Saccharomyces cerevisiae S288C chromosome XII, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome XII
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA, USA
Submitted (06-FEB-2013) Department of Genetics, Stanford University, Stanford, CA, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006945.\n\nOn Apr 26, 2011 this sequence version replaced NC_001144.4.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL12L; Telomeric region on the left arm of Chromosome XII; composed of an X element core sequence, X element combinatorial repeats, and three distinct regions of telomeric repeats separated by two short Y' elements
Putative Y' element ATP-dependent helicase
hypothetical protein; overexpression causes a cell cycle delay or arrest
Putative Y' element ATP-dependent helicase; YLL066C is not an essential gene
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau6p
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:mutant phenotype:GO:0043387 mycotoxin catabolic process [PMID:12478589]
EXISTENCE:mutant phenotype:GO:0045462 trichothecene 3-O-acetyltransferase activity [PMID:12478589]
Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis; localized to peroxisomes
S-adenosylmethionine-homocysteine S-methyltransferase MHT1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0008898 S-adenosylmethionine-homocysteine S-methyltransferase activity [PMID:11013242]
EXISTENCE:mutant phenotype:GO:0000096 sulfur amino acid metabolic process [PMID:11013242]
S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000100 S-methylmethionine transmembrane transporter activity [PMID:10497160]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:10497160]
EXISTENCE:mutant phenotype:GO:0015806 S-methylmethionine transport [PMID:10497160]
High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:9792709]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0006749 glutathione metabolic process [PMID:9792709]
Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003961 O-acetylhomoserine aminocarboxypropyltransferase activity [PMID:36379252]
EXISTENCE:mutant phenotype:GO:0006790 sulfur compound metabolic process [PMID:36379252]
Inefficient homocysteine synthase; catalyzes de novo homocysteine biosynthesis; capable of catalyzing same reaction as Met17p; affects S-methylmethionine metabolism; similar to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; HSU1 is not an essential gene
EXISTENCE:direct assay:GO:0000907 sulfonate dioxygenase activity [PMID:10482536]
EXISTENCE:mutant phenotype:GO:0044273 sulfur compound catabolic process [PMID:10482536]
Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase; involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:28265724]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:28265724]
NADH-dependent aldehyde reductase; reduces many aldehyde compounds including glycolaldehyde, formaldehyde and furfural; transcription unregulated by furfural, 5-hydroxymethylfurfural (HMF) and the mycotoxin patulin; expression increased by multidrug resistance transcription factors Yrm1p and Yrr1p; member of atypical subgroup 7 of the short-chain dehydrogenase/reductase (SDR) family; localizes to the cytoplasm; SWAT-GFP and mCherry fusion proteins localize to the ER and vacuole respectively
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0033229 cysteine transmembrane transporter activity [PMID:17435223]
EXISTENCE:direct assay:GO:0042883 cysteine transport [PMID:17435223]
EXISTENCE:mutant phenotype:GO:0033229 cysteine transmembrane transporter activity [PMID:17435223]
EXISTENCE:mutant phenotype:GO:0042883 cysteine transport [PMID:17435223]
High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
ARS1202; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
hypothetical protein with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11168368]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11168368]
EXISTENCE:direct assay:GO:0006833 water transport [PMID:11158584]
EXISTENCE:direct assay:GO:0015250 water channel activity [PMID:11158584]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:11158584]
EXISTENCE:genetic interaction:GO:0055085 transmembrane transport [PMID:11158584]
Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
EXISTENCE:direct assay:GO:0000293 ferric-chelate reductase activity [PMID:17681937]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:17553781|PMID:17681937]
EXISTENCE:genetic interaction:GO:0015677 copper ion import [PMID:17553781]
EXISTENCE:mutant phenotype:GO:0000293 ferric-chelate reductase activity [PMID:17681937]
EXISTENCE:mutant phenotype:GO:0006880 intracellular sequestering of iron ion [PMID:17681937]
Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:16569665]
EXISTENCE:direct assay:GO:0030042 actin filament depolymerization [PMID:16569665|PMID:8421056|PMID:8440472]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:8163554|PMID:8421056]
EXISTENCE:direct assay:GO:0051014 actin filament severing [PMID:14680631|PMID:8421056]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:16569665|PMID:8421056|PMID:8440472]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:22553351]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:16569665|PMID:17875745]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:22553351]
Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
EXISTENCE:direct assay:GO:0005869 dynactin complex [PMID:18245366]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:18245366]
Component of the dynactin complex; dynactin is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:9182565]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23836928]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:mutant phenotype:GO:0015718 monocarboxylic acid transport [PMID:9182565]
EXISTENCE:mutant phenotype:GO:0033285 ATPase-coupled monocarboxylic acid transmembrane transporter activity [PMID:9182565]
Transporter of ATP-binding cassette (ABC) family; targeted to vacuole via AP-3 pathway; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8341595]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:8341595]
Ribonucleoprotein that contains two RNA recognition motifs (RRM); RNP1 has a paralog, SBP1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22893726]
Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB during assembly of large subunit; binds Domain I of 25S, 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; RPL8B has paralog RPL8A from whole genome duplication; RPL8A or RPL8B ribosomes not functionally interchangeable; proportions of Rpl8ap, Rpl8bp in 80S ribosome differ in glucose and glycerol; homologous to mammalian ribosomal protein L7A, no bacterial homolog
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20026328|PMID:11914276]
EXISTENCE:direct assay:GO:0015168 glycerol transmembrane transporter activity [PMID:12486125|PMID:7729414|PMID:8550015]
EXISTENCE:direct assay:GO:0015793 glycerol transmembrane transport [PMID:12486125]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:9281576]
EXISTENCE:mutant phenotype:GO:0006846 acetate transport [PMID:17620418]
EXISTENCE:mutant phenotype:GO:0015168 glycerol transmembrane transporter activity [PMID:7729414|PMID:8550015]
EXISTENCE:mutant phenotype:GO:0015254 glycerol channel activity [PMID:22593571]
EXISTENCE:mutant phenotype:GO:0015700 arsenite transport [PMID:20026328]
EXISTENCE:mutant phenotype:GO:0015791 polyol transmembrane transport [PMID:23475614]
EXISTENCE:mutant phenotype:GO:0015793 glycerol transmembrane transport [PMID:22593571|PMID:23475614]
Aquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in transport of acetate, arsenite, and antimonite; key factor in maintaining redox balance by mediating passive diffusion of glycerol; phosphorylated by Hog1p and Slt2p MAPK; deletion improves xylose fermentation; regulated by Rgc1p and Ask10p, which are regulated by Hog1p phosphorylation under osmotic stress; phosphorylation by Ypk1p required to maintain an open state
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:10508157]
EXISTENCE:mutant phenotype:GO:0019777 Atg12 transferase activity [PMID:10508157]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10508157]
EXISTENCE:mutant phenotype:GO:0032446 protein modification by small protein conjugation [PMID:10508157|PMID:9759731]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:physical interaction:GO:0032446 protein modification by small protein conjugation [PMID:10508157]
Conserved E2-like conjugating enzyme; mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
succinate dehydrogenase iron-sulfur protein subunit SDH2
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23509072]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005749 mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) [PMID:9822678]
EXISTENCE:direct assay:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:13445213]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:1939170]
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921]
EXISTENCE:genetic interaction:GO:0045333 cellular respiration [PMID:16232921]
EXISTENCE:mutant phenotype:GO:0006099 tricarboxylic acid cycle [PMID:10224250]
EXISTENCE:mutant phenotype:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:16232921]
Iron-sulfur protein subunit of succinate dehydrogenase; the complex couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; other members are Sdh1p, Sdh3p, and Sdh4p
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:28122955]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:22442115|PMID:24390141]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:32690699]
EXISTENCE:direct assay:GO:0006895 Golgi to endosome transport [PMID:28122955]
EXISTENCE:direct assay:GO:0044232 organelle membrane contact site [PMID:27280386]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:26370498]
EXISTENCE:direct assay:GO:0090083 regulation of inclusion body assembly [PMID:31433985]
EXISTENCE:direct assay:GO:0120009 intermembrane lipid transfer [PMID:30093493]
EXISTENCE:direct assay:GO:0120014 phospholipid transfer activity [PMID:30093493]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:26370498]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:26370498]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8887651]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:32690699]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:22442115|PMID:26605945]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:9314526]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:9265642]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:32690699]
Lipid transport protein; involved in vacuolar protein sorting, protein retention in the Golgi, prospore membrane formation and sporulation; required for mitochondrial integrity and ER packaging into autophagosomes during cortical reticulophagy; peripheral membrane protein found at the prospore membrane and at membrane contact sites; contains a PH-like domain; homologous to human CHAC and COH1, involved in Chorea-acanthocytosis and Cohen syndrome, respectively
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:22375831]
EXISTENCE:direct assay:GO:0031386 protein tag [PMID:3038523]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:8035826]
Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via ubiquitin-26S proteasome system; essential for cellular stress response; encoded as polyubiquitin precursor comprised of 5 head-to-tail repeats; number of repeats is evolutionarily unstable within and between yeast species; protein abundance increases in response to DNA replication stress; implicated as modulator of replicative lifespan and meiotic recombination
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:19574226]
hypothetical protein; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:8248176]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:8194532]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:12627222]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:11914276]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:8248176]
EXISTENCE:mutant phenotype:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:12970570]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:12627222]
Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:26638174]
EXISTENCE:direct assay:GO:0051731 polynucleotide 5'-hydroxyl-kinase activity [PMID:20814424]
EXISTENCE:mutant phenotype:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23175604]
EXISTENCE:mutant phenotype:GO:0006363 termination of RNA polymerase I transcription [PMID:20814424]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12837249]
EXISTENCE:physical interaction:GO:0030874 nucleolar chromatin [PMID:20814424]
Polynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to cleave pre-rRNA at a specific site during rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11447111|PMID:14562095]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11447111|PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:18559667]
EXISTENCE:genetic interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:18559667]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11447111]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:18559667]
Putative ATPase of the AAA family; required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:18085829]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:12432101]
hypothetical protein; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
hypothetical protein; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
mannose-ethanolamine phosphotransferase GPI13
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:12441642|PMID:10793139]
EXISTENCE:mutant phenotype:GO:0016772 transferase activity, transferring phosphorus-containing groups [PMID:10793139]
EXISTENCE:mutant phenotype:GO:0051377 mannose-ethanolamine phosphotransferase activity [PMID:12441642]
ER membrane localized phosphoryltransferase; adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
hypothetical protein; conserved across S. cerevisiae strains; identified in a screen for mutants with increased levels of rDNA transcription
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18281282]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18281282]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18281282]
Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
EXISTENCE:direct assay:GO:0000296 spermine transport [PMID:15637075]
EXISTENCE:direct assay:GO:0000297 spermine transmembrane transporter activity [PMID:15637075]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12562762|PMID:15637075]
EXISTENCE:direct assay:GO:0015606 spermidine transmembrane transporter activity [PMID:15637075]
EXISTENCE:direct assay:GO:0015848 spermidine transport [PMID:15637075]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:13679573]
EXISTENCE:mutant phenotype:GO:0000296 spermine transport [PMID:11171066|PMID:15637075|PMID:9920864]
EXISTENCE:mutant phenotype:GO:0000297 spermine transmembrane transporter activity [PMID:11171066|PMID:15637075|PMID:9920864]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:15637075]
EXISTENCE:mutant phenotype:GO:0015606 spermidine transmembrane transporter activity [PMID:11171066|PMID:15637075]
EXISTENCE:mutant phenotype:GO:0015847 putrescine transport [PMID:9920864]
EXISTENCE:mutant phenotype:GO:0015848 spermidine transport [PMID:11171066|PMID:15637075|PMID:9920864]
EXISTENCE:mutant phenotype:GO:1902047 polyamine transmembrane transport [PMID:24136413]
Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane
Fe-binding Fe/S cluster assembly protein ISA1
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:21987576]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10805735]
EXISTENCE:genetic interaction:GO:0009102 biotin biosynthetic process [PMID:17259550]
EXISTENCE:genetic interaction:GO:0016226 iron-sulfur cluster assembly [PMID:10805735]
EXISTENCE:mutant phenotype:GO:0009102 biotin biosynthetic process [PMID:17259550]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10748136|PMID:10805735]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:12065597]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:21987576]
Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins; human ISCA1 can complement isa1 null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10467108]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10467108|PMID:22842922]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:16135516]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:direct assay:GO:0035617 stress granule disassembly [PMID:24291094]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16135516]
EXISTENCE:direct assay:GO:0051085 chaperone cofactor-dependent protein refolding [PMID:9674429]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:9674429]
EXISTENCE:direct assay:GO:0072380 TRC complex [PMID:20850366]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:11867765]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:16135516|PMID:9674429]
EXISTENCE:mutant phenotype:GO:0034975 protein folding in endoplasmic reticulum [PMID:10931304]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:7984243]
EXISTENCE:mutant phenotype:GO:0043531 ADP binding [PMID:11867765]
EXISTENCE:mutant phenotype:GO:0070370 cellular heat acclimation [PMID:2188365]
EXISTENCE:mutant phenotype:GO:0070414 trehalose metabolism in response to heat stress [PMID:9797333]
Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responds to stresses including heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation
ARS1206; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole; YLL025W is not an essential gene
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:25853343]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10745074]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:10893257]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8755907]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16806052]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16806052]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:16413483]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:7867784]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:8755907]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:27178214]
EXISTENCE:genetic interaction:GO:0051082 unfolded protein binding [PMID:9789005]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9448096]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:8754838]
EXISTENCE:mutant phenotype:GO:0035719 tRNA import into nucleus [PMID:25853343|PMID:33074312]
HSP70 family ATP-binding protein; involved in protein folding, vacuolar import of proteins; required for ubiquitin-dependent degradation of short-lived proteins; associated with chaperonin-containing T-complex; 98% identical to paralog Ssa1p with distinct functional specificity in propagation of yeast [URE3] prions and vacuolar-mediated degradation of gluconeogenesis enzymes; binds tRNA, has role in tRNA nuclear import during starvation; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:20498018]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:20498018]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20498018|PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:20498018]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:20498018]
Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000123 histone acetyltransferase complex [PMID:14761951|PMID:15099519]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14761951|PMID:14562095]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:15099519]
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:14761951]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:15099519]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:14761951|PMID:15099519]
EXISTENCE:mutant phenotype:GO:0000123 histone acetyltransferase complex [PMID:14761951]
EXISTENCE:physical interaction:GO:0000123 histone acetyltransferase complex [PMID:14761951]
EXISTENCE:physical interaction:GO:0016573 histone acetylation [PMID:14761951]
Non-essential component of the HAT-B histone acetyltransferase complex; localized to the nucleus; has a role in telomeric silencing; other members are Hat1p and Hat2p
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9214378]
EXISTENCE:direct assay:GO:0000133 polarisome [PMID:9632790]
EXISTENCE:direct assay:GO:0005078 MAP-kinase scaffold activity [PMID:12361575]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12361575|PMID:2647769]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9214378]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:23673619]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9214378|PMID:2647769]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:genetic interaction:GO:0031382 mating projection formation [PMID:9443897]
EXISTENCE:genetic interaction:GO:0036267 invasive filamentous growth [PMID:9443897]
EXISTENCE:genetic interaction:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:12857882]
EXISTENCE:mutant phenotype:GO:0005078 MAP-kinase scaffold activity [PMID:12361575]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:8909546]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077|PMID:9443897]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8013906|PMID:8909546]
EXISTENCE:mutant phenotype:GO:0031384 regulation of initiation of mating projection growth [PMID:14734532]
EXISTENCE:mutant phenotype:GO:0031385 regulation of termination of mating projection growth [PMID:14734532]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:10085294|PMID:11740491|PMID:12857882|PMID:8909546|PM ID:9571251|PMID:9632790|PMID:16166638]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:12857882|PMID:19633059]
Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:25631054|PMID:8910305]
EXISTENCE:direct assay:GO:0004713 protein tyrosine kinase activity [PMID:8910305]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22364741]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22364741]
EXISTENCE:direct assay:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:22364741]
EXISTENCE:genetic interaction:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:22364741]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:8910305]
EXISTENCE:mutant phenotype:GO:0004713 protein tyrosine kinase activity [PMID:8910305]
EXISTENCE:mutant phenotype:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:22364741]
Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:17237235]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:12171940]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12171940]
EXISTENCE:direct assay:GO:0030001 metal ion transport [PMID:12171940]
EXISTENCE:direct assay:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:12171940]
Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:12888345]
EXISTENCE:direct assay:GO:0004815 aspartate-tRNA ligase activity [PMID:8263943]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16113655]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16113655]
EXISTENCE:direct assay:GO:0006422 aspartyl-tRNA aminoacylation [PMID:8263943]
Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation; shares five highly conserved amino acids with human that when mutated cause leukoencephalopathy characterized by hypomyelination with brain stem and spinal cord involvement and leg spasticity (HBSL)
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:2188363]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:1986220]
Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10747953]
Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
ATP-binding cassette bilirubin transporter BPT1
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12455987|PMID:23658021]
EXISTENCE:direct assay:GO:0015086 cadmium ion transmembrane transporter activity [PMID:12455987]
EXISTENCE:direct assay:GO:0015691 cadmium ion transport [PMID:12455987]
EXISTENCE:genetic interaction:GO:0015127 bilirubin transmembrane transporter activity [PMID:10790694]
EXISTENCE:genetic interaction:GO:0015723 bilirubin transport [PMID:10790694]
EXISTENCE:mutant phenotype:GO:0015127 bilirubin transmembrane transporter activity [PMID:10790694]
EXISTENCE:mutant phenotype:GO:0015723 bilirubin transport [PMID:10790694]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:23658021]
ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
EXISTENCE:direct assay:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:16317069]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11101532|PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12730603]
EXISTENCE:direct assay:GO:0032473 cytoplasmic side of mitochondrial outer membrane [PMID:17210948]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11101532]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:17210948]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:18523582]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:17210948]
EXISTENCE:mutant phenotype:GO:0010795 regulation of ubiquinone biosynthetic process [PMID:29248374]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:26051939]
EXISTENCE:mutant phenotype:GO:0051646 mitochondrion localization [PMID:17210948]
Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochondria during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins; translationally represses oxidative stress-linked genes; regulates CoQ biosynthesis
EXISTENCE:direct assay:GO:0004771 sterol esterase activity [PMID:15713625]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:15713625|PMID:14562095]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15713625]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:15713625]
Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15367670|PMID:8508778]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:8508778]
Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:10777497]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10777497]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12090248]
EXISTENCE:genetic interaction:GO:0071472 cellular response to salt stress [PMID:10777497]
EXISTENCE:genetic interaction:GO:1904262 negative regulation of TORC1 signaling [PMID:30142151]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:30142151]
Plasma membrane-associated protein phosphatase; involved in the general stress response and the inactivating dephosphorylation of Mep2p; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr2p; member of the HAD family of protein phosphatases
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:9407107]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9407107]
EXISTENCE:direct assay:GO:0015680 protein maturation by copper ion transfer [PMID:15199057]
EXISTENCE:direct assay:GO:0016531 copper chaperone activity [PMID:15199057]
EXISTENCE:direct assay:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:15199057]
EXISTENCE:mutant phenotype:GO:0016531 copper chaperone activity [PMID:9585572]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:8662933]
Copper metallochaperone that transfers copper to Sco1p and Cox11p; eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs; interacts with the MICOS complex, and interaction is promoted by copper ions; human homolog COX17 partially complements yeast null mutant
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:1454790]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:1454790]
Nucleolar DEAD-box protein required for ribosome assembly and function; including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25598154]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25598154]
EXISTENCE:genetic interaction:GO:0035556 intracellular signal transduction [PMID:25598154]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:25598154]
EXISTENCE:mutant phenotype:GO:0035556 intracellular signal transduction [PMID:25598154]
Homolog of mammalian ELMO (Engulfment and celL MOtility); upstream component for regulation through the small GTPase Rho5p; may form a complex with Dck1p that acts as a GEF for Rho5p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:11266455|PMID:16407407]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:19556461]
EXISTENCE:direct assay:GO:0032865 ERMES complex [PMID:19556461|PMID:17410204]
EXISTENCE:direct assay:GO:0120013 lipid transfer activity [PMID:29279306]
EXISTENCE:genetic interaction:GO:0015914 phospholipid transport [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:11266455]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:8089172]
EXISTENCE:mutant phenotype:GO:0015914 phospholipid transport [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0015917 aminophospholipid transport [PMID:27469264]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:17410204]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:17410204]
EXISTENCE:mutant phenotype:GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering [PMID:19556461]
EXISTENCE:physical interaction:GO:0032865 ERMES complex [PMID:13679517]
ER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase; localizes to the peroxisome in glucose
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
Meiosis-specific hypothetical protein; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
ARS1207; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10783164|PMID:9554851]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16716188]
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
EXISTENCE:direct assay:GO:0005825 half bridge of spindle pole body [PMID:14504268]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:10455233]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:14504268]
EXISTENCE:mutant phenotype:GO:0051225 spindle assembly [PMID:17392514]
Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802|PMID:14562095]
EXISTENCE:direct assay:GO:0010484 histone H3 acetyltransferase activity [PMID:17272722|PMID:17272723]
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:17272723]
EXISTENCE:genetic interaction:GO:0010484 histone H3 acetyltransferase activity [PMID:18458063]
EXISTENCE:genetic interaction:GO:0016573 histone acetylation [PMID:17272722|PMID:18458063]
EXISTENCE:genetic interaction:GO:0043007 maintenance of rDNA [PMID:23593017]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19620280]
EXISTENCE:mutant phenotype:GO:0010468 regulation of gene expression [PMID:26941319]
EXISTENCE:mutant phenotype:GO:0010484 histone H3 acetyltransferase activity [PMID:17046836|PMID:17272722|PMID:17272723|PMID:18458063]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:17046836|PMID:17272723|PMID:18458063|PMID:17272722]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:23357952]
EXISTENCE:mutant phenotype:GO:2001032 regulation of double-strand break repair via nonhomologous end joining [PMID:18036332|PMID:27222517]
Histone acetyltransferase; critical for cell survival in presence of DNA damage during S phase, required for recovery after DSB repair; acetylates H3K56, H3K9; H3K56 acetylation activity required for expression homeostasis, buffering of mRNA synthesis rate against changes in gene dosage during S phase; involved in non-homologous end joining and regulation of Ty1 transposition; prevents hyper-amplification of rDNA; interacts physically with Vps75p
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16186251]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:26928762]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:11038182]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16968746]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16272155]
EXISTENCE:direct assay:GO:0042802 identical protein binding [PMID:16186251]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:17336903]
EXISTENCE:genetic interaction:GO:0000266 mitochondrial fission [PMID:10559943]
EXISTENCE:genetic interaction:GO:0007031 peroxisome organization [PMID:16968746]
EXISTENCE:genetic interaction:GO:0016559 peroxisome fission [PMID:18445678]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:23341591]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:10559943]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:16272155]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:9786946]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:16968746]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:32265272]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and inheritance; self assembles on the cytoplasmic face of mitochondrial tubules at sites where division will occur; participates in endocytosis and regulates peroxisome fission along with Vps1p; mutants in the human ortholog DNM1L, which mediates mitochondrial fission, peroxisomal division, autophagy, and mitophagy, are associated with slowly progressive infantile encephalopathy
ARS1208; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12110182]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12110182]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:11371346]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:22421151]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11371346]
EXISTENCE:direct assay:GO:0006270 DNA replication initiation [PMID:12110182]
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:12110182]
EXISTENCE:direct assay:GO:0030691 Noc2p-Noc3p complex [PMID:11371346]
EXISTENCE:mutant phenotype:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:22421151]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11371346]
Subunit of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
ARS1209; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16850348]
Putative plasma membrane permease; proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
EXISTENCE:direct assay:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:8855246]
EXISTENCE:direct assay:GO:0000439 transcription factor TFIIH core complex [PMID:19818408|PMID:14500720]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408|PMID:27381459]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19818408]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:15837426]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:7891722]
Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p
mitogen-activated protein kinase kinase kinase SSK1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14665464]
EXISTENCE:direct assay:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:8808622]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:9482735]
EXISTENCE:direct assay:GO:0031954 positive regulation of protein autophosphorylation [PMID:9482735]
EXISTENCE:direct assay:GO:0071474 cellular hyperosmotic response [PMID:9482735]
EXISTENCE:direct assay:GO:0140531 regulation of osmosensory signaling MAPK cascade [PMID:9482735]
EXISTENCE:genetic interaction:GO:0140531 regulation of osmosensory signaling MAPK cascade [PMID:9482735]
EXISTENCE:mutant phenotype:GO:0000156 phosphorelay response regulator activity [PMID:8183345]
EXISTENCE:mutant phenotype:GO:0030295 protein kinase activator activity [PMID:9482735]
EXISTENCE:mutant phenotype:GO:0071474 cellular hyperosmotic response [PMID:18573873]
EXISTENCE:mutant phenotype:GO:0140531 regulation of osmosensory signaling MAPK cascade [PMID:18573873]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:19633059]
EXISTENCE:physical interaction:GO:0031435 mitogen-activated protein kinase kinase kinase binding [PMID:9482735]
Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11927594]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:11927594]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391]
EXISTENCE:direct assay:GO:0030915 Smc5-Smc6 complex [PMID:15738391]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:17923688]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391]
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:14638855]
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:16027163]
EXISTENCE:direct assay:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:14517234]
EXISTENCE:mutant phenotype:GO:0001671 ATPase activator activity [PMID:14605210|PMID:14638855]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:14517234|PMID:14638855|PMID:14605210]
Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
ATPase-activating ribosome biosynthesis protein
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:23185031]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:20670889]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17293872]
EXISTENCE:direct assay:GO:0061770 translation elongation factor binding [PMID:31006804]
EXISTENCE:genetic interaction:GO:0007004 telomere maintenance via telomerase [PMID:11230140]
EXISTENCE:genetic interaction:GO:0016233 telomere capping [PMID:11230140]
EXISTENCE:mutant phenotype:GO:0003711 transcription elongation factor activity [PMID:31006804]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:31006804]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:11230140]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:11230140]
EXISTENCE:mutant phenotype:GO:0032205 negative regulation of telomere maintenance [PMID:19532124]
EXISTENCE:physical interaction:GO:1990879 CST complex [PMID:11230140]
Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p; similar to human Ten1 which is critical for the telomeric function of the CST (Cdc13p-Stn1p-Ten1p) complex
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0003955 NAD(P)H dehydrogenase (quinone) activity [PMID:17298444]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17298444|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17298444]
EXISTENCE:direct assay:GO:0042994 cytoplasmic sequestering of transcription factor [PMID:19029946]
EXISTENCE:mutant phenotype:GO:0003955 NAD(P)H dehydrogenase (quinone) activity [PMID:17298444]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:19709309]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17298444]
FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
hypothetical protein; YLR012C is not an essential gene
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT4, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9858611]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9858611]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:7843415]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:7958913]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:2204810]
EXISTENCE:mutant phenotype:GO:1900399 positive regulation of pyrimidine nucleotide biosynthetic process [PMID:24189721]
Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:direct assay:GO:0051568 histone H3-K4 methylation [PMID:11742990|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:11742990]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11687631|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0051568 histone H3-K4 methylation [PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0080182 histone H3-K4 trimethylation [PMID:16921172|PMID:16980427]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:15565172]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0070274 RES complex [PMID:15565172]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:15565172]
EXISTENCE:mutant phenotype:GO:0051237 maintenance of RNA location [PMID:16162818]
Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8807288]
EXISTENCE:direct assay:GO:0017061 S-methyl-5-thioadenosine phosphorylase activity [PMID:14506228]
EXISTENCE:direct assay:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:14506228]
EXISTENCE:mutant phenotype:GO:0006537 glutamate biosynthetic process [PMID:8807288]
EXISTENCE:mutant phenotype:GO:0017061 S-methyl-5-thioadenosine phosphorylase activity [PMID:14506228]
EXISTENCE:mutant phenotype:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:14506228|PMID:18625006]
Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
EXISTENCE:curator inference:GO:0017056 structural constituent of nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0005640 nuclear outer membrane [PMID:16361228]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:direct assay:GO:0070762 nuclear pore transmembrane ring [PMID:10684247|PMID:19414609]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:16361228]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:19414609]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:19571182|PMID:20713690]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:16361228]
EXISTENCE:physical interaction:GO:0070762 nuclear pore transmembrane ring [PMID:19414609]
Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
Putative plasma membrane phosphatase; involved in the general stress response and inactivating dephosphorylation of Mep2p; required along with Psr1p and binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr1p; member of the HAD family of protein phosphatases
EXISTENCE:direct assay:GO:0004771 sterol esterase activity [PMID:15632184|PMID:15713625]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15632184]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:14587103]
EXISTENCE:mutant phenotype:GO:0004771 sterol esterase activity [PMID:15632184]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:15632184|PMID:15713625]
Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17707236]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17707236]
EXISTENCE:genetic interaction:GO:0051131 chaperone-mediated protein complex assembly [PMID:17707236]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:18278055|PMID:18260085]
EXISTENCE:mutant phenotype:GO:0051131 chaperone-mediated protein complex assembly [PMID:17707236]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:17660569]
EXISTENCE:physical interaction:GO:0051131 chaperone-mediated protein complex assembly [PMID:18278055]
Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:23831625]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:19350533]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:19350533]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:19350533]
EXISTENCE:direct assay:GO:1990932 5.8S rRNA binding [PMID:19350533]
EXISTENCE:mutant phenotype:GO:0042256 cytosolic ribosome assembly [PMID:17353896]
Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:15060275]
Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:15504724]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:15504724]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15504724]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15504724]
EXISTENCE:genetic interaction:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:20462952]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:15504724]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:15504724]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15504724|PMID:18070918]
EXISTENCE:physical interaction:GO:1990304 MUB1-RAD6-UBR2 ubiquitin ligase complex [PMID:18070918]
Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex
EXISTENCE:curator inference:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15086794]
EXISTENCE:direct assay:GO:0000815 ESCRT III complex [PMID:12194857]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9606181]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27803246]
EXISTENCE:direct assay:GO:0061709 reticulophagy [PMID:31802527]
EXISTENCE:direct assay:GO:1904902 ESCRT III complex assembly [PMID:31273031]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:9606181]
EXISTENCE:mutant phenotype:GO:0070676 intralumenal vesicle formation [PMID:19234443]
EXISTENCE:mutant phenotype:GO:0071454 cellular response to anoxia [PMID:20402793]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15086794|PMID:27074665]
One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11001058|PMID:11959998]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:9725919]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1400588]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:11001058|PMID:11959998]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9545229]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:7596416]
EXISTENCE:mutant phenotype:GO:0048280 vesicle fusion with Golgi apparatus [PMID:9545229]
cis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins; plays a role in autophagy by regulating formation of autophagic vesicles in Golgi
EXISTENCE:direct assay:GO:0004069 L-aspartate:2-oxoglutarate aminotransferase activity [PMID:1859361]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:9288922]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9288922]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:35621265]
EXISTENCE:mutant phenotype:GO:0004069 L-aspartate:2-oxoglutarate aminotransferase activity [PMID:9288922]
EXISTENCE:mutant phenotype:GO:0005777 peroxisome [PMID:9288922]
EXISTENCE:mutant phenotype:GO:0006532 aspartate biosynthetic process [PMID:9288922|PMID:15780654]
EXISTENCE:mutant phenotype:GO:0032938 negative regulation of translation in response to oxidative stress [PMID:35621265]
Cytosolic aspartate aminotransferase involved in the metabolism of nitrogen and amino acids such as aspartate; localizes to and functions in peroxisomes in oleate-grown cells; moonlighting role as a ribosome-associated protein that modulates translation in response to stress
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:8455623]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:8455623]
EXISTENCE:direct assay:GO:0051099 positive regulation of binding [PMID:24130456]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:14749391|PMID:8455623]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:2898766|PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA); essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA
bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE16
EXISTENCE:direct assay:GO:0003937 IMP cyclohydrolase activity [PMID:10877846]
EXISTENCE:direct assay:GO:0004643 phosphoribosylaminoimidazolecarboxamide formyltransferase activity [PMID:10877846]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10877846]
EXISTENCE:direct assay:GO:0006164 purine nucleotide biosynthetic process [PMID:10877846]
EXISTENCE:direct assay:GO:0006189 'de novo' IMP biosynthetic process [PMID:10877846]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:10877846]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:10877846]
Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:6814480]
Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
hypothetical protein; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W
hypothetical protein; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W; YLR031W has a paralog, YMR124W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:19706603]
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:17936713]
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:17936713]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:24393774]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10880451]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0006301 postreplication repair [PMID:17936713]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:17936713]
EXISTENCE:direct assay:GO:0009378 four-way junction helicase activity [PMID:17936713]
EXISTENCE:direct assay:GO:0010994 free ubiquitin chain polymerization [PMID:19706603]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:18757916]
EXISTENCE:direct assay:GO:0070987 error-free translesion synthesis [PMID:18757916]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:16224103]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16224103]
EXISTENCE:mutant phenotype:GO:0042275 error-free postreplication DNA repair [PMID:19079240]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:23142547|PMID:18757916]
EXISTENCE:mutant phenotype:GO:0070987 error-free translesion synthesis [PMID:18757916]
DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, and error-prone translesion synthesis; required for UV and MMS-associated unequal sister chromatid exchange (SCE) but not for double-strand break associated unequal SCE; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; human homolog HLTF can complement yeast null mutant
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12052880]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:12052880]
Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
putative divalent metal ion transporter SMF3
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11027260|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006826 iron ion transport [PMID:17681937]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:11027260]
Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of Nramp family of metal transport proteins; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:28051769]
EXISTENCE:direct assay:GO:0000405 bubble DNA binding [PMID:28051769]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:10679328]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15467363]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:10570173]
EXISTENCE:physical interaction:GO:0032390 MutLbeta complex [PMID:10570173]
Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p, plays accessory role in stimulating activity of MLH1/PMS1 complex
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); YLR035C-A is part of a mutant retrotransposon
Putative protein predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein
ARS1210; Autonomously replicating sequence
Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:1331058]
Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly and activity of cytochrome c oxidase; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10990452]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10990452]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:24924636]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:10990452]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10990452]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:10990452]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:11160819]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:10990452]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10990452]
EXISTENCE:physical interaction:GO:0034066 Ric1-Rgp1 guanyl-nucleotide exchange factor complex [PMID:10990452]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:10990452]
Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:24121774]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:24121774]
MATalpha-specific a-factor blocker; contributes to mating efficiency under certain conditions; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W
EXISTENCE:mutant phenotype:GO:0060258 negative regulation of filamentous growth [PMID:32665277]
Negative regulator of the filamentous growth pathway; inhibits invasive growth by dampening the activity of the filamentous MAPK pathway; localizes to the cell wall and to the vacuole and cell periphery in HTP studies; deletion improves xylose fermentation in industrially engineered strains; nonessential gene
ARS1211; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8603912|PMID:26928762]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9813082]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9813082]
EXISTENCE:direct assay:GO:0015036 disulfide oxidoreductase activity [PMID:19362171]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:9657146]
EXISTENCE:direct assay:GO:0080058 protein deglutathionylation [PMID:20074363]
EXISTENCE:genetic interaction:GO:0045454 cell redox homeostasis [PMID:22561702]
EXISTENCE:genetic interaction:GO:0080058 protein deglutathionylation [PMID:20074363]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:8603912|PMID:9015301]
EXISTENCE:physical interaction:GO:0005829 cytosol [PMID:9159115]
Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system that protects cells from oxidative and reductive stress; subunit of the LMA1 complex, with Pbi2p, that promotes vacuole inheritance; cofactor for Tsa1p; required for ER-Golgi transport; facilitates mitochondrial import of small Tims (Tim9p, Tim10p, Tim13p) by maintaining them in reduced form along with Trx2p; component of a signal transduction redox relay in a light sensing pathway; abundance increases under DNA replication stress
EXISTENCE:direct assay:GO:0004737 pyruvate decarboxylase activity [PMID:23423327|PMID:2404950]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16850348|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:2665820]
EXISTENCE:direct assay:GO:0006090 pyruvate metabolic process [PMID:2404950]
EXISTENCE:direct assay:GO:0019655 glycolytic fermentation to ethanol [PMID:2404950]
EXISTENCE:direct assay:GO:0046809 replication compartment [PMID:31648290]
EXISTENCE:genetic interaction:GO:0000949 aromatic amino acid family catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006559 L-phenylalanine catabolic process [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006569 tryptophan catabolic process [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0045069 regulation of viral genome replication [PMID:31648290]
EXISTENCE:mutant phenotype:GO:0047433 branched-chain-2-oxoacid decarboxylase activity [PMID:9546164]
Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo; protein tyrosine nitration on Tyr157 or Tyr344 inhibits activity and impairs fermentation
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11511347|PMID:11553724]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:9606209]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11511347]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11511347]
EXISTENCE:direct assay:GO:0046785 microtubule polymerization [PMID:25172511]
EXISTENCE:direct assay:GO:0061863 microtubule plus end polymerase [PMID:25172511]
EXISTENCE:genetic interaction:GO:0007052 mitotic spindle organization [PMID:11756468]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:33752231]
EXISTENCE:mutant phenotype:GO:0008017 microtubule binding [PMID:11553724]
Microtubule polymerase of the XMAP215/Dis1 family; required for elongation of mitotic spindle during anaphase, as well as for spindle orientation and metaphase chromosome alignment; stabilizes or destabilizes kinetochore-microtubule attachment depending on kinetochore tension; interacts with spindle pole body component Spc72p; metazoan ch-TOG/XMAP215 family member
Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YLR046C has a paralog, RTA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
EXISTENCE:mutant phenotype:GO:0006875 cellular metal ion homeostasis [PMID:14534306]
EXISTENCE:mutant phenotype:GO:0016722 oxidoreductase activity, acting on metal ions [PMID:14534306]
Protein with sequence similarity to iron/copper reductases; involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:8626693]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:8626693]
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:8626693]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:8626693]
Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:30213914|PMID:33596095]
EXISTENCE:mutant phenotype:GO:0032594 protein transport within lipid bilayer [PMID:30213914]
Integral ER membrane protein involved in protein sorting; component of ER-SURF, a pathway involved in retrieving mitochondrial membrane protein precursors and rerouting them to the mitochondrial translocation system; promotes the proteolytic degradation of non-productive mitochondrial precursor proteins suggesting involvement in quality control during protein import; GFP-fusion protein localizes to the ER; member of a conserved family of ER proteins including human homolog TMEM97
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095|PMID:14690591]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320]
Nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon; essential gene
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:12887900]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:17562861]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:17562861]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Subunit of the INO80 chromatin remodeling complex
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:35239649]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:35239649]
EXISTENCE:direct assay:GO:0006995 cellular response to nitrogen starvation [PMID:35239649]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:35239649]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:35239649]
EXISTENCE:expression pattern:GO:0006995 cellular response to nitrogen starvation [PMID:35239649]
EXISTENCE:mutant phenotype:GO:0006995 cellular response to nitrogen starvation [PMID:35239649]
EXISTENCE:mutant phenotype:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:35239649]
EXISTENCE:physical interaction:GO:0044877 protein-containing complex binding [PMID:35239649]
Nitrogen-responsive activator of Start; nuclear microprotein with transcriptional activator activity; bypasses Start arrest in a cln3 bck2 double mutant when overexpressed; interacts with subunits of the SBF complex (Swi4p-Swi6p); induced by nitrogen limitation and rapamycin with peak abundance at Start; proposed to promote the G1/S transition by rewiring the G1/S transcriptional machinery under poor nitrogen conditions upstream of the Whi5-inhibited form of SBF and independent of Cln3 and Bck2
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:15590821]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15590821|PMID:24390141]
hypothetical protein reputedly involved in spore wall assembly
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10611242]
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:15132995]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10611242]
EXISTENCE:mutant phenotype:GO:0003712 transcription coregulator activity [PMID:9858534]
EXISTENCE:mutant phenotype:GO:0017025 TBP-class protein binding [PMID:9858534]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12370284]
Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters
EXISTENCE:direct assay:GO:0000248 C-5 sterol desaturase activity [PMID:34600]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:21737688]
EXISTENCE:mutant phenotype:GO:0000248 C-5 sterol desaturase activity [PMID:1864507]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:1864507]
C-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of HRD ubiquitin ligase; mutation is functionally complemented by human SC5D
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:21971548]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:21971548]
Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12700234]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:mutant phenotype:GO:0004372 glycine hydroxymethyltransferase activity [PMID:8132653|PMID:9398220]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:8132653]
EXISTENCE:mutant phenotype:GO:0006730 one-carbon metabolic process [PMID:8132653|PMID:9398220]
Cytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9933355|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:9933355]
EXISTENCE:genetic interaction:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10716935]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:10716935]
EXISTENCE:genetic interaction:GO:0034476 U5 snRNA 3'-end processing [PMID:10716935]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:9933355]
EXISTENCE:mutant phenotype:GO:0000175 3'-5'-exoribonuclease activity [PMID:10716935]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9933355]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10716935]
3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
EXISTENCE:direct assay:GO:0004826 phenylalanine-tRNA ligase activity [PMID:16162501|PMID:166841]
EXISTENCE:direct assay:GO:0006432 phenylalanyl-tRNA aminoacylation [PMID:16162501|PMID:166841]
EXISTENCE:direct assay:GO:0009328 phenylalanine-tRNA ligase complex [PMID:3049607]
Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:27478489]
Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; required for the oxidative stress response, pseudohyphal and invasive growth; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
25S rRNA (uracil2843-N3)-methyltransferase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24335083|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0070042 rRNA (uridine-N3-)-methyltransferase activity [PMID:24335083]
EXISTENCE:mutant phenotype:GO:0070475 rRNA base methylation [PMID:24335083]
Methyltransferase required for m3U2843 methylation of the 25S rRNA; S-adenosylmethionine-dependent; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:21912603]
Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Pho88p/Snd3p and Sec61p-translocon subunits; can compensate for loss of SRP; role in the late endosome-vacuole interface; putative role in secretory protein quality control
EXISTENCE:direct assay:GO:0005787 signal peptidase complex [PMID:1846444|PMID:8910564]
EXISTENCE:direct assay:GO:0006465 signal peptide processing [PMID:9148931]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:9148930|PMID:9148931]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:9148931]
Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:25181249]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9692914]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:7664742]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:7664742]
EXISTENCE:mutant phenotype:GO:0070134 positive regulation of mitochondrial translational initiation [PMID:18039654|PMID:7664742]
Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs)
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]
Nucleolar protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:1935960]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:1935960]
Mitochondrial elongation factor involved in translational elongation
EXISTENCE:direct assay:GO:0005999 xylulose biosynthetic process [PMID:10486580]
EXISTENCE:direct assay:GO:0046526 D-xylulose reductase activity [PMID:10486580]
Xylitol dehydrogenase; converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
EXISTENCE:direct assay:GO:0001093 TFIIB-class transcription factor binding [PMID:27688401]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11470794]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
EXISTENCE:direct assay:GO:0032366 intracellular sterol transport [PMID:25987606]
EXISTENCE:direct assay:GO:0044233 mitochondria-associated endoplasmic reticulum membrane [PMID:25987606|PMID:26001273|PMID:26119743]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:25987606|PMID:26001273|PMID:26119743]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:25987606]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:26119743]
Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:19410548]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:24962567|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0010992 ubiquitin recycling [PMID:19410548]
Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11452010|PMID:23212245]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23045392|PMID:23212245]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23045392|PMID:23212245]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:23045392]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:23045392|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:23045392|PMID:23212245]
C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:18824477|PMID:9580698]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:18824477]
Ribosomal 60S subunit protein L10; homologous to mammalian ribosomal protein L10 and bacterial L16; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; protein abundance increases under DNA replication stress; mutations in human homolog implicated in T-cell acute lymphoblastic leukemia and also autism spectrum disorders (ASD); human RPL10 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:21168530]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:21168530]
Protein required for assembly of respiratory complex III; mitochondrial inner membrane protein; required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11001058]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:1396561]
EXISTENCE:direct assay:GO:0012507 ER to Golgi transport vesicle membrane [PMID:8500167]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1396561]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:11001058]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8500167]
EXISTENCE:mutant phenotype:GO:0048280 vesicle fusion with Golgi apparatus [PMID:8500167]
v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi; required for efficient nuclear fusion during mating
cyclin-dependent protein serine/threonine kinase inhibiting protein SIC1
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7954792]
EXISTENCE:direct assay:GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity [PMID:7954792|PMID:8421781]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155|PMID:11792824|PMID:16294029]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11792824]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:7954792]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7954792]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:20417603]
Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12134087]
EXISTENCE:direct assay:GO:0030246 carbohydrate binding [PMID:16439369]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12134087]
EXISTENCE:physical interaction:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:12857885]
Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication
ARS1212; Late-firing chromosomal replication origin
EXISTENCE:direct assay:GO:0005355 glucose transmembrane transporter activity [PMID:12702270]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9045811]
EXISTENCE:mutant phenotype:GO:0005354 galactose transmembrane transporter activity [PMID:3082856]
EXISTENCE:mutant phenotype:GO:0006012 galactose metabolic process [PMID:3082856]
EXISTENCE:mutant phenotype:GO:0015757 galactose transmembrane transport [PMID:3082856]
Galactose permease; required for utilization of galactose; also able to transport glucose
EXISTENCE:mutant phenotype:GO:0006139 nucleobase-containing compound metabolic process [PMID:9744871]
hypothetical protein; overexpression suppresses the lethality caused by a rad53 null mutation
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20681974]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:8314797]
EXISTENCE:genetic interaction:GO:0006878 cellular copper ion homeostasis [PMID:20681974]
EXISTENCE:genetic interaction:GO:0007034 vacuolar transport [PMID:20526336]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:18178563]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:18178563]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:20681974]
EXISTENCE:mutant phenotype:GO:0016197 endosomal transport [PMID:20526336]
Protein with a role in cellular adhesion and filamentous growth; also endosome-to-vacuole sorting; similar to Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments; EMP70 has a paralog, TMN2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:15356260|PMID:11110666|PMID:25416945]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15356260|PMID:11110666|PMID:26928762]
EXISTENCE:genetic interaction:GO:0007121 bipolar cellular bud site selection [PMID:11110666]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:29074565]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:11110666]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:25416945]
Protein required for the maintenance of bud site selection; localizes; localization to the bud neck and previous sites of cell division (bud scars; cytokinesis remnants (CRMs)) is interdependent with Rax2p; required with Rax1p to anchor both Nba1p and Nis1p to CRMs where a Cdc42p inhibitory zone is established preventing repolarization of cells at previous division sites; RAX2 mRNA stability is regulated by Mpt5p; predicted type I membrane-spanning protein; N-glycosylated
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608|PMID:16299513]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11011149]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:14690608]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:16299513]
Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
EXISTENCE:curator inference:GO:0007130 synaptonemal complex assembly [PMID:10811823]
EXISTENCE:curator inference:GO:0010032 meiotic chromosome condensation [PMID:10811823]
EXISTENCE:curator inference:GO:0051307 meiotic chromosome separation [PMID:10811823]
EXISTENCE:direct assay:GO:0000796 condensin complex [PMID:10811823]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11864994]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12719426]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:10811823]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:10811823]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:18708579]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:10811823]
Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
EXISTENCE:mutant phenotype:GO:0006113 fermentation [PMID:10781556]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:19433630]
EXISTENCE:mutant phenotype:GO:0055091 phospholipid homeostasis [PMID:35188676]
Protein with structural similarity to lipid transport protein Vps13p; localizes to multiple membrane contact sites between organelles; required for fermentation at low temperature; plays a role in phosphatidylethanolamine metabolism and maturation of secretory proteins; non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0042765 GPI-anchor transamidase complex [PMID:11598210]
EXISTENCE:mutant phenotype:GO:0016255 attachment of GPI anchor to protein [PMID:11598210|PMID:7730400]
Subunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER; human homolog GPAA1 can complement growth defects of yeast thermosensitive mutant at restrictive temperature
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0030170 pyridoxal phosphate binding [PMID:29867852]
EXISTENCE:mutant phenotype:GO:0004021 L-alanine:2-oxoglutarate aminotransferase activity [PMID:19396236]
EXISTENCE:mutant phenotype:GO:0006523 alanine biosynthetic process [PMID:19396236]
EXISTENCE:mutant phenotype:GO:0006524 alanine catabolic process [PMID:19396236]
Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:23329686]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:23329686]
Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0099617 matrix side of mitochondrial inner membrane [PMID:30759361]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:19751518]
EXISTENCE:mutant phenotype:GO:0097745 mitochondrial tRNA 5'-end processing [PMID:30759361]
hypothetical protein; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine; localizes to the matrix side of the inner mitochondrial membrane
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:9055073]
EXISTENCE:mutant phenotype:GO:0008272 sulfate transport [PMID:9055073]
EXISTENCE:mutant phenotype:GO:0015116 sulfate transmembrane transporter activity [PMID:9055073]
High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16855025|PMID:26928762]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:19805279]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9425154]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:17400548|PMID:9685264]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:12730205]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:17400548]
v-SNARE component of vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in vacuolar membrane; targeted to vacuole via AP-3 pathway
EXISTENCE:mutant phenotype:GO:0035556 intracellular signal transduction [PMID:10628841]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12482963]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12482963]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:12482963]
EXISTENCE:direct assay:GO:0036437 Isw1b complex [PMID:12482963]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12482963]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:12482963]
Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8203145]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8020610]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:27096577]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:27096577]
EXISTENCE:genetic interaction:GO:0045921 positive regulation of exocytosis [PMID:15563607]
EXISTENCE:genetic interaction:GO:1903896 positive regulation of IRE1-mediated unfolded protein response [PMID:25348718]
EXISTENCE:physical interaction:GO:0005886 plasma membrane [PMID:15563607]
EXISTENCE:physical interaction:GO:0045921 positive regulation of exocytosis [PMID:15563607]
S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
SCF ubiquitin ligase complex subunit HRT3
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:10582239|PMID:14747994]
EXISTENCE:genetic interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:10582239]
EXISTENCE:mutant phenotype:GO:0071406 cellular response to methylmercury [PMID:17141224]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:17141224|PMID:14747994]
Putative SCF-ubiquitin ligase F-box protein; based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:30520011]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:8889513]
EXISTENCE:mutant phenotype:GO:0009063 amino acid catabolic process [PMID:8889513]
EXISTENCE:mutant phenotype:GO:0045893 positive regulation of DNA-templated transcription [PMID:8889513]
DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
lysophosphatidic acid acyltransferase ICT1
EXISTENCE:direct assay:GO:0042171 lysophosphatidic acid acyltransferase activity [PMID:18252723]
EXISTENCE:mutant phenotype:GO:0006654 phosphatidic acid biosynthetic process [PMID:18252723]
Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication; human ABHD5 can complement ict1 null mutant
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:22467864]
EXISTENCE:genetic interaction:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:22467864]
EXISTENCE:genetic interaction:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:22467864]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:22467864]
EXISTENCE:physical interaction:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:22467864]
EXISTENCE:physical interaction:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:22467864]
Mitochondrial protein required for outer membrane protein import; involved in import of the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and Fzo1p; component of a large protein complex in the outer membrane that includes Mim1p and functions as cation-selective channel for translocation of positively charged precursor segments; not essential in W303 strain background
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11279045|PMID:26928762|PMID:12119386]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12119386]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
EXISTENCE:mutant phenotype:GO:0000253 3-keto sterol reductase activity [PMID:10535978]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:10535978]
3-keto sterol reductase; catalyzes last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; Erg27 protein is mainly ER-localized in strains devoid of mtDNA (rho cells); mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:12574115]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:12399376]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:12574115]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
ARS1212.5; Strong autonomously replicating sequence
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:15494305|PMID:9554851]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:12019222]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:23382391]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8901577]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9335335|PMID:9554851]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:2044962]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:23382391]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:2044962]
DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:19325107]
hypothetical protein; mutant is deficient in cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD); SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
EXISTENCE:direct assay:GO:0000213 tRNA-intron endonuclease activity [PMID:9200603]
EXISTENCE:direct assay:GO:0000214 tRNA-intron endonuclease complex [PMID:9200603]
EXISTENCE:direct assay:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:9200603]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407|PMID:12925762]
EXISTENCE:mutant phenotype:GO:0000213 tRNA-intron endonuclease activity [PMID:2182322]
EXISTENCE:mutant phenotype:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:2182322]
EXISTENCE:mutant phenotype:GO:0005741 mitochondrial outer membrane [PMID:12925762]
EXISTENCE:mutant phenotype:GO:0098787 mRNA cleavage involved in mRNA processing [PMID:25971974]
Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease; localizes to the peroxisome in a Pex5p-dependent manner in oleate
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position C1007
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12102729]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15528184]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:30460895]
EXISTENCE:direct assay:GO:0110136 protein-RNA complex remodeling [PMID:20542003]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:15528184]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:30460895]
EXISTENCE:mutant phenotype:GO:0110136 protein-RNA complex remodeling [PMID:20542003|PMID:24240281]
EXISTENCE:mutant phenotype:GO:2000200 regulation of ribosomal subunit export from nucleus [PMID:24240281]
Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain
EXISTENCE:mutant phenotype:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10716935]
RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0010501 RNA secondary structure unwinding [PMID:27484481]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:22408182]
EXISTENCE:mutant phenotype:GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) [PMID:17925343]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:24196718]
EXISTENCE:physical interaction:GO:0017069 snRNA binding [PMID:2440583]
U6 spliceosomal RNA (U6 snRNA); mediates catalysis of pre-mRNA splicing by the spliceosome; interacts with U4; homology to U6 snRNA, interacts with snr14
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10681558]
EXISTENCE:direct assay:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:10681558]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:15051715]
EXISTENCE:mutant phenotype:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:mutant phenotype:GO:0010038 response to metal ion [PMID:12270680]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:10681558]
Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:21320323]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:21320323]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:10322008|PMID:21320323]
EXISTENCE:mutant phenotype:GO:0000752 agglutination involved in conjugation with cellular fusion [PMID:10322008]
EXISTENCE:mutant phenotype:GO:0005199 structural constituent of cell wall [PMID:17315267|PMID:21320323]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10322008|PMID:15972461|PMID:21320323]
Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:direct assay:GO:0001100 negative regulation of exit from mitosis [PMID:32265285]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:24508389]
EXISTENCE:direct assay:GO:0004707 MAP kinase activity [PMID:10805732|PMID:32265285]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10817757]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10817757|PMID:11914276]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:23178807]
EXISTENCE:direct assay:GO:0033262 regulation of nuclear cell cycle DNA replication [PMID:23178807]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12743037]
EXISTENCE:mutant phenotype:GO:0006972 hyperosmotic response [PMID:7681220]
EXISTENCE:mutant phenotype:GO:0007231 osmosensory signaling pathway [PMID:7681220]
EXISTENCE:mutant phenotype:GO:0016241 regulation of macroautophagy [PMID:16874103]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:23435728|PMID:24508389]
EXISTENCE:physical interaction:GO:0005516 calmodulin binding [PMID:27421986]
Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
ARS1213; Autonomously Replicating Sequence
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006892 post-Golgi vesicle-mediated transport [PMID:17229886]
Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
cleavage polyadenylation factor subunit CFT2
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9099738]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:10523662|PMID:12819204]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0006379 mRNA cleavage [PMID:9099738]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
EXISTENCE:direct assay:GO:0000243 commitment complex [PMID:10376880|PMID:9150140]
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:9182766]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0045131 pre-mRNA branch point binding [PMID:9182766]
Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:11105756|PMID:11842115]
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:11105756]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11973290]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:11842115]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11973290]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11973290]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:11105756|PMID:11842115]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:11105756]
EXISTENCE:mutant phenotype:GO:0000354 cis assembly of pre-catalytic spliceosome [PMID:10445879|PMID:12509417]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:11973290]
EXISTENCE:physical interaction:GO:0000354 cis assembly of pre-catalytic spliceosome [PMID:10445879]
EXISTENCE:physical interaction:GO:0006270 DNA replication initiation [PMID:11973290]
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
ARS1214; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0008474 palmitoyl-(protein) hydrolase activity [PMID:12080046]
EXISTENCE:direct assay:GO:0035601 protein deacylation [PMID:12080046]
EXISTENCE:mutant phenotype:GO:0008474 palmitoyl-(protein) hydrolase activity [PMID:12080046]
EXISTENCE:mutant phenotype:GO:0035601 protein deacylation [PMID:12080046]
Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0000813 ESCRT I complex [PMID:15044434]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:11511343]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:11511343]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:11511343]
EXISTENCE:physical interaction:GO:0000813 ESCRT I complex [PMID:16615894|PMID:11511343]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:11511343|PMID:15086794]
Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
EXISTENCE:direct assay:GO:0004190 aspartic-type endopeptidase activity [PMID:8389368|PMID:8626758]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7657670]
EXISTENCE:genetic interaction:GO:0016485 protein processing [PMID:18591427]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:16087741]
EXISTENCE:genetic interaction:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:2183521]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:18591427]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:18591427]
Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor
EXISTENCE:direct assay:GO:0004190 aspartic-type endopeptidase activity [PMID:10191273]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11016834]
Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
hypothetical protein; conserved among S. cerevisiae strains; partially overlaps dubious ORF YLR123C
hypothetical protein; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
EXISTENCE:genetic interaction:GO:0007091 metaphase/anaphase transition of mitotic cell cycle [PMID:9430641]
EXISTENCE:genetic interaction:GO:0010458 exit from mitosis [PMID:9430641]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473|PMID:9430641]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0120151 positive regulation of mitotic actomyosin contractile ring disassembly [PMID:19109423]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
EXISTENCE:direct assay:GO:0031624 ubiquitin conjugating enzyme binding [PMID:18206966]
EXISTENCE:direct assay:GO:0032182 ubiquitin-like protein binding [PMID:15988528]
EXISTENCE:direct assay:GO:0045116 protein neddylation [PMID:15988528|PMID:18206966]
EXISTENCE:genetic interaction:GO:0045116 protein neddylation [PMID:15988528]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:18206966]
EXISTENCE:mutant phenotype:GO:0045116 protein neddylation [PMID:15988528|PMID:18206966]
EXISTENCE:mutant phenotype:GO:0051443 positive regulation of ubiquitin-protein transferase activity [PMID:15988528]
EXISTENCE:mutant phenotype:GO:0097602 cullin family protein binding [PMID:18206966]
Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
snoRNA-binding rRNA-processing protein DIP2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0034388 Pwp2p-containing subcomplex of 90S preribosome [PMID:15231838|PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
Nucleolar protein; specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
EXISTENCE:mutant phenotype:GO:0000007 low-affinity zinc ion transmembrane transporter activity [PMID:8798516]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:8798516]
EXISTENCE:mutant phenotype:GO:0071578 zinc ion import across plasma membrane [PMID:8798516]
Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:8657150]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15200949|PMID:1730413|PMID:22842922]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15200949|PMID:1730413]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10409653|PMID:1730413]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:8657150]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:27190003]
EXISTENCE:mutant phenotype:GO:0060196 positive regulation of antisense RNA transcription [PMID:27190003]
EXISTENCE:mutant phenotype:GO:2001043 positive regulation of septum digestion after cytokinesis [PMID:11259593]
Transcription factor required for septum destruction after cytokinesis; part of the RAM network that regulates polarity and morphogenesis; NES phosphorylation by RAM network kinase Cbk1p blocks nuclear exit in mother cells during the M/G1 transition, causing asymmetric localization to daughter cell nuclei, and increased Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; Spt16p is required for nuclear exclusion during G1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22027258|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22027258|PMID:14562095|PMID:14690591]
EXISTENCE:genetic interaction:GO:0034477 U6 snRNA 3'-end processing [PMID:22899009]
EXISTENCE:mutant phenotype:GO:0034477 U6 snRNA 3'-end processing [PMID:22899009]
Putative poly(U)-specific 3'-to-5' RNA exonuclease; involved in 3'-end processing of U6 snRNA removing uridines and generating a terminal 2',3' cyclic phosphate; essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants; homolog of S.pombe gene, mpn1 and human gene, hUSB1; mutations in hUSB1 are associated with a rare genodermatosis, poikiloderma with neutropenia (OMIM 604173)
bifunctional choline kinase/ethanolamine kinase CKI1
EXISTENCE:direct assay:GO:0004103 choline kinase activity [PMID:2536698]
EXISTENCE:genetic interaction:GO:0004103 choline kinase activity [PMID:10329685]
EXISTENCE:genetic interaction:GO:0004305 ethanolamine kinase activity [PMID:10329685]
EXISTENCE:genetic interaction:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:10329685]
EXISTENCE:genetic interaction:GO:0006656 phosphatidylcholine biosynthetic process [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0004103 choline kinase activity [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0004305 ethanolamine kinase activity [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:10329685]
Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004737 pyruvate decarboxylase activity [PMID:2185016|PMID:23423327]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:16850348]
EXISTENCE:direct assay:GO:0006090 pyruvate metabolic process [PMID:2185016]
EXISTENCE:direct assay:GO:0019655 glycolytic fermentation to ethanol [PMID:2185016]
EXISTENCE:genetic interaction:GO:0004737 pyruvate decarboxylase activity [PMID:2185016]
EXISTENCE:genetic interaction:GO:0006090 pyruvate metabolic process [PMID:2185016]
EXISTENCE:genetic interaction:GO:0019655 glycolytic fermentation to ethanol [PMID:2185016]
Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
ARS1215; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:12832395]
EXISTENCE:direct assay:GO:0017108 5'-flap endonuclease activity [PMID:12832395]
EXISTENCE:genetic interaction:GO:0000727 double-strand break repair via break-induced replication [PMID:23071463]
EXISTENCE:genetic interaction:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:18471978]
EXISTENCE:genetic interaction:GO:0006261 DNA-templated DNA replication [PMID:12832395]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:18471978]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:12228808]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:12228808]
EXISTENCE:mutant phenotype:GO:1903775 regulation of DNA double-strand break processing [PMID:26490958]
EXISTENCE:mutant phenotype:GO:1905261 regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination [PMID:27172214]
EXISTENCE:mutant phenotype:GO:2000001 regulation of DNA damage checkpoint [PMID:26490958]
EXISTENCE:physical interaction:GO:0033557 Slx1-Slx4 complex [PMID:12832395]
Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:15652485|PMID:35858543]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922|PMID:18923425]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21135132|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:35858543]
EXISTENCE:direct assay:GO:0015990 electron transport coupled proton transport [PMID:35858543]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:29912874]
EXISTENCE:direct assay:GO:0045182 translation regulator activity [PMID:35858543]
EXISTENCE:direct assay:GO:1990641 response to iron ion starvation [PMID:29912874]
EXISTENCE:direct assay:GO:2000766 negative regulation of cytoplasmic translation [PMID:35858543]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:15652485]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:15652485]
mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; represses mitochondrial function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication
S-adenosylmethionine-dependent methyltransferase
EXISTENCE:direct assay:GO:0006479 protein methylation [PMID:21460220]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:21460220]
Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15950597|PMID:9802016]
EXISTENCE:direct assay:GO:0015385 sodium:proton antiporter activity [PMID:15950597]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:19254924]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:16402204]
EXISTENCE:mutant phenotype:GO:0015491 cation:cation antiporter activity [PMID:9802016]
EXISTENCE:mutant phenotype:GO:0030004 cellular monovalent inorganic cation homeostasis [PMID:9802016]
EXISTENCE:mutant phenotype:GO:0030007 cellular potassium ion homeostasis [PMID:16402204]
EXISTENCE:mutant phenotype:GO:0036376 sodium ion export across plasma membrane [PMID:9802016]
EXISTENCE:mutant phenotype:GO:0097623 potassium ion export across plasma membrane [PMID:9802016]
Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8917313]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:8917313]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:12637560|PMID:15692048]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8917313]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:12637560]
Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
EXISTENCE:direct assay:GO:0000500 RNA polymerase I upstream activating factor complex [PMID:8846924]
EXISTENCE:direct assay:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:8846924]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:20154141]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
EXISTENCE:curator inference:GO:0006537 glutamate biosynthetic process [PMID:387737]
EXISTENCE:direct assay:GO:0004657 proline dehydrogenase activity [PMID:20450881]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:3125423|PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0010133 proline catabolic process to glutamate [PMID:20450881]
EXISTENCE:mutant phenotype:GO:0004657 proline dehydrogenase activity [PMID:387737]
EXISTENCE:mutant phenotype:GO:0010133 proline catabolic process to glutamate [PMID:387737]
Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0017178 diphthine-ammonia ligase activity [PMID:23169644]
EXISTENCE:direct assay:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:23169644]
EXISTENCE:mutant phenotype:GO:0017178 diphthine-ammonia ligase activity [PMID:23169644]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:23169644|PMID:23468660]
Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
EXISTENCE:direct assay:GO:0030036 actin cytoskeleton organization [PMID:9214384]
EXISTENCE:direct assay:GO:0042973 glucan endo-1,3-beta-D-glucosidase activity [PMID:17933563]
EXISTENCE:genetic interaction:GO:0042973 glucan endo-1,3-beta-D-glucosidase activity [PMID:12455695]
Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:15637077]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14690591]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:17881380]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15637077]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380]
Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
EXISTENCE:mutant phenotype:GO:0006597 spermine biosynthetic process [PMID:9573363]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
EXISTENCE:mutant phenotype:GO:0016768 spermine synthase activity [PMID:9573363]
Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9012791|PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10944212]
EXISTENCE:direct assay:GO:0033263 CORVET complex [PMID:17488625]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:16601699]
EXISTENCE:direct assay:GO:0035542 regulation of SNARE complex assembly [PMID:18385512]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:direct assay:GO:0099022 vesicle tethering [PMID:27307091]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:11422941]
EXISTENCE:genetic interaction:GO:0045324 late endosome to vacuole transport [PMID:11422941]
EXISTENCE:mutant phenotype:GO:0006904 vesicle docking involved in exocytosis [PMID:10978279]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:11030345]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:physical interaction:GO:0030897 HOPS complex [PMID:10944212]
Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
Substrate receptor of the glucose-induced degradation-deficient (GID) complex that targets gluconeogenic enzymes for proteasomal degradation upon switch from ethanol to glucose as a carbon source; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:15654430]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15044472|PMID:11914276]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:15044472]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:15044472]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:15044472]
EXISTENCE:direct assay:GO:0043066 negative regulation of apoptotic process [PMID:11514626]
EXISTENCE:direct assay:GO:0045142 triplex DNA binding [PMID:18029617]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:12207228]
EXISTENCE:genetic interaction:GO:0006414 translational elongation [PMID:19666721]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:19666721]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:16580682]
EXISTENCE:mutant phenotype:GO:0043558 regulation of translational initiation in response to stress [PMID:16580682]
Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:10922370]
EXISTENCE:direct assay:GO:0008413 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity [PMID:15475388]
EXISTENCE:direct assay:GO:0010945 CoA pyrophosphatase activity [PMID:10922370]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15475388]
EXISTENCE:mutant phenotype:GO:0008413 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity [PMID:15475388]
8-oxo-dGTP diphosphatase; prevents spontaneous mutagenesis via sanitization of oxidized purine nucleoside triphosphates; can also act as peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member; similar E. coli MutT and human, rat and mouse MTH1
hypothetical protein; YLR152C is not an essential gene
EXISTENCE:direct assay:GO:0003987 acetate-CoA ligase activity [PMID:8910545]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16857587|PMID:19618123]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19618123]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9711835|PMID:16857587]
EXISTENCE:direct assay:GO:0006085 acetyl-CoA biosynthetic process [PMID:8910545]
EXISTENCE:direct assay:GO:0016880 acid-ammonia (or amide) ligase activity [PMID:18305111]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:16857587]
Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
EXISTENCE:curator inference:GO:0043137 DNA replication, removal of RNA primer [PMID:14734815]
EXISTENCE:direct assay:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
EXISTENCE:direct assay:GO:0006401 RNA catabolic process [PMID:14734815]
EXISTENCE:direct assay:GO:0032299 ribonuclease H2 complex [PMID:14734815]
EXISTENCE:genetic interaction:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
ARS1216; Autonomously Replicating Sequence
35S ribosomal RNA (35S rRNA) transcript; encoded by the RDN1 locus; processed into the 25S, 18S and 5.8S rRNAs (represented by the RDN25, RDN18, and RDN58 loci)
Non-coding region located adjacent to and downstream of RDN25; transcribed as part of the 35S rRNA precursor transcript; contains the primary rRNA transcription termination site at +93 and a secondary termination site between +211 and +250
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:23673663]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:26824023]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:26824023]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:8608449]
25S ribosomal RNA (25S rRNA); component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript; formed by endonucleolytic cleavage at the C2 site of internal transcribed spacer 2 (ITS2)
EXISTENCE:genetic interaction:GO:0043457 regulation of cellular respiration [PMID:18622616]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:12414727]
Protein potentially involved in regulation of respiratory metabolism; located in the mitochondria; interacts genetically with RPO41 and physically with Coq5p; encoded within the 25S rRNA gene on the opposite strand
Non-coding region between RDN58 and RDN25; transcribed as part of the 35S rRNA precursor transcript; forms a stem-loop structure that is required for processing of the precursor transcript
5.8S ribosomal RNA (5.8S rRNA); component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript; 3' end formation involves processing by the exosome complex while 5' end is generated by Kem1p and Rat1p
Non-coding region between RDN58 and RDN18; transcribed as part of the 35S rRNA precursor transcript; excision during transcript maturation is coupled with processing of the 3' external transcribed spacer (3'ETS)
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:11701127]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:20699223|PMID:18708582]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:18003936]
EXISTENCE:mutant phenotype:GO:0002188 translation reinitiation [PMID:18708582]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:19029250]
EXISTENCE:mutant phenotype:GO:0061770 translation elongation factor binding [PMID:19029250]
18S ribosomal RNA (18S rRNA); component of the small (40S) ribosomal subunit; involved in codon recognition by tRNAs or the release factor eRF1 (Sup45p); encoded in the rDNA repeat (RDN1) as part of the 35S primary transcript
Non-coding region located immediately upstream of RDN18; transcribed as part of the 35S rRNA precursor transcript; contains an essential U3 snoRNA binding site required for maturation of 18S rRNA
NTS2-1; Non-transcribed region of the rDNA repeat; located between RND5 and the 5'ETS; contains RNA polymerase I termination site
ARS1200-1; Chromosomal origin of bidirectional DNA replication; an rDNA ARS is presumed to be present in each tandem rDNA repeat, but fewer than one-third of the rDNA ARSs are actually used as replication origins in a given S phase
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:16047201]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:16047201]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:16047201]
5S ribosomal RNA (5S rRNA); only complete sequence of 6 repeated RDN5 alleles; able to support viability when provided as sole form of 5S rRNA; component of the large (60S) ribosomal subunit; localized to the nucleolus via interaction with Rpl5p; may play a role in translational frame fidelity; transcription is mediated by PolIII and activated by TFIIIA and TFIIIE
NTS1-2; Non-transcribed region of the rDNA repeat between the 3' ETS and RDN5; required for rDNA repeat expansion
Non-coding region located adjacent to RDN25; transcribed as part of the 35S rRNA precursor transcript; contains the primary rRNA transcription termination site at +93 and a secondary termination site between +211 and +250
hypothetical protein; identified by fungal homology comparisons and RT-PCR; this ORF is contained within RDN25-2 and RDN37-2
Non-coding region between RDN58 and RDN25; transcribed as part of the 35S rRNA precursor transcript; forms a stem-loop structure required for processing of the precursor transcript
5.8S ribosomal RNA (5.8S rRNA); component of the large (60S) ribosomal subunit; encoded in the rDNA repeat (RDN1)
Non-coding region between RDN58 and RDN18; transcribed as part of the 35S rRNA precursor transcript; excision during transcript maturation is coupled with processing of the 3' external transcribed spacer (3' ETS)
NTS2-2; Non-transcribed region of the rDNA repeat; located between RDN5 and the 5' ETS
ARS1200-2; Chromosomal origin of bidirectional DNA replication; an rDNA ARS is presumed to be present in each tandem rDNA repeat, but fewer than one-third of the rDNA ARSs are actually used as replication origins in a given S phase
Variant 5S ribosomal rRNA (5S rRNA) gene; located within the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-3, RDN5-4, and RDN5-5
hypothetical protein; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-2; YLR154C-H has a paralog, YLR157C-C, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0004067 asparaginase activity [PMID:238936]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11914276]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0006530 asparagine catabolic process [PMID:3042786]
EXISTENCE:direct assay:GO:0006995 cellular response to nitrogen starvation [PMID:3042786]
EXISTENCE:direct assay:GO:0030287 cell wall-bounded periplasmic space [PMID:238936]
Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from wine yeast Wickerhamomyces anomalus or close relative; enzyme preparation shows antitumor activity; reference strain S288C has four copies of ASP3; ASP3-1 has paralog ASP3-3 that arose from a segmental duplication
hypothetical protein; exhibits a two-hybrid interaction with Jsn1p in a large-scale analysis; YLR156W has a paralog, YLR159W, that arose from a segmental duplication
Variant 5S ribosomal RNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-4, and RDN5-5
hypothetical protein; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-3; YLR156C-A has a paralog, YLR159C-A, that arose from a segmental duplication
Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; enzyme preparation shows antitumor activity; reference strain S288C has four copies of ASP3; ASP3-2 has a paralog, ASP3-4, that arose from a segmental duplication
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YLR157W-D has a paralog, YLR161W, that arose from a segmental duplication
YLRCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
hypothetical protein identified by gene-trapping; microarray-based expression analysis, and genome-wide homology searching; partially overlaps a Ty1 element
Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-3, and RDN5-5
hypothetical protein; identified by fungal homology comparisons and RT-PCR; this ORF partially overlaps RDN5-4; YLR157C-C has a paralog, YLR154C-H, that arose from a segmental duplication
Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; enzyme preparation shows antitumor activity; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication
hypothetical protein; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR159W has a paralog, YLR156W, that arose from a segmental duplication
Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus; unable to support cell viability when provided as sole source of 5S rRNA; identical in sequence to variant alleles RDN5-2, RDN5-3, and RDN5-4
hypothetical protein; identified by fungal homology comparisons and RT-PCR; partially overlaps RND5-5; YLR159C-A has a paralog, YLR156C-A, that arose from a segmental duplication
Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; enzyme preparation shows antitumor activity; reference strain S288C has four copies of ASP3; ASP3-4 has a paralog, ASP3-2, that arose from a segmental duplication
hypothetical protein; YLR156W, YLR159W, and YLR161W are three identical open reading frames in S288C encoded near the ribosomal DNA region of chromosome 12; YLR161W has a paralog, YLR157W-D, that arose from a segmental duplication
Variant 5S ribosomal rRNA (5S rRNA) gene; located at a site distal to the RDN1 locus
hypothetical protein; overexpression confers resistance to the antimicrobial peptide MiAMP1 and causes growth arrest, apoptosis, and increased sensitivity to cobalt chloride
hypothetical protein; identified by fungal homology comparisons and RT-PCR; identified in a screen for mutants with decreased levels of rDNA transcription
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:2905264]
EXISTENCE:direct assay:GO:0006627 protein processing involved in protein targeting to mitochondrion [PMID:2007593]
EXISTENCE:direct assay:GO:0017087 mitochondrial processing peptidase complex [PMID:2905264]
Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10637294]
EXISTENCE:genetic interaction:GO:0005749 mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) [PMID:25328978]
EXISTENCE:genetic interaction:GO:0006121 mitochondrial electron transport, succinate to ubiquinone [PMID:25328978]
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:25328978]
Putative alternate subunit of succinate dehydrogenase (SDH); mitochondrial inner membrane protein; genetic interaction with SDH4 suggests that Shh4p can function as a functional SDH subunit; a fraction copurifies with SDH subunit Sdh3p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Shh4p has greater similarity to human SDHD (subunit D of SDH, implicated in paraganglioma) than does its paralog Sdh4p
EXISTENCE:mutant phenotype:GO:0000154 rRNA modification [PMID:10756195]
EXISTENCE:mutant phenotype:GO:0001522 pseudouridine synthesis [PMID:10756195]
EXISTENCE:mutant phenotype:GO:0004730 pseudouridylate synthase activity [PMID:10756195]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:10756195]
Pseudouridine synthase; catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:8978675]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
ubiquitin-ribosomal 40S subunit protein S31 fusion protein
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:18782943|PMID:385049]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:18782943|PMID:385049]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:385049|PMID:18782943]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:9830035]
EXISTENCE:mutant phenotype:GO:0002109 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) [PMID:2538753]
EXISTENCE:mutant phenotype:GO:0031386 protein tag [PMID:2538753]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:16246728]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:32408794]
Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19506038]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:27354379]
EXISTENCE:genetic interaction:GO:0032048 cardiolipin metabolic process [PMID:19506038]
EXISTENCE:genetic interaction:GO:0042407 cristae formation [PMID:19221197]
EXISTENCE:genetic interaction:GO:0046337 phosphatidylethanolamine metabolic process [PMID:19221197]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:19221197]
EXISTENCE:mutant phenotype:GO:0046337 phosphatidylethanolamine metabolic process [PMID:19221197]
EXISTENCE:mutant phenotype:GO:2001246 negative regulation of phosphatidylcholine biosynthetic process [PMID:22403410]
Mitochondrial intermembrane space protein; involved in phospholipid metabolism; forms complex with Mdm35p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI; UPS2 has a paralog, UPS3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0030121 AP-1 adaptor complex [PMID:10564262]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:17003107]
Small subunit of the clathrin-associated adaptor complex AP-1; AP-1 is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
EXISTENCE:direct assay:GO:0004164 diphthine synthase activity [PMID:3042777]
EXISTENCE:direct assay:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:3042777]
EXISTENCE:mutant phenotype:GO:0004164 diphthine synthase activity [PMID:1508200]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:1508200]
Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347|PMID:26928762|PMID:33536246]
EXISTENCE:mutant phenotype:GO:0006995 cellular response to nitrogen starvation [PMID:33536246]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:33536246]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:33536246]
Vacuolar membrane protein involved in termination of autophagy; regulates autophagy termination during persistent nitrogen starvation through Atg1p-mediated re-phosphorylation of Atg13p and PAS disassembly; type II membrane protein containing hydrophobic transmembrane region with N-terminal region oriented toward cytosol and C-terminus inside lumen; luminal domain is glycosylated; forms foci in vacuole membrane during starvation; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0004450 isocitrate dehydrogenase (NADP+) activity [PMID:15574419]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15001388|PMID:19854152]
EXISTENCE:genetic interaction:GO:0006102 isocitrate metabolic process [PMID:15574419]
EXISTENCE:mutant phenotype:GO:0006102 isocitrate metabolic process [PMID:15574419]
Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication; mutation in human homolog IDH1 is associated with low-grade gliomas and secondary glioblastomas
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9472021]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:15962000]
EXISTENCE:direct assay:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:mutant phenotype:GO:0000495 box H/ACA RNA 3'-end processing [PMID:15964797]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9472021]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:9472021]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9472021]
EXISTENCE:mutant phenotype:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25192136|PMID:25219674]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:9472021]
Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on large and small rRNAs, on snRNA U2, and on some mRNAs; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9741624]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9741624]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:11914276]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16835445]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16835445]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16107689]
Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
hypothetical protein; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
ARS1217; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:17317927]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:17317927]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:17076703]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17317927]
EXISTENCE:direct assay:GO:0030162 regulation of proteolysis [PMID:9521655]
EXISTENCE:direct assay:GO:0030414 peptidase inhibitor activity [PMID:9521655]
EXISTENCE:mutant phenotype:GO:0046578 regulation of Ras protein signal transduction [PMID:15075275]
EXISTENCE:physical interaction:GO:0046578 regulation of Ras protein signal transduction [PMID:15075275]
Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17317927|PMID:14562095|PMID:11914276]
hypothetical protein with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:genetic interaction:GO:0004478 methionine adenosyltransferase activity [PMID:3316224]
EXISTENCE:mutant phenotype:GO:0006555 methionine metabolic process [PMID:4580557]
EXISTENCE:mutant phenotype:GO:0006556 S-adenosylmethionine biosynthetic process [PMID:17426150]
EXISTENCE:mutant phenotype:GO:0062030 negative regulation of stress granule assembly [PMID:32609300]
S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:16505166|PMID:16601096]
EXISTENCE:direct assay:GO:0005771 multivesicular body [PMID:14701806]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:16505166]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:16505166]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:16505166]
EXISTENCE:mutant phenotype:GO:0001671 ATPase activator activity [PMID:16505166]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:12953057]
Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:9719633]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12697814|PMID:8590795|PMID:24470217]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12697814|PMID:8590795]
EXISTENCE:direct assay:GO:0030907 MBF transcription complex [PMID:8372350]
EXISTENCE:direct assay:GO:0033309 SBF transcription complex [PMID:1832338|PMID:8423776]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9719633]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:9719633]
EXISTENCE:mutant phenotype:GO:0010845 positive regulation of reciprocal meiotic recombination [PMID:9628912]
EXISTENCE:mutant phenotype:GO:0033309 SBF transcription complex [PMID:8423776]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:20641022]
EXISTENCE:mutant phenotype:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:2649246|PMID:3542227]
EXISTENCE:physical interaction:GO:0033309 SBF transcription complex [PMID:1465410]
Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12464632]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:12760057|PMID:22333912]
Putative transcription factor, contains Forkhead Associated domain; binds chromatin; involved in expression homeostasis, buffering of mRNA synthesis rate against gene dosage changes during S phase; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase complexes; APCC(Cdh1) substrate; relative distribution to nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23268442]
EXISTENCE:genetic interaction:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23268442]
Ribosomal 60S subunit protein L37A; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
18S rRNA pseudouridine methyltransferase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11694595|PMID:11935223]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11694595|PMID:11914276]
EXISTENCE:direct assay:GO:0005880 nuclear microtubule [PMID:11935223]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:22588997|PMID:12150911]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:16721597]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11694595]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:11694595]
EXISTENCE:mutant phenotype:GO:0070037 rRNA (pseudouridine) methyltransferase activity [PMID:20972225]
EXISTENCE:mutant phenotype:GO:0070475 rRNA base methylation [PMID:20972225]
Methyltransferase for rRNA; methylates pseudouridine 18S rRNA residue 1191; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independent of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and equivalent yeast mutation affects Emg1p dimerization and localization but not methyltransferase activity; human EMG1 complements lethality of null and ts mutant
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:22842922]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11251115]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12746444]
EXISTENCE:mutant phenotype:GO:0015421 ABC-type oligopeptide transporter activity [PMID:11251115]
EXISTENCE:mutant phenotype:GO:0090374 oligopeptide export from mitochondrion [PMID:11251115]
Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress
EXISTENCE:direct assay:GO:0016906 sterol 3-beta-glucosyltransferase activity [PMID:10224056]
EXISTENCE:genetic interaction:GO:0032120 ascospore-type prospore membrane formation [PMID:18756268]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:10224056]
UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:13679573]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:30044712]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:15201867|PMID:16407407]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:13679573]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:13679573]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:22119524]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:18245340]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:15201867]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:15201867|PMID:18245340]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:35281729|PMID:15201867|PMID:22119524]
Phospholipid binding protein; interacts with mitochondria and with Myo2p, functioning as an adaptor that recruits Myo2p and facilitates actin-based transport of mitochondria to the bud; mediates mitochondria anchorage at the bud tip; phosphorylated protein that localizes to the mitochondrial outer membrane; mRNA is targeted to the bud via the transport system involving She2p; member of the DSL1 family of tethering proteins
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:8858167|PMID:8858166]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:12667447|PMID:22375831]
EXISTENCE:genetic interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:10087260]
EXISTENCE:physical interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:10087260|PMID:12595255|PMID:15798189]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:10087260|PMID:12595255]
Peroxisomal importomer complex component; integral peroxisomal membrane protein required for docking and translocation of peroxisomal matrix proteins; interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p; forms a complex with Pex14p and Pex17p; human homolog PEX13 complements yeast null mutant
translation initiation factor eIF3 core subunit j
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11560931]
EXISTENCE:physical interaction:GO:0002181 cytoplasmic translation [PMID:11179233]
EXISTENCE:physical interaction:GO:0003743 translation initiation factor activity [PMID:11179233]
EXISTENCE:physical interaction:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:11179233]
eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for 20S pre-rRNA processing; required at post-transcriptional step for efficient retrotransposition; absence decreases Ty1 Gag:GFP protein levels; binds eIF3 subunits Rpg1p, Prt1p and 18S rRNA; eIF3 also involved in programmed stop codon read through; human homolog EIF3J can complement yeast hcr1 mutant
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:26235513]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:16754953|PMID:19506038]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:23042293]
EXISTENCE:direct assay:GO:0045332 phospholipid translocation [PMID:26235513]
EXISTENCE:direct assay:GO:1990050 phosphatidic acid transfer activity [PMID:23042293]
EXISTENCE:mutant phenotype:GO:0015914 phospholipid transport [PMID:23042293]
EXISTENCE:mutant phenotype:GO:0032048 cardiolipin metabolic process [PMID:19221197|PMID:19506038]
EXISTENCE:mutant phenotype:GO:1990050 phosphatidic acid transfer activity [PMID:23042293]
EXISTENCE:mutant phenotype:GO:2001247 positive regulation of phosphatidylcholine biosynthetic process [PMID:22403410]
Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:11016834]
EXISTENCE:mutant phenotype:GO:0006033 chitin localization [PMID:33945065]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:27246500]
GPI-anchored protein involved in cell wall remodeling; involved in the assembly of the cell wall chitin-glucan structure and repair of damage caused by the antifungal agent polyhexamethylene biguanide; role in the cell wall integrity pathway; expression is upregulated in response to cell wall stress; transported to the cell surface after Pmt1p-Pmt2p-mediated O-mannosylation, colocalizing with bud scars
glycylpeptide N-tetradecanoyltransferase NMT1
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:1544917]
EXISTENCE:mutant phenotype:GO:0004379 glycylpeptide N-tetradecanoyltransferase activity [PMID:8955162]
N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:15588312]
Protein with WD-40 repeats involved in rRNA processing; associates with rDNA chromatin in H4-tail-dependent manner; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9372940|PMID:11914276]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:11081632]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:10733567]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:17431397]
Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
tubulin-binding prefolding complex subunit YKE2
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:24068951]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9463374]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:9463374]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:physical interaction:GO:0016272 prefoldin complex [PMID:9463374]
Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:17391640|PMID:16027161]
EXISTENCE:mutant phenotype:GO:1901006 ubiquinone-6 biosynthetic process [PMID:16027161]
EXISTENCE:physical interaction:GO:0006744 ubiquinone biosynthetic process [PMID:17391640]
Protein required for ubiquinone biosynthesis and respiratory growth; localizes to matrix face of mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; ubiquinone is also known as coenzyme Q; human homolog COQ9 can complement yeast coq9 null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:genetic interaction:GO:0070131 positive regulation of mitochondrial translation [PMID:15306853]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:15306853]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:19710419]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:15306853|PMID:19710419]
EXISTENCE:physical interaction:GO:0070131 positive regulation of mitochondrial translation [PMID:15306853]
Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:28154081]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:16339141]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:28154081]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:16339141]
EXISTENCE:mutant phenotype:GO:0061668 mitochondrial ribosome assembly [PMID:30968120]
EXISTENCE:mutant phenotype:GO:0070124 mitochondrial translational initiation [PMID:30968120]
Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
EXISTENCE:direct assay:GO:0004392 heme oxygenase (decyclizing) activity [PMID:17144670]
EXISTENCE:direct assay:GO:0005640 nuclear outer membrane [PMID:21081499]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12840010]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11566881]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:12840010]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:21081499]
EXISTENCE:mutant phenotype:GO:0042167 heme catabolic process [PMID:12840010]
ER localized heme oxygenase; involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:direct assay:GO:0070530 K63-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:10449404]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10449404]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:10449404]
Epsin-like protein required for endocytosis and actin patch assembly; K48-, K63-specific Ub chain binding protein that functions as an endocytic adaptor; contains clathrin-binding motif at C-terminus; functionally redundant with Ent1p; ENT2 has a paralog, ENT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000838 Hrd1p ubiquitin ligase ERAD-M complex [PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:11390656]
EXISTENCE:direct assay:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:12105183]
EXISTENCE:direct assay:GO:0034099 luminal surveillance complex [PMID:16873065]
EXISTENCE:direct assay:GO:1905524 negative regulation of protein autoubiquitination [PMID:31713515]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:24292014]
EXISTENCE:physical interaction:GO:0005789 endoplasmic reticulum membrane [PMID:16619026]
ER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12671686|PMID:17604721]
EXISTENCE:direct assay:GO:0030127 COPII vesicle coat [PMID:11717432|PMID:8004676]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:11823431|PMID:18046406]
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:11389436]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:22300850]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:12538638]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:10747086]
EXISTENCE:mutant phenotype:GO:0090114 COPII-coated vesicle budding [PMID:8223424|PMID:8432727]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:23974112]
Structural component of 3 complexes; subunit of the Nup84p nuclear pore subcomplex that contributes to nucleocytoplasmic transport and NPC biogenesis; subunit of the COPII vesicle coat required for ER-to-Golgi transport; subunit of SEACAT, a subcomplex of the coatomer-related, vacuolar-associated SEA complex, that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p, thereby resulting in activation of TORC1 signaling; human SEC13 homolog
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:17482543]
EXISTENCE:direct assay:GO:0004731 purine-nucleoside phosphorylase activity [PMID:11466296]
EXISTENCE:direct assay:GO:0047724 inosine nucleosidase activity [PMID:19001417]
EXISTENCE:direct assay:GO:0070635 nicotinamide riboside hydrolase activity [PMID:19001417]
EXISTENCE:genetic interaction:GO:0019358 nicotinate nucleotide salvage [PMID:17482543]
EXISTENCE:genetic interaction:GO:0034356 NAD biosynthesis via nicotinamide riboside salvage pathway [PMID:19001417]
EXISTENCE:mutant phenotype:GO:0004731 purine-nucleoside phosphorylase activity [PMID:11466296]
EXISTENCE:mutant phenotype:GO:0006148 inosine catabolic process [PMID:11466296|PMID:23670538]
EXISTENCE:mutant phenotype:GO:0046115 guanosine catabolic process [PMID:11466296|PMID:23670538]
Purine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12972503|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12972503]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:14562095]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:11438663|PMID:1387566|PMID:16688214]
EXISTENCE:genetic interaction:GO:1901673 regulation of mitotic spindle assembly [PMID:8319908]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:8455600]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:1427070]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:8455600]
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:24165940]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:24165940]
EXISTENCE:direct assay:GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I [PMID:24165940]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:24165940]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:24165940]
Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:24390141]
EXISTENCE:direct assay:GO:0005822 inner plaque of spindle pole body [PMID:9215630]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:9215630]
EXISTENCE:direct assay:GO:0007020 microtubule nucleation [PMID:11950928|PMID:24842996]
EXISTENCE:direct assay:GO:0008275 gamma-tubulin small complex [PMID:9130700|PMID:9384578|PMID:25599398]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:8557744]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:9153752]
EXISTENCE:mutant phenotype:GO:0051417 microtubule nucleation by spindle pole body [PMID:8707828]
EXISTENCE:mutant phenotype:GO:2000767 positive regulation of cytoplasmic translation [PMID:26310304]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9130700|PMID:8670895]
EXISTENCE:physical interaction:GO:0008275 gamma-tubulin small complex [PMID:9130700]
Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress; null mutation is complemented by human TUBG1
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:15470107]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15470107]
Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
EXISTENCE:direct assay:GO:0000293 ferric-chelate reductase activity [PMID:8164662]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8164662|PMID:17507646]
EXISTENCE:direct assay:GO:0006826 iron ion transport [PMID:8164662]
EXISTENCE:direct assay:GO:0015677 copper ion import [PMID:7814363]
Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels
EXISTENCE:mutant phenotype:GO:0045948 positive regulation of translational initiation [PMID:23775072]
EXISTENCE:mutant phenotype:GO:1905143 eukaryotic translation initiation factor 2 complex assembly [PMID:23775072|PMID:26211610]
Assembly factor for the eIF2 translation initiation factor complex; regulates translational initiation; conserved residues of this ATP-Grasp protein that bind to ATP-Mg2+ in the pombe ortholog are required for complex assembly in budding yeast; interaction with eIF2 subunit Gcd11p facilitates complex assembly and activity; required for the START transition and timely progression through G2; regulated by nutrient availability; human ortholog complements the yeast mutant
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:10942767|PMID:9191025]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9191025|PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:9927435]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:10942767]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:25380751]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10942767]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:9927435]
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19703468|PMID:20624914]
EXISTENCE:genetic interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:20624914|PMID:23198688]
EXISTENCE:mutant phenotype:GO:0033108 mitochondrial respiratory chain complex assembly [PMID:19703468]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:20624914|PMID:23198688]
Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors; mutation can be complemented by human COA4
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511544]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:34203091]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11390404|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8866476]
EXISTENCE:mutant phenotype:GO:0005384 manganese ion transmembrane transporter activity [PMID:11390404]
EXISTENCE:mutant phenotype:GO:0006826 iron ion transport [PMID:11390404]
EXISTENCE:mutant phenotype:GO:0006828 manganese ion transport [PMID:11390404]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:7941738]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:34203091|PMID:10713071]
EXISTENCE:mutant phenotype:GO:0015093 ferrous iron transmembrane transporter activity [PMID:11390404|PMID:34203091]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:8866476]
EXISTENCE:mutant phenotype:GO:1990461 detoxification of iron ion [PMID:34203091]
Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to vacuole decreases upon DNA replication stress; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:15126390]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:15126390]
Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:15620355]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15466158|PMID:14562095]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15466158]
EXISTENCE:direct assay:GO:0032545 CURI complex [PMID:17452446]
EXISTENCE:genetic interaction:GO:0003713 transcription coactivator activity [PMID:15620355]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15620355]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:9755194]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15616569]
Coactivator, regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing; regulated by acetylation and phosphorylation at different growth states via TORC1 signaling; IFH1 has a paralog, CRF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
EXISTENCE:direct assay:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:25982115]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:25982115]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
F-box protein and component of SCF ubiquitin ligase complexes; involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; SCF-Ucc1 regulates level of Cit2 citrate synthase protein to maintain citrate homeostasis, acts as metabolic switch for glyoxylate cycle; UCC1 transcription is downregulated in cells grown on C2-compounds
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:10982824]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:10982824]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23109428]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:10982824]
EXISTENCE:mutant phenotype:GO:0033184 positive regulation of histone ubiquitination [PMID:16040246]
EXISTENCE:mutant phenotype:GO:0051571 positive regulation of histone H3-K4 methylation [PMID:16040246]
EXISTENCE:mutant phenotype:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19328068]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:10982824]
Cyclin for the Sgv1p (Bur1p) protein kinase; subunit of the CDK-cyclin BUR kinase complex with Sgv1p (Bur1p); regulates Set2p-dependent H3K36 trimethylation; regulates transcriptional elongation through the phosphorylation of the carboxy-terminal domain (CTD) of Rpo21p; involved in the recruitment of Spt6p to the CTD at the onset of transcription; regulates telomere elongation
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:direct assay:GO:0035974 meiotic spindle pole body [PMID:12796288]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:mutant phenotype:GO:0035974 meiotic spindle pole body [PMID:12796288]
Structural component of the meiotic outer plaque; outer plaque is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
YLRWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:11533229]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11533229]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11533229]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18675371]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:18675371]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11208779|PMID:26448198]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:16055745]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11533229|PMID:16055745|PMID:26448198]
Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
ARS1218; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12455994|PMID:8167411]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12455994|PMID:11598008|PMID:26928762]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11591390]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:32556066]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12455994|PMID:8167411]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12455994|PMID:8167411]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12455994]
EXISTENCE:direct assay:GO:0005940 septin ring [PMID:23906065]
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:12455994]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:22832273|PMID:8167411]
EXISTENCE:direct assay:GO:0071763 nuclear membrane organization [PMID:32556066]
EXISTENCE:genetic interaction:GO:0045921 positive regulation of exocytosis [PMID:19955214]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:22323294]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:11003652]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:10066831]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:10066831]
EXISTENCE:mutant phenotype:GO:0007119 budding cell isotropic bud growth [PMID:10066831]
EXISTENCE:mutant phenotype:GO:0007266 Rho protein signal transduction [PMID:1904541]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:23468594]
EXISTENCE:mutant phenotype:GO:0031106 septin ring organization [PMID:14517318|PMID:16371506]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:11598008]
EXISTENCE:mutant phenotype:GO:0045921 positive regulation of exocytosis [PMID:11595741]
EXISTENCE:mutant phenotype:GO:0060178 regulation of exocyst localization [PMID:11595741]
EXISTENCE:mutant phenotype:GO:2000222 positive regulation of pseudohyphal growth [PMID:8643578]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9367980|PMID:9367979]
EXISTENCE:physical interaction:GO:0045921 positive regulation of exocytosis [PMID:19955214]
Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; acts with ESCRT proteins at sites of nuclear envelope and ER fission; mutants have defects in the organization of actin and septins; human homolog CDC42 can complement yeast cdc42 mutant
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
EXISTENCE:genetic interaction:GO:0034354 'de novo' NAD biosynthetic process from tryptophan [PMID:12062417]
EXISTENCE:mutant phenotype:GO:0030429 kynureninase activity [PMID:12062417]
Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:8985179]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12101098]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:10898986]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12101098]
EXISTENCE:direct assay:GO:0007004 telomere maintenance via telomerase [PMID:10898986]
EXISTENCE:direct assay:GO:0032210 regulation of telomere maintenance via telomerase [PMID:27659693]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:17656141]
EXISTENCE:direct assay:GO:0071919 G-quadruplex DNA formation [PMID:20098422|PMID:23376615]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:2655926]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:10898986|PMID:8978029]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:12414733]
TLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:1324925]
EXISTENCE:direct assay:GO:0003916 DNA topoisomerase activity [PMID:22885009]
EXISTENCE:direct assay:GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity [PMID:1324925]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15899853]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:16546998]
EXISTENCE:mutant phenotype:GO:0000018 regulation of DNA recombination [PMID:1328869]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:7708702]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:17571075]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:1328869]
EXISTENCE:physical interaction:GO:0031422 RecQ family helicase-topoisomerase III complex [PMID:15889139]
DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination
hypothetical protein; conserved across S. cerevisiae strains; overlaps ORF YLR235C
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16850348|PMID:9235906]
EXISTENCE:genetic interaction:GO:0015234 thiamine transmembrane transporter activity [PMID:9358046]
EXISTENCE:genetic interaction:GO:0015888 thiamine transport [PMID:16850348|PMID:9358046]
EXISTENCE:genetic interaction:GO:1903088 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transport [PMID:24778186]
EXISTENCE:genetic interaction:GO:1903089 5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity [PMID:24778186]
EXISTENCE:mutant phenotype:GO:0015234 thiamine transmembrane transporter activity [PMID:9235906|PMID:9358046]
EXISTENCE:mutant phenotype:GO:0015888 thiamine transport [PMID:16850348|PMID:9235906|PMID:9358046]
Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12514182|PMID:23625923]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; FAR10 has a paralog, VPS64, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0009249 protein lipoylation [PMID:11356580|PMID:19570983]
EXISTENCE:mutant phenotype:GO:0016874 ligase activity [PMID:11356580]
Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16421251|PMID:19001347]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:16421251]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:7989323]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:16421251]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:21121900]
EXISTENCE:direct assay:GO:0016303 1-phosphatidylinositol-3-kinase activity [PMID:8385367|PMID:7989323]
EXISTENCE:direct assay:GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I [PMID:11157979]
EXISTENCE:direct assay:GO:0034272 phosphatidylinositol 3-kinase complex, class III, type II [PMID:11157979]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:8385367|PMID:7989323]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:23335340]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:32788210]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:18586673]
EXISTENCE:mutant phenotype:GO:0016303 1-phosphatidylinositol-3-kinase activity [PMID:8385367]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:21121900]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:23335340]
EXISTENCE:mutant phenotype:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:8385367]
EXISTENCE:mutant phenotype:GO:2001159 regulation of protein localization by the Cvt pathway [PMID:18586673]
Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
EXISTENCE:direct assay:GO:0005227 calcium activated cation channel activity [PMID:24503647]
EXISTENCE:direct assay:GO:0006812 cation transport [PMID:24503647]
Calcium permeable gated cation channel; functions as an osmosensitive, calcium-permeable cation channel when expressed in a heterologous system; may be involved in detoxification; similar to Arabidopsis CSC1
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12145310|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12145310]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:23668914]
EXISTENCE:genetic interaction:GO:0035376 sterol import [PMID:11063737]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:18287539]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:18287539]
EXISTENCE:mutant phenotype:GO:0032366 intracellular sterol transport [PMID:15173322]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:11063737]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:18287539]
EXISTENCE:mutant phenotype:GO:0097036 regulation of plasma membrane sterol distribution [PMID:23668914]
Cortical ER protein; implicated in the membrane insertion of tail-anchored C-terminal single transmembrane domain proteins; may function in transport of glycosylphosphatidylinositol intermediates into the ER lumen; required for normal intracellular sterol distribution; human ARV1, required for normal cholesterol and bile acid homeostasis, can complement yeast arv1 null mutant; human variant causing early onset epileptic encephalopathy is unable to rescue the yeast null
ARS1219; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
putative signal sequence-binding GTPase GPN3
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:23267056]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:23324351]
Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; may have a role in sister chromatid cohesion; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:11968008]
EXISTENCE:direct assay:GO:0070006 metalloaminopeptidase activity [PMID:2246265]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:21124907]
EXISTENCE:mutant phenotype:GO:0035551 protein initiator methionine removal involved in protein maturation [PMID:11811952]
Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p; apo-Map1p is activated by the GTP-dependent zinc transferase Zng1p, especially during zinc starvation; interacts with Zng1p via an N-terminal Zn finger domain
EXISTENCE:direct assay:GO:0004126 cytidine deaminase activity [PMID:10501935]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11292850|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11292850|PMID:14562095]
EXISTENCE:direct assay:GO:0006216 cytidine catabolic process [PMID:10501935]
EXISTENCE:direct assay:GO:0006217 deoxycytidine catabolic process [PMID:10501935]
EXISTENCE:direct assay:GO:0008655 pyrimidine-containing compound salvage [PMID:10501935]
Cytidine deaminase; catalyzes the modification of cytidine to uridine in vitro but native RNA substrates have not been identified, localizes to both the nucleus and cytoplasm
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10490616]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:12193598]
EXISTENCE:direct assay:GO:0018345 protein palmitoylation [PMID:12193598]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:10490616]
EXISTENCE:mutant phenotype:GO:0016409 palmitoyltransferase activity [PMID:12193598]
EXISTENCE:mutant phenotype:GO:0018345 protein palmitoylation [PMID:10490616|PMID:16751107]
EXISTENCE:physical interaction:GO:0031211 endoplasmic reticulum palmitoyltransferase complex [PMID:12193598]
Subunit of a palmitoyltransferase; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; palmitoyltransferase is composed of Erf2p and Shr5p
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:24155900]
EXISTENCE:genetic interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:22367032]
EXISTENCE:genetic interaction:GO:0045003 double-strand break repair via synthesis-dependent strand annealing [PMID:22367032]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:24155900]
EXISTENCE:mutant phenotype:GO:0045003 double-strand break repair via synthesis-dependent strand annealing [PMID:22367032]
E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent NHEJ pathway; controls 2-micron plasmid copy number by regulating the levels of the Flp1p recombinase; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; null mutant displays increased levels of spontaneous Rad52p foci
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11344302]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8939941|PMID:14562095]
EXISTENCE:genetic interaction:GO:0007231 osmosensory signaling pathway [PMID:11344302]
EXISTENCE:mutant phenotype:GO:0007231 osmosensory signaling pathway [PMID:11344302]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:16381815]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:10778743]
EXISTENCE:physical interaction:GO:0007231 osmosensory signaling pathway [PMID:11344302]
Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003746 translation elongation factor activity [PMID:2670939]
EXISTENCE:direct assay:GO:0005840 ribosome [PMID:9553076]
EXISTENCE:direct assay:GO:0006415 translational termination [PMID:20534490]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405|PMID:23451152]
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:19666721]
EXISTENCE:direct assay:GO:0042788 polysomal ribosome [PMID:34916334]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:34916334]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:19666721|PMID:34916334]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:16954224]
Translation elongation factor 3; contains two ABC cassettes; binds and hydrolyzes ATP; interacts with mRNA independently of translation; involved in frameshifting at stalled ribosomes; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
Protein packaged into COPII vesicles for ER to Golgi trafficking; forms a complex with Emp47p that may function in trafficking plasma membrane glycoproteins through early secretory pathway; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15189984|PMID:23045398]
EXISTENCE:mutant phenotype:GO:0006067 ethanol metabolic process [PMID:15189984]
Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17; human homolog MPV17 is implicated in hepatocerebral mtDNA depletion syndromes (MDDS), and complements yeast null mutant
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:23781023]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:23781023]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:23781023]
EXISTENCE:genetic interaction:GO:0055088 lipid homeostasis [PMID:23781023]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:23781023]
Mitochondrial protein involved in lipid homeostasis; involved in mobilization of CoQ from its site of synthesis on the inner mitochondrial membrane to other sites of action; associates with mitochondrial ribosome; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of ERMES complex; transcription is periodic during metabolic cycle; homologous to human aarF domain containing kinase, ADCK1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15965467|PMID:14562095]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:15965467]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:15965467]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:15965467]
EXISTENCE:physical interaction:GO:0030473 nuclear migration along microtubule [PMID:15965467]
Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends; localizes to cytosol, nucleus and peroxisomes
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:3030565|PMID:9118994]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:2823123]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25378625|PMID:8182072]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25378625]
EXISTENCE:genetic interaction:GO:0043457 regulation of cellular respiration [PMID:2851658]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:17785431]
EXISTENCE:mutant phenotype:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:2823123]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:2823123]
EXISTENCE:mutant phenotype:GO:0043457 regulation of cellular respiration [PMID:2182199]
EXISTENCE:mutant phenotype:GO:0071169 establishment of protein localization to chromatin [PMID:17785431]
Zinc finger transcription factor; involved in the complex regulation of gene expression in response to levels of heme and oxygen; localizes to the mitochondrion as well as to the nucleus; the S288C sequence differs from other strain backgrounds due to a Ty1 insertion in the carboxy terminus
YLRWTy1-3; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
hypothetical protein; protein abundance increases in response to DNA replication stress
ARS1220; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004373 glycogen (starch) synthase activity [PMID:21835915]
EXISTENCE:mutant phenotype:GO:0004373 glycogen (starch) synthase activity [PMID:1908457]
EXISTENCE:mutant phenotype:GO:0005978 glycogen biosynthetic process [PMID:1908457]
Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:10869431]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:10869431]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:7902576|PMID:9256426]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9256426]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431|PMID:14597775]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:14597775]
EXISTENCE:mutant phenotype:GO:0006458 'de novo' protein folding [PMID:1978929]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:1359644]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:1347713]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:1359644]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:1359644]
EXISTENCE:mutant phenotype:GO:0051131 chaperone-mediated protein complex assembly [PMID:2645524]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:8097278]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:9256426]
Tetradecameric mitochondrial chaperonin; required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12493772]
EXISTENCE:direct assay:GO:0006665 sphingolipid metabolic process [PMID:9677363]
EXISTENCE:direct assay:GO:0009408 response to heat [PMID:11967828]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12493772]
EXISTENCE:direct assay:GO:0017050 D-erythro-sphingosine kinase activity [PMID:9677363]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:11278643]
Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
hypothetical protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect; decreased levels of protein in enolase deficient mutant
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:24194547]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:24924636]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:12401784]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:27462928]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:12401784|PMID:9880327]
EXISTENCE:mutant phenotype:GO:0000301 retrograde transport, vesicle recycling within Golgi [PMID:12401784]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:12401784]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:8923208]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:10990452|PMID:11160819|PMID:9880327]
Rab family GTPase; required for endosome-to-Golgi, intra-Golgi retrograde, and retrograde Golgi-to-ER transport; temporarily at the Golgi, dissociating into the cytosol on arrival of the late Golgi GTPase Ypt32p; Golgi-localized form is GTP bound, while cytosolic form is GDP-bound; required for delivery of Atg9p to the phagophore assembly site during autophagy under heat stress, with Ypt6p for starvation induced autophagy and for the CVT pathway; homolog of mammalian Rab6
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:16702403]
hypothetical protein that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000800 lateral element [PMID:9060462]
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:11779793]
EXISTENCE:direct assay:GO:0031490 chromatin DNA binding [PMID:12526806]
EXISTENCE:direct assay:GO:0043085 positive regulation of catalytic activity [PMID:14595109]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:9880561]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:10848609]
Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:3533916]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916]
EXISTENCE:mutant phenotype:GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:24492965]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11676923]
EXISTENCE:direct assay:GO:0035861 site of double-strand break [PMID:21149442]
EXISTENCE:direct assay:GO:0045027 DNA end binding [PMID:21149442]
EXISTENCE:direct assay:GO:1904877 positive regulation of DNA ligase activity [PMID:21149442]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:21149442|PMID:11676923|PMID:18245831|PMID:31390569]
EXISTENCE:mutant phenotype:GO:0035825 homologous recombination [PMID:31390569]
EXISTENCE:mutant phenotype:GO:0045002 double-strand break repair via single-strand annealing [PMID:19571008]
EXISTENCE:mutant phenotype:GO:0045027 DNA end binding [PMID:31390569]
EXISTENCE:physical interaction:GO:0032807 DNA ligase IV complex [PMID:17567543]
DNA end-binding protein required for nonhomologous end joining (NHEJ); subunit of the DNA ligase IV complex involved in initial end binding, intermolecular ligation, and DNA ligase reactivation after completion of repair; phosphorylation by Tpk1p is required for DNA damage induced NHEJ and for nuclear targeting of Nej1p and Lif1p; human homolog NHEJ1 is phosphorylated by human Tpk1p homolog PRKACB, regulating NHEJ; transcriptionally repressed by MAT heterozygosity
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12529331]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12529331]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12529331]
Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0048279 vesicle fusion with endoplasmic reticulum [PMID:9214619]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9580559|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:9580559]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1903839]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12221135]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9813082]
EXISTENCE:mutant phenotype:GO:0048280 vesicle fusion with Golgi apparatus [PMID:12221135]
EXISTENCE:physical interaction:GO:0005484 SNAP receptor activity [PMID:12853481]
R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA) diphosphatase
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:12198172]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:16963086]
EXISTENCE:direct assay:GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides [PMID:12198172]
EXISTENCE:direct assay:GO:0044692 exoribonuclease activator activity [PMID:22570495]
EXISTENCE:direct assay:GO:0050072 m7G(5')pppN diphosphatase activity [PMID:16963086]
EXISTENCE:direct assay:GO:1901919 positive regulation of exoribonuclease activity [PMID:22570495]
EXISTENCE:genetic interaction:GO:0009267 cellular response to starvation [PMID:15240832]
EXISTENCE:genetic interaction:GO:0031086 nuclear-transcribed mRNA catabolic process, deadenylation-independent decay [PMID:16260594]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:12198172]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:15240832]
EXISTENCE:mutant phenotype:GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides [PMID:12198172]
EXISTENCE:mutant phenotype:GO:0031086 nuclear-transcribed mRNA catabolic process, deadenylation-independent decay [PMID:16260594]
Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11854418]
EXISTENCE:genetic interaction:GO:0003682 chromatin binding [PMID:11864994]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:11854418|PMID:11864994]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:11854418|PMID:11864994]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0010032 meiotic chromosome condensation [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11854418]
EXISTENCE:mutant phenotype:GO:0043007 maintenance of rDNA [PMID:17203076]
EXISTENCE:mutant phenotype:GO:0044804 autophagy of nucleus [PMID:31553911]
EXISTENCE:mutant phenotype:GO:0051307 meiotic chromosome separation [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:31553911]
EXISTENCE:mutant phenotype:GO:1903342 negative regulation of meiotic DNA double-strand break formation [PMID:25103240]
Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:genetic interaction:GO:0005979 regulation of glycogen biosynthetic process [PMID:9046081]
EXISTENCE:genetic interaction:GO:0019888 protein phosphatase regulator activity [PMID:9046081]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:9046081]
Putative targeting subunit for type-1 protein phosphatase Glc7p; tethers Glc7p to Gsy2p glycogen synthase; PIG1 has a paralog, GAC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:19005221]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10075735|PMID:9159120]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2279699]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:2279699]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:19318354]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:18657510]
EXISTENCE:direct assay:GO:0042555 MCM complex [PMID:12480933]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:17895243]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:2279699]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:9990508]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:12750362]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16980387]
EXISTENCE:mutant phenotype:GO:0033260 nuclear DNA replication [PMID:2044962]
EXISTENCE:physical interaction:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:15028736]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:15028736]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11565753]
EXISTENCE:direct assay:GO:0033677 DNA/RNA helicase activity [PMID:15028736]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11565753]
DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
cleavage polyadenylation factor subunit YSH1
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:15037765]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:18971324]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:18971324]
EXISTENCE:mutant phenotype:GO:0008380 RNA splicing [PMID:18971324]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:18971324]
EXISTENCE:mutant phenotype:GO:0034247 snoRNA splicing [PMID:18971324]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:18971324]
Endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; associates with rDNA chromatin in vivo; YLR278C is not an essential gene
EXISTENCE:direct assay:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:33686140]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:17054397]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:33686140]
Stalled-ribosome rescue factor srRF2; mitochondrial protein required for respiratory growth in presence of bacteriostatic ribosome-binding antibiotics; not an essential gene
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:32978391]
EXISTENCE:mutant phenotype:GO:2000214 regulation of proline metabolic process [PMID:32978391]
Regulator of mitochondrial proline metabolism; tethered with Fmp32p to inner mitochondrial membrane in large hetero-oligomeric complex, abundance of which is regulated by proline; involved in mitochondrial proline homeostasis and cellular redox balance; null exhibits pronounced defect in proline utilization, and can be functionally complemented by expression of human homolog MCUR1
EXISTENCE:direct assay:GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity [PMID:9813046|PMID:9837886]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:9813046|PMID:9837886]
EXISTENCE:direct assay:GO:0006635 fatty acid beta-oxidation [PMID:9813046|PMID:9837886]
EXISTENCE:mutant phenotype:GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity [PMID:9813046|PMID:9837886]
Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; ECI1 has a paralog, DCI1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:21858014]
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:26545399]
EXISTENCE:direct assay:GO:0071885 N-terminal protein N-methyltransferase activity [PMID:26545399]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:12736687]
S-adenosylmethionine-dependent methyltransferase; novel N-terminal protein methyltransferase that trimethylates the N-terminal glycine residue (G2) and also dimethylates lysine (K3) on elongation factor eEF1A (Tef1p/Tef2p); has a role in rDNA silencing and in lifespan determination
EXISTENCE:curator inference:GO:0006032 chitin catabolic process [PMID:6799506]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:1918080|PMID:19129178]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11747810]
EXISTENCE:direct assay:GO:0008843 endochitinase activity [PMID:6799506]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11747810|PMID:1918080]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:1918080]
Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:19470581|PMID:26777405]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:10915872]
EXISTENCE:direct assay:GO:0030896 checkpoint clamp complex [PMID:12604797|PMID:9670034|PMID:9891048]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:17202155]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:9725831]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:9988274]
DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16415861]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:18442968]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:18442968]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:18442968]
Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:1901006 ubiquinone-6 biosynthetic process [PMID:25631044]
Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes; required for synthesis of wild-type levels of Coenzyme Q (ubiquinone); member of the short-chain dehydrogenase/reductase (SDR) superfamily; orthologous gene in some other fungi is fused to the COQ10 ortholog
translation initiation factor eIF2B subunit beta
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8506384]
EXISTENCE:direct assay:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705|PMID:8506384]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:8506384]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8506384]
EXISTENCE:genetic interaction:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:genetic interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705]
EXISTENCE:genetic interaction:GO:0006446 regulation of translational initiation [PMID:8164676|PMID:8887689]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:8164676|PMID:8887689]
EXISTENCE:mutant phenotype:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8441423]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:9032257]
EXISTENCE:mutant phenotype:GO:0030234 enzyme regulator activity [PMID:9032257]
EXISTENCE:physical interaction:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8336705]
EXISTENCE:physical interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705|PMID:8506384]
EXISTENCE:physical interaction:GO:0006446 regulation of translational initiation [PMID:8887689]
EXISTENCE:physical interaction:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8336705]
Beta subunit of translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as negative regulator of GCN4 expression; mutations in human ortholog EIF2B2 cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); human ortholog can complement yeast mutant, allows growth down-regulation of yeast gene
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:genetic interaction:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:32513868]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:32513868]
EXISTENCE:physical interaction:GO:0031207 Sec62/Sec63 complex [PMID:8051213]
Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and translocation into the ER; other members are Sec63p, Sec62p, and Sec66p
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:7744835|PMID:7816822|PMID:9305944]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8455603|PMID:11024003]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11024003]
EXISTENCE:direct assay:GO:0046822 regulation of nucleocytoplasmic transport [PMID:29146254]
EXISTENCE:direct assay:GO:1901987 regulation of cell cycle phase transition [PMID:29146254]
EXISTENCE:genetic interaction:GO:0006997 nucleus organization [PMID:8455603]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11404326]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:7816822]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:7816822]
Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication
ARS1221; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18722382]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:8702761]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:8702761]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:18252710]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:18252710]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:8702761]
Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0030627 pre-mRNA 5'-splice site binding [PMID:12214237]
EXISTENCE:direct assay:GO:1905216 positive regulation of RNA binding [PMID:28829039]
EXISTENCE:mutant phenotype:GO:0000395 mRNA 5'-splice site recognition [PMID:12214237]
Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:6143574]
EXISTENCE:mutant phenotype:GO:0006751 glutathione catabolic process [PMID:11672438]
EXISTENCE:mutant phenotype:GO:0006805 xenobiotic metabolic process [PMID:16857395]
EXISTENCE:mutant phenotype:GO:0036374 glutathione hydrolase activity [PMID:11672438]
Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:19129178]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9748433]
EXISTENCE:mutant phenotype:GO:0006073 cellular glucan metabolic process [PMID:7565587]
Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication
EXISTENCE:physical interaction:GO:0005789 endoplasmic reticulum membrane [PMID:12518317]
EXISTENCE:physical interaction:GO:0006612 protein targeting to membrane [PMID:12518317]
hypothetical protein that interacts with Sec72p and Hrr25p
hypothetical protein; conserved among S. cerevisiae strains
bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase MET17
EXISTENCE:direct assay:GO:0003961 O-acetylhomoserine aminocarboxypropyltransferase activity [PMID:7765825]
EXISTENCE:direct assay:GO:0004124 cysteine synthase activity [PMID:7765825]
EXISTENCE:mutant phenotype:GO:0006555 methionine metabolic process [PMID:1101032]
O-acetyl homoserine-O-acetyl serine sulfhydrylase; involved in methionine and cysteine biosynthesis; long considered essential for inorganic sulfur assimilation, but inefficient homocysteine synthase Hsu1p enables met17 null cells to assimilate enough inorganic sulfur for survival and proliferation; growth failure of met17 null can be explained by toxic accumulation of H2S gas due to a metabolic bottleneck
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:17698960]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:17698960]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:18959479]
EXISTENCE:direct assay:GO:0003994 aconitate hydratase activity [PMID:1972545]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:15692048]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15975908]
EXISTENCE:direct assay:GO:0006099 tricarboxylic acid cycle [PMID:1972545]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0003994 aconitate hydratase activity [PMID:1972545]
Aconitase; required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy; mutation in human homolog ACO2 is associated with dominant optic nerve atrophy; human homolog ACO2 can complement yeast null mutant
EXISTENCE:direct assay:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:9346907]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12015967|PMID:19075114]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:9346907]
EXISTENCE:genetic interaction:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:10930462]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:22977244]
EXISTENCE:mutant phenotype:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:8288577]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:22977244]
EXISTENCE:mutant phenotype:GO:0061909 autophagosome-lysosome fusion [PMID:31125705]
EXISTENCE:mutant phenotype:GO:0140504 microlipophagy [PMID:32726584]
Phosphatidylinositol-4-kinase; functions in Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization; required for autophagosome-vacuole fusion during autophagy and for lipophagy in stationary phase cells and during nitrogen starvation; localizes to plasma membrane and mitochondria in HTP studies
EXISTENCE:direct assay:GO:0019788 NEDD8 transferase activity [PMID:9545234]
EXISTENCE:direct assay:GO:0045116 protein neddylation [PMID:9545234]
EXISTENCE:mutant phenotype:GO:0019788 NEDD8 transferase activity [PMID:9545234]
EXISTENCE:mutant phenotype:GO:0045116 protein neddylation [PMID:9545234]
Enzyme that mediates the conjugation of Rub1p; a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
ARS1223; Autonomously Replicating Sequence; replication capacity dependent upon Sum1p
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:genetic interaction:GO:0004099 chitin deacetylase activity [PMID:8940152]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:10544249|PMID:8940152]
EXISTENCE:mutant phenotype:GO:0004099 chitin deacetylase activity [PMID:8940152|PMID:9133736]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:10544249]
Chitin deacetylase; together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
Putative mitochondrial hypothetical protein
Chitin deacetylase; together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15077113]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9880327]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:9880327]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:16926193]
Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
Ras family guanine nucleotide exchange factor CDC25
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8206969|PMID:9047390|PMID:1569942|PMID:21073870]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18930081]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1334534]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:22575457]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18930081]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8893498]
EXISTENCE:direct assay:GO:0007265 Ras protein signal transduction [PMID:9047390]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8206969|PMID:9047390]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:3021894]
EXISTENCE:mutant phenotype:GO:0007265 Ras protein signal transduction [PMID:1849965|PMID:9047390]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:3021894]
Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain
EXISTENCE:direct assay:GO:0005640 nuclear outer membrane [PMID:35061008]
EXISTENCE:direct assay:GO:0097038 perinuclear endoplasmic reticulum [PMID:26040717]
EXISTENCE:genetic interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:26040717]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:26040717]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:26040717]
EXISTENCE:mutant phenotype:GO:0044804 autophagy of nucleus [PMID:26040717]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26040717]
Transmembrane receptor with a role in nucleophagy; involved in selective autophagy of the endoplasmic reticulum and nucleus in response to nitrogen starvation or rapamycin treatment; role in nuclear envelope deformation to generate nucleus-derived double-membrane vesicles (NDVs), interacting with Atg8p to target these NDVs to forming autophagosomal membranes; anchored in the outer nuclear membrane, associating with the inner nuclear membrane via amphipathic helices
mitochondrial 54S ribosomal protein YmL15
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:9151978|PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9443897|PMID:9214378]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9443897|PMID:15282802|PMID:9214378]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9443897|PMID:15282802|PMID:9214378]
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:11923536|PMID:9214378]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:9214378]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9443897]
Protein involved in shmoo formation and bipolar bud site selection; localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; SPH1 has a paralog, SPA2, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0030011 maintenance of cell polarity [PMID:10882120]
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:1993729]
EXISTENCE:direct assay:GO:0000281 mitotic cytokinesis [PMID:12636916]
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:8791410]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:18550837|PMID:22144691|PMID:29321251]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14597621]
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:8636217]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18826657]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18550837]
EXISTENCE:direct assay:GO:0032160 septin filament array [PMID:15282341]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:12665577]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:4950437]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:15282341]
EXISTENCE:mutant phenotype:GO:0031107 septin ring disassembly [PMID:12429908]
EXISTENCE:physical interaction:GO:0031105 septin complex [PMID:9813094]
Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:12408861]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:24385296]
Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:10550050]
EXISTENCE:direct assay:GO:0008251 tRNA-specific adenosine deaminase activity [PMID:10550050]
Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs; localizes to cytosol, nucleus and peroxisomes
EXISTENCE:direct assay:GO:0000333 telomerase catalytic core complex [PMID:15813705]
EXISTENCE:direct assay:GO:0003720 telomerase activity [PMID:15813705]
EXISTENCE:direct assay:GO:0007004 telomere maintenance via telomerase [PMID:10898986|PMID:15813705]
EXISTENCE:mutant phenotype:GO:0003721 telomerase RNA reverse transcriptase activity [PMID:9110970]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:15813705|PMID:8978029]
Reverse transcriptase subunit of the telomerase holoenzyme; essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11071900|PMID:9247651]
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:7719850|PMID:9247651]
EXISTENCE:direct assay:GO:0005519 cytoskeletal regulatory protein binding [PMID:9082982]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:17416900]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11071900|PMID:23671312|PMID:9247651]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11071900|PMID:23671312|PMID:9247651]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9082982|PMID:19053807]
EXISTENCE:direct assay:GO:0051127 positive regulation of actin nucleation [PMID:30076201]
EXISTENCE:direct assay:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:15923184|PMID:14657240]
EXISTENCE:direct assay:GO:0099503 secretory vesicle [PMID:30076201]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:genetic interaction:GO:0051127 positive regulation of actin nucleation [PMID:30076201]
EXISTENCE:genetic interaction:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:14657240]
EXISTENCE:genetic interaction:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:8657162|PMID:9247651]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:23671312]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:9247651]
EXISTENCE:mutant phenotype:GO:0030953 astral microtubule organization [PMID:22608510]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:10085294]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521]
EXISTENCE:physical interaction:GO:0005519 cytoskeletal regulatory protein binding [PMID:15923184]
Actin- and formin-interacting protein; nucleation-promoting factor (NPF) for Bni1p and Bnr1p during actin cable assembly and organization; enhances Bnr1p-mediated actin nucleation, alleviating Hof1p inhibition through competitive binding; interacts with Bni1p via a C-terminal triple helical coiled-coil domain; role in polarized cell growth; isolated as a bipolar budding mutant; localizes to the presumptive bud site, small bud tips, the bud neck and secretory vesicles; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15718301]
EXISTENCE:direct assay:GO:0035361 Cul8-RING ubiquitin ligase complex [PMID:20139071]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:18321796]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:20174551]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:20174551]
EXISTENCE:mutant phenotype:GO:0031297 replication fork processing [PMID:18704118]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:18069899]
Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of replication fork, such as fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; Rtt101p-Mms22p ligase associates with replisome complex during S phase via Ctf4p; required for accurate meiotic chromosome segregation
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:31672915]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366|PMID:31672915]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:31672915]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:12697820]
EXISTENCE:genetic interaction:GO:0033262 regulation of nuclear cell cycle DNA replication [PMID:17542652]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16024655]
EXISTENCE:mutant phenotype:GO:0031055 chromatin remodeling at centromere [PMID:12697820]
Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog; hSNF5 tumor suppressor ortholog
U2-type spliceosomal complex subunit CWC24
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17974558]
EXISTENCE:direct assay:GO:0005684 U2-type spliceosomal complex [PMID:11884590]
EXISTENCE:mutant phenotype:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:22535589]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:23029180]
EXISTENCE:mutant phenotype:GO:0034247 snoRNA splicing [PMID:23029180|PMID:17974558]
EXISTENCE:physical interaction:GO:0005681 spliceosomal complex [PMID:23029180]
General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27129769|PMID:14562095]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:14617799]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:27129769|PMID:26928762]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0071782 endoplasmic reticulum tubular network [PMID:27872254]
EXISTENCE:genetic interaction:GO:0032581 ER-dependent peroxisome organization [PMID:27129769]
EXISTENCE:genetic interaction:GO:1900063 regulation of peroxisome organization [PMID:27872254]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:14617799]
EXISTENCE:mutant phenotype:GO:0097749 membrane tubulation [PMID:27872254]
ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex29p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; partially redundant with Pex31p; may function at a step downstream of steps mediated by Pex28p and Pex29p; PEX30 has a paralog, PEX31, that arose from the whole genome duplication
Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; predicted to be palmitoylated
hypothetical protein that associates with ribosomes; protein abundance increases in response to DNA replication stress; TMA10 has a paralog, STF2, that arose from the whole genome duplication
tRNA of undetermined specificity, predicted by tRNAscan-SE analysis; very similar to serine tRNAs
nicotinamide-nucleotide adenylyltransferase NMA1
EXISTENCE:direct assay:GO:0000309 nicotinamide-nucleotide adenylyltransferase activity [PMID:10428462]
EXISTENCE:direct assay:GO:0009435 NAD biosynthetic process [PMID:10428462]
Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:15044957]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:1732169]
Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9008706|PMID:24390141]
EXISTENCE:direct assay:GO:0006355 regulation of DNA-templated transcription [PMID:17000877]
EXISTENCE:direct assay:GO:0034044 exomer complex [PMID:23000721|PMID:17000877]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:16498409]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:9111317]
EXISTENCE:mutant phenotype:GO:0006039 cell wall chitin catabolic process [PMID:9111317]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:16498409|PMID:11879634]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:9111317]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:16498409]
Component of the exomer complex; the exomer which also contains Csh6p, Bch1p, Bch2p, and Bud7, is involved in the export of select proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus
hypothetical protein; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10348843]
EXISTENCE:genetic interaction:GO:0006970 response to osmotic stress [PMID:14871941]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:10348843]
EXISTENCE:mutant phenotype:GO:0004888 transmembrane signaling receptor activity [PMID:10330137]
EXISTENCE:mutant phenotype:GO:0010447 response to acidic pH [PMID:16087742]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:11014808]
EXISTENCE:mutant phenotype:GO:0036498 IRE1-mediated unfolded protein response [PMID:18971375]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:24460703]
O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication
ARS1226; Autonomously Replicating Sequence
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A1133
C/D box small nucleolar RNA (snoRNA); predicted to guide 2'-O-methylation of small subunit (SSU) rRNA at position U1269
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position G1572
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0006607 NLS-bearing protein import into nucleus [PMID:11046143|PMID:11867631]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:11425876]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:10725229|PMID:11425876]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0006611 protein export from nucleus [PMID:10542277]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:11046143]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:10542277|PMID:10725229|PMID:11046143]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:21036941]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21818277]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16365162]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809]
EXISTENCE:mutant phenotype:GO:0036228 protein localization to nuclear inner membrane [PMID:16929305]
EXISTENCE:physical interaction:GO:0006611 protein export from nucleus [PMID:10725229]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:11046143|PMID:10525531|PMID:12917401|PMID:17418788]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:7638224]
Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11042259]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11042259]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:11042259|PMID:12073033]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:19806183]
EXISTENCE:physical interaction:GO:0097078 FAL1-SGD1 complex [PMID:21576267]
Essential nuclear protein; required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9412475]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9412475]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:9412475|PMID:16824951]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:11604421|PMID:16824951]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:9742397|PMID:8590801]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:9412475]
EXISTENCE:mutant phenotype:GO:0032465 regulation of cytokinesis [PMID:15941409]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:8590801]
EXISTENCE:physical interaction:GO:0003779 actin binding [PMID:9412475]
Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19797078]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:16164560]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:8195220]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:8195220]
Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15590821]
EXISTENCE:mutant phenotype:GO:1903024 positive regulation of ascospore-type prospore membrane formation [PMID:27182947]
Meiosis-specific hypothetical protein; required for spore wall formation during sporulation and for timely prospore membrane closure along with SPS1; required with Sps1p for phosphorylation and turnover of Ssp1p; dispensable for both nuclear divisions during meiosis
EXISTENCE:direct assay:GO:0000148 1,3-beta-D-glucan synthase complex [PMID:7983071]
EXISTENCE:direct assay:GO:0003843 1,3-beta-D-glucan synthase activity [PMID:7528927|PMID:7649185]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11856368|PMID:17507646]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11856368|PMID:8602515]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11856368|PMID:8602515]
EXISTENCE:direct assay:GO:0006075 (1->3)-beta-D-glucan biosynthetic process [PMID:7528927]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:11856368]
EXISTENCE:mutant phenotype:GO:0003843 1,3-beta-D-glucan synthase activity [PMID:7528927]
EXISTENCE:mutant phenotype:GO:0006075 (1->3)-beta-D-glucan biosynthetic process [PMID:7528927]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:17302939]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:14593073]
Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0042124 1,3-beta-glucanosyltransferase activity [PMID:17397106]
EXISTENCE:direct assay:GO:0070880 fungal-type cell wall beta-glucan biosynthetic process [PMID:19097997]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:17189486]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:17189486]
1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; single essential nuclear tRNA gene containing the tDNA-anticodon CCG (presumably CCG in the mature tRNA), decodes CGG codons into arginine, one of 19 nuclear tRNAs for arginine; correct folding and efficient aminoacylation require Lhp1p
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943|PMID:9672752]
Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
bifunctional fructose-2,6-bisphosphate 2-phosphatase/6-phosphofructo-2-kinase
Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
hypothetical protein found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:19549784]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:direct assay:GO:0042564 NLS-dependent protein nuclear import complex [PMID:19141610]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:19046973]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:7622450]
EXISTENCE:genetic interaction:GO:0006656 phosphatidylcholine biosynthetic process [PMID:19141610]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:23131016|PMID:19141610]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:16929305]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:19141610]
EXISTENCE:mutant phenotype:GO:0051292 nuclear pore complex assembly [PMID:17182855]
EXISTENCE:mutant phenotype:GO:0060188 regulation of protein desumoylation [PMID:17403926]
Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
EXISTENCE:direct assay:GO:0005310 dicarboxylic acid transmembrane transporter activity [PMID:9020177|PMID:9559855]
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:15591051]
EXISTENCE:direct assay:GO:0006835 dicarboxylic acid transport [PMID:9559855|PMID:9020177]
Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20182505|PMID:14562095]
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:genetic interaction:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:20182505]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:12093374]
EXISTENCE:mutant phenotype:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:20182505]
Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides [PMID:28373563]
EXISTENCE:direct assay:GO:0043605 amide catabolic process [PMID:28373563]
Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:11283612]
hypothetical protein with similarity to F-box proteins; interacts with the core SCF ubiquitin ligase complex subunits Skp1p and Cdc53p; LUG1 deletion prevents cells containing the [URE3] prion or lacking Ure2p from growing on glycerol
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11514631]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11514631]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:8657162]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:17581861]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication
sedoheptulose-7-phosphate:D-glyceraldehyde-3- phosphate transaldolase TAL1
EXISTENCE:direct assay:GO:0004801 transaldolase activity [PMID:2185015]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17397208]
EXISTENCE:mutant phenotype:GO:0006098 pentose-phosphate shunt [PMID:8299150]
Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:18023287]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:18193418|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:18826960]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:7621838]
EXISTENCE:mutant phenotype:GO:0004455 ketol-acid reductoisomerase activity [PMID:14124940|PMID:3027658]
EXISTENCE:mutant phenotype:GO:0009082 branched-chain amino acid biosynthetic process [PMID:14124940]
Acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19793921|PMID:14562095|PMID:24769239|PMID:26928762]
Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23907388]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:14729968]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:16024655]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:14729968]
EXISTENCE:mutant phenotype:GO:0070914 UV-damage excision repair [PMID:24360959]
EXISTENCE:physical interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication
hypothetical protein; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
EXISTENCE:genetic interaction:GO:0006189 'de novo' IMP biosynthetic process [PMID:7896125]
EXISTENCE:mutant phenotype:GO:0004018 N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity [PMID:5807803|PMID:7896125]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:5807803]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:5807803]
Adenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency; human ADSL can complement yeast ADE13 null mutant
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:9265642|PMID:11157979]
Part of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:16990850|PMID:17673172]
EXISTENCE:genetic interaction:GO:1900102 negative regulation of endoplasmic reticulum unfolded protein response [PMID:16990850]
EXISTENCE:mutant phenotype:GO:0004721 phosphoprotein phosphatase activity [PMID:17673172]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:15590836]
EXISTENCE:mutant phenotype:GO:0016788 hydrolase activity, acting on ester bonds [PMID:15590836]
EXISTENCE:mutant phenotype:GO:1900102 negative regulation of endoplasmic reticulum unfolded protein response [PMID:16990850]
Protein phosphatase; involved in downregulation of the unfolded protein response (UPR), at least in part through dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START; physically interacts with, dephosphorylates and destabilizes Sic1p; SWAT-GFP and mCherry fusion proteins localize to the vacuole
mitogen-activated protein kinase kinase kinase STE11
EXISTENCE:direct assay:GO:0004709 MAP kinase kinase kinase activity [PMID:8159759]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9755161]
EXISTENCE:direct assay:GO:0032093 SAM domain binding [PMID:16337230]
EXISTENCE:direct assay:GO:0071507 pheromone response MAPK cascade [PMID:8159759]
EXISTENCE:genetic interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:9180081]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:10880465]
EXISTENCE:genetic interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:9180081]
EXISTENCE:genetic interaction:GO:0071507 pheromone response MAPK cascade [PMID:8455599]
EXISTENCE:mutant phenotype:GO:0000161 osmosensory signaling MAPK cascade [PMID:9180081]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8455599]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:8259520]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:8001818]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8259520]
EXISTENCE:mutant phenotype:GO:0010525 regulation of transposition, RNA-mediated [PMID:9566871]
EXISTENCE:mutant phenotype:GO:0071474 cellular hyperosmotic response [PMID:9180081]
EXISTENCE:mutant phenotype:GO:0071507 pheromone response MAPK cascade [PMID:8159759]
Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress
EXISTENCE:physical interaction:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:7883168]
Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
EXISTENCE:direct assay:GO:0004362 glutathione-disulfide reductase (NADPH) activity [PMID:19166312]
Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
hypothetical protein; conserved among S. cerevisiae strains; YLR365W is not an essential gene
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1056 and small subunit (SSU) rRNA at position U106
SCF ubiquitin ligase complex subunit MDM30
EXISTENCE:direct assay:GO:0051603 proteolysis involved in protein catabolic process [PMID:16735578]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:18353967]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:18353967]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266]
EXISTENCE:mutant phenotype:GO:0008053 mitochondrial fusion [PMID:16790496|PMID:12808031]
EXISTENCE:mutant phenotype:GO:0010793 regulation of mRNA export from nucleus [PMID:19376128]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:18353967]
EXISTENCE:mutant phenotype:GO:0051603 proteolysis involved in protein catabolic process [PMID:16735578]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994|PMID:18353967]
EXISTENCE:physical interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:18353967]
F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8707841]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12756240]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:11601843]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9660806]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:9813017]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:11171977]
EXISTENCE:mutant phenotype:GO:0106034 protein maturation by [2Fe-2S] cluster transfer [PMID:11273703]
Mitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis; involved in maturation of Grx5p and Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16107558]
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0051654 establishment of mitochondrion localization [PMID:16107558]
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
Rho family guanine nucleotide exchange factor ROM2
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9348527]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8641285]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:12015967]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9348527]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9348527]
EXISTENCE:direct assay:GO:0090630 activation of GTPase activity [PMID:8641285]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:9545237]
EXISTENCE:genetic interaction:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:16763112]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:9348527]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8641285]
EXISTENCE:physical interaction:GO:0007264 small GTPase mediated signal transduction [PMID:8641285]
Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12087109]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:23325927]
EXISTENCE:mutant phenotype:GO:0006633 fatty acid biosynthetic process [PMID:9211877]
EXISTENCE:mutant phenotype:GO:0006892 post-Golgi vesicle-mediated transport [PMID:9832547]
EXISTENCE:mutant phenotype:GO:0009922 fatty acid elongase activity [PMID:12684876]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9211877]
EXISTENCE:mutant phenotype:GO:0030497 fatty acid elongation [PMID:9211877]
EXISTENCE:mutant phenotype:GO:0042761 very long-chain fatty acid biosynthetic process [PMID:23325927]
Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141|PMID:26443863]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11861771]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:34871443]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:23222485]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23222485]
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:11861771]
G-quadruplex (G4) DNA binding protein; maintains genome integrity by promoting the stability of G4-DNA regions; involved in nucleosome displacement during double-strand break repair; involved in fructose-1,6-bisphosphatase (FBPase) transport from the cytosol to Vid (vacuole import and degradation) vesicles during degradation; glycosylated integral membrane protein of the plasma membrane; localizes to the nucleus; contains a BED-type zinc finger domain and a RNase H-like domain
Zinc-finger hypothetical protein; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15654096|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0097196 Shu complex [PMID:23575680]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:15654096]
EXISTENCE:genetic interaction:GO:0070987 error-free translesion synthesis [PMID:19496932]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0000730 DNA recombinase assembly [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0035861 site of double-strand break [PMID:23575680]
EXISTENCE:physical interaction:GO:0097196 Shu complex [PMID:15654096]
Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12686616|PMID:196563]
EXISTENCE:direct assay:GO:0042132 fructose 1,6-bisphosphate 1-phosphatase activity [PMID:6304022]
EXISTENCE:direct assay:GO:0042597 periplasmic space [PMID:22833678]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:6321183]
EXISTENCE:mutant phenotype:GO:0042132 fructose 1,6-bisphosphate 1-phosphatase activity [PMID:2841162]
EXISTENCE:mutant phenotype:GO:0072593 reactive oxygen species metabolic process [PMID:16199065]
Fructose-1,6-bisphosphatase; key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; glucose starvation results in redistribution to the periplasm; interacts with Vid30p
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005048 signal sequence binding [PMID:12134063]
EXISTENCE:direct assay:GO:0005784 Sec61 translocon complex [PMID:8612571|PMID:9252322]
EXISTENCE:direct assay:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:11076036]
EXISTENCE:direct assay:GO:0015450 protein-transporting ATPase activity [PMID:9252322]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110|PMID:9252322]
EXISTENCE:mutant phenotype:GO:0015450 protein-transporting ATPase activity [PMID:1550957]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:24314051]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:10635318|PMID:9303298]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:9843581|PMID:32513868]
EXISTENCE:mutant phenotype:GO:0044743 protein transmembrane import into intracellular organelle [PMID:24314051]
EXISTENCE:mutant phenotype:GO:0070843 misfolded protein transport [PMID:24314051]
EXISTENCE:mutant phenotype:GO:1904680 peptide transmembrane transporter activity [PMID:10758167]
EXISTENCE:physical interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:19696741]
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex is required for SRP-independent protein translocation into the ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
Novel ORF identified via pan-genome analysis; conserved in other strains of S. cerevisiae
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12869188|PMID:10848624]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:17803462]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12869188|PMID:26928762]
EXISTENCE:direct assay:GO:0008526 phosphatidylinositol transfer activity [PMID:10848624|PMID:31690622]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:10848624]
EXISTENCE:direct assay:GO:0045717 negative regulation of fatty acid biosynthetic process [PMID:17803462]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16126894]
EXISTENCE:genetic interaction:GO:0043001 Golgi to plasma membrane protein transport [PMID:10848624]
EXISTENCE:genetic interaction:GO:0045717 negative regulation of fatty acid biosynthetic process [PMID:17803462]
EXISTENCE:genetic interaction:GO:0046488 phosphatidylinositol metabolic process [PMID:16262726]
EXISTENCE:genetic interaction:GO:1901352 negative regulation of phosphatidylglycerol biosynthetic process [PMID:12869188]
EXISTENCE:genetic interaction:GO:2001247 positive regulation of phosphatidylcholine biosynthetic process [PMID:12869188]
EXISTENCE:mutant phenotype:GO:0045717 negative regulation of fatty acid biosynthetic process [PMID:17803462]
Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11782448]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:20980819]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11782448]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:22426531]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6
EXISTENCE:direct assay:GO:0004823 leucine-tRNA ligase activity [PMID:1990003]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1990003|PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0006429 leucyl-tRNA aminoacylation [PMID:1990003]
EXISTENCE:genetic interaction:GO:0032543 mitochondrial translation [PMID:16777356]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:2277640|PMID:12486008]
EXISTENCE:physical interaction:GO:0097157 pre-mRNA intronic binding [PMID:11142386]
Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:16793545]
EXISTENCE:direct assay:GO:0035861 site of double-strand break [PMID:16892052]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:16892052]
EXISTENCE:mutant phenotype:GO:0051304 chromosome separation [PMID:15793567]
EXISTENCE:mutant phenotype:GO:0071139 resolution of recombination intermediates [PMID:20159973]
EXISTENCE:mutant phenotype:GO:1990683 DNA double-strand break attachment to nuclear envelope [PMID:27056668]
Subunit of the SMC5-SMC6 complex; the SMC5-SMC6 complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; homologous to S. pombe rad18
ARS1238; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:24750273]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12139626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12139626]
EXISTENCE:direct assay:GO:0033588 elongator holoenzyme complex [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11689709]
Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14690608]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:14690608]
hypothetical protein; component of the Swr1p complex that incorporates Htz1p into chromatin
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14528018|PMID:11889142|PMID:23708375]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:19037259]
EXISTENCE:direct assay:GO:0070772 PAS complex [PMID:19037259]
EXISTENCE:genetic interaction:GO:0006661 phosphatidylinositol biosynthetic process [PMID:11889142]
EXISTENCE:genetic interaction:GO:0090218 positive regulation of lipid kinase activity [PMID:11889142|PMID:14528018]
EXISTENCE:mutant phenotype:GO:0006661 phosphatidylinositol biosynthetic process [PMID:11889142]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:19037259]
EXISTENCE:mutant phenotype:GO:0090218 positive regulation of lipid kinase activity [PMID:11889142]
EXISTENCE:mutant phenotype:GO:1903778 protein localization to vacuolar membrane [PMID:14528018]
EXISTENCE:physical interaction:GO:0070772 PAS complex [PMID:19037259]
Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
EXISTENCE:genetic interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:19433447]
Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:18782943|PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480|PMID:18782943]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480|PMID:18782943]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0000454 snoRNA guided rRNA pseudouridine synthesis [PMID:15923376|PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748|PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2826 and U2880
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:19750477]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:28228553]
EXISTENCE:genetic interaction:GO:0034982 mitochondrial protein processing [PMID:28228553]
EXISTENCE:genetic interaction:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:7569998]
Metalloprotease; involved in N-terminal processing of pro-a-factor to mature form; expressed in both haploids and diploids; one of two yeast homologs of human insulin-degrading enzyme (hIDE); homolog Axl1p is also involved in processing of pro-a-factor
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:9079899]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460|PMID:9079899|PMID:9301021]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9079899]
Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall
hypothetical protein that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:22842922|PMID:24769239]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:2141026]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:14998992]
EXISTENCE:physical interaction:GO:0005743 mitochondrial inner membrane [PMID:14998992]
EXISTENCE:physical interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:14998992]
EXISTENCE:physical interaction:GO:0051082 unfolded protein binding [PMID:14998992]
Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:10943844]
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:19056898]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16782016]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23593021]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:16847351]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:16847351]
EXISTENCE:mutant phenotype:GO:0003682 chromatin binding [PMID:23593021]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:15805472|PMID:10943844]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:16847351|PMID:10943844]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15066280|PMID:23593021]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:16847351]
EXISTENCE:mutant phenotype:GO:0019789 SUMO transferase activity [PMID:16847351]
SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; associates with centromeres early in meiosis, then with chromosome axes and finally with double-strand break sites that are engaged in repair by crossovers; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
Subunit VIII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
CLS14; MET27; PEP14; SLP1; VAM5; VPL25; VPT33
tethering complex ATP-binding subunit VPS33
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11853670]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:9624182]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:9624182]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9624182]
EXISTENCE:genetic interaction:GO:0048278 vesicle docking [PMID:9245783]
EXISTENCE:genetic interaction:GO:0051469 vesicle fusion with vacuole [PMID:9245783]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:9624182]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:3062374]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:15047864|PMID:2183024]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:15047864]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:2183024]
EXISTENCE:physical interaction:GO:0000329 fungal-type vacuole membrane [PMID:10944212]
ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12006565]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17646390]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:9341149]
EXISTENCE:mutant phenotype:GO:0030687 preribosome, large subunit precursor [PMID:17646390]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:17646390]
ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0055087 Ski complex [PMID:10744028|PMID:18042677]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12769863|PMID:12881429]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:10611222|PMID:9482746]
EXISTENCE:mutant phenotype:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:11910110|PMID:17660569]
EXISTENCE:physical interaction:GO:0055087 Ski complex [PMID:18042677]
Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:7791775|PMID:34056863]
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:19088068]
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:12620225|PMID:17526728]
EXISTENCE:direct assay:GO:0001094 TFIID-class transcription factor complex binding [PMID:10783167]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12620225]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:12620224|PMID:20126658|PMID:34056863]
EXISTENCE:genetic interaction:GO:0031452 negative regulation of heterochromatin formation [PMID:16239142]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:7791775]
EXISTENCE:mutant phenotype:GO:0009301 snRNA transcription [PMID:7816623]
EXISTENCE:mutant phenotype:GO:0031452 negative regulation of heterochromatin formation [PMID:16239142]
EXISTENCE:mutant phenotype:GO:0070577 lysine-acetylated histone binding [PMID:34056863]
EXISTENCE:mutant phenotype:GO:0090054 regulation of silent mating-type cassette heterochromatin formation [PMID:12620225]
EXISTENCE:mutant phenotype:GO:1905168 positive regulation of double-strand break repair via homologous recombination [PMID:34056863]
EXISTENCE:physical interaction:GO:0001094 TFIID-class transcription factor complex binding [PMID:10783167]
BET family transcriptional regulator; associates with TFIID, and is involved in transcription initiation at TATA-containing promoters; essential for meiosis and expression of meiosis-specific genes; binds acetylated histone H4 at double-strand break (DSB) sites, and promotes end resection, loading of RPA and Rad51p and ds break repair by homologous recombination; contains two bromodomains that correspond to the C-terminal region of mammalian TAF1; partially redundant with paralog Bdf2p
ARS1228; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:14970222]
EXISTENCE:mutant phenotype:GO:0017150 tRNA dihydrouridine synthase activity [PMID:14970222]
Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
zinc-coordinating transcription factor SFP1
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:15353587]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15353587|PMID:15466158]
EXISTENCE:genetic interaction:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15466158]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:15353587]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:15466158|PMID:15806610]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12089449|PMID:9832520]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:14555489|PMID:15353587|PMID:15466158]
Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:18093937|PMID:31594806]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:24434579]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:18250201]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:32349126]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:18093937|PMID:18250201|PMID:25540432|PMID:26572621]
EXISTENCE:mutant phenotype:GO:0046889 positive regulation of lipid biosynthetic process [PMID:32349126]
EXISTENCE:mutant phenotype:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:31594806]
EXISTENCE:mutant phenotype:GO:0140042 lipid droplet formation [PMID:32349126]
Seipin involved in lipid droplet (LD) assembly; controls lipid particle morphology, number, and size; promotes initiation of LD formation on the ER; ensures that LDs bud from the ER towards the cytosolic side of the membrane; forms a complex with Ldb16p at ER-LD contact sites, stabilizing these sites; null mutants have localized accumulation of phosphatidic acid (PA) marker proteins; BSCL2, human homolog implicated in congenital lipodystrophy, complements yeast null mutant
Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
ARS1230; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:18829863]
Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
hypothetical protein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
hypothetical protein; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
EXISTENCE:curator inference:GO:0042274 ribosomal small subunit biogenesis [PMID:15590835]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835|PMID:22842922]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:19150991]
Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome
EXISTENCE:direct assay:GO:0000829 inositol heptakisphosphate kinase activity [PMID:17412958]
EXISTENCE:direct assay:GO:0000830 inositol hexakisphosphate 4-kinase activity [PMID:17412958]
EXISTENCE:direct assay:GO:0000831 inositol hexakisphosphate 6-kinase activity [PMID:17412958]
EXISTENCE:direct assay:GO:0032958 inositol phosphate biosynthetic process [PMID:17412958]
EXISTENCE:direct assay:GO:0052723 inositol hexakisphosphate 1-kinase activity [PMID:18981179]
EXISTENCE:direct assay:GO:0052724 inositol hexakisphosphate 3-kinase activity [PMID:18981179]
EXISTENCE:direct assay:GO:0052843 inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity [PMID:31436531]
EXISTENCE:direct assay:GO:0052845 inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity [PMID:31436531]
Bifunctional inositol pyrophosphate kinase and phosphatase; contains an N-terminal PP-InsP kinase domain that phosphorylates inositol hexakisphosphate and heptakisphosphate, and a C-terminal phosphatase domain that hydrolyzes both 1PP-InsP5 and 5PP-InsP5; IP7 levels decrease during starvation, suggesting a role for PP-InsP enzymes in Pi homeostasis; may regulate the dimorphic switch and the function of the cortical actin cytoskeleton
YLRWTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005375 copper ion transmembrane transporter activity [PMID:10924521]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10924521]
EXISTENCE:direct assay:GO:0015677 copper ion import [PMID:10924521]
High-affinity copper transporter of the plasma membrane; acts as a trimer; gene is disrupted by a Ty2 transposon insertion in many laboratory strains of S. cerevisiae
EXISTENCE:mutant phenotype:GO:0007017 microtubule-based process [PMID:18064466]
Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:24379413]
hypothetical protein; not an essential gene; YLR413W has a paralog, FAT3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18799621]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:21223946]
Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
hypothetical protein; YLR415C is not an essential gene
EXISTENCE:direct assay:GO:0000814 ESCRT II complex [PMID:12194858]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:19380877|PMID:15029239]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:11278625]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12194858]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:17428789|PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
Component of ESCRT-II complex; contains GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into endosome; plays role in lager yeast flocculation under brewing conditions; plays role in formation of mutant huntingtin (Htt) aggregates in yeast
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:11983171]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11983171]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15643076]
EXISTENCE:direct assay:GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding [PMID:22796944]
EXISTENCE:direct assay:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:0006362 transcription elongation by RNA polymerase I [PMID:19164765]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15149594]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:14710186]
EXISTENCE:mutant phenotype:GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [PMID:18469135]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:9891041]
EXISTENCE:mutant phenotype:GO:2001165 positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:18469135]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:11884586|PMID:9032243]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:11884586|PMID:11927560|PMID:9032243]
Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homolog, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
EXISTENCE:mutant phenotype:GO:0004151 dihydroorotase activity [PMID:2897615|PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006221 pyrimidine nucleotide biosynthetic process [PMID:2897615]
Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
proteasome regulatory particle lid subunit RPN13
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:11029046]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:18497817]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11029046|PMID:17499717]
Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors
EXISTENCE:direct assay:GO:0000045 autophagosome assembly [PMID:18725539]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:26774783]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:15901835]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18725539]
EXISTENCE:direct assay:GO:0034497 protein localization to phagophore assembly site [PMID:26774783]
EXISTENCE:direct assay:GO:0120095 vacuole-isolation membrane contact site [PMID:23549786]
EXISTENCE:direct assay:GO:1990316 Atg1/ULK1 kinase complex [PMID:26753620]
EXISTENCE:genetic interaction:GO:0060090 molecular adaptor activity [PMID:17295840]
EXISTENCE:genetic interaction:GO:2000786 positive regulation of autophagosome assembly [PMID:18077553]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:15743910]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:15901835]
EXISTENCE:mutant phenotype:GO:0030295 protein kinase activator activity [PMID:10995454]
EXISTENCE:mutant phenotype:GO:0032147 activation of protein kinase activity [PMID:10995454]
EXISTENCE:mutant phenotype:GO:0045772 positive regulation of autophagosome size [PMID:18077553]
EXISTENCE:physical interaction:GO:1990316 Atg1/ULK1 kinase complex [PMID:15743910]
Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
EXISTENCE:direct assay:GO:0000390 spliceosomal complex disassembly [PMID:16880513|PMID:16357217]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:17875666]
EXISTENCE:direct assay:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:16357217]
EXISTENCE:genetic interaction:GO:0000390 spliceosomal complex disassembly [PMID:17875666]
EXISTENCE:mutant phenotype:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:16945917]
EXISTENCE:mutant phenotype:GO:0000390 spliceosomal complex disassembly [PMID:16880513]
EXISTENCE:mutant phenotype:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:16357217]
Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
YLRCTy2-2; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; mutated in S288C
Rho family guanine nucleotide exchange factor TUS1
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:18256282]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:11839800]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:16763112]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:11839800]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:11839800]
EXISTENCE:mutant phenotype:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:16763112]
Guanine nucleotide exchange factor (GEF) that modulates Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:16121259]
hypothetical protein; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
Cytoplasmic hypothetical protein; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:9885246]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:9822583]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:9885246]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:12499356|PMID:21454476]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:9885246]
EXISTENCE:direct assay:GO:0071933 Arp2/3 complex binding [PMID:12499356]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:21454476]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:9885246]
EXISTENCE:mutant phenotype:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:12499356]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:mutant phenotype:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:21454476]
EXISTENCE:physical interaction:GO:0007015 actin filament organization [PMID:9885246]
EXISTENCE:physical interaction:GO:0007017 microtubule-based process [PMID:9885246]
Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:35857496]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23222640|PMID:8544822|PMID:35857496]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:23141540]
EXISTENCE:direct assay:GO:0006386 termination of RNA polymerase III transcription [PMID:35857496]
EXISTENCE:direct assay:GO:0019904 protein domain specific binding [PMID:22286094]
EXISTENCE:direct assay:GO:0033678 5'-3' DNA/RNA helicase activity [PMID:26198638]
EXISTENCE:direct assay:GO:0035649 Nrd1 complex [PMID:16427013]
EXISTENCE:direct assay:GO:0043139 5'-3' DNA helicase activity [PMID:26198638]
EXISTENCE:genetic interaction:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:27179024]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23748379]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:23741394]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9365256]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:17157256]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:22123738]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9365256]
EXISTENCE:mutant phenotype:GO:0016180 snRNA processing [PMID:9365256]
EXISTENCE:mutant phenotype:GO:0019904 protein domain specific binding [PMID:22286094]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:11565036]
EXISTENCE:mutant phenotype:GO:0045005 DNA-templated DNA replication maintenance of fidelity [PMID:23141540]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:27718307]
ATP-dependent 5' to 3' RNA/DNA and DNA helicase; subunit of the exosome-associated Nrd1p complex that mediates 3' end formation of snRNAs, snoRNAs, CUTs and some mRNAs; helicase-independent role in transcription-coupled repair; coordinates replication with transcription, associating with moving forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of juvenile ALS
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:14504273|PMID:18497569]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:14734026]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:14504273]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:17135291]
Peripheral membrane protein required for autophagy and CVT; required for cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095|PMID:11914276]
Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004723 calcium-dependent protein serine/threonine phosphatase activity [PMID:1321337]
EXISTENCE:direct assay:GO:0005955 calcineurin complex [PMID:1321337]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:1651503]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:8798496]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7530227]
Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:25144938]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:25144938]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25144938]
Protein with a potential role in pre-rRNA processing
ARS1232; Autonomously Replicating Sequence
hypothetical protein; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18838542|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:18838542]
Protein that regulates nuclear import of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0006527 arginine catabolic process [PMID:3036506]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:205532]
EXISTENCE:mutant phenotype:GO:0004587 ornithine-oxo-acid transaminase activity [PMID:3036506]
EXISTENCE:mutant phenotype:GO:0006591 ornithine metabolic process [PMID:3036506]
L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress; human homolog OAT complements yeast null mutant
ARS1233; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10369684]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:11720284|PMID:10369684]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15075370]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10449419]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10428970]
EXISTENCE:physical interaction:GO:0005732 sno(s)RNA-containing ribonucleoprotein complex [PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
mitochondrial 54S ribosomal protein YmL4
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:20404317]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:20404317|PMID:3060376]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:20404317|PMID:3060376]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7828914]
Mitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15942868|PMID:14690591]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:19346454]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15942868|PMID:14562095]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15958492]
EXISTENCE:direct assay:GO:0070939 Dsl1/NZR complex [PMID:19151722]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:15958492]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:15242642]
EXISTENCE:mutant phenotype:GO:0032581 ER-dependent peroxisome organization [PMID:19346454]
EXISTENCE:physical interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:15958492]
EXISTENCE:physical interaction:GO:0070939 Dsl1/NZR complex [PMID:15958492]
Component of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; mediates Sey1p-independent homotypic ER fusion; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Ribosomal protein of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:9710643|PMID:16956377]
EXISTENCE:direct assay:GO:0000792 heterochromatin [PMID:20176978]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18195043]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:29795547]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:19099415]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:19099415]
EXISTENCE:direct assay:GO:0005677 chromatin silencing complex [PMID:9122169]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9150138]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:19217406|PMID:27835568]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16581798]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:27122604]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:9501103]
EXISTENCE:mutant phenotype:GO:0008156 negative regulation of DNA replication [PMID:29795547]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16581798|PMID:3297920]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:16908543]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:1913809|PMID:31599702|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:26399229|PMID:27122604]
EXISTENCE:mutant phenotype:GO:0097695 establishment of protein-containing complex localization to telomere [PMID:26218225]
Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
EXISTENCE:mutant phenotype:GO:0006816 calcium ion transport [PMID:21252230]
Putative integral membrane protein with a role in calcium uptake; non-essential protein; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
ARS1234; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000795 synaptonemal complex [PMID:23326245]
EXISTENCE:genetic interaction:GO:0051321 meiotic cell cycle [PMID:22194413]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:23326245]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:23326245]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:22194413]
EXISTENCE:mutant phenotype:GO:0070193 synaptonemal complex organization [PMID:22194413|PMID:23326245]
Protein involved in meiotic crossing over; component of the Synaptonemal Complex (SC) along with Ecm11p; required for the efficient loading of the SC transverse filament protein, Zip1p; promotes SUMOylation of Ecm11p; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor; also detected in peroxisomes
EXISTENCE:direct assay:GO:0045127 N-acetylglucosamine kinase activity [PMID:36216916]
EXISTENCE:mutant phenotype:GO:0045127 N-acetylglucosamine kinase activity [PMID:36216916]
N-acetylglucosamine kinase; GlcNAc kinase that requires a divalent cations and can utilize a broad range of NTPs as phosphoryl donor; phylogenically distinct from known GlcNAc kinases and hexokinases; transcript is upregulated during sporulation and the unfolded protein response; contains a hexokinase-like ATPase domain; NGK1 is not an essential gene
H(+)-transporting V0 sector ATPase subunit d
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:8509410|PMID:26928762]
EXISTENCE:direct assay:GO:0007034 vacuolar transport [PMID:8509410]
EXISTENCE:direct assay:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:8509410]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:8509410]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:8509410]
Subunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:3282992]
Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000412 histone peptidyl-prolyl isomerization [PMID:16959570]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:14981505|PMID:16959570]
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:14981505|PMID:16959570]
EXISTENCE:direct assay:GO:0005527 macrolide binding [PMID:9371805]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14981505|PMID:9371805]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:16846601|PMID:24297734]
EXISTENCE:mutant phenotype:GO:0000415 negative regulation of histone H3-K36 methylation [PMID:16959570]
Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; contains a nucleoplasmin-like fold and can form pentamers
hydroxymethylglutaryl-CoA reductase (NADPH) HMG2
EXISTENCE:direct assay:GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity [PMID:3526336]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:8744950]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:8744950]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8744950]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:3526336]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
EXISTENCE:genetic interaction:GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity [PMID:2828155]
EXISTENCE:physical interaction:GO:0000502 proteasome complex [PMID:27226596]
HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; HMG2 has a paralog, HMG1, that arose from the whole genome duplication; human homolog HMGCR can complement yeast hmg2 mutant
leucine-responsive transcriptional regulator LEU3
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:1439822]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:1439822]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:1439822]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7623798]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1439822|PMID:2183176]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:2823102]
EXISTENCE:mutant phenotype:GO:2001278 positive regulation of leucine biosynthetic process [PMID:2823102]
Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:9537998]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8756677]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:2830483]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:9537998]
GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:9710643]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:17656141|PMID:9710643]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:9087429]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:20523746]
EXISTENCE:physical interaction:GO:0070187 shelterin complex [PMID:23746845]
Protein that binds to the Rap1p C-terminus; inhibits MRX-dependent Tel1p activity by acting directly on Rad50p and discharging its activated ATP-bound state, thereby rendering the MRX complex incompetent for Tel1p activation; acts synergistically with Rif1p to help control telomere length, establish telomeric silencing; deletion results in telomere elongation; RIF2 has paralog ORC4 that arose from the whole genome duplication
Protein with structural similarity to lipid transport protein Vps13p; localizes to multiple membrane contact sites between organelles; non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:25104842]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:25104842]
EXISTENCE:direct assay:GO:1990468 NuA3b histone acetyltransferase complex [PMID:25104842]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:25104842]
Component of the NuA3b histone acetyltransferase complex; regulates interaction between NuA3b and H3K36me3 at the transcribed regions of genes; contains PWWP domain; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
hypothetical protein; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:21785410]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:21785410|PMID:16436507]
EXISTENCE:direct assay:GO:0005823 central plaque of spindle pole body [PMID:16436507]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:21785410]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:16436507]
EXISTENCE:mutant phenotype:GO:0005637 nuclear inner membrane [PMID:21785410]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:16436507]
EXISTENCE:mutant phenotype:GO:0070631 spindle pole body localization [PMID:21785410]
EXISTENCE:physical interaction:GO:0030474 spindle pole body duplication [PMID:16436507]
Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0016255 attachment of GPI anchor to protein [PMID:15075373]
EXISTENCE:mutant phenotype:GO:0042765 GPI-anchor transamidase complex [PMID:15075373]
EXISTENCE:physical interaction:GO:0042765 GPI-anchor transamidase complex [PMID:15075373]
Subunit of the glycosylphosphatidylinositol transamidase complex; involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or the lipid portion of GPI; ortholog of human PIGU; partially complements a class U CHO cell line with a mutation in the PIGU ortholog
ARS1235; Autonomously replicating sequence
Member of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
TEL12R; Telomeric region on the right arm of Chromosome XII; composed of an X element core sequence, X element combinatorial repeats, two distinct regions of telomeric repeats, and two long Y' elements
hypothetical protein with similarity to helicases; YLR462W is within the telomere on the right arm of chromosome XII
hypothetical protein; intron is predicted but not detected experimentally; YLR464W overlaps the verified gene YRF1-4/YLR466W and two dubious ORFs YLR463C and YLR465C
Y' element ATP-dependent helicase protein 1 copy 4
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9837911]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9837911]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
Y' element ATP-dependent helicase protein 1 copy 5