Saccharomyces cerevisiae S288C chromosome XIV, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (26-MAY-2010) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006947.\n\nOn Apr 26, 2011 this sequence version replaced NC_001146.7.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL14L; Telomeric region on the left arm of Chromosome XIV; composed of an X element core sequence, X element combinatorial repeats, a short stretch of telomeric repeats, and a long Y' element
Y' element ATP-dependent helicase protein 1 copy 6
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9837911]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
EXISTENCE:direct assay:GO:0018820 cyanamide hydratase activity [PMID:25847245]
EXISTENCE:mutant phenotype:GO:0018890 cyanamide metabolic process [PMID:25847245]
Cyanamide hydratase that detoxifies cyanamide; member of the HD domain metalloprotein superfamily; expression is induced over 100-fold by cyanamide and by SN2-type DNA alkylating agents such as MMS and DMA; induction decreased in rad6 and rad18 mutants; gene and protein are identical to DDI3 and Ddi3p
putative pyridoxal 5'-phosphate synthase
hypothetical protein; nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
EXISTENCE:genetic interaction:GO:0008615 pyridoxine biosynthetic process [PMID:30498136]
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:30498136]
EXISTENCE:genetic interaction:GO:0042819 vitamin B6 biosynthetic process [PMID:12271461]
Protein involved in thiamine and pyridoxine biosynthesis; member of a stationary phase-induced gene family where transcriptional induction precedes the diauxic shift; induced in the absence of thiamin in a Thi2/3p-dependent manner and repressed in its presence; forms a co-regulated gene pair with SNO2; interacts with Thi11p; paralog of SNZ1 and SNZ3
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:12777485]
Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
EXISTENCE:direct assay:GO:0047681 aryl-alcohol dehydrogenase (NADP+) activity [PMID:29079624]
Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
EXISTENCE:direct assay:GO:0000118 histone deacetylase complex [PMID:8962081]
EXISTENCE:direct assay:GO:0004407 histone deacetylase activity [PMID:12110674]
EXISTENCE:direct assay:GO:0032221 Rpd3S/Clr6-CII complex [PMID:16286007|PMID:16286008]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:direct assay:GO:0070211 Snt2C complex [PMID:19040720]
EXISTENCE:direct assay:GO:0070822 Sin3-type complex [PMID:9234741]
EXISTENCE:genetic interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:genetic interaction:GO:0000117 regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:17908798]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11069890]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:19948887]
EXISTENCE:genetic interaction:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:15143171]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17210643|PMID:17296735]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15141165|PMID:16314178|PMID:17121596|PMID:20398213|P MID:24358376|PMID:24881874]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:14737171]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:9150136]
EXISTENCE:mutant phenotype:GO:0004407 histone deacetylase activity [PMID:12110674|PMID:8962081|PMID:9512514|PMID:9572144]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:22539722]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:14609951|PMID:19270272]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:12453428|PMID:15143171|PMID:19417103]
EXISTENCE:mutant phenotype:GO:0034503 protein localization to nucleolar rDNA repeats [PMID:17203076]
EXISTENCE:mutant phenotype:GO:0044804 autophagy of nucleus [PMID:31553911]
EXISTENCE:mutant phenotype:GO:0045128 negative regulation of reciprocal meiotic recombination [PMID:18515193]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15254041|PMID:17210643|PMID:17296735|PMID:17706600|P MID:20398213]
EXISTENCE:mutant phenotype:GO:0051038 negative regulation of transcription involved in meiotic cell cycle [PMID:17158929]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:10388812|PMID:10512855|PMID:19372273]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:10082585|PMID:10388812|PMID:10512855]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:31553911]
EXISTENCE:physical interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:physical interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11069890]
EXISTENCE:physical interaction:GO:0003713 transcription coactivator activity [PMID:14737171]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:9150136]
Histone deacetylase, component of both Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the Hmt1p methyltransferase; delays late firing of single copy origins by opposing the Fkh1/2 origin activation pathway
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:12808025]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12808025]
EXISTENCE:direct assay:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:direct assay:GO:0043335 protein unfolding [PMID:29321502]
EXISTENCE:genetic interaction:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:mutant phenotype:GO:0005777 peroxisome [PMID:12808025]
EXISTENCE:mutant phenotype:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:15634331]
AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:16027163]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9325105]
EXISTENCE:genetic interaction:GO:0030150 protein import into mitochondrial matrix [PMID:16027163]
EXISTENCE:physical interaction:GO:0001405 PAM complex, Tim23 associated import motor [PMID:16027163]
EXISTENCE:physical interaction:GO:0030150 protein import into mitochondrial matrix [PMID:16027163]
Constituent of the mitochondrial import motor; associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:27229769]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:13679573]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
EXISTENCE:direct assay:GO:0030428 cell septum [PMID:15452134]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:8668141]
Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase; localizes in birth scars; required for proper cell separation after cytokinesis; expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16301533|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16186255]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:16186255]
EXISTENCE:direct assay:GO:0018345 protein palmitoylation [PMID:16186255]
EXISTENCE:mutant phenotype:GO:0000324 fungal-type vacuole [PMID:16301533]
EXISTENCE:mutant phenotype:GO:0016409 palmitoyltransferase activity [PMID:26224664]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:16186255|PMID:16301533]
Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
ARS1405; Autonomously Replicating Sequence
phosphatidylinositol-3,5-bisphosphate 5-phosphatase
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14528018]
EXISTENCE:direct assay:GO:0043813 phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity [PMID:14528018]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:14528018]
EXISTENCE:direct assay:GO:0070772 PAS complex [PMID:19037259]
EXISTENCE:genetic interaction:GO:0046856 phosphatidylinositol dephosphorylation [PMID:11950935]
EXISTENCE:physical interaction:GO:0070772 PAS complex [PMID:19037259]
Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12133835|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12133835]
EXISTENCE:genetic interaction:GO:0044088 regulation of vacuole organization [PMID:25359886]
EXISTENCE:mutant phenotype:GO:0007166 cell surface receptor signaling pathway [PMID:11063677]
EXISTENCE:mutant phenotype:GO:0015247 aminophospholipid flippase activity [PMID:12133835]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:12842877]
EXISTENCE:mutant phenotype:GO:0140345 phosphatidylcholine flippase activity [PMID:12133835]
EXISTENCE:physical interaction:GO:1990531 phospholipid-translocating ATPase complex [PMID:15090616]
Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11956747]
Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:17588950]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20709757|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:20578725]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026036]
EXISTENCE:mutant phenotype:GO:0006813 potassium ion transport [PMID:17588950]
EXISTENCE:mutant phenotype:GO:0006814 sodium ion transport [PMID:17588950]
EXISTENCE:mutant phenotype:GO:0015369 calcium:proton antiporter activity [PMID:20578725]
EXISTENCE:mutant phenotype:GO:0015385 sodium:proton antiporter activity [PMID:17588950]
EXISTENCE:mutant phenotype:GO:0015386 potassium:proton antiporter activity [PMID:17588950]
EXISTENCE:mutant phenotype:GO:1902600 proton transmembrane transport [PMID:20578725]
Calcium/H+ antiporter localized to endoplasmic reticulum membrane; member of calcium exchanger (CAX) family; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005354 galactose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
Protein with similarity to hexose transporter family members; expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
cleavage polyadenylation factor subunit PFS2
EXISTENCE:curator inference:GO:0003723 RNA binding [PMID:12819204]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:10619842]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:10619842]
Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0004664 prephenate dehydratase activity [PMID:1532796]
EXISTENCE:mutant phenotype:GO:0009094 L-phenylalanine biosynthetic process [PMID:1532796]
Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1532796|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10681564|PMID:12829692]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:2142305]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:2142305]
EXISTENCE:physical interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:10681564]
Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo
EXISTENCE:curator inference:GO:0003700 DNA-binding transcription factor activity []
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10702289]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:8367295]
EXISTENCE:mutant phenotype:GO:0000256 allantoin catabolic process [PMID:1898922]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:10906145]
EXISTENCE:mutant phenotype:GO:0043565 sequence-specific DNA binding [PMID:10702289]
EXISTENCE:physical interaction:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:10906145]
Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
tetratricopeptide repeat-containing protein EMW1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21273246|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21273246|PMID:14562095|PMID:14690591]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:21273246]
Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9679065]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:7761422]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:8804316]
EXISTENCE:direct assay:GO:0005662 DNA replication factor A complex [PMID:2554144]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:2554144]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:8910442]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:12226081]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:7761422]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:9679065]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:14702040]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:9093844]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:9093844]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:8910442]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:14702040]
EXISTENCE:mutant phenotype:GO:0010833 telomere maintenance via telomere lengthening [PMID:22354040]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:14702040]
EXISTENCE:physical interaction:GO:0000722 telomere maintenance via recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0006312 mitotic recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0007131 reciprocal meiotic recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0016567 protein ubiquitination [PMID:18342608]
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s
putative SCF ubiquitin ligase complex subunit SKP2
EXISTENCE:curator inference:GO:0004842 ubiquitin-protein transferase activity [PMID:14747994]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:20936605]
EXISTENCE:mutant phenotype:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:20936605]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
F-box hypothetical protein; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:15992824|PMID:15383543]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:15719019]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15719019]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:15992824]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:15719019]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:15383543]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:16460754]
Protein co-chaperone with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix; required for the maintenance of Ssc1p solubility and assists in the functional interaction of Ssc1p with substrate proteins
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0030907 MBF transcription complex [PMID:18794370]
EXISTENCE:direct assay:GO:0033309 SBF transcription complex [PMID:18794370]
EXISTENCE:direct assay:GO:0070822 Sin3-type complex [PMID:9393435]
EXISTENCE:genetic interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10409718]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9393435]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:18794370]
EXISTENCE:physical interaction:GO:0003713 transcription coactivator activity [PMID:10409718|PMID:12832490|PMID:18794370]
EXISTENCE:physical interaction:GO:0070822 Sin3-type complex [PMID:9393435]
Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11027267]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:22180534]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11027267]
Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
serine/threonine/tyrosine protein kinase MCK1
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:23314252]
EXISTENCE:direct assay:GO:0004712 protein serine/threonine/tyrosine kinase activity [PMID:8407952]
EXISTENCE:genetic interaction:GO:0030163 protein catabolic process [PMID:17954914|PMID:23236290]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:36321416]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:2010084]
EXISTENCE:mutant phenotype:GO:0004857 enzyme inhibitor activity [PMID:22918234]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:12399380]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:17954914|PMID:23236290]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:2010083]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:2010083]
EXISTENCE:physical interaction:GO:0030332 cyclin binding [PMID:22918234]
Dual-specificity S/T and tyrosine protein kinase; spindle position checkpoint component that works in parallel with Kin4p to prevent mitotic exit network (MEN) activation by the Cdc14 early anaphase release (FEAR) network; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent degradation of Rcn1p and Cdc6p, inhibition of protein kinase A, transcriptional regulation, inhibition of RNAPIII, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division
mitochondrial 37S ribosomal protein YmS18
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:21673659|PMID:21673967|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:21673659]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:21673967]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:21673659|PMID:21673967]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:21673967]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:21673967]
Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12391144]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:23427260]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16980630|PMID:23427260]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12391144|PMID:23427260]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12391144|PMID:23427260]
EXISTENCE:direct assay:GO:0048309 endoplasmic reticulum inheritance [PMID:23427260]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:18245340|PMID:23427260]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:12391144|PMID:15201867]
EXISTENCE:genetic interaction:GO:0048313 Golgi inheritance [PMID:18595704]
EXISTENCE:genetic interaction:GO:0090167 Golgi distribution to daughter cells [PMID:18595704]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:18245340]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:16980630|PMID:23427260]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:12391144]
EXISTENCE:mutant phenotype:GO:0048313 Golgi inheritance [PMID:18595704]
EXISTENCE:mutant phenotype:GO:0051646 mitochondrion localization [PMID:23139336]
EXISTENCE:mutant phenotype:GO:0090167 Golgi distribution to daughter cells [PMID:18595704]
EXISTENCE:physical interaction:GO:0031489 myosin V binding [PMID:12391144]
Rab GTPase; Myo2p-binding protein implicated in mother-to-bud transport of cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during cell division; function is regulated at multiple levels; abundance of active Ypt11p forms is controlled by phosphorylation status and degradation; normally a low-abundance protein whose ER localization is only detected when protein is highly over expressed
ARS1406; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16159874|PMID:16246728]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:16159874]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:16159874]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:16159874]
Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
EXISTENCE:direct assay:GO:0004652 polynucleotide adenylyltransferase activity [PMID:16260630|PMID:16374505]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:16374505]
EXISTENCE:genetic interaction:GO:0004652 polynucleotide adenylyltransferase activity [PMID:16374505]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:16373491]
EXISTENCE:genetic interaction:GO:0043629 ncRNA polyadenylation [PMID:16373491|PMID:16374505|PMID:16431988]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:17652137]
EXISTENCE:genetic interaction:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:15935758|PMID:16373491]
EXISTENCE:genetic interaction:GO:0071037 nuclear polyadenylation-dependent snRNA catabolic process [PMID:15935758]
EXISTENCE:genetic interaction:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:16431988]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18007593]
EXISTENCE:genetic interaction:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:16373491]
EXISTENCE:genetic interaction:GO:0071044 histone mRNA catabolic process [PMID:17179095]
EXISTENCE:genetic interaction:GO:0071050 sno(s)RNA polyadenylation [PMID:18951092]
EXISTENCE:genetic interaction:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:16373491]
EXISTENCE:mutant phenotype:GO:0004652 polynucleotide adenylyltransferase activity [PMID:16374505]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:16373491]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:18007593]
Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:19218422]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:22588722]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:19218422]
EXISTENCE:direct assay:GO:0016301 kinase activity [PMID:14993234]
EXISTENCE:direct assay:GO:0019899 enzyme binding [PMID:31395335]
EXISTENCE:genetic interaction:GO:0000011 vacuole inheritance [PMID:19218422]
EXISTENCE:genetic interaction:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:genetic interaction:GO:0019236 response to pheromone [PMID:17189484]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:7649470]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:19218422]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0031106 septin ring organization [PMID:11025657]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0042307 positive regulation of protein import into nucleus [PMID:22588722]
Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12052896]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16301316]
EXISTENCE:direct assay:GO:0006623 protein targeting to vacuole [PMID:16219684]
EXISTENCE:direct assay:GO:0031901 early endosome membrane [PMID:16219684]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:12052896]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:12052896]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:16301316]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:12052896]
Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins
EXISTENCE:direct assay:GO:0000262 mitochondrial chromosome [PMID:31532710]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:31532710]
EXISTENCE:mutant phenotype:GO:0090144 mitochondrial nucleoid organization [PMID:31532710]
EXISTENCE:mutant phenotype:GO:1901858 regulation of mitochondrial DNA metabolic process [PMID:31532710]
Mitochondrial ribosome-associated protein; regulates mitochondrial DNA copy number and the size and distribution of mtDNA nucleoids; subunit of a complex containing Pet20p, Mam33p, and Pim1p that may regulate mtDNA replication; member of the PET20 domain-containing protein family; forms foci that partially co-localize with mtDNA
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23019326]
EXISTENCE:genetic interaction:GO:0009272 fungal-type cell wall biogenesis [PMID:16524906]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:10821185]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:16524906]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:10821185]
EXISTENCE:mutant phenotype:GO:0070613 regulation of protein processing [PMID:27803246]
EXISTENCE:mutant phenotype:GO:0071469 cellular response to alkaline pH [PMID:23019326]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:27803246]
pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10679030]
EXISTENCE:direct assay:GO:0000133 polarisome [PMID:16166638]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22593206]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10559187|PMID:22593206]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:22593206]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22593206]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22593206]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10679030|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:22593206|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:35134079]
EXISTENCE:direct assay:GO:0090630 activation of GTPase activity [PMID:22593206]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:12913108]
EXISTENCE:genetic interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:16166638]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:22593206]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:22593206]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:22593206]
EXISTENCE:mutant phenotype:GO:0043087 regulation of GTPase activity [PMID:22593206]
EXISTENCE:physical interaction:GO:0006897 endocytosis [PMID:22593206]
EXISTENCE:physical interaction:GO:0032880 regulation of protein localization [PMID:22593206]
EXISTENCE:physical interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:16166638]
Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; localizes to plasma membrane, sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:9358157]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:9358157]
EXISTENCE:genetic interaction:GO:0009982 pseudouridine synthase activity [PMID:9358157]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:9358157]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9358157]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25192136|PMID:25219674]
Pseudouridine synthase; catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; also responsible for pseudouracil modification of some mRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14729456]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10958666]
EXISTENCE:direct assay:GO:0006816 calcium ion transport [PMID:10958666]
EXISTENCE:direct assay:GO:0015275 stretch-activated, cation-selective, calcium channel activity [PMID:10436155]
EXISTENCE:mutant phenotype:GO:0005262 calcium channel activity [PMID:12492866|PMID:9343395]
Stretch-activated Ca2+-permeable cation channel; required for Ca2+ influx stimulated by mating pheromones and some abiotic stresses; sensor of compressive mechanical stress; N-glycosylated membrane protein of the ER and plasma membrane; interacts with Cch1p; oligomerizes; is an alpha2/delta-like protein and participates in the generation of stretch-activated Ca2+-permeable cation channel activity when expressed in mammalian cells
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:12604797|PMID:8995429]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276|PMID:22932476]
EXISTENCE:direct assay:GO:0005663 DNA replication factor C complex [PMID:7651383]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006272 leading strand elongation [PMID:1346062]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:8302859]
EXISTENCE:direct assay:GO:0031389 Rad17 RFC-like complex [PMID:12604797|PMID:10660302]
EXISTENCE:physical interaction:GO:0031389 Rad17 RFC-like complex [PMID:10660302]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
EXISTENCE:physical interaction:GO:0031391 Elg1 RFC-like complex [PMID:12912927]
Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7973730]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:14688790]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14688790]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:7973730]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:19458192]
EXISTENCE:genetic interaction:GO:0032878 regulation of establishment or maintenance of cell polarity [PMID:17853895]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:20417603]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:7973730]
EXISTENCE:physical interaction:GO:0032878 regulation of establishment or maintenance of cell polarity [PMID:17853895]
Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114|PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:8674114|PMID:10024243]
EXISTENCE:direct assay:GO:0000452 snoRNA guided rRNA 2'-O-methylation [PMID:25653162]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U898 and small subunit (SSU) rRNA at position G1271
ARS1407; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:11733989]
Component of the CCR4-NOT transcriptional complex; evolutionarily conserved; involved in controlling mRNA initiation, elongation, and degradation; binds Cdc39p
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17101773]
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:1461285]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:1461285]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8001155]
Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:26631165|PMID:17403781]
EXISTENCE:direct assay:GO:0034337 RNA folding [PMID:26631165]
EXISTENCE:genetic interaction:GO:1903241 U2-type prespliceosome assembly [PMID:9710584]
EXISTENCE:mutant phenotype:GO:0030620 U2 snRNA binding [PMID:26631165|PMID:9710584]
EXISTENCE:mutant phenotype:GO:0034337 RNA folding [PMID:26631165]
EXISTENCE:mutant phenotype:GO:1903241 U2-type prespliceosome assembly [PMID:17403781]
EXISTENCE:physical interaction:GO:0005686 U2 snRNP [PMID:26631165]
EXISTENCE:physical interaction:GO:1903241 U2-type prespliceosome assembly [PMID:9710584]
Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA stem II region between different structures; contains two RNA recognition motifs (RRMs)
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons
ARS1409; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
YNLCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
mitochondrial 54S ribosomal protein YmL10/YmL18
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:9151978]
Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20652590|PMID:27337501]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:11545731]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:mutant phenotype:GO:0004888 transmembrane signaling receptor activity [PMID:10430578]
EXISTENCE:mutant phenotype:GO:0007266 Rho protein signal transduction [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10348843]
Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9620854]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9029160]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9029160]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9029160]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9029160]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380|PMID:9029160]
EXISTENCE:physical interaction:GO:0005655 nucleolar ribonuclease P complex [PMID:9029160]
Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:12504007]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:12504007]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:12504007]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:12504007]
Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0050613 delta14-sterol reductase activity [PMID:32908]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:1394506|PMID:1418625|PMID:8125337]
EXISTENCE:mutant phenotype:GO:0050613 delta14-sterol reductase activity [PMID:1394506|PMID:8125337]
C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:11062271]
EXISTENCE:mutant phenotype:GO:0032220 plasma membrane fusion involved in cytogamy [PMID:11062271|PMID:15590839]
Pheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:25028499]
Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; contains a PH-like domain; CAF120 has a paralog, SKG3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004414 homoserine O-acetyltransferase activity [PMID:3301801]
EXISTENCE:direct assay:GO:0009092 homoserine metabolic process [PMID:3301801]
EXISTENCE:mutant phenotype:GO:0000096 sulfur amino acid metabolic process [PMID:1101032]
EXISTENCE:mutant phenotype:GO:0004414 homoserine O-acetyltransferase activity [PMID:3301801]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:6035500]
EXISTENCE:mutant phenotype:GO:0009092 homoserine metabolic process [PMID:3301801]
L-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16565073]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11401825|PMID:12447444]
EXISTENCE:direct assay:GO:0046713 borate transport [PMID:17459946]
EXISTENCE:direct assay:GO:0080139 borate efflux transmembrane transporter activity [PMID:17459946]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:16565073]
EXISTENCE:mutant phenotype:GO:0046713 borate transport [PMID:12447444|PMID:16923078|PMID:17459946]
EXISTENCE:mutant phenotype:GO:0080139 borate efflux transmembrane transporter activity [PMID:12447444|PMID:16923078|PMID:17459946]
Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
EXISTENCE:mutant phenotype:GO:0009436 glyoxylate catabolic process [PMID:17173333]
EXISTENCE:mutant phenotype:GO:0047964 glyoxylate reductase (NAD+) activity [PMID:17173333]
Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:17483413]
EXISTENCE:mutant phenotype:GO:0000076 DNA replication checkpoint signaling [PMID:12944972]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:16137625]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:17483413]
EXISTENCE:mutant phenotype:GO:0043111 replication fork arrest [PMID:16103218|PMID:16418273|PMID:17652453]
EXISTENCE:mutant phenotype:GO:0043570 maintenance of DNA repeat elements [PMID:18321795]
EXISTENCE:mutant phenotype:GO:0048478 replication fork protection [PMID:16418273|PMID:18632578]
Subunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia
ARS1410; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:9199166]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12045183]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:20493815]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:20493815|PMID:26928762]
EXISTENCE:direct assay:GO:0030133 transport vesicle [PMID:12045183]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:20493815]
EXISTENCE:genetic interaction:GO:0006914 autophagy [PMID:20444978]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:12045183]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:20444978]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:29293994]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:29293994]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:20493815]
Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11154270]
EXISTENCE:direct assay:GO:0000133 polarisome [PMID:16166638|PMID:9571251]
EXISTENCE:direct assay:GO:0005522 profilin binding [PMID:15923184|PMID:12134165]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:12052901]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11154270]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11154270]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:26771880]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9082982|PMID:19053807]
EXISTENCE:direct assay:GO:0045010 actin nucleation [PMID:12052901]
EXISTENCE:direct assay:GO:0051016 barbed-end actin filament capping [PMID:14561409]
EXISTENCE:direct assay:GO:0070649 formin-nucleated actin cable assembly [PMID:12134165]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:genetic interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:11740491]
EXISTENCE:genetic interaction:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:12419188]
EXISTENCE:genetic interaction:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:10085293]
EXISTENCE:mutant phenotype:GO:0005522 profilin binding [PMID:15923184]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:10085294|PMID:16166638]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:mutant phenotype:GO:0070649 formin-nucleated actin cable assembly [PMID:11740491]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521]
EXISTENCE:physical interaction:GO:0000133 polarisome [PMID:9571251]
Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1
EXISTENCE:direct assay:GO:0015174 basic amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0015802 basic amino acid transport [PMID:10654085]
Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
hypothetical protein; protein-coding gene that evolved de novo via a series of point mutations in noncoding sequence; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; may be involved in DNA repair pathway during stationary phase and contribute to robustness of cells when shifted to a nutrient-poor environment
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:29402931]
Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
EXISTENCE:direct assay:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:8194527|PMID:8248783]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10587649|PMID:16365163|PMID:8194527]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:10587649|PMID:16365163]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:8194527|PMID:8248783]
EXISTENCE:mutant phenotype:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:10587649]
EXISTENCE:mutant phenotype:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:10587649]
EXISTENCE:mutant phenotype:GO:0140504 microlipophagy [PMID:32726584]
EXISTENCE:mutant phenotype:GO:2000786 positive regulation of autophagosome assembly [PMID:31125705]
Phosphatidylinositol 4-kinase; catalyzes the first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; required for autophagosome formation during autophagy and for lipophagy in both stationary phase cells and during nitrogen starvation; may control nonselective autophagy and mitophagy through trafficking of Atg9p; localizes to the nucleus and to the trans-Golgi network
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18032584]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18032584|PMID:36125415|PMID:14562095]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:18032582]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:18032584]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:18032582]
EXISTENCE:mutant phenotype:GO:0042030 ATPase inhibitor activity [PMID:36125415]
EXISTENCE:mutant phenotype:GO:0099638 endosome to plasma membrane protein transport [PMID:36125415]
Protein involved in recycling of membrane transporters from endosome to plasma membrane; plays a role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20016005]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12869188|PMID:26928762]
EXISTENCE:direct assay:GO:0008526 phosphatidylinositol transfer activity [PMID:10799527|PMID:10848624]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:10799527|PMID:10848624]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:12869188]
EXISTENCE:genetic interaction:GO:0008526 phosphatidylinositol transfer activity [PMID:9890948]
EXISTENCE:genetic interaction:GO:0008654 phospholipid biosynthetic process [PMID:9890948]
EXISTENCE:genetic interaction:GO:0009410 response to xenobiotic stimulus [PMID:9890948]
EXISTENCE:genetic interaction:GO:0015914 phospholipid transport [PMID:9890948]
EXISTENCE:genetic interaction:GO:0016126 sterol biosynthetic process [PMID:9890948]
EXISTENCE:genetic interaction:GO:0043001 Golgi to plasma membrane protein transport [PMID:10848624]
EXISTENCE:genetic interaction:GO:1901352 negative regulation of phosphatidylglycerol biosynthetic process [PMID:12869188]
EXISTENCE:genetic interaction:GO:2001247 positive regulation of phosphatidylcholine biosynthetic process [PMID:12869188]
EXISTENCE:mutant phenotype:GO:0006658 phosphatidylserine metabolic process [PMID:10799527]
EXISTENCE:mutant phenotype:GO:0008526 phosphatidylinositol transfer activity [PMID:9890948]
EXISTENCE:mutant phenotype:GO:0015914 phospholipid transport [PMID:9890948]
Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10970842]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10970842]
EXISTENCE:physical interaction:GO:0005789 endoplasmic reticulum membrane [PMID:15659647]
Integral membrane protein; required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
DNA polymerase epsilon catalytic subunit
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:16916794|PMID:14622139]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:14622139]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:16369485]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:15773893|PMID:18851837]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:14622139|PMID:16369485]
EXISTENCE:direct assay:GO:0008622 epsilon DNA polymerase complex [PMID:12571237|PMID:2406268]
EXISTENCE:direct assay:GO:0032183 SUMO binding [PMID:31765407]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:19776424]
EXISTENCE:genetic interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18437220]
EXISTENCE:genetic interaction:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:18851837|PMID:8895664]
EXISTENCE:mutant phenotype:GO:0003887 DNA-directed DNA polymerase activity [PMID:1537345]
EXISTENCE:mutant phenotype:GO:0006272 leading strand elongation [PMID:17615360]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:8423775]
EXISTENCE:mutant phenotype:GO:0006297 nucleotide-excision repair, DNA gap filling [PMID:11452038]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:10025407]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18437220]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:12665575]
EXISTENCE:mutant phenotype:GO:0008310 single-stranded DNA 3'-5' exodeoxyribonuclease activity [PMID:1658784]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:8895664]
EXISTENCE:mutant phenotype:GO:0033314 mitotic DNA replication checkpoint signaling [PMID:9792727]
EXISTENCE:mutant phenotype:GO:0035822 gene conversion [PMID:20595613]
EXISTENCE:mutant phenotype:GO:0045004 DNA replication proofreading [PMID:1658784|PMID:21124948]
EXISTENCE:physical interaction:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:18851837]
Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:7579692]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:9038340]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16716188|PMID:7579692]
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23318452|PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:26182403]
EXISTENCE:direct assay:GO:0062092 Yae1-Lto1 complex [PMID:26182403]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:26182403]
EXISTENCE:mutant phenotype:GO:0062092 Yae1-Lto1 complex [PMID:26182403]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:26182403]
Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human ORAOV1, which is overexpressed in solid tumors; co-expression of ORAOV1 and YAE1 homolog YAE1D1 restores growth and Rli1p maturation after LTO1 depletion
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9083054]
EXISTENCE:direct assay:GO:0016531 copper chaperone activity [PMID:17961510|PMID:9346482]
EXISTENCE:mutant phenotype:GO:0006825 copper ion transport [PMID:9083054]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9083054]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:7731983]
Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15942868]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:19346454]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11739780]
EXISTENCE:direct assay:GO:0070939 Dsl1/NZR complex [PMID:19151722]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11493604]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11739780|PMID:11493604]
EXISTENCE:mutant phenotype:GO:0032581 ER-dependent peroxisome organization [PMID:19346454]
EXISTENCE:physical interaction:GO:0070939 Dsl1/NZR complex [PMID:15958492]
Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:8127709]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:26001273]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8127709]
EXISTENCE:mutant phenotype:GO:0032366 intracellular sterol transport [PMID:26001273]
Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p
trifunctional dihydropteroate synthetase/dihydrohydroxymethylpterin pyrophosphokinase/dihydroneopterin aldolase FOL1
EXISTENCE:direct assay:GO:0003848 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity [PMID:15169867]
EXISTENCE:direct assay:GO:0004150 dihydroneopterin aldolase activity [PMID:15169867]
EXISTENCE:direct assay:GO:0004156 dihydropteroate synthase activity [PMID:15169867]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8813764]
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:15169867]
EXISTENCE:mutant phenotype:GO:0004156 dihydropteroate synthase activity [PMID:11731153]
EXISTENCE:mutant phenotype:GO:0046654 tetrahydrofolate biosynthetic process [PMID:11731153|PMID:15169867]
Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
mRNA-binding translational activator GIS2
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640|PMID:23285195]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:21232131]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764|PMID:21232131|PMID:23222640]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23285195]
EXISTENCE:direct assay:GO:0042788 polysomal ribosome [PMID:21277287]
EXISTENCE:direct assay:GO:0045182 translation regulator activity [PMID:21277287]
EXISTENCE:direct assay:GO:2000767 positive regulation of cytoplasmic translation [PMID:21277287]
Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
hypothetical protein; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
EXISTENCE:curator inference:GO:0006406 mRNA export from nucleus [PMID:11979277]
EXISTENCE:direct assay:GO:0000346 transcription export complex [PMID:11979277]
Protein involved in mRNA export; component of the transcription export (TREX) complex
mitochondrial 54S ribosomal protein YmL17/YmL30
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:21084293]
EXISTENCE:direct assay:GO:0001068 transcription regulatory region RNA binding [PMID:21084293]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9618475]
EXISTENCE:direct assay:GO:0019904 protein domain specific binding [PMID:18660819]
EXISTENCE:direct assay:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:16427013]
EXISTENCE:direct assay:GO:0035649 Nrd1 complex [PMID:10655211]
EXISTENCE:direct assay:GO:0071041 antisense RNA transcript catabolic process [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0034472 snRNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0042780 tRNA 3'-end processing [PMID:21460797]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:21113025]
EXISTENCE:mutant phenotype:GO:0071034 CUT catabolic process [PMID:16973436]
EXISTENCE:mutant phenotype:GO:0071041 antisense RNA transcript catabolic process [PMID:22028667]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27076633]
RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:8367302]
EXISTENCE:direct assay:GO:0003691 double-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0004017 adenylate kinase activity [PMID:17349953]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9845372|PMID:22842922]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:15546877]
EXISTENCE:direct assay:GO:0032078 negative regulation of endodeoxyribonuclease activity [PMID:17698079]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:15721260]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:17698079]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:11238918]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:17349953]
EXISTENCE:mutant phenotype:GO:0004017 adenylate kinase activity [PMID:17349953]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:20040573]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:12399380|PMID:9501103|PMID:31390569]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:8207053]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:27173583]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:17349953]
EXISTENCE:mutant phenotype:GO:0097552 mitochondrial double-strand break repair via homologous recombination [PMID:22214610]
EXISTENCE:physical interaction:GO:0030870 Mre11 complex [PMID:9845372]
Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
DNA-directed RNA polymerase I subunit RPA49
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:21263028]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9837969]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:direct assay:GO:0006362 transcription elongation by RNA polymerase I [PMID:18160037]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:9121426]
EXISTENCE:mutant phenotype:GO:0005736 RNA polymerase I complex [PMID:1409638]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:12374750]
RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p
EXISTENCE:direct assay:GO:0004817 cysteine-tRNA ligase activity [PMID:9523015]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31350340]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:31350340]
EXISTENCE:direct assay:GO:0006423 cysteinyl-tRNA aminoacylation [PMID:9523015]
Cysteinyl-tRNA synthetase; CRS1 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form that is mitochondrial; may interact with ribosomes, based on co-purification experiments; human gene CARS allows growth of the yeast haploid null mutant after sporulation of a heterozygous diploid
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:17344218]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:17344218]
EXISTENCE:direct assay:GO:0010698 acetyltransferase activator activity [PMID:19172749]
EXISTENCE:direct assay:GO:0035066 positive regulation of histone acetylation [PMID:18458063]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:17344218]
EXISTENCE:genetic interaction:GO:0035066 positive regulation of histone acetylation [PMID:18458063]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18036332]
EXISTENCE:mutant phenotype:GO:0010698 acetyltransferase activator activity [PMID:19172749]
EXISTENCE:mutant phenotype:GO:0035066 positive regulation of histone acetylation [PMID:18458063]
EXISTENCE:physical interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18036332]
EXISTENCE:physical interaction:GO:0042393 histone binding [PMID:17344218]
NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
U2-type spliceosomal complex subunit CWC25
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:19935684]
EXISTENCE:direct assay:GO:0005684 U2-type spliceosomal complex [PMID:11884590]
Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
EXISTENCE:direct assay:GO:0001731 formation of translation preinitiation complex [PMID:12008673|PMID:15664195|PMID:17434125|PMID:17504939]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:12008673]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:12861028|PMID:15145951]
EXISTENCE:direct assay:GO:0043024 ribosomal small subunit binding [PMID:17434125|PMID:19751744|PMID:12860115]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:15838098|PMID:11018020]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:17504939|PMID:19751744]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:19751744]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:9488467]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:12861028|PMID:15145951|PMID:22851688]
Translation initiation factor eIF1; component of complex involved in recognition of initiator codon; modulates translation accuracy at initiation phase by discriminating against suboptimal initiation sites to prevent excessive uORF translation genome-wide
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11302750]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10397764]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:17151356]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:8335689]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8380177]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:10397764]
Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:11689437|PMID:18497569]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11382760|PMID:22842922]
EXISTENCE:direct assay:GO:0030242 autophagy of peroxisome [PMID:11382760]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:27498190]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:11382761]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:23382696]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:11382760]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:11382761]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
Putative lipid transport protein; peripheral membrane protein required for vesicle formation during autophagy, pexophagy and the CVT pathway; directed to autophagic membranes by Atg18p where it acts as a PAS-ER membrane tether during autophagosome formation; involved in Atg9p cycling between the PAS and mitochondria; binds the phospholipid PI(3)P via an APT1 domain; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0004345 glucose-6-phosphate dehydrogenase activity [PMID:2001672|PMID:2269430]
EXISTENCE:mutant phenotype:GO:0006740 NADPH regeneration [PMID:2269430]
EXISTENCE:mutant phenotype:GO:0009051 pentose-phosphate shunt, oxidative branch [PMID:7045591|PMID:8299150]
EXISTENCE:mutant phenotype:GO:0042542 response to hydrogen peroxide [PMID:7586028|PMID:9480895]
Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidative stress; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant
iron-sulfur cluster assembly protein NAR1
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15103330]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15103330]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:15103330]
Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery; required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; deficiency results in shortened lifespan and sensitivity to paraquat; homologous to human Narf
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:8063738]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:9584198]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:9584198]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0008234 cysteine-type peptidase activity [PMID:8063738]
EXISTENCE:direct assay:GO:0043418 homocysteine catabolic process [PMID:16769724]
EXISTENCE:direct assay:GO:0046677 response to antibiotic [PMID:8063738]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9374524]
EXISTENCE:mutant phenotype:GO:0043418 homocysteine catabolic process [PMID:16769724]
Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; cytosolic protein, selectively transported to vacuole during nitrogen starvation; targeted to vacuole via CVT pathway; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
EXISTENCE:direct assay:GO:0004252 serine-type endopeptidase activity [PMID:2646633]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:8308064]
EXISTENCE:mutant phenotype:GO:0007323 peptide pheromone maturation [PMID:6430565]
Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
Probable type-III integral membrane hypothetical protein; has regions of similarity to mitochondrial electron transport proteins
BEL2; GAL22; MED16; RYE1; SDI3; SSF5; SSN4; SSX3; TSF3
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:9891034]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:20308326]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:genetic interaction:GO:0070202 regulation of establishment of protein localization to chromosome [PMID:23447536]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12558798]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12482986]
EXISTENCE:mutant phenotype:GO:0070202 regulation of establishment of protein localization to chromosome [PMID:23447536]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both positive and negative transcriptional regulation; dispensible for basal transcription
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0020037 heme binding [PMID:9988749]
hypothetical protein with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15470103|PMID:15901837]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15282802]
EXISTENCE:direct assay:GO:0005940 septin ring [PMID:9314530|PMID:15901837]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:15901837]
EXISTENCE:mutant phenotype:GO:0000917 division septum assembly [PMID:15470103]
EXISTENCE:mutant phenotype:GO:0006031 chitin biosynthetic process [PMID:15470103]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12529424|PMID:9314530]
Targeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; phosphorylation by Slt2p and Kss1p involved in regulating Bni4p in septum assembly
EXISTENCE:curator inference:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10465791]
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:10465791]
EXISTENCE:curator inference:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11027292]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11027292]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:15935758|PMID:17643380]
EXISTENCE:genetic interaction:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:11027292|PMID:19060898]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:11027292|PMID:19060898]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:11910110]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; human homolog EXOSC1 partially complements yeast csl4 null mutant, and can complement inviability of strain in which expression of CSL4 is repressed
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:12869188|PMID:24403601]
EXISTENCE:direct assay:GO:0008526 phosphatidylinositol transfer activity [PMID:10848624]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:10848624]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:31690622]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:29187527]
EXISTENCE:mutant phenotype:GO:0043942 negative regulation of sexual sporulation resulting in formation of a cellular spore [PMID:24403601]
Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:17296727]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:17296727]
EXISTENCE:mutant phenotype:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:17296727]
EXISTENCE:physical interaction:GO:0070449 elongin complex [PMID:10430890|PMID:10753924]
Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:15371425]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10748041]
EXISTENCE:direct assay:GO:0051219 phosphoprotein binding [PMID:10604478]
EXISTENCE:genetic interaction:GO:0006808 regulation of nitrogen utilization [PMID:8755910]
EXISTENCE:mutant phenotype:GO:0006808 regulation of nitrogen utilization [PMID:8002570]
EXISTENCE:mutant phenotype:GO:0032447 protein urmylation [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0042994 cytoplasmic sequestering of transcription factor [PMID:10748041|PMID:10799523]
EXISTENCE:physical interaction:GO:0006808 regulation of nitrogen utilization [PMID:8755910]
Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:17242366]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20368619|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17242366|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0005730 nucleolus [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11378903]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:17242366]
Co-chaperone that stimulates the ATPase activity of Ssa1p; required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:24390141]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:11514632]
EXISTENCE:direct assay:GO:0061499 outer plaque of mitotic spindle pole body [PMID:9585415]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:11514632]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:10749924|PMID:9571234]
EXISTENCE:mutant phenotype:GO:0051300 spindle pole body organization [PMID:9571234]
Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:19927118]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:19927118]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:16227579|PMID:19927118]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:19927118]
EXISTENCE:direct assay:GO:0051096 positive regulation of helicase activity [PMID:19927118]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:16945917]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:19927118]
EXISTENCE:genetic interaction:GO:0030490 maturation of SSU-rRNA [PMID:19801658|PMID:19927118]
EXISTENCE:mutant phenotype:GO:0032781 positive regulation of ATP-dependent activity [PMID:19927118]
EXISTENCE:mutant phenotype:GO:0051096 positive regulation of helicase activity [PMID:19927118]
Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22240591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22240591]
EXISTENCE:direct assay:GO:0019786 Atg8-specific peptidase activity [PMID:11038174]
EXISTENCE:direct assay:GO:0051697 protein delipidation [PMID:17632063]
EXISTENCE:mutant phenotype:GO:0006501 C-terminal protein lipidation [PMID:11038174]
EXISTENCE:mutant phenotype:GO:0019786 Atg8-specific peptidase activity [PMID:11038174]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:11149920]
Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation
RNA polymerase II subunit A C-terminal domain phosphatase
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:22235117]
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:12660165]
EXISTENCE:direct assay:GO:0008420 RNA polymerase II CTD heptapeptide repeat phosphatase activity [PMID:15125841|PMID:22235117]
EXISTENCE:direct assay:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:15125841|PMID:22235117]
EXISTENCE:genetic interaction:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:8657130]
EXISTENCE:genetic interaction:GO:0004721 phosphoprotein phosphatase activity [PMID:12660165]
EXISTENCE:genetic interaction:GO:0005634 nucleus [PMID:11046131]
EXISTENCE:genetic interaction:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:11046131]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17101794]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:8657130]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:22235117]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17101794]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:12660165|PMID:22235117]
EXISTENCE:mutant phenotype:GO:0008420 RNA polymerase II CTD heptapeptide repeat phosphatase activity [PMID:15125841|PMID:22235117]
EXISTENCE:mutant phenotype:GO:0009302 sno(s)RNA transcription [PMID:12660165]
EXISTENCE:mutant phenotype:GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled [PMID:12944462|PMID:12819204]
EXISTENCE:mutant phenotype:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:12944462|PMID:12819204]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:12704082]
EXISTENCE:mutant phenotype:GO:0031564 transcription antitermination [PMID:12453421]
EXISTENCE:mutant phenotype:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:15125841|PMID:22235117]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:12704082]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:11046131]
EXISTENCE:physical interaction:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12453421]
EXISTENCE:physical interaction:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:11046131]
Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
EXISTENCE:direct assay:GO:0000243 commitment complex [PMID:2529976]
EXISTENCE:direct assay:GO:0000395 mRNA 5'-splice site recognition [PMID:18084028]
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:2440584]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:2440584]
EXISTENCE:direct assay:GO:0030627 pre-mRNA 5'-splice site binding [PMID:12214237|PMID:18084028]
EXISTENCE:mutant phenotype:GO:0030627 pre-mRNA 5'-splice site binding [PMID:15989965|PMID:3056718]
U1 spliceosomal RNA (U1 snRNA); snRNA U1 homolog
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:23700311|PMID:26135751]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:27156450]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:16618965]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:16618965]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:7926742]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:7926742]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380]
Subunit of RNase MRP, nuclear RNase P and telomerase complexes; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; binds to the RPR1 RNA subunit in RNase P
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:8706758]
EXISTENCE:direct assay:GO:0004019 adenylosuccinate synthase activity [PMID:8706758|PMID:10417315]
EXISTENCE:mutant phenotype:GO:0004019 adenylosuccinate synthase activity [PMID:5807803]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:5807803]
Adenylosuccinate synthase; catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
dolichyl-P-Man:Man(6)GlcNAc(2)-PP-dolichol alpha-1,2-mannosyltransferase
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:15987956]
EXISTENCE:direct assay:GO:0052918 dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity [PMID:15987956]
EXISTENCE:direct assay:GO:0052926 dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity [PMID:15987956]
EXISTENCE:mutant phenotype:GO:0000030 mannosyltransferase activity [PMID:8692962]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:8692962]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:8692962]
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:15987956]
Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:16251400]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:11459965|PMID:16251400]
EXISTENCE:direct assay:GO:0033567 DNA replication, Okazaki fragment processing [PMID:16251400]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:32748509]
EXISTENCE:direct assay:GO:1905493 regulation of G-quadruplex DNA binding [PMID:32748509]
EXISTENCE:genetic interaction:GO:0006282 regulation of DNA repair [PMID:16809783]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:32748509]
Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
putative serine/threonine-protein phosphatase
EXISTENCE:direct assay:GO:0000298 endopolyphosphatase activity [PMID:31175919|PMID:28302909]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:14562095]
EXISTENCE:direct assay:GO:0005775 vacuolar lumen [PMID:28302909]
EXISTENCE:direct assay:GO:0006798 polyphosphate catabolic process [PMID:28302909]
EXISTENCE:genetic interaction:GO:0000298 endopolyphosphatase activity [PMID:28302909]
EXISTENCE:genetic interaction:GO:0006798 polyphosphate catabolic process [PMID:28302909]
EXISTENCE:mutant phenotype:GO:0000298 endopolyphosphatase activity [PMID:28302909]
EXISTENCE:mutant phenotype:GO:0006798 polyphosphate catabolic process [PMID:28302909]
Zn2+-dependent endopolyphosphatase; required with PPN1 to mobilize polyphosphate stores in response to phosphate starvation; member of the PPP-superfamily of metalloproteases; localizes to the vacuolar lumen via the MVB pathway; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11455386]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:16956377|PMID:26399229|PMID:9710643]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:1904543]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:12051841|PMID:10487921]
EXISTENCE:direct assay:GO:0001094 TFIID-class transcription factor complex binding [PMID:17074814]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:18195009|PMID:20559389]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2656680]
EXISTENCE:direct assay:GO:0010833 telomere maintenance via telomere lengthening [PMID:9020083]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:18195009]
EXISTENCE:direct assay:GO:0031492 nucleosomal DNA binding [PMID:19782036]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:8510148|PMID:17656141|PMID:2225074]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:31742863]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:8194531]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:20559389]
EXISTENCE:direct assay:GO:0061849 telomeric G-quadruplex DNA binding [PMID:8052638]
EXISTENCE:direct assay:GO:0070187 shelterin complex [PMID:23746845]
EXISTENCE:direct assay:GO:0071919 G-quadruplex DNA formation [PMID:8052638]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:7958893]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:7958893]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15300680]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:2010087]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:2237406]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:27122604]
EXISTENCE:mutant phenotype:GO:0010833 telomere maintenance via telomere lengthening [PMID:9020083]
EXISTENCE:mutant phenotype:GO:0031848 protection from non-homologous end joining at telomere [PMID:16096640]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:2010087]
EXISTENCE:physical interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:12080091]
EXISTENCE:physical interaction:GO:0070200 establishment of protein localization to telomere [PMID:11689698]
EXISTENCE:physical interaction:GO:0071169 establishment of protein localization to chromatin [PMID:11689698]
Essential DNA-binding transcription regulator that binds many loci; involved in transcription activation, repression, chromatin silencing, telomere length maintenance; represses divergent noncoding transcription at highly expressed genes, prevents transcription initiation at cryptic promoters near its binding sites; relocalizes to cytosol under hypoxia; recruits Sir complex to telomeric DNA; present in quiescent cell telomere hyperclusters
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:9425153]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831|PMID:12667447]
EXISTENCE:mutant phenotype:GO:0005778 peroxisomal membrane [PMID:9425153]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12667447|PMID:16679311|PMID:9425153]
EXISTENCE:physical interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:16679311]
Membrane peroxin of the peroxisomal importomer complex; complex facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:19751518]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:10029995]
hypothetical protein; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Cytoplasmic hypothetical protein; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth; contains two PH-like domains
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
ARS1413; Strong origin of replication activated in late S phase
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:10983980]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7891689]
EXISTENCE:direct assay:GO:1990446 U1 snRNP binding [PMID:10983980]
EXISTENCE:genetic interaction:GO:0007131 reciprocal meiotic recombination [PMID:2659434]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:2659434]
EXISTENCE:mutant phenotype:GO:0048026 positive regulation of mRNA splicing, via spliceosome [PMID:10983980]
mRNA-binding protein required for meiosis-specific mRNA splicing; required for chromosome pairing and meiotic recombination; Mer1p regulon embraces four essential meiotic pre-mRNAs: REC107, HFM1, AMA1 and SPO22
EXISTENCE:direct assay:GO:0005844 polysome [PMID:1394434|PMID:16413483]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9860955]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9670014]
EXISTENCE:direct assay:GO:0051083 'de novo' cotranslational protein folding [PMID:9670014]
EXISTENCE:genetic interaction:GO:0000054 ribosomal subunit export from nucleus [PMID:20368619]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:20368619]
EXISTENCE:genetic interaction:GO:0042149 cellular response to glucose starvation [PMID:19723765]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:1394434]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:15456889]
EXISTENCE:mutant phenotype:GO:0006452 translational frameshifting [PMID:16607023]
EXISTENCE:physical interaction:GO:0002181 cytoplasmic translation [PMID:1394434]
Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
hypothetical protein; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:12690111]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12690111|PMID:12612080]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15167894]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12612080]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12690111|PMID:12612080|PMID:15167894]
EXISTENCE:mutant phenotype:GO:0046830 positive regulation of RNA import into nucleus [PMID:12612080]
Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:20007951]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:16157874|PMID:18662540|PMID:20007951]
EXISTENCE:genetic interaction:GO:0006335 DNA replication-dependent chromatin assembly [PMID:18662540]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:18708354]
EXISTENCE:genetic interaction:GO:0031507 heterochromatin formation [PMID:17410207|PMID:20007951]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19683497]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:20007951]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:18708354]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:18708354]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:17410207]
EXISTENCE:mutant phenotype:GO:0042393 histone binding [PMID:20007951]
EXISTENCE:physical interaction:GO:0042393 histone binding [PMID:16157874]
Histone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1)
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:7969036]
hypothetical protein, contains a putative zinc-binding domain; expressed during sporulation; SPS18 has a paralog, GCS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:9268358]
EXISTENCE:direct assay:GO:0008670 2,4-dienoyl-CoA reductase (NADPH) activity [PMID:9268358]
EXISTENCE:direct assay:GO:0009062 fatty acid catabolic process [PMID:9268358]
Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:20951350]
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:17517611]
EXISTENCE:direct assay:GO:0030289 protein phosphatase 4 complex [PMID:16299494|PMID:17517611]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:15598824]
EXISTENCE:mutant phenotype:GO:0030289 protein phosphatase 4 complex [PMID:16299494]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:20951350]
EXISTENCE:mutant phenotype:GO:1902660 negative regulation of glucose mediated signaling pathway [PMID:24277933]
EXISTENCE:mutant phenotype:GO:2000002 negative regulation of DNA damage checkpoint [PMID:16299494|PMID:17517611]
EXISTENCE:mutant phenotype:GO:2001034 positive regulation of double-strand break repair via nonhomologous end joining [PMID:24498054]
Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
EXISTENCE:direct assay:GO:0046496 nicotinamide nucleotide metabolic process [PMID:21994945]
EXISTENCE:direct assay:GO:0052856 NADHX epimerase activity [PMID:21994945]
NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27190003]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15817685]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15817685]
EXISTENCE:direct assay:GO:0060196 positive regulation of antisense RNA transcription [PMID:27190003]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11333224]
EXISTENCE:genetic interaction:GO:0003712 transcription coregulator activity [PMID:11333224]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10455229|PMID:15789351|PMID:27190003|PMID:9335590|PM ID:11333224|PMID:2247062]
EXISTENCE:mutant phenotype:GO:0060196 positive regulation of antisense RNA transcription [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:11333224]
Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p
EXISTENCE:direct assay:GO:0000932 P-body [PMID:24386402]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11691832|PMID:24386402]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11691832]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:35085498]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:24386330|PMID:24386402|PMID:26777405]
EXISTENCE:genetic interaction:GO:0007089 traversing start control point of mitotic cell cycle [PMID:11691832]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:14685274]
EXISTENCE:mutant phenotype:GO:0003729 mRNA binding [PMID:11691832]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:11691832]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:14685274|PMID:9055077]
EXISTENCE:mutant phenotype:GO:0061013 regulation of mRNA catabolic process [PMID:24386330]
EXISTENCE:mutant phenotype:GO:0061157 mRNA destabilization [PMID:24386402]
RNA binding protein that modulates mRNA stability; regulates cell cycle, sister chromatid cohesion, and stress response genes; modulates stability and translational efficiency of CLN3 mRNA; colocalizes with P-bodies/stress granules to regulate target mRNAs; regulates cell fate and critical cell size for Start passage; regulates ploidy; self-templating mnemon that forms inactive super-assemblies, preventing G1 arrest in mother cells after unsuccessful mating and causing sterility in old cells
EXISTENCE:direct assay:GO:0036396 RNA N6-methyladenosine methyltransferase complex [PMID:22685417]
EXISTENCE:genetic interaction:GO:0080009 mRNA methylation [PMID:22685417]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:22685417]
EXISTENCE:mutant phenotype:GO:0080009 mRNA methylation [PMID:22685417]
EXISTENCE:mutant phenotype:GO:2000221 negative regulation of pseudohyphal growth [PMID:22685417]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:22685417]
EXISTENCE:physical interaction:GO:0036396 RNA N6-methyladenosine methyltransferase complex [PMID:22685417]
Sporulation-specific protein with a leucine zipper motif; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation
hypothetical protein; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YNL195C has a paralog, HBT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11784867]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11784867]
Integral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication
hypothetical protein; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
EXISTENCE:direct assay:GO:0004100 chitin synthase activity [PMID:7952171]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8970154]
EXISTENCE:direct assay:GO:0045009 chitosome [PMID:8970154]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:2523889]
Chitin synthase I; requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17179087|PMID:14562095]
EXISTENCE:direct assay:GO:0006751 glutathione catabolic process [PMID:22277648]
EXISTENCE:direct assay:GO:0036374 glutathione hydrolase activity [PMID:22277648]
EXISTENCE:direct assay:GO:0061672 glutathione hydrolase complex [PMID:22277648]
EXISTENCE:genetic interaction:GO:0008233 peptidase activity [PMID:17179087]
EXISTENCE:genetic interaction:GO:0008242 omega peptidase activity [PMID:17179087]
EXISTENCE:mutant phenotype:GO:0006751 glutathione catabolic process [PMID:17179087|PMID:22277648]
Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
EXISTENCE:mutant phenotype:GO:0042631 cellular response to water deprivation [PMID:22442684]
Hydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10725229|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10725229]
EXISTENCE:direct assay:GO:0042564 NLS-dependent protein nuclear import complex [PMID:19141610]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:19046973]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:7622450]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:19141610]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:16929305]
EXISTENCE:mutant phenotype:GO:0031144 proteasome localization [PMID:25274630]
Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation
EXISTENCE:direct assay:GO:0005825 half bridge of spindle pole body [PMID:10428957]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:1607389]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:3030557]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10428957|PMID:8188750]
Protein involved in karyogamy and spindle pole body duplication; involved in karyogamy during mating; involved in centrosome separation and duplication during mitosis; localizes to the half-bridge of the spindle pole body; interacts with Spc72p during karyogamy; also interacts with Cdc31p; essential gene
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19451230]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:19451230]
Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:10490600]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10490600|PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22902402]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:15721261]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:31665643]
EXISTENCE:mutant phenotype:GO:0016578 histone deubiquitination [PMID:22829782]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:15721261|PMID:22829782|PMID:31665643]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:15721261|PMID:26587833]
EXISTENCE:mutant phenotype:GO:2001020 regulation of response to DNA damage stimulus [PMID:31665643]
Ubiquitin-specific protease, deubiquitinates Ub-protein moieties; interacts with proteins that function in rRNA production and ribosome biogenesis via its intrinsically disordered regions; stabilizes Rpa190p by deubiquitination; controls PCNA deubiquitylation; may regulate silencing by acting on Sir4p; involved in posttranscriptionally regulating Gap1p, possibly other transporters; localized to the nucleolus; null mutant phenotypes are functionally complemented by human USP36
mitochondrial 54S ribosomal protein YmL19
hypothetical protein; expressed at both mRNA and protein levels
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:19780626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17095607]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:17095607]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22118465]
EXISTENCE:mutant phenotype:GO:0006808 regulation of nitrogen utilization [PMID:11500493]
EXISTENCE:mutant phenotype:GO:0022898 regulation of transmembrane transporter activity [PMID:2125693]
EXISTENCE:mutant phenotype:GO:0045806 negative regulation of endocytosis [PMID:22118465]
EXISTENCE:mutant phenotype:GO:0090153 regulation of sphingolipid biosynthetic process [PMID:23363605]
Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis by phosphorylating Ldb19p; positively regulates activity of the three Mep ammonium transport proteins; mediates inhibitory phosphorylation of Mep2p and Par32p; TOR complex negatively regulates Npr1p activity; NPR1 has a paralog, PRR2, that arose from the whole genome duplication
chromatin-binding/pre-rRNA-processing protein IPI3
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:22421151]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15528184]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:22421151]
EXISTENCE:direct assay:GO:0097344 Rix1 complex [PMID:14759368]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:15528184]
EXISTENCE:mutant phenotype:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:22421151]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:14759368]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:22421151]
Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:14690591]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14690591|PMID:14562095|PMID:26928762]
Putative oxidoreductase; required for cell viability
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:18216266]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11591390]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18216266]
EXISTENCE:direct assay:GO:0012505 endomembrane system [PMID:18216266]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:18216266]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:18216266]
Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity; necessary for oxidant and ramped heat stress-induced cell death; ortholog of mammalian RAC1
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:11561734]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916|PMID:385049|PMID:18782943]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:16738661]
EXISTENCE:genetic interaction:GO:0000056 ribosomal small subunit export from nucleus [PMID:22570489]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:28223523]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:28956756]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:30465652]
EXISTENCE:physical interaction:GO:0034236 protein kinase A catalytic subunit binding [PMID:22899713]
Protein component of the small (40S) ribosomal subunit; has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; nascent Rps3p is bound by specific chaperone Yar1p during translation; homologous to mammalian ribosomal protein S3 and bacterial S3
mitochondrial 54S ribosomal protein YmL22
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Cell cycle-regulated gene of unknown function; promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele; TDA7 has a paralog, YDL211C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11452019|PMID:22842922]
EXISTENCE:direct assay:GO:0030684 preribosome [PMID:17922018]
Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8914522|PMID:22842922]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8914522]
Plasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0007091 metaphase/anaphase transition of mitotic cell cycle [PMID:8895471]
EXISTENCE:mutant phenotype:GO:0010458 exit from mitosis [PMID:8895471]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473|PMID:8895471]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
EXISTENCE:direct assay:GO:0004609 phosphatidylserine decarboxylase activity [PMID:23124206]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:23124206]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:34818062]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:6427211]
EXISTENCE:direct assay:GO:0016540 protein autoprocessing [PMID:25829489]
EXISTENCE:direct assay:GO:0140042 lipid droplet formation [PMID:34818062]
EXISTENCE:mutant phenotype:GO:0004609 phosphatidylserine decarboxylase activity [PMID:8407984]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:30926815]
EXISTENCE:mutant phenotype:GO:0010636 positive regulation of mitochondrial fusion [PMID:23045528]
EXISTENCE:mutant phenotype:GO:0010954 positive regulation of protein processing [PMID:23045528]
Phosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology; partly exposed to the mitochondrial intermembrane space; ER-localized fraction is involved in formation of lipid droplets; autocatalytically processed
hypothetical protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11113177|PMID:11500510|PMID:12086627|PMID:1437546|PM ID:1448073|PMID:9858577]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:11113177|PMID:1437546|PMID:1448073|PMID:9858577]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:12086627]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11500510]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11500510]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12086627]
EXISTENCE:direct assay:GO:0051019 mitogen-activated protein kinase binding [PMID:11230135]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11230135|PMID:11500510]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9858577]
EXISTENCE:genetic interaction:GO:0003714 transcription corepressor activity [PMID:11230135]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11113177|PMID:11230135]
EXISTENCE:genetic interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11230135]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10825197|PMID:11113177|PMID:11230135|PMID:1437546|PM ID:1448073|PMID:9858577]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11113177]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:12086627]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:11113177|PMID:1437546|PMID:1448073|PMID:9858577]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:12086627]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:11230135]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11113177|PMID:11230135|PMID:12086627|PMID:10825197]
EXISTENCE:mutant phenotype:GO:0051019 mitogen-activated protein kinase binding [PMID:12086627]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11230135]
Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses
ARS1415; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:12215547]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12215547|PMID:22842922]
EXISTENCE:genetic interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:25971666]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:12215547]
EXISTENCE:mutant phenotype:GO:0072741 protein localization to cell division site [PMID:21173112]
EXISTENCE:physical interaction:GO:0032035 myosin II tail binding [PMID:21173112]
Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis
hypothetical protein; YNL165W is not an essential gene
ARS1416; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:genetic interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:12072457]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:12072457]
Component of the BUB2-dependent spindle checkpoint pathway; interacts with Bfa1p and functions upstream of Bub2p and Bfa1p
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11779510]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11779510]
EXISTENCE:mutant phenotype:GO:0042256 cytosolic ribosome assembly [PMID:11779510]
Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
hypothetical protein; identified by homology
Ribosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10970846]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:18715118]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11747810|PMID:12196508]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10970846|PMID:11914276]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12196508]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10970846|PMID:11747810|PMID:22842922]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:10970846]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:11747810|PMID:19053807]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:11259593|PMID:12972564]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:19962308]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:11747810|PMID:12196508]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:11259593|PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007163 establishment or maintenance of cell polarity [PMID:12196508]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:11259593]
EXISTENCE:mutant phenotype:GO:0050708 regulation of protein secretion [PMID:30455324]
Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:19129178]
EXISTENCE:direct assay:GO:0070726 cell wall assembly [PMID:10234784]
Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; protein demonstrates amyloid properties in vivo; YGP1 has a paralog, SPS100, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:17085444]
EXISTENCE:direct assay:GO:0097658 Asi complex [PMID:25236469]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11454748]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25236469]
EXISTENCE:mutant phenotype:GO:0036369 transcription factor catabolic process [PMID:25253722]
EXISTENCE:mutant phenotype:GO:0071230 cellular response to amino acid stimulus [PMID:17085444]
Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and inner for ubiquitin-mediated degradation; acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17615295]
EXISTENCE:genetic interaction:GO:0000030 mannosyltransferase activity [PMID:17615295]
EXISTENCE:genetic interaction:GO:0031501 mannosyltransferase complex [PMID:17615295]
EXISTENCE:mutant phenotype:GO:0000030 mannosyltransferase activity [PMID:17615295]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:17615295]
EXISTENCE:physical interaction:GO:0031501 mannosyltransferase complex [PMID:17615295]
Essential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes
EXISTENCE:direct assay:GO:0000932 P-body [PMID:20471941]
EXISTENCE:direct assay:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:23273919|PMID:23861665]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20471941|PMID:14562095]
EXISTENCE:direct assay:GO:1905183 negative regulation of protein serine/threonine phosphatase activity [PMID:23273919|PMID:23861665]
EXISTENCE:genetic interaction:GO:0048255 mRNA stabilization [PMID:20471941]
EXISTENCE:genetic interaction:GO:1900152 negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:20471941]
EXISTENCE:genetic interaction:GO:1901992 positive regulation of mitotic cell cycle phase transition [PMID:23861665]
EXISTENCE:genetic interaction:GO:1903452 positive regulation of G1 to G0 transition [PMID:20471941|PMID:23273919]
EXISTENCE:genetic interaction:GO:1905184 positive regulation of protein serine/threonine phosphatase activity [PMID:23861665]
EXISTENCE:mutant phenotype:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:23273919]
EXISTENCE:mutant phenotype:GO:1905183 negative regulation of protein serine/threonine phosphatase activity [PMID:23273919]
Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0016126 sterol biosynthetic process [PMID:16270032]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:16270032]
Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:24121501|PMID:24297164]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:24121501]
EXISTENCE:genetic interaction:GO:0071243 cellular response to arsenic-containing substance [PMID:24297164]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:24121501]
EXISTENCE:mutant phenotype:GO:0071243 cellular response to arsenic-containing substance [PMID:24297164]
EXISTENCE:physical interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:24121501]
Protein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8474447]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:1627830|PMID:8034689|PMID:16622836]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10198058]
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:10198058]
EXISTENCE:genetic interaction:GO:0000902 cell morphogenesis [PMID:8474447]
EXISTENCE:genetic interaction:GO:0010255 glucose mediated signaling pathway [PMID:27630263]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10198058]
EXISTENCE:mutant phenotype:GO:0009749 response to glucose [PMID:14755054]
Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication
tubulin-binding prefolding complex subunit GIM3
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9463374]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:9463374]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:27448207]
EXISTENCE:physical interaction:GO:0016272 prefoldin complex [PMID:9463374]
Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:18344988]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:22956544]
EXISTENCE:genetic interaction:GO:1990344 secondary cell septum biogenesis [PMID:19528296]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:18344988|PMID:22956544]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18439903]
EXISTENCE:mutant phenotype:GO:1990344 secondary cell septum biogenesis [PMID:19528296]
EXISTENCE:physical interaction:GO:0044697 HICS complex [PMID:22623719]
Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
DNA-directed RNA polymerase III subunit C31
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:mutant phenotype:GO:0006384 transcription initiation at RNA polymerase III promoter [PMID:7835345]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:2201900]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:14690591|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0015031 protein transport [PMID:16943325]
Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0007023 post-chaperonin tubulin folding pathway [PMID:9885248]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:9885248]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9885248]
EXISTENCE:mutant phenotype:GO:0007023 post-chaperonin tubulin folding pathway [PMID:9885248]
EXISTENCE:physical interaction:GO:0043014 alpha-tubulin binding [PMID:9885248]
Alpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10369684]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15075370|PMID:18029398]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18029398|PMID:22842922]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10449419]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:11333229|PMID:10428970]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:10747033]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:19806183]
EXISTENCE:physical interaction:GO:0005732 sno(s)RNA-containing ribonucleoprotein complex [PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:2046670]
EXISTENCE:direct assay:GO:0006113 fermentation [PMID:29425293]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3285180]
EXISTENCE:mutant phenotype:GO:0000772 mating pheromone activity [PMID:3285180]
Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
hypothetical protein; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; YNL144C has a paralog, YHR131C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11069679|PMID:24124599]
EXISTENCE:direct assay:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9482721]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:9234685]
EXISTENCE:mutant phenotype:GO:1900430 positive regulation of filamentous growth of a population of unicellular organisms [PMID:32069286]
Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p; Twin-His motif ensures transport specificity
EXISTENCE:direct assay:GO:0000034 adenine deaminase activity [PMID:1577682]
EXISTENCE:direct assay:GO:0006146 adenine catabolic process [PMID:1577682]
EXISTENCE:direct assay:GO:0043101 purine-containing compound salvage [PMID:1577682]
EXISTENCE:direct assay:GO:0043103 hypoxanthine salvage [PMID:1577682]
EXISTENCE:mutant phenotype:GO:0000034 adenine deaminase activity [PMID:1577682]
EXISTENCE:mutant phenotype:GO:0006146 adenine catabolic process [PMID:1577682]
EXISTENCE:mutant phenotype:GO:0043101 purine-containing compound salvage [PMID:1577682]
EXISTENCE:mutant phenotype:GO:0043103 hypoxanthine salvage [PMID:1577682]
Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
hypothetical protein; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
EXISTENCE:direct assay:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:24084588]
EXISTENCE:direct assay:GO:0003676 nucleic acid binding [PMID:12093753]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11139493|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000446 nucleoplasmic THO complex [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17537816]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12093753|PMID:17960421]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:18614048]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:27251550]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:26663077]
EXISTENCE:mutant phenotype:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:26663077]
Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:16314500|PMID:15565172]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:16314500]
Component of the SF3b subcomplex of the U2 snRNP; essential protein required for splicing and for assembly of SF3b
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:19201756]
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:19201756]
EXISTENCE:direct assay:GO:0008179 adenylate cyclase binding [PMID:2184942]
EXISTENCE:direct assay:GO:0030042 actin filament depolymerization [PMID:26458246]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:8552082]
EXISTENCE:direct assay:GO:0030836 positive regulation of actin filament depolymerization [PMID:26458246]
EXISTENCE:direct assay:GO:0051014 actin filament severing [PMID:23135996]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:19201756]
EXISTENCE:mutant phenotype:GO:0007265 Ras protein signal transduction [PMID:9774417]
EXISTENCE:mutant phenotype:GO:0030833 regulation of actin filament polymerization [PMID:30639852]
EXISTENCE:mutant phenotype:GO:0090141 positive regulation of mitochondrial fission [PMID:30639852]
CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; N- and C-termini can function as physically separate proteins; mCAP1 is the mouse homolog
mitochondrial 37S ribosomal protein NAM9
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal component of the small subunit
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15353583]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:15353583]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:15353583]
Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:1996117]
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:36113412]
EXISTENCE:direct assay:GO:0005527 macrolide binding [PMID:8325502]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:36113412|PMID:14562095]
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:34534579]
EXISTENCE:direct assay:GO:0070651 nonfunctional rRNA decay [PMID:36113412]
EXISTENCE:direct assay:GO:1903644 regulation of chaperone-mediated protein folding [PMID:34534579]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:10049913]
EXISTENCE:genetic interaction:GO:1901710 regulation of homoserine biosynthetic process [PMID:15470257]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:32603360]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:32603360]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9362068]
Peptidyl-prolyl cis-trans isomerase (PPIase); binds to FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; associates with promoters of ribosomal protein genes; acts as chaperone to prevent protein aggregation; N-terminally propionylated in vivo; mutation is functionally complemented by human FKBP1A
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:25656244]
EXISTENCE:mutant phenotype:GO:1901426 response to furfural [PMID:25656244]
NADH-dependent aldehyde reductase; involved in detoxification of furfural; expression is up-regulated in the presence of furfural and 5-hydroxymethylfurfural, which are compounds generated during lignocellulosic biomass pre-treatment; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
hypothetical protein; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:28542199]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:28542199]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:15167894]
EXISTENCE:direct assay:GO:1990883 rRNA cytidine N-acetyltransferase activity [PMID:25086048]
EXISTENCE:mutant phenotype:GO:0016556 mRNA modification [PMID:31243956]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:12150911|PMID:19806183]
Acetyltransferase required for biogenesis of small ribosomal subunit; conserved 18S rRNA cytosine acetyltransferase that modifies tRNAs assisted by adaptor Tan1p; responsible for incorporation of N4-acetylcytidine into mRNAs; human homolog NAT10 implicated in several types of cancer and premature aging
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:16407407]
EXISTENCE:direct assay:GO:0005742 mitochondrial outer membrane translocase complex [PMID:9774667]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:10519552]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:10519552|PMID:9774667]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:12628251]
Component of the TOM (Translocase of Outer Membrane) complex; responsible for initial import of mitochondrially directed proteins; mediates interaction between TOM and TIM complexes and acts as receptor for precursor proteins; implicated in mitochondrial accumulation of amyloid beta peptides via direct interaction with amyloid beta peptide residues 25-42 followed by transfer to Tom40p and transport through the TOM channel
diacylglycerol cholinephosphotransferase
EXISTENCE:direct assay:GO:0004142 diacylglycerol cholinephosphotransferase activity [PMID:3029130]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:3005242]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8543066]
EXISTENCE:direct assay:GO:0006657 CDP-choline pathway [PMID:3029130]
EXISTENCE:mutant phenotype:GO:0004142 diacylglycerol cholinephosphotransferase activity [PMID:3029130]
EXISTENCE:mutant phenotype:GO:0006657 CDP-choline pathway [PMID:3029130]
Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
hypothetical protein; dgr1 null mutant is resistant to 2-deoxy-D-glucose
EXISTENCE:direct assay:GO:0046495 nicotinamide riboside metabolic process [PMID:15137942]
EXISTENCE:direct assay:GO:0050262 ribosylnicotinamide kinase activity [PMID:15137942]
EXISTENCE:genetic interaction:GO:0009435 NAD biosynthetic process [PMID:17914902]
EXISTENCE:genetic interaction:GO:0034356 NAD biosynthesis via nicotinamide riboside salvage pathway [PMID:15137942]
EXISTENCE:genetic interaction:GO:0046495 nicotinamide riboside metabolic process [PMID:15137942]
Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
putative phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15913452]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:11070083]
PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:22782902]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23625923]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:22782902]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:22782902]
Protein involved in recovery from cell cycle arrest; acts in response to pheromone; also involved in regulation of intra-S DNA damage checkpoint and autophagy; is essential for dephosphorylation of Atg13p; interacts with Far3p, Far7p, Far8p, Far9p, Far10p and with the phosphatases Pph21p, Pph22p and Pph3p; has similarity to the N- and C-termini of N. crassa HAM-2; similar to human Fam40A and Fam40B
EXISTENCE:direct assay:GO:0005822 inner plaque of spindle pole body [PMID:9215630]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:9215630]
EXISTENCE:direct assay:GO:0007020 microtubule nucleation [PMID:11950928|PMID:24842996]
EXISTENCE:direct assay:GO:0008275 gamma-tubulin small complex [PMID:25599398|PMID:9384578|PMID:9130700]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:9153752]
EXISTENCE:mutant phenotype:GO:0043015 gamma-tubulin binding [PMID:8670895]
EXISTENCE:physical interaction:GO:0008275 gamma-tubulin small complex [PMID:9130700]
EXISTENCE:physical interaction:GO:0043015 gamma-tubulin binding [PMID:8670895|PMID:9130700]
Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11536335]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
Protein with similarity to monocarboxylate permeases; appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12515383]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:12242285|PMID:12515383]
EXISTENCE:mutant phenotype:GO:0000493 box H/ACA snoRNP assembly [PMID:12228251|PMID:12242285|PMID:12515383]
EXISTENCE:physical interaction:GO:0000493 box H/ACA snoRNP assembly [PMID:12228251|PMID:12515383]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:12515383]
RNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14657274|PMID:14562095]
EXISTENCE:direct assay:GO:0008236 serine-type peptidase activity [PMID:18946088]
EXISTENCE:mutant phenotype:GO:0004252 serine-type endopeptidase activity [PMID:29861160]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:14657274|PMID:16470384|PMID:16608876]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:18946088]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:18946088]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:16470384]
EXISTENCE:mutant phenotype:GO:0051438 regulation of ubiquitin-protein transferase activity [PMID:29861160]
EXISTENCE:mutant phenotype:GO:0120174 stress-induced homeostatically regulated protein degradation pathway [PMID:29861160]
EXISTENCE:physical interaction:GO:0044255 cellular lipid metabolic process [PMID:16470384]
Serine protease and general molecular chaperone; cleaves Roq1p, which modifies the substrate specificity of the Ubr1p Ub-ligase, promoting the stress-induced homeostatically-regulated protein degradation (SHRED) of misfolded and native ER-membrane and cytosolic proteins; chaperone activity involved in the heat stress response; promotes apoptosis through proteolysis of Bir1p; role in lipid homeostasis; mammalian Omi/HtrA2 serine protease family member
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:24675956]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:24675956]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:24675956]
Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L35 and human MRPL35 ribosomal proteins
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:2177474]
EXISTENCE:genetic interaction:GO:0006626 protein targeting to mitochondrion [PMID:18007655]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:2177474]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:8132642]
EXISTENCE:mutant phenotype:GO:0030943 mitochondrion targeting sequence binding [PMID:11054285]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:2177474]
Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially-directed proteins, acting as a receptor for incoming precursor proteins; moonlighting role as a transcriptional regulator of mitochondrial proteins; age-dependent reduction in Tom70p results in mitochondrial dysfunction, a hallmark of aging; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0002143 tRNA wobble position uridine thiolation [PMID:17018299|PMID:18755837|PMID:19151091|PMID:18664566|P MID:19145231]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:14551258]
Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
decapping enzyme complex catalytic subunit
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:10508173|PMID:11139489]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23509072|PMID:12730603]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23706738]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11139489]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23706738|PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0016787 hydrolase activity [PMID:12486012]
EXISTENCE:direct assay:GO:0050072 m7G(5')pppN diphosphatase activity [PMID:16341225]
EXISTENCE:direct assay:GO:0098562 cytoplasmic side of membrane [PMID:27146487]
EXISTENCE:direct assay:GO:0098745 Dcp1-Dcp2 complex [PMID:12554866]
EXISTENCE:genetic interaction:GO:0034063 stress granule assembly [PMID:20513766]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:10508173]
EXISTENCE:mutant phenotype:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:15225544]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:20513766]
EXISTENCE:mutant phenotype:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:23706738]
EXISTENCE:physical interaction:GO:0098745 Dcp1-Dcp2 complex [PMID:10508173]
Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex; removes 5' cap structure from mRNAs prior to their degradation; also enters nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress; human homolog DCP2 complements yeast dcp2 thermosensitive mutant
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:2211514|PMID:27678487]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:11846793]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11846793|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0004474 malate synthase activity [PMID:8878673|PMID:23642236]
EXISTENCE:mutant phenotype:GO:0006097 glyoxylate cycle [PMID:8878673|PMID:23642236]
Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:18202552]
EXISTENCE:direct assay:GO:0051865 protein autoubiquitination [PMID:18202552]
EXISTENCE:genetic interaction:GO:0000132 establishment of mitotic spindle orientation [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0000921 septin ring assembly [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:21562220]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0032186 cellular bud neck septin ring organization [PMID:22570619]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:22570619]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:18202552]
EXISTENCE:mutant phenotype:GO:0005934 cellular bud tip [PMID:23442799]
EXISTENCE:mutant phenotype:GO:0032177 cellular bud neck split septin rings [PMID:23442799]
EXISTENCE:mutant phenotype:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:27449057]
EXISTENCE:physical interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:27449057]
Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
ARS1417; Autonomously Replicating Sequence
DNA-directed RNA polymerase core subunit RPC19
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:9837969|PMID:11486042]
RNA polymerase subunit AC19; common to RNA polymerases I and III
DEAD-box ATP-dependent RNA helicase DBP2
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:23721653]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:30902808]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22679025|PMID:14562095]
EXISTENCE:direct assay:GO:0006369 termination of RNA polymerase II transcription [PMID:30902808]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:22679025]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:30902808]
EXISTENCE:genetic interaction:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:22679025]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11585918]
EXISTENCE:mutant phenotype:GO:0008186 ATP-dependent activity, acting on RNA [PMID:22679025]
EXISTENCE:mutant phenotype:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:22679025]
EXISTENCE:mutant phenotype:GO:1990120 messenger ribonucleoprotein complex assembly [PMID:23721653]
EXISTENCE:physical interaction:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:7883168]
ATP-dependent RNA helicase of the DEAD-box protein family; has strong preference for dsRNA; remodels RNA-protein complex (RNP) to facilitate efficient transcription termination; required for assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12514182]
EXISTENCE:direct assay:GO:0009055 electron transfer activity [PMID:10622712]
EXISTENCE:direct assay:GO:0016126 sterol biosynthetic process [PMID:10622712]
Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
rRNA-binding ribosome biosynthesis protein NOP15
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10953080]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11583614]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21811236]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
EXISTENCE:direct assay:GO:0016311 dephosphorylation [PMID:23569204]
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:23569204]
Protein phosphatase; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:15485911]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15302830]
Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain
phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP52
EXISTENCE:direct assay:GO:0004438 phosphatidylinositol-3-phosphate phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11094088]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:15798181|PMID:11094088]
EXISTENCE:direct assay:GO:0043812 phosphatidylinositol-4-phosphate phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0043813 phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity [PMID:10224048]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10224048]
EXISTENCE:mutant phenotype:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:9438131]
EXISTENCE:mutant phenotype:GO:0046856 phosphatidylinositol dephosphorylation [PMID:9438131]
EXISTENCE:physical interaction:GO:0030479 actin cortical patch [PMID:15798181]
Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003852 2-isopropylmalate synthase activity [PMID:25841022|PMID:3275644]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:3275644|PMID:11914276|PMID:14562095]
EXISTENCE:genetic interaction:GO:0009098 leucine biosynthetic process [PMID:10790691]
EXISTENCE:mutant phenotype:GO:0009098 leucine biosynthetic process [PMID:25841022]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:25841022]
Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:9799240]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9799240]
EXISTENCE:direct assay:GO:0046685 response to arsenic-containing substance [PMID:15689486]
EXISTENCE:direct assay:GO:0046686 response to cadmium ion [PMID:15689486]
EXISTENCE:direct assay:GO:0089713 Cbf1-Met4-Met28 complex [PMID:8665859|PMID:9171357]
EXISTENCE:mutant phenotype:GO:0000096 sulfur amino acid metabolic process [PMID:1549123]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22696683]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:9799240]
Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p
DNA-directed DNA polymerase alpha catalytic subunit POL1
EXISTENCE:curator inference:GO:0006270 DNA replication initiation [PMID:3888995]
EXISTENCE:curator inference:GO:0006273 lagging strand elongation [PMID:3888995]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:1704371|PMID:3888995|PMID:3907855]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:15773893]
EXISTENCE:direct assay:GO:0005658 alpha DNA polymerase:primase complex [PMID:3888995|PMID:22593576]
EXISTENCE:direct assay:GO:0006278 RNA-templated DNA biosynthetic process [PMID:17429354]
EXISTENCE:mutant phenotype:GO:0000731 DNA synthesis involved in DNA repair [PMID:2651896]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:2651896]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:2651896]
Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11274162]
EXISTENCE:genetic interaction:GO:0005302 L-tyrosine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0015186 L-glutamine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0015188 L-isoleucine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0032974 amino acid transmembrane export from vacuole [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0005302 L-tyrosine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015186 L-glutamine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015188 L-isoleucine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0032974 amino acid transmembrane export from vacuole [PMID:11274162]
Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23509072]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239|PMID:14562095]
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0043933 protein-containing complex organization [PMID:26968360]
Component of the MICOS complex; MICOS (formerly MINOS or MitOS), a mitochondrial inner membrane complex that extends into the intermembrane space with a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin; orthologous to human APOO and APOOL
putative tyrosine protein phosphatase OCA1
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11408586]
Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8106517]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:6438624]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23127800]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22575457|PMID:24769239]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:22575457]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20162532|PMID:23127800|PMID:16622836|PMID:11914276]
EXISTENCE:direct assay:GO:0007190 activation of adenylate cyclase activity [PMID:8106517]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:15016820]
EXISTENCE:genetic interaction:GO:0045762 positive regulation of adenylate cyclase activity [PMID:2981630|PMID:3891097]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:12782684]
EXISTENCE:mutant phenotype:GO:0000411 positive regulation of transcription by galactose [PMID:16292676]
EXISTENCE:mutant phenotype:GO:0010603 regulation of cytoplasmic mRNA processing body assembly [PMID:21925385]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:2558958]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:15016820]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:15917658]
EXISTENCE:mutant phenotype:GO:2000222 positive regulation of pseudohyphal growth [PMID:1547504|PMID:8643578]
GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:35563734]
EXISTENCE:mutant phenotype:GO:1902986 regulation of lysine biosynthetic process via aminoadipic acid [PMID:35563734]
Peroxisomal matrix protein that regulates lysine biosynthesis; localization to peroxisomes upon lysine deprivation is dependent on targeting factor Pex5p and lysine biosynthesis enzyme Lys1p; affects abundance of Lys1p and the lysine biosynthesis pathway
EXISTENCE:direct assay:GO:0000118 histone deacetylase complex [PMID:12672825]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:17142463]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27190003|PMID:24881874]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:10805724]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:24881874]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19270272]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17210643|PMID:20398213]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:2000219 positive regulation of invasive growth in response to glucose limitation [PMID:18202364]
EXISTENCE:mutant phenotype:GO:2001159 regulation of protein localization by the Cvt pathway [PMID:24881874]
Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:genetic interaction:GO:0042254 ribosome biogenesis [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
hypothetical protein; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008195 phosphatidate phosphatase activity [PMID:23071111|PMID:23335564]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:11489916]
EXISTENCE:mutant phenotype:GO:0008195 phosphatidate phosphatase activity [PMID:23071111]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:23071111]
Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
ARS1419; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:22748138]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0005770 late endosome [PMID:11872141]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8163546]
Stress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
S-adenosylmethionine-dependent methyltransferase
EXISTENCE:direct assay:GO:0006479 protein methylation [PMID:24651469]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:24651469]
S-adenosylmethionine-dependent protein methyltransferase; capable of automethylation; member of the seven beta-strand family; YNL092W is not an essential gene
EXISTENCE:direct assay:GO:0000932 P-body [PMID:34347506]
EXISTENCE:genetic interaction:GO:0017148 negative regulation of translation [PMID:34347506]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:11816027]
Translational inhibitor and suppressor of proteasome-assembly defects; P-body component that inhibits translation, rescues proteasome base assembly defects and aggregation of regulatory particle subunits during stress when overexpressed; proposed to function to bridge the HOG and pheromone pathways with the cell wall integrity pathway in response to heat stress; interacts physically and genetically with splicing factor MSL1; large, highly-charge protein containing many disordered regions
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:10526184|PMID:11591390]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18417612]
Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity and in microtubule assembly
EXISTENCE:direct assay:GO:0000795 synaptonemal complex [PMID:1315786]
EXISTENCE:direct assay:GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity [PMID:6088500|PMID:6323017]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:6088500|PMID:22842922]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:6088500]
EXISTENCE:direct assay:GO:0097047 DNA replication termination region [PMID:20797631]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:25430478]
EXISTENCE:genetic interaction:GO:0009303 rRNA transcription [PMID:2436053]
EXISTENCE:mutant phenotype:GO:0000019 regulation of mitotic recombination [PMID:2902925]
EXISTENCE:mutant phenotype:GO:0006265 DNA topological change [PMID:31969709]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:2549254]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:9199287]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:2156624]
EXISTENCE:mutant phenotype:GO:0031055 chromatin remodeling at centromere [PMID:18701701]
EXISTENCE:mutant phenotype:GO:0097046 replication fork progression beyond termination site [PMID:20797631]
Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:22250200]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:22250200]
EXISTENCE:genetic interaction:GO:0055091 phospholipid homeostasis [PMID:35440494]
EXISTENCE:genetic interaction:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:23237950]
EXISTENCE:genetic interaction:GO:0090158 endoplasmic reticulum membrane organization [PMID:23237950]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:33205016]
Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER, the cell periphery and is enriched in bud membranes; mRNA is targeted to the bud tip
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes
EXISTENCE:direct assay:GO:0000932 P-body [PMID:24970865]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:15082763]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:18617998]
Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains
EXISTENCE:direct assay:GO:1990964 actin cytoskeleton-regulatory complex [PMID:10594004|PMID:17151356]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15944351]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:10594004]
EXISTENCE:physical interaction:GO:1990964 actin cytoskeleton-regulatory complex [PMID:17151356]
EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:15238515]
EXISTENCE:mutant phenotype:GO:0005471 ATP:ADP antiporter activity [PMID:18485069]
EXISTENCE:mutant phenotype:GO:0006839 mitochondrial transport [PMID:18485069]
EXISTENCE:mutant phenotype:GO:0015866 ADP transport [PMID:18485069]
EXISTENCE:mutant phenotype:GO:0015867 ATP transport [PMID:18485069]
ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:9368761]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:11575920]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:11575920]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:11717305]
EXISTENCE:direct assay:GO:0032135 DNA insertion or deletion binding [PMID:9368761]
EXISTENCE:direct assay:GO:0032139 dinucleotide insertion or deletion binding [PMID:9368761]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:16702432]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:10938116]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:2685551]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:11717305]
EXISTENCE:physical interaction:GO:0032389 MutLalpha complex [PMID:10570173|PMID:11237696|PMID:8066446]
ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain; required for silencing at the silent mating-type loci and telomeres; similar to E. coli MutL
putative mitochondrial 37S ribosomal protein SWS2
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:28154081|PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:28154081]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:28154081]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
Putative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2; localizes to peroxisomes in glucose-growing cells
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17187761]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:17187761]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17187761]
Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:9864365]
EXISTENCE:direct assay:GO:0003786 actin lateral binding [PMID:11457840]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:7844152]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:2649250|PMID:9864365]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:9864365]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:2649250|PMID:7844152]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0090527 actin filament reorganization [PMID:17237521]
Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:25416945|PMID:30341817]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11817649]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11817649|PMID:27385335]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:25416945]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:30341817]
EXISTENCE:direct assay:GO:0032177 cellular bud neck split septin rings [PMID:30341817]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:30341817]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:29074565]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:29074565]
EXISTENCE:physical interaction:GO:0140014 mitotic nuclear division [PMID:11817649]
Protein involved in axial bud site selection; prevents repolarization of cells at previous division sites; recruited with Nba1p to the cell cortex and to cytokinesis remnants (bud scars) by Aim44p and Nap1p where Nba1p prevents Rsr1p-mediated activation of Cdc24p, and therefore Cdc42p activation; acts with Nba1p to establish transient bud scar localization of Rga1p, a Cdc42p GAP; localizes to the nucleus and to cell division site during G2/M, and is then stably recruited to cytokinesis remnants
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:23329686]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922|PMID:14562095]
EXISTENCE:direct assay:GO:0042405 nuclear inclusion body [PMID:32492414]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:23329686]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:32492414]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:11923285]
Hsp40 chaperone with a role in SUMO-mediated protein degradation; works in concert with Hsp70 and Hsp110 (Sse1p) to promote disaggregation of intranuclear protein inclusions; competes with Hsp104 in disaggregation, supporting turnover instead of refolding; member of DnaJ-like family, conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10511541]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11882290]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11882290]
EXISTENCE:mutant phenotype:GO:0006808 regulation of nitrogen utilization [PMID:10511541]
EXISTENCE:mutant phenotype:GO:2000220 regulation of pseudohyphal growth [PMID:22043304]
EXISTENCE:physical interaction:GO:0031930 mitochondria-nucleus signaling pathway [PMID:14536080]
Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
snoRNA-binding rRNA-processing protein IMP4
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:23980203]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:15489263]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309|PMID:12242301]
EXISTENCE:direct assay:GO:0034457 Mpp10 complex [PMID:17515605]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:11864606]
EXISTENCE:direct assay:GO:0042274 ribosomal small subunit biogenesis [PMID:23980203]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17803460]
Component of the SSU processome; SSU processome is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:16816427]
EXISTENCE:genetic interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:16816427]
Serine-rich hypothetical protein; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2016746|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0008033 tRNA processing [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0004824 lysine-tRNA ligase activity [PMID:2016746]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0070154 mitochondrial lysyl-tRNA aminoacylation [PMID:2016746]
Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)
ribonuclease H2 catalytic subunit RNH201
EXISTENCE:direct assay:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11029655]
EXISTENCE:direct assay:GO:0032299 ribonuclease H2 complex [PMID:14734815]
EXISTENCE:direct assay:GO:0043137 DNA replication, removal of RNA primer [PMID:10567561]
EXISTENCE:genetic interaction:GO:0043137 DNA replication, removal of RNA primer [PMID:10567561]
EXISTENCE:mutant phenotype:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
dihydrolipoyllysine-residue acetyltransferase
EXISTENCE:direct assay:GO:0004742 dihydrolipoyllysine-residue acetyltransferase activity [PMID:2271545]
EXISTENCE:direct assay:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:2007123|PMID:9038189]
EXISTENCE:direct assay:GO:0006086 acetyl-CoA biosynthetic process from pyruvate [PMID:7947791]
Dihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA; metabolic longevity factor required for calorie restriction-mediated life span extension
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:mutant phenotype:GO:0005742 mitochondrial outer membrane translocase complex [PMID:8641278]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:8641278]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:8641278]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:8641278]
Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:27374275]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:27374275]
Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication
forkhead family transcription factor FKH2
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:24504085]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:22265405|PMID:24504085]
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:15509804|PMID:10959837]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:10899128]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10899128|PMID:20847055|PMID:22932476]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:24504085|PMID:19111667]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:10899128|PMID:10959837|PMID:12711672|PMID:15509804]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:17283050]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:12702877]
EXISTENCE:genetic interaction:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:10747051]
EXISTENCE:genetic interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:10959837|PMID:15509804]
EXISTENCE:genetic interaction:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:17898805]
EXISTENCE:genetic interaction:GO:0090282 positive regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:11562353|PMID:15509804|PMID:10747051|PMID:10894548|P MID:10959837]
EXISTENCE:genetic interaction:GO:0090419 negative regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:10894549]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:10747051|PMID:10894548]
EXISTENCE:mutant phenotype:GO:0003712 transcription coregulator activity [PMID:15509804]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:17283050]
EXISTENCE:mutant phenotype:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:22265405]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:12702877]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:10747051]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12711672|PMID:10899128|PMID:15509804]
EXISTENCE:mutant phenotype:GO:0090282 positive regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:10959837|PMID:10899128]
EXISTENCE:mutant phenotype:GO:1903468 positive regulation of DNA replication initiation [PMID:26728715]
EXISTENCE:mutant phenotype:GO:2000221 negative regulation of pseudohyphal growth [PMID:10894548]
Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia
ARS1420; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L9B; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9B has a paralog, RPL9A, that arose from a single-locus duplication
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11958935]
Cell wall protein related to glucanases localized in birth scars; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11922628|PMID:22842922|PMID:34887841]
EXISTENCE:genetic interaction:GO:0032973 amino acid export across plasma membrane [PMID:15590823]
EXISTENCE:mutant phenotype:GO:0008028 monocarboxylic acid transmembrane transporter activity [PMID:11922628]
EXISTENCE:mutant phenotype:GO:0015565 threonine efflux transmembrane transporter activity [PMID:34887841]
EXISTENCE:mutant phenotype:GO:0015718 monocarboxylic acid transport [PMID:11922628]
EXISTENCE:mutant phenotype:GO:0032973 amino acid export across plasma membrane [PMID:15590823|PMID:34887841]
EXISTENCE:mutant phenotype:GO:0042910 xenobiotic transmembrane transporter activity [PMID:11922628]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:11922628]
Plasma membrane transporter of the major facilitator superfamily; involved in the excretion of excess amino acids; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; relocalizes from plasma membrane to cytoplasm upon DNA replication stress; AQR1 has a paralog, QDR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:1400408|PMID:15342786]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:1869583|PMID:8144572|PMID:26928762]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9674429]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:1869583]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9774392]
EXISTENCE:direct assay:GO:0072380 TRC complex [PMID:20850366]
EXISTENCE:mutant phenotype:GO:0006458 'de novo' protein folding [PMID:10567418]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25344756]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:1729605]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:15252059|PMID:15342786]
EXISTENCE:mutant phenotype:GO:0035719 tRNA import into nucleus [PMID:25853343]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:1473150]
Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; acts as an adaptor that helps Rsp5p recognize cytosolic misfolded proteins for ubiquitination after heat shock; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family; chimeric protein in which human p58IPK J domain replaces yeast Ydj1p J domain can complement yeast ydj1 mutant
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:16321977]
EXISTENCE:genetic interaction:GO:0006451 translational readthrough [PMID:16321977]
EXISTENCE:mutant phenotype:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:16321977]
S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
tRNA 1-methyladenosine methyltransferase subunit GCD10
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9851972]
EXISTENCE:direct assay:GO:0016429 tRNA (adenine-N1-)-methyltransferase activity [PMID:10779558]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:10779558]
EXISTENCE:direct assay:GO:0031515 tRNA (m1A) methyltransferase complex [PMID:10779558]
EXISTENCE:physical interaction:GO:0031515 tRNA (m1A) methyltransferase complex [PMID:10779558]
Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
rRNA (cytosine-C5-)-methyltransferase NOP2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:26196125|PMID:14562095]
EXISTENCE:direct assay:GO:0009383 rRNA (cytosine-C5-)-methyltransferase activity [PMID:23913415]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:22735702|PMID:17443350]
EXISTENCE:direct assay:GO:0070475 rRNA base methylation [PMID:23913415]
EXISTENCE:genetic interaction:GO:0009383 rRNA (cytosine-C5-)-methyltransferase activity [PMID:23913415]
EXISTENCE:genetic interaction:GO:0070475 rRNA base methylation [PMID:23913415]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8972218]
EXISTENCE:mutant phenotype:GO:0009383 rRNA (cytosine-C5-)-methyltransferase activity [PMID:23913415]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:8972218]
EXISTENCE:mutant phenotype:GO:0070475 rRNA base methylation [PMID:23913415]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11011149|PMID:10923024]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:26306040]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:12887900|PMID:15525518]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:mutant phenotype:GO:0031011 Ino80 complex [PMID:12887900]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:15525518]
Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; promotes nucleosome shifts in the 3 prime direction
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to vacuole; not an essential gene; YNL058C has a paralog, PRM5, that arose from the whole genome duplication
hypothetical protein; similar to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:7499333|PMID:16407407]
EXISTENCE:direct assay:GO:0006811 ion transport [PMID:9593723]
EXISTENCE:direct assay:GO:0008308 voltage-gated anion channel activity [PMID:9593723]
EXISTENCE:direct assay:GO:0048039 ubiquinone binding [PMID:28051849]
EXISTENCE:genetic interaction:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:23104570]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17822411]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11488609]
EXISTENCE:mutant phenotype:GO:0042307 positive regulation of protein import into nucleus [PMID:29359182]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:18768136]
EXISTENCE:mutant phenotype:GO:0051027 DNA transport [PMID:19056337]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; interacts with Om45 and Om14 in the outer membrane; phosphorylated; protein abundance increases in response to DNA replication stress
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
YNLWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
TyB Gag-Pol protein; proteolytically processed to make the Gag, reverse transcriptase (RT), protease (PR), and integrase (IN) proteins that are required for retrotransposition
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:9372916|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9372916]
EXISTENCE:genetic interaction:GO:0010513 positive regulation of phosphatidylinositol biosynthetic process [PMID:11950935]
EXISTENCE:genetic interaction:GO:0033674 positive regulation of kinase activity [PMID:11950935]
EXISTENCE:mutant phenotype:GO:1903778 protein localization to vacuolar membrane [PMID:17699591]
Integral vacuolar membrane protein; involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock; targeted to vacuole via AP-3 pathway
tyrosine/serine/threonine protein phosphatase MSG5
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17952059|PMID:17963515]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17952059|PMID:17963515|PMID:14562095]
EXISTENCE:direct assay:GO:0008138 protein tyrosine/serine/threonine phosphatase activity [PMID:8306972]
EXISTENCE:direct assay:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:14703512]
EXISTENCE:direct assay:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:14703512|PMID:8306972]
EXISTENCE:genetic interaction:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:8306972]
EXISTENCE:genetic interaction:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:10625705|PMID:11923319]
EXISTENCE:genetic interaction:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:10625705|PMID:11923319]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12556475|PMID:8306972]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:14703512]
EXISTENCE:mutant phenotype:GO:0071701 regulation of MAPK export from nucleus [PMID:12556475|PMID:17963515]
EXISTENCE:mutant phenotype:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:10625705|PMID:14703512]
EXISTENCE:mutant phenotype:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:10625705|PMID:14703512|PMID:8306972]
Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
EXISTENCE:mutant phenotype:GO:0004129 cytochrome-c oxidase activity [PMID:1847916]
Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0017119 Golgi transport complex [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20065092]
Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
hypothetical protein; YNL050c is not an essential gene
EXISTENCE:direct assay:GO:0090110 COPII-coated vesicle cargo loading [PMID:10712514]
EXISTENCE:genetic interaction:GO:0090110 COPII-coated vesicle cargo loading [PMID:10712514|PMID:12941277]
EXISTENCE:physical interaction:GO:0030127 COPII vesicle coat [PMID:10749860|PMID:10753972]
Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004377 GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity [PMID:16878994]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11278778]
EXISTENCE:direct assay:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:16878994]
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:11278778]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:11278778]
Alpha-1,2-mannosyltransferase; catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER
phosphatidylinositol 4,5-bisphosphate-binding protein
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15372071|PMID:15689497]
EXISTENCE:genetic interaction:GO:0001558 regulation of cell growth [PMID:15689497]
EXISTENCE:genetic interaction:GO:0016197 endosomal transport [PMID:21451250]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:15372071]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:15689497]
EXISTENCE:genetic interaction:GO:0070941 eisosome assembly [PMID:21451250]
EXISTENCE:genetic interaction:GO:0072659 protein localization to plasma membrane [PMID:22307609]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:15689497]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:15689497]
EXISTENCE:physical interaction:GO:0031929 TOR signaling [PMID:15689497]
Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
hypothetical protein; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
bifunctional aminopeptidase/epoxide hydrolase
EXISTENCE:direct assay:GO:0004177 aminopeptidase activity [PMID:10574934]
EXISTENCE:direct assay:GO:0004301 epoxide hydrolase activity [PMID:10574934]
EXISTENCE:direct assay:GO:0030163 protein catabolic process [PMID:10574934]
EXISTENCE:direct assay:GO:0044255 cellular lipid metabolic process [PMID:10574934]
Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16002643]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:16002643]
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11157978]
Protein localized to COPII vesicles; proposed to be involved in ER-to-Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p; integral membrane protein that localizes to the ER, the Golgi apparatus, transport vesicles and the peroxisome; two isoforms exist, a full-length Yip3L (Yip3 Long) and a shorter peroxisomal matrix isoform Yip3S (Yip3 Short)
ARS1421; Autonomously Replicating Sequence
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin
EXISTENCE:mutant phenotype:GO:0071406 cellular response to methylmercury [PMID:15796894]
hypothetical protein; potential Cdc28p substrate; overproduction confers resistance to methylmercury
hypothetical protein; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene
transcription factor TFIIIB subunit BDP1
EXISTENCE:curator inference:GO:0000995 RNA polymerase III general transcription initiation factor activity [PMID:11387215|PMID:12930823|PMID:1458536]
EXISTENCE:direct assay:GO:0000126 transcription factor TFIIIB complex [PMID:7568218|PMID:1458536]
EXISTENCE:direct assay:GO:0001156 TFIIIC-class transcription factor complex binding [PMID:12930823]
EXISTENCE:direct assay:GO:0070896 positive regulation of transposon integration [PMID:10882723]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11387215]
EXISTENCE:mutant phenotype:GO:0001112 DNA-templated transcription open complex formation [PMID:11387215]
EXISTENCE:mutant phenotype:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11387215]
EXISTENCE:physical interaction:GO:0001156 TFIIIC-class transcription factor complex binding [PMID:12930823]
Essential subunit of RNA polymerase III transcription factor (TFIIIB); TFIIIB is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
phosphatidylinositol N-acetylglucosaminyltransferase GPI15
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:11746600]
EXISTENCE:mutant phenotype:GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity [PMID:11746600]
Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
EXISTENCE:direct assay:GO:0004449 isocitrate dehydrogenase (NAD+) activity [PMID:16884682]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8626605]
EXISTENCE:direct assay:GO:0005962 mitochondrial isocitrate dehydrogenase complex (NAD+) [PMID:16884682]
EXISTENCE:direct assay:GO:0006102 isocitrate metabolic process [PMID:16884682]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
EXISTENCE:direct assay:GO:0004089 carbonate dehydratase activity [PMID:15096093|PMID:15813743]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15096093]
EXISTENCE:mutant phenotype:GO:0071244 cellular response to carbon dioxide [PMID:22253597]
Carbonic anhydrase; metalloenzyme that catalyzes CO2 hydration to bicarbonate, which is an important metabolic substrate, and protons; not expressed under conditions of high CO2, such as inside a growing colony, but transcription is induced in response to low CO2 levels, such as on the colony surface in ambient air; poorly transcribed under aerobic conditions and at an undetectable level under anaerobic conditions; abundance increases in response to DNA replication stress
ARS1422; Autonomously Replicating Sequence
YNLCTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; mutated in S288C
ARS1423; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095|PMID:22842922]
Nuclear hypothetical protein; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
Protein required for sporulation; induced during meiosis and sporulation; gene is a member of Centroid, a centromere-linked gene family; not an essential gene; YNL034W has a paralog, YNL018C, that arose from a segmental duplication
hypothetical protein; YNL033W has a paralog, YNL019C, that arose from a segmental duplication
putative tyrosine protein phosphatase SIW14
EXISTENCE:direct assay:GO:0052845 inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity [PMID:26828065|PMID:29540476]
EXISTENCE:direct assay:GO:0052847 inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity [PMID:29540476]
EXISTENCE:direct assay:GO:0106211 inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity [PMID:29540476]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:15020461]
EXISTENCE:mutant phenotype:GO:0052845 inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity [PMID:26828065|PMID:29540476]
EXISTENCE:mutant phenotype:GO:0052847 inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity [PMID:29540476]
EXISTENCE:mutant phenotype:GO:0071543 diphosphoinositol polyphosphate metabolic process [PMID:26828065]
EXISTENCE:mutant phenotype:GO:0106211 inositol-5-diphosphate-1,3,4,6-tetrakisphosphate diphosphatase activity [PMID:29540476]
Inositol phosphatase involved in inositol pyrophosphate metabolism; hydrolyzes the beta-phosphate from the 5-diphosphate group of PP-InsPs including: 5PP-IP5 (IP7), 1,5PP-IP4 (IP8) and 5-PP-InsP4; functions as an anti-prion (anti-[PSI]) component; member of the atypical dual-specificity subgroup of the protein tyrosine-phosphatase (PTP) superfamily; localizes to the cytoplasm
EXISTENCE:direct assay:GO:0008823 cupric reductase activity [PMID:32631887]
EXISTENCE:direct assay:GO:0043505 CENP-A containing nucleosome [PMID:22693454]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0008823 cupric reductase activity [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0009303 rRNA transcription [PMID:16002464]
EXISTENCE:mutant phenotype:GO:0043935 sexual sporulation resulting in formation of a cellular spore [PMID:20713519]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:21460225]
Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
EXISTENCE:curator inference:GO:0097502 mannosylation [PMID:9090056]
Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR5 has a paralog, KTR7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:11523797]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11523797|PMID:20412803]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10197980]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10197980]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11523797|PMID:20412803]
EXISTENCE:direct assay:GO:0071483 cellular response to blue light [PMID:23335962]
EXISTENCE:genetic interaction:GO:0006038 cell wall chitin biosynthetic process [PMID:21343301]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20412803|PMID:25258318|PMID:9407035]
EXISTENCE:genetic interaction:GO:0050801 ion homeostasis [PMID:9407035]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20412803|PMID:15299026|PMID:9559673|PMID:9407035|PMI D:9407036|PMID:11523797]
EXISTENCE:mutant phenotype:GO:0050801 ion homeostasis [PMID:9407035]
EXISTENCE:mutant phenotype:GO:2001040 positive regulation of cellular response to drug [PMID:17056742]
Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
EXISTENCE:direct assay:GO:0001401 SAM complex [PMID:14570913]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14690591|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:14699090|PMID:16407407]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:14685243]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:18039934|PMID:14570913]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:14685243|PMID:14570913]
Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
cyclin-dependent protein serine/threonine kinase regulator SSN8
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:23447536]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:7877695]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:11383511|PMID:12200444]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:14749387]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16109375]
EXISTENCE:mutant phenotype:GO:0000411 positive regulation of transcription by galactose [PMID:15601835]
EXISTENCE:mutant phenotype:GO:0010673 positive regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:12455972]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:7732022]
Cyclin-like component of RNA polymerase II holoenzyme; involved in phosphorylation of RNAPII C-terminal domain; forms kinase-cyclin pair in RNAPII holoenzyme with Ssn3p; needed for entry into and execution of meiotic program; involved in glucose repression, telomere maintenance; degraded in response to nitrogen starvation, and also to oxidative stress, but in different cellular compartments, and with different cellular outcomes; 35% identical to human cyclin C
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:1398074]
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:8602511]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:8290578|PMID:8602511|PMID:8247008]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:8247008|PMID:8290578]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:22977255]
EXISTENCE:mutant phenotype:GO:0045787 positive regulation of cell cycle [PMID:12136008]
RNA component of RNase MRP; RNase MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; human ortholog is implicated in cartilage-hair hypoplasia (CHH)
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U2417
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:19942856]
Essential protein suggested to function early in the secretory pathway; inviability is suppressed by overexpression of Golgi protein Tvp23p; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and cytosol respectively; ortholog of human Kish
putative protein-lysine N-methyltransferase
Putative S-adenosylmethionine-dependent lysine methyltransferase; responsible for modifying Lys-390 in translational elongation factor EF-1 alpha (eEF1A); has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10998178|PMID:36113412]
Protein that acts as sensor of stalled ribosomes; binds to Fpr1p and Smu2p, and triggers ubiquitination of stalled monosomes for subsequent degradation; confers rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1
rRNA (cytosine-C5-)-methyltransferase RCM1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10649453]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:23913415]
rRNA m5C methyltransferase; methylates cytosine at position 2278 of 25S rRNA while Nop2p methylates cytosine at position 2870; contains seven beta-strand methyltransferase motif; localized to the nucleolus; interacts with Trm112p; homolog of NSUN5A, a human gene which is deleted in Williams-Beuren Syndrome
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16415367]
EXISTENCE:direct assay:GO:0004407 histone deacetylase activity [PMID:19573535]
EXISTENCE:direct assay:GO:0070823 HDA1 complex [PMID:11287668|PMID:8663039|PMID:8962081]
EXISTENCE:genetic interaction:GO:0010621 negative regulation of transcription by transcription factor localization [PMID:17974563]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11172717|PMID:17121596]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17706600]
EXISTENCE:physical interaction:GO:0070823 HDA1 complex [PMID:11287668]
Catalytic subunit of class II HDA1 histone deacetylase complex; role in azole resistance via Hsp90p, and in heat shock response; Hda1p interacts with Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; the HDA1 histone deacetylase complex regulates aging through trehalose metabolism
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:17978096]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10087264]
EXISTENCE:genetic interaction:GO:2000369 regulation of clathrin-dependent endocytosis [PMID:12956961]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:10087264]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:10087264]
EXISTENCE:mutant phenotype:GO:2000369 regulation of clathrin-dependent endocytosis [PMID:12956961]
Ser/Thr protein kinase; phosphorylates Pan1p-Sla1p-End3p protein complex subunits, Pan1p and Sla1p; involved in regulation of the cortical actin cytoskeleton and endocytosis; functional overlap with PRK1
hypothetical protein; expression induced during heat stress; YNL019C has a paralog, YNL033W, that arose from a segmental duplication
Protein required for sporulation; induced during meiosis and sporulation; gene is a member of Centroid, a centromere-linked gene family; YNL018C has a paralog, YNL034W, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:10892745]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:15964806|PMID:8413212|PMID:8413213|PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8413212]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8413212|PMID:8413213]
EXISTENCE:direct assay:GO:0008266 poly(U) RNA binding [PMID:8413213]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:24291094|PMID:26777405]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:28467675]
EXISTENCE:mutant phenotype:GO:0003729 mRNA binding [PMID:8413212|PMID:8413213]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:28467675]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:18981231]
EXISTENCE:mutant phenotype:GO:0043488 regulation of mRNA stability [PMID:15964806]
EXISTENCE:physical interaction:GO:0006415 translational termination [PMID:28467675]
Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds cell wall mRNAs; required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; ribosome-bound Pub1p modulates stop codon decoding during translation termination
EXISTENCE:direct assay:GO:0004866 endopeptidase inhibitor activity [PMID:2015812]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762|PMID:9159115]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:9159115]
EXISTENCE:mutant phenotype:GO:0030162 regulation of proteolysis [PMID:2015812]
Cytosolic inhibitor of vacuolar proteinase B (PRB1); required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9544245]
EXISTENCE:genetic interaction:GO:0003746 translation elongation factor activity [PMID:9544245]
EXISTENCE:genetic interaction:GO:0006414 translational elongation [PMID:9544245]
EXISTENCE:physical interaction:GO:0022626 cytosolic ribosome [PMID:9544245]
Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication
ARS1424; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18756268]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10855497]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17179081]
EXISTENCE:genetic interaction:GO:0032120 ascospore-type prospore membrane formation [PMID:22611022]
EXISTENCE:genetic interaction:GO:0070583 spore membrane bending pathway [PMID:18756268]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:10855497]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:22611022|PMID:26605945]
EXISTENCE:mutant phenotype:GO:0070583 spore membrane bending pathway [PMID:18756268]
Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
hypothetical protein; YNL011C is not an essential gene
EXISTENCE:direct assay:GO:0000121 glycerol-1-phosphatase activity [PMID:26071590]
EXISTENCE:direct assay:GO:0043136 glycerol-3-phosphatase activity [PMID:30240188]
EXISTENCE:direct assay:GO:0050286 sorbitol-6-phosphatase activity [PMID:30240188]
EXISTENCE:direct assay:GO:0110130 ribitol-5-phosphatase activity [PMID:30240188]
EXISTENCE:mutant phenotype:GO:0052646 alditol phosphate metabolic process [PMID:30240188]
Sugar alcohol phosphatase; polyol phosphatase that hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and (D)-glycerol-3-phosphate, maintaining phosphoglucose isomerase (PGI) activity in the presence of PGI-inhibitory sugar alcohols; expression correlated with growth rate; GFP-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant displays increased glycogen accumulation; member of the haloacid dehalogenase (HAD) superfamily
EXISTENCE:direct assay:GO:0004450 isocitrate dehydrogenase (NADP+) activity [PMID:9452501]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15001388|PMID:11914276]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:9452501|PMID:22375831]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:9452501]
Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:genetic interaction:GO:0004842 ubiquitin-protein transferase activity [PMID:11454748]
Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
ARS1425; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22718905]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:33326013]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:33293525]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:9774392]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:8513501]
EXISTENCE:direct assay:GO:0051787 misfolded protein binding [PMID:23791384|PMID:33293525]
EXISTENCE:mutant phenotype:GO:0002236 detection of misfolded protein [PMID:33293525]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:8513501]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:14657253]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:33293525]
EXISTENCE:mutant phenotype:GO:0070843 misfolded protein transport [PMID:23791384]
EXISTENCE:mutant phenotype:GO:0071630 nuclear protein quality control by the ubiquitin-proteasome system [PMID:23791384]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579]
Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12719473]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:12719473]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:12719473]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:12719473]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:12719473]
EXISTENCE:mutant phenotype:GO:0031930 mitochondria-nucleus signaling pathway [PMID:11742997]
EXISTENCE:mutant phenotype:GO:0043539 protein serine/threonine kinase activator activity [PMID:16002396]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:12408816]
EXISTENCE:physical interaction:GO:0031931 TORC1 complex [PMID:12408816]
EXISTENCE:physical interaction:GO:0031932 TORC2 complex [PMID:12408816]
Subunit of TORC1 and TORC2 involved in TOR signaling; TORC1 regulates nutrient responsive growth and TORC2 regulates plasma membrane (PM) homeostasis and cell-cycle dependent actin cytoskeleton polarization; negative regulator of Rtg1p/3p and Gln3p transcription factors and amino acid biosynthesis; binds to both Tor1p and Tor2p, regulating the integrity and kinase activity of TORC2; PM retention is dependent on Avo3p; WD-repeat protein
mitochondrial 54S ribosomal protein YmL2
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:28931599]
Mitochondrial ribosomal protein of the large subunit; involved together with Mrpl35p in assembly of cytochrome c oxidase
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:14769921]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:14676199]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640|PMID:14769921]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9499403]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9499403]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:24452287]
Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000095 S-adenosyl-L-methionine transmembrane transporter activity [PMID:14609944]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14609944|PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0015805 S-adenosyl-L-methionine transport [PMID:14609944]
EXISTENCE:genetic interaction:GO:0000095 S-adenosyl-L-methionine transmembrane transporter activity [PMID:14609944]
EXISTENCE:genetic interaction:GO:0015805 S-adenosyl-L-methionine transport [PMID:14609944]
EXISTENCE:mutant phenotype:GO:0000095 S-adenosyl-L-methionine transmembrane transporter activity [PMID:14609944]
EXISTENCE:mutant phenotype:GO:0015805 S-adenosyl-L-methionine transport [PMID:14609944]
S-adenosylmethionine transporter of the mitochondrial inner membrane; member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11087857]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:24129494]
EXISTENCE:direct assay:GO:0042273 ribosomal large subunit biogenesis [PMID:23945946]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11087857]
EXISTENCE:mutant phenotype:GO:0000465 exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11087857]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11087857]
Nucleolar protein similar to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs; binds junction of ITS2 and ITS2-proximal stem between the 3' end of 5.8S rRNA and the 5' end of 25S rRNA
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:17889667]
EXISTENCE:direct assay:GO:0032790 ribosome disassembly [PMID:20947765|PMID:24424461]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:21623367]
EXISTENCE:direct assay:GO:1990533 Dom34-Hbs1 complex [PMID:21623367]
EXISTENCE:genetic interaction:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:16554824]
EXISTENCE:mutant phenotype:GO:0032790 ribosome disassembly [PMID:22770215|PMID:24424461]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:24424461]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19481524]
EXISTENCE:mutant phenotype:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:19420139]
Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004108 citrate (Si)-synthase activity [PMID:17570335]
EXISTENCE:direct assay:GO:0046356 acetyl-CoA catabolic process [PMID:17570335]
EXISTENCE:genetic interaction:GO:0006101 citrate metabolic process [PMID:12913175]
EXISTENCE:mutant phenotype:GO:0004108 citrate (Si)-synthase activity [PMID:17570335]
EXISTENCE:mutant phenotype:GO:0046356 acetyl-CoA catabolic process [PMID:17570335]
Mitochondrial citrate synthase; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate, which is the first and rate-limiting step of the TCA cycle; transcription subject to glucose repression; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17395151]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:12429834]
Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication
DNA-directed RNA polymerase III subunit C34
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:1400451]
RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
ARS1426; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:27348091]
EXISTENCE:mutant phenotype:GO:0036261 7-methylguanosine cap hypermethylation [PMID:20621096]
Protein with a role in snRNA and snoRNA cap trimethylation; interacts with Tgs1p and shows similar phenotypes; required for trimethylation of the caps of spliceosomal snRNAs and the U3 snoRNA, and for efficient 3' end processing of U3 snoRNA; may act as a specificity factor for Tgs1p
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12055639]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:31740006]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:9702203]
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12055639|PMID:20150893]
EXISTENCE:direct assay:GO:0070530 K63-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12055639]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:31740006]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:12055639]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0006623 protein targeting to vacuole [PMID:12055639]
EXISTENCE:physical interaction:GO:0033565 ESCRT-0 complex [PMID:12055639]
Ubiquitin binding protein involved in endosomal protein sorting; K48-, K63-Ub chain binding protein that preferentially binds K63-linked chains in vitro; subunit of the ESCRT-0 complex that binds to ubiquitin via two ubiquitin-interacting motifs (UIMs) and is involved in sorting cargo into multivesicular bodies; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:25645919|PMID:25680528]
EXISTENCE:direct assay:GO:0006501 C-terminal protein lipidation [PMID:15277523]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:25645919|PMID:25680528]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:25680528]
EXISTENCE:mutant phenotype:GO:0006501 C-terminal protein lipidation [PMID:11100732|PMID:15277523]
EXISTENCE:mutant phenotype:GO:0019776 Atg8 ligase activity [PMID:11100732]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:9023185]
EXISTENCE:mutant phenotype:GO:0061908 phagophore [PMID:25680528]
E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
phospholipid:diacylglycerol acyltransferase
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:31422915]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:22454508]
EXISTENCE:direct assay:GO:0006672 ceramide metabolic process [PMID:22738231]
EXISTENCE:direct assay:GO:0019432 triglyceride biosynthetic process [PMID:10747858]
EXISTENCE:direct assay:GO:0019915 lipid storage [PMID:10747858]
EXISTENCE:direct assay:GO:0046027 phospholipid:diacylglycerol acyltransferase activity [PMID:10747858]
EXISTENCE:direct assay:GO:0055091 phospholipid homeostasis [PMID:31422915]
EXISTENCE:direct assay:GO:0097038 perinuclear endoplasmic reticulum [PMID:22454508]
EXISTENCE:mutant phenotype:GO:0003824 catalytic activity [PMID:31422915]
EXISTENCE:mutant phenotype:GO:0006672 ceramide metabolic process [PMID:22738231]
EXISTENCE:mutant phenotype:GO:0019432 triglyceride biosynthetic process [PMID:10747858]
EXISTENCE:mutant phenotype:GO:0019915 lipid storage [PMID:10747858]
Acyltransferase that converts diacylglycerol to triacylglycerol (TGA); one of several acyltransferases that contribute to TGA synthesis; relocates from ER to nuclear membrane in response cell cycle and starvation signals; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase
EXISTENCE:direct assay:GO:0030907 MBF transcription complex [PMID:16916637]
EXISTENCE:mutant phenotype:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:16916637]
EXISTENCE:physical interaction:GO:0140297 DNA-binding transcription factor binding [PMID:16916637]
Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:19077037]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:19077037]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9812975]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11470794]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:19077037]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9891034]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:8943336]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:1534753]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:2251118]
EXISTENCE:mutant phenotype:GO:0034247 snoRNA splicing [PMID:24442613]
RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16
EXISTENCE:mutant phenotype:GO:0004849 uridine kinase activity [PMID:10501935]
EXISTENCE:mutant phenotype:GO:0008655 pyrimidine-containing compound salvage [PMID:11872485]
Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:17804816]
EXISTENCE:genetic interaction:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:11779791]
EXISTENCE:genetic interaction:GO:0006817 phosphate ion transport [PMID:11779791]
EXISTENCE:genetic interaction:GO:0015169 glycerol-3-phosphate transmembrane transporter activity [PMID:20133652]
EXISTENCE:genetic interaction:GO:0015794 glycerol-3-phosphate transmembrane transport [PMID:20133652]
EXISTENCE:genetic interaction:GO:2000185 regulation of phosphate transmembrane transport [PMID:12821119]
EXISTENCE:mutant phenotype:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:12581367]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:17804816]
EXISTENCE:mutant phenotype:GO:0015169 glycerol-3-phosphate transmembrane transporter activity [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0015794 glycerol-3-phosphate transmembrane transport [PMID:20133652]
Low-affinity vacuolar phosphate transporter; exports phosphate from vacuolar lumen to cytosol; regulates phosphate and polyphosphate metabolism; acts upstream of Pho81p in regulation of PHO pathway; localizes to sites of contact between vacuole and mitochondria (vCLAMPs); targeted to vacuole via AP-3 pathway; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
hypothetical protein; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
tRNA-dihydrouridine(20) synthase (NAD(+))
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:12003496]
EXISTENCE:direct assay:GO:0017150 tRNA dihydrouridine synthase activity [PMID:12003496]
EXISTENCE:genetic interaction:GO:0008033 tRNA processing [PMID:12559567]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:14970222]
EXISTENCE:mutant phenotype:GO:0017150 tRNA dihydrouridine synthase activity [PMID:14970222]
Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9438137]
EXISTENCE:mutant phenotype:GO:0003989 acetyl-CoA carboxylase activity [PMID:8098706]
EXISTENCE:mutant phenotype:GO:0004075 biotin carboxylase activity [PMID:6108218]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220]
EXISTENCE:mutant phenotype:GO:0042759 long-chain fatty acid biosynthetic process [PMID:6103540]
Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availability of acetyl-CoA; rate-limiting step for de novo biosynthesis of long-chain fatty acids; translational regulation in response to nutrients and cell cycle stage depends on its upstream ORF; ACC1 has a paralog, HFA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:11713477]
EXISTENCE:direct assay:GO:0030943 mitochondrion targeting sequence binding [PMID:8858146]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:10830167|PMID:7600576]
EXISTENCE:mutant phenotype:GO:0030943 mitochondrion targeting sequence binding [PMID:8858146]
EXISTENCE:physical interaction:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:7600576]
Essential component of the TIM23 complex; involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel; TIM23 complex is short for the translocase of the inner mitochondrial membrane
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22342701]
EXISTENCE:direct assay:GO:0005746 mitochondrial respirasome [PMID:22310663|PMID:22342701]
EXISTENCE:genetic interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:22310663]
EXISTENCE:mutant phenotype:GO:0010155 regulation of proton transport [PMID:31591265]
Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10672016|PMID:26928762]
EXISTENCE:genetic interaction:GO:0008204 ergosterol metabolic process [PMID:10672016]
EXISTENCE:genetic interaction:GO:0034737 ergosterol O-acyltransferase activity [PMID:10672016]
EXISTENCE:mutant phenotype:GO:0008204 ergosterol metabolic process [PMID:10672016]
EXISTENCE:mutant phenotype:GO:0034737 ergosterol O-acyltransferase activity [PMID:10672016]
Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:17135288]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:17135288]
EXISTENCE:mutant phenotype:GO:0034982 mitochondrial protein processing [PMID:17135288|PMID:17135290]
Putative metalloprotease of the mitochondrial inner membrane; required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene
mitochondrial 54S ribosomal protein MRPL50
Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8127913]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8804308]
EXISTENCE:mutant phenotype:GO:0000785 chromatin [PMID:28249159]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:28249159]
EXISTENCE:mutant phenotype:GO:0030527 structural constituent of chromatin [PMID:28249159]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8816487|PMID:10549298]
73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18591258|PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0008266 poly(U) RNA binding [PMID:18591258]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:18591258|PMID:19046973]
EXISTENCE:genetic interaction:GO:0071030 nuclear mRNA surveillance of spliceosomal pre-mRNA splicing [PMID:18591258]
EXISTENCE:genetic interaction:GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing [PMID:18591258]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:18591258]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:18591258]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia
Sar family guanine nucleotide exchange factor SEC12
EXISTENCE:direct assay:GO:0003400 regulation of COPII vesicle coating [PMID:8377826]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8377826|PMID:8583945]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8707815|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:3047151]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8377826]
Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0042823 pyridoxal phosphate biosynthetic process [PMID:9099727]
Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
peptidylprolyl isomerase family protein CPR8
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; CPR8 has a paralog, CPR4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:35584675]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:35584675]
EXISTENCE:direct assay:GO:0140827 zinc chaperone activity [PMID:35584675]
EXISTENCE:genetic interaction:GO:0034224 cellular response to zinc ion starvation [PMID:35584675]
EXISTENCE:genetic interaction:GO:0051604 protein maturation [PMID:35584675]
EXISTENCE:mutant phenotype:GO:0034224 cellular response to zinc ion starvation [PMID:35584675]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:35584675]
GTP-dependent zinc transferase; couples GTP hydrolysis to the activation of methionine aminopeptidase Map1p, especially during zinc starvation; deletion confers reduced fitness in saline; contains GTPase sequence motifs and a CxCC metal-binding motif
dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase
EXISTENCE:mutant phenotype:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:10336995]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:12460938|PMID:10336995]
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:10336995]
Alpha-1,6-mannosyltransferase localized to the ER; responsible for addition of alpha-1,6 mannose to dolichol-linked Man7GlcNAc2; acts in the dolichol pathway for N-glycosylation; human homolog ALG12 complements yeast null mutant
mitogen-activated protein kinase kinase kinase SSK2
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12857882]
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:12181352]
EXISTENCE:direct assay:GO:0004709 MAP kinase kinase kinase activity [PMID:9482735]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12181352]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12857882]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12181352|PMID:12857882]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:12181352|PMID:12857882]
EXISTENCE:genetic interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:7624781|PMID:9742096]
EXISTENCE:genetic interaction:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:9482735]
EXISTENCE:genetic interaction:GO:0071474 cellular hyperosmotic response [PMID:12857882]
EXISTENCE:genetic interaction:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:12857882]
EXISTENCE:mutant phenotype:GO:0000161 osmosensory signaling MAPK cascade [PMID:7624781]
EXISTENCE:mutant phenotype:GO:0071474 cellular hyperosmotic response [PMID:12857882]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:12181352|PMID:12857882]
EXISTENCE:physical interaction:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:9482735]
MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
putative serine/threonine-protein kinase PPG1
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:33317697]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:33317697]
EXISTENCE:mutant phenotype:GO:0005977 glycogen metabolic process [PMID:7678255]
EXISTENCE:mutant phenotype:GO:1901525 negative regulation of mitophagy [PMID:33317697]
Serine protein phosphatase involved in formation of FAR complex; inhibits mitophagy by dephosphorylating Atg32p; required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
EXISTENCE:direct assay:GO:0031386 protein tag [PMID:11923536]
EXISTENCE:direct assay:GO:0043687 post-translational protein modification [PMID:11923536]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:11923536]
EXISTENCE:mutant phenotype:GO:0033120 positive regulation of RNA splicing [PMID:31666190]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:15620657]
Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
EXISTENCE:mutant phenotype:GO:0008153 para-aminobenzoic acid biosynthetic process [PMID:8346682]
EXISTENCE:mutant phenotype:GO:0046820 4-amino-4-deoxychorismate synthase activity [PMID:8346682]
Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15454531|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:15454531]
Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
ARS1427; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26206314]
hypothetical protein; expressed during diauxic shift and stationary phase, and negatively regulated by glucose; expression is regulated by Msn2p/Msn4p; overexpression slows down progression through meiosis and improves fermentative efficiency; YNR034W-A has a paralog, YCR075W-A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407|PMID:9210376]
EXISTENCE:mutant phenotype:GO:0000226 microtubule cytoskeleton organization [PMID:14554195]
EXISTENCE:mutant phenotype:GO:0006898 receptor-mediated endocytosis [PMID:7929582]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:14554195]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0072697 protein localization to cell cortex [PMID:10749918]
EXISTENCE:physical interaction:GO:0005516 calmodulin binding [PMID:10749918]
EXISTENCE:physical interaction:GO:0005829 cytosol [PMID:14554195]
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
putative mitochondrial 37S ribosomal protein MRPS12
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:30520011]
Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
mitochondrial 37S ribosomal protein RSM19
Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S19 ribosomal protein
putative ATP-dependent RNA helicase DBP6
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9528757]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17145778|PMID:15126390]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9528757]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9528757]
Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; human homolog DDX51 complements yeast dbp6 mutant
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15961382|PMID:14562095|PMID:26928762]
EXISTENCE:genetic interaction:GO:0005385 zinc ion transmembrane transporter activity [PMID:15961382]
EXISTENCE:genetic interaction:GO:0006829 zinc ion transport [PMID:15961382]
EXISTENCE:mutant phenotype:GO:0005385 zinc ion transmembrane transporter activity [PMID:15961382]
EXISTENCE:mutant phenotype:GO:0006829 zinc ion transport [PMID:15961382|PMID:10978274]
Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:30091672]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:30091672]
EXISTENCE:genetic interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:30091672]
EXISTENCE:mutant phenotype:GO:0097177 mitochondrial ribosome binding [PMID:30091672]
Membrane-associated mitoribosome receptor; involved along with Mba1p in the cotranslational insertion of nascent polypeptide chains into the inner mitochondrial membrane; associates with the mitochondrial large ribosomal subunit and nascent polypeptide chains; null mutant displays increased tolerance to mistranslation-induced proteotoxic stress; localizes to the inner mitochondrial membrane
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10469659|PMID:1740455|PMID:16823961]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:359053]
EXISTENCE:genetic interaction:GO:0006744 ubiquinone biosynthetic process [PMID:15548532]
EXISTENCE:genetic interaction:GO:0008412 4-hydroxybenzoate octaprenyltransferase activity [PMID:7765507]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:1740455]
EXISTENCE:mutant phenotype:GO:0008412 4-hydroxybenzoate octaprenyltransferase activity [PMID:1740455]
Para hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, can complement a yeast coq2 null mutant
EXISTENCE:direct assay:GO:0004163 diphosphomevalonate decarboxylase activity [PMID:15169949|PMID:8626466]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:8626466]
EXISTENCE:mutant phenotype:GO:0004163 diphosphomevalonate decarboxylase activity [PMID:15169949|PMID:1779710]
EXISTENCE:mutant phenotype:GO:0016126 sterol biosynthetic process [PMID:9244250]
Mevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:2072914|PMID:7957044]
EXISTENCE:mutant phenotype:GO:0000752 agglutination involved in conjugation with cellular fusion [PMID:2072914|PMID:7957044]
EXISTENCE:mutant phenotype:GO:0050839 cell adhesion molecule binding [PMID:2072914|PMID:7957044]
Anchorage subunit of a-agglutinin of a-cells; highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds; AGA1 has a paralog, FIG2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8389363]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:2833749]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:2833749]
Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
EXISTENCE:direct assay:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:15899842]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14690591|PMID:22842922]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:14690591|PMID:22842922]
EXISTENCE:direct assay:GO:0008276 protein methyltransferase activity [PMID:17008308]
EXISTENCE:direct assay:GO:0018364 peptidyl-glutamine methylation [PMID:17008308]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22956767]
EXISTENCE:mutant phenotype:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:15899842]
EXISTENCE:mutant phenotype:GO:0016435 rRNA (guanine) methyltransferase activity [PMID:22493060]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:15899842]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:22956767]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:22956767]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:22956767]
EXISTENCE:mutant phenotype:GO:0070476 rRNA (guanine-N7)-methylation [PMID:22493060]
EXISTENCE:physical interaction:GO:0035657 eRF1 methyltransferase complex [PMID:17008308]
EXISTENCE:physical interaction:GO:0043528 tRNA (m2G10) methyltransferase complex [PMID:15899842]
Protein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; N7-methylates G1575 of 18S rRNA as complex with Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress; functional homolog of human TRMT112
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:18199685]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:29895620|PMID:28069741|PMID:19966303]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18199685|PMID:14562095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19966303]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:25378585]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0004674 protein serine/threonine kinase activity [PMID:19966303]
EXISTENCE:genetic interaction:GO:0044387 negative regulation of protein kinase activity by regulation of protein phosphorylation [PMID:28069741]
EXISTENCE:genetic interaction:GO:0061092 positive regulation of phospholipid translocation [PMID:18199685]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:11337509]
EXISTENCE:mutant phenotype:GO:0004672 protein kinase activity [PMID:18199685]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:19966303]
EXISTENCE:mutant phenotype:GO:2000370 positive regulation of clathrin-dependent endocytosis [PMID:28069741]
Ser/Thr protein kinase; phosphorylates several aminophospholipid translocase family members, regulating phospholipid translocation and membrane asymmetry; phosphorylates and inhibits the protein kinase Akl1p, stimulating endocytosis; phosphorylates and inhibits upstream inhibitory kinase Ypk1p; localizes to the cytoplasm, early endosome/TGN, the plasma membrane and the shmoo tip; redundant role with KIN82 in the mating pheromone response; activity stimulated by Gin4p kinase and sphingolipid MIPC
putative aminophospholipid translocase regulatory protein
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:12842877]
EXISTENCE:physical interaction:GO:0005802 trans-Golgi network [PMID:17093059]
Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:16030256|PMID:24390141]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16030256|PMID:16087665]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:16030256|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:16030256|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0006904 vesicle docking involved in exocytosis [PMID:9207091]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:16087665]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:16087665]
EXISTENCE:mutant phenotype:GO:0006904 vesicle docking involved in exocytosis [PMID:9207091]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:16030256]
EXISTENCE:mutant phenotype:GO:0061025 membrane fusion [PMID:16030256]
Lipid-interacting protein in SNARE complex assembly machinery; acts at late step in secretion; interacts with membranes through two distinct binding sites; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; N-terminus closely associates with plasma membrane, C-terminus colocalizes with Sec4p on intracellular membranes; relocalizes from bud neck to nucleus upon DNA replication stress
saccharopine dehydrogenase (NADP+, L-glutamate-forming)
EXISTENCE:direct assay:GO:0004755 saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity [PMID:18416559]
EXISTENCE:mutant phenotype:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:6429126]
Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0000454 snoRNA guided rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U990 and small subunit (SSU) rRNA at positions U120, U211, and U302
EXISTENCE:genetic interaction:GO:0045053 protein retention in Golgi apparatus [PMID:32673164]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:12778054]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:18391941]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:32673164]
EXISTENCE:mutant phenotype:GO:0060628 regulation of ER to Golgi vesicle-mediated transport [PMID:12778054]
EXISTENCE:mutant phenotype:GO:2000156 regulation of retrograde vesicle-mediated transport, Golgi to ER [PMID:14593109]
EXISTENCE:physical interaction:GO:1990861 Ubp3-Bre5 deubiquitination complex [PMID:12778054]
Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the ER and Golgi compartments, deubiquitinating COPII and COPI vesicle coat constituents, Sec23p and Sec27p; involved along with Ubp3p in the steady-state retention of Golgi membrane proteins, such as glycosyltransferases; null is sensitive to brefeldin A
CCR4-NOT core DEDD family RNase subunit POP2
EXISTENCE:direct assay:GO:0000175 3'-5'-exoribonuclease activity [PMID:11410650]
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11410650|PMID:11889048]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18611963]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0030015 CCR4-NOT core complex [PMID:10490603]
EXISTENCE:mutant phenotype:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11410650]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
Subunit of Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation; exonuclease of the DEDD superfamily
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:26928762]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:11707418]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11707418]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:22735702|PMID:17443350|PMID:23212245]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:24240281]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11583615|PMID:11707418]
EXISTENCE:mutant phenotype:GO:2000200 regulation of ribosomal subunit export from nucleus [PMID:24240281]
Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:12810910]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:12810910]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:12810910]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:12810910|PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2258 and U2260; encoded within the intron of NOG2/YNR053C
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:16772403]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:16772403]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15964808|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15964808]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15964808]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15964808]
EXISTENCE:mutant phenotype:GO:0034462 small-subunit processome assembly [PMID:15964808]
Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
EXISTENCE:mutant phenotype:GO:0006812 cation transport [PMID:8955402]
EXISTENCE:mutant phenotype:GO:0015665 alcohol transmembrane transporter activity [PMID:2405251]
EXISTENCE:mutant phenotype:GO:0015850 organic hydroxy compound transport [PMID:2405251]
EXISTENCE:mutant phenotype:GO:0022890 inorganic cation transmembrane transporter activity [PMID:8955402]
Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake
EXISTENCE:direct assay:GO:0022857 transmembrane transporter activity [PMID:10333520]
EXISTENCE:direct assay:GO:0051180 vitamin transport [PMID:10333520]
EXISTENCE:mutant phenotype:GO:0009102 biotin biosynthetic process [PMID:10333520]
EXISTENCE:mutant phenotype:GO:0051180 vitamin transport [PMID:10333520]
Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
EXISTENCE:direct assay:GO:0004141 dethiobiotin synthase activity [PMID:10333520]
EXISTENCE:direct assay:GO:0009102 biotin biosynthetic process [PMID:10333520]
Dethiobiotin synthetase; catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria; expression appears to be repressed at low iron levels
adenosylmethionine-8-amino-7-oxononanoate transaminase
EXISTENCE:direct assay:GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity [PMID:10333520]
7,8-diamino-pelargonic acid aminotransferase (DAPA); catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria
EXISTENCE:curator inference:GO:0006493 protein O-linked glycosylation [PMID:10521541]
Putative alpha-1,3-mannosyltransferase; not required for protein O-glycosylation; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:genetic interaction:GO:0000293 ferric-chelate reductase activity [PMID:11120744]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:11120744]
EXISTENCE:mutant phenotype:GO:0015891 siderophore transport [PMID:11120744]
Ferric reductase; reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
hypothetical protein; relocalizes from vacuole to cytoplasm upon DNA replication stress
Putative pulcherrimin transporter; involved in uptake of iron-transporting siderophore, pulcherrimin
Putative zinc-cluster transcription factor; involved in utilization of iron-transporting siderophore pulcherrimin
EXISTENCE:direct assay:GO:0004301 epoxide hydrolase activity [PMID:15769598]
EXISTENCE:direct assay:GO:0097176 epoxide metabolic process [PMID:15769598]
Epoxide hydrolase; member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
hypothetical protein; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11935221]
Putative membrane-localized hypothetical protein
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11935221]
EXISTENCE:direct assay:GO:0030428 cell septum [PMID:12455695]
EXISTENCE:genetic interaction:GO:0042973 glucan endo-1,3-beta-D-glucosidase activity [PMID:12455695]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:16328626]
Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother
hypothetical protein; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
hypothetical protein; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
ATP-binding cassette multidrug transporter PDR18
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21831043]
EXISTENCE:mutant phenotype:GO:0008559 ABC-type xenobiotic transporter activity [PMID:21831043]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:21831043]
EXISTENCE:mutant phenotype:GO:0055092 sterol homeostasis [PMID:21831043]
EXISTENCE:mutant phenotype:GO:0071466 cellular response to xenobiotic stimulus [PMID:21831043]
Transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:genetic interaction:GO:0015795 sorbitol transmembrane transport [PMID:26996892]
EXISTENCE:genetic interaction:GO:0015797 mannitol transmembrane transport [PMID:26996892]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Putative transmembrane polyol transporter; supports growth on and uptake of mannitol and sorbitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; induced by raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose; HXT17 has a paralog, HXT13, that arose from a segmental duplication
EXISTENCE:mutant phenotype:GO:0046029 mannitol dehydrogenase activity [PMID:26996892]
Mannitol dehydrogenase; MAN2 has a paralog, DSF1, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15381687]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15381687]
EXISTENCE:mutant phenotype:GO:0000304 response to singlet oxygen [PMID:11409174]
EXISTENCE:mutant phenotype:GO:0043065 positive regulation of apoptotic process [PMID:15381687]
Mitochondrial cell death effector; translocates to the nucleus in response to apoptotic stimuli; triggers apoptosis in cells lacking HXK2; homolog of mammalian Apoptosis-Inducing Factor, putative reductase
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11914276]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276|PMID:14562095]
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme; identical to Pau18p
TEL14R; Telomeric region on the right arm of Chromosome XIV; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats
hypothetical protein, abundance changes with carbon source