Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences.
Crystal structure of Cmr2 suggests a nucleotide cyclase-related enzyme in type III CRISPR-Cas systems.
Crystal structure of the Cmr2-Cmr3 subcomplex in the CRISPR-Cas RNA silencing effector complex.
RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex.
Structural model of a CRISPR RNA-silencing complex reveals the RNA-target cleavage activity in Cmr4.
Structure of an RNA silencing complex of the CRISPR-Cas immune system.
Structure of the Cmr2 subunit of the CRISPR-Cas RNA silencing complex.
Structure of the Cmr2-Cmr3 subcomplex of the Cmr RNA silencing complex.
Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution.
Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus.
Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus.
Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus.
Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA.
The DNA polymerase from the archaebacterium Pyrococcus furiosus does not testify for a specific relationship between archaebacteria and eukaryotes.
The DNA polymerase gene from the hyperthermophilic marine archaebacterium, Pyrococcus furiosus, shows sequence homology with alpha-like DNA polymerases.
Dps-like protein from the hyperthermophilic archaeon Pyrococcus furiosus.
Active-site models for complexes of quinolinate synthase with substrates and intermediates.
Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus.
Characterization of the locus encoding the [Ni-Fe] sulfhydrogenase from the archaeon Pyrococcus furiosus: evidence for a relationship to bacterial sulfite reductases.
Enzymes of hydrogen metabolism in Pyrococcus furiosus.
Hydrogenases I and II from Pyrococcus furiosus.
On the prosthetic groups of the NiFe sulfhydrogenase from Pyrococcus furiosus: topology, structure, and temperature-dependent redox chemistry.
Redox properties of the sulfhydrogenase from Pyrococcus furiosus.
Domains of Pyrococcus furiosus L-asparaginase fold sequentially and assemble through strong intersubunit associative forces.
Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action.
N-terminal domain of Pyrococcus furiosus L-asparaginase functions as a non-specific, stable, molecular chaperone.
Structural and functional insights into an archaeal L-asparaginase obtained through the linker-less assembly of constituent domains.
Structural stability and functional analysis of L-asparaginase from Pyrococcus furiosus.
Structure determination of a new protein from backbone-centered NMR data and NMR-assisted structure prediction.
Structural basis for adenylate kinase activity in ABC ATPases.
Structural biochemistry of ATP-driven dimerization and DNA-stimulated activation of SMC ATPases.
Structure and DNA-binding activity of the Pyrococcus furiosus SMC protein hinge domain.
The evolution of SMC proteins: phylogenetic analysis and structural implications.
Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima.
Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate.
5S rRNA binding proteins from the hyperthermophilic archaeon, Pyrococcus furiosus.
An asymmetric SMC-kleisin bridge in prokaryotic condensin.
Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization.
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Crystallization and preliminary X-ray crystallographic analysis of putative tRNA-modification enzymes from Pyrococcus furiosus and Thermus thermophilus.
The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N2-methylguanosine at position 6 in tRNA.
The N-terminal domain of TFIIB from Pyrococcus furiosus forms a zinc ribbon.
(NZ)CH...O contacts assist crystallization of a ParB-like nuclease.
The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism.
Solution structure of the chitin-binding domain 1 (ChBD1) of a hyperthermophilic chitinase from Pyrococcus furiosus.
An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA.
Anaerobic microbes: oxygen detoxification without superoxide dismutase.
Evidence of recent lateral gene transfer among hyperthermophilic archaea.
Genomic sequence of hyperthermophile, Pyrococcus furiosus: implications for physiology and enzymology.
Inward-facing conformation of a multidrug resistance MATE family transporter.
Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.
Molecular and biochemical characterization of a distinct type of fructose-1,6-bisphosphatase from Pyrococcus furiosus.
Archaeal transcription: function of an alternative transcription factor B from Pyrococcus furiosus.
The RPB7 orthologue E' is required for transcriptional activity of a reconstituted archaeal core enzyme at low temperatures and stimulates open complex formation.
Transcription factor E is a part of transcription elongation complexes.
Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA.
Formation of the conserved pseudouridine at position 55 in archaeal tRNA.
NMR data collection and analysis protocol for high-throughput protein structure determination.
Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein.
Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus.
Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction.
Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus.
Archaeal fructose-1,6-bisphosphate aldolases constitute a new family of archaeal type class I aldolases.
Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.
Silver ion incorporation and nanoparticle formation inside the cavity of Pyrococcus furiosus ferritin: structural and size-distribution analyses.
Large-scale computational discovery and analysis of virus-derived microbial nanocompartments.
Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus.
Structural insight into ubiquitin-like protein recognition and oligomeric states of JAMM/MPN+ proteases.
The beta-propeller domain of the trilobed protease from Pyrococcus furiosus reveals an open Velcro topology.
MutS2 family protein from Pyrococcus furiosus.
The crystal structure of a novel glucosamine-6-phosphate deaminase from the hyperthermophilic archaeon Pyrococcus furiosus.
Acta Crystallogr Sect F Struct Biol Cryst Commun
Acta Crystallogr. F Struct. Biol. Commun.
Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
Isolation of the gene encoding Pyrococcus furiosus ornithine carbamoyltransferase and study of its expression profile in vivo and in vitro.
Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation.
Crystal structure of archaeal homolog of proteasome-assembly chaperone PbaA.
Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.
Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme.
DJ-1 family Maillard deglycases prevent acrylamide formation.
Purification and characterization of two functional forms of intracellular protease PfpI from the hyperthermophilic archaeon Pyrococcus furiosus.
Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.
Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools.
The crystal structure of Pyrococcus furiosus RecJ implicates it as an ancestor of eukaryotic Cdc45.
The closed structure of an archaeal DNA ligase from Pyrococcus furiosus.
The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation.
Archaeal primase: bridging the gap between RNA and DNA polymerases.
Crystal structure of a DNA-dependent RNA polymerase (DNA primase).
The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus.
Fast native-SAD phasing for routine macromolecular structure determination.
Biochemical adaptations of two sugar kinases from the hyperthermophilic archaeon Pyrococcus furiosus.
Cloning, purification, crystallization and preliminary crystallographic analysis of galactokinase from Pyrococcus furiosus.
Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase.
GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus.
Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus.
Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase.
Metabolic channeling of carbamoyl phosphate, a thermolabile intermediate: evidence for physical interaction between carbamate kinase-like carbamoyl-phosphate synthetase and ornithine carbamoyltransferase from the hyperthermophile Pyrococcus furiosus.
Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants.
Refined structure of Pyrococcus furiosus ornithine carbamoyltransferase at 1.87 A.
The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures.
Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P.
Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors.
Functional reconstitution and characterization of Pyrococcus furiosus RNase P.
Structure of Pfu Pop5, an archaeal RNase P protein.
Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein.
Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (Prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus.
Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase by analysis of targeted mutants.
Crystallization and characterization of the prolidase from Pyrococcus furiosus.
Proline dipeptidase from Pyrococcus furiosus.
Structure of the prolidase from Pyrococcus furiosus.
A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization.
The P. furiosus mre11/rad50 complex promotes 5' strand resection at a DNA double-strand break.
Acetyl coenzyme A synthetase (ADP forming) from the hyperthermophilic Archaeon pyrococcus furiosus: identification, cloning, separate expression of the encoding genes, acdAI and acdBI, in Escherichia coli, and in vitro reconstitution of the active heterotetrameric enzyme from its recombinant subunits.
Purification and characterization of two reversible and ADP-dependent acetyl coenzyme A synthetases from the hyperthermophilic archaeon Pyrococcus furiosus.
Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus.
Characterization of a novel zinc-containing, lysine-specific aminopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.
Pyrrolidone carboxyl peptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis.
Thermal stability of pyrrolidone carboxyl peptidases from the hyperthermophilic Archaeon, Pyrococcus furiosus.
X-ray crystalline structures of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus, and its Cys-free mutant.
Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI.
Ribonucleotide reductase in the archaeon Pyrococcus furiosus: a critical enzyme in the evolution of DNA genomes?
Citrate synthase from the hyperthermophilic Archaeon, Pyrococcus furiosus.
The crystal structure of citrate synthase from the hyperthermophilic archaeon Pyrococcus furiosus at 1.9-A resolution.
Characterization of the TrmB-like protein, PF0124, a TGM-recognizing global transcriptional regulator of the hyperthermophilic archaeon Pyrococcus furiosus.
Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase.
Characterization and gene deletion analysis of four homologues of group 3 pyridine nucleotide disulfide oxidoreductases from Thermococcus kodakarensis.
Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus.
Discovery and characterization of a coenzyme A disulfide reductase from Pyrococcus horikoshii. Implications for this disulfide metabolism of anaerobic hyperthermophiles.
Insights into the metabolism of elemental sulfur by the hyperthermophilic archaeon Pyrococcus furiosus: characterization of a coenzyme A-dependent NAD(P)H sulfur oxidoreductase.
Crystal structure of a novel carboxypeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.
Purification and characterization of a cobalt-activated carboxypeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.
Novel type of glucose-6-phosphate isomerase in the hyperthermophilic archaeon Pyrococcus furiosus.
The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily.
A topA intein in Pyrococcus furiosus and its relatedness to the r-gyr intein of Methanococcus jannaschii.
Overexpression, physicochemical characterization, and modeling of a hyperthermophilic pyrococcus furiosus type 2 IPP isomerase.
Structural Mechanism for the Temperature-Dependent Activation of the Hyperthermophilic Pf2001 Esterase.
Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus.
Unraveling the structural and functional differences between purine nucleoside phosphorylase and 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus.
Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm.
Identification of a novel helicase activity unwinding branched DNAs from the hyperthermophilic archaeon, Pyrococcus furiosus.
The archaeal Hjm helicase has recQ-like functions, and may be involved in repair of stalled replication fork.
Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.
Crystal structure of Cmr5 from Pyrococcus furiosus and its functional implications.
Methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus. Mechanism of the reaction and assignment of disulfide bonds.
Purification and characterization of 5'-methylthioadenosine phosphorylase from the hyperthermophilic archaeon Pyrococcus furiosus: substrate specificity and primary structure analysis.
Crystal structure of a novel non-Pfam protein PF2046 solved using low resolution B-factor sharpening and multi-crystal averaging methods.
Structural fold, conservation and Fe(II) binding of the intracellular domain of prokaryote FeoB.
Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus.
Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.
Purification and properties of acetyl-CoA synthetase (ADP-forming), an archaeal enzyme of acetate formation and ATP synthesis, from the hyperthermophile Pyrococcus furiosus.
1H NMR investigation of the electronic and molecular structure of the four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus. Identification of Asp 14 as a cluster ligand in each of the four redox states.
1H NMR investigation of the paramagnetic cluster environment in Pyrococcus furiosus three-iron ferredoxin: sequence-specific assignment of ligated cysteines independent of tertiary structure.
A pure S=3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus ferredoxin.
Cloning, expression, and molecular characterization of the gene encoding an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
Effect of serinate ligation at each of the iron sites of the [Fe4S4] cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and biological properties.
Participation of the disulfide bridge in the redox cycle of the ferredoxin from the hyperthermophile Pyrococcus furiosus: 1H nuclear magnetic resonance time resolution of the four redox states at ambient temperature.
Proton NMR investigation of the oxidized three-iron clusters in the ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and Thermococcus litoralis.
Site-directed mutations of the 4Fe-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus: role of the cluster-coordinating aspartate in physiological electron transfer reactions.
Solution 1H NMR determination of secondary structure for the three-iron form of ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
Solution NMR characterization of the thermodynamics of the disulfide bond orientational isomerism and its effect of cluster electronic properties for the hyperthermostable three-iron cluster ferredoxin from the archaeon Pyrococcus furiosus.
The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.
The 1.5-A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon Pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight into substrate binding and stability in carbamate kinases.
The carbamate kinase-like carbamoyl phosphate synthetase of the hyperthermophilic archaeon Pyrococcus furiosus, a missing link in the evolution of carbamoyl phosphate biosynthesis.
A Holliday junction resolvase from Pyrococcus furiosus: functional similarity to Escherichia coli RuvC provides evidence for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea.
Biochemical analysis of replication factor C from the hyperthermophilic archaeon Pyrococcus furiosus.
Crystal structure of an archaeal DNA sliding clamp: proliferating cell nuclear antigen from Pyrococcus furiosus.
Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea.
Intermolecular ion pairs maintain the toroidal structure of Pyrococcus furiosus PCNA.
Crystal structure of the archaeal heat shock regulator from Pyrococcus furiosus: a molecular chimera representing eukaryal and bacterial features.
Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus.
DNA repair systems in archaea: mementos from the last universal common ancestor?
Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus.
Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli.
Isolation, sequence and characterization of the maltose-regulated mlrA gene from the hyperthermophilic archaeum Pyrococcus furiosus.
Phosphoenolpyruvate synthetase from the hyperthermophilic archaeon Pyrococcus furiosus.
Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus.
Function of MoaB proteins in the biosynthesis of the molybdenum and tungsten cofactors.
An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus.
Expression cloning and characterization of a novel gene that encodes the RNA-binding protein FAU-1 from Pyrococcus furiosus.
Cdc6/Orc1 from Pyrococcus furiosus may act as the origin recognition protein and Mcm helicase recruiter.
Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus sp.
Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus.
A simple energy-conserving system: proton reduction coupled to proton translocation.
Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.
Isolation and characterization of the hyperthermostable serine protease, pyrolysin, and its gene from the hyperthermophilic archaeon Pyrococcus furiosus.
Purification, characterization, and molecular modeling of pyrolysin and other extracellular thermostable serine proteases from hyperthermophilic microorganisms.
Structures of the superoxide reductase from Pyrococcus furiosus in the oxidized and reduced states.
RNA polymerase and transcription elongation factor Spt4/5 complex structure.
Characterization of the reverse gyrase from the hyperthermophilic archaeon Pyrococcus furiosus.
A large conformational change in the putative ATP pyrophosphatase PF0828 induced by ATP binding.
Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications.
Novel structure and redox chemistry of the prosthetic groups of the iron-sulfur flavoprotein sulfide dehydrogenase from Pyrococcus furiosus; evidence for a [2Fe-2S] cluster with Asp(Cys)3 ligands.
Sulfide dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus: a new multifunctional enzyme involved in the reduction of elemental sulfur.
Aldehyde oxidoreductases from Pyrococcus furiosus.
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase, a novel tungsten-containing enzyme with a potential glycolytic role in the hyperthermophilic archaeon Pyrococcus furiosus.
The ferredoxin-dependent conversion of glyceraldehyde-3-phosphate in the hyperthermophilic archaeon Pyrococcus furiosus represents a novel site of glycolytic regulation.
X-ray structure of RLI, an essential twin cassette ABC ATPase involved in ribosome biogenesis and HIV capsid assembly.
PF0610, a novel winged helix-turn-helix variant possessing a rubredoxin-like Zn ribbon motif from the hyperthermophilic archaeon, Pyrococcus furiosus.
A novel domain arrangement in a monomeric cyclodextrin-hydrolyzing enzyme from the hyperthermophile Pyrococcus furiosus.
The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus.
Identification of the critical region in replication factor C from Pyrococcus furiosus for the stable complex formation with proliferating cell nuclear antigen and DNA.
Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle.
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
ATP hydrolysis by RAD50 protein switches MRE11 enzyme from endonuclease to exonuclease.
ATP-driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling.
Mre11 and Rad50 from Pyrococcus furiosus: cloning and biochemical characterization reveal an evolutionarily conserved multiprotein machine.
Rad50 adenylate kinase activity regulates DNA tethering by Mre11/Rad50 complexes.
Structural biochemistry and interaction architecture of the DNA double-strand break repair Mre11 nuclease and Rad50-ATPase.
Structural biology of Rad50 ATPase: ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily.
The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair.
The rad50 signature motif: essential to ATP binding and biological function.
Tungsten transport protein A (WtpA) in Pyrococcus furiosus: the first member of a new class of tungstate and molybdate transporters.
The conserved YjgF protein family deaminates enamine/imine intermediates of pyridoxal-5'-phosphate (PLP)-dependent enzyme reactions.
Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus.
Alternative conformations of the archaeal Nop56/58-fibrillarin complex imply flexibility in box C/D RNPs.
The structure of the box C/D enzyme reveals regulation of RNA methylation.
Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family.
Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.
Multiple crystal forms of N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus.
Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference.
Parameter-space screening: a powerful tool for high-throughput crystal structure determination.
Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.
Biochemical characterization, cloning, and sequencing of ADP-dependent (AMP-forming) glucokinase from two hyperthermophilic archaea, Pyrococcus furiosus and Thermococcus litoralis.
Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: implications for a sugar-induced conformational change in ADP-dependent kinase.
Purification and characterization of a novel ADP-dependent glucokinase from the hyperthermophilic archaeon Pyrococcus furiosus.
Evolutionary relationships of bacterial and archaeal glutamine synthetase genes.
Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus- the role of conserved residues in the active site.
Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus.
Tertiary structure and carbohydrate recognition by the chitin-binding domain of a hyperthermophilic chitinase from Pyrococcus furiosus.
Comparative analysis of archaeal lipid-linked oligosaccharides that serve as oligosaccharide donors for Asn glycosylation.
Molecular characterization of phosphoglycerate mutase in archaea.
Structure determination of fibrillarin from the hyperthermophilic archaeon Pyrococcus furiosus.
A Pyrococcus homolog of the leucine-responsive regulatory protein, LrpA, inhibits transcription by abrogating RNA polymerase recruitment.
An Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus is negatively autoregulated.
Crystal structure of the Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus.
Crystallization and quaternary structure analysis of an Lrp-like regulatory protein from the hyperthermophile Pyrococcus furiosus.
The eubacterial transcriptional activator Lrp is present in the archaeon Pyrococcus furiosus.
The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence.
Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein.
Contribution of surface salt bridges to protein stability.
Determinants of protein hyperthermostability: purification and amino acid sequence of rubredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus and secondary structure of the zinc adduct by NMR.
Solution-state structure by NMR of zinc-substituted rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus.
X-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus.
Transcriptional regulation in the hyperthermophilic archaeon Pyrococcus furiosus: coordinated expression of divergently oriented genes in response to beta-linked glucose polymers.
Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism.
Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis.
The novel tungsten-iron-sulfur protein of the hyperthermophilic archaebacterium, Pyrococcus furiosus, is an aldehyde ferredoxin oxidoreductase. Evidence for its participation in a unique glycolytic pathway.
Crystal structure of a Cas6 paralogous protein from Pyrococcus furiosus.
A non-alpha-like DNA polymerase from the hyperthermophilic archaeon Pyrococcus furiosus.
Atomic structure of the clamp loader small subunit from Pyrococcus furiosus.
Mutational analysis of Pyrococcus furiosus replication factor C based on the three-dimensional structure.
Three-dimensional electron microscopy of the clamp loader small subunit from Pyrococcus furiosus.
Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.
Two archaeal tRNase Z enzymes: similar but different.
A conserved MCM single-stranded DNA binding element is essential for replication initiation.
Analysis of the crystal structure of an active MCM hexamer.
MCM ring hexamerization is a prerequisite for DNA-binding.
Structure of a double hexamer of the Pyrococcus furiosus minichromosome maintenance protein N-terminal domain.
A hyperactive NAD(P)H:Rubredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus.
In vitro reconstitution of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus.
NAD(P)H:rubredoxin oxidoreductase from Pyrococcus furiosus.
Biochemical characterization of the hjc holliday junction resolvase of Pyrococcus furiosus.
Crystal structure of the archaeal holliday junction resolvase Hjc and implications for DNA recognition.
Dissection of the regional roles of the archaeal Holliday junction resolvase Hjc by structural and mutational analyses.
Mutational analysis of the Pyrococcus furiosus holliday junction resolvase hjc revealed functionally important residues for dimer formation, junction DNA binding, and cleavage activities.
The amino acid sequence of glutamate dehydrogenase from Pyrococcus furiosus, a hyperthermophilic archaebacterium.
The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures.
Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane.
Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima.
Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.
Crystal structure of Argonaute and its implications for RISC slicer activity.
Purified Argonaute2 and an siRNA form recombinant human RISC.
Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.
Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.
Structural and functional characterization of KEOPS dimerization by Pcc1 and its role in t6A biosynthesis.
Structure of archaeal translational initiation factor 2 betagamma-GDP reveals significant conformational change of the beta-subunit and switch 1 region.
Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes.
The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1.
Crystal structure of methionine aminopeptidase from hyperthermophile, Pyrococcus furiosus.
EPR and X-ray crystallographic characterization of the product-bound form of the MnII-loaded methionyl aminopeptidase from Pyrococcus furiosus.
Methionine aminopeptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: molecular cloning and overexpression in Escherichia coli of the gene, and characteristics of the enzyme.
Overexpression and divalent metal binding properties of the methionyl aminopeptidase from Pyrococcus furiosus.
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation.
Solution structure of Pyrococcus furiosus RPP21, a component of the archaeal RNase P holoenzyme, and interactions with its RPP29 protein partner.
Solution structure of an archaeal RNase P binary protein complex: formation of the 30-kDa complex between Pyrococcus furiosus RPP21 and RPP29 is accompanied by coupled protein folding and highlights critical features for protein-protein and protein-RNA interactions.
Crystal structure of the novel lesion-specific endonuclease PfuEndoQ from Pyrococcus furiosus.
Structural basis for recognition of distinct deaminated DNA lesions by endonuclease Q.