Staphylococcus aureus subsp. aureus NCTC 8325 chromosome, complete genome.
Staphylococcus aureus subsp. aureus NCTC 8325
Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus.
The Staphylococcus aureus NCTC8325 Genome
(in) Fischetti,V., Novick,R., Ferretti,J., Portnoy,D. and Rood,J. (Eds.); GRAM POSITIVE PATHOGENS; ASM Press (2006)
Submitted (18-FEB-2006) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (27-JAN-2006) Microbiology and Immunology, The University of Oklahoma Health Sciences Center, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73104, USA
REVIEWED REFSEQ: This record has been curated by NCBI staff. The\nreference sequence was derived from CP000253.\nRefSeq Category: Reference Genome\n UPR: UniProt Genome\nStaphylococcus aureus subsp. aureus NCTC 8325 is available from\nwww.narsa.net.\nCOMPLETENESS: full length.
binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.
chromosomal replication initiation protein
binds the polymerase to DNA and acts as a sliding clamp
Required for DNA replication; binds preferentially to single-stranded, linear DNA
catalyzes the degradation of histidine to urocanate and ammmonia
catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
in Escherichia coli this protein is wrapped around the base of the L1 stalk
catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis
two-component response regulator, putative
sensory box histidine kinase VicK, putative
SPOUT methyltransferase family protein; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA
23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH
1-phosphatidylinositol phosphodiesterase precursor, putative
1-phosphatidylinositol phosphodiesterase
in group A Streptococci this protein was found to cross react with anti myosin antibodies and may play a role in rheumatic fever
staphylococcal accessory regulator-like protein
2,3-diaminopropionate biosynthesis protein SbnA
2,3-diaminopropionate biosynthesis protein SbnB
3-ketoacyl-acyl carrier protein reductase, putative
transcriptional regulator, GntR family, putative
catalyzes the reversible phosphorolysis of ribonucleosides and 2'- deoxyribonucleosides to the free base and (2'-deoxy)ribose-1- phosphate
catalyzes the formation of D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-D-ribose-5-phosphate
catalyzes the transfer of phosphate between the C1 and C5 carbons of pentose
phosphonates ABC transporter, permease protein CC0363, putative
amino acid ABC transporter, ATP-binding protein, putative
amino acid ABC transporter ATP-binding protein
phosphonate ABC transporter, substrate-binding protein, putative
phosphonate ABC transporter substrate-binding protein
replication initiation protein, putative
alcohol dehydrogenase, iron-containing, putative
bifunctional acetaldehyde-CoA/alcohol dehydrogenase
capsular polysaccharide biosynthesis protein, putative
capsular polysaccharide biosynthesis protein
capsular polysaccharide synthesis enzyme Cap5B
capsular polysaccharide biosynthesis protein Cap5B
capsular polysaccharide synthesis enzyme Cap8C
capsular polysaccharide biosynthesis protein Cap8C
capsular polysaccharide biosynthesis protein Cap5D, putative
capsular polysaccharide biosynthesis protein Cap5D
capsular polysaccharide biosynthesis protein Cap5E, putative
capsular polysaccharide biosynthesis protein Cap5E
capsular polysaccharide synthesis enzyme Cap8F
capsular polysaccharide biosynthesis protein Cap8F
capsular polysaccharide synthesis enzyme O-acetyl transferase Cap5H, putative
capsular polysaccharide biosynthesis protein O-acetyl transferase Cap5H
capsular polysaccharide biosynthesis protein Cap5I, putative
capsular polysaccharide biosynthesis protein Cap5I
capsular polysaccharide biosynthesis protein Cap5J, putative
capsular polysaccharide biosynthesis protein Cap5J
capsular polysaccharide biosynthesis protein Cap5K, putative
capsular polysaccharide biosynthesis protein Cap5K
cap5L protein/glycosyltransferase, putative
capsular polysaccharide biosynthesis protein Cap8M
cap5N protein/UDP-glucose 4-epimerase, putative
cap5O protein/UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase
iron regulated; catalyzes the release of heme from hemoglobin allowing bacterial pathogens to use the host heme as an iron source
Catalyzes the reversible two-electron oxidation of formate to carbon dioxide. It allows the assimilation of carbon dioxide and provides energy for growth through oxidative phosphorylation coupled to the reduction of oxygen, nitrate, sulphate or fumarate
bifunctional arginine biosynthesis protein ArgJ; functions at the 1st and 5th steps in arginine biosynthesis; involved in synthesis of acetylglutamate from glutamate and acetyl-CoA and ornithine by transacetylation between acetylornithine and glutmate
bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis and the reduction of N-acetyl-gamma-aminoadipyl-phosphate to N-acetyl-L-aminoadipate-semialdehyde in lysine biosynthesis; involved in both the arginine and lysine biosynthetic pathways; lysine is produced via the AAA pathway, lysine from alpha-aminoadipate
N-acetyl-gamma-glutamyl-phosphate reductase
branched-chain amino acid transport system II carrier protein
catalyzes the cleavage of the lactyl ether moiety of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate; involved in MurNAc dissimilation pathway
N-acetylmuramic acid-6-phosphate etherase
type I site-specific deoxyribonuclease, HsdR family, putative
HsdR family type I site-specific deoxyribonuclease
peptide ABC transporter, ATP-binding protein, putative
peptide ABC transporter ATP-binding protein
peptide ABC transporter, permease protein, putative
FMN-dependent; requires NADH; catalyzes the cleavage of azo bond in aromatic azo compounds
M23/M37 peptidase domain-containing protein
multiple sugar-binding transport ATP-binding protein, putative
multiple sugar-binding transport ATP-binding protein
bacterial extracellular solute-binding protein, putative
maltose ABC transporter, permease protein, putative
maltose ABC transporter, permease protein
cytoplasmic membrane protein that functions as a monomer; catalyzes the active transport of sugar-phosphates such as glucose-6-phosphate with the obligatory exchange of inorganic phosphate or organophosphate
Response regulator receiver domain protein
response regulator receiver domain-containing protein
pyruvate formate-lyase 1 activating enzyme, putative
pyruvate formate-lyase 1 activating enzyme
acyl CoA:acetate/3-ketoacid CoA transferase
PTS system, glucose-specific IIBC component, putative
PTS system glucose-specific transporter subunit IIBC
phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2, putative
4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; forms homodimers
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
teichoic acid biosynthesis protein F, putative
two-component sensor histidine kinase, putative
negatively regulates murein hydrolase activity
in conjunction with LrgA this protein inhibits the expression or activity of extracellular murein hydrolases
cytoplasmic mutarotase that catalyzes the conversion between beta-pyran and beta-furan forms of D-ribose; RbsD is required for efficient ribose utilization in E. coli; rbsD-mutant E. coli strains are unable to use ribose as a sole carbon source
5'-nucleotidase, lipoprotein e(P4) family
ABC transporter, ATP-binding protein, putative
pfkB family carbohydrate kinase family, putative
catalyzes the formation of pyruvate and N-acetylmannosamine from N-acetylneuraminic acid
Converts N-acetylmannosamine-6-phosphate to N-acetylglucosamine-6-phosphate
N-acetylmannosamine-6-phosphate 2-epimerase
membrane component; functions with enzymes IIB (sgaB; ulaB) and IIA (sgaA; ulaC) enzyme I and HPr for anaerobic utilization and uptake of L-ascorbate; sgaTBA are regulated by yifQ as well as Crp and Fnr
PTS system ascorbate-specific transporter subunit IIC
ribosomal-protein-serine acetyltransferase, putative
50S ribosomal protein L7 serine acetyltransferase
mttA/Hcf106 family protein-related protein
catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine
5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
catalyzes the formation of 5,10-methylenetetrahydrofolate from 5-methyltetrahydrofolate and S-adenosyl-L-homocysteine and methionine from S-adenosyl-L-methionine and L-homocysteine; expressed in B. subtilis under methionine starvation conditions
bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
chromosome partioning protein, ParB family, putative
ParB family chromosome partioning protein
translation-associated GTPase; the crystal structure of the Haemophilus influenzae YchF protein showed similarity to the yeast structure (PDB: 1NI3); fluorescence spectroscopy revealed nucleic acid binding; the yeast protein YBR025c interacts with the translation elongation factor eEF1
GTP-dependent nucleic acid-binding protein EngD
binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21
bacteriophage L54a, single-stranded DNA binding protein
bacteriophage L54a single-stranded DNA binding protein
binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit
alkyl hydroperoxide reductase, subunit F, putative
Catalyzes the transfer of the phosphoribosyl moiety from 5-phospho--D-ribosyl-1-pyrophosphate (PRib-PP) to the 6-oxo-guanine and -xanthine
contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
these proteins share structural homology to known superantigen proteins but do not exhibit any of the properties expected such as histocompatibility complex class II binding or broad T-cell activation
SSL5, SET3, SET10; bind P-selectin glycoprotein ligand-1 and inhibit P-selectin-mediated neutrophil rolling along the endothelium; these proteins share structural homology to known superantigens but do not exhibit any of the properties expected such as histocompatibility complex class II binding or broad T-cell activation
SSL7, SET1; blocks IgA-FcR interactions and inhibits complement, leading to increased survival of a sensitive bacterium in blood
type I restriction-modification system, M subunit
type I restriction-modification system subunit M
restriction modification system specificity subunit, putative
restriction modification system specificity subunit
Catalyzes the transfer of electrons from NADH to ubiquinone
trans-sulfuration enzyme family protein, putative
ABC transporter, permease protein, putative
ABC transporter, substrate-binding protein, putative
ABC transporter substrate-binding protein
transcriptional regulator, lysR family, putative
glutamate synthase, large subunit, putative
glutamate synthase is composed of subunits alpha and beta; beta subunit is a flavin adenine dinucleotide-NADPH dependent oxidoreductase; provides electrons to the alpha subunit, which binds L-glutamine and 2-oxoglutarate and forms L-glutamate
DNA polymerase III, gamma and tau subunits, putative
DNA polymerase III subunits gamma and tau
involved in a recombinational process of DNA repair, independent of the recBC complex
catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP)
DNA polymerase III, delta prime subunit, putative
in Bacillus subtilis this protein is involved in the negative regulation of DNA replication initiation; interacts with DnaN and DnaA
DNA replication intiation control protein YabA
methionine--tRNA ligase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content
catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin
An essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis
4-diphosphocytidyl-2C-methyl-D-erythritol kinase
stage V sporulation protein G; essential for spore formation and a negative regulator of asymmetric septation in Bacillus; involved in methicillin-resistance, biofilm formation and capsular polysaccharide synthesis in Staphylococcus
forms a homotrimer; catalyzes the acetylation of glucosamine-1-phosphate and uridylation of N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc; function in cell wall synthesis
bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
catalyzes the formation of 5-phospho-alpha-D-ribose 1-phosphate from D-ribose 5-phosphate and ATP
the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response
50S ribosomal protein L25/general stress protein Ctc
Enables the recycling of peptidyl-tRNAs produced at termination of translation
becomes active under oxidative stress; four conserved cysteines bind a zinc atom when they are in the reduced state and the enzyme is inactive; oxidative stress results in oxidized cysteines, release of zinc, and binding of Hsp33 to aggregation-prone proteins; forms dimers and higher order oligomers
2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase
class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1
with PdxT forms pyridoxal 5'-phosphate from glutamine, either ribose 5-phosphate or ribulose 5-phosphate, and either glyceraldehyde 3-phosphate or dihydroxyacetone phosphate
with PdxST is involved in the biosynthesis of pyridoxal 5'-phosphate; PdxT catalyzes the hydrolysis of glutamine to glutamate and ammonia; PdxS utilizes the ammonia to synthesize pyridoxal 5'-phosphate
ATP:guanido phosphotransferase, C-terminal catalytic domain protein
Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents
Charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation
catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB
forms a complex with SecY and SecG; SecYEG forms a putative protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force
transcription antitermination protein, putative
in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA
binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit
present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme
DNA-directed RNA polymerase subunit beta
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit beta' binds to sigma factor allowing it to bind to the -10 region of the promoter
DNA-directed RNA polymerase subunit beta'
in Bacillus subtilis this non-essential protein associates with the ribosome
interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance
binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit
EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene
EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu
catalyzes the formation of 2-amino-3-oxobutanoate from acetyl-CoA and glycine
2-amino-3-ketobutyrate coenzyme A ligase
Hsp31 stabilizes early unfolding protein intermediates under severe heat stress
catalyzes the phosphorylation of ribulose to ribulose 5-phosphate
catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids
branched-chain amino acid aminotransferase
haloacid dehalogenase-like hydrolase, putative
similar protein in Methanocaldococcus converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate as the first step in methanopterin biosynthesis
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
in Salmonella this enzyme is required for ethanolamine catabolism; has higher affinity for CoA than Pta
similar to zinc-dependent eukaryotic ADH enzymes and distinct from fermentative ADHs
catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase
iron compound ABC transporter, substrate-binding protein, putative
iron compound ABC transporter substrate-binding protein
staphylococcal accessory regulator T, putative
subunit A of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
monovalent cation/H+ antiporter subunit A
subunit B of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
monovalent cation/H+ antiporter subunit B
subunit C of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
monovalent cation/H+ antiporter subunit C
subunit D of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; contains an oxidoreductase domain; catalyzes the transfer of electrons from NADH to ubiquinone; in S. meliloti it is known to be involved specifically with K+ transport
monovalent cation/H+ antiporter subunit D
subunit E of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; in S. meliloti it is known to be involved specifically with K+ transport
monovalent cation/H+ antiporter subunit E
subunit G of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
monovalent cation/H+ antiporter subunit G
iron (chelated) ABC transporter, permease protein, putative
iron (chelated) ABC transporter permease
with TagG is involved in the export of teichoic acids
teichoic acids export protein ATP-binding subunit
teichoic acid biosynthesis protein, putative
glycerol-3-phosphate cytidylyltransferase
iron compound ABC transporter, ATP-binding protein
iron compound ABC transporter ATP-binding protein
ferrichrome transport permease fhuB, putative
ferrichrome ABC transporter (permease), putative
with DhaL and DhaM forms dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone; DhaK is the dihydroxyacetone binding subunit of the dihydroxyacetone kinase
PTS system mannnose-specific family transporter subunit IIA
ABC transporter ATP-binding protein, putative
major facilitator superfamily protein superfamily
BacA; phosphatase activity in Escherichia coli not kinase; involved in bacitracin resistance as bacitracin supposedly sequesters undecaprenyl disphosphate which reduces the pool of lipid carrier available to the cell
deoxyribodipyrimidine photolyase, putative
quinolone resistance norA protein, putative
N-acetylglucosamine-6-phosphate deacetylase
7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE
6-pyruvoyl tetrahydropterin synthase superfamily, putative
para-aminobenzoate synthase, glutamine amidotransferase, component II, putative
para-aminobenzoate synthase glutamine amidotransferase component II
annotated as putative para-aminobenzoate synthase, component I containing frameshift in strain Col
amino acid ABC transporter, permease protein, putative
catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis
NADPH-dependent; catalyzes the reduction of 7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in queuosine biosynthesis
in Salmonella NrdI has a stimulatory effect on the ribonucleotide reductase activity of NrdH with NrdEF
ribonucleotide reductase stimulatory protein
Catalyzes the rate-limiting step in dNTP synthesis
ribonucleotide-diphosphate reductase subunit alpha
B2 or R2 protein; type 1b enzyme; catalyzes the rate-limiting step in dNTP synthesis; converts nucleotides to deoxynucleotides; forms a homodimer and then a multimeric complex with NrdE
ribonucleotide-diphosphate reductase subunit beta
catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis
UDP-N-acetylenolpyruvoylglucosamine reductase
catalyzes the release of the N-terminal amino acid from a tripeptide
functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins
peptide chain release factor 2, putative
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate
catalyzes the phosphorylation of the phosphocarrier protein HPr of the bacterial phosphotransferase system
transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein
prolipoprotein diacylglyceryl transferase
hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
ATP-dependent Clp protease proteolytic subunit
glyceraldehyde-3-phosphate dehydrogenase, type I
glyceraldehyde-3-phosphate dehydrogenase
Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway
Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate
catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
enolase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis
binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation
extracellular matrix and plasma binding protein, putative
extracellular matrix and plasma binding protein
catalyzes the dehydration of 3-dehydroquinate to form 3-dehydroshikimate in aromatic amino acid biosynthesis
part of multienzyme complex composed of H, L, P, and T proteins which catalyzes oxidation of glycine to yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a reduced pyridine nucleotide; protein H is involved in transfer of methylamine group from the P to T protein; covalently bound to a lipoyl cofactor
aminotransferase, class V superfamily, putative
catalyzes the radical-mediated insertion of two sulfur atoms into an acyl carrier protein (ACP) bound to an octanoyl group to produce a lipoyl group
D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain protein
D-isomer specific 2-hydroxyacid dehydrogenase NAD binding domain-containing protein
transfers D-alanine to the D-alanyl carrier protein during the incorporation of D-alanine into lipoteichoic acid
D-alanine--poly(phosphoribitol) ligase subunit 1
D-alanyl carrier protein subunit; involved in the incorporation of D-alanine into membrane-associated D-alanyl-lipoteichoic acid; D-alanyl carrier protein is the acceptor of activated D-alanine which it donates to a membrane acceptor(D-alanyl transferase) for incorporation into membrane lipoteichoic acid
D-alanine--poly(phosphoribitol) ligase subunit 2
subunit F of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
monovalent cation/H+ antiporter subunit F
subunit E of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali
subunit D of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; contains an oxidoreductase domain; catalyzes the transfer of electrons from NADH to ubiquinone
subunit B of antiporter complex involved in resistance to high concentrations of Na+, K+, Li+ and/or alkali; in the case of S. meliloti it was proved to be involved specifically with K+ transport
peptidyl-prolyl cis-trans isomerase, cyclophilin-type, putative
cyclophilin-type peptidyl-prolyl cis-trans isomerase
glutamate dehydrogenase, NAD-specific, putative
catalyzes the formation of arginine from (N-L-arginino)succinate
catalyzes the formation of 2-N(omega)-(L-arginino)succinate from L-citrulline and L-aspartate in arginine biosynthesis, AMP-forming
functions in sugar metabolism in glycolysis and the Embden-Meyerhof pathways (EMP) and in gluconeogenesis; catalyzes reversible isomerization of glucose-6-phosphate to fructose-6-phosphate; member of PGI family
ATP-dependent nuclease subunit A, putative
NADPH-dependent; catalyzes the reduction of coenzyme A disulfide
ATP-dependent Clp protease, ATP-binding subunit ClpB
ATP-dependent Clp protease ATP-binding subunit ClpB
FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs
oligopeptide ABC transporter, ATP-binding protein, putative
oligopeptide ABC transporter ATP-binding protein
oligopeptide ABC transporter, substrate-binding protein, putative
oligopeptide ABC transporter substrate-binding protein
oligopeptide ABC transporter, permease protein, putative
catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
the anti-alpha factor Spx interacts with RNA polymerase alpha subunit C-terminal domain in a region that interacts with the sigma 70 subunit and may interfere with activation of promoters; in Bacillus subtilis this protein is a substrate for ClpXP protease; blocks transcription of the competence regulatory gene encoded by the srf operon; regulates a number of genes involved in thiol homeostasis including trxA and trxB; monomeric member of ArsC family of proteins; does not bind DNA; contains a disulfide bond between C10 and C13 which may sense disulfide stress
enables recognition and targeting of proteins for proteolysis, involved in negative regulation of competence
catalyzes the phosphorylation of NAD to NADP
Catalyzes a key regulatory step in fatty acid biosynthesis
processive; catalyzes the formation of mono-, di- and tri-glucosyldiacylglycerol by the progressive transfer of glucosyl residues to diacylglycerol; involved in the formation of membrane glycolipids
involved in cell wall formation; peptidoglycan synthesis; cytoplasmic enzyme; catalyzes the addition of lysine to UDP-N-acetylmuramoyl-L-alanyl-D-glutamate forming UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine
UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase
stimulates the release of release factors 1 and 2 from the ribosome after hydrolysis of the ester bond in peptidyl-tRNA has occurred; GDP/GTP-binding protein
lipoyltransferase and lipoate-protein ligase, putative
lipoyltransferase and lipoate-protein ligase
catalyzes the formation of dimethylmenaquinone from 1,4-dihydroxy-2-naphthoate and octaprenyl diphosphate
1,4-dihydroxy-2-naphthoate octaprenyltransferase
2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase
2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
catalyzes the formation of 1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA
glutamyl endopeptidase precursor, putative
transcriptional regulator, MarR family, putative
bifunctional autolysin precursor, putative
cytochrome c oxidase subunit III, putative
quinol oxidase AA3, subunit II, putative
catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase
phosphoribosylaminoimidazole carboxylase, catalytic subunit
5-(carboxyamino)imidazole ribonucleotide mutase
With PurE catalyzes the conversion of aminoimidazole ribonucleotide to carboxyaminoimidazole ribonucleotide in the de novo purine nucleotide biosynthetic pathway
5-(carboxyamino)imidazole ribonucleotide synthase
catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase
phosphoribosylaminoimidazole-succinocarboxamide synthase
phosphoribosylformylglycinamidine synthase, PurS protein
phosphoribosylformylglycinamidine synthase PurS
catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis
phosphoribosylformylglycinamidine synthase I
phosphoribosylformylglycinamidine synthase II
Catalyzes first step of the de novo purine nucleotide biosynthetic pathway
catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis
phosphoribosylglycinamide formyltransferase, putative
phosphoribosylglycinamide formyltransferase
bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis
phosphoenolpyruvate-protein phosphotransferase
cytochrome d ubiquinol oxidase, subunit I, putative
cytochrome d ubiquinol oxidase subunit I
cytochrome d ubiquinol oxidase, subunit II, putative
cytochrome d ubiquinol oxidase subunit II
cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+)
pyruvate dehydrogenase complex, E1 component, alpha subunit, putative
pyruvate dehydrogenase complex, E1 component subunit alpha
pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase beta subunit, putative
pyruvate dehydrogenase complex, E1 component subunit beta
dihydrolipoamide S-acetyltransferase component of pyruvate dehydrogenase complex E2, putative
branched-chain alpha-keto acid dehydrogenase subunit E2
E3 component of pyruvate complex; catalyzes the oxidation of dihydrolipoamide to lipoamide
spermidine/putrescine ABC transporter, permease protein, putative
spermidine/putrescine ABC transporter permease
spermidine/putrescine ABC transporter, spermidine/putrescine-binding protein, putative
spermidine/putrescine ABC transporter substrate-binding protein
inositol monophosphatase family protein, putative
depletion of this protein in Bacillus subtilis results in defects in cell morphology; crystal structure of Staphylococcus protein shows homodimer; putative ligand binding protein
biotin-containing enzyme that catalyzes a two step carboxylation of pyruvate to oxaloacetate
converts protoheme IX and farnesyl diphosphate to heme O
glycerophosphoryl diester phosphodiesterase, putative
glycerophosphoryl diester phosphodiesterase
Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA
iron compound ABC transporter, permease protein, putative
iron compound ABC transporter, permease protein
catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily
phenylalanyl-tRNA synthetase subunit alpha
catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily
phenylalanyl-tRNA synthetase subunit beta
An endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids
DNA-dependent DNA polymerase beta chain, putative
MutS2; MutS-II; involved in blocking homologous and homeologous recombination; has ATPase activity stimulated by recombination intermediates; inhibits DNA strand exchange
recombination and DNA strand exchange inhibitor protein
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
succinate dehydrogenase cytochrome b-558 subunit, putative
succinate dehydrogenase cytochrome b-558 subunit
part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol
succinate dehydrogenase flavoprotein subunit
part of four member succinate dehydrogenase enzyme complex that forms a trimeric complex (trimer of tetramers); SdhA/B are the catalytic subcomplex and can exhibit succinate dehydrogenase activity in the absence of SdhC/D which are the membrane components and form cytochrome b556; SdhC binds ubiquinone; oxidizes succinate to fumarate while reducing ubiquinone to ubiquinol; the catalytic subunits are similar to fumarate reductase
succinate dehydrogenase iron-sulfur subunit
converts L-glutamate to D-glutamate, a component of peptidoglycan
hydrolyzes non-standard nucleotides such as xanthine and inosine
FLIPr; secreted protein that blocks the formyl peptide receptor-like 1 (FPRL1) found in neutrophils, monocytes, B cells, and NK cells; inhibits the binding of chemoattractants (such as formylated peptides) to FPRL1, which initiates the phagocyte mobilization towards the infection site
formyl peptide receptor-like 1 inhibitory protein
catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation
catalyzes the reversible synthesis of carbamate and ATP from carbamoyl phosphate and ADP
MraZ; UPF0040; crystal structure shows similarity to AbrB
16S rRNA (cytosine(1402)-N(4))-methyltransferase
First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
phospho-N-acetylmuramoyl-pentapeptide- transferase
UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function
IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme
lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis
regulates pyrimidine biosynthesis by binding to the mRNA of the pyr genes, also has been shown to have uracil phosphoribosyltransferase activity
bifunctional pyrimidine regulatory protein PyrR/uracil phosphoribosyltransferase
catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis
aspartate carbamoyltransferase catalytic subunit
catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis
catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers
carbamoyl phosphate synthase small subunit
four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity
carbamoyl phosphate synthase large subunit
type 1 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase
involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate
fibrinogen-binding protein A-like protein
Essential for recycling GMP and indirectly, cGMP
Promotes RNA polymerase assembly; latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits
DNA-directed RNA polymerase subunit omega
phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
16S rRNA (cytosine(967)-C(5))-methyltransferase
23S rRNA m2A2503 methyltransferase; methylates the C2 position of the A2530 nucleotide in 23S rRNA; may be involved in antibiotic resistance
23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
catalyzes branch migration in Holliday junction intermediates
negative regulator of genes involved in fatty acid and phospholipid biosynthesis for gram positive bacteria
fatty acid biosynthesis transcriptional regulator
involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY
glycerol-3-phosphate acyltransferase PlsX
malonyl CoA-acyl carrier protein transacylase
3-oxoacyl-(acyl-carrier-protein) reductase, putative
3-oxoacyl-(acyl-carrier-protein) reductase
carries the fatty acid chain in fatty acid biosynthesis
cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity
signal recognition particle-docking protein FtsY
binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity
Essential for efficient processing of 16S rRNA
methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA
this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site
essential GTPase; functions in ribosome assembly; binds a unique part of the 23S rRNA; interacts with ribosomal protein L25(Ctc)
RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids
catalyzes the interconversion of succinyl-CoA and succinate
Catalyzes the only substrate-level phosphorylation in the TCA cycle
catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining, maintains net negative superhelicity
TrmFO; Gid; glucose-inhibited division protein; similar to GidA; the gene from Bacillus subtilis encodes a tRNA-methyltransferase that utilizes folate as the carbon donor and bound flavin as reductant; modifies tRNA at position 54 (uridine) of the T-psi loop to form a C5-methyluridine
heat shock protein involved in degradation of misfolded proteins
ATP-dependent protease peptidase subunit
ATP-dependent protease ATP-binding subunit HslU
CodY; DNA-binding protein that represses the expression of many genes that are induced as cells make the transition from rapid exponential growth to stationary phase (By similarity). It is a GTP-binding protein that senses the intracellular GTP concentration as an indicator of nutritional limitations. At low GTP concentration it no longer binds GTP and stop to act as a transcriptional repressor
EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu
Catalyzes the phosphorylation of UMP to UDP
Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs
catalyzes the formation of undecaprenyl pyrophosphate from isopentenyl pyrophosphate
catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro)
catalyzes DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time; required for leading strand synthesis; PolC exhibits 3' to 5' exonuclease activity
in Streptococcus pneumoniae this gene was found to be essential; structure determination of the Streptococcus protein shows that it is similar to a number of other proteins
modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination
Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock
catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs
primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence
polyribonucleotide nucleotidyltransferase, putative
polynucleotide phosphorylase/polyadenylase
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
competence-damage inducible protein cinA
catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs
protein from Staphylococcus aureus has phosphodiesterase activity against 2'-3'-cAMP and 2'-3'-cGMP
catalyzes the coenzyme A dependent formation of succinyl-CoA from 2-oxoglutarate and ferredoxin
2-oxoglutarate ferredoxin oxidoreductase subunit beta
catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A)
(dimethylallyl)adenosine tRNA methylthiotransferase
This protein performs the mismatch recognition step during the DNA repair process
This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. Promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex
glycerol uptake operon antiterminator regulatory protein, putative
glycerol uptake operon antiterminator regulatory protein
Converts glycerol and ADP to glycerol-3-phosphate and ADP
aerobic glycerol-3-phosphate dehydrogenase
hydrolase, alpha/beta fold family domain protein
hydrolase alpha/beta fold domain-containing protein
IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity
tRNA delta(2)-isopentenylpyrophosphate transferase
Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase domain protein
catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP; lysine and threonine sensitive
catalyzes the formation of L-aspartate 4-semialdehyde from L-homoserine
catalyzes the formation of L-threonine from O-phospho-L-homoserine
catalyzes the formation of O-phospho-L-homoserine from L-homoserine in threonine biosynthesis from asparate
in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif
located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group do not appear to have the zinc-binding motif
guanosine 5'-monophosphate oxidoreductase
Represses a number of genes involved in the response to DNA damage
large-conductance mechanosensitive channel
Catalyzes the conversion of citrate to isocitrate
decatenates newly replicated chromosomal DNA and relaxes positive and negative DNA supercoiling
sodium:alanine symporter family protein, putative
this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form; provides protection against oxidative stress
catalyzes the formation of 4-hydroxyphenylpyruvate from prephenate
with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine
anthranilate synthase component II, putative
Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate
involved in tryptophan biosynthesis; amino acid biosynthesis; converts 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide and water
catalyzes the formation of 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate from N-(5-phospho-beta-D-ribosyl)-anthranilate in tryptophan biosynthesis
N-(5'-phosphoribosyl)anthranilate isomerase
catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate
catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis
methicillin resistance factor, FemA, putative
oligopeptide transporter putative membrane permease domain
ATP-binding protein; PstABCS is an ATP dependent phosphate uptake system which is responsible for inorganic phosphate uptake during phosphate starvation
phosphate transporter ATP-binding protein
phosphate ABC transporter, permease protein, putative
phosphate ABC transporter, periplasmic phosphate-binding protein, putative
phosphate ABC transporter substrate-binding protein
catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP, in Bacillus, lysine sensitive; regulated by response to starvation.
catalyzes the formation of dihydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis
4-hydroxy-tetrahydrodipicolinate synthase
catalyzes the reduction of 2,3-dihydrodipicolinate to 2,3,4,5-tetrahydrodipicolinate in lysine and diaminopimelate biosynthesis
4-hydroxy-tetrahydrodipicolinate reductase
2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase, putative
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase
catalyzes the hydrolysis of acylphosphate
component of 2-oxoglutarate dehydrogenase complex; catalyzes the transfer of succinyl coenzyme A to form succinyl CoA as part of the conversion of 2-oxoglutarate to succinyl-CoA
SucA; E1 component of the oxoglutarate dehydrogenase complex which catalyzes the formation of succinyl-CoA from 2-oxoglutarate; SucA catalyzes the reaction of 2-oxoglutarate with dihydrolipoamide succinyltransferase-lipoate to form dihydrolipoamide succinyltransferase-succinyldihydrolipoate and carbon dioxide
2-oxoglutarate dehydrogenase E1 component
DNA-binding response regulator, putative
UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis
undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase
phosphotransferase system enzyme IIA, putative
this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; provides protection against oxidative stress
ThyA; catalyzes formation of dTMP and 7,8-dihydrofolate from 5,10-methylenetetrahydrofolate and dUMP; involved in deoxyribonucleotide biosynthesis; there are 2 copies in some Bacilli, one of which appears to be phage-derived
catalyzes the formation of 2-oxobutanoate from L-threonine; catabolic
functions in homologous recombination, DNA repair, and chromosome segregation; binds preferentially to three- and four-stranded DNA intermediates; introduces specific nick sites in four-stranded DNA substrates; functions similarly to Escherichia coli RuvC
catalyzes a two-step reaction, first charging an asparagine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
DnaQ family exonuclease/DinG family helicase, putative
DnaQ family exonuclease/DinG family helicase
bifunctional biotin operon repressor/biotin--[acetyl-CoA-carboxylase] synthetase BirA
catalyzes the addition and repair of the 3'-terminal CCA sequence in tRNA; these proteins belong to the CCA-adding enzyme subfamily 2 which does not have phosphohydrolase activity
catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
3-phosphoshikimate 1-carboxyvinyltransferase
catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis
catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis
catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate
heptaprenyl diphosphate syntase component II, putative
heptaprenyl diphosphate syntase component II
Catalyzes the carbon methylation reaction in the biosynthesis of ubiquinone
ubiquinone/menaquinone biosynthesis methyltransferase
catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate
NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains
in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins
Catalyzes the formation of (d)CDP from ATP and (d)CMP
ATP-dependent DNA helicase RecQ, putative
phage tail tape meausure protein, TP901 family, core region domain protein
bacteriophage L54a, bacterial Ig-like domain group 2 family protein
bacteriophage L54aIg-like domain-containing protein
phage terminase, large subunit, putative
bacteriophage L54a, HNH endonuclease family protein
bacteriophage L54a HNH endonuclease family protein
transcriptional activator rinB-related protein
transcriptional activator rinB-like protein
bacteriophage L54a, deoxyuridine 5-triphosphate nucleotidohydrolase
bacteriophage L54a deoxyuridine 5-triphosphate nucleotidohydrolase
helix-turn-helix domain-containing protein
staphylococcal respiratory response protein SrrB, putative
ribosomal large subunit pseudouridine synthase B, putative
ribosomal large subunit pseudouridine synthase B
transcriptional regulator, Fur, putative
catalyzes the formation of D-glucono-1,5-lactone 6-phosphate from D-glucose 6-phosphate
maltose operon transcriptional repressor
catalyzes the formation of D-ribulose 5-phosphate from 6-phospho-D-gluconate
2-oxoisovalerate dehydrogenase, E2 component, dihydrolipoamide acetyltransferase, putative
2-oxoisovalerate dehydrogenase, E2 component, dihydrolipoamide acetyltransferase
2-oxoisovalerate dehydrogenase, E1 component, beta subunit, putative
2-oxoisovalerate dehydrogenase, E1 component subunit beta
2-oxoisovalerate dehydrogenase, E1 component, alpha subunit, putative
2-oxoisovalerate dehydrogenase, E1 component subunit alpha
regulates arginine biosynthesis when complexed with arginine by binding at site that overlap the promotors of the arginine biosynthesis genes
catalyzes the bidirectional exonucleolytic cleavage of DNA
bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides
Regulates rRNA biosynthesis by transcriptional antitermination
transcription antitermination protein NusB
an AccC homodimer forms the biotin carboxylase subunit of the acetyl CoA carboxylase, an enzyme that catalyzes the formation of malonyl-CoA, which in turn controls the rate of fatty acid metabolism
acetyl-CoA carboxylase biotin carboxylase subunit
acetyl-CoA carboxylase, biotin carboxyl carrier protein
acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA
acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 1 to form the P protein
acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine; forms a heterodimer with subunit 2 to form the P protein
catalyzes the transfer of a methylene carbon from the methylamine-loaded GcvH protein to tetrahydrofolate, causing the release of ammonia and the generation of reduced GcvH protein
glycine cleavage system aminomethyltransferase T
Assists in DNA repair by cleaving phosphodiester bonds at apurinic or apyrimidinic sties to produce new 5' ends that are base-free deoxyribose 5-phosphate residues
sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; primary sigma factor of bacterium
Catalyzes a two-step reaction, first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome
a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA
in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase
16S rRNA (uracil(1498)-N(3))-methyltransferase
methylates ribosomal protein L11 at multiple amino acid positions; mutations of these genes in Escherichia coli or Thermus thermophilus has no apparent phenotype
chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion
heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria
with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor
heat-inducible transcription repressor HrcA
catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III
binds to the ribosome on the universally-conserved alpha-sarcin loop
binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase
required for the assembly and function of the DNAX complex which is required for the assembly of the beta subunit onto primed DNA
DNA internalization-related competence protein ComEC/Rec2
nicotinate (nicotinamide) nucleotide adenylyltransferase
in Bacillus subtilis this enzyme appears to be involved in 30S ribosomal RNA subunit biogenesis
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
acetyl-CoA carboxylase, biotin carboxylase, putative
acetyl-CoA carboxylase biotin carboxylase
acetyl-CoA carboxylase, biotin carboxyl carrier protein, putative
necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
functions in pyrimidine salvage; pyrimidine ribonucleoside kinase; phosphorylates nucleosides or dinucleosides to make UMP or CMP using ATP or GTP as the donor
similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function
Holliday junction resolvase-like protein
Catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes
catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG
hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr); possible defense mechanism against a harmful effect of D-tyrosine
catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis
part of the preprotein secretory system; forms a complex with protein YajC; SecDFyajC stimulates the proton motive force-driven protein translocation, seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF
bifunctional preprotein translocase subunit SecD/SecF
Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr)
Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration
plays an essential role in ATP-dependent branch migration of the Holliday junction
essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication
involved in the peptidyltransferase reaction during translation
in some organisms this protein is a transmembrane protein while in others it is periplasmic; involved in some organisms with other components of the MreBCD complex and with penicillin binding proteins in the periplasm or cell wall
DESCRIPTION:Mutagenesis in Streptococcus pneumoniae.[18556794]
folylpolyglutamate synthase/dihydrofolate synthase, putative
folylpolyglutamate synthase/dihydrofolate synthase
valine--tRNA ligase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain
Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway
glutamate-1-semialdehyde aminotransferase
catalyzes the formation of porphobilinogen from 5-aminolevulinate
transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis
catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins
binds guanine nucleotides; in Escherichia coli depletion results in defective cell division and filamentation; in Bacillus subtilis this gene is essential
ribosome biogenesis GTP-binding protein YsxC
binds and unfolds substrates as part of the ClpXP protease
ATP-dependent protease ATP-binding subunit ClpX
Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer
binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit
IF-3 has several functions that are required and promote translation initiation including; preventing association of 70S by binding to 30S; monitoring codon-anticodon interactions by promoting disassociation of fMet-tRNA(fMet) from initiation complexes formed on leaderless mRNAs or incorrectly bound noninitiatior tRNAs and complexes with noncanonical start sites; stimulates codon-anticodon interactions at P-site; involved in moving mRNA to the P-site; and in recycling subunits
catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr)
Primosomal protein that may act to load helicase DnaC during DNA replication
glyceraldehyde 3-phosphate dehydrogenase 2
catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis
alkaline phosphatase synthesis transcriptional regulatory protein
Converts isocitrate to alpha ketoglutarate
catalyzes the formation of D-fructose 1,6-bisphosphate from D-fructose 6-phosphate in glycolysis
catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein
acetyl-CoA carboxylase carboxyltransferase subunit alpha
catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer of AccA2D2 subunits
acetyl-CoA carboxylase carboxyltransferase subunit beta
DNA polymerase III, alpha subunit superfamily
DNA polymerase III subunit alpha superfamily protein
catalyzes the opening and hydrolysis of the beta-lactam ring of beta-lactam antibiotics such as penicillins and cephalosporins
AckA utilizes acetate and can acetylate CheY which increases signal strength during flagellar rotation; utilizes magnesium and ATP; also involved in conversion of acetate to aceyl-CoA
antioxidant activity; thioredoxin-dependent thiol peroxidase; forms homodimers in solution; shows substrate specificity to alkyl hydroperoxides; periplasmic protein
Required for the synthesis of the thiazole moiety
acts to negatively regulates ftsZ ring formation by modulating the frequency and position of the ftsZ ring formation
primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination
catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate
1-acyl-sn-glycerol-3-phosphate acyltransferases domain protein
1-acyl-sn-glycerol-3-phosphate acyltransferase domain-containing protein
transglycosylase domain-containing protein
catalyzes the formation of 10-formyltetrahydrofolate from formate and tetrahydrofolate
Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA
catalyzes the formation of 3-deoxy-D-aribino-hept-2-ulosonate 7-phosphate from phosphoenolpyruvate and D-erythrose 4-phosphate and the formation of prephenate from chorismate
bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis
tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine
divalent metal ion-dependent extracellular dipeptidase; able to hydrolyze a broad range of dipeptides but no tri-, tetra-, or larger oligopeptides; differences in the amino acid specificity of the cleavage site varies between species; similar to succinyl-diaminopimelate desuccinylases
ribosomal small subunit pseudouridine synthase A, putative
polysaccharide biosynthesis protein, putative
leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase
RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; beta subunit of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not
riboflavin biosynthesis protein, putative
catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine
arsenical pump membrane protein subfamily
catalyzes the reduction of arsenate to arsenite; also can dephosphorylate tyrosine phosphorylated proteins, aryl phosphates, and acyl phosphates
similar to novel fructose-6-phosphate aldolase from Escherichia coli; enzyme from Methanocaldococcus janaschii shows transaldolase activity
may be involved in chromosome condensation; overexpression in Escherichia coli protects against decondensation by camphor; overexpressing the protein results in an increase in supercoiling
catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase
PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP
o-succinylbenzoic acid--coa ligase, putative
type I restriction-modification enzyme, S subunit, EcoA family, putative
type I restriction-modification system subunit S
epidermin biosynthesis protein EpiC, authentic point mutation
lantibiotic epidermin biosynthesis protein EpiB, putative
lantibiotic epidermin biosynthesis protein EpiB
catalyzes the formation of protoporphyrin IX from protoporphyrinogen IX
protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic
catalyzes the formation of coproporphyrinogen from uroporphyrinogen III
catalyzes the exonucleic cleavage of mRNA yielding nucleioside 5'-phosphates
ribosomal large subunit pseudouridine synthase, RluD subfamily, putative
ribosomal large subunit pseudouridine synthase D
class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle
Phosphotransferase system, EIIC domain protein
PTS system transporter subunit IIC domain-containing protein
ferric uptake regulator-like protein, putative
converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate
ABC transporter, ATP-binding/permease protein, putative
A/G-specific adenine glycosylase, putative
binds RecA and inhibits RecA-mediated DNA strand exchange and ATP hydrolysis and coprotease activities
monofunctional; catalyzes the elongation of glycan strands in cell wall biosynthesis
phi ETA orf 58-like protein-related protein
phage putative head morphogenesis protein, SPP1 gp7 family domain protein
phage terminase, large subunit, PBSX family
PBSX family phage terminase large subunit
PV83 orf 23-like protein-related protein
bacteriophage L54a, DnaB-like helicase family protein
bacteriophage L54a DnaB-like helicase family protein
single-strand DNA-binding protein, putative
phi PVL orf 38-like protein-related protein
phi PV83 orf 12-like protein-related protein
bacteriophage L54a, antirepressor, putative
DNA-binding response regulator VraR, putative
catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn
UDP-N-acetylmuramyl tripeptide synthetase, putative
UDP-N-acetylmuramyl tripeptide synthetase
involved in translesion DNA polymerization with beta clamp of polymerase III; belongs to Y family of polymerases; does not contain proofreading function
RNA methyltransferase, TrmA family, putative
allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA
aspartyl/glutamyl-tRNA amidotransferase subunit B
aspartyl/glutamyl-tRNA amidotransferase subunit A
allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; some Mycoplasma proteins contain an N-terminal fusion to an unknown domain
aspartyl/glutamyl-tRNA amidotransferase subunit C
PcrB-like protein; GGGP synthase; member of prenyltransferases that transfer isoprenoid groups to nonisoprenoid acceptors; functions in form GGGP from glycerol-1-phosphate (G-1-P) and geranylgeranyl pyrophosphate (GGPP); important in lipid metabolism and especially important as the ether linkages in archaea are different than those in bacteria; GGGP synthase lies at the branch point for membrane lipid biosynthesis; cytosolic; T acidophilum protein acts as a homodimer while M thermoautotrophicum protein has been reported to function as a pentamer
geranylgeranylglyceryl phosphate synthase-like protein
Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide
catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NAAD) using either ammonia or glutamine as the amide donor and ATP; ammonia-utilizing enzymes include the ones from Bacillus and Escherichia coli while glutamine-utilizing enzymes include the Mycobacterial one; forms homodimers
catalyzes the formation of 5-phospho-alpha-D-ribose 1-diphosphate and nicotinate from nicotinate D-ribonucleotide and diphosphate
nitric oxide synthase, oxygenase domain, putative
sodium-dependent transporter (huNaDC-1), putative
catalyzes the hydrolysis of pyrophosphate to phosphate
manganese-dependent inorganic pyrophosphatase
secreted protein that specifically inhibits the activation of neutrophils and monocytes by binding to the formylated peptide receptor and the C5a receptor; blocks neutrophil migration towards the infection site and hinders the establishment of the initial defense against the infection
phage minor structural protein, N-terminal region domain protein
phi PVL orf 3-like protein-related protein
phage terminase, small subunit, putative
dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE), catalyzes the hydrolysis of N-succinyl-L,Ldiaminopimelate L,L-SDAP to L,L-diaminopimelate and succinate. It is a metalloprotease containing dinuclear active sites. Its structure is similar to the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 and the aminopeptidase from Aeromonas proteolytica.
60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth
10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring
Lyses erythrocytes and other mammalian cells
sucrose-6-phosphate dehydrogenase, putative
modulates transcription in response to the NADH/NAD(+) redox state
redox-sensing transcriptional repressor Rex
in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity
DNA-binding/iron metalloprotein/AP endonuclease
ribosomal-protein-alanine acetyltransferase
catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis
acetolactate synthase, large subunit, biosynthetic type
with IlvI catalyzes the formation of 2-acetolactate from pyruvate, this subunit subunit is required for full activity and valine sensitivity; also known as acetolactate synthase small
acetolactate synthase 1 regulatory subunit
catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis
catalyzes the formation of 2-isopropylmalate from acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis
catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis
dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate
catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D
3-isopropylmalate dehydratase small subunit
catalyzes the formation of 2-oxobutanoate from L-threonine; biosynthetic
S1 RNA-binding domain-containing protein
sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcribed in the early stationary phase under conditions inimical to sporulation; induced by heat shock, salt stress, oxidative stress, glucose limitation, oxygen limitation and entry into stationary phase
binds to sigma-B preventing the formation of an RNA polymerase holoenzyme
Catalyzes the formation of holo-ACP, which mediates the essential transfer of acyl fatty acid intermediates during the biosynthesis of fatty acids and lipids
one of the components of the high-affinity ATP-driven potassium transport (or KDP)system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions; the C subunit may be involved in assembly of the KDP complex
One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions
catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions
ATP-dependent RNA helicase, DEAD box family, putative
UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli
Condenses 4-methyl-5-(beta-hydroxyethyl)-thiazole monophosphate and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate to form thiamine monophosphate
catalyzes the formation of 4-methyl-5-(2-phosphoethyl)-thiazole and ADP from 4-methyl-5-(2-hydroxyethyl)-thiazole and ATP
catalyzes the formation of 4-amino-2-methyl-5-diphosphomethylpyrimidine
in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP
adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane
Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit
produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit
Produces ATP from ADP in the presence of a proton gradient across the membrane; the delta subunit is part of the catalytic core of the ATP synthase complex
produces ATP from ADP in the presence of a proton gradient across the membrane; subunit B is part of the membrane proton channel
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0
Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate
catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate
modification methylase, HemK family, putative
recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2
catalyzes the formation of thymidine 5'-phosphate from thymidine
RpmE2; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster do not have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome
An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes
catalyzes the formation of glycerone phosphate and glyceraldehyde 3-phosphate from fructose 1,6, bisphosphate; induced by anaerobic conditions in Bacillus subtilis
CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer
participates in both the initiation and recycling phases of transcription; in the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling
DNA-directed RNA polymerase subunit delta
catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type II pantothenate kinases are not regulated by feedback inhibition by coenzyme A
aminobenzoyl-glutamate utilization protein B, putative
aminobenzoyl-glutamate utilization protein B
catalyzes the hydrolysis of S-ribosylhomocysteine to homocysteine and autoinducer-2
Catalyzes the reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway
Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source
glucosamine--fructose-6-phosphate aminotransferase
PTS system, mannitol-specific component, putative
PTS system, mannitol-specific IIa component, putative
PTS system mannitol-specific transporter subunit IIA
mannitol-1-phosphate 5-dehydrogenase, putative
catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
UDP-N-acetylglucosamine pyrophosphorylase, putative
UDP-N-acetylglucosamine pyrophosphorylase
ABC transporter periplasmic binding protein, putative
osmoprotectant transporter, BCCT family, opuD-like protein, putative
catalyzes the formation of 6-phospho-galactose from a 6-phospho-beta-galactoside
pts system, lactose-specific IIBC component, putative
PTS system lactose-specific transporter subunit IIBC
PTS system lactose-specific IIA component, putative
PTS system lactose-specific transporter subunit IIA
catalyzes the reversible reaction of dihydroxyacetone phosphate with glyceraldehyde 3-phosphate to produce tagatose 1,6-bisphosphate; in Streptococcus pyogenes there are two paralogs of tagatose-bisphosphate aldolase, encoded by lacD1 and lacD2; expression of lacD1 is highly regulated by environmental conditions while lacD2 specializes in an efficient utilization of carbohydrate sources
catalyzes the formation of tagatose 1,6-bisphosphate from tagatose 6-phosphate and ATP
catalyzes the interconversion of galactose 6-phosphate to tagatose 6-phosphate
galactose-6-phosphate isomerase subunit LacB
catalyzes the interconversion of galactose 6-phosphate to tagatose 6-phosphate; tagatose pathway for galactose utilization
galactose-6-phosphate isomerase subunit LacA
lactose phosphotransferase system repressor, putative
lactose phosphotransferase system repressor
Modulates the activities of several enzymes which are inactive in their acetylated form
transcriptional regulator, merR family, putative
catalyzes the formation of 2-acetolactate from pyruvate in stationary phase
forms a direct contact with the tRNA during translation
in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit
mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability
with CbiNQ forms the ABC transporter for cobalt import; Staphylococcus has two adjacent copies of this gene
is a component of the macrolide binding site in the peptidyl transferase center
catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme
DNA-directed RNA polymerase subunit alpha
located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3
located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA
smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif
stimulates the activities of the other two initiation factors, IF-2 and IF-3
essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP
forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase
L30 binds domain II of the 23S rRNA and the 5S rRNA; similar to eukaryotic protein L7
located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance
binds 5S rRNA along with protein L5 and L25
ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance
binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group appear to contain the zinc-binding motif
part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13
assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel
binds to the 23S rRNA between the centers for peptidyl transferase and GTPase
primary binding protein; helps mediate assembly; involved in translation fidelity
one of the stabilizing components for the large ribosomal subunit
located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e
forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation
binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center
protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation
binds third domain of 23S rRNA and protein L29; part of exit tunnel
L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA
binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin
decatenates replicating daughter chromosomes
peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB
molybdopterin cofactor biosynthesis protein A, putative
molybdenum cofactor biosynthesis protein A
in Escherichia coli MobA links a guanosine 5'-phosphate to molydopterin to form molybdopterin guanine dinucleotide during molybdenum cofactor biosynthesis
molybdopterin-guanine dinucleotide biosynthesis protein MobA
molybdopterin converting factor, subunit 1
molybdopterin converting factor subunit 1
molybdopterin converting factor moa, putative
molybdopterin-guanine dinucleotide biosynthesis protein MobB
molybdopterin biosynthesis protein moeA, putative
MoaC; along with MoaA is involved in conversion of a guanosine derivative into molybdopterin precursor Z; involved in molybdenum cofactor biosynthesis
molybdenum cofactor biosynthesis protein MoaC
molybdopterin precursor biosynthesis moaB, putative
molybdopterin precursor biosynthesis MoaB
molybdenum transport ATP-binding protein ModC, putative
molybdenum transport ATP-binding protein ModC
molybdenum ABC transporter, permease protein, putative
molybdenum ABC transporter, periplasmic molybdate-binding protein
molybdenum ABC transporter substrate-binding protein
involved in the production or activity of formate dehydrogenase-H which is active when nitrate is not present during anaerobic growth
UreA, with UreB and UreC catalyzes the hydrolysis of urea into ammonia and carbon dioxide; nickel metalloenzyme; accessory proteins UreD, UreE, UreF, and UreG are necessary for assembly of the metallocenter
ureases catalyze the hydrolysis of urea into ammonia and carbon dioxide; in Helicobacter pylori and Yersinia enterocolitica the ammonia released plays a key role in bacterial survival by neutralizing acids when colonizing the gastric mucosa; the holoenzyme is composed of 3 UreC (alpha) and 3 UreAB (gamma/beta); in Staphylococcus saprophyticus plays a role in bladder invasiveness and may induce the formation of kidney and bladder stones due to the alkalization of urine
ureases catalyze the hydrolysis of urea into ammonia and carbon dioxide; in Helicobacter pylori the ammonia released plays a key role in bacterial survival by neutralizing acids when colonizing the gastric mucosa; the holoenzyme is composed of 3 ureC (alpha) and 3 ureAB (gamma/beta) subunits
involved in the assembly of the urease metallocenter; possible nickel donor
secretory antigen precursor SsaA, putative
N-acetylmuramoyl-L-alanine amidase, putative
formate dehydrogenase, alpha subunit, putative
catalyzing the hydrolysis of 4-imidazolone-5-propionate to N-formimidoyl-L-glutamate, the third step in the histidine degradation pathway
catalyzes the formation of 4-imidazolone-5-propanoate from urocanate during histidine metabolism
catalyzes resistance to fosfomycin by the addition of a thiol cofactor
catalyzes the formation of glutamate and formamide from N-formimidoyl-L-glutamate
catalyzes D-ribose 5-phosphate --> D-ribulose 5-phosphate in the nonoxidative branch of the pentose phosphate pathway
DNA-3-methyladenine glycosylase, putative
catalyzes the isomerization of isopentenyl pyrophosphate to dimethylallyl diphosphate
drug resistance transporter, EmrB/QacA subfamily, putative
EmrB/QacA family drug resistance transporter
malate dehydrogenase; catalyzes the oxidation of malate to oxaloacetate
PTS system sucrose-specific IIBC component, putative
PTS system sucrose-specific transporter subunit IIBC
PTS system sucrose-specific IIBC component
respiratory nitrate reductase, gamma subunit
respiratory nitrate reductase subunit gamma
respiratory nitrate reductase, delta subunit, putative
respiratory nitrate reductase subunit delta
uroporphyrin-III C-methyltransferase, putative
assimilatory nitrite reductase [NAD(P)H], small subunit, putative
assimilatory nitrite reductase [NAD(P)H] small subunit
assimilatory nitrite reductase [NAD(P)H], large subunit, putative
assimilatory nitrite reductase [NAD(P)H] large subunit
methicillin resistance determinant protein FmhA
amino acid ABC transproter, permease protein, putative
2,3-bisphosphoglycerate-dependent; catalyzes the interconversion of 2-phosphoglycerate to 3-phosphoglycerate
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase
immunoglobulin G-binding protein Sbi, putative
gamma-hemolysin h-gamma-ii subunit, putative
leukocidin s subunit precursor, putative
catalyzes the formation of pimeloyl-CoA from pimelate and coenzyme A
catalyzes the formation of biotin from dethiobiotin and sulfur 2 S-adenosyl-L-methionine
adenosylmethionine-8-amino-7-oxononanoate aminotransferase
adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
positive transcriptional activator, putative
amino acid ABC transporter-like protein, putative
ketopantoate reductase; catalyzes the NADPH reduction of ketopantoate to pantoate; functions in pantothenate (vitamin B5) biosynthesis
glutamate synthase alpha subunit, putative
nickel ABC transporter, permease protein, putative
peptide ABC transporter, peptide-binding protein, putative
peptide ABC transporter substrate-binding protein
UTP-glucose-1-phosphate uridylyltransferase
fibronectin-binding protein precursor, putative
gluconate operon transcriptional repressor, putative
gluconate operon transcriptional repressor
catalyzes the formation of pyruvate from lactate
L-serine dehydratase, iron-sulfur-dependent, alpha subunit
L-serine dehydratase, iron-sulfur-dependent subunit alpha
L-serine dehydratase, iron-sulfur-dependent, beta subunit
L-serine dehydratase iron-sulfur-dependent subunit beta
PTS system glucose-specific IIABC component
PTS system glucose-specific transporter subunit IIABC
catalyzes the formation of acetyl phosphate from pyruvate
hydroxymethylglutaryl-CoA reductase, degradative
methylated-DNA--protein-cysteine methyltransferase, putative
methylated-DNA--protein-cysteine methyltransferase
ATP-dependent Clp protease, ATP-binding subunit ClpC, putative
ATP-dependent Clp protease ATP-binding subunit ClpC
catalyzes the conversion of 1-proline-5-carboxylate dehydrogenase to L-glutamate
cation-transporting ATPase, E1-E2 family, putative
catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors
Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5)
catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine
catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate
3-methyl-2-oxobutanoate hydroxymethyltransferase
catalyzes the formation of succinate semialdehyde and glutamate from 4-aminobutanoate and 2-oxoglutarate
catalyzes the formation of glycerone phosphate and D-glyceraldehyde 3-phosphate from D-fructose 1,6-bisphosphate in glycolysis
catalyzes the oxidation of choline to betaine aldehyde and betain aldehyde to glycine betaine
Catalyzes the reduction of nucleoside 5'-triphosphates to 2'-deoxynucleoside 5'-triphosphates
anaerobic ribonucleoside triphosphate reductase
catalyzes the formation of siroheme from precorrin-2
sulfite reductase (NADPH) flavoprotein alpha-component, putative
sulfite reductase (NADPH) flavoprotein subunit alpha
nisin susceptibility-associated sensor histidine kinase
nisin susceptibility-associated DNA-binding response regulator
alkaline phosphatase III precursor, putative
catalyzes the formation of ornithine and carbamylphosphate from citrulline in the arginine catabolic pathway
catalyzes the degradation of arginine to citruline and ammonia
PTS system, fructose-specific IIABC component, putative
PTS system fructose-specific transporter subunit IIABC
accessory Sec system glycosylation chaperone GtfB
accessory Sec system glycosyltransferase GtfA
functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; in some organisms, especially gram positive pathogens, have paralogs that have been found to be nonessential but do function in secretion of a subset of exported proteins
accessory Sec system protein translocase subunit SecY2
this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form; a fusion protein of this enzyme with MsrB provides protection against oxidative stress in Neisseria gonorrhoeae
capsular polysaccharide biosynthesis protein, Cap5C
capsular polysaccharide biosynthesis protein Cap5C
capsular polysaccharide biosynthesis, capA, putative
capsular polysaccharide biosynthesis protein CapA
ica operon transcriptional regulator IcaR, putative
ica operon transcriptional regulator IcaR
predicted polysaccharide polymerase involved in biofilm formation; required for the synthesis of the beta-1,6-N-acetylglucosamine polysaccharide; PgaC; in Yersinia the HmsR protein is an inner membrane protein
intercellular adhesion protein C, putative
imidazole glycerol phosphate synthase subunit hisF, putative
multifunctional imidazole glycerol phosphate synthase subunit/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-hosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide
1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide
imidazole glycerol phosphate synthase subunit HisH
catalyzes the dehydration of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to 3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine biosynthesis
histidinol-phosphate aminotransferase, putative
catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer
short form of enzyme; requires HisZ for function; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms heteromultimer of HisG and HisZ
ATP phosphoribosyltransferase catalytic subunit
May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine
ATP phosphoribosyltransferase regulatory subunit
catalyzes the removal of 5-oxoproline from various penultimate amino acid residues except L-proline
high affinity nickel transporter, putative
integrase/recombinase, core domain family
glucose-inhibited division protein B; SAM-dependent methyltransferase; methylates the N7 position of guanosine in position 527 of 16S rRNA
GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs
tRNA uridine 5-carboxymethylaminomethyl modification protein GidA
in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE
protein component of RNaseP which catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'terminus; this enzyme also cleaves other RNA substrates
in Escherichia coli transcription of this gene is enhanced by polyamines