The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.
The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation.
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.
Aquifex aeolicus tRNA (Gm18) methyltransferase has unique substrate specificity. TRNA recognition mechanism of the enzyme.
Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus.
Crystal structure of LpxC, a zinc-dependent deacetylase essential for endotoxin biosynthesis.
Site-directed mutagenesis of the bacterial metalloamidase UDP-(3-O-acyl)-N-acetylglucosamine deacetylase (LpxC). Identification of the zinc binding site.
UDP-3-O-((R)-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase functions through a general acid-base catalyst pair mechanism.
A structure-based model of the reaction catalyzed by lumazine synthase from Aquifex aeolicus.
Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii.
X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 A resolution: determinants of thermostability revealed from structural comparisons.
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis.
Nucleotide and partner-protein control of bacterial replicative helicase structure and function.
Crystal structure of the full-length bacterial selenocysteine-specific elongation factor SelB.
Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.
Aquifex aeolicus dihydroorotase: association with aspartate transcarbamoylase switches on catalytic activity.
Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.
The crystal structure of a novel, latent dihydroorotase from Aquifex aeolicus at 1.7A resolution.
The mononuclear metal center of type-I dihydroorotase from Aquifex aeolicus.
Acta Crystallogr Sect F Struct Biol Cryst Commun
Acta Crystallogr. F Struct. Biol. Commun.
Crystal structures, dynamics and functional implications of molybdenum-cofactor biosynthesis protein MogA from two thermophilic organisms.
Change in structure and ligand binding properties of hyperstable cytochrome c555 from Aquifex aeolicus by domain swapping.
Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an alpha-Helix-Inserted Circular Permutant of Cytochrome c555.
Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an alpha-Helix-Inserted Circular Permutant of Cytochrome cpisub>555pi/sub>.
Hyperstability and crystal structure of cytochrome c(555) from hyperthermophilic Aquifex aeolicus.
Structure of the torque ring of the flagellar motor and the molecular basis for rotational switching.
Ligation reaction specificities of an NAD(+)-dependent DNA ligase from the hyperthermophile Aquifex aeolicus.
Structural mechanism of GAF-regulated sigma(54) activators from Aquifex aeolicus.
Structure, function, and tethering of DNA-binding domains in sigma transcriptional activators.
Structure, function, and tethering of DNA-binding domains in sigma⁵⁴ transcriptional activators.
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea.
A 3.3A-Resolution Structure of Hyperthermophilic Respiratory ComplexIII Reveals the Mechanism of Its Thermal Stability.
A 3.3 A-Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of Its Thermal Stability.
Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.
Structure of a complex of the ATPase SecA and the protein-translocation channel.
Aquifex aeolicus tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA.
Crystal structure of trbp111: a structure-specific tRNA-binding protein.
A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA.
Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage.
Structure of Aquifex aeolicus argonaute highlights conformational flexibility of the PAZ domain as a potential regulator of RNA-induced silencing complex function.
A naturally chimeric type IIA topoisomerase in Aquifex aeolicus highlights an evolutionary path for the emergence of functional paralogs.
Crystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus.
Structures and reaction mechanisms of the two related enzymes, PurN and PurU.
Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus.
Bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase, the key enzyme for di-myo-inositol-phosphate synthesis in several (hyper)thermophiles.
Structure of small protein B: the protein component of the tmRNA-SmpB system for ribosome rescue.
Visualizing tmRNA entry into a stalled ribosome.
Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus at 2.6 A resolution: a novel methyltransferase fold.
A lipid gating mechanism for the channel-forming O antigen ABC transporter.
Architecture of a channel-forming O-antigen polysaccharide ABC transporter.
Cryo-EM structure of the full-length WzmWzt ABC transporter required for lipid-linked O antigen transport.
A competitive inhibitor traps LeuT in an open-to-out conformation.
A conserved leucine occupies the empty substrate site of LeuT in the Na(+)-free return state.
Antidepressant binding site in a bacterial homologue of neurotransmitter transporters.
Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures.
Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation.
Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters.
Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.
Insights into transport mechanism from LeuT engineered to transport tryptophan.
LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake.
Psychomotor impairments and therapeutic implications revealed by a mutation associated with infantile Parkinsonism-Dystonia.
Psychomotor impairments and therapeutic implications revealed by a mutation associated with infantile Parkinsonism-Dystonia. .
Structural basis for action by diverse antidepressants on biogenic amine transporters.
Structural origins of nitroxide side chain dynamics on membrane protein alpha-helical sites.
Structural, functional, and behavioral insights of dopamine dysfunction revealed by a deletion in SLC6A3.
Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context.
Substrate binds in the S1 site of the F253A mutant of LeuT, a neurotransmitter sodium symporter homologue.
X-ray structure of LeuT in an inward-facing occluded conformation reveals mechanism of substrate release.
X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.
Breaking sieve for steric exclusion of a noncognate amino acid from active site of a tRNA synthetase.
The C-Ala domain brings together editing and aminoacylation functions on one tRNA.
Structure of a Type-1 Secretion System ABC Transporter.
Crystal structure of the 'PhoU-like' phosphate uptake regulator from Aquifex aeolicus.
Sulfide:quinone oxidoreductase in membranes of the hyperthermophilic bacterium Aquifex aeolicus (VF5).
The structure of Aquifex aeolicus sulfide:quinone oxidoreductase, a basis to understand sulfide detoxification and respiration.
Structures of a histone deacetylase homologue bound to the TSA and SAHA inhibitors.
NMR structure of the hypothetical protein AQ-1857 encoded by the Y157 gene from Aquifex aeolicus reveals a novel protein fold.
Crystal structure of the tRNA processing enzyme RNase PH from Aquifex aeolicus.
Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase.
MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition.
Negative regulation of AAA + ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria.
Nucleotide-induced asymmetry within ATPase activator ring drives sigma54-RNAP interaction and ATP hydrolysis.
Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains.
DNA recognition by a sigma(54) transcriptional activator from Aquifex aeolicus.
Structure and regulatory mechanism of Aquifex aeolicus NtrC4: variability and evolution in bacterial transcriptional regulation.
Biosynthesis of isoprenoids: crystal structure of the [4Fe-4S] cluster protein IspG.
LytB protein catalyzes the terminal step of the 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis.
Structure of (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, the terminal enzyme of the non-mevalonate pathway.
Collaboration between CC- and A-adding enzymes to build and repair the 3'-terminal CCA of tRNA in Aquifex aeolicus.
Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase.
Crystal structure of THEP1 from the hyperthermophile Aquifex aeolicus: a variation of the RecA fold.
Thermophile-specific proteins: the gene product of aq_1292 from Aquifex aeolicus is an NTPase.
Crystal structure of Aquifex aeolicus sigmaN bound to promoter DNA and the structure of sigmaN-holoenzyme.
Crystal structure of pii>Aquifex aeolicuspi/i> sigmapisup>Npi/sup> bound to promoter DNA and the structure of sigmapisup>Npi/sup>-holoenzyme.
Structural basis of DNA recognition by the alternative sigma-factor, sigma54.
Structure of the RNA polymerase core-binding domain of sigma(54) reveals a likely conformational fracture point.
The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70.
Protein design by fusion: implications for protein structure prediction and evolution.
Similar modes of polypeptide recognition by export chaperones in flagellar biosynthesis and type III secretion.
Crystal structure of the flagellar sigma/anti-sigma complex sigma(28)/FlgM reveals an intact sigma factor in an inactive conformation.
Crystal structure of type I 3-dehydroquinate dehydratase of Aquifex aeolicus suggests closing of active site flap is not essential for enzyme action.
Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.
The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.
Structure of the periplasmic adaptor protein from a major facilitator superfamily (MFS) multidrug efflux pump.
The Lipid A 1-Phosphatase, LpxE, Functionally Connects Multiple Layers of Bacterial Envelope Biogenesis.
Insights on a new PDI-like family: structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus.
Function of His185 in Aquifex aeolicus 3-deoxy-D-manno-octulosonate 8-phosphate synthase.
Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus.
A unique beta-1,2-mannosyltransferase of Thermotoga maritima that uses di-myo-inositol phosphate as the mannosyl acceptor.
Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetases.
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.
Structure of D-lactate dehydrogenase from Aquifex aeolicus complexed with NAD(+) and lactic acid (or pyruvate).
A spring-loaded state of NusG in its functional cycle is suggested by X-ray crystallography and supported by site-directed mutants.
Crystal structures of transcription factor NusG in light of its nucleic acid- and protein-binding activities.
Measurement of acceptor-TC helix length of tRNA for terminal A76-addition by A-adding enzyme.
Structural basis for template-independent RNA polymerization.
Structure of the whole cytosolic region of ATP-dependent protease FtsH.
Crystal structure of MraY, an essential membrane enzyme for bacterial cell wall synthesis.
GlcNAc-1-P-transferase-tunicamycin complex structure reveals basis for inhibition of N-glycosylation.
Structural insights into inhibition of lipid I production in bacterial cell wall synthesis.
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.
Characterization and structure of the Aquifex aeolicus protein DUF752: a bacterial tRNA-methyltransferase (MnmC2) functioning without the usually fused oxidase domain (MnmC1).
Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Crosstalk between primase subunits can act to regulate primer synthesis in trans.
The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA.
Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus.
Minimal and RNA-free RNase P in Aquifex aeolicus.
A hyperthermophilic plant-type [2Fe-2S] ferredoxin from Aquifex aeolicus is stabilized by a disulfide bond.
Probing FtsZ and tubulin with C8-substituted GTP analogs reveals differences in their nucleotide binding sites.
Structural insights into the conformational variability of FtsZ.
Intrinsic motions along an enzymatic reaction trajectory.
Structure of the biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus at 2.2 A resolution.
Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis.
WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis.
Free and ATP-bound structures of Ap4A hydrolase from Aquifex aeolicus V5.
Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase.
MinCD cell division proteins form alternating copolymeric cytomotive filaments.
Structural and biochemical analyses of shikimate dehydrogenase AroE from Aquifex aeolicus: implications for the catalytic mechanism.
Structural studies of thiamin monophosphate kinase in complex with substrates and products.
Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus.
Crystal structure of chorismate synthase from Aquifex aeolicus reveals a novel beta alpha beta sandwich topology.