophosphatases with a preference for modified nucleotides.
DNA sequencing and analysis of 130 kb from yeast chromosome XV.
Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames.
The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames.
The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects.
The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p.
Expression of the DAL80 gene, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression.
The UGA43 negative regulatory gene of Saccharomyces cerevisiae contains both a GATA-1 type zinc finger and a putative leucine zipper.
A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase.
Dynamics and processivity of 40S ribosome scanning on mRNA in yeast.
Identification of five putative yeast RNA helicase genes.
A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames.
Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues.
NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes.
The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae.
The single amino acid changes in the yeast mitochondrial S4 ribosomal protein cause temperature-sensitive defect in the accumulation of mitochondrial 15S rRNA.
A single subunit, Dis3, is essentially responsible for yeast exosome core activity.
Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components.
Reconstitution, activities, and structure of the eukaryotic RNA exosome.
The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain.
The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities.
The yeast exosome and human PM-Scl are related complexes of 3'-->5' exonucleases.
Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity.
Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast.
Identification of Cox20p, a novel protein involved in the maturation and assembly of cytochrome oxidase subunit 2.
Mba1, a novel component of the mitochondrial protein export machinery of the yeast Saccharomyces cerevisiae.
Genetic control of recombination partner preference in yeast meiosis. Isolation and characterization of mutants elevated for meiotic unequal sister-chromatid recombination.
A conserved acidic motif is crucial for enzymatic activity of protein O-mannosyltransferases.
Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves.
O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae.
PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae.
Pmt1 mannosyl transferase is involved in cell wall incorporation of several proteins in Saccharomyces cerevisiae.
Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer.
Protein O-mannosyltransferases participate in ER protein quality control.
Structure-function analysis of the dolichyl phosphate-mannose: protein O-mannosyltransferase ScPmt1p.
The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital.
The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.
Transmembrane topology of pmt1p, a member of an evolutionarily conserved family of protein O-mannosyltransferases.
A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast.
Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae.
eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange.
Genome-wide transcriptional analysis in S. cerevisiae by mini-array membrane hybridization.
The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene.
Kar4p, a karyogamy-specific component of the yeast pheromone response pathway.
Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome III of Saccharomyces cerevisiae.
A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast.
Cell cycle-dependent assembly of a Gin4-septin complex.
Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function.
Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.
Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae.
Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast.
The septins: roles in cytokinesis and other processes.
Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis.
Manganese activation of superoxide dismutase 2 in Saccharomyces cerevisiae requires MTM1, a member of the mitochondrial carrier family.
Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene.
The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2.
Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae.
Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor.
A unified nomenclature for yeast autophagy-related genes.
Atg26 is not involved in autophagy-related pathways in Saccharomyces cerevisiae.
Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum.
Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion.
COPII subunit interactions in the assembly of the vesicle coat.
Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.
Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase.
SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation.
Sec16p potentiates the action of COPII proteins to bud transport vesicles.
Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae.
Selective packaging of cargo molecules into endoplasmic reticulum-derived COPII vesicles.
Yeast SEC16 gene encodes a multidomain vesicle coat protein that interacts with Sec23p.
A novel role of Mgm1p, a dynamin-related GTPase, in ATP synthase assembly and cristae formation/maintenance.
A novel two-step mechanism for removal of a mitochondrial signal sequence involves the mAAA complex and the putative rhomboid protease Pcp1.
Cells lacking Pcp1p/Ugo2p, a rhomboid-like protease required for Mgm1p processing, lose mtDNA and mitochondrial structure in a Dnm1p-dependent manner, but remain competent for mitochondrial fusion.
Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae.
Mitochondrial membrane remodelling regulated by a conserved rhomboid protease.
Processing of Mgm1 by the rhomboid-type protease Pcp1 is required for maintenance of mitochondrial morphology and of mitochondrial DNA.
Cloning and characterization of the first two genes of the non-oxidative part of the Saccharomyces cerevisiae pentose-phosphate pathway.
Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stress.
Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon.
DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae.
Predicting protein functions from redundancies in large-scale protein interaction networks.
Analysis of a 23 kb region on the left arm of yeast chromosome IV.
Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
Kei1: a novel subunit of inositolphosphorylceramide synthase, essential for its enzyme activity and Golgi localization.
Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth.
An integrated approach for finding overlooked genes in yeast.
A conserved phenylalanine of motif IV in superfamily 2 helicases is required for cooperative, ATP-dependent binding of RNA substrates in DEAD-box proteins.
A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA.
A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation.
A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase.
ATP- and ADP-dependent modulation of RNA unwinding and strand annealing activities by the DEAD-box protein DED1.
Analysis of the proteome of Saccharomyces cerevisiae for methylarginine.
Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase.
Ded1p, a conserved DExD/H-box translation factor, can promote yeast L-A virus negative-strand RNA synthesis in vitro.
Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region.
Requirement of the DEAD-Box protein ded1p for messenger RNA translation.
Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus.
The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity.
The p20 and Ded1 proteins have antagonistic roles in eIF4E-dependent translation in Saccharomyces cerevisiae.
Division of labor among the yeast Sol proteins implicated in tRNA nuclear export and carbohydrate metabolism.
Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family.
Yeast chromosome III: new gene functions.
Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase.
A homolog of mammalian, voltage-gated calcium channels mediates yeast pheromone-stimulated Ca2+ uptake and exacerbates the cdc1(Ts) growth defect.
A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast.
Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII.
Phosphorylation of proteins by inositol pyrophosphates.
Protein pyrophosphorylation by inositol pyrophosphates is a posttranslational event.
Atomic model for the membrane-embedded VO motor of a eukaryotic V-ATPase.
Expression of a proteolipid gene from a high-copy-number plasmid confers trifluoperazine resistance to Saccharomyces cerevisiae.
The 3.5-A cryoEM structure of nanodisc-reconstituted yeast vacuolar ATPase V0 proton channel.
VMA11 and VMA16 encode second and third proteolipid subunits of the Saccharomyces cerevisiae vacuolar membrane H+-ATPase.
VMA11, a novel gene that encodes a putative proteolipid, is indispensable for expression of yeast vacuolar membrane H(+)-ATPase activity.
Evidence for overlapping and distinct functions in protein transport of coat protein Sec24p family members.
Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.
Sfb2p, a yeast protein related to Sec24p, can function as a constituent of COPII coats required for vesicle budding from the endoplasmic reticulum.
The sequence of 12.8 kb from the left arm of chromosome XIV reveals a sigma element, a pro-tRNA and six complete open reading frames, one of which encodes a protein similar to the human leukotriene A4 hydrolase.
The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae.
The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic.
Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17.
Role of Tim21 in mitochondrial translocation contact sites.
Label-free measurement of the yeast short chain TAG lipase activity by ESI-MS after one-step esterification.
Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV.
The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant.
The TGL2 gene of Saccharomyces cerevisiae encodes an active acylglycerol lipase located in the mitochondria.
The mitochondrial intermembrane space-facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism.
Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.
The RET1 gene of yeast encodes the second-largest subunit of RNA polymerase III. Structural analysis of the wild-type and ret1-1 mutant alleles.
The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation.
Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progression.
High-confidence proteomic analysis of yeast lipid droplets identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation.
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome.
Multiple functions as lipase, steryl ester hydrolase, phospholipase, and acyltransferase of Tgl4p from the yeast Saccharomyces cerevisiae.
Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast.
Regulation of the yeast triacylglycerol lipases Tgl4p and Tgl5p by the presence/absence of nonpolar lipids.
Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae are localized to lipid particles.
The complete sequence of an 18,002 bp segment of Saccharomyces cerevisiae chromosome XI contains the HBS1, MRP-L20 and PRP16 genes, and six new open reading frames.
Comparative genotyping of the Saccharomyces cerevisiae laboratory strains S288C and CEN.PK113-7D using oligonucleotide microarrays.
Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth.
Disruption of 12 ORFs located on chromosomes IV, VII and XIV of Saccharomyces cerevisiae reveals two essential genes.
Mutational analyses of trimethylguanosine synthase (Tgs1) and Mud2: proteins implicated in pre-mRNA splicing.
Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm.
Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.
Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.
Dynamics of the Spb4 interactome monitored by affinity purification.
Dynamics of the putative RNA helicase Spb4 during ribosome assembly in Saccharomyces cerevisiae.
Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae.
Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI.
Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae.
Studies on the assembly characteristics of large subunit ribosomal proteins in S. cerevisae.
Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46.
Sequence analysis of a 31 kb DNA fragment from the right arm of Saccharomyces cerevisiae chromosome II.
Structure and expression of a yeast gene encoding the small heat-shock protein Hsp26.
hsp26 of Saccharomyces cerevisiae is related to the superfamily of small heat shock proteins but is without a demonstrable function.
High-resolution view of the yeast meiotic program revealed by ribosome profiling.
Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles.
The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast.
Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast.
Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex.
Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry.
New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing.
The RRM domain is dispensable for yeast U1-70K function.
The amino-terminal domain of yeast U1-70K is necessary and sufficient for function.
The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene.
A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation.
Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation.
Expanded lysine acetylation specificity of Gcn5 in native complexes.
Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7.
Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family.
Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex.
Isolation and characterization of TAF25, an essential yeast gene that encodes an RNA polymerase II-specific TATA-binding protein-associated factor.
Mapping histone fold TAFs within yeast TFIID.
Molecular architecture of the S. cerevisiae SAGA complex.
Molecular characterization of Saccharomyces cerevisiae TFIID.
Multi-protein complexes in eukaryotic gene transcription.
Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry.
The histone fold is a key structural motif of transcription factor TFIID.
The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway.
Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins.
Role of Nfu1 and Bol3 in iron-sulfur cluster transfer to mitochondrial clients.
Involvement of actin-related proteins in ATP-dependent chromatin remodeling.
A novel mitochondrial carrier protein Mme1 acts as a yeast mitochondrial magnesium exporter.
The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins.
A link between secretion and pre-mRNA processing defects in Saccharomyces cerevisiae and the identification of a novel splicing gene, RSE1.
Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly.
Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing.
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.
Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.
Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p.
Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating.
The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs.
The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae.
A proteomics approach to understanding protein ubiquitination.
A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
Cloning and molecular characterization of three genes, including two genes encoding serine hydroxymethyltransferases, whose inactivation is required to render yeast auxotrophic for glycine.
Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis.
The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine -- expression of the gene in Escherichia coli and purification and characterization of the enzyme.
Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae.
Far3 and five interacting proteins prevent premature recovery from pheromone arrest in the budding yeast Saccharomyces cerevisiae.
Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene.
A novel cross-phylum family of proteins comprises a KRR1 (YCL059c) gene which is essential for viability of Saccharomyces cerevisiae cells.
Functional and physical interactions of Krr1p, a Saccharomyces cerevisiae nucleolar protein.
The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae.
The small-subunit processome is a ribosome assembly intermediate.
Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus.
Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.
Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.
Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis.
Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex.
FR171456 is a specific inhibitor of mammalian NSDHL and yeast Erg26p.
Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis.
Regulation of ergosterol biosynthesis in Saccharomyces cerevisiae.
The effect of the erg26-1 mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae.
The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy.
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response.
A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.
Active genes are tri-methylated at K4 of histone H3.
Characterization of lysine 56 of histone H3 as an acetylation site in Saccharomyces cerevisiae.
DNA sequences of yeast H3 and H4 histone genes from two non-allelic gene sets encode identical H3 and H4 proteins.
Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase.
Dynamic competing histone H4 K5K8 acetylation and butyrylation are hallmarks of highly active gene promoters.
Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription.
Esa1p is an essential histone acetyltransferase required for cell cycle progression.
Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase.
Gene silencing: trans-histone regulatory pathway in chromatin.
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B.
Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms.
Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes.
Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I.
Identification and verification of lysine propionylation and butyrylation in yeast core histones using PTMap software.
Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification.
Insights into the role of histone H3 and histone H4 core modifiable residues in Saccharomyces cerevisiae.
Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.
Lysine succinylation and lysine malonylation in histones.
Lysine-79 of histone H3 is hypomethylated at silenced loci in yeast and mammalian cells: a potential mechanism for position-effect variegation.
Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II.
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
Molecular regulation of histone H3 trimethylation by COMPASS and the regulation of gene expression.
Organismal differences in post-translational modifications in histones H3 and H4.
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14.
Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae.
Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression.
Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.
Set2-catalyzed methylation of histone H3 represses basal expression of GAL4 in Saccharomyces cerevisiae.
Single-nucleosome mapping of histone modifications in S. cerevisiae.
Steady-state levels of histone acetylation in Saccharomyces cerevisiae.
Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions.
The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1.
The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4.
The Taf14 YEATS domain is a reader of histone crotonylation.
The occurrence of histone H3 and H4 in yeast.
The primary structure of yeast histone H3.
Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway.
Protein insertion into the mitochondrial inner membrane by a twin-pore translocase.
The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane.
Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane.
Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane.
Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel.
Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family.
Characterization of the SAC3 gene of Saccharomyces cerevisiae.
Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism.
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery.
The Saccharomyces cerevisiae LEP1/SAC3 gene is associated with leucine transport.
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery.
Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis.
A dominant negative mutation in Saccharomyces cerevisiae methionine aminopeptidase-1 affects catalysis and interferes with the function of methionine aminopeptidase-2.
Evidence that two zinc fingers in the methionine aminopeptidase from Saccharomyces cerevisiae are important for normal growth.
Molecular cloning, sequencing, deletion, and overexpression of a methionine aminopeptidase gene from Saccharomyces cerevisiae.
N-terminal methionine removal and methionine metabolism in Saccharomyces cerevisiae.
The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2.
The specificity in vivo of two distinct methionine aminopeptidases in Saccharomyces cerevisiae.
Yeast (Saccharomyces cerevisiae) methionine aminopeptidase I: rapid purification and improved activity assay.
Yeast methionine aminopeptidase I can utilize either Zn2+ or Co2+ as a cofactor: a case of mistaken identity?
Yeast methionine aminopeptidase type 1 is ribosome-associated and requires its N-terminal zinc finger domain for normal function in vivo.
Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase.
Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes.
Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae.
The complete sequence of K3B, a 7.9 kb fragment between PGK1 and CRY1 on chromosome III, reveals the presence of seven open reading frames.
Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition.
Yeast ubiquitin-like genes are involved in duplication of the microtubule organizing center.
Localization of proteins that are coordinately expressed with Cln2 during the cell cycle.
Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion.
Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8.
The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion.
Activity of phosphoforms and truncated versions of Ndt80, a checkpoint-regulated sporulation-specific transcription factor of Saccharomyces cerevisiae.
Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors.
Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2.
Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast.
Gametogenesis in yeast is regulated by a transcriptional cascade dependent on Ndt80.
NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae.
NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae.
Phosphorylation and maximal activity of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is dependent on Ime2.
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes.
Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80.
Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae.
Structure of the sporulation-specific transcription factor Ndt80 bound to DNA.
Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression.
The pachytene checkpoint prevents accumulation and phosphorylation of the meiosis-specific transcription factor Ndt80.
CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore.
Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p.
The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation.
Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames.
The action of N-terminal acetyltransferases on yeast ribosomal proteins.
Adenine phosphoribosyltransferase mutants in Saccharomyces cerevisiae.
Lethal accumulation of guanylic nucleotides in Saccharomyces cerevisiae HPT1-deregulated mutants.
Purification and characterization of hypoxanthine-guanine phosphoribosyltransferase from Saccharomyces cerevisiae.
Purification and characterization of the hypoxanthine-guanine phosphoribosyltransferase from Saccharomyces cerevisiae.
Studies of the kinetic mechanism of hypoxanthine-guanine phosphoribosyltransferase from yeast.
The isolation and characterization of Saccharomyces cerevisiae mutants that constitutively express purine biosynthetic genes.
Transcriptional regulation of the yeast gmp synthesis pathway by its end products.
The ankyrin repeat-containing protein Akr1p is required for the endocytosis of yeast pheromone receptors.
The complete sequence of a 9037 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII.
The histidine permease gene (HIP1) of Saccharomyces cerevisiae.
Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function.
Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export.
Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells.
Study of a region on yeast chromosome XIII that complements pet G199 mutants (COX7) and carries a new non-essential gene.
The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p.
Saccharomyces cerevisiae positive regulatory gene PET111 encodes a mitochondrial protein that is translated from an mRNA with a long 5' leader.
BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex.
BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo.
Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo.
New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability.
Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p.
Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates.
A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.
Definition of a spliceosome interaction domain in yeast Prp2 ATPase.
Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.
Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing.
Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.
Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing.
The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.
The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase.
The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA.
The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors.
Coa1 links the Mss51 post-translational function to Cox1 cofactor insertion in cytochrome c oxidase assembly.
Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly.
A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability.
Saccharomyces cerevisiae MHF complex structurally resembles the histones (H3-H4)(2) heterotetramer and functions as a heterotetramer.
Near-atomic resolution structural model of the yeast 26S proteasome.
Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes.
The 26S proteasome of the yeast Saccharomyces cerevisiae.
Aip1p interacts with cofilin to disassemble actin filaments.
Cofilin is an essential component of the yeast cortical cytoskeleton.
Cooperation of two actin-binding proteins, cofilin and Aip1, in Saccharomyces cerevisiae.
Identification of yeast cofilin residues specific for actin monomer and PIP2 binding.
Isolation of a yeast essential gene, COF1, that encodes a homologue of mammalian cofilin, a low-M(r) actin-binding and depolymerizing protein.
Structure determination of yeast cofilin.
Phosphorylation by casein kinase 2 regulates Nap1 localization and function.
Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes.
Targets of the cyclin-dependent kinase Cdk1.
Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
Molecular architecture of SMC proteins and the yeast cohesin complex.
Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication.
A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome.
Yeast GGA proteins interact with GTP-bound Arf and facilitate transport through the Golgi.
Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif.
Effects of mannoprotein mutations on Saccharomyces cerevisiae core oligosaccharide structure.
Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae.
Saccharomyces cerevisiae mannoprotein mutants. Isolation of the mnn5 mutant and comparison with the mnn3 strain.
Svp26 facilitates endoplasmic reticulum to Golgi transport of a set of mannosyltransferases in Saccharomyces cerevisiae.
Detection of the mycophenolate-inhibited form of IMP dehydrogenase in vivo.
Functional distinctions between IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast.
Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE.
A nuclear FK506-binding protein is a histone chaperone regulating rDNA silencing.
All cyclophilins and FK506 binding proteins are, individually and collectively, dispensable for viability in Saccharomyces cerevisiae.
Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein.
Proline isomerization of histone H3 regulates lysine methylation and gene expression.
Structure and activity of the peptidyl-prolyl isomerase domain from the histone chaperone Fpr4 toward histone H3 proline isomerization.
The FK506-binding protein, Fpr4, is an acidic histone chaperone.
A high resolution protein interaction map of the yeast Mediator complex.
A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II.
Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA.
Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme.
Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex.
Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts.
Mediator as a general transcription factor.
Preponderance of free mediator in the yeast Saccharomyces cerevisiae.
Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II.
Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction.
The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae.
The structural and functional organization of the yeast mediator complex.
Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis.
Blm10 binds to pre-activated proteasome core particles with open gate conformation.
Blm10 protein promotes proteasomal substrate turnover by an active gating mechanism.
Blm3 is part of nascent proteasomes and is involved in a late stage of nuclear proteasome assembly.
Expression of the expanded YFL007w ORF and assignment of the gene name BLM10.
Revisiting the yeast chromosome VI DNA sequence reveals a correction merging YFL007w and YFL006w to a single ORF.
Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening.
Structure of the Blm10-20 S proteasome complex by cryo-electron microscopy. Insights into the mechanism of activation of mature yeast proteasomes.
The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle.
The novel BLM3 gene encodes a protein that protects against lethal effects of oxidative damage.
Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses.
Molecular cloning and physical analysis of an 8.2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes.
Polymorphisms on the right arm of yeast chromosome III associated with Ty transposition and recombination events.
SRD1, a S. cerevisiae gene affecting pre-rRNA processing contains a C2/C2 zinc finger motif.
SRD1, a Saccharomyces cerevisiae suppressor of the temperature-sensitive pre-rRNA processing defect of rrp1-1.
A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex.
Genetic and molecular characterization of GAL83: its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiae.
Purification and characterization of Snf1 kinase complexes containing a defined beta subunit composition.
Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism.
The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex.
beta-subunits of Snf1 kinase are required for kinase function and substrate definition.
A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation.
A novel Rap1p-interacting factor, Rif2p, cooperates with Rif1p to regulate telomere length in Saccharomyces cerevisiae.
Action of a RAP1 carboxy-terminal silencing domain reveals an underlying competition between HMR and telomeres in yeast.
Counting of Rif1p and Rif2p on Saccharomyces cerevisiae telomeres regulates telomere length.
Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo.
Telomeric protein distributions and remodeling through the cell cycle in Saccharomyces cerevisiae.
The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae.
Yeast Ku protein plays a direct role in telomeric silencing and counteracts inhibition by rif proteins.
Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins.
Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.
Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.
Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation in Saccharomyces cerevisiae.
7The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex.
A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae.
A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.
Cap-binding protein 1-mediated and eukaryotic translation initiation factor 4E-mediated pioneer rounds of translation in yeast.
Genetic and physical interactions involving the yeast nuclear cap-binding complex.
Importin provides a link between nuclear protein import and U snRNA export.
Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3.
Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts.
Subunit architecture of multimeric complexes isolated directly from cells.
The interaction of the cap-binding complex (CBC) with eIF4G is dispensable for translation in yeast.
The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex.
Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins.
The open reading frame YAL048c affects the secretion of proteinase A in S. cerevisiae.
Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway.
A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis.
Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages.
The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis.
A proteolytic pathway that recognizes ubiquitin as a degradation signal.
Extragenic suppressors of mutations in the cytoplasmic C-terminus of SEC63 define five genes in Saccharomyces cerevisiae.
Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation.
RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit.
Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase.
Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast.
Son1p is a component of the 26S proteasome of the yeast Saccharomyces cerevisiae.
Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae.
The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo.
UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product.
A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase.
An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules.
RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex.
Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex.
Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase.
The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates.
GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p Galpha subunit and functions in a Ras-independent pathway.
Assembly of Tim9 and Tim10 into a functional chaperone.
Biogenesis of the protein import channel Tom40 of the mitochondrial outer membrane: intermembrane space components are involved in an early stage of the assembly pathway.
Distinct domains of small Tims involved in subunit interaction and substrate recognition.
Functional reconstitution of the import of the yeast ADP/ATP carrier mediated by the TIM10 complex.
Intermembrane space proteome of yeast mitochondria.
Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complex.
The Tim9p-Tim10p complex binds to the transmembrane domains of the ADP/ATP carrier.
The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex.
The role of Tim9p in the assembly of the TIM22 import complexes.
The structural basis of the TIM10 chaperone assembly.
Tim9, a new component of the TIM22.54 translocase in mitochondria.
Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins.
Transport of the ADP/ATP carrier of mitochondria from the TOM complex to the TIM22.54 complex.
Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria.
Novel E3 ubiquitin ligases that regulate histone protein levels in the budding yeast Saccharomyces cerevisiae.
A late form of nucleophagy in Saccharomyces cerevisiae.
A protein conjugation system essential for autophagy.
An interrelationship between autophagy and filamentous growth in budding yeast.
Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway.
Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways.
Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site.
Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy.
Crystallization of the Atg12-Atg5 conjugate bound to Atg16 by the free-interface diffusion method.
Expression, purification and crystallization of the Atg5-Atg16 complex essential for autophagy.
Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae.
Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.
Hierarchy of Atg proteins in pre-autophagosomal structure organization.
Impairing the bioenergetic status and the biogenesis of mitochondria triggers mitophagy in yeast.
In vivo reconstitution of autophagy in Saccharomyces cerevisiae.
Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae.
Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions.
Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation.
Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex.
Piecemeal microautophagy of the nucleus requires the core macroautophagy genes.
Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy.
Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae.
Structural and functional analyses of APG5, a gene involved in autophagy in yeast.
Structure of Atg5.Atg16, a complex essential for autophagy.
Structure of the Atg12-Atg5 conjugate reveals a platform for stimulating Atg8-PE conjugation.
Structure-function relationship of Atg12, a ubiquitin-like modifier essential for autophagy.
The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy.
The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation.
The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.
The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators.
Uth1p is involved in the autophagic degradation of mitochondria.
A large-scale full-length cDNA analysis to explore the budding yeast transcriptome.
Genome-wide identification of spliced introns using a tiling microarray.
Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII.
The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization.
ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3.
ADA5/SPT20 links the ADA and SPT genes, which are involved in yeast transcription.
DNA sequence analysis of a 13 kbp fragment of the left arm of yeast chromosome XV containing seven new open reading frames.
SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription.
SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae.
The ATM-related cofactor Tra1 is a component of the purified SAGA complex.
Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex.
A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae.
Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions.
A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae.
DNA sequence analysis of a 17 kb fragment of yeast chromosome XI physically localizes the MRB1 gene and reveals eight new open reading frames, including a homologue of the KIN1/KIN2 and SNF1 protein kinases.
Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum.
Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.
Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity.
Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus.
The components of the Saccharomyces cerevisiae mannosyltransferase complex M-Pol I have distinct functions in mannan synthesis.
Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae.
A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA.
A new yeast poly(A) polymerase complex involved in RNA quality control.
Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3' end formation of 5.8S rRNA in Saccharomyces cerevisiae.
SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.
Genetic identification of factors that modulate ribosomal DNA transcription in Saccharomyces cerevisiae.
A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization.
Assigning function to yeast proteins by integration of technologies.
Identification of a novel non-structural maintenance of chromosomes (SMC) component of the SMC5-SMC6 complex involved in DNA repair.
Characterization of the interaction between the J-protein Jac1p and the scaffold for Fe-S cluster biogenesis, Isu1p.
Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1p.
Iron-Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein-Protein Interaction.
J-domain protein, Jac1p, of yeast mitochondria required for iron homeostasis and activity of Fe-S cluster proteins.
Jac1, a mitochondrial J-type chaperone, is involved in the biogenesis of Fe/S clusters in Saccharomyces cerevisiae.
Sequence-specific interaction between mitochondrial Fe-S scaffold protein Isu and Hsp70 Ssq1 is essential for their in vivo function.
Ssq1, a mitochondrial Hsp70 involved in iron-sulfur (Fe/S) center biogenesis. Similarities to and differences from its bacterial counterpart.
Suppressors of superoxide dismutase (SOD1) deficiency in Saccharomyces cerevisiae. Identification of proteins predicted to mediate iron-sulfur cluster assembly.
The Hsp70 chaperone Ssq1p is dispensable for iron-sulfur cluster formation on the scaffold protein Isu1p.
The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation.
The mitochondrial proteins Ssq1 and Jac1 are required for the assembly of iron sulfur clusters in mitochondria.
Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase.
Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export.
Purification, functional reconstitution, and characterization of the Saccharomyces cerevisiae isoprenylcysteine carboxylmethyltransferase Ste14p.
The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins.
The Saccharomyces cerevisiae prenylcysteine carboxyl methyltransferase Ste14p is in the endoplasmic reticulum membrane.
Topological and mutational analysis of Saccharomyces cerevisiae Ste14p, founding member of the isoprenylcysteine carboxyl methyltransferase family.
Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI.
Yeast N-terminal amidase. A new enzyme and component of the N-end rule pathway.
GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization.
Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily.
The Saccharomyces cerevisiae TCM62 gene encodes a chaperone necessary for the assembly of the mitochondrial succinate dehydrogenase (complex II).
The chaperonin-related protein Tcm62p ensures mitochondrial gene expression under heat stress.
Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis.
Phosphorylation by Cdc28 activates the Cdc20-dependent activity of the anaphase-promoting complex.
Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.
TPR proteins as essential components of the yeast cell cycle.
A novel SET domain methyltransferase in yeast: Rkm2-dependent trimethylation of ribosomal protein L12ab at lysine 10.
Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab.
Dual targeting of Nfs1 and discovery of its novel processing enzyme, Icp55.
Global analysis of the mitochondrial N-proteome identifies a processing peptidase critical for protein stability.
Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae.
Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo.
Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates.
Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae.
Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae.
The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p.
The quaternary structure of DNA polymerase epsilon from Saccharomyces cerevisiae.
Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression.
The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical.
A protein interaction map for cell polarity development.
Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.
SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition.
SKG6, a suppressor gene of synthetic lethality of kex2Delta gas1Delta mutations, encodes a novel membrane protein showing polarized intracellular localization.
RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae.
Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.
Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures.
Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism.
Psi35 in the branch site recognition region of U2 small nuclear RNA is important for pre-mRNA splicing in Saccharomyces cerevisiae.
RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:{Psi}-synthase Pus7p.
Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine.
Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae.
The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs.
Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA.
U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP.
X-ray structures of U2 snRNA-branchpoint duplexes containing conserved pseudouridines.
Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p.
Identifying Pex21p as a protein that specifically interacts with yeast seryl-tRNA synthetase.
Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import.
Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA(Ser).
Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway.
Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII.
Yarrowia lipolytica Pex20p, Saccharomyces cerevisiae Pex18p/Pex21p and mammalian Pex5pL fulfil a common function in the early steps of the peroxisomal PTS2 import pathway.
Nucleotide sequence of the GDS1 gene of Saccharomyces cerevisiae.
ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae.
Analysis of congenital disorder of glycosylation-Id in a yeast model system shows diverse site-specific under-glycosylation of glycoproteins.
Pathogen-Related Yeast (PRY) proteins and members of the CAP superfamily are secreted sterol-binding proteins.
Role of the transcription activator Ste12p as a repressor of PRY3 expression.
Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae.
The undertranslated transcriptome reveals widespread translational silencing by alternative 5' transcript leaders.
Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins.
Stt4 PI 4-kinase localizes to the plasma membrane and functions in the Pkc1-mediated MAP kinase cascade.
Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX.
Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase.
Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.
A yeast phosphofructokinase insensitive to the Fructose 2,6-bisphosphate; allosteric activator. Glycolysis/metabolic regulation/allosteric control.
Activation by phosphate of yeast phosphofructokinase.
An electron microscopy study of the quarternary structure of yeast phosphofructokinase.
Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method.
Enolase takes part in a macromolecular complex associated to mitochondria in yeast.
Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast.
Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases.
Mutant studies of yeast phosphofructokinase.
Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae.
Physicochemical parameters and subunit composition of yeast phosphofructokinase.
Similarity of activation of yeast phosphofructokinase by AMP and fructose-2,6-bisphosphate.
Single point mutations in either gene encoding the subunits of the heterooctameric yeast phosphofructokinase abolish allosteric inhibition by ATP.
Stimulation of yeast phosphofructokinase activity by fructose 2,6-bisphosphate.
Studies on the function of yeast phosphofructokinase subunits by in vitro mutagenesis.
Temporal organization of the phosphofructokinase/fructose-1,6-biphosphatase cycle.
The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle.
The phosphofructokinase genes of yeast evolved from two duplication events.
GPX2, encoding a phospholipid hydroperoxide glutathione peroxidase homologue, codes for an atypical 2-Cys peroxiredoxin in Saccharomyces cerevisiae.
Genetic analysis of glutathione peroxidase in oxidative stress response of Saccharomyces cerevisiae.
Glutathione peroxidase 2 in Saccharomyces cerevisiae is distributed in mitochondria and involved in sporulation.
Saccharomyces cerevisiae expresses three phospholipid hydroperoxide glutathione peroxidases.
Finding functional features in Saccharomyces genomes by phylogenetic footprinting.
Disruption and functional analysis of six ORFs on chromosome IV: YDL053c, YDL072c, YDL073w, YDL076c, YDL077c and YDL080c.
Identification of peroxisomal acyl-CoA thioesterases in yeast and humans.
A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae.
Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication.
Analysis of a 70 kb region on the right arm of yeast chromosome II.
Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4.
Competing crossover pathways act during meiosis in Saccharomyces cerevisiae.
Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization.
Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease.
Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis.
Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage.
Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae.
Substrate specificity of the Saccharomyces cerevisiae Mus81-Mms4 endonuclease.
The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions.
The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast.
The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10.
Aquaporins in Saccharomyces: characterization of a second functional water channel protein.
Polymorphism of Saccharomyces cerevisiae aquaporins.
Gyp5p and Gyl1p are involved in the control of polarized exocytosis in budding yeast.
Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast.
Interaction of the Saccharomyces cerevisiae cortical actin patch protein Rvs167p with proteins involved in ER to Golgi vesicle trafficking.
Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs.
Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants.
Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV.
Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum.
Genetic analysis of Mps3 SUN domain mutants in Saccharomyces cerevisiae reveals an interaction with the SUN-like protein Slp1.
Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae.
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope.
Diverse RNA-binding proteins interact with functionally related sets of RNAs, suggesting an extensive regulatory system.
Identification of RNA recognition elements in the Saccharomyces cerevisiae transcriptome.
A histone fold TAF octamer within the yeast TFIID transcriptional coactivator.
Identification of stop codon readthrough genes in Saccharomyces cerevisiae.
A new gene involved in the transport-dependent metabolism of phosphatidylserine, PSTB2/PDR17, shares sequence similarity with the gene encoding the phosphatidylinositol/phosphatidylcholine transfer protein, SEC14.
Compartment-specific synthesis of phosphatidylethanolamine is required for normal heavy metal resistance.
Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth.
PDR16 and PDR17, two homologous genes of Saccharomyces cerevisiae, affect lipid biosynthesis and resistance to multiple drugs.
Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover.
The sequence of a 24,152 bp segment from the left arm of chromosome XIV from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.
Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs.
Phenotypes of yeast mutants lacking the mitochondrial protein Pet20p.
A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant.
A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis.
Chromosomal superkiller mutants of Saccharomyces cerevisiae.
Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system.
Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast.
Structure and nuclear localization signal of the SKI3 antiviral protein of Saccharomyces cerevisiae.
Structure-based assembly of protein complexes in yeast.
Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN.
Synthetic lethality of sep1 (xrn1) ski2 and sep1 (xrn1) ski3 mutants of Saccharomyces cerevisiae is independent of killer virus and suggests a general role for these genes in translation control.
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.
New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of Saccharomyces cerevisiae.
The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA.
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae.
A global protein kinase and phosphatase interaction network in yeast.
Global response of Saccharomyces cerevisiae to an alkylating agent.
The protein kinases of budding yeast: six score and more.
Definition of membrane topology and identification of residues important for transport in subunit a of the vacuolar ATPase.
Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase.
Evidence for a conserved 95-120 kDa subunit associated with and essential for activity of V-ATPases.
Function of the COOH-terminal domain of Vph1p in activity and assembly of the yeast V-ATPase.
Site-directed mutagenesis of the 100-kDa subunit (Vph1p) of the yeast vacuolar (H+)-ATPase.
Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors.
The VPH1 gene encodes a 95-kDa integral membrane polypeptide required for in vivo assembly and activity of the yeast vacuolar H(+)-ATPase.
Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase.
Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation.
A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery.
An ER-mitochondria tethering complex revealed by a synthetic biology screen.
Evolutionary conservation of biogenesis of beta-barrel membrane proteins.
Interaction between mitochondria and the actin cytoskeleton in budding yeast requires two integral mitochondrial outer membrane proteins, Mmm1p and Mdm10p.
Machinery for protein sorting and assembly in the mitochondrial outer membrane.
Maintenance of mitochondrial morphology is linked to maintenance of the mitochondrial genome in Saccharomyces cerevisiae.
Mitochondrial protein sorting: differentiation of beta-barrel assembly by Tom7-mediated segregation of Mdm10.
Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene.
Regulation of mitochondrial morphology and inheritance by Mdm10p, a protein of the mitochondrial outer membrane.
Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes.
Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region.
The mitochondrial morphology protein Mdm10 functions in assembly of the preprotein translocase of the outer membrane.
The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria.
Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p.
Characterization and mutational analysis of a cluster of three genes expressed preferentially during sporulation of Saccharomyces cerevisiae.
Increased copy number of the 5' end of the SPS2 gene inhibits sporulation of Saccharomyces cerevisiae.
Stimulation of later functions of the yeast meiotic protein kinase Ime2p by the IDS2 gene product.
The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae.
Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex.
Molecular architecture of a kinetochore-microtubule attachment site.
The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement.
The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends.
The mitotic spindle is required for loading of the DASH complex onto the kinetochore.
The yeast DASH complex forms closed rings on microtubules.
NAD+-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiae.
Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins.
GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae.
Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames.
An Nalpha-acetyltransferase responsible for acetylation of the N-terminal residues of histones H4 and H2A.
Characterization of the Saccharomyces cerevisiae cdc42-1ts allele and new temperature-conditional-lethal cdc42 alleles.
Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae.
Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae.
Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle.
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.
Effect of site-directed mutagenesis of conserved lysine residues upon Pas1 protein function in peroxisome biogenesis.
Functional association of the AAA complex and the peroxisomal importomer.
Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol.
Molecular snapshots of the Pex1/6 AAA+ complex in action.
PAS1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative ATPases.
Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p.
The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading.
Unique double-ring structure of the peroxisomal Pex1/Pex6 ATPase complex revealed by cryo-electron microscopy.
Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae.
Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast.
The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae.
The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes.
Yeast Rrp14p is a nucleolar protein involved in both ribosome biogenesis and cell polarity.
Yeast Rrp14p is required for ribosomal subunit synthesis and for correct positioning of the mitotic spindle during mitosis.
A requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation.
Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex.
Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization.
The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein.
Ubiquitin-specific proteases of Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of the UBP gene family.
Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation.
Cell surface polarization during yeast mating.
Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast.
Genome-wide responses to mitochondrial dysfunction.
Prm1 prevents contact-dependent lysis of yeast mating pairs.
Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling.
The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating.
The plasma membrane proteins Prm1 and Fig1 ascertain fidelity of membrane fusion during yeast mating.
Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p.
Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation.
Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation.
A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum.
A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly.
Ecm11 is located in the cell nucleus throughout mitosis.
A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I.
A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae.
The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast.
The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae.
Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria.
The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast.
A gated channel into the proteasome core particle.
Biogenesis, structure and function of the yeast 20S proteasome.
Crystal structure of the boronic acid-based proteasome inhibitor bortezomib in complex with the yeast 20S proteasome.
Crystal structures of salinosporamide A (NPI-0052) and B (NPI-0047) in complex with the 20S proteasome reveal important consequences of beta-lactone ring opening and a mechanism for irreversible binding.
PRE3, highly homologous to the human major histocompatibility complex-linked LMP2 (RING12) gene, codes for a yeast proteasome subunit necessary for the peptidylglutamyl-peptide hydrolyzing activity.
Structural basis for the activation of 20S proteasomes by 11S regulators.
Structure of 20S proteasome from yeast at 2.4-A resolution.
TMC-95-based inhibitor design provides evidence for the catalytic versatility of the proteasome.
A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy.
A yeast gene encoding a putative RNA helicase of the 'DEAD'-box family.
An essential role for the Saccharomyces cerevisiae DEAD-box helicase DHH1 in G1/S DNA-damage checkpoint recovery.
Crystal structure and functional analysis of DEAD-box protein Dhh1p.
Decapping and decay of messenger RNA occur in cytoplasmic processing bodies.
Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiae.
Functional conservation of Dhh1p, a cytoplasmic DExD/H-box protein present in large complexes.
Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway.
Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase.
Processing bodies require RNA for assembly and contain nontranslating mRNAs.
The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes.
The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1.
The yeast EDC1 mRNA undergoes deadenylation-independent decapping stimulated by Not2p, Not4p, and Not5p.
Binding of the Fkh1 forkhead associated domain to a phosphopeptide within the Mph1 DNA helicase regulates mating-type switching in budding yeast.
Biochemical basis of oxidative protein folding in the endoplasmic reticulum.
Ero1p oxidizes protein disulfide isomerase in a pathway for disulfide bond formation in the endoplasmic reticulum.
Ero1p: a novel and ubiquitous protein with an essential role in oxidative protein folding in the endoplasmic reticulum.
Modulation of cellular disulfide-bond formation and the ER redox environment by feedback regulation of Ero1.
Oxidative protein folding in eukaryotes: mechanisms and consequences.
Structure of Ero1p, source of disulfide bonds for oxidative protein folding in the cell.
The C-terminal domain of yeast Ero1p mediates membrane localization and is essential for function.
The ERO1 gene of yeast is required for oxidation of protein dithiols in the endoplasmic reticulum.
The FAD- and O(2)-dependent reaction cycle of Ero1-mediated oxidative protein folding in the endoplasmic reticulum.
Two pairs of conserved cysteines are required for the oxidative activity of Ero1p in protein disulfide bond formation in the endoplasmic reticulum.
A novel Hsp70 of the yeast ER lumen is required for the efficient translocation of a number of protein precursors.
Coordinated activation of Hsp70 chaperones.
Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation.
Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins.
Sequencing of a 9.2 kb telomeric fragment from the right arm of Saccharomyces cerevisiae chromosome XIV.
Analysis of a 22,956 bp region on the right arm of Saccharomyces cerevisiae chromosome XV.
Swt1, a novel yeast protein, functions in transcription.
Characterization of the MKS1 gene, a new negative regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae.
A role in vacuolar arginine transport for yeast Btn1p and for human CLN3, the protein defective in Batten disease.
Action of BTN1, the yeast orthologue of the gene mutated in Batten disease.
BTN1, a yeast gene corresponding to the human gene responsible for Batten's disease, is not essential for viability, mitochondrial function, or degradation of mitochondrial ATP synthase.
Interaction among Btn1p, Btn2p, and Ist2p reveals potential interplay among the vacuole, amino acid levels, and ion homeostasis in the yeast Saccharomyces cerevisiae.
The subcellular location of the yeast Saccharomyces homologue of the protein defective in the juvenile form of Batten disease.
The yeast model for Batten disease: mutations in BTN1, BTN2, and HSP30 alter pH homeostasis.
A La protein requirement for efficient pre-tRNA folding.
A complex pathway for 3' processing of the yeast U3 snoRNA.
A nuclear import pathway for a protein involved in tRNA maturation.
A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts.
A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase.
A yeast RNA binding protein that resembles the human autoantigen La.
Assembly of 5S ribosomal RNA is required at a specific step of the pre-rRNA processing pathway.
GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae.
Host factors that affect Ty3 retrotransposition in Saccharomyces cerevisiae.
Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs.
La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a yeast homolog of the La autoantigen is dispensable for growth.
Lsm proteins are required for normal processing of pre-tRNAs and their efficient association with La-homologous protein Lhp1p.
Multiple functional interactions between components of the Lsm2-Lsm8 complex, U6 snRNA, and the yeast La protein.
Nuclear import and the evolution of a multifunctional RNA-binding protein.
Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization.
Precursors to the U3 small nucleolar RNA lack small nucleolar RNP proteins but are stabilized by La binding.
The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability.
The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.
The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors.
Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes.
Ty3 requires yeast La homologous protein for wild-type frequencies of transposition.
U snRNP assembly in yeast involves the La protein.
NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae.
A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.
The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins.
A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.
A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins.
Ubp15p, a ubiquitin hydrolase associated with the peroxisomal export machinery.
An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes.
Analysis of beta-1,3-glucan assembly in Saccharomyces cerevisiae using a synthetic interaction network and altered sensitivity to caspofungin.
Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase.
Calcineurin-dependent growth of an FK506- and CsA-hypersensitive mutant of Saccharomyces cerevisiae.
Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae.
Differential expression and function of two homologous subunits of yeast 1,3-beta-D-glucan synthase.
Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae.
FKS1 mutations responsible for selective resistance of Saccharomyces cerevisiae to the novel 1,3-beta-glucan synthase inhibitor arborcandin C.
Identification of two cell cycle regulated genes affecting the beta 1,3-glucan content of cell walls in Saccharomyces cerevisiae.
Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase.
In vitro activity of 1,3-beta-D-glucan synthase requires the GTP-binding protein Rho1.
Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis.
Mutations in Fks1p affect the cell wall content of beta-1,3- and beta-1,6-glucan in Saccharomyces cerevisiae.
Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.
Prenylation of Saccharomyces cerevisiae Chs4p Affects Chitin Synthase III activity and chitin chain length.
The Saccharomyces cerevisiae FKS1 (ETG1) gene encodes an integral membrane protein which is a subunit of 1,3-beta-D-glucan synthase.
The yeast FKS1 gene encodes a novel membrane protein, mutations in which confer FK506 and cyclosporin A hypersensitivity and calcineurin-dependent growth.
Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.
PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.
Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases.
The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon.
The yeast gene YJR025c encodes a 3-hydroxyanthranilic acid dioxygenase and is involved in nicotinic acid biosynthesis.
Characterization of the ATPase and unwinding activities of the yeast DEAD-box protein Has1p and the analysis of the roles of the conserved motifs.
Exploration of essential gene functions via titratable promoter alleles.
Has1p, a member of the DEAD-box family, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae.
Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation.
Rrp15p, a novel component of pre-ribosomal particles required for 60S ribosome subunit maturation.
SET1, a yeast member of the Trithorax family, functions in transcriptional silencing and diverse cellular processes.
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
Differential regulation of FUS3 MAP kinase by tyrosine-specific phosphatases PTP2/PTP3 and dual-specificity phosphatase MSG5 in Saccharomyces cerevisiae.
Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases.
Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1.
Saccharomyces cerevisiae forms D-2-hydroxyglutarate and couples its degradation to D-lactate formation via a cytosolic transhydrogenase.
The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism.
A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating.
Bakers' yeast, a model for fungal biofilm formation.
Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae.
Differential Flo8p-dependent regulation of FLO1 and FLO11 for cell-cell and cell-substrate adherence of S.cerevisiae S288c.
Expression and characterization of the flocculin Flo11/Muc1, a Saccharomyces cerevisiae mannoprotein with homotypic properties of adhesion.
FLO11, a yeast gene related to the STA genes, encodes a novel cell surface flocculin.
Gene fusion is a possible mechanism underlying the evolution of STA1.
Interactions by the Fungal Flo11 Adhesin Depend on a Fibronectin Type III-like Adhesin Domain Girdled by Aromatic Bands.
Intragenic tandem repeats generate functional variability.
Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family.
Molecular Basis for Strain Variation in the Saccharomyces cerevisiae Adhesin Flo11p.
Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast.
Shedding of the mucin-like flocculin Flo11p reveals a new aspect of fungal adhesion regulation.
Similar short elements in the 5' regions of the STA2 and SGA genes from Saccharomyces cerevisiae.
The N-terminal domain of the Flo11 protein from Saccharomyces cerevisiae is an adhesin without mannose-binding activity.
AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae.
Crystal structure of Prp8 reveals active site cavity of the spliceosome.
Mechanism for Aar2p function as a U5 snRNP assembly factor.
The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4.
The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p.
Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae.
Parallel identification of new genes in Saccharomyces cerevisiae.
Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4.
Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae.
Parallel phenotypic analysis of sporulation and postgermination growth in Saccharomyces cerevisiae.
Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous deletion strains of Saccharomyces cerevisiae.
The transcriptional program of sporulation in budding yeast.
Molecular genetics of ICL2, encoding a non-functional isocitrate lyase in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae ICL2 gene encodes a mitochondrial 2-methylisocitrate lyase involved in propionyl-coenzyme A metabolism.
Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast.
Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.
VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation.
A ubiquitin mutant with specific defects in DNA repair and multiubiquitination.
A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain.
Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding.
Structural basis for the specificity of ubiquitin C-terminal hydrolases.
Structure of a conjugating enzyme-ubiquitin thiolester intermediate reveals a novel role for the ubiquitin tail.
The yeast ubiquitin gene: head-to-tail repeats encoding a polyubiquitin precursor protein.
The yeast ubiquitin genes: a family of natural gene fusions.
Ubiquitination of stalled ribosome triggers ribosome-associated quality control.
AAT1, a gene encoding a mitochondrial aspartate aminotransferase in Saccharomyces cerevisiae.
The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast.
Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex.
Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy.
Structural and functional insights into Saccharomyces cerevisiae Tpa1, a putative prolylhydroxylase influencing translation termination and transcription.
Structure of the ribosomal oxygenase OGFOD1 provides insights into the regio- and stereoselectivity of prolyl hydroxylases.
Tpa1p is part of an mRNP complex that influences translation termination, mRNA deadenylation, and mRNA turnover in Saccharomyces cerevisiae.
CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae.
Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides.
Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway.
Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit.
Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein.
Purification of protein A-tagged yeast ran reveals association with a novel karyopherin beta family member, Pdr6p.
The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance.
The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus.
A family of hexosephosphate mutases in Saccharomyces cerevisiae.
Genetic control of phosphoglucomutase variants in Saccharomyces cerevisiae.
Phosphoglucomutase. V. Multiple forms of phosphoglucomutase.
The PGM3 gene encodes the major phosphoribomutase in the yeast Saccharomyces cerevisiae.
The effect of mutation of two forms of phosphoglucomutase in Saccharomyces.
Dolichol biosynthesis in the yeast Saccharomyces cerevisiae: an insight into the regulatory role of farnesyl diphosphate synthase.
Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae.
Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae.
The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein.
Truncated forms of a novel yeast protein suppress the lethality of a G protein alpha subunit deficiency by interacting with the beta subunit.
A general strategy to uncover transcription factor properties identifies a new regulator of drug resistance in yeast.
CCR4, a 3'-5' poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase.
Ccr4p is the catalytic subunit of a Ccr4p/Pop2p/Notp mRNA deadenylase complex in Saccharomyces cerevisiae.
Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region.
Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex.
The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5.
The CCR4 protein from Saccharomyces cerevisiae contains a leucine-rich repeat region which is required for its control of ADH2 gene expression.
The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively.
The transcription factor associated Ccr4 and Caf1 proteins are components of the major cytoplasmic mRNA deadenylase in Saccharomyces cerevisiae.
The study of methionine uptake in Saccharomyces cerevisiae reveals a new family of amino acid permeases.
Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins.
Structural evidence for Scc4-dependent localization of cohesin loading.
The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions.
Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes.
Transcriptional regulation of the Saccharomyces cerevisiae DAL5 gene family and identification of the high affinity nicotinic acid permease TNA1 (YGR260w).
Components of a Fanconi-like pathway control Pso2-independent DNA interstrand crosslink repair in yeast.
Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.
Genome-wide requirements for resistance to functionally distinct DNA-damaging agents.
MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage.
Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates.
Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase.
The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing.
Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from homologous recombination, but not from postreplicative repair.
Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.
Genetic and molecular interactions of the Erv41p-Erv46p complex involved in transport between the endoplasmic reticulum and Golgi complex.
Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase.
PKR1 encodes an assembly factor for the yeast V-type ATPase.
Role of Vma21p in assembly and transport of the yeast vacuolar ATPase.
Vma21p is a yeast membrane protein retained in the endoplasmic reticulum by a di-lysine motif and is required for the assembly of the vacuolar H(+)-ATPase complex.
Vma9p (subunit e) is an integral membrane V0 subunit of the yeast V-ATPase.
Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum.
Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex.
Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family.
Characterization of two homologous yeast genes that encode mitochondrial iron transporters.
Mutations in Saccharomyces cerevisiae iron-sulfur cluster assembly genes and oxidative stress relevant to Cu,Zn superoxide dismutase.
Autocatalytic processing of the ATP-dependent PIM1 protease: crucial function of a pro-region for sorting to mitochondria.
Cleavage site selection within a folded substrate by the ATP-dependent lon protease.
Cryo-EM structure of hexameric yeast Lon protease (PIM1) highlights the importance of conserved structural elements.
Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1.
Mitochondrial Lon of Saccharomyces cerevisiae is a ring-shaped protease with seven flexible subunits.
Promotion of mitochondrial membrane complex assembly by a proteolytically inactive yeast Lon.
Proteomic analysis of mitochondrial protein turnover: identification of novel substrate proteins of the matrix protease pim1.
Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration.
The ATPase and protease domains of yeast mitochondrial Lon: roles in proteolysis and respiration-dependent growth.
The HAP3 regulatory locus of Saccharomyces cerevisiae encodes divergent overlapping transcripts.
The role of Lon-mediated proteolysis in the dynamics of mitochondrial nucleic acid-protein complexes.
Yeast mitochondrial ATP-dependent protease: purification and comparison with the homologous rat enzyme and the bacterial ATP-dependent protease La.
Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene.
A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae.
Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase.
Cryo-EM structure of the yeast respiratory supercomplex.
Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II.
Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2.
Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method.
Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme.
SOM1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae.
Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1.
Supercomplexes in the respiratory chains of yeast and mammalian mitochondria.
The cytochrome bc1 and cytochrome c oxidase complexes associate to form a single supracomplex in yeast mitochondria.
Use of the UGA terminator as a tryptophan codon in yeast mitochondria.
Chromatin remodeling by RSC involves ATP-dependent DNA translocation.
Functional differences between RSC1 and RSC2, components of a for growth essential chromatin-remodeling complex of Saccharomyces cerevisiae, during the sporulation process.
Histone octamer transfer by a chromatin-remodeling complex.
Multiple bromodomain genes are involved in restricting the spread of heterochromatic silencing at the Saccharomyces cerevisiae HMR-tRNA boundary.
RSC, an essential, abundant chromatin-remodeling complex.
RSC2, encoding a component of the RSC nucleosome remodeling complex, is essential for 2 micrometer plasmid maintenance in Saccharomyces cerevisiae.
The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation.
Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC.
Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains.
Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway.
Cox18p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner membrane.
Peripheral mitochondrial inner membrane protein, Mss2p, required for export of the mitochondrially coded Cox2p C tail in Saccharomyces cerevisiae.
The nuclear-encoded MSS2 gene is involved in the expression of the mitochondrial cytochrome-c oxidase subunit 2 (Cox2).
The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames.
ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes.
Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body.
Molecular analysis of the SNF8 gene of Saccharomyces cerevisiae.
Structure of the ESCRT-II endosomal trafficking complex.
A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases.
Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases.
Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast.
Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2.
The SIT4 protein phosphatase functions in late G1 for progression into S phase.
The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes.
Identification of a positive regulator of the cell cycle ubiquitin-conjugating enzyme Cdc34 (Ubc3).
Oscillatory nucleocytoplasmic shuttling of the general stress response transcriptional activators Msn2 and Msn4 in Saccharomyces cerevisiae.
The 9aaTAD transactivation domains: From Gal4 to p53.
The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).
Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae.
Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression.
Cloning and expression of two chitin deacetylase genes of Saccharomyces cerevisiae.
Expression, purification, and characterization of a cobalt-activated chitin deacetylase (Cda2p) from Saccharomyces cerevisiae.
Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli.
Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes.
Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3).
Purification of a ubiquitin protein peptidase from yeast with efficient in vitro assays.
Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p.
A panoramic view of yeast noncoding RNA processing.
Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII reveals the presence of eight open reading frames, including BRF1 (TFIIIB70) and GCN5 genes.
The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae.
Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily.
The SPS100 gene of Saccharomyces cerevisiae is activated late in the sporulation process and contributes to spore wall maturation.
Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme.
Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 from Saccharomyces cerevisiae.
Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae.
The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae.
A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4.
The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria.
A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult.
A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3.
Characterization of interaction sites in the Saccharomyces cerevisiae ribosomal stalk components.
Crystal structure of ADP-ribosylated ribosomal translocase from Saccharomyces cerevisiae.
Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
Elongation factor 2 as a novel target for selective inhibition of fungal protein synthesis.
Elongation factor methyltransferase 3 - A novel eukaryotic lysine methyltransferase.
Expression of non-ADP-ribosylatable, diphtheria toxin-resistant elongation factor 2 in Saccharomyces cerevisiae.
Isolation and properties of the trypsin-derived ADP-ribosyl peptide from diphtheria toxin-modified yeast elongation factor 2.
Methylation of translation-associated proteins in Saccharomyces cerevisiae: Identification of methylated lysines and their methyltransferases.
Phylogeny and evolution of medical species of Candida and related taxa: a multigenic analysis.
Protein identifications for a Saccharomyces cerevisiae protein database.
Saccharomyces cerevisiae elongation factor 2 is phosphorylated by an endogenous kinase.
Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling.
Saccharomyces cerevisiae elongation factor 2. Mutagenesis of the histidine precursor of diphthamide yields a functional protein that is resistant to diphtheria toxin.
Stoichiometry of Saccharomyces cerevisiae lysine methylation: insights into non-histone protein lysine methyltransferase activity.
Two crystal structures demonstrate large conformational changes in the eukaryotic ribosomal translocase.
Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes.
Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits.
TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients.
Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis.
Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis.
Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes.
Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue.
Effect of squalene synthase gene disruption on synthesis of polyprenols in Saccharomyces cerevisiae.
Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase.
Molecular cloning and characterization of the yeast gene for squalene synthetase.
Transcriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae.
Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme.
CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication.
Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication.
Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae.
A novel protein complex promoting formation of functional alpha- and gamma-tubulin.
Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonin-GimC system.
ATP22, a nuclear gene required for expression of the F0 sector of mitochondrial ATPase in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae ATP22 gene codes for the mitochondrial ATPase subunit 6-specific translation factor.
A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides.
Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae.
The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.
The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145.
Yeast TAFIIS in a multisubunit complex required for activated transcription.
Acta Crystallogr. F Struct. Biol. Commun.
Crystal structure of cytoplasmic acetoacetyl-CoA thiolase from Saccharomyces cerevisiae.
ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase.
Gene expression analysis of cold and freeze stress in Baker's yeast.
Transcriptional regulation of a sterol-biosynthetic enzyme by sterol levels in Saccharomyces cerevisiae.
Two forms of acetoacetyl coenzyme A thiolase in yeast. I. Separation and properties.
Two forms of acetoacetyl coenzyme A thiolase in yeast. II. Intracellular location and relationship to growth.
A newly characterized vacuolar serine carboxypeptidase, Atg42/Ybr139w, is required for normal vacuole function and the terminal steps of autophagy in the yeast Saccharomyces cerevisiae.
An analysis of the sequence of part of the right arm of chromosome II of S. cerevisiae reveals new genes encoding an amino-acid permease and a carboxypeptidase.
Dissection of glutathione conjugate turnover in yeast.
Identification of direct and indirect targets of the Gln3 and Gat1 activators by transcriptional profiling in response to nitrogen availability in the short and long term.
Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae.
The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes.
A family of eukaryotic lysophospholipid acyltransferases with broad specificity.
Biosynthesis of phosphatidic acid in lipid particles and endoplasmic reticulum of Saccharomyces cerevisiae.
Characterization of substrate preference for Slc1p and Cst26p in Saccharomyces cerevisiae using lipidomic approaches and an LPAAT activity assay.
LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.
Possible role of different yeast and plant lysophospholipid:acyl-CoA acyltransferases (LPLATs) in acyl remodelling of phospholipids.
SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast.
Genomic exploration of the hemiascomycetous yeasts: 4. The genome of Saccharomyces cerevisiae revisited.
Deletion of EFL1 results in heterogeneity of the 60 S GTPase-associated rRNA conformation.
Ria1p (Ynl163c), a protein similar to elongation factors 2, is involved in the biogenesis of the 60S subunit of the ribosome in Saccharomyces cerevisiae.
The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis.
Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores.
Budding yeast Cdc20: a target of the spindle checkpoint.
Feedback control of mitosis in budding yeast.
The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase.
The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins.
Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae.
A genomewide screen for petite-negative yeast strains yields a new subunit of the i-AAA protease complex.
Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S. cerevisiae.
Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex.
Inactivation of YME1, a member of the ftsH-SEC18-PAS1-CDC48 family of putative ATPase-encoding genes, causes increased escape of DNA from mitochondria in Saccharomyces cerevisiae.
Mgr3p and Mgr1p are adaptors for the mitochondrial i-AAA protease complex.
Multiple genes, including a member of the AAA family, are essential for degradation of unassembled subunit 2 of cytochrome c oxidase in yeast mitochondria.
Substrate specific consequences of central pore mutations in the i-AAA protease Yme1 on substrate engagement.
The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry.
A family of yeast proteins mediating bidirectional vacuolar amino acid transport.
Identification of a 60S preribosomal particle that is closely linked to nuclear export.
Evolution of gene families: the multidrug resistance transporter genes in five related yeast species.
Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae.
A novel subunit of yeast RNA polymerase III interacts with the TFIIB-related domain of TFIIIB70.
An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.
A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
Dual role of mitofilin in mitochondrial membrane organization and protein biogenesis.
MINOS1 is a conserved component of mitofilin complexes and required for mitochondrial function and cristae organization.
The mitochondrial contact site complex, a determinant of mitochondrial architecture.
Uniform nomenclature for the mitochondrial contact site and cristae organizing system.
An integrated mass spectrometry-based proteomic approach: quantitative analysis of tandem affinity-purified in vivo cross-linked protein complexes (QTAX) to decipher the 26 S proteasome-interacting network.
Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.
Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins.
The H2O2 stimulon in Saccharomyces cerevisiae.
Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny.
Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM.
Promotor mutants of the yeast his3 gene.
Saturation mutagenesis of the yeast his3 regulatory site: requirements for transcriptional induction and for binding by GCN4 activator protein.
Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast.
Essential role for the myotubularin-related phosphatase Ymr1p and the synaptojanin-like phosphatases Sjl2p and Sjl3p in regulation of phosphatidylinositol 3-phosphate in yeast.
Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiae.
Interaction of Pik1p and Sjl proteins in membrane trafficking.
Mammalian inositol polyphosphate 5-phosphatase II can compensate for the absence of all three yeast Sac1-like-domain-containing 5-phosphatases.
Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant.
PtdIns(4,5)P2 turnover is required for multiple stages during clathrin- and actin-dependent endocytic internalization.
SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases.
SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases.
Sjl2p is specifically involved in early steps of endocytosis intimately linked to actin dynamics via the Ark1p/Prk1p kinases.
Synaptojanin family members are implicated in endocytic membrane traffic in yeast.
The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis.
The phosphoinositide phosphatase Sjl2 is recruited to cortical actin patches in the control of vesicle formation and fission during endocytosis.
The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames.
The yeast inositol polyphosphate 5-phosphatases inp52p and inp53p translocate to actin patches following hyperosmotic stress: mechanism for regulating phosphatidylinositol 4,5-bisphosphate at plasma membrane invaginations.
Genes of succinyl-CoA ligase from Saccharomyces cerevisiae.
A gene from Saccharomyces cerevisiae which codes for a protein with significant homology to the bacterial 3-phosphoserine aminotransferase.
Molecular analysis of the yeast SER1 gene encoding 3-phosphoserine aminotransferase: regulation by general control and serine repression.
A conserved deubiquitinating enzyme controls cell growth by regulating RNA polymerase I stability.
A conserved deubiquitinating enzyme uses intrinsically disordered regions to scaffold multiple protein interaction sites.
Der1, a novel protein specifically required for endoplasmic reticulum degradation in yeast.
Der1p, a protein required for degradation of malfolded soluble proteins of the endoplasmic reticulum: topology and Der1-like proteins.
Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins.
N-terminal acetylation of the yeast Derlin Der1 is essential for Hrd1 ubiquitin-ligase activity toward luminal ER substrates.
Structural basis of ER-associated protein degradation mediated by the Hrd1 ubiquitin ligase complex.
Usa1 functions as a scaffold of the HRD-ubiquitin ligase.
Functional interactions between yeast mitochondrial ribosomes and mRNA 5' untranslated leaders.
Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR.
Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI.
Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions.
The yeast mitochondrial carrier Leu5p and its human homologue Graves' disease protein are required for accumulation of coenzyme A in the matrix.
Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification.
Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I.
Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class of alpha-tubulin mutations that arrest with excess microtubules.
Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification.
Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2.
Yeast alpha-tubulin suppressor ATS1/KTI13 relates to the Elongator complex and interacts with Elongator partner protein Kti11.
A survey of essential gene function in the yeast cell division cycle.
NQR1 controls lifespan by regulating the promotion of respiratory metabolism in yeast.
Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae.
The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping.
Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease.
Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B' protein.
The yeast U2A'/U2B complex is required for pre-spliceosome formation.
SDHAF1, encoding a LYR complex-II specific assembly factor, is mutated in SDH-defective infantile leukoencephalopathy.
The LYR factors SDHAF1 and SDHAF3 mediate maturation of the iron-sulfur subunit of succinate dehydrogenase.
Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone.
Genomic analysis of PIS1 gene expression.
A mutant with a defect in telomere elongation leads to senescence in yeast.
New function of CDC13 in positive telomere length regulation.
Playing with blocks: some pitfalls of forcing multiple alignments.
RNA-dependent polymerase motifs in EST1: tentative identification of a protein component of an essential yeast telomerase.
The nuclear envelope and spindle pole body-associated Mps3 protein bind telomere regulators and function in telomere clustering.
Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae.
A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis.
Genetic analysis of growth inhibition by GAL4-L kappa B-alpha in Saccharomyces cerevisiae.
Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product.
A novel cold-sensitive allele of the rate-limiting enzyme of fatty acid synthesis, acetyl coenzyme A carboxylase, affects the morphology of the yeast vacuole through acylation of Vac8p.
A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex.
Acetyl-CoA carboxylase from yeast is an essential enzyme and is regulated by factors that control phospholipid metabolism.
Analysis of FAS3/ACC regulatory region of Saccharomyces cerevisiae: identification of a functional UASINO and sequences responsible for fatty acid mediated repression.
Cloning of the yeast FAS3 gene and primary structure of yeast acetyl-CoA carboxylase.
Coordinated regulation and inositol-mediated and fatty acid-mediated repression of fatty acid synthase genes in Saccharomyces cerevisiae.
Crystal structure of the carboxyltransferase domain of acetyl-coenzyme A carboxylase.
Fatty acid-requiring mutant of Saccharomyces cerevisiae defective in acetyl-CoA carboxylase.
Identification of the yeast ACC1 gene product (acetyl-CoA carboxylase) as the target of the polyketide fungicide soraphen A.
Molecular basis for the inhibition of the carboxyltransferase domain of acetyl-coenzyme-A carboxylase by haloxyfop and diclofop.
Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p.
Yeast acetyl-CoA carboxylase is associated with the cytoplasmic surface of the endoplasmic reticulum.
Yeast mutants defective in acetyl-coenzyme A carboxylase and biotin: apocarboxylase ligase.
A novel gene conserved from yeast to humans is involved in sterol biosynthesis.
The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p).
Dependence of yeast pre-mRNA 3'-end processing on CFT1: a sequence homolog of the mammalian AAUAAA binding factor.
Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends.
Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.
Differential effects of chromatin and Gcn4 on the 50-fold range of expression among individual yeast Ty1 retrotransposons.
A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p.
Genome-wide mRNA surveillance is coupled to mRNA export.
Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome.
Rrp47p is an exosome-associated protein required for the 3' processing of stable RNAs.
Rrp6p, the yeast homologue of the human PM-Scl 100-kDa autoantigen, is essential for efficient 5.8 S rRNA 3' end formation.
The sequence of a 30 kb fragment on the left arm of chromosome XV from Saccharomyces cerevisiae reveals 15 open reading frames, five of which correspond to previously identified genes.
Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability.
Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
Candida albicans Ssa1/2p is the cell envelope binding protein for human salivary histatin 5.
Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae.
The SSA1 and SSA2 genes of the yeast Saccharomyces cerevisiae.
The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein.
Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids.
HSP12 is essential for biofilm formation by a Sardinian wine strain of Saccharomyces cerevisiae.
HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function.
The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.
The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames.
YGR198w (YPP1) targets A30P alpha-synuclein to the vacuole for degradation.
Analysis of the genes activated by the FLO8 gene in Saccharomyces cerevisiae.
Karyopherin-mediated import of integral inner nuclear membrane proteins.
SGT2 and MDY2 interact with molecular chaperone YDJ1 in Saccharomyces cerevisiae.
Small glutamine-rich protein/viral protein U-binding protein is a novel cochaperone that affects heat shock protein 70 activity.
MNN6, a member of the KRE2/MNT1 family, is the gene for mannosylphosphate transfer in Saccharomyces cerevisiae.
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.
The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing.
Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding.
A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes.
Subcellular localization of Axl1, the cell type-specific regulator of polarity.
Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.
Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates.
Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK.
Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast.
Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae.
Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms.
Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes.
Escherichia coli and Saccharomyces cerevisiae acetylornithine aminotransferase: evolutionary relationship with ornithine aminotransferase.
Sequence analysis of a 9873 bp fragment of the left arm of yeast chromosome XV that contains the ARG8 and CDC33 genes, a putative riboflavin synthase beta chain gene, and four new open reading frames.
Sequencing of a 13.2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V.
The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane.
Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth.
Autocrine activation of the pheromone response pathway in matalpha2-cells is attenuated by SST2- and ASG7-dependent mechanisms.
Identification of target sites of the alpha2-Mcm1 repressor complex in the yeast genome.
Localization and signaling of G(beta) subunit Ste4p are controlled by a-factor receptor and the a-specific protein Asg7p.
Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV.
Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.
Yeast ribosomal proteins: XI. Molecular analysis of two genes encoding YL41, an extremely small and basic ribosomal protein, from Saccharomyces cerevisiae.
DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII.
Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae.
Purification and characterization of yeast RNA polymerase II general initiation factor g.
Multiprotein transcription factor UAF interacts with the upstream element of the yeast RNA polymerase I promoter and forms a stable preinitiation complex.
Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription.
A small conserved domain in the yeast Spa2p is necessary and sufficient for its polarized localization.
Identification and characterization of upstream open reading frames (uORF) in the 5' untranslated regions (UTR) of genes in Saccharomyces cerevisiae.
Role of a ubiquitin-like modification in polarized morphogenesis.
The Spa2-related protein, Sph1p, is important for polarized growth in yeast.
Isolation of a cDNA likely to encode a novel Ca2+-dependent/calmodulin-stimulated protein phosphatase.
The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B.
Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase.
A new essential gene located on Saccharomyces cerevisiae chromosome IX.
Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion.
Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae.
Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.
Copper supplementation restores cytochrome c oxidase assembly defect in a mitochondrial disease model of COA6 deficiency.
Genome-wide analysis of eukaryotic twin CX9C proteins.
Genome-wide analysis of membrane targeting by S.cerevisiae pleckstrin homology domains.
A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration.
ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued.
Arp10p is a pointed-end-associated component of yeast dynactin.
Dynactin is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae.
Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae.
The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B.
A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies.
Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast.
Aberrant 3' oligoadenylation of spliceosomal U6 small nuclear RNA in poikiloderma with neutropenia.
C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3' end modification.
Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities.
ATP 10, a yeast nuclear gene required for the assembly of the mitochondrial F1-F0 complex.
Global analysis of protein palmitoylation in yeast.
Immunoisolation of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments.
Sorting of proteins into multivesicular bodies: ubiquitin-dependent and -independent targeting.
Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames.
Molecular architecture of the TRAPPII complex and implications for vesicle tethering.
TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport.
TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion.
The role of Trs65 in the Ypt/Rab guanine nucleotide exchange factor function of the TRAPP II complex.
Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats.
Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae.
Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis.
Tom71, a novel homologue of the mitochondrial preprotein receptor Tom70.
The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase.
A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP.
A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA.
Analysis of Snu13p mutations reveals differential interactions with the U4 snRNA and U3 snoRNA.
Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro.
Structural comparison of yeast snoRNP and spliceosomal protein Snu13p with its homologs.
Assembly of the mitochondrial membrane system. MRP1 and MRP2, two yeast nuclear genes coding for mitochondrial ribosomal proteins.
Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein.
A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast.
Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae.
New insights into gamma-aminobutyric acid catabolism: evidence for gamma-hydroxybutyric acid and polyhydroxybutyrate synthesis in Saccharomyces cerevisiae.
Nucleotide sequence of the yeast UGA1 gene encoding GABA transaminase.
Subcellular localization of the yeast proteome.
Genetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes.
The fungal alpha-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate.
Nop53p is required for late 60S ribosome subunit maturation and nuclear export in yeast.
Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae.
A genome-wide immunodetection screen in S. cerevisiae uncovers novel genes involved in lysosomal vacuole function and morphology.
Direct physical and functional interaction of the NuA4 complex components Yaf9p and Swc4p.
Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4.
The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres.
Structure and calcium-binding properties of Frq1, a novel calcium sensor in the yeast Saccharomyces cerevisiae.
Yeast homologue of neuronal frequenin is a regulator of phosphatidylinositol-4-OH kinase.
A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae.
A highly redundant gene network controls assembly of the outer spore wall in S. cerevisiae.
A screen for spore wall permeability mutants identifies a secreted protease required for proper spore wall assembly.
Rfm1, a novel tethering factor required to recruit the hst1 histone deacetylase for repression of middle sporulation genes.
Mdm38 is a 14-3-3-like receptor and associates with the protein synthesis machinery at the inner mitochondrial membrane.
Ribosome-binding proteins Mdm38 and Mba1 display overlapping functions for regulation of mitochondrial translation.
The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome.
The PET18 locus of Saccharomyces cerevisiae: a complex locus containing multiple genes.
Homomeric ring assemblies of eukaryotic Sm proteins have affinity for both RNA and DNA. Crystal structure of an oligomeric complex of yeast SmF.
Interactions within the yeast Sm core complex: from proteins to amino acids.
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs.
Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain.
Altered sterol metabolism in budding yeast affects mitochondrial iron-sulfur (Fe-S) cluster synthesis.
Regulation of mitochondrial iron accumulation by Yfh1p, a putative homolog of frataxin.
Role of essential genes in mitochondrial morphogenesis in Saccharomyces cerevisiae.
The essential gene YMR134W from Saccharomyces cerevisiae is important for appropriate mitochondrial iron utilization and the ergosterol biosynthetic pathway.
Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome.
Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning.
Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.
Sir-mediated repression can occur independently of chromosomal and subnuclear contexts.
Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component.
Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP.
Rpp2, an essential protein subunit of nuclear RNase P, is required for processing of precursor tRNAs and 35S precursor rRNA in Saccharomyces cerevisiae.
Functional analysis of the sporulation-specific SPR6 gene of Saccharomyces cerevisiae.
Activation and localization of inositol phosphosphingolipid phospholipase C, Isc1p, to the mitochondria during growth of Saccharomyces cerevisiae.
Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae.
Isc1 regulates sphingolipid metabolism in yeast mitochondria.
A two-component system that regulates an osmosensing MAP kinase cascade in yeast.
Crystal structure of the Rad3/XPD regulatory domain of Ssl1/p44.
Different forms of TFIIH for transcription and DNA repair: holo-TFIIH and a nucleotide excision repairosome.
Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair.
RNA polymerase transcription factor IIH holoenzyme from yeast.
Reconstitution of TFIIH and requirement of its DNA helicase subunits, Rad3 and Rad25, in the incision step of nucleotide excision repair.
Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH.
SSL1, a suppressor of a HIS4 5'-UTR stem-loop mutation, is essential for translation initiation and affects UV resistance in yeast.
Characterization of mammalian eIF2A and identification of the yeast homolog.
Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A.
Quantitative trait loci mapped to single-nucleotide resolution in yeast.
Amino acid homeostatic control by TORC1 in Saccharomyces cerevisiae under high hydrostatic pressure.
Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8.
An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase.
Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes.
Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for chromosome segregation in Saccharomyces cerevisiae.
Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex.
Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi.
Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface.
Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast.
Disulfide bond structure and domain organization of yeast beta(1,3)-glucanosyltransferases involved in cell wall biogenesis.
Molecular mechanisms of yeast cell wall glucan remodeling.
Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase.
Saccharomyces cerevisiae Yak1p protein kinase autophosphorylates on tyrosine residues and phosphorylates myelin basic protein on a C-terminal serine residue.
Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI.
Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal.
Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus.
Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit.
Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae.
Identification of a putative amidase gene in yeast Saccharomyces cerevisiae.
Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1.
Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants.
Function of the ste signal transduction pathway for mating pheromones sustains MAT alpha 1 transcription in Saccharomyces cerevisiae.
Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex.
NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization.
Nucleotide sequence of the yeast cell division cycle start genes CDC28, CDC36, CDC37, and CDC39, and a structural analysis of the predicted products.
The nucleotide sequence of the DNA ligase gene (CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage.
A role for Ctr9p and Paf1p in the regulation of G1 cyclin expression in yeast.
Cdc73p and Paf1p are found in a novel RNA polymerase II-containing complex distinct from the Srbp-containing holoenzyme.
RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach.
Philos. Trans. R. Soc. Lond., B, Biol. Sci.
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.
Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro.
Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins.
SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis.
Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.
Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system.
Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading.
A role for transcriptional repressors in targeting the yeast Swi/Snf complex.
Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae.
Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae.
Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing.
Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure.
Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae.
Functional dissection of yeast Hir1p, a WD repeat-containing transcriptional corepressor.
Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae.
Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex.
Hir proteins are required for position-dependent gene silencing in Saccharomyces cerevisiae in the absence of chromatin assembly factor I.
Hir1p and Hir2p function as transcriptional corepressors to regulate histone gene transcription in the Saccharomyces cerevisiae cell cycle.
Histones are incorporated in trans during reassembly of the yeast PHO5 promoter.
Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae.
Replication-independent histone deposition by the HIR complex and Asf1.
Sequence of a 12.7 kb segment of yeast chromosome II identifies a PDR-like gene and several new open reading frames.
The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF.
The budding yeast silencing protein Sir1 is a functional component of centromeric chromatin.
Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression.
Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription.
Yeast Ty1 retrotransposition is stimulated by a synergistic interaction between mutations in chromatin assembly factor I and histone regulatory proteins.
Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing.
Sequence of the Saccharomyces GAL region and its transcription in vivo.
Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae.
Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1.
A systematic survey identifies prions and illuminates sequence features of prionogenic proteins.
Combinatorial repression of the hypoxic genes of Saccharomyces cerevisiae by DNA binding proteins Rox1 and Mot3.
Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae.
Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae.
Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae.
Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast.
Synergistic repression of anaerobic genes by Mot3 and Rox1 in Saccharomyces cerevisiae.
Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis.
Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH. Homology to human cyclin-dependent kinase activating kinase and IIH subunits.
Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation.
Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast.
Genetic dissection of the budding yeast Arp2/3 complex: a comparison of the in vivo and structural roles of individual subunits.
Dsl1p, an essential component of the Golgi-endoplasmic reticulum retrieval system in yeast, uses the same sequence motif to interact with different subunits of the COPI vesicle coat.
Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast.
Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit.
The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast.
New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis.
A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue.
The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits.
Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker.
Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively.
Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex.
Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae.
A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames.
Chemotyping of yeast mutants using robotics.
WD40 repeat propellers define a ubiquitin-binding domain that regulates turnover of F box proteins.
Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme.
Identification and characterization of a second NMN adenylyltransferase gene in Saccharomyces cerevisiae.
Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels.
Nicotinamide mononucleotide adenylyltransferase. Molecular and enzymatic properties of the homogeneous enzyme from baker's yeast.
The deamido-diphosphopyridine nucleotide and diphosphopyridine nucleotide pyrophosphorylases of Escherichia coli and yeast.
The participation of inorganic pyrophosphate in the reversible enzymatic synthesis of diphosphopyridine nucleotide.
YCL047C/POF1 is a novel nicotinamide mononucleotide adenylyltransferase (NMNAT) in Saccharomyces cerevisiae.
In vitro binding to the leucine tRNA gene identifies a novel yeast homeobox gene.
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
The condensin complex governs chromosome condensation and mitotic transmission of rDNA.
A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturation.
A novel eukaryotic factor for cytosolic Fe-S cluster assembly.
Functional link between ribosome formation and biogenesis of iron-sulfur proteins.
Mitochondria export iron-sulfur and sulfur intermediates to the cytoplasm for iron-sulfur cluster assembly and tRNA thiolation in yeast.
The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.
Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase.
Kinetic and thermodynamic framework for assembly of the six-component bI3 group I intron ribonucleoprotein catalyst.
Protein encoded by the third intron of cytochrome b gene in Saccharomyces cerevisiae is an mRNA maturase. Analysis of mitochondrial mutants, RNA transcripts, proteins and evolutionary relationships.
Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA.
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway.
Phenylalanine- and tyrosine-auxotrophic mutants of Saccharomyces cerevisiae impaired in transamination.
Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae.
Tryptophan degradation in Saccharomyces cerevisiae: characterization of two aromatic aminotransferases.
Identification of yeast proteins necessary for cell-surface function of a potassium channel.
A physical and functional interaction between yeast Pol4 and Dnl4-Lif1 links DNA synthesis and ligation in nonhomologous end joining.
NHEJ regulation by mating type is exercised through a novel protein, Lif2p, essential to the ligase IV pathway.
Saccharomyces cerevisiae LIF1: a function involved in DNA double-strand break repair related to mammalian XRCC4.
Structure of an Xrcc4-DNA ligase IV yeast ortholog complex reveals a novel BRCT interaction mode.
Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae.
XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination.
N-terminal acetylome analysis reveals the specificity of Naa50 (Nat5) and suggests a kinetic competition between N-terminal acetyltransferases and methionine aminopeptidases.
The yeast N(alpha)-acetyltransferase NatA is quantitatively anchored to the ribosome and interacts with nascent polypeptides.
Identification of amino acid changes affecting yeast uroporphyrinogen decarboxylase activity by sequence analysis of hem12 mutant alleles.
Molecular analysis of HEM6 (HEM12) in Saccharomyces cerevisiae, the gene for uroporphyrinogen decarboxylase.
Uroporphyrinogen decarboxylase in Saccharomyces cerevisiae. HEM12 gene sequence and evidence for two conserved glycines essential for enzymatic activity.
A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.
A comprehensive two-hybrid analysis to explore the yeast protein interactome.
Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded.
Peering through the pore: nuclear pore complex structure, assembly, and function.
Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p.
Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae.
HDA1 and HDA3 are components of a yeast histone deacetylase (HDA) complex.
HDA2 and HDA3 are related proteins that interact with and are essential for the activity of the yeast histone deacetylase HDA1.
Hsl7 is a substrate-specific type II protein arginine methyltransferase in yeast.
Hsl7 localizes to a septin ring and serves as an adapter in a regulatory pathway that relieves tyrosine phosphorylation of Cdc28 protein kinase in Saccharomyces cerevisiae.
Hsl7p, a negative regulator of Ste20p protein kinase in the Saccharomyces cerevisiae filamentous growth-signaling pathway.
Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase.
The morphogenesis checkpoint in Saccharomyces cerevisiae: cell cycle control of Swe1p degradation by Hsl1p and Hsl7p.
Yeast Hsl7 (histone synthetic lethal 7) catalyses the in vitro formation of omega-N(G)-monomethylarginine in calf thymus histone H2A.
A targeted bypass screen identifies Ynl187p, Prp42p, Snu71p, and Cbp80p for stable U1 snRNP/Pre-mRNA interaction.
The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.
Cloning of the multicopy suppressor gene SUR7: evidence for a functional relationship between the yeast actin-binding protein Rvs167 and a putative membranous protein.
Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells.
Eisosomes mark static sites of endocytosis.
Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae.
The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation.
A yeast mitochondrial membrane methyltransferase-like protein can compensate for oxa1 mutations.
Automated identification of putative methyltransferases from genomic open reading frames.
Molecular cloning, characterization and analysis of the regulation of the ARO2 gene, encoding chorismate synthase, of Saccharomyces cerevisiae.
Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase.
STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.
Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway.
Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex.
The solution structure of the S.cerevisiae Ste11 MAPKKK SAM domain and its partnership with Ste50.
A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex.
Accelerating the rate of disassembly of karyopherin-cargo complexes.
Chromatin boundaries in budding yeast: the nuclear pore connection.
Deciphering networks of protein interactions at the nuclear pore complex.
GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta.
Minimal nuclear pore complexes define FG repeat domains essential for transport.
Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm.
NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.
Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex.
Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha.
Proteomic analysis of nucleoporin interacting proteins.
Structural basis for Nup2p function in cargo release and karyopherin recycling in nuclear import.
The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex.
A cycling protein complex required for selective autophagy.
Atg27 is required for autophagy-dependent cycling of Atg9.
Atg9 vesicles are an important membrane source during early steps of autophagosome formation.
Atg9 vesicles recruit vesicle-tethering proteins Trs85 and Ypt1 to the autophagosome formation site.
Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy.
DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase.
Identification of regulatory residues of the yeast adenylyl cyclase.
Yeast adenylate cyclase catalytic domain is carboxy terminal.
Coordinate regulation of glycogen metabolism in the yeast Saccharomyces cerevisiae. Induction of glycogen branching enzyme.
The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase.
Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae.
Discovery and Functional Characterization of a Yeast Sugar Alcohol Phosphatase.
RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif.
The isolation, characterization and nucleotide sequence of the phosphoglucoisomerase gene of Saccharomyces cerevisiae.
The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae.
Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation.
A novel and conserved salt-induced protein is an important determinant of salt tolerance in yeast.
Nucleotide sequence of the gene encoding cytochrome c oxidase subunit VII from Saccharomyces cerevisiae.
The nuclear-coded subunits of yeast cytochrome c oxidase. The amino acid sequences of subunits VII and VIIa, structural similarities between the three smallest polypeptides of the holoenzyme, and implications for biogenesis.
Yeast cytochrome c oxidase subunit VII is essential for assembly of an active enzyme. Cloning, sequencing, and characterization of the nuclear-encoded gene.
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.
Studies on yeast sulfite reductase. IV. Structure and steady-state kinetics.
A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants.
Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway.
The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae.
A yeast t-SNARE involved in endocytosis.
The Sec1p homologue Vps45p binds to the syntaxin Tlg2p.
Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures.
A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin.
Identification of mitogen-activated protein kinase signaling pathways that confer resistance to endoplasmic reticulum stress in Saccharomyces cerevisiae.
Elevated levels of a U4/U6.U5 snRNP-associated protein, Spp381p, rescue a mutant defective in spliceosome maturation.
The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis.
Yeh1 constitutes the major steryl ester hydrolase under heme-deficient conditions in Saccharomyces cerevisiae.
Identification of phosphorylation sites on the yeast ribonucleotide reductase inhibitor Sml1.
Sml1p is a dimer in solution: characterization of denaturation and renaturation of recombinant Sml1p.
The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1.
The ribonucleotide reductase inhibitor, Sml1, is sequentially phosphorylated, ubiquitylated and degraded in response to DNA damage.
Yeast Sml1, a protein inhibitor of ribonucleotide reductase.
Architecture of initiation-competent 12-subunit RNA polymerase II.
Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage.
Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS.
Dissociable Rpb4-Rpb7 subassembly of RNA polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation.
Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.
Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model.
RNA polymerase II/TFIIF structure and conserved organization of the initiation complex.
RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability.
Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.
Structure and mechanism of RNA polymerase II CTD phosphatases.
Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs.
Structures of complete RNA polymerase II and its subcomplex, Rpb4/7.
The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport.
Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy.
The evolutionary conserved BER1 gene is involved in microtubule stability in yeast.
Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue.
The monocarboxylate transporter homolog Mch5p catalyzes riboflavin (vitamin B2) uptake in Saccharomyces cerevisiae.
Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae.
Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae.
A conserved membrane-binding domain targets proteins to organelle contact sites.
A phospholipid transfer function of ER-mitochondria encounter structure revealed in vitro.
ER-mitochondrial junctions can be bypassed by dominant mutations in the endosomal protein Vps13.
MMM1 encodes a mitochondrial outer membrane protein essential for establishing and maintaining the structure of yeast mitochondria.
Mmm1p spans both the outer and inner mitochondrial membranes and contains distinct domains for targeting and foci formation.
Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability.
Mmm2p, a mitochondrial outer membrane protein required for yeast mitochondrial shape and maintenance of mtDNA nucleoids.
Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a 43.7 kb fragment of chromosome XII including an open reading frame homologous to the human cystic fibrosis transmembrane conductance regulator protein CFTR.
Yeast Vps13 promotes mitochondrial function and is localized at membrane contact sites.
Constitutive flocculation in Saccharomyces cerevisiae through overexpression of the GTS1 gene, coding for a 'Glo'-type Zn-finger-containing protein.
The GTS1 gene, which contains a Gly-Thr repeat, affects the timing of budding and cell size of the yeast Saccharomyces cerevisiae.
Shs1p: a novel member of septin that interacts with spa2p, involved in polarized growth in Saccharomyces cerevisiae.
The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating.
Control of cellular physiology by TM9 proteins in yeast and Dictyostelium.
Transmembrane nine proteins in yeast and Arabidopsis affect cellular metal contents without changing vacuolar morphology.
Identification of the yeast ARG-11 gene as a mitochondrial ornithine carrier involved in arginine biosynthesis.
The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis.
Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae.
Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae.
Protein interaction networks by proteome peptide scanning.
SCP1 encodes an actin-bundling protein in yeast.
The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton.
HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription.
Yeast HOS3 forms a novel trichostatin A-insensitive homodimer with intrinsic histone deacetylase activity.
Logic of the yeast metabolic cycle: temporal compartmentalization of cellular processes.
Presence and potential signaling function of N-acylethanolamines and their phospholipid precursors in the yeast Saccharomyces cerevisiae.
ASC1/RAS2 suppresses the growth-defect on glycerol caused by the atp1-2 mutation in the yeast Saccharomyces cerevisiae.
Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
Genetic analysis of yeast RAS1 and RAS2 genes.
Modulation of Ras and a-factor function by carboxyl-terminal proteolysis.
Nucleotide sequence of two rasH related-genes isolated from the yeast Saccharomyces cerevisiae.
RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins.
RAS2 protein of Saccharomyces cerevisiae is methyl-esterified at its carboxyl terminus.
RAS2 protein of Saccharomyces cerevisiae undergoes removal of methionine at N-terminus and removal of three amino acids at C terminus.
S-farnesylation and methyl esterification of C-terminal domain of yeast RAS2 protein prior to fatty acid acylation.
Identification of core components and transient interactors of the peroxisomal importomer by dual-track stable isotope labeling with amino acids in cell culture analysis.
Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses.
Structure and functional analysis of the multistress response gene DDR2 from Saccharomyces cerevisiae.
Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces cerevisiae.
Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae.
A heritable switch in carbon source utilization driven by an unusual yeast prion.
Dissection of functional domains of the yeast proton-pumping ATPase by directed mutagenesis.
The ATP binding site of the yeast plasma membrane proton-translocating ATPase.
Two novel effectors of trafficking and maturation of the yeast plasma membrane H+ -ATPase.
Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases.
Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
A Sm-like protein complex that participates in mRNA degradation.
Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover.
Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.
Crystal structure and biochemical analysis of the heptameric Lsm1-7 complex.
The two proteins Pat1p (Mrt1p) and Spb8p interact in vivo, are required for mRNA decay, and are functionally linked to Pab1p.
Yeast Sm-like proteins function in mRNA decapping and decay.
The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme.
The Ubp2 deubiquitinating enzyme modulates Rsp5-dependent K63-linked polyubiquitin conjugates in Saccharomyces cerevisiae.
Interaction of alpha-agglutinin and a-agglutinin, Saccharomyces cerevisiae sexual cell adhesion molecules.
Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin.
Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction.
Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6.
A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP.
A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution.
Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction.
Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues.
Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein-related proteins and phosphoinositides.
Osh proteins regulate COPII-mediated vesicular transport of ceramide from the endoplasmic reticulum in budding yeast.
Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sites.
Overlapping functions of the yeast oxysterol-binding protein homologues.
Structure of Osh3 reveals a conserved mode of phosphoinositide binding in oxysterol-binding proteins.
The GOLD domain, a novel protein module involved in Golgi function and secretion.
An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms.
Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I.
Translation of a yeast mitochondrial tRNA synthetase initiated at redundant non-AUG codons.
Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.
Identification and characterization of the CLK1 gene product, a novel CaM kinase-like protein kinase from the yeast Saccharomyces cerevisiae.
Rck2 kinase is a substrate for the osmotic stress-activated mitogen-activated protein kinase Hog1.
Two novel deduced serine/threonine protein kinases from Saccharomyces cerevisiae.
Primary structure and function of a second essential member of the heterooligomeric TCP1 chaperonin complex of yeast, TCP1 beta.
The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes.
The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p.
Composition of the spindle pole body of Saccharomyces cerevisiae and the proteins involved in its duplication.
Direct interaction between yeast spindle pole body components: Kar1p is required for Cdc31p localization to the spindle pole body.
Fine structure analysis of the yeast centrin, Cdc31p, identifies residues specific for cell morphology and spindle pole body duplication.
Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae.
Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p.
Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication.
The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body.
The Vps13p-Cdc31p complex is directly required for TGN late endosome transport and TGN homotypic fusion.
Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.
L-arginine recognition by yeast arginyl-tRNA synthetase.
tRNA aminoacylation by arginyl-tRNA synthetase: induced conformations during substrates binding.
Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae.
Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli.
Isolation and characterization of sulfite mutants of Saccharomyces cerevisiae.
Molecular characterization of a chromosomal rearrangement involved in the adaptive evolution of yeast strains.
Population structure and gene evolution in Saccharomyces cerevisiae.
SSU1 encodes a plasma membrane protein with a central role in a network of proteins conferring sulfite tolerance in Saccharomyces cerevisiae.
SSU1 mediates sulphite efflux in Saccharomyces cerevisiae.
Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA.
Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast.
The DEAD-box RNA helicase-like Utp25 is an SSU processome component.
A novel superfamily of bridge-like lipid transfer proteins.
Vps13-like proteins provide phosphatidylethanolamine for GPI anchor synthesis in the ER.
A highly conserved intraspecies homolog of the Saccharomyces cerevisiae elongation factor-3 encoded by the HEF3 gene.
Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress.
Identification and kinetic analysis of a functional homolog of elongation factor 3, YEF3 in Saccharomyces cerevisiae.
Cargo sequences are important for Som1p-dependent signal peptide cleavage in yeast mitochondria.
Analysis of a 36.2 kb DNA sequence including the right telomere of chromosome VI from Saccharomyces cerevisiae.
Dug1p Is a Cys-Gly peptidase of the gamma-glutamyl cycle of Saccharomyces cerevisiae and represents a novel family of Cys-Gly peptidases.
The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae.
A nuclear protein with sequence similarity to proteins implicated in human acute leukemias is important for cellular morphogenesis and actin cytoskeletal function in Saccharomyces cerevisiae.
An essential yeast gene encoding a TTAGGG repeat-binding protein.
Association of Taf14 with acetylated histone H3 directs gene transcription and the DNA damage response.
Molecular genetics of the RNA polymerase II general transcriptional machinery.
Recent advances in understanding chromatin remodeling by SWI/SNF complexes.
Structural analysis of the yeast SWI/SNF chromatin remodeling complex.
Structural insights into the pi-pi-pi stacking mechanism and DNA-binding activity of the YEATS domain.
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex.
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.
Characterization of a high mobility group 1/2 homolog in yeast.
Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae.
Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.
The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.
The conformation of a-factor is not influenced by the S-prenylation of Cys12.
Genome-wide expression analysis of NAP1 in Saccharomyces cerevisiae.
Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus.
Gene transcription analysis of Saccharomyces cerevisiae exposed to neocarzinostatin protein-chromophore complex reveals evidence of DNA damage, a potential mechanism of resistance, and consequences of prolonged exposure.
Identification of a novel system for boron transport: Atr1 is a main boron exporter in yeast.
Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH4+ transport.
Elucidation of the deficiency in two yeast coenzyme Q mutants. Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase.
Sequence around the centromere of Saccharomyces cerevisiae chromosome II: similarity of CEN2 to CEN4.
Genetic analysis reveals that FLO11 upregulation and cell polarization independently regulate invasive growth in Saccharomyces cerevisiae.
Crystal structure of a PPR1-DNA complex: DNA recognition by proteins containing a Zn2Cys6 binuclear cluster.
Yeast regulatory gene PPR1. I. Nucleotide sequence, restriction map and codon usage.
Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions.
ATP synthase of yeast mitochondria. Isolation of the F1 delta subunit, sequence and disruption of the structural gene.
Molecular architecture of the rotary motor in ATP synthase.
90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors.
A single acetylation of 18 S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae.
Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1.
The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control.
A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins.
Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase.
Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains.
Coupled synthesis and translocation restrains polyphosphate to acidocalcisome-like vacuoles and prevents its toxicity.
Inositol pyrophosphate specificity of the SPX-dependent polyphosphate polymerase VTC.
Role of the Vtc proteins in V-ATPase stability and membrane trafficking.
The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation.
The macro domain as fusion tag for carrier-driven crystallization.
The vacuolar transporter chaperone (VTC) complex is required for microautophagy.
Trypanosoma brucei vacuolar transporter chaperone 4 (TbVtc4) is an acidocalcisome polyphosphate kinase required for in vivo infection.
Vtc5, a novel subunit of the vacuolar transporter chaperone complex, regulates polyphosphate synthesis and phosphate homeostasis in yeast.
Cloning and genetic analysis of the gene encoding a new protein kinase in Saccharomyces cerevisiae.
ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae.
An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation.
Crystal structure of the ATPase domain of translation initiation factor 4A from Saccharomyces cerevisiae -- the prototype of the DEAD box protein family.
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase.
Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase.
Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally.
Interaction of translation initiation factor eIF4G with eIF4A in the yeast Saccharomyces cerevisiae.
Requirement of the self-glucosylating initiator proteins Glg1p and Glg2p for glycogen accumulation in Saccharomyces cerevisiae.
Sequence of the genes TIF1 and TIF2 from Saccharomyces cerevisiae coding for a translation initiation factor.
The 66 kDa component of yeast SFI, stimulatory factor I, is hsp60.
The Q motif: a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis.
The Saccharomyces cerevisiae RPB4 gene is tightly linked to the TIF2 gene.
Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system.
Translation initiation factor 4A from Saccharomyces cerevisiae: analysis of residues conserved in the D-E-A-D family of RNA helicases.
Translation initiation factor-dependent extracts from Saccharomyces cerevisiae.
Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA.
Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36.
Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene.
The ESS1 prolyl isomerase and its suppressor BYE1 interact with RNA pol II to inhibit transcription elongation in Saccharomyces cerevisiae.
Identification of nucleases and phosphatases by direct biochemical screen of the Saccharomyces cerevisiae proteome.
Structure and activity of the metal-independent fructose-1,6-bisphosphatase YK23 from Saccharomyces cerevisiae.
Characterization of a yeast mitochondrial ribosomal protein structurally related to the mammalian 68-kDa high affinity laminin receptor.
Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin.
Haa1, a protein homologous to the copper-regulated transcription factor ace1, is a novel transcriptional activator.
Variations of the C2H2 zinc finger motif in the yeast genome and classification of yeast zinc finger proteins.
High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing.
Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae.
The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames.
The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme.
Identification and characterization of five new subunits of TRAPP.
Mutants in trs120 disrupt traffic from the early endosome to the late Golgi.
Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae.
Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae.
SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes.
Identification of the essential EPE1 gene involved in retention of secreted proteins on the cell surface of Saccharomyces cerevisiae cells.
Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition.
Comparative spatial localization of protein-A-tagged and authentic yeast nuclear pore complex proteins by immunogold electron microscopy.
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
In situ analysis of spatial relationships between proteins of the nuclear pore complex.
In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p.
Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes.
Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p.
Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p.
Nup192p is a conserved nucleoporin with a preferential location at the inner site of the nuclear membrane.
Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96.
The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport.
The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex.
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction.
Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae.
The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes.
A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters.
Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage.
Gene recruitment of the activated INO1 locus to the nuclear membrane.
Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid.
The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper.
A transmembrane protein with a cdc2+/CDC28-related kinase activity is required for signaling from the ER to the nucleus.
IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae.
Oligomerization and phosphorylation of the Ire1p kinase during intracellular signaling from the endoplasmic reticulum to the nucleus.
The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response.
The unfolded protein response pathway in Saccharomyces cerevisiae. Oligomerization and trans-phosphorylation of Ire1p (Ern1p) are required for kinase activation.
A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts.
Activation of the cyclin-dependent kinase CTDK-I requires the heterodimerization of two unstable subunits.
Budding yeast CTDK-I is required for DNA damage-induced transcription.
C-terminal repeat domain kinase I phosphorylates Ser2 and Ser5 of RNA polymerase II C-terminal domain repeats.
CTD kinase I is involved in RNA polymerase I transcription.
CTD kinase I is required for the integrity of the rDNA tandem array.
Glucose deprivation mediates interaction between CTDK-I and Snf1 in Saccharomyces cerevisiae.
Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I.
The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex.
Bacterial overexpression of putative yeast mitochondrial transport proteins.
Biogenesis of the dicarboxylate carrier (DIC): translocation across the mitochondrial outer membrane and subsequent release from the TOM channel are membrane potential-independent.
Characterization, purification and properties of the yeast mitochondrial dicarboxylate carrier (Saccharomyces cerevisiae).
Identification of a novel gene encoding the yeast mitochondrial dicarboxylate transport protein via overexpression, purification, and characterization of its protein product.
The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase.
The mitochondrial dicarboxylate carrier is essential for the growth of Saccharomyces cerevisiae on ethanol or acetate as the sole carbon source.
A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export.
A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control.
Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast.
Arginine transcriptional response does not require inositol phosphate synthesis.
Characterization of two genes, ARGRI and ARGRIII required for specific regulation of arginine metabolism in yeast.
Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity.
Inositol polyphosphate kinase activity of Arg82/ArgRIII is not required for the regulation of the arginine metabolism in yeast.
Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae.
Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity.
Inositol trisphosphate metabolism in Saccharomyces cerevisiae: identification, purification and properties of inositol 1,4,5-trisphosphate 6-kinase.
Molecular definition of a novel inositol polyphosphate metabolic pathway initiated by inositol 1,4,5-trisphosphate 3-kinase activity in Saccharomyces cerevisiae.
Pleiotropic function of ArgRIIIp (Arg82p), one of the regulators of arginine metabolism in Saccharomyces cerevisiae. Role in expression of cell-type-specific genes.
Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability.
Regulation of arginine metabolism in Saccharomyces cerevisiae: expression of the three ARGR regulatory genes and cellular localization of their products.
Regulation of chromatin remodeling by inositol polyphosphates.
Synthesis of diphosphoinositol pentakisphosphate by a newly identified family of higher inositol polyphosphate kinases.
The transcriptional regulator, Arg82, is a hybrid kinase with both monophosphoinositol and diphosphoinositol polyphosphate synthase activity.
[PSI+] prion propagation is controlled by inositol polyphosphates.
Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51.
A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest.
Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast.
Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF.
The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene.
SWH1 from yeast encodes a candidate nuclear factor containing ankyrin repeats and showing homology to mammalian oxysterol-binding protein.
The ethanol-inducible YAT1 gene from yeast encodes a presumptive mitochondrial outer carnitine acetyltransferase.
Crucial role of conserved cysteine residues in the assembly of two iron-sulfur clusters on the CIA protein Nar1.
Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase.
Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis.
The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly.
The hydrogenase-like Nar1p is essential for maturation of cytosolic and nuclear iron-sulphur proteins.
COQ2 is a candidate for the structural gene encoding para-hydroxybenzoate:polyprenyltransferase.
Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase.
Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis.
The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity.
Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism.
The ZRT2 gene encodes the low affinity zinc transporter in Saccharomyces cerevisiae.
Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis.
Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast.
The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.
Para-aminobenzoate synthase gene of Saccharomyces cerevisiae encodes a bifunctional enzyme.
A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity.
A localized GTPase exchange factor, Bud5, determines the orientation of division axes in yeast.
Functional cloning of BUD5, a CDC25-related gene from S. cerevisiae that can suppress a dominant-negative RAS2 mutant.
Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast.
Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae.
The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames.
Yeast BUD5, encoding a putative GDP-GTP exchange factor, is necessary for bud site selection and interacts with bud formation gene BEM1.
A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes.
Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae.
Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex.
The S. cerevisiae CLU1 and D. discoideum cluA genes are functional homologues that influence mitochondrial morphology and distribution.
DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae.
Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration.
Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair.
Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae.
Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.
Multiple endonucleases function to repair covalent topoisomerase I complexes in Saccharomyces cerevisiae.
Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiae.
Phosphorylation of Slx4 by Mec1 and Tel1 regulates the single-strand annealing mode of DNA repair in budding yeast.
Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae.
Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs.
Slx1-Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1-Top3.
Slx4 becomes phosphorylated after DNA damage in a Mec1/Tel1-dependent manner and is required for repair of DNA alkylation damage.
Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4.
A nuclear gene essential for mitochondrial replication suppresses a defect of mitochondrial transcription in Saccharomyces cerevisiae.
A point mutation in the core subunit gene of yeast mitochondrial RNA polymerase is suppressed by a high level of specificity factor MTF1.
Assignment of a yeast protein necessary for mitochondrial transcription initiation.
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription.
The yeast mitochondrial RNA polymerase specificity factor, MTF1, is similar to bacterial sigma factors.
Identification of the yeast mitochondrial transporter for oxaloacetate and sulfate.
Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones.
An essential gene pair in Saccharomyces cerevisiae with a potential role in mating.
Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes.
Ssf1p prevents premature processing of an early pre-60S ribosomal particle.
YNT20, a bypass suppressor of yme1 yme2, encodes a putative 3'-5' exonuclease localized in mitochondria of Saccharomyces cerevisiae.
The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10.
DNA.RNA helicase activity of RAD3 protein of Saccharomyces cerevisiae.
Effects of multiple yeast rad3 mutant alleles on UV sensitivity, mutability, and mitotic recombination.
Mutation of lysine-48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP.
RAD3 gene of Saccharomyces cerevisiae: nucleotide sequence of wild-type and mutant alleles, transcript mapping, and aspects of gene regulation.
Structure of a complete mediator-RNA polymerase II pre-initiation complex.
Structure of an RNA polymerase II preinitiation complex.
The DNA repair helicases XPD and FancJ have essential iron-sulfur domains.
The RAD3 gene is a member of the DEAH family RNA helicase-like protein.
The Rad3 protein from Saccharomyces cerevisiae: a DNA and DNA:RNA helicase with putative RNA helicase activity.
The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae: a potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region.
Complete sequence of a eukaryotic regulatory gene.
Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha.
Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae.
Coa2 is an assembly factor for yeast cytochrome C oxidase biogenesis that facilitates the maturation of cox1.
SHY1, the yeast homolog of the mammalian SURF-1 gene, encodes a mitochondrial protein required for respiration.
Shy1p is necessary for full expression of mitochondrial COX1 in the yeast model of Leigh's syndrome.
The sequence of a 23.4 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals CLB6, SPT6, RP28A and NUP57 genes, a Ty3 element and 11 new open reading frames.
An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p.
Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification.
Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene.
Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex.
An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes.
The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus.
Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion.
Vps51p links the VFT complex to the SNARE Tlg1p.
Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p.
Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi.
Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes.
Structure and transcriptional control of the Saccharomyces cerevisiae POX1 gene encoding acyl-coenzyme A oxidase.
The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames.
Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.
Mutual antagonism of TOR and calcineurin signaling.
Slm1 and slm2 are novel substrates of the calcineurin phosphatase required for heat stress-induced endocytosis of the yeast uracil permease.
The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2.
20S RNA narnavirus defies the antiviral activity of SKI1/XRN1 in Saccharomyces cerevisiae.
A multifunctional exonuclease from vegetative Schizosaccharomyces pombe cells exhibiting in vitro strand exchange activity.
A role for KEM1 at the START of the cell cycle in Saccharomyces cerevisiae.
A role of Sep1 (= Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae.
Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis.
An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.
Analysis of P-body assembly in Saccharomyces cerevisiae.
Characterization of the XRN1 gene encoding a 5'-->3' exoribonuclease: sequence data and analysis of disparate protein and mRNA levels of gene-disrupted yeast cells.
Characterization of the interaction of Saccharomyces cerevisiae strand exchange protein 1 with DNA.
Cloning and characterization of DST2, the gene for DNA strand transfer protein beta from Saccharomyces cerevisiae.
Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation.
Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1.
Deletion of OSH3 gene confers resistance against ISP-1 in Saccharomyces cerevisiae.
Determinants of Rbp1p localization in specific cytoplasmic mRNA-processing foci, P-bodies.
Disruption of the gene XRN1, coding for a 5'-->3' exoribonuclease, restricts yeast cell growth.
Gene disruption of a G4-DNA-dependent nuclease in yeast leads to cellular senescence and telomere shortening.
Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast.
Initiation-mediated mRNA decay in yeast affects heat-shock mRNAs, and works through decapping and 5'-to-3' hydrolysis.
KEM1 is involved in filamentous growth of Saccharomyces cerevisiae.
Molecular and genetic analysis of the gene encoding the Saccharomyces cerevisiae strand exchange protein Sep1.
Multiple RNA surveillance pathways limit aberrant expression of iron uptake mRNAs and prevent iron toxicity in S. cerevisiae.
Mutational analysis of exoribonuclease I from Saccharomyces cerevisiae.
Posttranscriptional regulation of the karyogamy gene by Kem1p/Xrn1p exoribonuclease and Rok1p RNA helicase of Saccharomyces cerevisiae.
Processing of the precursors to small nucleolar RNAs and rRNAs requires common components.
RNase MRP cleaves the CLB2 mRNA to promote cell cycle progression: novel method of mRNA degradation.
Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively.
Regulation and intracellular localization of Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multifunctional exonuclease.
Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation.
Respiratory deficiency mediates the regulation of CHO1-encoded phosphatidylserine synthase by mRNA stability in Saccharomyces cerevisiae.
S. cerevisiae Vts1p induces deadenylation-dependent transcript degradation and interacts with the Ccr4p-Pop2p-Not deadenylase complex.
Sodium-induced GCN4 expression controls the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate.
Strand exchange protein 1 from Saccharomyces cerevisiae. A novel multifunctional protein that contains DNA strand exchange and exonuclease activities.
Suppression of viral RNA recombination by a host exoribonuclease.
Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.
Telomere length regulation and telomeric chromatin require the nonsense-mediated mRNA decay pathway.
The Cth2 ARE-binding protein recruits the Dhh1 helicase to promote the decay of succinate dehydrogenase SDH4 mRNA in response to iron deficiency.
The DEAD-box RNA helicase Ded1p affects and accumulates in Saccharomyces cerevisiae P-bodies.
The Sep1 strand exchange protein from Saccharomyces cerevisiae promotes a paranemic joint between homologous DNA molecules.
The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides.
The activity of the Saccharomyces cerevisiae strand exchange protein 1 intrinsic exonuclease during joint molecule formation.
The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'->3' exonucleases.
The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5'-end processing of S. cerevisiae box C/D snoRNAs.
The sensitivity of yeast mutants to oleic acid implicates the peroxisome and other processes in membrane function.
The sep1 mutant of Saccharomyces cerevisiae arrests in pachytene and is deficient in meiotic recombination.
The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes.
The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure.
Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes.
Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs.
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components.
Yeast transcripts cleaved by an internal ribozyme provide new insight into the role of the cap and poly(A) tail in translation and mRNA decay.
kem mutations affect nuclear fusion in Saccharomyces cerevisiae.
rar mutations which increase artificial chromosome stability in Saccharomyces cerevisiae identify transcription and recombination proteins.
Binding of the replication terminator protein Fob1p to the Ter sites of yeast causes polar fork arrest.
The Reb1-homologue Ydr026c/Nsi1 is required for efficient RNA polymerase I termination in yeast.
The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein which is sorted by the HDEL retrieval system.
Use1p is a yeast SNARE protein required for retrograde traffic to the ER.
Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria.
Cox25 teams up with Mss51, Ssc1, and Cox14 to regulate mitochondrial cytochrome c oxidase subunit 1 expression and assembly in Saccharomyces cerevisiae.
Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae.
Synergy between trehalose and Hsp104 for thermotolerance in Saccharomyces cerevisiae.
Sequence of peptides from Saccharomyces cerevisiae glutamine synthetase. N-terminal peptide and ATP-binding domain.
Sequence of the GLN1 gene of Saccharomyces cerevisiae: role of the upstream region in regulation of glutamine synthetase expression.
Cdc48 and Ufd3, new partners of the ubiquitin protease Ubp3, are required for ribophagy.
Molecular basis for bre5 cofactor recognition by the ubp3 deubiquitylating enzyme.
Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23.
Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p.
Ady3p links spindle pole body function to spore wall synthesis in Saccharomyces cerevisiae.
Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae.
Cnm67p is a spacer protein of the Saccharomyces cerevisiae spindle pole body outer plaque.
Prospore membrane formation linked to the leading edge protein (LEP) coat assembly.
Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis.
Saccharomyces cerevisiae cells with defective spindle pole body outer plaques accomplish nuclear migration via half-bridge-organized microtubules.
YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae.
Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing.
Characterization of two genes required for the position-effect control of yeast mating-type genes.
Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci.
Importance of the sir3 N terminus and its acetylation for yeast transcriptional silencing.
Structure of the Sir3 protein bromo adjacent homology (BAH) domain from S. cerevisiae at 1.95 A resolution.
The molecular biology of the SIR proteins.
Functional comparison of citrate synthase isoforms from S. cerevisiae.
Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.
Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae.
Ptc7p Dephosphorylates Select Mitochondrial Proteins to Enhance Metabolic Function.
Chaperone-mediated pathway of proteasome regulatory particle assembly.
Hexameric assembly of the proteasomal ATPases is templated through their C termini.
Hsm3/S5b participates in the assembly pathway of the 19S regulatory particle of the proteasome.
Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base.
Requirement of HSM3 gene for spontaneous mutagenesis in Saccharomyces cerevisiae.
The yeast HSM3 gene acts in one of the mismatch repair pathways.
The yeast HSM3 gene is involved in DNA mismatch repair in slowly dividing cells.
The yeast HSM3 gene is not involved in DNA mismatch repair in rapidly dividing cells.
Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock.
Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.
Incomplete arrest in the outer membrane sorts NADH-cytochrome b5 reductase to two different submitochondrial compartments.
Mitochondrial NADH-cytochrome b(5) reductase plays a crucial role in the reduction of D-erythroascorbyl free radical in Saccharomyces cerevisiae.
The yeast mitochondrial intermembrane space: purification and analysis of two distinct fractions.
Genomic screen for vacuolar protein sorting genes in Saccharomyces cerevisiae.
Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI.
Structure and function of MRP20 and MRP49, the nuclear genes for two proteins of the 54 S subunit of the yeast mitochondrial ribosome.
Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.
A general amphipathic alpha-helical motif for sensing membrane curvature.
A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein.
A phosphatidylinositol-4-phosphate powered exchange mechanism to create a lipid gradient between membranes.
Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex.
Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.
Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers.
Oxysterol-binding protein homologs mediate sterol transport from the endoplasmic reticulum to mitochondria in yeast.
Phosphatidylserine transport by ORP/Osh proteins is driven by phosphatidylinositol 4-phosphate.
Structural mechanism for sterol sensing and transport by OSBP-related proteins.
Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
A TOG protein confers tension sensitivity to kinetochore-microtubule attachments.
A TOG:alphabeta-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase.
A tethered delivery mechanism explains the catalytic action of a microtubule polymerase.
Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.
Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.
Stu2 promotes mitotic spindle elongation in anaphase.
Stu2, the budding yeast XMAP215/Dis1 homolog, promotes assembly of yeast microtubules by increasing growth rate and decreasing catastrophe frequency.
Stu2p binds tubulin and undergoes an open-to-closed conformational change.
Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body.
The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly.
A conserved domain within Arc1p delivers tRNA to aminoacyl-tRNA synthetases.
Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs.
Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1.
Cytoplasmic methionyl-tRNA synthetase from Bakers' yeast. A monomer with a post-translationally modified N-terminus.
Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.
Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase.
Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes.
The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p.
The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases.
Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes.
Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.
The Saccharomyces cerevisiae aldose reductase is implied in the metabolism of methylglyoxal in response to stress conditions.
The structure and function of yeast xylose (aldose) reductases.
Three genes whose expression is induced by stress in Saccharomyces cerevisiae.
Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal.
Intramitochondrial functions regulate nonmitochondrial citrate synthase (CIT2) expression in Saccharomyces cerevisiae.
Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.
Saccharomyces cerevisiae contains two functional citrate synthase genes.
The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae.
The ubiquitin ligase SCF(Ucc1) acts as a metabolic switch for the glyoxylate cycle.
A membrane-inserted structural model of the yeast mitofusin Fzo1.
A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro.
A mutation associated with CMT2A neuropathy causes defects in Fzo1 GTP hydrolysis, ubiquitylation, and protein turnover.
Acetyl-L-carnitine protects yeast cells from apoptosis and aging and inhibits mitochondrial fission.
Connection of the mitochondrial outer and inner membranes by Fzo1 is critical for organellar fusion.
Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape.
Doa1 targets ubiquitinated substrates for mitochondria-associated degradation.
Domain interactions within Fzo1 oligomers are essential for mitochondrial fusion.
Fzo1p is a mitochondrial outer membrane protein essential for the biogenesis of functional mitochondria in Saccharomyces cerevisiae.
Glucose levels regulate the nucleo-mitochondrial distribution of Mig2.
Instability of the mitofusin Fzo1 regulates mitochondrial morphology during the mating response of the yeast Saccharomyces cerevisiae.
Mdm36 is a mitochondrial fission-promoting protein in Saccharomyces cerevisiae.
Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane.
Mitochondrial fusion in yeast requires the transmembrane GTPase Fzo1p.
Mitochondrial fusion intermediates revealed in vitro.
Regulation of mitochondrial fusion by the F-box protein Mdm30 involves proteasome-independent turnover of Fzo1.
Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion.
Two deubiquitylases act on mitofusin and regulate mitochondrial fusion along independent pathways.
Ubiquitin-proteasome-dependent degradation of a mitofusin, a critical regulator of mitochondrial fusion.
Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion.
Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion.
A novel inhibitor of cap-dependent translation initiation in yeast: p20 competes with eIF4G for binding to eIF4E.
Characterization of the in vivo phosphorylation sites of the mRNA.cap-binding complex proteins eukaryotic initiation factor-4E and p20 in Saccharomyces cerevisiae.
Cooperative modulation by eIF4G of eIF4E-binding to the mRNA 5' cap in yeast involves a site partially shared by p20.
Eap1p, a novel eukaryotic translation initiation factor 4E-associated protein in Saccharomyces cerevisiae.
Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1.
Interactions of the eIF-4F subunits in the yeast Saccharomyces cerevisiae.
Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function.
Modulation of eukaryotic mRNA stability via the cap-binding translation complex eIF4F.
Nucleotide sequence of the gene encoding a 20 kDa protein associated with the cap binding protein eIF-4E from Saccharomyces cerevisiae.
Regulation of translation initiation by the yeast eIF4E binding proteins is required for the pseudohyphal response.
SnR31, snR32, and snR33: three novel, non-essential snRNAs from Saccharomyces cerevisiae.
Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress.
Cytosolic thioredoxin peroxidase I and II are important defenses of yeast against organic hydroperoxide insult: catalases and peroxiredoxins cooperate in the decomposition of H2O2 by yeast.
Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae.
Purification and characterization of a second type thioredoxin peroxidase (type II TPx) from Saccharomyces cerevisiae.
Structure of TSA2 reveals novel features of the active-site loop of peroxiredoxins.
Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function.
Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired.
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins.
Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1.
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.
A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg).
Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiae.
Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae.
DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae.
Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo.
Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2.
Quality control of inner nuclear membrane proteins by the Asi complex.
Suppressors of ssy1 and ptr3 null mutations define novel amino acid sensor-independent genes in Saccharomyces cerevisiae.
Domains of the SFL1 protein of yeasts are homologous to Myc oncoproteins or yeast heat-shock transcription factor.
Essential role of Swp73p in the function of yeast Swi/Snf complex.
The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles.
An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase.
Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes.
Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae.
Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames.
The sequence of a 12 kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato.
A novel protein modification pathway related to the ubiquitin system.
Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCFCdc4 complex.
Different secondary structure elements as scaffolds for protein folding transition states of two homologous four-helix bundles.
Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein.
Saccharomyces carlsbergensis contains two functional genes encoding the acyl-CoA binding protein, one similar to the ACB1 gene from S. cerevisiae and one identical to the ACB1 gene from S. monacensis.
An essential role for Clp1 in assembly of polyadenylation complex CF IA and Pol II transcription termination.
Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I.
Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo.
Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends.
Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology.
Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor.
The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation.
The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site.
The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3'-end formation.
60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle.
Composition and functional characterization of yeast 66S ribosome assembly intermediates.
Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0.
Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae.
Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover.
The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein.
Mitochondrial ribosomes of yeast: isolation of individual proteins and N-terminal sequencing.
Molecular cloning of the nuclear gene for mitochondrial ribosomal protein YmL31 from Saccharomyces cerevisiae.
Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p.
Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis.
Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA.
A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae.
The yeast exosome functions as a macromolecular cage to channel RNA substrates for degradation.
Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames.
Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV.
Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis.
Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast.
Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.
Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway.
Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae.
Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall.
Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae.
Molecular cloning of CWP1: a gene encoding a Saccharomyces cerevisiae cell wall protein solubilized with Rarobacter faecitabidus protease I.
Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked beta-1,3-/beta-1,6-glucan heteropolymer.
Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast.
Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae.
The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants.
Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae.
Cuz1/Ynl155w, a zinc-dependent ubiquitin-binding protein, protects cells from metalloid-induced proteotoxicity.
Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast.
Isolation of active yeast telomerase protein Est3p and investigation of its dimerization in vitro.
Programmed translational frameshifting in a gene required for yeast telomere replication.
Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo.
Saccharomyces cerevisiae telomerase subunit Est3p binds DNA and RNA and stimulates unwinding of RNA/DNA heteroduplexes.
Senescence mutants of Saccharomyces cerevisiae with a defect in telomere replication identify three additional EST genes.
Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases alpha and delta.
The Est3 protein associates with yeast telomerase through an OB-fold domain.
The Est3 protein is a subunit of yeast telomerase.
Three ever shorter telomere (EST) genes are dispensable for in vitro yeast telomerase activity.
Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex.
Identification of novel protein-protein interactions at the cytosolic surface of the Sec63 complex in the yeast ER membrane.
Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum.
Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p.
SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum.
Functional analysis of free methionine-R-sulfoxide reductase from Saccharomyces cerevisiae.
Structure of the GAF domain, a ubiquitous signaling motif and a new class of cyclic GMP receptor.
Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins.
Amyloid-beta peptide induces mitochondrial dysfunction by inhibition of preprotein maturation.
Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae.
Purification and characterization of protease M, a yeast mitochondrial nucleotide-stimulated metal protease: Its identification as CYM1 gene product, a mitochondrial presequence peptidase.
Role of the novel metallopeptidase Mop112 and saccharolysin for the complete degradation of proteins residing in different subcompartments of mitochondria.
Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
The Saccharomyces cerevisiae MTS1 gene encodes a putative RNA-binding protein involved in mitochondrial protein targeting.
A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.
Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding.
Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30) complex.
Functional analysis of Met4, a yeast transcriptional activator responsive to S-adenosylmethionine.
Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p.
MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae.
Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats.
The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs.
The general amino acid control regulates MET4, which encodes a methionine-pathway-specific transcriptional activator of Saccharomyces cerevisiae.
The yeast ubiquitin ligase SCFMet30 regulates heavy metal response.
Identification of the mitochondrial GTP/GDP transporter in Saccharomyces cerevisiae.
Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast.
SHM1: a multicopy suppressor of a temperature-sensitive null mutation in the HMG1-like abf2 gene.
The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein.
ER network formation requires a balance of the dynamin-like GTPase Sey1p and the Lunapark family member Lnp1p.
Nucleolar protein Nop12p participates in synthesis of 25S rRNA in Saccharomyces cerevisiae.
CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiae.
Coenzyme Q supplementation or over-expression of the yeast Coq8 putative kinase stabilizes multi-subunit Coq polypeptide complexes in yeast coq null mutants.
Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial targeting of the Escherichia coli UbiF polypeptide: two functions of yeast Coq7 polypeptide in coenzyme Q biosynthesis.
Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis.
Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex.
The COQ7 gene encodes a protein in Saccharomyces cerevisiae necessary for ubiquinone biosynthesis.
The phosphatase Ptc7 induces coenzyme Q biosynthesis by activating the hydroxylase Coq7 in yeast.
Yeast Clk-1 homologue (Coq7/Cat5) is a mitochondrial protein in coenzyme Q synthesis.
Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants.
The upstream activation site of a Ty2 element of yeast is necessary but not sufficient to promote maximal transcription of the element.
A sequence resembling a peroxisomal targeting sequence directs the interaction between the tetratricopeptide repeats of Ssn6 and the homeodomain of alpha 2.
Cloning and characterization of the CYC8 gene mediating glucose repression in yeast.
Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1.
Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo.
Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme.
Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae.
Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins.
Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein.
Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress.
Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein.
SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-Tup1p repression in Saccharomyces cerevisiae.
Ssn6-Tup1 interacts with class I histone deacetylases required for repression.
The Cyc8 (Ssn6)-Tup1 corepressor complex is composed of one Cyc8 and four Tup1 subunits.
The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor.
The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2.
The yeast global transcriptional co-repressor protein Cyc8 can propagate as a prion.
Tup1-Ssn6 interacts with multiple class I histone deacetylases in vivo.
A quantitative characterization of the yeast heterotrimeric G protein cycle.
Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome.
Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling.
Biochemical analysis of yeast G(alpha) mutants that enhance adaptation to pheromone.
Characterization of Saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for G protein alpha subunit deacylation in vivo.
Constitutive mutants in the yeast pheromone response: ordered function of the gene products.
Differential regulation of G protein alpha subunit trafficking by mono- and polyubiquitination.
Direct identification of a G protein ubiquitination site by mass spectrometry.
Dominant-negative inhibition of pheromone receptor signaling by a single point mutation in the G protein alpha subunit.
Dual lipid modification motifs in G(alpha) and G(gamma) subunits are required for full activity of the pheromone response pathway in Saccharomyces cerevisiae.
Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase.
Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors.
G protein mutations that alter the pheromone response in Saccharomyces cerevisiae.
GPA1Val-50 mutation in the mating-factor signaling pathway in Saccharomyces cerevisiae.
Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae.
Interactions among the subunits of the G protein involved in Saccharomyces cerevisiae mating.
Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling.
Mutations in the guanine nucleotide-binding domains of a yeast G alpha protein confer a constitutive or uninducible state to the pheromone response pathway.
N-myristoylation is required for function of the pheromone-responsive G alpha protein of yeast: conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase.
Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins.
Partial constitutive activation of pheromone responses by a palmitoylation-site mutant of a G protein alpha subunit in yeast.
Pheromone action regulates G-protein alpha-subunit myristoylation in the yeast Saccharomyces cerevisiae.
Regulation of MAPK function by direct interaction with the mating-specific G(alpha) in yeast.
Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast.
Regulation of the yeast pheromone response pathway by G protein subunits.
Second site suppressor mutations of a GTPase-deficient G-protein alpha-subunit.
Selective uncoupling of RGS action by a single point mutation in the G protein alpha-subunit.
Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeast.
Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: Expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit).
Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway.
Suppression of a dominant G-protein beta-subunit mutation in yeast by G alpha protein expression.
Switch-domain mutations in the Saccharomyces cerevisiae G protein alpha-subunit Gpa1p identify a receptor subtype-biased mating defect.
The C terminus of the Saccharomyces cerevisiae alpha-factor receptor contributes to the formation of preactivation complexes with its cognate G protein.
The GTP hydrolysis defect of the Saccharomyces cerevisiae mutant G-protein Gpa1(G50V).
The carboxyl terminus of Scg1, the G alpha subunit involved in yeast mating, is implicated in interactions with the pheromone receptors.
The mating-specific G(alpha) protein of Saccharomyces cerevisiae downregulates the mating signal by a mechanism that is dependent on pheromone and independent of G(beta)(gamma) sequestration.
The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160.
The yeast G-protein homolog is involved in the mating pheromone signal transduction system.
The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway.
The yeast pheromone-responsive G alpha protein stimulates recovery from chronic pheromone treatment by two mechanisms that are activated at distinct levels of stimulus.
An essential role for a phospholipid transfer protein in yeast Golgi function.
Biophysical parameters of the Sec14 phospholipid exchange cycle - Effect of lipid packing in membranes.
Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein.
Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14.
Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein.
Phospholipid transfer protein Sec14 is required for trafficking from endosomes and regulates distinct trans-Golgi export pathways.
Target identification and mechanism of action of picolinamide and benzamide chemotypes with antifungal properties.
The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex.
The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity.
The chemistry of phospholipid binding by the Saccharomyces cerevisiae phosphatidylinositol transfer protein Sec14p as determined by EPR spectroscopy.
The gene encoding the phosphatidylinositol transfer protein is essential for cell growth.
Yeast Sec14p deficient in phosphatidylinositol transfer activity is functional in vivo.
An early function during transcription for the yeast mRNA export factor Dbp5p/Rat8p suggested by its genetic and physical interactions with transcription factor IIH components.
The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames.
A conserved family of proteins facilitates nascent lipid droplet budding from the ER.
Architecture of lipid droplets in endoplasmic reticulum is determined by phospholipid intrinsic curvature.
Fat storage-inducing transmembrane (FIT or FITM) proteins are related to lipid phosphatase/phosphotransferase enzymes.
A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor.
Yeast single copy gene URP1 is a homolog of rat ribosomal protein gene L21.
A cdc-like autolytic Saccharomyces cerevisiae mutant altered in budding site selection is complemented by SPO12, a sporulation gene.
A novel functional domain of Cdc15 kinase is required for its interaction with Tem1 GTPase in Saccharomyces cerevisiae.
Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis.
CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain.
Cdc14 activates cdc15 to promote mitotic exit in budding yeast.
Cdc15 integrates Tem1 GTPase-mediated spatial signals with Polo kinase-mediated temporal cues to activate mitotic exit.
Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae.
Cell cycle phosphorylation of mitotic exit network (MEN) proteins.
Cell cycle-dependent phosphorylation of Rad53 kinase by Cdc5 and Cdc28 modulates checkpoint adaptation.
Chromosome condensation and sister chromatid pairing in budding yeast.
Chromosome separation and exit from mitosis in budding yeast: dependence on growth revealed by cAMP-mediated inhibition.
Control of the mitotic exit network during meiosis.
Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.
Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14.
Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast.
Independent modulation of the kinase and polo-box activities of Cdc5 protein unravels unique roles in the maintenance of genome stability.
Measurement and modeling of transcriptional noise in the cell cycle regulatory network.
Mitotic exit regulation through distinct domains within the protein kinase Cdc15.
Mutual regulation of cyclin-dependent kinase and the mitotic exit network.
NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA.
Order of function of the budding-yeast mitotic exit-network proteins Tem1, Cdc15, Mob1, Dbf2, and Cdc5.
Phosphorylation and spindle pole body localization of the Cdc15p mitotic regulatory protein kinase in budding yeast.
Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex.
Regulation of the mitotic exit protein kinases Cdc15 and Dbf2.
Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation.
Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast.
The FEAR protein Slk19 restricts Cdc14 phosphatase to the nucleus until the end of anaphase, regulating its participation in mitotic exit in Saccharomyces cerevisiae.
The budding yeast Cdc15 localizes to the spindle pole body in a cell-cycle-dependent manner.
The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit.
Unrestrained spindle elongation during recovery from spindle checkpoint activation in cdc15-2 cells results in mis-segregation of chromosomes.
The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae.
Identification of a nuclear gene (FMC1) required for the assembly/stability of yeast mitochondrial F(1)-ATPase in heat stress conditions.
Identification of factors regulating poly(A) tail synthesis and maturation.
Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation.
REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation.
RNA binding analysis of yeast REF2 and its two-hybrid interaction with a new gene product, FIR1.
Characterization of the glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons.
Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis.
Nucleotide sequence of the yeast SUC2 gene for invertase.
Secretion-defective mutations in the signal sequence for Saccharomyces cerevisiae invertase.
The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.
[The effects of upstream region of SUC2 gene on its expression].
A DNA integrity network in the yeast Saccharomyces cerevisiae.
A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.
Taking it off: regulation of H3 K56 acetylation by Hst3 and Hst4.
The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability.
The sirtuins Hst3 and Hst4p preserve genome integrity by controlling histone H3 lysine 56 deacetylation.
LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth.
A genomic screen for yeast vacuolar membrane ATPase mutants.
Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae.
Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway.
TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation.
Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p.
KAR3, a kinesin-related gene required for yeast nuclear fusion.
Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations.
The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae.
X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution.
Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends.
Reverse methionine biosynthesis from S-adenosylmethionine in eukaryotic cells.
Identification of an additional gene required for eukaryotic nonsense mRNA turnover.
Potential DNA-binding domains in the RAD18 gene product of Saccharomyces cerevisiae.
RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO.
Rad18/Rad5/Mms2-mediated polyubiquitination of PCNA is implicated in replication completion during replication stress.
Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae.
Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites.
The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence.
The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways.
The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.
The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiae.
Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair.
Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities.
A mutation in the MSH6 subunit of the Saccharomyces cerevisiae MSH2-MSH6 complex disrupts mismatch recognition.
ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes.
Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system.
Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.
Analysis of yeast MSH2-MSH6 suggests that the initiation of mismatch repair can be separated into discrete steps.
Asymmetric recognition of DNA local distortion. Structure-based functional studies of eukaryotic Msh2-Msh6.
Biochemical basis for dominant mutations in the Saccharomyces cerevisiae MSH6 gene.
Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.
Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.
Cadmium inhibits the functions of eukaryotic MutS complexes.
Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.
Contribution of Msh2 and Msh6 subunits to the asymmetric ATPase and DNA mismatch binding activities of Saccharomyces cerevisiae Msh2-Msh6 mismatch repair protein.
Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis.
Dominant Saccharomyces cerevisiae msh6 mutations cause increased mispair binding and decreased dissociation from mispairs by Msh2-Msh6 in the presence of ATP.
Evidence for sequential action of two ATPase active sites in yeast Msh2-Msh6.
Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes.
Germ-line msh6 mutations in colorectal cancer families.
Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2(ATP)-Msh6(ATP) state capable of hydrolysis-independent movement along DNA.
MSH-MLH complexes formed at a DNA mismatch are disrupted by the PCNA sliding clamp.
MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae.
MSH6, a Saccharomyces cerevisiae protein that binds to mismatches as a heterodimer with MSH2.
Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.
Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.
Multiple functions for the N-terminal region of Msh6.
Novel dominant mutations in Saccharomyces cerevisiae MSH6.
Proliferating cell nuclear antigen and Msh2p-Msh6p interact to form an active mispair recognition complex.
Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.
Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition.
Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes.
Saccharomyces cerevisiae Msh2p and Msh6p ATPase activities are both required during mismatch repair.
Specialized mismatch repair function of Glu339 in the Phe-X-Glu motif of yeast Msh6.
The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.
The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs.
Transfer of the MSH2.MSH6 complex from proliferating cell nuclear antigen to mispaired bases in DNA.
A novel programmed frameshift expresses the POL3 gene of retrotransposon Ty3 of yeast: frameshifting without tRNA slippage.
A yeast sigma composite element, TY3, has properties of a retrotransposon.
Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein.
Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro.
Investigation by atomic force microscopy of the structure of Ty3 retrotransposon particles.
Proteolytic processing of Ty3 proteins is required for transposition.
Ten-kilodalton domain in Ty3 Gag3-Pol3p between PR and RT is dispensable for Ty3 transposition.
The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins.
Ty3 GAG3 and POL3 genes encode the components of intracellular particles.
Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene.
Characterization of two forms of asparaginase in Saccharomyces cerevisiae.
Nitrogen catabolite repression of asparaginase II in Saccharomyces cerevisiae.
Reactions of asparaginase II of Saccharomyces cerevisiae. A mechanistic analysis of hydrolysis and hydroxylaminolysis.
Characterization of human and yeast mitochondrial glycine carriers with implications for heme biosynthesis and anemia.
Glycine and folate ameliorate models of congenital sideroblastic anemia.
Mitochondrial ClpX activates a key enzyme for heme biosynthesis and erythropoiesis.
Mutations in mitochondrial carrier family gene SLC25A38 cause nonsyndromic autosomal recessive congenital sideroblastic anemia.
Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae.
Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.
The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts.
Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.
A proteomic strategy for gaining insights into protein sumoylation in yeast.
Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames.
Structural comparison of yeast ribosomal protein genes.
A new hyperrecombination mutation identifies a novel yeast gene, THP1, connecting transcription elongation with mitotic recombination.
Genome-wide nuclear morphology screen identifies novel genes involved in nuclear architecture and gene-silencing in Saccharomyces cerevisiae.
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase.
Identification of new fungal peroxisomal matrix proteins and revision of the PTS1 consensus.
Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides.
Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae. II. VAM7, a gene for regulating morphogenic assembly of the vacuoles.
Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames.
Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking.
Characterization of a Saccharomyces cerevisiae gene that encodes a mitochondrial phosphate transporter-like protein.
Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana.
A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein.
Expression, proteolytic analysis, reconstitution, and crystallization of the tau60/tau91 subcomplex of yeast TFIIIC.
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly.
Functional organization of the yeast proteome by systematic analysis of protein complexes.
SHR3: a novel component of the secretory pathway specifically required for localization of amino acid permeases in yeast.
Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7 and a tRNA for arginine.
Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2.
Telomere length as a quantitative trait: genome-wide survey and genetic mapping of telomere length-control genes in yeast.
Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.
COX23, a homologue of COX17, is required for cytochrome oxidase assembly.
Novel mitochondrial intermembrane space proteins as substrates of the MIA import pathway.
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm.
Screening for novel essential genes of Saccharomyces cerevisiae involved in protein secretion.
Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae.
The Saccharomyces cerevisiae SDA1 gene is required for actin cytoskeleton organization and cell cycle progression.
The Sda1 protein is required for passage through start.
The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects.
A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization.
Activation of the Arp2/3 complex by the actin filament binding protein Abp1p.
Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I.
In vivo analysis of the domains of yeast Rvs167p suggests Rvs167p function is mediated through multiple protein interactions.
Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics.
Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter functions.
Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis.
The sequence of a 13.5 kb DNA segment from the left arm of yeast chromosome XIV reveals MER1; RAP1; a new putative member of the DNA replication complex and a new putative serine/threonine phosphatase gene.
Control by sugar of Saccharomyces cerevisiae flocculation for industrial ethanol production.
Distribution of the flocculation protein, flop, at the cell surface during yeast growth: the availability of flop determines the flocculation level.
Localization of the dominant flocculation genes FLO5 and FLO8 of Saccharomyces cerevisiae.
Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family.
Yeast flocculation: what brewers should know.
Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease.
Site-directed mutagenesis of the yeast resolving enzyme Cce1 reveals catalytic residues and relationship with the intron-splicing factor Mrs1.
The structure-selectivity and sequence-preference of the junction-resolving enzyme CCE1 of Saccharomyces cerevisiae.
Ric1p and Rgp1p form a complex that catalyses nucleotide exchange on Ypt6p.
Ric1p and the Ypt6p GTPase function in a common pathway required for localization of trans-Golgi network membrane proteins.
An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane.
Assembly of the TOB complex of mitochondria.
Mas37p, a novel receptor subunit for protein import into mitochondria.
Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation.
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae.
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis.
Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis.
Solution structure of the yeast ubiquitin-like modifier protein Hub1.
SLI1 (YGR212W) is a major gene conferring resistance to the sphingolipid biosynthesis inhibitor ISP-1, and encodes an ISP-1 N-acetyltransferase in yeast.
STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae.
Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction.
Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM.
Targeted proteomic study of the cyclin-Cdk module.
The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae chromosome XI contains the UBI2 and MPL1 genes and three new open reading frames.
A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p.
A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel.
PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae.
Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways.
The Saccharomyces cerevisiae peroxisomal import receptor Pex5p is monoubiquitinated in wild type cells.
The peroxisomal importomer constitutes a large and highly dynamic pore.
The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL.
Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling.
Ubiquitination of the peroxisomal import receptor Pex5p.
Ubiquitination of the peroxisomal targeting signal type 1 receptor, Pex5p, suggests the presence of a quality control mechanism during peroxisomal matrix protein import.
Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae.
High-resolution crystal structure of S. cerevisiae Ypt51(DeltaC15)-GppNHp, a small GTP-binding protein involved in regulation of endocytosis.
Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast.
The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting.
Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition.
VPS21 controls entry of endocytosed and biosynthetic proteins into the yeast prevacuolar compartment.
VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins.
Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome.
Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport.
Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p.
The TFB4 subunit of yeast TFIIH is required for both nucleotide excision repair and RNA polymerase II transcription.
Divergent overlapping transcripts at the PET122 locus in Saccharomyces cerevisiae.
Molecular cloning and nucleotide sequence of the nuclear PET122 gene required for expression of the mitochondrial COX3 gene in S. cerevisiae.
The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.
Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing.
Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2.
Cloning and characterization of COX18, a Saccharomyces cerevisiae PET gene required for the assembly of cytochrome oxidase.
Copper ion-sensing transcription factor Mac1p post-translationally controls the degradation of its target gene product Ctr1p.
MAC1, a nuclear regulatory protein related to Cu-dependent transcription factors is involved in Cu/Fe utilization and stress resistance in yeast.
Phosphorylation and Cu+ coordination-dependent DNA binding of the transcription factor Mac1p in the regulation of copper transport.
A gene family homologous to the S-phase specific gene in higher plants is essential for cell proliferation in Saccharomyces cerevisiae.
Analysis of genes encoding highly conserved lysine-rich proteins in Aplysia californica and Saccharomyces cerevisiae.
An acidic sequence of a putative yeast Golgi membrane protein binds COPII and facilitates ER export.
COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast.
COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p.
COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.
COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes.
Clathrin and two components of the COPII complex, Sec23p and Sec24p, could be involved in endocytosis of the Saccharomyces cerevisiae maltose transporter.
Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex.
Dynamics of the COPII coat with GTP and stable analogues.
Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae.
Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles.
Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs.
Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting.
Shr3p mediates specific COPII coatomer-cargo interactions required for the packaging of amino acid permeases into ER-derived transport vesicles.
Specific interaction of the yeast cis-Golgi syntaxin Sed5p and the coat protein complex II component Sec24p of endoplasmic reticulum-derived transport vesicles.
Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat.
Structure of the Sec23p/24p and Sec13p/31p complexes of COPII.
Surface structure of the COPII-coated vesicle.
The early secretory pathway contributes to autophagy in yeast.
The use of liposomes to study COPII- and COPI-coated vesicle formation and membrane protein sorting.
Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake.
Competitive promoter occupancy by two yeast paralogous transcription factors controlling the multidrug resistance phenomenon.
Lpx1p is a peroxisomal lipase required for normal peroxisome morphology.
Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae.
Transcriptome profiling to identify genes involved in peroxisome assembly and function.
Cloning and expression of a yeast gene encoding a protein with ATPase activity and high identity to the subunit 4 of the human 26 S protease.
N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome.
Crystal structure of yeast mitochondrial peripheral membrane protein Tim44p C-terminal domain.
MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria.
Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor.
Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product.
Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein.
Residues of Tim44 involved in both association with the translocon of the inner mitochondrial membrane and regulation of mitochondrial Hsp70 tethering.
Class II aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA(Asp).
Identification of structurally and functionally important histidine residues in cytoplasmic aspartyl-tRNA synthetase from Saccharomyces cerevisiae.
Nucleotide sequence of the gene coding for yeast cytoplasmic aspartyl-tRNA synthetase (APS); mapping of the 5' and 3' termini of AspRS mRNA.
Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.
Sequence polymorphisms in the yeast gene encoding aspartyl tRNA synthase.
The active site of yeast aspartyl-tRNA synthetase: structural and functional aspects of the aminoacylation reaction.
The complete amino acid sequence of cytoplasmic aspartyl-tRNA synthetase from Saccharomyces cerevisiae.
Aconitase couples metabolic regulation to mitochondrial DNA maintenance.
Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity.
Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate.
Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase.
The aconitase C-terminal domain is an independent dual targeting element.
The mitochondrial targeting sequence tilts the balance between mitochondrial and cytosolic dual localization.
Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.
Yeast aconitase in two locations and two metabolic pathways: seeing small amounts is believing.
A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle.
Saccharomyces cerevisiae cell lysis mutations cly5 and cly7 define temperature-sensitive alleles of PKC1, the gene encoding yeast protein kinase C.
Organization of the yeast URA2 gene: identification of a defective dihydroorotase-like domain in the multifunctional carbamoylphosphate synthetase-aspartate transcarbamylase complex.
A novel yeast screen for mitotic arrest mutants identifies DOC1, a new gene involved in cyclin proteolysis.
Doc1 mediates the activity of the anaphase-promoting complex by contributing to substrate recognition.
Implications for the ubiquitination reaction of the anaphase-promoting complex from the crystal structure of the Doc1/Apc10 subunit.
The Doc1 subunit is a processivity factor for the anaphase-promoting complex.
Enkephalins are transported by a novel eukaryotic peptide uptake system.
Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.
Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation.
Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA.
DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray.
Slx9p facilitates efficient ITS1 processing of pre-rRNA in Saccharomyces cerevisiae.
Global protein expression profiling of budding yeast in response to DNA damage.
Yeast mitochondrial F1F0-ATPase exists as a dimer: identification of three dimer-specific subunits.
A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-ATPase.
An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis.
Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae.
Apg2p functions in autophagosome formation on the perivacuolar structure.
Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs association of complex I to the pre-autophagosomal structure in Saccharomyces cerevisiae.
Characterization of an ERAD gene as VPS30/ATG6 reveals two alternative and functionally distinct protein quality control pathways: one for soluble Z variant of human alpha-1 proteinase inhibitor (A1PiZ) and another for aggregates of A1PiZ.
Early stages of the secretory pathway, but not endosomes, are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae.
Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway.
Mutant fibrinogen cleared from the endoplasmic reticulum via endoplasmic reticulum-associated protein degradation and autophagy: an explanation for liver disease.
Peroxisome degradation in Saccharomyces cerevisiae is dependent on machinery of macroautophagy and the Cvt pathway.
Regulation of APG14 expression by the GATA-type transcription factor Gln3p.
Self-interaction is critical for Atg9 transport and function at the phagophore assembly site during autophagy.
Structure and function of Vps15 in the endosomal G protein signaling pathway.
The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae.
The Cdc48 protein and its cofactor Vms1 are involved in Cdc13 protein degradation.
Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae.
Characterization of COX9, the nuclear gene encoding the yeast mitochondrial protein cytochrome c oxidase subunit VIIa. Subunit VIIa lacks a leader peptide and is an essential component of the holoenzyme.
Chromosomal localization and expression of CBS1, a translational activator of cytochrome b in yeast.
Mitochondrial import of cytochrome c oxidase subunit VIIa in Saccharomyces cerevisiae. Identification of sequences required for mitochondrial localization in vivo.
Purification and some properties of an IMP-specific 5'-nucleotidase from yeast.
The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase.
Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo.
Protein phosphatase methyltransferase 1 (Ppm1p) is the sole activity responsible for modification of the major forms of protein phosphatase 2A in yeast.
Structure of protein phosphatase methyltransferase 1 (PPM1), a leucine carboxyl methyltransferase involved in the regulation of protein phosphatase 2A activity.
Regulators of pseudohyphal differentiation in Saccharomyces cerevisiae identified through multicopy suppressor analysis in ammonium permease mutant strains.
The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit.
Purification and properties of ferrochelatase from the yeast Saccharomyces cerevisiae. Evidence for a precursor form of the protein.
The ferrochelatase from Saccharomyces cerevisiae. Sequence, disruption, and expression of its structural gene HEM15.
The nucleotide sequence of the ferrochelatase and tRNA(val) gene region from Saccharomyces cerevisiae.
Comprehensive analysis of a yeast lipase family in the Yarrowia clade.
Cic1, an adaptor protein specifically linking the 26S proteasome to its substrate, the SCF component Cdc4.
The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.
Functional isolation of the Candida albicans FCR3 gene encoding a bZip transcription factor homologous to Saccharomyces cerevisiae Yap3p.
Isolation of a putative Candida albicans transcriptional regulator involved in pleiotropic drug resistance by functional complementation of a pdr1 pdr3 mutation in Saccharomyces cerevisiae.
Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p.
Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.
Assembly of the mitochondrial membrane system. Characterization of a yeast nuclear gene involved in the processing of the cytochrome b pre-mRNA.
The Cbp2 protein stimulates the splicing of the omega intron of yeast mitochondria.
Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1.
Aha1 binds to the middle domain of Hsp90, contributes to client protein activation, and stimulates the ATPase activity of the molecular chaperone.
Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation.
Novel predicted RNA-binding domains associated with the translation machinery.
CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae.
Cse1p functions as the nuclear export receptor for importin alpha in yeast.
Cse1p is involved in export of yeast importin alpha from the nucleus.
Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae.
Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p.
Structural basis for the assembly of a nuclear export complex.
Dbp9p, a member of the DEAD box protein family, exhibits DNA helicase activity.
Dbp9p, a putative ATP-dependent RNA helicase involved in 60S-ribosomal-subunit biogenesis, functionally interacts with Dbp6p.
Three cell wall mannoproteins facilitate the uptake of iron in Saccharomyces cerevisiae.
A novel mechanism regulates H(2) S and SO(2) production in Saccharomyces cerevisiae.
A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.
Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.
Molecular genetics of the VDAC ion channel: structural model and sequence analysis.
Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report.
The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane.
Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene.
X-ray structure of yeast Hal2p, a major target of lithium and sodium toxicity, and identification of framework interactions determining cation sensitivity.
ABF1 Ser-720 is a predominant phosphorylation site for casein kinase II of Saccharomyces cerevisiae.
ABF1-binding sites promote efficient global genome nucleotide excision repair.
Asymmetric positioning of nucleosomes and directional establishment of transcriptionally silent chromatin by Saccharomyces cerevisiae silencers.
Blurring of high-resolution data shows that the effect of intrinsic nucleosome occupancy on transcription factor binding is mostly regional, not local.
Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae.
Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene.
Extensive role of the general regulatory factors, Abf1 and Rap1, in determining genome-wide chromatin structure in budding yeast.
Functional and physical interactions between autonomously replicating sequence-binding factor 1 and the nuclear transport machinery.
General regulatory factors (GRFs) as genome partitioners.
Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae.
Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast.
Identification of a multifunctional domain in autonomously replicating sequence-binding factor 1 required for transcriptional activation, DNA replication, and gene silencing.
In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiae.
RNA polymerase II and III transcription factors can stimulate DNA replication by modifying origin chromatin structures.
Rapid chip-based capillary electrophoretic mobility shift assay with negative pressure injection for the binding study of transcription factor Abf1 in Saccharomyces cerevisiae.
Saccharomyces cerevisiae Cdc6 stimulates Abf1 DNA binding activity.
Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases.
Sequence, expression and mutational analysis of BAF1, a transcriptional activator and ARS1-binding protein of the yeast Saccharomyces cerevisiae.
Similarity between the transcriptional silencer binding proteins ABF1 and RAP1.
The gene encoding ARS-binding factor I is essential for the viability of yeast.
The multifunctional protein OBF1 is phosphorylated at serine and threonine residues in Saccharomyces cerevisiae.
The yeast Rad7/Rad16/Abf1 complex generates superhelical torsion in DNA that is required for nucleotide excision repair.
Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin.
Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair.
Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast.
Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae.
Ash1p is a site-specific DNA-binding protein that actively represses transcription.
Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells.
Identification of asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene.
Localization of ASH1 mRNA particles in living yeast.
Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA.
Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene.
Transport of axl2p depends on erv14p, an ER-vesicle protein related to the Drosophila cornichon gene product.
Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck.
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.
Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.
Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target.
Crystal structure of the 14-subunit RNA polymerase I.
Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I.
Functional architecture of RNA polymerase I.
RNA polymerase I structure and transcription regulation.
Rpa12p, a conserved RNA polymerase I subunit with two functional domains.
Sequence of a 4.8 kb fragment of Saccharomyces cerevisiae chromosome II including three essential open reading frames.
Structural basis of transcription: RNA polymerase II at 2.8 A resolution.
Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution.
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.
Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center.
Subunits shared by eukaryotic nuclear RNA polymerases.
The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.
A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.
A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties.
Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X.
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
A dominant mitochondrial mutator phenotype of Saccharomyces cerevisiae conferred by msh1 alleles altered in the sequence encoding the ATP-binding domain.
Analysis of the functional domains of the mismatch repair homologue Msh1p and its role in mitochondrial genome maintenance.
Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.
Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins.
Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae.
Purification and characterization of MSH1, a yeast mitochondrial protein that binds to DNA mismatches.
Repair of oxidative damage in mitochondrial DNA of Saccharomyces cerevisiae: involvement of the MSH1-dependent pathway.
The effect of DNA mismatches on the ATPase activity of MSH1, a protein in yeast mitochondria that recognizes DNA mismatches.
The role of 3'-5' exonucleolytic proofreading and mismatch repair in yeast mitochondrial DNA error avoidance.
Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux.
Bud10p directs axial cell polarization in budding yeast and resembles a transmembrane receptor.
Cell cycle programs of gene expression control morphogenetic protein localization.
Cloning and sequence of REV7, a gene whose function is required for DNA damage-induced mutagenesis in Saccharomyces cerevisiae.
Mutational and hyperexpression-induced disruption of bipolar budding in yeast.
O-glycosylation of Axl2/Bud10p by Pmt4p is required for its stability, localization, and function in daughter cells.
Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein.
The roles of bud-site-selection proteins during haploid invasive growth in yeast.
Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1.
Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae.
Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex.
Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.
The mechanism of tail-anchored protein insertion into the ER membrane.
WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane.
Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae.
Formation of cristae and crista junctions in mitochondria depends on antagonism between Fcj1 and Su e/g.
A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase.
Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae.
Functional domains of the rsp5 ubiquitin-protein ligase.
Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies.
NPI1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase.
NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae.
PY motifs of Rod1 are required for binding to Rsp5 and for drug resistance.
Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5.
Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2.
Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae.
Rsp5-Bul1/2 complex is necessary for the HSE-mediated gene expression in budding yeast.
Structure and function of a HECT domain ubiquitin-binding site.
The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions.
Transduction of the nitrogen signal activating Gln3-mediated transcription is independent of Npr1 kinase and Rsp5-Bul1/2 ubiquitin ligase in Saccharomyces cerevisiae.
Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast.
Yeast Rsp5 ubiquitin ligase affects the actin cytoskeleton in vivo and in vitro.
Proteasomes are essential for yeast proliferation. cDNA cloning and gene disruption of two major subunits.
Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae.
Phosphorylation is required for high-affinity binding of DBP, a yeast mitochondrial site-specific RNA binding protein.
Purification and characterization of an RNA dodecamer sequence binding protein from mitochondria of Saccharomyces cerevisiae.
Rmd9p controls the processing/stability of mitochondrial mRNAs and its overexpression compensates for a partial deficiency of oxa1p in Saccharomyces cerevisiae.
The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12.3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII.
The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3'-UTRs of yeast mitochondrial mRNAs.
Translation initiation in Saccharomyces cerevisiae mitochondria: functional interactions among mitochondrial ribosomal protein Rsm28p, initiation factor 2, methionyl-tRNA-formyltransferase and novel protein Rmd9p.
MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p.
Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions.
Molecular cloning and characterization of the Saccharomyces cerevisiae CYT2 gene encoding cytochrome-c1-heme lyase.
A ubiquitin-like system mediates protein lipidation.
ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.
Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae.
Atg4 proteolytic activity can be inhibited by Atg1 phosphorylation.
Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis.
Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion.
Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole.
Conserved Atg8 recognition sites mediate Atg4 association with autophagosomal membranes and Atg8 deconjugation.
Dual roles of Atg8-PE deconjugation by Atg4 in autophagy.
Induction of autophagic flux by amino acid deprivation is distinct from nitrogen starvation-induced macroautophagy.
Isolation of autophagocytosis mutants of Saccharomyces cerevisiae.
Starvation-induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease.
The mitochondrial phosphatidylserine decarboxylase Psd1 is involved in nitrogen starvation-induced mitophagy in yeast.
The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway.
The yeast autophagy protease Atg4 is regulated by thioredoxin.
Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy.
Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria.
The deca-GX3 proteins Yae1-Lto1 function as adaptors recruiting the ABC protein Rli1 for iron-sulfur cluster insertion.
The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly.
The function of ORAOV1/LTO1, a gene that is overexpressed frequently in cancer: essential roles in the function and biogenesis of the ribosome.
Seripauperins of Saccharomyces cerevisiae: a new multigene family encoding serine-poor relatives of serine-rich proteins.
Differentiating mechanisms of toxicity using global gene expression analysis in Saccharomyces cerevisiae.
Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p.
The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex.
An engineered Saccharomyces cerevisiae strain binds the broadly neutralizing human immunodeficiency virus type 1 antibody 2G12 and elicits mannose-specific gp120-binding antibodies.
Identification and characterization of a novel yeast gene: the YGP1 gene product is a highly glycosylated secreted protein that is synthesized in response to nutrient limitation.
Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall.
Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence.
A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3.
CEP3 encodes a centromere protein of Saccharomyces cerevisiae.
Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function.
Genetic and epigenetic regulation of the FLO gene family generates cell-surface variation in yeast.
Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae.
A yeast strain biosensor to detect cell wall-perturbing agents.
Analysis of yeast protein kinases using protein chips.
Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria.
The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases.
A novel NADH kinase is the mitochondrial source of NADPH in Saccharomyces cerevisiae.
Characterization of NADH kinase from Saccharomyces cerevisiae.
POS5 gene of Saccharomyces cerevisiae encodes a mitochondrial NADH kinase required for stability of mitochondrial DNA.
Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control.
COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression.
COMPASS: a complex of proteins associated with a trithorax-related SET domain protein.
Histone H1 in Saccharomyces cerevisiae: a double mystery solved?
Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae.
Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria.
The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae.
Gpi1, a Saccharomyces cerevisiae protein that participates in the first step in glycosylphosphatidylinositol anchor synthesis.
Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER.
A late Golgi sorting function for Saccharomyces cerevisiae Apm1p, but not for Apm2p, a second yeast clathrin AP medium chain-related protein.
Adaptor complex-independent clathrin function in yeast.
The alternate AP-1 adaptor subunit Apm2 interacts with the Mil1 regulatory protein and confers differential cargo sorting.
Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation.
Mnd1 is required for meiotic interhomolog repair.
Mnd1p: an evolutionarily conserved protein required for meiotic recombination.
The Mnd1 protein forms a complex with Hop2 to promote homologous chromosome pairing and meiotic double-strand break repair.
An intramolecular interaction within the lipid kinase Fab1 regulates cellular phosphatidylinositol 3,5-bisphosphate lipid levels.
Assembly of a Fab1 phosphoinositide kinase signaling complex requires the Fig4 phosphoinositide phosphatase.
Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis.
Isolation of yeast mutants defective for localization of vacuolar vital dyes.
Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast.
Osmotic stress-induced increase of phosphatidylinositol 3,5-bisphosphate requires Vac14p, an activator of the lipid kinase Fab1p.
Phosphatidylinositol 3,5-bisphosphate regulates the transition between trans-SNARE complex formation and vacuole membrane fusion.
Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member.
The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover.
The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae.
VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.
Gpi19, the Saccharomyces cerevisiae homologue of mammalian PIG-P, is a subunit of the initial enzyme for glycosylphosphatidylinositol anchor biosynthesis.
A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress.
A proposal for nomenclature of aldehyde dehydrogenases in Saccharomyces cerevisiae and characterization of the stress-inducible ALD2 and ALD3 genes.
Response to acetaldehyde stress in the yeast Saccharomyces cerevisiae involves a strain-dependent regulation of several ALD genes and is mediated by the general stress response pathway.
Specialization of function among aldehyde dehydrogenases: the ALD2 and ALD3 genes are required for beta-alanine biosynthesis in Saccharomyces cerevisiae.
Importin 7 and importin alpha/importin beta are nuclear import receptors for the glucocorticoid receptor.
Importin/karyopherin protein family members required for mRNA export from the nucleus.
Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase.
Nuclear import of Ho endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system.
Nuclear import of zinc binuclear cluster proteins proceeds through multiple, overlapping transport pathways.
Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export.
Network of general and specialty J protein chaperones of the yeast cytosol.
The J-protein family: modulating protein assembly, disassembly and translocation.
Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells.
A phosphatase complex that dephosphorylates gamma-H2AX regulates DNA damage checkpoint recovery.
A role for Saccharomyces cerevisiae histone H2A in DNA repair.
Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites.
DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain.
Distribution and dynamics of chromatin modification induced by a defined DNA double-strand break.
Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification.
Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications.
The two yeast histone H2A genes encode similar protein subtypes.
Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system.
Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.
Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome.
Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae.
Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease.
Functions of the DNA damage response pathway target Ho endonuclease of yeast for degradation via the ubiquitin-26S proteasome system.
Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly.
Cloning and characterization of a Saccharomyces cerevisiae gene encoding the low molecular weight protein-tyrosine phosphatase.
Crystal structures of a low-molecular weight protein tyrosine phosphatase from Saccharomyces cerevisiae and its complex with the substrate p-nitrophenyl phosphate.
Structural and mechanistic basis for the activation of a low-molecular weight protein tyrosine phosphatase by adenine.
A myosin light chain mediates the localization of the budding yeast IQGAP-like protein during contractile ring formation.
Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V.
Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae.
Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.
Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p.
Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications.
Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis.
MER1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis.
A novel endoplasmic reticulum membrane protein Rcr1 regulates chitin deposition in the cell wall of Saccharomyces cerevisiae.
Cloning and characterization of GPD2, a second gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1.
Distinct intracellular localization of Gpd1p and Gpd2p, the two yeast isoforms of NAD+-dependent glycerol-3-phosphate dehydrogenase, explains their different contributions to redox-driven glycerol production.
Physiological response to anaerobicity of glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae.
The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation.
BCS1, a novel gene required for the expression of functional Rieske iron-sulfur protein in Saccharomyces cerevisiae.
Internal targeting signal of the BCS1 protein: a novel mechanism of import into mitochondria.
Cold-shock induction of a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae.
Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences.
The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.
Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria.
Functional cooperation of mitochondrial protein import receptors in yeast.
Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex.
Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.
Dual cell wall/mitochondria localization of the 'SUN' family proteins.
S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative state.
SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces cerevisiae and produced dependently on oxygen level.
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
MAP kinase dynamics in response to pheromones in budding yeast.
MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation.
Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.
Specificity of MAP kinase signaling in yeast differentiation involves transient versus sustained MAPK activation.
The DAC2/FUS3 protein kinase is not essential for transcriptional activation of the mating pheromone response pathway in Saccharomyces cerevisiae.
The Saccharomyces cerevisiae Sub2 protein suppresses heterochromatic silencing at telomeres and subtelomeric genes.
Asymmetric structure of the yeast F1 ATPase in the absence of bound nucleotides.
Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants.
Mutations in the mitochondrial ATP synthase gamma subunit suppress a slow-growth phenotype of yme1 yeast lacking mitochondrial DNA.
Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase.
Studies on the ATP3 gene of Saccharomyces cerevisiae: presence of two closely linked copies, ATP3a and ATP3b, on the right arm of chromosome II.
Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor.
Drug-induced phenotypes provide a tool for the functional analysis of yeast genes.
Screening of genes involved in isooctane tolerance in Saccharomyces cerevisiae by using mRNA differential display.
YLR099C (ICT1) encodes a soluble Acyl-CoA-dependent lysophosphatidic acid acyltransferase responsible for enhanced phospholipid synthesis on organic solvent stress in Saccharomyces cerevisiae.
Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b.
A Saccharomyces cerevisiae homolog of the human adrenoleukodystrophy transporter is a heterodimer of two half ATP-binding cassette transporters.
The ABC transporter proteins Pat1 and Pat2 are required for import of long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae.
The human peroxisomal ABC half transporter ALDP functions as a homodimer and accepts acyl-CoA esters.
The yeast YKL741 gene situated on the left arm of chromosome XI codes for a homologue of the human ALD protein.
Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.
The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast.
The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles.
Topological restriction of SNARE-dependent membrane fusion.
Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis.
Endoplasmic reticulum membrane localization of Rce1p and Ste24p, yeast proteases involved in carboxyl-terminal CAAX protein processing and amino-terminal a-factor cleavage.
The CaaX proteases, Afc1p and Rce1p, have overlapping but distinct substrate specificities.
Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as conserved interactors of BLOC-1.
Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces cerevisiae.
Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions.
Maturation and intranuclear transport of pre-ribosomes requires Noc proteins.
Nucleotide sequence of RAD4 gene of Saccharomyces cerevisiae that can be propagated in Escherichia coli without inactivation.
Crystal structure of yeast Ypr118w, a methylthioribose-1-phosphate isomerase related to regulatory eIF2B subunits.
LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum.
Multiple motif scanning to identify methyltransferases from the yeast proteome.
Probabilistic approach to predicting substrate specificity of methyltransferases.
DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein.
MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy.
Regulation of the localization of Dbf2 and Mob1 during cell division of Saccharomyces cerevisiae.
Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit.
Structural and functional analysis of Saccharomyces cerevisiae Mob1.
The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization.
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit.
Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.
The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation.
A novel Rad24 checkpoint protein complex closely related to replication factor C.
Characterization of the five replication factor C genes of Saccharomyces cerevisiae.
Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway.
Elg1 forms an alternative RFC complex important for DNA replication and genome integrity.
Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae.
Mechanical link between cohesion establishment and DNA replication: Ctf7p/Eco1p, a cohesion establishment factor, associates with three different replication factor C complexes.
Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex.
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex.
The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae.
Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint.
Molecular genetics of cryptopleurine resistance in Saccharomyces cerevisiae: expression of a ribosomal protein gene family.
The yeast Ty3 retrotransposon contains a 5'-3' bipartite primer-binding site and encodes nucleocapsid protein NCp9 functionally homologous to HIV-1 NCp7.
CLD1 reverses the ubiquinone insufficiency of mutant cat5/coq7 in a Saccharomyces cerevisiae model system.
Deacylation on the matrix side of the mitochondrial inner membrane regulates cardiolipin remodeling.
Deletion of the cardiolipin-specific phospholipase Cld1 rescues growth and life span defects in the tafazzin mutant: implications for Barth syndrome.
Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the effects of cardiolipin peroxidation.
Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae.
Identification of a cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeast.
Lipidomics characterization of biosynthetic and remodeling pathways of cardiolipins in genetically and nutritionally manipulated yeast cells.
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export.
Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue.
Amino-terminal extension generated from an upstream AUG codon is not required for mitochondrial import of yeast N2,N2-dimethylguanosine-specific tRNA methyltransferase.
Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity.
Genome-wide screen for inner nuclear membrane protein targeting in Saccharomyces cerevisiae: roles for N-acetylation and an integral membrane protein.
Location of N2,N2-dimethylguanosine-specific tRNA methyltransferase.
Mechanism and a peptide motif for targeting peripheral proteins to the yeast inner nuclear membrane.
Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase.
Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast.
Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene.
Proteome survey reveals modularity of the yeast cell machinery.
The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p.
Nuclear genes encoding the yeast mitochondrial ATPase complex. Analysis of ATP1 coding the F1-ATPase alpha-subunit and its assembly.
Structure of Utp21 tandem WD domain provides insight into the organization of the UTPB complex involved in ribosome synthesis.
Identification and characterization of Elf1, a conserved transcription elongation factor in Saccharomyces cerevisiae.
ACR1, a gene encoding a protein related to mitochondrial carriers, is essential for acetyl-CoA synthetase activity in Saccharomyces cerevisiae.
Identification of the yeast ACR1 gene product as a succinate-fumarate transporter essential for growth on ethanol or acetate.
Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae.
The crystal structure of yeast thiamin pyrophosphokinase.
Functional characterization of nuclear localization signals in yeast Sm proteins.
The yeast SME1 gene encodes the homologue of the human E core protein.
Cryo-EM and MD infer water-mediated proton transport and autoinhibition mechanisms of Vo complex.
Structural comparison of the vacuolar and Golgi V-ATPases from Saccharomyces cerevisiae.
Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain.
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.
Iwr1 directs RNA polymerase II nuclear import.
RNA-directed DNA methylation and plant development require an IWR1-type transcription factor.
The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.
A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity.
A new yeast translation initiation factor suppresses a mutation in the eIF-4A RNA helicase.
A ribosome-specialized translation initiation pathway is required for cap-dependent translation of vesicular stomatitis virus mRNAs.
Chl4p and Iml3p are two new members of the budding yeast outer kinetochore.
Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene.
Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for mitochondrial function and morphology.
Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex.
The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology.
A scalable method for integration and functional analysis of multiple microarray datasets.
GUT2, a gene for mitochondrial glycerol 3-phosphate dehydrogenase of Saccharomyces cerevisiae.
Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast.
The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae.
An Lsm2-Lsm7 complex in Saccharomyces cerevisiae associates with the small nucleolar RNA snR5.
Characterization of Sm-like proteins in yeast and their association with U6 snRNA.
Crystal structures of the Lsm complex bound to the 3' end sequence of U6 small nuclear RNA.
Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.
Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes.
The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs.
Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae.
Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF.
High functional overlap between MluI cell-cycle box binding factor and Swi4/6 cell-cycle box binding factor in the G1/S transcriptional program in Saccharomyces cerevisiae.
Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways.
Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues.
In vivo disassembly of free polyubiquitin chains by yeast Ubp14 modulates rates of protein degradation by the proteasome.
A dynamin-like protein encoded by the yeast sporulation gene SPO15.
A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting.
DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames.
Vps1p, a member of the dynamin GTPase family, is necessary for Golgi membrane protein retention in Saccharomyces cerevisiae.
An efficient method to isolate yeast genes causing overexpression-mediated growth arrest.
The inner membrane protein Mdm33 controls mitochondrial morphology in yeast.
Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system.
Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation.
RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases.
Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain.
Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase.
Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion.
The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail: regulation of secretion from the endoplasmic reticulum.
A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore.
Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.
NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.
Slk19p is a centromere protein that functions to stabilize mitotic spindles.
A conserved transcriptional regulator governs fungal morphology in widely diverged species.
Complete sequence of the Saccharomyces cerevisiae LEU1 gene encoding isopropylmalate isomerase.
Yeast LEU1. Repression of mRNA levels by leucine and relationship of 5'-noncoding region to that of LEU2.
Role of lysine 240 in the mechanism of yeast pyruvate kinase catalysis.
The allosteric regulation of pyruvate kinase by fructose-1,6-bisphosphate.
The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae.
The yeast pyruvate kinase gene does not contain a string of non-preferred codons: revised nucleotide sequence.
D-tyrosyl-tRNA(Tyr) metabolism in Saccharomyces cerevisiae.
Metabolism of D-aminoacyl-tRNAs in Escherichia coli and Saccharomyces cerevisiae cells.
Activation of the checkpoint kinase Rad53 by the phosphatidyl inositol kinase-like kinase Mec1.
An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae.
Characterization of mec1 kinase-deficient mutants and of new hypomorphic mec1 alleles impairing subsets of the DNA damage response pathway.
Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins.
Esc4p, a new target of Mec1p (ATR), promotes resumption of DNA synthesis after DNA damage.
LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae.
MEC3, MEC1, and DDC2 are essential components of a telomere checkpoint pathway required for cell cycle arrest during senescence in Saccharomyces cerevisiae.
Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair.
Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase.
Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways.
Role of the C-terminus of Mec1 checkpoint kinase in its localization to sites of DNA damage.
The ATM homologue MEC1 is required for phosphorylation of replication protein A in yeast.
The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast.
Yeast histone 2A serine 129 is essential for the efficient repair of checkpoint-blind DNA damage.
Biochemical characterization, membrane association and identification of amino acids essential for the function of Alg11 from Saccharomyces cerevisiae, an alpha1,2-mannosyltransferase catalysing two sequential glycosylation steps in the formation of the lipid-linked core oligosaccharide.
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum.
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating.
Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.
The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane.
Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
A multiprotein complex that interacts with RNA polymerase II elongator.
An early step in wobble uridine tRNA modification requires the Elongator complex.
Architecture of the yeast Elongator complex.
Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae.
Crystal structure of elongator subcomplex Elp4-6.
Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo.
Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.
Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation.
Molecular architecture of the yeast Elongator complex reveals an unexpected asymmetric subunit arrangement.
Molecular architecture, structure-function relationship, and importance of the Elp3 subunit for the RNA binding of holo-elongator.
Molecular basis of tRNA recognition by the Elongator complex.
Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.
Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein.
RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes.
Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin.
Structural and functional analysis of the killer element pPin1-3 from Pichia inositovora.
Structural insights into the function of Elongator.
The Elongator subcomplex Elp456 is a hexameric RecA-like ATPase.
A Saccharomyces cerevisiae cyclophilin resident in the endoplasmic reticulum.
Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles.
STT10, a novel class-D VPS yeast gene required for osmotic integrity related to the PKC1/STT1 protein kinase pathway.
The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport.
Yeast Vps45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles.
Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.
Precursor binding to yeast mitochondria. A general role for the outer membrane protein Mas70p.
Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70.
Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast.
The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins.
Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli.
Light-driven enzymatic catalysis of DNA repair: a review of recent biophysical studies on photolyase.
Purification of the yeast PHR1 photolyase from an Escherichia coli overproducing strain and characterization of the intrinsic chromophores of the enzyme.
Sequence of the Saccharomyces cerevisiae PHR1 gene and homology of the PHR1 photolyase to E. coli photolyase.
The role of conserved amino acids in substrate binding and discrimination by photolyase.
Crystal structure of the oxygen-dependant coproporphyrinogen oxidase (Hem13p) of Saccharomyces cerevisiae.
Isolation, sequence, and regulation by oxygen of the yeast HEM13 gene coding for coproporphyrinogen oxidase.
Purification and properties of coproporphyrinogen oxidase from the yeast Saccharomyces cerevisiae.
Identification of protein coding regions by database similarity search.
Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase.
Nucleotide sequence and characterization of the Saccharomyces cerevisiae RPL19A gene encoding a homolog of the mammalian ribosomal protein L19.
Organization and characterization of the two yeast ribosomal protein YL19 genes.
Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe.
Amino acid sequence similarity between GCN3 and GCD2, positive and negative translational regulators of GCN4: evidence for antagonism by competition.
SRC1: an intron-containing yeast gene involved in sister chromatid segregation.
Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing.
Ctf19p: a novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle.
The N-terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain.
Swc2 is a widely conserved H2AZ-binding module essential for ATP-dependent histone exchange.
A RING-H2 finger motif is essential for the function of Der3/Hrd1 in endoplasmic reticulum associated protein degradation in the yeast Saccharomyces cerevisiae.
Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.
Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins.
Endoplasmic reticulum degradation requires lumen to cytosol signaling. Transmembrane control of Hrd1p by Hrd3p.
Genetic interactions of Hrd3p and Der3p/Hrd1p with Sec61p suggest a retro-translocation complex mediating protein transport for ER degradation.
HRD gene dependence of endoplasmic reticulum-associated degradation.
Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation.
In vivo action of the HRD ubiquitin ligase complex: mechanisms of endoplasmic reticulum quality control and sterol regulation.
Membrane topology and function of Der3/Hrd1p as a ubiquitin-protein ligase (E3) involved in endoplasmic reticulum degradation.
Retrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1p.
Role of 26S proteasome and HRD genes in the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein.
The Hrd1p ligase complex forms a linchpin between ER-lumenal substrate selection and Cdc48p recruitment.
Crystal structure of the yeast ribosomal protein rpS3 in complex with its chaperone Yar1.
Molecular architecture of a eukaryotic translational initiation complex.
The ribosomal protein gene RPS3 is an essential single copy gene of the yeast Saccharomyces cerevisiae.
New potential cell wall glucanases of Saccharomyces cerevisiae and their involvement in mating.
Characterization of the putative maltose transporters encoded by YDL247w and YJR160c.
Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.
Exportin 1 (Crm1p) is an essential nuclear export factor.
Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1.
A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase.
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis.
Over-expression of S. cerevisiae G1 cyclins restores the viability of alg1 N-glycosylation mutants.
The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae.
The X-ray structure of the DNA-binding domain from the Saccharomyces cerevisiae cell-cycle transcription factor Mbp1 at 2.1-A resolution.
The Rad4 homologue YDR314C is essential for strand-specific repair of RNA polymerase I-transcribed rDNA in Saccharomyces cerevisiae.
Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress.
Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions.
Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems.
Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA.
Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast.
Chemogenomic profiling: identifying the functional interactions of small molecules in yeast.
A yeast GTPase-activating protein that interacts specifically with a member of the Ypt/Rab family.
Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids.
Following the flux of long-chain bases through the sphingolipid pathway in vivo using mass spectrometry.
Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p.
Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro.
Mass spectrometry-based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae.
Requirement of sphingolipid alpha-hydroxylation for fungicidal action of syringomycin E.
Sterol-dependent regulation of sphingolipid metabolism in Saccharomyces cerevisiae.
Synthesis of monohydroxylated inositolphosphorylceramide (IPC-C) in Saccharomyces cerevisiae requires Scs7p, a protein with both a cytochrome b5-like domain and a hydroxylase/desaturase domain.
The crystal structure of an integral membrane fatty acid alpha-hydroxylase.
Crystal structure of eucaryotic E3, lipoamide dehydrogenase from yeast.
Genetics of the synthesis of serine from glycine and the utilization of glycine as sole nitrogen source by Saccharomyces cerevisiae.
Nucleotide sequence for yeast dihydrolipoamide dehydrogenase.
The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae: comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites.
The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascomycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine.
Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1.
Identification of a vacuole-associated metalloreductase and its role in Ctr2-mediated intracellular copper mobilization.
Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae.
Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes.
The metalloreductase Fre6p in Fe-efflux from the yeast vacuole.
The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function.
Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p.
Molecular cloning and analysis of the yeast flocculation gene FLO1.
Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae.
Region of FLO1 proteins responsible for sugar recognition.
Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae.
The Saccharomyces cerevisiae FLO1 flocculation gene encodes for a cell surface protein.
The retention mechanism of cell wall proteins in Saccharomyces cerevisiae. Wall-bound Cwp2p is beta-1,6-glucosylated.
3-isopropylmalate is the major endogenous substrate of the Saccharomyces cerevisiae trans-aconitate methyltransferase.
Identification of the gene and characterization of the activity of the trans-aconitate methyltransferase from Saccharomyces cerevisiae.
Secreted 3-isopropylmalate methyl ester signals invasive growth during amino acid starvation in Saccharomyces cerevisiae.
An In Vitro TORC1 Kinase Assay That Recapitulates the Gtr-Independent Glutamine-Responsive TORC1 Activation Mechanism on Yeast Vacuoles.
Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane.
LAS24/KOG1, a component of the TOR complex 1 (TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast.
Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.
Identification and functional characterization of yeast zeta-COP.
Early expression of yeast genes affected by chemical stress.
Vhr1p, a new transcription factor from budding yeast, regulates biotin-dependent expression of VHT1 and BIO5.
Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast.
Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast.
Metalloregulation of yeast membrane steroid receptor homologs.
DOT4 links silencing and cell growth in Saccharomyces cerevisiae.
Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae.
Maintenance of low histone ubiquitylation by Ubp10 correlates with telomere-proximal Sir2 association and gene silencing.
Reversal of PCNA ubiquitylation by Ubp10 in Saccharomyces cerevisiae.
Splitting the task: Ubp8 and Ubp10 deubiquitinate different cellular pools of H2BK123.
The deubiquitinating enzyme Dot4p is involved in regulating nutrient uptake.
The histone deubiquitinating enzyme Ubp10 is involved in rDNA locus control in Saccharomyces cerevisiae by affecting Sir2p association.
Transcriptional profiling of ubp10 null mutant reveals altered subtelomeric gene expression and insurgence of oxidative stress response.
Ubp10/Dot4p regulates the persistence of ubiquitinated histone H2B: distinct roles in telomeric silencing and general chromatin.
Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.
New regulators of a high affinity Ca2+ influx system revealed through a genome-wide screen in yeast.
The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae.
The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation.
Pho91 is a vacuolar phosphate transporter that regulates phosphate and polyphosphate metabolism in Saccharomyces cerevisiae.
Phosphate transport and sensing in Saccharomyces cerevisiae.
The Rsp5 E3 ligase mediates turnover of low affinity phosphate transporters in Saccharomyces cerevisiae.
The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase.
Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae.
Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor.
Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III.
cis- and trans-Acting determinants of transcription termination by yeast RNA polymerase II.
An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2.
Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy.
Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.
Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein.
Importin-beta-like nuclear transport receptors.
Kap104p imports the PY-NLS-containing transcription factor Tfg2p into the nucleus.
Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and RNA.
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame.
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
An interaction between frataxin and Isu1/Nfs1 that is crucial for Fe/S cluster synthesis on Isu1.
Binding of yeast frataxin to the scaffold for Fe-S cluster biogenesis, Isu.
Frataxin interacts functionally with mitochondrial electron transport chain proteins.
Frataxin interacts with Isu1 through a conserved tryptophan in its beta-sheet.
Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase.
Mitochondrial iron detoxification is a primary function of frataxin that limits oxidative damage and preserves cell longevity.
Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly.
Structural basis of the iron storage function of frataxin from single-particle reconstruction of the iron-loaded oligomer.
The structures of frataxin oligomers reveal the mechanism for the delivery and detoxification of iron.
The yeast frataxin homologue mediates mitochondrial iron efflux. Evidence for a mitochondrial iron cycle.
Yeast and human frataxin are processed to mature form in two sequential steps by the mitochondrial processing peptidase.
A member of a novel family of yeast 'Zn-finger' proteins mediates the transition from stationary phase to cell proliferation.
Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function.
The Gcs1 and Age2 ArfGAP proteins provide overlapping essential function for transport from the yeast trans-Golgi network.
The yeast ADP-ribosylation factor GAP, Gcs1p, is involved in maintenance of mitochondrial morphology.
Different import pathways through the mitochondrial intermembrane space for inner membrane proteins.
The role of the TIM8-13 complex in the import of Tim23 into mitochondria.
The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins.
The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism.
The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
Analysis of an 11.7 kb DNA fragment of chromosome XI reveals a new tRNA gene and four new open reading frames including a leucine zipper protein and a homologue to the yeast mitochondrial regulator ABF2.
Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.
Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.
High-resolution DNA-binding specificity analysis of yeast transcription factors.
Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase.
Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit.
Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein.
SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae.
SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma.
The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductase.
The covalent attachment of FAD to the flavoprotein of Saccharomyces cerevisiae succinate dehydrogenase is not necessary for import and assembly into mitochondria.
The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studies.
A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae.
The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria.
The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease.
Coordinate regulation of sugar flux and translation by PAS kinase.
PAS kinase: an evolutionarily conserved PAS domain-regulated serine/threonine kinase.
Regulation of glucose partitioning by PAS kinase and Ugp1 phosphorylation.
Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae.
Characterization of SFP2, a putative sulfate permease gene of Saccharomyces cerevisiae.
Isolation of a cDNA from Saccharomyces cerevisiae that encodes a high affinity sulphate transporter at the plasma membrane.
Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae.
Cloning and sequencing of the gene encoding the soluble fumarate reductase from Saccharomyces cerevisiae.
Investigation by 13C-NMR and tricarboxylic acid (TCA) deletion mutant analysis of pathways for succinate formation in Saccharomyces cerevisiae during anaerobic fermentation.
One of the fumarate reductase isoenzymes from Saccharomyces cerevisiae is encoded by the OSM1 gene.
Role in anaerobiosis of the isoenzymes for Saccharomyces cerevisiae fumarate reductase encoded by OSM1 and FRDS1.
Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth.
The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast.
The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae.
The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae.
Isolation of the human PEX12 gene, mutated in group 3 of the peroxisome biogenesis disorders.
Pex12p of Saccharomyces cerevisiae is a component of a multi-protein complex essential for peroxisomal matrix protein import.
Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import.
The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enzyme-selective manner.
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.
Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast.
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1.
The RHC21 gene of budding yeast, a homologue of the fission yeast rad21+ gene, is essential for chromosome segregation.
Characterization and mutagenesis of the gene encoding the A49 subunit of RNA polymerase A in Saccharomyces cerevisiae.
Localization of the yeast RNA polymerase I-specific subunits.
RNA polymerase I contains a TFIIF-related DNA-binding subcomplex.
A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 protein selectively cleaves the beta-phosphate from 5-diphosphoinositol pentakisphosphate (5pp-ip5).
A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae.
Molecular architecture of the inositol phosphatase Siw14.
Phylogenetic and genetic linkage between novel atypical dual-specificity phosphatases from non-metazoan organisms.
Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates.
Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase.
Ornithine decarboxylase from Saccharomyces cerevisiae. Purification, properties, and regulation of activity.
Polyamines regulate their synthesis by inducing expression and blocking degradation of ODC antizyme.
The biochemistry, genetics, and regulation of polyamine biosynthesis in Saccharomyces cerevisiae.
The gene and the primary structure of ornithine decarboxylase from Saccharomyces cerevisiae.
Identification and classification of genes required for tolerance to freeze-thaw stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strains.
Identification of genes required for maximal tolerance to high-glucose concentrations, as those present in industrial alcoholic fermentation media, through a chemogenomics approach.
Cloning and characterization of KNR4, a yeast gene involved in (1,3)-beta-glucan synthesis.
Interaction of Knr4 protein, a protein involved in cell wall synthesis, with tyrosine tRNA synthetase encoded by TYS1 in Saccharomyces cerevisiae.
KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, is involved in the transcriptional control of chitin synthase genes.
Yeast calmodulin and a conserved nuclear protein participate in the in vivo binding of a matrix association region.
Molecular characterization of a copper transport protein in S. cerevisiae: an unexpected role for copper in iron transport.
The Saccharomyces cerevisiae copper transport protein (Ctr1p). Biochemical characterization, regulation by copper, and physiologic role in copper uptake.
A third essential DNA polymerase in S. cerevisiae.
DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae.
Eukaryotic DNA polymerases require an iron-sulfur cluster for the formation of active complexes.
Roles for DNA polymerase delta in initiating and terminating leading strand DNA replication.
Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae.
Structure, biosynthesis, and localization of dipeptidyl aminopeptidase B, an integral membrane glycoprotein of the yeast vacuole.
Dot1p modulates silencing in yeast by methylation of the nucleosome core.
Role for the silencing protein Dot1 in meiotic checkpoint control.
Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9.
Structure of the conserved core of the yeast Dot1p, a nucleosomal histone H3 lysine 79 methyltransferase.
Ubiquitination of histone H2B by Rad6 is required for efficient Dot1-mediated methylation of histone H3 lysine 79.
Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis.
Crystal structures and catalytic mechanism of the C-methyltransferase Coq5 provide insights into a key step of the yeast coenzyme Q synthesis pathway.
The COQ5 gene encodes a yeast mitochondrial protein necessary for ubiquinone biosynthesis and the assembly of the respiratory chain.
Yeast Coq5 C-methyltransferase is required for stability of other polypeptides involved in coenzyme Q biosynthesis.
A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein.
Characterization of Ccw7p cell wall proteins and the encoding genes of Saccharomyces cerevisiae wine yeast strains: relevance for flor formation.
Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae.
Increased mortality of Saccharomyces cerevisiae cell wall protein mutants.
Minisatellites in Saccharomyces cerevisiae genes encoding cell wall proteins: a new way towards wine strain characterisation.
Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall.
Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae.
Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein.
Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock.
Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae.
NOP3 is an essential yeast protein which is required for pre-rRNA processing.
SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.
Saccharomyces cerevisiae SSB1 protein and its relationship to nucleolar RNA-binding proteins.
Sbp1p affects translational repression and decapping in Saccharomyces cerevisiae.
A functional GTPase domain, but not its transmembrane domain, is required for function of the SRP receptor beta-subunit.
Signal recognition particle receptor is important for cell growth and protein secretion in Saccharomyces cerevisiae.
Rbl2p, a yeast protein that binds to beta-tubulin and participates in microtubule function in vivo.
Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.
Consequences of growth media, gene copy number, and regulatory mutations on the expression of the PRB1 gene of Saccharomyces cerevisiae.
Genetic properties of mutations at the PEP4 locus in Saccharomyces cerevisiae.
Heritable activity: a prion that propagates by covalent autoactivation.
Processing pathway for protease B of Saccharomyces cerevisiae.
Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.
Protein degradation, meiosis and sporulation in proteinase-deficient mutants of Saccharomyces cerevisiae.
A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage.
Bdf1, a yeast chromosomal protein required for sporulation.
Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2.
Bromodomain factor 1 (Bdf1) protein interacts with histones.
Bromodomain factor 1 corresponds to a missing piece of yeast TFIID.
Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries.
Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation.
Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA.
Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1.
The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast.
The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs.
Functional requirement of a site-specific ribose methylation in ribosomal RNA.
MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase.
A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing.
PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p.
Removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scERV1 gene product to mitochondria.
Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA.
TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway.
A yeast gene that is essential for release from glucose repression encodes a protein kinase.
ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase.
An inhibited conformation for the protein kinase domain of the Saccharomyces cerevisiae AMPK homolog Snf1.
Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1.
Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1.
Glucose repression of yeast mitochondrial transcription: kinetics of derepression and role of nuclear genes.
Glucose-responsive regulators of gene expression in Saccharomyces cerevisiae function at the nuclear periphery via a reverse recruitment mechanism.
High-affinity glucose transport in Saccharomyces cerevisiae is under general glucose repression control.
Key role of Ser562/661 in Snf1-dependent regulation of Cat8p in Saccharomyces cerevisiae and Kluyveromyces lactis.
Mammalian AMP-activated protein kinase shares structural and functional homology with the catalytic domain of yeast Snf1 protein kinase.
Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase.
Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein.
N-terminal mutations modulate yeast SNF1 protein kinase function.
PAS kinase is activated by direct SNF1-dependent phosphorylation and mediates inhibition of TORC1 through the phosphorylation and activation of Pbp1.
Protein kinase Snf1 is involved in the proper regulation of the unfolded protein response in Saccharomyces cerevisiae.
Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.
Snf1 phosphorylates adenylate cyclase and negatively regulates protein kinase A-dependent transcription in Saccharomyces cerevisiae.
Snf1-dependent transcription confers glucose-induced decay upon the mRNA product.
Structural insight into the autoinhibition mechanism of AMP-activated protein kinase.
Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family.
Structure and expression of the SNF1 gene of Saccharomyces cerevisiae.
Subunits of the Snf1 kinase heterotrimer show interdependence for association and activity.
Sumoylation regulates the SNF1 protein kinase.
The CCR1 (SNF1) and SCH9 protein kinases act independently of cAMP-dependent protein kinase and the transcriptional activator ADR1 in controlling yeast ADH2 expression.
The SNF1 kinase ubiquitin-associated domain restrains its activation, activity, and the yeast life span.
The Saccharomyces cerevisiae AMPK, Snf1, negatively regulates the Hog1 MAPK pathway in ER stress response.
Yeast Pak1 kinase associates with and activates Snf1.
Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response.
Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion.
Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery.
Ctf7p/Eco1p exhibits acetyltransferase activity -- but does it matter?
The spindle pole body assembly component Mps3p/Nep98p functions in sister chromatid cohesion.
20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals.
Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes.
Structure of the HOM2 gene of Saccharomyces cerevisiae and regulation of its expression.
A second gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae.
Regulation of yeast H(+)-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters.
The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae.
ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p.
Expression of an ATP-binding cassette transporter-encoding gene (YOR1) is required for oligomycin resistance in Saccharomyces cerevisiae.
Functional characterization of the Saccharomyces cerevisiae ABC-transporter Yor1p overexpressed in plasma membranes.
Identification of interdependent signals required for anterograde traffic of the ATP-binding cassette transporter protein Yor1p.
Inhibiting endoplasmic reticulum (ER)-associated degradation of misfolded Yor1p does not permit ER export despite the presence of a diacidic sorting signal.
Mutational disruption of plasma membrane trafficking of Saccharomyces cerevisiae Yor1p, a homologue of mammalian multidrug resistance protein.
Role of the yeast ABC transporter Yor1p in cadmium detoxification.
The multidrug resistance-associated protein (MRP) subfamily (Yrs1/Yor1) of Saccharomyces cerevisiae is important for the tolerance to a broad range of organic anions.
A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase.
Histidine methylation of yeast ribosomal protein Rpl3p is required for proper 60S subunit assembly.
Methylation of yeast ribosomal protein Rpl3 promotes translational elongation fidelity.
Nucleotide sequence of the tcm1 gene (ribosomal protein L3) of Saccharomyces cerevisiae.
Separate membrane targeting and anchoring domains function in the localization of the S. cerevisiae Cdc24p guanine nucleotide exchange factor.
Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption.
Identification of the upstream activation sequences responsible for the expression and regulation of the PEM1 and PEM2 genes encoding the enzymes of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae.
Mutations in the Saccharomyces cerevisiae opi3 gene: effects on phospholipid methylation, growth and cross-pathway regulation of inositol synthesis.
Phosphatidylethanolamine methyltransferase and phospholipid methyltransferase activities from Saccharomyces cerevisiae. Enzymological and kinetic properties.
Quantitative profiling of PE, MMPE, DMPE, and PC lipid species by multiple precursor ion scanning: a tool for monitoring PE metabolism.
Regulation of phosphatidylethanolamine methyltransferase and phospholipid methyltransferase by phospholipid precursors in Saccharomyces cerevisiae.
Regulation of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae.
Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants.
The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression.
Yeast mutant defective in phosphatidylcholine synthesis.
Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes.
Cloning and characterization of the mitochondrial phosphate transport protein gene from the yeast Saccharomyces cerevisiae.
Isolation and characterization of the gene for a yeast mitochondrial import receptor.
Amino acid transporter genes are essential for FLO11-dependent and FLO11-independent biofilm formation and invasive growth in Saccharomyces cerevisiae.
Amino acids regulate the intracellular trafficking of the general amino acid permease of Saccharomycescerevisiae.
Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease.
Concentrative sorting of secretory cargo proteins into COPII-coated vesicles.
Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases.
Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast.
GAP1, a novel selection and counter-selection marker for multiple gene disruptions in Saccharomyces cerevisiae.
GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression.
GGA proteins bind ubiquitin to facilitate sorting at the trans-Golgi network.
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease.
NH4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains.
Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae.
Permease recycling and ubiquitination status reveal a particular role for Bro1 in the multivesicular body pathway.
The fenpropimorph resistance gene FEN2 from Saccharomyces cerevisiae encodes a plasma membrane H+-pantothenate symporter.
Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae.
Transport activity-dependent intracellular sorting of the yeast general amino acid permease.
Two transcription factors, Gln3p and Nil1p, use the same GATAAG sites to activate the expression of GAP1 of Saccharomyces cerevisiae.
Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1.
Uptake of putrescine and spermidine by Gap1p on the plasma membrane in Saccharomyces cerevisiae.
Sequence of the yeast gene RVS 161 located on chromosome III.
The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes.
Yeast mutant affected for viability upon nutrient starvation: characterization and cloning of the RVS161 gene.
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin.
The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1.
The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain.
The ribosome-bound chaperones RAC and Ssb1/2p are required for accurate translation in Saccharomyces cerevisiae.
Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners.
A SNARE required for retrograde transport to the endoplasmic reticulum.
Yeast functional analysis: identification of two essential genes involved in ER to Golgi trafficking.
MYST protein acetyltransferase activity requires active site lysine autoacetylation.
Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex.
The role of Sas2, an acetyltransferase homologue of Saccharomyces cerevisiae, in silencing and ORC function.
The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae.
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.
Yeast SAS silencing genes and human genes associated with AML and HIV-1 Tat interactions are homologous with acetyltransferases.
Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex.
Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis.
Evolution of the clock from yeast to man by period-doubling folds in the cellular oscillator.
Transcriptional regulation of nonfermentable carbon utilization in budding yeast.
SNG1 -- a new gene involved in nitrosoguanidine resistance in Saccharomyces cerevisiae.