Saccharomyces cerevisiae S288c mitochondrion, complete genome.
mitochondrion Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae
Submitted (14-JAN-2015) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (12-DEC-2014) Genetics, Saccharomyces Genome Database, Stanford University, Stanford, CA 94305, USA
Submitted (16-DEC-1998) Max-Planck-Institut fuer Biochemie, Am Klopferspitz 18a D-82152 Martinsried, Germany
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to KP263414.\nCOMPLETENESS: full length.
EXISTENCE:curator inference:GO:0005739 mitochondrion [PMID:9023104]
EXISTENCE:curator inference:GO:0070125 mitochondrial translational elongation [PMID:9023104]
ORI1; Mitochondrial origin of replication
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:6262728]
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:6262728]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:6262728]
Ribosomal RNA of the small mitochondrial ribosomal subunit; MSU1 allele suppresses ochre stop mutations in mitochondrial protein-coding genes
Mitochondrial tRNA, annotated as tryptophanyl tRNA (tRNA-Trp); predicted by tRNAscan-SE analysis to have specificity for selenocysteine
ORI8; Mitochondrial origin of replication
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9724417]
Subunit I of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits; number of introns in different strains varies from 2 to 8, with most strains having 4-6 introns
EXISTENCE:mutant phenotype:GO:0004519 endonuclease activity [PMID:1314207]
EXISTENCE:mutant phenotype:GO:0006316 movement of group I intron [PMID:1314207]
Endonuclease I-SceIV; involved in intron mobility; encoded by a mobile group I intron within the mitochondrial COX1 gene
intron-encoded DNA endonuclease aI5 alpha
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:7588637]
EXISTENCE:direct assay:GO:0008380 RNA splicing [PMID:7588637]
EXISTENCE:mutant phenotype:GO:0006314 intron homing [PMID:2536592]
Endonuclease I-SceII; encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:7797552]
Endonuclease I-SceIII; encoded by a mobile group I intron within the mitochondrial COX1 gene
EXISTENCE:direct assay:GO:0003964 RNA-directed DNA polymerase activity [PMID:7681727]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7681727]
EXISTENCE:mutant phenotype:GO:0006314 intron homing [PMID:14612420]
Reverse transcriptase required for splicing of the COX1 pre-mRNA; encoded by a mobile group II intron within the mitochondrial COX1 gene
intron-encoded reverse transcriptase aI2
EXISTENCE:direct assay:GO:0006315 homing of group II introns [PMID:11687644]
EXISTENCE:direct assay:GO:0006397 mRNA processing [PMID:11687644]
EXISTENCE:mutant phenotype:GO:0003964 RNA-directed DNA polymerase activity [PMID:15925309]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:15925309]
EXISTENCE:mutant phenotype:GO:0006278 RNA-templated DNA biosynthetic process [PMID:15925309]
intron-encoded reverse transcriptase aI1
hypothetical protein; encoded within an intron of the mitochondrial COX1 gene; translational initiation codon is predicted to be ATA rather than ATG
intron-encoded DNA endonuclease aI5 beta
EXISTENCE:direct assay:GO:0015986 proton movtive force-driven ATP synthesis[PMID:20691145]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:8662204]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis[PMID:8662204]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:8662204]
Subunit 8 of the F0 sector of mitochondrial F1F0 ATP synthase; encoded on the mitochondrial genome; ATP8 and ATP6 mRNAs are not translated in the absence of the F1 sector of ATPase
EXISTENCE:direct assay:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:18722382]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis[PMID:20691145]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis[PMID:17940284|PMID:6251866]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:17940284|PMID:6251866]
Subunit a of the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrially encoded; translation is specifically activated by Atp22p; ATP6 and ATP8 mRNAs are not translated in the absence of the F1 sector of ATPase; mutations in human ortholog MT-ATP6 are associated with neurodegenerative disorders such as Neurogenic Ataxia and Retinitis Pigmentosa (NARP), Leigh syndrome (LS), Charcot-Marie-Tooth (CMT), and ataxia telangiectasia
ORI7; Mitochondrial origin of replication
ORI2; Mitochondrial origin of replication
Mitochondrial glutamate tRNA (tRNA-Glu); predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857|PMID:391561]
EXISTENCE:direct assay:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:391561]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:391561]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:391561]
Cytochrome b; mitochondrially-encoded subunit of ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p; number of introns varies between strains from 0 to 6, with most having 5
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:11142386]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11142386]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2875797]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:11142386]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:8917309]
Mitochondrial mRNA maturase; forms a complex with Nam2p to mediate splicing of the bI4 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA
EXISTENCE:curator inference:GO:0005739 mitochondrion [PMID:2538624]
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:11773622]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11773622]
EXISTENCE:direct assay:GO:0090615 mitochondrial mRNA processing [PMID:11773622]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:2538624]
Mitochondrial mRNA maturase; forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA
EXISTENCE:direct assay:GO:0004518 nuclease activity [PMID:8670880]
EXISTENCE:direct assay:GO:0008380 RNA splicing [PMID:8670880]
Mitochondrial mRNA maturase with a role in splicing; encoded by both exon and intron sequences of partially processed COB mRNA
ORI6; Mitochondrial origin of replication
EXISTENCE:direct assay:GO:0016887 ATPase activity [PMID:18722382]
EXISTENCE:physical interaction:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:2894987]
F0-ATP synthase subunit c (ATPase-associated proteolipid); encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2
Mitochondrial serine tRNA (tRNA-Ser); predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005761 mitochondrial ribosome [PMID:387793]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:24186543|PMID:6250484]
Mitochondrial ribosomal protein of the small subunit; mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane; may have a role in loss of mitochondrial DNA under stress conditions
mitochondrial 37S ribosomal protein VAR1
ORI3; Mitochondrial origin of replication
ORI4; Mitochondrial origin of replication
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:6759872]
EXISTENCE:mutant phenotype:GO:0003735 structural constituent of ribosome [PMID:6759872]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:6759872]
EXISTENCE:mutant phenotype:GO:0042255 ribosome assembly [PMID:6759872]
Mitochondrial 21S rRNA; intron encodes the I-SceI DNA endonuclease
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:16478204|PMID:8335007]
I-SceI DNA endonuclease; encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene
EXISTENCE:curator inference:GO:0070125 mitochondrial translational elongation [PMID:375006]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:375006]
EXISTENCE:direct assay:GO:0030533 triplet codon-amino acid adaptor activity [PMID:375006]
Mitochondrial threonine tRNA (tRNA-Thr); predicted by tRNAscan-SE analysis
EXISTENCE:curator inference:GO:0005739 mitochondrion [PMID:16777356]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:16777356]
Mitochondrial histidine tRNA (tRNA-His); predicted by tRNAscan-SE analysis
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9872396]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:9872396]
Mitochondrial leucine tRNA (tRNA-Leu); predicted by tRNAscan-SE analysis; required for mitochondrial translation and indirectly for maintenance of the mitochondrial genome; mutations in the orthologous human gene cause the neurological disease MELAS
Mitochondrial glutamine tRNA (tRNA-Gln); predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:387075]
Mitochondrial lysine tRNA (tRNA-Lys); predicted by tRNAscan-SE analysis; modification of wobble base U34 is decreased at elevated temperatures, decreasing ability to decode AAG codons; this function is performed at high temperature by imported cytoplasmic tRNA-Lys with CUU anticodon
Mitochondrial arginine tRNA (tRNA-Arg); predicted by tRNAscan-SE analysis
Mitochondrial glycine tRNA (tRNA-Gly); predicted by tRNAscan-SE analysis
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:7024270]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7024270]
Mitochondrial aspartate tRNA (tRNA-Asp); predicted by tRNAscan-SE analysis
Mitochondrial alanine tRNA (tRNA-Ala); predicted by tRNAscan-SE analysis
Mitochondrial isoleucine tRNA (tRNA-Ile); predicted by tRNAscan-SE analysis; mutations in human ortholog are linked to conditions such as progressive encephalopathy, metabolic syndrome, and hypomagnesaemia
Mitochondrial asparagine tRNA (tRNA-Asn); predicted by tRNAscan-SE analysis
Mitochondrial methionine tRNA (tRNA-Met); predicted by tRNAscan-SE analysis
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:224191]
Subunit II of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits
Mitochondrial phenylalanine tRNA (tRNA-Phe); predicted by tRNAscan-SE analysis
EXISTENCE:mutant phenotype:GO:0070125 mitochondrial translational elongation [PMID:375006]
Mitochondrial threonine tRNA (tRNA-Thr); decodes CUA codons as threonine rather than leucine; has an unusual enlarged 8-nt anticodon loop; may be evolutionarily derived from mitochondrial tRNA-His; conserved in some genera of budding yeasts
Mitochondrial valine tRNA (tRNA-Val); predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:7851399|PMID:1331058]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24478450|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:11171120]
Subunit III of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; one of three mitochondrially-encoded subunits
ORI5; Mitochondrial origin of replication
Mitochondrial formylated methionine tRNA (tRNA-fMet); predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0001682 tRNA 5'-leader removal [PMID:3537697]
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:8366116]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11522833]
EXISTENCE:direct assay:GO:0008033 tRNA processing [PMID:11522833]
EXISTENCE:direct assay:GO:0030678 mitochondrial ribonuclease P complex [PMID:3537697]
RNA component of mitochondrial RNase P; mitochondrial RNase P also contains the protein subunit Rpm2p; RNase P removes 5' extensions from mitochondrial tRNA precursors; RPM1 is conserved in bacteria, fungi, and protozoa