Saccharomyces cerevisiae S288C chromosome IX, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome IX
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006942.\n\nOn Apr 26, 2011 this sequence version replaced NC_001141.1.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL09L; Telomeric region on the left arm of Chromosome IX; composed of an X element core sequence, X element combinatorial repeats, a long Y' element, and a short terminal stretch of telomeric repeats
Putative Y' element ATP-dependent helicase
ARS902; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; identical to Pau1p
hypothetical protein; classified as pseudogene because there is no ATG at the beginning of this ORF
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:genetic interaction:GO:0005048 signal sequence binding [PMID:8662642]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:8662642]
Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
ARS904; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0004574 oligo-1,6-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0004575 sucrose alpha-glucosidase activity [PMID:24649402]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:genetic interaction:GO:0046352 disaccharide catabolic process [PMID:20562106|PMID:20471265]
EXISTENCE:mutant phenotype:GO:0004574 oligo-1,6-glucosidase activity [PMID:20562106]
Alpha-glucosidase; weak, but broad substrate specificity for alpha-1,4- and alpha-1,6-glucosides; member of IMA isomaltase family; not required for isomaltose utilization, but Ima3p overexpression allows the ima1 null mutant to grow on isomaltose; lower activitiy and thermostability in vitro than Ima2p despite sequence difference of only 3 amino acids; cleaves alpha-1,3 linkage of nigerose and turanose, but not alpha-1,5 of leucrose; identical to IMA4
Possible pseudogene in strain S288C; YIL171W and the adjacent ORF, YIL170W/HXT12, together encode a non-functional member of the hexose transporter family
Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:26928762]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
hypothetical protein, secreted when constitutively expressed; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the cell periphery, SWAT-GFP fusion also localizes to the extracellular region, and mCherry fusion also localizes to the vacuole; S/T rich and highly similar to YOL155C, a putative glucan alpha-1,4-glucosidase; transcript is induced in both high and low pH environments; non-essential gene
Blocked reading frame otherwise encoding L-serine dehydratase
EXISTENCE:genetic interaction:GO:0000316 sulfite transport [PMID:28165463]
EXISTENCE:genetic interaction:GO:0008272 sulfate transport [PMID:28165463]
EXISTENCE:mutant phenotype:GO:0015116 sulfate transmembrane transporter activity [PMID:28165463]
EXISTENCE:mutant phenotype:GO:0072348 sulfur compound transport [PMID:28165463]
EXISTENCE:mutant phenotype:GO:1901682 sulfur compound transmembrane transporter activity [PMID:28165463]
Sulfonate and inorganic sulfur transporter; low affinity sulfate, high affinity sulfite, thiosulfite and major sulfonate transporter; functions as a an H+ symporter; similar to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; non-essential gene
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
hypothetical protein; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene
Nitrilase; member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
hypothetical protein; mRNA identified as translated by ribosome profiling data
EXISTENCE:direct assay:GO:0004564 beta-fructofuranosidase activity [PMID:363706|PMID:3902817]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:6341817]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:6341817]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005987 sucrose catabolic process [PMID:363706|PMID:3902817]
EXISTENCE:direct assay:GO:0051670 inulinase activity [PMID:23104410]
EXISTENCE:direct assay:GO:1902927 inulin catabolic process [PMID:23104410]
EXISTENCE:mutant phenotype:GO:0004564 beta-fructofuranosidase activity [PMID:2834091]
EXISTENCE:mutant phenotype:GO:0005987 sucrose catabolic process [PMID:2834091]
EXISTENCE:mutant phenotype:GO:0010147 fructan catabolic process [PMID:23339519]
EXISTENCE:mutant phenotype:GO:0034484 raffinose catabolic process [PMID:23339519]
Invertase; sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:36113412]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:36113412]
EXISTENCE:direct assay:GO:0070651 nonfunctional rRNA decay [PMID:36113412]
Protein involved in removal of stalled ribosomes; acts as cofactor for Fap1p and Fpr1p in ubiquitination of stalled monosomes and subsequent degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0003988 acetyl-CoA C-acyltransferase activity [PMID:7754706]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:19470242]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:7754706|PMID:8125978]
EXISTENCE:mutant phenotype:GO:0005782 peroxisomal matrix [PMID:8125978]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:12819196]
3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
EXISTENCE:direct assay:GO:0005522 profilin binding [PMID:15923184]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9774458]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:26771880]
EXISTENCE:direct assay:GO:0045010 actin nucleation [PMID:30076201]
EXISTENCE:direct assay:GO:0051016 barbed-end actin filament capping [PMID:15923184]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:15923184]
EXISTENCE:direct assay:GO:0070649 formin-nucleated actin cable assembly [PMID:15923184]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:genetic interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:11740491]
EXISTENCE:genetic interaction:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:12419188]
EXISTENCE:genetic interaction:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521]
EXISTENCE:mutant phenotype:GO:0005522 profilin binding [PMID:15923184]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:23671312]
EXISTENCE:mutant phenotype:GO:0070649 formin-nucleated actin cable assembly [PMID:15371545]
Formin; nucleates the formation of linear actin filaments; involved in processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; activity is regulated by Hof1p and by the Bud14p-Kel1p-Kel2p complex; dephosphorylated and delocalized from the division site in a Glc7p/Ref2p-dependent manner; functionally redundant with BNI1
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
hypothetical protein; overexpression causes cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress; AIM20 has a paralog, SKG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:17882259]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:17882260]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:17882259|PMID:17882260]
EXISTENCE:physical interaction:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:17882259]
Mitochondrial inner membrane protein; required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
Mitochondrial hypothetical protein; originally identified based on homology to Ashbya gossypii and other related yeasts; SWAT-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion also localizes to the endoplasmic reticulum
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:8982460]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:24746795]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:24746795]
Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; involved in cell cycle progression through S phase; UBP7 has a paralog, UBP11, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004368 glycerol-3-phosphate dehydrogenase (quinone) activity [PMID:12032156]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:14576278|PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:12032156]
EXISTENCE:mutant phenotype:GO:0004368 glycerol-3-phosphate dehydrogenase (quinone) activity [PMID:8256521]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:8256521]
EXISTENCE:mutant phenotype:GO:0006071 glycerol metabolic process [PMID:8256521]
Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:8628275]
EXISTENCE:mutant phenotype:GO:0000429 carbon catabolite regulation of transcription from RNA polymerase II promoter [PMID:7496532]
Transcriptional activator involved in maintenance of ion homeostasis; also involved in protection against DNA damage caused by bleomycin and other oxidants; contains a C-terminal leucine-rich repeat
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:21129186]
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:16380387]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15150670]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15150670]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:9705277|PMID:15150670]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:21129186]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:31962153]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:10660069]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:11262194]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:12952889]
EXISTENCE:physical interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:11134337]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:21129186]
Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl1p and Esl2p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; ESL1 has a paralog, ESL2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:15494305|PMID:10783164]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:19605346]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:19605346|PMID:27751725]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11168584|PMID:13680157|PMID:9154825]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:10783164]
EXISTENCE:genetic interaction:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10783164|PMID:11168584]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0005657 replication fork [PMID:15494305]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:10783164|PMID:9154825]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10783164|PMID:11168584|PMID:9154825]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16085704|PMID:16328881]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16085704]
Essential chromatin-associated protein; involved in initiation of DNA replication; required for association of MCM2-7 complex with replication origins; required to stabilize catalytic subunit of DNA polymerase-alpha; coordinates function of replication fork helicase; self-associates through its N-terminal domain
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10085285]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:10085285]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:16027220]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:24152732]
EXISTENCE:genetic interaction:GO:0006355 regulation of DNA-templated transcription [PMID:15692572]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:10085285]
EXISTENCE:genetic interaction:GO:0034398 telomere tethering at nuclear periphery [PMID:10638763]
EXISTENCE:genetic interaction:GO:0043021 ribonucleoprotein complex binding [PMID:15692572]
EXISTENCE:genetic interaction:GO:1901925 negative regulation of protein import into nucleus during spindle assembly checkpoint [PMID:23177738]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:21036941]
EXISTENCE:mutant phenotype:GO:0051300 spindle pole body organization [PMID:16027220]
EXISTENCE:physical interaction:GO:0005816 spindle pole body [PMID:16027220]
EXISTENCE:physical interaction:GO:0043021 ribonucleoprotein complex binding [PMID:15692572]
Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication
ubiquitin-ribosomal 60S subunit protein L40A fusion protein
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:22096102]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:2538753]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:22995916]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:22995916]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:8035826]
Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0004673 protein histidine kinase activity [PMID:8808622]
EXISTENCE:direct assay:GO:0005034 osmosensor activity [PMID:12821642]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10198019|PMID:14665464]
EXISTENCE:direct assay:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:8808622]
EXISTENCE:direct assay:GO:0009927 histidine phosphotransfer kinase activity [PMID:12455952]
EXISTENCE:genetic interaction:GO:0006469 negative regulation of protein kinase activity [PMID:9482735]
EXISTENCE:mutant phenotype:GO:0006469 negative regulation of protein kinase activity [PMID:9482735]
EXISTENCE:mutant phenotype:GO:0009927 histidine phosphotransfer kinase activity [PMID:12455952]
Transmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
ARS907; Autonomously replicating sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:29673596]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:19619494|PMID:19619495]
EXISTENCE:genetic interaction:GO:0031930 mitochondria-nucleus signaling pathway [PMID:30298458]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19619494|PMID:19619495]
Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane; regulates mitophagy and ethanol production during alcoholic fermentation
EXISTENCE:direct assay:GO:0004592 pantoate-beta-alanine ligase activity [PMID:10417331]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
Pantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
kinetochore-associated Ndc80 complex subunit NDC80
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11511347|PMID:27170178]
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:11266451]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:17195848]
EXISTENCE:direct assay:GO:0031262 Ndc80 complex [PMID:11266451|PMID:11179222]
EXISTENCE:mutant phenotype:GO:0000776 kinetochore [PMID:11179222]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11266451]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19893618|PMID:19952112]
EXISTENCE:physical interaction:GO:0031262 Ndc80 complex [PMID:11179222]
Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation
TFIIH/NER complex ATPase/helicase subunit SSL2
EXISTENCE:direct assay:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:8855246]
EXISTENCE:direct assay:GO:0000439 transcription factor TFIIH core complex [PMID:7961739]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:8202161]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408|PMID:27381459]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:8631896]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19818408]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:22751016]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665]
EXISTENCE:direct assay:GO:0097550 transcription preinitiation complex [PMID:22751016]
EXISTENCE:mutant phenotype:GO:0000019 regulation of mitotic recombination [PMID:10713167]
EXISTENCE:mutant phenotype:GO:0001111 RNA polymerase II promoter clearance [PMID:10713451]
EXISTENCE:mutant phenotype:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:10409754]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:34652274]
EXISTENCE:mutant phenotype:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:25775526]
EXISTENCE:mutant phenotype:GO:0010525 regulation of transposition, RNA-mediated [PMID:10713167]
EXISTENCE:mutant phenotype:GO:0015616 DNA translocase activity [PMID:25775526]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:17212653]
Component of RNA polymerase transcription factor TFIIH holoenzyme; acts as dsDNA-dependent translocase in context of TFIIH, unwinds DNA strands during initiation and promotes transcription start site (TSS) scanning; has DNA-dependent ATPase/helicase activity; interacts functionally with TFIIB, has roles in TSS selection and gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to cytosol under hypoxia; homolog of human ERCC3
chaperonin-containing T-complex subunit CCT2
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005832 chaperonin-containing T-complex [PMID:16762366]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:16762366]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16762366]
EXISTENCE:physical interaction:GO:0005832 chaperonin-containing T-complex [PMID:15704212]
Subunit beta of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8846915]
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:8846915]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8846915]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15282802]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:8846915]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:8846915]
Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16546083]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:8658138]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16452144]
EXISTENCE:direct assay:GO:0016035 zeta DNA polymerase complex [PMID:8658138|PMID:23066099|PMID:22711820]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:11313481]
EXISTENCE:direct assay:GO:0070987 error-free translesion synthesis [PMID:12514101]
EXISTENCE:genetic interaction:GO:0042276 error-prone translesion synthesis [PMID:9765213]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:3897795]
Accessory subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; may be involved in meiosis; forms a complex with Rev3p, Pol31p and Pol32p
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:9864365]
EXISTENCE:direct assay:GO:0003786 actin lateral binding [PMID:11457840]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:7844152]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:9864365]
EXISTENCE:direct assay:GO:1904530 negative regulation of actin filament binding [PMID:7844152]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:9864365]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:7844152]
Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005854 nascent polypeptide-associated complex [PMID:27580715]
EXISTENCE:direct assay:GO:1990593 nascent polypeptide-associated complex binding [PMID:27580715]
EXISTENCE:genetic interaction:GO:0042254 ribosome biogenesis [PMID:20691087]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:20691087]
EXISTENCE:mutant phenotype:GO:2000765 regulation of cytoplasmic translation [PMID:27580715]
Ribosome-associated, nascent chain binding factor; binds N-terminal region of nascent peptides during translation; recognizes target proteins via its putative metallopeptidase peptide-binding pocket
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:11179417|PMID:16407407]
Mitochondrial outer membrane hypothetical protein; major constituent of the outer membrane, extending into the intermembrane space; interacts with porin (Por1p) and with Om14p; imported via the presequence pathway involving the TOM and TIM23 complexes, then assembled in the outer membrane by Mim1p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16816427|PMID:14562095]
EXISTENCE:genetic interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:16816427]
EXISTENCE:genetic interaction:GO:0032186 cellular bud neck septin ring organization [PMID:24646733]
EXISTENCE:mutant phenotype:GO:0032186 cellular bud neck septin ring organization [PMID:24646733]
Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A2640
flavin adenine dinucleotide transporter FLX1
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8631763|PMID:14576278|PMID:16823961|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0015230 FAD transmembrane transporter activity [PMID:14555654|PMID:8631763]
EXISTENCE:mutant phenotype:GO:0015883 FAD transport [PMID:14555654]
Mitochondrial flavin adenine dinucleotide transporter; FAD is a synthesis product of riboflavin; human homolog SLC25A32 is implicated in multiple acyl-CoA dehydrogenase deficiency (MADD) or glutaric aciduria type II (GAII), and can complement yeast null mutant
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:27374275]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:27374275]
Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0070987 error-free translesion synthesis [PMID:19496932]
EXISTENCE:direct assay:GO:0097196 Shu complex [PMID:23575680]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:15654096]
EXISTENCE:genetic interaction:GO:0043007 maintenance of rDNA [PMID:21372173]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0000730 DNA recombinase assembly [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0035861 site of double-strand break [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:11470404]
EXISTENCE:physical interaction:GO:0097196 Shu complex [PMID:15654096]
Subunit of the Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p anti-recombinase function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
forkhead family transcription factor FKH1
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10894548|PMID:11562353]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10894548]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10894548|PMID:11562353]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:24504085]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:22265405|PMID:24504085]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10747051|PMID:20847055|PMID:22932476]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:10683251]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:24504085|PMID:19158363]
EXISTENCE:genetic interaction:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10894548]
EXISTENCE:genetic interaction:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10894548|PMID:11562353]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:17283050]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:12702877]
EXISTENCE:genetic interaction:GO:0007535 donor selection [PMID:12183363|PMID:16809780]
EXISTENCE:genetic interaction:GO:0034244 negative regulation of transcription elongation by RNA polymerase II [PMID:12702877]
EXISTENCE:genetic interaction:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10894548|PMID:17898805]
EXISTENCE:genetic interaction:GO:0090055 positive regulation of silent mating-type cassette heterochromatin formation [PMID:10747051|PMID:18045995]
EXISTENCE:genetic interaction:GO:0090282 positive regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:11562353|PMID:10747051|PMID:10894548|PMID:10959837]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:10747051|PMID:10894548]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:17283050]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:12702877]
EXISTENCE:mutant phenotype:GO:0007535 donor selection [PMID:12183363|PMID:16809780|PMID:27257873]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:12702877]
EXISTENCE:mutant phenotype:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:22265405]
EXISTENCE:mutant phenotype:GO:0034244 negative regulation of transcription elongation by RNA polymerase II [PMID:12702877]
EXISTENCE:mutant phenotype:GO:0090055 positive regulation of silent mating-type cassette heterochromatin formation [PMID:10747051]
EXISTENCE:mutant phenotype:GO:0090419 negative regulation of transcription involved in G2/M transition of mitotic cell cycle [PMID:10747051|PMID:18045995]
EXISTENCE:mutant phenotype:GO:1903468 positive regulation of DNA replication initiation [PMID:26728715]
Forkhead family transcription factor; rate-limiting replication origin activator; evolutionarily conserved lifespan regulator; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; regulates transcription elongation, chromatin silencing at mating loci, expression of G2/M phase genes; facilitates clustering, activation of early-firing replication origins; binds HML recombination enhancer, regulates donor preference during mating-type switching
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
Zinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; regulates utilization of fatty acids and accumulation of lipids
ARS909; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11854408]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12972564]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12972564]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0000902 cell morphogenesis [PMID:11854408]
EXISTENCE:mutant phenotype:GO:0007114 cell budding [PMID:11854408]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:12972564]
Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
ARS910; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0097361 CIA complex [PMID:22678362]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:22678362]
Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
hypothetical protein; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
RSC chromatin remodeling complex ATPase subunit STH1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1396591|PMID:1549132]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:8980231]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0015616 DNA translocase activity [PMID:12183366]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:20126658]
EXISTENCE:direct assay:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:9799253|PMID:12183366]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:12697820]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:24674626]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16024655]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:12072455]
EXISTENCE:mutant phenotype:GO:0031055 chromatin remodeling at centromere [PMID:12697820]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:10320476]
ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0009353 mitochondrial oxoglutarate dehydrogenase complex [PMID:2072900]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:11926067|PMID:15692048]
Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:24187129]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10617610|PMID:26928762]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10617610|PMID:24868093]
EXISTENCE:genetic interaction:GO:0004806 triglyceride lipase activity [PMID:24187129]
EXISTENCE:genetic interaction:GO:0019433 triglyceride catabolic process [PMID:24187129]
EXISTENCE:mutant phenotype:GO:0000140 acylglycerone-phosphate reductase activity [PMID:10617610]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:24187129]
EXISTENCE:mutant phenotype:GO:0006654 phosphatidic acid biosynthetic process [PMID:10617610]
Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase; NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase involved in phosphatidic acid biosynthesis; lipid droplet triacylglycerol lipase involved in mobilization of non-polar lipids; found in lipid particles, endoplasmic reticulum and mitochondrial outer membrane; forms NADPH-regulated channel in mitochondrial outer membrane; required for spore germination; role in cell wall biosynthesis; capable of metabolizing steroid hormones
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11958935]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15972461]
Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p); may participate in DNA replication; promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated; SIM1 has a paralog, SUN4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:22959267]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:9927449]
EXISTENCE:mutant phenotype:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:9927449]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22959267|PMID:9927449]
DNA-binding transcriptional activator; involved in cell cycle regulation; overexpression promotes recovery from pheromone induced arrest via CLN1/2 transcription, induction of of IME1 during sporulation, and suppression of stress sensitivity resulting from mutation of the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; phosphorylated form may be ubiquitinated by Dma2p; potential Cdc28p substrate; regulated by Swi4/6 cell-cycle box binding factor (SBF)
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15215105|PMID:34887841]
EXISTENCE:genetic interaction:GO:1990573 potassium ion import across plasma membrane [PMID:17189489]
EXISTENCE:mutant phenotype:GO:0008324 cation transmembrane transporter activity [PMID:23106982]
EXISTENCE:mutant phenotype:GO:0015565 threonine efflux transmembrane transporter activity [PMID:34887841]
EXISTENCE:mutant phenotype:GO:0032973 amino acid export across plasma membrane [PMID:34887841]
EXISTENCE:mutant phenotype:GO:0042910 xenobiotic transmembrane transporter activity [PMID:15215105|PMID:15649438]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15215105|PMID:15649438]
EXISTENCE:mutant phenotype:GO:0060003 copper ion export [PMID:23106982]
EXISTENCE:mutant phenotype:GO:1990573 potassium ion import across plasma membrane [PMID:17189489]
Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11302822]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
EXISTENCE:mutant phenotype:GO:0015562 efflux transmembrane transporter activity [PMID:15649438]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15649438]
Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in spore wall assembly; sequence similarity to DTR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication
ARS911; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12455971]
EXISTENCE:genetic interaction:GO:0009272 fungal-type cell wall biogenesis [PMID:12455971]
EXISTENCE:mutant phenotype:GO:0007265 Ras protein signal transduction [PMID:1649384]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:12455971]
EXISTENCE:mutant phenotype:GO:0030695 GTPase regulator activity [PMID:1649384]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12455971]
Transcription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:10526184]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:10207081]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10207081]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:10207081]
EXISTENCE:genetic interaction:GO:0045921 positive regulation of exocytosis [PMID:8852836|PMID:19955214]
EXISTENCE:genetic interaction:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:12810699]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:10588647|PMID:8852836]
EXISTENCE:mutant phenotype:GO:0045921 positive regulation of exocytosis [PMID:10588647]
EXISTENCE:mutant phenotype:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:12810699]
EXISTENCE:physical interaction:GO:0045921 positive regulation of exocytosis [PMID:19955214]
Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241]
EXISTENCE:mutant phenotype:GO:0004400 histidinol-phosphate transaminase activity [PMID:14190241]
Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:21576266]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:7642562|PMID:7744966]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:10684247|PMID:14960378]
EXISTENCE:direct assay:GO:0061912 selective autophagy [PMID:32029894]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:10952996]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906|PMID:11739405]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:21576266]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:17347149]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342|PMID:7744966|PMID:9359887|PMID:9802895|PMID :15574330]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:9359887|PMID:7744966]
EXISTENCE:mutant phenotype:GO:0097064 ncRNA export from nucleus [PMID:11352936]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:9315631|PMID:16407407]
EXISTENCE:genetic interaction:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:23104570]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:18768136]
EXISTENCE:mutant phenotype:GO:0051027 DNA transport [PMID:19056337]
Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication
mitogen-activated protein kinase tyrosine protein phosphatase SDP1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11923319]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11923319]
EXISTENCE:direct assay:GO:0017017 MAP kinase tyrosine/serine/threonine phosphatase activity [PMID:12220658]
EXISTENCE:direct assay:GO:0033550 MAP kinase tyrosine phosphatase activity [PMID:11923319|PMID:17495930]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:11923319]
Stress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0016575 histone deacetylation [PMID:11711434]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:2986105]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
EXISTENCE:mutant phenotype:GO:0004129 cytochrome-c oxidase activity [PMID:2986105]
EXISTENCE:mutant phenotype:GO:0050421 nitrite reductase (NO-forming) activity [PMID:18388202]
Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:24865971|PMID:26826131]
EXISTENCE:mutant phenotype:GO:0018064 protein-L-histidine N-tele-methyltransferase activity [PMID:20864530]
EXISTENCE:mutant phenotype:GO:0042038 peptidyl-histidine methylation, to form tele-methylhistidine [PMID:20864530]
EXISTENCE:mutant phenotype:GO:0045903 positive regulation of translational fidelity [PMID:24865971]
AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of multiple proteins, including ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0030127 COPII vesicle coat [PMID:8004676]
EXISTENCE:direct assay:GO:0090110 COPII-coated vesicle cargo loading [PMID:12941277|PMID:14627716|PMID:15665868]
EXISTENCE:mutant phenotype:GO:0005048 signal sequence binding [PMID:12941277]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11694599]
Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:1657152]
EXISTENCE:direct assay:GO:0003873 6-phosphofructo-2-kinase activity [PMID:1322693]
EXISTENCE:mutant phenotype:GO:0003873 6-phosphofructo-2-kinase activity [PMID:1657152]
EXISTENCE:mutant phenotype:GO:0006003 fructose 2,6-bisphosphate metabolic process [PMID:1657152]
6-phosphofructo-2-kinase; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:11434459]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11434459|PMID:11564880]
EXISTENCE:direct assay:GO:0019207 kinase regulator activity [PMID:11404483]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
EXISTENCE:direct assay:GO:0034973 Sid2-Mob1 complex [PMID:9528782]
EXISTENCE:direct assay:GO:0044732 mitotic spindle pole body [PMID:11564880]
EXISTENCE:genetic interaction:GO:0007096 regulation of exit from mitosis [PMID:9436989]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:11564880]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:9436989]
EXISTENCE:physical interaction:GO:0034973 Sid2-Mob1 complex [PMID:9528782]
Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
phosphatidylinositol 4,5-bisphosphate-binding protein
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:15689497|PMID:21119626]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15372071|PMID:15689497|PMID:17101780|PMID:16622836]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668|PMID:21451250]
EXISTENCE:direct assay:GO:0046625 sphingolipid binding [PMID:21119626]
EXISTENCE:genetic interaction:GO:0001558 regulation of cell growth [PMID:15689497]
EXISTENCE:genetic interaction:GO:0016197 endosomal transport [PMID:21451250]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:15372071|PMID:15689497]
EXISTENCE:genetic interaction:GO:0051017 actin filament bundle assembly [PMID:15689497]
EXISTENCE:genetic interaction:GO:0070941 eisosome assembly [PMID:21451250]
EXISTENCE:genetic interaction:GO:0072659 protein localization to plasma membrane [PMID:22307609]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:15689497]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:15689497]
EXISTENCE:physical interaction:GO:0031929 TOR signaling [PMID:15689497]
Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
hypothetical protein; completely overlaps the verified gene SLM1; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; mRNA identified as translated by ribosome profiling data
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:12228251]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:19426738]
EXISTENCE:mutant phenotype:GO:0000493 box H/ACA snoRNP assembly [PMID:12228251]
EXISTENCE:physical interaction:GO:0000493 box H/ACA snoRNP assembly [PMID:12228251]
Chaperone protein; required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing; functions as an RNA mimic; forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones; relocalizes to the cytosol in response to hypoxia
2-(3-amino-3-carboxypropyl)histidine synthase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:15485916]
Protein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); forms a complex with Dph2p that catalyzes the first step of diphthamide biosynthesis
Regulatory subunit of dolichyl phosphate mannose (DPM) synthase; identified based on comparisons of the genome sequences of six Saccharomyces species; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
ARS912; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:10611226]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:9343412]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9343412|PMID:22842922]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10611226|PMID:11340165|PMID:24204289|PMID:9343412]
Transcriptional repressor; binds promoter sequences of cyclin genes, CYS3, and SMF2; not expressed during log phase of growth, but induced by stress or starvation during mitosis, and late in meiosis; represses 15% of all yeast genes as cells transition to quiescence; important for maintaining G1 arrest and for longevity of quiescent cells; member of Swi4p/Mbp1p family; phosphorylated by Cdc28p; relative distribution to nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:2493265|PMID:26928762]
EXISTENCE:direct assay:GO:0004339 glucan 1,4-alpha-glucosidase activity [PMID:2493265]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0005980 glycogen catabolic process [PMID:11486014]
Intracellular sporulation-specific glucoamylase; involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11096112]
EXISTENCE:genetic interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:11096112]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:11096112]
Mitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
glucose-induced degradation complex subunit FYV10
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:16872538|PMID:18508925]
EXISTENCE:mutant phenotype:GO:0043066 negative regulation of apoptotic process [PMID:18355271]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:22044534]
Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin
25S rRNA (uracil2634-N3)-methyltransferase
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:24335083|PMID:14562095]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:21858014]
EXISTENCE:mutant phenotype:GO:0070042 rRNA (uridine-N3-)-methyltransferase activity [PMID:24335083]
EXISTENCE:mutant phenotype:GO:0070475 rRNA base methylation [PMID:24335083]
Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:17978096|PMID:9885245|PMID:11739778|PMID:13679512|PM ID:12956961]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10087264|PMID:9885245]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:genetic interaction:GO:0120133 negative regulation of actin cortical patch assembly [PMID:9885245]
EXISTENCE:genetic interaction:GO:1900186 negative regulation of clathrin-dependent endocytosis [PMID:13679512]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:12952930]
EXISTENCE:mutant phenotype:GO:0031333 negative regulation of protein-containing complex assembly [PMID:11739778]
EXISTENCE:mutant phenotype:GO:1900186 negative regulation of clathrin-dependent endocytosis [PMID:12956961]
Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1
EXISTENCE:curator inference:GO:0009085 lysine biosynthetic process [PMID:17223711]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0047046 homoisocitrate dehydrogenase activity [PMID:17223711]
Homo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
mitochondrial 37S ribosomal protein RSM25
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
rRNA-binding ribosome biosynthesis protein UTP25
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20528918|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:20528918|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:20528918]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:20884785]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:20528918]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:20884785|PMID:20528918]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:20884785|PMID:19806183|PMID:20528918]
EXISTENCE:physical interaction:GO:0032040 small-subunit processome [PMID:20528918]
Nucleolar protein; required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15585575]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:15585575]
EXISTENCE:direct assay:GO:0097038 perinuclear endoplasmic reticulum [PMID:15585575]
EXISTENCE:genetic interaction:GO:0000921 septin ring assembly [PMID:17984322]
EXISTENCE:genetic interaction:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:34617598]
EXISTENCE:genetic interaction:GO:0160031 endoplasmic reticulum membrane biogenesis [PMID:34617598]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:17984322]
EXISTENCE:mutant phenotype:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:34617598]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:22685415]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:32513868]
EXISTENCE:mutant phenotype:GO:0036228 protein localization to nuclear inner membrane [PMID:15911569]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:15585575|PMID:17984322]
EXISTENCE:mutant phenotype:GO:0160031 endoplasmic reticulum membrane biogenesis [PMID:34617598]
Integral ER membrane protein with type-III transmembrane domains; promotes ER membrane expansion by inhibiting the Nem1-Spo7 phosphatase complex, resulting in reduced dephosphorylation and activation of Pah1p; maintains homeostasis during ER stress; required for post-translational SRP-independent protein translocation into the ER; necessary for efficient targeting of the Trm1p to the inner nuclear membrane; mutations cause defects in cortical ER morphology; member of the SERINC superfamily
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:21777356]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:26928762]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:21777356]
hypothetical protein found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11274162|PMID:27246536|PMID:26928762]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:25266154]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:25266154]
EXISTENCE:mutant phenotype:GO:0043937 regulation of sporulation [PMID:25266154]
Vacuolar amino acid transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:25124039]
hypothetical protein; mitochondrial protein that physically interacts with Tim23p; null mutant displays reduced respiratory growth
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9090056]
Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:24358376]
EXISTENCE:mutant phenotype:GO:0004407 histone deacetylase activity [PMID:11024051]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:11024051]
EXISTENCE:mutant phenotype:GO:0043709 cell adhesion involved in single-species biofilm formation [PMID:18202364]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17210643|PMID:20398213]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:20398213]
EXISTENCE:mutant phenotype:GO:2000217 regulation of invasive growth in response to glucose limitation [PMID:18202364]
Component of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes
phosphopantothenate--cysteine ligase CAB2
EXISTENCE:direct assay:GO:0004632 phosphopantothenate--cysteine ligase activity [PMID:30653991]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:direct assay:GO:1990143 CoA-synthesizing protein complex [PMID:23789928]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19266201]
Phosphopantothenoylcysteine synthetase (PPCS); catalyzes the second step of coenzyme A biosynthesis from pantothenate; subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) that contains: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p subunits; null mutant lethality is complemented by human homolog PPCS and by E. coli coaBC, a bifunctional enzyme with PPCS activity
YILWTy3-1; Ty3 element, LTR retrotransposon of the Gypsy (Metaviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; transposition is induced upon exposure to mating pheromone
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
EXISTENCE:direct assay:GO:0004652 polynucleotide adenylyltransferase activity [PMID:15935759]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11489916]
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:15828860|PMID:15935759]
EXISTENCE:direct assay:GO:0043629 ncRNA polyadenylation [PMID:15828860]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:genetic interaction:GO:0004652 polynucleotide adenylyltransferase activity [PMID:15935759]
EXISTENCE:genetic interaction:GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing [PMID:17410208]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:18007593]
EXISTENCE:genetic interaction:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:15935758]
EXISTENCE:genetic interaction:GO:0071037 nuclear polyadenylation-dependent snRNA catabolic process [PMID:15935758]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome
EXISTENCE:direct assay:GO:0004829 threonine-tRNA ligase activity [PMID:8143729]
EXISTENCE:direct assay:GO:0006435 threonyl-tRNA aminoacylation [PMID:8143729]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:2995918]
Threonyl-tRNA synthetase; essential cytoplasmic protein; human homolog TARS can complement yeast null mutant
ARS901; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
Protein that associates with the large mitoribosomal subunit; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17101773]
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:21435344]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:27184763]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10532354]
EXISTENCE:mutant phenotype:GO:0006901 vesicle coating [PMID:9463377]
EXISTENCE:mutant phenotype:GO:0030126 COPI vesicle coat [PMID:9463377]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17101773]
EXISTENCE:physical interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17101773]
Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
proteasome regulatory particle base subunit RPN2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15210724|PMID:22842922]
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:9741626]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:17911101]
EXISTENCE:mutant phenotype:GO:0004175 endopeptidase activity [PMID:7565784]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:7565784]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:10688918]
Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
EXISTENCE:genetic interaction:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
EXISTENCE:mutant phenotype:GO:0004617 phosphoglycerate dehydrogenase activity [PMID:12525494]
EXISTENCE:mutant phenotype:GO:0009070 serine family amino acid biosynthetic process [PMID:12525494]
EXISTENCE:mutant phenotype:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER33 has a paralog, SER3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16740482]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0033235 positive regulation of protein sumoylation [PMID:23326245]
EXISTENCE:mutant phenotype:GO:0090173 regulation of synaptonemal complex assembly [PMID:16740482]
Meiosis-specific protein essential for chromosome synapsis; involved in completion of nuclear divisions during meiosis; induced early in meiosis
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:17027027]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:2107981]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23841450|PMID:24390141]
EXISTENCE:genetic interaction:GO:0051598 meiotic recombination checkpoint signaling [PMID:10848609]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:2107981|PMID:8207053]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:2653960]
EXISTENCE:physical interaction:GO:0000800 lateral element [PMID:9060462]
Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein-DNA filaments
EXISTENCE:direct assay:GO:0008180 COP9 signalosome [PMID:12446563]
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:12186635|PMID:12446563]
Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:9305894]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:31053642]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9305894]
EXISTENCE:mutant phenotype:GO:0061668 mitochondrial ribosome assembly [PMID:31053642]
Acidic protein of the mitochondrial matrix; binds unassembled large subunit proteins during ribosome assembly; subunit of a complex containing Mrx6p, Pim1p, and Pet20p that may regulate mtDNA replication; related to the human complement receptor gC1q-R
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:7615633|PMID:8978675]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:15835919|PMID:22114349]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:19073882]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:19073882|PMID:26928762]
EXISTENCE:direct assay:GO:0035544 negative regulation of SNARE complex assembly [PMID:15835919|PMID:22114349]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:genetic interaction:GO:0035544 negative regulation of SNARE complex assembly [PMID:22114349]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0051601 exocyst localization [PMID:19073882]
EXISTENCE:physical interaction:GO:0000149 SNARE binding [PMID:15835919|PMID:22114349]
EXISTENCE:physical interaction:GO:0035544 negative regulation of SNARE complex assembly [PMID:15835919|PMID:22114349]
Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
ribonucleotide-diphosphate reductase subunit RNR3
EXISTENCE:direct assay:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:11893751]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12732713]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0009263 deoxyribonucleotide biosynthetic process [PMID:5459124]
Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:11038183|PMID:15809300|PMID:16407407]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16968746]
EXISTENCE:genetic interaction:GO:0016559 peroxisome fission [PMID:18445678]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:11038182|PMID:11038183]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:16968746]
Protein involved in mitochondrial fission and peroxisome abundance; may have a distinct role in tethering protein aggregates to mitochondria in order to retain them in the mother cell; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:20510667]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:19160456]
Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:12082158]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:9636166]
hypothetical protein; involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; relocalizes to the cytosol in response to hypoxia; not essential for viability
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407|PMID:9210376]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:11248049]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:10377407]
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
EXISTENCE:physical interaction:GO:0030619 U1 snRNA binding [PMID:1387202]
Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1-70K, which has been linked to several types of autoimmune and neurodegenerative diseases
ARS923; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24360837]
Mitochondrial hypothetical protein; required for respiratory growth; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
ARS914; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0006112 energy reserve metabolic process [PMID:20567505]
hypothetical protein; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication
ARS915; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:9023104]
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:16533810]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:21278159]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16533810|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:21278159]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16533810|PMID:21278159]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16533810]
Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
hypothetical protein; expressed at both mRNA and protein levels
EXISTENCE:direct assay:GO:0000121 glycerol-1-phosphatase activity [PMID:8662716]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:27385335|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006114 glycerol biosynthetic process [PMID:11058591]
Constitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication
Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11003673]
EXISTENCE:genetic interaction:GO:0032543 mitochondrial translation [PMID:15164357]
EXISTENCE:mutant phenotype:GO:0009097 isoleucine biosynthetic process [PMID:11442631]
Mitochondrial protein required for transamination of isoleucine; but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; induction of expression in response to stress is mediated by a Hog1p-regulated antisense RNA and gene looping; interacts genetically with mitochondrial ribosomal protein genes; MMF1 has a paralog, HMF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:12407105]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16611745]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:12407105]
EXISTENCE:genetic interaction:GO:0005979 regulation of glycogen biosynthetic process [PMID:11602261]
EXISTENCE:genetic interaction:GO:0005981 regulation of glycogen catabolic process [PMID:11602261]
EXISTENCE:mutant phenotype:GO:0031647 regulation of protein stability [PMID:16611745]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:16611745]
Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:20637498]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0019408 dolichol biosynthetic process [PMID:20637498]
Probable polyprenol reductase; catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human homolog SRD5A3 confer CDG (Congenital Disorders of Glycosylation); human SRD5A3 can complement yeast null mutant
putative aminophospholipid-translocating P4-type ATPase NEO1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12960419]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15314152]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:30981741]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15314152]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:30981741]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:12960419]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:15314152]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:15314152]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:27235400]
Phospholipid translocase (flippase); involved in generating phospholipid asymmetry in plasma membrane; involved in endocytosis, vacuolar biogenesis and Golgi-to-ER vesicle-mediated transport; localizes to endosomes and Golgi apparatus; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7592711]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:7592711]
Plasma membrane hypothetical protein; targeted to vacuole via AP-3 pathway; truncation and overexpression suppresses lethality of G-alpha protein deficiency
hypothetical protein; identified by expression profiling and mass spectrometry
ubiquitin-binding SDF ubiquitin ligase complex subunit MET30
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10637232]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:9716410]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:direct assay:GO:0043224 nuclear SCF ubiquitin ligase complex [PMID:10637232]
EXISTENCE:direct assay:GO:0046685 response to arsenic-containing substance [PMID:15689486]
EXISTENCE:direct assay:GO:0046686 response to cadmium ion [PMID:15689486]
EXISTENCE:genetic interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:9716410]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:15870262]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:15870262]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:9716410]
F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p
putative protein phosphatase regulator PIG2
EXISTENCE:physical interaction:GO:0005979 regulation of glycogen biosynthetic process [PMID:9046081]
Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12627398]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9677411]
EXISTENCE:genetic interaction:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:9677411]
ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
EXISTENCE:direct assay:GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H [PMID:14930|PMID:27694803]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:27694803]
Cytochrome b reductase and NADH-dependent reductase for Dph3p; required for diphthamide synthesis and tRNA wobble uridine modification; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:17918780]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16643908|PMID:16823961|PMID:24769239|PMID:17918780]
EXISTENCE:genetic interaction:GO:0015976 carbon utilization [PMID:17918780]
EXISTENCE:mutant phenotype:GO:0004740 pyruvate dehydrogenase (acetyl-transferring) kinase activity [PMID:18180296|PMID:16643908]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:16643908]
EXISTENCE:mutant phenotype:GO:1901524 regulation of mitophagy [PMID:31548421]
Mitochondrial protein kinase; involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:16107716]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:18156177]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:18156177]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:18156177]
BAR domain protein that localizes to early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15273328|PMID:26432634]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15273328|PMID:26432634|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:17724120]
EXISTENCE:genetic interaction:GO:0055088 lipid homeostasis [PMID:20016074|PMID:26432634]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:17724120|PMID:20016074]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:20016074|PMID:26432634]
Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17989219]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17989219]
GPI-glycan remodelase; conserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; functional ortholog of mammalian GPI-glycan remodelase PGAP5; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11889048]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:26952104]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16926149]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:26952104]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:10490603]
Component of the CCR4-NOT core complex, involved in mRNA decapping; involved in transcription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:17101777]
Pheromone-regulated protein; predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10825197]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:27143390]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:10825197|PMID:27143390]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10825197]
EXISTENCE:mutant phenotype:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:20395639]
EXISTENCE:mutant phenotype:GO:0071244 cellular response to carbon dioxide [PMID:22253597]
EXISTENCE:mutant phenotype:GO:0071400 cellular response to oleic acid [PMID:20395639]
Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8226802]
EXISTENCE:direct assay:GO:0005956 protein kinase CK2 complex [PMID:8135547]
EXISTENCE:direct assay:GO:0006356 regulation of transcription by RNA polymerase I [PMID:11551505]
EXISTENCE:direct assay:GO:0006359 regulation of transcription by RNA polymerase III [PMID:11551505]
EXISTENCE:direct assay:GO:0006974 cellular response to DNA damage stimulus [PMID:11551505]
EXISTENCE:direct assay:GO:0034456 UTP-C complex [PMID:17515605]
EXISTENCE:genetic interaction:GO:0007535 donor selection [PMID:22496671]
Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:1315784]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:1315784|PMID:22842922]
EXISTENCE:direct assay:GO:0008290 F-actin capping protein complex [PMID:14769858]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:14769858]
EXISTENCE:direct assay:GO:0051016 barbed-end actin filament capping [PMID:14769858|PMID:1315784]
EXISTENCE:mutant phenotype:GO:0008290 F-actin capping protein complex [PMID:14769858]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0051015 actin filament binding [PMID:14769858]
Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
cAMP-dependent protein kinase regulatory subunit BCY1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:26403272]
EXISTENCE:direct assay:GO:0004862 cAMP-dependent protein kinase inhibitor activity [PMID:3037314]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11134339|PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2831892]
EXISTENCE:direct assay:GO:0046580 negative regulation of Ras protein signal transduction [PMID:3037314]
EXISTENCE:genetic interaction:GO:0045762 positive regulation of adenylate cyclase activity [PMID:2981630|PMID:3891097]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:12782684]
EXISTENCE:mutant phenotype:GO:0004862 cAMP-dependent protein kinase inhibitor activity [PMID:6292221]
EXISTENCE:mutant phenotype:GO:0010603 regulation of cytoplasmic mRNA processing body assembly [PMID:21925385]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26947009]
EXISTENCE:mutant phenotype:GO:0046580 negative regulation of Ras protein signal transduction [PMID:3037314]
Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation; regulator of meiotic commitment
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:30575729]
EXISTENCE:direct assay:GO:0003711 transcription elongation factor activity [PMID:31313851]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10713161]
EXISTENCE:direct assay:GO:0008234 cysteine-type peptidase activity [PMID:10713161]
EXISTENCE:direct assay:GO:0016926 protein desumoylation [PMID:10713161]
EXISTENCE:direct assay:GO:0016929 deSUMOylase activity [PMID:10713161]
EXISTENCE:mutant phenotype:GO:0006276 plasmid maintenance [PMID:10713161|PMID:11333221]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:10713162]
EXISTENCE:mutant phenotype:GO:0016926 protein desumoylation [PMID:10713161]
EXISTENCE:mutant phenotype:GO:0016929 deSUMOylase activity [PMID:10713161]
EXISTENCE:mutant phenotype:GO:0030261 chromosome condensation [PMID:11333221]
Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins; plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate; human homolog PML implicated in promyelocytic leukemia can partially complement yeast null mutant
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873066]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:11641273]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11641273]
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:17051211]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11641273]
EXISTENCE:direct assay:GO:0030970 retrograde protein transport, ER to cytosol [PMID:32588820]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:25918226]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11641273|PMID:15252059]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:32588820]
Membrane-embedded ubiquitin-protein ligase and retrotranslocase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); aids Cdc48p in the extraction of faulty membrane proteins; targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and Sbh2p, a transmembrane domain-containing substrate (ERAD-M); C-terminal element, conserved in human ortholog MARCH6, determines substrate selectivity
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
Protein involved in targeting mitochondrial membrane protein precursors to mitochondrial translocation system; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has a paralog, YPR071W, that arose from a single-locus duplication
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:9990856]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:28077952|PMID:10028182|PMID:22932476]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:28077952]
EXISTENCE:direct assay:GO:0030892 mitotic cohesin complex [PMID:9990856]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:9990856]
Subunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability
hypothetical protein; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16760462|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005385 zinc ion transmembrane transporter activity [PMID:16760462]
EXISTENCE:mutant phenotype:GO:0006829 zinc ion transport [PMID:16760462]
Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family
EXISTENCE:direct assay:GO:0001405 PAM complex, Tim23 associated import motor [PMID:14517234|PMID:14638855]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:1396562|PMID:18400944]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:10824101|PMID:11344168]
EXISTENCE:mutant phenotype:GO:0051087 chaperone binding [PMID:11344168]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:7809127]
Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane
DNA-directed RNA polymerase II core subunit RPB3
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15520468]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2186966|PMID:2183013]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:2685562]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:16537912]
RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
ARS918; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
1-(5-phosphoribosyl)-5- ((5-phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase HIS6
EXISTENCE:genetic interaction:GO:0000105 histidine biosynthetic process [PMID:9332345]
EXISTENCE:genetic interaction:GO:0003949 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase activity [PMID:9332345]
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241|PMID:9332345]
Enzyme that catalyzes the fourth step in the histidine pathway; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15078877|PMID:15178413]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15078877|PMID:15178413]
Protein required for pre-rRNA processing; also required for 40S ribosomal subunit assembly
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
glucose-induced degradation complex subunit VID28
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:15358789]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:12586695]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616|PMID:15358789]
GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:9450961]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9450961]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:22635919]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:9450961]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:12105220]
Ribosome-associated protein; proposed to act in protein synthesis, nuclear pore complex biogenesis and maintenance as well as protein folding and prion maintenance; has similarity to the mammalian BAG-1 protein
EXISTENCE:direct assay:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:6345506]
EXISTENCE:direct assay:GO:0004190 aspartic-type endopeptidase activity [PMID:9249020]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:3124102]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10438739]
EXISTENCE:direct assay:GO:0043171 peptide catabolic process [PMID:9249020]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:7050666]
Aspartyl protease secreted to periplasmic space of mating type a cell; helps cells monitor availability of mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
hypothetical protein; null mutant shows improved incorporation efficiency of noncanonical amino acids in place of amber stop codon
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:30700649]
EXISTENCE:genetic interaction:GO:0000033 alpha-1,3-mannosyltransferase activity [PMID:10521541]
EXISTENCE:genetic interaction:GO:0006493 protein O-linked glycosylation [PMID:10521541]
EXISTENCE:mutant phenotype:GO:0000033 alpha-1,3-mannosyltransferase activity [PMID:10521541]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:10521541]
Alpha-1,3-mannosyltransferase; adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation
ATP-binding cassette multidrug transporter PDR11
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:33375075]
EXISTENCE:direct assay:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:28922409]
EXISTENCE:genetic interaction:GO:0035376 sterol import [PMID:12077145|PMID:21110944]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:12077145]
EXISTENCE:mutant phenotype:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:28922409]
ATP-binding cassette (ABC) transporter; multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth; PDR11 has a paralog, AUS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
Cell wall mannoprotein; member of Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; TIR3 has a paralog, TIR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2408019|PMID:10681558]
EXISTENCE:direct assay:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:10681558]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:15051715]
EXISTENCE:mutant phenotype:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:10681558]
Nuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:20884318]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12101098]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:10898986]
EXISTENCE:direct assay:GO:0007004 telomere maintenance via telomerase [PMID:10898986]
EXISTENCE:direct assay:GO:0033677 DNA/RNA helicase activity [PMID:16884717]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:16884717]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:20884318]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:8978029]
Component of the telomerase holoenzyme; involved in telomere replication; synthesis of the full-length protein results from a programmed +1 ribosomal frameshift
EXISTENCE:direct assay:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:direct assay:GO:0031957 very long-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:genetic interaction:GO:0001676 long-chain fatty acid metabolic process [PMID:7962057]
Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
ARS919; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14551258|PMID:14562095]
EXISTENCE:direct assay:GO:0031386 protein tag [PMID:14551258|PMID:10713047]
EXISTENCE:direct assay:GO:0032447 protein urmylation [PMID:10713047|PMID:14555475]
EXISTENCE:direct assay:GO:0034599 cellular response to oxidative stress [PMID:21209336]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:14551258]
EXISTENCE:genetic interaction:GO:0007114 cell budding [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0002143 tRNA wobble position uridine thiolation [PMID:19145231|PMID:18664566|PMID:19151091]
EXISTENCE:mutant phenotype:GO:0031386 protein tag [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0032447 protein urmylation [PMID:14555475]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:14555475]
Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19446323]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11489916]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19446323]
EXISTENCE:direct assay:GO:0070682 proteasome regulatory particle assembly [PMID:19446322]
EXISTENCE:genetic interaction:GO:0070682 proteasome regulatory particle assembly [PMID:19446322|PMID:19446323]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19446322|PMID:19446323]
Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress; ortholog of human p27
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16291748|PMID:24769239]
EXISTENCE:direct assay:GO:0035352 NAD transmembrane transport [PMID:16291748]
EXISTENCE:direct assay:GO:0051724 NAD transmembrane transporter activity [PMID:16291748]
EXISTENCE:genetic interaction:GO:0035352 NAD transmembrane transport [PMID:16291748]
EXISTENCE:genetic interaction:GO:0051724 NAD transmembrane transporter activity [PMID:16291748]
EXISTENCE:mutant phenotype:GO:0005477 pyruvate secondary active transmembrane transporter activity [PMID:12887330]
EXISTENCE:mutant phenotype:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:12887330]
EXISTENCE:mutant phenotype:GO:0035352 NAD transmembrane transport [PMID:16291748]
EXISTENCE:mutant phenotype:GO:0051724 NAD transmembrane transporter activity [PMID:16291748]
Mitochondrial NAD+ transporter; involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs; YIA6 has a paralog, YEA6, that arose from the whole genome duplication; human NAD+ transporter MCART1/SLC25A51 functionally complements the yia6 yea6 double null mutant, and yeast YIA6 reciprocally complements defects in MCART1/SLC25A51 null cells
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10545109]
EXISTENCE:direct assay:GO:0019153 protein-disulfide reductase (glutathione) activity [PMID:16002399]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16002399]
EXISTENCE:mutant phenotype:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:10545109]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:12881414]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16002399]
ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11001058]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:1396561]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9813082]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1396561]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:11001058]
EXISTENCE:mutant phenotype:GO:0005484 SNAP receptor activity [PMID:3312234]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:3312234]
Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
iron-sulfur cluster assembly protein CFD1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12970194]
EXISTENCE:direct assay:GO:0016226 iron-sulfur cluster assembly [PMID:17401378]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:26195633]
EXISTENCE:direct assay:GO:0051539 4 iron, 4 sulfur cluster binding [PMID:17401378]
EXISTENCE:direct assay:GO:1904564 Nbp35-Cfd1 ATPase complex [PMID:26195633]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:12970194]
Highly conserved iron-sulfur cluster binding protein; localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
Putative mitochondrial hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:9565610|PMID:10224048]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17452534]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9560389]
EXISTENCE:direct assay:GO:0046856 phosphatidylinositol dephosphorylation [PMID:10224048]
EXISTENCE:mutant phenotype:GO:0004439 phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity [PMID:9438131|PMID:9565610]
EXISTENCE:mutant phenotype:GO:0046856 phosphatidylinositol dephosphorylation [PMID:9438131|PMID:9560389]
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
hypothetical protein; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10764794]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:10764794]
EXISTENCE:genetic interaction:GO:0016071 mRNA metabolic process [PMID:10764794]
EXISTENCE:physical interaction:GO:0016071 mRNA metabolic process [PMID:10764794]
Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
ARS920; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:26966248]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10880470|PMID:19995966]
EXISTENCE:direct assay:GO:0033677 DNA/RNA helicase activity [PMID:26966248]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:15634678]
EXISTENCE:direct assay:GO:0060543 negative regulation of strand invasion [PMID:19136626]
EXISTENCE:direct assay:GO:0070336 flap-structured DNA binding [PMID:19181670]
EXISTENCE:genetic interaction:GO:0033567 DNA replication, Okazaki fragment processing [PMID:19181670]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:19995966]
EXISTENCE:mutant phenotype:GO:0007535 donor selection [PMID:27257873]
EXISTENCE:mutant phenotype:GO:0060543 negative regulation of strand invasion [PMID:19136626]
EXISTENCE:physical interaction:GO:0007535 donor selection [PMID:27257873]
3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer
EXISTENCE:direct assay:GO:0015629 actin cytoskeleton [PMID:14562095]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:28706108|PMID:29467252]
EXISTENCE:direct assay:GO:0030837 negative regulation of actin filament polymerization [PMID:29467252]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:29467252]
EXISTENCE:mutant phenotype:GO:0034642 mitochondrion migration along actin filament [PMID:19300474]
EXISTENCE:physical interaction:GO:0110131 Aim21-Tda2 complex [PMID:28706108|PMID:29467252]
Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p
ARS921; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9679141]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:30213914]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:9679141]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:23959800]
ER-associated chaperone involved in protein targeting; redirects mitochondrial membrane protein precursors to mitochondrial translocation system; required for peroxisomal protein import and involved in peroxisome assembly; facilitates import of Mim1p and Mim2p into the mitochondrial outer membrane; homologous to E. coli DnaJ
EXISTENCE:direct assay:GO:0000384 first spliceosomal transesterification activity [PMID:11287609]
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:15565172]
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:16314500]
EXISTENCE:direct assay:GO:0070274 RES complex [PMID:15565172]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:11287609]
EXISTENCE:mutant phenotype:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:11287609]
EXISTENCE:mutant phenotype:GO:0000384 first spliceosomal transesterification activity [PMID:11287609]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:15565172]
Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17967424]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17967424]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11914276]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:14622601|PMID:16824951]
EXISTENCE:direct assay:GO:0071933 Arp2/3 complex binding [PMID:11433303]
EXISTENCE:direct assay:GO:1990964 actin cytoskeleton-regulatory complex [PMID:10594004|PMID:17151356]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:16824951|PMID:11433303|PMID:17151356]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:18177206]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8978817]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:8756649]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:8756649]
EXISTENCE:physical interaction:GO:1990964 actin cytoskeleton-regulatory complex [PMID:17151356]
Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; regulates late stages of endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26116408]
EXISTENCE:direct assay:GO:0050295 steryl-beta-glucosidase activity [PMID:26116408]
EXISTENCE:mutant phenotype:GO:1904462 ergosteryl 3-beta-D-glucoside catabolic process [PMID:26116408]
Steryl-beta-glucosidase with broad specificity for aglycones; has a role in ergosteryl-beta-glucoside catabolism; required for normal vacuolar morphology; has similarity to the C. neoformans ergosteryl-beta-glucosidase EGCrP2; localizes to the cytosol
EXISTENCE:curator inference:GO:0006270 DNA replication initiation [PMID:3888995]
EXISTENCE:curator inference:GO:0006273 lagging strand elongation [PMID:3888995]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:20404922]
EXISTENCE:direct assay:GO:0003896 DNA primase activity [PMID:3888995|PMID:2644256|PMID:3061469]
EXISTENCE:direct assay:GO:0005658 alpha DNA polymerase:primase complex [PMID:3888995|PMID:22593576]
EXISTENCE:direct assay:GO:0006269 DNA replication, synthesis of RNA primer [PMID:2644256]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:genetic interaction:GO:0003896 DNA primase activity [PMID:8436268]
EXISTENCE:mutant phenotype:GO:0003896 DNA primase activity [PMID:8436268]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:2023935]
Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9799242]
EXISTENCE:physical interaction:GO:0030620 U2 snRNA binding [PMID:8649387]
U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
EXISTENCE:direct assay:GO:0000444 MIS12/MIND type complex [PMID:14633972]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14657030|PMID:24402315]
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:12455957]
EXISTENCE:direct assay:GO:0007059 chromosome segregation [PMID:12455957]
EXISTENCE:genetic interaction:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:23861669]
Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation promotes interaction between outer and inner kinetochore proteins; kinetochore receptor for monopolin, via interaction with Csm1p; essential for both meiotic and mitotic chromosome segregation; MIND complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; phosphorylated by monopolin subunit, Hrr25p and Aurora kinase, Ipl1p; modified by sumoylation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10913188]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:17916559]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8065366]
EXISTENCE:mutant phenotype:GO:0031144 proteasome localization [PMID:21075847]
EXISTENCE:mutant phenotype:GO:0071630 nuclear protein quality control by the ubiquitin-proteasome system [PMID:21075847]
Protein required for localizing proteasomes to the nucleus; involved in cotranslational protein degradation; mediates interaction between nuclear import factor Srp1p and the proteasome; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; involved in ubiquitin-mediated protein degradation
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9271392]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:26112308]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:9271392]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:26112308]
Specific assembly chaperone for ribosomal protein Rpl10p; co-translationally associates with nascent Rpl10p, preventing aggregation; involved in biogenesis of the ribosomal large subunit; contains multiple WD repeats; interacts genetically and physically with Qsr1p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10392447]
Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT3, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
EXISTENCE:direct assay:GO:0044695 Dsc E3 ubiquitin ligase complex [PMID:29355480]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:29355480]
Component of Dsc E3 ligase complex in vacuolar membranes; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:19095620]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:19095620|PMID:9620854]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9620854]
Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
hypothetical protein; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:8665859]
EXISTENCE:direct assay:GO:0089713 Cbf1-Met4-Met28 complex [PMID:8665859|PMID:9171357]
EXISTENCE:direct assay:GO:2000679 positive regulation of transcription regulatory region DNA binding [PMID:9171357]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:8665859]
EXISTENCE:mutant phenotype:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:8665859]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:8665859]
bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:18287073]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18070921]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9372930]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18287073]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18287073|PMID:18070921]
Basic leucine zipper (bZIP) iron-sensing transcription factor; senses high-iron conditions via two Fe/S clusters bound to its activator domain; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:20619652]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11027318]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:19799621]
EXISTENCE:direct assay:GO:0030447 filamentous growth [PMID:12072450]
EXISTENCE:mutant phenotype:GO:0000128 flocculation [PMID:19160455]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916|PMID:19087208|PMID:8710886]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8710886]
EXISTENCE:mutant phenotype:GO:0043709 cell adhesion involved in single-species biofilm formation [PMID:11157168]
EXISTENCE:mutant phenotype:GO:0044011 single-species biofilm formation on inanimate substrate [PMID:11157168|PMID:19160455]
EXISTENCE:mutant phenotype:GO:0090606 single-species surface biofilm formation [PMID:12024013|PMID:19160455]
EXISTENCE:mutant phenotype:GO:0098609 cell-cell adhesion [PMID:19160455|PMID:21875945]
GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal and invasive growth, flocculation, and biofilm formation; major determinant of colony morphology; QTL that controls chronological life span; carries intragenic tandem repeats that are expanded in different strains; required for formation of fibrous interconnections between cells; role in co-flocculation with other yeast species; cleaved and shed from cells, contributing to their surface properties
Upstream open reading frame (uORF) in 5' untranslated region of FLO11 gene, regulate translation
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:19805129]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19805129]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19805129]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19805129]
Long intergenic regulatory ncRNA; has a key role in regulating transcription of the nearby protein-coding ORF FLO11; initiated far upstream from FLO11 and transcribed across much of the large promoter of FLO11, repressing FLO11 transcription in cis
hypothetical protein; mRNA identified as translated by ribosome profiling data; SWAT-GFP fusion protein localizes to the endoplasmic reticulum
Intergenic regulatory ncRNA; one of two long ncRNAs that play key roles in regulating transcription of the nearby protein-coding ORF FLO11; PWR1 promotes FLO11 transcription by interfering with ncRNA ICR1, which is transcribed across the FLO11 promoter
EXISTENCE:curator inference:GO:0005739 mitochondrion [PMID:2443348]
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:11773622]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11773622]
EXISTENCE:direct assay:GO:1990904 ribonucleoprotein complex [PMID:12924947]
EXISTENCE:mutant phenotype:GO:0000963 mitochondrial RNA processing [PMID:2443348]
Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication
signal peptidase complex catalytic subunit SEC11
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11058593]
EXISTENCE:direct assay:GO:0005787 signal peptidase complex [PMID:8910564|PMID:1846444]
EXISTENCE:direct assay:GO:0006465 signal peptide processing [PMID:10206957]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:10206957|PMID:3283143]
EXISTENCE:mutant phenotype:GO:0008233 peptidase activity [PMID:10206957]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:3283143]
18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:21515579]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:21515579]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:21515579]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:7899074]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:21515579]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:21515579]
EXISTENCE:mutant phenotype:GO:1901714 positive regulation of urea catabolic process [PMID:2406136]
EXISTENCE:mutant phenotype:GO:1901717 positive regulation of gamma-aminobutyric acid catabolic process [PMID:2406136]
EXISTENCE:physical interaction:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:10906145]
Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14562095|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:24942160]
EXISTENCE:direct assay:GO:1990524 INA complex [PMID:24942160]
EXISTENCE:genetic interaction:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:24942160]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:24942160]
EXISTENCE:physical interaction:GO:1990524 INA complex [PMID:24942160]
F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15797380]
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:12609981|PMID:12574115]
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:22940250]
EXISTENCE:direct assay:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:15797379]
EXISTENCE:direct assay:GO:1990948 ubiquitin ligase inhibitor activity [PMID:15797379]
EXISTENCE:genetic interaction:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:15797379]
EXISTENCE:genetic interaction:GO:1905785 negative regulation of anaphase-promoting complex-dependent catabolic process [PMID:15797380]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:15797379]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0030071 regulation of mitotic metaphase/anaphase transition [PMID:12609981]
EXISTENCE:mutant phenotype:GO:1902426 deactivation of mitotic spindle assembly checkpoint [PMID:22940250]
EXISTENCE:mutant phenotype:GO:1902499 positive regulation of protein autoubiquitination [PMID:22940250]
EXISTENCE:physical interaction:GO:0005680 anaphase-promoting complex [PMID:15797379]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:1334559]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19797078|PMID:19797079]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19797078|PMID:19797079|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:1990275 preribosome binding [PMID:19797078]
EXISTENCE:genetic interaction:GO:0000027 ribosomal large subunit assembly [PMID:19797078]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:19797079]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:10464190]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:19114459|PMID:19797078]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:19797079]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:10464190]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8896280]
EXISTENCE:mutant phenotype:GO:2000786 positive regulation of autophagosome assembly [PMID:26125457]
Dual specificity protein phosphatase; regulates growth, sporulation, and glycogen accumulation in a cAMP-dependent protein kinase cascade dependent manner; mutants are defective in 60S ribosome assembly; positively regulates pre-autophagosomal structure (PAS) formation upon nitrogen starvation or rapamycin treatment
EXISTENCE:mutant phenotype:GO:0004038 allantoinase activity [PMID:4604238|PMID:1803816]
EXISTENCE:mutant phenotype:GO:0009442 allantoin assimilation pathway [PMID:1803816|PMID:4604238]
Allantoinase; converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
EXISTENCE:mutant phenotype:GO:0000256 allantoin catabolic process [PMID:385448]
EXISTENCE:mutant phenotype:GO:0005274 allantoin:proton symporter activity [PMID:385448]
EXISTENCE:mutant phenotype:GO:0015720 allantoin transport [PMID:3549700|PMID:385448]
Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
EXISTENCE:direct assay:GO:0004037 allantoicase activity [PMID:3915539]
EXISTENCE:mutant phenotype:GO:0000256 allantoin catabolic process [PMID:1916277]
Allantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
ARS922; Autonomously Replicating Sequence
EXISTENCE:expression pattern:GO:0006807 nitrogen compound metabolic process [PMID:1916277]
hypothetical protein; expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain
EXISTENCE:direct assay:GO:0000256 allantoin catabolic process [PMID:8462696]
EXISTENCE:direct assay:GO:0004474 malate synthase activity [PMID:8462696]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27678487]
EXISTENCE:mutant phenotype:GO:0004474 malate synthase activity [PMID:23642236]
Malate synthase; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA; recycles glyoxylate generated during allantoin degradation; SWAT-GFP and mCherry fusion proteins localize to the cytosol; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
EXISTENCE:curator inference:GO:0000256 allantoin catabolic process [PMID:3915539]
EXISTENCE:direct assay:GO:0050385 ureidoglycolate lyase activity [PMID:5965980]
EXISTENCE:mutant phenotype:GO:0050385 ureidoglycolate lyase activity [PMID:3915539]
Ureidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to 'ureidoglycolate hydrolase' but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19061897]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11007476]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11063674]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9927444]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11557770|PMID:29627385|PMID:11509659]
EXISTENCE:mutant phenotype:GO:0048255 mRNA stabilization [PMID:15220333]
ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication
saccharopine dehydrogenase (NAD+, L-lysine-forming)
EXISTENCE:direct assay:GO:0004754 saccharopine dehydrogenase (NAD+, L-lysine-forming) activity [PMID:17002315]
EXISTENCE:mutant phenotype:GO:0009085 lysine biosynthetic process [PMID:17247984]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:35563734]
Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; contains mRNA binding activity; required for targeting of Pls1p to the peroxisomal matrix in the absence of lysine
Putative cytoplasmic short-chain dehydrogenase/reductase
sepiapterin reductase family protein IRC24
EXISTENCE:direct assay:GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor [PMID:11796169]
Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:19755417]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:22659048]
EXISTENCE:direct assay:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
EXISTENCE:mutant phenotype:GO:0004602 glutathione peroxidase activity [PMID:10480913]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11445588]
EXISTENCE:mutant phenotype:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
Glutathione peroxidase; functions as hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce redox signal to Yap1p transcription factor; glutathione peroxidase activity is neuroprotective in models of Huntington's disease; HYR1 has a paralog, GPX1, that arose from the whole genome duplication
bifunctional glutathione transferase/peroxidase
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:9792709]
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:16709151]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9792709|PMID:26928762]
EXISTENCE:direct assay:GO:0006749 glutathione metabolic process [PMID:9792709]
EXISTENCE:mutant phenotype:GO:0010731 protein glutathionylation [PMID:27708136]
ER associated glutathione S-transferase; capable of homodimerization; glutathione transferase for Yvc1p vacuolar cation channel; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:16087741]
Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole
hypothetical protein; YIR042C is a non-essential gene
Blocked reading frame; may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
TEL09R; Telomeric region on the right arm of Chromosome IX; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats