Saccharomyces cerevisiae S288C chromosome XI, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Complete DNA sequence of yeast chromosome XI
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006944.\n\nOn Apr 26, 2011 this sequence version replaced NC_001143.8.\nChromosome XI sequence was verified by resequencing. The sequence\nwas mapped to the previous GenBank records except for a 500 bp\nregion. That region was determined by sequencing a descendant of\nthe S288C strain, AB972, and is present in GenBank as JF740677.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##
TEL11L; Telomeric region on the left arm of Chromosome XI; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21777356]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:direct assay:GO:0072686 mitotic spindle [PMID:21777356]
hypothetical protein; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
ARS1102; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:31322728]
EXISTENCE:direct assay:GO:0000293 ferric-chelate reductase activity [PMID:8164662]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8164662]
EXISTENCE:direct assay:GO:0006826 iron ion transport [PMID:8164662]
EXISTENCE:direct assay:GO:0015677 copper ion import [PMID:9153234]
Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
threo-3-hydroxy-L-aspartate ammonia-lyase SRY1
EXISTENCE:direct assay:GO:0030848 threo-3-hydroxyaspartate ammonia-lyase activity [PMID:12951240]
EXISTENCE:direct assay:GO:0042219 cellular modified amino acid catabolic process [PMID:12951240]
EXISTENCE:mutant phenotype:GO:0030848 threo-3-hydroxyaspartate ammonia-lyase activity [PMID:12951240]
EXISTENCE:mutant phenotype:GO:0042219 cellular modified amino acid catabolic process [PMID:12951240]
3-hydroxyaspartate dehydratase; deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17710650]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006849 plasma membrane pyruvate transport [PMID:17710650]
EXISTENCE:mutant phenotype:GO:0015355 secondary active monocarboxylate transmembrane transporter activity [PMID:17710650]
EXISTENCE:mutant phenotype:GO:0035879 plasma membrane lactate transport [PMID:10198029|PMID:17710650]
EXISTENCE:mutant phenotype:GO:0097079 selenite:proton symporter activity [PMID:20861301]
EXISTENCE:mutant phenotype:GO:0097080 plasma membrane selenite transport [PMID:20861301]
Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
EXISTENCE:direct assay:GO:0004152 dihydroorotate dehydrogenase activity [PMID:1511880]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1409592]
EXISTENCE:direct assay:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:10871048]
EXISTENCE:mutant phenotype:GO:0004152 dihydroorotate dehydrogenase activity [PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:5651325|PMID:4550660]
Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0017168 5-oxoprolinase (ATP-hydrolyzing) activity [PMID:20402795]
EXISTENCE:mutant phenotype:GO:0006749 glutathione metabolic process [PMID:20349993]
EXISTENCE:mutant phenotype:GO:0017168 5-oxoprolinase (ATP-hydrolyzing) activity [PMID:20402795]
5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11390651]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11390651]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:11390651]
Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:16428438|PMID:21070969]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:23525333]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:12399380]
EXISTENCE:mutant phenotype:GO:0010992 ubiquitin recycling [PMID:18508771|PMID:19805280|PMID:7615550]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:20508643]
EXISTENCE:mutant phenotype:GO:0043130 ubiquitin binding [PMID:16428438]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:2111732|PMID:23525333]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579|PMID:21070972]
WD-repeat protein involved in Ub-mediated proteolysis; K48-, K29-specific Ub chain binding protein involved in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon replication stress
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:15657391|PMID:8314848]
EXISTENCE:direct assay:GO:0004438 phosphatidylinositol-3-phosphate phosphatase activity [PMID:10625610]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11514624|PMID:15657391|PMID:8314848]
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:22553352]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:30785834]
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:direct assay:GO:0043812 phosphatidylinositol-4-phosphate phosphatase activity [PMID:10625610]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10625610]
EXISTENCE:direct assay:GO:0052629 phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity [PMID:10625610]
EXISTENCE:genetic interaction:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10625610]
EXISTENCE:mutant phenotype:GO:0000139 Golgi membrane [PMID:15657391]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:11514624|PMID:11792713|PMID:15657391]
EXISTENCE:mutant phenotype:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10625610|PMID:22553352]
EXISTENCE:mutant phenotype:GO:0061909 autophagosome-lysosome fusion [PMID:32693712]
EXISTENCE:mutant phenotype:GO:0072517 host cell viral assembly compartment [PMID:32269127]
Phosphatidylinositol phosphate phosphatase; role in early and medial Golgi PtdIns4P hydrolysis; regulated by Vps74p interaction; ER localized transmembrane protein which cycles through the Golgi; role in protein trafficking, processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis by modulating PtdIns(4)P metabolism; PtdIns4P PPase activity is crucial for autophagosome-vacuole fusion during autophagy; human homolog SACM1L complements autophagy defects of the null
bifunctional anthranilate synthase/indole-3-glycerol-phosphate synthase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:genetic interaction:GO:0004049 anthranilate synthase activity [PMID:3881257]
EXISTENCE:genetic interaction:GO:0004425 indole-3-glycerol-phosphate synthase activity [PMID:6323449]
EXISTENCE:mutant phenotype:GO:0000162 tryptophan biosynthetic process [PMID:10790693]
EXISTENCE:mutant phenotype:GO:0004425 indole-3-glycerol-phosphate synthase activity [PMID:3881257]
EXISTENCE:physical interaction:GO:0005950 anthranilate synthase complex [PMID:3881257]
Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position C2337
EXISTENCE:direct assay:GO:0004839 ubiquitin activating enzyme activity [PMID:1989885]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7629121|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7629121|PMID:22842922]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:1989885]
Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
ATP-binding cassette a-factor transporter STE6
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8045256]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7679674]
EXISTENCE:direct assay:GO:0015440 ABC-type peptide transporter activity [PMID:8626301|PMID:11389139]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:7679674|PMID:12374868]
EXISTENCE:mutant phenotype:GO:0000770 peptide pheromone export [PMID:2569166]
EXISTENCE:mutant phenotype:GO:0015440 ABC-type peptide transporter activity [PMID:8626301|PMID:11389139]
Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells; human homolog ABCB1 mediates multidrug resistance in many chemotherapy-resistant tumors by effluxing toxic compounds from the cell
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis; correct folding and efficient aminoacylation require Lhp1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0006397 mRNA processing [PMID:9339340]
EXISTENCE:direct assay:GO:0017004 cytochrome complex assembly [PMID:9339340]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:16118200|PMID:9339340]
EXISTENCE:mutant phenotype:GO:0017004 cytochrome complex assembly [PMID:9339340]
EXISTENCE:mutant phenotype:GO:0048255 mRNA stabilization [PMID:16118200]
Protein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17634286]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:17431397]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:17634286]
Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18045534]
EXISTENCE:direct assay:GO:0016363 nuclear matrix [PMID:8366091]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:9774653]
EXISTENCE:genetic interaction:GO:0071528 tRNA re-export from nucleus [PMID:20032305]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:9802895]
Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm; deletion mutation extends replicative lifespan, as does exclusion of Los1p from the nucleus in response to caloric restriction
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:22890846]
EXISTENCE:direct assay:GO:0008190 eukaryotic initiation factor 4E binding [PMID:10848587]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:26777405]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:10848587]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:22890846]
EXISTENCE:mutant phenotype:GO:0006402 mRNA catabolic process [PMID:22890846]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:10848587]
eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
ARS1103; Autonomously Replicating Sequence
phosphatidylinositol kinase-related protein kinase TOR2
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:8846782]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:16055732|PMID:22307609]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10973982]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:9461583]
EXISTENCE:genetic interaction:GO:0035025 positive regulation of Rho protein signal transduction [PMID:9038344]
EXISTENCE:genetic interaction:GO:2001108 positive regulation of Rho guanyl-nucleotide exchange factor activity [PMID:9038344]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:16055732]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:9475724]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:12719473|PMID:8186460]
EXISTENCE:mutant phenotype:GO:0035025 positive regulation of Rho protein signal transduction [PMID:9038344]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:10198052]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:14593073]
EXISTENCE:mutant phenotype:GO:1905356 regulation of snRNA pseudouridine synthesis [PMID:27268497]
EXISTENCE:mutant phenotype:GO:2001108 positive regulation of Rho guanyl-nucleotide exchange factor activity [PMID:9038344]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12631735]
EXISTENCE:physical interaction:GO:0031931 TORC1 complex [PMID:12408816]
EXISTENCE:physical interaction:GO:0031932 TORC2 complex [PMID:12408816]
PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates plasma membrane (PM) homeostasis and cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; PM retention is dependent on Avo3p; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:30842360]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9023541]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:9813355]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:9459307]
Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; may also function as a mannosylphosphate transferase; expression increases in late-logarithmic and stationary growth phases; coding sequence contains length polymorphisms in different strains; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
putative serine/threonine protein kinase PTK1
EXISTENCE:genetic interaction:GO:0000296 spermine transport [PMID:7488221]
Putative serine/threonine protein kinase; regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein; PTK1 has a paralog, PTK2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:14562095]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:16911527]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:direct assay:GO:0043335 protein unfolding [PMID:29321502]
EXISTENCE:genetic interaction:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:mutant phenotype:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:16007078]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:15634331]
AAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis; mutations in human PEX1 can lead to severe peroxisomal disorders and early death
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:15723044]
EXISTENCE:direct assay:GO:0000421 autophagosome membrane [PMID:33025734]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11959998|PMID:33025734]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18541004]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:18541004]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11959998]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9211930]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:14685280]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:11959998]
EXISTENCE:direct assay:GO:0061909 autophagosome-lysosome fusion [PMID:33025734]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9211930]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:10591633]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10385523]
Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within Golgi, endocytic trafficking to vacuole, and vacuolar fusion; membrane localization due to prenylation at carboxy-terminus; targeted to vacuole via AP-3 pathway; human homolog YKT6 can complement yeast ykt6 mutant
EXISTENCE:direct assay:GO:0003756 protein disulfide isomerase activity [PMID:25451030]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15364952|PMID:15620710]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:15359280]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:22990235|PMID:25451030]
EXISTENCE:direct assay:GO:0016972 thiol oxidase activity [PMID:25451030]
EXISTENCE:direct assay:GO:0051537 2 iron, 2 sulfur cluster binding [PMID:23834247]
EXISTENCE:mutant phenotype:GO:0016491 oxidoreductase activity [PMID:22990235]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:15359280|PMID:15364952|PMID:17336303|PMID:27343349]
EXISTENCE:physical interaction:GO:0045041 protein import into mitochondrial intermembrane space [PMID:15989955]
Import and assembly protein in mitochondrial intermembrane space; component of MIA pathway which mediates import and oxidative folding of substrates including small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p via a chaperone-like activity; forms a dimer that binds iron-sulfur cluster in vitro
EXISTENCE:direct assay:GO:0004829 threonine-tRNA ligase activity [PMID:21321019]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2999113|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0070159 mitochondrial threonyl-tRNA aminoacylation [PMID:21321019]
EXISTENCE:mutant phenotype:GO:0004829 threonine-tRNA ligase activity [PMID:2999113]
Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro; lacks a typical editing domain, but has pre-transfer editing activity stimulated by the unusual tRNA-Thr
type 1 protein phosphatase-activating protein SDS22
EXISTENCE:direct assay:GO:0000164 protein phosphatase type 1 complex [PMID:10775415]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11801737|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:genetic interaction:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:17142459]
EXISTENCE:genetic interaction:GO:0072542 protein phosphatase activator activity [PMID:11801737|PMID:18172024]
EXISTENCE:mutant phenotype:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:17142459]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11801737]
EXISTENCE:mutant phenotype:GO:0051457 maintenance of protein location in nucleus [PMID:11801737]
Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
EXISTENCE:mutant phenotype:GO:0009107 lipoate biosynthetic process [PMID:9187370]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:27540631]
EXISTENCE:physical interaction:GO:0099128 mitochondrial iron-sulfur cluster assembly complex [PMID:27540631]
Mitochondrial matrix acyl carrier protein; involved in biosynthesis of octanoate, which is a precursor to lipoic acid; coordinates mitochondrial fatty acid and iron sulfur cluster metabolism; activated by phosphopantetheinylation catalyzed by Ppt2p
2-(3-amino-3-carboxypropyl)histidine synthase
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:8406038|PMID:15485916]
Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); forms a complex with Dph1p that catalyzes the first step of diphthamide biosynthesis
EXISTENCE:direct assay:GO:0004723 calcium-dependent protein serine/threonine phosphatase activity [PMID:1321337]
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:1321337]
EXISTENCE:direct assay:GO:0005955 calcineurin complex [PMID:1321337]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:1321337]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:8798496]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7530227]
Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12972564]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12972564]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12972564]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:11259593]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:12972564]
Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis; interacts with Sog2p; allosterically activates Kic1p; localizes to sites of polarized growth during budding and during the mating response
ATP-binding cassette long-chain fatty acid transporter PXA2
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27663510]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:9395310|PMID:35127709]
EXISTENCE:direct assay:GO:0015867 ATP transport [PMID:35127709]
EXISTENCE:direct assay:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:9395310|PMID:35127709]
EXISTENCE:direct assay:GO:1902001 fatty acid transmembrane transport [PMID:9395310]
Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transportesr ABCD1 and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant
ARS1104; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:mutant phenotype:GO:0015909 long-chain fatty acid transport [PMID:23349476]
Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:17434126]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:17434126|PMID:31439809]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:22956913]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:29212662]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:29212662]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:7865887|PMID:9774696]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10952996]
EXISTENCE:physical interaction:GO:0042272 nuclear RNA export factor complex [PMID:9774696]
mRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
DNA-binding transcription repressor ASH1
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:11171979]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:19841732]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8625408|PMID:9566907|PMID:8625409]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:8625408|PMID:8625409]
EXISTENCE:direct assay:GO:0033698 Rpd3L complex [PMID:16286007|PMID:16314178|PMID:19040720]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:direct assay:GO:1900477 negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter [PMID:19841732]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8625408|PMID:8625409]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:11171979]
EXISTENCE:mutant phenotype:GO:1900461 positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter [PMID:11046133]
EXISTENCE:mutant phenotype:GO:1900477 negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter [PMID:19841732]
Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0004586 ornithine decarboxylase activity [PMID:2681188]
EXISTENCE:mutant phenotype:GO:0009446 putrescine biosynthetic process [PMID:7929015]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
ARS1125; Autonomously Replicating Sequence
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
hypothetical protein; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
tetrafunctional fatty acid synthase subunit FAS1
EXISTENCE:direct assay:GO:0004313 [acyl-carrier-protein] S-acetyltransferase activity [PMID:374077]
EXISTENCE:direct assay:GO:0004314 [acyl-carrier-protein] S-malonyltransferase activity [PMID:365179|PMID:374077]
EXISTENCE:direct assay:GO:0004319 enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity [PMID:374077]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:17803462]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:6025308]
EXISTENCE:direct assay:GO:0005835 fatty acid synthase complex [PMID:65153]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:365179]
EXISTENCE:direct assay:GO:0019171 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity [PMID:374077]
EXISTENCE:mutant phenotype:GO:0004312 fatty acid synthase activity [PMID:1100391|PMID:65153]
EXISTENCE:mutant phenotype:GO:0004313 [acyl-carrier-protein] S-acetyltransferase activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0004314 [acyl-carrier-protein] S-malonyltransferase activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0004319 enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0016409 palmitoyltransferase activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0019171 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity [PMID:1100391]
EXISTENCE:mutant phenotype:GO:0042759 long-chain fatty acid biosynthetic process [PMID:1735446|PMID:4127627]
Beta subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
ribose phosphate diphosphokinase subunit PRS1
EXISTENCE:direct assay:GO:0002189 ribose phosphate diphosphokinase complex [PMID:15280369]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11101685|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:genetic interaction:GO:0004749 ribose phosphate diphosphokinase activity [PMID:15280369]
EXISTENCE:genetic interaction:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:15280369]
EXISTENCE:mutant phenotype:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:10212224]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:15470112]
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; plays a key role in cell wall integrity (CWI) pathway; one of five related enzymes, which are active as heteromultimeric complexes; missense mutations in human homolog PRPS1 are associated with neuropathic Arts syndrome and Charcot-Marie Tooth (CMTX5) disease
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23268442]
EXISTENCE:genetic interaction:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23268442]
Ribosomal 60S subunit protein L17A; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:31073031]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:31073031]
EXISTENCE:direct assay:GO:0048211 Golgi vesicle docking [PMID:31073031]
EXISTENCE:genetic interaction:GO:0000139 Golgi membrane [PMID:12429822]
EXISTENCE:genetic interaction:GO:0048193 Golgi vesicle transport [PMID:12429822]
Golgi membrane protein with similarity to mammalian CASP; packaged into COPII vesicles for trafficking between ER and Golgi; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
EXISTENCE:direct assay:GO:0000755 cytogamy [PMID:17369365]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:3006051]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3006051]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:17369365]
EXISTENCE:mutant phenotype:GO:0004933 mating-type a-factor pheromone receptor activity [PMID:3006051]
Receptor for a factor pheromone; couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:26387955]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26387955]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:26387955]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:26387955]
EXISTENCE:direct assay:GO:1990877 FNIP-folliculin RagC/D GAP [PMID:26387955]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:26387955]
Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10856230]
EXISTENCE:mutant phenotype:GO:0005385 zinc ion transmembrane transporter activity [PMID:10856230]
EXISTENCE:mutant phenotype:GO:0006829 zinc ion transport [PMID:10856230]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:10856230]
Vacuolar membrane zinc transporter; transports zinc from storage in vacuole to cytoplasm when needed; transcription induced under conditions of zinc deficiency; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15668236]
EXISTENCE:mutant phenotype:GO:0015203 polyamine transmembrane transporter activity [PMID:15668236]
EXISTENCE:mutant phenotype:GO:0015846 polyamine transport [PMID:15668236]
Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0030623 U5 snRNA binding [PMID:9848662]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:genetic interaction:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:15911574]
EXISTENCE:mutant phenotype:GO:0000244 spliceosomal tri-snRNP complex assembly [PMID:16540695]
EXISTENCE:mutant phenotype:GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) [PMID:16540695]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9233818]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:9233818]
GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10849420]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:21822217]
EXISTENCE:mutant phenotype:GO:0000280 nuclear division [PMID:15515129]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:10849420]
Protein required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:11914276]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:mutant phenotype:GO:0006508 proteolysis [PMID:11595789]
Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
mitochondrial 54S ribosomal protein YmL38/YmL34
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
putative serine/threonine protein kinase KKQ8
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:14562095]
Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
mitochondrial 54S ribosomal protein MRP49
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:1544898]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:1544898]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:1544898]
Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
cAMP-dependent protein kinase catalytic subunit TPK3
EXISTENCE:direct assay:GO:0000932 P-body [PMID:22899713]
EXISTENCE:direct assay:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19804918|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19804918|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:22899713]
EXISTENCE:genetic interaction:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:genetic interaction:GO:0005952 cAMP-dependent protein kinase complex [PMID:3036373]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:19897478]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:3036373]
EXISTENCE:genetic interaction:GO:0010607 negative regulation of cytoplasmic mRNA processing body assembly [PMID:21925385]
EXISTENCE:genetic interaction:GO:0010737 protein kinase A signaling [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:19897478]
EXISTENCE:mutant phenotype:GO:2000766 negative regulation of cytoplasmic translation [PMID:22899713]
cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
mannose-ethanolamine phosphotransferase MCD4
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:10514566]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10069808|PMID:26928762]
EXISTENCE:direct assay:GO:0006506 GPI anchor biosynthetic process [PMID:12023081]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:12023081]
EXISTENCE:direct assay:GO:0015867 ATP transport [PMID:12807869]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:17311586|PMID:16704983]
EXISTENCE:mutant phenotype:GO:0051377 mannose-ethanolamine phosphotransferase activity [PMID:17311586|PMID:16704983]
Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:9301021]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:16278454]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460|PMID:9192695]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:11238901]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10407261]
O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:10407261]
O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication
type II protein arginine methyltransferase
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
Protein kinase; implicated in Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p; KDX1 has a paralog, SLT2, that arose from the whole genome duplication
ARS1106; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:20818391]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19159085|PMID:14562095]
EXISTENCE:direct assay:GO:0008023 transcription elongation factor complex [PMID:20818391]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16260625]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16260625]
EXISTENCE:mutant phenotype:GO:0045815 transcription initiation-coupled chromatin remodeling [PMID:16260625]
Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:10887154]
EXISTENCE:direct assay:GO:0030346 protein phosphatase 2B binding [PMID:10887154]
EXISTENCE:mutant phenotype:GO:0008597 calcium-dependent protein serine/threonine phosphatase regulator activity [PMID:10887154]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:17954914]
Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:383482]
EXISTENCE:direct assay:GO:0030287 cell wall-bounded periplasmic space [PMID:383482]
EXISTENCE:direct assay:GO:0043171 peptide catabolic process [PMID:6352682]
EXISTENCE:direct assay:GO:0070006 metalloaminopeptidase activity [PMID:6352682]
EXISTENCE:mutant phenotype:GO:0043171 peptide catabolic process [PMID:3286257]
Aminopeptidase yscII; may have role in obtaining leucine from dipeptide substrates; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; APE2 has a paralog, AAP1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:3533916]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:9271380]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
mitochondrial 37S ribosomal protein RSM22
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent RNA methyltransferase
Signal recognition particle receptor subunit beta
EXISTENCE:curator inference:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:7844142]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9679135]
EXISTENCE:mutant phenotype:GO:0005047 signal recognition particle binding [PMID:9679135]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:9679135]
EXISTENCE:physical interaction:GO:0005785 signal recognition particle receptor complex [PMID:9679135]
Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0004619 phosphoglycerate mutase activity [PMID:1386023]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:3332961]
EXISTENCE:mutant phenotype:GO:0004619 phosphoglycerate mutase activity [PMID:3033435]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:3033435]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:11015729]
Tetrameric phosphoglycerate mutase; mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
EXISTENCE:direct assay:GO:0046496 nicotinamide nucleotide metabolic process [PMID:21994945]
EXISTENCE:direct assay:GO:0047453 ATP-dependent NAD(P)H-hydrate dehydratase activity [PMID:21994945]
Widely-conserved NADHX dehydratase; converts (S)-NADHX to NADH in ATP-dependent manner; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; downstream intergenic region drives antisense expression and mediates coordinated regulation of YKL151C and GPM1 phosphoglycerate mutase; protein abundance increases in response to DNA replication stress; homolog of Carkd in mammals and C-terminus of YjeF in E.coli
EXISTENCE:direct assay:GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H [PMID:8001120]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:8001120|PMID:16407407]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:8001120]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:10622712]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11420140]
Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:1850323]
EXISTENCE:direct assay:GO:0006401 RNA catabolic process [PMID:1850323]
EXISTENCE:direct assay:GO:0008419 RNA lariat debranching enzyme activity [PMID:1850323]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:24919400]
EXISTENCE:mutant phenotype:GO:0006401 RNA catabolic process [PMID:1850323]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0008419 RNA lariat debranching enzyme activity [PMID:1850323]
EXISTENCE:mutant phenotype:GO:0016074 sno(s)RNA metabolic process [PMID:9740128]
EXISTENCE:mutant phenotype:GO:0032197 transposition, RNA-mediated [PMID:10652222]
RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies; human homolog DBR1 can complement yeast dbr1 null mutant
succinate dehydrogenase flavoprotein subunit SDH1
EXISTENCE:curator inference:GO:0006099 tricarboxylic acid cycle [PMID:13445213]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2826438|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005749 mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) [PMID:9822678]
EXISTENCE:direct assay:GO:0006121 mitochondrial electron transport, succinate to ubiquinone [PMID:2826438]
EXISTENCE:direct assay:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:13445213]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:1939170]
EXISTENCE:direct assay:GO:0050660 flavin adenine dinucleotide binding [PMID:18279395]
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921]
EXISTENCE:genetic interaction:GO:0045333 cellular respiration [PMID:16232921|PMID:9730279]
EXISTENCE:mutant phenotype:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921|PMID:197391|PMID:9730279]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:16232921|PMID:9730279]
EXISTENCE:mutant phenotype:GO:0050660 flavin adenine dinucleotide binding [PMID:1939170]
Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; mutations in human ortholog SDHA are associated with Leigh syndrome and predisposition to several cancers
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:27246536]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:genetic interaction:GO:0005302 L-tyrosine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0015186 L-glutamine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0015188 L-isoleucine transmembrane transporter activity [PMID:11274162]
EXISTENCE:genetic interaction:GO:0032974 amino acid transmembrane export from vacuole [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0005302 L-tyrosine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015186 L-glutamine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015188 L-isoleucine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015824 proline transport [PMID:27246536]
EXISTENCE:mutant phenotype:GO:0032974 amino acid transmembrane export from vacuole [PMID:11274162]
Vacuolar transporter; exports large neutral amino acids from vacuole; targeted to vacuole via AP-3 pathway; member of family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
proteasome regulatory particle base subunit RPT1
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:9741626]
EXISTENCE:direct assay:GO:0045732 positive regulation of protein catabolic process [PMID:15135049]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:19843524]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19412160]
EXISTENCE:physical interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15135049]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:10688918]
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
DNA-directed RNA polymerase III subunit RPC25
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:mutant phenotype:GO:0006384 transcription initiation at RNA polymerase III promoter [PMID:15612920]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:15612920|PMID:8065349]
RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16888326]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16888326|PMID:14562095]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:16738661|PMID:15167894]
EXISTENCE:direct assay:GO:0031902 late endosome membrane [PMID:16732272]
EXISTENCE:direct assay:GO:0071986 Ragulator complex [PMID:16732272]
EXISTENCE:genetic interaction:GO:0000056 ribosomal small subunit export from nucleus [PMID:16888326]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:16888326]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15611164]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:15611164]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; required for ribosomal small subunit export from nucleus; required for growth at low temperature
hypothetical protein; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
succinate dehydrogenase cytochrome b subunit SDH3
EXISTENCE:direct assay:GO:0005749 mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) [PMID:22152483|PMID:9822678]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:22152483]
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:18028869]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:22152483]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:16232921]
Subunit of succinate dehydrogenase and of TIM22 translocase; functions as cytochrome b subunit of succinate dehydrogenase, which couples oxidation of succinate to transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex; SDH3 has a paralog, SHH3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004771 sterol esterase activity [PMID:15713625]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:15713625|PMID:24868093|PMID:10515935]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15713625]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:15713625]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:10515935]
Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
cyclin-dependent serine/threonine protein kinase CTK1
EXISTENCE:direct assay:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:7565723]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9110987]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15520468]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15520468]
EXISTENCE:direct assay:GO:0032786 positive regulation of DNA-templated transcription, elongation [PMID:9110987]
EXISTENCE:direct assay:GO:0070692 CTDK-1 complex [PMID:7565723]
EXISTENCE:genetic interaction:GO:0031124 mRNA 3'-end processing [PMID:12504017]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:25416238]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:12504017]
EXISTENCE:mutant phenotype:GO:0045903 positive regulation of translational fidelity [PMID:17545469]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15520468]
Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:15664196|PMID:16777964]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:17620411]
EXISTENCE:direct assay:GO:0031116 positive regulation of microtubule polymerization [PMID:15664196]
EXISTENCE:direct assay:GO:0042729 DASH complex [PMID:15640796|PMID:15664196]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:17620411|PMID:20479465]
EXISTENCE:direct assay:GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore [PMID:20479465|PMID:20479468]
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
mitochondrial 54S ribosomal protein YmL31
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:18443040]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19703468]
EXISTENCE:genetic interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:23198688]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:18443040|PMID:23198688]
Copper-binding protein of the mitochondrial intermembrane space; evolutionarily conserved; may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif
EXISTENCE:direct assay:GO:0030121 AP-1 adaptor complex [PMID:10564262]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:10564262]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:17003107]
Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:8831696]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8035833]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:10332043]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:8831696]
EXISTENCE:mutant phenotype:GO:0006627 protein processing involved in protein targeting to mitochondrion [PMID:8035833]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10332043]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:21525245]
Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
Mitochondrial hypothetical protein; not required for growth of cells lacking the mitochondrial genome; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; YKL133C has a paralog, MGR3, that arose from the whole genome duplication
Putative dihydrofolate synthetase; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; RMA1 has a paralog, FOL3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11101531]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8625407]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16890529|PMID:24056370]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11032818]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:24056370]
EXISTENCE:direct assay:GO:0008298 intracellular mRNA localization [PMID:11032818|PMID:24056370]
EXISTENCE:direct assay:GO:1990825 sequence-specific mRNA binding [PMID:28092367]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:8625407]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:28092367]
RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER
ARS1126; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:11604421]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:18177206]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant; localizes to cytosol an peroxisomes
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8385141]
EXISTENCE:genetic interaction:GO:0004614 phosphoglucomutase activity [PMID:8119301]
EXISTENCE:genetic interaction:GO:0005978 glycogen biosynthetic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0005992 trehalose biosynthetic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0006011 UDP-glucose metabolic process [PMID:9252577]
EXISTENCE:genetic interaction:GO:0019255 glucose 1-phosphate metabolic process [PMID:8119301]
EXISTENCE:genetic interaction:GO:0019388 galactose catabolic process [PMID:8119301]
EXISTENCE:genetic interaction:GO:0051156 glucose 6-phosphate metabolic process [PMID:8119301]
Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:19966303|PMID:22080611|PMID:29352143]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10825204|PMID:12221112]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10825204]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10825204]
EXISTENCE:genetic interaction:GO:0006650 glycerophospholipid metabolic process [PMID:25258318]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:12221112]
EXISTENCE:genetic interaction:GO:0070941 eisosome assembly [PMID:18296441]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:10825204|PMID:26274562]
EXISTENCE:mutant phenotype:GO:0006650 glycerophospholipid metabolic process [PMID:25258318]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:12221112]
EXISTENCE:mutant phenotype:GO:0061093 negative regulation of phospholipid translocation [PMID:19966303]
EXISTENCE:mutant phenotype:GO:0071311 cellular response to acetate [PMID:27671892]
EXISTENCE:mutant phenotype:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:22080611]
S/T protein kinase; required for PM lipid and protein homeostasis; involved in sphingolipid-mediated and cell integrity signaling pathways and endocytosis; phosphorylates and downregulates Fpk1p; phosphorylates and inactivates Orm1p/2p when sphingolipid synthesis is compromised; role in TORC1/2-dependent Rps6a/b phosphorylation; phosphorylated and activated by TORC2 and by Pkh1/2p in the presence of phytosphingosine; human homolog SGK1 complements the null and inviability of a ypk1 ypk2 mutant
rDNA-binding RNA polymerase I transcriptional factor
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:23393135]
EXISTENCE:direct assay:GO:0001042 RNA polymerase I core binding [PMID:11032814]
EXISTENCE:direct assay:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:11032814]
EXISTENCE:direct assay:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:11032814]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19416370]
EXISTENCE:genetic interaction:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:11032814]
EXISTENCE:mutant phenotype:GO:0006361 transcription initiation at RNA polymerase I promoter [PMID:8670901]
EXISTENCE:physical interaction:GO:0001042 RNA polymerase I core binding [PMID:11032814]
EXISTENCE:physical interaction:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:11032814]
EXISTENCE:physical interaction:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:11032814]
Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:17287526]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17287526]
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:30361468|PMID:18367543]
EXISTENCE:mutant phenotype:GO:1990756 ubiquitin ligase-substrate adaptor activity [PMID:30361468]
Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases
signal recognition particle subunit SRP21
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:7925282]
EXISTENCE:direct assay:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:10921896]
Subunit of the signal recognition particle (SRP); SRP functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
ARS1127; Autonomously Replicating Sequence
hypothetical protein; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10428783]
EXISTENCE:direct assay:GO:0008272 sulfate transport [PMID:10428783]
EXISTENCE:direct assay:GO:0015116 sulfate transmembrane transporter activity [PMID:10428783]
EXISTENCE:direct assay:GO:0015131 oxaloacetate transmembrane transporter activity [PMID:10428783]
EXISTENCE:direct assay:GO:0015729 oxaloacetate transport [PMID:10428783]
EXISTENCE:direct assay:GO:0034658 isopropylmalate transmembrane transporter activity [PMID:18682385]
EXISTENCE:direct assay:GO:0034659 isopropylmalate transport [PMID:18682385]
EXISTENCE:direct assay:GO:1901239 malonate(1-) transmembrane transporter activity [PMID:10428783]
Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9325326]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:1628805]
EXISTENCE:mutant phenotype:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:9325326]
EXISTENCE:physical interaction:GO:1990871 Vma12-Vma22 assembly complex [PMID:9660861]
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10207098]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10207098]
EXISTENCE:direct assay:GO:0032212 positive regulation of telomere maintenance via telomerase [PMID:17389357]
EXISTENCE:direct assay:GO:0043392 negative regulation of DNA binding [PMID:23022381]
EXISTENCE:direct assay:GO:0051972 regulation of telomerase activity [PMID:17389357]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9632755]
EXISTENCE:mutant phenotype:GO:0032212 positive regulation of telomere maintenance via telomerase [PMID:17389357]
EXISTENCE:mutant phenotype:GO:0051087 chaperone binding [PMID:9632755]
EXISTENCE:mutant phenotype:GO:0051972 regulation of telomerase activity [PMID:17389357]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:9632755]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:9632755]
Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11337509]
EXISTENCE:genetic interaction:GO:0031138 negative regulation of conjugation with cellular fusion [PMID:11337509]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11337509]
EXISTENCE:mutant phenotype:GO:0031138 negative regulation of conjugation with cellular fusion [PMID:11337509]
Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor
DNA-(apurinic or apyrimidinic site) lyase APN1
EXISTENCE:direct assay:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:12767225]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7690756]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11238901]
EXISTENCE:direct assay:GO:0006284 base-excision repair [PMID:9295360]
EXISTENCE:direct assay:GO:0008081 phosphoric diester hydrolase activity [PMID:3056935]
EXISTENCE:direct assay:GO:0008311 double-stranded DNA 3'-5' exodeoxyribonuclease activity [PMID:16024777]
EXISTENCE:direct assay:GO:0017005 3'-tyrosyl-DNA phosphodiesterase activity [PMID:12397185]
EXISTENCE:mutant phenotype:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:1693433]
Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19699691|PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19699691]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15342630]
EXISTENCE:direct assay:GO:0008409 5'-3' exonuclease activity [PMID:9166764]
EXISTENCE:direct assay:GO:0017108 5'-flap endonuclease activity [PMID:11825897]
EXISTENCE:direct assay:GO:0043137 DNA replication, removal of RNA primer [PMID:16837458]
EXISTENCE:genetic interaction:GO:0006286 base-excision repair, base-free sugar-phosphate removal [PMID:9927726]
EXISTENCE:genetic interaction:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:16079237]
EXISTENCE:mutant phenotype:GO:0006286 base-excision repair, base-free sugar-phosphate removal [PMID:9927726]
EXISTENCE:mutant phenotype:GO:0007534 gene conversion at mating-type locus [PMID:10025407]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:16079237]
5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000113 nucleotide-excision repair factor 4 complex [PMID:10601031]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7663308|PMID:27016735]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:7663308]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:3281162|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15522095]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:11756546]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:10684934|PMID:11713306]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:3275867|PMID:7663308|PMID:19111667]
EXISTENCE:direct assay:GO:0044374 sequence-specific DNA binding, bending [PMID:8206955]
EXISTENCE:direct assay:GO:0070911 global genome nucleotide-excision repair [PMID:15177043]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:10684934|PMID:11713306]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15192094]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:15522095]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16908533|PMID:8582634]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15192094]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:10601031]
DNA binding protein with possible chromatin-reorganizing activity; involved in transcriptional activation, gene silencing, and DNA replication and repair
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:15772087]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12139626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12139626]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:15772087]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
EXISTENCE:direct assay:GO:0016602 CCAAT-binding factor complex [PMID:2676721]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11423663]
EXISTENCE:mutant phenotype:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:2676721]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:2123465]
EXISTENCE:mutant phenotype:GO:0043457 regulation of cellular respiration [PMID:2676721]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11423663]
Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:21109535]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:21109535]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:20231317]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:17167415|PMID:17167417]
EXISTENCE:genetic interaction:GO:0031261 DNA replication preinitiation complex [PMID:20231317]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0003688 DNA replication origin binding [PMID:21109535]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:21109535]
EXISTENCE:mutant phenotype:GO:0031333 negative regulation of protein-containing complex assembly [PMID:24307213]
EXISTENCE:mutant phenotype:GO:0033314 mitotic DNA replication checkpoint signaling [PMID:10097122]
EXISTENCE:mutant phenotype:GO:0071163 DNA replication preinitiation complex assembly [PMID:20231317]
Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
putative short-chain dehydrogenase/reductase
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:31115629]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:31115629]
EXISTENCE:mutant phenotype:GO:1901426 response to furfural [PMID:31115629]
NADH-dependent aldehyde reductase; involved in the detoxification of acetaldehyde, glycolaldehyde, furfural, formaldehyde and propionaldehyde; localizes to the endoplasmic reticulum; member of the classical short-chain dehydrogenase/reductase (SDR) family
hypothetical protein; identified by homology to uncharacterized proteins in other fungi
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961|PMID:22010850]
EXISTENCE:mutant phenotype:GO:1901605 alpha-amino acid metabolic process [PMID:33397945]
Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
Eisosome component; likely plays only a minor role in eisosome assembly; shown to interact with Seg1p by affinity purification and mass spec; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; similar to A. gossypii SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication
glutamine--fructose-6-phosphate transaminase (isomerizing) GFA1
EXISTENCE:direct assay:GO:0004360 glutamine-fructose-6-phosphate transaminase (isomerizing) activity [PMID:2656689]
EXISTENCE:genetic interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:11895440]
Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
Novel ORF identified by enrichment-based proteomics
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:1400574]
EXISTENCE:direct assay:GO:0034270 Cvt complex [PMID:9151668|PMID:9412464|PMID:20659891]
EXISTENCE:direct assay:GO:0070006 metalloaminopeptidase activity [PMID:3882418]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:18725539]
Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to peptidase family M18; often used as marker in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway
hypothetical protein; conserved across S. cerevisiae strains; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:27053666|PMID:9925642]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:9925642|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11408575]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10805747|PMID:15282802]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:10490630]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:11408575]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:23042131]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:9925642]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:8647431]
EXISTENCE:mutant phenotype:GO:1902935 protein localization to septin ring [PMID:27053666]
EXISTENCE:physical interaction:GO:0000144 cellular bud neck septin ring [PMID:9925642]
Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p
EXISTENCE:direct assay:GO:1990578 perinuclear endoplasmic reticulum membrane [PMID:25454947]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:25454947]
EXISTENCE:mutant phenotype:GO:0042500 aspartic endopeptidase activity, intramembrane cleaving [PMID:25454947]
EXISTENCE:mutant phenotype:GO:0051603 proteolysis involved in protein catabolic process [PMID:25454947]
Intramembrane aspartyl protease of the perinuclear ER membrane; acts in a branch of ER-associated degradation (ERAD) that degrades functional proteins rather than misfolded proteins; regulates abundance of high-affinity plasma membrane transporters, such as Ctr1p and Zrt1p, during the starvation response; has a presenilin fold; member of the GxGD family of intramembrane proteases; closest human homolog is signal peptide peptidase (SPP)
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
hypothetical protein; mtc2 is synthetically sick with cdc13-1
hypothetical protein; conserved across S. cerevisiae strains; not conserved in closely related Saccharomyces species
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:7768807]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11956747]
EXISTENCE:direct assay:GO:0031505 fungal-type cell wall organization [PMID:7768807]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7768807|PMID:31537451]
Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18756268]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9335273|PMID:15781460]
EXISTENCE:direct assay:GO:0071597 cellular birth scar [PMID:16672383]
EXISTENCE:genetic interaction:GO:0032120 ascospore-type prospore membrane formation [PMID:18756268]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7768807]
Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to beta-1,3- and beta-1,6-glucan heteropolymer through phosphodiester bond; required for propionic acid resistance; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:17515604]
EXISTENCE:direct assay:GO:0000384 first spliceosomal transesterification activity [PMID:17515604]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:17515604]
Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ('nineteen complex') and acts after Prp2p to promote the first catalytic reaction of splicing
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:20554061|PMID:24868093]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:20554061]
EXISTENCE:direct assay:GO:0017171 serine hydrolase activity [PMID:14645503]
EXISTENCE:mutant phenotype:GO:0006641 triglyceride metabolic process [PMID:20554061]
EXISTENCE:mutant phenotype:GO:0047372 acylglycerol lipase activity [PMID:20554061]
Monoglyceride lipase (MGL) that hydrolyzes fatty acid ethyl esters; required for removal of toxic metabolites in ethanol producing yeast cells; localizes to lipid particles and membranes; member of the eukaryotic serine hydrolase family; functional ortholog of mammalian MGL
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8208248]
Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; MBR1 has a paralog, ISF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10444589]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:8371782]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10444589|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:2065354|PMID:22558355]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:2065354]
EXISTENCE:mutant phenotype:GO:0007163 establishment or maintenance of cell polarity [PMID:23783029]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:22558355]
EXISTENCE:mutant phenotype:GO:0036267 invasive filamentous growth [PMID:23783029]
GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains
phosphatidylinositol/phosphatidylcholine-binding protein
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12869188]
EXISTENCE:direct assay:GO:0031210 phosphatidylcholine binding [PMID:18243114]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:18243114]
Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:31219035]
EXISTENCE:direct assay:GO:0006379 mRNA cleavage [PMID:31219035]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12628920|PMID:12787503]
EXISTENCE:direct assay:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:31219035]
Ubiquitin-binding endonuclease involved in mRNA decay; recruited to stalled ribosomes to promote breakdown of transcript body of nuclear-transcribed mRNA with stalls in translation elongation; cleaves mRNA within A site of colliding ribosome; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:9407032]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:9407032|PMID:18701705]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:14581449]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:22561346]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:8408221]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8408221]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:18701705]
Centromeric CDEIII region binding protein; nucleates kinetochore assembly; required for the structural integrity of elongating spindles; copurifies with subunits of the MIND complex and centromeric nucleosome components (Cse4p and histones H2A, H2B, and H4); phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3; localizes to the kinetochore
phosphopantothenoylcysteine decarboxylase complex subunit CAB3
EXISTENCE:direct assay:GO:0004633 phosphopantothenoylcysteine decarboxylase activity [PMID:19915539]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591|PMID:14562095]
EXISTENCE:direct assay:GO:0071513 phosphopantothenoylcysteine decarboxylase complex [PMID:19915539]
EXISTENCE:direct assay:GO:1990143 CoA-synthesizing protein complex [PMID:23789928]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19266201|PMID:19915539]
EXISTENCE:mutant phenotype:GO:0004633 phosphopantothenoylcysteine decarboxylase activity [PMID:19915539]
Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:7559417]
EXISTENCE:mutant phenotype:GO:0004408 holocytochrome-c synthase activity [PMID:1499554]
EXISTENCE:mutant phenotype:GO:0018063 cytochrome c-heme linkage [PMID:1499554]
Cytochrome c1 heme lyase; involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1; human homolog HCCS can complement yeast cyt2 null mutant
EXISTENCE:direct assay:GO:0032542 sulfiredoxin activity [PMID:14586471]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:14586471]
Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:9774331]
EXISTENCE:direct assay:GO:0030060 L-malate dehydrogenase activity [PMID:1898005]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:10777592]
EXISTENCE:mutant phenotype:GO:0006099 tricarboxylic acid cycle [PMID:3312168]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:3312168]
EXISTENCE:mutant phenotype:GO:0030060 L-malate dehydrogenase activity [PMID:10777592]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated; mutation in human homolog MDH2 causes early-onset severe encephalopathy
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:15294910]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:18787558]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:15294910]
Zinc-binding mitochondrial intermembrane space (IMS) protein; involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11489916|PMID:17804645]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:17272295|PMID:17804645]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:17272295]
Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
translation elongation factor EF1B gamma
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:3528160]
EXISTENCE:direct assay:GO:0006414 translational elongation [PMID:3528160]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23451152|PMID:26777405]
Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:8674114]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position G2815
H(+)-transporting V1 sector ATPase subunit C
Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8188749]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9472039|PMID:8188749]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:8188749]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:8188749|PMID:19053807]
EXISTENCE:genetic interaction:GO:0006904 vesicle docking involved in exocytosis [PMID:27307583]
EXISTENCE:mutant phenotype:GO:0003774 cytoskeletal motor activity [PMID:9472039]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27307583]
Kinesin-like myosin passenger-protein; interacts with Myo2p and enhances its interaction with Sec4p during transport of secretory vesicles; controls actin cable structure and dynamics
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:10982841]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10982841]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:10982841]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16449634]
Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:28727280]
EXISTENCE:mutant phenotype:GO:0006605 protein targeting [PMID:28727280]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:28727280]
Protein that promotes the transport and maturation of Pma1 with Exp1; cleaved specifically by Kex2p; localizes to the Golgi apparatus and to the endoplasmic reticulum in HTP studies
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:35577788]
EXISTENCE:mutant phenotype:GO:0009081 branched-chain amino acid metabolic process [PMID:35577788]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:16121259]
hypothetical protein; regulates actin cable stability and branched-chain amino acid (BCAA) metabolism; involved in mitochondrial quality control and longevity; proposed to be involved in resistance to streptozotocin and camptothecin; localizes to punctate cytosolic structures
EXISTENCE:direct assay:GO:0000243 commitment complex [PMID:7926772]
EXISTENCE:direct assay:GO:0045131 pre-mRNA branch point binding [PMID:7926772]
EXISTENCE:genetic interaction:GO:0000348 mRNA branch site recognition [PMID:18375978]
EXISTENCE:mutant phenotype:GO:0000243 commitment complex [PMID:7926772]
EXISTENCE:mutant phenotype:GO:0000348 mRNA branch site recognition [PMID:9130720]
Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:14704430]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:19759005]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:8654361|PMID:8887673]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:19759005]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:8654361|PMID:8887673]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:8654361|PMID:8887673]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:10409721|PMID:8654361|PMID:8887673]
Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication
Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:29034432]
EXISTENCE:mutant phenotype:GO:0110095 cellular detoxification of aldehyde [PMID:19854918]
NADH-dependent aldehyde reductase; involved in detoxification of furfural; expression is upregulated in cells treated with the aldehydes furfural and glycolaldehyde, the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
hypothetical protein; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0070191 methionine-R-sulfoxide reductase activity [PMID:19049972]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:19049972]
Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
hypothetical protein; identified by homology to Ashbya gossypii
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:8195299]
EXISTENCE:direct assay:GO:0017056 structural constituent of nuclear pore [PMID:19098896]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:20647373]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:20647373]
EXISTENCE:genetic interaction:GO:0036228 protein localization to nuclear inner membrane [PMID:21659568]
EXISTENCE:genetic interaction:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809]
EXISTENCE:mutant phenotype:GO:2000238 regulation of tRNA export from nucleus [PMID:27932586]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:11104765]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:19098896|PMID:12917401|PMID:17803941|PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication
Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004550 nucleoside diphosphate kinase activity [PMID:12472466]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:12472466]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12472466]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:18983998]
Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
hypothetical protein; SWAT-GFP fusion protein localizes to endoplasmic reticulum (ER) while mCherry fusion localizes to both ER and vacuole
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20378542|PMID:14562095|PMID:26928762]
Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19720860|PMID:19001347|PMID:26928762]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026036]
EXISTENCE:mutant phenotype:GO:0010961 cellular magnesium ion homeostasis [PMID:19720860]
EXISTENCE:mutant phenotype:GO:0015095 magnesium ion transmembrane transporter activity [PMID:19720860]
Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
stress-responsive transcriptional activator MSN4
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:23435728|PMID:27637775]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:8321194|PMID:8641288]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12732613|PMID:9472026]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12732613|PMID:9472026]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:18070923]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:27637775|PMID:8650168]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15922872|PMID:11102521]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27637775]
Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome
EXISTENCE:direct assay:GO:0004332 fructose-bisphosphate aldolase activity [PMID:2647491]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16806052|PMID:16962558|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16806052]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:mutant phenotype:GO:0004332 fructose-bisphosphate aldolase activity [PMID:2647491]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:6384192]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:6384192]
Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo
cleavage polyadenylation factor subunit MPE1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:35584695]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:direct assay:GO:0006369 termination of RNA polymerase II transcription [PMID:35584695]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11713271|PMID:35584695]
EXISTENCE:direct assay:GO:0036002 pre-mRNA binding [PMID:25135474|PMID:35584695]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:35584695]
EXISTENCE:genetic interaction:GO:0016567 protein ubiquitination [PMID:25135474]
EXISTENCE:genetic interaction:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11713271]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:32009536]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:11713271]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:25135474]
EXISTENCE:mutant phenotype:GO:0036002 pre-mRNA binding [PMID:25135474]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:32009536]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11713271]
Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase; detects polyadenylation signal in nascent pre-mRNA and triggers 3' end formation of mRNA via cleavage and polyadenylation; binds pre-mRNA via zinc knuckle and RING finger; E3 ubiquitin ligase involved in polyubiquitination of endonuclease Ysh1p; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and a RING finger domain; relocalizes to cytosol in response to hypoxia; homolog of human RBBP6
ARS1109; Autonomously Replicating Sequence
transcription initiation factor IIA subunit gamma
EXISTENCE:direct assay:GO:0005672 transcription factor TFIIA complex [PMID:7862117]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:7862117]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:7604282]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:15132995]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:15132995]
TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa1p, for ribosomal protein gene transcription in vivo; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:11823431]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:15725626]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:22110603]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16418532|PMID:17538013]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:8557736|PMID:8557737|PMID:8565072]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:8557736]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:22331846]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22110603]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:22331846|PMID:8557736|PMID:8557737|PMID:8565072|PMID :9725905]
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16806052]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:16702403]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:31936125]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:16806052]
Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress; homolog of human TPT1
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH)
EXISTENCE:direct assay:GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity [PMID:19571038]
EXISTENCE:mutant phenotype:GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity [PMID:9388293]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9388293]
EXISTENCE:mutant phenotype:GO:0006631 fatty acid metabolic process [PMID:9388293]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9388293]
Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant
DNA damage-responsive RNA polymerase-degradation factor DEF1
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11859374]
EXISTENCE:direct assay:GO:0062058 transcription factor TFIIH holo complex binding [PMID:29180430]
EXISTENCE:genetic interaction:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17537816]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:15863512]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:11859374]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11859374]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:11859374]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:24465179]
EXISTENCE:mutant phenotype:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:29180430]
RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; interacts with general transcription factor TFIIH; mutant is deficient in Zip1p loading onto chromosomes during meiosis; null mutant is prone to self-diploidization; null mutation can be functionally complemented by human UBAP2 and UBAP2
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:17095012]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:27354379]
EXISTENCE:direct assay:GO:0045332 phospholipid translocation [PMID:26235513]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266]
EXISTENCE:mutant phenotype:GO:0033108 mitochondrial respiratory chain complex assembly [PMID:19703468]
Mitochondrial intermembrane space protein; forms complex with Ups2p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12015967]
EXISTENCE:genetic interaction:GO:0007033 vacuole organization [PMID:12015967]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:12015967]
EXISTENCE:genetic interaction:GO:0048017 inositol lipid-mediated signaling [PMID:12015967]
EXISTENCE:genetic interaction:GO:0061093 negative regulation of phospholipid translocation [PMID:29540528]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:12015967]
EXISTENCE:mutant phenotype:GO:0061093 negative regulation of phospholipid translocation [PMID:29540528]
Protein that regulates phospholipid asymmetry at plasma membrane; may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane
Peroxisomal matrix-localized lipase; target of SCFCdc4 ubiquitin ligase complex; peroxisomal, nuclear and cytosolic localization is dependent on the presence of oleate; transcription is regulated by Azf1p; LPX2 has a paralog, EIS1, that arose from the whole genome duplication
centromeric DNA-binding histone H3-like protein CSE4
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:16207811|PMID:23623551]
EXISTENCE:direct assay:GO:0005729 2-micrometer circle DNA [PMID:16966420]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:11063678|PMID:23623551]
EXISTENCE:direct assay:GO:0043505 CENP-A containing nucleosome [PMID:11063678|PMID:19782029]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:16966420]
EXISTENCE:genetic interaction:GO:0000779 condensed chromosome, centromeric region [PMID:11063678]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:7698647]
EXISTENCE:mutant phenotype:GO:0030543 2-micrometer plasmid partitioning [PMID:16966420]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:16207811]
EXISTENCE:mutant phenotype:GO:0061644 protein localization to CENP-A containing chromatin [PMID:28980861]
Centromeric histone H3-like protein; associates with promoters, accessible chromatin, and RNAPII-bound regions; Cse4p associates with centromeres, promoting kinetochore assembly and Sgo1p association with centromeric chromatin; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting bi-orientation; phosphorylation by Cdc7p maintains Cse4p at kinetochores and promotes faithful chromosome segregation; regulated by multiple E3 ligases; complements human homolog CENPA mutations
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:27053666]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:12847291|PMID:16201971|PMID:9180279]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000023 maltose metabolic process [PMID:34140941]
EXISTENCE:mutant phenotype:GO:0000144 cellular bud neck septin ring [PMID:27053666]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:10725226]
EXISTENCE:mutant phenotype:GO:0000902 cell morphogenesis [PMID:16607009|PMID:9427410]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:10725226]
EXISTENCE:mutant phenotype:GO:0007117 budding cell bud growth [PMID:10234786]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9891070]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:15340085]
Serine/threonine protein kinase; regulates the orientation checkpoint, the morphogenesis checkpoint and the metabolic switch from fermentative to oxidative metabolism by phosphorylating the activation loop of Kin4p, Hsl1p and Snf1p respectively; cooperates with Hsl7p in recruiting Hsl1p to the septin ring, a prerequisite for subsequent recruitment, phosphorylation, and degradation of Swe1p; forms part of the bud neck ring; regulates cytokinesis
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
hypothetical protein; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated
putative mannan endo-1,6-alpha-mannosidase
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12421307]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0009272 fungal-type cell wall biogenesis [PMID:12421307]
EXISTENCE:genetic interaction:GO:0007117 budding cell bud growth [PMID:15470258]
Putative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
EXISTENCE:curator inference:GO:0006270 DNA replication initiation [PMID:3888995]
EXISTENCE:curator inference:GO:0006273 lagging strand elongation [PMID:3888995]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:20404922]
EXISTENCE:direct assay:GO:0003896 DNA primase activity [PMID:3061469|PMID:3888995]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15282802]
EXISTENCE:direct assay:GO:0005658 alpha DNA polymerase:primase complex [PMID:3888995|PMID:22593576]
EXISTENCE:direct assay:GO:0006269 DNA replication, synthesis of RNA primer [PMID:2644256]
EXISTENCE:genetic interaction:GO:0003896 DNA primase activity [PMID:8436268]
EXISTENCE:mutant phenotype:GO:0003896 DNA primase activity [PMID:8436268]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:2023935]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:10025407]
Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
hypothetical protein; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the mitochondria; mRNA identified as translated by ribosome profiling data; MMO1 is a non-essential gene
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8114741]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:16449570]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8114741]
EXISTENCE:mutant phenotype:GO:2000222 positive regulation of pseudohyphal growth [PMID:8114741]
Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005821 intermediate layer of spindle pole body [PMID:9215630]
EXISTENCE:direct assay:GO:0005823 central plaque of spindle pole body [PMID:9215630]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:9215630]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:9153752]
Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
EXISTENCE:direct assay:GO:0000815 ESCRT III complex [PMID:12194857]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9606181]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:9606181]
EXISTENCE:mutant phenotype:GO:0070676 intralumenal vesicle formation [PMID:19234443]
One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10468587]
EXISTENCE:genetic interaction:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532773]
EXISTENCE:mutant phenotype:GO:0006880 intracellular sequestering of iron ion [PMID:10588895]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:10468587|PMID:10588895]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:12065597]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532773]
EXISTENCE:physical interaction:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532773]
Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress; acts along with Bol3 at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to client proteins; Fe-S loaded homodimer at steady state; similar to NifU, a bacterial protein required for Fe/S cluster maturation; ortholog of the human NFU1, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS1)
hypothetical protein; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position C2948
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10087931]
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12861007]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12861007|PMID:8887670|PMID:12239282]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8887670]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12861007]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12861007]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8887670]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8887670]
EXISTENCE:genetic interaction:GO:0006006 glucose metabolic process [PMID:8013905]
Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
hypothetical protein; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
UTP glucose-1-phosphate uridylyltransferase
EXISTENCE:direct assay:GO:0070569 uridylyltransferase activity [PMID:22580055]
EXISTENCE:mutant phenotype:GO:0003983 UTP:glucose-1-phosphate uridylyltransferase activity [PMID:7588797]
EXISTENCE:mutant phenotype:GO:0005978 glycogen biosynthetic process [PMID:9252577]
EXISTENCE:mutant phenotype:GO:0006011 UDP-glucose metabolic process [PMID:7588797|PMID:9252577]
EXISTENCE:mutant phenotype:GO:0006078 (1->6)-beta-D-glucan biosynthetic process [PMID:17531808]
UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; involved in PKA-mediated oxidative stress resistance and long-term survival in stationary phase; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:11788821]
EXISTENCE:direct assay:GO:0044695 Dsc E3 ubiquitin ligase complex [PMID:22681890]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:11788821]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:11788821]
EXISTENCE:mutant phenotype:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:11788821]
Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
EXISTENCE:direct assay:GO:0008253 5'-nucleotidase activity [PMID:26071590]
EXISTENCE:direct assay:GO:1990738 pseudouridine 5'-phosphatase activity [PMID:26071590]
hypothetical protein; similar to uncharacterized proteins from other fungi
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
DNA-binding transcription repressor IXR1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:19807692]
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:29449612]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:8634251]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:11115121|PMID:19807692]
EXISTENCE:genetic interaction:GO:0071456 cellular response to hypoxia [PMID:19807692]
EXISTENCE:genetic interaction:GO:2000819 regulation of nucleotide-excision repair [PMID:8538651]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11115121]
EXISTENCE:mutant phenotype:GO:0071456 cellular response to hypoxia [PMID:19807692]
EXISTENCE:mutant phenotype:GO:0090069 regulation of ribosome biogenesis [PMID:29449612]
Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication
malate dehydrogenase (oxaloacetate-decarboxylating)
EXISTENCE:direct assay:GO:0004470 malic enzyme activity [PMID:9603875]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9603875|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0006090 pyruvate metabolic process [PMID:9603875]
EXISTENCE:mutant phenotype:GO:0006520 amino acid metabolic process [PMID:9603875]
Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:8702741]
EXISTENCE:direct assay:GO:0001097 TFIIH-class transcription factor complex binding [PMID:8702741]
EXISTENCE:direct assay:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:10973956]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:9271406]
EXISTENCE:direct assay:GO:0005673 transcription factor TFIIE complex [PMID:1429681|PMID:7961670]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:1331084|PMID:1429681|PMID:7961670]
EXISTENCE:direct assay:GO:0097550 transcription preinitiation complex [PMID:22751016]
EXISTENCE:genetic interaction:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:10973956]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:9188494|PMID:9271406]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:11779853]
TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
tRNA threonylcarbamoyladenosine dehydratase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0061503 tRNA threonylcarbamoyladenosine dehydratase [PMID:23242255]
EXISTENCE:mutant phenotype:GO:0061504 cyclic threonylcarbamoyladenosine biosynthetic process [PMID:23242255]
tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication
ARS1112; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:22659048]
EXISTENCE:direct assay:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
EXISTENCE:mutant phenotype:GO:0004602 glutathione peroxidase activity [PMID:10480913]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:22659048]
EXISTENCE:mutant phenotype:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; glutathione peroxidase activity is neuroprotective in models of Huntington's disease; GPX1 has a paralog, HYR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:8816488]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:24872509]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:11953437]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:24872509]
EXISTENCE:direct assay:GO:0031251 PAN complex [PMID:8816488]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:11953437]
EXISTENCE:mutant phenotype:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:8816488]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:24872509]
EXISTENCE:mutant phenotype:GO:0008143 poly(A) binding [PMID:24872509]
EXISTENCE:physical interaction:GO:0006301 postreplication repair [PMID:11953437]
Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes
EXISTENCE:direct assay:GO:0004017 adenylate kinase activity [PMID:1333436]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8391780]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8391780]
EXISTENCE:direct assay:GO:0006139 nucleobase-containing compound metabolic process [PMID:2549068]
EXISTENCE:direct assay:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:8391780]
EXISTENCE:direct assay:GO:0009041 uridylate kinase activity [PMID:1655742|PMID:2549068]
EXISTENCE:genetic interaction:GO:0004017 adenylate kinase activity [PMID:1333436]
EXISTENCE:mutant phenotype:GO:0009041 uridylate kinase activity [PMID:1655742]
Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:31304628]
SKI complex-associated protein; involved in 3'-5' degradation of long 3'UTR-containing mRNA, poorly translated mRNA, and other RNA regions devoid of ribosomes; proposed to assist the cytoplasmic exosome in a Ska1-SKI complex when a direct interaction between the ribosome and the SKI complex is absent
anaphase promoting complex subunit CDC16
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:direct assay:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:8895471]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0032297 negative regulation of DNA-templated DNA replication initiation [PMID:9660930]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29883473]
Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0016020 membrane [PMID:2826479]
EXISTENCE:genetic interaction:GO:0000027 ribosomal large subunit assembly [PMID:17308036]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:17308036]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17308036]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17308036]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:7739558]
EXISTENCE:physical interaction:GO:0000027 ribosomal large subunit assembly [PMID:17308036]
Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11007476]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11007476]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11063674]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9927444]
EXISTENCE:genetic interaction:GO:0070417 cellular response to cold [PMID:11855848]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11557770]
ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
bifunctional protein farnesyltransferase/protein geranylgeranyltransferase
EXISTENCE:direct assay:GO:0004660 protein farnesyltransferase activity [PMID:1763050]
EXISTENCE:direct assay:GO:0004661 protein geranylgeranyltransferase activity [PMID:2034682]
EXISTENCE:direct assay:GO:0004662 CAAX-protein geranylgeranyltransferase activity [PMID:1400380]
EXISTENCE:direct assay:GO:0005953 CAAX-protein geranylgeranyltransferase complex [PMID:1400380]
EXISTENCE:direct assay:GO:0005965 protein farnesyltransferase complex [PMID:1763050]
EXISTENCE:direct assay:GO:0007323 peptide pheromone maturation [PMID:1763050]
EXISTENCE:direct assay:GO:0018343 protein farnesylation [PMID:1763050]
EXISTENCE:direct assay:GO:0018344 protein geranylgeranylation [PMID:1400380]
Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor
Putative mitochondrial hypothetical protein; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:direct assay:GO:0051568 histone H3-K4 methylation [PMID:11742990|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:15146080]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15146080]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:15146080]
EXISTENCE:mutant phenotype:GO:0034729 histone H3-K79 methylation [PMID:18083099]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Subunit of the COMPASS (Set1C) histone H3K4 methyltransferase complex; required for Set1C stability and optimal activity; COMPASS methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
EXISTENCE:direct assay:GO:0033203 DNA helicase A complex [PMID:9341218]
EXISTENCE:direct assay:GO:0043139 5'-3' DNA helicase activity [PMID:9341218|PMID:8257676]
EXISTENCE:direct assay:GO:0043601 nuclear replisome [PMID:8257676]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:20463880]
EXISTENCE:mutant phenotype:GO:0006301 postreplication repair [PMID:20463880]
Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
EXISTENCE:direct assay:GO:0000274 mitochondrial proton-transporting ATP synthase, stator stalk [PMID:18937496]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0000274 mitochondrial proton-transporting ATP synthase, stator stalk [PMID:1832157]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:1832157]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:1832157]
Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:1986247]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:1986247|PMID:8846888]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:22579222]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8846888]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:2689862|PMID:8846888]
EXISTENCE:mutant phenotype:GO:2001158 positive regulation of proline catabolic process to glutamate [PMID:2689862]
Transcriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15208443|PMID:14562095|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15208443]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15208443]
Protein required for the normal accumulation of 25S and 5.8S rRNAs; nucleolar protein; associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
ARS1113; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407|PMID:9210376]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:10377407]
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; mutation is functionally complemented by human ARPC4
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:8622699]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:8622699]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:15020406]
U1 snRNP protein involved in splicing; interacts with the branchpoint-binding protein during the formation of the second commitment complex
EXISTENCE:direct assay:GO:0004520 endodeoxyribonuclease activity [PMID:8413191]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8413191]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:12198175]
Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0035519 protein K29-linked ubiquitination [PMID:28165462]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:10089879]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10089879]
EXISTENCE:mutant phenotype:GO:0010994 free ubiquitin chain polymerization [PMID:22497224]
EXISTENCE:mutant phenotype:GO:0051974 negative regulation of telomerase activity [PMID:26365526]
EXISTENCE:physical interaction:GO:1904855 proteasome regulatory particle binding [PMID:10688918|PMID:12447385]
Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; involved in K29-linked ubiquitin-dependent protein catabolism, including ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:19797079]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19797078|PMID:19797079|PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:19797079]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:19797079]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:10471698]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12837249]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10198056]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:direct assay:GO:0046513 ceramide biosynthetic process [PMID:15692566]
EXISTENCE:direct assay:GO:0050291 sphingosine N-acyltransferase activity [PMID:15692566]
EXISTENCE:direct assay:GO:0061576 acyl-CoA ceramide synthase complex [PMID:15692566]
EXISTENCE:mutant phenotype:GO:0046513 ceramide biosynthetic process [PMID:11694577]
EXISTENCE:mutant phenotype:GO:0050291 sphingosine N-acyltransferase activity [PMID:11387200|PMID:11694577]
Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:1315784]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:1315784|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:22842922]
EXISTENCE:direct assay:GO:0008290 F-actin capping protein complex [PMID:14769858]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:14769858]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0051016 barbed-end actin filament capping [PMID:14769858|PMID:1315784]
EXISTENCE:mutant phenotype:GO:0008290 F-actin capping protein complex [PMID:14769858]
EXISTENCE:mutant phenotype:GO:0051015 actin filament binding [PMID:14769858]
Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:7596416]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11959998]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:10591633|PMID:7596416]
Intra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:16484372]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:16484372]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:16484372]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:16484372]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position A436
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:29788267]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:29788267]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:29788267]
Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:14704159]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:24003114]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:17142463]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:14704159]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:14704159]
Negative regulator of transcription elongation; contains a TFIIS-like domain that associates with chromatin and a PHD domain that interacts with H3K4me3; multicopy suppressor of temperature-sensitive ess1 mutations, binds RNA polymerase II large subunit
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:10888667]
EXISTENCE:direct assay:GO:0006673 inositol phosphoceramide metabolic process [PMID:19726565]
EXISTENCE:direct assay:GO:0045140 inositol phosphoceramide synthase activity [PMID:19047657]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9092515]
EXISTENCE:mutant phenotype:GO:0045140 inositol phosphoceramide synthase activity [PMID:9092515]
EXISTENCE:physical interaction:GO:0070916 inositol phosphoceramide synthase complex [PMID:19726565]
Phosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase
mitochondrial 37S ribosomal protein YmS16
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
CHM2; GRD7; REN1; VPL2; VPS14; VPS2; VPT14
EXISTENCE:curator inference:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15086794]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12194857]
EXISTENCE:direct assay:GO:0045324 late endosome to vacuole transport [PMID:12194857]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
EXISTENCE:direct assay:GO:0004020 adenylylsulfate kinase activity [PMID:3019265]
EXISTENCE:mutant phenotype:GO:0000096 sulfur amino acid metabolic process [PMID:1101032]
EXISTENCE:mutant phenotype:GO:0004020 adenylylsulfate kinase activity [PMID:197388]
EXISTENCE:mutant phenotype:GO:0019379 sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) [PMID:197388]
Adenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15550248]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8299420]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:23385815]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16520372]
EXISTENCE:direct assay:GO:0007031 peroxisome organization [PMID:12707309]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:20841380]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:25772449]
EXISTENCE:direct assay:GO:0060988 lipid tube assembly [PMID:20841380]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:20841380]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8299420]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:20841380|PMID:26478779]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:12707309]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:8299420]
EXISTENCE:mutant phenotype:GO:0016559 peroxisome fission [PMID:18445678]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:15265985]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:25772449|PMID:26478779]
Dynamin-like GTPase required for vacuolar sorting; promotes fission of retrograde transport carriers from endosome; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
EXISTENCE:direct assay:GO:0004652 polynucleotide adenylyltransferase activity [PMID:1840648]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:1840648]
EXISTENCE:genetic interaction:GO:0071050 sno(s)RNA polyadenylation [PMID:18951092]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:8524265]
Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16096648]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:16156791]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:20008566]
EXISTENCE:direct assay:GO:0043325 phosphatidylinositol-3,4-bisphosphate binding [PMID:16156791]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:16156791]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:16156791]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:16156791]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:29487131]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0016125 sterol metabolic process [PMID:16096648]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
EXISTENCE:mutant phenotype:GO:0055092 sterol homeostasis [PMID:16156791]
Oxysterol-binding phosphatidylserine transferase; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication
ARS1114; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
mitochondrial 54S ribosomal protein YmL13
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:2060626]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2060626]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:2060626]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15885099|PMID:15989961|PMID:16301533|PMID:19748353|P MID:14562095]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:15885099]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:15989961]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:26206314]
EXISTENCE:physical interaction:GO:0071986 Ragulator complex [PMID:15989961]
Subunit of EGO/GSE complex; vacuolar/endosomal membrane-associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from endosome to plasma membrane; targeted to vacuole via AP-3 pathway; loss results in defect in vacuolar acidification
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:12183366|PMID:10619019]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:20126658]
Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
bifunctional hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase FOX2
EXISTENCE:direct assay:GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity [PMID:1551874]
EXISTENCE:direct assay:GO:0004300 enoyl-CoA hydratase activity [PMID:1551874]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:30131444]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:10497229|PMID:1551874]
3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase; multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; mutation is functionally complemented by human HSD17B4
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:18595708]
EXISTENCE:direct assay:GO:0019211 phosphatase activator activity [PMID:18595708]
EXISTENCE:genetic interaction:GO:0007000 nucleolus organization [PMID:18595708]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:17043313]
EXISTENCE:mutant phenotype:GO:0007000 nucleolus organization [PMID:18595708]
EXISTENCE:mutant phenotype:GO:0034503 protein localization to nucleolar rDNA repeats [PMID:18595708|PMID:19362534]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:18923139]
Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
ARS1115; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11935221]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:23027975]
EXISTENCE:genetic interaction:GO:0015908 fatty acid transport [PMID:28365570]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:23027975]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:23027975]
Sterol binding protein involved in the export of acetylated sterols; role in sterol export is redundant with that of PRY1; redundant role with PRY1 and PRY3 in the export of free fatty acids; may be involved in detoxification of hydrophobic compounds; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins)
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:22748138|PMID:23612966]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:22748138]
EXISTENCE:genetic interaction:GO:0036010 protein localization to endosome [PMID:23264632]
EXISTENCE:genetic interaction:GO:0036258 multivesicular body assembly [PMID:22748138]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0005770 late endosome [PMID:11872141]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8163546]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:22748138]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8163546]
Endosomal Rab family GTPase; required for vacuolar protein sorting, endocytosis and multivesicular body (MVB) biogenesis and sorting; required for localization of the CORVET complex to endosomes; involved in autophagy and ionic stress tolerance; similar to Vps21p and Ypt53p; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:19528297]
EXISTENCE:direct assay:GO:0030061 mitochondrial crista [PMID:19528297]
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:19528297|PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:21944719]
EXISTENCE:physical interaction:GO:0045041 protein import into mitochondrial intermembrane space [PMID:21944719]
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic60p is also involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; ortholog of mammalian mitofilin
EXISTENCE:direct assay:GO:0016574 histone ubiquitination [PMID:22570702]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:22570702]
EXISTENCE:mutant phenotype:GO:0036205 histone catabolic process [PMID:22570702]
RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:18298591]
EXISTENCE:genetic interaction:GO:0006914 autophagy [PMID:18298591]
EXISTENCE:genetic interaction:GO:0009267 cellular response to starvation [PMID:18298591]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:15265867]
EXISTENCE:genetic interaction:GO:0048017 inositol lipid-mediated signaling [PMID:15265867]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:10082585]
EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12377769]
EXISTENCE:direct assay:GO:0007118 budding cell apical bud growth [PMID:11957109]
EXISTENCE:direct assay:GO:0042147 retrograde transport, endosome to Golgi [PMID:12686613]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:12446664]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:12446664]
EXISTENCE:mutant phenotype:GO:0090156 cellular sphingolipid homeostasis [PMID:26357016]
EXISTENCE:physical interaction:GO:0000938 GARP complex [PMID:12377769|PMID:12446664]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12377769]
Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:20739461]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:20739461]
EXISTENCE:genetic interaction:GO:2000397 positive regulation of ubiquitin-dependent endocytosis [PMID:20956561]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:23824189]
EXISTENCE:mutant phenotype:GO:0032386 regulation of intracellular transport [PMID:20739461]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000390 spliceosomal complex disassembly [PMID:16357217|PMID:16880513]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591|PMID:14562095]
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:16880513]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:16357217]
EXISTENCE:mutant phenotype:GO:0000390 spliceosomal complex disassembly [PMID:16880513]
Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:28757607]
Subunit of ribosome-associated quality control trigger complex (RQT); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9582098]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9582098]
EXISTENCE:mutant phenotype:GO:0000464 endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:34686661]
Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions
DNA-directed RNA polymerase III subunit C37
translation initiation factor eIF2B subunit alpha
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8506384]
EXISTENCE:direct assay:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8506384|PMID:8336705]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:8506384]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8506384]
EXISTENCE:genetic interaction:GO:0006446 regulation of translational initiation [PMID:8887689]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:8887689]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:8099443|PMID:34223816]
EXISTENCE:mutant phenotype:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8099443]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:8099443|PMID:9032257]
EXISTENCE:mutant phenotype:GO:0030234 enzyme regulator activity [PMID:8099443|PMID:8887689|PMID:9032257]
EXISTENCE:mutant phenotype:GO:0045948 positive regulation of translational initiation [PMID:34223816]
EXISTENCE:mutant phenotype:GO:1900036 positive regulation of cellular response to heat [PMID:34223816]
EXISTENCE:mutant phenotype:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:physical interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8506384]
EXISTENCE:physical interaction:GO:0006446 regulation of translational initiation [PMID:8887689]
Alpha subunit of translation initiation factor eIF2B (eIF2Balpha); guanine-nucleotide exchange factor for eIF2; mutations in eIF2Balpha alter localization of eIF2B; activity regulated by phosphorylated eIF2; positive regulator of GCN4 expression; mutations in human ortholog EIF2B2 cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); human ortholog EIF2B1 can complement yeast null mutant
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:direct assay:GO:0030140 trans-Golgi network transport vesicle [PMID:16498409]
EXISTENCE:direct assay:GO:0034044 exomer complex [PMID:17000877]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:physical interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:16498409]
Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8649382]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:15367655]
Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:19379692]
EXISTENCE:mutant phenotype:GO:0006479 protein methylation [PMID:31960028]
EXISTENCE:mutant phenotype:GO:0045835 negative regulation of meiotic nuclear division [PMID:11711434]
Defining member of the SET3 histone deacetylase complex; which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12808035]
EXISTENCE:physical interaction:GO:0016192 vesicle-mediated transport [PMID:12808035]
Golgi membrane hypothetical protein; interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting; conserved from yeast to human
EXISTENCE:direct assay:GO:0004630 phospholipase D activity [PMID:11514437]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:9693364]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21954159]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:10848624]
EXISTENCE:direct assay:GO:0006644 phospholipid metabolic process [PMID:8576189]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:genetic interaction:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:18036176]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:12702348]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:8576189]
EXISTENCE:mutant phenotype:GO:0031321 ascospore-type prospore assembly [PMID:16554438]
Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with 'foot domain' of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:8045902]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11443099]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:8376332]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:7899075|PMID:8045902|PMID:8416910]
EXISTENCE:mutant phenotype:GO:0090295 nitrogen catabolite repression of transcription [PMID:1944286|PMID:6757722|PMID:7899075]
Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11029042]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11029042]
EXISTENCE:direct assay:GO:1904902 ESCRT III complex assembly [PMID:31273031]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:11029042]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17130288]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:12194857]
Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16009724|PMID:14576278|PMID:16823961|PMID:26928762]
EXISTENCE:direct assay:GO:0030014 CCR4-NOT complex [PMID:11113136]
EXISTENCE:genetic interaction:GO:0016559 peroxisome fission [PMID:18445678]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:16009724]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11113136]
EXISTENCE:physical interaction:GO:0000266 mitochondrial fission [PMID:16009724]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11113136]
WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
tRNA N6-adenosine threonylcarbamoyltransferase
EXISTENCE:direct assay:GO:0000408 EKC/KEOPS complex [PMID:16564010|PMID:16874308]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16874308]
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:23620299]
EXISTENCE:direct assay:GO:0031490 chromatin DNA binding [PMID:16874308]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18439903]
EXISTENCE:mutant phenotype:GO:0070525 tRNA threonylcarbamoyladenosine metabolic process [PMID:21459853|PMID:21183954]
EXISTENCE:physical interaction:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16874308]
ADP/GDP nucleotidase, essential functional component of KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; homolog of human OSGEP
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:15039776]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15039776]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9199164|PMID:15623581|PMID:15707981]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:12499351]
EXISTENCE:genetic interaction:GO:0015193 L-proline transmembrane transporter activity [PMID:14968425]
EXISTENCE:mutant phenotype:GO:0001761 beta-alanine transmembrane transporter activity [PMID:29570051]
EXISTENCE:mutant phenotype:GO:0005771 multivesicular body [PMID:14523026]
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:6759873]
EXISTENCE:mutant phenotype:GO:0015171 amino acid transmembrane transporter activity [PMID:6759873]
EXISTENCE:mutant phenotype:GO:0015203 polyamine transmembrane transporter activity [PMID:15707981]
EXISTENCE:mutant phenotype:GO:0015846 polyamine transport [PMID:15707981]
General amino acid permease; involved in uptake of all L-amino acids, some D-amino acids, related compounds, toxic analogs and polyamines; regulated by nitrogen source; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; essential for invasive growth
ARS1116; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21777356|PMID:14562095]
hypothetical protein; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:23910823]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:15247238]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:20070376]
Mitochondrial inner membrane protein; role in mitophagy is disputed; implicated in cell wall biogenesis, the oxidative stress response, life span during starvation, and cell death; SUN family member; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0046390 ribose phosphate biosynthetic process [PMID:21663798]
EXISTENCE:direct assay:GO:0050278 sedoheptulose-bisphosphatase activity [PMID:21663798]
EXISTENCE:mutant phenotype:GO:0046390 ribose phosphate biosynthetic process [PMID:21663798]
EXISTENCE:mutant phenotype:GO:0050278 sedoheptulose-bisphosphatase activity [PMID:21663798]
Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11056382]
hypothetical protein that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates
hypothetical protein; epitope-tagged protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093]
EXISTENCE:expression pattern:GO:0009060 aerobic respiration [PMID:10618406]
Perilipin, involved in formation and stability of lipid droplets; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7622566|PMID:30341817]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:1400414|PMID:2016313]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:22308335]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:25416945]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:30341817]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:22308335]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:17289584|PMID:21348863]
EXISTENCE:direct assay:GO:0043085 positive regulation of catalytic activity [PMID:22308335]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:31062022]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:31062022]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:12456659]
EXISTENCE:mutant phenotype:GO:0007117 budding cell bud growth [PMID:13680156]
EXISTENCE:mutant phenotype:GO:0031116 positive regulation of microtubule polymerization [PMID:7622567]
EXISTENCE:mutant phenotype:GO:0098841 protein localization to cell division site after cytokinesis [PMID:25416945]
EXISTENCE:physical interaction:GO:0030332 cyclin binding [PMID:7622566]
Histone chaperone and assembly chaperone for Rps6a/b; involved in histone exchange, replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; promotes solubility of Rps6a, and 40S ribosomal subunit biogenesis; role in transport of H2A and H2B histones to the nucleus; regulates microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0051051 negative regulation of transport [PMID:21912684]
Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:14570869]
EXISTENCE:direct assay:GO:0015079 potassium ion transmembrane transporter activity [PMID:2072919]
EXISTENCE:direct assay:GO:0030007 cellular potassium ion homeostasis [PMID:2072919]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:33640956]
EXISTENCE:mutant phenotype:GO:0042391 regulation of membrane potential [PMID:21424545]
Component of the Trk1p-Trk2p potassium transport system; contributes to K(+) supply and maintenance of plasma-membrane potential; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:34088313]
EXISTENCE:mutant phenotype:GO:0044395 protein targeting to vacuolar membrane [PMID:34088313]
Vacuole membrane protein that interacts with Atg8p; functions in the vacuolar membrane recruitment of Atg8p to facilitate the degradation of vacuolar integral membrane proteins during early-stationary vacuole turnover (EVT), a general degradation process for vacuolar integral membrane proteins when cells enter stationary phase; null mutant is defective in stationary phase-induced vacuolar microdomain formation; homolog of S. pombe hfl1; homolog of human TMEM184
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:1703236]
EXISTENCE:direct assay:GO:0048250 iron import into the mitochondrion [PMID:12902335]
EXISTENCE:mutant phenotype:GO:0005381 iron ion transmembrane transporter activity [PMID:12902335]
Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10477278|PMID:10531356]
EXISTENCE:direct assay:GO:0042392 sphingosine-1-phosphate phosphatase activity [PMID:10856228]
EXISTENCE:direct assay:GO:0046839 phospholipid dephosphorylation [PMID:10856228]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9353337]
Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000235 astral microtubule [PMID:16580990]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:25941405]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:16580990]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:12566428|PMID:15965467]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:15642746]
EXISTENCE:direct assay:GO:0008569 minus-end-directed microtubule motor activity [PMID:25941405|PMID:16873064]
EXISTENCE:genetic interaction:GO:0000070 mitotic sister chromatid segregation [PMID:7860634]
EXISTENCE:genetic interaction:GO:0030473 nuclear migration along microtubule [PMID:9201714]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:9281582]
EXISTENCE:mutant phenotype:GO:0008569 minus-end-directed microtubule motor activity [PMID:25941405]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:9201714]
EXISTENCE:mutant phenotype:GO:0040001 establishment of mitotic spindle localization [PMID:21633107]
EXISTENCE:physical interaction:GO:0005868 cytoplasmic dynein complex [PMID:9658168]
Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:18417612]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:10526184]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18417612]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:18417612|PMID:26928762]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:1448099]
EXISTENCE:genetic interaction:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:12810699]
EXISTENCE:physical interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:9184220]
Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi
tRNA (uracil(54)-C(5))-methyltransferase
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:17205207]
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:10864043]
EXISTENCE:direct assay:GO:0030696 tRNA (m5U54) methyltransferase activity [PMID:10864043]
EXISTENCE:direct assay:GO:0051908 double-stranded DNA 5'-3' exodeoxyribonuclease activity [PMID:17205207]
EXISTENCE:mutant phenotype:GO:0030696 tRNA (m5U54) methyltransferase activity [PMID:10864043]
tRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0005978 glycogen biosynthetic process [PMID:8524228]
EXISTENCE:genetic interaction:GO:0008466 glycogenin glucosyltransferase activity [PMID:8524228]
Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole respectively; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:1502180]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:1502180]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:1502180]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:24471916]
EXISTENCE:direct assay:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:30281017|PMID:29192585]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0016281 eukaryotic translation initiation factor 4F complex [PMID:2648398|PMID:10409745]
Translation initiation factor eIF4A; DEAD-box RNA helicase that couples ATPase activity to mRNA binding and unwinding; ATP hydrolysis accelerates the recruitment of mRNA during 48S preinitiation complex (PIC) assembly; ATPase activity stimulated by either eIF4G1 or by the PIC in an eIF3g and eIFGi subunit-dependent manner, independent of eIF4E and eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E and eIF4G; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095|PMID:22588997]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911|PMID:22588997]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:22588997]
EXISTENCE:physical interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:21724601]
Subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit; Utp30p is peripherally associated with 90S preribosome and appears partially redundant with its paralog Cic1p
EXISTENCE:direct assay:GO:0000030 mannosyltransferase activity [PMID:8631921]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8631921]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:8631921]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:8631921]
Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9160748]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2975
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:9405484]
EXISTENCE:physical interaction:GO:0005673 transcription factor TFIIE complex [PMID:9188494]
TFIIE small subunit; involved in RNA polymerase II transcription initiation
EXISTENCE:direct assay:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:26638174]
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:26638174]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22083961|PMID:8582632]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:23175604]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22083961]
EXISTENCE:mutant phenotype:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23175604]
EXISTENCE:mutant phenotype:GO:0000460 maturation of 5.8S rRNA [PMID:22083961]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22083961]
EXISTENCE:physical interaction:GO:0030687 preribosome, large subunit precursor [PMID:23175604]
Endonuclease involved in pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to cleave pre-rRNA at a specific site during rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:17551510]
Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:16107694]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:16107694]
Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004130 cytochrome-c peroxidase activity [PMID:21334283|PMID:17580971]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:11914276|PMID:14576278|PMID:24769239]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:6290489]
EXISTENCE:mutant phenotype:GO:0004130 cytochrome-c peroxidase activity [PMID:10589830|PMID:21334283]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10589830]
Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria; acts as hydrogen peroxide sensor in mitochondria, involved in the response to oxidative stress
bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase GPT2
EXISTENCE:direct assay:GO:0004366 glycerol-3-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:22267742|PMID:9401016]
EXISTENCE:direct assay:GO:0008654 phospholipid biosynthetic process [PMID:11544256]
EXISTENCE:direct assay:GO:0016287 glycerone-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:mutant phenotype:GO:0004366 glycerol-3-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:11544256]
EXISTENCE:mutant phenotype:GO:0090207 regulation of triglyceride metabolic process [PMID:31421179]
Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17110339]
EXISTENCE:direct assay:GO:0033106 cis-Golgi network membrane [PMID:9564032]
EXISTENCE:direct assay:GO:1990070 TRAPPI protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990072 TRAPPIII protein complex [PMID:20375281]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:11102533|PMID:17041589|PMID:8590804]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8590804]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:25581738]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17287728]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11239471|PMID:8590804]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11239471]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:25581738]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:25581738]
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles
uroporphyrinogen-III C-methyltransferase
EXISTENCE:direct assay:GO:0004851 uroporphyrin-III C-methyltransferase activity [PMID:10051442]
EXISTENCE:mutant phenotype:GO:0019354 siroheme biosynthetic process [PMID:9003798]
S-adenosyl-L-methionine uroporphyrinogen III transmethylase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:18625724]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18625724]
EXISTENCE:direct assay:GO:0051537 2 iron, 2 sulfur cluster binding [PMID:27166425]
EXISTENCE:direct assay:GO:0051539 4 iron, 4 sulfur cluster binding [PMID:27166425]
EXISTENCE:mutant phenotype:GO:0009055 electron transfer activity [PMID:20802492]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:20802492|PMID:18625724]
EXISTENCE:mutant phenotype:GO:0045019 negative regulation of nitric oxide biosynthetic process [PMID:27178802]
EXISTENCE:mutant phenotype:GO:1901299 negative regulation of hydrogen peroxide-mediated programmed cell death [PMID:27178802]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27178802]
Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18p in an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress; inviability of the null mutant is functionally complemented by human CIAPIN1
phosphopantothenoylcysteine decarboxylase complex subunit SIS2
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:19915539]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:7705654]
EXISTENCE:direct assay:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:9636153]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7705654]
EXISTENCE:direct assay:GO:0032515 negative regulation of phosphoprotein phosphatase activity [PMID:9636153]
EXISTENCE:genetic interaction:GO:0007346 regulation of mitotic cell cycle [PMID:10022927]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19915539]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:9636153]
Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
hypothetical protein; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
hypothetical protein; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication
ARS1118; Autonomously Replicating Sequence
S-glutathionyl-(chloro)hydroquinone reductase
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:16709151|PMID:20388120|PMID:22686328]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16936141|PMID:14562095]
EXISTENCE:direct assay:GO:0006749 glutathione metabolic process [PMID:20388120|PMID:22686328]
S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR); glutathione transferase involved in cell-surface biosynthesis and architecture; catalyzes glutathione (GSH)-dependent reduction of GS-trichloro-p-hydroquinone to trichloro-p-hydroquinone; expression upregulated upon exposure to genotoxic agents, such as methyl methanesulfonate, cisplatin and bleomycin; not an essential gene; similar to YGR154C; green fluorescent protein (GFP)-fusion protein localizes to cytoplasm
EXISTENCE:physical interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:18160399]
Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:35098628]
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:35098628]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:35098628]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:35098628|PMID:11557775]
EXISTENCE:mutant phenotype:GO:1903432 regulation of TORC1 signaling [PMID:35098628]
Vacuolar SNX-BAR protein, regulates TORC1 signaling during autophagy induction; binds phosphatidylinositol-3-phosphate; vacuolar localization of Vps501p is dependent upon direct interaction with Iml1p and a unique lipid binding specificity that is also required for its function; VPS501 has paralog VPS5 that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24249226|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24249226|PMID:14690591|PMID:24769239]
EXISTENCE:direct assay:GO:0034414 tRNA 3'-trailer cleavage, endonucleolytic [PMID:12711671]
EXISTENCE:direct assay:GO:0042780 tRNA 3'-end processing [PMID:24249226]
EXISTENCE:direct assay:GO:0042781 3'-tRNA processing endoribonuclease activity [PMID:12711671|PMID:24249226]
EXISTENCE:direct assay:GO:1990180 mitochondrial tRNA 3'-end processing [PMID:24249226]
EXISTENCE:mutant phenotype:GO:0034414 tRNA 3'-trailer cleavage, endonucleolytic [PMID:24249226]
EXISTENCE:mutant phenotype:GO:1990180 mitochondrial tRNA 3'-end processing [PMID:24249226]
tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
methylenetetrahydrofolate dehydrogenase (NAD(+))
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8608153]
EXISTENCE:direct assay:GO:0006730 one-carbon metabolic process [PMID:8608153]
EXISTENCE:direct assay:GO:0009113 purine nucleobase biosynthetic process [PMID:8608153]
EXISTENCE:mutant phenotype:GO:0004487 methylenetetrahydrofolate dehydrogenase (NAD+) activity [PMID:8416923]
EXISTENCE:mutant phenotype:GO:0006730 one-carbon metabolic process [PMID:8608153]
EXISTENCE:mutant phenotype:GO:0009113 purine nucleobase biosynthetic process [PMID:8608153]
EXISTENCE:mutant phenotype:GO:0009396 folic acid-containing compound biosynthetic process [PMID:8608153]
NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
rRNA-binding ribosome biosynthesis protein RPF2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11864606]
EXISTENCE:direct assay:GO:0008097 5S rRNA binding [PMID:25855814|PMID:11864606]
EXISTENCE:direct assay:GO:0008312 7S RNA binding [PMID:11864606]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:11864606]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17443350]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0008097 5S rRNA binding [PMID:25855814]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
Essential protein involved in rRNA maturation and ribosomal assembly; involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21818277]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:18046406|PMID:11823431]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:8524308]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:7813444|PMID:21036941]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:22331846|PMID:7813444|PMID:7862658|PMID:9725905]
Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133
ribosome dissociation factor GTPase HBS1
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:20947765]
EXISTENCE:direct assay:GO:0032790 ribosome disassembly [PMID:20947765]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:21623367]
EXISTENCE:direct assay:GO:1990533 Dom34-Hbs1 complex [PMID:21623367]
EXISTENCE:genetic interaction:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:16554824]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:24424461]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19481524]
GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication
mitochondrial 54S ribosomal protein YmL20
EXISTENCE:direct assay:GO:0000378 RNA exon ligation [PMID:23685439]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:1825134]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:1825134]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:16103217|PMID:1825134]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:1825134]
EXISTENCE:mutant phenotype:GO:0016073 snRNA metabolic process [PMID:19458033]
EXISTENCE:mutant phenotype:GO:0040031 snRNA modification [PMID:19458033]
DEAH-box RNA helicase; involved in the second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12963738]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:12963738]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:12963738]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:27325672]
EXISTENCE:mutant phenotype:GO:0035694 mitochondrial protein catabolic process [PMID:27325672]
EXISTENCE:mutant phenotype:GO:0051775 response to redox state [PMID:31044600]
Metalloendopeptidase of the mitochondrial inner membrane; important for adaptive responses to various homeostatic insults, preservation of normal mitochondrial function under damage-eliciting conditions, and stability of respiratory supercomplexes; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; zebrafish ortholog has a role in organ development and mouse ortholog is also required for respiratory supercomplex stability
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:16107716]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17178117]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:18194531]
Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0004771 sterol esterase activity [PMID:20332534]
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:16135509|PMID:20332534]
EXISTENCE:direct assay:GO:0006642 triglyceride mobilization [PMID:16135509]
EXISTENCE:direct assay:GO:0042171 lysophosphatidic acid acyltransferase activity [PMID:20332534]
EXISTENCE:direct assay:GO:0047499 calcium-independent phospholipase A2 activity [PMID:20332534]
EXISTENCE:genetic interaction:GO:0007114 cell budding [PMID:19150427]
EXISTENCE:genetic interaction:GO:0019433 triglyceride catabolic process [PMID:16267052]
EXISTENCE:mutant phenotype:GO:0004771 sterol esterase activity [PMID:20332534]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:16135509|PMID:20332534]
EXISTENCE:mutant phenotype:GO:0006642 triglyceride mobilization [PMID:16135509]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:20332534]
EXISTENCE:mutant phenotype:GO:0007114 cell budding [PMID:19150427]
EXISTENCE:mutant phenotype:GO:0019433 triglyceride catabolic process [PMID:16267052]
EXISTENCE:mutant phenotype:GO:0047499 calcium-independent phospholipase A2 activity [PMID:20332534]
Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:14767053]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23613772|PMID:14562095]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:14767053|PMID:15282802|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14767053|PMID:15282802|PMID:14562095]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:23613772]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:14767053]
EXISTENCE:genetic interaction:GO:0005094 Rho GDP-dissociation inhibitor activity [PMID:15215315]
EXISTENCE:genetic interaction:GO:0035023 regulation of Rho protein signal transduction [PMID:15215315]
EXISTENCE:mutant phenotype:GO:0030011 maintenance of cell polarity [PMID:14767053]
EXISTENCE:physical interaction:GO:0035023 regulation of Rho protein signal transduction [PMID:15215315]
Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:23382076]
EXISTENCE:mutant phenotype:GO:0006139 nucleobase-containing compound metabolic process [PMID:9744871]
Repressor of transcription at Start; binds to SBF (Swi4p-Swi6p) at SCB promoter elements in G1 to repress transcription; inhibitor of Start that retains Cdc28-Cln3 at the ER; acts with Ydj1p to promote release of the complex in a growth-mediated positive feedback loop to trigger Start; Srl3p is unstable and degraded by SCF-Grr1 after Cdc28p-dependent phosphorylation; contains a GTB (G1/S transcription factor binding) motif; binds SBF-regulated promoters in HU-treated cells; Cdc28p substrate
ARS1120; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:8702624]
Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340|PMID:17507646]
EXISTENCE:direct assay:GO:0042937 tripeptide transmembrane transporter activity [PMID:16096264]
EXISTENCE:direct assay:GO:0042938 dipeptide transport [PMID:16096264]
EXISTENCE:direct assay:GO:0042939 tripeptide transport [PMID:16096264]
EXISTENCE:direct assay:GO:0071916 dipeptide transmembrane transporter activity [PMID:16096264]
EXISTENCE:mutant phenotype:GO:0015833 peptide transport [PMID:8264579]
EXISTENCE:mutant phenotype:GO:1904680 peptide transmembrane transporter activity [PMID:8264579]
Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by N-end rule pathway via repression by Cup9p; targeted to vacuole via AP-3 pathway
ubiquitin-ribosomal 60S subunit protein L40B fusion protein
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:22096102]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:2538753]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:22995916]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:22995916]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:8035826]
Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10085285]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:10085285]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:16027220]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:24152732]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:10085285]
EXISTENCE:genetic interaction:GO:0034398 telomere tethering at nuclear periphery [PMID:10638763]
EXISTENCE:genetic interaction:GO:0043021 ribonucleoprotein complex binding [PMID:15692572]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:14718167]
EXISTENCE:genetic interaction:GO:1901925 negative regulation of protein import into nucleus during spindle assembly checkpoint [PMID:23177738]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:21036941]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:14718167]
EXISTENCE:mutant phenotype:GO:0090204 protein localization to nuclear pore [PMID:19933151]
EXISTENCE:mutant phenotype:GO:0140586 promoter-terminator loop anchoring activity [PMID:19933151]
EXISTENCE:physical interaction:GO:0043021 ribonucleoprotein complex binding [PMID:15692572]
Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
chromatin DNA-binding EKC/KEOPS complex subunit PCC1
EXISTENCE:direct assay:GO:0000408 EKC/KEOPS complex [PMID:16874308]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16874308]
EXISTENCE:mutant phenotype:GO:0070525 tRNA threonylcarbamoyladenosine metabolic process [PMID:21183954|PMID:21459853]
Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; other complex members are Kae1p, Gon7p, Bud32p, and Cgi121p; ortholog of human LAGE3
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:22375831]
hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl2p and Esl1p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; ESL2 has a paralog, ESL1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity [PMID:16330239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:196563]
EXISTENCE:expression pattern:GO:0006094 gluconeogenesis [PMID:2689220]
EXISTENCE:mutant phenotype:GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity [PMID:2689220]
Phosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:10527495]
Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:1495962|PMID:2683089]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:1495962]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9705508]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1495962]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3303332]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:1495962]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1495962|PMID:3303332]
Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; controls cellular ATP levels; also involved in regulation of meiotic recombination at specific genes
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15645486]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:15645486]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15645486]
Transmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12897051|PMID:12975325|PMID:12198162]
EXISTENCE:direct assay:GO:0005677 chromatin silencing complex [PMID:8622770]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:3297920]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:2684414]
Protein involved in silencing at mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin
ARS1123; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0000128 flocculation [PMID:19420680|PMID:11027318|PMID:19087208]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:11027318|PMID:19087208]
EXISTENCE:mutant phenotype:GO:0044182 filamentous growth of a population of unicellular organisms [PMID:11027318]
Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23658021]
Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
Putative transporter of the MRP subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23047103]
EXISTENCE:genetic interaction:GO:1903826 L-arginine transmembrane transport [PMID:23047103]
EXISTENCE:mutant phenotype:GO:1903826 L-arginine transmembrane transport [PMID:23047103]
Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:21490148]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21490148]
Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; expressed at a very low level; potential role in resistance to oxidative stress and modulation of the PKA pathway; GEX2 has a paralog, GEX1, that arose from a segmental duplication
TEL11R; Telomeric region on the right arm of Chromosome XI; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats