Saccharomyces cerevisiae S288C chromosome XV, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome XV
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (27-MAY-2010) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006948.\n\nOn Apr 26, 2011 this sequence version replaced NC_001147.5.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##
TEL15L; Telomeric region on the left arm of Chromosome XV; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD15 has a paralog, AAD3, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:mutant phenotype:GO:0018741 alkyl sulfatase activity [PMID:15947202]
EXISTENCE:mutant phenotype:GO:0018909 dodecyl sulfate metabolic process [PMID:15947202]
Bacterially-derived sulfatase; required for use of alkyl sulfates as sulfur source
hypothetical protein; proposed to be an inactive evolutionary remnant that along with neighboring gene YOL162W may have constituted a second sulfonate transporter and SOA1 paralog that is intact in S. uvarum, S. eubayanus and S. arboricola and named SOA2; member of the Dal5p subfamily of the major facilitator family
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
hypothetical protein; proposed to be an inactive evolutionary remnant that along with neighboring gene YOL163W may have constituted a second sulfonate transporter and SOA1 paralog that is intact in S. uvarum, S. eubayanus and S. arboricola and named SOA2; member of the Dal5p subfamily of the major facilitator family
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole; expression induced by low temperature and also by anaerobic conditions; induced during alcoholic fermentation
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21777356]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21777356|PMID:14562095]
hypothetical protein; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:26928762]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
hypothetical protein, secreted when constitutively expressed; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the cell periphery, SWAT-GFP fusion also localizes to the extracellular region, and mCherry fusion also localizes to the vacuole; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17714436]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:17714436]
EXISTENCE:mutant phenotype:GO:0015620 ferric-enterobactin transmembrane transporter activity [PMID:10831226]
EXISTENCE:mutant phenotype:GO:0015685 ferric-enterobactin import into cell [PMID:10831226]
Ferric enterobactin transmembrane transporter; expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
EXISTENCE:direct assay:GO:0004574 oligo-1,6-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0004575 sucrose alpha-glucosidase activity [PMID:24649402]
EXISTENCE:genetic interaction:GO:0046352 disaccharide catabolic process [PMID:20562106|PMID:20471265]
EXISTENCE:mutant phenotype:GO:0004574 oligo-1,6-glucosidase activity [PMID:20562106]
Isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; not required for isomaltose utilization, but Ima2p overexpression allows the ima1 null mutant to grow on isomaltose
EXISTENCE:genetic interaction:GO:1902341 xylitol transmembrane transport [PMID:26996892]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005354 galactose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Hexose transporter; capable of transporting a broad range of substrates including: glucose, fructose, mannose and galactose; polyol transporter that supports the growth on and uptake of xylitol with low affinity when overexpressed in a strain deleted for hexose family members; nearly identical in sequence to Hxt9p; has similarity to major facilitator superfamily (MFS) transporters; involved in pleiotropic drug resistance
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11935221]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11748726]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:11748726]
Haze-protective mannoprotein; reduces particle size of aggregated proteins in white wines, thereby decreasing turbidity; intragenic repeat expansion controls chronological aging; expansion of intragenic tandem repeats within N-terminus sufficient to cause pronounced life span shortening; repeat expansion shifts cells from sedentary to buoyant, thereby increasing exposure to oxygen, altering methionine, lipid, and purine metabolism
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11935221]
Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH
ARS1531; Autonomously Replicating Sequence
Blocked reading frame, contains two in-frame stops in translation; this ORF is conserved, without the stops, in Saccharomyces paradoxus, S. mikatae, and S. bayanus; RNA sequencing data indicate the presence of a transcription unit at this reading frame
EXISTENCE:direct assay:GO:0000293 ferric-chelate reductase activity [PMID:17681937]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17553781]
EXISTENCE:genetic interaction:GO:0000293 ferric-chelate reductase activity [PMID:17553781]
EXISTENCE:genetic interaction:GO:0006826 iron ion transport [PMID:17553781]
EXISTENCE:genetic interaction:GO:0015677 copper ion import [PMID:17553781]
Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels
methylglyoxal reductase (NADPH-dependent) GRE2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12206772]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12206772]
EXISTENCE:direct assay:GO:0043892 methylglyoxal reductase (NADPH-dependent) activity [PMID:12722185]
EXISTENCE:genetic interaction:GO:0008204 ergosterol metabolic process [PMID:16598690]
EXISTENCE:mutant phenotype:GO:0008204 ergosterol metabolic process [PMID:16598690]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:16999827]
EXISTENCE:mutant phenotype:GO:0046568 3-methylbutanol:NAD(P) oxidoreductase activity [PMID:16999827]
3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:11139489]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23509072|PMID:12730603]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11139489]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23706738|PMID:22842922]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:16341225]
EXISTENCE:direct assay:GO:0098562 cytoplasmic side of membrane [PMID:27146487]
EXISTENCE:direct assay:GO:0098745 Dcp1-Dcp2 complex [PMID:12554866]
EXISTENCE:mutant phenotype:GO:0000932 P-body [PMID:12730603]
EXISTENCE:physical interaction:GO:0098745 Dcp1-Dcp2 complex [PMID:10508173]
Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9224714|PMID:9674426]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:mutant phenotype:GO:0003712 transcription coregulator activity [PMID:8649430]
EXISTENCE:mutant phenotype:GO:0036498 IRE1-mediated unfolded protein response [PMID:10652329]
Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:7860627|PMID:7721939]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20028986]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831]
EXISTENCE:mutant phenotype:GO:0016559 peroxisome fission [PMID:7721939|PMID:7860627]
EXISTENCE:mutant phenotype:GO:0019395 fatty acid oxidation [PMID:10931862]
Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:genetic interaction:GO:0006261 DNA-templated DNA replication [PMID:12730134]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:physical interaction:GO:0000811 GINS complex [PMID:12768207|PMID:12730134]
EXISTENCE:physical interaction:GO:0031261 DNA replication preinitiation complex [PMID:12730134]
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:15531585]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10219085|PMID:10684247|PMID:15643076]
EXISTENCE:direct assay:GO:0045142 triplex DNA binding [PMID:11058104]
EXISTENCE:direct assay:GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding [PMID:22796944]
EXISTENCE:direct assay:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006360 transcription by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10219085]
EXISTENCE:mutant phenotype:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23109428]
EXISTENCE:mutant phenotype:GO:0006362 transcription elongation by RNA polymerase I [PMID:19164765]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15149594]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0060260 regulation of transcription initiation by RNA polymerase II [PMID:18194564]
EXISTENCE:mutant phenotype:GO:1901525 negative regulation of mitophagy [PMID:31525119]
EXISTENCE:mutant phenotype:GO:2001165 positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:18469135]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:11884586]
EXISTENCE:physical interaction:GO:0003712 transcription coregulator activity [PMID:11927560]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:10219085|PMID:11884586|PMID:11927560]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11927560]
Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9891085]
EXISTENCE:direct assay:GO:0008428 ribonuclease inhibitor activity [PMID:21747919]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9891085]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:9891085]
Nucleolar protein required for 60S ribosomal subunit biogenesis
EXISTENCE:direct assay:GO:0000906 6,7-dimethyl-8-ribityllumazine synthase activity [PMID:7559556]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:5366000|PMID:7559556]
Lumazine synthase (DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin; DMRL synthase stands for 6,7-dimethyl-8-ribityllumazine synthase
exosome non-catalytic core subunit RRP40
EXISTENCE:curator inference:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:10465791]
EXISTENCE:curator inference:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10465791]
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:10465791]
EXISTENCE:curator inference:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:19046973|PMID:10465791]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:17391830]
EXISTENCE:direct assay:GO:0030145 manganese ion binding [PMID:17159918]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain both S1 and KH RNA binding domains; mutations in the human homolog, EXOSC3, cause pontocerebellar hypoplasia with motor neuron degeneration
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0008175 tRNA methyltransferase activity [PMID:16642040]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0008175 tRNA methyltransferase activity [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0031591 wybutosine biosynthetic process [PMID:17150819|PMID:16642040]
AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
ETC2; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; remains predominantly localized to the nuclear periphery throughout cell cycle; located between PPM2 and ARG8
EXISTENCE:direct assay:GO:0003992 N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity [PMID:2199330]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:205532]
Acetylornithine aminotransferase; catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
EXISTENCE:direct assay:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:15753296]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8119957]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8119957]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:17908917|PMID:18981231|PMID:26777405]
EXISTENCE:direct assay:GO:0016281 eukaryotic translation initiation factor 4F complex [PMID:15753296]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:16343487]
EXISTENCE:direct assay:GO:0098808 mRNA cap binding [PMID:24183571]
EXISTENCE:direct assay:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:30281017]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:2540596]
Translation initiation factor eIF4E; mRNA cap binding protein and subunit of the mRNA cap-binding complex (eIF4F) along with eIF4A (Tif1p/Tif2p) and eIF4G (Tif4631p/Tif4632p), that mediates cap-dependent translation; required with eIF4G for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; protein abundance increases in response to DNA replication stress; human homolog EIF4E can complement the cdc33 null mutant
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:23716719]
Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
hypothetical protein with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:8861205]
EXISTENCE:mutant phenotype:GO:0006003 fructose 2,6-bisphosphate metabolic process [PMID:8861205]
EXISTENCE:mutant phenotype:GO:0006110 regulation of glycolytic process [PMID:8861205]
6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:19057509]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16452140]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
SCF ubiquitin ligase complex subunit HRT1
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:10385629]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11080155]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10385629]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:10385629|PMID:19882662]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:10213692]
EXISTENCE:direct assay:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:10385629]
EXISTENCE:direct assay:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:12676951]
EXISTENCE:direct assay:GO:0035361 Cul8-RING ubiquitin ligase complex [PMID:12676951]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:10230407]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10385629]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:10385629]
RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
EXISTENCE:direct assay:GO:0042124 1,3-beta-glucanosyltransferase activity [PMID:17397106]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:17189486]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:17189486]
1,3-beta-glucanosyltransferase; involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall
ARS1507; Autonomously Replicating Sequence
hypothetical protein; YOL131W has a paralog, STB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21738593]
EXISTENCE:direct assay:GO:0015693 magnesium ion transport [PMID:12167543]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:9430719]
EXISTENCE:mutant phenotype:GO:0006812 cation transport [PMID:9430719]
EXISTENCE:mutant phenotype:GO:0022890 inorganic cation transmembrane transporter activity [PMID:9430719]
Plasma membrane Mg(2+) transporter; expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; magnesium transport defect of the null mutant is functionally complemented by either of the human genes MAGT1 and TUSC3 that are not orthologous to ALR1
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:14562095]
EXISTENCE:direct assay:GO:0034424 Vps55/Vps68 complex [PMID:18216282]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:18216282]
EXISTENCE:physical interaction:GO:0034424 Vps55/Vps68 complex [PMID:18216282]
Vacuolar membrane hypothetical protein; involved in vacuolar protein sorting; also detected in the mitochondria
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:10958669]
EXISTENCE:mutant phenotype:GO:0006508 proteolysis [PMID:10958669]
Protein kinase related to mammalian GSK-3 glycogen synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation; YGK3 has a paralog, MCK1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:8028007]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23268442]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:8028007]
Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:1986231]
EXISTENCE:direct assay:GO:0030060 L-malate dehydrogenase activity [PMID:1986231]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:12730240]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:19118449]
EXISTENCE:mutant phenotype:GO:0030060 L-malate dehydrogenase activity [PMID:1986231]
Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1; mutation in human homolog MDH2 causes early-onset severe encephalopathy
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:17242307]
EXISTENCE:direct assay:GO:0106050 tRNA 2'-O-methyltransferase activity [PMID:17242307]
EXISTENCE:mutant phenotype:GO:0002128 tRNA nucleoside ribose methylation [PMID:32053677]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:17242307]
EXISTENCE:mutant phenotype:GO:0106050 tRNA 2'-O-methyltransferase activity [PMID:17242307]
2'-O-methyltransferase; responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
ARS1508; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:15899842]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15899842|PMID:14562095]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:15899842]
EXISTENCE:mutant phenotype:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:15899842]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:15899842]
EXISTENCE:physical interaction:GO:0043528 tRNA (m2G10) methyltransferase complex [PMID:15899842]
Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain; another complex member is Trm112p
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9334319]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:15703440|PMID:23222640|PMID:17684230]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9334319|PMID:15572678|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9334319]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:26777405]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:9334319]
EXISTENCE:mutant phenotype:GO:0072423 response to DNA damage checkpoint signaling [PMID:24603480]
EXISTENCE:physical interaction:GO:0005849 mRNA cleavage factor complex [PMID:11344258]
Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability; plays a role in copper homeostasis
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8643535]
EXISTENCE:direct assay:GO:0015295 solute:proton symporter activity [PMID:10574989]
EXISTENCE:direct assay:GO:0022890 inorganic cation transmembrane transporter activity [PMID:10574989]
EXISTENCE:genetic interaction:GO:0006825 copper ion transport [PMID:9115231]
EXISTENCE:genetic interaction:GO:0006826 iron ion transport [PMID:10930410]
EXISTENCE:genetic interaction:GO:0006876 cellular cadmium ion homeostasis [PMID:9115231]
EXISTENCE:genetic interaction:GO:0006878 cellular copper ion homeostasis [PMID:9115231]
EXISTENCE:genetic interaction:GO:0015691 cadmium ion transport [PMID:9115231]
EXISTENCE:mutant phenotype:GO:0006828 manganese ion transport [PMID:8643535]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:8643535]
Divalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728|PMID:16159874]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:16159874]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:16159874]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:16159874]
Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11536335]
Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
EXISTENCE:direct assay:GO:0008180 COP9 signalosome [PMID:12446563]
EXISTENCE:genetic interaction:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12446563]
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:12186635|PMID:12446563]
Subunit of the COP9 signalosome (CSN) complex; this complex cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2263457|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:20802496]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10409737]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15773992|PMID:8710886|PMID:9987114|PMID:10409737|PMI D:8662191]
Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0004652 polynucleotide adenylyltransferase activity [PMID:12062100|PMID:15935758|PMID:15935759|PMID:16260630|P MID:16374505]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10066793|PMID:22932476]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16541108]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:15828860|PMID:15935758|PMID:15935759]
EXISTENCE:direct assay:GO:0034458 3'-5' RNA helicase activity [PMID:22532666]
EXISTENCE:direct assay:GO:0043629 ncRNA polyadenylation [PMID:15935758|PMID:15828860]
EXISTENCE:direct assay:GO:0051575 5'-deoxyribose-5-phosphate lyase activity [PMID:17983848]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:15935758|PMID:17643380]
EXISTENCE:genetic interaction:GO:0004652 polynucleotide adenylyltransferase activity [PMID:16374505]
EXISTENCE:genetic interaction:GO:0006284 base-excision repair [PMID:17983848]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:16373491]
EXISTENCE:genetic interaction:GO:0043629 ncRNA polyadenylation [PMID:15145828|PMID:15935759|PMID:16373491|PMID:16374505]
EXISTENCE:genetic interaction:GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing [PMID:17410208]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:16541108]
EXISTENCE:genetic interaction:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:15935758|PMID:16373491]
EXISTENCE:genetic interaction:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15145828]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:15935759]
EXISTENCE:genetic interaction:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:16373491]
EXISTENCE:genetic interaction:GO:0071044 histone mRNA catabolic process [PMID:17179095]
EXISTENCE:genetic interaction:GO:0071050 sno(s)RNA polyadenylation [PMID:18951092]
EXISTENCE:genetic interaction:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:16373491]
EXISTENCE:mutant phenotype:GO:0004652 polynucleotide adenylyltransferase activity [PMID:15828860|PMID:15935759|PMID:16260630]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:17983848]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:22319136]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:16373491]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:25210768]
EXISTENCE:mutant phenotype:GO:0043629 ncRNA polyadenylation [PMID:15828860|PMID:16431988]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:23762389]
EXISTENCE:mutant phenotype:GO:0051575 5'-deoxyribose-5-phosphate lyase activity [PMID:17983848]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:16431988|PMID:18007593]
EXISTENCE:mutant phenotype:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:15935758]
EXISTENCE:mutant phenotype:GO:0071037 nuclear polyadenylation-dependent snRNA catabolic process [PMID:15935758|PMID:16431988]
EXISTENCE:mutant phenotype:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:16431988|PMID:18456844]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258|PMID:15935759|PMID:16973436|PMID:18007593]
EXISTENCE:mutant phenotype:GO:0071040 nuclear polyadenylation-dependent antisense transcript catabolic process [PMID:18022365]
EXISTENCE:mutant phenotype:GO:0071047 polyadenylation-dependent mRNA catabolic process [PMID:19369424]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27076633]
Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:33686140]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:25683707]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:33686140]
Stalled-ribosome rescue factor srRF1; similar to human peptidyl-tRNA hydrolase ICT1; associates with mitochondrial large subunit; not an essential gene
putative serine/threonine protein kinase SKM1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19793923]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15023338]
EXISTENCE:genetic interaction:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:9044278]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:19793923]
Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10679030]
EXISTENCE:direct assay:GO:0000133 polarisome [PMID:16166638]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10937877]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10679030]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:35134079]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:12913108]
EXISTENCE:genetic interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:16166638]
EXISTENCE:physical interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:12913108|PMID:16166638]
GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16390866|PMID:23285234]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:20850366]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23285234|PMID:26777405]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:17441508]
EXISTENCE:direct assay:GO:0072380 TRC complex [PMID:20850366]
EXISTENCE:genetic interaction:GO:0045048 protein insertion into ER membrane [PMID:19325107]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:16390866]
Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:12193598]
EXISTENCE:direct assay:GO:0018345 protein palmitoylation [PMID:12193598]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:7532279]
EXISTENCE:mutant phenotype:GO:0018345 protein palmitoylation [PMID:16751107]
EXISTENCE:physical interaction:GO:0031211 endoplasmic reticulum palmitoyltransferase complex [PMID:12193598]
Palmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12949174|PMID:16622836]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:12949174]
Peripheral membrane protein of the plasma membrane; interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:8195172]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8195172]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15455074]
EXISTENCE:direct assay:GO:0090575 RNA polymerase II transcription regulator complex [PMID:8195172]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8195172]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:2834106]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1461729|PMID:8195172]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8195172]
EXISTENCE:physical interaction:GO:0090575 RNA polymerase II transcription regulator complex [PMID:8195172]
Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
ARS1509; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27337501]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:mutant phenotype:GO:0004888 transmembrane signaling receptor activity [PMID:10430578]
EXISTENCE:mutant phenotype:GO:0007266 Rho protein signal transduction [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20385774]
Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:25897084|PMID:9157883|PMID:9242487]
EXISTENCE:direct assay:GO:0007129 homologous chromosome pairing at meiosis [PMID:9157883]
EXISTENCE:direct assay:GO:0035974 meiotic spindle pole body [PMID:25897084]
EXISTENCE:direct assay:GO:0045141 meiotic telomere clustering [PMID:12734403]
EXISTENCE:genetic interaction:GO:0007129 homologous chromosome pairing at meiosis [PMID:17495028]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:17495028|PMID:9157883]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:16648465]
EXISTENCE:mutant phenotype:GO:0010520 regulation of reciprocal meiotic recombination [PMID:18818741|PMID:18818742]
EXISTENCE:mutant phenotype:GO:0045141 meiotic telomere clustering [PMID:11018056]
EXISTENCE:mutant phenotype:GO:0070197 meiotic attachment of telomere to nuclear envelope [PMID:18818741|PMID:18818742]
EXISTENCE:mutant phenotype:GO:2000711 positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric [PMID:25897084]
Protein that regulates meiotic SPB cohesion and telomere clustering; localizes to both spindle pole bodies (SPBs) and telomeres; required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
YOLWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16233524]
EXISTENCE:mutant phenotype:GO:0005365 myo-inositol transmembrane transporter activity [PMID:2040626]
EXISTENCE:mutant phenotype:GO:0015798 myo-inositol transport [PMID:2040626]
Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000215 tRNA 2'-phosphotransferase activity [PMID:9148937]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22391451]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22391451]
EXISTENCE:direct assay:GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation [PMID:9148937]
tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:15060275]
Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:10567559|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:22918958]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12221112]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:12221112]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:18034155]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:10567559]
EXISTENCE:genetic interaction:GO:0010606 positive regulation of cytoplasmic mRNA processing body assembly [PMID:21163942]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:22918958]
EXISTENCE:genetic interaction:GO:0060211 regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:21163942]
EXISTENCE:mutant phenotype:GO:0000196 cell wall integrity MAPK cascade [PMID:10567559]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:22918958]
EXISTENCE:mutant phenotype:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:12221112]
Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
Putative metalloprotease; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position C663
EXISTENCE:direct assay:GO:0004830 tryptophan-tRNA ligase activity [PMID:9046085]
Cytoplasmic tryptophanyl-tRNA synthetase; aminoacylates tryptophanyl-tRNA; human homolog WARS can complement yeast null mutant
hexaprenyldihydroxybenzoate methyltransferase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10760477|PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10419476]
EXISTENCE:direct assay:GO:0006744 ubiquinone biosynthetic process [PMID:10419476]
EXISTENCE:mutant phenotype:GO:0004395 hexaprenyldihydroxybenzoate methyltransferase activity [PMID:1885593]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:1885593]
EXISTENCE:mutant phenotype:GO:0061543 3-demethylubiquinol-6 3-O-methyltransferase activity [PMID:10419476]
O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10409639]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:16358299]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:10669756]
EXISTENCE:mutant phenotype:GO:0032042 mitochondrial DNA metabolic process [PMID:16358299]
Mitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:12604797|PMID:8995429]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005663 DNA replication factor C complex [PMID:8063832]
EXISTENCE:direct assay:GO:0006272 leading strand elongation [PMID:1346062]
EXISTENCE:direct assay:GO:0007062 sister chromatid cohesion [PMID:11389843]
EXISTENCE:direct assay:GO:0031389 Rad17 RFC-like complex [PMID:12604797]
EXISTENCE:physical interaction:GO:0031389 Rad17 RFC-like complex [PMID:10660302]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
EXISTENCE:physical interaction:GO:0031391 Elg1 RFC-like complex [PMID:12909721|PMID:12912927]
Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
tRNA (guanine(9)-N(1))-methyltransferase
EXISTENCE:direct assay:GO:0016423 tRNA (guanine) methyltransferase activity [PMID:12702816]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:12702816]
tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23169667|PMID:14562095|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:23169667]
EXISTENCE:mutant phenotype:GO:0034488 basic amino acid transmembrane export from vacuole [PMID:23169667]
Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:24390141]
EXISTENCE:direct assay:GO:0035974 meiotic spindle pole body [PMID:10899120]
EXISTENCE:genetic interaction:GO:0032120 ascospore-type prospore membrane formation [PMID:10899120]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:10899120]
Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation; SPO21 has a paralog, YSW1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:10882115]
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:10066781|PMID:9018043]
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:16781730]
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:16781730]
EXISTENCE:direct assay:GO:0000406 double-strand/single-strand DNA junction binding [PMID:16781730]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:9545323]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9819445]
EXISTENCE:direct assay:GO:0032135 DNA insertion or deletion binding [PMID:8805366]
EXISTENCE:direct assay:GO:0032137 guanine/thymine mispair binding [PMID:8816473]
EXISTENCE:direct assay:GO:0032138 single base insertion or deletion binding [PMID:8816473]
EXISTENCE:genetic interaction:GO:0000735 removal of nonhomologous ends [PMID:9256462]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16157874]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:1334021|PMID:16702432|PMID:8056309]
EXISTENCE:mutant phenotype:GO:0000735 removal of nonhomologous ends [PMID:10523644|PMID:9256462]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:10523644|PMID:9520271]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:15920474]
EXISTENCE:mutant phenotype:GO:0006311 meiotic gene conversion [PMID:1334021]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:8849883]
EXISTENCE:mutant phenotype:GO:0032138 single base insertion or deletion binding [PMID:9111312]
EXISTENCE:mutant phenotype:GO:0043111 replication fork arrest [PMID:27045900]
EXISTENCE:physical interaction:GO:0032301 MutSalpha complex [PMID:8816473]
EXISTENCE:physical interaction:GO:0032302 MutSbeta complex [PMID:8805366]
Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP; required for silencing at the silent mating-type loci and telomeres
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:11943786|PMID:9559673]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:9559673]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11943786|PMID:9559673]
Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003756 protein disulfide isomerase activity [PMID:16002399]
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:16002399]
EXISTENCE:genetic interaction:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
EXISTENCE:genetic interaction:GO:0005783 endoplasmic reticulum [PMID:15377672]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:11157982]
EXISTENCE:genetic interaction:GO:0015035 protein-disulfide reductase activity [PMID:11157982]
EXISTENCE:mutant phenotype:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
Member of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
Ubiquitin-binding hypothetical protein; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
EXISTENCE:direct assay:GO:0071821 FANCM-MHF complex [PMID:22325783]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:20347428]
Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:6985717]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:2937632]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:3546317]
EXISTENCE:direct assay:GO:0019170 methylglyoxal reductase (NADH-dependent) activity [PMID:12722185]
EXISTENCE:direct assay:GO:0045069 regulation of viral genome replication [PMID:31648290]
EXISTENCE:direct assay:GO:0046809 replication compartment [PMID:31648290]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:genetic interaction:GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0006116 NADH oxidation [PMID:15082781|PMID:23744286]
EXISTENCE:mutant phenotype:GO:0019655 glycolytic fermentation to ethanol [PMID:15082781]
Alcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YOL085C is not an essential gene; partially overlaps dubious ORF YOL085W-A
hypothetical protein; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0034270 Cvt complex [PMID:20639194|PMID:20659891]
EXISTENCE:genetic interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:20639194]
EXISTENCE:genetic interaction:GO:0031503 protein-containing complex localization [PMID:20639194|PMID:20659891]
EXISTENCE:genetic interaction:GO:0071211 protein targeting to vacuole involved in autophagy [PMID:20639194]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:20639194]
Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
ARS1510; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:12479808|PMID:18497569]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11382752]
EXISTENCE:direct assay:GO:0034270 Cvt complex [PMID:11430817|PMID:12479808|PMID:20659891]
EXISTENCE:genetic interaction:GO:0031503 protein-containing complex localization [PMID:20659891]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:15138258]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:17238920]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:12479808]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:11430817|PMID:12479808|PMID:12479807|PMID:20659891]
EXISTENCE:mutant phenotype:GO:0061912 selective autophagy [PMID:29673596]
Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:7577970]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22575457|PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:22575457]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:2178777]
EXISTENCE:mutant phenotype:GO:0007193 adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway [PMID:15629464|PMID:2164637]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15629464]
EXISTENCE:mutant phenotype:GO:0043547 positive regulation of GTPase activity [PMID:2178777]
EXISTENCE:mutant phenotype:GO:0046580 negative regulation of Ras protein signal transduction [PMID:15339905]
EXISTENCE:mutant phenotype:GO:0070301 cellular response to hydrogen peroxide [PMID:15629464]
EXISTENCE:mutant phenotype:GO:0070417 cellular response to cold [PMID:15629464]
EXISTENCE:mutant phenotype:GO:0071248 cellular response to metal ion [PMID:15629464]
GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:genetic interaction:GO:0000027 ribosomal large subunit assembly [PMID:12364601]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:12364601]
Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:19144819]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19144819]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:16002396]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:12408816]
EXISTENCE:physical interaction:GO:0005737 cytoplasm [PMID:12408816]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:physical interaction:GO:0031932 TORC2 complex [PMID:12408816]
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; C-terminal PH domain binds to phosphoinositide-(4,5)-bisphosphate (PI(4,5)P2); binds directly to Ypk2p a Tor2p substrate and downstream TORC2 effector through a conserved central CRIM domain; acts with Tsc11p as a scaffold important for the integrity of the TORC2 complex; dispensable for PM localization of TORC2; role in regulating actin organization and growth control; orthologous to human MAPKAP1/SIN1
EXISTENCE:curator inference:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:20219971]
Subunit k of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11843177]
EXISTENCE:direct assay:GO:0008097 5S rRNA binding [PMID:11864606]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:11843177]
EXISTENCE:mutant phenotype:GO:0000464 endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11843177]
EXISTENCE:mutant phenotype:GO:0000465 exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11843177]
Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
EXISTENCE:direct assay:GO:0031416 NatB complex [PMID:12783868]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:9105043]
EXISTENCE:genetic interaction:GO:0017196 N-terminal peptidyl-methionine acetylation [PMID:12783868]
EXISTENCE:genetic interaction:GO:0032956 regulation of actin cytoskeleton organization [PMID:12808144]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:9105043]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:12783868]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:9105043]
EXISTENCE:mutant phenotype:GO:0017196 N-terminal peptidyl-methionine acetylation [PMID:12783868]
EXISTENCE:mutant phenotype:GO:0032956 regulation of actin cytoskeleton organization [PMID:12808144]
Non-catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23658021]
EXISTENCE:direct assay:GO:0044695 Dsc E3 ubiquitin ligase complex [PMID:22681890]
Multi-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:12702719]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:12702719]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11139493]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12206772]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12411502]
EXISTENCE:direct assay:GO:0070390 transcription export complex 2 [PMID:15311284]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:11452010]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:18003937]
EXISTENCE:mutant phenotype:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17537816]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11139493]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:12411502]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:18614048]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:18003937]
Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
succinate dehydrogenase assembly factor SDH5
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:19628817]
EXISTENCE:direct assay:GO:0017013 protein flavinylation [PMID:19628817]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:12586695]
EXISTENCE:mutant phenotype:GO:0034553 mitochondrial respiratory chain complex II assembly [PMID:23043141]
EXISTENCE:physical interaction:GO:0006099 tricarboxylic acid cycle [PMID:19628817]
Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:25416945|PMID:30341817]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18086883|PMID:14562095]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:18086883|PMID:14562095]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:25416945]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:30341817]
EXISTENCE:direct assay:GO:0032177 cellular bud neck split septin rings [PMID:30341817]
EXISTENCE:genetic interaction:GO:2001107 negative regulation of Rho guanyl-nucleotide exchange factor activity [PMID:25416945]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:29074565]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:29074565]
EXISTENCE:mutant phenotype:GO:1901900 regulation of protein localization to cell division site [PMID:28148650]
Protein involved in axial bud site selection; prevents repolarization of cells at previous division sites; recruited by Aim44p and Nap1p to the cell cortex and to cytokinesis remnants (bud scars) where it prevents Rsr1p-mediated activation of Cdc24p, the Cdc42p GEF; localizes to the cytoplasm, and the cell division site prior to being stably recruited to cytokinesis remnants; establishes the transient localization of Rga1p, a Cdc42p GAP, at bud scars along with Nis1p; potential Cdc28p substrate
kinetochore-associated Ndc80 complex subunit NUF2
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11179222|PMID:11511347]
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:11266451]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:8188751]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11266451]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:17195848]
EXISTENCE:direct assay:GO:0031262 Ndc80 complex [PMID:11266451]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:11266451]
EXISTENCE:mutant phenotype:GO:0000776 kinetochore [PMID:11179222]
EXISTENCE:physical interaction:GO:0031262 Ndc80 complex [PMID:11179222]
Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
EXISTENCE:direct assay:GO:0017136 NAD-dependent histone deacetylase activity [PMID:11313477|PMID:11711434]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11313477|PMID:27185881]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11313477]
EXISTENCE:mutant phenotype:GO:0045950 negative regulation of mitotic recombination [PMID:16051752]
NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and meiotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:7493987|PMID:8422683]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10848632]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10848632]
EXISTENCE:direct assay:GO:0031930 mitochondria-nucleus signaling pathway [PMID:7493987]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7493987]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:8422683|PMID:7493987]
EXISTENCE:mutant phenotype:GO:0031930 mitochondria-nucleus signaling pathway [PMID:8422683]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12393187|PMID:8422683]
EXISTENCE:mutant phenotype:GO:0071400 cellular response to oleic acid [PMID:12393187]
Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization
bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:15466869]
EXISTENCE:direct assay:GO:0031119 tRNA pseudouridine synthesis [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:5366000|PMID:4555411]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0043723 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity [PMID:4555411]
Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:10660045]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11116155]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10660045]
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; role in secretion; localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
EXISTENCE:direct assay:GO:0008441 3'(2'),5'-bisphosphate nucleotidase activity [PMID:7809627]
EXISTENCE:direct assay:GO:0016078 tRNA catabolic process [PMID:31619505]
EXISTENCE:mutant phenotype:GO:0000103 sulfate assimilation [PMID:8910555]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:8393782]
EXISTENCE:mutant phenotype:GO:0042538 hyperosmotic salinity response [PMID:8393782]
Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway; human homolog BPNT1 complements yeast null mutant
EXISTENCE:genetic interaction:GO:0070651 nonfunctional rRNA decay [PMID:25534857]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:25534857]
Protein required for the degradation of mutant 25S rRNAs; functions in Mms1 pathway of nonfunctional 25S rRNA decay; expression induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat
EXISTENCE:curator inference:GO:0006886 intracellular protein transport [PMID:10564262]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:24460703]
EXISTENCE:physical interaction:GO:0030122 AP-2 adaptor complex [PMID:10564262]
Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals
ribose phosphate diphosphokinase subunit PRS5
EXISTENCE:direct assay:GO:0002189 ribose phosphate diphosphokinase complex [PMID:15280369]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:genetic interaction:GO:0004749 ribose phosphate diphosphokinase activity [PMID:15280369]
EXISTENCE:genetic interaction:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:15280369]
EXISTENCE:mutant phenotype:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:10212224]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:15470112]
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:10628851]
EXISTENCE:mutant phenotype:GO:0010961 cellular magnesium ion homeostasis [PMID:35087208]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:15498024]
Protein involved in magnesium homeostasis; required for Mg2+ sequestration in vacuole; required for normal mitochondrial morphology; has similarity to hemolysins
glycerol-3-phosphate dehydrogenase (NAD(+)) GPD2
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0006071 glycerol metabolic process [PMID:9171333]
EXISTENCE:mutant phenotype:GO:0006116 NADH oxidation [PMID:9171333]
EXISTENCE:mutant phenotype:GO:0047952 glycerol-3-phosphate dehydrogenase [NAD(P)+] activity [PMID:9171333]
NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004055 argininosuccinate synthase activity [PMID:2897249]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:205532]
EXISTENCE:mutant phenotype:GO:0006526 arginine biosynthetic process [PMID:2897249]
Argininosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0008239 dipeptidyl-peptidase activity [PMID:19825391]
Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
phosphoglycerate mutase family protein GPM3
Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16850348]
EXISTENCE:direct assay:GO:0008902 hydroxymethylpyrimidine kinase activity [PMID:15967475|PMID:15614489]
EXISTENCE:direct assay:GO:0008972 phosphomethylpyrimidine kinase activity [PMID:15614489]
EXISTENCE:direct assay:GO:0009230 thiamine catabolic process [PMID:15967475]
EXISTENCE:direct assay:GO:0050334 thiaminase activity [PMID:15967475]
EXISTENCE:genetic interaction:GO:0008902 hydroxymethylpyrimidine kinase activity [PMID:15614489]
EXISTENCE:genetic interaction:GO:0008972 phosphomethylpyrimidine kinase activity [PMID:10383756|PMID:15614489]
EXISTENCE:mutant phenotype:GO:0009228 thiamine biosynthetic process [PMID:10383756]
Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:21070970]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:21070970|PMID:21070971]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:21070970|PMID:21070971]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:21070970|PMID:21070971]
E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p; ubiquitination of Cse4p may be antagonized by Scm3p; interacts with the FACT complex preventing mislocalization of Cse4p to euchromatin independent of Slx5p; formyl-methionine/N-recognin component of N-end rule pathway mediating destruction of formylated cytosolic proteins elevated in response to starvation or stationary phase entry
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
hypothetical protein; null mutant displays elevated frequency of mitochondrial genome loss; localizes to mitochondria, cytosol and to the peroxisome in a Pex5p-dependent manner in glucose
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:expression pattern:GO:0006974 cellular response to DNA damage stimulus [PMID:9473471]
EXISTENCE:expression pattern:GO:0009267 cellular response to starvation [PMID:9473471]
EXISTENCE:expression pattern:GO:0034605 cellular response to heat [PMID:9473471]
EXISTENCE:expression pattern:GO:0070301 cellular response to hydrogen peroxide [PMID:9473471]
Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004014 adenosylmethionine decarboxylase activity [PMID:2266128]
EXISTENCE:mutant phenotype:GO:0006597 spermine biosynthetic process [PMID:7929015]
EXISTENCE:mutant phenotype:GO:0008295 spermidine biosynthetic process [PMID:7929015]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:curator inference:GO:0031429 box H/ACA snoRNP complex [PMID:15306656]
EXISTENCE:direct assay:GO:0030565 snRNA pseudouridylation guide activity [PMID:15962000]
EXISTENCE:direct assay:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1052, constitutive pseudouridylation of U2 snRNA (LSR1) at position 42, and stress-induced U2 pseudouridylation at position 93
EXISTENCE:direct assay:GO:0001095 TFIIE-class transcription factor complex binding [PMID:8790357]
EXISTENCE:direct assay:GO:0001097 TFIIH-class transcription factor complex binding [PMID:10973956]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:8378310]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8187178]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12482986|PMID:8378310]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:20308326]
EXISTENCE:genetic interaction:GO:0070202 regulation of establishment of protein localization to chromosome [PMID:23447536]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11470794]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15254252]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0070202 regulation of establishment of protein localization to chromosome [PMID:23447536]
EXISTENCE:mutant phenotype:GO:2000219 positive regulation of invasive growth in response to glucose limitation [PMID:18202364]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
EXISTENCE:direct assay:GO:0004363 glutathione synthase activity [PMID:9512666]
EXISTENCE:mutant phenotype:GO:0006750 glutathione biosynthetic process [PMID:9512666]
Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:23966878]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:23966878]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:23966878]
Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005633 ascus lipid droplet [PMID:23966878]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24390141]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds1p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:12372297]
EXISTENCE:genetic interaction:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:12372297]
EXISTENCE:genetic interaction:GO:0060917 regulation of (1->6)-beta-D-glucan biosynthetic process [PMID:17531808]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:12372297]
EXISTENCE:mutant phenotype:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:12372297]
EXISTENCE:mutant phenotype:GO:2000766 negative regulation of cytoplasmic translation [PMID:26310304]
PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:9405362]
EXISTENCE:genetic interaction:GO:0043495 protein-membrane adaptor activity [PMID:12808025]
EXISTENCE:mutant phenotype:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:12808025]
EXISTENCE:physical interaction:GO:0043495 protein-membrane adaptor activity [PMID:12808025|PMID:16007078|PMID:16911527]
Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly
bifunctional N-glycosylase/AP lyase NTG2
EXISTENCE:direct assay:GO:0000703 oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity [PMID:9020769]
EXISTENCE:direct assay:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:14500818]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10471279|PMID:19029246]
EXISTENCE:direct assay:GO:0006284 base-excision repair [PMID:14500818]
EXISTENCE:direct assay:GO:0006285 base-excision repair, AP site formation [PMID:9826748]
DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11452019]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0030684 preribosome [PMID:17922018]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21811236|PMID:11452019]
Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:15167894|PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:1601875]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:9559554]
Ribosomal protein P2 alpha; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
hypothetical protein; identified by SAGE analysis
proteasome core particle subunit alpha 4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18504300]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16806052]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019773 proteasome core complex, alpha-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:9799224]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
hypothetical protein; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position G867
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:16793545]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:21293191]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11927594]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:11927594]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:21293191]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391]
EXISTENCE:direct assay:GO:0030915 Smc5-Smc6 complex [PMID:15738391]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391]
EXISTENCE:mutant phenotype:GO:0051304 chromosome separation [PMID:15793567]
EXISTENCE:mutant phenotype:GO:0071139 resolution of recombination intermediates [PMID:20159973]
EXISTENCE:physical interaction:GO:0000725 recombinational repair [PMID:19995966]
Subunit of the SMC5-SMC6 complex; the SMC5-SMC6 complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; supports nucleolar function; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
EXISTENCE:curator inference:GO:0070149 mitochondrial glutamyl-tRNA aminoacylation [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7607232]
Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated
EXISTENCE:mutant phenotype:GO:0006020 inositol metabolic process [PMID:16582425]
Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:10958688]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:10958688]
Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein
EXISTENCE:curator inference:GO:0006074 (1->3)-beta-D-glucan metabolic process [PMID:10769178|PMID:17397106]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953|PMID:15781460]
1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall
hypothetical protein; separated from neighboring gene, YAP7, by frame shift and corresponds to C-terminal part of YAP7 orthologs in other species; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:20802496]
Transcriptional repressor of nitric oxide oxidase; basic leucine zipper (bZIP) transcription factor; neighboring gene, YOL029C, created by frame-shift at position 708, corresponds to C-terminal part of YAP7 orthologs in other species; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15138253|PMID:16476776]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:20427570]
EXISTENCE:genetic interaction:GO:0006813 potassium ion transport [PMID:20197279]
EXISTENCE:genetic interaction:GO:0070131 positive regulation of mitochondrial translation [PMID:20427570]
EXISTENCE:genetic interaction:GO:1902600 proton transmembrane transport [PMID:20197279]
EXISTENCE:mutant phenotype:GO:0006813 potassium ion transport [PMID:15904662]
EXISTENCE:mutant phenotype:GO:0030007 cellular potassium ion homeostasis [PMID:15138253]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:16476776]
Membrane-associated mitochondrial ribosome receptor; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; ribosome-associated protein involved in the insertion of newly synthesized proteins into the mitochondrial inner membrane; role in protein export and K+/H+ exchange; localizes to the inner mitochondrial membrane; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:15608614|PMID:15326197]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:21825073|PMID:21825074]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:15608614|PMID:17974559]
Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; forms a complex with Mim2p in the outer membrane that functions as cation-selective channel for translocation of positively charged precursor segments; also has a role in assembly of Tom20p into the TOM complex
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:genetic interaction:GO:0051444 negative regulation of ubiquitin-protein transferase activity [PMID:19942853]
EXISTENCE:mutant phenotype:GO:0051444 negative regulation of ubiquitin-protein transferase activity [PMID:19763088]
Protein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1
hypothetical protein; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication
ARS1511; Autonomously Replicating Sequence; replication capacity dependent upon Sum1p
EXISTENCE:curator inference:GO:0070124 mitochondrial translational initiation [PMID:15935987]
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:12729623]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:15935987]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:12729623]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:11914276|PMID:14576278|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:1935960]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:1935960]
Mitochondrial translation initiation factor 2
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:31182640]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:31062022|PMID:31182640]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:31062022]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:19806183]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:31062022|PMID:31182640]
EXISTENCE:physical interaction:GO:0051082 unfolded protein binding [PMID:31182640]
Cytoplasmic assembly chaperone for ribosomal protein Rps2p; co-translationally associates with the N-terminal extension of Rps2p to facilitate expression, solubility and ribosome assembly; released prior to the nuclear import of Rps2p; required for correct processing of 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in the S288C background but not in CEN.PK2; homologous to human PDCD2L and PDCD2
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:17643380]
EXISTENCE:direct assay:GO:0000175 3'-5'-exoribonuclease activity [PMID:17173052|PMID:17643380]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:19060886|PMID:19060898|PMID:19129231]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9562621|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000049 tRNA binding [PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000175 3'-5'-exoribonuclease activity [PMID:17173052|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10508172|PMID:9390555]
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:19060886|PMID:19129231]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0016075 rRNA catabolic process [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0043628 regulatory ncRNA 3'-end processing [PMID:11352936]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19481524]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:11030620]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:16431988|PMID:18940861]
EXISTENCE:mutant phenotype:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:17643380]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:15935759]
Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress
aromatic amino acid transmembrane transporter TAT2
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12810702|PMID:30660361]
EXISTENCE:direct assay:GO:0015173 aromatic amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0015801 aromatic amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:35031272]
EXISTENCE:mutant phenotype:GO:0005300 high-affinity tryptophan transmembrane transporter activity [PMID:7523855|PMID:35031272]
EXISTENCE:mutant phenotype:GO:0015827 tryptophan transport [PMID:7523855|PMID:35031272]
High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:33002606]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:33002606|PMID:15282802]
Protein involved in secretion and cell wall organization; localizes to plasma membrane microdomains called MCCs (membrane compartment occupied by Can1); GFP-fusion protein localizes to the cell periphery and vacuole; Sur7/PalI family transmembrane domain (TMD) containing protein; TOS7 has a paralog, DCV1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11739407]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:9427746|PMID:9565594]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11739407]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:9565594]
EXISTENCE:genetic interaction:GO:0006673 inositol phosphoceramide metabolic process [PMID:18753406]
EXISTENCE:genetic interaction:GO:0006675 mannosyl-inositol phosphorylceramide metabolic process [PMID:18753406]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:18753406]
EXISTENCE:genetic interaction:GO:0032527 protein exit from endoplasmic reticulum [PMID:18753406]
EXISTENCE:mutant phenotype:GO:0006673 inositol phosphoceramide metabolic process [PMID:18753406]
EXISTENCE:mutant phenotype:GO:0006675 mannosyl-inositol phosphorylceramide metabolic process [PMID:18753406]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:9427746]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:22753847]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10545112]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:10397773]
Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; required along with VPS45 for an early step of the constitutive CVT pathway; interactions with Vps45 prevents Tlg2p degradation, and facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:27185881]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:12399377]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:12399377]
Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
calmodulin-dependent protein kinase CMK2
EXISTENCE:direct assay:GO:0004683 calmodulin-dependent protein kinase activity [PMID:2061341]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:2061341|PMID:16319894]
Calmodulin-dependent protein kinase; negative feedback controller of calcium/calcineurin signalling pathway, also has additional Crz1-independent role in promoting calcium tolerance; amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070056 prospore membrane leading edge [PMID:24390141]
hypothetical protein; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; null mutant displays increased levels of spontaneous Rad52p foci
hypothetical protein; mCherry fusion protein localizes to the cytosol and nucleus
hypothetical protein; identified by SAGE
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0000838 Hrd1p ubiquitin ligase ERAD-M complex [PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:11139575|PMID:11146622]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:27831485]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11139575|PMID:9437001]
EXISTENCE:direct assay:GO:0030970 retrograde protein transport, ER to cytosol [PMID:27321670]
EXISTENCE:direct assay:GO:0051865 protein autoubiquitination [PMID:27321670|PMID:31713515]
EXISTENCE:genetic interaction:GO:0004842 ubiquitin-protein transferase activity [PMID:11146622]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:18971375]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:11146622]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:17035235|PMID:22298424|PMID:11146622]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:22298424]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:24292014]
Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) of misfolded proteins; upon autoubiquitination triggers retrotranslocation of misfolded proteins to cytosol for degradation; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11081628|PMID:11090616]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11081628]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11081628]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11090616]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:21357739]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11081628]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:17660569]
Histone variant H2AZ; exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin; Htz1p-containing nucleosomes facilitate RNA Pol II passage by affecting correct assembly and modification status of RNA Pol II elongation complexes and by favoring efficient nucleosome remodeling
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:10497163]
EXISTENCE:direct assay:GO:0006660 phosphatidylserine catabolic process [PMID:10497163]
EXISTENCE:direct assay:GO:0046488 phosphatidylinositol metabolic process [PMID:10497163]
EXISTENCE:mutant phenotype:GO:0004622 lysophospholipase activity [PMID:10497163]
EXISTENCE:mutant phenotype:GO:0006660 phosphatidylserine catabolic process [PMID:10497163]
EXISTENCE:mutant phenotype:GO:0046488 phosphatidylinositol metabolic process [PMID:10497163]
Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21849504]
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:21849504]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10790377]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:16307926]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:16307926|PMID:12150911]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21849504|PMID:10790377]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10790377]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10790377]
Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:9024686]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0008429 phosphatidylethanolamine binding [PMID:27821511]
EXISTENCE:direct assay:GO:0031210 phosphatidylcholine binding [PMID:27821511]
EXISTENCE:direct assay:GO:0120013 lipid transfer activity [PMID:29279306]
EXISTENCE:direct assay:GO:1901611 phosphatidylglycerol binding [PMID:27821511]
EXISTENCE:genetic interaction:GO:0015914 phospholipid transport [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:9024686]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:30908556]
EXISTENCE:mutant phenotype:GO:0015917 aminophospholipid transport [PMID:27469264]
EXISTENCE:mutant phenotype:GO:0031210 phosphatidylcholine binding [PMID:27821511]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:17410204]
EXISTENCE:mutant phenotype:GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering [PMID:19556461]
EXISTENCE:physical interaction:GO:0032865 ERMES complex [PMID:13679517|PMID:17410204]
Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
ARS1512; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:16230336]
EXISTENCE:direct assay:GO:0048039 ubiquinone binding [PMID:16230336]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:23270816]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:23270816|PMID:50596]
Coenzyme Q (ubiquinone) binding protein; functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes; respiratory growth defect of the null mutant is functionally complemented by human COQ10A
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:15282802|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15282802]
hypothetical protein; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
EXISTENCE:direct assay:GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity [PMID:6088500|PMID:6319395]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:6088500]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:6088500]
EXISTENCE:genetic interaction:GO:0009303 rRNA transcription [PMID:2436053]
EXISTENCE:mutant phenotype:GO:0000019 regulation of mitotic recombination [PMID:2902925]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:10082585]
EXISTENCE:mutant phenotype:GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity [PMID:2989818|PMID:6319395]
EXISTENCE:mutant phenotype:GO:0006265 DNA topological change [PMID:2989818]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:2549254]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:9199287]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:1660829]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:2840207]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:8895658]
EXISTENCE:mutant phenotype:GO:0007097 nuclear migration [PMID:8895658]
EXISTENCE:physical interaction:GO:0005730 nucleolus [PMID:10967121]
Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
DNA-directed RNA polymerase II core subunit RPB11
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:17179178]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:16537912]
RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
EXISTENCE:direct assay:GO:0032221 Rpd3S/Clr6-CII complex [PMID:16286007|PMID:16286008]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:direct assay:GO:0070822 Sin3-type complex [PMID:9234741]
EXISTENCE:genetic interaction:GO:0000117 regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:17908798]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11095743|PMID:2690066]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:14737171]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:9150136]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:14711989]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19270272]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:15143171]
EXISTENCE:mutant phenotype:GO:0044804 autophagy of nucleus [PMID:31553911]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11095743|PMID:14737171|PMID:20398213]
EXISTENCE:mutant phenotype:GO:0051038 negative regulation of transcription involved in meiotic cell cycle [PMID:17158929]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:10388812]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:10082585|PMID:10388812]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:20398213]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:31553911]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:9150136]
Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16647879|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0016409 palmitoyltransferase activity [PMID:16818716|PMID:26224664]
EXISTENCE:mutant phenotype:GO:0018345 protein palmitoylation [PMID:16751107|PMID:16818716]
Palmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18625204]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15664187]
EXISTENCE:mutant phenotype:GO:0009636 response to toxic substance [PMID:15664187]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15664187]
Plasma membrane receptor for plant antifungal osmotin; involved in zinc ion homeostasis, apoptosis; negatively regulates ZRT1 and other functionally divergent genes through CCCTC promoter motif (IzRE); modulates FET3 activity in iron-independent manner; affects gene expression by influencing balance of competition between Msn2p/Msn4p and Nrg1p/Nrg2p for binding to IzRE; transcription regulated by Zap1p, zinc, fatty acid levels; homolog of mammalian adiponectin receptor
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:8108735]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9853758]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:8108735]
EXISTENCE:direct assay:GO:1990860 Pho85-Pho80 CDK-cyclin complex [PMID:8108735]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:3915785]
EXISTENCE:genetic interaction:GO:0045936 negative regulation of phosphate metabolic process [PMID:3915785]
EXISTENCE:genetic interaction:GO:0050849 negative regulation of calcium-mediated signaling [PMID:16455487]
EXISTENCE:mutant phenotype:GO:0006875 cellular metal ion homeostasis [PMID:20429018]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:20417603]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:16455487]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:27252384]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:8108735]
Cyclin; interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
EXISTENCE:direct assay:GO:0000175 3'-5'-exoribonuclease activity [PMID:10611239]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10611239|PMID:23580640]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16541108|PMID:31662437]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:31662437]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860]
EXISTENCE:genetic interaction:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172|PMID:10716935]
EXISTENCE:genetic interaction:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:12417728|PMID:17410208|PMID:11586364]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:16541108]
EXISTENCE:genetic interaction:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:18456844]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172|PMID:18940861|PMID:9582370]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:18614049]
EXISTENCE:mutant phenotype:GO:0032204 regulation of telomere maintenance [PMID:28160602]
EXISTENCE:mutant phenotype:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172|PMID:10611222|PMID:16373491]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:27124216]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791|PMID:15572680|PMID:16431988|PMID:18007593|P MID:18940861|PMID:15173578]
EXISTENCE:mutant phenotype:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:16373491]
EXISTENCE:mutant phenotype:GO:0071037 nuclear polyadenylation-dependent snRNA catabolic process [PMID:16431988]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:15935759|PMID:16973436|PMID:18007593]
EXISTENCE:mutant phenotype:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:16373491|PMID:19369424]
EXISTENCE:mutant phenotype:GO:0071044 histone mRNA catabolic process [PMID:17179095]
EXISTENCE:mutant phenotype:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10611222|PMID:16373491|PMID:18951092]
Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based
dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:8877369]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:8877369]
EXISTENCE:mutant phenotype:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:10336995]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:15794922]
EXISTENCE:mutant phenotype:GO:0016758 hexosyltransferase activity [PMID:8877369]
EXISTENCE:mutant phenotype:GO:0042281 dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity [PMID:10336995]
ER-luminal alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; C998T transition in human ortholog ALG6 causes carbohydrate-deficient glycoprotein syndrome type-Ic; wild-type human ortholog ALG6 can partially complement yeast alg6 mutant
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:17651441]
Putative precursor of the subtilisin-like protease III; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YSP3 has a paralog, PRB1, that arose from the whole genome duplication
rRNA-binding ribosome biosynthesis protein UTP23
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16769905|PMID:14690591]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:16769905]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:24152547]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16769905]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16769905]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16769905]
EXISTENCE:mutant phenotype:GO:0070181 small ribosomal subunit rRNA binding [PMID:24152547]
EXISTENCE:physical interaction:GO:0032040 small-subunit processome [PMID:16769905]
Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
EXISTENCE:direct assay:GO:0003910 DNA ligase (ATP) activity [PMID:9242411|PMID:9271115]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15342630]
EXISTENCE:mutant phenotype:GO:0003910 DNA ligase (ATP) activity [PMID:9271115]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:9242411]
EXISTENCE:mutant phenotype:GO:0043007 maintenance of rDNA [PMID:20541983]
EXISTENCE:physical interaction:GO:0032807 DNA ligase IV complex [PMID:17567543]
DNA ligase required for nonhomologous end-joining (NHEJ); forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth; mutations in human ortholog lead to ligase IV syndrome and Dubowitz syndrome
ARS1513; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:mutant phenotype:GO:0106388 18S rRNA aminocarboxypropyltransferase activity [PMID:27084949]
Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress; also detected in peroxisomes
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:12482202]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:20850366]
Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9391108|PMID:20652590]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17761172]
EXISTENCE:genetic interaction:GO:0004888 transmembrane signaling receptor activity [PMID:10430578]
EXISTENCE:genetic interaction:GO:0006970 response to osmotic stress [PMID:14871941]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:30361468]
EXISTENCE:mutant phenotype:GO:0007266 Rho protein signal transduction [PMID:10348843|PMID:10508863]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10348843|PMID:10508863]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:10508863|PMID:24326069]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:10508863]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:14593073|PMID:24326069]
Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
hypothetical protein; includes a potential transmembrane domain; deletion results in slightly lengthened telomeres; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
Cell wall mannoprotein; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
EXISTENCE:direct assay:GO:0071497 cellular response to freezing [PMID:7746155]
Putative cell wall mannoprotein; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis; TIR2 has a paralog, TIR3, that arose from the whole genome duplication
ATP-binding cassette sterol transporter AUS1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21521689]
EXISTENCE:genetic interaction:GO:0035376 sterol import [PMID:21110944|PMID:12077145]
EXISTENCE:mutant phenotype:GO:0034041 ABC-type sterol transporter activity [PMID:21521689]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:21521689|PMID:12077145]
Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication
protein phosphatase 2A regulatory subunit RTS1
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:25236599]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:12636916|PMID:23785440]
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:16541024]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:31781172]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12388751|PMID:12636916|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:12388751]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12388751|PMID:12636916]
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:9079645]
EXISTENCE:genetic interaction:GO:0031134 sister chromatid biorientation [PMID:25236599]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:19605686]
EXISTENCE:genetic interaction:GO:0032186 cellular bud neck septin ring organization [PMID:12636916]
EXISTENCE:genetic interaction:GO:2000786 positive regulation of autophagosome assembly [PMID:27973551]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:31781172]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:19605686]
EXISTENCE:mutant phenotype:GO:0031107 septin ring disassembly [PMID:12636916]
EXISTENCE:mutant phenotype:GO:0032186 cellular bud neck septin ring organization [PMID:12636916]
EXISTENCE:mutant phenotype:GO:0051754 meiotic sister chromatid cohesion, centromeric [PMID:16541024]
EXISTENCE:mutant phenotype:GO:0070199 establishment of protein localization to chromosome [PMID:24945276]
B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
hypothetical protein; conserved among S. cerevisiae strains; YOR015W is not an essential gene
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10359606]
Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:9111353]
EXISTENCE:mutant phenotype:GO:0000964 mitochondrial RNA 5'-end processing [PMID:18086665|PMID:9111353]
Protein with a role in 5'-end processing of mitochondrial RNAs; located in the mitochondrial membrane
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8621680]
EXISTENCE:direct assay:GO:0031625 ubiquitin protein ligase binding [PMID:24820415]
EXISTENCE:direct assay:GO:0071333 cellular response to glucose stimulus [PMID:22249293]
EXISTENCE:genetic interaction:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:24820415]
EXISTENCE:genetic interaction:GO:0002092 positive regulation of receptor internalization [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:12163175]
EXISTENCE:mutant phenotype:GO:0031625 ubiquitin protein ligase binding [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:22249293]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Alpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
hypothetical protein; may interact with ribosomes, based on co-purification experiments; YOR019W has a paralog, JIP4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:7903252]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:7902576]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9256426]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:7902576]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:9256426]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:7913473]
EXISTENCE:mutant phenotype:GO:0051131 chaperone-mediated protein complex assembly [PMID:7913473]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:9256426]
Mitochondrial matrix co-chaperonin; inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
Mitochondrial hypothetical protein; conserved in A. gossypii; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
protein-arginine N-methyltransferase SFM1
EXISTENCE:direct assay:GO:0035241 protein-arginine omega-N monomethyltransferase activity [PMID:22650761]
EXISTENCE:mutant phenotype:GO:0018216 peptidyl-arginine methylation [PMID:22650761]
SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
EXISTENCE:direct assay:GO:0004620 phospholipase activity [PMID:27402848|PMID:27746179]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:27746179]
EXISTENCE:mutant phenotype:GO:0004620 phospholipase activity [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0032048 cardiolipin metabolic process [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0046337 phosphatidylethanolamine metabolic process [PMID:27402848]
DDHD domain-containing phospholipase A1; mitochondrial matrix enzyme with sn-1-specific activity, hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in remodeling of mitochondrial phospholipids; antagonistically regulated by Aft1p and Aft2p; in humans, mutations in DDHD1 and DDHD2 genes cause specific types of hereditary spastic paraplegia, while DDL1-defective yeast share similar phenotypes such as mitochondrial dysfunction and defects in lipid metabolism
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:10490601]
EXISTENCE:direct assay:GO:0140671 ADA complex [PMID:10490601]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:10490601]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:23357952]
Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; Ahc2p may tether Ahc1p to the complex
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:12242223]
EXISTENCE:direct assay:GO:0009299 mRNA transcription [PMID:31167142]
EXISTENCE:direct assay:GO:0017136 NAD-dependent histone deacetylase activity [PMID:17977840]
EXISTENCE:direct assay:GO:0098781 ncRNA transcription [PMID:31167142]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:7498786]
EXISTENCE:mutant phenotype:GO:0017136 NAD-dependent histone deacetylase activity [PMID:17977840]
EXISTENCE:mutant phenotype:GO:0046459 short-chain fatty acid metabolic process [PMID:12618394]
Sirtuin, histone H3 deacetylase; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; member of the Sir2 family of NAD(+)-dependent protein deacetylases
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:27170178]
EXISTENCE:direct assay:GO:0033597 mitotic checkpoint complex [PMID:11726501]
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:22940250]
EXISTENCE:genetic interaction:GO:0044774 mitotic DNA integrity checkpoint signaling [PMID:16651657]
EXISTENCE:physical interaction:GO:0033597 mitotic checkpoint complex [PMID:10704439|PMID:11726501]
Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0030544 Hsp70 protein binding [PMID:12716905]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:21170051]
EXISTENCE:direct assay:GO:0051879 Hsp90 protein binding [PMID:9927435|PMID:12525481]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:8972212]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:27412066]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:24109600]
EXISTENCE:physical interaction:GO:0005737 cytoplasm [PMID:12525481]
Evolutionarily-conserved Hsp90 cochaperone; regulates spatial organization of amyloid-like proteins in the cytosol, thereby buffering the proteotoxicity caused by amyloid-like proteins; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11179441|PMID:19029946]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19029946]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:20802496|PMID:9372930|PMID:19111667]
EXISTENCE:direct assay:GO:0140297 DNA-binding transcription factor binding [PMID:21552514]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:19774548]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:11179441|PMID:15821130|PMID:9593122]
EXISTENCE:mutant phenotype:GO:0042538 hyperosmotic salinity response [PMID:9559673]
Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YOR029W is not an essential gene
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23019326]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:15879523]
Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:17163970]
EXISTENCE:mutant phenotype:GO:0005507 copper ion binding [PMID:7929222]
EXISTENCE:mutant phenotype:GO:0010038 response to metal ion [PMID:17163970|PMID:7929222]
Copper-binding metallothionein; required for wild-type copper resistance
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:9832522]
bHLH protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
hypothetical protein; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0008409 5'-3' exonuclease activity [PMID:12531018]
EXISTENCE:direct assay:GO:0017108 5'-flap endonuclease activity [PMID:12531018]
EXISTENCE:genetic interaction:GO:0000706 meiotic DNA double-strand break processing [PMID:20150422]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:15126387|PMID:19595717]
EXISTENCE:genetic interaction:GO:0000729 DNA double-strand break processing [PMID:17347674|PMID:21841787|PMID:18805091]
EXISTENCE:genetic interaction:GO:0031860 telomeric 3' overhang formation [PMID:20808892]
EXISTENCE:genetic interaction:GO:0044818 mitotic G2/M transition checkpoint [PMID:18805091]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:17347674]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:11438669]
EXISTENCE:mutant phenotype:GO:0007534 gene conversion at mating-type locus [PMID:18806779]
5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:16647879]
Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8625407]
EXISTENCE:direct assay:GO:0017022 myosin binding [PMID:12725728]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:8625407]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:11553699]
Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:14981247]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:1493335]
EXISTENCE:mutant phenotype:GO:0005484 SNAP receptor activity [PMID:8730101]
EXISTENCE:mutant phenotype:GO:0005768 endosome [PMID:8730101]
EXISTENCE:mutant phenotype:GO:0005794 Golgi apparatus [PMID:8730101]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:8730101]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
Target membrane receptor (t-SNARE); for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14562095|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:16207709]
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:16207709]
EXISTENCE:genetic interaction:GO:0018063 cytochrome c-heme linkage [PMID:14514677|PMID:16207709]
EXISTENCE:mutant phenotype:GO:0007006 mitochondrial membrane organization [PMID:11488609]
Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9001207]
EXISTENCE:direct assay:GO:0000417 HIR complex [PMID:16264190|PMID:16303565]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16264190]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9001207]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8417331|PMID:22932476]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:16264190|PMID:16303565]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16449659]
EXISTENCE:genetic interaction:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:23962978]
EXISTENCE:mutant phenotype:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:23962978]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:16264190]
EXISTENCE:physical interaction:GO:0000417 HIR complex [PMID:16264190]
Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005956 protein kinase CK2 complex [PMID:8135547]
EXISTENCE:direct assay:GO:0006356 regulation of transcription by RNA polymerase I [PMID:11551505]
EXISTENCE:direct assay:GO:0006359 regulation of transcription by RNA polymerase III [PMID:11551505]
EXISTENCE:direct assay:GO:0006974 cellular response to DNA damage stimulus [PMID:11551505]
EXISTENCE:direct assay:GO:0030291 protein serine/threonine kinase inhibitor activity [PMID:18265947]
EXISTENCE:direct assay:GO:0032545 CURI complex [PMID:17452446]
EXISTENCE:direct assay:GO:0034456 UTP-C complex [PMID:17515605]
EXISTENCE:mutant phenotype:GO:0019887 protein kinase regulator activity [PMID:11827175]
Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
EXISTENCE:direct assay:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:9261170]
EXISTENCE:direct assay:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
EXISTENCE:genetic interaction:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:genetic interaction:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
EXISTENCE:mutant phenotype:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:mutant phenotype:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2340 and U2345
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12628920]
EXISTENCE:direct assay:GO:0070530 K63-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25042851]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:25042851]
Ubiquitin-binding protein; functions as a Ub-Atg8p adaptor in ubiquitin-dependent autophagy; serves as a proteaphagy receptor for inactivated 26S proteasomes; contains a CUE domain that binds K48-and K63-linked Ub chains and also binds monoubiquitin, facilitating intramolecular monoubiquitination; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U1888
EXISTENCE:direct assay:GO:0019903 protein phosphatase binding [PMID:12090248]
EXISTENCE:genetic interaction:GO:1904262 negative regulation of TORC1 signaling [PMID:30142151]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:21429936]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:15020461]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:15020461]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:12090248]
EXISTENCE:mutant phenotype:GO:1903452 positive regulation of G1 to G0 transition [PMID:7001255]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:30142151]
Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
hypothetical protein involved in multidrug resistance; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:7565772]
EXISTENCE:direct assay:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:20668160]
EXISTENCE:mutant phenotype:GO:0005742 mitochondrial outer membrane translocase complex [PMID:9774667|PMID:7565772]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:9774667]
Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
EXISTENCE:direct assay:GO:0000822 inositol hexakisphosphate binding [PMID:16783363]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:9564047]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15280434]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10610322|PMID:15280434|PMID:9564048]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:17272721]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:19198597]
EXISTENCE:direct assay:GO:0006409 tRNA export from nucleus [PMID:31453808]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:19805289]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:27251550]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:10610322]
EXISTENCE:genetic interaction:GO:0006415 translational termination [PMID:17272721]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:9564048]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342]
EXISTENCE:physical interaction:GO:0006415 translational termination [PMID:17272721]
Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10373505]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10373505]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:12618390]
EXISTENCE:genetic interaction:GO:0071590 nicotinamide riboside biosynthetic process [PMID:19846558]
EXISTENCE:genetic interaction:GO:0071592 nicotinic acid riboside biosynthetic process [PMID:19846558]
EXISTENCE:mutant phenotype:GO:0006006 glucose metabolic process [PMID:10373505]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:7667094]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:10373505]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:9725828]
EXISTENCE:mutant phenotype:GO:0071590 nicotinamide riboside biosynthetic process [PMID:19846558]
EXISTENCE:mutant phenotype:GO:0071592 nicotinic acid riboside biosynthetic process [PMID:19846558]
Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]; STD1 has a paralog, MTH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:26638174]
EXISTENCE:direct assay:GO:0004534 5'-3' exoribonuclease activity [PMID:8417335]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9315672|PMID:22842922]
EXISTENCE:direct assay:GO:0110155 NAD-cap decapping [PMID:35173156]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:9488433]
EXISTENCE:genetic interaction:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:19818713]
EXISTENCE:genetic interaction:GO:0034244 negative regulation of transcription elongation by RNA polymerase II [PMID:24501251]
EXISTENCE:genetic interaction:GO:0043144 sno(s)RNA processing [PMID:9488433]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:11030620]
EXISTENCE:genetic interaction:GO:1901408 negative regulation of phosphorylation of RNA polymerase II C-terminal domain [PMID:24501251]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:33978753]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11142370|PMID:9488433]
EXISTENCE:mutant phenotype:GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled [PMID:15565157]
EXISTENCE:mutant phenotype:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:19818713]
EXISTENCE:mutant phenotype:GO:0034244 negative regulation of transcription elongation by RNA polymerase II [PMID:24501251]
EXISTENCE:mutant phenotype:GO:0043144 sno(s)RNA processing [PMID:9488433]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:11030620|PMID:27124216]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:16131592]
EXISTENCE:mutant phenotype:GO:1901408 negative regulation of phosphorylation of RNA polymerase II C-terminal domain [PMID:24501251]
Nuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snoRNA processing, as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage; displaces Cdk1p from elongating transcripts, especially as RNAPII reaches the poly(A) site, negatively regulates phosphorylation of the CTD of RNAPII, and inhibits RNAPII transcriptional elongation
EXISTENCE:direct assay:GO:0005324 long-chain fatty acid transporter activity [PMID:28175317]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12034738]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12034738|PMID:16407254]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12034738]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:24146988]
EXISTENCE:direct assay:GO:1905329 sphingoid long-chain base transport [PMID:12034738]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:15342785]
EXISTENCE:mutant phenotype:GO:1905329 sphingoid long-chain base transport [PMID:12034738]
Sphingoid long-chain base (LCB) efflux transporter; integral membrane transporter that localizes to the plasma membrane and may transport long chain bases (LCBs) from the cytoplasmic side toward the extracytoplasmic side of the membrane; role in glycerophospholipid translocation; suppressor of the sphingoid LCB sensitivity of an LCB-lyase mutation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:14562095|PMID:26359986]
EXISTENCE:genetic interaction:GO:0006415 translational termination [PMID:20630870]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:20630870]
Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
EXISTENCE:direct assay:GO:0071218 cellular response to misfolded protein [PMID:27226598]
EXISTENCE:direct assay:GO:0071243 cellular response to arsenic-containing substance [PMID:27226598]
EXISTENCE:mutant phenotype:GO:0071243 cellular response to arsenic-containing substance [PMID:24297164]
AN1-type zinc finger protein, effector of proteotoxic stress response; stress-inducible transcriptional target of Rpn4p; induced by nitrogen limitation, weak acid, misfolded proteins; short-lived protein, degraded by proteasome; may protect cells from trivalent metalloid induced proteotoxicity; contains PACE promoter element; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases under DNA replication stress
phosphopantothenoylcysteine decarboxylase complex subunit VHS3
EXISTENCE:direct assay:GO:0004633 phosphopantothenoylcysteine decarboxylase activity [PMID:19915539]
EXISTENCE:direct assay:GO:0004864 protein phosphatase inhibitor activity [PMID:15192104]
EXISTENCE:direct assay:GO:0071513 phosphopantothenoylcysteine decarboxylase complex [PMID:19915539]
EXISTENCE:direct assay:GO:1990143 CoA-synthesizing protein complex [PMID:23789928]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19915539]
EXISTENCE:genetic interaction:GO:0030004 cellular monovalent inorganic cation homeostasis [PMID:15192104]
EXISTENCE:mutant phenotype:GO:0004633 phosphopantothenoylcysteine decarboxylase activity [PMID:19915539]
EXISTENCE:physical interaction:GO:0030004 cellular monovalent inorganic cation homeostasis [PMID:15192104]
Negative regulatory subunit of protein phosphatase 1 Ppz1p; involved in coenzyme A biosynthesis; subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p)
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:19801658]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12502737|PMID:22842922]
EXISTENCE:direct assay:GO:0030490 maturation of SSU-rRNA [PMID:19801658]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:12588997]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:19706509]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12588997|PMID:15388878]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:12502737]
EXISTENCE:physical interaction:GO:0043248 proteasome assembly [PMID:12502737]
Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
EXISTENCE:direct assay:GO:0000151 ubiquitin ligase complex [PMID:10445024]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:34192536]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:34192536]
EXISTENCE:direct assay:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:34192536]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:16945921]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:16945921|PMID:34192536]
EXISTENCE:direct assay:GO:0051382 kinetochore assembly [PMID:10445024]
EXISTENCE:direct assay:GO:0065003 protein-containing complex assembly [PMID:15090617]
Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0001578 microtubule bundle formation [PMID:12591913]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:7559759]
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:22842922]
EXISTENCE:direct assay:GO:0005880 nuclear microtubule [PMID:7559759]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:12591913]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:7559759]
EXISTENCE:genetic interaction:GO:0000022 mitotic spindle elongation [PMID:7559759]
EXISTENCE:genetic interaction:GO:0000073 initial mitotic spindle pole body separation [PMID:16688214]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:12591913|PMID:7559759]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:16615892]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:9036857]
EXISTENCE:mutant phenotype:GO:0051255 spindle midzone assembly [PMID:17562791]
Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0004622 lysophospholipase activity [PMID:25014274]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093|PMID:25014274]
EXISTENCE:mutant phenotype:GO:0004622 lysophospholipase activity [PMID:25014274]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:25014274]
Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:23746350]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14690591|PMID:26928762]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14690591]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:14562095]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:21487389]
EXISTENCE:genetic interaction:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:21487389]
EXISTENCE:physical interaction:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:21487389]
Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria; ortholog of human MTBP, which is a DNA replication origin firing factor
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8226802]
EXISTENCE:genetic interaction:GO:0007535 donor selection [PMID:22496671]
EXISTENCE:mutant phenotype:GO:0071470 cellular response to osmotic stress [PMID:24817120]
Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
hypothetical protein; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:3537704]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:26826131]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:9858562|PMID:26826131]
Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; plays an important role in function of eIF5B in stimulating 3' end processing of 18S rRNA in context of 80S ribosomes that have not yet engaged in translation; involved in replication and maintenance of killer double stranded RNA virus
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:12077334]
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:17157260]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:16923967|PMID:17157260]
EXISTENCE:direct assay:GO:1990467 NuA3a histone acetyltransferase complex [PMID:25104842]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:25104842]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:12077334]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:10805724]
EXISTENCE:mutant phenotype:GO:0035064 methylated histone binding [PMID:17157260]
EXISTENCE:physical interaction:GO:0033100 NuA3 histone acetyltransferase complex [PMID:12077334]
Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; shares significant sequence identity with the human candidate tumor suppressor p33-ING1 in C-terminal region
ubiquinol--cytochrome-c reductase catalytic subunit CYT1
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:11279090]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:11279090]
Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19520826]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19520826]
EXISTENCE:genetic interaction:GO:0007089 traversing start control point of mitotic cell cycle [PMID:18160399]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:18160399]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:18160399]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:18160399]
EXISTENCE:physical interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:18160399]
Activator of G1-specific transcription factors MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that arose from the whole genome duplication
dolichyl-P-Glc:Glc1Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase
EXISTENCE:genetic interaction:GO:0006487 protein N-linked glycosylation [PMID:8016100]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:8016100]
EXISTENCE:mutant phenotype:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:3536907]
EXISTENCE:mutant phenotype:GO:0042283 dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity [PMID:3536907]
Glucosyl transferase; involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p; human homolog ALG8 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005768 endosome [PMID:9175702|PMID:9700157]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9175702]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:mutant phenotype:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
EXISTENCE:mutant phenotype:GO:0140312 cargo adaptor activity [PMID:9700157]
EXISTENCE:physical interaction:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0030905 retromer, tubulation complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:9700157]
EXISTENCE:physical interaction:GO:0140312 cargo adaptor activity [PMID:9700157]
Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0000329 fungal-type vacuole membrane [PMID:12748377]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:12748377]
Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10508155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:35134079]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:11359917]
EXISTENCE:direct assay:GO:0016192 vesicle-mediated transport [PMID:11118206]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:10508155]
Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion; interacts with autophagosome component Atg8p
EXISTENCE:genetic interaction:GO:0015888 thiamine transport [PMID:16850348|PMID:9235906]
EXISTENCE:genetic interaction:GO:0034257 nicotinamide riboside transmembrane transporter activity [PMID:18258590]
EXISTENCE:genetic interaction:GO:0034258 nicotinamide riboside transport [PMID:18258590]
EXISTENCE:mutant phenotype:GO:0034257 nicotinamide riboside transmembrane transporter activity [PMID:18258590]
EXISTENCE:mutant phenotype:GO:0034258 nicotinamide riboside transport [PMID:18258590]
EXISTENCE:mutant phenotype:GO:1903088 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport [PMID:24778186]
EXISTENCE:mutant phenotype:GO:1903089 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity [PMID:24778186]
High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; major transporter for 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine) uptake; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity
hypothetical protein; conserved across S. cerevisiae strains; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14730319|PMID:15062096]
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:14730319|PMID:16357219|PMID:28980861]
EXISTENCE:direct assay:GO:0045184 establishment of protein localization [PMID:25236599]
EXISTENCE:direct assay:GO:0051383 kinetochore organization [PMID:22365852]
EXISTENCE:genetic interaction:GO:0000070 mitotic sister chromatid segregation [PMID:15637284]
EXISTENCE:genetic interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:15637284]
EXISTENCE:genetic interaction:GO:0034096 positive regulation of maintenance of meiotic sister chromatid cohesion [PMID:15062096]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:14730319|PMID:15062096|PMID:15637284]
EXISTENCE:mutant phenotype:GO:0000779 condensed chromosome, centromeric region [PMID:28980861]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:15637284]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:17980598|PMID:18081426|PMID:25236599]
EXISTENCE:mutant phenotype:GO:0034090 maintenance of meiotic sister chromatid cohesion [PMID:14752166]
EXISTENCE:mutant phenotype:GO:0034096 positive regulation of maintenance of meiotic sister chromatid cohesion [PMID:15062096]
EXISTENCE:mutant phenotype:GO:0034508 centromere complex assembly [PMID:22365852]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:14730319|PMID:15062096]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:25236599]
EXISTENCE:mutant phenotype:GO:0051383 kinetochore organization [PMID:22365852]
EXISTENCE:mutant phenotype:GO:0051757 meiotic sister chromatid separation [PMID:15062096]
Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
EXISTENCE:direct assay:GO:0004799 thymidylate synthase activity [PMID:6287238]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:8132557]
EXISTENCE:mutant phenotype:GO:0004799 thymidylate synthase activity [PMID:334734]
EXISTENCE:mutant phenotype:GO:0006231 dTMP biosynthetic process [PMID:334734|PMID:794696]
Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature
EXISTENCE:curator inference:GO:0048279 vesicle fusion with endoplasmic reticulum [PMID:8612273]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8612273|PMID:26928762]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9214619]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8612273]
EXISTENCE:mutant phenotype:GO:0016320 endoplasmic reticulum membrane fusion [PMID:9506516]
t-SNARE protein required for retrograde vesicular traffic; involved in Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:26051716]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11532933]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:11532933]
EXISTENCE:genetic interaction:GO:0030163 protein catabolic process [PMID:27481473]
EXISTENCE:genetic interaction:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:11027292]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12769863|PMID:12881429]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:27481473]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:11532933]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:11027292|PMID:11532933]
EXISTENCE:mutant phenotype:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:11910110|PMID:17660569]
GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
Basic zinc-finger protein; similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309|PMID:12150911]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
Component of small ribosomal subunit (SSU) processosome; this complex contains U3 snoRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:8887660]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:25713143]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:25713143]
EXISTENCE:mutant phenotype:GO:0005384 manganese ion transmembrane transporter activity [PMID:8887660]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:8887660]
Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation
DNA-binding SCF ubiquitin ligase subunit DIA2
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16421250]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:19910927]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19858292]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994|PMID:16421250]
EXISTENCE:direct assay:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:19910927]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:19910927]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:11063681]
EXISTENCE:genetic interaction:GO:0032984 protein-containing complex disassembly [PMID:26255844]
EXISTENCE:mutant phenotype:GO:0006275 regulation of DNA replication [PMID:16421250]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:23129771]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:15620357|PMID:25342810]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:22844255]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:19910927]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:22844255]
EXISTENCE:mutant phenotype:GO:0032984 protein-containing complex disassembly [PMID:25342810|PMID:26255844]
Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitination of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:16135509]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:16135509|PMID:24868093|PMID:14562095]
EXISTENCE:direct assay:GO:0006642 triglyceride mobilization [PMID:16135509]
EXISTENCE:direct assay:GO:0042171 lysophosphatidic acid acyltransferase activity [PMID:20016004]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:16135509]
EXISTENCE:mutant phenotype:GO:0006642 triglyceride mobilization [PMID:16135509]
Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:15210111]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15210111|PMID:24470217|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15210111|PMID:14562095]
EXISTENCE:direct assay:GO:0033309 SBF transcription complex [PMID:15210110]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:15210111]
EXISTENCE:genetic interaction:GO:0003714 transcription corepressor activity [PMID:19745812]
EXISTENCE:genetic interaction:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:15210110|PMID:15210111|PMID:19823668]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:19745812]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:26390151]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:15210110|PMID:15210111]
Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; WHI5 expression is repressed in M/early G phase; phosphorylation of Whi5p by G1 cyclin (Cln)/Cdc28p relieves repression and promoter binding by Whi5p, drives nuclear export, and contributes to both the determination of critical cell size at START and cell fate; dephosphorylated and re-imported into the nucleus post-START during the starvation response, reversing commitment
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:24187129]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:12135984]
EXISTENCE:genetic interaction:GO:0019433 triglyceride catabolic process [PMID:24187129]
Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible
dolichyl-diphosphooligosaccharide--protein glycotransferase OST3
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084]
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:23416356]
EXISTENCE:genetic interaction:GO:0006487 protein N-linked glycosylation [PMID:10358084]
EXISTENCE:genetic interaction:GO:0035269 protein O-linked mannosylation [PMID:22492205]
EXISTENCE:genetic interaction:GO:0065003 protein-containing complex assembly [PMID:10358084]
EXISTENCE:mutant phenotype:GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity [PMID:7622558]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:7622558]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:28598353]
EXISTENCE:mutant phenotype:GO:0016020 membrane [PMID:7622558]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:9405463]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins including modification of Pmt2p at N131 and N403
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:14562095]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:15049706]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:22250200]
EXISTENCE:genetic interaction:GO:0055091 phospholipid homeostasis [PMID:35440494]
EXISTENCE:genetic interaction:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:23237950]
EXISTENCE:genetic interaction:GO:0090158 endoplasmic reticulum membrane organization [PMID:23237950]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:33205016]
Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of the Sac1p phosphatase to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER and to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11427713|PMID:11781332]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20035756|PMID:26928762]
EXISTENCE:direct assay:GO:0005227 calcium activated cation channel activity [PMID:1700419]
EXISTENCE:direct assay:GO:0005244 voltage-gated ion channel activity [PMID:1700419]
EXISTENCE:direct assay:GO:0005262 calcium channel activity [PMID:11781332]
EXISTENCE:direct assay:GO:0005267 potassium channel activity [PMID:1700419]
EXISTENCE:direct assay:GO:0005272 sodium channel activity [PMID:1700419]
EXISTENCE:direct assay:GO:0030003 cellular cation homeostasis [PMID:11427713|PMID:11781332]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026036]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:11427713|PMID:11781332]
EXISTENCE:mutant phenotype:GO:0097553 calcium ion transmembrane import into cytosol [PMID:27708136]
Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
ARS1501; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:31754649]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:22748138|PMID:23612966]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:22748138]
EXISTENCE:genetic interaction:GO:0036010 protein localization to endosome [PMID:23264632]
EXISTENCE:genetic interaction:GO:0036258 multivesicular body assembly [PMID:22748138]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0005770 late endosome [PMID:11872141]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:22748138|PMID:23612966]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:10580002]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16643908|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:32398688]
EXISTENCE:mutant phenotype:GO:0004741 [pyruvate dehydrogenase (lipoamide)] phosphatase activity [PMID:18180296]
EXISTENCE:mutant phenotype:GO:0070262 peptidyl-serine dephosphorylation [PMID:18180296]
EXISTENCE:mutant phenotype:GO:1904184 positive regulation of pyruvate dehydrogenase activity [PMID:16643908|PMID:18180296]
Mitochondrial type 2C protein phosphatase (PP2C); involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:21076151]
hypothetical protein that associates with translating ribosomes; interacts with GTPase Rbg1p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:22842922|PMID:26928762]
Non-essential hypothetical protein; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:35766356]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:35766356]
EXISTENCE:mutant phenotype:GO:0046339 diacylglycerol metabolic process [PMID:35766356]
EXISTENCE:mutant phenotype:GO:0097576 vacuole fusion [PMID:35766356]
Protein involved in regulation of diacylglycerol lipids levels; deletion causes sensitivity to unfolded protein response-inducing agents
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8063710]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:17786213]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17786213]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:18208507]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:17425670]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:14668359|PMID:17425670]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:12972567]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:8063710]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:18208507]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:23783029]
EXISTENCE:mutant phenotype:GO:0010513 positive regulation of phosphatidylinositol biosynthetic process [PMID:18208507]
EXISTENCE:mutant phenotype:GO:0036267 invasive filamentous growth [PMID:23783029]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:18397879]
Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
EXISTENCE:mutant phenotype:GO:0004751 ribose-5-phosphate isomerase activity [PMID:8929392]
EXISTENCE:mutant phenotype:GO:0006098 pentose-phosphate shunt [PMID:8929392]
EXISTENCE:mutant phenotype:GO:0008615 pyridoxine biosynthetic process [PMID:14690456]
Ribose-5-phosphate ketol-isomerase; catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:genetic interaction:GO:0042254 ribosome biogenesis [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication
hypothetical protein; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:11046143|PMID:2190694]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:10684247]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:16760982|PMID:20098417]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:7929578]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:11046143|PMID:16118201]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:12411502|PMID:16118201|PMID:7929578]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:10525531|PMID:12917401|PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
EXISTENCE:direct assay:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:9020857]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8631921]
EXISTENCE:genetic interaction:GO:0006487 protein N-linked glycosylation [PMID:9182588]
EXISTENCE:genetic interaction:GO:0006493 protein O-linked glycosylation [PMID:9182588]
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:9182588]
Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005476 carnitine:acyl carnitine antiporter activity [PMID:10545096]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10545096]
EXISTENCE:direct assay:GO:0006631 fatty acid metabolic process [PMID:10545096]
Mitochondrial inner membrane carnitine transporter; required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation; human homolog SLC25A20 complements yeast null mutant
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:14674766]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20162532|PMID:3513173]
EXISTENCE:direct assay:GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway [PMID:3891097]
EXISTENCE:genetic interaction:GO:0045762 positive regulation of adenylate cyclase activity [PMID:2981630|PMID:3891097]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:12782684]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:6327067]
GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in regulation of cell proliferation; localized to the plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
dolichyl-diphosphooligosaccharide-protein glycotransferase
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:7593165]
Non-catalytic epsilon subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER; forms a pocket with Wbp1p involved in binding terminal glucose units of the donor glycan; located in the ER lumen
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:24656818]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:24656818]
Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated
hypothetical protein; expressed at both mRNA and protein levels
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:9202390|PMID:26928762]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:17054985]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9425154]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:9685264]
EXISTENCE:genetic interaction:GO:0048278 vesicle docking [PMID:9245783]
EXISTENCE:genetic interaction:GO:0051469 vesicle fusion with vacuole [PMID:9245783]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0097352 autophagosome maturation [PMID:9202390]
Syntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10523302]
EXISTENCE:mutant phenotype:GO:0007188 adenylate cyclase-modulating G protein-coupled receptor signaling pathway [PMID:10523302]
Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
EXISTENCE:direct assay:GO:0003852 2-isopropylmalate synthase activity [PMID:11158296|PMID:25841022]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11158296|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0003852 2-isopropylmalate synthase activity [PMID:10790691]
EXISTENCE:genetic interaction:GO:0009098 leucine biosynthetic process [PMID:10790691]
EXISTENCE:mutant phenotype:GO:0009098 leucine biosynthetic process [PMID:25841022]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:25841022]
Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53
EXISTENCE:direct assay:GO:0004438 phosphatidylinositol-3-phosphate phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11094088]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9560389]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:11094088]
EXISTENCE:direct assay:GO:0043812 phosphatidylinositol-4-phosphate phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0043813 phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10224048]
EXISTENCE:mutant phenotype:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:9438131]
EXISTENCE:mutant phenotype:GO:0046856 phosphatidylinositol dephosphorylation [PMID:9438131]
Polyphosphatidylinositol phosphatase; dephosphorylates multiple phosphatidylinositol phosphates; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; INP53 has a paralog, INP52, that arose from the whole genome duplication
transcription factor TFIIIC subunit TFC7
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:1474578|PMID:2002052|PMID:9584160]
EXISTENCE:direct assay:GO:0000995 RNA polymerase III general transcription initiation factor activity [PMID:9584160]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:3633923|PMID:8464480|PMID:2183033|PMID:9584160]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2002052]
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:23569204]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:mutant phenotype:GO:0000995 RNA polymerase III general transcription initiation factor activity [PMID:9584160]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:9584160]
RNA pol III transcription initiation factor complex (TFIIIC) subunit; part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; TFC7 has a paralog, YNL108C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:17203074]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:17203074]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17203074|PMID:14562095]
EXISTENCE:genetic interaction:GO:0006409 tRNA export from nucleus [PMID:17203074|PMID:22008473]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:17203074]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:33753324]
EXISTENCE:physical interaction:GO:0005643 nuclear pore [PMID:17203074]
EXISTENCE:physical interaction:GO:0006409 tRNA export from nucleus [PMID:17203074]
Component of COPI-mediated trafficking and tRNA nuclear export; enables Rna1p to access and activate Gsp1p-GTP bound to the export receptor tRNA complex during aminoacylation-dependent tRNA export; copurifies with tRNA export receptors; interacts with COPI coat proteins and regulates Golgi-to-ER trafficking; membrane fraction associated; mutations in members of the homologous human SCY1-like (SCYL) family of pseudokinases are linked to peripheral neuropathy, cerebellar atrophy, ataxia and ALS
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11839825|PMID:16467472]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11839825]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:9799362|PMID:16467472]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11839825]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:16467472]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11839825]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11839825]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:16467472]
EXISTENCE:mutant phenotype:GO:0071322 cellular response to carbohydrate stimulus [PMID:11839825|PMID:16467472]
Zinc-finger transcription factor; involved in the diauxic shift; activates transcription of genes involved in carbon metabolism and energy production on glucose, while it activates transcription of genes involved in cell wall organization and biogenesis on glycerol-lactate; may also function as a co-repressor; capable of forming the prion [AZF1+]; relocalizes to the cytosol in response to hypoxia; contains polyglutamine and polyasparagine domains
Putative mitochondrial hypothetical protein; null mutant is viable
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:27672095]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:19843283]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:19843283]
EXISTENCE:direct assay:GO:1990070 TRAPPI protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990072 TRAPPIII protein complex [PMID:20375281]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9564032]
EXISTENCE:genetic interaction:GO:0016239 positive regulation of macroautophagy [PMID:27672095]
EXISTENCE:genetic interaction:GO:0031503 protein-containing complex localization [PMID:27672095]
EXISTENCE:genetic interaction:GO:0034497 protein localization to phagophore assembly site [PMID:27672095]
EXISTENCE:genetic interaction:GO:0065003 protein-containing complex assembly [PMID:19843283]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:27672095]
Core component of TRAPP complexes I, II and IV; transport protein particle (TRAPP) complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII, and IV); proposed subunit of a novel complex, TRAPPIV, that may function redundantly with TRAPPIII as a GEF that activates Ypt1 during autophagy
DNA-directed RNA polymerase III core subunit RPO31
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:3905793|PMID:10611227]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
proteasome regulatory particle base subunit RPT5
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:9724628]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:19843524]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19412160]
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:30011030]
EXISTENCE:direct assay:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12612080|PMID:11483523]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:30011030]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:30011030]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:11972772|PMID:25851096]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12612080|PMID:30011030]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11483523]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:30011030]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:30011030]
EXISTENCE:direct assay:GO:0007096 regulation of exit from mitosis [PMID:11972772]
EXISTENCE:direct assay:GO:0030874 nucleolar chromatin [PMID:25851096]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:25851096]
EXISTENCE:mutant phenotype:GO:2000234 positive regulation of rRNA processing [PMID:25851096]
Serine kinase involved in cell cycle regulation and rDNA integrity; associated with late pre-40S particles via its conserved C-terminal domain; prevents premature use of pre-40S particles in translation; association with pre-40S particles regulated by its catalytic ATPase site and likely occurs after the release of Rio2p from these particles; involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA; phosphorylates Rpa43p in anaphase to remove Pol I from rDNA
EXISTENCE:direct assay:GO:0004032 alditol:NADP+ 1-oxidoreductase activity [PMID:11306085]
EXISTENCE:direct assay:GO:0004033 aldo-keto reductase (NADP) activity [PMID:17140678|PMID:10818358]
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:17962934]
EXISTENCE:direct assay:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:direct assay:GO:0042843 D-xylose catabolic process [PMID:12271459]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:17919749]
EXISTENCE:genetic interaction:GO:0061610 glycerol to glycerone phosphate metabolic process [PMID:22979944]
EXISTENCE:mutant phenotype:GO:0061610 glycerol to glycerone phosphate metabolic process [PMID:22979944]
EXISTENCE:mutant phenotype:GO:1990042 glycerol dehydrogenase [NAD(P)+] activity [PMID:22979944]
Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; human homolog AKR1B1 can complement yeast null mutant; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003785 actin monomer binding [PMID:10953013]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:8247001]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:7592954]
EXISTENCE:direct assay:GO:0042989 sequestering of actin monomers [PMID:10953013]
EXISTENCE:direct assay:GO:0070064 proline-rich region binding [PMID:8247001|PMID:9698363]
EXISTENCE:direct assay:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:12134165]
EXISTENCE:genetic interaction:GO:0046907 intracellular transport [PMID:9720869]
EXISTENCE:mutant phenotype:GO:0003785 actin monomer binding [PMID:10953013]
EXISTENCE:mutant phenotype:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:8247001]
EXISTENCE:mutant phenotype:GO:0042989 sequestering of actin monomers [PMID:10953013]
EXISTENCE:mutant phenotype:GO:0070064 proline-rich region binding [PMID:8247001]
EXISTENCE:mutant phenotype:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:12134165]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:12419188]
Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:20732871]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15643076]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:20732871]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:14710186]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:11927560|PMID:11884586]
Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:1429680]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:1429680|PMID:24069405]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:15933713|PMID:24069405]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:24069405]
EXISTENCE:mutant phenotype:GO:0010636 positive regulation of mitochondrial fusion [PMID:23317502]
EXISTENCE:mutant phenotype:GO:0010992 ubiquitin recycling [PMID:25622294]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:24069405|PMID:23317502]
EXISTENCE:mutant phenotype:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:19165343]
Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; controls K63 homeostasis during oxidative stress; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9452453]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:9452453]
Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
EXISTENCE:direct assay:GO:0006083 acetate metabolic process [PMID:10855721]
EXISTENCE:direct assay:GO:0016788 hydrolase activity, acting on ester bonds [PMID:10855721]
Isoamyl acetate-hydrolyzing esterase; required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12455995|PMID:17981141|PMID:18166650|PMID:7498791]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:29074565]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:22842922]
EXISTENCE:direct assay:GO:0032177 cellular bud neck split septin rings [PMID:18166650]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
EXISTENCE:direct assay:GO:0071597 cellular birth scar [PMID:29074565]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:7498791]
EXISTENCE:genetic interaction:GO:0005096 GTPase activator activity [PMID:7498791]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:14517318]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:8657111]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:18166650]
EXISTENCE:physical interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:7498791]
EXISTENCE:physical interaction:GO:0007118 budding cell apical bud growth [PMID:7498791]
EXISTENCE:physical interaction:GO:0007119 budding cell isotropic bud growth [PMID:7498791]
EXISTENCE:physical interaction:GO:2000222 positive regulation of pseudohyphal growth [PMID:7498791]
GTPase-activating protein for polarity-regulator Cdc42p (RhoGAP); required for proper bud site selection; transiently localizes to previous cell division sites (bud scars; cytokinesis remnants), interacting with Nis1p and Nba1p, to prevent Cdc42p repolarization; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from the bud neck to cytoplasm upon DNA replication stress; contains N-terminal LIM domains and a C-terminal GAP domain
phosphoribosylaminoimidazole carboxylase ADE2
EXISTENCE:mutant phenotype:GO:0004638 phosphoribosylaminoimidazole carboxylase activity [PMID:5767024]
EXISTENCE:mutant phenotype:GO:0006144 purine nucleobase metabolic process [PMID:8939809]
Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
ARS1516; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18397879]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:18397879]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:18397879]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:18397879]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:14515169]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:14515169|PMID:18397879]
Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
EXISTENCE:direct assay:GO:0000064 L-ornithine transmembrane transporter activity [PMID:9237680]
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:8798783]
EXISTENCE:direct assay:GO:1990575 mitochondrial L-ornithine transmembrane transport [PMID:9237680]
EXISTENCE:mutant phenotype:GO:0006526 arginine biosynthetic process [PMID:8798783]
Ornithine transporter of the mitochondrial inner membrane; exports ornithine from mitochondria as part of arginine biosynthesis; functionally complemented by human ortholog, SLC25A15, which is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome, but HHH-associated variants fail to complement
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
putative haloacid dehalogenase-like hydrolase
EXISTENCE:direct assay:GO:0033883 pyridoxal phosphatase activity [PMID:26071590]
Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0140318 protein transporter activity [PMID:9700157]
EXISTENCE:physical interaction:GO:0005768 endosome [PMID:9700157]
EXISTENCE:physical interaction:GO:0140318 protein transporter activity [PMID:9700157]
Subunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16950777]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:10835368|PMID:16950777]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:30876804]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:16950777]
EXISTENCE:mutant phenotype:GO:0045901 positive regulation of translational elongation [PMID:19338753]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:16950777]
Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12207708|PMID:11591390]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:11591390|PMID:12207708]
EXISTENCE:genetic interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:12207708]
Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004449 isocitrate dehydrogenase (NAD+) activity [PMID:16884682]
EXISTENCE:direct assay:GO:0005962 mitochondrial isocitrate dehydrogenase complex (NAD+) [PMID:16884682]
EXISTENCE:direct assay:GO:0006102 isocitrate metabolic process [PMID:16884682]
Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes oxidation of isocitrate to alpha-ketoglutarate in TCA cycle; targeted to vacuole via AP-3 pathway
EXISTENCE:mutant phenotype:GO:1902600 proton transmembrane transport [PMID:9450541]
Protein of unassigned function; involved in activation of the Pma1p plasma membrane H+-ATPase by glucose; contains peptide signal for membrane localization
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:24069405]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:15933713]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15933713]
Protein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11399075|PMID:12024012|PMID:9755175]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12024012]
EXISTENCE:direct assay:GO:0010508 positive regulation of autophagy [PMID:26649943]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:27336723]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26649943]
EXISTENCE:direct assay:GO:2001159 regulation of protein localization by the Cvt pathway [PMID:26649943]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9811878]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12024012|PMID:15466424|PMID:9811878]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:27336723|PMID:17594096]
EXISTENCE:physical interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9755175|PMID:11399075]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11399075]
Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; has direct role in INO1 transcriptional memory; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C)
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11011149]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:23644470]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:17320816]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:17320816]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:10952318|PMID:24034245]
Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; has mRNA binding activity
succinate--CoA ligase (GDP-forming) subunit alpha
EXISTENCE:direct assay:GO:0004775 succinate-CoA ligase (ADP-forming) activity [PMID:9874242]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9874242|PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0006104 succinyl-CoA metabolic process [PMID:9874242]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0004775 succinate-CoA ligase (ADP-forming) activity [PMID:9874242]
Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
YORWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
ARS1502; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004788 thiamine diphosphokinase activity [PMID:18652651|PMID:8394343]
EXISTENCE:mutant phenotype:GO:0004788 thiamine diphosphokinase activity [PMID:8394343]
EXISTENCE:mutant phenotype:GO:0009229 thiamine diphosphate biosynthetic process [PMID:8394343]
Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18482875]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:19430531]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:17170004]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:12912927]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:19430531]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:0090618 DNA clamp unloading [PMID:29440488|PMID:23499004]
EXISTENCE:physical interaction:GO:0031391 Elg1 RFC-like complex [PMID:12909721]
Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12502737]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10923024]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:18755838]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:12502737]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:18755838]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12736301]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12736301]
EXISTENCE:mutant phenotype:GO:0042255 ribosome assembly [PMID:18755838]
Essential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15631992]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:15631992]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:16020778]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266|PMID:16020778]
Mitochondrial inner membrane protein with similarity to Mdm31p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; variation between SK1 and S288C at residues 182 and 262 impacts invasive growth and mitochondrial network structure
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:25561498]
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:7493316]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:25561498]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:7493316]
Essential protein that promotes the first step of splicing; required for the final stages of spliceosome maturation and activation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction; stimulates Prp2p ATPase activity
glycosylphosphatidylinositol-alpha 1,2 mannosyltransferase
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:11356840]
EXISTENCE:mutant phenotype:GO:0006276 plasmid maintenance [PMID:2005867]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:11356840]
Alpha 1,2-mannosyltransferase; involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
mitochondrial 54S ribosomal protein YmL23
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23509072]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:9151978|PMID:12392552]
Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
DNA-directed RNA polymerase II core subunit RPB2
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:26945063]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:2406558]
RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
ARS1518; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11935221]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:26040717]
EXISTENCE:genetic interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:26040717]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:26040717]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:26040717]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26040717]
Autophagy receptor with a role in endoplasmic reticulum degradation; involved specifically in autophagy of cortical and cytoplasmic ER in response to nitrogen starvation or rapamycin treatment; acts with COPII subunit Sfb3p; localizes to the cortical and cytoplasmic ER; similar to human FAM134B, which is also involved in ER autophagy and is associated with sensory neuropathy
ATP-binding cassette multidrug transporter PDR5
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:8810273]
EXISTENCE:direct assay:GO:0008559 ABC-type xenobiotic transporter activity [PMID:21207971]
EXISTENCE:genetic interaction:GO:0030003 cellular cation homeostasis [PMID:8985171]
EXISTENCE:mutant phenotype:GO:0008559 ABC-type xenobiotic transporter activity [PMID:15707993]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:17881724]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:8985171]
EXISTENCE:mutant phenotype:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:9450972]
Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:23275891]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Glycosylated integral ER membrane hypothetical protein; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
EXISTENCE:direct assay:GO:0050483 IMP 5'-nucleotidase activity [PMID:12735798]
EXISTENCE:mutant phenotype:GO:0006190 inosine salvage [PMID:12735798]
Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase; catalyzes the breakdown of IMP to inosine; responsible for production of nicotinamide riboside and nicotinic acid riboside; expression positively regulated by nicotinic acid and glucose availability; does not show similarity to known 5'-nucleotidases from other organisms
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16204216]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:23122649]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:12761287|PMID:23443663]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:12761287]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:26960795]
EXISTENCE:genetic interaction:GO:1990683 DNA double-strand break attachment to nuclear envelope [PMID:27056668]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:23122649]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16204216]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:22345352|PMID:23122649]
EXISTENCE:mutant phenotype:GO:0019789 SUMO transferase activity [PMID:23122649]
SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:9087403]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18504300|PMID:26928762]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:mutant phenotype:GO:0004175 endopeptidase activity [PMID:9207060|PMID:9312134]
Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
mitochondrial 37S ribosomal protein PET123
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9012791|PMID:9630245]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:8918241]
EXISTENCE:physical interaction:GO:1990935 splicing factor binding [PMID:9528767]
Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9545233]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9412460|PMID:22842922]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:9545233|PMID:9412460]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:9545233]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0006403 RNA localization [PMID:12167699]
EXISTENCE:mutant phenotype:GO:0006404 RNA import into nucleus [PMID:16040803|PMID:17409072]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:9412460|PMID:9545233]
EXISTENCE:mutant phenotype:GO:0035719 tRNA import into nucleus [PMID:33074312]
EXISTENCE:mutant phenotype:GO:0051031 tRNA transport [PMID:17409072|PMID:20032305]
EXISTENCE:mutant phenotype:GO:0061015 snRNA import into nucleus [PMID:31189105]
Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
hypothetical protein; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10052137]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11909958]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:10052137|PMID:11134057]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9767597]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11134057]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9767597]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:10052137]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9767597]
Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000298 endopolyphosphatase activity [PMID:21775424|PMID:31175919]
EXISTENCE:direct assay:GO:0006798 polyphosphate catabolic process [PMID:21775424]
EXISTENCE:direct assay:GO:0008486 diphosphoinositol-polyphosphate diphosphatase activity [PMID:10419486]
EXISTENCE:direct assay:GO:0015961 diadenosine polyphosphate catabolic process [PMID:10085096|PMID:9450008]
EXISTENCE:direct assay:GO:0034431 bis(5'-adenosyl)-hexaphosphatase activity [PMID:10085096|PMID:9450008]
EXISTENCE:direct assay:GO:0034432 bis(5'-adenosyl)-pentaphosphatase activity [PMID:10085096|PMID:9450008]
EXISTENCE:direct assay:GO:0050072 m7G(5')pppN diphosphatase activity [PMID:23353937]
EXISTENCE:direct assay:GO:1990174 phosphodiesterase decapping endonuclease activity [PMID:23353937]
Diadenosine and diphosphoinositol polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:19665976|PMID:26928762]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0016320 endoplasmic reticulum membrane fusion [PMID:22508509]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:19665976]
EXISTENCE:genetic interaction:GO:0007029 endoplasmic reticulum organization [PMID:22508509|PMID:19665976]
EXISTENCE:genetic interaction:GO:0016320 endoplasmic reticulum membrane fusion [PMID:22508509]
EXISTENCE:genetic interaction:GO:0061024 membrane organization [PMID:12427979]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:19665976]
EXISTENCE:mutant phenotype:GO:0016320 endoplasmic reticulum membrane fusion [PMID:22508509]
Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:19127978]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17030511|PMID:19127978]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19127978]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17030511]
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:19127978]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17030511]
EXISTENCE:mutant phenotype:GO:0071032 nuclear mRNA surveillance of mRNP export [PMID:19127978]
EXISTENCE:physical interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17030511]
RNA endoribonuclease involved in perinuclear mRNP quality control; involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:3533916]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916]
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:24492965]
Protein component of the small (40S) ribosomal subunit; has an extraribosomal function in regulation of RPS28B, in which Rps28Ap binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28A has a paralog, RPS28B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004819 glutamine-tRNA ligase activity [PMID:2991203]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
EXISTENCE:mutant phenotype:GO:0006425 glutaminyl-tRNA aminoacylation [PMID:2991203]
Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12493772|PMID:16141212]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12459470]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16141212]
EXISTENCE:direct assay:GO:0006665 sphingolipid metabolic process [PMID:9677363]
EXISTENCE:direct assay:GO:0017050 D-erythro-sphingosine kinase activity [PMID:9677363]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:17686782]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:11278643]
Sphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:14512416]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:14512416]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:14512416]
Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
ARS1519; Autonomously Replicating Sequence
5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA) diphosphatase
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:16963086]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:16963086]
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:16963086]
EXISTENCE:genetic interaction:GO:0009267 cellular response to starvation [PMID:15240832]
m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:23447536]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:9420330]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17890783|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0008374 O-acyltransferase activity [PMID:17890783]
EXISTENCE:genetic interaction:GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity [PMID:17675291]
EXISTENCE:genetic interaction:GO:0046474 glycerophospholipid biosynthetic process [PMID:17675291]
EXISTENCE:genetic interaction:GO:0090640 phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine [PMID:30514764]
EXISTENCE:mutant phenotype:GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity [PMID:17675291|PMID:17726007]
EXISTENCE:mutant phenotype:GO:0008374 O-acyltransferase activity [PMID:17890783]
EXISTENCE:mutant phenotype:GO:0046474 glycerophospholipid biosynthetic process [PMID:17890783|PMID:17675291|PMID:17726007]
EXISTENCE:mutant phenotype:GO:0047184 1-acylglycerophosphocholine O-acyltransferase activity [PMID:17726007|PMID:17951629]
Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
EXISTENCE:direct assay:GO:0004325 ferrochelatase activity [PMID:1304908]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7629135]
EXISTENCE:mutant phenotype:GO:0004325 ferrochelatase activity [PMID:2185242]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:7035824]
Ferrochelatase; a mitochondrial inner membrane protein, catalyzes insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; human homolog FECH can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:mutant phenotype:GO:0048278 vesicle docking [PMID:20826607]
Component of the meiotic outer plaque; a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0000164 protein phosphatase type 1 complex [PMID:8289829]
EXISTENCE:direct assay:GO:0031072 heat shock protein binding [PMID:10207049]
EXISTENCE:genetic interaction:GO:0019888 protein phosphatase regulator activity [PMID:1310938]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:10072380]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10207049]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:11459182]
EXISTENCE:physical interaction:GO:0005977 glycogen metabolic process [PMID:8289829]
EXISTENCE:physical interaction:GO:0051321 meiotic cell cycle [PMID:8754819]
Regulatory subunit for Glc7p type-1 protein phosphatase (PP1); tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; GAC1 has a paralog, PIG1, that arose from the whole genome duplication
cleavage polyadenylation factor subunit SYC1
EXISTENCE:curator inference:GO:0003723 RNA binding [PMID:12819204]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:mutant phenotype:GO:0031441 negative regulation of mRNA 3'-end processing [PMID:16431986]
Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity [PMID:10455114]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:10381339]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:10381339]
Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed; DCI1 has a paralog, ECI1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:10512884|PMID:23290554]
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:9214384]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10512884|PMID:9024694|PMID:16824951]
EXISTENCE:direct assay:GO:0032233 positive regulation of actin filament bundle assembly [PMID:19158382]
EXISTENCE:direct assay:GO:0045010 actin nucleation [PMID:23290554]
EXISTENCE:direct assay:GO:0071933 Arp2/3 complex binding [PMID:10322115]
EXISTENCE:direct assay:GO:0140224 SLAC complex [PMID:22973053]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:10322115|PMID:11604421|PMID:12499356|PMID:12814545|P MID:16824951|PMID:22973053]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:mutant phenotype:GO:0003779 actin binding [PMID:27637067]
EXISTENCE:mutant phenotype:GO:0030041 actin filament polymerization [PMID:27637067]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:physical interaction:GO:0003779 actin binding [PMID:23290554]
EXISTENCE:physical interaction:GO:0140224 SLAC complex [PMID:22973053]
Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30B has a paralog, RPS30A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
hypothetical protein; required for survival upon exposure to K1 killer toxin
O-phospho-L-serine:2-oxoglutarate transaminase
EXISTENCE:mutant phenotype:GO:0004648 O-phospho-L-serine:2-oxoglutarate aminotransferase activity [PMID:8017107]
EXISTENCE:mutant phenotype:GO:0006564 L-serine biosynthetic process [PMID:8017107]
EXISTENCE:mutant phenotype:GO:0009070 serine family amino acid biosynthetic process [PMID:1326413]
EXISTENCE:mutant phenotype:GO:0009113 purine nucleobase biosynthetic process [PMID:10509016]
3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9182768]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9182768]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9182768]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at position U1187
EXISTENCE:genetic interaction:GO:0006997 nucleus organization [PMID:8455603]
GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication
hypothetical protein; proper regulation of expression during heat stress is sphingolipid-dependent; mCherry fusion protein localizes to the vacuole; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003746 translation elongation factor activity [PMID:3301847]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:3301847]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:23727608|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0032543 mitochondrial translation [PMID:6353412]
EXISTENCE:genetic interaction:GO:0032543 mitochondrial translation [PMID:15695360|PMID:16777356]
EXISTENCE:mutant phenotype:GO:0070125 mitochondrial translational elongation [PMID:3905388]
Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:10679030]
hypothetical protein; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:17693258]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:17693258]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Component of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
EXISTENCE:direct assay:GO:0004338 glucan exo-1,3-beta-glucosidase activity [PMID:7875558]
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:19779569]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0009277 fungal-type cell wall [PMID:8419289]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:8419289]
Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance; SPR1 has a paralog, EXG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:26906737]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18603780|PMID:26906737|PMID:22842922]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:18603780]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:9271422]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:9271422]
EXISTENCE:mutant phenotype:GO:2001033 negative regulation of double-strand break repair via nonhomologous end joining [PMID:23417015]
EXISTENCE:physical interaction:GO:0032183 SUMO binding [PMID:17728242]
Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:genetic interaction:GO:1903088 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport [PMID:24778186]
EXISTENCE:genetic interaction:GO:1903089 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity [PMID:24778186]
Transporter of thiamine or related compound; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); shares sequence similarity with Thi7p
YORCTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:curator inference:GO:0006413 translational initiation [PMID:9023104]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0071074 eukaryotic initiation factor eIF2 binding [PMID:14698289]
Methionine initiator tRNA (tRNA-Met); predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:14517321]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:14517321]
EXISTENCE:physical interaction:GO:0007031 peroxisome organization [PMID:14517321]
Peripheral peroxisomal membrane protein; involved in controlling peroxisome size and number, interacts with Pex25p; PEX27 has a paralog, PEX25, that arose from the whole genome duplication
transcription initiation factor IIA large subunit
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:10567590]
EXISTENCE:direct assay:GO:0005672 transcription factor TFIIA complex [PMID:7862117]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:7862117]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:7604282|PMID:10567590]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10567590]
EXISTENCE:genetic interaction:GO:0003712 transcription coregulator activity [PMID:10567590]
EXISTENCE:genetic interaction:GO:0017025 TBP-class protein binding [PMID:10567590]
EXISTENCE:genetic interaction:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10567590]
TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa2p, for ribosomal protein gene transcription in vivo; homologous to largest and second largest subunits of human and Drosophila TFIIA
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:10427094|PMID:15282802]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:11533655]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:10427094]
EXISTENCE:direct assay:GO:0071459 protein localization to chromosome, centromeric region [PMID:30903360]
EXISTENCE:direct assay:GO:0098653 centromere clustering [PMID:30903360]
EXISTENCE:genetic interaction:GO:0031536 positive regulation of exit from mitosis [PMID:14551257]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:11533655|PMID:10427094]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16443750]
EXISTENCE:mutant phenotype:GO:0007097 nuclear migration [PMID:16443750]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:14551257|PMID:27418100]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:10835382]
EXISTENCE:mutant phenotype:GO:0098653 centromere clustering [PMID:23283988]
Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
EXISTENCE:mutant phenotype:GO:0009249 protein lipoylation [PMID:19570983]
Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
EXISTENCE:direct assay:GO:0004198 calcium-dependent cysteine-type endopeptidase activity [PMID:29363267|PMID:22761449]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12062425]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:24855027]
EXISTENCE:direct assay:GO:0006915 apoptotic process [PMID:11983181]
EXISTENCE:mutant phenotype:GO:0004198 calcium-dependent cysteine-type endopeptidase activity [PMID:22761449]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:20624963]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:16170310|PMID:16301538]
Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11410665]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23222640]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:11410665]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:11410665]
EXISTENCE:direct assay:GO:1990904 ribonucleoprotein complex [PMID:11410665]
EXISTENCE:mutant phenotype:GO:0003729 mRNA binding [PMID:31455610]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:31455610]
EXISTENCE:mutant phenotype:GO:0007088 regulation of mitotic nuclear division [PMID:8913743]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:24424022]
EXISTENCE:mutant phenotype:GO:0035269 protein O-linked mannosylation [PMID:31835530]
EXISTENCE:mutant phenotype:GO:0045048 protein insertion into ER membrane [PMID:31455610]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8913743]
Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
EXISTENCE:direct assay:GO:0000154 rRNA modification [PMID:8643404]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8643404]
EXISTENCE:direct assay:GO:0008989 rRNA (guanine-N1-)-methyltransferase activity [PMID:8643404]
Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
imidazoleglycerol-phosphate dehydratase HIS3
EXISTENCE:direct assay:GO:0000105 histidine biosynthetic process [PMID:341150]
EXISTENCE:direct assay:GO:0004424 imidazoleglycerol-phosphate dehydratase activity [PMID:341150]
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241]
EXISTENCE:mutant phenotype:GO:0004424 imidazoleglycerol-phosphate dehydratase activity [PMID:14190241]
Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
DEAD-box ATP-dependent RNA helicase DED1
EXISTENCE:direct assay:GO:0000390 spliceosomal complex disassembly [PMID:16556937]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:10364207|PMID:16216083]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:30281017|PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25013175]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25013175]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405|PMID:21925384]
EXISTENCE:direct assay:GO:0033592 RNA strand annealing activity [PMID:16216083]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:10364207]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:14763975|PMID:9045610]
EXISTENCE:mutant phenotype:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:30281017]
EXISTENCE:physical interaction:GO:0031370 eukaryotic initiation factor 4G binding [PMID:21925384]
ATP-dependent DEAD-box RNA helicase with strand-annealing activity; promotes eIF4F-dependent 48S translation preinitiation complex (PIC) assembly, stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with Dbp1p in PIC attachment and scanning; ATPase activity stimulated by mRNA cap-associated factor binding; directly binds eIF4G; role in spliceosomal complex disassembly; mutation in human homolog DBY associated with male infertility; human homolog DDX3X complements the null
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22621929]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:22621929]
Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
mRNA-binding ribosome synthesis protein NOC2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:11371346]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:11371346]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23209026]
EXISTENCE:direct assay:GO:0030690 Noc1p-Noc2p complex [PMID:11371346]
EXISTENCE:direct assay:GO:0030691 Noc2p-Noc3p complex [PMID:11371346]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:23209026|PMID:11371346]
Protein involved in ribosome biogenesis; forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes; forms a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit
ARS1521; Autonomously Replicating Sequence
DNA-directed RNA polymerase III core subunit RET1
EXISTENCE:genetic interaction:GO:0042797 tRNA transcription by RNA polymerase III [PMID:2199311]
Second-largest subunit of RNA polymerase III; RNA polymerase III is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:10523653]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10523653]
EXISTENCE:genetic interaction:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:9224718]
EXISTENCE:genetic interaction:GO:0035335 peptidyl-tyrosine dephosphorylation [PMID:9224718|PMID:10523653]
EXISTENCE:genetic interaction:GO:0043937 regulation of sporulation [PMID:10679022]
EXISTENCE:genetic interaction:GO:0071507 pheromone response MAPK cascade [PMID:9224718]
EXISTENCE:genetic interaction:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:10523653]
EXISTENCE:genetic interaction:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:10523653]
EXISTENCE:mutant phenotype:GO:0035335 peptidyl-tyrosine dephosphorylation [PMID:10523653]
EXISTENCE:mutant phenotype:GO:0051457 maintenance of protein location in nucleus [PMID:10817757]
EXISTENCE:mutant phenotype:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:10523653]
EXISTENCE:mutant phenotype:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:10523653]
Phosphotyrosine-specific phosphatase; major role in osmolarity sensing through dephosphorylation of the Hog1p MAPK with a minor role by Ptp3p; inactivates and regulates Hog1p localization; major role in the cell wall integrity pathway through dephosphorylation of MAPK Slt2p with a minor role by Ptp3p; minor role with Msg5p in the pheromone adaptive response through dephosphorylation of MAPK Fus3p with major role by Ptp3p; co-regulates the calcium signaling pathway with Msg5p; nuclear localized
EXISTENCE:direct assay:GO:0004516 nicotinate phosphoribosyltransferase activity [PMID:9521740]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11884393]
EXISTENCE:direct assay:GO:0019358 nicotinate nucleotide salvage [PMID:11884393]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:11901108]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11901108]
Nicotinate phosphoribosyltransferase; acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
DNA-directed RNA polymerase core subunit RPB10
EXISTENCE:curator inference:GO:0006383 transcription by RNA polymerase III [PMID:1748681]
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:1748681]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:1748681|PMID:3905793]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:9837969|PMID:1748681]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:1748681]
EXISTENCE:genetic interaction:GO:0006360 transcription by RNA polymerase I [PMID:10085073]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:10085073]
RNA polymerase subunit ABC10-beta; common to RNA polymerases I, II, and III
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:19236101]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:19236101]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:27849155]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:19505460]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:11038181]
EXISTENCE:direct assay:GO:0030061 mitochondrial crista [PMID:19505460]
EXISTENCE:direct assay:GO:0061025 membrane fusion [PMID:22977249]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:19236101]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:19236101]
EXISTENCE:direct assay:GO:0097002 mitochondrial inner boundary membrane [PMID:19505460|PMID:27849155]
EXISTENCE:direct assay:GO:1901612 cardiolipin binding [PMID:19236101]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:1532158]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:15125685]
EXISTENCE:mutant phenotype:GO:0008053 mitochondrial fusion [PMID:12808034|PMID:17055438]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:32265272]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:27849155]
Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; promotes membrane bending; plays a direct role in formation and maintenance of lamellar, but not of tubular, cristae; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy
EXISTENCE:direct assay:GO:0005834 heterotrimeric G-protein complex [PMID:8995254]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8995254]
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:11073983]
EXISTENCE:direct assay:GO:0019901 protein kinase binding [PMID:9428767]
EXISTENCE:direct assay:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8417317|PMID:8995254]
EXISTENCE:direct assay:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:2107073]
EXISTENCE:genetic interaction:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8417317]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:2107073]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:15192700]
EXISTENCE:mutant phenotype:GO:0005834 heterotrimeric G-protein complex [PMID:2105453]
EXISTENCE:mutant phenotype:GO:0010525 regulation of transposition, RNA-mediated [PMID:9566871]
EXISTENCE:mutant phenotype:GO:0031267 small GTPase binding [PMID:12660244]
EXISTENCE:mutant phenotype:GO:0043577 chemotropism [PMID:23613469|PMID:10087263]
EXISTENCE:mutant phenotype:GO:0061951 establishment of protein localization to plasma membrane [PMID:9732267]
EXISTENCE:mutant phenotype:GO:1903260 protein localization to mating projection tip [PMID:12660244]
EXISTENCE:physical interaction:GO:0001965 G-protein alpha-subunit binding [PMID:8417317]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:8649369]
EXISTENCE:physical interaction:GO:0019901 protein kinase binding [PMID:9428767]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:12660244]
EXISTENCE:physical interaction:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8649369]
EXISTENCE:physical interaction:GO:0031682 G-protein gamma-subunit binding [PMID:8417317|PMID:8649369]
EXISTENCE:physical interaction:GO:0097110 scaffold protein binding [PMID:7667635]
EXISTENCE:physical interaction:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
G protein beta subunit; forms a dimer with Ste18p to activate mating signaling pathway, forms heterotrimer with Gpa1p and Ste18p to dampen signaling; pheromone-induced phosphorylation plays critical role in chemotropism; may recruit Rho1p to polarized growth site during mating; contains WD40 repeats
EXISTENCE:curator inference:GO:0000781 chromosome, telomeric region [PMID:11731479]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11731479]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:12626510]
EXISTENCE:direct assay:GO:0016407 acetyltransferase activity [PMID:11731479]
EXISTENCE:direct assay:GO:0031509 subtelomeric heterochromatin formation [PMID:11731479]
EXISTENCE:direct assay:GO:0033255 SAS acetyltransferase complex [PMID:12626510]
EXISTENCE:physical interaction:GO:0033255 SAS acetyltransferase complex [PMID:15788653]
Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
Putative spore wall protein; expression increases during sporulation; not an essential gene; YOR214C has a paralog, SPO19, that arose from the whole genome duplication
hypothetical protein; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:15504911]
EXISTENCE:direct assay:GO:0051020 GTPase binding [PMID:15504911]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10512869|PMID:10562277]
Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:8995429]
EXISTENCE:direct assay:GO:0005663 DNA replication factor C complex [PMID:7651383]
EXISTENCE:genetic interaction:GO:0000278 mitotic cell cycle [PMID:7516465]
EXISTENCE:genetic interaction:GO:0006298 mismatch repair [PMID:9504910]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:6749598|PMID:6749599]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:8770585]
Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
EXISTENCE:direct assay:GO:0004177 aminopeptidase activity [PMID:6339075]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:8308064]
EXISTENCE:direct assay:GO:0007323 peptide pheromone maturation [PMID:6339075]
EXISTENCE:mutant phenotype:GO:0007323 peptide pheromone maturation [PMID:6339075]
Dipeptidyl aminopeptidase; Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
[acyl-carrier-protein] S-malonyltransferase
EXISTENCE:mutant phenotype:GO:0004314 [acyl-carrier-protein] S-malonyltransferase activity [PMID:9388293]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9388293]
EXISTENCE:mutant phenotype:GO:0006631 fatty acid metabolic process [PMID:9388293]
Predicted malonyl-CoA:ACP transferase; putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
mitochondrial 2-oxodicarboxylate carrier
EXISTENCE:direct assay:GO:0005310 dicarboxylic acid transmembrane transporter activity [PMID:11013234]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11013234]
EXISTENCE:direct assay:GO:0006839 mitochondrial transport [PMID:11013234]
Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism; ODC2 has a paralog, ODC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:7630735]
H/ACA box small nucleolar RNA (snoRNA); predicted to guide pseudouridylation of small subunit (SSU) rRNA at position 1191
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:21777356]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:21777356]
Subunit of the DSC ubiquitin ligase complex; hypothetical protein that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
DNA-directed RNA polymerase core subunit RPB8
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9837969]
EXISTENCE:genetic interaction:GO:0006383 transcription by RNA polymerase III [PMID:9927738]
EXISTENCE:physical interaction:GO:0006360 transcription by RNA polymerase I [PMID:11486042]
EXISTENCE:physical interaction:GO:0006366 transcription by RNA polymerase II [PMID:11486042]
EXISTENCE:physical interaction:GO:0006383 transcription by RNA polymerase III [PMID:11486042]
RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10468587|PMID:15143178]
EXISTENCE:genetic interaction:GO:0002098 tRNA wobble uridine modification [PMID:17283054]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10468587]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10588895|PMID:15143178]
Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:genetic interaction:GO:0007029 endoplasmic reticulum organization [PMID:18667535]
hypothetical protein; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
ARS1523; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:23781023|PMID:16407407]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:23781023]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:23781023]
EXISTENCE:genetic interaction:GO:0055088 lipid homeostasis [PMID:23781023]
Mitochondrial hypothetical protein involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains
ETC7; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; located between divergently transcribed genes MCP1 and WTM2
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:16980392]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9234739]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:9234739]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16980392]
EXISTENCE:genetic interaction:GO:0010674 negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:9234739]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16980392]
EXISTENCE:genetic interaction:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:9234739]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:16980392]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:16980392]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:16452505]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16980392]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16452505]
Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9234739|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:16432237]
EXISTENCE:mutant phenotype:GO:0051457 maintenance of protein location in nucleus [PMID:16452505]
EXISTENCE:physical interaction:GO:0006606 protein import into nucleus [PMID:16432237]
Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; acts as a nuclear anchor to retain the ribonucleotide reductase small subunit heterodimer, Rnr2p-Rnr4p, in the nucleus when cells are not in S phase; contains WD repeats
mitogen-activated protein kinase kinase MKK1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12361575]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12361575|PMID:23924898]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12361575|PMID:23924898]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12361575]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:8386320]
EXISTENCE:genetic interaction:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:8386320]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:8386320]
MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:9048947|PMID:12756240]
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:14517234|PMID:14638855]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8022808]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9973563]
EXISTENCE:mutant phenotype:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:8918457]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:8022808]
Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress
putative serine/threonine protein kinase KIN4
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:17967947]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:16039590]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:16039591]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:16039591]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:24850890]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:16039590|PMID:16039591]
EXISTENCE:mutant phenotype:GO:0071988 protein localization to spindle pole body [PMID:24850890]
Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains; null mutant displays increased levels of spontaneous Rad52 foci
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689|PMID:8455623]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:15231838|PMID:12150911]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313|PMID:11081632]
EXISTENCE:genetic interaction:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7556076]
EXISTENCE:genetic interaction:GO:0019843 rRNA binding [PMID:7556076]
EXISTENCE:mutant phenotype:GO:0019843 rRNA binding [PMID:7556076]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
Small nucleolar RNA (snoRNA) U3; part of Small ribosomal SubUnit (SSU) processosome; SNR17B also encodes snoRNA U3
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:1427091|PMID:2827121|PMID:2838385]
EXISTENCE:direct assay:GO:0004146 dihydrofolate reductase activity [PMID:2838385]
EXISTENCE:mutant phenotype:GO:0004146 dihydrofolate reductase activity [PMID:2827121|PMID:1427091]
EXISTENCE:mutant phenotype:GO:0046452 dihydrofolate metabolic process [PMID:1427091]
EXISTENCE:mutant phenotype:GO:0046654 tetrahydrofolate biosynthetic process [PMID:1427091]
Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
oxysterol-binding protein related protein HES1
EXISTENCE:direct assay:GO:0008142 oxysterol binding [PMID:16408938]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:20008566]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:20008566]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:16585271]
Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); SWAT-GFP and mCherry fusion proteins localize to the bud neck and vacuolar membrane; HES1 has a paralog, KES1, that arose from the whole genome duplication
ARS1524; Autonomously Replicating Sequence
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
tRNA(Thr) (cytosine(32)-N(3))-methyltransferase
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:9467951]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:9467951]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:11805329]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:9467951]
EXISTENCE:direct assay:GO:0052735 tRNA (cytosine-3-)-methyltransferase activity [PMID:21518804|PMID:21518805]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:21518804|PMID:21518805]
EXISTENCE:mutant phenotype:GO:0052735 tRNA (cytosine-3-)-methyltransferase activity [PMID:21518804]
AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
EXISTENCE:direct assay:GO:0004326 tetrahydrofolylpolyglutamate synthase activity [PMID:10775416]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10775416]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10775416]
EXISTENCE:mutant phenotype:GO:0006730 one-carbon metabolic process [PMID:10775416]
Folylpolyglutamate synthetase; catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:12073037]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:28223369]
EXISTENCE:direct assay:GO:0031954 positive regulation of protein autophosphorylation [PMID:23207907]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:17110477]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:12073037]
EXISTENCE:mutant phenotype:GO:0031954 positive regulation of protein autophosphorylation [PMID:23207907]
Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; expression controlled by a tightly regulated middle-meiotic promoter that is activated by Ndt80p; translation of SSP2 mRNA is delayed, such that the mRNA is present as nuclear divisions are taking place but is not engaged by ribosomes until relatively late in meiotic development
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25219674|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25219674]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:12682021]
EXISTENCE:direct assay:GO:0031120 snRNA pseudouridine synthesis [PMID:12682021]
EXISTENCE:mutant phenotype:GO:0000455 enzyme-directed rRNA pseudouridine synthesis [PMID:18332121]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:18332121|PMID:12682021]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:14561887]
EXISTENCE:mutant phenotype:GO:0031120 snRNA pseudouridine synthesis [PMID:14561887|PMID:12682021]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25219674|PMID:25192136]
Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria
NuA4 histone acetyltransferase complex catalytic subunit ESA1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10911987]
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:31699900]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:17274630]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0006354 DNA-templated transcription elongation [PMID:15949446]
EXISTENCE:direct assay:GO:0010485 histone H4 acetyltransferase activity [PMID:12110674]
EXISTENCE:direct assay:GO:0032777 Piccolo NuA4 histone acetyltransferase complex [PMID:12782659]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:10911987|PMID:15485911]
EXISTENCE:direct assay:GO:0140068 histone crotonyltransferase activity [PMID:31699900]
EXISTENCE:genetic interaction:GO:0000183 rDNA heterochromatin formation [PMID:16436512]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:25628362]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:19822662]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:16436512]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:10487762]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:12353039]
EXISTENCE:mutant phenotype:GO:0006354 DNA-templated transcription elongation [PMID:15949446]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11036083]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:22539722]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:19822662]
EXISTENCE:mutant phenotype:GO:0034212 peptide N-acetyltransferase activity [PMID:23050233]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:10082517]
Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation
EXISTENCE:direct assay:GO:0004144 diacylglycerol O-acyltransferase activity [PMID:20225889]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:11751830|PMID:24868093]
EXISTENCE:direct assay:GO:0006672 ceramide metabolic process [PMID:22738231]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:21321129]
EXISTENCE:direct assay:GO:0019432 triglyceride biosynthetic process [PMID:11751830]
EXISTENCE:mutant phenotype:GO:0004144 diacylglycerol O-acyltransferase activity [PMID:11751830]
EXISTENCE:mutant phenotype:GO:0006672 ceramide metabolic process [PMID:22738231]
EXISTENCE:mutant phenotype:GO:0019432 triglyceride biosynthetic process [PMID:11751830]
EXISTENCE:mutant phenotype:GO:0019915 lipid storage [PMID:11751830]
EXISTENCE:mutant phenotype:GO:0035356 cellular triglyceride homeostasis [PMID:27620384]
Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25546499]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:14562095|PMID:28540421]
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:28540421]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:21912603]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:21912603]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:28540421]
Conserved oxidoreductase involved in lipid droplet morphology; mutant shows defects in CPY processing and vacuolar morphology; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts; homologus to human RDH12 linked to Leber Congenital Amaurosis
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:13679573]
EXISTENCE:mutant phenotype:GO:0006139 nucleobase-containing compound metabolic process [PMID:9744871]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15972461]
Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:12399376]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
cleavage polyadenylation factor subunit CLP1
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11344258]
EXISTENCE:direct assay:GO:0031124 mRNA 3'-end processing [PMID:11344258]
Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation and in gene looping which affects reinitiation of transcription; mutations in human ortholog cause cerebellar neurodegeneration
EXISTENCE:direct assay:GO:0004792 thiosulfate sulfurtransferase activity [PMID:19151091]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0002143 tRNA wobble position uridine thiolation [PMID:19145231|PMID:19151091]
EXISTENCE:mutant phenotype:GO:0004792 thiosulfate sulfurtransferase activity [PMID:19151091]
Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; involved in metabolism of sterol esters; may be mitochondrially localized
hypothetical protein that associates with ribosomes
peptide alpha-N-acetyltransferase subunit NAT5
EXISTENCE:curator inference:GO:0006474 N-terminal protein amino acid acetylation [PMID:14517307]
EXISTENCE:direct assay:GO:0031415 NatA complex [PMID:14517307]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:25886145]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:25886145]
Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:14562095|PMID:27831485]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:mutant phenotype:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:11226176]
EXISTENCE:mutant phenotype:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:15671059]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:15671059]
EXISTENCE:mutant phenotype:GO:0046967 cytosol to endoplasmic reticulum transport [PMID:15671059]
EXISTENCE:physical interaction:GO:0031207 Sec62/Sec63 complex [PMID:2000150]
Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:15590821]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:19369420]
Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitination and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005825 half bridge of spindle pole body [PMID:8408222]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:9153752]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:18160718]
EXISTENCE:mutant phenotype:GO:0043549 regulation of kinase activity [PMID:9813095]
Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
DNA 5'-adenosine monophosphate hydrolase
EXISTENCE:direct assay:GO:0033699 DNA 5'-adenosine monophosphate hydrolase activity [PMID:16964241]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:20399152]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:20399152]
DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
proteasome regulatory particle base subunit RPT4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10419517]
EXISTENCE:direct assay:GO:0019904 protein domain specific binding [PMID:11418596]
EXISTENCE:genetic interaction:GO:0006289 nucleotide-excision repair [PMID:11410533]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:18174173]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:11389845]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:12447385]
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
translation initiation factor eIF2B subunit gamma
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8506384|PMID:9472020]
EXISTENCE:direct assay:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8506384]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:8506384]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8506384|PMID:9472020]
EXISTENCE:genetic interaction:GO:0006446 regulation of translational initiation [PMID:3516411]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:11707417|PMID:2038327]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:1986242|PMID:2038327|PMID:3516411|PMID:3915540]
EXISTENCE:mutant phenotype:GO:1903574 negative regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:physical interaction:GO:0003743 translation initiation factor activity [PMID:2038327]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:9472020]
EXISTENCE:physical interaction:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:9472020]
Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
proteasome regulatory particle lid subunit RPN8
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27053109]
Essential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:14690591]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:23267056]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:18439903]
Putative GTPase with a specific role in RNA pol II and polIII biogenesis; involved in the assembly and subsequent nuclear import of RNA polymerases II and III; required for establishment of sister chromatid cohesion; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:22842922]
Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress; interacts with Csm1p
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:11739729]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:10978276|PMID:11739729]
EXISTENCE:mutant phenotype:GO:0048487 beta-tubulin binding [PMID:11739729]
Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10490599]
EXISTENCE:genetic interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:10490599]
EXISTENCE:physical interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926]
Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
putative serine/threonine protein kinase HRK1
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:11003661]
Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
hypothetical protein; sporulation is abnormal in homozygous diploid; SWAT-GFP fusion protein localizes to the nucleus; YOR268C is not an essential gene
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15965467]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:15965467|PMID:12566428]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:15965467]
EXISTENCE:direct assay:GO:1903033 positive regulation of microtubule plus-end binding [PMID:25380312]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:12566428]
Regulator of dynein, involved in intracellular transport; part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
H(+)-transporting V0 sector ATPase subunit a
EXISTENCE:direct assay:GO:0000220 vacuolar proton-transporting V-type ATPase, V0 domain [PMID:1385813]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11853670]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:18048916|PMID:22842922|PMID:27881666|PMID:23836928]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:direct assay:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:1385813|PMID:8798414]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:31023825]
EXISTENCE:genetic interaction:GO:0071474 cellular hyperosmotic response [PMID:31023825]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:9933515]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:1385813]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:1385813|PMID:8798414]
EXISTENCE:mutant phenotype:GO:1903778 protein localization to vacuolar membrane [PMID:34342352]
Subunit a of the vacuolar-ATPase V0 domain; encodes one of two isoforms, located in vacuolar V-ATPase complexes while STV1 encodes the second isoform and is located in Golgi and endosomal V-ATPase complexes; interaction with PI(3,5)P2 increases V-ATPase activity and contributes to hyperosmotic stress tolerance; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0071454 cellular response to anoxia [PMID:23467670]
Putative protein; predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9160748]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U960 and U986
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11583614]
EXISTENCE:direct assay:GO:0070545 PeBoW complex [PMID:16287855]
EXISTENCE:direct assay:GO:0110136 protein-RNA complex remodeling [PMID:20542003]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:10454593]
EXISTENCE:physical interaction:GO:0070545 PeBoW complex [PMID:16287855]
Ribosomal assembly factor and 66S pre-ribosomal particle constituent; subunit of the Nop7-subcomplex (PeBoW complex), required for an early nucleolar step in pre-60S ribosomal particle maturation; interaction of its ubiquitin-like (UBL) domain with the MIDAS domain in the Rea1p tail triggers release of the subcomplex and possibly other biogenesis factors via cycles of ATP hydrolysis; involved in the processing of 27S pre-rRNA; contains an N-terminal UBL domain and seven C-terminal WD repeats
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12562762]
EXISTENCE:mutant phenotype:GO:0000296 spermine transport [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000297 spermine transmembrane transporter activity [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0015606 spermidine transmembrane transporter activity [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0015848 spermidine transport [PMID:11171066]
Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:23898186]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9872948]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9872948]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9872948|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9872948]
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:18852462]
EXISTENCE:direct assay:GO:0052381 tRNA dimethylallyltransferase activity [PMID:3031456|PMID:18852462]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:3031456]
Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27803246]
EXISTENCE:direct assay:GO:0016485 protein processing [PMID:11698381]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:11698381|PMID:18202364]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:11698381]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:11698381]
Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation; localizes to cytosol, nucleus and peroxisomes
EXISTENCE:direct assay:GO:0008190 eukaryotic initiation factor 4E binding [PMID:9707439]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:9118949]
EXISTENCE:mutant phenotype:GO:0010606 positive regulation of cytoplasmic mRNA processing body assembly [PMID:18182159]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:9144215]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:17041186]
Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translational initiation; translational activator of select mRNAs during filamentous growth; competes with eIF4G for binding to eIF4E
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:9472021|PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at position U999
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U1004 and U1124
EXISTENCE:mutant phenotype:GO:0004852 uroporphyrinogen-III synthase activity [PMID:7597845]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:18042043|PMID:14559249]
Uroporphyrinogen III synthase; catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
EXISTENCE:genetic interaction:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:18990212]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12612074]
EXISTENCE:physical interaction:GO:0000118 histone deacetylase complex [PMID:12612074]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:12612074]
Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication
Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; localizes to cytosol and peroxisomes; similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:19501098]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:19501098]
EXISTENCE:direct assay:GO:0031683 G-protein beta/gamma-subunit complex binding [PMID:10749875]
EXISTENCE:mutant phenotype:GO:0003779 actin binding [PMID:19501098]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10749875]
EXISTENCE:physical interaction:GO:0005737 cytoplasm [PMID:10749875]
Protein that interacts with the CCT complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP; CCT is short for chaperonin containing TCP-1; essential gene
EXISTENCE:direct assay:GO:0016311 dephosphorylation [PMID:19753119]
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:19753119|PMID:20427268]
Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
Cytoplasmic hypothetical protein; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
EXISTENCE:direct assay:GO:0004792 thiosulfate sulfurtransferase activity [PMID:24981631]
EXISTENCE:direct assay:GO:0006790 sulfur compound metabolic process [PMID:30217845]
Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene
EXISTENCE:direct assay:GO:0004792 thiosulfate sulfurtransferase activity [PMID:19864628]
Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:20038530]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:20038530]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]
EXISTENCE:mutant phenotype:GO:0000469 cleavage involved in rRNA processing [PMID:20038530]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:20038530]
Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
EXISTENCE:direct assay:GO:0019153 protein-disulfide reductase (glutathione) activity [PMID:16002399]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16002399]
Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
hypothetical protein; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:23437238]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1901413|PMID:22932476|PMID:22842922]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:8016655]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]
EXISTENCE:direct assay:GO:0031492 nucleosomal DNA binding [PMID:18644858]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:20126658]
EXISTENCE:direct assay:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:8127913|PMID:8458575]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:1459453]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1901413]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:10198436]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16024655]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:1459453|PMID:8016655]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:31846549]
EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF complex [PMID:8159677]
EXISTENCE:mutant phenotype:GO:0031496 positive regulation of mating type switching [PMID:6436497]
EXISTENCE:mutant phenotype:GO:0035973 aggrephagy [PMID:31846549]
EXISTENCE:mutant phenotype:GO:0042148 strand invasion [PMID:19766565]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1339306|PMID:3542227|PMID:8016655|PMID:10549298|PMID :17681272]
EXISTENCE:mutant phenotype:GO:0070577 lysine-acetylated histone binding [PMID:12419247]
EXISTENCE:mutant phenotype:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:8016655|PMID:8458575]
EXISTENCE:mutant phenotype:GO:1900189 positive regulation of cell adhesion involved in single-species biofilm formation [PMID:18202364]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11865042]
Catalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19182805|PMID:19001347]
Vacuolar protein; involved in oxidative stress response and modulation of replicative lifespan; targeted to vacuole via AP-3 pathway; undergoes autophosphorylation, which is abolished by mutation of Asp781 in catalytic motif (D781N); P5 ATPase subfamily member with similarity to yeast Spf1p and homology to human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication; mutations in the human homolog associated with Diamond-Blackfan anemia
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:10688653]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10688653]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17938242|PMID:17443350]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:21822217]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:15135061]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10688653]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:10688653]
Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
EXISTENCE:direct assay:GO:0000500 RNA polymerase I upstream activating factor complex [PMID:11500378]
EXISTENCE:direct assay:GO:0001165 RNA polymerase I cis-regulatory region sequence-specific DNA binding [PMID:18451108]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748]
EXISTENCE:mutant phenotype:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:18765638]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:20154141]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:11500378|PMID:18765638]
Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene
ARS1526; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:12637749]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:10648604|PMID:10637294]
EXISTENCE:genetic interaction:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10637294]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17922641]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:17922641]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17922641]
EXISTENCE:mutant phenotype:GO:0046685 response to arsenic-containing substance [PMID:17922641]
Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
hypothetical protein involved in outer spore wall organization; has similarity to the tafazzins superfamily of acyltransferases
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9710580]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:9710580]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9710580]
Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0030140 trans-Golgi network transport vesicle [PMID:16498409]
EXISTENCE:direct assay:GO:0034044 exomer complex [PMID:17000877]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:8657162]
EXISTENCE:physical interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:16498409]
Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:15356260|PMID:25416945]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14980713|PMID:15356260]
EXISTENCE:genetic interaction:GO:0007121 bipolar cellular bud site selection [PMID:14980713]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:14980713]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:25416945]
Protein involved in establishing bud site selection; localizes to the bud neck and previous sites of cell division (bud scars or cytokinesis remnants (CRMs); localization to both sites is interdependent with Rax2p; required with Rax2p to anchor Nba1p and Nis1p to CRMs where a Cdc42p inhibitory zone is established, preventing repolarization of cells at previous division sites; predicted type IIIa transmembrane protein with similarity to members of the insulin-related peptide superfamily
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:10608810]
EXISTENCE:direct assay:GO:0006417 regulation of translation [PMID:10608810]
EXISTENCE:direct assay:GO:0045182 translation regulator activity [PMID:10608810]
CPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA
carbamoyl-phosphate synthase (glutamine-hydrolyzing) CPA1
EXISTENCE:direct assay:GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [PMID:8626695]
EXISTENCE:direct assay:GO:0005951 carbamoyl-phosphate synthase complex [PMID:206535]
EXISTENCE:mutant phenotype:GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [PMID:6086650]
EXISTENCE:mutant phenotype:GO:0006526 arginine biosynthetic process [PMID:9290206]
Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16227570]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:19203228]
EXISTENCE:direct assay:GO:0005880 nuclear microtubule [PMID:10620773]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:16227570]
EXISTENCE:direct assay:GO:0008623 CHRAC [PMID:14673157]
EXISTENCE:direct assay:GO:0015616 DNA translocase activity [PMID:19203228]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:23468649]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:12504018]
EXISTENCE:genetic interaction:GO:0060195 negative regulation of antisense RNA transcription [PMID:18075583]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12624196]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:17689493]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:11238381|PMID:16227570]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:14673157]
ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; exhibits basal levels of chromatin binding throughout the genome as well as interacts with sequence-specific factors to accurately position nucleosomes in chromatin; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:23671312]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:23671312|PMID:14562095]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:23671312]
Protein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16204239]
EXISTENCE:genetic interaction:GO:0032217 riboflavin transmembrane transporter activity [PMID:16204239]
EXISTENCE:genetic interaction:GO:0032218 riboflavin transport [PMID:16204239]
Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23613772|PMID:14562095]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:1903839]
Protein involved in ER-to-Golgi transport; packaged into COPII vesicles for trafficking between ER and Golgi
EXISTENCE:genetic interaction:GO:0000481 maturation of 5S rRNA [PMID:19806183]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10449419|PMID:11720284]
EXISTENCE:mutant phenotype:GO:0000481 maturation of 5S rRNA [PMID:19806183]
Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9372940]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10094313]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10094313]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10094313]
Protein involved in producing mature rRNAs and snoRNAs; involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
EXISTENCE:direct assay:GO:0004143 diacylglycerol kinase activity [PMID:18458075|PMID:18458076]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11481671]
EXISTENCE:direct assay:GO:0006654 phosphatidic acid biosynthetic process [PMID:18458076]
EXISTENCE:mutant phenotype:GO:0006654 phosphatidic acid biosynthetic process [PMID:18458075]
EXISTENCE:mutant phenotype:GO:2001210 regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway [PMID:32058036]
Diacylglycerol kinase; localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures, stimulates the mevalonate/ergosterol pathway and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:23874617]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:23874617]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:23874617]
Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
EXISTENCE:expression pattern:GO:0030437 ascospore formation [PMID:3540611]
Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16289536|PMID:14562095]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:16289536]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:19033152]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:16289536]
EXISTENCE:mutant phenotype:GO:0036267 invasive filamentous growth [PMID:32665277]
EXISTENCE:mutant phenotype:GO:0090033 positive regulation of filamentous growth [PMID:32665277]
EXISTENCE:mutant phenotype:GO:0098609 cell-cell adhesion [PMID:32665277]
Putative transcription factor; induces superficial pseudohyphal growth, positively regulates invasive growth, but is not required for invasive pseudohyphal growth; may act together with Phd1p; promotes cell adhesion independent of Flo11p by repressing genes that encode cell wall degrading enzymes; localizes to the nucleus; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:9712830]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762|PMID:27881666|PMID:23836928]
EXISTENCE:genetic interaction:GO:0005385 zinc ion transmembrane transporter activity [PMID:8058041]
EXISTENCE:genetic interaction:GO:0006829 zinc ion transport [PMID:8058041]
EXISTENCE:mutant phenotype:GO:0006824 cobalt ion transport [PMID:1508175]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:10856230]
EXISTENCE:mutant phenotype:GO:0015087 cobalt ion transmembrane transporter activity [PMID:1508175]
Vacuolar transporter that mediates zinc transport into vacuole; overexpression confers resistance to cobalt and rhodium; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
ARS1528; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27136724|PMID:16622836]
EXISTENCE:direct assay:GO:0031956 medium-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:genetic interaction:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:11477098|PMID:22633490]
EXISTENCE:genetic interaction:GO:0035336 long-chain fatty-acyl-CoA metabolic process [PMID:11477098]
EXISTENCE:genetic interaction:GO:0044539 long-chain fatty acid import into cell [PMID:12601005|PMID:27136724]
EXISTENCE:genetic interaction:GO:1905329 sphingoid long-chain base transport [PMID:27136724]
EXISTENCE:mutant phenotype:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:11477098]
EXISTENCE:mutant phenotype:GO:0035336 long-chain fatty-acyl-CoA metabolic process [PMID:11477098]
EXISTENCE:mutant phenotype:GO:0044539 long-chain fatty acid import into cell [PMID:12601005|PMID:27136724]
EXISTENCE:mutant phenotype:GO:1905329 sphingoid long-chain base transport [PMID:27136724]
Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9436903]
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:15565172|PMID:16314500]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9436903]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:16314500]
U2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:12651885]
EXISTENCE:direct assay:GO:0008375 acetylglucosaminyltransferase activity [PMID:12651885]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:12651885]
N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus
dolichyl-phosphate-mannose-protein mannosyltransferase PMT3
EXISTENCE:direct assay:GO:0097583 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex [PMID:12551906]
EXISTENCE:direct assay:GO:0097585 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex [PMID:12551906]
EXISTENCE:genetic interaction:GO:0035269 protein O-linked mannosylation [PMID:9184828]
Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:18976803]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:18976803]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:22118465]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762|PMID:22118465]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22118465|PMID:18976803]
EXISTENCE:mutant phenotype:GO:0002092 positive regulation of receptor internalization [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:18976803|PMID:32744498]
EXISTENCE:physical interaction:GO:0070086 ubiquitin-dependent endocytosis [PMID:18976803]
Alpha-arrestin, Ub-ligase adaptor for Rsp5p; regulates starvation- and substrate-induced Ub-dependent endocytosis of select plasma membrane localized amino acid transporters, recruiting Rsp5p to its targets; role in basal internalization and turnover of Ste2p, mating and zygote formation; recruits Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, affecting cytosol and plasma membrane translocation; localization regulated by Rsp5p-dependent ubiquitination
EXISTENCE:direct assay:GO:0004350 glutamate-5-semialdehyde dehydrogenase activity [PMID:12513997]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31323700]
EXISTENCE:direct assay:GO:0006561 proline biosynthetic process [PMID:12513997]
Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15189990|PMID:12514182|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0071469 cellular response to alkaline pH [PMID:15189990]
EXISTENCE:mutant phenotype:GO:0071472 cellular response to salt stress [PMID:15189990]
EXISTENCE:physical interaction:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:21097665]
Tail-anchored ER membrane hypothetical protein; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8188749]
EXISTENCE:direct assay:GO:0000146 microfilament motor activity [PMID:11381095]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:8978821]
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:8294515]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:8188749]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:8188749]
EXISTENCE:direct assay:GO:0030133 transport vesicle [PMID:23079598]
EXISTENCE:direct assay:GO:0031941 filamentous actin [PMID:11381095]
EXISTENCE:direct assay:GO:0031982 vesicle [PMID:12456647]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:8188749|PMID:19053807]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:11381095]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:12391144]
EXISTENCE:genetic interaction:GO:0000011 vacuole inheritance [PMID:12594460]
EXISTENCE:genetic interaction:GO:0006904 vesicle docking involved in exocytosis [PMID:27307583]
EXISTENCE:genetic interaction:GO:0007107 membrane addition at site of cytokinesis [PMID:12456647]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:15201867|PMID:12391144]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:12391144]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:8978821]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:10984058]
EXISTENCE:mutant phenotype:GO:0007107 membrane addition at site of cytokinesis [PMID:12456647]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:2016335]
EXISTENCE:mutant phenotype:GO:0009826 unidimensional cell growth [PMID:9864365]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:2016335]
EXISTENCE:mutant phenotype:GO:0030050 vesicle transport along actin filament [PMID:10562281|PMID:9864365]
EXISTENCE:mutant phenotype:GO:0032432 actin filament bundle [PMID:9864365]
EXISTENCE:mutant phenotype:GO:0034993 meiotic nuclear membrane microtubule tethering complex [PMID:32059771]
EXISTENCE:mutant phenotype:GO:0045033 peroxisome inheritance [PMID:11733545]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:12391144|PMID:15201867]
EXISTENCE:mutant phenotype:GO:0048313 Golgi inheritance [PMID:11285273]
EXISTENCE:physical interaction:GO:0000011 vacuole inheritance [PMID:12594460]
EXISTENCE:physical interaction:GO:0000132 establishment of mitotic spindle orientation [PMID:10984058]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27307583]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:12391144]
EXISTENCE:physical interaction:GO:0045033 peroxisome inheritance [PMID:16678774]
EXISTENCE:physical interaction:GO:0071563 Myo2p-Vac17p-Vac8p transport complex [PMID:12594460]
Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, lipid droplets, and the mitotic spindle; effector of Ypt11p, a rab-type small GTPase; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:16452633]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16452633|PMID:36125415]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:36125415]
EXISTENCE:direct assay:GO:0030658 transport vesicle membrane [PMID:15821745]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:11029060]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:9195971]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:8374953]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:9731774]
Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; Snc2p levels regulated by Vps45p; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication
ATP-binding cassette multidrug transporter PDR10
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19452121]
ATP-binding cassette (ABC) transporter; multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p
EXISTENCE:direct assay:GO:0008157 protein phosphatase 1 binding [PMID:12356757]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:12181333]
EXISTENCE:genetic interaction:GO:0030866 cortical actin cytoskeleton organization [PMID:12181333]
EXISTENCE:genetic interaction:GO:2000370 positive regulation of clathrin-dependent endocytosis [PMID:12356757]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:16251346]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:8688556]
EXISTENCE:mutant phenotype:GO:0030866 cortical actin cytoskeleton organization [PMID:12956961]
EXISTENCE:mutant phenotype:GO:2000370 positive regulation of clathrin-dependent endocytosis [PMID:12356757]
EXISTENCE:physical interaction:GO:0008157 protein phosphatase 1 binding [PMID:8754819]
Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:20601675]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10567545|PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0006264 mitochondrial DNA replication [PMID:12023279]
EXISTENCE:direct assay:GO:0008408 3'-5' exonuclease activity [PMID:20601675]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:29519802]
EXISTENCE:mutant phenotype:GO:0006264 mitochondrial DNA replication [PMID:2684980]
EXISTENCE:mutant phenotype:GO:0032043 mitochondrial DNA catabolic process [PMID:29519802]
Mitochondrial DNA polymerase gamma; single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoan complex of catalytic and accessory subunits; polymorphic in yeast, petites occur more frequently in some lab strains; human ortholog POLG complements yeast mip1 mutant; mutations in human POLG associated with Alpers-Huttenlocher syndrome (AHS), progressive external ophthalmoplegia (PEO), parkinsonism, other mitochondrial diseases
H(+)-transporting V1 sector ATPase subunit E
EXISTENCE:direct assay:GO:0000221 vacuolar proton-transporting V-type ATPase, V1 domain [PMID:16774922]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:23836928|PMID:26928762]
Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10400670]
EXISTENCE:direct assay:GO:0015095 magnesium ion transmembrane transporter activity [PMID:12628916]
EXISTENCE:direct assay:GO:0045016 mitochondrial magnesium ion transmembrane transport [PMID:12628916]
EXISTENCE:mutant phenotype:GO:0015095 magnesium ion transmembrane transporter activity [PMID:10400670]
EXISTENCE:mutant phenotype:GO:0045016 mitochondrial magnesium ion transmembrane transport [PMID:10400670]
Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095|PMID:16556230]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16556230|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0004813 alanine-tRNA ligase activity [PMID:7761427]
EXISTENCE:mutant phenotype:GO:0006419 alanyl-tRNA aminoacylation [PMID:7761427]
Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis and with hereditary peripheral neuropathy, Charcot-Marie-Tooth (CMT) disease
EXISTENCE:direct assay:GO:0003980 UDP-glucose:glycoprotein glucosyltransferase activity [PMID:9611196]
EXISTENCE:mutant phenotype:GO:0070880 fungal-type cell wall beta-glucan biosynthetic process [PMID:9430631]
Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:8524314]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:8524314]
EXISTENCE:direct assay:GO:0051285 cell cortex of cell tip [PMID:23673619]
Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
hypothetical protein; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication
putative E2 ubiquitin-protein ligase UBC11
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:8982460]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29519818]
Ubiquitin-conjugating enzyme; most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
DNA-directed RNA polymerase I subunit RPA43
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7592632]
DNA-directed RNA polymerase I core subunit RPA190
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:3054507|PMID:9837969]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15520468]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:27184763]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:2023944]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:3054507]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
YORWTy2-2; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:10606743]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:10606743]
EXISTENCE:genetic interaction:GO:0045821 positive regulation of glycolytic process [PMID:10606743]
EXISTENCE:mutant phenotype:GO:0045821 positive regulation of glycolytic process [PMID:10606743]
Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix
ARS1529; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16546083]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:8751446]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16452144]
EXISTENCE:direct assay:GO:0017125 deoxycytidyl transferase activity [PMID:8751446]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:8751446]
EXISTENCE:direct assay:GO:0070987 error-free translesion synthesis [PMID:8751446]
EXISTENCE:genetic interaction:GO:0042276 error-prone translesion synthesis [PMID:9765213]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:17248528]
EXISTENCE:mutant phenotype:GO:0070987 error-free translesion synthesis [PMID:10540291]
EXISTENCE:physical interaction:GO:0005657 replication fork [PMID:17517887]
Bifunctional DNA-directed DNA polymerase/deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress; may be involved in meiosis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558]
EXISTENCE:mutant phenotype:GO:0004743 pyruvate kinase activity [PMID:9139918]
EXISTENCE:mutant phenotype:GO:0006090 pyruvate metabolic process [PMID:9139918]
Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:28679684]
EXISTENCE:direct assay:GO:0015175 neutral L-amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0015804 neutral amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0015812 gamma-aminobutyric acid transport [PMID:8455553]
EXISTENCE:direct assay:GO:0015824 proline transport [PMID:10654085]
EXISTENCE:genetic interaction:GO:0015193 L-proline transmembrane transporter activity [PMID:14968425]
EXISTENCE:genetic interaction:GO:0015812 gamma-aminobutyric acid transport [PMID:8455553]
EXISTENCE:mutant phenotype:GO:0015193 L-proline transmembrane transporter activity [PMID:3552672]
Proline permease; required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:2407611]
EXISTENCE:genetic interaction:GO:0007021 tubulin complex assembly [PMID:9215891]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9215891]
Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:21257754]
EXISTENCE:direct assay:GO:1990904 ribonucleoprotein complex [PMID:21257754]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:21257754]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:21257754]
Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:27483004|PMID:9832507]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7926789|PMID:24390141]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:10786836]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:1752435]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:10848609]
Meiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:22411836|PMID:27381459]
Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12972564]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12972564]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:12972564]
Key component of the RAM signaling network; required for proper cell morphogenesis and cell separation after mitosis
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:26780366]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511544]
EXISTENCE:mutant phenotype:GO:0070124 mitochondrial translational initiation [PMID:30767393]
Multicopy suppressor of HER2 involved in mitochondrial translation; mutant is defective in directing meiotic recombination events to homologous chromatids
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:34694912]
EXISTENCE:direct assay:GO:0062060 NuA4 histone acetyltransferase complex binding [PMID:34694912]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8750239]
EXISTENCE:mutant phenotype:GO:0043967 histone H4 acetylation [PMID:34694912]
Protein involved in histone H4 acetylation at ribosomal protein genes; interacts with and modulates NuA4 histone acetyltransferase complex; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a segment predicted to resemble the winged-helix/forkhead domain located in DNA-binding proteins
putative electron-transferring-flavoprotein dehydrogenase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239|PMID:14576278]
Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17420293]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9456318]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:9456318]
EXISTENCE:mutant phenotype:GO:0034499 late endosome to Golgi transport [PMID:12554655]
Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7828851]
EXISTENCE:direct assay:GO:0016602 CCAAT-binding factor complex [PMID:7828851]
EXISTENCE:genetic interaction:GO:0043457 regulation of cellular respiration [PMID:7828851]
EXISTENCE:mutant phenotype:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:12614847]
EXISTENCE:physical interaction:GO:0016602 CCAAT-binding factor complex [PMID:7828851]
Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18469165]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:16429156|PMID:12858164]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20007605]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11445562]
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:20007605]
EXISTENCE:direct assay:GO:0070336 flap-structured DNA binding [PMID:20007605]
EXISTENCE:mutant phenotype:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:18469165]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:12858164|PMID:16429156]
EXISTENCE:physical interaction:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:18469165]
EXISTENCE:physical interaction:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:20007605]
Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity; forms [SMAUG+] prion
3',5'-cyclic-nucleotide phosphodiesterase PDE2
EXISTENCE:direct assay:GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity [PMID:3025832]
EXISTENCE:mutant phenotype:GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway [PMID:8391474]
High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
translation initiation factor eIF3 core subunit b
EXISTENCE:direct assay:GO:0002183 cytoplasmic translational initiation [PMID:7798228]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:9671501|PMID:7876188]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9671501|PMID:9660829]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:9671501]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:19470581|PMID:24291094|PMID:26777405]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:11018020|PMID:15838098]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:3029094]
EXISTENCE:physical interaction:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9660829]
eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough
proteasome core particle subunit alpha 7
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:9087403]
EXISTENCE:curator inference:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:9087403]
Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
oleate-activated transcription factor PIP2
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9288897]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10428786]
EXISTENCE:direct assay:GO:0089716 Pip2-Oaf1 complex [PMID:9288897]
EXISTENCE:genetic interaction:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:9288897]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9288897]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8972187|PMID:10428786]
EXISTENCE:mutant phenotype:GO:0032000 positive regulation of fatty acid beta-oxidation [PMID:8670793|PMID:8972187]
EXISTENCE:mutant phenotype:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:8670793|PMID:8972187|PMID:9288897]
EXISTENCE:physical interaction:GO:0089716 Pip2-Oaf1 complex [PMID:8972187]
Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
putative flavin adenine dinucleotide transporter
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:27462707]
Putative protein involved in sphingolipid biosynthesis; not an essential gene; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:12857851]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:12857851]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:12857851]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:12857851]
Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:12604797]
EXISTENCE:direct assay:GO:0030896 checkpoint clamp complex [PMID:12604797|PMID:9891048]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:9564050]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:17624540]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511543]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:11356855]
Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins
EXISTENCE:direct assay:GO:1990145 maintenance of translational fidelity [PMID:32408794]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:32408794]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:32408794]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog
EXISTENCE:direct assay:GO:0004663 Rab geranylgeranyltransferase activity [PMID:8183917]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9202009|PMID:22842922]
EXISTENCE:direct assay:GO:0005968 Rab-protein geranylgeranyltransferase complex [PMID:8183917|PMID:8583924]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9202009]
EXISTENCE:mutant phenotype:GO:0004663 Rab geranylgeranyltransferase activity [PMID:8183917]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:8183917]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8132658]
EXISTENCE:mutant phenotype:GO:0018344 protein geranylgeranylation [PMID:12972569]
EXISTENCE:mutant phenotype:GO:0031267 small GTPase binding [PMID:12972569]
EXISTENCE:mutant phenotype:GO:0090630 activation of GTPase activity [PMID:7750142|PMID:8132658]
Rab escort protein; type II geranylgeranyltransferase complex (Bet2p-Bet4p) chaperone that complexes with newly synthesized Rab GTPases, like Ypt1p and Sec4p, leading to their prenylation and membrane delivery; modulates the TOR pathway through interactions with Sfp1p; alters the kinetics of MAPK pathway activation and polarity reorganization during filamentous growth; sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:20160012]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16030250]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:20160012]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:genetic interaction:GO:0004862 cAMP-dependent protein kinase inhibitor activity [PMID:16278446|PMID:16924114]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:12150916]
EXISTENCE:genetic interaction:GO:0042326 negative regulation of phosphorylation [PMID:16278446]
EXISTENCE:genetic interaction:GO:0046580 negative regulation of Ras protein signal transduction [PMID:16793550]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:20160012]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0010255 glucose mediated signaling pathway [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0046579 positive regulation of Ras protein signal transduction [PMID:20160012]
EXISTENCE:physical interaction:GO:0032794 GTPase activating protein binding [PMID:16793550]
Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:10207056]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10207056]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:10207056]
EXISTENCE:mutant phenotype:GO:0090282 positive regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:10207056]
Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes; turnover is tightly regulated during cell cycle and in response to DNA damage
EXISTENCE:direct assay:GO:0061499 outer plaque of mitotic spindle pole body [PMID:9585415]
EXISTENCE:genetic interaction:GO:0031536 positive regulation of exit from mitosis [PMID:11101520]
EXISTENCE:genetic interaction:GO:0051293 establishment of spindle localization [PMID:22385961]
EXISTENCE:mutant phenotype:GO:0000073 initial mitotic spindle pole body separation [PMID:22385961]
EXISTENCE:mutant phenotype:GO:0030953 astral microtubule organization [PMID:11101520]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:11101520|PMID:23579499]
EXISTENCE:mutant phenotype:GO:0035591 signaling adaptor activity [PMID:23579499]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:16888627]
EXISTENCE:mutant phenotype:GO:0051293 establishment of spindle localization [PMID:22385961]
EXISTENCE:physical interaction:GO:0035591 signaling adaptor activity [PMID:23579499]
Mitotic exit network (MEN) scaffold protein; core component of the SPB outer plaque that functions in astral microtubule organization via Spc72p; scaffold for recruitment of MEN components, such as Cdc15p, Tem1p and the Bfa1p-Bub2p checkpoint complex; phosphorylation by Cdc15p creates a Dbf2p-Mob1p phospho-docking site enabling Cdc15p-dependent complex activation and mitotic exit; acts through the MEN to specify Kar9p-mediated asymmetric SPB inheritance; homologous to mammalian centriolin
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:9473035]
EXISTENCE:direct assay:GO:0004030 aldehyde dehydrogenase [NAD(P)+] activity [PMID:9473035]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431]
EXISTENCE:genetic interaction:GO:0006740 NADPH regeneration [PMID:19158096]
EXISTENCE:genetic interaction:GO:0019413 acetate biosynthetic process [PMID:10919763]
EXISTENCE:mutant phenotype:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:9675847]
EXISTENCE:mutant phenotype:GO:0006067 ethanol metabolic process [PMID:9675847]
EXISTENCE:mutant phenotype:GO:0006090 pyruvate metabolic process [PMID:10409655]
Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant
EXISTENCE:direct assay:GO:0004354 glutamate dehydrogenase (NADP+) activity [PMID:11562373|PMID:2932370|PMID:2989290]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:4126]
EXISTENCE:mutant phenotype:GO:0004354 glutamate dehydrogenase (NADP+) activity [PMID:2932370|PMID:2989290]
EXISTENCE:mutant phenotype:GO:0006537 glutamate biosynthetic process [PMID:2932370|PMID:2989290]
NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YOR376W is not an essential gene
EXISTENCE:direct assay:GO:0004026 alcohol O-acetyltransferase activity [PMID:8085822]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:15042596]
EXISTENCE:genetic interaction:GO:1901089 acetate ester metabolic process involved in fermentation [PMID:12957907]
EXISTENCE:mutant phenotype:GO:1901089 acetate ester metabolic process involved in fermentation [PMID:12957907|PMID:23200656]
Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:24707045]
EXISTENCE:direct assay:GO:0008519 ammonium transmembrane transporter activity [PMID:24707045]
EXISTENCE:genetic interaction:GO:0072488 ammonium transmembrane transport [PMID:24707045]
Low affinity NH4+ transporter; member of the DHA2 family of drug:H+ anti porters; putative paralog of ATR1; but not required for boron tolerance; non-essential gene
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:11882665]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:11882665]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:11882665]
Transcriptional repressor involved in regulating multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11120744]
EXISTENCE:genetic interaction:GO:0000293 ferric-chelate reductase activity [PMID:11120744]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10341420|PMID:9726978]
EXISTENCE:mutant phenotype:GO:0015891 siderophore transport [PMID:9726978]
Ferric reductase; reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
EXISTENCE:direct assay:GO:0015891 siderophore transport [PMID:12196168]
Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall
Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
EXISTENCE:direct assay:GO:0003904 deoxyribodipyrimidine photo-lyase activity [PMID:2911265]
DNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p
hypothetical protein; regulated by the metal-responsive Aft1p transcription factor; highly inducible in zinc-depleted conditions; localizes to the soluble fraction; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YOR387C has a paralog, VEL1, that arose from a single-locus duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11921099]
EXISTENCE:direct assay:GO:0008863 formate dehydrogenase (NAD+) activity [PMID:21246355]
EXISTENCE:genetic interaction:GO:0042183 formate catabolic process [PMID:19564482]
NAD(+)-dependent formate dehydrogenase; may protect cells from exogenous formate
hypothetical protein; expression regulated by copper levels
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:26055717]
EXISTENCE:genetic interaction:GO:1903424 fluoride transmembrane transport [PMID:24173035|PMID:26055717]
EXISTENCE:genetic interaction:GO:1903425 fluoride transmembrane transporter activity [PMID:24173035]
EXISTENCE:mutant phenotype:GO:1903424 fluoride transmembrane transport [PMID:26055717]
Protein involved in fluoride export; nearly identical to FEX2, and deletion of both proteins results in a large increase in fluoride sensitivity compared with the single mutant; contains two FEX domains connected by a linker; part of a widespread family of conserved fluoride export proteins
glutathione-independent methylglyoxalase family protein
EXISTENCE:mutant phenotype:GO:0031669 cellular response to nutrient levels [PMID:24706893]
Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to E. coli Hsp31 and S. cerevisiae Hsp31p, Hsp32p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the cytosol; identical to Pau22p; encodes two proteins that are translated from 2 different start codons
TEL15R; Telomeric region on the right arm of Chromosome XV; composed of an X element core sequence, X element combinatorial repeats, a long Y' element, and a very short terminal stretch of telomeric repeats
Y' element ATP-dependent helicase protein 1 copy 8
One of several telomeric Y' element-encoded DNA helicases; known as Y'-Help1 (Y'-HELicase Protein 1)