Saccharomyces cerevisiae S288C chromosome X, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (12-APR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006943.\n\nOn Apr 26, 2011 this sequence version replaced NC_001142.8.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL10L; Telomeric region on the left arm of Chromosome X; composed of an X element core sequence, X element combinatorial repeats, a long Y' element, and a short terminal stretch of telomeric repeats
ARS1001; Autonomously Replicating Sequence
Putative Y' element ATP-dependent helicase
ARS1002; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole; active during alcoholic fermentation; regulated by anaerobiosis, negatively regulated by oxygen; repressed by heme; identical to Pau14p
EXISTENCE:genetic interaction:GO:0005048 signal sequence binding [PMID:8636229]
EXISTENCE:genetic interaction:GO:0006623 protein targeting to vacuole [PMID:8636229]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:8662642]
Putative membrane glycoprotein; has strong similarity to Vth1p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
ARS1003; Autonomously Replicating Sequence
EXISTENCE:genetic interaction:GO:0046352 disaccharide catabolic process [PMID:20562106]
EXISTENCE:mutant phenotype:GO:0004574 oligo-1,6-glucosidase activity [PMID:20562106]
Alpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima4p overexpression allows the ima1 null mutant to grow on isomaltose; identical to IMA3
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005354 galactose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Putative hexose transporter that is nearly identical to Hxt11p; has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31945465]
Mitochondrial protein, putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in OAF1/PIP2-dependent manner; promoter contains oleate response element consensus sequence; mutation decreases salt tolerance; non-essential gene
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
ARS1004; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004574 oligo-1,6-glucosidase activity [PMID:20471265]
EXISTENCE:genetic interaction:GO:0046352 disaccharide catabolic process [PMID:20562106|PMID:20471265]
Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; most distant member of the IMA isomaltase family, but with similar catalytic properties as Ima1p and Ima2p; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose; can cleave alpha-1,3 linkage of nigerose and turanose and alpha-1,5 linkage of leucrose and is very sensitive to temperature in vitro
hypothetical protein with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose
hypothetical protein that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
EXISTENCE:direct assay:GO:0005427 proton-dependent oligopeptide secondary active transmembrane transporter activity [PMID:16149917]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19496824]
EXISTENCE:direct assay:GO:0006857 oligopeptide transport [PMID:16149917]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:10788431]
EXISTENCE:mutant phenotype:GO:0035673 oligopeptide transmembrane transporter activity [PMID:10652283]
Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:24034674]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:12876220]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:19687296|PMID:22471590]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:15536088]
RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:9343381]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2104848|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000958 mitochondrial mRNA catabolic process [PMID:9343381]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9343381]
Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
EXISTENCE:direct assay:GO:0004520 endodeoxyribonuclease activity [PMID:3286639]
EXISTENCE:direct assay:GO:0004529 exodeoxyribonuclease activity [PMID:3286639]
EXISTENCE:direct assay:GO:0004540 ribonuclease activity [PMID:3286639]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17244531]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:17244531|PMID:3286639]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:31266891]
EXISTENCE:direct assay:GO:0006308 DNA catabolic process [PMID:3286639]
EXISTENCE:direct assay:GO:0006401 RNA catabolic process [PMID:3286639]
EXISTENCE:direct assay:GO:0051607 defense response to virus [PMID:31266891]
EXISTENCE:mutant phenotype:GO:0006309 apoptotic DNA fragmentation [PMID:22727375]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:8087883]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17244531]
Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:16687571]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:16687571]
EXISTENCE:mutant phenotype:GO:0035653 clathrin-coated vesicle cargo loading, AP-1-mediated [PMID:30523155]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:16687571]
AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
hypothetical protein; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:genetic interaction:GO:0009306 protein secretion [PMID:8655575]
hypothetical protein; involved in secretion of proteins that lack classical secretory signal sequences; SWAT-GFP and mCherry fusion proteins localize to the cytosol
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15537705]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15537705]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:11287615]
EXISTENCE:mutant phenotype:GO:0000149 SNARE binding [PMID:11287615]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10769031|PMID:11378903]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:24272750]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:15537705]
F-box protein involved in recycling endocytosed proteins; involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth; direct interaction with C-terminal cytoplasmic region of Drs2p plays an important role for Drs2p function in endocytic recycling pathway; required for turnover of histone H3 variant Cse4p
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:8969185]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:8065365]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
Subunit of the SF3a splicing factor complex; required for spliceosome assembly
EXISTENCE:direct assay:GO:0042805 actinin binding [PMID:34010635]
EXISTENCE:mutant phenotype:GO:0051510 regulation of unidimensional cell growth [PMID:34010635]
EXISTENCE:mutant phenotype:GO:0080135 regulation of cellular response to stress [PMID:34010635]
Endocytic site protein required for stress-induced cell elongation; may link the polarization signal with the endocytic machinery to facilitate adaptive morphogenesis under conditions of stress; interacts with the polarity regulator Cdc42p, the SH3 domains of actin patch components and actin filaments; contains a Rho-GAP domain lacking the catalytic arginine finger, PXXP motifs and an xACT (F-actin binding) motif; promoter contains a consensus binding sequence for transcription factor Abf1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0006099 tricarboxylic acid cycle [PMID:30834327]
Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
hypothetical protein; conserved among S. cerevisiae strains, not conserved in closely related Saccharomyces species; protein detected via large-scale protein-protein interaction studies and enrichment-based proteogenomics; YJL199C is not an essential gene
SPX domain-containing inorganic phosphate transporter
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21143198]
EXISTENCE:genetic interaction:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:11779791]
EXISTENCE:genetic interaction:GO:0006817 phosphate ion transport [PMID:11779791]
EXISTENCE:genetic interaction:GO:2000185 regulation of phosphate transmembrane transport [PMID:12821119]
EXISTENCE:mutant phenotype:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:12581367]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:17804816]
Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
putative ubiquitin-specific protease UBP12
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:29309037]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:31665643]
EXISTENCE:genetic interaction:GO:0016579 protein deubiquitination [PMID:31665643]
EXISTENCE:genetic interaction:GO:2001020 regulation of response to DNA damage stimulus [PMID:31665643]
EXISTENCE:mutant phenotype:GO:0010637 negative regulation of mitochondrial fusion [PMID:23317502]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:23317502|PMID:29309037]
Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm
ARS1005; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0009922 fatty acid elongase activity [PMID:12684876]
EXISTENCE:mutant phenotype:GO:0019368 fatty acid elongation, unsaturated fatty acid [PMID:10850979]
Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:9159120|PMID:9288745]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194|PMID:9288745]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8083240]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:8083240]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:8083240]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:8083240]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:10075735]
EXISTENCE:mutant phenotype:GO:0003682 chromatin binding [PMID:10075735]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:10075735]
EXISTENCE:mutant phenotype:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:10075735|PMID:23376927|PMID:8538771|PMID:9335335]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:9990508]
EXISTENCE:mutant phenotype:GO:0140530 MCM complex loading [PMID:29795547]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10075735|PMID:9312054]
Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; dual role as a mitotic cyclin-dependent kinase inhibitor, that binds to and inhibits Cdc28p/Clb2p along with Sic1p, contributing to mitotic exit; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress
hypothetical protein; predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12230471|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12230471]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12230471]
ER-membrane protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5 of TOM (Translocase of the Mitochondrial Outer Membrane) complex; suppressor of pma1-7, deletion of SOP4 slows down export of wild-type Pma1p and Pma1-7 from the ER
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:9858605]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:2277060]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15125836]
Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:8253069]
EXISTENCE:direct assay:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8253069]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004713 protein tyrosine kinase activity [PMID:8253069]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10805747|PMID:15282802]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10805747|PMID:15282802]
EXISTENCE:direct assay:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:8930890]
EXISTENCE:genetic interaction:GO:0000320 re-entry into mitotic cell cycle [PMID:15107621]
EXISTENCE:genetic interaction:GO:0010697 negative regulation of mitotic spindle pole body separation [PMID:8887667]
EXISTENCE:genetic interaction:GO:0090154 positive regulation of sphingolipid biosynthetic process [PMID:26634277]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8253069]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0010697 negative regulation of mitotic spindle pole body separation [PMID:8887667]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:10619027]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:9744879]
EXISTENCE:mutant phenotype:GO:0090154 positive regulation of sphingolipid biosynthetic process [PMID:26634277]
Protein kinase that regulates the G2/M transition; negative regulator of the Cdc28p kinase; morphogenesis checkpoint kinase; positive regulator of sphingolipid biosynthesis via Orm2p; phosphorylates a tyrosine residue in the N-terminus of Hsp90 in a cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:9756928]
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:9756928]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:9756928]
Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:22643220]
EXISTENCE:mutant phenotype:GO:0000423 mitophagy [PMID:29673596]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:22643220]
EXISTENCE:physical interaction:GO:0030242 autophagy of peroxisome [PMID:22643220]
Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene
chromatin DNA-binding EKC/KEOPS complex subunit GON7
EXISTENCE:direct assay:GO:0000408 EKC/KEOPS complex [PMID:16564010|PMID:16874308]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16874308]
EXISTENCE:direct assay:GO:0031490 chromatin DNA binding [PMID:16874308]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:14734024]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16874308]
Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; implicated in osmotic stress response; other complex members are Kae1p, Cgi121p, Pcc1p, and Bud32p
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:10635561]
EXISTENCE:direct assay:GO:0006486 protein glycosylation [PMID:10635561]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:10635561]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:23210626]
EXISTENCE:physical interaction:GO:0000136 mannan polymerase complex [PMID:9430634]
Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
hypothetical protein; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; contains a PH-like domain; RBH1 has a paralog, RBH2, that arose from the whole genome duplication
ATP synthase complex assembly protein ATP12
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1826907|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:2142305]
EXISTENCE:physical interaction:GO:0019904 protein domain specific binding [PMID:10747017]
EXISTENCE:physical interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:10747017]
Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:9630229]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:9630229]
EXISTENCE:mutant phenotype:GO:0016272 prefoldin complex [PMID:9630229]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:24068951]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:9630229]
Subunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:17135291]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17135291]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:25557545]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16107716]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12186856]
EXISTENCE:direct assay:GO:0016236 macroautophagy [PMID:25557545]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:12186856]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:17135291]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:12186856]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:17135291]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:17135291]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:17135291]
Type I membrane protein involved in autophagy and the Cvt pathway; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23268442]
EXISTENCE:genetic interaction:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23268442]
Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16461455]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1339306|PMID:22932476]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:8016655]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]
EXISTENCE:mutant phenotype:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:8221926]
EXISTENCE:mutant phenotype:GO:0003677 DNA binding [PMID:16461455]
EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF complex [PMID:8159677]
EXISTENCE:mutant phenotype:GO:0031496 positive regulation of mating type switching [PMID:6436497]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1339306|PMID:3542227]
EXISTENCE:physical interaction:GO:0042393 histone binding [PMID:17496903]
Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:8413233]
EXISTENCE:mutant phenotype:GO:0006077 (1->6)-beta-D-glucan metabolic process [PMID:8413233]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:8413233]
Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9679065]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:7761422]
EXISTENCE:direct assay:GO:0005662 DNA replication factor A complex [PMID:2554144]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:2554144]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:8910442]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:12226081]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:7761422]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:9679065]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:14702040]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:9093844]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:9093844]
EXISTENCE:mutant phenotype:GO:0010833 telomere maintenance via telomere lengthening [PMID:22354040]
EXISTENCE:physical interaction:GO:0000722 telomere maintenance via recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0006312 mitotic recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0007004 telomere maintenance via telomerase [PMID:2554144]
EXISTENCE:physical interaction:GO:0007131 reciprocal meiotic recombination [PMID:2554144]
EXISTENCE:physical interaction:GO:0016567 protein ubiquitination [PMID:18342608]
EXISTENCE:physical interaction:GO:0045184 establishment of protein localization [PMID:2554144]
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:1569061]
EXISTENCE:mutant phenotype:GO:0004180 carboxypeptidase activity [PMID:2026161]
EXISTENCE:mutant phenotype:GO:0006807 nitrogen compound metabolic process [PMID:2026161]
EXISTENCE:mutant phenotype:GO:0051603 proteolysis involved in protein catabolic process [PMID:2026161]
Vacuolar carboxypeptidase S; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via AP-3 pathway; expression induced under low-nitrogen conditions
ARS1006; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
GPI-anchored cell wall hypothetical protein; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11073983]
EXISTENCE:expression pattern:GO:0000747 conjugation with cellular fusion [PMID:10568744]
Protein that regulates signaling from G protein beta subunit Ste4p; contributes to relocalization of Ste4p within the cell; specific to a-cells and induced by alpha-factor
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:28483910]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0006354 DNA-templated transcription elongation [PMID:12736296]
EXISTENCE:direct assay:GO:0006355 regulation of DNA-templated transcription [PMID:11839797]
EXISTENCE:direct assay:GO:0046975 histone H3K36 methyltransferase activity [PMID:11839797]
EXISTENCE:mutant phenotype:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:35263330]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23109428]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11839797]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:19417103]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:17150765]
EXISTENCE:mutant phenotype:GO:0045128 negative regulation of reciprocal meiotic recombination [PMID:18515193]
EXISTENCE:mutant phenotype:GO:0046975 histone H3K36 methyltransferase activity [PMID:11839797]
EXISTENCE:mutant phenotype:GO:0060195 negative regulation of antisense RNA transcription [PMID:21248844]
Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for their deacetylation within transcribed genes; involved in transcription coupled-nucleotide excision repair; associates with C-terminal domain (CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to cytosol in response to hypoxia
bifunctional (2E,6E)-farnesyl diphosphate synthase/dimethylallyltranstransferase
EXISTENCE:direct assay:GO:0004161 dimethylallyltranstransferase activity [PMID:2681213]
EXISTENCE:direct assay:GO:0004337 geranyltranstransferase activity [PMID:2681213]
EXISTENCE:direct assay:GO:0004452 isopentenyl-diphosphate delta-isomerase activity [PMID:7908830]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19416104]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:19416104]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:7908830]
EXISTENCE:direct assay:GO:0008299 isoprenoid biosynthetic process [PMID:2681213]
EXISTENCE:direct assay:GO:0045337 farnesyl diphosphate biosynthetic process [PMID:2681213]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:1779710]
Farnesyl pyrophosphate synthetase; has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
ubiquinol--cytochrome-c reductase subunit 8
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:8394810]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:8786239]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8394810]
Subunit 8 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:32191698]
EXISTENCE:genetic interaction:GO:0008104 protein localization [PMID:21237705]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:10207057]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:32191698]
Snf1p-related nutrient-responsive protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
cAMP-dependent protein kinase catalytic subunit TPK1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:26403272]
EXISTENCE:direct assay:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373|PMID:34244791]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10625697]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10625697]
EXISTENCE:direct assay:GO:0005952 cAMP-dependent protein kinase complex [PMID:12024012]
EXISTENCE:direct assay:GO:0018105 peptidyl-serine phosphorylation [PMID:34244791]
EXISTENCE:genetic interaction:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:genetic interaction:GO:0005952 cAMP-dependent protein kinase complex [PMID:3036373]
EXISTENCE:genetic interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:34244791|PMID:33008596]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:3036373]
EXISTENCE:genetic interaction:GO:0010607 negative regulation of cytoplasmic mRNA processing body assembly [PMID:21925385]
EXISTENCE:genetic interaction:GO:0010737 protein kinase A signaling [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0004691 cAMP-dependent protein kinase activity [PMID:3036373]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:34244791|PMID:33008596]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:34244791]
EXISTENCE:mutant phenotype:GO:0007265 Ras protein signal transduction [PMID:2823100]
EXISTENCE:mutant phenotype:GO:0016241 regulation of macroautophagy [PMID:15016820]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:34244791]
EXISTENCE:physical interaction:GO:0005952 cAMP-dependent protein kinase complex [PMID:12024012]
cAMP-dependent protein kinase catalytic subunit; role in nutrient-stimulated growth via Ras-cAMP signaling; inhibited by regulatory subunit Bcy1p in cAMPs absence; phosphorylates and inhibits Whi3p to promote G1/S phase passage; phosphorylates Nej1p to regulate nonhomologous end joining (NHEJ); impairs mitochondrial import of pre-Tom40p via phosphorylation under non-respiratory conditions; partially redundant with Tpk2p and Tpk3p; human homolog PRKACB regulates NHEJ via NHEJ1 phosphorylation
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
ARS1007; Autonomously Replicating Sequence
hypothetical protein; contains a J-domain, which is a region with homology to the E. coli DnaJ protein
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
hypothetical protein; member of the PIR (Proteins with Internal Repeats) family of cell wall proteins; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, and mCherry fusion localizes to the vacuole; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:10209754]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:19129178]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:16278454]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10209754|PMID:15781460|PMID:9192695]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:21446055]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:21446055]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10407261]
O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:12702350|PMID:19129178]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11914276]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10438739]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10438739|PMID:15781460]
Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1
EXISTENCE:direct assay:GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity [PMID:8066461]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9822386|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9822386|PMID:22842922]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:14988725]
EXISTENCE:direct assay:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
EXISTENCE:mutant phenotype:GO:0000751 mitotic cell cycle G1 arrest in response to pheromone [PMID:2147873]
EXISTENCE:mutant phenotype:GO:0043577 chemotropism [PMID:10087263|PMID:7490290]
EXISTENCE:mutant phenotype:GO:0051457 maintenance of protein location in nucleus [PMID:10655592]
EXISTENCE:physical interaction:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
EXISTENCE:direct assay:GO:0004252 serine-type endopeptidase activity [PMID:29661936|PMID:16778074]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11154269]
EXISTENCE:direct assay:GO:0016485 protein processing [PMID:16778074]
EXISTENCE:genetic interaction:GO:0043200 response to amino acid [PMID:11154269]
EXISTENCE:mutant phenotype:GO:0004252 serine-type endopeptidase activity [PMID:16524914]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:16524914]
EXISTENCE:mutant phenotype:GO:0016540 protein autoprocessing [PMID:29661936]
EXISTENCE:mutant phenotype:GO:0043200 response to amino acid [PMID:11154269]
Serine protease of SPS plasma membrane amino acid sensor system; contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p; other members are Ssy1p and Ptr3p
EXISTENCE:mutant phenotype:GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity [PMID:1322693]
EXISTENCE:mutant phenotype:GO:0006006 glucose metabolic process [PMID:1322693]
Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22593205]
EXISTENCE:mutant phenotype:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
EXISTENCE:mutant phenotype:GO:0140312 cargo adaptor activity [PMID:9700157]
EXISTENCE:physical interaction:GO:0005768 endosome [PMID:9700157]
EXISTENCE:physical interaction:GO:0030906 retromer, cargo-selective complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:9700157]
EXISTENCE:physical interaction:GO:0140312 cargo adaptor activity [PMID:9700157]
Endosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p; overexpression of wild-type human VPS35 or Parkinson's-associated vps35-D686N or vps35-P299S variants complements Ni2+ resistance and Cd2+ sensitivity of yeast vps35 null mutant
EXISTENCE:direct assay:GO:0004512 inositol-3-phosphate synthase activity [PMID:7016881]
EXISTENCE:mutant phenotype:GO:0006021 inositol biosynthetic process [PMID:7016881]
Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:22761830|PMID:23708375]
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:12499386|PMID:17182850]
EXISTENCE:direct assay:GO:0031625 ubiquitin protein ligase binding [PMID:17182849|PMID:17332499]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:19944104]
EXISTENCE:mutant phenotype:GO:0031625 ubiquitin protein ligase binding [PMID:17182849|PMID:17332499]
EXISTENCE:mutant phenotype:GO:0032509 endosome transport via multivesicular body sorting pathway [PMID:22212814]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:17182849|PMID:17332499]
Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
SCF ubiquitin ligase complex subunit DAS1
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:11283612|PMID:14747994]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:2850487]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22266655|PMID:31804585]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:7557394]
EXISTENCE:genetic interaction:GO:0019843 rRNA binding [PMID:7557394]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:2406561|PMID:7557394]
EXISTENCE:mutant phenotype:GO:0019843 rRNA binding [PMID:7557394]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567|PMID:11081632]
C/D box small nucleolar RNA (snoRNA); referred to as U14; essential gene required for cleavage of 35S primary rRNA transcript to release the precursor to the 18S rRNA; guides 2'-O-methylation of small subunit (SSU) rRNA at position C414
C/D box small nucleolar RNA (snoRNA); predicted to guide 2'-O-methylation of large subunit (LSU) rRNA at position G2395; not shown to be active
DNA-directed RNA polymerase I subunit RPA34
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12206772]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12206772]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9121426|PMID:9837969]
EXISTENCE:direct assay:GO:0006362 transcription elongation by RNA polymerase I [PMID:18160037]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:9121426]
EXISTENCE:mutant phenotype:GO:0005736 RNA polymerase I complex [PMID:9121426]
RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Translational repressor of the mitochondrial ATP6/8 mRNA; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:30516470]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:7565676]
EXISTENCE:mutant phenotype:GO:0061077 chaperone-mediated protein folding [PMID:30516470]
Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19477927|PMID:12869188]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19477927]
EXISTENCE:direct assay:GO:0008526 phosphatidylinositol transfer activity [PMID:10848624]
EXISTENCE:direct assay:GO:0015914 phospholipid transport [PMID:10848624]
EXISTENCE:direct assay:GO:0020037 heme binding [PMID:32780017]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:16262726]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:12869188|PMID:26928762]
EXISTENCE:genetic interaction:GO:0017157 regulation of exocytosis [PMID:19477927]
EXISTENCE:genetic interaction:GO:0043001 Golgi to plasma membrane protein transport [PMID:10848624]
EXISTENCE:genetic interaction:GO:0046488 phosphatidylinositol metabolic process [PMID:16262726]
EXISTENCE:genetic interaction:GO:2000114 regulation of establishment of cell polarity [PMID:19477927]
Unusual phosphatidylinositol transfer protein (PITP) that binds heme; similar to Sec14p; founding member of conserved class of fungal hemoproteins; exhibits PI- but not PC-transfer activity; localizes to peripheral endoplasmic reticulum, cytosol, and microsomes; partially relocalizes to plasma membrane upon DNA replication stress
EXISTENCE:mutant phenotype:GO:0042631 cellular response to water deprivation [PMID:21420397]
EXISTENCE:mutant phenotype:GO:0055106 ubiquitin-protein transferase regulator activity [PMID:29861160]
EXISTENCE:mutant phenotype:GO:0120174 stress-induced homeostatically regulated protein degradation pathway [PMID:29861160]
EXISTENCE:physical interaction:GO:0055106 ubiquitin-protein transferase regulator activity [PMID:29861160]
Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:15618217]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:18826960]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:11344168|PMID:15618217]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:15618217|PMID:7600576|PMID:7919535|PMID:11344168]
EXISTENCE:physical interaction:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:7600576]
Essential component of the TIM23 complex; with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core Translocase of the Inner Mitochondrial membrane (TIM23 complex)
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:18085829]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:23357952]
hypothetical protein; partially overlaps verified gene YAK1/YJL141C but does not share all phenotypes; null mutant displays increased levels of spontaneous Rad52p foci, increased sporulation efficiency, and small defect in vacuolar fragmentation
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:2072907|PMID:24140345|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:21255108]
EXISTENCE:direct assay:GO:0004713 protein tyrosine kinase activity [PMID:21255108]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21255108|PMID:11358866]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21255108|PMID:11358866]
EXISTENCE:genetic interaction:GO:0010737 protein kinase A signaling [PMID:8065298]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24140345]
EXISTENCE:genetic interaction:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:24140345]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:21255108]
EXISTENCE:mutant phenotype:GO:0004713 protein tyrosine kinase activity [PMID:21255108]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24140345]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:24140345]
Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose
DNA-directed RNA polymerase II subunit RPB4
EXISTENCE:direct assay:GO:0000932 P-body [PMID:16357218]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:11087726]
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:11087726]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12857861]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12857861]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:11087726]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:16357218]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:11577101]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:12857861]
EXISTENCE:mutant phenotype:GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [PMID:18195044]
EXISTENCE:mutant phenotype:GO:0045948 positive regulation of translational initiation [PMID:21074047]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:21074047]
RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
EXISTENCE:direct assay:GO:0000030 mannosyltransferase activity [PMID:8631921]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8631921]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:8631921]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:8631921]
Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:1502180]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:1502180]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:1502180]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:24471916]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:29192585|PMID:30281017]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:1502180]
EXISTENCE:mutant phenotype:GO:0016281 eukaryotic translation initiation factor 4F complex [PMID:10409745|PMID:2648398]
Translation initiation factor eIF4A; DEAD-box RNA helicase that couples ATPase activity to mRNA binding and unwinding; ATP hydrolysis accelerates the recruitment of mRNA during 48S preinitiation complex (PIC) assembly; ATPase activity stimulated by either eIF4G1 or by the PIC in an eIF3g and eIFGi subunit-dependent manner, independent of eIF4E and eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E and eIF4G; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0005978 glycogen biosynthetic process [PMID:8524228]
EXISTENCE:genetic interaction:GO:0008466 glycogenin glucosyltransferase activity [PMID:8524228]
Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
hypothetical protein; identified by SAGE
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:3533916]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10477278|PMID:26928762]
EXISTENCE:direct assay:GO:0042392 sphingosine-1-phosphate phosphatase activity [PMID:9353337]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:11278643]
Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
Putative mitochondrial hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1703236]
EXISTENCE:direct assay:GO:0048250 iron import into the mitochondrion [PMID:12902335]
EXISTENCE:mutant phenotype:GO:0005381 iron ion transmembrane transporter activity [PMID:12902335]
Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11935221]
hypothetical protein; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:30927524]
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:23954798]
Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; interacts with mitochondrial ribosomal small subunit; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase
EXISTENCE:direct assay:GO:0004070 aspartate carbamoyltransferase activity [PMID:10446140]
EXISTENCE:direct assay:GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [PMID:5776390]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11015727]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:181668]
EXISTENCE:direct assay:GO:0006541 glutamine metabolic process [PMID:5651325]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11921093]
EXISTENCE:direct assay:GO:0045984 negative regulation of pyrimidine nucleobase metabolic process [PMID:5651325]
EXISTENCE:mutant phenotype:GO:0004070 aspartate carbamoyltransferase activity [PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:4550660]
Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16166647]
EXISTENCE:direct assay:GO:0015079 potassium ion transmembrane transporter activity [PMID:3043197]
EXISTENCE:direct assay:GO:0030007 cellular potassium ion homeostasis [PMID:3043197]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:16166647]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:33640956]
Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
mitogen-activated protein kinase kinase PBS2
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9755161]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10980703]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10980703]
EXISTENCE:genetic interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:7624781]
EXISTENCE:genetic interaction:GO:0071474 cellular hyperosmotic response [PMID:7624781]
EXISTENCE:mutant phenotype:GO:0004708 MAP kinase kinase activity [PMID:7624781]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:15707964]
EXISTENCE:mutant phenotype:GO:0006972 hyperosmotic response [PMID:7681220]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:7941729]
EXISTENCE:mutant phenotype:GO:0007231 osmosensory signaling pathway [PMID:7624781]
EXISTENCE:physical interaction:GO:0005078 MAP-kinase scaffold activity [PMID:15200958|PMID:15200959|PMID:9180081]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:14685261]
MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
Mitochondrial hypothetical protein; identified based on homology to the filamentous fungus, Ashbya gossypii; SWAT-GFP and seamless-GFP fusion proteins localize to the mitochondria
EXISTENCE:curator inference:GO:0032931 histone H3K56 acetyltransferase activity [PMID:15882620]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:16199888]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:15882620]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21057056]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:26649943|PMID:17526727]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:21057056]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:17078097]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:12192040]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:21057056]
EXISTENCE:mutant phenotype:GO:0097043 histone H3-K56 acetylation [PMID:15882620]
Histone H3 acetylase with a role in transcriptional regulation; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near TATA box; involved in S phase-specific acetylation of H3K56 at histone promoters, which is required for recruitment of SWI/SNF nucleosome remodeling complex and subsequent transcription
EXISTENCE:direct assay:GO:0043605 amide catabolic process [PMID:28373563]
EXISTENCE:direct assay:GO:0110050 deaminated glutathione amidase activity [PMID:28373563]
Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
tRNA 1-methyladenosine methyltransferase subunit GCD14
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9851972]
EXISTENCE:direct assay:GO:0016429 tRNA (adenine-N1-)-methyltransferase activity [PMID:10779558]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:10779558]
EXISTENCE:direct assay:GO:0031515 tRNA (m1A) methyltransferase complex [PMID:10779558]
EXISTENCE:mutant phenotype:GO:0016429 tRNA (adenine-N1-)-methyltransferase activity [PMID:10779558]
EXISTENCE:physical interaction:GO:0031515 tRNA (m1A) methyltransferase complex [PMID:10779558]
Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
EXISTENCE:direct assay:GO:0000932 P-body [PMID:12730603|PMID:18611963]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23706738]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18029398|PMID:23706738|PMID:22842922]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:10747033]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:10761922]
EXISTENCE:mutant phenotype:GO:0000339 RNA cap binding [PMID:11741542]
EXISTENCE:mutant phenotype:GO:0000932 P-body [PMID:12730603]
Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31323700]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
hypothetical protein that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16651379|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16651379]
EXISTENCE:genetic interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:16651379]
EXISTENCE:physical interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:16651379]
Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
EXISTENCE:direct assay:GO:0004750 D-ribulose-phosphate 3-epimerase activity [PMID:8929392]
EXISTENCE:mutant phenotype:GO:0004750 D-ribulose-phosphate 3-epimerase activity [PMID:8929392]
EXISTENCE:mutant phenotype:GO:0006098 pentose-phosphate shunt [PMID:8929392]
D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
hypothetical protein; may interact with ribosomes, based on co-purification experiments; YJL118W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10655492|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15623581]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10655492]
EXISTENCE:mutant phenotype:GO:0010966 regulation of phosphate transport [PMID:10655492]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:15623581]
Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0042776 proton motive force-driven mitochondrial ATP synthesis [PMID:7586026]
Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the vacuole; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11404324|PMID:22932476]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:16303565]
EXISTENCE:direct assay:GO:0006335 DNA replication-dependent chromatin assembly [PMID:11412995]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:22308335]
EXISTENCE:direct assay:GO:0035066 positive regulation of histone acetylation [PMID:17272722|PMID:18458063]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:15840725]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:15840725]
EXISTENCE:genetic interaction:GO:0035066 positive regulation of histone acetylation [PMID:18458063]
EXISTENCE:mutant phenotype:GO:0001932 regulation of protein phosphorylation [PMID:27222517]
EXISTENCE:mutant phenotype:GO:0006337 nucleosome disassembly [PMID:16678113]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19620280]
EXISTENCE:mutant phenotype:GO:0010468 regulation of gene expression [PMID:26941319]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:16627621]
EXISTENCE:mutant phenotype:GO:0033523 histone H2B ubiquitination [PMID:24824343]
EXISTENCE:mutant phenotype:GO:0035066 positive regulation of histone acetylation [PMID:17272722|PMID:17679090|PMID:17690098|PMID:18458063]
EXISTENCE:mutant phenotype:GO:0042393 histone binding [PMID:15840725]
EXISTENCE:physical interaction:GO:0035066 positive regulation of histone acetylation [PMID:17690098]
Nucleosome assembly factor; involved in chromatin assembly, disassembly; required for recovery after DSB repair; role in H3K56 acetylation required for expression homeostasis, buffering mRNA synthesis rate against gene dosage changes in S phase; anti-silencing protein, derepresses silent loci when overexpressed; role in regulating Ty1 transposition; relocalizes to cytosol under hypoxia; growth defect of asf1 null is functionally complemented by either human ASF1A or ASF1B
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
YJLWTy4-1; Ty4 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
ARS1008; Autonomously Replicating Sequence
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:11038180|PMID:11038182|PMID:16407407]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:genetic interaction:GO:0000266 mitochondrial fission [PMID:11179417]
EXISTENCE:genetic interaction:GO:0016559 peroxisome fission [PMID:18445678]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:11038182]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:11179417|PMID:11038180|PMID:11038182|PMID:11038183]
EXISTENCE:physical interaction:GO:0000266 mitochondrial fission [PMID:11038182|PMID:11179417]
Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the self-assembled oligomeric form of the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
chaperonin-containing T-complex subunit CCT7
EXISTENCE:direct assay:GO:0005832 chaperonin-containing T-complex [PMID:16762366]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:16762366]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16762366]
EXISTENCE:physical interaction:GO:0005832 chaperonin-containing T-complex [PMID:15704212]
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:19380492]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19380492]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:9171383]
EXISTENCE:genetic interaction:GO:0090295 nitrogen catabolite repression of transcription [PMID:9106207]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9171383]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:19380492]
EXISTENCE:mutant phenotype:GO:0090295 nitrogen catabolite repression of transcription [PMID:9171383|PMID:9171427]
EXISTENCE:physical interaction:GO:0051457 maintenance of protein location in nucleus [PMID:19380492]
GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
snoRNA-binding rRNA-processing protein UTP10
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:17652137|PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:17652137|PMID:15167894]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17652137]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17652137]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17652137]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
Pheromone-regulated protein; proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation
hypothetical protein; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:7764269|PMID:16319894]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7764269]
EXISTENCE:genetic interaction:GO:1902596 negative regulation of DNA replication origin binding [PMID:29388912]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:9003314]
EXISTENCE:mutant phenotype:GO:1902596 negative regulation of DNA replication origin binding [PMID:29388912]
Serine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p; human CDK2 can complement ime2 null mutant
EXISTENCE:direct assay:GO:0035065 regulation of histone acetylation [PMID:34625508]
EXISTENCE:mutant phenotype:GO:1900407 regulation of cellular response to oxidative stress [PMID:30082318]
Chromatin-associated protein involved in control of stress response; regulates response to oxidative stress; involved in control of gene repression in subtelomeric chromatin; contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:14981506|PMID:14981507]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:14981506]
EXISTENCE:direct assay:GO:0019904 protein domain specific binding [PMID:16105940]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:14981506|PMID:14981507]
Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:35385737]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748|PMID:35385737]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:35385737]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA) involved in ribosome synthesis; has structural role in early pre-60S pathway; guides pseudouridylation of large subunit (LSU) rRNA at position U2944
ARS1009; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:16785442]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:16785442]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16785442]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:16785442]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16785442]
Putative zinc cluster hypothetical protein; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:21414316]
EXISTENCE:mutant phenotype:GO:0051881 regulation of mitochondrial membrane potential [PMID:21414316]
Mitochondrial elongation factor involved in translational elongation
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0004357 glutamate-cysteine ligase activity [PMID:1687097]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:2184310]
EXISTENCE:direct assay:GO:0006750 glutathione biosynthetic process [PMID:1687097]
EXISTENCE:direct assay:GO:0046686 response to cadmium ion [PMID:10921921]
EXISTENCE:mutant phenotype:GO:0004357 glutamate-cysteine ligase activity [PMID:2184310]
EXISTENCE:mutant phenotype:GO:0006750 glutathione biosynthetic process [PMID:8768524]
EXISTENCE:mutant phenotype:GO:0042542 response to hydrogen peroxide [PMID:7615092]
Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12361950|PMID:12523934|PMID:26928762]
EXISTENCE:direct assay:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:12361950|PMID:12523934]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12361950|PMID:12523934]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:12523934|PMID:12361950]
EXISTENCE:mutant phenotype:GO:0004430 1-phosphatidylinositol 4-kinase activity [PMID:12361950]
EXISTENCE:mutant phenotype:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:12361950]
Type II phosphatidylinositol 4-kinase; binds Las17p, a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
EXISTENCE:direct assay:GO:0030140 trans-Golgi network transport vesicle [PMID:16498409]
EXISTENCE:direct assay:GO:0034044 exomer complex [PMID:17000877|PMID:23000721]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:11879634|PMID:9614194]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:9614194]
Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:1903766 positive regulation of potassium ion export across plasma membrane [PMID:15947196]
EXISTENCE:genetic interaction:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8649382]
EXISTENCE:mutant phenotype:GO:1903766 positive regulation of potassium ion export across plasma membrane [PMID:15947196]
Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14690591]
EXISTENCE:direct assay:GO:0004300 enoyl-CoA hydratase activity [PMID:18272525]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:18272525]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:14690591]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:16943325]
EXISTENCE:mutant phenotype:GO:0018812 3-hydroxyacyl-CoA dehydratase activity [PMID:23416297]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:18272525]
EXISTENCE:mutant phenotype:GO:0030497 fatty acid elongation [PMID:23416297]
Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
mitochondrial 54S ribosomal protein YmL49
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit
ETC3; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; located between MRPL49 and BCK1
mitogen-activated protein kinase kinase kinase BCK1
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:1729597|PMID:8386320]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:1729597]
EXISTENCE:mutant phenotype:GO:0004709 MAP kinase kinase kinase activity [PMID:11283616]
EXISTENCE:mutant phenotype:GO:0010447 response to acidic pH [PMID:16087742]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:1545797]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16380504]
EXISTENCE:mutant phenotype:GO:0035556 intracellular signal transduction [PMID:8190082]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:1729597]
MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15926887]
EXISTENCE:mutant phenotype:GO:0015386 potassium:proton antiporter activity [PMID:9811642]
EXISTENCE:mutant phenotype:GO:0098662 inorganic cation transmembrane transport [PMID:9811642]
Putative K+/H+ antiporter; involved in intracellular cation homeostasis; promotes copper binding to Fet3p multicopper oxidase; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005267 potassium channel activity [PMID:7651518]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7651518]
EXISTENCE:direct assay:GO:0030007 cellular potassium ion homeostasis [PMID:7651518]
EXISTENCE:mutant phenotype:GO:0005249 voltage-gated potassium channel activity [PMID:16133265]
EXISTENCE:mutant phenotype:GO:0005267 potassium channel activity [PMID:7651518]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:7651518]
EXISTENCE:mutant phenotype:GO:0030007 cellular potassium ion homeostasis [PMID:7651518]
Outward-rectifier potassium channel of the plasma membrane; has two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19506039]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:10908335]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:25765656]
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:25765656]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:18440969]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:8419328]
EXISTENCE:direct assay:GO:1990986 DNA recombinase disassembly [PMID:12748645|PMID:12748644]
EXISTENCE:direct assay:GO:2000042 negative regulation of double-strand break repair via homologous recombination [PMID:19595720]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:7768432]
EXISTENCE:mutant phenotype:GO:0042262 DNA protection [PMID:31168653]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:23770697]
EXISTENCE:mutant phenotype:GO:1990986 DNA recombinase disassembly [PMID:23770697]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:31142613]
DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1
glucosaminyl-phosphotidylinositol O-acyltransferase
EXISTENCE:direct assay:GO:0006506 GPI anchor biosynthetic process [PMID:12714589]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:21367863]
EXISTENCE:genetic interaction:GO:0032216 glucosaminyl-phosphatidylinositol O-acyltransferase activity [PMID:14517336]
EXISTENCE:mutant phenotype:GO:0032216 glucosaminyl-phosphatidylinositol O-acyltransferase activity [PMID:16361252]
Protein involved in the inositol acylation of GlcN-PI; the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) forms glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
EXISTENCE:direct assay:GO:0000725 recombinational repair [PMID:16840526]
EXISTENCE:direct assay:GO:0001934 positive regulation of protein phosphorylation [PMID:18842580|PMID:18922789]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:10733584]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:18922789]
EXISTENCE:direct assay:GO:0070182 DNA polymerase binding [PMID:10733584]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:16840526]
EXISTENCE:genetic interaction:GO:0007095 mitotic G2 DNA damage checkpoint signaling [PMID:18541674]
EXISTENCE:mutant phenotype:GO:0000076 DNA replication checkpoint signaling [PMID:10097122]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:10733584|PMID:23629628]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:21130053]
EXISTENCE:mutant phenotype:GO:0031261 DNA replication preinitiation complex [PMID:17167415]
EXISTENCE:mutant phenotype:GO:1902597 positive regulation of DNA replication origin binding [PMID:10733584]
DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11405098|PMID:14685767|PMID:9843506]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:14685767]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11331606|PMID:9843506]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:25123508]
EXISTENCE:genetic interaction:GO:0061414 positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:25123508|PMID:9843506]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9843506]
EXISTENCE:mutant phenotype:GO:0061414 positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:9843506]
EXISTENCE:mutant phenotype:GO:2000218 negative regulation of invasive growth in response to glucose limitation [PMID:11095711]
EXISTENCE:physical interaction:GO:0045722 positive regulation of gluconeogenesis [PMID:9843506]
C6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
EXISTENCE:direct assay:GO:0004585 ornithine carbamoyltransferase activity [PMID:7029528]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:205532]
EXISTENCE:mutant phenotype:GO:0006526 arginine biosynthetic process [PMID:2897249]
Ornithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline
EXISTENCE:direct assay:GO:0003972 RNA ligase (ATP) activity [PMID:3277966|PMID:3512545]
EXISTENCE:direct assay:GO:0004113 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity [PMID:6297798]
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:3312232]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20844078]
EXISTENCE:direct assay:GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation [PMID:2207062]
EXISTENCE:direct assay:GO:0051730 GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity [PMID:8428918]
EXISTENCE:mutant phenotype:GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation [PMID:1537841]
EXISTENCE:mutant phenotype:GO:0032056 positive regulation of translation in response to stress [PMID:20844078]
EXISTENCE:mutant phenotype:GO:0036498 IRE1-mediated unfolded protein response [PMID:8898194]
EXISTENCE:mutant phenotype:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:31913314]
EXISTENCE:mutant phenotype:GO:2000622 regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:30874502]
tRNA ligase; required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; involved in turnover of tRNA introns; localized at the inner nuclear envelope and cytoplasm
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:18195105]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:8978675]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:17717527]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17717527]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10207081|PMID:17717527|PMID:18946089|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:18946089|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0007266 Rho protein signal transduction [PMID:19955214]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:10588647]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:15583030|PMID:17717527]
EXISTENCE:genetic interaction:GO:0007266 Rho protein signal transduction [PMID:19955214]
EXISTENCE:genetic interaction:GO:0051601 exocyst localization [PMID:17717527|PMID:18946089]
EXISTENCE:mutant phenotype:GO:0001927 exocyst assembly [PMID:15583030|PMID:18946089]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:17339375]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:17339375]
EXISTENCE:physical interaction:GO:0007266 Rho protein signal transduction [PMID:19955214]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:10207081|PMID:19955214]
Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:20739461]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:20739461]
EXISTENCE:mutant phenotype:GO:0032386 regulation of intracellular transport [PMID:20739461]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:32800549]
EXISTENCE:mutant phenotype:GO:2000397 positive regulation of ubiquitin-dependent endocytosis [PMID:20956561]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:20956561]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Alpha arrestin, Ub-ligase adaptor for Rsp5p; controls nutrient limitation-mediated endosomal recycling of the Gap1p amino acid permease; PY motif-containing adaptor involved in substrate-induced, Rsp5p-mediated ubiquitination and endocytosis of select plasma membrane localized amino acid transporters, such as Dip5p and Put4p; phosphorylated by Npr1p and by the Pcl7p-Pho85p cyclin-CDK complex; interacts with AP-1 subunit Apl4p
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:18298591]
EXISTENCE:genetic interaction:GO:0006914 autophagy [PMID:18298591]
EXISTENCE:genetic interaction:GO:0009267 cellular response to starvation [PMID:18298591]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:15265867]
EXISTENCE:genetic interaction:GO:0048017 inositol lipid-mediated signaling [PMID:15265867]
EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:mutant phenotype:GO:0071218 cellular response to misfolded protein [PMID:26004510]
Protein required for clearance of inclusion bodies; localizes to the inclusion bodies formed under protein misfolding stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10436015]
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608|PMID:16299513]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10436015]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:16088870]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10911987|PMID:11011149]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:12353039|PMID:16135807]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10911987]
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:10911987]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:10911987]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:10911987]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15610740]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:10436015]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:8598290]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:17452364]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:10952318|PMID:24034245]
Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11278502]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:22832273|PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23222640]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11278502]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:11278502]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:8533468]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:14536090]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:16632467]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8533468]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16632467]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16632467]
EXISTENCE:mutant phenotype:GO:0043577 chemotropism [PMID:22832273]
EXISTENCE:mutant phenotype:GO:0045141 meiotic telomere clustering [PMID:16632467]
EXISTENCE:physical interaction:GO:0001965 G-protein alpha-subunit binding [PMID:14536090]
Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
EXISTENCE:direct assay:GO:0000287 magnesium ion binding [PMID:27344972]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0005504 fatty acid binding [PMID:28365570]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11935221]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11914276]
EXISTENCE:direct assay:GO:0015485 cholesterol binding [PMID:27344972]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:23027975]
EXISTENCE:genetic interaction:GO:0015908 fatty acid transport [PMID:28365570]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:23027975]
EXISTENCE:mutant phenotype:GO:0005504 fatty acid binding [PMID:28365570]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:23027975]
EXISTENCE:mutant phenotype:GO:0032934 sterol binding [PMID:23027975]
Lipid binding protein; binds to both free fatty acids with preference for saturated long-chain fatty acids and to sterols; contains two independent lipid-binding sites; involved in the export of free fatty acids and acetylated sterols; export functions are redundant with PRY2, and PRY3; important for survival of cells that accumulate free fatty acids; involved in detoxification of hydrophobic compounds; secreted glycoprotein and member of the CAP/SCP protein superfamily
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953|PMID:15781460|PMID:32554483]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:32554483]
GPI-anchored cell wall protein involved in export of sterols; redundant role with PRY1 and PRY2 in the export of free fatty acids; role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins)
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:mutant phenotype:GO:0055070 copper ion homeostasis [PMID:26127016]
Protein with a role in copper homeostasis; possible role in vacuolar sorting and processing of secretory proteins; null mutants are hypersensitive to sortin2
hypothetical protein; mRNA identified as translated by ribosome profiling data; completely overlaps the verified gene YJL077C/ICS3
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:10219245]
EXISTENCE:direct assay:GO:0004864 protein phosphatase inhibitor activity [PMID:10219244]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10219244]
EXISTENCE:direct assay:GO:0030869 RENT complex [PMID:10219244]
EXISTENCE:direct assay:GO:1902570 protein localization to nucleolus [PMID:33481703]
EXISTENCE:genetic interaction:GO:0001100 negative regulation of exit from mitosis [PMID:10219244]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:10219245]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:10219244|PMID:26090959]
EXISTENCE:mutant phenotype:GO:0007000 nucleolus organization [PMID:10219245]
EXISTENCE:mutant phenotype:GO:1904751 positive regulation of protein localization to nucleolus [PMID:10219244]
Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
hypothetical protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps verified gene NET1, but does not share all phenotypes; null mutant is slow-growing, sensitive to heat, sorbate, and other various chemicals
ARS1010; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:17055978]
EXISTENCE:direct assay:GO:0030892 mitotic cohesin complex [PMID:9990856]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:9335333]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:34897432]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:10412984]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10412984]
EXISTENCE:mutant phenotype:GO:0051177 meiotic sister chromatid cohesion [PMID:10412984]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:16888651]
EXISTENCE:physical interaction:GO:0019901 protein kinase binding [PMID:27325700]
Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9148890|PMID:26928762]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:15282802]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:9817751]
EXISTENCE:genetic interaction:GO:0051087 chaperone binding [PMID:9148890]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:10069807]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11381090]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:9148890]
DnaJ-like chaperone required for nuclear membrane fusion during mating; localizes to the ER membrane; exhibits genetic interactions with KAR2
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:genetic interaction:GO:0006261 DNA-templated DNA replication [PMID:12730134]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:12768207]
EXISTENCE:physical interaction:GO:0000811 GINS complex [PMID:12768207|PMID:12730134]
EXISTENCE:physical interaction:GO:0031261 DNA replication preinitiation complex [PMID:12730134]
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
acetyl-CoA:L-glutamate N-acetyltransferase
EXISTENCE:direct assay:GO:0004042 acetyl-CoA:L-glutamate N-acetyltransferase activity [PMID:11553611]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:205532]
EXISTENCE:mutant phenotype:GO:0004042 acetyl-CoA:L-glutamate N-acetyltransferase activity [PMID:11553611]
EXISTENCE:mutant phenotype:GO:0006592 ornithine biosynthetic process [PMID:11553611]
Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
metallo-dependent hydrolase superfamily protein
Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835|PMID:14562095]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0034388 Pwp2p-containing subcomplex of 90S preribosome [PMID:15231838|PMID:17515605]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
Small-subunit processome protein involved in pre-18S rRNA maturation; part of a subunit of the 90S preribosomal particle capable of interacting directly with the 5' ETS of the 35S pre-rRNA; contains WD40 repeats
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10427036]
EXISTENCE:direct assay:GO:0018738 S-formylglutathione hydrolase activity [PMID:10427036]
EXISTENCE:mutant phenotype:GO:0046294 formaldehyde catabolic process [PMID:10427036]
Esterase that can function as an S-formylglutathione hydrolase; non-essential intracellular esterase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289|PMID:22993211]
Mitochondrial intermembrane space hypothetical protein
EXISTENCE:direct assay:GO:0008623 CHRAC [PMID:15024052]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:14673157]
Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication
mitochondrial 54S ribosomal protein YmL8
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:21068384|PMID:20876281]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:21068384|PMID:20876281]
EXISTENCE:mutant phenotype:GO:0070130 negative regulation of mitochondrial translation [PMID:21068384|PMID:20876281]
Mitochondrial protein required for cytochrome c oxidase assembly; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; located in the mitochondrial inner membrane
mannose-ethanolamine phosphotransferase LAS21
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10329735]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10329735]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:15452134|PMID:10329735]
EXISTENCE:mutant phenotype:GO:0051267 CP2 mannose-ethanolamine phosphotransferase activity [PMID:10329735|PMID:15452134]
Mannose-ethanolamine phosphotransferase; involved in biosynthesis of the glycosylphosphatidylinositol (GPI) core structure, catalyzing the addition of a side chain ethanolamine phosphate to the alpha1,6-linked second mannose residue of the GPI lipid precursor; integral component of endoplasmic reticulum membrane; mutations affect cell wall integrity; homolog of human PIGG
EXISTENCE:curator inference:GO:0017056 structural constituent of nuclear pore [PMID:18046406]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:7559751]
EXISTENCE:direct assay:GO:0044612 nuclear pore linkers [PMID:18046406]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:10684247|PMID:9736720]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906|PMID:11739405]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:10465789|PMID:11739405]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:7559750|PMID:7559751]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:15294903]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:15294903|PMID:17347149]
Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17034789|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17034789|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0016212 kynurenine-oxoglutarate transaminase activity [PMID:18205391]
EXISTENCE:direct assay:GO:0034276 kynurenic acid biosynthetic process [PMID:18205391]
EXISTENCE:direct assay:GO:0047536 2-aminoadipate transaminase activity [PMID:19342587]
Kynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:10319861|PMID:9753630]
EXISTENCE:mutant phenotype:GO:0015819 lysine transport [PMID:14660799]
EXISTENCE:mutant phenotype:GO:0051453 regulation of intracellular pH [PMID:10191121|PMID:10319861|PMID:16423829]
EXISTENCE:mutant phenotype:GO:1903826 L-arginine transmembrane transport [PMID:14660799]
Protein required for the ATP-dependent transport of arginine; vacuolar membrane protein; involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; human homolog CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) can complement yeast null mutant
EXISTENCE:curator inference:GO:0038203 TORC2 signaling [PMID:14736892]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19144819]
EXISTENCE:direct assay:GO:0031932 TORC2 complex [PMID:14736892]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:14736892]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:14736892]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:14736892]
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; PM retention is dependent on Avo3p; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:9786867]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:22128164]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10899124]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:14517251|PMID:16720702]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22128164]
EXISTENCE:mutant phenotype:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:9786867|PMID:12549926]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9271382|PMID:9786867]
EXISTENCE:mutant phenotype:GO:0008270 zinc ion binding [PMID:16720702]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12095998]
Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
EXISTENCE:mutant phenotype:GO:0014074 response to purine-containing compound [PMID:15932646]
hypothetical protein; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:12637749]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:10648604|PMID:10637294]
EXISTENCE:genetic interaction:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10637294|PMID:9412462]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:17893242|PMID:9412462]
Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:9700157]
EXISTENCE:mutant phenotype:GO:0030906 retromer, cargo-selective complex [PMID:9700157]
EXISTENCE:mutant phenotype:GO:0140318 protein transporter activity [PMID:9700157]
EXISTENCE:physical interaction:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0140318 protein transporter activity [PMID:9700157]
Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
ARS1011; Autonomously Replicating Sequence
hypothetical protein; identified based on comparison to related yeast species; mCherry fusion protein localizes to the vacuole
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH1
EXISTENCE:direct assay:GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity [PMID:3905788]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11158358|PMID:26928762]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11158358]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:expression pattern:GO:0006094 gluconeogenesis [PMID:3905788]
EXISTENCE:expression pattern:GO:0006096 glycolytic process [PMID:3905788]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:13679573]
Bud tip localized hypothetical protein; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15226447|PMID:8756671]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16541108]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:18096702]
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:15828860|PMID:15935758|PMID:15935759]
EXISTENCE:direct assay:GO:0034458 3'-5' RNA helicase activity [PMID:18000032|PMID:18096702]
EXISTENCE:direct assay:GO:0043629 ncRNA polyadenylation [PMID:15828860|PMID:15935758]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:15935758|PMID:17643380]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:11586364]
EXISTENCE:genetic interaction:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:18000032]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10508172|PMID:9463390]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0016075 rRNA catabolic process [PMID:10508172|PMID:9463390]
EXISTENCE:mutant phenotype:GO:0034458 3'-5' RNA helicase activity [PMID:18000032]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172|PMID:10611222]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing [PMID:17410208]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:16374505|PMID:18940861]
EXISTENCE:mutant phenotype:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:19369424]
EXISTENCE:mutant phenotype:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10611222]
EXISTENCE:physical interaction:GO:0031499 TRAMP complex [PMID:15935759]
RNA duplex-sensing translocase; ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
Yeast homolog of human CHMP7, localizes to the endoplasmic reticulum presumably as part of an ESCRT-III like complex; null mutant has growth defect at 37 C in an apq12 deletion background; non-essential gene
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14755638]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:14755638]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:14755638]
UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at positions A817 and G908
EXISTENCE:curator inference:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:12676951]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:12676951]
EXISTENCE:direct assay:GO:0035361 Cul8-RING ubiquitin ligase complex [PMID:20139071]
EXISTENCE:mutant phenotype:GO:0007088 regulation of mitotic nuclear division [PMID:12676951]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:0031297 replication fork processing [PMID:18704118]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19390089|PMID:22505030]
Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; Rtt101p-Mms22p ligase associates with replisome complex during S phase via Ctf4p; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19570983]
EXISTENCE:mutant phenotype:GO:0009249 protein lipoylation [PMID:19570983]
Lipoate-protein ligase; octanoyl-CoA: protein transferase required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921|PMID:9730279]
EXISTENCE:genetic interaction:GO:0045333 cellular respiration [PMID:16232921|PMID:9730279]
EXISTENCE:mutant phenotype:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:16232921]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:16232921]
Minor succinate dehydrogenase isozyme; participates in oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner; YJL045W has a paralog, SDH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:8441469]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:35134079]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14610088]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:24924636]
EXISTENCE:direct assay:GO:0090630 activation of GTPase activity [PMID:8441469]
GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport
hypothetical protein; YJL043W is a non-essential gene
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:9855114]
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:8947554]
EXISTENCE:mutant phenotype:GO:0007026 negative regulation of microtubule depolymerization [PMID:9855114]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9090049]
Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:2112428]
EXISTENCE:direct assay:GO:0017056 structural constituent of nuclear pore [PMID:19680227|PMID:19098896]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:24574455|PMID:9813081|PMID:18046406]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:9813081]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:10952996]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906|PMID:11739405|PMID:9971735]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:8524308]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220|PMID:2112428]
EXISTENCE:mutant phenotype:GO:0097064 ncRNA export from nucleus [PMID:11352936]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:12169684|PMID:17418788|PMID:19098896]
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p)
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0044611 nuclear pore inner ring [PMID:18046406]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:10428845|PMID:11121302]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:10428845]
EXISTENCE:mutant phenotype:GO:0046822 regulation of nucleocytoplasmic transport [PMID:23499021]
Essential subunit of inner ring of nuclear pore complex (NPC); plays a role in modulating transport through the NPC; homologous to human NUP205
ARS1012; Autonomously Replicating Sequence
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18562670]
Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to adjacent ORF, IRC18/YJL037W, and the irc18 loh1 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants; SWAT-GFP and mCherry fusion proteins localize to the cytosol; proposed role in maintenance of genome integrity
ARS1013; Autonomously Replicating Sequence; inefficient as chromosomal replication origin; replication capacity dependent upon Sum1p
Protein involved in outer spore wall assembly; possible role in assembly of the dityrosine layer; similar to adjacent ORF, LOH1; irc18 loh1 double mutant exhibits reduced dityrosine fluorescence relative to single mutants; SWAT-GFP fusion protein localizes to the ER and vacuole, while mCherry fusion localizes to the vacuole; expression induced in respiratory-deficient cells and carbon-limited chemostat culture; null mutant displays increased levels of spontaneous Rad52p foci
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:12048214|PMID:18497569]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:17420293]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12048214|PMID:26928762]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:12554655|PMID:11557775]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:33678121]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:12554655]
EXISTENCE:genetic interaction:GO:0036010 protein localization to endosome [PMID:29511122]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12554655]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:12048214|PMID:12554655]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:32074001]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:12554655]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:physical interaction:GO:0034498 early endosome to Golgi transport [PMID:12554655]
Sorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:10550050]
EXISTENCE:direct assay:GO:0008251 tRNA-specific adenosine deaminase activity [PMID:10550050]
Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:2661018]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:1325440]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:10367885]
EXISTENCE:direct assay:GO:0034099 luminal surveillance complex [PMID:16873065]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:23704572]
EXISTENCE:genetic interaction:GO:0070880 fungal-type cell wall beta-glucan biosynthetic process [PMID:9430631]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:7568189]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:12808051]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7568189]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:12808051]
EXISTENCE:mutant phenotype:GO:0070972 protein localization to endoplasmic reticulum [PMID:1996357]
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:16209945]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:23153376]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:18975973|PMID:23153376]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:25535329]
EXISTENCE:direct assay:GO:0034512 box C/D RNA binding [PMID:25535329]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:16209945]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:16209945]
EXISTENCE:mutant phenotype:GO:0032040 small-subunit processome [PMID:24357410]
DEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
EXISTENCE:direct assay:GO:0004663 Rab geranylgeranyltransferase activity [PMID:8756702]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27663510]
EXISTENCE:direct assay:GO:0005968 Rab-protein geranylgeranyltransferase complex [PMID:8756702]
EXISTENCE:direct assay:GO:0018344 protein geranylgeranylation [PMID:8756702]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:8232541]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8232541]
EXISTENCE:mutant phenotype:GO:0018344 protein geranylgeranylation [PMID:8232541]
EXISTENCE:physical interaction:GO:0005968 Rab-protein geranylgeranyltransferase complex [PMID:8232542]
Alpha subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:27170178]
EXISTENCE:direct assay:GO:0033597 mitotic checkpoint complex [PMID:11726501]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12473689]
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:22940250]
EXISTENCE:genetic interaction:GO:0044774 mitotic DNA integrity checkpoint signaling [PMID:16651657]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:10704439]
EXISTENCE:mutant phenotype:GO:0051312 chromosome decondensation [PMID:17151360]
EXISTENCE:physical interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:9461437]
EXISTENCE:physical interaction:GO:0033597 mitotic checkpoint complex [PMID:10704439|PMID:11726501]
Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:10637310]
EXISTENCE:direct assay:GO:0042147 retrograde transport, endosome to Golgi [PMID:12686613]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:10637310]
EXISTENCE:mutant phenotype:GO:0090156 cellular sphingolipid homeostasis [PMID:26357016]
EXISTENCE:physical interaction:GO:0000938 GARP complex [PMID:10637310]
Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2)
Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis
hypothetical protein; may interact with ribosomes, based on co-purification experiments
ribonucleotide-diphosphate reductase subunit RNR2
EXISTENCE:direct assay:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:10535923|PMID:10716984]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12732713]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12732713|PMID:9315670]
EXISTENCE:direct assay:GO:0005971 ribonucleoside-diphosphate reductase complex [PMID:10716984]
EXISTENCE:direct assay:GO:0009263 deoxyribonucleotide biosynthetic process [PMID:10535923|PMID:5459124]
Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0001164 RNA polymerase I core promoter sequence-specific DNA binding []
EXISTENCE:direct assay:GO:0001164 RNA polymerase I core promoter sequence-specific DNA binding [PMID:18451108]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:8702872]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7958901]
EXISTENCE:direct assay:GO:0070860 RNA polymerase I core factor complex [PMID:7958901|PMID:8702872|PMID:8887672]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7958901]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:8702872]
Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17895371]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:9335339]
EXISTENCE:mutant phenotype:GO:0030123 AP-3 adaptor complex [PMID:9250663]
Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0099617 matrix side of mitochondrial inner membrane [PMID:30759361]
EXISTENCE:mutant phenotype:GO:0097745 mitochondrial tRNA 5'-end processing [PMID:30759361]
hypothetical protein; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; localizes to the matrix side of the inner mitochondrial membrane
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:11743162|PMID:16824951]
EXISTENCE:direct assay:GO:0032032 myosin I tail binding [PMID:11901111]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:12814545]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:11901111]
EXISTENCE:physical interaction:GO:0032032 myosin I tail binding [PMID:11901111]
Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:17495028]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:17495028]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:16923827]
EXISTENCE:direct assay:GO:0005825 half bridge of spindle pole body [PMID:12486115]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12493774|PMID:12486115]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:18039933|PMID:26928762]
EXISTENCE:genetic interaction:GO:0007129 homologous chromosome pairing at meiosis [PMID:17495028]
EXISTENCE:genetic interaction:GO:0045141 meiotic telomere clustering [PMID:17495028]
EXISTENCE:mutant phenotype:GO:0000741 karyogamy [PMID:16923827]
EXISTENCE:mutant phenotype:GO:0000743 nuclear migration involved in conjugation with cellular fusion [PMID:12493774]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:15355977]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:17495028]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:12486115|PMID:16923827]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:22593213]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:22593213]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:18039933|PMID:22593213]
EXISTENCE:mutant phenotype:GO:0045141 meiotic telomere clustering [PMID:17495028]
Nuclear envelope protein; required for SPB insertion, SPB duplication, Kar5p localization near the SPB and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology)
hypothetical protein; GFP-fusion protein localizes to the cytoplasm; contains two adjacent PH-like domains; conserved in closely related Saccharomyces species
chaperonin-containing T-complex subunit CCT3
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; capable of binding Q/N rich proteins and mediating their folding
EXISTENCE:mutant phenotype:GO:0032837 distributive segregation [PMID:15951820]
EXISTENCE:mutant phenotype:GO:0033597 mitotic checkpoint complex [PMID:10704439]
Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000822 inositol hexakisphosphate binding [PMID:27080106]
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:17079729]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:12584253]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17079729]
EXISTENCE:direct assay:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:direct assay:GO:0008976 polyphosphate kinase activity [PMID:19390046]
EXISTENCE:direct assay:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:11102525|PMID:19390046]
EXISTENCE:mutant phenotype:GO:0006799 polyphosphate biosynthetic process [PMID:27252384|PMID:27080106]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:11823419|PMID:27252384]
EXISTENCE:mutant phenotype:GO:0048016 inositol phosphate-mediated signaling [PMID:27080106]
EXISTENCE:physical interaction:GO:0033254 vacuolar transporter chaperone complex [PMID:11823419]
Vacuolar membrane polyphosphate polymerase; subunit of vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
DNA-directed RNA polymerase III subunit RPC17
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:10611227]
EXISTENCE:physical interaction:GO:0006384 transcription initiation at RNA polymerase III promoter [PMID:10611227]
RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis
ARS1014; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:17956976]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:17956976|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:17956976]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:17956976]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:17956976]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17956976]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17956976]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17956976]
Essential subunit of U3-containing 90S preribosome; involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
chaperonin-containing T-complex subunit CCT8
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15520468]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15520468]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:11118453]
EXISTENCE:direct assay:GO:0032786 positive regulation of DNA-templated transcription, elongation [PMID:9110987]
EXISTENCE:direct assay:GO:0070692 CTDK-1 complex [PMID:7565723]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:11118453]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15520468]
EXISTENCE:physical interaction:GO:0045903 positive regulation of translational fidelity [PMID:17545469]
Beta subunit of C-terminal domain kinase I (CTDK-I); which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; relocalizes to the cytosol in response to hypoxia
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations
EXISTENCE:direct assay:GO:0004016 adenylate cyclase activity [PMID:2934138]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22575457|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:22575457]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2196438]
EXISTENCE:genetic interaction:GO:0007188 adenylate cyclase-modulating G protein-coupled receptor signaling pathway [PMID:2934138]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:2934138]
EXISTENCE:mutant phenotype:GO:0004016 adenylate cyclase activity [PMID:6360999]
Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; scaffold protein for Ras2-Ira interaction; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:11726510]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:15077113]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:8923208]
EXISTENCE:mutant phenotype:GO:0034067 protein localization to Golgi apparatus [PMID:15077113|PMID:15077114]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:16926193]
Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12446688]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:12446688]
Mitochondrial inner membrane protein; required for assembly of cytochrome c oxidase
dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:29301962]
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:9015380]
EXISTENCE:direct assay:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:29301962]
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084]
EXISTENCE:direct assay:GO:0018279 protein N-linked glycosylation via asparagine [PMID:9988747]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Alpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:9087403]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:mutant phenotype:GO:0004175 endopeptidase activity [PMID:9207060|PMID:9312134]
Beta 1 subunit of the 20S proteasome; responsible for cleavage after acidic residues in peptides
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11274162|PMID:27246536]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0003333 amino acid transmembrane transport [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0005302 L-tyrosine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015186 L-glutamine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015188 L-isoleucine transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015824 proline transport [PMID:27246536]
Vacuolar transporter; imports large neutral amino acids into vacuole; targeted to vacuole via AP-3 pathway; member of family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11914276]
EXISTENCE:direct assay:GO:0034457 Mpp10 complex [PMID:17515605]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9315638]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9315638]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9315638]
EXISTENCE:mutant phenotype:GO:0032040 small-subunit processome [PMID:12242301]
Component of the SSU processome and 90S preribosome; required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis
ARS1015; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0000752 agglutination involved in conjugation with cellular fusion [PMID:2673555|PMID:2677666]
EXISTENCE:mutant phenotype:GO:0050839 cell adhesion molecule binding [PMID:11369849|PMID:2673555|PMID:2677666]
Alpha-agglutinin of alpha-cells; binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
EXISTENCE:curator inference:GO:0016192 vesicle-mediated transport [PMID:22190733]
EXISTENCE:direct assay:GO:0030122 AP-2 adaptor complex [PMID:10564262]
EXISTENCE:direct assay:GO:0030139 endocytic vesicle [PMID:22190733]
Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26450372]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26450372]
EXISTENCE:mutant phenotype:GO:0055072 iron ion homeostasis [PMID:26450372]
Protein with a potential role in response to iron deprivation; transcription increases during iron deprivation and during treatment with 2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and copper; regulated by Aft1p and, to a lesser extent, by Aft2p; originally identified as a syntenic homolog of an Ashbya gossypii gene; localizes to nucleus and cytoplasm, and nuclear localization is enhanced under iron-replete conditions
DNA-directed DNA polymerase delta subunit POL31
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:18635534]
EXISTENCE:direct assay:GO:0006278 RNA-templated DNA biosynthetic process [PMID:17429354]
EXISTENCE:direct assay:GO:0016035 zeta DNA polymerase complex [PMID:23066099|PMID:22711820]
EXISTENCE:direct assay:GO:0043137 DNA replication, removal of RNA primer [PMID:16837458]
Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia
translation initiation factor eIF2 subunit alpha
EXISTENCE:direct assay:GO:0001731 formation of translation preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14698289|PMID:16246727]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:11018020|PMID:15838098]
EXISTENCE:direct assay:GO:1990856 methionyl-initiator methionine tRNA binding [PMID:16565414]
EXISTENCE:mutant phenotype:GO:0005850 eukaryotic translation initiation factor 2 complex [PMID:2649894]
Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22412880|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22412880|PMID:14562095]
hypothetical protein; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH2
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:27184763]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17726063]
EXISTENCE:mutant phenotype:GO:0072593 reactive oxygen species metabolic process [PMID:17726063]
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication
ARS1016; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004781 sulfate adenylyltransferase (ATP) activity [PMID:10092498]
EXISTENCE:mutant phenotype:GO:0000096 sulfur amino acid metabolic process [PMID:1101032]
EXISTENCE:mutant phenotype:GO:0004781 sulfate adenylyltransferase (ATP) activity [PMID:197388]
EXISTENCE:mutant phenotype:GO:0019379 sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) [PMID:197388]
ATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
EXISTENCE:direct assay:GO:0005787 signal peptidase complex [PMID:1846444|PMID:8910564]
EXISTENCE:genetic interaction:GO:0006465 signal peptide processing [PMID:8663399]
EXISTENCE:genetic interaction:GO:0045047 protein targeting to ER [PMID:8663399]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:8663399]
EXISTENCE:physical interaction:GO:0045047 protein targeting to ER [PMID:8663399]
Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
Accessory component of the Ccr4-NOT complex; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
ARS1017; Autonomously Replicating Sequence
hypothetical protein; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; null mutant is viable
glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I
EXISTENCE:direct assay:GO:0000030 mannosyltransferase activity [PMID:15635094]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:16134120]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:16134120]
EXISTENCE:physical interaction:GO:0006506 GPI anchor biosynthetic process [PMID:15635094]
EXISTENCE:physical interaction:GO:1990529 glycosylphosphatidylinositol-mannosyltransferase I complex [PMID:15635094]
Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I; involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
EXISTENCE:mutant phenotype:GO:0032790 ribosome disassembly [PMID:34016977]
hypothetical protein; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
hypothetical protein; localizes to endoplasmic reticulum and cytoplasm; null mutant exhibits abnormal lipid metabolic phenotype; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0004160 dihydroxy-acid dehydratase activity [PMID:14124940]
EXISTENCE:mutant phenotype:GO:0009082 branched-chain amino acid biosynthetic process [PMID:14124940]
Dihydroxyacid dehydratase; putative 2Fe-2S protein; catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:15728580|PMID:9867817]
EXISTENCE:direct assay:GO:0035307 positive regulation of protein dephosphorylation [PMID:11904169]
EXISTENCE:direct assay:GO:1901407 regulation of phosphorylation of RNA polymerase II C-terminal domain [PMID:22778132]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:14704159]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:19854134]
EXISTENCE:genetic interaction:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15166139]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10899126]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:14704159]
EXISTENCE:mutant phenotype:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:15728580]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:19854134|PMID:14704159|PMID:22778132]
EXISTENCE:mutant phenotype:GO:0031064 negative regulation of histone deacetylation [PMID:10899127]
EXISTENCE:mutant phenotype:GO:0035307 positive regulation of protein dephosphorylation [PMID:19854134]
EXISTENCE:mutant phenotype:GO:2000059 negative regulation of ubiquitin-dependent protein catabolic process [PMID:19597489]
EXISTENCE:mutant phenotype:GO:2000749 positive regulation of rDNA heterochromatin formation [PMID:10899127]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:10531363|PMID:10899126]
EXISTENCE:physical interaction:GO:0031064 negative regulation of histone deacetylation [PMID:10899127]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10899126]
Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated S/T residues N-terminal to proline; regulates phosphorylation of RNAPII large subunit (Rpo21p) C-terminal domain (CTD) at Ser7; associates with phospho-Ser5 form of RNAPII in vivo; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription, trimethylation of histone H3; human ortholog PIN1 can complement yeast null and ts mutants
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:10092594]
EXISTENCE:direct assay:GO:0047617 acyl-CoA hydrolase activity [PMID:10092594]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:19830908]
EXISTENCE:mutant phenotype:GO:0019395 fatty acid oxidation [PMID:10092594]
Peroxisomal acyl-CoA thioesterase; likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16783010]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:8536989]
Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10369684|PMID:15075370]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12730603]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:10369684|PMID:11720284]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:18029398]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10428970]
Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
methylthioribulose 1-phosphate dehydratase MDE1
EXISTENCE:mutant phenotype:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:18625006]
EXISTENCE:mutant phenotype:GO:0046570 methylthioribulose 1-phosphate dehydratase activity [PMID:18625006]
5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11901108]
EXISTENCE:genetic interaction:GO:0034354 'de novo' NAD biosynthetic process from tryptophan [PMID:12062417]
EXISTENCE:mutant phenotype:GO:0000334 3-hydroxyanthranilate 3,4-dioxygenase activity [PMID:9539135]
3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YJRWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
YJRWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:mutant phenotype:GO:0019320 hexose catabolic process [PMID:23185390]
hypothetical protein; expression repressed in carbon limited vs carbon replete chemostat cultures; non-essential gene; contains a PH-like domain; RBH2 has a paralog, RBH1, that arose from the whole genome duplication
Arf family guanine nucleotide exchange factor GEA1
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:11294905]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10430582]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11294905]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:14668359]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11493664|PMID:8945477]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11493664]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20444982]
Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:10942767]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:10942767]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10942767]
EXISTENCE:physical interaction:GO:0005829 cytosol [PMID:10942767]
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11283612]
EXISTENCE:genetic interaction:GO:0007035 vacuolar acidification [PMID:11283612|PMID:11844802]
EXISTENCE:genetic interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:11844802]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:11283612|PMID:11844802]
EXISTENCE:mutant phenotype:GO:0043254 regulation of protein-containing complex assembly [PMID:11283612|PMID:11844802]
EXISTENCE:mutant phenotype:GO:0045022 early endosome to late endosome transport [PMID:15090613]
EXISTENCE:physical interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:11283612]
EXISTENCE:physical interaction:GO:0043291 RAVE complex [PMID:11283612|PMID:11844802]
Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:18503002]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:18503002|PMID:8381337]
Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:8702468]
EXISTENCE:genetic interaction:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17023424]
EXISTENCE:genetic interaction:GO:0006289 nucleotide-excision repair [PMID:17023424]
EXISTENCE:mutant phenotype:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:7957102|PMID:32690696]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:23991048]
Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
putative E3 ubiquitin-protein ligase HUL4
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:15828860]
Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex
Mitochondrial hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:17662057]
EXISTENCE:direct assay:GO:0005247 voltage-gated chloride channel activity [PMID:12074596]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15710404]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17662057]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15710404]
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:9614122]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17662057]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:9520490|PMID:9614122]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9520490]
Voltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15226434|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0016072 rRNA metabolic process [PMID:15242642]
EXISTENCE:physical interaction:GO:0042254 ribosome biogenesis [PMID:15226434]
Protein required for normal metabolism of the rRNA primary transcript; nucleolar protein; proposed to be involved in ribosome biogenesis
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:11823431|PMID:18046406]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:8816998|PMID:8565072]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:22331846]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:8816998|PMID:8565072]
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
EXISTENCE:direct assay:GO:0016035 zeta DNA polymerase complex [PMID:22711820|PMID:23066099]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:17671506]
EXISTENCE:mutant phenotype:GO:0006277 DNA amplification [PMID:23271978]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:17681555]
Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p
EXISTENCE:curator inference:GO:0006413 translational initiation [PMID:9023104]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0071074 eukaryotic initiation factor eIF2 binding [PMID:14698289]
Methionine initiator tRNA (tRNA-Met); predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:12006663]
EXISTENCE:direct assay:GO:0034424 Vps55/Vps68 complex [PMID:18216282]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:18216282]
EXISTENCE:physical interaction:GO:0034424 Vps55/Vps68 complex [PMID:18216282]
Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:14517234|PMID:14638855]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:24769239|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7935837]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:11096111]
EXISTENCE:direct assay:GO:0030150 protein import into mitochondrial matrix [PMID:8654364]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:10464305]
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:10464305]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9973563]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:10779357]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:16460754]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:8408192]
Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11836525]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11836525]
EXISTENCE:direct assay:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:11967159]
EXISTENCE:mutant phenotype:GO:0005656 nuclear pre-replicative complex [PMID:11967159]
DNA replication licensing factor; required for pre-replication complex assembly
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:27196944]
EXISTENCE:genetic interaction:GO:0006452 translational frameshifting [PMID:19424157]
EXISTENCE:genetic interaction:GO:0045901 positive regulation of translational elongation [PMID:19424157]
Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:9866716]
EXISTENCE:direct assay:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:7851399]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:7851399]
EXISTENCE:direct assay:GO:0009055 electron transfer activity [PMID:18975895|PMID:7851399]
EXISTENCE:direct assay:GO:1901612 cardiolipin binding [PMID:30182710]
Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication; human homolog CYC1 can complement yeast null mutant; mutations in human CYC1 cause insulin-responsive hyperglycemia
EXISTENCE:direct assay:GO:0003951 NAD+ kinase activity [PMID:11425472]
EXISTENCE:direct assay:GO:0006741 NADP biosynthetic process [PMID:11425472|PMID:15978040]
EXISTENCE:direct assay:GO:0042736 NADH kinase activity [PMID:15978040]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:8662826]
ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:10094305]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:11018040]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:10094305|PMID:11018040]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:10094305]
EXISTENCE:direct assay:GO:0071014 post-mRNA release spliceosomal complex [PMID:10094305]
EXISTENCE:direct assay:GO:0071020 post-spliceosomal complex [PMID:10094305]
EXISTENCE:genetic interaction:GO:0000384 first spliceosomal transesterification activity [PMID:16103217]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:10094305]
EXISTENCE:mutant phenotype:GO:0000389 mRNA 3'-splice site recognition [PMID:16103217]
Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:25378625]
EXISTENCE:direct assay:GO:0016156 fumarate reductase (NADH) activity [PMID:9587404]
EXISTENCE:mutant phenotype:GO:0034975 protein folding in endoplasmic reticulum [PMID:25378625]
EXISTENCE:mutant phenotype:GO:0046443 FAD metabolic process [PMID:17345583]
Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; acts as electron acceptor in mitochondrial intermembrane space; has two translation start sites, one at the annotated start codon which produces an ER-targeted form required for anaerobic growth, and one at codon 32 which produces a mitochondrially-targeted form; OSM1 has a paralog, FRD1, that arose from the whole genome duplication
Glycine tRNA (tRNA-Gly); can mutate to suppress +1 frameshift mutations
EXISTENCE:direct assay:GO:0000113 nucleotide-excision repair factor 4 complex [PMID:9497356]
EXISTENCE:direct assay:GO:0000715 nucleotide-excision repair, DNA damage recognition [PMID:15177043]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:9497356]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:16675952]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:9497356]
EXISTENCE:direct assay:GO:0008104 protein localization [PMID:15226437]
EXISTENCE:direct assay:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:16675952]
EXISTENCE:mutant phenotype:GO:0000715 nucleotide-excision repair, DNA damage recognition [PMID:15177043]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:16675952]
Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
EXISTENCE:direct assay:GO:0005092 GDP-dissociation inhibitor activity [PMID:19948498]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12048186|PMID:16682821|PMID:18667533]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10220406|PMID:15147270]
EXISTENCE:direct assay:GO:1990334 Bfa1-Bub2 complex [PMID:25658911]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:15147270|PMID:18667533]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:16039591]
EXISTENCE:physical interaction:GO:1990334 Bfa1-Bub2 complex [PMID:11493673]
Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23204190]
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:23204190]
EXISTENCE:direct assay:GO:0015079 potassium ion transmembrane transporter activity [PMID:23204190]
EXISTENCE:direct assay:GO:0071805 potassium ion transmembrane transport [PMID:23204190]
Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; localized to sites of polarized growth; member of a fungal-specific gene family; potential Cdc28p substrate; KCH1 has a paralog, PRM6, that arose from the whole genome duplication
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; low abundance tRNA required for growth on nonfermentable carbon sources at high temperatures, for synthesis of heat shock protein Ssc1p, and for Ty1 retrotransposition through the regulation of translational frameshifting
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25170085]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:25170085]
Protein involved in C/D snoRNP assembly; regulates abundance of Rsa1p; required for growth at high temperature; mutations in human homolog ZNHIT3 cause PEHO syndrome
ARS1018; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
bifunctional thymidylate/uridylate kinase
EXISTENCE:direct assay:GO:0004550 nucleoside diphosphate kinase activity [PMID:19540237]
EXISTENCE:direct assay:GO:0004798 thymidylate kinase activity [PMID:6088527|PMID:6094555|PMID:19540237]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:6094555]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:6094555]
EXISTENCE:direct assay:GO:0006227 dUDP biosynthetic process [PMID:6094555]
EXISTENCE:direct assay:GO:0006233 dTDP biosynthetic process [PMID:6094555|PMID:6088527|PMID:19540237]
EXISTENCE:direct assay:GO:0006235 dTTP biosynthetic process [PMID:19540237]
EXISTENCE:direct assay:GO:0009041 uridylate kinase activity [PMID:6094555]
EXISTENCE:genetic interaction:GO:0004798 thymidylate kinase activity [PMID:6091111]
EXISTENCE:genetic interaction:GO:0006233 dTDP biosynthetic process [PMID:6091111]
EXISTENCE:mutant phenotype:GO:0004550 nucleoside diphosphate kinase activity [PMID:19540237]
EXISTENCE:mutant phenotype:GO:0004798 thymidylate kinase activity [PMID:19540237|PMID:6088527|PMID:6091111]
EXISTENCE:mutant phenotype:GO:0006233 dTDP biosynthetic process [PMID:19540237|PMID:6088527|PMID:6091111]
EXISTENCE:mutant phenotype:GO:0006235 dTTP biosynthetic process [PMID:19540237]
Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant
EXISTENCE:curator inference:GO:0006886 intracellular protein transport [PMID:10564262]
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:8157009]
EXISTENCE:physical interaction:GO:0030122 AP-2 adaptor complex [PMID:10564262]
Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894|PMID:16510118]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12206772]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16510118]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:12518319]
EXISTENCE:mutant phenotype:GO:0000296 spermine transport [PMID:8920934]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:11003661]
EXISTENCE:mutant phenotype:GO:0015847 putrescine transport [PMID:9154797]
EXISTENCE:mutant phenotype:GO:0015848 spermidine transport [PMID:9154797]
Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:9407032]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7891681|PMID:8665859|PMID:9894911]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:15302821]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:7891681]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:15111622]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:11222754|PMID:2057354|PMID:2249662|PMID:9407032]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12820973]
EXISTENCE:direct assay:GO:0089713 Cbf1-Met4-Met28 complex [PMID:8665859|PMID:9171357]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:9584087]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10921921|PMID:22696683|PMID:15302821]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:21131275|PMID:2249662|PMID:7891681]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:7891681]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:2057354|PMID:2185892|PMID:2188087]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22696683|PMID:7601277|PMID:7891681|PMID:17351075|PMI D:20935143]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:20935143]
EXISTENCE:mutant phenotype:GO:0098781 ncRNA transcription [PMID:31391265]
EXISTENCE:physical interaction:GO:0000776 kinetochore [PMID:11070082]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:8665859]
Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0006491 N-glycan processing [PMID:28101612]
Protein required for N-glycan mannosylphosphorylation; non-essential gene; transcription repressed by Rm101p; MNN14 has a paralog, MNN4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008418 protein-N-terminal asparagine amidohydrolase activity [PMID:23218487|PMID:7744855]
EXISTENCE:direct assay:GO:0070773 protein-N-terminal glutamine amidohydrolase activity [PMID:23218487]
EXISTENCE:mutant phenotype:GO:0008418 protein-N-terminal asparagine amidohydrolase activity [PMID:7744855]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:7744855]
EXISTENCE:mutant phenotype:GO:0031365 N-terminal protein amino acid modification [PMID:7744855]
EXISTENCE:mutant phenotype:GO:0070773 protein-N-terminal glutamine amidohydrolase activity [PMID:7744855]
Amidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation
DNA-directed RNA polymerase I core subunit RPA12
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9837969|PMID:8417319]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:1871118|PMID:9121426]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27637775]
EXISTENCE:mutant phenotype:GO:0006363 termination of RNA polymerase I transcription [PMID:15073335|PMID:18413718]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:27637775]
RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex; physically interacts with transcriptional activator Msn4p, to regulate transcription of AYR1, a gene involved in lipid metabolism
chaperonin-containing T-complex subunit CCT5
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:15657399]
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:9210376]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:15657399]
EXISTENCE:mutant phenotype:GO:0034314 Arp2/3 complex-mediated actin nucleation [PMID:16862144]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:15534003|PMID:10377407]
Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
phosphatidylinositol kinase-related protein kinase TOR1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12719473]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:18723607|PMID:19748353|PMID:25046117|PMID:26928762|P MID:12719473]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:26582391]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16900101]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16900101|PMID:18723607]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10973982]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:12719473]
EXISTENCE:direct assay:GO:0018105 peptidyl-serine phosphorylation [PMID:26582391]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:9461583]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:27922823]
EXISTENCE:genetic interaction:GO:0034605 cellular response to heat [PMID:27922823]
EXISTENCE:genetic interaction:GO:1905356 regulation of snRNA pseudouridine synthesis [PMID:27268497]
EXISTENCE:mutant phenotype:GO:0001558 regulation of cell growth [PMID:12171921]
EXISTENCE:mutant phenotype:GO:0004672 protein kinase activity [PMID:10329624]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:8741837]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:17698581]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:14736892]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:12719473|PMID:8186460]
EXISTENCE:mutant phenotype:GO:0031930 mitochondria-nucleus signaling pathway [PMID:11997479]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:31144305]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:10198052]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:16900101]
EXISTENCE:mutant phenotype:GO:0044387 negative regulation of protein kinase activity by regulation of protein phosphorylation [PMID:16980405]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:9096347]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:8741837]
EXISTENCE:mutant phenotype:GO:0090153 regulation of sphingolipid biosynthetic process [PMID:23363605]
EXISTENCE:physical interaction:GO:0031931 TORC1 complex [PMID:12408816]
PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; indole-3-acetic acid, an ATP-competitive inhibitor of TORC1, accumulates upon entry into stationary phase; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:26182403]
EXISTENCE:direct assay:GO:0062092 Yae1-Lto1 complex [PMID:26182403]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:26182403]
Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human YAE1D1, which is overexpressed in oral cancers; co-expression of YAE1D1 and LTO1 homolog ORAOV1 restores growth and Rli1p maturation after YAE1 depletion
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:12604797|PMID:8995429]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23613772]
EXISTENCE:direct assay:GO:0005663 DNA replication factor C complex [PMID:8202350]
EXISTENCE:direct assay:GO:0006272 leading strand elongation [PMID:1346062]
EXISTENCE:direct assay:GO:0031389 Rad17 RFC-like complex [PMID:12604797|PMID:10660302]
EXISTENCE:mutant phenotype:GO:0000076 DNA replication checkpoint signaling [PMID:9671499]
EXISTENCE:physical interaction:GO:0007062 sister chromatid cohesion [PMID:11389843]
EXISTENCE:physical interaction:GO:0031389 Rad17 RFC-like complex [PMID:10660302]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
EXISTENCE:physical interaction:GO:0031391 Elg1 RFC-like complex [PMID:12912927]
Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
nucleoside triphosphate pyrophosphohydrolase HAM1
EXISTENCE:direct assay:GO:0004170 dUTP diphosphatase activity [PMID:17899088]
EXISTENCE:direct assay:GO:0008828 dATP diphosphatase activity [PMID:22531138]
EXISTENCE:direct assay:GO:0009213 pyrimidine deoxyribonucleoside triphosphate catabolic process [PMID:17899088|PMID:22531138]
EXISTENCE:direct assay:GO:0009217 purine deoxyribonucleoside triphosphate catabolic process [PMID:17090528|PMID:17899088|PMID:22531138]
EXISTENCE:direct assay:GO:0035870 dITP diphosphatase activity [PMID:17090528|PMID:22531138]
EXISTENCE:direct assay:GO:0036217 dGTP diphosphatase activity [PMID:17090528|PMID:22531138]
EXISTENCE:direct assay:GO:0036218 dTTP diphosphatase activity [PMID:22531138]
EXISTENCE:direct assay:GO:0036219 GTP diphosphatase activity [PMID:22531138]
EXISTENCE:direct assay:GO:0036220 ITP diphosphatase activity [PMID:17899088|PMID:22531138]
EXISTENCE:direct assay:GO:0036221 UTP diphosphatase activity [PMID:17899088]
EXISTENCE:direct assay:GO:0036222 XTP diphosphatase activity [PMID:22531138]
EXISTENCE:direct assay:GO:0047840 dCTP diphosphatase activity [PMID:22531138]
EXISTENCE:mutant phenotype:GO:0009213 pyrimidine deoxyribonucleoside triphosphate catabolic process [PMID:17899088]
EXISTENCE:mutant phenotype:GO:0009217 purine deoxyribonucleoside triphosphate catabolic process [PMID:22308425]
Nucleoside triphosphate pyrophosphohydrolase; active against various substrates including ITP, dITP and XTP; mediates exclusion of non canonical purines, pyrimidines from dNTP pools; functions with YJL055W to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA without affecting uptake or incorporation of uracil into RNA; protein abundance increases in response to DNA replication stress; yeast HAM1 can complement knockdown of human homolog ITPA
EXISTENCE:direct assay:GO:0008612 peptidyl-lysine modification to peptidyl-hypusine [PMID:16371467]
EXISTENCE:direct assay:GO:0019135 deoxyhypusine monooxygenase activity [PMID:16371467]
EXISTENCE:mutant phenotype:GO:0000226 microtubule cytoskeleton organization [PMID:14767070]
EXISTENCE:mutant phenotype:GO:0008612 peptidyl-lysine modification to peptidyl-hypusine [PMID:16371467]
EXISTENCE:mutant phenotype:GO:0019135 deoxyhypusine monooxygenase activity [PMID:16371467]
Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:21844196]
EXISTENCE:direct assay:GO:0006913 nucleocytoplasmic transport [PMID:21844196]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:15082539]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:21844196]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:21532343]
Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
EXISTENCE:direct assay:GO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity [PMID:2445736]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:2445736]
EXISTENCE:direct assay:GO:0080101 phosphatidyl-N-dimethylethanolamine N-methyltransferase activity [PMID:2445736]
EXISTENCE:genetic interaction:GO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity [PMID:2445736]
EXISTENCE:genetic interaction:GO:0006656 phosphatidylcholine biosynthetic process [PMID:2445736]
EXISTENCE:genetic interaction:GO:0080101 phosphatidyl-N-dimethylethanolamine N-methyltransferase activity [PMID:2445736]
EXISTENCE:mutant phenotype:GO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity [PMID:2684666]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:6337128|PMID:23000174]
EXISTENCE:mutant phenotype:GO:0080101 phosphatidyl-N-dimethylethanolamine N-methyltransferase activity [PMID:2684666]
Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9860978]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:11509570|PMID:9860978]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:9860978|PMID:11024003]
Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:10037752]
EXISTENCE:direct assay:GO:0000136 mannan polymerase complex [PMID:10037752]
Alpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
EXISTENCE:curator inference:GO:0030011 maintenance of cell polarity [PMID:10882120]
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:1934633]
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:8791410]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:18550837|PMID:22144691|PMID:29321251]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:12665577]
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:8636217]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18826657|PMID:24390141]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:18826657]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:18826657]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18550837]
EXISTENCE:direct assay:GO:0032160 septin filament array [PMID:15282341]
EXISTENCE:direct assay:GO:0045860 positive regulation of protein kinase activity [PMID:14527412]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0072687 meiotic spindle [PMID:18826657]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:25971666]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:4950437]
EXISTENCE:mutant phenotype:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:12665577]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:12665577]
EXISTENCE:physical interaction:GO:0031105 septin complex [PMID:9813094]
Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
EXISTENCE:direct assay:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:9099701]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8245029]
EXISTENCE:direct assay:GO:0035435 phosphate ion transmembrane transport [PMID:9099701]
Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
EXISTENCE:curator inference:GO:0019441 tryptophan catabolic process to kynurenine [PMID:21170645]
EXISTENCE:direct assay:GO:0033754 indoleamine 2,3-dioxygenase activity [PMID:21170645]
Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase; required for de novo biosynthesis of NAD+ from tryptophan via kynurenine; up-regulated by telomere uncapping, and interacts genetically with capping gene CDC13; overexpression reduces lipid droplet accumulation by skewing glycolytic flux towards biosynthesis of shikimate, aromatic amino acids, and NAD+ during aging and extends lifespan; regulated by Hst1p and Aftp
hypothetical protein; mutation results in impaired mitochondrial respiration
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:24714493]
EXISTENCE:mutant phenotype:GO:0007007 inner mitochondrial membrane organization [PMID:24714493]
Protein with a role in determining mitochondrial architecture; inner membrane protein that interacts physically and genetically with the MICOS complex and is required for its integrity
EXISTENCE:curator inference:GO:0004402 histone acetyltransferase activity [PMID:17157260]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:17157260]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911]
EXISTENCE:direct assay:GO:1990467 NuA3a histone acetyltransferase complex [PMID:25104842]
EXISTENCE:direct assay:GO:1990468 NuA3b histone acetyltransferase complex [PMID:25104842]
EXISTENCE:physical interaction:GO:0016573 histone acetylation [PMID:17157260]
Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
hypothetical protein; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:21149575]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:19061648]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12446563]
Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
Mitochondrial hypothetical protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8995254]
EXISTENCE:direct assay:GO:0005834 heterotrimeric G-protein complex [PMID:8995254]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8995254]
EXISTENCE:direct assay:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8417317|PMID:8995254]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:2107073]
EXISTENCE:genetic interaction:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8417317]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3151178]
EXISTENCE:mutant phenotype:GO:0005834 heterotrimeric G-protein complex [PMID:2105453]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:8649369]
EXISTENCE:physical interaction:GO:0031680 G-protein beta/gamma-subunit complex [PMID:8649369]
EXISTENCE:physical interaction:GO:0031681 G-protein beta-subunit binding [PMID:8417317|PMID:8649369]
G protein gamma subunit; forms dimer with Ste4p to activate mating signaling pathway, heterotrimer with Gpa1p, Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling; N-terminal phosphorylation of Ste18p functions synergistically with Fus3p docking site on Ste5p to restrict Ste5p plasma membrane recruitment and signaling; combinatorial phosphorylation at S3 and S7, by pheromone and pH stress, modulates Ste18p structure, Fus3p activation
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16608876]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16608876]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:16381814]
EXISTENCE:direct assay:GO:0005874 microtubule [PMID:22842922]
EXISTENCE:direct assay:GO:0032133 chromosome passenger complex [PMID:19158380]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:16381814]
EXISTENCE:genetic interaction:GO:0000776 kinetochore [PMID:10557299]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:16381814]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:16608876]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:10557299]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:22908045]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:19528231]
Subunit of chromosomal passenger complex (CPC); CPC is comprised of Ipl1p-Sli15p-Bir1p-Nbl1p and regulates chromosome segregation; required for chromosome bi-orientation and for spindle assembly checkpoint activation upon reduced sister kinetochore tension; relative distribution to shortened microtubules increases upon DNA replication stress; sumoylated in an Mms21p-dependent manner; human survivin homolog
SCF ubiquitin ligase complex subunit GRR1
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:11080155|PMID:15775961]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:10213692]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11080155]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:11259599|PMID:9346238|PMID:9819356]
EXISTENCE:genetic interaction:GO:0000209 protein polyubiquitination [PMID:9499404]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:16093347]
EXISTENCE:mutant phenotype:GO:0000751 mitotic cell cycle G1 arrest in response to pheromone [PMID:15942932]
EXISTENCE:mutant phenotype:GO:0019005 SCF ubiquitin ligase complex [PMID:9736614]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:9819356]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:29212878]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:29212878]
EXISTENCE:mutant phenotype:GO:0071406 cellular response to methylmercury [PMID:17141224]
EXISTENCE:physical interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:10213692]
F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21685478|PMID:15024427|PMID:23222640]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:23222640]
EXISTENCE:direct assay:GO:0032473 cytoplasmic side of mitochondrial outer membrane [PMID:16107558]
EXISTENCE:genetic interaction:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11101532]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:16107558]
EXISTENCE:mutant phenotype:GO:0051654 establishment of mitochondrion localization [PMID:16107558]
Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:22553209]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:22553209]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:23345395]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:27385335|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0005525 GTP binding [PMID:22553209]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:22553209]
EXISTENCE:mutant phenotype:GO:0031106 septin ring organization [PMID:23345395]
EXISTENCE:mutant phenotype:GO:0097271 protein localization to bud neck [PMID:22553209]
Anillin-like protein involved in bud-site selection; required for the axial budding pattern; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; functions as a platform linking the cytokinesis tag septins to the axial landmark through its multiple domains; in vivo substrate of Cdc28p/Clb2p
cleavage polyadenylation factor subunit FIP1
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:genetic interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:21282348]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:11238938]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:21282348]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:7736590]
Subunit of cleavage polyadenylation factor (CPF); interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8462841]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:29472539]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:8205723]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:20876298]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:8205723]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9660952]
EXISTENCE:physical interaction:GO:0008134 transcription factor binding [PMID:8628320]
Master regulator of meiosis that is active only during meiotic events; activates transcription of early meiotic genes through interaction with Ume6p; regulator of meiotic commitment; degraded by the 26S proteasome following phosphorylation by Ime2p; transcription is negatively regulated in cis by the IRT1 long noncoding antisense RNA
Long noncoding RNA that governs mating-type control of gametogenesis; located in the IME1 promoter; in haploids, expression of IME1, the central inducer of gametogenesis, is inhibited in cis by transcription of IRT1, which recruits the Set2p histone methyltransferase and the Set3p histone deacetylase complex to establish repressive chromatin at the IME1 promoter
Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005469 succinate:fumarate antiporter activity [PMID:9395087]
EXISTENCE:direct assay:GO:0015741 fumarate transport [PMID:9395087]
EXISTENCE:direct assay:GO:0015744 succinate transport [PMID:9395087]
Mitochondrial succinate-fumarate transporter; transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization
EXISTENCE:direct assay:GO:0004032 alditol:NADP+ 1-oxidoreductase activity [PMID:11306085|PMID:12271459]
EXISTENCE:direct assay:GO:0004033 aldo-keto reductase (NADP) activity [PMID:17140678]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:17919749]
EXISTENCE:mutant phenotype:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:mutant phenotype:GO:0042843 D-xylose catabolic process [PMID:12271459]
Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0008198 ferrous iron binding [PMID:22367199]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:15485916]
Iron-binding protein; involved in targeting cytoplasmic SRP-independent proteins to the ER; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
ARS1019; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:1429680]
EXISTENCE:curator inference:GO:0010992 ubiquitin recycling [PMID:1429680]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:1429680]
Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:27922823]
EXISTENCE:mutant phenotype:GO:1903147 negative regulation of autophagy of mitochondrion [PMID:27922823]
Mitochondria hypothetical protein; interacts genetically with TOR1 to regulate chronological lifespan, and the response to both heat shock and oxidative stress; involved in maintaining the integrity of the mitochondrial network; negative regulator of mitophagy flux; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant is viable and displays an elevated frequency of mitochondrial genome loss; similar to murine NOR1
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
mitochondrial 37S ribosomal protein RSM26
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:curator inference:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15086794]
EXISTENCE:direct assay:GO:0000814 ESCRT II complex [PMID:12194858]
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:15329733|PMID:15469844]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:11278625]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:15329733|PMID:15469844]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12194858]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome
EXISTENCE:direct assay:GO:0003883 CTP synthase activity [PMID:7559626]
EXISTENCE:direct assay:GO:0006241 CTP biosynthetic process [PMID:7559626]
EXISTENCE:direct assay:GO:0097268 cytoophidium [PMID:20713603]
EXISTENCE:mutant phenotype:GO:0003883 CTP synthase activity [PMID:8121398]
Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004784 superoxide dismutase activity [PMID:3290902]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18977757|PMID:24647101]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:11500508]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11500508]
EXISTENCE:mutant phenotype:GO:0006801 superoxide metabolic process [PMID:3290902]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:8530401]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:11581253]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:20176017]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24647101]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:23332757]
EXISTENCE:mutant phenotype:GO:0051091 positive regulation of DNA-binding transcription factor activity [PMID:18977757]
EXISTENCE:mutant phenotype:GO:1901856 negative regulation of cellular respiration [PMID:23332757]
EXISTENCE:physical interaction:GO:0050821 protein stabilization [PMID:23332757]
Cytosolic copper-zinc superoxide dismutase; detoxifies superoxide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p, enters nucleus under oxidative stress to promote transcription of stress response genes; human ortholog SOD1 implicated in ALS complements a null allele; abundance increases under DNA replication stress and during exposure to boric acid; localization to mitochondrial intermembrane space is modulated by MICOS complex
EXISTENCE:direct assay:GO:0004001 adenosine kinase activity [PMID:11223943]
EXISTENCE:mutant phenotype:GO:0004001 adenosine kinase activity [PMID:11223943]
EXISTENCE:mutant phenotype:GO:0006144 purine nucleobase metabolic process [PMID:11223943]
Adenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:27242146]
EXISTENCE:mutant phenotype:GO:0070316 regulation of G0 to G1 transition [PMID:27242146]
Protein involved in calcium homeostasis and exit from quiescence; required for proper trehalose levels during quiescence; may play a role in cell wall biosynthesis, mutants are hypersensitive to antifungal, Papulacandin B; null mutants have increased plasmid loss; interacts with Pdr5p
EXISTENCE:mutant phenotype:GO:0071470 cellular response to osmotic stress [PMID:26554900]
hypothetical protein; encodes new type of domain, which ab initio modeling suggests is predominantly alpha-helical; nonessential for growth, deletion increases sensitivity to osmostress; expressed at moderate to high abundance; ORF also present in strains EC1118, YJM789, RM11-1a, and AWRI1631; Gln24 in S288C reference is substituted with Arg in wine strain EC1118; predicted S-palmitoylation site on Cys2, suggesting membrane association; transcript previously mischaracterized as SUT646
EXISTENCE:mutant phenotype:GO:0000226 microtubule cytoskeleton organization [PMID:10628851]
hypothetical protein; required for normal microtubule organization
carbamoyl-phosphate synthase (glutamine-hydrolyzing) CPA2
EXISTENCE:direct assay:GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [PMID:8626695]
EXISTENCE:direct assay:GO:0005951 carbamoyl-phosphate synthase complex [PMID:206535]
EXISTENCE:mutant phenotype:GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [PMID:6358221]
EXISTENCE:mutant phenotype:GO:0006526 arginine biosynthetic process [PMID:8626695]
Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:genetic interaction:GO:0046856 phosphatidylinositol dephosphorylation [PMID:15169871]
EXISTENCE:mutant phenotype:GO:0004438 phosphatidylinositol-3-phosphate phosphatase activity [PMID:15169871]
EXISTENCE:mutant phenotype:GO:0046856 phosphatidylinositol dephosphorylation [PMID:15169871]
Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27392156]
Peroxisomal matrix protein with naturally active promoter; well-conserved in fungi; localized to peroxisomes under physiological growth conditions; levels of some amino acids are altered upon both knockout and overexpression, suggesting potential involvement of Pxp2p in amino acid metabolism or related cellular metabolic processes (needs further study); GFP-fusion protein displays inherent dual localization with large proportion localizing to cytosol
EXISTENCE:direct assay:GO:0000444 MIS12/MIND type complex [PMID:14633972]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14657030]
EXISTENCE:direct assay:GO:0007059 chromosome segregation [PMID:12455957]
Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for kinetochore bi-orientation and accurate chromosome segregation; complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; homologous to metazoan CENP-H proteins
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; identified based on homology to Ashbya gossypii
mitochondrial 37S ribosomal protein RSM7
Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein
hypothetical protein; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
Protein distantly similar to ceramide synthases, Lag1p and Lac1p; null mutant is sensitive to expression of the top1-T722A allele; mutation confers sensitivity to copper
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:11581258|PMID:27129777]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11581258]
EXISTENCE:direct assay:GO:0016485 protein processing [PMID:27129777]
EXISTENCE:direct assay:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:11581258]
EXISTENCE:genetic interaction:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:10825201|PMID:11581258|PMID:9065405]
EXISTENCE:genetic interaction:GO:0071586 CAAX-box protein processing [PMID:9065405]
EXISTENCE:genetic interaction:GO:0071712 ER-associated misfolded protein catabolic process [PMID:26771486]
EXISTENCE:mutant phenotype:GO:0005637 nuclear inner membrane [PMID:18923140]
EXISTENCE:mutant phenotype:GO:0071432 peptide mating pheromone maturation involved in positive regulation of conjugation with cellular fusion [PMID:10825201|PMID:9065405]
EXISTENCE:mutant phenotype:GO:0071586 CAAX-box protein processing [PMID:9065405]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:26771486]
EXISTENCE:mutant phenotype:GO:0120236 negative regulation of post-translational protein targeting to membrane, translocation [PMID:32513868]
Highly conserved zinc metalloprotease; component of the ER quality control mechanism that removes faulty proteins clogging translocation channels; inhibits SRP-independent translocation into the ER; has two roles in a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves isoprenylated and non-prenylated oligopeptides; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and complements the null mutant
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:10628851]
hypothetical protein; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
EXISTENCE:direct assay:GO:0032453 histone H3K4 demethylase activity [PMID:17310254]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27325136]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:25248920]
EXISTENCE:mutant phenotype:GO:0032453 histone H3K4 demethylase activity [PMID:17310254|PMID:17369256]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:23123093|PMID:27325136]
EXISTENCE:mutant phenotype:GO:0060623 regulation of chromosome condensation [PMID:25248920]
EXISTENCE:mutant phenotype:GO:0071041 antisense RNA transcript catabolic process [PMID:23123093]
EXISTENCE:mutant phenotype:GO:1902275 regulation of chromatin organization [PMID:27325136]
EXISTENCE:mutant phenotype:GO:1904388 negative regulation of ncRNA transcription associated with protein coding gene TSS/TES [PMID:23123093]
JmjC domain family histone demethylase; promotes global demethylation of H3K4 and represses noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p; phosphorylation by Tpk2p during glycolysis inhibits activity, nuclear localization, chromatin binding and promotes Not4p Ub-mediated degradation; negatively regulated by H3K14 acetylation; regulates sporulation timing by extending period of active transcription; regulates rDNA silencing; human homolog is JARID1C
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:10628851]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:16251356]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:10628851]
hypothetical protein; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core [PMID:15294286|PMID:17082766]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18722382|PMID:15294286]
EXISTENCE:direct assay:GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1) [PMID:15294286]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core [PMID:6225776]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:2529856|PMID:6225776]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:6225776]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:2529856]
Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:18086897]
EXISTENCE:mutant phenotype:GO:0106034 protein maturation by [2Fe-2S] cluster transfer [PMID:29343425]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:18086897|PMID:29343425]
Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:385049|PMID:6814480]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:20419091]
Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347|PMID:26928762]
hypothetical protein; expression induced under calcium shortage
ARS1020; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12967568]
EXISTENCE:direct assay:GO:0030125 clathrin vesicle coat [PMID:12483220]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:12967568]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:14630930]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:14630930]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:20658963]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:12483220]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:12967568]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:15107463]
EXISTENCE:mutant phenotype:GO:0071985 multivesicular body sorting pathway [PMID:12967568]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12483220]
Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
hypothetical protein involved in vacuolar protein sorting; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:16208474]
Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains; partially overlaps the verified ORF RSF2
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689]
EXISTENCE:mutant phenotype:GO:0000454 snoRNA guided rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376|PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748|PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:3327689]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U2129, U2133, and U2264
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:25086354]
EXISTENCE:direct assay:GO:0018023 peptidyl-lysine trimethylation [PMID:25086354]
EXISTENCE:mutant phenotype:GO:0018023 peptidyl-lysine trimethylation [PMID:25086354|PMID:25231983]
S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A
EXISTENCE:mutant phenotype:GO:0003962 cystathionine gamma-synthase activity [PMID:10821189]
EXISTENCE:mutant phenotype:GO:0006790 sulfur compound metabolic process [PMID:10821189]
EXISTENCE:mutant phenotype:GO:0019346 transsulfuration [PMID:10821189]
Cystathionine gamma-synthase, converts cysteine into cystathionine; STR2 has a paralog, YML082W, that arose from the whole genome duplication
mannosyl-oligosaccharide 1,2-alpha-mannosidase
EXISTENCE:direct assay:GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [PMID:8910350]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8864657]
EXISTENCE:mutant phenotype:GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [PMID:1714453|PMID:8439291]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:8905927]
EXISTENCE:mutant phenotype:GO:0035977 protein deglycosylation involved in glycoprotein catabolic process [PMID:9732283]
Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9852143]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9852143|PMID:22842922]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:9852143]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:9755161]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:9755161]
Karyopherin; a carrier protein involved in nuclear import of proteins; importin beta homolog
EXISTENCE:genetic interaction:GO:0004422 hypoxanthine phosphoribosyltransferase activity [PMID:10217799]
EXISTENCE:genetic interaction:GO:0046100 hypoxanthine metabolic process [PMID:10217799]
EXISTENCE:mutant phenotype:GO:0000310 xanthine phosphoribosyltransferase activity [PMID:10217799]
EXISTENCE:mutant phenotype:GO:0032265 XMP salvage [PMID:10217799]
Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine
hypothetical protein; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11782448]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11782448]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
EXISTENCE:direct assay:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:12221072]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10051608|PMID:10469659]
EXISTENCE:direct assay:GO:0140318 protein transporter activity [PMID:10051608|PMID:10469659]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10469659]
EXISTENCE:mutant phenotype:GO:0140318 protein transporter activity [PMID:10469659]
EXISTENCE:physical interaction:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:10051608]
Mitochondrial intermembrane space protein; forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome, also known as deafness-dystonia-optic neuronopathy (DDON) syndrome; human TIMM8A can complement yeast null mutant
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:27172216]
EXISTENCE:mutant phenotype:GO:0031669 cellular response to nutrient levels [PMID:27172216]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:27172216]
EXISTENCE:mutant phenotype:GO:0071472 cellular response to salt stress [PMID:27172216]
Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; involved in the cellular stress response; similar to S. pombe Tti2p; may interact with Rsm23p; GFP-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0004783 sulfite reductase (NADPH) activity [PMID:6751400]
EXISTENCE:direct assay:GO:0009337 sulfite reductase complex (NADPH) [PMID:6751400|PMID:9003798]
EXISTENCE:mutant phenotype:GO:0000097 sulfur amino acid biosynthetic process [PMID:1479340]
EXISTENCE:mutant phenotype:GO:0000103 sulfate assimilation [PMID:19236486]
Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine
ARS1021; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:23716719]
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:23716719]
EXISTENCE:direct assay:GO:1990130 GATOR1 complex [PMID:21900499]
EXISTENCE:genetic interaction:GO:0032007 negative regulation of TOR signaling [PMID:23716719]
EXISTENCE:genetic interaction:GO:0051058 negative regulation of small GTPase mediated signal transduction [PMID:23716719]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:23716719]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:25046117]
EXISTENCE:mutant phenotype:GO:0032007 negative regulation of TOR signaling [PMID:23974112]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:35158208]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:23716719]
EXISTENCE:mutant phenotype:GO:2000785 regulation of autophagosome assembly [PMID:21900499]
GTPase-activating protein (GAP) subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy, and is a subcomplex of the SEA, a coatomer-related complex that associates with the vacuole; Iml1p functions as a GAP for the Rag family GTPase Gtr1p (EGOC subunit) in SEACIT, resulting in the inhibition of TORC1 signaling in response to amino acid deprivation; role in the oxidative stress response
EXISTENCE:direct assay:GO:0004412 homoserine dehydrogenase activity [PMID:11341915]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:4380684]
EXISTENCE:mutant phenotype:GO:0009088 threonine biosynthetic process [PMID:4380684]
EXISTENCE:mutant phenotype:GO:0009090 homoserine biosynthetic process [PMID:4380684]
Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase); dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
EXISTENCE:direct assay:GO:0000417 HIR complex [PMID:16264190|PMID:16303565]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16264190]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9001207]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:16303565|PMID:16264190]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16449659]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:9001207]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:16264190]
EXISTENCE:mutant phenotype:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:1406694]
EXISTENCE:physical interaction:GO:0000417 HIR complex [PMID:16264190]
Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:29519818]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:29519818]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:27708008]
EXISTENCE:mutant phenotype:GO:0031397 negative regulation of protein ubiquitination [PMID:32009536]
EXISTENCE:mutant phenotype:GO:0031442 positive regulation of mRNA 3'-end processing [PMID:32009536]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:29519818]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:27708008]
EXISTENCE:physical interaction:GO:0006378 mRNA polyadenylation [PMID:27708008]
EXISTENCE:physical interaction:GO:0031624 ubiquitin conjugating enzyme binding [PMID:29519818]
EXISTENCE:physical interaction:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:27708008]
Protein implicated in pre-mRNA processing and proteasomal degradation; interacts physically and genetically with mRNA cleavage and polyadenylation factors; mutant is impaired in pre-mRNA cleavage and polyadenylation; associates with several Ub-conjugating enzymes and alters Ub-mediated proteasome activity; localizes to both the nucleus and cytosol; contains a HECT_2 domain; homologous to human ubiquitin-protein ligase, UBE3D, a gene implicated in age-related macular degeneration
EXISTENCE:direct assay:GO:0044715 8-oxo-dGDP phosphatase activity [PMID:23834287]
8-oxo-dGTP diphosphatase of the Nudix hydrolase family; converts diphosphates of damaged forms of thiamin to monophosphates; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition
dolichyl-phosphate-mannose-protein mannosyltransferase
EXISTENCE:direct assay:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:21956107]
EXISTENCE:genetic interaction:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:21231968]
EXISTENCE:mutant phenotype:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:21956107|PMID:8585318]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:8585318]
EXISTENCE:mutant phenotype:GO:0035269 protein O-linked mannosylation [PMID:18182384|PMID:21231968]
EXISTENCE:physical interaction:GO:0097586 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex [PMID:12551906]
Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:22948312]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10209025|PMID:10869431]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:23536705]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:22027892]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:22948312]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:10209025]
Protein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance
Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
hypothetical protein; expressed at both mRNA and protein levels; partially overlaps HMS2
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:9832522]
Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
branched-chain-amino-acid transaminase BAT2
EXISTENCE:direct assay:GO:0004084 branched-chain-amino-acid transaminase activity [PMID:8798704]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21267457|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0009082 branched-chain amino acid biosynthetic process [PMID:8798704]
EXISTENCE:mutant phenotype:GO:0009082 branched-chain amino acid biosynthetic process [PMID:8798704]
EXISTENCE:mutant phenotype:GO:0009083 branched-chain amino acid catabolic process [PMID:8702755]
Cytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10322008]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:12077145]
Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
ARS1022; Autonomously Replicating Sequence
hypothetical protein; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational 'guilt by association' analysis
EXISTENCE:genetic interaction:GO:0042938 dipeptide transport [PMID:17693598]
EXISTENCE:genetic interaction:GO:0071916 dipeptide transmembrane transporter activity [PMID:17693598]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:3275614]
EXISTENCE:mutant phenotype:GO:0015124 allantoate transmembrane transporter activity [PMID:3275614]
EXISTENCE:mutant phenotype:GO:0015719 allantoate transport [PMID:3275614]
EXISTENCE:mutant phenotype:GO:0042938 dipeptide transport [PMID:16429164|PMID:17693598]
EXISTENCE:mutant phenotype:GO:0042939 tripeptide transport [PMID:16429164]
EXISTENCE:mutant phenotype:GO:0071916 dipeptide transmembrane transporter activity [PMID:16429164|PMID:17693598]
Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
EXISTENCE:direct assay:GO:0004650 polygalacturonase activity [PMID:10650215]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11935221|PMID:10028181]
EXISTENCE:direct assay:GO:0045490 pectin catabolic process [PMID:10028181]
Endo-polygalacturonase; pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:12777485]
Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
ARS1023; Autonomously Replicating Sequence
EXISTENCE:genetic interaction:GO:0015795 sorbitol transmembrane transport [PMID:26996892]
EXISTENCE:genetic interaction:GO:0015797 mannitol transmembrane transport [PMID:26996892]
Putative transmembrane polyol transporter; supports growth on and uptake of sorbitol with moderate affinity and mannitol with lower affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; similarity to hexose transporters; expression is repressed by high levels of glucose
EXISTENCE:direct assay:GO:0003939 L-iditol 2-dehydrogenase activity [PMID:8125328]
EXISTENCE:expression pattern:GO:0019318 hexose metabolic process [PMID:8125328]
EXISTENCE:mutant phenotype:GO:0003939 L-iditol 2-dehydrogenase activity [PMID:26996892]
Sorbitol dehydrogenase; protein sequence is 99% identical to the Sor2p sorbitol dehydrogenase; expression is induced in the presence of sorbitol or xylose
ARS1024; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0008643 carbohydrate transport [PMID:12210897]
Alpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; identical to Mph2p; encoded in a subtelomeric position in a region likely to have undergone duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:25942624]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
ARS1025; Autonomously Replicating Sequence
TEL10R; Telomeric region on the right arm of Chromosome X; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats