Saccharomyces cerevisiae S288C chromosome XVI, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006949.\n\nOn Apr 26, 2011 this sequence version replaced NC_001148.3.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL16L; Telomeric region on the left arm of Chromosome XVI; annotated components include an X element core sequence, X element combinatorial repeats, and a long Y' element; TEL16L does have telomeric repeats (TEL16L-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
Y' element ATP-dependent helicase protein 1 copy 7
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole; identical to Pau21p; encodes two proteins that are translated from two different start codons
EXISTENCE:direct assay:GO:0004634 phosphopyruvate hydratase activity [PMID:23359425]
EXISTENCE:genetic interaction:GO:0004634 phosphopyruvate hydratase activity [PMID:23359425]
Enolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant
glutathione-independent methylglyoxalase family protein
EXISTENCE:direct assay:GO:0000932 P-body [PMID:24706893]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:24706893|PMID:26777405]
EXISTENCE:genetic interaction:GO:0051596 methylglyoxal catabolic process [PMID:27984092]
EXISTENCE:mutant phenotype:GO:0031669 cellular response to nutrient levels [PMID:24706893]
Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:26055717]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:genetic interaction:GO:1903424 fluoride transmembrane transport [PMID:24173035|PMID:26055717]
EXISTENCE:genetic interaction:GO:1903425 fluoride transmembrane transporter activity [PMID:24173035]
EXISTENCE:mutant phenotype:GO:1903424 fluoride transmembrane transport [PMID:26055717]
Protein involved in fluoride export; nearly identical to FEX1, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins
hypothetical protein; gene expression regulated by copper levels
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11935221]
hypothetical protein; localized to the membranes; gene expression regulated by copper levels
EXISTENCE:genetic interaction:GO:0008863 formate dehydrogenase (NAD+) activity [PMID:11921099]
EXISTENCE:genetic interaction:GO:0042183 formate catabolic process [PMID:11921099|PMID:19564482]
NAD(+)-dependent formate dehydrogenase; may protect cells from exogenous formate; YPL275W and YPL276W comprise a continuous open reading frame in some S. cerevisiae strains but not in the genomic reference strain S288C
ARS1603; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
bifunctional polyamine/amino acid permease SAM3
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17218313]
EXISTENCE:direct assay:GO:0015489 putrescine transmembrane transporter activity [PMID:17218313]
EXISTENCE:direct assay:GO:0015606 spermidine transmembrane transporter activity [PMID:17218313]
EXISTENCE:direct assay:GO:0015847 putrescine transport [PMID:17218313]
EXISTENCE:direct assay:GO:0015848 spermidine transport [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0000095 S-adenosyl-L-methionine transmembrane transporter activity [PMID:10497160]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:10497160]
EXISTENCE:mutant phenotype:GO:0015489 putrescine transmembrane transporter activity [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015606 spermidine transmembrane transporter activity [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015805 S-adenosyl-L-methionine transport [PMID:10497160]
EXISTENCE:mutant phenotype:GO:0015847 putrescine transport [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015848 spermidine transport [PMID:17218313]
High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
S-adenosylmethionine-homocysteine S-methyltransferase SAM4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0008898 S-adenosylmethionine-homocysteine S-methyltransferase activity [PMID:11013242]
EXISTENCE:direct assay:GO:0019284 L-methionine salvage from S-adenosylmethionine [PMID:14189892]
EXISTENCE:direct assay:GO:0046498 S-adenosylhomocysteine metabolic process [PMID:14189892]
EXISTENCE:direct assay:GO:0050667 homocysteine metabolic process [PMID:14189892]
S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
hypothetical protein; gene expression induced in response to ketoconazole; YPL272C is not an essential gene
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:17082766]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:15294286|PMID:18722382]
EXISTENCE:direct assay:GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1) [PMID:15294286]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:8416924]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:8416924]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:8416924]
Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11251115]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:23509072]
EXISTENCE:direct assay:GO:0070301 cellular response to hydrogen peroxide [PMID:23509072]
Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:26906737]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26906737]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26906737]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:20534809]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:26906737]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:9442113]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9442113]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:24850890]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:9442113]
EXISTENCE:mutant phenotype:GO:0051293 establishment of spindle localization [PMID:22385961]
Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:10779349|PMID:12756538]
EXISTENCE:direct assay:GO:0004435 phosphatidylinositol phospholipase C activity [PMID:15485855|PMID:8395015]
EXISTENCE:direct assay:GO:0009395 phospholipid catabolic process [PMID:8395015]
EXISTENCE:genetic interaction:GO:0001402 signal transduction involved in filamentous growth [PMID:10514491]
EXISTENCE:mutant phenotype:GO:0004435 phosphatidylinositol phospholipase C activity [PMID:7753023|PMID:9784626]
EXISTENCE:mutant phenotype:GO:0009395 phospholipid catabolic process [PMID:7753023]
EXISTENCE:mutant phenotype:GO:0032958 inositol phosphate biosynthetic process [PMID:10390371]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19205744]
EXISTENCE:physical interaction:GO:0000776 kinetochore [PMID:10779349]
EXISTENCE:physical interaction:GO:0001402 signal transduction involved in filamentous growth [PMID:10514491]
Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18498748]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18498748]
EXISTENCE:direct assay:GO:0055105 ubiquitin-protein transferase inhibitor activity [PMID:18498748|PMID:18519589|PMID:18596038]
EXISTENCE:direct assay:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:18519589|PMID:18596038]
EXISTENCE:genetic interaction:GO:0055105 ubiquitin-protein transferase inhibitor activity [PMID:18498748]
EXISTENCE:genetic interaction:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:17030612|PMID:18519589|PMID:18596038]
EXISTENCE:mutant phenotype:GO:0055105 ubiquitin-protein transferase inhibitor activity [PMID:18498748|PMID:18596038]
EXISTENCE:mutant phenotype:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:18596038|PMID:18519589]
EXISTENCE:physical interaction:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:18519589|PMID:18596038]
Pseudosubstrate inhibitor of the APC/C; suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein; the anaphase-promoting complex/cyclosome is also known as APC/C
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:15167894]
EXISTENCE:mutant phenotype:GO:0000154 rRNA modification [PMID:8064863]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8064863]
Essential 18S rRNA dimethylase (dimethyladenosine transferase); responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing; human homolog DIMT1 complements yeast dim1 mutant
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005310 dicarboxylic acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0006865 amino acid transport [PMID:10654085]
Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
ARS1604; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:31945465]
Endoplasmic reticulum hypothetical protein; physically interacts with Hsp82p; YPL264C is not an essential gene
EXISTENCE:direct assay:GO:0004333 fumarate hydratase activity [PMID:3040736]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20231875]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11585823]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11585823]
EXISTENCE:mutant phenotype:GO:0006099 tricarboxylic acid cycle [PMID:1587456]
EXISTENCE:mutant phenotype:GO:0006106 fumarate metabolic process [PMID:1587456]
Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
hypothetical protein; conserved among S. cerevisiae strains; YPL261C is not an essential gene; partially overlaps verified ORF YPL260W
Conserved fungal gene linked to DNA repair and proteasome function; putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0030121 AP-1 adaptor complex [PMID:10564262]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:17003107]
Mu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
EXISTENCE:direct assay:GO:0008902 hydroxymethylpyrimidine kinase activity [PMID:15614489]
EXISTENCE:direct assay:GO:0008972 phosphomethylpyrimidine kinase activity [PMID:15614489]
EXISTENCE:genetic interaction:GO:0008902 hydroxymethylpyrimidine kinase activity [PMID:15614489]
EXISTENCE:genetic interaction:GO:0008972 phosphomethylpyrimidine kinase activity [PMID:10383756|PMID:15614489]
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:10383756]
Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis
YPLWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
hypothetical protein; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7862657|PMID:8387915|PMID:10409718]
EXISTENCE:direct assay:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:2142620]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10611233|PMID:11080155|PMID:11792824]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:10409718|PMID:7862657|PMID:8387915]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:9927449]
EXISTENCE:genetic interaction:GO:0007089 traversing start control point of mitotic cell cycle [PMID:2574633|PMID:2147225]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:11509671]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:2569741]
G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:20139001]
EXISTENCE:direct assay:GO:0005823 central plaque of spindle pole body [PMID:10654940]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:10654940]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:10654940]
EXISTENCE:mutant phenotype:GO:0071988 protein localization to spindle pole body [PMID:14767068|PMID:11952896]
Protein required for the spindle pole body (SPB) duplication; localizes at the cytoplasmic side of the central plaque periphery of the SPB; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0005671 Ada2/Gcn5/Ada3 transcription activator complex [PMID:9154821]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:mutant phenotype:GO:0003712 transcription coregulator activity [PMID:9154821]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:10048020]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:9154821]
Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10087265|PMID:11729143]
EXISTENCE:direct assay:GO:0005871 kinesin complex [PMID:10087265]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:22734002]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714]
EXISTENCE:mutant phenotype:GO:0007017 microtubule-based process [PMID:10087265]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:33752231]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714]
EXISTENCE:physical interaction:GO:0007017 microtubule-based process [PMID:10087265]
Protein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication
ARS1605; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10375636]
EXISTENCE:genetic interaction:GO:0006784 heme A biosynthetic process [PMID:11788607]
EXISTENCE:genetic interaction:GO:0016653 oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor [PMID:11788607]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:21944752|PMID:20534343]
EXISTENCE:mutant phenotype:GO:0006784 heme A biosynthetic process [PMID:11788607]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:12970193]
EXISTENCE:mutant phenotype:GO:0016653 oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor [PMID:11788607]
Ferredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; human homolog FDX1L can complement yeast by allowing growth during down-regulation of yeast YAH1
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:26565778]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:26565778]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:26565778]
hypothetical protein; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15331637]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005798 Golgi-associated vesicle [PMID:15331637]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12189143|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15331637]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:15331637|PMID:22842922|PMID:32015451]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15331637|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:35134079]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:12189143|PMID:15802519]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:15802519]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:12189143]
GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication
Antisense to GAL4/YPL248C; influences expression of GAL4 mRNA
galactose-responsive transcription factor GAL4
EXISTENCE:direct assay:GO:0000435 positive regulation of transcription from RNA polymerase II promoter by galactose [PMID:3886158]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:3886158]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:3080805]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:6091123]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:14871930]
EXISTENCE:genetic interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:14871930]
EXISTENCE:mutant phenotype:GO:0000435 positive regulation of transcription from RNA polymerase II promoter by galactose [PMID:6350827]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:14871930]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:3080805]
EXISTENCE:mutant phenotype:GO:0006012 galactose metabolic process [PMID:6350827]
EXISTENCE:physical interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:14871930]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:14871930]
DNA-binding transcription factor required for activating GAL genes; responds to galactose; repressed by Gal80p and activated by Gal3p
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
Possible rhomboid protease; has similarity to eukaryotic rhomboid proteases including Pcp1p
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
EXISTENCE:genetic interaction:GO:0015786 UDP-glucose transmembrane transport [PMID:18693752]
EXISTENCE:mutant phenotype:GO:0015786 UDP-glucose transmembrane transport [PMID:18693752]
EXISTENCE:mutant phenotype:GO:0072334 UDP-galactose transmembrane transport [PMID:11284009]
Protein with a role in UDP-galactose transport to the Golgi lumen; has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
signal recognition particle subunit SRP68
EXISTENCE:direct assay:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:7925282]
EXISTENCE:direct assay:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:10921896]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:9382845|PMID:9442111|PMID:9950677|PMID:10873803]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11082046|PMID:22842922]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:9382845|PMID:9950677]
EXISTENCE:direct assay:GO:0120155 MIH complex [PMID:24895401]
EXISTENCE:mutant phenotype:GO:0051015 actin filament binding [PMID:9382845|PMID:9950677]
EXISTENCE:mutant phenotype:GO:0072741 protein localization to cell division site [PMID:21173112]
EXISTENCE:mutant phenotype:GO:1903471 regulation of mitotic actomyosin contractile ring contraction [PMID:9950677|PMID:19109423|PMID:26085509|PMID:24895401]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:11082046|PMID:21173112]
EXISTENCE:mutant phenotype:GO:1903479 mitotic actomyosin contractile ring assembly actin filament organization [PMID:9382845|PMID:9442111|PMID:9950677]
EXISTENCE:physical interaction:GO:0005516 calmodulin binding [PMID:9950677]
EXISTENCE:physical interaction:GO:0032027 myosin light chain binding [PMID:10873803]
EXISTENCE:physical interaction:GO:0032033 myosin II light chain binding [PMID:11082046]
EXISTENCE:physical interaction:GO:0032038 myosin II heavy chain binding [PMID:11082046]
EXISTENCE:physical interaction:GO:0120155 MIH complex [PMID:24895401]
Actin filament binding protein that enhances actin ring formation; IQGAP family member required for assembly and contraction of the AMR, prior to cytokinesis; localizes to the contractile ring during anaphase in an Mlc1p-dependent manner, recruiting Myo1p and Hof1p to the site, and promoting cytokinetic core complex assembly; specifies bud-site selection, localizing axial markers Bud4p and Cdc12p; regulated by Cdc28p; relocalizes from the bud neck to the cytoplasm upon replication stress
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:18376416]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:2407611]
EXISTENCE:genetic interaction:GO:0007021 tubulin complex assembly [PMID:9215891]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9215891]
GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:32920053]
EXISTENCE:direct assay:GO:0006458 'de novo' protein folding [PMID:10564510]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12235160]
EXISTENCE:direct assay:GO:0032212 positive regulation of telomere maintenance via telomerase [PMID:17954556]
EXISTENCE:direct assay:GO:0043248 proteasome assembly [PMID:12853471]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10564510]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:18268103]
EXISTENCE:mutant phenotype:GO:0006458 'de novo' protein folding [PMID:9371781]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:16487343]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:27068472]
EXISTENCE:mutant phenotype:GO:0032212 positive regulation of telomere maintenance via telomerase [PMID:17954556]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:9371781]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:12853471]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:27068472]
EXISTENCE:physical interaction:GO:0006626 protein targeting to mitochondrion [PMID:12526792]
Hsp90 chaperone; functionally redundant with Hsc82p; required for pheromone signaling; negative regulator of the Hsf1p-dependent heat shock response with Cpr7p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; promotes solubility of chaperone-substrate complexes; DNA damage induced nuclear import is Aha1p dependent; protein abundance increases in response to DNA replication stress; human homolog, HSP90AB1, complements null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22570489]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:26112308]
EXISTENCE:genetic interaction:GO:0000056 ribosomal small subunit export from nucleus [PMID:22570489]
EXISTENCE:genetic interaction:GO:0005634 nucleus [PMID:22570489]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:22570489]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:22570489]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:22570489]
EXISTENCE:physical interaction:GO:0032880 regulation of protein localization [PMID:22570489]
Nucleocytoplasmic shuttling assembly chaperone; co-translationally associates with nascent Rps3p in the cytoplasm and delivers it to pre-ribosomal subunits in the nucleus; required for 40S ribosomal subunit nuclear export, biogenesis and adaptation to osmotic and oxidative stress; contains ankyrin-repeats; expression repressed by heat shock
translation initiation factor eIF2 subunit beta
EXISTENCE:direct assay:GO:0001731 formation of translation preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:9858542]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14698289|PMID:16246727]
EXISTENCE:direct assay:GO:0005850 eukaryotic translation initiation factor 2 complex [PMID:12137565]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:15145951|PMID:9395514|PMID:22683627]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:15838098|PMID:11018020]
EXISTENCE:direct assay:GO:1990856 methionyl-initiator methionine tRNA binding [PMID:16565414]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:16449648]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:12137565]
EXISTENCE:mutant phenotype:GO:0005850 eukaryotic translation initiation factor 2 complex [PMID:12137565]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:9395514|PMID:15145951|PMID:22683627]
Beta subunit of the translation initiation factor eIF2; involved in the identification of the start codon; proposed to be involved in mRNA binding
putative serine/threonine protein kinase ENV7
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:21912603]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:24610781]
Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; targeted to vacuole via AP-3 pathway; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
RuvB family ATP-dependent DNA helicase reptin
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:16299513]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11604509]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:10952318|PMID:20553504]
EXISTENCE:direct assay:GO:0043139 5'-3' DNA helicase activity [PMID:18234224]
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
EXISTENCE:direct assay:GO:0097255 R2TP complex [PMID:15766533]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:11604509|PMID:18268103]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11278922]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11604509]
EXISTENCE:physical interaction:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:14690608]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:10952318|PMID:24034245]
EXISTENCE:physical interaction:GO:0097255 R2TP complex [PMID:18268103]
ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
H(+)-transporting V0 sector ATPase subunit c'
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11853670]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15252052]
Vacuolar ATPase V0 domain subunit c'; involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
EXISTENCE:direct assay:GO:0000444 MIS12/MIND type complex [PMID:14633972]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14657030]
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:12455957]
EXISTENCE:direct assay:GO:0007059 chromosome segregation [PMID:12455957]
Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19502581]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:16339720]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:19502581]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:17298976|PMID:19502581]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17298976|PMID:19502581|PMID:16622836]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9195974]
EXISTENCE:direct assay:GO:0043325 phosphatidylinositol-3,4-bisphosphate binding [PMID:19502581]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:19502581]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:19502581]
EXISTENCE:genetic interaction:GO:0031321 ascospore-type prospore assembly [PMID:19502581]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11839791]
EXISTENCE:mutant phenotype:GO:0031321 ascospore-type prospore assembly [PMID:16554438|PMID:19502581]
EXISTENCE:physical interaction:GO:0005484 SNAP receptor activity [PMID:7954793|PMID:10924463]
Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication
trifunctional fatty acid synthase subunit FAS2
EXISTENCE:direct assay:GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity [PMID:365179]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:6025308|PMID:26928762]
EXISTENCE:direct assay:GO:0005835 fatty acid synthase complex [PMID:65153]
EXISTENCE:mutant phenotype:GO:0004312 fatty acid synthase activity [PMID:65153]
EXISTENCE:mutant phenotype:GO:0004315 3-oxoacyl-[acyl-carrier-protein] synthase activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0008897 holo-[acyl-carrier-protein] synthase activity [PMID:10785388]
EXISTENCE:mutant phenotype:GO:0042759 long-chain fatty acid biosynthetic process [PMID:1735446]
Alpha subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18667581|PMID:27663510]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363|PMID:19111667]
EXISTENCE:mutant phenotype:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:18667581]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:18667581]
Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication
ARS1607; Autonomously Replicating Sequence
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004651 polynucleotide 5'-phosphatase activity [PMID:9345280|PMID:17707331]
EXISTENCE:direct assay:GO:0098501 polynucleotide dephosphorylation [PMID:9345280]
EXISTENCE:genetic interaction:GO:0031533 mRNA cap methyltransferase complex [PMID:9710603]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24172134]
EXISTENCE:mutant phenotype:GO:1900182 positive regulation of protein localization to nucleus [PMID:24205062]
EXISTENCE:physical interaction:GO:0006370 7-methylguanosine mRNA capping [PMID:9345280]
EXISTENCE:physical interaction:GO:0031533 mRNA cap methyltransferase complex [PMID:9710603]
RNA 5'-triphosphatase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of a Cet1p homodimer and two molecules of guanylyltransferase Ceg1p; Cet1p also has a role in regulation of RNAPII pausing at promoter-proximal sites; interaction between Cet1p and Ceg1p is required for Ceg1p nuclear import; mammalian enzyme is single bifunctional polypeptide; human homolog RNGTT can complement yeast cet1 null mutant
dolichyl-phosphate beta-glucosyltransferase
EXISTENCE:direct assay:GO:0004581 dolichyl-phosphate beta-glucosyltransferase activity [PMID:8076653]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9560251]
UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:19806183]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:31299085]
EXISTENCE:mutant phenotype:GO:0006449 regulation of translational termination [PMID:31299085]
EXISTENCE:mutant phenotype:GO:0008079 translation termination factor activity [PMID:31299085]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:19806183]
Translation termination and ribosome biogenesis factor; ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
hypothetical protein; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9353309|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:9353309]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9353309]
Putative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0018117 protein adenylylation [PMID:30270044]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:30270044]
EXISTENCE:direct assay:GO:0070733 protein adenylyltransferase activity [PMID:30270044]
Mitochondrial AMPylator in the pseudokinase selenoprotein-O family; role in the oxidative stress response, transferring AMP from ATP to protein substrates (AMPylation) involved in redox homeostasis; regulates S-glutathionylation levels; proposed involvement in the environmental stress response
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16717099]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095]
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:27462707]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0015230 FAD transmembrane transporter activity [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0015883 FAD transport [PMID:16717099]
Flavin adenine dinucleotide transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:31439809]
Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal; ribosomes containing Rpl1b are more efficient in translation of respiration-related proteins
EXISTENCE:genetic interaction:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:9584169]
EXISTENCE:mutant phenotype:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:9584169]
Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003400 regulation of COPII vesicle coating [PMID:14627716|PMID:15665868]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8419365|PMID:9756629]
EXISTENCE:direct assay:GO:0016050 vesicle organization [PMID:16122427]
EXISTENCE:direct assay:GO:0030127 COPII vesicle coat [PMID:8004676]
EXISTENCE:direct assay:GO:0044233 mitochondria-associated endoplasmic reticulum membrane [PMID:27101143]
EXISTENCE:direct assay:GO:0070971 endoplasmic reticulum exit site [PMID:22675024]
EXISTENCE:genetic interaction:GO:0000266 mitochondrial fission [PMID:27101143]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:31406088]
EXISTENCE:mutant phenotype:GO:0007006 mitochondrial membrane organization [PMID:27101143]
EXISTENCE:mutant phenotype:GO:0061024 membrane organization [PMID:27101143]
ARF family GTPase; component of the COPII vesicle coat; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity aiding vesicle formation; localizes to ER-mitochondrial contact sites where it enhances membrane curvature, thereby reducing contact size via its N-terminal amphipathic helix; regulates mitochondrial fission and fusion dynamics
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16307926]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:16307926]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11565749|PMID:10684247]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:16307926|PMID:12150911]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:16307926]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11565749]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11565749]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:16307926]
EXISTENCE:mutant phenotype:GO:0005525 GTP binding [PMID:11565749|PMID:16307926]
GTPase required for ribosomal subunit synthesis and rRNA processing; required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
hypothetical protein; not an essential gene; YPL216W has a paralog, ITC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23509072]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:29475949]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11522252|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005761 mitochondrial ribosome [PMID:21670217]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:11522252]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:21670217]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:11522252|PMID:15759116]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:21670217]
EXISTENCE:physical interaction:GO:0043022 ribosome binding [PMID:21670217]
EXISTENCE:physical interaction:GO:0061671 Cbp3p-Cbp6 complex [PMID:21670217]
Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16850348]
EXISTENCE:mutant phenotype:GO:0004417 hydroxyethylthiazole kinase activity [PMID:7982968]
EXISTENCE:mutant phenotype:GO:0004789 thiamine-phosphate diphosphorylase activity [PMID:7982968]
EXISTENCE:mutant phenotype:GO:0009228 thiamine biosynthetic process [PMID:7982968]
Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase; required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:9799242]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9799242]
EXISTENCE:physical interaction:GO:0005686 U2 snRNP [PMID:9799242]
Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8641292]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:10356324]
EXISTENCE:direct assay:GO:0031119 tRNA pseudouridine synthesis [PMID:10356324]
EXISTENCE:mutant phenotype:GO:0031120 snRNA pseudouridine synthesis [PMID:10022901]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25192136|PMID:25219674]
tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9271378|PMID:14562095]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9271378]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9271378]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:9271378]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
Nucleolar protein required for 60S ribosome subunit biogenesis; constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
signal recognition particle subunit SRP72
Core component of the signal recognition particle (SRP); the SRP is a ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11511347|PMID:12566427]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:10072382|PMID:23142046|PMID:25387524|PMID:16319894|P MID:16143104]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:12566427]
EXISTENCE:direct assay:GO:0005828 kinetochore microtubule [PMID:11724818]
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:22842922]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11511347|PMID:11724818]
EXISTENCE:direct assay:GO:0032133 chromosome passenger complex [PMID:19158380]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:12566427]
EXISTENCE:genetic interaction:GO:0044774 mitotic DNA integrity checkpoint signaling [PMID:16651657]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8293973]
EXISTENCE:mutant phenotype:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:10072382]
EXISTENCE:mutant phenotype:GO:0032465 regulation of cytokinesis [PMID:16615892]
EXISTENCE:mutant phenotype:GO:0045143 homologous chromosome segregation [PMID:17289568]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:17289568|PMID:17371833]
EXISTENCE:mutant phenotype:GO:0051228 mitotic spindle disassembly [PMID:12566427]
EXISTENCE:mutant phenotype:GO:0090266 regulation of mitotic cell cycle spindle assembly checkpoint [PMID:16327780]
EXISTENCE:mutant phenotype:GO:1901673 regulation of mitotic spindle assembly [PMID:24386320]
EXISTENCE:mutant phenotype:GO:1901925 negative regulation of protein import into nucleus during spindle assembly checkpoint [PMID:23177738]
Aurora kinase of chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in meiosis I, and kinetochore release from SPB clusters at meiotic exit; helps maintain condensed chromosomes during anaphase; required for SPB cohesion and prevention of multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:16096273]
EXISTENCE:direct assay:GO:0018027 peptidyl-lysine dimethylation [PMID:16096273]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:16096273]
EXISTENCE:mutant phenotype:GO:0018026 peptidyl-lysine monomethylation [PMID:22522802]
EXISTENCE:mutant phenotype:GO:0018027 peptidyl-lysine dimethylation [PMID:16096273]
SET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp)
putative tRNA 4-demethylwyosine synthase
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0031591 wybutosine biosynthetic process [PMID:34184886]
EXISTENCE:direct assay:GO:1904047 S-adenosyl-L-methionine binding [PMID:34184886]
EXISTENCE:mutant phenotype:GO:0031591 wybutosine biosynthetic process [PMID:16162496|PMID:16642040|PMID:17150819]
Iron-sulfur protein required for synthesis of wybutosine modified tRNA; wybutosine is a modified guanosine found at 3'-position adjacent to anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
ARS1608; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:18434318|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:12514182|PMID:24868093]
EXISTENCE:direct assay:GO:0034479 phosphatidylglycerol phospholipase C activity [PMID:18434318]
EXISTENCE:mutant phenotype:GO:0034478 phosphatidylglycerol catabolic process [PMID:18434318]
EXISTENCE:mutant phenotype:GO:0034479 phosphatidylglycerol phospholipase C activity [PMID:18434318]
EXISTENCE:mutant phenotype:GO:0046475 glycerophospholipid catabolic process [PMID:16172116]
Phosphatidylglycerol phospholipase C; regulates phosphatidylglycerol (PG) accumulation via a phospholipase C-type degradation mechanism; PG levels affect mitochondrial function; contains glycerophosphodiester phosphodiesterase motifs
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:26195667]
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:16990132]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:29915153]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:1495994|PMID:21532587|PMID:25569479]
EXISTENCE:direct assay:GO:0004713 protein tyrosine kinase activity [PMID:7803855]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8034689]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:22375831]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:21532587]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:17461799]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8034689]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:14597664|PMID:17461799]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14597664|PMID:17461799|PMID:26928762]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:32317286]
EXISTENCE:direct assay:GO:0033551 monopolin complex [PMID:16990132]
EXISTENCE:direct assay:GO:0042274 ribosomal small subunit biogenesis [PMID:25778921]
EXISTENCE:direct assay:GO:0060628 regulation of ER to Golgi vesicle-mediated transport [PMID:21532587]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:16990132]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:1887218]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:25287303]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:14597664]
EXISTENCE:mutant phenotype:GO:0033551 monopolin complex [PMID:16990132]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:18256024]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:16738661|PMID:25778921]
EXISTENCE:mutant phenotype:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:16990132]
EXISTENCE:mutant phenotype:GO:0060628 regulation of ER to Golgi vesicle-mediated transport [PMID:21532587]
EXISTENCE:mutant phenotype:GO:0106214 regulation of vesicle fusion with Golgi apparatus [PMID:26195667]
EXISTENCE:mutant phenotype:GO:2000370 positive regulation of clathrin-dependent endocytosis [PMID:25287303]
EXISTENCE:mutant phenotype:GO:2000785 regulation of autophagosome assembly [PMID:26195667]
EXISTENCE:mutant phenotype:GO:2001159 regulation of protein localization by the Cvt pathway [PMID:24968893|PMID:25287303]
EXISTENCE:physical interaction:GO:0033551 monopolin complex [PMID:16990132]
Conserved casein kinase, monopolin subunit; regulates diverse events including vesicular traffic, DNA repair, CVT pathway, cohesion cleavage, attachment of sister kinetochores at meiosis I, exit from meiosis II, and ribosomal subunit biogenesis; binds the RNAPII CTD; phosphorylates COPII coat subunits; interacts with Sit4p phosphatase; antagonizes calcineurin signaling, reducing nuclear accumulation of Crz1p; phosphorylates Dsn1p; homolog of mammalian CK1delta
cAMP-dependent protein kinase catalytic subunit TPK2
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:26403272]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:22899713]
EXISTENCE:direct assay:GO:0004691 cAMP-dependent protein kinase activity [PMID:12024012|PMID:36167807]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12024012|PMID:18417610|PMID:22932476]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0005952 cAMP-dependent protein kinase complex [PMID:12024012]
EXISTENCE:genetic interaction:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:genetic interaction:GO:0005952 cAMP-dependent protein kinase complex [PMID:3036373]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:3036373]
EXISTENCE:genetic interaction:GO:0010607 negative regulation of cytoplasmic mRNA processing body assembly [PMID:21925385]
EXISTENCE:genetic interaction:GO:0010737 protein kinase A signaling [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0004691 cAMP-dependent protein kinase activity [PMID:36167807]
EXISTENCE:mutant phenotype:GO:0071333 cellular response to glucose stimulus [PMID:36167807]
EXISTENCE:mutant phenotype:GO:2000766 negative regulation of cytoplasmic translation [PMID:22899713]
EXISTENCE:physical interaction:GO:0005952 cAMP-dependent protein kinase complex [PMID:12024012]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:22899713]
cAMP-dependent protein kinase catalytic subunit; promotes nutrient responsive growth via the Ras-cAMP signaling pathway; phosphorylates and inhibits histone demethylase Jhd2p in response to glucose, promoting H3K4 trimethylation and maintaining H3K14 acetylation by preventing chromatin binding of Rpd3p; required for Sfl1p-mediated pseudohyphal growth; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:16024809]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:27402848]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11448968]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:16024809]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:11448968]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16024809]
EXISTENCE:mutant phenotype:GO:0032048 cardiolipin metabolic process [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0051536 iron-sulfur cluster binding [PMID:31493153]
EXISTENCE:mutant phenotype:GO:2000185 regulation of phosphate transmembrane transport [PMID:27618952]
Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16358322]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16358322]
EXISTENCE:mutant phenotype:GO:0006114 glycerol biosynthetic process [PMID:16358322]
Protein that interacts with glycerol 3-phosphatase; plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:18585352]
EXISTENCE:direct assay:GO:0005640 nuclear outer membrane [PMID:32059771]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12514182]
EXISTENCE:genetic interaction:GO:0045141 meiotic telomere clustering [PMID:18585352]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:21339478]
EXISTENCE:mutant phenotype:GO:0010520 regulation of reciprocal meiotic recombination [PMID:18818741|PMID:18818742]
EXISTENCE:mutant phenotype:GO:0034993 meiotic nuclear membrane microtubule tethering complex [PMID:32059771]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0045141 meiotic telomere clustering [PMID:18818742|PMID:18585352]
EXISTENCE:mutant phenotype:GO:0140444 cytoskeleton-nuclear membrane anchor activity [PMID:32059771]
Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements; functions with meiosis-specific telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:32234470]
Protein involved in nonstop mRNA decay; predicted to be palmitoylated
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:23874617]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:23874617]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19466602]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19466602]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:23874617]
EXISTENCE:direct assay:GO:0042273 ribosomal large subunit biogenesis [PMID:23945946]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22893726]
Ribosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A807
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:34918374]
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:34918374]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15060142]
EXISTENCE:direct assay:GO:0007035 vacuolar acidification [PMID:34918374]
Protein involved in regulation of vacuolar V-ATPase; inhibits V1-ATPase activity and triggers disassembly of V-ATPase holoenzyme; required for normal levels of resistance to oxidative damage; null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17895371]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:9335339]
EXISTENCE:mutant phenotype:GO:0030123 AP-3 adaptor complex [PMID:9250663]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10559961]
Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3
EXISTENCE:direct assay:GO:0000725 recombinational repair [PMID:16840526]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:11825877]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:31340146]
EXISTENCE:direct assay:GO:0030896 checkpoint clamp complex [PMID:12604797|PMID:9670034|PMID:9891048]
EXISTENCE:direct assay:GO:0032147 activation of protein kinase activity [PMID:31340146]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:16840526]
EXISTENCE:mutant phenotype:GO:0007095 mitotic G2 DNA damage checkpoint signaling [PMID:9311982]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:9311982]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:9311982]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:11825877]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:11825877]
DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:10567587]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:10567587]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:25170085]
Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12514182]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:19297527]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12514182|PMID:19297527]
EXISTENCE:mutant phenotype:GO:0005635 nuclear envelope [PMID:12514182]
EXISTENCE:mutant phenotype:GO:0048288 nuclear membrane fusion involved in karyogamy [PMID:19297527]
Protein required for nuclear envelope fusion during karyogamy; pheromone-regulated; peripheral protein of the nuclear membrane; interacts with Kar5p at the spindle pole body
EXISTENCE:direct assay:GO:0071108 protein K48-linked deubiquitination [PMID:27292798]
EXISTENCE:direct assay:GO:1990380 K48-linked deubiquitinase activity [PMID:27292798]
K48-specific deubiquitinating (DUB) enzyme; MINDY family endo-type deubiquitinase that preferentially cleaves long K48-linked polyubiquitin chains between moieties; diploid deletion strain exhibits high budding index; GFP-fusion protein localizes to the cytoplasm endoplasmic reticulum and cell periphery in high-throughput studies; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication; ortholog of human MINDY2/FAM63B
EXISTENCE:direct assay:GO:0001068 transcription regulatory region RNA binding [PMID:21084293]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:20805243|PMID:31960028]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7962083]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:16427013]
EXISTENCE:direct assay:GO:0035649 Nrd1 complex [PMID:10655211]
EXISTENCE:direct assay:GO:0071041 antisense RNA transcript catabolic process [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0034472 snRNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0042780 tRNA 3'-end processing [PMID:21460797]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:21113025]
EXISTENCE:mutant phenotype:GO:0071034 CUT catabolic process [PMID:16973436]
EXISTENCE:mutant phenotype:GO:0071041 antisense RNA transcript catabolic process [PMID:22028667]
RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:18541668]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:18541668]
EXISTENCE:physical interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:18541668]
Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
EXISTENCE:genetic interaction:GO:0015793 glycerol transmembrane transport [PMID:10931309]
Probable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; homolog of the mammalian Hedgehog pathway modulator HHAT; GUP2 has a paralog, GUP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:12727869]
EXISTENCE:direct assay:GO:0006741 NADP biosynthetic process [PMID:12727869]
EXISTENCE:direct assay:GO:0042736 NADH kinase activity [PMID:12727869]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:20889970]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:7586028]
Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
EXISTENCE:direct assay:GO:0000772 mating pheromone activity [PMID:6306574]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:776628]
EXISTENCE:direct assay:GO:1903135 cupric ion binding [PMID:27476515]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3887136]
EXISTENCE:mutant phenotype:GO:0000772 mating pheromone activity [PMID:3887136]
Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor; binds copper(II) ions
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11056382]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11056382|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:35171083]
Protein required for nuclear envelope integrity; involved in distribution of nuclear pore complexes; interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23028530]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23028530|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:23028530]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:23028530]
RNA-binding protein involved in translational regulation; links cell wall homeostasis to mitochondrial structure and function; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
putative mitochondrial 54S ribosomal protein RTC6
EXISTENCE:genetic interaction:GO:0042254 ribosome biogenesis [PMID:20691087]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:20691087]
Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21880895|PMID:14562095]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:21880895]
EXISTENCE:mutant phenotype:GO:0002130 wobble position ribose methylation [PMID:22912484]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:21880895]
EXISTENCE:physical interaction:GO:0002130 wobble position ribose methylation [PMID:22912484]
WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:17142463]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:19417103]
EXISTENCE:mutant phenotype:GO:0045893 positive regulation of DNA-templated transcription [PMID:12086626]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:16286008]
Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
EXISTENCE:curator inference:GO:0031929 TOR signaling [PMID:14736892]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:17560372|PMID:19748353|PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006071 glycerol metabolic process [PMID:10931309]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:17298473]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:14736892]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:14736892]
EXISTENCE:physical interaction:GO:0031931 TORC1 complex [PMID:14736892]
Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:24309106]
EXISTENCE:mutant phenotype:GO:0090029 negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:24309106]
Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin
nuclear cap-binding protein subunit CBC2
EXISTENCE:direct assay:GO:0000243 commitment complex [PMID:8682299]
EXISTENCE:direct assay:GO:0000339 RNA cap binding [PMID:8682299]
EXISTENCE:direct assay:GO:0005846 nuclear cap binding complex [PMID:8811086]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:8682299]
Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
EXISTENCE:curator inference:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:18708352|PMID:9427760]
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9427760]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9427760]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10850718|PMID:17005992|PMID:18708352|PMID:9427760]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:18708352]
Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16456538|PMID:26928762]
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:19369420]
Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitination and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
phosphatidylinositol N-acetylglucosaminyltransferase SPT14
EXISTENCE:curator inference:GO:0006506 GPI anchor biosynthetic process [PMID:10970797]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:9079905]
EXISTENCE:mutant phenotype:GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity [PMID:10970797]
UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
ARS1611; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:9658168]
EXISTENCE:direct assay:GO:0005869 dynactin complex [PMID:9658168]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:9658168]
Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
mitochondrial 54S ribosomal protein YmL40
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:mutant phenotype:GO:0006784 heme A biosynthetic process [PMID:8118433]
Heme A:farnesyltransferase; catalyzes first step in conversion of protoheme to heme A prosthetic group required for cytochrome c oxidase activity; human ortholog COX10 can complement yeast cox10 null mutant; human ortholog COX10 is associated with mitochondrial disorders
EXISTENCE:direct assay:GO:0003959 NADPH dehydrogenase activity [PMID:7836424]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17897954]
Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17954932]
EXISTENCE:direct assay:GO:0020037 heme binding [PMID:15713626]
EXISTENCE:genetic interaction:GO:0032443 regulation of ergosterol biosynthetic process [PMID:15713626]
EXISTENCE:mutant phenotype:GO:0032443 regulation of ergosterol biosynthetic process [PMID:17954932]
Heme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:29212662]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:12524544]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:31753862]
EXISTENCE:direct assay:GO:0015288 porin activity [PMID:31753862]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030619 U1 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030621 U4 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030623 U5 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:31753862]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:17434126]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:17434126|PMID:31439809]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:22956913]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:10722667]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:29212662]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:29212662]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0051168 nuclear export [PMID:31189105]
EXISTENCE:mutant phenotype:GO:0071528 tRNA re-export from nucleus [PMID:33074312]
EXISTENCE:physical interaction:GO:0042272 nuclear RNA export factor complex [PMID:9774696]
Poly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore and nuclear RNA export factor; ubiquitin-associated (UBA) domain interacts with Mip6p; ortholog of human TAP
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:8658138]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16452144]
EXISTENCE:direct assay:GO:0016035 zeta DNA polymerase complex [PMID:8658138|PMID:22711820|PMID:23066099]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:11313481|PMID:17248528]
EXISTENCE:direct assay:GO:0070987 error-free translesion synthesis [PMID:12514101]
EXISTENCE:genetic interaction:GO:0042276 error-prone translesion synthesis [PMID:9765213]
Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; may be involved in meiosis; forms a complex with Rev7p, Pol31p and Pol32p
EXISTENCE:direct assay:GO:0000045 autophagosome assembly [PMID:18725539]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:26774783]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:16289106]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0034497 protein localization to phagophore assembly site [PMID:26774783]
EXISTENCE:direct assay:GO:1990316 Atg1/ULK1 kinase complex [PMID:26753620]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:16289106]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
SET domain hypothetical protein; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:23316435]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:10679328|PMID:9770499]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10570173]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:10570173]
EXISTENCE:physical interaction:GO:0097587 MutLgamma complex [PMID:9770499|PMID:10570173]
Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11935221]
Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
EXISTENCE:mutant phenotype:GO:0007266 Rho protein signal transduction [PMID:8754839]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8754839]
Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
EXISTENCE:direct assay:GO:0004823 leucine-tRNA ligase activity [PMID:9742237]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9742237]
EXISTENCE:direct assay:GO:0006429 leucyl-tRNA aminoacylation [PMID:9742237]
EXISTENCE:mutant phenotype:GO:1903432 regulation of TORC1 signaling [PMID:22424774]
EXISTENCE:physical interaction:GO:1903432 regulation of TORC1 signaling [PMID:22424774]
Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature
EXISTENCE:direct assay:GO:0000262 mitochondrial chromosome [PMID:31532710]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16491469]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:16491469]
Mitochondrial protein; required for respiratory growth under some conditions and for stability of the mitochondrial genome; subunit of a complex containing Mrx6p, Pim1p, and Mam33p that may regulate mtDNA replication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:28148650]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:25416945]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:30341817]
EXISTENCE:direct assay:GO:0032177 cellular bud neck split septin rings [PMID:30341817]
EXISTENCE:genetic interaction:GO:1990344 secondary cell septum biogenesis [PMID:23468594]
EXISTENCE:mutant phenotype:GO:0005637 nuclear inner membrane [PMID:30341817]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:30341817]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:25416945]
EXISTENCE:mutant phenotype:GO:0045185 maintenance of protein location [PMID:23468594]
EXISTENCE:mutant phenotype:GO:0098841 protein localization to cell division site after cytokinesis [PMID:25416945]
EXISTENCE:mutant phenotype:GO:1901900 regulation of protein localization to cell division site [PMID:23468594]
EXISTENCE:mutant phenotype:GO:1903473 positive regulation of mitotic actomyosin contractile ring contraction [PMID:24451263]
EXISTENCE:mutant phenotype:GO:1990344 secondary cell septum biogenesis [PMID:23468594]
Regulator of Cdc42p and Rho1p; regulates AMR closure through Hof1p; inhibits Cdc42-dependent Cla4 activation at the division site, to prevent budding in the old bud neck; recruits Nis1p and Nba1p to the division site with Nap1 and the Rax1p-Rax2p dependent inheritance of Nis1p and Nba1p to bud scars to prevent division site repolarization; keeps Rho1p at the division site after AMR contraction to control secondary septum formation; relocalizes from bud neck to cytoplasm upon replication stress
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11983179]
EXISTENCE:mutant phenotype:GO:0001510 RNA methylation [PMID:11983179]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:27348091]
EXISTENCE:mutant phenotype:GO:0008173 RNA methyltransferase activity [PMID:11983179]
EXISTENCE:mutant phenotype:GO:0017126 nucleologenesis [PMID:15340060]
EXISTENCE:mutant phenotype:GO:0032210 regulation of telomere maintenance via telomerase [PMID:18273059|PMID:18840651]
EXISTENCE:mutant phenotype:GO:0036261 7-methylguanosine cap hypermethylation [PMID:11983179]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:21398639]
Trimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p
Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:31490122]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:9693366]
EXISTENCE:direct assay:GO:0007018 microtubule-based movement [PMID:31490122]
EXISTENCE:direct assay:GO:0007026 negative regulation of microtubule depolymerization [PMID:26576948]
EXISTENCE:direct assay:GO:0046785 microtubule polymerization [PMID:26576948]
EXISTENCE:direct assay:GO:1903754 cortical microtubule plus-end [PMID:15177030|PMID:18411245]
EXISTENCE:genetic interaction:GO:0008574 plus-end-directed microtubule motor activity [PMID:15177030|PMID:18411245]
EXISTENCE:mutant phenotype:GO:0007026 negative regulation of microtubule depolymerization [PMID:9693366]
EXISTENCE:mutant phenotype:GO:0008574 plus-end-directed microtubule motor activity [PMID:15177030]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:9693366]
Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0008233 peptidase activity [PMID:7002931]
EXISTENCE:mutant phenotype:GO:0000425 pexophagy [PMID:31332264]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:26947009]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:23382696]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:2674123]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:29514932]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:12529432]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0051603 proteolysis involved in protein catabolic process [PMID:16183335]
Vacuolar aspartyl protease (proteinase A); required for post-translational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
ARS1612; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
serine/threonine/tyrosine protein kinase RAD53
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16816422]
EXISTENCE:direct assay:GO:0004712 protein serine/threonine/tyrosine kinase activity [PMID:8355715|PMID:30377154]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8355715|PMID:22932476]
EXISTENCE:genetic interaction:GO:0000077 DNA damage checkpoint signaling [PMID:7926756]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16816422]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:8355715]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:18045534]
EXISTENCE:mutant phenotype:GO:0009202 deoxyribonucleoside triphosphate biosynthetic process [PMID:17188244]
EXISTENCE:mutant phenotype:GO:0042326 negative regulation of phosphorylation [PMID:24130507]
EXISTENCE:mutant phenotype:GO:1901857 positive regulation of cellular respiration [PMID:31073026]
DNA damage response kinase; signal transduction pathway component required for DNA damage and replication checkpoints, promoting cell cycle arrest and DNA repair; role in initiation of DNA replication; inhibits gene gating through NPC protein phosphorylation, to promote fork stability; activates downstream kinase Dun1p; senses mtDNA depletion and mitochondrial ROS; relocalizes to cytosol under hypoxia; contains two FHA domains; human homolog CHEK2 implicated in breast cancer complements the null
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0000159 protein phosphatase type 2A complex [PMID:17550305]
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:16380387]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:10660069]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:11262194]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:12952889]
Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:11884590]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:12554883]
EXISTENCE:physical interaction:GO:0000974 Prp19 complex [PMID:12088152]
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
non-specific serine/threonine protein kinase
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:direct assay:GO:0036464 cytoplasmic ribonucleoprotein granule [PMID:25342717]
Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:11689437|PMID:18497569]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005776 autophagosome [PMID:15155809]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11897782|PMID:26928762]
EXISTENCE:direct assay:GO:0006501 C-terminal protein lipidation [PMID:17986448]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:23503366]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:23064152]
EXISTENCE:direct assay:GO:0019776 Atg8 ligase activity [PMID:17986448]
EXISTENCE:direct assay:GO:0034274 Atg12-Atg5-Atg16 complex [PMID:11897782]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:direct assay:GO:0120095 vacuole-isolation membrane contact site [PMID:23549786]
EXISTENCE:direct assay:GO:0140355 cargo receptor ligand activity [PMID:27879200]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:8921905|PMID:32788210]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10712513]
EXISTENCE:mutant phenotype:GO:0061912 selective autophagy [PMID:27879200]
Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3 by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; also involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0008897 holo-[acyl-carrier-protein] synthase activity [PMID:9712852]
EXISTENCE:direct assay:GO:0031108 holo-[acyl-carrier-protein] biosynthetic process [PMID:9712852]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9712852]
Phosphopantetheine:protein transferase (PPTase); activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
ATP-binding cassette long-chain fatty acid transporter PXA1
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:8670886|PMID:35127709]
EXISTENCE:direct assay:GO:0015867 ATP transport [PMID:35127709]
EXISTENCE:direct assay:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:35127709]
EXISTENCE:mutant phenotype:GO:0015908 fatty acid transport [PMID:8670886]
EXISTENCE:mutant phenotype:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:8670886]
Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transporters ABCD1and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16043506|PMID:10684247|PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16043506|PMID:10684247]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:16128814]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:16043506]
EXISTENCE:mutant phenotype:GO:0000176 nuclear exosome (RNase complex) [PMID:31662437]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15686447]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:16128814]
Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p and with the nucleolar proteins Nop17p and Nip7p; null mutant is viable but growth is severely impaired
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:11916983]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8978672|PMID:14562095]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0010922 positive regulation of phosphatase activity [PMID:21295699]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:16585271|PMID:20008566]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:20729555]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:20729555]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:20008566]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006892 post-Golgi vesicle-mediated transport [PMID:11916983|PMID:8978672]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
EXISTENCE:mutant phenotype:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:11916983]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:23593226]
EXISTENCE:mutant phenotype:GO:0008142 oxysterol binding [PMID:16136145]
EXISTENCE:mutant phenotype:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:20008566]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:24213531]
EXISTENCE:mutant phenotype:GO:0120015 sterol transfer activity [PMID:20008566]
Sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger; one of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17707236]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:17707236]
EXISTENCE:genetic interaction:GO:0051131 chaperone-mediated protein complex assembly [PMID:17707236]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:18278055|PMID:18260085]
EXISTENCE:mutant phenotype:GO:0051131 chaperone-mediated protein complex assembly [PMID:17707236]
EXISTENCE:physical interaction:GO:0051131 chaperone-mediated protein complex assembly [PMID:18278055]
Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689|PMID:8455623]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:15231838|PMID:12150911]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:genetic interaction:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0003723 RNA binding [PMID:7556076]
EXISTENCE:genetic interaction:GO:0019843 rRNA binding [PMID:7556076]
EXISTENCE:mutant phenotype:GO:0019843 rRNA binding [PMID:7556076]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
Small nucleolar RNA (snoRNA) U3; part of small (ribosomal) subunit (SSU) processosome; SNR17A also encodes snoRNA U3
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:17548477]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:17548477]
Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:14562095]
Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
putative mitogen-activated protein kinase kinase MKK2
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12361575]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12361575]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12361575]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12361575]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:8386320]
EXISTENCE:genetic interaction:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:8386320]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:8386320]
MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
ARS1614; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9234739]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9234739|PMID:25398614]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25398614]
EXISTENCE:direct assay:GO:0032221 Rpd3S/Clr6-CII complex [PMID:16286007|PMID:16286008]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19270272]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:9234739]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17210643|PMID:20398213]
Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18845545]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:direct assay:GO:0051568 histone H3-K4 methylation [PMID:11742990|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0003682 chromatin binding [PMID:23246437]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11687631]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11687631|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11752412]
EXISTENCE:mutant phenotype:GO:0051568 histone H3-K4 methylation [PMID:11752412]
EXISTENCE:mutant phenotype:GO:1903341 regulation of meiotic DNA double-strand break formation [PMID:23246437]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; promotes meiotic DSB formation by interacting with H3K4me3 and Rec107p, a protein required for Spo11p-catalyzed DSB formation located on chromosome axes; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0008157 protein phosphatase 1 binding [PMID:11841235]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16537909]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:16537909]
EXISTENCE:mutant phenotype:GO:0043666 regulation of phosphoprotein phosphatase activity [PMID:16537909]
Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:12756240]
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:31532427]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10468587|PMID:15143178]
EXISTENCE:direct assay:GO:0008198 ferrous iron binding [PMID:12970193]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:31532427]
EXISTENCE:genetic interaction:GO:0002098 tRNA wobble uridine modification [PMID:17283054]
EXISTENCE:genetic interaction:GO:0016226 iron-sulfur cluster assembly [PMID:12970193]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10468587]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10588895|PMID:15143178|PMID:31040179]
Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
mitochondrial 2-oxodicarboxylate carrier
EXISTENCE:direct assay:GO:0005310 dicarboxylic acid transmembrane transporter activity [PMID:11013234]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9178508|PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11013234]
EXISTENCE:direct assay:GO:0006839 mitochondrial transport [PMID:11013234]
Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation; ODC1 has a paralog, ODC2, that arose from the whole genome duplication
gluconeogenesis transcription factor RDS2
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:17875938]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:17875938]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11943786|PMID:17875938]
EXISTENCE:mutant phenotype:GO:0061414 positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:17875938]
EXISTENCE:mutant phenotype:GO:0061415 negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:17875938]
EXISTENCE:mutant phenotype:GO:0071466 cellular response to xenobiotic stimulus [PMID:11943786]
Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:12063264]
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:2167832]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10501934|PMID:15776235]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0005761 mitochondrial ribosome [PMID:15776235]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:10617659]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:2167832]
Protein required for delivery of copper to Cox1p; mitochondrial inner membrane protein; involved in oxidative stress response; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
EXISTENCE:direct assay:GO:0008097 5S rRNA binding [PMID:8764831]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:8474444]
Ribosomal 60S subunit protein L5; nascent Rpl5p is bound by specific chaperone Syo1p during translation; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18756268]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
EXISTENCE:genetic interaction:GO:0051321 meiotic cell cycle [PMID:17179081]
EXISTENCE:genetic interaction:GO:0070583 spore membrane bending pathway [PMID:18756268]
EXISTENCE:mutant phenotype:GO:0070583 spore membrane bending pathway [PMID:18756268]
Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation; SPO19 has a paralog, YOR214C, that arose from the whole genome duplication
TATA-binding protein-associated factor TAF14
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:35970389]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0005674 transcription factor TFIIF complex [PMID:12242279|PMID:15896708|PMID:7995524]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:35970389|PMID:1331084]
EXISTENCE:direct assay:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:14749386]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:8187178]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:12887900]
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:10817755|PMID:17157260]
EXISTENCE:direct assay:GO:1990467 NuA3a histone acetyltransferase complex [PMID:25104842]
EXISTENCE:direct assay:GO:1990468 NuA3b histone acetyltransferase complex [PMID:25104842]
EXISTENCE:physical interaction:GO:0005674 transcription factor TFIIF complex [PMID:15896708]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; serves as an additional DNA binding domain for the complex; contains a YEATS domain
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:20513435]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:20513435]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:20513435]
EXISTENCE:direct assay:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:20513435]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0010833 telomere maintenance via telomere lengthening [PMID:17917674]
EXISTENCE:direct assay:GO:0031936 negative regulation of chromatin silencing [PMID:11258704]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:20513435]
EXISTENCE:direct assay:GO:0043035 chromatin insulator sequence binding [PMID:11258704]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:23222485]
EXISTENCE:genetic interaction:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:16467854]
EXISTENCE:mutant phenotype:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:20513435]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23222485]
Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation; involved in nucleosome displacement during double-strand break repair
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:22586276]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:11574687]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9046096|PMID:11914276]
EXISTENCE:genetic interaction:GO:0031936 negative regulation of chromatin silencing [PMID:19017647]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11574687]
EXISTENCE:mutant phenotype:GO:0030261 chromosome condensation [PMID:22586276]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:12820979]
EXISTENCE:mutant phenotype:GO:2000779 regulation of double-strand break repair [PMID:31821952]
Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
U3 snoRNP protein; component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA; localizes to nucleolus and peroxisomes
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16581791|PMID:10684247]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:20219973]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:20219973]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:20219973]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:20219973|PMID:16581791]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:20219973]
EXISTENCE:physical interaction:GO:0006606 protein import into nucleus [PMID:16581791]
Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:24390141]
EXISTENCE:direct assay:GO:0061493 central plaque of mitotic spindle pole body [PMID:10339566]
EXISTENCE:mutant phenotype:GO:1903087 mitotic spindle pole body duplication [PMID:10330408|PMID:10339566]
Inner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:24102742]
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:11158587]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11158587]
EXISTENCE:direct assay:GO:0005773 vacuole [PMID:19332891]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19332891|PMID:26928762]
EXISTENCE:genetic interaction:GO:0006915 apoptotic process [PMID:19332891]
EXISTENCE:mutant phenotype:GO:0000902 cell morphogenesis [PMID:11158587]
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:19332891]
EXISTENCE:mutant phenotype:GO:0006401 RNA catabolic process [PMID:19332891]
Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; required for tRNA-specific translational pausing during oxidative stress; promotes apoptosis under stress conditions and this function is independent of Rny1p catalytic activity
EXISTENCE:direct assay:GO:0000439 transcription factor TFIIH core complex [PMID:14500720|PMID:19818408]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:19897425]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408|PMID:27381459]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19818408]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:9235928]
EXISTENCE:physical interaction:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:10681587]
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:15579681|PMID:15620352]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0000707 meiotic DNA recombinase assembly [PMID:15579681|PMID:15620352]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15579681|PMID:15620352]
Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9105038]
EXISTENCE:direct assay:GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I [PMID:11157979]
EXISTENCE:direct assay:GO:0034272 phosphatidylinositol 3-kinase complex, class III, type II [PMID:11157979]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:12244127]
EXISTENCE:mutant phenotype:GO:0006661 phosphatidylinositol biosynthetic process [PMID:12244127]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:22437838]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11157979|PMID:32788210]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:21121900]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:9105038|PMID:9712845]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:12244127]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:11157979]
Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant
hypothetical protein; identified by expression profiling and mass spectrometry
putative DEAD-box ATP-dependent RNA helicase DBP1
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:31299079]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:31299079]
EXISTENCE:direct assay:GO:0033677 DNA/RNA helicase activity [PMID:21884706]
EXISTENCE:direct assay:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:31299079]
EXISTENCE:genetic interaction:GO:0006413 translational initiation [PMID:31299079]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:14763975]
ATP-dependent RNA helicase of the DEAD-box protein family; accelerates eIF4F-dependent assembly of 48S translation preinitiation complex (PIC), stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with DEAD-box RNA helicase and paralog Ded1p, stimulating PIC attachment and highly processive scanning by the 40S ribosomal subunit; protein abundance increases in response to DNA replication stress
mitochondrial 37S ribosomal protein MRP51
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769|PMID:9528754]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769|PMID:9528754]
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:9528754]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:9528754]
Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
isopentenyl-diphosphate delta-isomerase IDI1
EXISTENCE:direct assay:GO:0004452 isopentenyl-diphosphate delta-isomerase activity [PMID:2681212]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:2681212]
EXISTENCE:direct assay:GO:0045337 farnesyl diphosphate biosynthetic process [PMID:7858969]
Isopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; isopentenyl diphosphate:dimethylallyl diphosphate isomerase is also known as IPP isomerase
EXISTENCE:direct assay:GO:0004407 histone deacetylase activity [PMID:10535926]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:genetic interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17706600]
EXISTENCE:physical interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
ARS1617; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:17914457]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12455995|PMID:8227021|PMID:8300560|PMID:7498791]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17914457]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:17914457]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:17914457]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:17914457|PMID:19053807]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
EXISTENCE:genetic interaction:GO:0005096 GTPase activator activity [PMID:7498791]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:14517318]
EXISTENCE:genetic interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:17914457]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8013906]
EXISTENCE:mutant phenotype:GO:0035024 negative regulation of Rho protein signal transduction [PMID:17914457]
EXISTENCE:physical interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:17914457]
Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
EXISTENCE:mutant phenotype:GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor [PMID:17173333]
Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:12135984]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:12135984]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:12135984]
Peripheral peroxisomal membrane peroxin; required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with Pex27p; PEX25 has a paralog, PEX27, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0006413 translational initiation [PMID:9023104]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0071074 eukaryotic initiation factor eIF2 binding [PMID:14698289]
Methionine initiator tRNA (tRNA-Met); predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation
EXISTENCE:direct assay:GO:0004053 arginase activity [PMID:2404017]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:205532]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:1939179]
EXISTENCE:direct assay:GO:0019547 arginine catabolic process to ornithine [PMID:2404017]
EXISTENCE:direct assay:GO:0030145 manganese ion binding [PMID:1939179]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0090368 regulation of ornithine metabolic process [PMID:12679340]
EXISTENCE:direct assay:GO:0090369 ornithine carbamoyltransferase inhibitor activity [PMID:12679340]
EXISTENCE:mutant phenotype:GO:0004053 arginase activity [PMID:14582193]
EXISTENCE:mutant phenotype:GO:0019547 arginine catabolic process to ornithine [PMID:14582193]
EXISTENCE:mutant phenotype:GO:0090368 regulation of ornithine metabolic process [PMID:12679340]
EXISTENCE:mutant phenotype:GO:0090369 ornithine carbamoyltransferase inhibitor activity [PMID:12679340]
Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance
EXISTENCE:mutant phenotype:GO:0046475 glycerophospholipid catabolic process [PMID:16141200]
EXISTENCE:mutant phenotype:GO:0047389 glycerophosphocholine phosphodiesterase activity [PMID:16141200]
Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
UbiB family mitochondrial protein; involved in mobilization of CoQ from its site of synthesis on the inner mitochondrial membrane to other sites of action; contains transmembrane domain and mitochondrial targeting sequence
Cytoplasmic hypothetical protein; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
Putative mitochondrial hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; DPC25 is not an essential gene
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:17154545|PMID:16688212]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:17154545|PMID:15028727]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:16413483]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:16688212]
EXISTENCE:direct assay:GO:0042277 peptide binding [PMID:18539149]
EXISTENCE:genetic interaction:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:29507114]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:29507114]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:15028727]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:10480867]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:31962153]
ATPase component of heat shock protein Hsp90 chaperone complex; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; plays a role in prion propagation and determining prion variants; binds unfolded proteins; member of Hsp110 subclass of HSP70 proteins; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000932 P-body [PMID:17804396]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18327588|PMID:14562095]
EXISTENCE:genetic interaction:GO:0051664 nuclear pore localization [PMID:18327588]
hypothetical protein that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0070146 mitochondrial aspartyl-tRNA aminoacylation [PMID:10710420]
EXISTENCE:mutant phenotype:GO:0004815 aspartate-tRNA ligase activity [PMID:2668951]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:2668951]
EXISTENCE:mutant phenotype:GO:0070146 mitochondrial aspartyl-tRNA aminoacylation [PMID:2668951]
Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:21306442]
EXISTENCE:mutant phenotype:GO:0070291 N-acylethanolamine metabolic process [PMID:15878693]
EXISTENCE:mutant phenotype:GO:0070292 N-acylphosphatidylethanolamine metabolic process [PMID:15878693]
Protein with a role in maintaining mitochondrial morphology; also involved in maintaining normal cardiolipin levels; mitochondrial inner membrane protein; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:22343726]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:22343726]
EXISTENCE:direct assay:GO:0033588 elongator holoenzyme complex [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11689709]
Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity; null mutation is functionally complemented by human ELP4
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11852075|PMID:15194695|PMID:26928762]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:25691244]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18769150]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11852075]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:15155809|PMID:16876790]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:15155809]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:15103325]
EXISTENCE:mutant phenotype:GO:0000407 phagophore assembly site [PMID:25691244]
EXISTENCE:mutant phenotype:GO:0006497 protein lipidation [PMID:15155809]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:15155809]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:15194695]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:16876790]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:15155809|PMID:25691244]
Phosphoinositide binding protein; required for vesicle formation in cytoplasm-to-vacuole targeting (CVT) pathway, autophagy, micronucleophagy and mitophagy; binds to several phosphoinositides including PtdIns(3,5)P2, PI3P and PI4P; involved in PI3P-dependent recruitment and organization of both Atg12-Atg5-Atg16 complex and Atg8p at pre-autophagosomal structure; necessary for oxidant-induced cell death; targeted to vacuole via AP-3 pathway; WD-40 repeat containing PROPPIN family member
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239|PMID:26928762]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:24942160]
EXISTENCE:direct assay:GO:1990524 INA complex [PMID:24942160]
EXISTENCE:genetic interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:24942160]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:24942160]
EXISTENCE:physical interaction:GO:1990524 INA complex [PMID:24942160]
F1F0 ATPase synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Ina22p and ATP synthase subunits; null mutant displays elevated frequency of mitochondrial genome loss and has a respiratory growth defect
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:22613836]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:25454944]
EXISTENCE:physical interaction:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:22613836]
Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; provides quality control for insertion of membrane proteins by regulating their release into the inner membrane by the TIM23 complex; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
EXISTENCE:genetic interaction:GO:0004831 tyrosine-tRNA ligase activity [PMID:3315228]
EXISTENCE:genetic interaction:GO:0070184 mitochondrial tyrosyl-tRNA aminoacylation [PMID:3315228]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:3315228]
EXISTENCE:direct assay:GO:0005095 GTPase inhibitor activity [PMID:12832483]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12832483]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:15163411]
EXISTENCE:mutant phenotype:GO:0007265 Ras protein signal transduction [PMID:12832483]
EXISTENCE:physical interaction:GO:0000506 glycosylphosphatidylinositol-N- acetylglucosaminyltransferase (GPI-GnT) complex [PMID:15163411]
Endoplasmic reticulum membrane protein that binds and inhibits Ras2p; binds to and inhibits GTP-bound Ras2p at the endoplasmic reticulum (ER); component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase
EXISTENCE:direct assay:GO:0000224 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity [PMID:10831608]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10831608]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10831608|PMID:15910746]
EXISTENCE:direct assay:GO:0006517 protein deglycosylation [PMID:10831608]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:10831608]
EXISTENCE:mutant phenotype:GO:0006517 protein deglycosylation [PMID:25497214|PMID:27433019]
EXISTENCE:mutant phenotype:GO:0097466 ubiquitin-dependent glycoprotein ERAD pathway [PMID:27433019]
Conserved peptide N-glycanase; deglycosylating enzyme that cleaves N-glycans that are attached to misfolded ERAD substrate glycoproteins prior to proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p; human ortholog NGLY1 is associated with a syndrome characterized by developmental delays, epilepsy, absence of tears and liver disease
medium-chain fatty acid ethyl ester synthase/esterase
EXISTENCE:direct assay:GO:0034321 alcohol O-octanoyltransferase activity [PMID:16361250]
EXISTENCE:direct assay:GO:0034338 short-chain carboxylesterase activity [PMID:16361250]
EXISTENCE:direct assay:GO:0051792 medium-chain fatty acid biosynthetic process [PMID:16361250]
EXISTENCE:mutant phenotype:GO:0051792 medium-chain fatty acid biosynthetic process [PMID:16361250]
EXISTENCE:mutant phenotype:GO:0051793 medium-chain fatty acid catabolic process [PMID:16361250]
Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:27831485|PMID:26928762]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:physical interaction:GO:0031207 Sec62/Sec63 complex [PMID:2000150]
Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec66p, and Sec72p
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11112701]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:31909713]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:16861225|PMID:17443350|PMID:23212245]
EXISTENCE:direct assay:GO:0042273 ribosomal large subunit biogenesis [PMID:16888624]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:12773575]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12773575]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:17443350]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702|PMID:31909713]
Putative GTPase; associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
EXISTENCE:direct assay:GO:0000316 sulfite transport [PMID:10870099]
EXISTENCE:direct assay:GO:0000319 sulfite transmembrane transporter activity [PMID:10870099]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9294463]
Plasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein
EXISTENCE:direct assay:GO:0004362 glutathione-disulfide reductase (NADPH) activity [PMID:19049979|PMID:7737505]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23613772|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:23613772]
EXISTENCE:genetic interaction:GO:0010731 protein glutathionylation [PMID:20074363]
EXISTENCE:mutant phenotype:GO:0006749 glutathione metabolic process [PMID:23242256]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:8951820]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:23242256]
Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; also detected in peroxisomes; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9121433]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12410835]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:10064699]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:9121433]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9121433|PMID:12410835|PMID:15116342]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:7565726]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20398213|PMID:12410835]
EXISTENCE:mutant phenotype:GO:0060256 regulation of flocculation [PMID:31441971]
EXISTENCE:mutant phenotype:GO:1903450 regulation of G1 to G0 transition [PMID:31141453]
MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:31945465]
Putative aryl alcohol dehydrogenase; localizes to cytosol; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10900202]
EXISTENCE:direct assay:GO:0071633 dihydroceramidase activity [PMID:10900202]
EXISTENCE:genetic interaction:GO:0046513 ceramide biosynthetic process [PMID:11694577]
EXISTENCE:mutant phenotype:GO:0046514 ceramide catabolic process [PMID:10900202]
Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
ARS1618; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:10445034]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12139626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12139626]
Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin; human homolog ELP3 can partially complement yeast elp3 null mutant
EXISTENCE:direct assay:GO:0012507 ER to Golgi transport vesicle membrane [PMID:7593161]
EXISTENCE:direct assay:GO:0043495 protein-membrane adaptor activity [PMID:12235121|PMID:7593161]
EXISTENCE:direct assay:GO:0048208 COPII vesicle coating [PMID:12235121]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11694599]
EXISTENCE:mutant phenotype:GO:0070973 protein localization to endoplasmic reticulum exit site [PMID:22675024]
COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:34908216]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12668726]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12668726|PMID:15935782|PMID:34908216]
EXISTENCE:direct assay:GO:0035800 deubiquitinase activator activity [PMID:17446860]
EXISTENCE:direct assay:GO:2000158 positive regulation of ubiquitin-specific protease activity [PMID:17446860]
EXISTENCE:genetic interaction:GO:0016579 protein deubiquitination [PMID:15326198]
EXISTENCE:genetic interaction:GO:0070676 intralumenal vesicle formation [PMID:23444383]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:12062418]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:12668726]
EXISTENCE:mutant phenotype:GO:0007584 response to nutrient [PMID:8649366]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:34908216]
EXISTENCE:mutant phenotype:GO:0036010 protein localization to endosome [PMID:15326198]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:14523026]
EXISTENCE:mutant phenotype:GO:0070676 intralumenal vesicle formation [PMID:23444383]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:21070972]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27074665]
EXISTENCE:physical interaction:GO:0016579 protein deubiquitination [PMID:15326198]
Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
tRNA splicing endonuclease subunit SEN54
EXISTENCE:direct assay:GO:0000213 tRNA-intron endonuclease activity [PMID:9200603]
EXISTENCE:direct assay:GO:0000214 tRNA-intron endonuclease complex [PMID:9200603]
EXISTENCE:direct assay:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:9200603]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:12925762|PMID:16407407]
EXISTENCE:mutant phenotype:GO:0005741 mitochondrial outer membrane [PMID:12925762]
Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11880621]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:22298788]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21976730|PMID:22842922]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9234740]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9234740]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:17296733]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:17296733]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:17296733]
EXISTENCE:mutant phenotype:GO:0045892 negative regulation of DNA-templated transcription [PMID:24459134]
EXISTENCE:mutant phenotype:GO:0045898 regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15861138|PMID:21976730]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27226635]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:8083216]
Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA; coregulates transcription with Spt16p through assembly of preinitiation complex and organization of nucleosomes
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:16540469]
EXISTENCE:mutant phenotype:GO:0019843 rRNA binding [PMID:16540469]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains
Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000274 mitochondrial proton-transporting ATP synthase, stator stalk [PMID:18722382]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:8068646]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:8068646]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:18293929]
Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated
hypothetical protein; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
phosphatidylinositol N-acetylglucosaminyltransferase
EXISTENCE:curator inference:GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity []
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0006506 GPI anchor biosynthetic process [PMID:7768896]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:7768896]
Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10455229|PMID:12655409|PMID:1588965|PMID:1946357|PMI D:7808400|PMID:9271107|PMID:9335590]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9335590]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:12655409]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:1588965]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9271107]
EXISTENCE:mutant phenotype:GO:0034728 nucleosome organization [PMID:2854200]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10455229|PMID:15789351|PMID:9335590|PMID:11333224|PM ID:3547083|PMID:7808400|PMID:10510295]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:11333224]
EXISTENCE:mutant phenotype:GO:0061435 positive regulation of transcription from a mobile element promoter [PMID:9271107]
Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p; contains a long intron, which allows multiple RNA and protein isoforms with varying expression levels depending on glucose availability
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11553699]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:32639961]
Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
ARS1619; Autonomously Replicating Sequence
putative ubiquitin-specific protease UBP16
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:12914939|PMID:16407407]
Putative deubiquitinating enzyme anchored to the outer mitochondrial membrane; probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:23264632|PMID:23612966]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:23612966|PMID:26928762]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:23612966]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:23612966]
EXISTENCE:genetic interaction:GO:0036010 protein localization to endosome [PMID:23264632]
Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain
ARS1620; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0004311 farnesyltranstransferase activity [PMID:7665600]
EXISTENCE:direct assay:GO:0016114 terpenoid biosynthetic process [PMID:7665600]
EXISTENCE:mutant phenotype:GO:0016114 terpenoid biosynthetic process [PMID:7665600]
Geranylgeranyl diphosphate synthase (GGPPS); increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
EXISTENCE:mutant phenotype:GO:0097271 protein localization to bud neck [PMID:22344253]
EXISTENCE:physical interaction:GO:0097271 protein localization to bud neck [PMID:22344253]
Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
EXISTENCE:curator inference:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15086794]
EXISTENCE:direct assay:GO:0000813 ESCRT I complex [PMID:15044434]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:11511343]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:17215868]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:11511343]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:11511343]
EXISTENCE:physical interaction:GO:0000813 ESCRT I complex [PMID:16615894|PMID:11511343]
EXISTENCE:physical interaction:GO:0044877 protein-containing complex binding [PMID:17215868]
Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p
EXISTENCE:direct assay:GO:0005684 U2-type spliceosomal complex [PMID:11884590]
Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:12437925|PMID:12437924]
EXISTENCE:direct assay:GO:0030943 mitochondrion targeting sequence binding [PMID:18418384|PMID:19144822]
EXISTENCE:direct assay:GO:0046902 regulation of mitochondrial membrane permeability [PMID:16763150]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:16763150]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:12437924|PMID:12437925]
Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
EXISTENCE:mutant phenotype:GO:0032443 regulation of ergosterol biosynthetic process [PMID:30683746]
EXISTENCE:mutant phenotype:GO:0051485 terpenoid biosynthetic process, mevalonate-dependent [PMID:30683746]
EXISTENCE:mutant phenotype:GO:1904182 regulation of pyruvate dehydrogenase activity [PMID:30683746]
hypothetical protein; conserved across S. cerevisiae strains; deletion decreases transcription of downstream gene ALD6; YPL062W is not an essential gene; null mutant shows decrease in terpenoid production; homozygous diploid mutant shows a decrease in glycogen accumulation
EXISTENCE:direct assay:GO:0004030 aldehyde dehydrogenase [NAD(P)+] activity [PMID:9473035]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16806052|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16806052]
EXISTENCE:genetic interaction:GO:0006740 NADPH regeneration [PMID:12584194]
EXISTENCE:mutant phenotype:GO:0004030 aldehyde dehydrogenase [NAD(P)+] activity [PMID:9392076]
EXISTENCE:mutant phenotype:GO:0005829 cytosol [PMID:9392076]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:15146068]
EXISTENCE:mutant phenotype:GO:0019413 acetate biosynthetic process [PMID:11870853]
Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11254124]
EXISTENCE:mutant phenotype:GO:0015095 magnesium ion transmembrane transporter activity [PMID:11254124]
EXISTENCE:mutant phenotype:GO:0045016 mitochondrial magnesium ion transmembrane transport [PMID:11254124]
Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis
YPLCTy4-1; Ty4 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
TyA Gag protein; the main structural constituent of virus-like particles (VLPs); Gag processing produces capsid proteins
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11950925|PMID:15456753]
EXISTENCE:direct assay:GO:0015036 disulfide oxidoreductase activity [PMID:12730244]
EXISTENCE:direct assay:GO:0051537 2 iron, 2 sulfur cluster binding [PMID:23615440]
EXISTENCE:genetic interaction:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:11950925]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:10567543]
EXISTENCE:mutant phenotype:GO:0015036 disulfide oxidoreductase activity [PMID:10567543]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10567543]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:11950925|PMID:12970193]
EXISTENCE:mutant phenotype:GO:0106034 protein maturation by [2Fe-2S] cluster transfer [PMID:23615440]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:23615440]
Glutathione-dependent oxidoreductase; mitochondrial matrix protein involved at an early step in the biogenesis of iron-sulfur centers along with Bol1p; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p
ATP-binding cassette multidrug transporter PDR12
EXISTENCE:direct assay:GO:0005342 organic acid transmembrane transporter activity [PMID:10419965]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9687494]
EXISTENCE:direct assay:GO:0015849 organic acid transport [PMID:10419965]
Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
mannosylinositol phosphorylceramide synthase catalytic subunit SUR1
EXISTENCE:mutant phenotype:GO:0006675 mannosyl-inositol phosphorylceramide metabolic process [PMID:9323360]
EXISTENCE:mutant phenotype:GO:0006688 glycosphingolipid biosynthetic process [PMID:9323360]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:12954640]
EXISTENCE:mutant phenotype:GO:0103064 inositol phosphorylceramide mannosyltransferase activity [PMID:12954640]
Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
hypothetical protein; deletion mutant is fluconazole resistant and has long chronological lifespan
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15528187|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:18069899]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:12535538]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0016571 histone methylation [PMID:12535538]
EXISTENCE:mutant phenotype:GO:0016574 histone ubiquitination [PMID:12535538]
Protein involved in histone H2B ubiquitination; acts as cofactor for Bre1p; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
ARS1633; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:30842360]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9218445]
EXISTENCE:mutant phenotype:GO:0000031 mannosylphosphate transferase activity [PMID:9218445]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9218445]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:30842360]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9090056]
Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; facilitates the exit of Mnn4p from the ER via COPII-coated vesicles; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:15538383]
EXISTENCE:direct assay:GO:0008073 ornithine decarboxylase inhibitor activity [PMID:15538383]
EXISTENCE:direct assay:GO:0061136 regulation of proteasomal protein catabolic process [PMID:18089576]
EXISTENCE:mutant phenotype:GO:2001125 negative regulation of translational frameshifting [PMID:21900894]
Regulator of ornithine decarboxylase Spe1p; antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:9920936]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15077113|PMID:15077114]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:9920936]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:9920936]
EXISTENCE:mutant phenotype:GO:0034067 protein localization to Golgi apparatus [PMID:26966233|PMID:12620189]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:16926193]
ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1
mannosyltransferase complex subunit MNN9
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:19249370]
EXISTENCE:direct assay:GO:0000136 mannan polymerase complex [PMID:9430634]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:9430634]
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:19249370|PMID:9430634]
Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:10825185]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8918885|PMID:9094309]
EXISTENCE:direct assay:GO:1990526 Ste12p-Dig1p-Dig2p complex [PMID:16782869]
EXISTENCE:direct assay:GO:1990527 Tec1p-Ste12p-Dig1p complex [PMID:16782869]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9860980|PMID:9094309]
EXISTENCE:genetic interaction:GO:2000218 negative regulation of invasive growth in response to glucose limitation [PMID:8918885|PMID:9094309]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:8918885|PMID:9094309]
MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication
translation elongation factor EF1B gamma
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:12824466]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:3528160]
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:8465602]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:8465602]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12824466]
EXISTENCE:direct assay:GO:0006414 translational elongation [PMID:3528160]
EXISTENCE:genetic interaction:GO:0006449 regulation of translational termination [PMID:19545407]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:12824466]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12824466]
One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids
SAGA histone acetyltransferase complex subunit SGF11
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:15282323]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:18488019]
EXISTENCE:direct assay:GO:0071819 DUBm complex [PMID:20395473]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:15282323]
Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
EXISTENCE:direct assay:GO:0000113 nucleotide-excision repair factor 4 complex [PMID:15226437]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:16675952]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:16675952]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:16705154]
EXISTENCE:mutant phenotype:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:17296727]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:18817898]
EXISTENCE:physical interaction:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:16675952]
EXISTENCE:physical interaction:GO:0070449 elongin complex [PMID:10430890|PMID:10753924]
Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10944212|PMID:9362071]
EXISTENCE:direct assay:GO:0033263 CORVET complex [PMID:17488625]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:16601699]
EXISTENCE:direct assay:GO:0035542 regulation of SNARE complex assembly [PMID:18385512]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:direct assay:GO:0099022 vesicle tethering [PMID:27307091]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:9362071]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:11422941]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:9362071]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:11422941]
EXISTENCE:physical interaction:GO:0030897 HOPS complex [PMID:10944212]
Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages
mRNA-binding ribosome biosynthesis protein NOP4
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:21811236]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8039505]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:8039505]
Nucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs); human ortholog RBM28 implicated in ANE syndrome
CDK8; GIG2; NUT7; RYE5; SRB10; SSX7; UME5; URR1
cyclin-dependent serine/threonine protein kinase SSN3
EXISTENCE:direct assay:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:11331604]
EXISTENCE:direct assay:GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity [PMID:9702190]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:11383511|PMID:12200444]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:9702190]
EXISTENCE:genetic interaction:GO:0060258 negative regulation of filamentous growth [PMID:12520306]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16109375]
EXISTENCE:mutant phenotype:GO:0000435 positive regulation of transcription from RNA polymerase II promoter by galactose [PMID:15601835]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:31506296]
EXISTENCE:mutant phenotype:GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity [PMID:9702190]
EXISTENCE:mutant phenotype:GO:0031648 protein destabilization [PMID:12520306]
EXISTENCE:mutant phenotype:GO:0060258 negative regulation of filamentous growth [PMID:12520306]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:17660569]
EXISTENCE:mutant phenotype:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:9702190]
EXISTENCE:physical interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11226276]
Component of the RNA polymerase II mediator complex; cyclin-dependent protein kinase involved in phosphorylation of the mediator component Pgd1p and suppression of transcription activation by RNA polymerase II; involved in glucose repression
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
Protein that associates with mitochondrial ribosome; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; involved in maintenance of telomere length
EXISTENCE:curator inference:GO:0070152 mitochondrial isoleucyl-tRNA aminoacylation [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7607232]
Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
hypothetical protein; YPL039W is not an essential gene
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:9199298]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9199298]
EXISTENCE:direct assay:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:genetic interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9199298]
EXISTENCE:genetic interaction:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:22696683]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9199298]
EXISTENCE:mutant phenotype:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22696683]
EXISTENCE:physical interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005854 nascent polypeptide-associated complex [PMID:10219998]
EXISTENCE:genetic interaction:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:10518932]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:9482879]
Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8396147|PMID:16622836]
EXISTENCE:direct assay:GO:0008553 P-type proton-exporting transporter activity [PMID:8396147]
EXISTENCE:direct assay:GO:1902600 proton transmembrane transport [PMID:8396147]
Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
hypothetical protein; YPL034W is not essential gene
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0009200 deoxyribonucleoside triphosphate metabolic process [PMID:18337697]
hypothetical protein; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922|PMID:32491201]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:22842922|PMID:32491201]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:9751732]
hypothetical protein; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication
cyclin-dependent serine/threonine-protein kinase PHO85
EXISTENCE:direct assay:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:12407105|PMID:7973730|PMID:7973731|PMID:8108735|PMID :9584169|PMID:19823668]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9853758]
EXISTENCE:direct assay:GO:0019216 regulation of lipid metabolic process [PMID:31678512]
EXISTENCE:direct assay:GO:0032880 regulation of protein localization [PMID:8539622]
EXISTENCE:direct assay:GO:0046822 regulation of nucleocytoplasmic transport [PMID:31422915]
EXISTENCE:direct assay:GO:0055088 lipid homeostasis [PMID:31678512]
EXISTENCE:direct assay:GO:0071073 positive regulation of phospholipid biosynthetic process [PMID:27743455]
EXISTENCE:direct assay:GO:1901987 regulation of cell cycle phase transition [PMID:29146254]
EXISTENCE:direct assay:GO:1990860 Pho85-Pho80 CDK-cyclin complex [PMID:8108735]
EXISTENCE:genetic interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668|PMID:15738404]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:3915785]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:16964260]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:12077337]
EXISTENCE:genetic interaction:GO:0031647 regulation of protein stability [PMID:16611745]
EXISTENCE:genetic interaction:GO:0032878 regulation of establishment or maintenance of cell polarity [PMID:17853895]
EXISTENCE:genetic interaction:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:12101234]
EXISTENCE:genetic interaction:GO:0045936 negative regulation of phosphate metabolic process [PMID:3915785]
EXISTENCE:genetic interaction:GO:0050849 negative regulation of calcium-mediated signaling [PMID:16455487]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:mutant phenotype:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:9593297]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:16964260]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:20417603]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:20417603]
EXISTENCE:mutant phenotype:GO:0031647 regulation of protein stability [PMID:16508019]
EXISTENCE:mutant phenotype:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:16455487]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:7973730|PMID:7973731|PMID:8108735|PMID:9584169|PMID: 16611745]
Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle; human lissencephaly-associated homolog CDK5 functionally complements null mutation
EXISTENCE:direct assay:GO:0016300 tRNA (uracil) methyltransferase activity [PMID:18025252]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:18025252]
EXISTENCE:mutant phenotype:GO:0002128 tRNA nucleoside ribose methylation [PMID:32053677]
EXISTENCE:mutant phenotype:GO:0016300 tRNA (uracil) methyltransferase activity [PMID:18025252]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:18025252]
tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
EXISTENCE:direct assay:GO:0000262 mitochondrial chromosome [PMID:21911497]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:12426313|PMID:17658549]
EXISTENCE:direct assay:GO:0008859 exoribonuclease II activity [PMID:17658549]
EXISTENCE:direct assay:GO:0045025 mitochondrial degradosome [PMID:12426313]
EXISTENCE:genetic interaction:GO:0000372 Group I intron splicing [PMID:20064926]
EXISTENCE:mutant phenotype:GO:0000957 mitochondrial RNA catabolic process [PMID:12426313]
EXISTENCE:mutant phenotype:GO:0006264 mitochondrial DNA replication [PMID:21911497]
ATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron; expression of a processed form of human homolog SUPV3L1 carrying an N-terminal deletion of 46 amino acids rescues yeast suv3 null mutant
EXISTENCE:direct assay:GO:0003985 acetyl-CoA C-acetyltransferase activity [PMID:7989303|PMID:5571829]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:5571830]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:5571829]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:200835]
Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis; human ACAT1 functionally complements the growth defect caused by repression of ERG10 expression
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:16148007|PMID:24390141]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:16148007|PMID:11470404]
hypothetical protein involved in prospore membrane assembly; involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p
putative serine/threonine protein kinase SKS1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18417610]
EXISTENCE:genetic interaction:GO:0009749 response to glucose [PMID:9559544]
EXISTENCE:genetic interaction:GO:0010827 regulation of glucose transmembrane transport [PMID:8948096]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8948096]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:15899853]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:15899853]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:17693398]
EXISTENCE:direct assay:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15899853]
EXISTENCE:direct assay:GO:0043388 positive regulation of DNA binding [PMID:17693398]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:23036200]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:15889139]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:17571075]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:17571075|PMID:23036200]
EXISTENCE:physical interaction:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15889139]
Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase
methylenetetrahydrofolate reductase (NAD(P)H) MET12
EXISTENCE:direct assay:GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity [PMID:10600168]
Protein with MTHFR activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of methylenetetrahydrofolate reductase (MTHFR)
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:8253764]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:8253764]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9852079]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:7559571]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:11514439]
EXISTENCE:mutant phenotype:GO:0000735 removal of nonhomologous ends [PMID:1411547|PMID:7891718]
EXISTENCE:mutant phenotype:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:7891718|PMID:1411547]
EXISTENCE:mutant phenotype:GO:0006277 DNA amplification [PMID:23271978]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:3065620]
EXISTENCE:physical interaction:GO:0000110 nucleotide-excision repair factor 1 complex [PMID:8621533]
Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:11889490]
Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
ARS1621; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10713161|PMID:20647537]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:20647537]
EXISTENCE:direct assay:GO:0008234 cysteine-type peptidase activity [PMID:10094048]
EXISTENCE:direct assay:GO:0016926 protein desumoylation [PMID:10094048]
EXISTENCE:direct assay:GO:0016929 deSUMOylase activity [PMID:10094048]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12471376]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:10094048]
EXISTENCE:mutant phenotype:GO:0016926 protein desumoylation [PMID:10094048]
EXISTENCE:mutant phenotype:GO:0016929 deSUMOylase activity [PMID:10094048]
Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; contributes to regulation of telomeric silencing; sequestered to the nucleolus under stress conditions
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11102525|PMID:11823419|PMID:26928762]
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:17079729]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17079729]
EXISTENCE:direct assay:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:direct assay:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:genetic interaction:GO:0006797 polyphosphate metabolic process [PMID:11102525]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:11102525]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:11823419]
EXISTENCE:physical interaction:GO:0033254 vacuolar transporter chaperone complex [PMID:11823419]
Regulatory subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; targeted to vacuole via AP-3 pathway; VTC3 has a paralog, VTC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:10189365]
EXISTENCE:direct assay:GO:0000817 COMA complex [PMID:14633972]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10189365]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:10189365]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:10189365]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:22426531]
EXISTENCE:mutant phenotype:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:10189365]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:26653857]
Outer kinetochore protein, needed for accurate chromosome segregation; component of kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as platform for kinetochore assembly; required for spindle assembly checkpoint; minimizes potentially deleterious centromere-proximal crossovers by preventing meiotic DNA break formation proximal to centromere; homolog of human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:19285398]
EXISTENCE:direct assay:GO:0007020 microtubule nucleation [PMID:19285398]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:19285398]
EXISTENCE:direct assay:GO:0016651 oxidoreductase activity, acting on NAD(P)H [PMID:30267443]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:18085829]
EXISTENCE:mutant phenotype:GO:0034453 microtubule anchoring [PMID:19285398]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:18069899]
EXISTENCE:mutant phenotype:GO:0045931 positive regulation of mitotic cell cycle [PMID:19285398]
EXISTENCE:mutant phenotype:GO:0051315 attachment of mitotic spindle microtubules to kinetochore [PMID:19285398]
Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:3143101]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:8016655]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]
EXISTENCE:genetic interaction:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:14580348]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:10198436]
EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF complex [PMID:8159677]
EXISTENCE:mutant phenotype:GO:0031496 positive regulation of mating type switching [PMID:6436497]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1339306|PMID:3542227|PMID:8016655|PMID:10549298]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:14580348]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11865042|PMID:14580348]
Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; self-assembles to form [SWI+] prion and to alter expression pattern; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17110954]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11226170]
EXISTENCE:direct assay:GO:0017136 NAD-dependent histone deacetylase activity [PMID:10811920]
EXISTENCE:genetic interaction:GO:0045950 negative regulation of mitotic recombination [PMID:16051752]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:11226170|PMID:16051752]
EXISTENCE:mutant phenotype:GO:0017136 NAD-dependent histone deacetylase activity [PMID:10841563]
EXISTENCE:mutant phenotype:GO:0045950 negative regulation of mitotic recombination [PMID:16051752]
Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
EXISTENCE:direct assay:GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity [PMID:27005485]
EXISTENCE:genetic interaction:GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:27005485]
EXISTENCE:mutant phenotype:GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:27005485]
Cyclin-dependent kinase inhibitor; activated by environmental stress; interacts with and inhibits the Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex but not S-phase, or M-phase complexes; overexpression blocks cells in G1 phase and stabilizes the Cdc28p inhibitor Sic1p, while disruption accelerates the G1/S phase transition; phosphorylated during S phase in a Cdc28p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
mitochondrial 37S ribosomal protein MRPS16
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14729571]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]
Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; also plays a role in the cell cycle and the DNA damage response; contains HEAT-repeats
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:15448131]
EXISTENCE:curator inference:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10788514]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:17660549]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:15448131|PMID:10788514]
TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:21435344]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8617224]
EXISTENCE:mutant phenotype:GO:0030126 COPI vesicle coat [PMID:8617224]
Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:25554787]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:25554787]
EXISTENCE:direct assay:GO:1990112 RQC complex [PMID:23178123]
EXISTENCE:genetic interaction:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23479637]
EXISTENCE:mutant phenotype:GO:0043043 peptide biosynthetic process [PMID:25554787]
EXISTENCE:mutant phenotype:GO:0072344 rescue of stalled ribosome [PMID:26943317]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23479637]
EXISTENCE:physical interaction:GO:1990112 RQC complex [PMID:23479637]
Component of the Ribosome Quality Control (RQC) complex; RQC is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:15020404|PMID:17483413]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000785 chromatin [PMID:24086532]
EXISTENCE:mutant phenotype:GO:0003678 DNA helicase activity [PMID:10931920]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:15020404|PMID:14742714]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:34493312]
EXISTENCE:mutant phenotype:GO:0034085 establishment of sister chromatid cohesion [PMID:23334284]
EXISTENCE:physical interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:15020404]
Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
transcription factor TFIIIC subunit TFC8
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:10567530|PMID:2002052|PMID:1474578]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:3633923|PMID:8464480|PMID:2183033]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2002052]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:genetic interaction:GO:0006383 transcription by RNA polymerase III [PMID:10567530]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:10567530]
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16138904|PMID:14562095]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:31543266]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:31543266]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:14970192]
Vacuolar membrane protein involved with Npc2p in sterol trafficking; transits through biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to accumulation of high amounts of lipids observed in patients with Neimann-Pick C disease caused by loss-of-function mutations in human NPC1; targeted to vacuole via AP-3 pathway
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:19843529]
EXISTENCE:mutant phenotype:GO:0016071 mRNA metabolic process [PMID:14742425]
Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:22123866]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:16496001]
EXISTENCE:mutant phenotype:GO:0006469 negative regulation of protein kinase activity [PMID:15016821]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16496001]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:15016821]
Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
EXISTENCE:direct assay:GO:0019781 NEDD8 activating enzyme activity [PMID:9545234]
EXISTENCE:direct assay:GO:0045116 protein neddylation [PMID:9545234]
EXISTENCE:mutant phenotype:GO:0019781 NEDD8 activating enzyme activity [PMID:9531531]
EXISTENCE:mutant phenotype:GO:0045116 protein neddylation [PMID:9545234]
Protein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation
EXISTENCE:direct assay:GO:0000814 ESCRT II complex [PMID:12194858]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:7785322]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12194858]
Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
histone acetyltransferase catalytic subunit HAT1
EXISTENCE:direct assay:GO:0000123 histone acetyltransferase complex [PMID:8858151|PMID:9575221]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16612003]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:7559580|PMID:8858151]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14761951|PMID:9575221]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8858151|PMID:9575221]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:10982821|PMID:15099519]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:7559580|PMID:8858151|PMID:9575221]
EXISTENCE:physical interaction:GO:0000123 histone acetyltransferase complex [PMID:8858151]
Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex; uses the cofactor acetyl coenzyme A to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
EXISTENCE:direct assay:GO:0004108 citrate (Si)-synthase activity [PMID:17570335]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9140965|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006099 tricarboxylic acid cycle [PMID:17570335]
EXISTENCE:direct assay:GO:0050440 2-methylcitrate synthase activity [PMID:17570335]
EXISTENCE:genetic interaction:GO:0019629 propionate catabolic process, 2-methylcitrate cycle [PMID:17570335]
EXISTENCE:mutant phenotype:GO:0004108 citrate (Si)-synthase activity [PMID:17570335]
EXISTENCE:mutant phenotype:GO:0050440 2-methylcitrate synthase activity [PMID:17570335]
Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0019541 propionate metabolic process [PMID:11179416]
Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene
ARS1622; Autonomously Replicating Sequence
Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit
ARS1634; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1505513]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:1505513]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1505513]
Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11092862]
EXISTENCE:direct assay:GO:0019629 propionate catabolic process, 2-methylcitrate cycle [PMID:11092862]
EXISTENCE:direct assay:GO:0046421 methylisocitrate lyase activity [PMID:11092862]
2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:10412984|PMID:16357219]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16357219]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23246437]
EXISTENCE:genetic interaction:GO:0071459 protein localization to chromosome, centromeric region [PMID:16357219]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:10412984]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10412984]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:31617566]
EXISTENCE:mutant phenotype:GO:0051177 meiotic sister chromatid cohesion [PMID:10412984]
Meiosis-specific component of the sister chromatid cohesion complex; alpha-kleisin family member that maintains cohesion between homologous chromosomes during meiosis I until chromosome arm-associated Rec8p is cleaved by Esp1p; cohesion between sister chromatid centromeres is maintained until metaphase of meiosis II when it is cleaved by Esp1; independent of the role in cohesion, Rec8p promotes allelic collisions and prevents nonspecific chromosome interactions; homolog of S. pombe Rec8p
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:21586585]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11504737]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11504737]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11504737|PMID:16176797|PMID:16980434|PMID:28068993]
Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15030478]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:23872066]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:26448198|PMID:23872066]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11248676]
Zn2Cys6 family transcription factor; positively regulates sterol uptake under anaerobic conditions with SUT1; represses filamentation-inducing genes during non-starvation conditions; positively regulates mating along with SUT1 by repressing the expression of genes (PRR2, NCE102 and RHO5) which function as mating inhibitors; multicopy suppressor of mutations that cause low activity of the cAMP/PKA pathway; SUT2 has a paralog, SUT1, that arose from the whole genome duplication
DNA-directed RNA polymerase I core subunit RPA135
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9837969]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:2023944]
RNA polymerase I second largest subunit A135
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
hypothetical protein; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to cytosol; conserved among Saccharomyces sensu stricto species
EXISTENCE:direct assay:GO:0046964 3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity [PMID:24296033]
EXISTENCE:direct assay:GO:1902557 5'-adenylyl sulfate transmembrane transporter activity [PMID:24296033]
EXISTENCE:direct assay:GO:1902558 5'-adenylyl sulfate transmembrane transport [PMID:24296033]
EXISTENCE:direct assay:GO:1902559 3'-phospho-5'-adenylyl sulfate transmembrane transport [PMID:24296033]
Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:23012409]
Putative zinc finger protein; YPR013C is not an essential gene
hypothetical protein; conserved across S. cerevisiae strains; YPR014C is not an essential gene
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:18617996]
Putative zinc finger transcription factor; binds DNA in sequence-specific manner; overexpression causes a cell cycle delay or arrest
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11238882]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:17353896]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11238882]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:16188229|PMID:17443350|PMID:23212245]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:9891075]
EXISTENCE:genetic interaction:GO:0000054 ribosomal subunit export from nucleus [PMID:17353896]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:17353896]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238882]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238882]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:10206977|PMID:11238882]
Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:9218467]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9218467|PMID:26928762]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:9218467]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9218467]
EXISTENCE:genetic interaction:GO:0006892 post-Golgi vesicle-mediated transport [PMID:8429886|PMID:9218467]
EXISTENCE:mutant phenotype:GO:0006892 post-Golgi vesicle-mediated transport [PMID:8429886|PMID:9218467]
EXISTENCE:physical interaction:GO:0006892 post-Golgi vesicle-mediated transport [PMID:9218467]
Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:11782447]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:25602519]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9030688]
EXISTENCE:direct assay:GO:0006335 DNA replication-dependent chromatin assembly [PMID:9030687]
EXISTENCE:direct assay:GO:0033186 CAF-1 complex [PMID:9030687]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:16503640]
EXISTENCE:mutant phenotype:GO:0006335 DNA replication-dependent chromatin assembly [PMID:9030688]
Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including histone H3/H4 tetramerization, silencing at telomeres, mating type loci and rDNA, maintenance of kinetochore structure, deactivation of DNA damage checkpoint after DNA repair, chromatin dynamics during transcription, and repression of divergent noncoding transcription
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:15723534]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10559985|PMID:10704410]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10559985|PMID:10704410]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:15723534|PMID:13679365]
EXISTENCE:direct assay:GO:0009378 four-way junction helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:18657510|PMID:15723534]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0042555 MCM complex [PMID:12480933]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:direct assay:GO:0097373 MCM core complex [PMID:13679365]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:2044962]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:17895243]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10834843]
EXISTENCE:mutant phenotype:GO:0033260 nuclear DNA replication [PMID:2044962]
EXISTENCE:mutant phenotype:GO:0034214 protein hexamerization [PMID:31385807]
Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:10336613]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:10336613|PMID:9857174]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:19528297]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:16551625|PMID:17761666|PMID:20219971]
Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae
EXISTENCE:direct assay:GO:0005313 L-glutamate transmembrane transporter activity [PMID:14622413]
EXISTENCE:direct assay:GO:0015183 L-aspartate transmembrane transporter activity [PMID:14622413]
EXISTENCE:direct assay:GO:0015292 uniporter activity [PMID:14622413]
EXISTENCE:direct assay:GO:0015297 antiporter activity [PMID:14622413]
EXISTENCE:direct assay:GO:0015810 aspartate transmembrane transport [PMID:14622413]
EXISTENCE:direct assay:GO:0015813 L-glutamate transmembrane transport [PMID:14622413]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026036]
EXISTENCE:mutant phenotype:GO:0044271 cellular nitrogen compound biosynthetic process [PMID:14622413]
Mitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis; human homolog SLC25A13 complements yeast null mutant
Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS; overproduction of a truncation allele suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
EXISTENCE:direct assay:GO:0000123 histone acetyltransferase complex [PMID:11036083]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:16364921]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:19948887]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11036083]
EXISTENCE:mutant phenotype:GO:0043487 regulation of RNA stability [PMID:31694990]
EXISTENCE:mutant phenotype:GO:0045892 negative regulation of DNA-templated transcription [PMID:16364921]
EXISTENCE:mutant phenotype:GO:0060195 negative regulation of antisense RNA transcription [PMID:21248844]
Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8688560]
EXISTENCE:direct assay:GO:0031942 i-AAA complex [PMID:16267274]
EXISTENCE:direct assay:GO:0045041 protein import into mitochondrial intermembrane space [PMID:16966379]
EXISTENCE:genetic interaction:GO:0004176 ATP-dependent peptidase activity [PMID:8688560]
EXISTENCE:mutant phenotype:GO:0004176 ATP-dependent peptidase activity [PMID:15772085]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:22993211]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:18843051]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:27398316]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:16966379]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:16966379]
Catalytic subunit of i-AAA protease complex; complex is located in mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as nonconventional translocation motor to pull PNPase into intermembrane space; also has role in intermembrane space protein folding; mutation causes elevated rate of mitochondrial turnover; human homolog YME1L1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665|PMID:11839796]
EXISTENCE:direct assay:GO:0070985 transcription factor TFIIK complex [PMID:11839796|PMID:19818408]
EXISTENCE:genetic interaction:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:8230216]
EXISTENCE:mutant phenotype:GO:0006995 cellular response to nitrogen starvation [PMID:26567215]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:26567215]
EXISTENCE:mutant phenotype:GO:1905866 positive regulation of Atg1/ULK1 kinase complex assembly [PMID:26567215]
Cyclin partner of the cyclin-dependent kinase (CDK) Kin28p; regulates the activity of Kin28p, a TFIIH-associated carboxy-terminal domain (CTD) kinase that facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex, and has a minor role in RNAPII transcription; subunit of TFIIK, a TFIIH subassembly; human homolog CCNH allows growth of a yeast ccl1 temperature-sensitive mutant at restrictive temperature
EXISTENCE:direct assay:GO:0005993 trehalose catabolic process [PMID:8764988]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15128531]
EXISTENCE:direct assay:GO:0030287 cell wall-bounded periplasmic space [PMID:15128531]
EXISTENCE:mutant phenotype:GO:0000324 fungal-type vacuole [PMID:7502577]
EXISTENCE:mutant phenotype:GO:0004555 alpha,alpha-trehalase activity [PMID:7502577]
EXISTENCE:mutant phenotype:GO:0005993 trehalose catabolic process [PMID:7502577|PMID:8764988]
EXISTENCE:mutant phenotype:GO:0015771 trehalose transport [PMID:19016884]
Acid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress
hypothetical protein; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum, while the mCherry fusion protein localizes to the vacuole
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16469703|PMID:22842922|PMID:26928762|PMID:27831485]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11278413]
EXISTENCE:direct assay:GO:0071782 endoplasmic reticulum tubular network [PMID:16469703]
EXISTENCE:genetic interaction:GO:0007029 endoplasmic reticulum organization [PMID:19665976]
EXISTENCE:genetic interaction:GO:0032581 ER-dependent peroxisome organization [PMID:27129769]
EXISTENCE:genetic interaction:GO:0034976 response to endoplasmic reticulum stress [PMID:27117666]
EXISTENCE:genetic interaction:GO:0048309 endoplasmic reticulum inheritance [PMID:27117666]
EXISTENCE:genetic interaction:GO:0051292 nuclear pore complex assembly [PMID:19273614]
EXISTENCE:genetic interaction:GO:0071786 endoplasmic reticulum tubular network organization [PMID:16469703|PMID:18442980|PMID:18309084]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:11278413|PMID:12427979]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:11278413]
Reticulon-interacting protein; ER integral membrane protein involved in the generation of tubular ER morphology; promotes membrane curvature; forms tubules in vitro; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Yip1p to mediate membrane traffic and with Sey1p to maintain ER morphology; facilitates lipid exchange between the ER and mitochondria; forms ER foci upon DNA replication stress
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:11489214]
Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12444972]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:12444972]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:10679005]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:12444972]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:17157260]
EXISTENCE:physical interaction:GO:0016573 histone acetylation [PMID:17157260]
Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
ARS1623; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10402465|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10402465]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:16390997]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:22832273]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:10402465]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:10402465]
EXISTENCE:physical interaction:GO:0000149 SNARE binding [PMID:15964280]
EXISTENCE:physical interaction:GO:0007264 small GTPase mediated signal transduction [PMID:16390997]
EXISTENCE:physical interaction:GO:0030010 establishment of cell polarity [PMID:15964280]
Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004812 aminoacyl-tRNA ligase activity [PMID:16741232]
EXISTENCE:direct assay:GO:0006427 histidyl-tRNA aminoacylation [PMID:16741232]
EXISTENCE:genetic interaction:GO:0032543 mitochondrial translation [PMID:16777356]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:3521891]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:3521891]
EXISTENCE:mutant phenotype:GO:0006427 histidyl-tRNA aminoacylation [PMID:1459448]
Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11011149]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8127913|PMID:9726966]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9726966]
Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
EXISTENCE:direct assay:GO:0004356 glutamate-ammonia ligase activity [PMID:4156034]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0006542 glutamine biosynthetic process [PMID:6129248]
EXISTENCE:mutant phenotype:GO:0004356 glutamate-ammonia ligase activity [PMID:1347768]
Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; forms filaments of back-to-back stacks of cylindrical homo-decamers at low pH, leading to enzymatic inactivation and storage during states of advanced cellular starvation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
H(+)-transporting V1 sector ATPase subunit H
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:18708638]
Subunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:25127041]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:25127041]
Small (67 amino acids) protein involved in sporulation; localizes to the prospore membrane; phosphorylated during meiosis; a longer, 5'-extended mRNA is also transcribed beginning in mid-meiosis, regulated by two MSEs (middle sporulation elements), and includes an uORF of 15 codons in its 5'-UTR; evidence transcription is regulated by Pdr1p
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11584268]
EXISTENCE:direct assay:GO:0016971 flavin-linked sulfhydryl oxidase activity [PMID:11584268]
EXISTENCE:direct assay:GO:0016972 thiol oxidase activity [PMID:11313344]
EXISTENCE:direct assay:GO:0060904 regulation of protein folding in endoplasmic reticulum [PMID:11584268]
Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)
hypothetical protein; partially overlaps verified gene ERV2/YPR037C but doesn't share phenotypes; also overlaps dubious ORF YPR039W; null mutant displays increased levels of spontaneous Rad52p foci, increased biofilm information, different altered sensitivities to various chemcicals
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:11741537]
Tap42p-interacting protein; negative regulator of the TORC1 signaling pathway that activates the type 2A-related phosphatase Sit4p by binding and antagonizing Tap42p, a SIT4 inhibitor; proposed to be part of a feedback loop to amplify Sit4p activity when TORC1 is inactivated; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0001732 formation of cytoplasmic translation initiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:12008673]
EXISTENCE:direct assay:GO:0005092 GDP-dissociation inhibitor activity [PMID:20485439]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:9308967]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:22851688]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:15145951|PMID:22683627|PMID:16380131]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:11018020|PMID:15838098]
EXISTENCE:genetic interaction:GO:0045947 negative regulation of translational initiation [PMID:20485439]
EXISTENCE:mutant phenotype:GO:0005092 GDP-dissociation inhibitor activity [PMID:20485439]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:9308967]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:11331597|PMID:22851688]
EXISTENCE:mutant phenotype:GO:0042256 cytosolic ribosome assembly [PMID:11331597]
EXISTENCE:mutant phenotype:GO:0045947 negative regulation of translational initiation [PMID:20485439]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:22683627|PMID:9395514]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:15145951|PMID:22851688]
Translation initiation factor eIF5; functions as both a GTPase-activating protein (GAP) that stimulates the hydrolysis of GTP bound to eIF-2, as part of the 43S preinitiation complex, and as a GDP dissociation inhibitor (GDI) to prevent recycling of eIF2; human ortholog eIF5 complements the yeast null mutant
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21685478|PMID:15024427|PMID:23222640]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11101532]
PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication
Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:21149575]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:19061648]
Protein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11782448]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11782448]
Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
EXISTENCE:direct assay:GO:0004826 phenylalanine-tRNA ligase activity [PMID:16162501]
EXISTENCE:mutant phenotype:GO:0004826 phenylalanine-tRNA ligase activity [PMID:3029120]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:3029120]
EXISTENCE:mutant phenotype:GO:0070156 mitochondrial phenylalanyl-tRNA aminoacylation [PMID:3029120]
Mitochondrial phenylalanyl-tRNA synthetase; active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19194512]
EXISTENCE:direct assay:GO:0016651 oxidoreductase activity, acting on NAD(P)H [PMID:20802492]
EXISTENCE:mutant phenotype:GO:0006809 nitric oxide biosynthetic process [PMID:23159617]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:20802492]
EXISTENCE:mutant phenotype:GO:0045429 positive regulation of nitric oxide biosynthetic process [PMID:27178802]
EXISTENCE:mutant phenotype:GO:1901300 positive regulation of hydrogen peroxide-mediated programmed cell death [PMID:27178802]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27178802]
Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance; possible target for development of antifungal drugs
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:15659643]
EXISTENCE:direct assay:GO:0006914 autophagy [PMID:28013049]
EXISTENCE:direct assay:GO:0034497 protein localization to phagophore assembly site [PMID:23858448|PMID:26774783]
EXISTENCE:direct assay:GO:0140255 regulation of cellular response to phosphate starvation [PMID:28013049]
EXISTENCE:genetic interaction:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:15659643]
EXISTENCE:genetic interaction:GO:0060090 molecular adaptor activity [PMID:17295840]
EXISTENCE:genetic interaction:GO:2000786 positive regulation of autophagosome assembly [PMID:18077553]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:11309418]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:12479808]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:15659643]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:17135291]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:27659307]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
EXISTENCE:physical interaction:GO:0034497 protein localization to phagophore assembly site [PMID:23858448]
Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy
EXISTENCE:direct assay:GO:0031417 NatC complex [PMID:10504710]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:11274203]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:11274203]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:15077113|PMID:15077114|PMID:15911569|PMID:17261844]
Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT); involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus; human NatC ortholog, NAA30, functionally complements the null, requiring either auxiliary subunit Mak10p or co-expression of human ortholog, NAA35; NAA60, the human NatF gene, also complements the null allele
high-mobility group nucleosome-binding protein
EXISTENCE:curator inference:GO:0006298 mismatch repair [PMID:19843605]
EXISTENCE:direct assay:GO:0001195 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III [PMID:16407207]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17878171|PMID:3169249]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:19683499]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:7721780|PMID:9468494]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:11432837|PMID:12805231|PMID:12952948]
EXISTENCE:direct assay:GO:0032407 MutSalpha complex binding [PMID:19843605]
EXISTENCE:direct assay:GO:0043392 negative regulation of DNA binding [PMID:19843605]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:8946917]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11239460|PMID:16407207|PMID:17178828]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:14739928]
EXISTENCE:genetic interaction:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11533232]
High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains; YPR053C is not an essential gene; partially overlaps verified ORF NHP6A/YPR052C
EXISTENCE:direct assay:GO:0004707 MAP kinase activity [PMID:16116083]
EXISTENCE:direct assay:GO:0070056 prospore membrane leading edge [PMID:33466572]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:16116083]
EXISTENCE:genetic interaction:GO:0042174 negative regulation of sporulation resulting in formation of a cellular spore [PMID:16116083]
EXISTENCE:mutant phenotype:GO:0004707 MAP kinase activity [PMID:16116083]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:16116083|PMID:7958885]
Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for prospore membrane development and the production of outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p; activated by Cak1p-mediated phosphorylation of threonine (T207) in the activation loop during meiosis I followed by Ssp2p-promoted autophosphorylation of tyrosine (T209) during meiosis II
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9491896]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:1512289|PMID:7615633|PMID:8978675]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12960429|PMID:9491896|PMID:22842922]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:15596717]
EXISTENCE:mutant phenotype:GO:0048313 Golgi inheritance [PMID:15596717]
Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:19818408]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:10506223]
Subunit of TFIIH complex; involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
EXISTENCE:direct assay:GO:0017069 snRNA binding [PMID:8861964]
EXISTENCE:direct assay:GO:0030532 small nuclear ribonucleoprotein complex [PMID:8861964]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:8861964]
snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23509072]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16140254|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0005342 organic acid transmembrane transporter activity [PMID:16140254]
EXISTENCE:genetic interaction:GO:0006783 heme biosynthetic process [PMID:26821380]
EXISTENCE:genetic interaction:GO:0006839 mitochondrial transport [PMID:16140254]
EXISTENCE:genetic interaction:GO:0015187 glycine transmembrane transporter activity [PMID:26821380]
EXISTENCE:genetic interaction:GO:1904983 glycine import into mitochondrion [PMID:26821380]
Secondary mitochondrial inner membrane glycine transporter; required with HEM25 for the transport of glycine into mitochondria for the initiation of heme biosynthesis; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; localizes to the vacuole in response to H2O2; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004106 chorismate mutase activity [PMID:3027508]
EXISTENCE:mutant phenotype:GO:0006571 tyrosine biosynthetic process [PMID:3027508]
EXISTENCE:mutant phenotype:GO:0009094 L-phenylalanine biosynthetic process [PMID:3027508]
Chorismate mutase; catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:19682992]
Probable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
EXISTENCE:direct assay:GO:0004131 cytosine deaminase activity [PMID:15535715|PMID:9000374]
EXISTENCE:mutant phenotype:GO:0008655 pyrimidine-containing compound salvage [PMID:9000374]
EXISTENCE:mutant phenotype:GO:0019858 cytosine metabolic process [PMID:9000374]
EXISTENCE:mutant phenotype:GO:0046087 cytidine metabolic process [PMID:10501935]
Cytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
hypothetical protein; conserved among S. cerevisiae strains; YPR064W is not an essential gene
EXISTENCE:curator inference:GO:0000785 chromatin [PMID:10446242|PMID:10487921|PMID:7565763|PMID:8413209]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:10487921]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:10446242|PMID:7565763]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:10446242|PMID:8413209]
EXISTENCE:genetic interaction:GO:0071470 cellular response to osmotic stress [PMID:23435728]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10446242|PMID:10487921|PMID:12051835|PMID:12095998|P MID:1588959|PMID:16835445|PMID:2546055|PMID:2847035|PMID:3 062365|PMID:9388292|PMID:21299653]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10446242]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:14665463]
EXISTENCE:mutant phenotype:GO:0071470 cellular response to osmotic stress [PMID:23435728]
Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
Protein that activates Rub1p (NEDD8) before neddylation; acts together with Ula1p; may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:21987576]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:10805735]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10913600]
EXISTENCE:genetic interaction:GO:0009102 biotin biosynthetic process [PMID:17259550]
EXISTENCE:genetic interaction:GO:0016226 iron-sulfur cluster assembly [PMID:10805735]
EXISTENCE:mutant phenotype:GO:0009102 biotin biosynthetic process [PMID:17259550]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10805735]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:10913600]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:21987576]
Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
EXISTENCE:direct assay:GO:0033558 protein lysine deacetylase activity [PMID:20832720|PMID:20797861]
EXISTENCE:mutant phenotype:GO:0033558 protein lysine deacetylase activity [PMID:20797861|PMID:20832720]
EXISTENCE:physical interaction:GO:0000118 histone deacetylase complex [PMID:22543816]
Class I histone deacetylase (HDAC) family member; deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex
ARS1624; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0004766 spermidine synthase activity [PMID:9073064]
EXISTENCE:mutant phenotype:GO:0008295 spermidine biosynthetic process [PMID:9073064]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9892641]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9892641]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Putative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:9023104]
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11889048]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:26952104]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16926149]
EXISTENCE:mutant phenotype:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:15706350]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:26952104]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:10490603]
Component of the CCR4-NOT core complex, involved in mRNA decapping; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:15358193|PMID:7629177]
EXISTENCE:direct assay:GO:0006470 protein dephosphorylation [PMID:7629177]
EXISTENCE:mutant phenotype:GO:0004725 protein tyrosine phosphatase activity [PMID:7629177]
Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:8299150]
EXISTENCE:mutant phenotype:GO:0004802 transketolase activity [PMID:8226984]
EXISTENCE:mutant phenotype:GO:0006098 pentose-phosphate shunt [PMID:8299150]
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
ARS1625; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16543225|PMID:22842922]
EXISTENCE:genetic interaction:GO:0007231 osmosensory signaling pathway [PMID:16543225]
EXISTENCE:genetic interaction:GO:0072659 protein localization to plasma membrane [PMID:17627274|PMID:22904036]
EXISTENCE:mutant phenotype:GO:0000751 mitotic cell cycle G1 arrest in response to pheromone [PMID:9383053]
EXISTENCE:mutant phenotype:GO:1900436 positive regulation of filamentous growth of a population of unicellular organisms in response to starvation [PMID:22904036]
EXISTENCE:physical interaction:GO:0007231 osmosensory signaling pathway [PMID:16543225]
Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
hypothetical protein; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11470415]
EXISTENCE:genetic interaction:GO:0005048 signal sequence binding [PMID:11470415]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:11470415]
Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
translation elongation factor EF-1 alpha
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:25561732]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:17925388]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20032305]
EXISTENCE:direct assay:GO:0019003 GDP binding [PMID:17925388]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:25561732]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:16116436|PMID:19095653|PMID:25561732]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:genetic interaction:GO:0006409 tRNA export from nucleus [PMID:10766739|PMID:17203074]
EXISTENCE:mutant phenotype:GO:0003746 translation elongation factor activity [PMID:10514524]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:10766739|PMID:17203074]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:10514524]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:16116436]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus
EXISTENCE:direct assay:GO:0004820 glycine-tRNA ligase activity [PMID:22438917|PMID:21877692]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21877692|PMID:14562095]
EXISTENCE:genetic interaction:GO:0004820 glycine-tRNA ligase activity [PMID:22438917]
EXISTENCE:genetic interaction:GO:0006426 glycyl-tRNA aminoacylation [PMID:22438917]
Glycine-tRNA synthetase, not expressed under normal growth conditions; expression is induced under heat, oxidative, pH, or ethanol stress conditions; more stable than the major glycine-tRNA synthetase Grs1p at 37 deg C; GRS2 has a paralog, GRS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10610776|PMID:11720284]
17-kDa component of the U4/U6aU5 tri-snRNP; plays an essential role in pre-mRNA splicing; human ortholog TXNL4A (the human U5-specific 15-kDa protein) complements yeast dib1 null mutant
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20505073]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:20505073]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:23641071]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:20505073]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:20505073]
Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-anchor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:7935466]
EXISTENCE:direct assay:GO:0001139 RNA polymerase II complex recruiting activity [PMID:10464320]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15888545]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:15888545|PMID:10464320]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10464320]
EXISTENCE:direct assay:GO:0097550 transcription preinitiation complex [PMID:22751016]
EXISTENCE:direct assay:GO:2000679 positive regulation of transcription regulatory region DNA binding [PMID:8132551]
EXISTENCE:mutant phenotype:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:19820686]
EXISTENCE:mutant phenotype:GO:0001139 RNA polymerase II complex recruiting activity [PMID:10464320]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:19820686]
EXISTENCE:mutant phenotype:GO:0017025 TBP-class protein binding [PMID:10464320]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10464320|PMID:9887099]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:7935466]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:15888545]
Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
RNA-binding signal recognition particle subunit SRP54
EXISTENCE:direct assay:GO:0008312 7S RNA binding [PMID:14681587]
EXISTENCE:mutant phenotype:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:1655273]
Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
hypothetical protein; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
EXISTENCE:direct assay:GO:0005319 lipid transporter activity [PMID:28011845]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:22250200]
EXISTENCE:direct assay:GO:0033116 endoplasmic reticulum-Golgi intermediate compartment membrane [PMID:28011845]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:22250200]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:28011845]
Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); involved in nonvesicular transfer of ceramides from ER to Golgi; may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U2729 and small subunit (SSU) rRNA at position A100
C/D box small nucleolar RNA (snoRNA); predicted to guide 2'-O-methylation of small subunit (SSU) rRNA at position C1639
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114|PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:8674114|PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at positions A541 and G1126
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:19064926]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15117954]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15117954]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:19064926]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:25646695]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:19064926]
EXISTENCE:mutant phenotype:GO:0045471 response to ethanol [PMID:15117954]
Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; has a role in organization of septins and the actin cytoskeleton; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:14517302]
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:15565172|PMID:16314500]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:12871902]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:14517302]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:12871902]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:11943786]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:16314500]
Component of the SF3b subcomplex of the U2 snRNP; zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
Arf family guanine nucleotide exchange factor SYT1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:20841378]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10430582]
EXISTENCE:direct assay:GO:0032014 positive regulation of ARF protein signal transduction [PMID:20841378]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:10430582]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:10430582]
EXISTENCE:mutant phenotype:GO:0032014 positive regulation of ARF protein signal transduction [PMID:20841378]
EXISTENCE:mutant phenotype:GO:0034067 protein localization to Golgi apparatus [PMID:26966233]
Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
hypothetical protein; may interact with ribosomes, based on co-purification experiments
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:11557775]
Phosphoinositide-binding regulator of ergosterol distribution; contains PX (Phox homology) domain; associated with ER-lipid droplet contact site; may facilitate ergosterol transport between plasma membrane and lipid droplets
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
Mitochondrial hypothetical protein; localized to the mitochondrial outer membrane
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31374566]
Mitochondrial hypothetical protein; null mutant has a respiratory growth defect and displays reduced peroxide-induced mitochondrial ROS; partially overlaps the verified gene MRPL51/YPR100W
mitochondrial 54S ribosomal protein MRPL51
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:31374566]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:31374566]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:31374566]
Mitochondrial ribosomal protein of the large subunit; required for mitochondrial genome integrity, respiratory growth, and mitochondrial redox homeostasis, partially overlaps verified gene YPR099C
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:9528791]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:9528791]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:9528791]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:9528791]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9528791]
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:2277060]
Ribosomal 60S subunit protein L11A; expressed at twice the level of Rpl11Bp; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11A has a paralog, RPL11B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:9087403]
EXISTENCE:curator inference:GO:0034515 proteasome storage granule [PMID:9087403]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:mutant phenotype:GO:0004175 endopeptidase activity [PMID:8808631|PMID:9312134]
EXISTENCE:mutant phenotype:GO:0080129 proteasome core complex assembly [PMID:8808631]
Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome; localizes to peroxisomes in oleate-growing cells
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:15620355|PMID:15692568]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:27016735]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15466158]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15466158]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
EXISTENCE:genetic interaction:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:7785326]
EXISTENCE:genetic interaction:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15616569]
EXISTENCE:mutant phenotype:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15620355]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15616569|PMID:15620355]
EXISTENCE:physical interaction:GO:0001222 transcription corepressor binding [PMID:15620355]
EXISTENCE:physical interaction:GO:0001223 transcription coactivator binding [PMID:15620355]
Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
EXISTENCE:mutant phenotype:GO:0000301 retrograde transport, vesicle recycling within Golgi [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0017119 Golgi transport complex [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20065092]
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
EXISTENCE:genetic interaction:GO:0018105 peptidyl-serine phosphorylation [PMID:32579556]
EXISTENCE:genetic interaction:GO:0018107 peptidyl-threonine phosphorylation [PMID:32579556]
EXISTENCE:genetic interaction:GO:0106279 negative regulation of UDP-N-acetylglucosamine biosynthetic process [PMID:32579556]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:32579556]
EXISTENCE:mutant phenotype:GO:0018107 peptidyl-threonine phosphorylation [PMID:32579556]
EXISTENCE:mutant phenotype:GO:0106279 negative regulation of UDP-N-acetylglucosamine biosynthetic process [PMID:32579556]
Predicted protein kinase; negatively regulates the hexosamine biosynthetic pathway (HBP), which converts fructose-6-phosphate into UDP-N-acetylglucosamine, a precursor of chitin biosynthesis, GPI-anchor formation and glycosylation; interacts genetically with GFA1, encoding the first enzyme in the HBP; involved in Gfa1p phosphorylation (S332, T334); contains a Pho85p-regulated SCF-CDC4 phosphodegron; unstable, cell cycle regulated protein, peaking at G1/S
cleavage polyadenylation factor RNA-binding subunit YTH1
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9224719]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:10899131]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:10899131]
Essential RNA-binding component of cleavage and polyadenylation factor; contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation; relocalizes to the cytosol in response to hypoxia
proteasome regulatory particle lid subunit RPN7
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19446323]
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:9741626]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:15102831]
Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0044695 Dsc E3 ubiquitin ligase complex [PMID:29355480]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:29355480]
Component of Dsc E3 ligase complex in Golgi and endosomes; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; diploid deletion strain has high budding index
DNA-directed RNA polymerase core subunit RPC40
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:mutant phenotype:GO:0070893 transposon integration [PMID:25931562]
RNA polymerase subunit AC40; common to RNA polymerase I and III; predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8131744]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:12937277]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12937277]
EXISTENCE:genetic interaction:GO:0000281 mitotic cytokinesis [PMID:12937277]
EXISTENCE:genetic interaction:GO:0010458 exit from mitosis [PMID:1916278]
EXISTENCE:mutant phenotype:GO:0000917 division septum assembly [PMID:17005296]
EXISTENCE:mutant phenotype:GO:0061013 regulation of mRNA catabolic process [PMID:22196726]
Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication
ARS1626; Autonomously Replicating Sequence
RNA-binding ribosome biosynthesis protein MRD1
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11884397]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:23193268|PMID:12150911]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:23193268]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11884397]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11884397]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11884397]
EXISTENCE:mutant phenotype:GO:0034462 small-subunit processome assembly [PMID:18586827]
Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
CDP-diacylglycerol--inositol 3-phosphatidyltransferase
EXISTENCE:direct assay:GO:0003881 CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity [PMID:3005284]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:3005242]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8543066]
EXISTENCE:direct assay:GO:0006661 phosphatidylinositol biosynthetic process [PMID:3005284]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:6274497]
EXISTENCE:mutant phenotype:GO:0003881 CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity [PMID:6088233]
EXISTENCE:mutant phenotype:GO:0006661 phosphatidylinositol biosynthetic process [PMID:6088233]
Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
Protein distantly similar to ceramide synthases, Lag1p and Lac1p; mutation confers sensitivity to inhibitors of sphingolipid biosynthesis
EXISTENCE:mutant phenotype:GO:0090372 positive regulation of glycerol transport [PMID:19956799]
Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0099617 matrix side of mitochondrial inner membrane [PMID:30759361]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:19751518]
EXISTENCE:mutant phenotype:GO:0097745 mitochondrial tRNA 5'-end processing [PMID:30759361]
hypothetical protein; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport; localizes to the matrix side of the inner mitochondrial membrane
Modulator of cellular lipid distribution; localizes to ER-PM and ER-mitochondria contact sites; shares homology with lipid transfer proteins Vps13p and Atg2p; homolog of D. melanogaster Hobbit protein
S-methyl-5-thioribose-1-phosphate isomerase MRI1
EXISTENCE:direct assay:GO:0046523 S-methyl-5-thioribose-1-phosphate isomerase activity [PMID:15215245]
EXISTENCE:mutant phenotype:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:15215245|PMID:18625006]
5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11171327|PMID:12972503]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12972503]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:12972503]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:12972503]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12972503]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:1731251]
EXISTENCE:direct assay:GO:0035308 negative regulation of protein dephosphorylation [PMID:10580002]
EXISTENCE:direct assay:GO:0045737 positive regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:27068241]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:1387566]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:1849457]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8455600]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:11171327]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:1731251|PMID:1849457]
EXISTENCE:mutant phenotype:GO:0032888 regulation of mitotic spindle elongation [PMID:23468650]
EXISTENCE:mutant phenotype:GO:0045737 positive regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:27068241]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:22918234]
B-type cyclin involved in cell cycle progression; also involved in response to DNA damage; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7954792]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10848575]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:7954792]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8253070]
EXISTENCE:genetic interaction:GO:0006279 premeiotic DNA replication [PMID:9732268]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:11438663|PMID:16688214]
EXISTENCE:genetic interaction:GO:0045740 positive regulation of DNA replication [PMID:8319908]
EXISTENCE:genetic interaction:GO:1901673 regulation of mitotic spindle assembly [PMID:8319908]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8253070|PMID:8319908]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8319908]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:9732268]
EXISTENCE:mutant phenotype:GO:0045740 positive regulation of DNA replication [PMID:8319908|PMID:9734354]
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication
ARS1635; Autonomously Replicating Sequence
EXISTENCE:expression pattern:GO:0009228 thiamine biosynthetic process [PMID:10383756]
Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12176366]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12176366]
EXISTENCE:genetic interaction:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:7569998]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9744878]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:7569998]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:7990931]
EXISTENCE:mutant phenotype:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:7569998]
Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7929270|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0005375 copper ion transmembrane transporter activity [PMID:7929270]
EXISTENCE:mutant phenotype:GO:0015677 copper ion import [PMID:7929270]
High-affinity copper transporter of plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress; human homolog SLC31A1 can complement a yeast ctr1 ctr3 double deletion
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24390141|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:16476776]
EXISTENCE:genetic interaction:GO:0006813 potassium ion transport [PMID:20197279]
EXISTENCE:genetic interaction:GO:1902600 proton transmembrane transport [PMID:20197279]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:16476776]
Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome
Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
EXISTENCE:direct assay:GO:0000295 adenine nucleotide transmembrane transporter activity [PMID:11566870]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:11566870]
EXISTENCE:direct assay:GO:0015867 ATP transport [PMID:11566870]
EXISTENCE:mutant phenotype:GO:0000295 adenine nucleotide transmembrane transporter activity [PMID:11390660]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:11390660|PMID:11566870]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:11390660]
Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
EXISTENCE:direct assay:GO:0000932 P-body [PMID:22284680]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:22284680]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:22284680|PMID:26777405]
EXISTENCE:direct assay:GO:0031370 eukaryotic initiation factor 4G binding [PMID:22284680]
EXISTENCE:direct assay:GO:0045947 negative regulation of translational initiation [PMID:20832728]
EXISTENCE:mutant phenotype:GO:0033962 P-body assembly [PMID:22284680]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:22284680]
EXISTENCE:mutant phenotype:GO:0045947 negative regulation of translational initiation [PMID:20832728]
Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
peptide alpha-N-acetyltransferase complex B subunit NAT3
EXISTENCE:direct assay:GO:0031416 NatB complex [PMID:12783868]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:12808144]
EXISTENCE:genetic interaction:GO:0032956 regulation of actin cytoskeleton organization [PMID:12808144]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:12808144]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:10545125]
EXISTENCE:mutant phenotype:GO:0017196 N-terminal peptidyl-methionine acetylation [PMID:10545125]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:23363603]
EXISTENCE:mutant phenotype:GO:0032956 regulation of actin cytoskeleton organization [PMID:12808144]
Catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
EXISTENCE:genetic interaction:GO:0006450 regulation of translational fidelity [PMID:8415737|PMID:8950190]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:8415737|PMID:8950190]
Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:12524336|PMID:12556496|PMID:18086892]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:29300974]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:12524336|PMID:12556496]
EXISTENCE:genetic interaction:GO:0034243 regulation of transcription elongation by RNA polymerase II [PMID:18086892]
EXISTENCE:mutant phenotype:GO:0006334 nucleosome assembly [PMID:29300974]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:19034519]
EXISTENCE:mutant phenotype:GO:0031060 regulation of histone methylation [PMID:32941642]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:12556496|PMID:18086892]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:12556496]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12556496]
Protein involved in RNA polymerase II transcription; also required for histone modifications and splicing; constitutively recruited to the CYC1 promoter and required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain is highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:9217162|PMID:16407407]
EXISTENCE:direct assay:GO:0005742 mitochondrial outer membrane translocase complex [PMID:9217162]
EXISTENCE:direct assay:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:20668160]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:9217162]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:9217162]
Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore
EXISTENCE:curator inference:GO:0005739 mitochondrion [PMID:2842150]
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:2842150]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:2842150]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:21257754]
Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11287619]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:20381454]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16962805]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:17483413]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:1448101]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:20980819]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:1341195|PMID:1448101]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:11287619]
Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p); recruits Mms22p to replication forks during S phase, and may link DNA synthesis to sister chromatid cohesion
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12150911]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:11081632]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:23509373]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11105764]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11105764]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11105764]
Protein involved in pre-rRNA processing; associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YPRCTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:9234685]
Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
lysophosphatidic acid acyltransferase LOA1
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:22090344|PMID:26928762]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:21820081|PMID:22090344]
EXISTENCE:direct assay:GO:0042171 lysophosphatidic acid acyltransferase activity [PMID:22090344]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:22090344]
EXISTENCE:mutant phenotype:GO:0035356 cellular triglyceride homeostasis [PMID:22090344]
Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16135531|PMID:16880272]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:16880272]
EXISTENCE:direct assay:GO:0035965 cardiolipin acyl-chain remodeling [PMID:27268057]
EXISTENCE:direct assay:GO:0047184 1-acylglycerophosphocholine O-acyltransferase activity [PMID:27268057]
EXISTENCE:mutant phenotype:GO:0007007 inner mitochondrial membrane organization [PMID:14651618]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:14651618|PMID:15588229]
EXISTENCE:mutant phenotype:GO:0032048 cardiolipin metabolic process [PMID:14651618]
EXISTENCE:mutant phenotype:GO:0035965 cardiolipin acyl-chain remodeling [PMID:14651618]
EXISTENCE:mutant phenotype:GO:0042775 mitochondrial ATP synthesis coupled electron transport [PMID:15304507]
EXISTENCE:mutant phenotype:GO:0047184 1-acylglycerophosphocholine O-acyltransferase activity [PMID:15588229]
EXISTENCE:mutant phenotype:GO:0097250 mitochondrial respirasome assembly [PMID:16135531]
Lyso-phosphatidylcholine acyltransferase; required for normal phospholipid content of mitochondrial membranes; major determinant of the final acyl chain composition of the mitochondrial-specific phospholipid cardiolipin; mutations in human ortholog tafazzin (TAZ) cause Barth syndrome, a rare X-linked disease characterized by skeletal and cardiomyopathy and bouts of cyclic neutropenia; a specific splice variant of human TAZ can complement yeast null mutant
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:14562095]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762|PMID:14562095]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:11729143|PMID:2138512|PMID:8106549]
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:14562095]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:2138512]
EXISTENCE:direct assay:GO:0008569 minus-end-directed microtubule motor activity [PMID:8041770]
EXISTENCE:direct assay:GO:0051321 meiotic cell cycle [PMID:11729143]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:9201713]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:2138512]
EXISTENCE:mutant phenotype:GO:0000743 nuclear migration involved in conjugation with cellular fusion [PMID:16380440|PMID:19386762|PMID:2138512]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:11729143]
EXISTENCE:mutant phenotype:GO:0060236 regulation of mitotic spindle organization [PMID:25313961]
Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:15769876]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15769876]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15769876]
Nucleolar protein; constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
ARS1627; Autonomously Replicating Sequence
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:22932476]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12446671]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:12446671]
EXISTENCE:physical interaction:GO:0030692 Noc4p-Nop14p complex [PMID:12446671]
Nucleolar protein; forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:28542199]
EXISTENCE:mutant phenotype:GO:1990882 rRNA acetylation [PMID:28542199]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:11081632]
C/D box small nucleolar RNA (snoRNA); acts as antisense guide to target RNA base acetylation by Kre33p to cytosine 1773 in 18S rRNA (responsible for ac4C 1773); establishes extended bipartite complementarity around the cytosines targeted for acetylation, similar to pseudouridylation pocket formation by the H/ACA snoRNPs; base pairing between snR45 and 18S rRNA requires the putative helicase activity of Kre33p
asparagine synthase (glutamine-hydrolyzing) 1
EXISTENCE:direct assay:GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity [PMID:6105958]
EXISTENCE:genetic interaction:GO:0006529 asparagine biosynthetic process [PMID:8951815]
Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
bifunctional triacylglycerol lipase/ester hydrolase
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:29491250]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
EXISTENCE:direct assay:GO:0016788 hydrolase activity, acting on ester bonds [PMID:29491250]
EXISTENCE:mutant phenotype:GO:0019433 triglyceride catabolic process [PMID:29491250]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:29491250]
Bifunctional triacylglycerol lipase and short chain ester hydrolase; null mutant results in the accumulation of both triacylglycerol and fatty acids derived from neutral lipids and phospholipids as well as an increase in the quantity of lipid droplets; contains an alpha/beta hydrolase domain with a conserved GXSXG lipase motif; localizes to lipid droplets; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
hypothetical protein; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:16622836]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:19064668]
EXISTENCE:direct assay:GO:0070250 mating projection membrane [PMID:19064668]
EXISTENCE:genetic interaction:GO:0061091 regulation of phospholipid translocation [PMID:27033517]
EXISTENCE:mutant phenotype:GO:0001933 negative regulation of protein phosphorylation [PMID:19564405]
EXISTENCE:mutant phenotype:GO:0007009 plasma membrane organization [PMID:19564405]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:8655575]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:27033517]
EXISTENCE:mutant phenotype:GO:0070941 eisosome assembly [PMID:19564405]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:20581291]
EXISTENCE:mutant phenotype:GO:0097576 vacuole fusion [PMID:33114062]
Protein involved in regulation of pheromone response and mating; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:15123691]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:15123691]
Protein required for degradation of unstable forms of cytochrome c; located in the mitochondria
EXISTENCE:direct assay:GO:0071010 prespliceosome [PMID:19941820]
hypothetical protein containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:31797992]
EXISTENCE:mutant phenotype:GO:0071464 cellular response to hydrostatic pressure [PMID:31797992]
Peripheral ER membrane protein; putative ER chaperone required for the stability and function of nutrient permeases exposed to high pressure
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23577202|PMID:14562095]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:23577202]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:23577202]
EXISTENCE:physical interaction:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:23577202]
Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:7723016]
EXISTENCE:mutant phenotype:GO:0016071 mRNA metabolic process [PMID:7723016]
Protein that regulates expression of Fo-F1 ATP synthase subunits; involved in the regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12562762]
EXISTENCE:mutant phenotype:GO:0000296 spermine transport [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000297 spermine transmembrane transporter activity [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11171066]
Polyamine transporter of major facilitator superfamily; member of 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to plasma membrane; targeted to vacuole via AP-3 pathway; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
hypothetical protein; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; TDA6 has a paralog, VPS62, that arose from the whole genome duplication
ARS1628; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18833194|PMID:22718905]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0034504 protein localization to nucleus [PMID:22718905]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:physical interaction:GO:0006886 intracellular protein transport [PMID:22718905]
EXISTENCE:physical interaction:GO:0034504 protein localization to nucleus [PMID:22718905]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:22718905]
Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
YPRWTy1-3; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
ARS1629; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
YPRCTy1-4; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
beta-glucan synthesis-associated protein KRE6
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17893149]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21193403]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:7929594]
EXISTENCE:direct assay:GO:0030133 transport vesicle [PMID:21193403]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:21193403]
EXISTENCE:mutant phenotype:GO:0006078 (1->6)-beta-D-glucan biosynthetic process [PMID:7929594]
EXISTENCE:mutant phenotype:GO:0015926 glucosidase activity [PMID:10601196]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7929594]
Glucosyl hydrolase required for beta-1,6-glucan biosynthesis; type II integral membrane protein that localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24146988|PMID:14562095]
EXISTENCE:direct assay:GO:0008184 glycogen phosphorylase activity [PMID:1613787]
EXISTENCE:mutant phenotype:GO:0005980 glycogen catabolic process [PMID:2657401]
EXISTENCE:mutant phenotype:GO:0008184 glycogen phosphorylase activity [PMID:2657401]
Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; phosphorylation by Cdc28p may coordinately regulate carbohydrate metabolism and the cell cycle; expression is regulated by stress-response elements and by the HOG MAP kinase pathway
cyclin-dependent serine/threonine protein kinase SGV1
EXISTENCE:direct assay:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:10982824]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14749387]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:10982824]
EXISTENCE:direct assay:GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity [PMID:11390638]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:11390638]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:12972617]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:10982824]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:12972617]
EXISTENCE:mutant phenotype:GO:0051571 positive regulation of histone H3-K4 methylation [PMID:16040246]
EXISTENCE:mutant phenotype:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19328068]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:10982824]
EXISTENCE:physical interaction:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:11390638]
Cyclin (Bur2p)-dependent protein kinase; subunit of the CDK-cyclin BUR kinase complex with Bur2p; regulates Set2p-dependent H3K36 trimethylation; regulates transcriptional elongation through the phosphorylation of the carboxy-terminal domain (CTD) of Rpo21p; stimulates recruitment of the PAF complex through Spt5p CTD phosphorylation; recruits Spt6p to the CTD at the onset of transcription; regulates telomere elongation; regulated by Cak1p; similar to metazoan CDK9 proteins
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16716188]
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:7543843]
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:23236192]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:7543843]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:24080224]
EXISTENCE:direct assay:GO:0033592 RNA strand annealing activity [PMID:7543843]
EXISTENCE:direct assay:GO:0034057 RNA strand-exchange activity [PMID:9769100]
EXISTENCE:direct assay:GO:0043024 ribosomal small subunit binding [PMID:23236192]
EXISTENCE:direct assay:GO:1902280 regulation of RNA helicase activity [PMID:24080224]
EXISTENCE:genetic interaction:GO:0006413 translational initiation [PMID:8404866]
EXISTENCE:genetic interaction:GO:0097010 eukaryotic translation initiation factor 4F complex assembly [PMID:23184954]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:23236192]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:8404865]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:8404866]
EXISTENCE:mutant phenotype:GO:0097010 eukaryotic translation initiation factor 4F complex assembly [PMID:23184954]
Translation initiation factor eIF-4B; RNA recognition motif containing single-stranded RNA binding protein that possesses RNA annealing and strand-exchange activities; stimulates the ATPase and unwinding activities of the RNA helicase eIF4A, in a synergistic manner with eIF4G; promotes assembly of the translation initiation factor complex eIF4F and the 48S preinitiation complex; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel
EXISTENCE:direct assay:GO:0035361 Cul8-RING ubiquitin ligase complex [PMID:20139071]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:18321796]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:0031297 replication fork processing [PMID:18704118]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19390089]
Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8195291]
EXISTENCE:direct assay:GO:0000148 1,3-beta-D-glucan synthase complex [PMID:8602515]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11598008]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11591390|PMID:7962098]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:15596542]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:1918143]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:8195291]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:8195291]
EXISTENCE:direct assay:GO:0007264 small GTPase mediated signal transduction [PMID:8621575]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12660244]
EXISTENCE:direct assay:GO:0090037 positive regulation of protein kinase C signaling [PMID:8621575]
EXISTENCE:direct assay:GO:0090334 regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [PMID:8602515|PMID:8662910]
EXISTENCE:genetic interaction:GO:0007117 budding cell bud growth [PMID:8195291]
EXISTENCE:genetic interaction:GO:0007264 small GTPase mediated signal transduction [PMID:8846785]
EXISTENCE:genetic interaction:GO:0031532 actin cytoskeleton reorganization [PMID:8947028]
EXISTENCE:genetic interaction:GO:0090037 positive regulation of protein kinase C signaling [PMID:8846785]
EXISTENCE:genetic interaction:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:12419188]
EXISTENCE:mutant phenotype:GO:0007117 budding cell bud growth [PMID:8195291]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:17302939]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:15596542]
EXISTENCE:mutant phenotype:GO:0032186 cellular bud neck septin ring organization [PMID:26179915]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:10893184]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:11598008]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:14593073]
EXISTENCE:mutant phenotype:GO:0060178 regulation of exocyst localization [PMID:11283608]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:8621575]
EXISTENCE:mutant phenotype:GO:0090037 positive regulation of protein kinase C signaling [PMID:8621575]
EXISTENCE:mutant phenotype:GO:0090334 regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [PMID:8602514|PMID:8602515|PMID:8662910]
EXISTENCE:mutant phenotype:GO:1903395 regulation of secondary cell septum biogenesis [PMID:23878277]
EXISTENCE:mutant phenotype:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:12419188]
EXISTENCE:physical interaction:GO:0031532 actin cytoskeleton reorganization [PMID:8947028]
EXISTENCE:physical interaction:GO:0031681 G-protein beta-subunit binding [PMID:12660244]
EXISTENCE:physical interaction:GO:0090037 positive regulation of protein kinase C signaling [PMID:8846785]
GTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
mitochondrial 37S ribosomal protein MRP2
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
phosphoadenylyl-sulfate reductase (thioredoxin)
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:3060034]
EXISTENCE:direct assay:GO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity [PMID:3060034]
EXISTENCE:direct assay:GO:0019379 sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) [PMID:3060034]
EXISTENCE:mutant phenotype:GO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity [PMID:2203779]
EXISTENCE:mutant phenotype:GO:0019379 sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) [PMID:197388]
3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9671481]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:27688401]
Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095|PMID:14690591]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:19806183]
Protein required for biogenesis of the large ribosomal subunit; required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein; essential gene
ARS1630; Autonomously Replicating Sequence
Putative, non-essential component vacuolar ATPase; conserved in fungi; SWAT-GFP fusion protein localizes to vacuole membrane; partially overlaps the dubious genes YPR169W-A, YPR170W-A and YRP170C
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12606027]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12606027]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12606027]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:12606027]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:12606027]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:12606027]
Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p
hypothetical protein; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:9606181]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:9606181]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:16601096]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:16505166|PMID:9155008|PMID:9606181]
EXISTENCE:direct assay:GO:0061709 reticulophagy [PMID:31802527]
EXISTENCE:genetic interaction:GO:0016125 sterol metabolic process [PMID:16096648]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:9606181]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:16096648]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:17428789]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:9155008]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:16505166]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:9211789|PMID:9606181]
EXISTENCE:mutant phenotype:GO:0070676 intralumenal vesicle formation [PMID:19234443]
AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15282802]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:15282802]
EXISTENCE:direct assay:GO:1902365 positive regulation of protein localization to spindle pole body [PMID:32553169]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
DNA polymerase epsilon noncatalytic subunit
EXISTENCE:curator inference:GO:0070868 heterochromatin organization involved in chromatin silencing [PMID:14673157]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:14622139|PMID:16916794]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:14622139]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:12882968]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15282802]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:12882968|PMID:14622139|PMID:16369485]
EXISTENCE:direct assay:GO:0008622 epsilon DNA polymerase complex [PMID:12571237|PMID:14673157|PMID:2406268]
EXISTENCE:direct assay:GO:0030337 DNA polymerase processivity factor activity [PMID:16369485]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:19776424]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:19776424]
EXISTENCE:mutant phenotype:GO:0045005 DNA-templated DNA replication maintenance of fidelity [PMID:18245343|PMID:19463834]
Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate
EXISTENCE:direct assay:GO:0004663 Rab geranylgeranyltransferase activity [PMID:8756702]
EXISTENCE:direct assay:GO:0005968 Rab-protein geranylgeranyltransferase complex [PMID:8183917|PMID:8583924|PMID:8756702]
EXISTENCE:direct assay:GO:0018344 protein geranylgeranylation [PMID:8756702]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:1903184]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:3312234|PMID:8232541]
EXISTENCE:physical interaction:GO:0005968 Rab-protein geranylgeranyltransferase complex [PMID:8232542]
Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p; human homolog RABGGTB can complement yeast BET2 mutant
EXISTENCE:mutant phenotype:GO:0000393 spliceosomal conformational changes to generate catalytic conformation [PMID:28864812]
EXISTENCE:mutant phenotype:GO:0034247 snoRNA splicing [PMID:8202378]
Splicing factor; component of the U4/U6-U5 snRNP complex
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:19573535]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16415367]
EXISTENCE:direct assay:GO:0004407 histone deacetylase activity [PMID:19573535]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0070823 HDA1 complex [PMID:11287668|PMID:8663039]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11287668]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16415367]
EXISTENCE:mutant phenotype:GO:0031047 RNA-mediated gene silencing [PMID:18022365]
EXISTENCE:physical interaction:GO:0070823 HDA1 complex [PMID:11287668]
Subunit of HDA1 histone deacetylase complex; tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for activity of complex; relocalizes to cytosol in response to hypoxia; similar to Hda2p; the HDA1 histone deacetylase complex regulates aging through trehalose metabolism
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:9312010]
EXISTENCE:direct assay:GO:0019948 SUMO activating enzyme activity [PMID:9312010]
EXISTENCE:physical interaction:GO:0031510 SUMO activating enzyme complex [PMID:9312010]
Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0003400 regulation of COPII vesicle coating [PMID:8451644]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:8451644]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:31273116]
GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
EXISTENCE:curator inference:GO:0000398 mRNA splicing, via spliceosome []
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:9012791]
EXISTENCE:direct assay:GO:0008266 poly(U) RNA binding [PMID:12618433]
EXISTENCE:physical interaction:GO:1990935 splicing factor binding [PMID:9528767]
Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
dolichyl-phosphate beta-D-mannosyltransferase
EXISTENCE:direct assay:GO:0004582 dolichyl-phosphate beta-D-mannosyltransferase activity [PMID:16549409|PMID:3053713]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:1803815|PMID:26928762]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:10419517]
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:2146492]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:2146492]
Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane; catalyzes formation of Dol-P-Man from Dol-P and GDP-Man; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation; human homolog DPM1 can complement yeast mutant strains
bifunctional 4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase
EXISTENCE:direct assay:GO:0004134 4-alpha-glucanotransferase activity [PMID:11094287]
EXISTENCE:direct assay:GO:0004135 amylo-alpha-1,6-glucosidase activity [PMID:11094287]
EXISTENCE:mutant phenotype:GO:0004134 4-alpha-glucanotransferase activity [PMID:11094287]
EXISTENCE:mutant phenotype:GO:0004135 amylo-alpha-1,6-glucosidase activity [PMID:11094287]
EXISTENCE:mutant phenotype:GO:0005980 glycogen catabolic process [PMID:11094287]
Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
serine/threonine protein kinase regulatory subunit ATG13
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:29540529]
EXISTENCE:direct assay:GO:0019887 protein kinase regulator activity [PMID:22885598]
EXISTENCE:direct assay:GO:0070016 armadillo repeat domain binding [PMID:31512555]
EXISTENCE:genetic interaction:GO:0034497 protein localization to phagophore assembly site [PMID:23509291]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:32508216]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:9224892]
EXISTENCE:mutant phenotype:GO:0019887 protein kinase regulator activity [PMID:10995454]
EXISTENCE:mutant phenotype:GO:0032147 activation of protein kinase activity [PMID:10995454]
EXISTENCE:mutant phenotype:GO:0071211 protein targeting to vacuole involved in autophagy [PMID:27768871]
EXISTENCE:mutant phenotype:GO:0071255 Cvt vesicle assembly [PMID:10837477]
EXISTENCE:physical interaction:GO:1990316 Atg1/ULK1 kinase complex [PMID:15743910]
Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:2642897]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2642897]
EXISTENCE:direct assay:GO:0001010 RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity [PMID:8496187]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2642897]
EXISTENCE:mutant phenotype:GO:0001010 RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity [PMID:8496187]
Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
DNA-directed RNA polymerase core subunit RPO26
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:12697831]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:12697831]
RNA polymerase subunit ABC23; common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15210731]
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:15210731]
EXISTENCE:genetic interaction:GO:1990274 mitotic actomyosin contractile ring disassembly [PMID:19109423]
EXISTENCE:mutant phenotype:GO:1990274 mitotic actomyosin contractile ring disassembly [PMID:15210731]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15210731]
EXISTENCE:physical interaction:GO:0016460 myosin II complex [PMID:15210731]
EXISTENCE:physical interaction:GO:0032038 myosin II heavy chain binding [PMID:15210731]
Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
SKI complex subunit tetratricopeptide repeat protein SKI3
EXISTENCE:direct assay:GO:0055087 Ski complex [PMID:10744028|PMID:18042677]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:10611222|PMID:9482746]
EXISTENCE:mutant phenotype:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:11910110|PMID:17660569]
EXISTENCE:physical interaction:GO:0055087 Ski complex [PMID:18042677]
Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
DNA-directed RNA polymerase III subunit C82
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:1406632]
ubiquinol--cytochrome-c reductase subunit 2
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:direct assay:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:3028797]
EXISTENCE:direct assay:GO:0030061 mitochondrial crista [PMID:19019989]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:3028797]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:3028797]
Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10092523]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:9765289]
EXISTENCE:genetic interaction:GO:0015250 water channel activity [PMID:11158584]
EXISTENCE:genetic interaction:GO:0055085 transmembrane transport [PMID:11158584]
EXISTENCE:mutant phenotype:GO:0006833 water transport [PMID:9765289]
EXISTENCE:mutant phenotype:GO:0015250 water channel activity [PMID:9765289]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:15583134]
Spore-specific water channel; mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance
EXISTENCE:direct assay:GO:0006473 protein acetylation [PMID:23775086]
EXISTENCE:direct assay:GO:0008080 N-acetyltransferase activity [PMID:23775086]
Tetrameric histone acetyltransferase; has similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; also acetylates polyamines
EXISTENCE:curator inference:GO:0005886 plasma membrane [PMID:9643541]
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:25359886]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:25130273]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25359886]
EXISTENCE:direct assay:GO:0006857 oligopeptide transport [PMID:9643541]
EXISTENCE:direct assay:GO:0035673 oligopeptide transmembrane transporter activity [PMID:9643541]
EXISTENCE:genetic interaction:GO:0044088 regulation of vacuole organization [PMID:25359886]
EXISTENCE:genetic interaction:GO:0061091 regulation of phospholipid translocation [PMID:25359886]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:19767828]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:25130273]
EXISTENCE:mutant phenotype:GO:0051515 positive regulation of monopolar cell growth [PMID:25359886]
Oligopeptide transporter; localized to peroxisomes and affects glutathione redox homeostasis; also localizes to the plasma membrane (PM) and to the late Golgi, and has a role in maintenance of lipid asymmetry between the inner and outer leaflets of the PM; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles and in polarized cell growth
putative maltose-responsive transcription factor
ARS1631; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9483795]
EXISTENCE:mutant phenotype:GO:0042910 xenobiotic transmembrane transporter activity [PMID:9483795]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9483795]
Plasma membrane multidrug transporter; member of the major facilitator superfamily; acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:18593383]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:18593383]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:24358276]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14978214|PMID:15147884]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15147884]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11527213|PMID:14978214]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:18593383]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11527213|PMID:14978214|PMID:15147884|PMID:15575969|P MID:18593383]
Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds; directly binds trivalent arsenic (As(III)) as does K. lactis ortholog, KIYAP8
EXISTENCE:direct assay:GO:0030611 arsenate reductase activity [PMID:10801893]
EXISTENCE:mutant phenotype:GO:0030611 arsenate reductase activity [PMID:10801893]
Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20655873]
EXISTENCE:direct assay:GO:0015105 arsenite transmembrane transporter activity [PMID:24291645]
EXISTENCE:mutant phenotype:GO:0015104 antimonite transmembrane transporter activity [PMID:20655873]
EXISTENCE:mutant phenotype:GO:0015105 arsenite transmembrane transporter activity [PMID:24291645]
EXISTENCE:mutant phenotype:GO:0015297 antiporter activity [PMID:21447319]
EXISTENCE:mutant phenotype:GO:0015699 antimonite transport [PMID:20655873]
EXISTENCE:mutant phenotype:GO:0015700 arsenite transport [PMID:24291645]
Plasma membrane metalloid/H+ antiporter; member of bile/arsenite/riboflavin transporter (BART) superfamily; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite; protein displays 10 transmembrane segments with cytoplasmically oriented N- and C-terminal domains
ARS1632; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
TEL16R; Telomeric region on the right arm of Chromosome XVI; annotated components include an X element core sequence and a short Y' element; TEL16R does have telomeric repeats (TEL16R-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
hypothetical protein; similar to telomere-encoded helicases; down-regulated at low calcium levels; YPR202W is not an essential gene; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9837911]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:9837911]
DNA helicase encoded within the telomeric Y' element; Y' -helicase protein 1