Saccharomyces cerevisiae S288C chromosome XIII, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006946.\n\nOn Apr 26, 2011 this sequence version replaced NC_001145.2.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##
TEL13L; Telomeric region on the left arm of Chromosome XIII; composed of an X element core sequence, X element combinatorial repeats, a short region of telomeric repeats, a short Y' element, and a short terminal region of telomeric repeats
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
Putative Y' element ATP-dependent helicase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YML133C contains an intron
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14718542|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:14718542]
EXISTENCE:mutant phenotype:GO:0006883 cellular sodium ion homeostasis [PMID:14718542]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
hypothetical protein; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9659914|PMID:26928762]
EXISTENCE:direct assay:GO:0016972 thiol oxidase activity [PMID:16407158]
EXISTENCE:mutant phenotype:GO:0034975 protein folding in endoplasmic reticulum [PMID:9659913]
Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining ER redox balance; feedback regulated via reduction and oxidation of regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds; ero1-1 mutation complemented by human ERO1L
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:7797555]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15306853]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:15306853]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:21068384|PMID:7797555]
EXISTENCE:physical interaction:GO:0070130 negative regulation of mitochondrial translation [PMID:15306853]
Mitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511544]
hypothetical protein; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:11931764]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:11931764|PMID:12183366]
EXISTENCE:direct assay:GO:0070301 cellular response to hydrogen peroxide [PMID:11931764]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11931764]
EXISTENCE:mutant phenotype:GO:0009303 rRNA transcription [PMID:11931764]
Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0004421 hydroxymethylglutaryl-CoA synthase activity [PMID:6148937|PMID:12702274]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:6148937|PMID:12702274]
3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19239415]
EXISTENCE:mutant phenotype:GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H [PMID:19239415]
EXISTENCE:mutant phenotype:GO:0015031 protein transport [PMID:16943325]
Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0000070 mitotic sister chromatid segregation [PMID:3540600]
EXISTENCE:curator inference:GO:0005828 kinetochore microtubule [PMID:3540600]
EXISTENCE:curator inference:GO:0005880 nuclear microtubule [PMID:3540600]
EXISTENCE:curator inference:GO:0005881 cytoplasmic microtubule [PMID:3540600]
EXISTENCE:curator inference:GO:0030473 nuclear migration along microtubule [PMID:3540600]
EXISTENCE:curator inference:GO:0045143 homologous chromosome segregation [PMID:3540600]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:24390141]
EXISTENCE:genetic interaction:GO:0045298 tubulin complex [PMID:3540600]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:10793159]
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
Antisense to PHO84 and beginning of YML122C; represses transcription of PHO84 through histone deacetylation
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:7851439]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2038328]
EXISTENCE:direct assay:GO:0006817 phosphate ion transport [PMID:7851439]
EXISTENCE:direct assay:GO:0097079 selenite:proton symporter activity [PMID:20688911]
EXISTENCE:mutant phenotype:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:2038328]
EXISTENCE:mutant phenotype:GO:0005384 manganese ion transmembrane transporter activity [PMID:12923174]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:17804816]
EXISTENCE:mutant phenotype:GO:0006817 phosphate ion transport [PMID:2038328]
EXISTENCE:mutant phenotype:GO:0006828 manganese ion transport [PMID:12923174]
EXISTENCE:mutant phenotype:GO:0097079 selenite:proton symporter activity [PMID:20688911]
EXISTENCE:mutant phenotype:GO:0097080 plasma membrane selenite transport [PMID:20688911]
High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
hypothetical protein; conserved among S. cerevisiae strains; YML122C is not an essential gene
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15885099|PMID:19748353|PMID:26928762]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:22726655|PMID:23716719]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10388807]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10388807]
EXISTENCE:direct assay:GO:0031902 late endosome membrane [PMID:16732272]
EXISTENCE:direct assay:GO:0071986 Ragulator complex [PMID:16732272]
EXISTENCE:direct assay:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:25046117]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:15937128]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:15937128]
EXISTENCE:mutant phenotype:GO:0006817 phosphate ion transport [PMID:1620108]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:25046117]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:18258182]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:35158208]
EXISTENCE:mutant phenotype:GO:1903778 protein localization to vacuolar membrane [PMID:25046117]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353|PMID:26387955]
EXISTENCE:physical interaction:GO:0006360 transcription by RNA polymerase I [PMID:15937128]
EXISTENCE:physical interaction:GO:0006383 transcription by RNA polymerase III [PMID:15937128]
EXISTENCE:physical interaction:GO:1990131 Gtr1-Gtr2 GTPase complex [PMID:10388807|PMID:16143306]
Subunit of TORC1-stimulating GTPase and EGO/GSE complex; subunit of Gtr1-Gtr2 GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p; involved in phosphate transport, telomeric chromatin silencing and oxidative stress response; activated by Iml1p (GAP) subunit of SEACIT complex; targeted to vacuole via AP-3 pathway; similar to human RagA and RagB
NADH-ubiquinone reductase (H(+)-translocating) NDI1
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:1900238]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:3138118]
EXISTENCE:direct assay:GO:0006120 mitochondrial electron transport, NADH to ubiquinone [PMID:1900238]
EXISTENCE:direct assay:GO:0008137 NADH dehydrogenase (ubiquinone) activity [PMID:3138118]
EXISTENCE:mutant phenotype:GO:0005759 mitochondrial matrix [PMID:1900238]
EXISTENCE:mutant phenotype:GO:0043065 positive regulation of apoptotic process [PMID:16436509]
NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain but does not pump protons, in contrast to higher eukaryotic multisubunit respiratory complex I; upon apoptotic stress, is activated in mitochondria by N-terminal cleavage, then translocates to cytoplasm to induce apoptosis; homolog of human AIFM2; yeast NDI1 complements several phenotypes of human cell line with mutated MT-ND4, implicated in Leber hereditary optic neuropathy
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
hypothetical protein; YML119W is not an essential gene; potential Cdc28p substrate
ARS1303; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004535 poly(A)-specific ribonuclease activity [PMID:21965533]
EXISTENCE:direct assay:GO:0043633 polyadenylation-dependent RNA catabolic process [PMID:21965533]
3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
EXISTENCE:direct assay:GO:0005773 vacuole [PMID:19414602]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19414602]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:2123132]
EXISTENCE:mutant phenotype:GO:0046713 borate transport [PMID:19414602]
EXISTENCE:mutant phenotype:GO:0080139 borate efflux transmembrane transporter activity [PMID:19414602]
Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
ARS1304; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:12235155]
EXISTENCE:direct assay:GO:0000136 mannan polymerase complex [PMID:9430634]
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:9430634]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:12235155]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9430634]
Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:15448131]
EXISTENCE:curator inference:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10788514]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation
EXISTENCE:direct assay:GO:0003680 minor groove of adenine-thymine-rich DNA binding [PMID:2670564]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8248247]
DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15520468]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15520468]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:11118453]
EXISTENCE:direct assay:GO:0032786 positive regulation of DNA-templated transcription, elongation [PMID:9110987]
EXISTENCE:direct assay:GO:0070692 CTDK-1 complex [PMID:7565723]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:11118453]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15520468]
EXISTENCE:physical interaction:GO:0045903 positive regulation of translational fidelity [PMID:17545469]
Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0034450 ubiquitin-ubiquitin ligase activity [PMID:11352928]
EXISTENCE:mutant phenotype:GO:0000151 ubiquitin ligase complex [PMID:11352928]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:9931424]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:9931424]
Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex; ubiquitin-binding adaptor involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication
2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:9083048]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:9083048]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9083049]
EXISTENCE:mutant phenotype:GO:0043334 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity [PMID:9083049]
2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; respiratory defect of the null mutant is partially complemented by human COQ5
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21536748]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:20980617]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:21536748]
EXISTENCE:genetic interaction:GO:0010971 positive regulation of G2/M transition of mitotic cell cycle [PMID:20980617]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:8816439]
EXISTENCE:genetic interaction:GO:0032880 regulation of protein localization [PMID:18762578]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:10662670]
EXISTENCE:mutant phenotype:GO:0010971 positive regulation of G2/M transition of mitotic cell cycle [PMID:21536748]
Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
hypothetical protein; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:16162818]
EXISTENCE:mutant phenotype:GO:0051237 maintenance of RNA location [PMID:16162818]
Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes
EXISTENCE:direct assay:GO:0004588 orotate phosphoribosyltransferase activity [PMID:9882434]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0046132 pyrimidine ribonucleoside biosynthetic process [PMID:9882434]
EXISTENCE:mutant phenotype:GO:0004588 orotate phosphoribosyltransferase activity [PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:4550660]
EXISTENCE:mutant phenotype:GO:0046132 pyrimidine ribonucleoside biosynthetic process [PMID:4550660]
Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
RNA-binding signal recognition particle subunit SEC65
EXISTENCE:direct assay:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:1313947|PMID:7925282]
EXISTENCE:direct assay:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:10921896]
EXISTENCE:direct assay:GO:0008312 7S RNA binding [PMID:14681587]
EXISTENCE:mutant phenotype:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:1313948]
EXISTENCE:mutant phenotype:GO:0006617 SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition [PMID:1313947]
Subunit of the signal recognition particle (SRP); involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:26283797]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:2202739|PMID:9245780]
EXISTENCE:mutant phenotype:GO:1990854 vacuole-ER tethering [PMID:26283797]
PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs; anchored in the ER membrane at nucleus-vacuole junctions and binds phosphatidylinositol 3-phosphate (PtdIns-3-P) in the vacuolar membrane via its Phox homology (PX) domain; expressed predominantly in late G1 to early S phase of the cell cycle; mutation affects nuclear and mitochondrial transmission to daughter buds; similar to 4 human genes, one of which (SNX14) is associated with neurological disease
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0044611 nuclear pore inner ring [PMID:18046406]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:16361228]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:8682855]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:16361228|PMID:8682855]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:8682855]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809]
Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15923376]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); predicted to guide pseudouridylation of small subunit (SSU) rRNA at position U1181
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:11782447]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0006335 DNA replication-dependent chromatin assembly [PMID:9030687]
EXISTENCE:direct assay:GO:0033186 CAF-1 complex [PMID:9030687]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:16503640]
EXISTENCE:mutant phenotype:GO:0006335 DNA replication-dependent chromatin assembly [PMID:9030687]
Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
hypothetical protein; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
trehalose 6-phosphate synthase/phosphatase complex subunit
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:8404905]
EXISTENCE:genetic interaction:GO:0004805 trehalose-phosphatase activity [PMID:9837904]
EXISTENCE:mutant phenotype:GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity [PMID:9837904]
EXISTENCE:mutant phenotype:GO:0004805 trehalose-phosphatase activity [PMID:9837904]
EXISTENCE:mutant phenotype:GO:0005992 trehalose biosynthetic process [PMID:9837904]
EXISTENCE:physical interaction:GO:0005946 alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [PMID:9837904]
Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:10688655]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:3311884]
EXISTENCE:mutant phenotype:GO:0000821 regulation of arginine metabolic process [PMID:10688655]
Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity []
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12840001]
TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10329739|PMID:23612966]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:31754649]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8628304|PMID:23612966|PMID:26928762]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12628920]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:23612966]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:23612966]
EXISTENCE:genetic interaction:GO:0036010 protein localization to endosome [PMID:23264632]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:1493335]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8628304]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:22748138|PMID:23612966]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:23612966]
Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain
Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:8253764]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:1741062]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9852079]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:7559571]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:11514439]
EXISTENCE:mutant phenotype:GO:0000735 removal of nonhomologous ends [PMID:7891718]
EXISTENCE:mutant phenotype:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:7891718]
EXISTENCE:mutant phenotype:GO:0006277 DNA amplification [PMID:23271978]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:2188090]
EXISTENCE:physical interaction:GO:0000110 nucleotide-excision repair factor 1 complex [PMID:8621533]
Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:24068951]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9463374]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:9463374]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:physical interaction:GO:0016272 prefoldin complex [PMID:9463374]
Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
proteasome core particle subunit alpha 2
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:9087403]
EXISTENCE:curator inference:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:9087403]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019773 proteasome core complex, alpha-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:direct assay:GO:0001682 tRNA 5'-leader removal [PMID:8366116]
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:8366116]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16024791]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:7544434]
EXISTENCE:direct assay:GO:0030677 ribonuclease P complex [PMID:8366116]
EXISTENCE:direct assay:GO:0030678 mitochondrial ribonuclease P complex [PMID:3537697]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16024791]
EXISTENCE:genetic interaction:GO:0006397 mRNA processing [PMID:17267405]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:11404323]
EXISTENCE:mutant phenotype:GO:0006396 RNA processing [PMID:11522833]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11404323]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16024791]
EXISTENCE:mutant phenotype:GO:0097745 mitochondrial tRNA 5'-end processing [PMID:30759361]
EXISTENCE:physical interaction:GO:0006397 mRNA processing [PMID:17267405]
Protein subunit of mitochondrial RNase P; has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
tDNA relic, ancient tDNA for tRNA-Ile; weakly transcribed by RNA polymerase III; located upstream of UFO1; associated with components of the Pol III machinery: Tfc4p, Brf1p, Bdp1, and Rpc34p
SCF ubiquitin ligase complex subunit UFO1
EXISTENCE:curator inference:GO:0004842 ubiquitin-protein transferase activity [PMID:14506225|PMID:14747994]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14506225]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14506225|PMID:14562095]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:11283612|PMID:14747994]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:10963670]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:10963670]
EXISTENCE:mutant phenotype:GO:0071406 cellular response to methylmercury [PMID:17141224]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14506225|PMID:14747994]
EXISTENCE:physical interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:14506225]
F-box receptor protein; subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitination by SCF and subsequent degradation
ARS1305; Autonomously Replicating Sequence
hypothetical protein, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003885 D-arabinono-1,4-lactone oxidase activity [PMID:10094636]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10094636|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:16407407]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10094636]
EXISTENCE:mutant phenotype:GO:0070485 dehydro-D-arabinono-1,4-lactone biosynthetic process [PMID:10094636]
D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress; null mutant shows improved incorporation efficiency of noncanonical amino acids in place of amber stop codon
EXISTENCE:curator inference:GO:0005827 polar microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0005828 kinetochore microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0005880 nuclear microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0005881 cytoplasmic microtubule [PMID:9488492]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:direct assay:GO:0045298 tubulin complex [PMID:9488492]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:16582437]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:16582437]
EXISTENCE:mutant phenotype:GO:0045143 homologous chromosome segregation [PMID:16582437]
EXISTENCE:physical interaction:GO:0045298 tubulin complex [PMID:9488492]
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
hypothetical protein; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; not an essential gene; YML082W has a paralog, STR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005753 mitochondrial proton-transporting ATP synthase complex [PMID:20219971]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:9867807]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:9867807|PMID:9867878]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:20219971]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:9867807]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:20219971]
Subunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:16785442|PMID:19111667|PMID:19158363]
EXISTENCE:mutant phenotype:GO:0019413 acetate biosynthetic process [PMID:23240040]
Transcription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:12003496]
EXISTENCE:direct assay:GO:0017150 tRNA dihydrouridine synthase activity [PMID:12003496]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:14970222]
EXISTENCE:mutant phenotype:GO:0017150 tRNA dihydrouridine synthase activity [PMID:14970222]
Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
Protein required for production of 4-hydroxy-5-methylfuran-3(2H)-one (MHF); cupin superfamily protein; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to nucleus and cytoplasm
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:7603990]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7603990|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:7603990]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17881727]
Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17110339]
EXISTENCE:direct assay:GO:1990070 TRAPPI protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990072 TRAPPIII protein complex [PMID:20375281]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:9611195]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9611195]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17287728]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11239471|PMID:9611195]
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homology TRAPPC1 complements yeast null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12588995]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:12588995]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12588995]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:12588995]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12588995]
Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
hydroxymethylglutaryl-CoA reductase (NADPH) HMG1
EXISTENCE:curator inference:GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway [PMID:3526336]
EXISTENCE:direct assay:GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity [PMID:3526336]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:8744950]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8744950]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8744950]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:3526336]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922|PMID:26928762]
EXISTENCE:genetic interaction:GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity [PMID:2828155]
EXISTENCE:mutant phenotype:GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity [PMID:2828155]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:9292983]
HMG-CoA reductase; catalyzes conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to nuclear envelope; overproduction induces formation of karmellae; forms foci at nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg1 mutant
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:7525596|PMID:7925954|PMID:8051210]
EXISTENCE:direct assay:GO:0005527 macrolide binding [PMID:7925954]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26359986]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:7525596|PMID:8051210]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:24297734]
EXISTENCE:direct assay:GO:0051598 meiotic recombination checkpoint signaling [PMID:16179256]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:16179256]
Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:3282992]
Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:33933446]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:13679573]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:15049706]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:23237950|PMID:22250200]
EXISTENCE:direct assay:GO:0120010 intermembrane phospholipid transfer [PMID:33933446]
EXISTENCE:genetic interaction:GO:0055091 phospholipid homeostasis [PMID:35440494]
EXISTENCE:genetic interaction:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:23237950]
EXISTENCE:genetic interaction:GO:0090158 endoplasmic reticulum membrane organization [PMID:23237950]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:33205016]
EXISTENCE:mutant phenotype:GO:0120010 intermembrane phospholipid transfer [PMID:33933446]
Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane; regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; involved in intermembrane phospholipid transfer; also localizes to the mitochondria in a phosphorylated state and is enriched in bud membranes; mRNA is targeted to the bud tip
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0017119 Golgi transport complex [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20065092]
Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:genetic interaction:GO:0004371 glycerone kinase activity [PMID:12401799]
EXISTENCE:genetic interaction:GO:0061610 glycerol to glycerone phosphate metabolic process [PMID:22979944]
EXISTENCE:mutant phenotype:GO:0004371 glycerone kinase activity [PMID:12401799]
EXISTENCE:mutant phenotype:GO:0097237 cellular response to toxic substance [PMID:12401799]
Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10413469]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10413469]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:15082784|PMID:19683499]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:11432837]
EXISTENCE:direct assay:GO:0035101 FACT complex [PMID:9705338]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:18089575]
EXISTENCE:direct assay:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15987999]
EXISTENCE:genetic interaction:GO:0006261 DNA-templated DNA replication [PMID:10924459]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:10924459]
EXISTENCE:physical interaction:GO:0006261 DNA-templated DNA replication [PMID:9199353]
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
ARS1307; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0006449 regulation of translational termination [PMID:11570975]
Protein that modulates the efficiency of translation termination; interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:11157978]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11157978]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11157978]
Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:17298976|PMID:18756268|PMID:24390141]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17298976]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0070583 spore membrane bending pathway [PMID:18756268]
Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11168584]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:17825064]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11168584]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051|PMID:16581798]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:31263106]
EXISTENCE:direct assay:GO:0043007 maintenance of rDNA [PMID:31263106]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16581798]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:9038340]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:9335335]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16716188]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:9038340]
Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; ORC1 has a paralog, SIR3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:12048186|PMID:25658911]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:19154724]
EXISTENCE:direct assay:GO:0023056 positive regulation of signaling [PMID:32074005]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:25658911]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:21321099]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:25658911]
EXISTENCE:mutant phenotype:GO:0040001 establishment of mitotic spindle localization [PMID:21937712]
EXISTENCE:mutant phenotype:GO:1902542 regulation of protein localization to mitotic spindle pole body [PMID:25658911]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:10219244]
GTPase involved in initiation of Mitotic Exit Network (MEN); GTP-binding protein of the Ras superfamily; accumulates at daughter spindle pole body and activates MEN kinase cascade; controls actomyosin and septin dynamics during cytokinesis
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Ribosomal protein of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:24084588]
EXISTENCE:direct assay:GO:0003676 nucleic acid binding [PMID:12093753]
EXISTENCE:mutant phenotype:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0000446 nucleoplasmic THO complex [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12093753]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:27251550]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:16908536]
Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:8253734]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:23596008]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:26411679]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1849081|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:31744872]
EXISTENCE:direct assay:GO:0010521 telomerase inhibitor activity [PMID:16121131]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:30442759]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:20655619]
EXISTENCE:direct assay:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:16121131]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:30068549]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:26981397]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:23657261]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:genetic interaction:GO:0071932 replication fork reversal [PMID:26411679]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:15907372]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:10926538]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:24025772]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:1849081]
EXISTENCE:mutant phenotype:GO:0010521 telomerase inhibitor activity [PMID:16121131]
EXISTENCE:mutant phenotype:GO:0019985 translesion synthesis [PMID:30107417]
EXISTENCE:mutant phenotype:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:16121131]
EXISTENCE:mutant phenotype:GO:0044806 G-quadruplex DNA unwinding [PMID:23657261]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:8287473]
EXISTENCE:mutant phenotype:GO:0098781 ncRNA transcription [PMID:31391265]
DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; involved in crossover recombination; works with Hrq1p to maintain telomere length homeostasis; translation from different start sites produces mitochondrial (DNA repair and recombination) and nuclear (catalytic inhibitor of telomerase) forms; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11239005]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11239005]
EXISTENCE:direct assay:GO:0006285 base-excision repair, AP site formation [PMID:8643552]
EXISTENCE:direct assay:GO:0008534 oxidized purine nucleobase lesion DNA N-glycosylase activity [PMID:8643552]
EXISTENCE:genetic interaction:GO:0070987 error-free translesion synthesis [PMID:19264809]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:11239005]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:19942858]
Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
EXISTENCE:direct assay:GO:0004622 lysophospholipase activity [PMID:15044461]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15044461|PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0034638 phosphatidylcholine catabolic process [PMID:15044461]
EXISTENCE:mutant phenotype:GO:0071071 regulation of phospholipid biosynthetic process [PMID:19841481]
Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
EXISTENCE:genetic interaction:GO:1905117 regulation of ribonucleoside-diphosphate reductase activity [PMID:25378334]
EXISTENCE:genetic interaction:GO:1990846 ribonucleoside-diphosphate reductase inhibitor activity [PMID:25378334]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:10490641]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:10490641]
Ribonucleotide reductase inhibitor; intrinsically disordered protein that binds to and inhibits Rnr2p; involved in the Mec1p-mediated checkpoint pathway; transcription is induced by genotoxic stress and by activation of the Rad53p pathway; protein abundance increases in response to DNA replication stress
ARS1307.5; Autonomously replicating sequence
ribonucleotide reductase inhibiting protein SML1
EXISTENCE:direct assay:GO:1905117 regulation of ribonucleoside-diphosphate reductase activity [PMID:10593972]
EXISTENCE:direct assay:GO:1990846 ribonucleoside-diphosphate reductase inhibitor activity [PMID:10593972|PMID:27155231]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:9774971]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:9774971]
Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004723 calcium-dependent protein serine/threonine phosphatase activity [PMID:1321337]
EXISTENCE:direct assay:GO:0005955 calcineurin complex [PMID:1321337]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:1651503]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:8798496]
Calcineurin A; one isoform (the other is Cna1p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CMP2 has a paralog, CNA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position A974
EXISTENCE:direct assay:GO:0005787 signal peptidase complex [PMID:1846444|PMID:8910564]
EXISTENCE:genetic interaction:GO:0045047 protein targeting to ER [PMID:8910564]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:10921929]
EXISTENCE:mutant phenotype:GO:0008233 peptidase activity [PMID:10921929]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:8910564]
EXISTENCE:mutant phenotype:GO:0120236 negative regulation of post-translational protein targeting to membrane, translocation [PMID:32513868]
EXISTENCE:physical interaction:GO:0045047 protein targeting to ER [PMID:8910564]
Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; inhibits SRP-independent translocation into the ER with STE24; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p
EXISTENCE:direct assay:GO:0004460 L-lactate dehydrogenase (cytochrome) activity [PMID:3004948]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:3004948|PMID:6290489]
EXISTENCE:mutant phenotype:GO:0006089 lactate metabolic process [PMID:8492799]
Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:17507646]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11784867|PMID:16496001]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:15536122|PMID:16496001]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16496001]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11784867]
EXISTENCE:mutant phenotype:GO:0097446 protein localization to eisosome filament [PMID:25811606]
Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches)
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10866670|PMID:18952899]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10866670|PMID:18952899]
EXISTENCE:direct assay:GO:0019210 kinase inhibitor activity [PMID:18957435]
EXISTENCE:direct assay:GO:0033673 negative regulation of kinase activity [PMID:18957435]
EXISTENCE:direct assay:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:9811836]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9811836]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:6092916]
EXISTENCE:mutant phenotype:GO:0006012 galactose metabolic process [PMID:6092916]
EXISTENCE:physical interaction:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:9811836]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9811836]
Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:34461091]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:30879301|PMID:34461091]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:34461091]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:34461091]
2Fe-2S mitochondrial protein involved in redox quality control; may maintain protein redox status by targeting oxidation sensitive cysteine residues; bishistidinyl coordinated, non-Rieske [2Fe-2S] cluster containing thiredoxin-like ferredoxin; forms a functional complex with Osm1p and Erv1p in the IMS; required for assembly of numerous mitochondrial import complexes; essential for anaerobiosis; localizes to the mitochondrial matrix and intermembrane space (IMS); homologous to APD1
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:10369685]
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:15565172|PMID:16314500]
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:10369685]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9819400]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:16314500]
Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10377429]
EXISTENCE:genetic interaction:GO:0034394 protein localization to cell surface [PMID:10377429]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15623581]
EXISTENCE:mutant phenotype:GO:0034394 protein localization to cell surface [PMID:10377429]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:15623581]
Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
pheromone-regulated K(+) transporter PRM6
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23204190]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:23204190]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:23204190]
EXISTENCE:genetic interaction:GO:0015079 potassium ion transmembrane transporter activity [PMID:23204190]
EXISTENCE:genetic interaction:GO:0071805 potassium ion transmembrane transport [PMID:23204190]
Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; member of a fungal-specific gene family; PRM6 has a paralog, KCH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:8196608|PMID:9630245]
EXISTENCE:genetic interaction:GO:0000243 commitment complex [PMID:8196608]
EXISTENCE:genetic interaction:GO:0000395 mRNA 5'-splice site recognition [PMID:8196608]
EXISTENCE:genetic interaction:GO:0030627 pre-mRNA 5'-splice site binding [PMID:8196608]
U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
ARS1308; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
YMLWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
EXISTENCE:curator inference:GO:0001164 RNA polymerase I core promoter sequence-specific DNA binding []
EXISTENCE:direct assay:GO:0001164 RNA polymerase I core promoter sequence-specific DNA binding [PMID:18451108]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:23613772]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:8702872]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8887672]
EXISTENCE:direct assay:GO:0070860 RNA polymerase I core factor complex [PMID:8702872|PMID:8887672]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8702872]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8702872]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:8702872]
Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:18427809|PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:18427809|PMID:22375831]
EXISTENCE:mutant phenotype:GO:0004092 carnitine O-acetyltransferase activity [PMID:8420957]
EXISTENCE:mutant phenotype:GO:0009437 carnitine metabolic process [PMID:11329169]
Carnitine acetyl-CoA transferase; present in both mitochondria and peroxisomes; transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:14690608]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:16299513]
Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons
YMLWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0015786 UDP-glucose transmembrane transport [PMID:18693752]
Putative nucleotide sugar transporter; has similarity to Vrg4p
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
hypothetical protein; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:33277478]
EXISTENCE:direct assay:GO:0000408 EKC/KEOPS complex [PMID:16564010|PMID:16874308]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:mutant phenotype:GO:0000049 tRNA binding [PMID:33277478]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:25822194]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:23390378]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16874308]
Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p; ortholog of human TPRKB
EXISTENCE:direct assay:GO:0003876 AMP deaminase activity [PMID:2690949]
EXISTENCE:genetic interaction:GO:0006163 purine nucleotide metabolic process [PMID:15869715|PMID:18433446|PMID:19635936]
EXISTENCE:genetic interaction:GO:0006178 guanine salvage [PMID:19635936]
EXISTENCE:mutant phenotype:GO:0003876 AMP deaminase activity [PMID:2690949]
EXISTENCE:mutant phenotype:GO:0006163 purine nucleotide metabolic process [PMID:19635936|PMID:20087341]
EXISTENCE:mutant phenotype:GO:0006178 guanine salvage [PMID:19635936]
AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:18762579]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:16929305]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:genetic interaction:GO:0000070 mitotic sister chromatid segregation [PMID:11754482]
EXISTENCE:genetic interaction:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:11754482]
EXISTENCE:mutant phenotype:GO:0043007 maintenance of rDNA [PMID:18997772]
Chromosome linkage inner nuclear membrane protein; CLIP, involved in perinuclear chromosome tethering and rDNA repeat stability; enriched at telomeres and subtelomeric regions; associates with and regulates subtelomeric genes, interacting with TREX (transcription export) factors; produces two splice variants with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant is aneuploidy tolerant; SRC1 has a paralog, HEH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000150 DNA strand exchange activity [PMID:15205482]
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:12766777]
EXISTENCE:direct assay:GO:0000730 DNA recombinase assembly [PMID:11459983]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11459964|PMID:22842922]
EXISTENCE:direct assay:GO:1904877 positive regulation of DNA ligase activity [PMID:32083180]
EXISTENCE:direct assay:GO:1990814 DNA/DNA annealing activity [PMID:27362509]
EXISTENCE:genetic interaction:GO:0000709 meiotic joint molecule formation [PMID:18313389]
EXISTENCE:genetic interaction:GO:0045002 double-strand break repair via single-strand annealing [PMID:11606529]
EXISTENCE:mutant phenotype:GO:0000709 meiotic joint molecule formation [PMID:18313389]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:11238918|PMID:25822194]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:17096599|PMID:24190138]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:17096599]
EXISTENCE:mutant phenotype:GO:0006301 postreplication repair [PMID:7038396]
EXISTENCE:mutant phenotype:GO:0043504 mitochondrial DNA repair [PMID:26540255]
EXISTENCE:mutant phenotype:GO:1904877 positive regulation of DNA ligase activity [PMID:32083180]
Protein involved in homologous recombination; attenuates resection of DNA double-strand break ends and stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination; phosphorylation by Cdc28p in G2/M phase promotes Rad52p ring interaction that leads to superstructure formation
EXISTENCE:curator inference:GO:0006913 nucleocytoplasmic transport [PMID:9864355]
EXISTENCE:curator inference:GO:0017056 structural constituent of nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:20498018]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:9864355]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:9864355]
EXISTENCE:direct assay:GO:0070762 nuclear pore transmembrane ring [PMID:10684247|PMID:19414609]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:15075274|PMID:16682526]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:16682526]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:15075274|PMID:16682526]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:15075274|PMID:8349727]
EXISTENCE:physical interaction:GO:0070762 nuclear pore transmembrane ring [PMID:19414609]
Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22405070|PMID:22310663|PMID:22342701]
EXISTENCE:direct assay:GO:0005746 mitochondrial respirasome [PMID:22405070|PMID:22310663|PMID:22342701]
EXISTENCE:genetic interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:22310663]
EXISTENCE:mutant phenotype:GO:0010155 regulation of proton transport [PMID:31591265]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:29746825]
EXISTENCE:mutant phenotype:GO:0097250 mitochondrial respirasome assembly [PMID:22405070|PMID:22342701]
Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:20005842]
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:31713515]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16873066]
EXISTENCE:mutant phenotype:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16873066]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:11425851]
EXISTENCE:physical interaction:GO:0005789 endoplasmic reticulum membrane [PMID:16873066]
EXISTENCE:physical interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16873066]
Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10681558|PMID:8344960|PMID:22842922]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18271751]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:18271751]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:15163410]
EXISTENCE:direct assay:GO:0008379 thioredoxin peroxidase activity [PMID:7961686|PMID:9799566]
EXISTENCE:direct assay:GO:0034599 cellular response to oxidative stress [PMID:8344960]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:18271751]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:8344960]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:15163410]
EXISTENCE:direct assay:GO:0051920 peroxiredoxin activity [PMID:17210445]
EXISTENCE:genetic interaction:GO:0000077 DNA damage checkpoint signaling [PMID:19851444]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:15051715]
EXISTENCE:mutant phenotype:GO:0006111 regulation of gluconeogenesis [PMID:27634403]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:16251355]
EXISTENCE:mutant phenotype:GO:0008379 thioredoxin peroxidase activity [PMID:7961686|PMID:9799566]
EXISTENCE:mutant phenotype:GO:0019207 kinase regulator activity [PMID:27634403]
EXISTENCE:mutant phenotype:GO:0033194 response to hydroperoxide [PMID:15210711]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:18271751|PMID:8344960]
EXISTENCE:mutant phenotype:GO:0042262 DNA protection [PMID:19543365]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:9799566|PMID:8344960]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:16251355]
EXISTENCE:mutant phenotype:GO:0061077 chaperone-mediated protein folding [PMID:24022485]
Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form a HMW chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; hydrogen peroxide receptor and signal transducer in a light sensing pathway; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases and forms cytoplasmic foci during DNA replication stress
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:20385087|PMID:12464633]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802|PMID:22842922]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:genetic interaction:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:12464633]
EXISTENCE:mutant phenotype:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:12464633]
EXISTENCE:mutant phenotype:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:12464633|PMID:17314407]
Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
mitochondrial 54S ribosomal protein YmL6
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:9151978]
Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:3915776]
Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391]
EXISTENCE:direct assay:GO:0030915 Smc5-Smc6 complex [PMID:15738391]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:34191293]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391]
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
EXISTENCE:direct assay:GO:0003999 adenine phosphoribosyltransferase activity [PMID:9864350]
EXISTENCE:mutant phenotype:GO:0006168 adenine salvage [PMID:9864350]
Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004844 uracil DNA N-glycosylase activity [PMID:2644266]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11812822|PMID:24034606]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11812822|PMID:24034606]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:1938887]
Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus
dolichyl-diphosphooligosaccharide--protein glycotransferase
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084]
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:23416356]
EXISTENCE:genetic interaction:GO:0006487 protein N-linked glycosylation [PMID:10358084]
EXISTENCE:genetic interaction:GO:0065003 protein-containing complex assembly [PMID:10358084]
Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:22842922|PMID:26928762]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:7615085]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8824289|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0006883 cellular sodium ion homeostasis [PMID:7768897]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:21237705]
Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
TATA-binding protein-associated factor TAF11
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10818000]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:9083082]
TFIID subunit (40 kDa); involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
tRNA (carboxymethyluridine(34)-5-O)-methyltransferase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14645538]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14645538|PMID:14562095]
EXISTENCE:direct assay:GO:0016300 tRNA (uracil) methyltransferase activity [PMID:14645538]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:14645538]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0006448 regulation of translational elongation [PMID:27245397]
EXISTENCE:mutant phenotype:GO:0071466 cellular response to xenobiotic stimulus [PMID:14645538]
tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16179952]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:22454508|PMID:24868093]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16179953]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:31118508]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:31118508]
EXISTENCE:genetic interaction:GO:0034389 lipid droplet organization [PMID:22454508]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16179952]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:22454508]
EXISTENCE:mutant phenotype:GO:0034982 mitochondrial protein processing [PMID:31118508]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:15258615]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579]
EXISTENCE:physical interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16179952|PMID:16919153]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:16179952]
Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis; required for mitochondrial protein translocation-associated degradation
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:18283113]
Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:16595192]
Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:31289129]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:35102600]
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:26945063]
EXISTENCE:direct assay:GO:0001042 RNA polymerase I core binding [PMID:21467036]
EXISTENCE:direct assay:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:21467036]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:26945063]
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:27376968]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1840633]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:35102600]
EXISTENCE:direct assay:GO:0010508 positive regulation of autophagy [PMID:31462158]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:31289129]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:21467036]
EXISTENCE:direct assay:GO:0030619 U1 snRNA binding [PMID:31289129]
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:31289129]
EXISTENCE:direct assay:GO:0030621 U4 snRNA binding [PMID:31289129]
EXISTENCE:direct assay:GO:0030623 U5 snRNA binding [PMID:31289129]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:26945063]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:16908835]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:35102600]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:26945063]
EXISTENCE:direct assay:GO:0070990 snRNP binding [PMID:31289129]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:20042611]
EXISTENCE:genetic interaction:GO:2001208 negative regulation of transcription elongation by RNA polymerase I [PMID:21467039]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:20713510]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:19365074|PMID:26945063]
EXISTENCE:mutant phenotype:GO:0044877 protein-containing complex binding [PMID:26945063]
EXISTENCE:mutant phenotype:GO:2000232 regulation of rRNA processing [PMID:21949810]
EXISTENCE:mutant phenotype:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:21467039]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:9450930]
EXISTENCE:physical interaction:GO:0032044 DSIF complex [PMID:9450930]
Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and in concert with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; interacts with DNA upstream of RNAPII and the non-template strand of the transcription bubble; Spt5p is the only transcription elongation factor conserved in all domains of life
mitochondrial 54S ribosomal protein YmL39
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:2060626]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2060626]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:2060626]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0003838 sterol 24-C-methyltransferase activity [PMID:18563465|PMID:6363386]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11298754|PMID:9450962|PMID:6363386]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:6363386]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:2677674|PMID:6363386]
Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Mitochondrial hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
Upstream open reading frame (uORF) in 5' untranslated region of YAP1 gene, regulate translation
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:11056165]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11056165]
EXISTENCE:mutant phenotype:GO:0000304 response to singlet oxygen [PMID:16862604]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10809786]
EXISTENCE:mutant phenotype:GO:0061395 positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance [PMID:15575969]
EXISTENCE:mutant phenotype:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:27373166]
Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17030993]
EXISTENCE:genetic interaction:GO:0035556 intracellular signal transduction [PMID:10628841]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:17030993]
CAAX box containing hypothetical protein; proposed to be involved in the RAS/cAMP signaling pathway
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:16005430]
EXISTENCE:direct assay:GO:0016765 transferase activity, transferring alkyl or aryl (other than methyl) groups [PMID:16642040]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:16005430]
EXISTENCE:mutant phenotype:GO:0031591 wybutosine biosynthetic process [PMID:16642040|PMID:17150819]
S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family
EXISTENCE:direct assay:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:8824231]
EXISTENCE:mutant phenotype:GO:0004462 lactoylglutathione lyase activity [PMID:8824231]
EXISTENCE:mutant phenotype:GO:0006749 glutathione metabolic process [PMID:8824231]
Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; required for full activity of O-acetyl homoserine sulfhydrylase, Met17p; expression regulated by methylglyoxal levels and osmotic stress
ARS1309; Autonomously Replicating Sequence
Truncated 5' end of YML002W; in many strains, YML002W is continuous with YML003W, and is predicted to encode a single protein of 1090 aa with a full-length VPS9 domain; in strains S288C, W303, and CEN.PK, a single thymine residue is deleted near the 3' end of YML003W at ChrXIII:264337, causing a frameshift and premature stop codon, truncating YML003W
hypothetical protein; expression induced by heat and by calcium shortage; in many strains, YML002W is continuous with YML003W, and is predicted to encode a single protein of 1090 aa with a full-length VPS9 domain; in strains S288C, W303, and CEN.PK, a single thymine residue is deleted near the 3' end of YML003W at ChrXIII:264337, causing a frameshift and premature stop codon, truncating YML003W
EXISTENCE:direct assay:GO:0000011 vacuole inheritance [PMID:7489715]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11853670]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22593205|PMID:26928762]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0016192 vesicle-mediated transport [PMID:9015302]
EXISTENCE:direct assay:GO:0043254 regulation of protein-containing complex assembly [PMID:32160129]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:22593205]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026035]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:1473149]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:11598008|PMID:7489715]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:22593205]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:11062257|PMID:18809726|PMID:20604902|PMID:21325627]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:22593205]
Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:10594031]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:12637549|PMID:17122856|PMID:8321244|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:21498574]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9819423]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10594031]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:27226485]
EXISTENCE:direct assay:GO:0044732 mitotic spindle pole body [PMID:32553169]
EXISTENCE:direct assay:GO:0051219 phosphoprotein binding [PMID:27325700]
EXISTENCE:genetic interaction:GO:0010458 exit from mitosis [PMID:21937712]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:18500339]
EXISTENCE:genetic interaction:GO:0070194 synaptonemal complex disassembly [PMID:18832066]
EXISTENCE:genetic interaction:GO:1902542 regulation of protein localization to mitotic spindle pole body [PMID:21937712]
EXISTENCE:genetic interaction:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
EXISTENCE:mutant phenotype:GO:0000712 resolution of meiotic recombination intermediates [PMID:12717442]
EXISTENCE:mutant phenotype:GO:0004672 protein kinase activity [PMID:17122856|PMID:8321244]
EXISTENCE:mutant phenotype:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:18500339]
EXISTENCE:mutant phenotype:GO:0035025 positive regulation of Rho protein signal transduction [PMID:16763112]
EXISTENCE:mutant phenotype:GO:0090306 meiotic spindle assembly [PMID:20237423|PMID:24386320]
EXISTENCE:mutant phenotype:GO:0110083 positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly [PMID:16763112]
EXISTENCE:mutant phenotype:GO:1903353 regulation of nucleus organization [PMID:25454593]
EXISTENCE:mutant phenotype:GO:1904749 regulation of protein localization to nucleolus [PMID:33481703]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
EXISTENCE:physical interaction:GO:0044877 protein-containing complex binding [PMID:27226485|PMID:27325700]
Polo-like kinase required for mitotic exit; regulates mitotic spindle assembly, nuclear shape, and protein localization to the nucleolus and SPBs; controls targeting and activation of Rho1p at the division site via Rho1p-GEFs; role in adaptation to DNA damage promotes genome instability during replicative senescence; regulates meiotic commitment, spindle assembly, chiasmata formation, recombination intermediate resolution and SC disassembly; human homologs PLK1 and PLK3 complement cdc5 mutants
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:17095012]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:19703468]
Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
hypothetical protein; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21954159]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7862158]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:24567361]
EXISTENCE:direct assay:GO:0097320 plasma membrane tubulation [PMID:24567361]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:7862158]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:24567361]
Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with 'foot domain' of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12600943]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
TFIID subunit (48 kDa); involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0004622 lysophospholipase activity [PMID:10497163]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:10497163]
EXISTENCE:direct assay:GO:0006650 glycerophospholipid metabolic process [PMID:10497163]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953|PMID:15781460]
EXISTENCE:mutant phenotype:GO:0004622 lysophospholipase activity [PMID:10497163]
EXISTENCE:mutant phenotype:GO:0006650 glycerophospholipid metabolic process [PMID:10497163]
Phospholipase B (lysophospholipase) involved in lipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
hypothetical protein; conserved among S. cerevisiae strains; YMR007W is not an essential gene
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:25258318]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8051052|PMID:16622836]
EXISTENCE:direct assay:GO:0042597 periplasmic space [PMID:8051052]
EXISTENCE:mutant phenotype:GO:0004622 lysophospholipase activity [PMID:8051052]
EXISTENCE:mutant phenotype:GO:0006650 glycerophospholipid metabolic process [PMID:8051052]
EXISTENCE:mutant phenotype:GO:0036151 phosphatidylcholine acyl-chain remodeling [PMID:23501167]
Phospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication
Novel ORF identified via pan-genome analysis; conserved in other strains of S. cerevisiae
acireductone dioxygenase (Ni2+-requiring)
EXISTENCE:genetic interaction:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:15938715]
EXISTENCE:mutant phenotype:GO:0010308 acireductone dioxygenase (Ni2+-requiring) activity [PMID:15938715]
EXISTENCE:mutant phenotype:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:15938715|PMID:18625006]
Acireductone dioxygenease involved in methionine salvage pathway; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions; human ortholog ADI1 can complement yeast adi1 mutant
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:28057802]
EXISTENCE:direct assay:GO:0032588 trans-Golgi network membrane [PMID:28057802]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:28057802]
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:28057802]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:28057802]
Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene
ARS1310; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005355 glucose transmembrane transporter activity [PMID:12702270]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10191260|PMID:17507646|PMID:24124599]
EXISTENCE:mutant phenotype:GO:0015146 pentose transmembrane transporter activity [PMID:17180689]
EXISTENCE:mutant phenotype:GO:1904659 glucose transmembrane transport [PMID:2233722]
High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
translation initiation factor 3 subunit CLU1
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:7798228]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:2657387]
EXISTENCE:genetic interaction:GO:0004168 dolichol kinase activity [PMID:12213788]
EXISTENCE:genetic interaction:GO:0043048 dolichyl monophosphate biosynthetic process [PMID:12213788]
EXISTENCE:mutant phenotype:GO:0004168 dolichol kinase activity [PMID:2657387|PMID:12213788]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:2657387]
EXISTENCE:mutant phenotype:GO:0043048 dolichyl monophosphate biosynthetic process [PMID:12213788|PMID:1323123]
Dolichol kinase; catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
EXISTENCE:direct assay:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:9891049]
EXISTENCE:direct assay:GO:0031167 rRNA methylation [PMID:9891049]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:18726517|PMID:10024243|PMID:9891049]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:18726517|PMID:10024243|PMID:9891049]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U2421
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243|PMID:9891049]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243|PMID:9891049]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position U578
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position C2197
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position G2288
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position A28
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position C2959
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A876
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11452010]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:19806183]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:20498295]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:20498295|PMID:19806183]
Protein required for rRNA maturation and ribosomal subunit biogenesis; required for 18S rRNA maturation; also required for small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern
EXISTENCE:direct assay:GO:0000249 C-22 sterol desaturase activity [PMID:8543054]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:8543054]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:8635732]
C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:11046133|PMID:8524252]
Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:14742704]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:14742704]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14742704]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:14742704]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:10924463]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:14742704|PMID:27480805]
EXISTENCE:mutant phenotype:GO:0005628 prospore membrane [PMID:9425151]
EXISTENCE:mutant phenotype:GO:0031321 ascospore-type prospore assembly [PMID:9425151]
EXISTENCE:physical interaction:GO:0005484 SNAP receptor activity [PMID:10924463]
Meiosis-specific subunit of the t-SNARE complex; required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; binds to phosphatidic acid; SNAP-25 homolog
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27663510]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:27678487]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:27678487]
EXISTENCE:genetic interaction:GO:0016558 protein import into peroxisome matrix [PMID:27663510|PMID:27678487]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:27663510]
EXISTENCE:physical interaction:GO:0016558 protein import into peroxisome matrix [PMID:27678487]
Peroxisomal membrane signal receptor for peroxisomal matrix proteins; oleate-inducible condition-specific import receptor for a subset of PTS1-containing matrix proteins; localizes to both the cytosol and the peroxisomal membrane; similar to human PEX5Rp, a peroxin protein 5 related protein; paralog of Pex5p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay
EXISTENCE:direct assay:GO:0046592 polyamine oxidase activity [PMID:21067138]
EXISTENCE:genetic interaction:GO:0046208 spermine catabolic process [PMID:12586697]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
EXISTENCE:mutant phenotype:GO:0046208 spermine catabolic process [PMID:12586697]
EXISTENCE:mutant phenotype:GO:0046592 polyamine oxidase activity [PMID:11154694]
Polyamine oxidase; converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:9867833]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8262047]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9159110]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10887177]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:8262047|PMID:9211922]
EXISTENCE:mutant phenotype:GO:0045732 positive regulation of protein catabolic process [PMID:12011036]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8262047]
Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:9172777]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9388185]
EXISTENCE:direct assay:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:12105183]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:8641272]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:18971375]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:15643069]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:8641272|PMID:8781238]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29519818]
Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11604502]
EXISTENCE:mutant phenotype:GO:0070899 mitochondrial tRNA wobble uridine modification [PMID:15509579]
Mitochondrial protein; forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
mitochondrial 54S ribosomal protein YmL3
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type
EXISTENCE:direct assay:GO:0008180 COP9 signalosome [PMID:12446563]
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:12186635|PMID:12446563]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12446563]
Subunit of the Cop9 signalosome; which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:11370741]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:24034674]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:19687296|PMID:22471590]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:11370741|PMID:15536088|PMID:9090384]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12667447]
C3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:27322068]
A metal-dependent phosphatase, part of the DUF89 protein family; dephosphorylates fructose-1-phosphate; human ortholog, C6orf211 is involved in response to DNA damage; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16874307]
EXISTENCE:genetic interaction:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:19684113]
EXISTENCE:genetic interaction:GO:1903432 regulation of TORC1 signaling [PMID:10329624|PMID:14551259]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:19684113]
EXISTENCE:mutant phenotype:GO:1903432 regulation of TORC1 signaling [PMID:10604478|PMID:12820961]
Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23625923]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12734673]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12734673]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:12734673]
EXISTENCE:mutant phenotype:GO:0006390 mitochondrial transcription [PMID:12734673]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:12734673]
Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668|PMID:19269952|PMID:20526336]
EXISTENCE:genetic interaction:GO:0032126 eisosome [PMID:20526336]
EXISTENCE:mutant phenotype:GO:0070941 eisosome assembly [PMID:20526336]
Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:15775961|PMID:9864366|PMID:21498574|PMID:23468521|PM ID:24451263]
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:9864366|PMID:21498574|PMID:23468521|PMID:24451263]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:25620000]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:27385335|PMID:23468521]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:23468521]
EXISTENCE:direct assay:GO:0008092 cytoskeletal protein binding [PMID:18344988|PMID:9774458]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:23468521]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:23468521]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:32579428]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:32579428]
EXISTENCE:direct assay:GO:0051126 negative regulation of actin nucleation [PMID:30076201]
EXISTENCE:direct assay:GO:0120155 MIH complex [PMID:24895401]
EXISTENCE:genetic interaction:GO:0000281 mitotic cytokinesis [PMID:22623719]
EXISTENCE:genetic interaction:GO:0072741 protein localization to cell division site [PMID:23469085]
EXISTENCE:genetic interaction:GO:0090339 negative regulation of formin-nucleated actin cable assembly [PMID:24719456]
EXISTENCE:genetic interaction:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:23469085]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:9774458]
EXISTENCE:mutant phenotype:GO:0031671 primary cell septum biogenesis [PMID:29321253|PMID:21498574]
EXISTENCE:mutant phenotype:GO:0090339 negative regulation of formin-nucleated actin cable assembly [PMID:24719456]
EXISTENCE:mutant phenotype:GO:1902406 mitotic actomyosin contractile ring maintenance [PMID:9864366]
EXISTENCE:mutant phenotype:GO:1903471 regulation of mitotic actomyosin contractile ring contraction [PMID:24895401|PMID:21498574|PMID:23468521]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:23469085]
EXISTENCE:physical interaction:GO:0032038 myosin II heavy chain binding [PMID:23468521]
EXISTENCE:physical interaction:GO:0044697 HICS complex [PMID:22623719]
EXISTENCE:physical interaction:GO:0120155 MIH complex [PMID:24413167|PMID:24895401]
F-BAR protein that regulates actin cytoskeleton organization; binds and bundles actin filaments, linking them to septins; required for cytokinesis, actin cable organization, and secretory vesicle trafficking; regulates actomyosin ring dynamics and septin localization; N-term. half controls cell size and actin cable levels, while the C-term. half controls actin cable organization, inhibiting Bnr1p-mediated actin nucleation; forms axial striations/pillars at the bud neck; phosphorylated by Dbf2p
hypothetical protein; mCherry fusion protein localizes to the cytosol
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11011149]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8127913|PMID:9726966]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12183366]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9726966]
Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:26832117]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26832117]
EXISTENCE:genetic interaction:GO:0051481 negative regulation of cytosolic calcium ion concentration [PMID:26832117]
Negative regulator of cytosolic calcium homeostasis; localizes to the plasma membrane, concentrating at the bud neck during cell division; regulated by Crz1p through a CDRE promoter element; non-essential gene; putative transporter with sequence similarity to human SLC10A7; functional homolog of C. albicans RCH1
EXISTENCE:direct assay:GO:0004175 endopeptidase activity [PMID:8132591]
EXISTENCE:mutant phenotype:GO:0004175 endopeptidase activity [PMID:8266095]
EXISTENCE:mutant phenotype:GO:0006627 protein processing involved in protein targeting to mitochondrion [PMID:8266095]
EXISTENCE:physical interaction:GO:0042720 mitochondrial inner membrane peptidase complex [PMID:8132591|PMID:8266095]
Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificity), and Som1p
putative tyrosine protein phosphatase MIH1
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:2649252]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:2649252]
EXISTENCE:mutant phenotype:GO:0051231 spindle elongation [PMID:30072442]
Protein tyrosine phosphatase; involved in cell cycle control and retrograde protein transport; regulates the phosphorylation state of Cdc28p; modulates phosphorylation of retromer component Vps26p; homolog of S. pombe cdc25
stress-responsive transcriptional activator MSN2
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:23435728|PMID:8650168]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:8321194|PMID:8641288|PMID:8650168]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12732613|PMID:9472026|PMID:12093809]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:18070923]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:17914901]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:8650168]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11102521|PMID:15922872|PMID:16980434|PMID:8650168]
Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; light sensing pathway component that accumulates in the nucleus in response to blue light; relative distribution to nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18977757]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11500508]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9295278]
EXISTENCE:genetic interaction:GO:0016532 superoxide dismutase copper chaperone activity [PMID:9295278]
EXISTENCE:mutant phenotype:GO:0015680 protein maturation by copper ion transfer [PMID:9295278]
EXISTENCE:mutant phenotype:GO:0016532 superoxide dismutase copper chaperone activity [PMID:9295278]
Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within N-terminus is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress; human homolog CCS can complement yeast ccs1 null mutant
chromatin-binding transcription coactivator SUB1
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:26147804]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26147804|PMID:14562095]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19204085]
EXISTENCE:direct assay:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:19204085|PMID:19706510]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:25813861]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:19706510]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19204085]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:11389848]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:19204085]
EXISTENCE:genetic interaction:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:19204085]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:23554872]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19204085]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:15692559]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26837954]
EXISTENCE:mutant phenotype:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:19204085|PMID:19706510]
EXISTENCE:mutant phenotype:GO:0075297 negative regulation of ascospore formation [PMID:26147804]
Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4)
hypothetical protein that may interact with ribosomes; based on co-purification experiments; homolog of human BAP31 protein; YET2 has a paralog, YET1, that arose from the whole genome duplication
D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA2
EXISTENCE:direct assay:GO:0045290 D-arabinose 1-dehydrogenase [NAD(P)+] activity [PMID:17097644]
EXISTENCE:mutant phenotype:GO:0045290 D-arabinose 1-dehydrogenase [NAD(P)+] activity [PMID:17097644]
EXISTENCE:mutant phenotype:GO:0070485 dehydro-D-arabinono-1,4-lactone biosynthetic process [PMID:17097644]
NAD-dependent arabinose dehydrogenase; involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:10024171]
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:12138185]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:3311884|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:12138185]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8212891]
EXISTENCE:mutant phenotype:GO:1900079 regulation of arginine biosynthetic process [PMID:5434281]
EXISTENCE:mutant phenotype:GO:1900081 regulation of arginine catabolic process [PMID:12138185]
Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9121436]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12473677]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12738768|PMID:1752436|PMID:2673922]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:11562353]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9121436]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:12052870|PMID:9121436]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:12473677]
EXISTENCE:direct assay:GO:0044377 RNA polymerase II cis-regulatory region sequence-specific DNA binding, bending [PMID:12509445|PMID:7739902|PMID:9121436|PMID:9490409]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:19233144|PMID:9121436|PMID:11060038]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9490409]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8455631|PMID:16278448|PMID:1961765]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10594003]
EXISTENCE:mutant phenotype:GO:0031494 regulation of mating type switching [PMID:9620858]
EXISTENCE:mutant phenotype:GO:0044377 RNA polymerase II cis-regulatory region sequence-specific DNA binding, bending [PMID:10594003|PMID:12509445|PMID:15118075]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7565744|PMID:8455631|PMID:12738768|PMID:16278448|PMI D:19233144|PMID:1961765|PMID:9171372]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12052870|PMID:12711672]
EXISTENCE:physical interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:19233144]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12509445|PMID:12711672]
Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12482963]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12482963]
EXISTENCE:direct assay:GO:0036437 Isw1b complex [PMID:12482963]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:27185881]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:12482963]
Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
YMRCTy1-3; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:1385442]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:14960378]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:10952996]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327|PMID:17875746]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:8524308|PMID:9802895]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:8557738]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:9463388]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:8557738|PMID:9802895]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:11104765]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:12917401|PMID:17803941|PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:17483413]
EXISTENCE:genetic interaction:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714|PMID:17483413]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0043111 replication fork arrest [PMID:16103218|PMID:16418273]
EXISTENCE:mutant phenotype:GO:0043570 maintenance of DNA repeat elements [PMID:18321795]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0048478 replication fork protection [PMID:16418273]
Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0070545 PeBoW complex [PMID:16287855]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11522832]
EXISTENCE:physical interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:16287855]
EXISTENCE:physical interaction:GO:0070545 PeBoW complex [PMID:16287855]
Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; binds RNA via its C-terminal domain; homologous to mammalian Bop1
ARS1312; Autonomously Replicating Sequence
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
YMRCTy1-4; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:22782902]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23625923|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
hypothetical protein; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0070822 Sin3-type complex [PMID:9393435]
EXISTENCE:physical interaction:GO:0070822 Sin3-type complex [PMID:9393435]
Protein that interacts with Sin3p in a two-hybrid assay; part of a large protein complex with Sin3p and Stb1p; STB2 has a paralog, STB6, that arose from the whole genome duplication
H(+)-transporting V0 sector ATPase subunit a
EXISTENCE:direct assay:GO:0000220 vacuolar proton-transporting V-type ATPase, V0 domain [PMID:7514599]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11592965]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:11948198|PMID:11592965]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:28720663]
EXISTENCE:genetic interaction:GO:0007035 vacuolar acidification [PMID:11278748|PMID:7514599]
EXISTENCE:genetic interaction:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:7514599]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:11278748]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:28720663]
Subunit a of vacuolar-ATPase V0 domain; encodes one of two isoforms, located in both Golgi and endosomal V-ATPase complexes while VPH1 encodes second isoform and is located in vacuolar V-ATPase complexes; interaction with PI(4)P is required for efficient localization and function of Stv1-containing V-ATPases; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12048186|PMID:18667533|PMID:25658911]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10220406|PMID:10352016]
EXISTENCE:direct assay:GO:1990334 Bfa1-Bub2 complex [PMID:25658911]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:25658911]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:16039591|PMID:25658911]
EXISTENCE:mutant phenotype:GO:1902543 negative regulation of protein localization to mitotic spindle pole body [PMID:25658911]
EXISTENCE:physical interaction:GO:1990334 Bfa1-Bub2 complex [PMID:11493673]
Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
EXISTENCE:direct assay:GO:0005471 ATP:ADP antiporter activity [PMID:2167309]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:22842922|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12740376]
EXISTENCE:genetic interaction:GO:0006783 heme biosynthetic process [PMID:18728780]
EXISTENCE:genetic interaction:GO:0006839 mitochondrial transport [PMID:2167309]
EXISTENCE:genetic interaction:GO:0009060 aerobic respiration [PMID:2167309]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:18728780]
Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0004322 ferroxidase activity [PMID:8599111|PMID:9722559]
EXISTENCE:direct assay:GO:0005381 iron ion transmembrane transporter activity [PMID:16700543]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16700543|PMID:9162052]
EXISTENCE:direct assay:GO:0033573 high-affinity iron permease complex [PMID:16522632]
EXISTENCE:genetic interaction:GO:0034755 iron ion transmembrane transport [PMID:8599111]
EXISTENCE:genetic interaction:GO:1901684 arsenate ion transmembrane transport [PMID:23295455]
EXISTENCE:mutant phenotype:GO:0033215 reductive iron assimilation [PMID:16700543]
EXISTENCE:mutant phenotype:GO:0034755 iron ion transmembrane transport [PMID:16700543|PMID:8293473|PMID:8599111]
EXISTENCE:mutant phenotype:GO:0046688 response to copper ion [PMID:12954629]
Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000213 tRNA-intron endonuclease activity [PMID:9200603]
EXISTENCE:direct assay:GO:0000214 tRNA-intron endonuclease complex [PMID:9200603]
EXISTENCE:direct assay:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:9200603]
Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface
EXISTENCE:direct assay:GO:0001401 SAM complex [PMID:12891361]
EXISTENCE:mutant phenotype:GO:0015914 phospholipid transport [PMID:21299651]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:18039934]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:12891361|PMID:18039934]
Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
cleavage polyadenylation factor subunit RNA14
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15215336]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10660071|PMID:22932476]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10660071]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0072423 response to DNA damage checkpoint signaling [PMID:24603480]
EXISTENCE:physical interaction:GO:0005849 mRNA cleavage factor complex [PMID:11344258]
Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0004042 acetyl-CoA:L-glutamate N-acetyltransferase activity [PMID:9428669]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:205532]
EXISTENCE:direct assay:GO:0006592 ornithine biosynthetic process [PMID:9428669]
Mitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:33002606]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23019326]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:8417990]
Plasma membrane hypothetical protein; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:21454598]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:21454598]
Protein required for expression of the mitochondrial OLI1 gene; mitochondrial OLI1 gene encodes subunit 9 of F1-F0 ATP synthase
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9382856]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:10069807]
Protein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0008494 translation activator activity [PMID:32449921]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:32449921]
EXISTENCE:mutant phenotype:GO:0061668 mitochondrial ribosome assembly [PMID:32449921]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14755638|PMID:14562095]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:19359248]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:21148305]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:19359248]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:14755638]
UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the role of the Cdc48p-Nplp-Ufd1p AAA ATPase complex in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress; human ortholog ASPSCR1 partially complements the yeast null mutant
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:30969890]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:12408816]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:physical interaction:GO:0031932 TORC2 complex [PMID:12408816]
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; PM retention is dependent on Avo3p
N-terminal L-serine N(alpha)-acetyltransferase NatD
EXISTENCE:direct assay:GO:0010485 histone H4 acetyltransferase activity [PMID:12915400]
EXISTENCE:direct assay:GO:0043967 histone H4 acetylation [PMID:12915400]
EXISTENCE:mutant phenotype:GO:0010485 histone H4 acetyltransferase activity [PMID:12915400]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:24068969]
EXISTENCE:mutant phenotype:GO:0043967 histone H4 acetylation [PMID:12915400]
EXISTENCE:mutant phenotype:GO:0043968 histone H2A acetylation [PMID:12915400]
EXISTENCE:mutant phenotype:GO:0043998 histone H2A acetyltransferase activity [PMID:12915400]
N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9611199|PMID:10982825]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:23435728]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12145211|PMID:10982825|PMID:21299653]
EXISTENCE:mutant phenotype:GO:0010895 negative regulation of ergosterol biosynthetic process [PMID:12145211|PMID:21299653]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11292809|PMID:23435728]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:14665463]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:16783004]
Transcriptional repressor, activator; role in cellular adjustment to osmotic stress including modulation of mating efficiency; involved in repression of subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; relocalizes to cytosol under hypoxia; forms [MOT3+] prion under anaerobic conditions
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:16107716]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17178117]
Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport
EXISTENCE:direct assay:GO:0000262 mitochondrial chromosome [PMID:9581629]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:27449618|PMID:9581629]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:1737790]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431|PMID:27371858]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:9581629]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:9581629]
EXISTENCE:mutant phenotype:GO:0090139 mitochondrial chromosome packaging [PMID:21282901]
Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:23273983]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:16121259]
hypothetical protein; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:19469552]
Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000118 histone deacetylase complex [PMID:16286008]
EXISTENCE:direct assay:GO:0032221 Rpd3S/Clr6-CII complex [PMID:16286007|PMID:16286008]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:19948887]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:19417103]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24358376]
EXISTENCE:mutant phenotype:GO:0060195 negative regulation of antisense RNA transcription [PMID:21248844]
Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
hypothetical protein; almost completely overlaps verified ORF RCO1/YMR075W
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:11062262]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:22842922]
EXISTENCE:direct assay:GO:0035808 meiotic recombination initiation complex [PMID:31617566]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:11137006]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11448778|PMID:19736318]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:11062262|PMID:14623866]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:11062262]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:19736318]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:35102525]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:31617566]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:15819623]
EXISTENCE:mutant phenotype:GO:0140588 chromatin looping [PMID:32032532]
Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; regulates homolog pairing and facilitates synaptonemal complex formation, axis formation, interhomolog recombination and synapsis during meiosis; relocalizes to the cytosol in response to hypoxia; scaffolding protein contains 26 HEAT repeats
EXISTENCE:direct assay:GO:0000815 ESCRT III complex [PMID:12194857]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12194857]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:31273031]
EXISTENCE:genetic interaction:GO:0070676 intralumenal vesicle formation [PMID:23444383]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:12953057]
EXISTENCE:mutant phenotype:GO:0070676 intralumenal vesicle formation [PMID:19234443]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15086794]
Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16962805]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:17483413]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:17636314]
EXISTENCE:mutant phenotype:GO:0003689 DNA clamp loader activity [PMID:32277910]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:20980819]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:11287619|PMID:11389843]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:16525505]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:21347277]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
phosphatidylinositol/phosphatidylcholine transfer protein SEC14
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:7816798]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1997207]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:1997207]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12869188|PMID:2466847|PMID:26928762]
EXISTENCE:direct assay:GO:0008525 phosphatidylcholine transporter activity [PMID:10488334|PMID:10848624|PMID:2407740]
EXISTENCE:direct assay:GO:0008526 phosphatidylinositol transfer activity [PMID:10848624|PMID:2407740|PMID:2215682]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:10848624|PMID:2407740|PMID:2215682]
EXISTENCE:direct assay:GO:0048194 Golgi vesicle budding [PMID:9553090|PMID:9736710]
EXISTENCE:direct assay:GO:2001246 negative regulation of phosphatidylcholine biosynthetic process [PMID:7816798]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:18753406]
EXISTENCE:genetic interaction:GO:0046488 phosphatidylinositol metabolic process [PMID:10567405]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:18753406]
EXISTENCE:mutant phenotype:GO:0008525 phosphatidylcholine transporter activity [PMID:10488334]
EXISTENCE:mutant phenotype:GO:0008526 phosphatidylinositol transfer activity [PMID:10848624|PMID:2215682]
EXISTENCE:mutant phenotype:GO:0015914 phospholipid transport [PMID:2215682]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:14528019]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:10848624|PMID:19129178|PMID:6754086]
EXISTENCE:mutant phenotype:GO:0046488 phosphatidylinositol metabolic process [PMID:10567405]
EXISTENCE:mutant phenotype:GO:1901352 negative regulation of phosphatidylglycerol biosynthetic process [PMID:12869188]
EXISTENCE:mutant phenotype:GO:2001246 negative regulation of phosphatidylcholine biosynthetic process [PMID:7816798]
Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:8816462]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:7545033]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:18676617]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:8816461|PMID:8816462]
EXISTENCE:direct assay:GO:0043024 ribosomal small subunit binding [PMID:23801788]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16157874]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12769863|PMID:12881429]
EXISTENCE:mutant phenotype:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:8816461|PMID:8816462]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:16285926]
EXISTENCE:mutant phenotype:GO:0006449 regulation of translational termination [PMID:8816461]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:16777600]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:18676617]
EXISTENCE:mutant phenotype:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:physical interaction:GO:0016567 protein ubiquitination [PMID:18676617]
ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:genetic interaction:GO:0009060 aerobic respiration [PMID:8208248]
Serine-rich, hydrophilic protein; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; ISF1 has a paralog, MBR1, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YMR082C is not an essential gene
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:3550419]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:3550419]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:3546317]
EXISTENCE:genetic interaction:GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
putative glutamine--fructose-6-phosphate transaminase
hypothetical protein; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
hypothetical protein; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19269952|PMID:22869600]
EXISTENCE:mutant phenotype:GO:0070941 eisosome assembly [PMID:22869600]
Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication
putative ADP-ribose 1''-phosphate phosphatase
Putative ADP-ribose-1''-monophosphatase; converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15572352|PMID:26928762]
EXISTENCE:direct assay:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015802 basic amino acid transport [PMID:15572352]
Permease of basic amino acids in vacuolar membrane; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:19748354]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7929327]
EXISTENCE:direct assay:GO:0005745 m-AAA complex [PMID:8681382]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:19748354]
EXISTENCE:direct assay:GO:0097002 mitochondrial inner boundary membrane [PMID:19019989]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:12417197]
EXISTENCE:mutant phenotype:GO:0008237 metallopeptidase activity [PMID:8681382]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:19748354]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:8681382]
Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation
hypothetical protein; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366|PMID:14729968]
Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:10366597]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:23333351]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10366597]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:genetic interaction:GO:0030042 actin filament depolymerization [PMID:10366597]
EXISTENCE:genetic interaction:GO:0032466 negative regulation of cytokinesis [PMID:25371407]
EXISTENCE:mutant phenotype:GO:0051016 barbed-end actin filament capping [PMID:23333351]
EXISTENCE:physical interaction:GO:0030042 actin filament depolymerization [PMID:10366597]
Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; mutations in human homolog WDR1 are associated with cardiac hypertrophy
snoRNA-binding rRNA-processing protein UTP15
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:10097110]
EXISTENCE:direct assay:GO:0031518 CBF3 complex [PMID:1997204|PMID:8670864|PMID:8706132]
EXISTENCE:direct assay:GO:0051382 kinetochore assembly [PMID:12084919]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:16330709]
Subunit of the CBF3 complex; F-box protein of the leucine-rich-repeat family; required for assembly of the complex; CBF3 binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
putative pyridoxal 5'-phosphate synthase
EXISTENCE:direct assay:GO:0004359 glutaminase activity [PMID:14764090]
EXISTENCE:genetic interaction:GO:0008615 pyridoxine biosynthetic process [PMID:14764090]
EXISTENCE:mutant phenotype:GO:0036001 'de novo' pyridoxal 5'-phosphate biosynthetic process [PMID:30498136]
EXISTENCE:mutant phenotype:GO:0042819 vitamin B6 biosynthetic process [PMID:12271461]
EXISTENCE:mutant phenotype:GO:0043066 negative regulation of apoptotic process [PMID:18355271]
EXISTENCE:physical interaction:GO:1903600 glutaminase complex [PMID:14764090]
Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
EXISTENCE:direct assay:GO:0036381 pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity [PMID:19523954]
EXISTENCE:genetic interaction:GO:0008615 pyridoxine biosynthetic process [PMID:14764090|PMID:30498136]
EXISTENCE:mutant phenotype:GO:0008615 pyridoxine biosynthetic process [PMID:30498136]
Subunit of a two-component pyridoxal-5'-phosphate synthase; involved in pyridoxine (vitamin B6) biosynthesis from glyceraldehyde-3-phosphate, ribose-5-phosphate and ammonia liberated from glutamine by glutaminase; forms a complex with Sno1p that functions as an active glutaminase; member of a stationary phase-induced gene family where transcriptional induction occurs during the postdiauxic phase; co-regulated with SNO1; paralog of SNZ2 and SNZ3
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12808030]
EXISTENCE:genetic interaction:GO:0032543 mitochondrial translation [PMID:12808030]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:12808030]
Putative GTPase peripheral to the mitochondrial inner membrane; essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:18216280]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:18216280]
EXISTENCE:mutant phenotype:GO:0048255 mRNA stabilization [PMID:18216280]
Protein that associates with mitochondrial ribosome; required for the stability of Oli1p (Atp9p) mRNA; also required for the Oli1p ring formation; YMR098C is not an essential gene
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:curator inference:GO:0044262 cellular carbohydrate metabolic process [PMID:16857670]
EXISTENCE:direct assay:GO:0047938 glucose-6-phosphate 1-epimerase activity [PMID:16857670]
Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase); likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:18070918]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:18070918]
EXISTENCE:physical interaction:GO:1990304 MUB1-RAD6-UBR2 ubiquitin ligase complex [PMID:18070918]
MYND domain-containing protein; component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex, required for ubiquitination and degradation of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; proposed to function as both a partner and substrate of the Ubr2p/Rad6p ubiquitin ligase; similar to the A. nidulans samB gene
ARS1316; Autonomously Replicating Sequence
ditrans,polycis-polyprenyl diphosphate synthase
EXISTENCE:direct assay:GO:0004659 prenyltransferase activity [PMID:17345630]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:11442630|PMID:24390141]
EXISTENCE:direct assay:GO:0006486 protein glycosylation [PMID:11442630]
EXISTENCE:direct assay:GO:0019408 dolichol biosynthetic process [PMID:25066056]
EXISTENCE:direct assay:GO:0045547 dehydrodolichyl diphosphate synthase activity [PMID:11442630|PMID:25066056]
EXISTENCE:direct assay:GO:1904423 dehydrodolichyl diphosphate synthase complex [PMID:25066056]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:11442630]
Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:33255682]
Sterol-binding beta-propeller protein; physically associates with sterol-binding StARkin domain-containing proteins Ysp2/Lam2/Ltc4 and Lam4/Ltc3; essential for retrograde transport of ergosterol from the plasma membrane to the endoplasmic reticulum at ER-PM contact sites; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; non-essential gene; has a paralog, DGR2, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YMR103C is not an essential gene
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:16478726]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12221112]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:12221112]
EXISTENCE:genetic interaction:GO:0070941 eisosome assembly [PMID:18296441]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:16478726]
AGC-type S/T protein kinase; participates in the cell wall integrity signaling pathway; involved with Ypk1p in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6), and in the direct phosphorylation of the Myo5p motor domain TEDS site; phosphorylated and activated by Pkh1/2p in the presence of phytosphingosine; direct phosphorylation by Tor2p regulates actin polarization; genetically redundant with YPK1; human homolog SGK2 can complement the inviability of a ypk1 ypk2 mutant
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8385141]
EXISTENCE:genetic interaction:GO:0004614 phosphoglucomutase activity [PMID:8119301]
EXISTENCE:genetic interaction:GO:0005978 glycogen biosynthetic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0005992 trehalose biosynthetic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0006011 UDP-glucose metabolic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0006874 cellular calcium ion homeostasis [PMID:15252028]
EXISTENCE:genetic interaction:GO:0019255 glucose 1-phosphate metabolic process [PMID:8119301]
EXISTENCE:genetic interaction:GO:0019388 galactose catabolic process [PMID:8119301]
EXISTENCE:genetic interaction:GO:0051156 glucose 6-phosphate metabolic process [PMID:8119301]
EXISTENCE:mutant phenotype:GO:0004614 phosphoglucomutase activity [PMID:2138705|PMID:8119301|PMID:13887540]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:10681519|PMID:15252028]
EXISTENCE:mutant phenotype:GO:0019255 glucose 1-phosphate metabolic process [PMID:10681519|PMID:8119301]
EXISTENCE:mutant phenotype:GO:0019388 galactose catabolic process [PMID:8119301|PMID:13887540|PMID:14264884]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:15164360]
Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
ARS1317; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16166630]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:11553718]
EXISTENCE:direct assay:GO:0007535 donor selection [PMID:16166630]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:17656141]
EXISTENCE:direct assay:GO:0043564 Ku70:Ku80 complex [PMID:8754818]
EXISTENCE:direct assay:GO:0070034 telomerase RNA binding [PMID:12975323]
EXISTENCE:genetic interaction:GO:0007535 donor selection [PMID:16809780]
EXISTENCE:genetic interaction:GO:0034502 protein localization to chromosome [PMID:18716325]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:9501103]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:11016833]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:27122604]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:9501103]
EXISTENCE:mutant phenotype:GO:0007535 donor selection [PMID:16166630|PMID:16809780]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:9501103]
EXISTENCE:mutant phenotype:GO:0097695 establishment of protein-containing complex localization to telomere [PMID:15531893]
Subunit of telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair; colocalizes with quiescent cell telomere hyperclusters
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources
EXISTENCE:direct assay:GO:0003984 acetolactate synthase activity [PMID:10213630]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005948 acetolactate synthase complex [PMID:10213630]
EXISTENCE:direct assay:GO:0009082 branched-chain amino acid biosynthetic process [PMID:2406721]
EXISTENCE:direct assay:GO:0050660 flavin adenine dinucleotide binding [PMID:15709745]
EXISTENCE:mutant phenotype:GO:0009082 branched-chain amino acid biosynthetic process [PMID:2406721]
Acetolactate synthase; catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control
EXISTENCE:direct assay:GO:0000146 microfilament motor activity [PMID:16824951]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:16824951|PMID:20647997]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:16824951]
EXISTENCE:genetic interaction:GO:0009651 response to salt stress [PMID:12391157]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16824951]
EXISTENCE:mutant phenotype:GO:0006898 receptor-mediated endocytosis [PMID:8614799]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:16824951]
EXISTENCE:physical interaction:GO:0009651 response to salt stress [PMID:12391157]
One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006081 cellular aldehyde metabolic process [PMID:22633490]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:22633490]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:27669165]
EXISTENCE:mutant phenotype:GO:0018484 4-hydroxybenzaldehyde dehydrogenase activity [PMID:27693056|PMID:33862086|PMID:27669165]
EXISTENCE:mutant phenotype:GO:0032180 ubiquinone biosynthetic process from tyrosine [PMID:27693056|PMID:33862086]
EXISTENCE:mutant phenotype:GO:0046185 aldehyde catabolic process [PMID:25545362]
EXISTENCE:mutant phenotype:GO:0047770 carboxylate reductase activity [PMID:22633490]
Dehydrogenase involved in ubiquinone and sphingolipid metabolism; converts 4-hydroxybenzaldehyde into 4-hydroxybenzoate for ubiquinone anabolism, hexadecenal to hexadecenoic acid in sphingosine 1-phosphate catabolism; human homolog ALDH3A2, mutated in Sjogren-Larsson syndrome, can rescue yeast hfd1 mutant; human ALDH3A1, but not ALDH3A2, rescues pABA- respiratory growth phenotype of hfd1 null; data suggest that dual functions of Hfd1p have diverged in human ALDH3A1, ALDH3A2
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:31015336]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:31015336]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:31015336]
Sequence-specific DNA binding protein; binds to a sequence motif associated with distinct genomic hotspots of non-H2B protein ubiquitylation (ub-hotspots); interacts with Slx5p to recruit the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex to ub-hotspots; deletion prevents ub-hotspot formation and exacerbates heat sensitivity of cells deficient in Rpd3L histone deacetylase complex members; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0001097 TFIIH-class transcription factor complex binding [PMID:18691966]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:18691966]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:18691966]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
EXISTENCE:mutant phenotype:GO:0008841 dihydrofolate synthase activity [PMID:11731153]
EXISTENCE:mutant phenotype:GO:0009396 folic acid-containing compound biosynthetic process [PMID:11731153]
Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:24390141]
hypothetical protein; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:18843051]
EXISTENCE:direct assay:GO:0031942 i-AAA complex [PMID:18843051]
EXISTENCE:direct assay:GO:0051787 misfolded protein binding [PMID:18843051]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:18843051]
Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
guanine nucleotide-binding protein subunit beta
EXISTENCE:direct assay:GO:0005092 GDP-dissociation inhibitor activity [PMID:17591772]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:15340087]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27821475]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:17591772]
EXISTENCE:mutant phenotype:GO:0001934 positive regulation of protein phosphorylation [PMID:27821475]
EXISTENCE:mutant phenotype:GO:0006521 regulation of cellular amino acid metabolic process [PMID:34214075]
EXISTENCE:mutant phenotype:GO:0007186 G protein-coupled receptor signaling pathway [PMID:17591772]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:31689955]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:31532761]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:15340087]
EXISTENCE:mutant phenotype:GO:0061157 mRNA destabilization [PMID:31532761]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:28956756]
EXISTENCE:mutant phenotype:GO:0080135 regulation of cellular response to stress [PMID:31689955]
EXISTENCE:mutant phenotype:GO:1902660 negative regulation of glucose mediated signaling pathway [PMID:17591772]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:30465652]
EXISTENCE:mutant phenotype:GO:2001125 negative regulation of translational frameshifting [PMID:25792604]
EXISTENCE:physical interaction:GO:0001965 G-protein alpha-subunit binding [PMID:17591772]
EXISTENCE:physical interaction:GO:0007186 G protein-coupled receptor signaling pathway [PMID:17591772]
EXISTENCE:physical interaction:GO:1902660 negative regulation of glucose mediated signaling pathway [PMID:17591772]
G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; required to prevent frameshifting at ribosomes stalled at repeated CGA codons; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation; controls phosphorylation of multiple proteins
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:8674114]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:8674114]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
C/D box small nucleolar RNA (snoRNA); commonly referred to as U24; guides 2'-O-methylation of large subunit (LSU) rRNA at positions C1437, C1449, and C1450
kinetochore-associated Ndc80 complex subunit SPC24
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11511347]
EXISTENCE:direct assay:GO:0031262 Ndc80 complex [PMID:11179222|PMID:11266451]
EXISTENCE:mutant phenotype:GO:0000776 kinetochore [PMID:11179222]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11266451]
EXISTENCE:mutant phenotype:GO:0098653 centromere clustering [PMID:11179222]
Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
Putative mitochondrial inner membrane hypothetical protein; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication
ARS1319; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:16735580|PMID:27831485]
EXISTENCE:direct assay:GO:0097658 Asi complex [PMID:25236469]
EXISTENCE:genetic interaction:GO:0004842 ubiquitin-protein transferase activity [PMID:11454748]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11454748]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:25236469]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25236469]
EXISTENCE:mutant phenotype:GO:0036369 transcription factor catabolic process [PMID:25253722]
EXISTENCE:mutant phenotype:GO:0071230 cellular response to amino acid stimulus [PMID:17085444]
Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17
EXISTENCE:direct assay:GO:0003937 IMP cyclohydrolase activity [PMID:10877846]
EXISTENCE:direct assay:GO:0004643 phosphoribosylaminoimidazolecarboxamide formyltransferase activity [PMID:10877846]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10877846]
EXISTENCE:direct assay:GO:0006164 purine nucleotide biosynthetic process [PMID:10877846]
EXISTENCE:direct assay:GO:0006189 'de novo' IMP biosynthetic process [PMID:10877846]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:10877846]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:10877846]
Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:6814480]
Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains; YMR122C is not an essential gene
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and endoplasmic reticulum
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16926153]
EXISTENCE:genetic interaction:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:16926153]
EXISTENCE:mutant phenotype:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:16926153]
V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0061163 endoplasmic reticulum polarization [PMID:25083872]
EXISTENCE:physical interaction:GO:0005935 cellular bud neck [PMID:25083872]
Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:15753296]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:22072789]
EXISTENCE:direct assay:GO:0005846 nuclear cap binding complex [PMID:8811086]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:22072789]
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
hypothetical protein; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
EXISTENCE:curator inference:GO:0000781 chromosome, telomeric region [PMID:11731479]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11731479]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:12626510]
EXISTENCE:direct assay:GO:0016407 acetyltransferase activity [PMID:11731479]
EXISTENCE:direct assay:GO:0031509 subtelomeric heterochromatin formation [PMID:11731479]
EXISTENCE:direct assay:GO:0033255 SAS acetyltransferase complex [PMID:12626510]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:27655944]
EXISTENCE:physical interaction:GO:0033255 SAS acetyltransferase complex [PMID:15788653]
Histone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:10982841]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10982841|PMID:22842922]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:10982841]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16449634]
Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; functions with methyltransferase Bud23p; predominantly nucleolar in distribution; required for 18S rRNA synthesis
EXISTENCE:curator inference:GO:0006913 nucleocytoplasmic transport [PMID:8138573]
EXISTENCE:direct assay:GO:0005641 nuclear envelope lumen [PMID:9988776]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:7559775|PMID:8138573|PMID:9988776]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:7559775]
EXISTENCE:direct assay:GO:0043495 protein-membrane adaptor activity [PMID:7559775]
EXISTENCE:direct assay:GO:0070762 nuclear pore transmembrane ring [PMID:10684247|PMID:7559775|PMID:19414609]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:19414609]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:20713690]
Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; type II transmembrane protein composed of N-terminal cytosolic and C-terminal nuclear envelope lumenal domains; homologous to human NUP210
Putative mitochondrial hypothetical protein; DPI35 is not an essential gene
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10684247]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:26112308]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:11728313]
Specific assembly chaperone for ribosomal protein Rpl3p; co-translationally associates with nascent Rpl3p, preventing aggregation, and facilitating nuclear import; required for biogenesis of preribosomal particles; essential gene; contains five WD-repeat motifs
hypothetical protein; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
ARS1320; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16783010|PMID:17558514]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:8417989]
Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:14690591]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:14690591|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:23892078|PMID:29773647]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9180083]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:16135527]
hypothetical protein involved in ergosterol biosynthesis; conditional mutants produce less ergosterol, display impaired oxygen consumption, respiratory growth, mitochondrial iron utilization, and are more sensitive to oxidative stress; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; protein localizes to the cytoplasm, ER and nuclear envelope; highly conserved in ascomycetes
glucose-induced degradation complex subunit GID8
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:16872538|PMID:18508925]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:15590836]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
ARS1321; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:35482533]
EXISTENCE:direct assay:GO:0008409 5'-3' exonuclease activity [PMID:15590324|PMID:35482533]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0003684 damaged DNA binding [PMID:7035927]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:1736091|PMID:35482533]
EXISTENCE:mutant phenotype:GO:0008409 5'-3' exonuclease activity [PMID:15590324]
EXISTENCE:mutant phenotype:GO:0036297 interstrand cross-link repair [PMID:22912599]
Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress; translocates to mitochondria under genotoxic agents
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:2407611]
EXISTENCE:genetic interaction:GO:0007021 tubulin complex assembly [PMID:9215891]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9215891]
GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:9372955]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:10679022]
Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:10628972]
hypothetical protein; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains; YMR141C is not an essential gene
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0006413 translational initiation [PMID:30481328]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0007535 donor selection [PMID:27257873]
EXISTENCE:physical interaction:GO:0007535 donor selection [PMID:27257873]
Protein involved in directionality of mating type switching; acts with Fkh1p to control which donor mating-type locus is inserted into MAT locus during mating type switching; localized to the nucleus; not an essential gene
ARS1322; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
NADH-ubiquinone reductase (H(+)-translocating) NDE1
EXISTENCE:direct assay:GO:0003954 NADH dehydrogenase activity [PMID:9696750|PMID:9733747]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:12032156]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:31668496]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9696750|PMID:9733747]
EXISTENCE:mutant phenotype:GO:0019655 glycolytic fermentation to ethanol [PMID:9696750|PMID:9733747]
EXISTENCE:mutant phenotype:GO:0042981 regulation of apoptotic process [PMID:31668496]
Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication
translation initiation factor eIF3 subunit i
EXISTENCE:direct assay:GO:0002183 cytoplasmic translational initiation [PMID:7798228]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:9671501]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9671501|PMID:9660829]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:9671501]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:29192585]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:11018020|PMID:15838098]
EXISTENCE:mutant phenotype:GO:0002188 translation reinitiation [PMID:20679478]
EXISTENCE:physical interaction:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9660829]
eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; stimulates rate of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:29187528]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:29187528]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:29187528]
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cell periphery
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:19779569]
hypothetical protein with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
dolichyl-diphosphooligosaccharide-protein glycotransferase
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:29466327]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:29466327]
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:29466327]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:9405463]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Delta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum
EXISTENCE:direct assay:GO:0004175 endopeptidase activity [PMID:1991446|PMID:8132591]
EXISTENCE:direct assay:GO:0006627 protein processing involved in protein targeting to mitochondrion [PMID:1991446]
EXISTENCE:physical interaction:GO:0042720 mitochondrial inner membrane peptidase complex [PMID:8266095]
Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificty) and Som1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0018455 alcohol dehydrogenase [NAD(P)+] activity [PMID:32967812]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:12077312]
Aldehyde reductase; involved in detoxification of lignocellulose-derived aldehydes; catalyzes the reduction of furan, aliphatic and aromatic aldehydes using either NADH or NADPH as co-factor; membrane of the quinone oxidoreductase subfamily of the medium-chain dehydrogenase/reductase family; null mutant displays sensitivity to DNA damaging agents; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:11071914|PMID:9864357]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:11071914]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:9864357]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:11071914]
EXISTENCE:genetic interaction:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:11071914]
EXISTENCE:mutant phenotype:GO:0007088 regulation of mitotic nuclear division [PMID:14697200]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:12473689]
EXISTENCE:mutant phenotype:GO:0060188 regulation of protein desumoylation [PMID:17403926]
EXISTENCE:mutant phenotype:GO:0072417 response to spindle checkpoint signaling [PMID:12473689]
EXISTENCE:physical interaction:GO:0007088 regulation of mitotic nuclear division [PMID:14697200]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17418788|PMID:9864357]
EXISTENCE:physical interaction:GO:0060188 regulation of protein desumoylation [PMID:17403926]
FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004197 cysteine-type endopeptidase activity [PMID:9928935]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:9928935]
Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
hypothetical protein; identified as interacting with Hsp82p in a high-throughput two-hybrid screen
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:11278831|PMID:15222769]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:15222769]
EXISTENCE:direct assay:GO:0046403 polynucleotide 3'-phosphatase activity [PMID:11278831]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:11585902]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11585902]
EXISTENCE:mutant phenotype:GO:0046403 polynucleotide 3'-phosphatase activity [PMID:15222769]
DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3'-phosphatase
hypothetical protein; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
mitochondrial 37S ribosomal protein MRPS8
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the small subunit
Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
hypothetical protein; may contain a lipid attachment site; localizes to cytosol, and to peroxisomes in oleate-growing cells; YMR158C-A is not an essential gene
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:11689437]
EXISTENCE:direct assay:GO:0016236 macroautophagy [PMID:11897782]
EXISTENCE:direct assay:GO:0034274 Atg12-Atg5-Atg16 complex [PMID:11897782]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:direct assay:GO:0120095 vacuole-isolation membrane contact site [PMID:23549786]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0006501 C-terminal protein lipidation [PMID:17192262]
EXISTENCE:mutant phenotype:GO:0019776 Atg8 ligase activity [PMID:17192262]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:11897782]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:17192262]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:1905037 autophagosome organization [PMID:24485455]
EXISTENCE:physical interaction:GO:0034274 Atg12-Atg5-Atg16 complex [PMID:10406794]
Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:35766971]
EXISTENCE:direct assay:GO:0098853 endoplasmic reticulum-vacuole membrane contact site [PMID:35766971]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:35766971]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:35766971]
Protein involved in sphingolipid metabolism; localizes to vacuolar contact sites with mitochondria, ER and peroxisomes; mutant has enhanced sensitivity to overexpression of mutant huntingtin; relative distribution within the vacuolar membrane changes upon DNA replication stress
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:15342786]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12514182]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:15252059|PMID:15342786]
Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
aminophospholipid-translocating P4-type ATPase DNF3
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:17093059]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:32661085]
EXISTENCE:direct assay:GO:0030140 trans-Golgi network transport vesicle [PMID:12221123]
EXISTENCE:direct assay:GO:0070867 mating projection tip membrane [PMID:25378585]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0090555 phosphatidylethanolamine flippase activity [PMID:16452632]
EXISTENCE:genetic interaction:GO:0140345 phosphatidylcholine flippase activity [PMID:16452632]
EXISTENCE:genetic interaction:GO:0140346 phosphatidylserine flippase activity [PMID:16452632]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:32661085]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:16452632|PMID:32661085]
EXISTENCE:mutant phenotype:GO:0090555 phosphatidylethanolamine flippase activity [PMID:16452632]
EXISTENCE:mutant phenotype:GO:0140345 phosphatidylcholine flippase activity [PMID:16452632]
EXISTENCE:mutant phenotype:GO:0140346 phosphatidylserine flippase activity [PMID:32661085]
Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16678774]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:16678774]
EXISTENCE:genetic interaction:GO:0045033 peroxisome inheritance [PMID:16678774]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:16678774]
EXISTENCE:mutant phenotype:GO:0045033 peroxisome inheritance [PMID:16678774]
EXISTENCE:physical interaction:GO:0043495 protein-membrane adaptor activity [PMID:16678774]
EXISTENCE:physical interaction:GO:0045033 peroxisome inheritance [PMID:16678774]
Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10515942]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10515942|PMID:9987114]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15466424]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15466424|PMID:16568252|PMID:9342405|PMID:9987114]
Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1
EXISTENCE:direct assay:GO:0000976 transcription cis-regulatory region binding [PMID:15889145]
EXISTENCE:direct assay:GO:0005773 vacuole [PMID:22121197]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:26275961]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16467296|PMID:26928762]
EXISTENCE:direct assay:GO:0008195 phosphatidate phosphatase activity [PMID:16467296|PMID:35026226]
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:20876142|PMID:35026226]
EXISTENCE:genetic interaction:GO:0019432 triglyceride biosynthetic process [PMID:24196957]
EXISTENCE:genetic interaction:GO:0034389 lipid droplet organization [PMID:21829381]
EXISTENCE:mutant phenotype:GO:0006276 plasmid maintenance [PMID:8437575]
EXISTENCE:mutant phenotype:GO:0008195 phosphatidate phosphatase activity [PMID:16467296]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:16467296|PMID:17971454|PMID:35026226]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8437575]
EXISTENCE:mutant phenotype:GO:0019432 triglyceride biosynthetic process [PMID:24196957|PMID:35026226]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:21422231]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:22121197]
Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:25585246]
EXISTENCE:direct assay:GO:0015095 magnesium ion transmembrane transporter activity [PMID:25585246]
EXISTENCE:direct assay:GO:0015693 magnesium ion transport [PMID:25585246]
EXISTENCE:mutant phenotype:GO:1990616 magnesium ion export from mitochondrion [PMID:25585246]
Transporter of the mitochondrial inner membrane that exports magnesium; involved in mitochondrial Mg2+ homeostasis; has similarity to human mitochondrial ATP-Mg/Pi carriers
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:11717305]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:16702432|PMID:23316435]
EXISTENCE:mutant phenotype:GO:0000713 meiotic heteroduplex formation [PMID:15654114]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:10938116]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:2685551]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10570173]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:11717305]
EXISTENCE:physical interaction:GO:0032389 MutLalpha complex [PMID:11237696|PMID:8066446|PMID:10570173]
EXISTENCE:physical interaction:GO:0032390 MutLbeta complex [PMID:10570173]
EXISTENCE:physical interaction:GO:0097587 MutLgamma complex [PMID:9770499|PMID:10570173]
Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; required for silencing at the silent mating-type loci and telomeres; human homolog is associated with hereditary non-polyposis colon cancer
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:10352012]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:10352012]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:11290706]
Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:10407263]
EXISTENCE:mutant phenotype:GO:0006598 polyamine catabolic process [PMID:12586697]
EXISTENCE:mutant phenotype:GO:0019483 beta-alanine biosynthetic process [PMID:12586697]
Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
EXISTENCE:mutant phenotype:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:12586697]
Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18367543|PMID:14562095]
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:18367543|PMID:30361468]
EXISTENCE:mutant phenotype:GO:1990756 ubiquitin ligase-substrate adaptor activity [PMID:30361468]
Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:11336700]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10409737]
EXISTENCE:direct assay:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:12743037]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12743037]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10722658|PMID:10409737]
EXISTENCE:mutant phenotype:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:12743037]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10409737|PMID:10722658|PMID:25904326]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12743037]
Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8444852]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:2111448]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:8444852]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:2111448]
DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004866 endopeptidase inhibitor activity [PMID:17145748]
EXISTENCE:mutant phenotype:GO:0004866 endopeptidase inhibitor activity [PMID:2037077]
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:2037077]
Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:12082171]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22181064]
EXISTENCE:mutant phenotype:GO:0042631 cellular response to water deprivation [PMID:21420397]
Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication
ARS1323; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922|PMID:14562095]
EXISTENCE:direct assay:GO:0070211 Snt2C complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:23878396]
Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9353309|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:9353309]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9353309]
Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0047884 FAD diphosphatase activity [PMID:29902190]
EXISTENCE:genetic interaction:GO:0042726 flavin-containing compound metabolic process [PMID:29902190]
FAD pyrophosphatase; hydrolyzes FAD as the preferred substrate but also acts on NADH and ADP-ribose at lower rates; activity is dependent upon K+ and divalent metal cations; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; FPY1 is not an essential gene; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21057056]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:21057056]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:8035801]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:21057056]
Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; binds with Spt10p to histone gene promoters during S phase; localizes to nuclear foci that become diffuse upon DNA replication stress
EXISTENCE:direct assay:GO:0004651 polynucleotide 5'-phosphatase activity [PMID:10219091]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10219091]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10219091]
RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; CTL1 has a paralog, CET1, that arose from the whole genome duplication
hypothetical protein; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:19372386|PMID:21206489]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16880382|PMID:1923755]
Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:31804585]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at positions U1290 and U1415
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:19502581]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17507646|PMID:19502581|PMID:16622836]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:19502581]
EXISTENCE:genetic interaction:GO:0031321 ascospore-type prospore assembly [PMID:19502581]
EXISTENCE:physical interaction:GO:0005484 SNAP receptor activity [PMID:7954793]
Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:12711700]
hypothetical protein; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23438601]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:23438601]
Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18492664]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9465043]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:18268103]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:17954556]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:7791797]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:2674684]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:12853471]
Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
ARS1324; Autonomously Replicating Sequence
hypothetical protein; YMR187C is not an essential gene
mitochondrial 37S ribosomal protein MRPS17
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24390141|PMID:24769239]
EXISTENCE:direct assay:GO:0004375 glycine dehydrogenase (decarboxylating) activity [PMID:320197|PMID:8830251]
EXISTENCE:genetic interaction:GO:0006730 one-carbon metabolic process [PMID:10871621]
EXISTENCE:mutant phenotype:GO:0019464 glycine decarboxylation via glycine cleavage system [PMID:8830251]
P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9545297]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9271578]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:10366502]
EXISTENCE:direct assay:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15899853|PMID:25588990]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:18440969]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:9545297]
EXISTENCE:direct assay:GO:0044806 G-quadruplex DNA unwinding [PMID:10198430]
EXISTENCE:genetic interaction:GO:0000706 meiotic DNA double-strand break processing [PMID:20150422]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:17550308|PMID:16546998]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:19595717]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:19158388]
EXISTENCE:genetic interaction:GO:0000729 DNA double-strand break processing [PMID:18806779]
EXISTENCE:genetic interaction:GO:0007534 gene conversion at mating-type locus [PMID:18806779]
EXISTENCE:genetic interaction:GO:0010520 regulation of reciprocal meiotic recombination [PMID:18691965]
EXISTENCE:genetic interaction:GO:0010947 negative regulation of meiotic joint molecule formation [PMID:18691964]
EXISTENCE:genetic interaction:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:19158388]
EXISTENCE:genetic interaction:GO:0031860 telomeric 3' overhang formation [PMID:19595717|PMID:20808892]
EXISTENCE:genetic interaction:GO:0044818 mitotic G2/M transition checkpoint [PMID:18805091]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:7736577]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:15994923|PMID:17550308]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:19158388]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:18805091]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:19158388]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:7736577]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:11138010]
EXISTENCE:physical interaction:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15889139]
RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:22904326]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:22904326]
Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15331637]
EXISTENCE:direct assay:GO:0005798 Golgi-associated vesicle [PMID:15331637]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15331637]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:15331637|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15331637|PMID:14562095]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:15802519]
EXISTENCE:genetic interaction:GO:0017157 regulation of exocytosis [PMID:15331637]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10992286|PMID:12857883|PMID:15802519]
EXISTENCE:physical interaction:GO:0017157 regulation of exocytosis [PMID:15331637]
Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication
ARS1325; Autonomously Replicating Sequence
mitochondrial 54S ribosomal protein YmL24/YmL14
Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
EXISTENCE:physical interaction:GO:0005730 nucleolus [PMID:2121740]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2416
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19703468]
Protein that localizes to the mitochondrial intermembrane space; localizes via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
hypothetical protein; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ATG41, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:9398683]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:19805279]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11739407]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:9398683]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:9199167]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11694599]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10385523]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:10397773]
Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways; human homolog VTI1A can complement yeast null mutant
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:11729143|PMID:1644287]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:11729143]
EXISTENCE:direct assay:GO:0005871 kinesin complex [PMID:10087265]
EXISTENCE:direct assay:GO:0008569 minus-end-directed microtubule motor activity [PMID:16107877]
EXISTENCE:direct assay:GO:0051321 meiotic cell cycle [PMID:11729143]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:1644287]
EXISTENCE:mutant phenotype:GO:0000743 nuclear migration involved in conjugation with cellular fusion [PMID:19386762]
EXISTENCE:mutant phenotype:GO:0003777 microtubule motor activity [PMID:10087265]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:11729143]
EXISTENCE:mutant phenotype:GO:0060236 regulation of mitotic spindle organization [PMID:25313961]
Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:10409718|PMID:8387915]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11080155]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:10409718|PMID:8387915]
EXISTENCE:genetic interaction:GO:0007089 traversing start control point of mitotic cell cycle [PMID:2147225|PMID:2574633]
G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16567426]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:18508919]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:16874095]
EXISTENCE:genetic interaction:GO:0006487 protein N-linked glycosylation [PMID:22492205]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:17606994]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:16874095]
EXISTENCE:genetic interaction:GO:0035269 protein O-linked mannosylation [PMID:22492205]
EXISTENCE:mutant phenotype:GO:0006458 'de novo' protein folding [PMID:18508919]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:17606994]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:9545237]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:17606994]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:18508919]
EXISTENCE:physical interaction:GO:0006458 'de novo' protein folding [PMID:18508919]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:22492205]
Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface
EXISTENCE:direct assay:GO:0000715 nucleotide-excision repair, DNA damage recognition [PMID:8516285]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:8516285|PMID:23925126]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:8516285]
Subunit of Nucleotide Excision Repair Factor 1 (NEF1); recognizes and binds damaged DNA during nucleotide excision repair (NER); also involved in regulation of transcription; contains zinc finger motif; homolog of human XPA protein
RNA of unknown function transcribed by RNA polymerase III; conserved in other yeast species
ETC5; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; located within ncRNA RNA170 which lies between divergently transcribed genes RAD14 and ERG2
EXISTENCE:curator inference:GO:0005783 endoplasmic reticulum [PMID:18459942]
EXISTENCE:mutant phenotype:GO:0000247 C-8 sterol isomerase activity [PMID:18459942]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:18459942]
C-8 sterol isomerase; catalyzes isomerization of delta-8 double bond to delta-7 position at an intermediate step in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutation is functionally complemented by human EBP
EXISTENCE:direct assay:GO:0005742 mitochondrial outer membrane translocase complex [PMID:9774667]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9774109]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:15590639]
Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore; pre-Tom40p is phosphorylated by PKA, which impairs its import into mitochondria under non-respiratory conditions
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:23900285|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:23900285]
EXISTENCE:mutant phenotype:GO:0045033 peroxisome inheritance [PMID:15928207]
Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane; Inp1p and Pex3p tether peroxisomes to plasma membrane
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558]
EXISTENCE:direct assay:GO:0005945 6-phosphofructokinase complex [PMID:4271029|PMID:6231961|PMID:21241708]
EXISTENCE:direct assay:GO:0006096 glycolytic process [PMID:3000145]
EXISTENCE:genetic interaction:GO:0003872 6-phosphofructokinase activity [PMID:3007939]
EXISTENCE:genetic interaction:GO:0006096 glycolytic process [PMID:2965996]
EXISTENCE:mutant phenotype:GO:0003872 6-phosphofructokinase activity [PMID:3007939]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:2965996|PMID:27226568]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:24860096]
EXISTENCE:mutant phenotype:GO:0051453 regulation of intracellular pH [PMID:24860096]
EXISTENCE:mutant phenotype:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:24860096]
Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
hypothetical protein; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0003989 acetyl-CoA carboxylase activity [PMID:14761959]
EXISTENCE:genetic interaction:GO:0042759 long-chain fatty acid biosynthetic process [PMID:14761959]
Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; genetic and comparative analysis suggests that translation begins at a non-canonical (Ile) start codon at -372 relative to the annotated start codon
EXISTENCE:direct assay:GO:0004496 mevalonate kinase activity [PMID:16477420|PMID:1645230]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:1645230]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:200835]
EXISTENCE:mutant phenotype:GO:0010142 farnesyl diphosphate biosynthetic process, mevalonate pathway [PMID:200835]
EXISTENCE:mutant phenotype:GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway [PMID:200835]
Mevalonate kinase; acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate; human MVK functionally complements the lethality of the erg12 null mutation
Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:29225428]
EXISTENCE:direct assay:GO:0047372 acylglycerol lipase activity [PMID:26991558]
EXISTENCE:genetic interaction:GO:0006641 triglyceride metabolic process [PMID:26991558]
EXISTENCE:genetic interaction:GO:0047372 acylglycerol lipase activity [PMID:26991558]
EXISTENCE:genetic interaction:GO:0051792 medium-chain fatty acid biosynthetic process [PMID:16361250]
EXISTENCE:mutant phenotype:GO:0047372 acylglycerol lipase activity [PMID:26991558]
Monoacylglycerol and triacylglycerol lipase; MAG lipase with preference for palmitoyl-MAG; TAG lipase involved in TAG catabolism; minor role in medium-chain fatty acid ethyl ester biosynthesis; contains an alpha/beta hydrolase domain and a typical lipase motif (GXSXG); similar to acyltransferases, Eeb1p, Eht1p, and human ABHD1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14690591]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:12702300]
EXISTENCE:mutant phenotype:GO:0006276 plasmid maintenance [PMID:12702300]
Essential protein involved in mtDNA inheritance; may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19075114]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:19075114]
Protein required for Stt4-containing PI kinase complex localization; required for Stt4-containing phosphoinositide (PI) kinase patch assembly at plasma membrane; recruited to plasma membrane via conserved basic patch near N-terminus; exhibits synthetic lethal genetic interactions with PHO85; mutations in human homolog EFR3A implicated in autism spectrum disorders
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:10092627]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:10092627]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:10092627]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:22408182]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:22408182]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10092627]
Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:7744969]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:9817751]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11381090]
EXISTENCE:mutant phenotype:GO:0034975 protein folding in endoplasmic reticulum [PMID:9817751]
EXISTENCE:mutant phenotype:GO:0051087 chaperone binding [PMID:9817751]
One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17507646|PMID:16622836]
Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11233987|PMID:17517895]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10318902]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:31399582]
EXISTENCE:direct assay:GO:0035617 stress granule disassembly [PMID:31399582]
EXISTENCE:genetic interaction:GO:0006376 mRNA splice site selection [PMID:11565750]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:11113192]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:11585720]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:12225850]
EXISTENCE:mutant phenotype:GO:0042307 positive regulation of protein import into nucleus [PMID:10952997]
SR protein kinase (SRPK); involved in mRNA 3' splice site recognition with Prp8p and Cdc40p; phosphorylates one or more SR dipeptides on mRNA export factors Npl3p and Gbp2p to stimulate cytoplasmic release of mRNA and Mtr10p-mediated nuclear reimport; component of stress granules that regulates granule disassembly during recovery from stress; modulates cation uptake and homeostasis through membrane transporters; contains prion-like domains; similar to human SRPK1
EXISTENCE:mutant phenotype:GO:0003922 GMP synthase (glutamine-hydrolyzing) activity [PMID:8112582]
EXISTENCE:mutant phenotype:GO:0006177 GMP biosynthetic process [PMID:20980241]
GMP synthase; highly conserved enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation; reduction-of-function mutation gua1-G388D causes changes in cellular guanine nucleotide pools, defects in general protein synthesis, and impaired translation of GCN4 mRNA
transport protein particle complex II subunit TRS130
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:16314430]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16314430]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17041589]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17041589]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11239471|PMID:16314430]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:23078654]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:23078654]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:16314430]
EXISTENCE:mutant phenotype:GO:0043087 regulation of GTPase activity [PMID:17041589]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17041589]
Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:27122604]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:12417731]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:12417731]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:27122604]
Protein involved in telomeric silencing; required for quiescent cell telomere hypercluster localization at nuclear membrane vicinity; interacts with PAD4-domain of Sir4p
EXISTENCE:direct assay:GO:0004631 phosphomevalonate kinase activity [PMID:1846667]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:1846667]
EXISTENCE:mutant phenotype:GO:0004631 phosphomevalonate kinase activity [PMID:1846667]
EXISTENCE:mutant phenotype:GO:0031388 organic acid phosphorylation [PMID:200835]
Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy; targeted to vacuole via AP-3 pathway
Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:14563679|PMID:12052880]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:14563679]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:20434206]
EXISTENCE:direct assay:GO:0071819 DUBm complex [PMID:20395473]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:14563679]
EXISTENCE:mutant phenotype:GO:0008380 RNA splicing [PMID:23209445]
EXISTENCE:mutant phenotype:GO:0016578 histone deubiquitination [PMID:14563679]
EXISTENCE:mutant phenotype:GO:0034729 histone H3-K79 methylation [PMID:16855026]
EXISTENCE:mutant phenotype:GO:0051568 histone H3-K4 methylation [PMID:16855026]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:20434206]
Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B
EXISTENCE:direct assay:GO:0003691 double-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0004017 adenylate kinase activity [PMID:17349953]
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:9858579]
EXISTENCE:direct assay:GO:0004520 endodeoxyribonuclease activity [PMID:16116037]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15548595|PMID:22842922|PMID:9845372]
EXISTENCE:direct assay:GO:0008408 3'-5' exonuclease activity [PMID:11454871]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:15721260|PMID:16116037]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:17698079]
EXISTENCE:genetic interaction:GO:0000727 double-strand break repair via break-induced replication [PMID:17321803]
EXISTENCE:genetic interaction:GO:0060090 molecular adaptor activity [PMID:9845372]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:17321803]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:7789757]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:20040573]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:12399380|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0010791 DNA double-strand break processing involved in repair via synthesis-dependent strand annealing [PMID:23254329]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:17396017]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:27173583]
EXISTENCE:mutant phenotype:GO:0051037 regulation of transcription involved in meiotic cell cycle [PMID:17396017]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:9845372]
EXISTENCE:mutant phenotype:GO:0097552 mitochondrial double-strand break repair via homologous recombination [PMID:22214610]
EXISTENCE:physical interaction:GO:0030870 Mre11 complex [PMID:9845372]
Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
mitochondrial 54S ribosomal protein YmL44
Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004090 carbonyl reductase (NADPH) activity [PMID:18630881]
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:17962934]
EXISTENCE:direct assay:GO:0031132 serine 3-dehydrogenase activity [PMID:12535615]
NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; catalyzes the reduction of (S)-alpha-acetolactate to 2,3-dimethylglycerate; short-chain dehydrogenase/reductase (SDR) family member; GFP fusion protein localizes to the cytoplasm and nucleus
TATA-binding protein-associated factor TAF7
TFIID subunit (67 kDa); involved in RNA polymerase II transcription initiation
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:12270918|PMID:22984289]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:12270918]
EXISTENCE:direct assay:GO:0006391 transcription initiation at mitochondrial promoter [PMID:19920143]
EXISTENCE:direct assay:GO:0034245 mitochondrial DNA-directed RNA polymerase complex [PMID:7929382]
EXISTENCE:direct assay:GO:0034246 mitochondrial transcription factor activity [PMID:20008320|PMID:7929382]
EXISTENCE:mutant phenotype:GO:0032786 positive regulation of DNA-templated transcription, elongation [PMID:31980825]
EXISTENCE:physical interaction:GO:0034246 mitochondrial transcription factor activity [PMID:7929382]
Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922|PMID:8896463]
EXISTENCE:direct assay:GO:0008266 poly(U) RNA binding [PMID:16428605]
EXISTENCE:direct assay:GO:0030490 maturation of SSU-rRNA [PMID:23630256]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:24239293]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:24239293]
EXISTENCE:direct assay:GO:0034512 box C/D RNA binding [PMID:24239293]
EXISTENCE:direct assay:GO:0034513 box H/ACA snoRNA binding [PMID:24239293]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:24239293]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8896463]
EXISTENCE:mutant phenotype:GO:0000464 endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8896463]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8896463]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8896463]
EXISTENCE:mutant phenotype:GO:0034463 90S preribosome assembly [PMID:24239293]
RNA binding protein involved in synthesis of 18S and 5.8S rRNAs; component of ribosomal small subunit (SSU) processome and 90S preribosome; required for pre-rRNA packaging and compaction of processome into dense terminal balls; part of Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription with role in biogenesis of large ribosomal subunit; binds single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication; mutations in the human homolog associated with Diamond-Blackfan anemia
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10944212]
EXISTENCE:direct assay:GO:0016574 histone ubiquitination [PMID:22570702]
EXISTENCE:direct assay:GO:0033263 CORVET complex [PMID:17488625]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:16601699]
EXISTENCE:direct assay:GO:0035542 regulation of SNARE complex assembly [PMID:18385512]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:22570702]
EXISTENCE:direct assay:GO:0099022 vesicle tethering [PMID:27307091]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:11422941]
EXISTENCE:genetic interaction:GO:0045324 late endosome to vacuole transport [PMID:11422941]
EXISTENCE:mutant phenotype:GO:0036205 histone catabolic process [PMID:22570702]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:physical interaction:GO:0030897 HOPS complex [PMID:10944212]
Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18474625|PMID:26928762]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18474625]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:7559752]
EXISTENCE:genetic interaction:GO:0000755 cytogamy [PMID:3302672]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:7559752]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:22323294]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:3302672|PMID:7559752]
EXISTENCE:mutant phenotype:GO:0031385 regulation of termination of mating projection growth [PMID:14734532]
Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p
Non-essential sumoylated hypothetical protein; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:7489497]
EXISTENCE:direct assay:GO:0006401 RNA catabolic process [PMID:7489497]
Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:9305944|PMID:7657689|PMID:7744835]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8755533]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:2116418|PMID:8755533]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:7657689]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:9305944]
EXISTENCE:direct assay:GO:0090630 activation of GTPase activity [PMID:7657689|PMID:9305944|PMID:7744835]
EXISTENCE:genetic interaction:GO:0006409 tRNA export from nucleus [PMID:22008473]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:7657689]
EXISTENCE:mutant phenotype:GO:0006404 RNA import into nucleus [PMID:16040803]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:22008473]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:17904525]
EXISTENCE:mutant phenotype:GO:0090630 activation of GTPase activity [PMID:7657689]
GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:10747053]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:9844639]
Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
EXISTENCE:direct assay:GO:0034044 exomer complex [PMID:17000877]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:16498409]
EXISTENCE:physical interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:16498409]
Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
putative mannan endo-1,6-alpha-mannosidase
EXISTENCE:direct assay:GO:0009272 fungal-type cell wall biogenesis [PMID:12421307]
EXISTENCE:genetic interaction:GO:0007117 budding cell bud growth [PMID:15470258]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:17158880]
EXISTENCE:direct assay:GO:0004525 ribonuclease III activity [PMID:15766245]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15090619]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15090619]
EXISTENCE:direct assay:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:19818713]
EXISTENCE:direct assay:GO:0034476 U5 snRNA 3'-end processing [PMID:9334335]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:22576366]
EXISTENCE:mutant phenotype:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:17158880]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:17991894]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:17991894]
EXISTENCE:mutant phenotype:GO:0009303 rRNA transcription [PMID:17991894]
EXISTENCE:mutant phenotype:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:19818713]
EXISTENCE:mutant phenotype:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172|PMID:9334335]
EXISTENCE:mutant phenotype:GO:0034963 box C/D RNA processing [PMID:23201338]
EXISTENCE:mutant phenotype:GO:0034964 box H/ACA RNA processing [PMID:23201338]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:22576366]
Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:8566755]
Protein required for assembly of U2 snRNP into the spliceosome; forms a complex with Hsh49p and Hsh155p
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:9742088]
EXISTENCE:direct assay:GO:0005371 tricarboxylate secondary active transmembrane transporter activity [PMID:20371607|PMID:9559855]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:20371607|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006842 tricarboxylic acid transport [PMID:9559855]
EXISTENCE:direct assay:GO:0006843 mitochondrial citrate transmembrane transport [PMID:20371607]
EXISTENCE:direct assay:GO:0015742 alpha-ketoglutarate transport [PMID:20371607]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:9742088]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:9742088]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:9742088]
Citrate and oxoglutarate carrier protein; exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:23874617]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:23874617]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:23874617]
Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:9712830]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23836928|PMID:26928762]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:mutant phenotype:GO:0005385 zinc ion transmembrane transporter activity [PMID:2693940]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:10856230]
Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
hypothetical protein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
ARS1327; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:25959673]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:24549041]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:24549041|PMID:22984289]
Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:7650027]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093|PMID:34161133]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27136724]
EXISTENCE:genetic interaction:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:11477098|PMID:22633490]
EXISTENCE:genetic interaction:GO:0035336 long-chain fatty-acyl-CoA metabolic process [PMID:11477098]
EXISTENCE:genetic interaction:GO:0044539 long-chain fatty acid import into cell [PMID:12601005|PMID:27136724]
EXISTENCE:genetic interaction:GO:1905329 sphingoid long-chain base transport [PMID:27136724]
EXISTENCE:mutant phenotype:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:11477098]
EXISTENCE:mutant phenotype:GO:0035336 long-chain fatty-acyl-CoA metabolic process [PMID:11477098]
Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2314
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:17283062]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17283062]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:17283062]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:25554787]
EXISTENCE:direct assay:GO:1990112 RQC complex [PMID:23178123]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:17283062]
EXISTENCE:genetic interaction:GO:0010498 proteasomal protein catabolic process [PMID:24261871]
EXISTENCE:genetic interaction:GO:0030163 protein catabolic process [PMID:27481473]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:20835226]
EXISTENCE:mutant phenotype:GO:0010498 proteasomal protein catabolic process [PMID:24261871]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:20835226]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:27481473]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:17283062]
EXISTENCE:mutant phenotype:GO:0072344 rescue of stalled ribosome [PMID:26943317]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23358411|PMID:23479637]
EXISTENCE:physical interaction:GO:1990112 RQC complex [PMID:23479637]
RING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins and translationally stalled ER membrane proteins; component of ribosome-bound RQC (ribosome quality control) complex; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration
EXISTENCE:mutant phenotype:GO:0004351 glutamate decarboxylase activity [PMID:11031268]
EXISTENCE:mutant phenotype:GO:0006538 glutamate catabolic process [PMID:11031268]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11031268]
Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
ARS1328; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:16709151]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16936141]
Omega class glutathione transferase; putative cytosolic localization
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15208314]
EXISTENCE:mutant phenotype:GO:0007009 plasma membrane organization [PMID:15208314]
hypothetical protein; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
Mitochondrial hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; MLO1 is not an essential gene
Putative membrane protein, involved in salt tolerance; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10523319]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:10610322]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:10523319|PMID:10610322]
Coiled-coiled hypothetical protein; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
Subunit VII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11106667]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:3034718]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:3034718]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:3034718]
Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
EXISTENCE:direct assay:GO:0005095 GTPase inhibitor activity [PMID:21389113]
EXISTENCE:direct assay:GO:0034260 negative regulation of GTPase activity [PMID:21389113]
EXISTENCE:genetic interaction:GO:0006623 protein targeting to vacuole [PMID:21389113]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:21389113]
GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
EXISTENCE:mutant phenotype:GO:0002130 wobble position ribose methylation [PMID:22912484]
EXISTENCE:physical interaction:GO:0002130 wobble position ribose methylation [PMID:22912484]
Protein involved in 2'-O-methylation of C32 of substrate tRNAs; interacts with 2'-O-ribose methyltransferase Trm7p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene; yeast null mutant can be functionally complemented by human THADA, mutations in which have been implicated in epithelial thyroid adenomas, type 2 diabetes, and polycystic ovary syndrome (PCOS)
EXISTENCE:direct assay:GO:0001731 formation of translation preinitiation complex [PMID:12008673|PMID:15664195|PMID:17434125]
EXISTENCE:direct assay:GO:0003725 double-stranded RNA binding [PMID:17673174]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:12008673]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:16380131]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:10982835|PMID:16380131]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0043024 ribosomal small subunit binding [PMID:17434125|PMID:12860115|PMID:16461768]
EXISTENCE:genetic interaction:GO:0001732 formation of cytoplasmic translation initiation complex [PMID:17242201]
EXISTENCE:mutant phenotype:GO:0001677 formation of translation initiation ternary complex [PMID:20048003]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:12514125|PMID:15838098|PMID:16193068|PMID:17332751]
EXISTENCE:mutant phenotype:GO:0001732 formation of cytoplasmic translation initiation complex [PMID:16461768|PMID:20048003]
EXISTENCE:mutant phenotype:GO:0002188 translation reinitiation [PMID:16193068|PMID:20048003]
EXISTENCE:mutant phenotype:GO:0043024 ribosomal small subunit binding [PMID:16193068]
Translation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and 40S ribosomal subunit and scans for start codons; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3; ortholog of bacterial translation initiation factor IF1
EXISTENCE:mutant phenotype:GO:0030234 enzyme regulator activity [PMID:9837904]
Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
hypothetical protein; interacts weakly with Knr4p; YMR262W is not an essential gene
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:9651585]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19270272]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17210643|PMID:20398213]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:10388812|PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:10388812|PMID:16286008|PMID:10082585]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:20398213]
EXISTENCE:mutant phenotype:GO:2000219 positive regulation of invasive growth in response to glucose limitation [PMID:18202364]
Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:23665229]
EXISTENCE:direct assay:GO:0097027 ubiquitin-protein transferase activator activity [PMID:18321851]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:9388185]
EXISTENCE:mutant phenotype:GO:0097051 establishment of protein localization to endoplasmic reticulum membrane [PMID:9388185]
Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:17005914]
Membrane hypothetical protein; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:14576278|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0004427 inorganic diphosphate phosphatase activity [PMID:1648084]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:1648084]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:1648084]
Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate; human ortholog, PPA2, functionally complements the null mutant; mutations in human PPA2 cause a mitochondrial disease resulting in sudden unexpected cardiac arrest in infants
EXISTENCE:direct assay:GO:0000244 spliceosomal tri-snRNP complex assembly [PMID:9452384]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:20181740]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:10022888]
EXISTENCE:physical interaction:GO:0017069 snRNA binding [PMID:16410014]
Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:17425675|PMID:14562095]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:17425675]
Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
ARS1329; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000500 RNA polymerase I upstream activating factor complex [PMID:8846924]
EXISTENCE:direct assay:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:8846924]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:9632758]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
EXISTENCE:mutant phenotype:GO:0017025 TBP-class protein binding [PMID:9632758]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:9632758]
Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
EXISTENCE:genetic interaction:GO:0004588 orotate phosphoribosyltransferase activity [PMID:2182197]
EXISTENCE:genetic interaction:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:2182197]
EXISTENCE:genetic interaction:GO:0046132 pyrimidine ribonucleoside biosynthetic process [PMID:2182197]
EXISTENCE:mutant phenotype:GO:0004588 orotate phosphoribosyltransferase activity [PMID:2182197]
EXISTENCE:mutant phenotype:GO:0046132 pyrimidine ribonucleoside biosynthetic process [PMID:2182197]
Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
EXISTENCE:genetic interaction:GO:0070125 mitochondrial translational elongation [PMID:15706032]
EXISTENCE:mutant phenotype:GO:1900180 regulation of protein localization to nucleus [PMID:25527290]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; can mutate to cause constitutive pseudohyphal growth in homozygous diploids; required for localization of transcription factor Gln3p to the nucleus in response to nitrogen starvation or rapamycin treatment
EXISTENCE:genetic interaction:GO:0006673 inositol phosphoceramide metabolic process [PMID:9559540]
EXISTENCE:mutant phenotype:GO:0000038 very long-chain fatty acid metabolic process [PMID:9353282]
EXISTENCE:mutant phenotype:GO:0000170 sphingosine hydroxylase activity [PMID:9353282]
EXISTENCE:mutant phenotype:GO:0006673 inositol phosphoceramide metabolic process [PMID:16652392|PMID:9353282|PMID:9368039|PMID:9559540]
EXISTENCE:mutant phenotype:GO:0080132 fatty acid alpha-hydroxylase activity [PMID:9353282|PMID:9559540]
Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8816439]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:8816439]
EXISTENCE:genetic interaction:GO:0004864 protein phosphatase inhibitor activity [PMID:18762578]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:15619606]
EXISTENCE:mutant phenotype:GO:0004864 protein phosphatase inhibitor activity [PMID:18762578]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:15619606]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8816439]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:10662670]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:18762578]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:15619606]
Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:17942791]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9736709]
EXISTENCE:genetic interaction:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:10825201|PMID:11581258|PMID:9065405]
EXISTENCE:genetic interaction:GO:0071586 CAAX-box protein processing [PMID:9065405]
EXISTENCE:mutant phenotype:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:10825201]
EXISTENCE:mutant phenotype:GO:0071586 CAAX-box protein processing [PMID:16361710|PMID:9065405]
Type II CAAX prenyl protease; involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
ARS1330; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000151 ubiquitin ligase complex [PMID:9931424]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:10366593]
EXISTENCE:genetic interaction:GO:0070086 ubiquitin-dependent endocytosis [PMID:29514933]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:8668140]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:8668140]
Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex; ubiquitin-binding adaptor involved in down-regulation of general amino acid transporter Gap1p upon shift to preferred nitrogen source; involved in lactate transporter Jen1p endocytosis; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
ubiquitin domain-containing protein DSK2
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:8070654]
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:8070654]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:15167887]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:11805328]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:29444958]
Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; K48-Ub chain binding protein that also binds K63-linked chains in vitro; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; contains an N-terminal Ub-like (UBL) and a C-terminal Ub-associated (UBA) domain; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:10445027|PMID:11904169]
EXISTENCE:direct assay:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:10445027|PMID:11904169|PMID:16301539]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10445027|PMID:12370301]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:10445027]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10445027]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:31506296]
EXISTENCE:mutant phenotype:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:10445027]
Carboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0008973 phosphopentomutase activity [PMID:23103740]
EXISTENCE:mutant phenotype:GO:0006148 inosine catabolic process [PMID:23670538]
EXISTENCE:mutant phenotype:GO:0006166 purine ribonucleoside salvage [PMID:23670538]
EXISTENCE:mutant phenotype:GO:0008973 phosphopentomutase activity [PMID:23103740|PMID:23670538]
EXISTENCE:mutant phenotype:GO:0046115 guanosine catabolic process [PMID:23670538]
Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
EXISTENCE:mutant phenotype:GO:0035445 borate transmembrane transport [PMID:21621519]
Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10540293|PMID:11405098|PMID:14685767]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:14685767]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11024040|PMID:7891685|PMID:8710504]
EXISTENCE:mutant phenotype:GO:0061414 positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:10540293|PMID:10628872|PMID:11024040|PMID:7891685|PM ID:8710504|PMID:9894915]
Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
N-acetylglucosaminylphosphatidylinositol deacetylase
EXISTENCE:direct assay:GO:0000225 N-acetylglucosaminylphosphatidylinositol deacetylase activity [PMID:10085243]
ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7635208|PMID:24769239|PMID:14576278|PMID:16823961]
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:10101162]
Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
tRNA A64-2'-O-ribosylphosphate transferase
EXISTENCE:direct assay:GO:0016763 pentosyltransferase activity [PMID:7954819]
EXISTENCE:mutant phenotype:GO:0016763 pentosyltransferase activity [PMID:7954819]
EXISTENCE:mutant phenotype:GO:0019988 charged-tRNA amino acid modification [PMID:7954819]
Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
ARS1331; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10638763]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:10818099|PMID:8604297]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:18791224]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:10818099|PMID:8604297|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:10908335|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:18791224]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:9501103|PMID:26587833]
Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:12358428]
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:12358428]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12358428]
Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
mitochondrial 54S ribosomal protein YmL33
EXISTENCE:direct assay:GO:0008859 exoribonuclease II activity [PMID:17658549|PMID:12426313]
EXISTENCE:direct assay:GO:0045025 mitochondrial degradosome [PMID:12426313]
EXISTENCE:mutant phenotype:GO:0000957 mitochondrial RNA catabolic process [PMID:12426313|PMID:9829834]
3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:12773561]
EXISTENCE:physical interaction:GO:0000245 spliceosomal complex assembly [PMID:10688664]
U2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
EXISTENCE:direct assay:GO:0008696 4-amino-4-deoxychorismate lyase activity [PMID:17873082]
EXISTENCE:mutant phenotype:GO:0046656 folic acid biosynthetic process [PMID:17873082]
Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase); catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15718299]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:15718299]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10684247]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15049817|PMID:10684247]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:15718299]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15049817]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23788678]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:15242642]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:15049817|PMID:23788678]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:15049817|PMID:23788678]
EXISTENCE:mutant phenotype:GO:1990417 snoRNA release from pre-rRNA [PMID:16908538]
ATP-dependent RNA helicase; involved in the biogenesis of 40S and 60S ribosome subunits; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22289182|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:14562095]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:25593311]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:22289182|PMID:25593311]
Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:10406798]
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10406798]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:19383723]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:19383723]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:19383723]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:10406798]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:19383723|PMID:10406798]
Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
glutamyl-tRNA(Gln) amidotransferase subunit HER2
EXISTENCE:direct assay:GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex [PMID:19417106]
EXISTENCE:direct assay:GO:0050567 glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity [PMID:19417106]
EXISTENCE:direct assay:GO:0070681 glutaminyl-tRNAGln biosynthesis via transamidation [PMID:19417106]
EXISTENCE:genetic interaction:GO:0007029 endoplasmic reticulum organization [PMID:18667535]
Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:9658168]
EXISTENCE:direct assay:GO:0005869 dynactin complex [PMID:9658168]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:8138567]
EXISTENCE:mutant phenotype:GO:0007097 nuclear migration [PMID:8138567]
Component of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
hypothetical protein that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:1556076]
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:mutant phenotype:GO:0004758 serine C-palmitoyltransferase activity [PMID:1556076]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:1556075]
Component of serine palmitoyltransferase; responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:14562095]
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:29514932]
EXISTENCE:direct assay:GO:0004185 serine-type carboxypeptidase activity [PMID:8679540]
EXISTENCE:direct assay:GO:0046938 phytochelatin biosynthetic process [PMID:17408619]
EXISTENCE:genetic interaction:GO:0004185 serine-type carboxypeptidase activity [PMID:29514932]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:29514932]
EXISTENCE:genetic interaction:GO:0031638 zymogen activation [PMID:29514932]
EXISTENCE:mutant phenotype:GO:0004185 serine-type carboxypeptidase activity [PMID:8679540|PMID:29514932]
EXISTENCE:mutant phenotype:GO:0046938 phytochelatin biosynthetic process [PMID:17408619]
Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; member of serine carboxypeptidase family; N-glycosylated
sphingosine N-acyltransferase subunit LIP1
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14690591|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15692566]
EXISTENCE:direct assay:GO:0061576 acyl-CoA ceramide synthase complex [PMID:15692566]
EXISTENCE:mutant phenotype:GO:0046513 ceramide biosynthetic process [PMID:15692566]
EXISTENCE:mutant phenotype:GO:0050291 sphingosine N-acyltransferase activity [PMID:15692566]
EXISTENCE:physical interaction:GO:0046513 ceramide biosynthetic process [PMID:15692566]
Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:14764870|PMID:15642746]
EXISTENCE:mutant phenotype:GO:0003774 cytoskeletal motor activity [PMID:15642746]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:14764870|PMID:15642746]
EXISTENCE:physical interaction:GO:0005868 cytoplasmic dynein complex [PMID:15642746]
Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
EXISTENCE:direct assay:GO:0004044 amidophosphoribosyltransferase activity [PMID:11689683]
EXISTENCE:mutant phenotype:GO:0004044 amidophosphoribosyltransferase activity [PMID:11689683|PMID:6376509]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:11689683]
Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase
ATP-binding cassette Fe/S cluster precursor transporter ATM1
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7828591]
EXISTENCE:direct assay:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:16754360]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10406803]
EXISTENCE:mutant phenotype:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:7828591]
EXISTENCE:mutant phenotype:GO:1902497 iron-sulfur cluster transmembrane transport [PMID:31040179]
Mitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; human homolog ABCB7 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8649384]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:8514129]
Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:3546317]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0006067 ethanol metabolic process [PMID:3546317]
Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:10527495|PMID:21665945|PMID:21710968|PMID:25877870]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:21665945]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:21665945]
EXISTENCE:direct assay:GO:0010995 free ubiquitin chain depolymerization [PMID:21665945]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:21665945|PMID:25877870]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:33226341]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:21665945]
EXISTENCE:mutant phenotype:GO:0010995 free ubiquitin chain depolymerization [PMID:21665945]
Ubiquitin-specific protease involved in protein deubiquitination; forms a complex with AAA peroxins Pex1p and Pex6p; deubiquitinates mono- and polyubiquitinated forms of Pex5p; deubiquitinates Clb5p, counteracting APC activity, and facilitating both Clb5p accumulation and S phase entry; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:19129178]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460|PMID:9748433]
EXISTENCE:genetic interaction:GO:0000747 conjugation with cellular fusion [PMID:9748433]
Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:17158736]
Protein involved in spore wall assembly; has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0000936 primary cell septum [PMID:19793924]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:19793924]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:1824714|PMID:19793924|PMID:16622836]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460|PMID:19793924]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:18462190]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:19541632]
EXISTENCE:direct assay:GO:0042124 1,3-beta-glucanosyltransferase activity [PMID:10809732|PMID:15355340|PMID:17397106]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:10716729]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:16986442]
EXISTENCE:mutant phenotype:GO:0007568 aging [PMID:31275960]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9515908]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:19541632]
EXISTENCE:mutant phenotype:GO:1905897 regulation of response to endoplasmic reticulum stress [PMID:31275960]
EXISTENCE:physical interaction:GO:0031507 heterochromatin formation [PMID:19541632]
Beta-1,3-glucanosyltransferase required for cell wall assembly; also has role in transcriptional silencing; localizes to cell surface via glycosylphosphatidylinositol (GPI) anchor, also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate functions for Gas1p in DNA damage response and cell cycle regulation; protein demonstrates amyloid properties in vivo
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9238021]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9238021]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:11694505]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:9238021]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:16651379]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:11274141]
EXISTENCE:physical interaction:GO:0061608 nuclear import signal receptor activity [PMID:11274141]
Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p; required for stability of the Dam1p-Duo1p complex
translation initiation factor eIF3 core subunit c
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1332047|PMID:11914276]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9660829|PMID:9671501]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:19470581|PMID:26777405]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:15145951]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:9722586]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:15145951]
eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection; eIF3 is also involved in programmed stop codon readthrough
EXISTENCE:curator inference:GO:0032259 methylation [PMID:21858014|PMID:22650761]
Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:12127071]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:7565759]
Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
ARS1332; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:22343726]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:22343726]
EXISTENCE:direct assay:GO:0033588 elongator holoenzyme complex [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:22343726]
EXISTENCE:mutant phenotype:GO:0032447 protein urmylation [PMID:14551258]
Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
bifunctional triglyceride lipase/lysophosphatidylethanolamine acyltransferase
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:12682047]
EXISTENCE:direct assay:GO:0071618 lysophosphatidylethanolamine acyltransferase activity [PMID:20016004]
EXISTENCE:genetic interaction:GO:0007114 cell budding [PMID:19150427]
EXISTENCE:genetic interaction:GO:0019433 triglyceride catabolic process [PMID:16267052]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:12682047]
EXISTENCE:mutant phenotype:GO:0007114 cell budding [PMID:19150427]
EXISTENCE:mutant phenotype:GO:0019433 triglyceride catabolic process [PMID:16267052]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:10515935]
Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation
proteasome core particle subunit alpha 6
Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:19712762]
EXISTENCE:genetic interaction:GO:0006740 NADPH regeneration [PMID:19712762]
Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:11063681]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:11063681]
hypothetical protein; involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W
hypothetical protein; has some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene
EXISTENCE:direct assay:GO:0006066 alcohol metabolic process [PMID:11742541]
EXISTENCE:direct assay:GO:0006081 cellular aldehyde metabolic process [PMID:11742541]
EXISTENCE:direct assay:GO:0008106 alcohol dehydrogenase (NADP+) activity [PMID:11742541]
EXISTENCE:mutant phenotype:GO:0033833 hydroxymethylfurfural reductase (NADH) activity [PMID:16652391]
EXISTENCE:mutant phenotype:GO:0033845 hydroxymethylfurfural reductase (NADPH) activity [PMID:16652391]
EXISTENCE:mutant phenotype:GO:0033859 furaldehyde metabolic process [PMID:16652391]
NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005375 copper ion transmembrane transporter activity [PMID:11023834]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:9115232]
EXISTENCE:genetic interaction:GO:0006829 zinc ion transport [PMID:12095998]
EXISTENCE:mutant phenotype:GO:0005375 copper ion transmembrane transporter activity [PMID:11023834]
EXISTENCE:mutant phenotype:GO:0005381 iron ion transmembrane transporter activity [PMID:7929320]
EXISTENCE:mutant phenotype:GO:0006829 zinc ion transport [PMID:12095998]
EXISTENCE:mutant phenotype:GO:0015677 copper ion import [PMID:11023834]
EXISTENCE:mutant phenotype:GO:0015680 protein maturation by copper ion transfer [PMID:11523804]
EXISTENCE:mutant phenotype:GO:0034755 iron ion transmembrane transport [PMID:7929320]
Low-affinity Fe(II) transporter of the plasma membrane
hypothetical protein; conserved among S. cerevisiae strains; YMR320W is not an essential gene
hypothetical protein; proposed to be a palmitoylated membrane protein; YMR321C has a paralog, SAM4, that arose from a single-locus duplication
ARS1333; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
glutathione-independent methylglyoxalase family protein
EXISTENCE:direct assay:GO:0019172 glyoxalase III activity [PMID:26370081]
EXISTENCE:mutant phenotype:GO:0019172 glyoxalase III activity [PMID:26370081]
EXISTENCE:mutant phenotype:GO:0031669 cellular response to nutrient levels [PMID:24706893]
Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
EXISTENCE:direct assay:GO:0004634 phosphopyruvate hydratase activity [PMID:23359425]
EXISTENCE:genetic interaction:GO:0004634 phosphopyruvate hydratase activity [PMID:23359425]
Enolase, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate; complements the growth defect of an ENO1 ENO2 double mutant in glucose
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions
ARS1335; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
TEL13R; Telomeric region on the right arm of Chromosome XIII; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats