Saccharomyces cerevisiae S288C chromosome VIII, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006934.\n\nOn Apr 26, 2011 this sequence version replaced NC_001140.5.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL08L; Telomeric region on the left arm of Chromosome VIII; composed of an X element core sequence, an X element combinatorial repeat, a short Y' element, and a short terminal stretch of telomeric repeats
hypothetical protein; potential Cdc28p substrate
ARS801; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
hypothetical protein; identified by expression profiling and mass spectrometry; localizes to the peroxisome in a Pex5p-dependent manner in oleate
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12051917]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
ARS802; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0015343 siderophore-iron transmembrane transporter activity [PMID:10816729]
EXISTENCE:direct assay:GO:0015891 siderophore transport [PMID:10816729]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10816729]
EXISTENCE:mutant phenotype:GO:0009237 siderophore metabolic process [PMID:10816729]
EXISTENCE:mutant phenotype:GO:0015343 siderophore-iron transmembrane transporter activity [PMID:10748025|PMID:10816729]
EXISTENCE:mutant phenotype:GO:0015891 siderophore transport [PMID:10816729]
Transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
Peroxisomal hypothetical protein; last 10 C-terminal amino acids contains a novel PTS1 motif, both necessary and sufficient for Pex5p-dependent peroxisomal targeting; also localizes to the nucleus; PXP3 is not an essential gene
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:12101299]
Putative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
hypothetical protein; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:31945465]
Endoplasmic reticulum protein; member of DUP380 subfamily of conserved, often subtelomerically-encoded proteins; may contribute to maintenance of ER and nuclear morphology; null mutants have distorted nuclear envelope shape, and discontinuous, misshapen perinuclear ER
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17344478]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15719020]
EXISTENCE:direct assay:GO:0031410 cytoplasmic vesicle [PMID:10748025]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0015343 siderophore-iron transmembrane transporter activity [PMID:10802175]
EXISTENCE:mutant phenotype:GO:0015891 siderophore transport [PMID:10802175]
ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:22522802]
EXISTENCE:mutant phenotype:GO:0018026 peptidyl-lysine monomethylation [PMID:20510667|PMID:22522802|PMID:24517342]
Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:9092668]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10521416]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2685564|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:10521416]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:6348045]
Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:genetic interaction:GO:0015191 L-methionine transmembrane transporter activity [PMID:8893857]
EXISTENCE:genetic interaction:GO:1903692 methionine import across plasma membrane [PMID:8893857]
EXISTENCE:mutant phenotype:GO:0015191 L-methionine transmembrane transporter activity [PMID:8893857]
EXISTENCE:mutant phenotype:GO:1903692 methionine import across plasma membrane [PMID:8893857]
Low affinity methionine permease; similar to Mup1p
putative ATP-binding cassette multidrug transporter VMR1
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20846144|PMID:23658021]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:genetic interaction:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:20846144]
EXISTENCE:mutant phenotype:GO:0010038 response to metal ion [PMID:20846144]
EXISTENCE:mutant phenotype:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:20846144]
Vacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000932 P-body [PMID:16782896|PMID:23222640]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:2121740|PMID:2823109]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16782896]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:22284680]
EXISTENCE:direct assay:GO:0031370 eukaryotic initiation factor 4G binding [PMID:22284680]
EXISTENCE:mutant phenotype:GO:0032055 negative regulation of translation in response to stress [PMID:16782896]
Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22893726]
Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB during assembly of large subunit; binds Domain I of 25S, 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; RPL8A has paralog RPL8B from whole genome duplication; RPL8A or RPL8B ribosomes not functionally interchangeable; proportions of Rpl8ap, Rpl8bp in 80S ribosome differ in glucose and glycerol; homologous to mammalian ribosomal protein L7A, no bacterial homolog
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397]
EXISTENCE:mutant phenotype:GO:0004370 glycerol kinase activity [PMID:8358828]
EXISTENCE:mutant phenotype:GO:0006071 glycerol metabolic process [PMID:8358828]
Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:11959998]
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:16699523]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11959998]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:11959998]
EXISTENCE:mutant phenotype:GO:0048193 Golgi vesicle transport [PMID:9755865]
v-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28
EXISTENCE:direct assay:GO:0000502 proteasome complex [PMID:12408819]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0043248 proteasome assembly [PMID:20620957]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:20620957]
EXISTENCE:mutant phenotype:GO:0042030 ATPase inhibitor activity [PMID:23995839]
Scaffold protein; assists in association of proteasome core particle with regulatory particle; inhibitor of proteasome in vivo and in vitro; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10196219]
EXISTENCE:genetic interaction:GO:0009408 response to heat [PMID:11589572]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:10196219]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:10196219]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:10196219]
Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
alkaline-responsive transcriptional regulator RIM101
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12509465]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:16024810]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20382759]
EXISTENCE:genetic interaction:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:16024810]
EXISTENCE:genetic interaction:GO:0009272 fungal-type cell wall biogenesis [PMID:16524906]
EXISTENCE:genetic interaction:GO:0010973 positive regulation of division septum assembly [PMID:19633265]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:16024810]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:16524906]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:8417990]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8417990]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8367297]
EXISTENCE:mutant phenotype:GO:0071454 cellular response to anoxia [PMID:20402793]
EXISTENCE:mutant phenotype:GO:0071469 cellular response to alkaline pH [PMID:11050096]
Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression; involved in cell wall assembly; plays role in lager yeast flocculation under brewing conditions; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
hypothetical protein; transcriptionally regulated by Upc2p via an upstream sterol response element; SWAT-GFP fusion protein localizes to the cell periphery, while mCherry fusion localizes to both the cell periphery and vacuole; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:23437238]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2188093|PMID:22932476]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:1459453]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1901413]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:23437238]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:17013386]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:17013386]
EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF complex [PMID:8159677]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:23437238]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1339306]
EXISTENCE:physical interaction:GO:0006289 nucleotide-excision repair [PMID:17013386]
Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:10806425]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:11713679]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:11713679]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:10806425]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:11713679]
Putative RNA-binding protein; involved in regulation of early and middle sporulation genes; forms amyloid-like aggregates under starvation that are active in translational repression
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:direct assay:GO:1990130 GATOR1 complex [PMID:21900499]
EXISTENCE:genetic interaction:GO:0010508 positive regulation of autophagy [PMID:25046117]
EXISTENCE:genetic interaction:GO:0051058 negative regulation of small GTPase mediated signal transduction [PMID:23716719]
EXISTENCE:genetic interaction:GO:1904262 negative regulation of TORC1 signaling [PMID:23716719|PMID:25046117]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:25046117]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:18069899]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:19521502|PMID:23716719|PMID:25046117]
EXISTENCE:mutant phenotype:GO:2000785 regulation of autophagosome assembly [PMID:21900499]
Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a coatomer-related complex that associates dynamically with the vacuole; Npr3p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and leading to inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; null mutant has meiotic defects; human NPRL3 homolog
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9039264]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23246437]
EXISTENCE:direct assay:GO:0045027 DNA end binding [PMID:9039264]
EXISTENCE:genetic interaction:GO:0042138 meiotic DNA double-strand break formation [PMID:2190690]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:8207053]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:2687110]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:2190690]
Meiosis-specific protein required for meiotic recombination; makes DNA double-strand breaks (DSB) during meiotic recombination, homolog pairing, and synaptonemal complex formation; role in meiotic DSB formation involves a topo-II-like transesterase mechanism; forms monotetrameric complex; covalently linked to DSBs through a 5'-phosphodiester bond between a catalytic tyrosine (Y135) and the DSB 5'- end; sequence similarity to a topo VI subunit from archaea, fission yeast rec12 and human SPO11
ARS805; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:19300474]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15192221|PMID:15455074|PMID:27663510]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15192221]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15455074]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15192221]
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:15455074]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:27480805]
EXISTENCE:direct assay:GO:0071072 negative regulation of phospholipid biosynthetic process [PMID:27743455]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:1985968]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:1985968]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16582425]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12871953]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:11454208]
Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:26658609]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:26658609]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:17003107]
hypothetical protein; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
4a-hydroxytetrahydrobiopterin dehydratase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Putative 4a-hydroxytetrahydrobiopterin dehydratase; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17218313]
EXISTENCE:direct assay:GO:0015489 putrescine transmembrane transporter activity [PMID:17218313]
EXISTENCE:direct assay:GO:0015606 spermidine transmembrane transporter activity [PMID:17218313]
EXISTENCE:direct assay:GO:0015847 putrescine transport [PMID:17218313]
EXISTENCE:direct assay:GO:0015848 spermidine transport [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:8335627]
EXISTENCE:mutant phenotype:GO:0015204 urea transmembrane transporter activity [PMID:8335627]
EXISTENCE:mutant phenotype:GO:0015489 putrescine transmembrane transporter activity [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015606 spermidine transmembrane transporter activity [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015840 urea transport [PMID:8335627]
EXISTENCE:mutant phenotype:GO:0015847 putrescine transport [PMID:17218313]
EXISTENCE:mutant phenotype:GO:0015848 spermidine transport [PMID:17218313]
Plasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:3533916]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:3533916]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
hypothetical protein; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
hypothetical protein; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
UTP--glucose-1-phosphate uridylyltransferase
Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
ribose phosphate diphosphokinase subunit PRS3
EXISTENCE:direct assay:GO:0002189 ribose phosphate diphosphokinase complex [PMID:15280369]
EXISTENCE:genetic interaction:GO:0004749 ribose phosphate diphosphokinase activity [PMID:15280369]
EXISTENCE:genetic interaction:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:15280369]
EXISTENCE:mutant phenotype:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:10212224]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:15470112]
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:9497340]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:16564012]
EXISTENCE:mutant phenotype:GO:0045471 response to ethanol [PMID:19416103]
hypothetical protein required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9372930]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:9372930]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9372930]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
Basic leucine zipper (bZIP) transcription factor
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
YHLWTy4-1; Ty4 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006821 chloride transport [PMID:18378800]
EXISTENCE:mutant phenotype:GO:0140299 small molecule sensor activity [PMID:18378800]
EXISTENCE:mutant phenotype:GO:2001225 regulation of chloride transport [PMID:18378800]
hypothetical protein; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
mitogen-activated protein kinase kinase kinase kinase STE20
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9003780]
EXISTENCE:direct assay:GO:0000165 MAPK cascade [PMID:7608157]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19793923|PMID:15652479]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:7608157]
EXISTENCE:direct assay:GO:0008349 MAP kinase kinase kinase kinase activity [PMID:7608157]
EXISTENCE:direct assay:GO:0019899 enzyme binding [PMID:31395335]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9003780]
EXISTENCE:direct assay:GO:0044025 histone H2BS14 kinase activity [PMID:15652479]
EXISTENCE:genetic interaction:GO:0000011 vacuole inheritance [PMID:19218422]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:1464311]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:genetic interaction:GO:0007121 bipolar cellular bud site selection [PMID:10866679]
EXISTENCE:genetic interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:10970855]
EXISTENCE:genetic interaction:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:genetic interaction:GO:0034063 stress granule assembly [PMID:20513766]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:19218422]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:11452010]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:1464311]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:8259520]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:8001818]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8259520]
EXISTENCE:mutant phenotype:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:10970855]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:20513766]
EXISTENCE:mutant phenotype:GO:0035376 sterol import [PMID:19793923]
EXISTENCE:mutant phenotype:GO:0043065 positive regulation of apoptotic process [PMID:15652479]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9428767]
Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:21372173]
EXISTENCE:direct assay:GO:0097196 Shu complex [PMID:23575680]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:15654096]
EXISTENCE:genetic interaction:GO:0043007 maintenance of rDNA [PMID:21372173]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0000730 DNA recombinase assembly [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0035861 site of double-strand break [PMID:23575680]
EXISTENCE:physical interaction:GO:0097196 Shu complex [PMID:15654096]
Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments
hypothetical protein; conserved among S. cerevisiae strains; YHL005C is not an essential gene
mitochondrial 37S ribosomal protein MRP4
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276|PMID:14562095|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0046513 ceramide biosynthetic process [PMID:15692566]
EXISTENCE:direct assay:GO:0050291 sphingosine N-acyltransferase activity [PMID:15692566]
EXISTENCE:direct assay:GO:0061576 acyl-CoA ceramide synthase complex [PMID:15692566]
EXISTENCE:mutant phenotype:GO:0046513 ceramide biosynthetic process [PMID:11694577]
EXISTENCE:mutant phenotype:GO:0050291 sphingosine N-acyltransferase activity [PMID:11387200|PMID:11694577]
Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12055639]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:31740006]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12055639|PMID:20150893]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12055639]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:31740006]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:12055639]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0006623 protein targeting to vacuole [PMID:12055639]
EXISTENCE:physical interaction:GO:0033565 ESCRT-0 complex [PMID:12055639]
Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:29788267]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:29788267]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:29788267]
Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
oxysterol-binding protein related protein OSH7
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16096648]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:20008566]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:29487131]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0016125 sterol metabolic process [PMID:16096648]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:16096648]
Oxysterol-binding phosphatidylserine transferase; part of family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH7 has a paralog, OSH6, that arose from the whole genome duplication
ubiquinol--cytochrome-c reductase subunit 10
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:8175712]
EXISTENCE:genetic interaction:GO:0009060 aerobic respiration [PMID:8175712]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:8175712]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:8175712]
Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11158296]
EXISTENCE:mutant phenotype:GO:0015228 coenzyme A transmembrane transporter activity [PMID:11158296]
EXISTENCE:mutant phenotype:GO:0015880 coenzyme A transport [PMID:11158296]
Mitochondrial carrier protein; involved in the accumulation of CoA in the mitochondrial matrix; homolog of human Graves disease protein SLC25A16, which complements yeast null mutant; does not encode an isozyme of Leu4p, as first hypothesized
tRNA threonylcarbamoyladenosine dehydratase
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:mutant phenotype:GO:0061503 tRNA threonylcarbamoyladenosine dehydratase [PMID:23242255]
EXISTENCE:mutant phenotype:GO:0061504 cyclic threonylcarbamoyladenosine biosynthetic process [PMID:23242255]
tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication
Nem1-Spo7 phosphatase catalytic subunit NEM1
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:15889145|PMID:25359770]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:32349126]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:26275961]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9822591]
EXISTENCE:direct assay:GO:1903740 positive regulation of phosphatidate phosphatase activity [PMID:25359770]
EXISTENCE:genetic interaction:GO:0071072 negative regulation of phospholipid biosynthetic process [PMID:15889145]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:9822591]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:32349126]
EXISTENCE:mutant phenotype:GO:0046889 positive regulation of lipid biosynthetic process [PMID:32349126]
EXISTENCE:mutant phenotype:GO:0071071 regulation of phospholipid biosynthetic process [PMID:30201607]
EXISTENCE:mutant phenotype:GO:0140042 lipid droplet formation [PMID:32349126]
EXISTENCE:physical interaction:GO:0071595 Nem1-Spo7 phosphatase complex [PMID:9822591]
Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear/ER membrane association and activation of Pah1p, a phosphatidate phosphatase involved in the production of diacylglycerol DAG for lipid droplet biogenesis; regulates nuclear growth by controlling phospholipid biosynthesis; required for normal nuclear envelope morphology and sporulation; phosphatase activity of the Nem1p-Spo7p complex is inhibited by Ice2p; homolog of the human protein Dullard
guanine nucleotide-binding protein subunit alpha
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11394869]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:16839886]
EXISTENCE:direct assay:GO:0005834 heterotrimeric G-protein complex [PMID:11394869]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8756677]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:2107073]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:19386762]
EXISTENCE:mutant phenotype:GO:0000743 nuclear migration involved in conjugation with cellular fusion [PMID:19386762]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3113739]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12556475|PMID:2105453]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:1900495]
EXISTENCE:mutant phenotype:GO:0005834 heterotrimeric G-protein complex [PMID:2105453]
EXISTENCE:mutant phenotype:GO:0006109 regulation of carbohydrate metabolic process [PMID:34214075]
EXISTENCE:mutant phenotype:GO:0048017 inositol lipid-mediated signaling [PMID:16839886]
EXISTENCE:mutant phenotype:GO:0071701 regulation of MAPK export from nucleus [PMID:12556475]
EXISTENCE:physical interaction:GO:0005834 heterotrimeric G-protein complex [PMID:11394869]
EXISTENCE:physical interaction:GO:0031681 G-protein beta-subunit binding [PMID:8417317]
Subunit of G protein involved in pheromone response; GTP-binding alpha subunit of heterotrimeric G protein; negatively regulates mating pathway by sequestering G(beta)gamma and triggering an adaptive response; activates Vps34p at endosome; protein abundance increases in response to DNA replication stress; mutations in human homolog GNAS associated with McCune-Albright syndrome
EXISTENCE:direct assay:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:9889188|PMID:9822593]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9495346]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:12138093]
EXISTENCE:direct assay:GO:0140318 protein transporter activity [PMID:9495346]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9430585]
EXISTENCE:mutant phenotype:GO:0140318 protein transporter activity [PMID:9430585]
Essential protein of the mitochondrial intermembrane space; forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
ARS806; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11212916]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10648791|PMID:19906648]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21127045]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21127045]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10648791|PMID:19906648]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10648791]
Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15951236|PMID:26928762|PMID:11914276]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:105731]
EXISTENCE:direct assay:GO:0008398 sterol 14-demethylase activity [PMID:9087488|PMID:105731]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:200835]
Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptionally down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functionally complements the lethality of the erg11 null mutation
hypothetical protein; identified by expression profiling and mass spectrometry; SWAT-GFP fusion protein localizes to the nucleus
EXISTENCE:direct assay:GO:0004784 superoxide dismutase activity [PMID:15851472|PMID:238997]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:238997]
EXISTENCE:mutant phenotype:GO:0072593 reactive oxygen species metabolic process [PMID:3520557]
Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21441304|PMID:10684247]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:21441304]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:21441304]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:21441304]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:21441304]
Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:genetic interaction:GO:0004828 serine-tRNA ligase activity [PMID:11747308]
EXISTENCE:genetic interaction:GO:0070158 mitochondrial seryl-tRNA aminoacylation [PMID:11747308]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:11747308]
Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth
EXISTENCE:mutant phenotype:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:mutant phenotype:GO:0140312 cargo adaptor activity [PMID:9700157]
EXISTENCE:physical interaction:GO:0005768 endosome [PMID:9700157]
EXISTENCE:physical interaction:GO:0030904 retromer complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0030906 retromer, cargo-selective complex [PMID:9700157]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:9700157]
EXISTENCE:physical interaction:GO:0140312 cargo adaptor activity [PMID:9700157]
Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
peptide alpha-N-acetyltransferase complex A subunit ARD1
EXISTENCE:direct assay:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:2551674]
EXISTENCE:direct assay:GO:0006474 N-terminal protein amino acid acetylation [PMID:2551674]
EXISTENCE:direct assay:GO:0031415 NatA complex [PMID:14517307]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:2551674]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:2551674]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:17652096]
EXISTENCE:mutant phenotype:GO:0061606 N-terminal protein amino acid propionylation [PMID:23043182]
Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:15620645]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12101125]
EXISTENCE:direct assay:GO:0072687 meiotic spindle [PMID:17493939]
EXISTENCE:mutant phenotype:GO:0045876 positive regulation of sister chromatid cohesion [PMID:12101125]
EXISTENCE:mutant phenotype:GO:0051177 meiotic sister chromatid cohesion [PMID:15620644|PMID:15620645]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:16357219]
Meiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
ARS807; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:28193845]
EXISTENCE:genetic interaction:GO:0006417 regulation of translation [PMID:28193845]
EXISTENCE:genetic interaction:GO:0010609 mRNA localization resulting in post-transcriptional regulation of gene expression [PMID:28193845]
EXISTENCE:genetic interaction:GO:0016071 mRNA metabolic process [PMID:28193845]
mRNA-binding protein; interacts with the UBA domain of mRNA export factor Mex67p; participates in the export of Msn2/4 stress-dependent mRNAs; partially redundant function with paralog PES4 in the steady-state expression, translational timing and localization of a subset of Ndt80-dependent mRNAs that are protected until translated near the end of meiosis II; redundant role with PES4 in sporulation; shuttles between the nucleus and cytoplasm, localizes to stress granules and the prospore membrane
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:12388763]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:19158382]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:19158382]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:12388763]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:12388763]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:19158382]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:19158382]
Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
Non-essential mitochondrial hypothetical protein; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
EXISTENCE:direct assay:GO:0004056 argininosuccinate lyase activity [PMID:4578972]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:205532]
EXISTENCE:direct assay:GO:0042450 arginine biosynthetic process via ornithine [PMID:4578972]
Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9605503]
EXISTENCE:direct assay:GO:0004816 asparagine-tRNA ligase activity [PMID:9605503]
EXISTENCE:direct assay:GO:0006421 asparaginyl-tRNA aminoacylation [PMID:9605503]
Cytosolic asparaginyl-tRNA synthetase; required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
EXISTENCE:direct assay:GO:0004827 proline-tRNA ligase activity [PMID:16864571]
EXISTENCE:direct assay:GO:0006433 prolyl-tRNA aminoacylation [PMID:16864571]
Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:3533916]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:9271380]
EXISTENCE:mutant phenotype:GO:0000479 endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9271380]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
hypothetical protein; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
hypothetical protein; YHR022C is not an essential gene
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9732290]
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:9442111]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9732290]
EXISTENCE:direct assay:GO:1904498 protein localization to mitotic actomyosin contractile ring [PMID:15210731]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:9732290]
EXISTENCE:mutant phenotype:GO:0031671 primary cell septum biogenesis [PMID:21173112]
EXISTENCE:mutant phenotype:GO:1902404 mitotic actomyosin contractile ring contraction [PMID:21173112|PMID:9864366|PMID:16148042]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:21173112|PMID:9732290]
EXISTENCE:mutant phenotype:GO:1904498 protein localization to mitotic actomyosin contractile ring [PMID:23468521]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:21173112|PMID:23468521|PMID:11082046]
EXISTENCE:physical interaction:GO:0016460 myosin II complex [PMID:15210731]
EXISTENCE:physical interaction:GO:0032027 myosin light chain binding [PMID:15210731]
EXISTENCE:physical interaction:GO:0032033 myosin II light chain binding [PMID:11082046]
Type II myosin heavy chain; required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
mitochondrial-processing protease subunit alpha
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:2905264]
EXISTENCE:direct assay:GO:0017087 mitochondrial processing peptidase complex [PMID:2905264]
EXISTENCE:mutant phenotype:GO:0006627 protein processing involved in protein targeting to mitochondrion [PMID:3061808]
Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
EXISTENCE:direct assay:GO:0004413 homoserine kinase activity [PMID:2165904]
EXISTENCE:direct assay:GO:0009092 homoserine metabolic process [PMID:2165904]
EXISTENCE:mutant phenotype:GO:0009088 threonine biosynthetic process [PMID:17247984]
Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway
H(+)-transporting V0 sector ATPase subunit c''
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15252052]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:9030535]
Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
proteasome regulatory particle base subunit RPN1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15210724|PMID:26928762]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:9741626]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:12198498]
Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:2647766|PMID:26928762]
EXISTENCE:direct assay:GO:0008239 dipeptidyl-peptidase activity [PMID:2647766]
Dipeptidyl aminopeptidase; synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:31323700]
hypothetical protein; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production
ARS809; Autonomously Replicating Sequence
mitogen-activated serine/threonine-protein kinase SLT2
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:20823268|PMID:24554767]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12361575|PMID:23924898|PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25598154]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12361575|PMID:23924898]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12361575|PMID:23924898]
EXISTENCE:direct assay:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16380504]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12361575]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:8386319|PMID:8386320]
EXISTENCE:genetic interaction:GO:0010973 positive regulation of division septum assembly [PMID:19633265|PMID:27400980]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:8386319]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:8386319]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:17302939]
EXISTENCE:mutant phenotype:GO:0010447 response to acidic pH [PMID:16087742]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16380504]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:27117666]
EXISTENCE:mutant phenotype:GO:0036498 IRE1-mediated unfolded protein response [PMID:18971375]
EXISTENCE:mutant phenotype:GO:0042306 regulation of protein import into nucleus [PMID:20219973]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:8386319]
EXISTENCE:mutant phenotype:GO:0060256 regulation of flocculation [PMID:31441971]
EXISTENCE:mutant phenotype:GO:0090364 regulation of proteasome assembly [PMID:27462806]
Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:12050116]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:17114583|PMID:26411679]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:30442759]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:26981397]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:15907372]
EXISTENCE:genetic interaction:GO:0071932 replication fork reversal [PMID:26411679]
EXISTENCE:mutant phenotype:GO:0005657 replication fork [PMID:17114583]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:10693764]
EXISTENCE:mutant phenotype:GO:0044806 G-quadruplex DNA unwinding [PMID:23657261]
EXISTENCE:mutant phenotype:GO:0097046 replication fork progression beyond termination site [PMID:20797631]
EXISTENCE:mutant phenotype:GO:0098781 ncRNA transcription [PMID:31391265]
DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; deletion or inactivation of helicase function suppresses chromosome instability (CIN) associated with centromere or kinetochore defects; structurally and functionally related to Pif1p
EXISTENCE:mutant phenotype:GO:0006556 S-adenosylmethionine biosynthetic process [PMID:18592490]
Member of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
hypothetical protein; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0097255 R2TP complex [PMID:15766533]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:15670595]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:18268103]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:15670595]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27053109]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:physical interaction:GO:0097255 R2TP complex [PMID:18268103]
Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:24947508]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:24947508]
Activator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15882446|PMID:26432634]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15882446|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15882446]
EXISTENCE:genetic interaction:GO:0055088 lipid homeostasis [PMID:26432634]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:15882446]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:26432634]
Essential nuclear envelope/ER integral membrane protein; interacts and functions with Apq12p and Brr6p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, mRNA nuclear export and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; identified as a dosage suppressor of a temperature sensitive mutation in the major karyopherin, CRM1; homologous to Brr6p
EXISTENCE:direct assay:GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity [PMID:4145181]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:3025596]
EXISTENCE:direct assay:GO:0006537 glutamate biosynthetic process [PMID:4145181]
EXISTENCE:mutant phenotype:GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity [PMID:387737]
EXISTENCE:mutant phenotype:GO:0010133 proline catabolic process to glutamate [PMID:387737]
Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12853640|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:12853640]
Mitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511544]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
H(+)-transporting V1 sector ATPase subunit G
EXISTENCE:direct assay:GO:0000221 vacuolar proton-transporting V-type ATPase, V1 domain [PMID:16774922]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922|PMID:26928762]
Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
EXISTENCE:direct assay:GO:0000492 box C/D snoRNP assembly [PMID:31537647]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591|PMID:14562095]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:31537647]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:31537647]
EXISTENCE:mutant phenotype:GO:0016074 sno(s)RNA metabolic process [PMID:12837249]
Essential protein required for the accumulation of box C/D snoRNA
EXISTENCE:direct assay:GO:0001094 TFIID-class transcription factor complex binding [PMID:1591782]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:1591782]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8187178]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:1591782]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:genetic interaction:GO:0019904 protein domain specific binding [PMID:2693207]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15831453]
EXISTENCE:mutant phenotype:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:18604275]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
EXISTENCE:direct assay:GO:0003958 NADPH-hemoprotein reductase activity [PMID:9087488]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0009055 electron transfer activity [PMID:11485306]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:9468503]
NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
EXISTENCE:direct assay:GO:0003850 2-deoxyglucose-6-phosphatase activity [PMID:8553694]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0006006 glucose metabolic process [PMID:8553694]
2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
hypothetical protein; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
inositol monophosphate 1-phosphatase INM1
EXISTENCE:direct assay:GO:0008934 inositol monophosphate 1-phosphatase activity [PMID:10096091]
EXISTENCE:direct assay:GO:0046855 inositol phosphate dephosphorylation [PMID:10844654]
EXISTENCE:genetic interaction:GO:0008934 inositol monophosphate 1-phosphatase activity [PMID:12593845]
EXISTENCE:genetic interaction:GO:0046855 inositol phosphate dephosphorylation [PMID:12593845]
Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
EXISTENCE:mutant phenotype:GO:0004177 aminopeptidase activity [PMID:8100228]
EXISTENCE:mutant phenotype:GO:0005977 glycogen metabolic process [PMID:8100228]
EXISTENCE:mutant phenotype:GO:0006508 proteolysis [PMID:8100228]
Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication
Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
EXISTENCE:direct assay:GO:0004622 lysophospholipase activity [PMID:32920715]
EXISTENCE:direct assay:GO:0016788 hydrolase activity, acting on ester bonds [PMID:32182256]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:31363875]
Lysophospholipase; hydrolyzes lysophosphatidylserine to release free fatty acid; involved in regulated cell death; localizes to both the nucleus and cytoplasm; contains a catalytic triad of Ser-His-Asp that is part of an alpha/beta hydrolase fold and a lipase motif (GXSXG); sequence similarity to Fsh2p and Fsh3p and the human candidate tumor suppressor and serine hydrolase, OVCA2
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:25713143]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:25713143]
EXISTENCE:direct assay:GO:0022890 inorganic cation transmembrane transporter activity [PMID:10574989]
EXISTENCE:genetic interaction:GO:0006824 cobalt ion transport [PMID:9115231]
EXISTENCE:genetic interaction:GO:0006877 cellular cobalt ion homeostasis [PMID:9115231]
EXISTENCE:mutant phenotype:GO:0006828 manganese ion transport [PMID:11602606]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:11602606]
Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11500370]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0030163 protein catabolic process [PMID:11500370]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:11500370]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14623999|PMID:21811236]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14623999]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:11500370]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:11500370]
Essential protein that interacts with proteasome components; has a potential role in proteasome substrate specificity; also copurifies with 66S pre-ribosomal particles
Novel ORF identified by enrichment-based proteomics
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:3902832]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:3308865|PMID:26928762]
EXISTENCE:direct assay:GO:0016209 antioxidant activity [PMID:8367458]
EXISTENCE:direct assay:GO:0019430 removal of superoxide radicals [PMID:8367458]
EXISTENCE:direct assay:GO:0046870 cadmium ion binding [PMID:3902832]
EXISTENCE:genetic interaction:GO:0019430 removal of superoxide radicals [PMID:8367458]
EXISTENCE:mutant phenotype:GO:0004784 superoxide dismutase activity [PMID:8367458]
EXISTENCE:mutant phenotype:GO:0010273 detoxification of copper ion [PMID:3536930]
EXISTENCE:mutant phenotype:GO:0046688 response to copper ion [PMID:6370791]
EXISTENCE:mutant phenotype:GO:0071585 detoxification of cadmium ion [PMID:3536930]
Metallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-1 has a paralog, CUP1-2, that arose from a segmental duplication
ARS810; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
hypothetical protein; partial duplicate of RSC30/YHR056C, truncated remnant of segmental duplication
Metallothionein; binds copper and mediates resistance to high concentrations of copper and cadmium; locus is variably amplified in different strains, with two copies, CUP1-1 and CUP1-2, in the genomic sequence reference strain S288C; CUP1-2 has a paralog, CUP1-1, that arose from a segmental duplication
ARS811; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:32188943]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:12183366|PMID:32188943]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:32188943]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11336698]
Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; null mutants are osmosensitive; RSC30 has a paralog, RSC3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:1761234]
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; seamless-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion localizes to both the endoplasmic reticulum and vacuole; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:9234719]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9234719]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:9234719]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9234719]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:28154081]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:28154081]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:28154081]
hypothetical protein; required for survival upon exposure to K1 killer toxin
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:1628805]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:7673216|PMID:8582630]
EXISTENCE:mutant phenotype:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:7673216]
EXISTENCE:physical interaction:GO:1990871 Vma12-Vma22 assembly complex [PMID:9660861]
Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9367979|PMID:9367980]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9367979|PMID:22842922|PMID:9367980]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9367979]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9367980]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:9367980]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:16371506]
EXISTENCE:genetic interaction:GO:0032488 Cdc42 protein signal transduction [PMID:9367979]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:14734533]
EXISTENCE:physical interaction:GO:0031106 septin ring organization [PMID:16371506]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:9367979|PMID:9367980]
Rho-family GTPase interacting protein and Cdc42p effector; required with Gic2p for polarity establishment and initiation of budding; involved in recruitment of septins to the presumptive bud site; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; gic1 gic2 double mutants are defective for polarizing Cdc42p at elevated temperature, so may function both up- and downstream of Cdc42p; regulates mitotic exit; relocalizes from bud neck to nucleus upon DNA replication stress
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:23700311]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9620854]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9353260|PMID:9620854]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9353260|PMID:9620854]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9353260|PMID:9620854]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9353260|PMID:9620854]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380|PMID:9353260]
EXISTENCE:physical interaction:GO:0005655 nucleolar ribonuclease P complex [PMID:9353260]
Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
EXISTENCE:curator inference:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
2-dehydropantoate 2-reductase; part of the pantothenic acid pathway, structurally homologous to E. coli panE
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10792726]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:16413483]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:11929994]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:15456889]
EXISTENCE:mutant phenotype:GO:0006452 translational frameshifting [PMID:16607023]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:11054575]
EXISTENCE:mutant phenotype:GO:0051083 'de novo' cotranslational protein folding [PMID:11274393]
Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:16449634]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:23153376]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:18975973|PMID:23153376]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16449634]
Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
rRNA-binding ribosome biosynthesis protein
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9611192]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:11864606]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11864607]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864607]
Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15387819|PMID:14562095|PMID:16823961]
EXISTENCE:direct assay:GO:0019171 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity [PMID:15387819]
Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
EXISTENCE:direct assay:GO:0008612 peptidyl-lysine modification to peptidyl-hypusine [PMID:7629166]
EXISTENCE:direct assay:GO:0034038 deoxyhypusine synthase activity [PMID:7629166]
Deoxyhypusine synthase; catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric; human homolog DHPS allows growth of yeast haploid dys1 null mutant after sporulation of heterozygous diploid
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:11586364]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172|PMID:8600032|PMID:9390555]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:9482746]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10611222]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791]
EXISTENCE:mutant phenotype:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10611222]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; mutations in the human ortholog, EXOSC2, cause SHRF (Short stature, Hearing loss, Retinitis pigmentosa and distinctive Facies) syndrome, an RNA exosomopathy
EXISTENCE:direct assay:GO:0002939 tRNA N1-guanine methylation [PMID:17652090]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17652090|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:17652090]
EXISTENCE:direct assay:GO:0009019 tRNA (guanine-N1-)-methyltransferase activity [PMID:17652090]
EXISTENCE:direct assay:GO:0070901 mitochondrial tRNA methylation [PMID:17652090]
EXISTENCE:mutant phenotype:GO:0002939 tRNA N1-guanine methylation [PMID:11226173]
EXISTENCE:mutant phenotype:GO:0009019 tRNA (guanine-N1-)-methyltransferase activity [PMID:11226173]
tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; localized to both cytoplasm and mitochondria, and modifies both cytoplasmic and mitochondrial tRNAs; mutations in human ortholog TRMT5 are associated with skeletal muscle respiratory chain deficiencies, and trm5 mutations analogous to disease mutations decrease respiration
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19218424]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:12101234]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:20417603]
EXISTENCE:mutant phenotype:GO:0031647 regulation of protein stability [PMID:12101234]
EXISTENCE:mutant phenotype:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:12101234]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:16611745]
Cyclin; interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000250 lanosterol synthase activity [PMID:12842197]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:11706015|PMID:24868093]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:12842197]
EXISTENCE:mutant phenotype:GO:0000250 lanosterol synthase activity [PMID:18842050]
Lanosterol synthase; an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis; human LSS functionally complements the lethality of the erg7 null mutation
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9843512]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9843512]
EXISTENCE:direct assay:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:mutant phenotype:GO:0000469 cleavage involved in rRNA processing [PMID:9843512]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9843512]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:9843512]
Subunit of box H/ACA snoRNP complex; required for pseudouridylation and processing of pre-18S rRNA
oxysterol-binding protein related protein OSH3
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11408574]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25420878]
EXISTENCE:direct assay:GO:0010922 positive regulation of phosphatase activity [PMID:21295699]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:20008566]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:20008566]
EXISTENCE:genetic interaction:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12054531]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:12054531]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:12054531|PMID:17082746]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:24213531]
Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding
ARS813; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity [PMID:12898714|PMID:12771147]
EXISTENCE:direct assay:GO:0004359 glutaminase activity [PMID:12771147]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12898714]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12898714]
EXISTENCE:direct assay:GO:0009435 NAD biosynthetic process [PMID:12771147|PMID:12898714]
EXISTENCE:mutant phenotype:GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity [PMID:12898714|PMID:12771147]
EXISTENCE:mutant phenotype:GO:0004359 glutaminase activity [PMID:12771147]
EXISTENCE:mutant phenotype:GO:0009435 NAD biosynthetic process [PMID:12771147|PMID:12898714]
Glutamine-dependent NAD(+) synthetase; essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
carboxylesterase-mitochondrial 37S ribosomal protein YmS2
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:9151978]
EXISTENCE:mutant phenotype:GO:0051722 protein C-terminal methylesterase activity [PMID:11060018]
EXISTENCE:mutant phenotype:GO:1990577 C-terminal protein demethylation [PMID:11060018]
Protein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:23940037]
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:12220683|PMID:23940037]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:19564484]
EXISTENCE:direct assay:GO:0006470 protein dephosphorylation [PMID:23940037]
Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16043493]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:9268361]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:16611983]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:23390378]
Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
High osmolarity-regulated gene of unknown function
bifunctional endoribonuclease/protein kinase IRE1
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:9323131]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8663458]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17035634]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8358794]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:17923530]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:18971375]
EXISTENCE:mutant phenotype:GO:0006020 inositol metabolic process [PMID:1625574]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:8358794|PMID:8513503]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:18971375|PMID:8898193]
EXISTENCE:mutant phenotype:GO:0034067 protein localization to Golgi apparatus [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:17923530]
Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:15579681|PMID:15620352]
EXISTENCE:mutant phenotype:GO:0000707 meiotic DNA recombinase assembly [PMID:15579681|PMID:15620352]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:9215889]
Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p
EXISTENCE:curator inference:GO:0032366 intracellular sterol transport [PMID:26001273]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:26001273]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:26001273]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:29339490]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:33255682]
Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:17704127]
EXISTENCE:direct assay:GO:0003725 double-stranded RNA binding [PMID:17704127]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23580640]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:24937447]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12837249|PMID:19046973]
EXISTENCE:direct assay:GO:1901917 regulation of exoribonuclease activity [PMID:24937447]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:17410208]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:18591258]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12972615]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:18614049]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:12972615]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:12972615]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:16652390]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:12972615]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:16973436|PMID:18591258]
EXISTENCE:mutant phenotype:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:12972615]
Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination
putative serine/threonine protein kinase KSP1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18417610|PMID:8676864]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18417610|PMID:22842922]
EXISTENCE:genetic interaction:GO:0016242 negative regulation of macroautophagy [PMID:22447937]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:18417610]
EXISTENCE:mutant phenotype:GO:0010570 regulation of filamentous growth [PMID:18417610]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:22447937]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:22043304]
EXISTENCE:mutant phenotype:GO:0043555 regulation of translation in response to stress [PMID:31455721]
EXISTENCE:mutant phenotype:GO:2000220 regulation of pseudohyphal growth [PMID:22043304|PMID:31455721]
EXISTENCE:physical interaction:GO:0031929 TOR signaling [PMID:22043304]
Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0001401 SAM complex [PMID:15067005|PMID:15205677]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:15067005|PMID:16407407|PMID:15205677]
EXISTENCE:mutant phenotype:GO:0001401 SAM complex [PMID:15205677]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:15326197|PMID:18039934|PMID:15067005]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:15067005|PMID:15205677|PMID:18039934]
Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
homeodomain family transcription factor STE12
EXISTENCE:direct assay:GO:0000747 conjugation with cellular fusion [PMID:3931921]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:2558054]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19159085]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:20852627]
EXISTENCE:direct assay:GO:1990526 Ste12p-Dig1p-Dig2p complex [PMID:16782869]
EXISTENCE:direct assay:GO:1990527 Tec1p-Ste12p-Dig1p complex [PMID:16782869]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16940175]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8259520|PMID:9055077]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7834739]
Transcription factor that is activated by a MAPK signaling cascade; activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:22421151]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:15528184]
EXISTENCE:direct assay:GO:0097344 Rix1 complex [PMID:14759368]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:15528184]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]
EXISTENCE:mutant phenotype:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:22421151]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:14759368]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:22421151]
Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23222640]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0006376 mRNA splice site selection [PMID:10072385]
EXISTENCE:mutant phenotype:GO:0048026 positive regulation of mRNA splicing, via spliceosome [PMID:10983980]
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
hypothetical protein; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:genetic interaction:GO:0016070 RNA metabolic process [PMID:15701634]
hypothetical protein involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog is SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress
rRNA-binding ribosome biosynthesis protein RPF1
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11864606]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:16581791]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11864606]
Protein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
H/ACA snoRNP pseudouridylase subunit GAR1
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:1531632|PMID:9843512]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:2898766]
EXISTENCE:physical interaction:GO:0034513 box H/ACA snoRNA binding [PMID:9556561]
Protein component of the H/ACA snoRNP pseudouridylase complex; involved in the modification and cleavage of the 18S pre-rRNA
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:17274630]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10805724|PMID:22932476]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0032777 Piccolo NuA4 histone acetyltransferase complex [PMID:12782659]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:17142463]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911]
Subunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; relocalizes to the cytosol in response to hypoxia; similar to human tumor suppressor ING1 and its isoforms ING4 and ING5
EXISTENCE:curator inference:GO:0070144 mitochondrial arginyl-tRNA aminoacylation [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:7607232]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7607232]
Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication
ARS814; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005355 glucose transmembrane transporter activity [PMID:12702270]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17507646|PMID:24124599]
EXISTENCE:mutant phenotype:GO:0015146 pentose transmembrane transporter activity [PMID:17180689]
High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10377429|PMID:24124599]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:7651133]
EXISTENCE:mutant phenotype:GO:0015761 mannose transmembrane transport [PMID:2046678]
EXISTENCE:mutant phenotype:GO:1904659 glucose transmembrane transport [PMID:2046678]
Low-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:20854260|PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:1904659 glucose transmembrane transport [PMID:20854260]
Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication
ARS815; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:30679249]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
Protein involved in clathrin-mediated endocytosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0030127 COPII vesicle coat [PMID:11086000|PMID:12426382]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0090110 COPII-coated vesicle cargo loading [PMID:12426382|PMID:12941277]
EXISTENCE:mutant phenotype:GO:0090110 COPII-coated vesicle cargo loading [PMID:12941277]
Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat; COPII coat is required for cargo selection during vesicle formation in ER to Golgi transport; scaffolding function of Lst1p required to generate vesicles that can accommodate difficult cargo proteins that include large oligomeric assemblies and asymmetrically distributed membrane proteins; with Atg40p acts in autophagy of the ER; homologous to Sec24p and Sfb2p
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9885573]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:10911987]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18616809]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:10911987]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20635087]
Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g., Gal4p) which leads to transcription activation; similar to human TRRAP, which is a cofactor for c-Myc mediated oncogenic transformation
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:20485265]
EXISTENCE:direct assay:GO:0008962 phosphatidylglycerophosphatase activity [PMID:20485265]
EXISTENCE:genetic interaction:GO:0008962 phosphatidylglycerophosphatase activity [PMID:20485265]
EXISTENCE:mutant phenotype:GO:0008962 phosphatidylglycerophosphatase activity [PMID:20485265]
EXISTENCE:mutant phenotype:GO:0032049 cardiolipin biosynthetic process [PMID:20485265]
Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase); dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12112232]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:12112232]
Integral membrane protein of the endoplasmic reticulum; required for normal content of cell wall beta-1,6-glucan
putative serine/threonine protein kinase KIC1
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12972564]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:9813095]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12972564]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12972564]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:12480907]
Protein kinase of the PAK/Ste20 family, required for cell integrity; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; phosphorylates Cbk1p kinase after allosteric activation by Hym1p; part of the RAM network that regulates cellular polarity and morphogenesis
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10679005]
Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication
trifunctional aldehyde reductase/xylose reductase/glucose 1-dehydrogenase (NADP(+))
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0004032 alditol:NADP+ 1-oxidoreductase activity [PMID:11306085|PMID:11525399]
EXISTENCE:direct assay:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:direct assay:GO:0032866 D-xylose:NADP reductase activity [PMID:11481678]
EXISTENCE:direct assay:GO:0042843 D-xylose catabolic process [PMID:12271459]
EXISTENCE:direct assay:GO:0071470 cellular response to osmotic stress [PMID:11525399]
EXISTENCE:genetic interaction:GO:0019388 galactose catabolic process [PMID:18811659]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:17919749]
EXISTENCE:mutant phenotype:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:mutant phenotype:GO:0032866 D-xylose:NADP reductase activity [PMID:11481678]
EXISTENCE:mutant phenotype:GO:0042843 D-xylose catabolic process [PMID:12271459]
Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by HOG pathway; overexpression allows xylose fermentation in strains expressing heterologous xylitol dehydrogenase and xylulokinase; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
Endosomal hypothetical protein; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
EXISTENCE:curator inference:GO:0045454 cell redox homeostasis [PMID:10037727]
EXISTENCE:direct assay:GO:0004791 thioredoxin-disulfide reductase activity [PMID:10037727]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10037727|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10037727]
Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:3316985]
EXISTENCE:direct assay:GO:0000921 septin ring assembly [PMID:12636916]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:14993234]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:18550837|PMID:22144691|PMID:29321251]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14597621]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:23345395]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:18552279]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18550837]
EXISTENCE:direct assay:GO:0032160 septin filament array [PMID:15282341]
EXISTENCE:direct assay:GO:0045860 positive regulation of protein kinase activity [PMID:14527412]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:12665577]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:4950437]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:14993234]
EXISTENCE:mutant phenotype:GO:0030011 maintenance of cell polarity [PMID:10882120]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:9442111]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:9864366]
EXISTENCE:physical interaction:GO:0031105 septin complex [PMID:9813094]
Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
phosphatidylinositol 4-phosphate-binding protein
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:10747088]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:15039776]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:18287542]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:10747088]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:15039776]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:10747088]
EXISTENCE:mutant phenotype:GO:0043130 ubiquitin binding [PMID:19574226]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15039776]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:18287542]
Protein that regulates Arf1p and Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; K63-specific Ub chain binding receptor with moderate selectivity; homologous to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000277 [cytochrome c]-lysine N-methyltransferase activity [PMID:10791961]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10791961]
EXISTENCE:direct assay:GO:0018022 peptidyl-lysine methylation [PMID:10791961]
EXISTENCE:mutant phenotype:GO:0000277 [cytochrome c]-lysine N-methyltransferase activity [PMID:10791961]
Cytochrome c lysine methyltransferase; trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth
Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
EXISTENCE:direct assay:GO:0004792 thiosulfate sulfurtransferase activity [PMID:19864628]
EXISTENCE:direct assay:GO:0032447 protein urmylation [PMID:10713047]
EXISTENCE:direct assay:GO:0042292 URM1 activating enzyme activity [PMID:10713047]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:14551258]
EXISTENCE:genetic interaction:GO:0007114 cell budding [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:14551258]
EXISTENCE:mutant phenotype:GO:0002143 tRNA wobble position uridine thiolation [PMID:18664566|PMID:19145231|PMID:19151091]
EXISTENCE:mutant phenotype:GO:0016783 sulfurtransferase activity [PMID:19145231]
EXISTENCE:mutant phenotype:GO:0032447 protein urmylation [PMID:14555475]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:14555475]
EXISTENCE:mutant phenotype:GO:0070733 protein adenylyltransferase activity [PMID:19145231]
EXISTENCE:mutant phenotype:GO:2000220 regulation of pseudohyphal growth [PMID:22043304]
E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21777356]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21777356|PMID:14562095]
hypothetical protein; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:21343297]
EXISTENCE:direct assay:GO:0004177 aminopeptidase activity [PMID:16367759]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0006508 proteolysis [PMID:15606766]
Cytoplasmic aspartyl aminopeptidase with possible vacuole function; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms 12-subunit homo-oligomer; M18 metalloprotease family
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:25620000]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16824951]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:12391157|PMID:16824951]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:16231105]
EXISTENCE:genetic interaction:GO:0009651 response to salt stress [PMID:12391157]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:16824951]
EXISTENCE:mutant phenotype:GO:0045010 actin nucleation [PMID:16824951]
EXISTENCE:physical interaction:GO:0007015 actin filament organization [PMID:12391157]
EXISTENCE:physical interaction:GO:0009651 response to salt stress [PMID:12391157]
SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:18202552]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:23264631]
EXISTENCE:direct assay:GO:0051865 protein autoubiquitination [PMID:18202552]
EXISTENCE:genetic interaction:GO:0000132 establishment of mitotic spindle orientation [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0000921 septin ring assembly [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:21562220]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:15146058|PMID:22570619]
EXISTENCE:genetic interaction:GO:0032186 cellular bud neck septin ring organization [PMID:22570619]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:22570619]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:18202552]
EXISTENCE:mutant phenotype:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:27449057]
EXISTENCE:physical interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:27449057]
Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15145942|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15145942]
EXISTENCE:mutant phenotype:GO:0033108 mitochondrial respiratory chain complex assembly [PMID:19703468]
Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:8663394|PMID:16407407]
EXISTENCE:genetic interaction:GO:0006626 protein targeting to mitochondrion [PMID:18007655]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:18007655]
Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:17053779]
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:16787775|PMID:28483910]
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16959218]
EXISTENCE:genetic interaction:GO:0016279 protein-lysine N-methyltransferase activity [PMID:16143104]
EXISTENCE:genetic interaction:GO:0035066 positive regulation of histone acetylation [PMID:19822662]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27325136]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:11742990|PMID:9398665|PMID:27911222]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:16787775]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16959218]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:16143104]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:17150765]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:9398665]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11752412|PMID:9398665|PMID:9988274|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:32290544]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11752412|PMID:11805083|PMID:12845608]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:27325136]
EXISTENCE:mutant phenotype:GO:1902275 regulation of chromatin organization [PMID:27325136]
EXISTENCE:mutant phenotype:GO:1903341 regulation of meiotic DNA double-strand break formation [PMID:23246437]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Histone methyltransferase, subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3K4; Set1p-dependent H3K4 trimethylation recruits Nrd1p, allowing efficient termination of snoRNAs and cryptic unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway; modulates histone acetylation levels in promoter proximal regions to ensure efficient Nrd1p-dependent termination; required in transcriptional silencing near telomeres and at silent mating type loci; has a SET domain
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:7961999]
EXISTENCE:direct assay:GO:0032137 guanine/thymine mispair binding [PMID:7961998]
EXISTENCE:direct assay:GO:0032139 dinucleotide insertion or deletion binding [PMID:7961998]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:1334021]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:1334021]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:15611870]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:15611870|PMID:1334021]
EXISTENCE:mutant phenotype:GO:0043504 mitochondrial DNA repair [PMID:1334021]
DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:20368989|PMID:26777405]
EXISTENCE:physical interaction:GO:0016070 RNA metabolic process [PMID:16702403]
hypothetical protein that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
iron-sulfur cluster assembly protein CIA2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0097361 CIA complex [PMID:22678362]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:22678362]
Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation and maturation of a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase
EXISTENCE:direct assay:GO:0004142 diacylglycerol cholinephosphotransferase activity [PMID:2848840]
EXISTENCE:direct assay:GO:0004307 ethanolaminephosphotransferase activity [PMID:2848840]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
EXISTENCE:direct assay:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:2848840]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:2848840]
EXISTENCE:mutant phenotype:GO:0004142 diacylglycerol cholinephosphotransferase activity [PMID:2848840]
EXISTENCE:mutant phenotype:GO:0004307 ethanolaminephosphotransferase activity [PMID:2848840]
EXISTENCE:mutant phenotype:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:2848840]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:2848840]
sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11035815]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:9660952]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:9660952]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9660952]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8524222]
Meiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)
Putative GPI protein; SWAT-GFP and mCherry fusion proteins localize to the vacuole; transcription dependent upon Azf1p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:18194531]
hypothetical protein; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:2189783]
EXISTENCE:direct assay:GO:0004845 uracil phosphoribosyltransferase activity [PMID:2189783]
EXISTENCE:direct assay:GO:0008655 pyrimidine-containing compound salvage [PMID:2189783]
Uracil phosphoribosyltransferase; synthesizes UMP from uracil; involved in the pyrimidine salvage pathway
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:9658168]
EXISTENCE:direct assay:GO:0005869 dynactin complex [PMID:9658168]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:11285282]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:8069915]
EXISTENCE:mutant phenotype:GO:0007097 nuclear migration [PMID:8069915]
Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin
hypothetical protein; conserved among S. cerevisiae strains; YHR130C is not an essential gene
hypothetical protein; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762|PMID:14562095]
Conserved fungal protein with similarity to zinc carboxypeptidases; in some strains required for normal cell wall assembly
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:28100785]
EXISTENCE:genetic interaction:GO:1900152 negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:20471941]
EXISTENCE:genetic interaction:GO:1901992 positive regulation of mitotic cell cycle phase transition [PMID:23861665]
EXISTENCE:genetic interaction:GO:1903452 positive regulation of G1 to G0 transition [PMID:23273919|PMID:20471941]
EXISTENCE:genetic interaction:GO:1905184 positive regulation of protein serine/threonine phosphatase activity [PMID:23861665]
Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16270032|PMID:26928762]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0016126 sterol biosynthetic process [PMID:16270032]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:16270032]
EXISTENCE:physical interaction:GO:0016126 sterol biosynthetic process [PMID:16270032]
EXISTENCE:physical interaction:GO:0051082 unfolded protein binding [PMID:16270032]
Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26349035]
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:24998930]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:18336552]
EXISTENCE:direct assay:GO:0008237 metallopeptidase activity [PMID:26349035]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:26349035]
EXISTENCE:direct assay:GO:0019985 translesion synthesis [PMID:24998930]
EXISTENCE:direct assay:GO:0032183 SUMO binding [PMID:26349035]
EXISTENCE:direct assay:GO:0061665 SUMO ligase activity [PMID:26349035]
EXISTENCE:direct assay:GO:1990466 protein autosumoylation [PMID:26349035]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:15598824]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:11606525]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:24998930]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:24998930]
EXISTENCE:mutant phenotype:GO:0010224 response to UV-B [PMID:11606770|PMID:15598824]
EXISTENCE:mutant phenotype:GO:0010225 response to UV-C [PMID:11606770|PMID:15598824]
EXISTENCE:mutant phenotype:GO:0019985 translesion synthesis [PMID:24998930]
EXISTENCE:mutant phenotype:GO:0036205 histone catabolic process [PMID:32130911]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:23357952]
SUMO-ligase and SUMO-targeted metalloprotease; involved in DNA repair; removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; clears chromatin-bound sumoylated proteins; localizes to single spot on nuclear periphery of mother cells but not daughters; exhibits vacuolar localization upon genotoxic stress; activated by DNA binding; member of minigluzincins protease family with mammalian DVC1/Spartan
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8474447]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:1627830|PMID:16622836|PMID:8034689]
EXISTENCE:genetic interaction:GO:0010255 glucose mediated signaling pathway [PMID:27630263]
EXISTENCE:mutant phenotype:GO:0000902 cell morphogenesis [PMID:10198058]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10198058]
EXISTENCE:mutant phenotype:GO:0009749 response to glucose [PMID:14755054]
Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:21143198]
Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
aromatic-amino-acid:2-oxoglutarate transaminase
EXISTENCE:genetic interaction:GO:0006571 tyrosine biosynthetic process [PMID:9491082]
EXISTENCE:genetic interaction:GO:0009094 L-phenylalanine biosynthetic process [PMID:9491082]
EXISTENCE:mutant phenotype:GO:0008793 aromatic-amino-acid:2-oxoglutarate aminotransferase activity [PMID:9491082]
EXISTENCE:mutant phenotype:GO:0009072 aromatic amino acid metabolic process [PMID:9491083]
EXISTENCE:mutant phenotype:GO:0036137 kynurenine aminotransferase activity [PMID:9491082]
Aromatic aminotransferase II; involved in tyrosine, phenylalanine, and methionine metabolism; involved with Aro8p in production of kynurenic acid to detoxify excess tryptophan
EXISTENCE:mutant phenotype:GO:0004866 endopeptidase inhibitor activity [PMID:9657146]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:9657146]
hypothetical protein; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:3280971]
Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin; SPS100 has a paralog, YGP1, that arose from the whole genome duplication
Antisense transcript; upregulates sporulation-specific gene SPS100 upon entry into starvation or sporulation; functions by changing relative expression of SPS100 mRNA isoforms from short, unstable transcript to long, stable species, suggesting novel mechanism of antisense-dependent gene regulation via mRNA isoform switching
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:22842922|PMID:31945465]
Endoplasmic reticulum protein; may contribute to maintenance of ER and nuclear morphology; null mutant shows discontinuity in nuclear envelope, clustering of nuclear pores, and lacks cortical ER
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0000454 snoRNA guided rRNA pseudouridine synthesis [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2191
Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10366589]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:29405545|PMID:22842922]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10366589]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15623581]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:10366589]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:15623581]
hypothetical protein; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:11935221]
EXISTENCE:direct assay:GO:0005621 cellular bud scar [PMID:27229769]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11309124]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:11309124]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:11309124]
Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother; localizes in birth scars; expression is repressed by cAMP
DNA-directed RNA polymerase core subunit RPC10
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:1748681]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:1748681|PMID:3905793]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22842922]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:9837969|PMID:1748681]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:1748681]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:genetic interaction:GO:0006383 transcription by RNA polymerase III [PMID:10531351]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:10531351]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:10531351]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:10531351]
RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress; localizes to the peroxisome in a Pex5p-dependent manner
EXISTENCE:mutant phenotype:GO:0004132 dCMP deaminase activity [PMID:3023902]
EXISTENCE:mutant phenotype:GO:0006226 dUMP biosynthetic process [PMID:6373725]
EXISTENCE:mutant phenotype:GO:0006231 dTMP biosynthetic process [PMID:6373725]
Deoxycytidine monophosphate (dCMP) deaminase; involved in dUMP and dTMP biosynthesis; expression is NOT cell cycle regulated
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:12419258]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12419258]
Protein that binds to cruciform DNA structures; CRP1 has a paralog, MDG1, that arose from the whole genome duplication
ARS818; Autonomously Replicating Sequence
mitochondrial 54S ribosomal protein YmL16
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:9151978|PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the large subunit
snoRNA-binding rRNA-processing protein IMP3
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:23980203]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:15489263|PMID:23980203]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0034457 Mpp10 complex [PMID:17515605]
EXISTENCE:direct assay:GO:0042274 ribosomal small subunit biogenesis [PMID:23980203]
EXISTENCE:mutant phenotype:GO:0032040 small-subunit processome [PMID:12242301]
Component of the SSU processome; SSU processome is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:16314687]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:16314687|PMID:11489916]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11489916]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:16314687]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:16314687]
EXISTENCE:genetic interaction:GO:1902413 negative regulation of mitotic cytokinesis [PMID:16816427]
EXISTENCE:mutant phenotype:GO:1902413 negative regulation of mitotic cytokinesis [PMID:16816427]
Integral membrane protein; localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p; SKG6 has a paralog, TOS2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:12707309]
EXISTENCE:direct assay:GO:0007031 peroxisome organization [PMID:12707309]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:12707309]
Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p
hypothetical protein; mtc6 is synthetically sick with cdc13-1; SWAT-GFP and mCherry fusion proteins localize to the vacuole while SWAT-GFP fusion also localizes to the endoplasmic reticulum
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11729145|PMID:10564265]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11729145]
EXISTENCE:genetic interaction:GO:0031536 positive regulation of exit from mitosis [PMID:11832211]
EXISTENCE:genetic interaction:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
EXISTENCE:mutant phenotype:GO:0007127 meiosis I [PMID:7021311]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:27418100|PMID:11832211]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
Nucleolar hypothetical protein; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000217 DNA secondary structure binding [PMID:30566683]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:18297071]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:18297071]
EXISTENCE:mutant phenotype:GO:0010520 regulation of reciprocal meiotic recombination [PMID:18297071]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:2188099]
EXISTENCE:mutant phenotype:GO:0033235 positive regulation of protein sumoylation [PMID:23326245]
Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:17978089]
EXISTENCE:direct assay:GO:0035361 Cul8-RING ubiquitin ligase complex [PMID:20139071]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:14988729]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:1903775 regulation of DNA double-strand break processing [PMID:26490958]
EXISTENCE:mutant phenotype:GO:1990683 DNA double-strand break attachment to nuclear envelope [PMID:27056668]
EXISTENCE:mutant phenotype:GO:2000001 regulation of DNA damage checkpoint [PMID:26490958]
BRCA1 C-terminal domain protein needed for recovery from DNA damage; implicated in Mms22-dependent DNA repair during S phase; involved in recruiting SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
EXISTENCE:curator inference:GO:0015248 sterol transporter activity [PMID:26001273]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15657396|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0032366 intracellular sterol transport [PMID:26001273]
Putative sterol transfer protein; localizes to puncta in the cortical ER; probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; contains GRAM, StART-like (VASt) and two PH-like domains
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A2946
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12402242]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12402242]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:11720284]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12402242]
Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:15044957]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:9177999]
Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:34965431]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9786949]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9786949]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:23673619]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9786949|PMID:19053807]
EXISTENCE:direct assay:GO:1990615 Kelch-containing formin regulatory complex [PMID:24828508]
EXISTENCE:genetic interaction:GO:0032465 regulation of cytokinesis [PMID:24828508]
EXISTENCE:genetic interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:24828508]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9786949]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:34965431]
EXISTENCE:mutant phenotype:GO:0008360 regulation of cell shape [PMID:9786949]
EXISTENCE:mutant phenotype:GO:0060627 regulation of vesicle-mediated transport [PMID:24828508]
EXISTENCE:mutant phenotype:GO:0090029 negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:34965431]
Protein required for proper cell fusion and cell morphology; acts as phosphorylation-regulated noise suppressor of pheromone signaling pathway; forms a complex with Bud14p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:9864360]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9864360]
EXISTENCE:genetic interaction:GO:0016558 protein import into peroxisome matrix [PMID:9864360]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:9864360]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9864360]
Peroxin; required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p; primarily responsible for peroxisomal import during growth on oleate, and expression is induced during oleate growth
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:33443082]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:9531549]
EXISTENCE:physical interaction:GO:0006897 endocytosis [PMID:9531549]
Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22628558]
EXISTENCE:direct assay:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:25672363]
EXISTENCE:direct assay:GO:0050833 pyruvate transmembrane transporter activity [PMID:25672363]
EXISTENCE:genetic interaction:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:22628554]
EXISTENCE:mutant phenotype:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:22628558]
EXISTENCE:physical interaction:GO:0050833 pyruvate transmembrane transporter activity [PMID:22628558]
Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on fermentable carbon sources, and heterodimerizes with Mpc1p to form the fermentative isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to form the respiratory MPC isoform
EXISTENCE:curator inference:GO:0009051 pentose-phosphate shunt, oxidative branch [PMID:15454531]
EXISTENCE:genetic interaction:GO:0017057 6-phosphogluconolactonase activity [PMID:15454531]
6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:9756935|PMID:23671118]
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:32638513]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:12024033]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:7644470]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19520826|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19520826]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:9710536]
EXISTENCE:direct assay:GO:0017108 5'-flap endonuclease activity [PMID:15448135]
EXISTENCE:direct assay:GO:0032448 DNA hairpin binding [PMID:23344960]
EXISTENCE:direct assay:GO:0043539 protein serine/threonine kinase activator activity [PMID:23355394]
EXISTENCE:direct assay:GO:0044806 G-quadruplex DNA unwinding [PMID:18593712]
EXISTENCE:direct assay:GO:0061849 telomeric G-quadruplex DNA binding [PMID:18593712]
EXISTENCE:genetic interaction:GO:0000706 meiotic DNA double-strand break processing [PMID:20150422]
EXISTENCE:genetic interaction:GO:0000729 DNA double-strand break processing [PMID:21841787]
EXISTENCE:genetic interaction:GO:0033314 mitotic DNA replication checkpoint signaling [PMID:23355394]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:12024033]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:18805091]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:7592912]
EXISTENCE:mutant phenotype:GO:0006273 lagging strand elongation [PMID:10101169|PMID:10330154]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:10101169]
EXISTENCE:mutant phenotype:GO:0032448 DNA hairpin binding [PMID:23344960]
EXISTENCE:mutant phenotype:GO:0043539 protein serine/threonine kinase activator activity [PMID:23355394]
ATP-dependent 5'-flap endonuclease and DNA helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; cell cycle-dependent localization to telomeric chromatin and replicating chromosomes; required for telomerase-dependent telomere synthesis; stability, localization and nuclease activity regulated by sumoylation; forms nuclear foci upon replication stress; human homolog DNA2 complements yeast dna2 mutant
EXISTENCE:direct assay:GO:0000244 spliceosomal tri-snRNP complex assembly [PMID:23393194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17934474|PMID:22842922]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:23393194]
EXISTENCE:direct assay:GO:0030619 U1 snRNA binding [PMID:23393194]
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:23393194]
EXISTENCE:direct assay:GO:0030623 U5 snRNA binding [PMID:23393194|PMID:9848662]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:17934474|PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:0097157 pre-mRNA intronic binding [PMID:23393194]
EXISTENCE:mutant phenotype:GO:0000244 spliceosomal tri-snRNP complex assembly [PMID:17934474]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:22408182|PMID:26968627]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:17626844]
EXISTENCE:mutant phenotype:GO:0000389 mRNA 3'-splice site recognition [PMID:10628969]
EXISTENCE:mutant phenotype:GO:0000395 mRNA 5'-splice site recognition [PMID:29487104]
Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome; mouse ortholog interacts with androgen receptor and may have a role in prostate cancer
anaphase promoting complex subunit CDC23
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:direct assay:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:8895471]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
EXISTENCE:physical interaction:GO:0030332 cyclin binding [PMID:12413490]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
EXISTENCE:direct assay:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:direct assay:GO:0003676 nucleic acid binding [PMID:12093753]
EXISTENCE:mutant phenotype:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0000446 nucleoplasmic THO complex [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:24084588]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12093753]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:27251550]
Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15591131]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:15591131]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:15591131]
Putative GTPase; member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11222764]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:16772403]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11222764]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11222764]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11222764]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:11222764]
ATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esf2p
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10022925]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:11086007|PMID:11313466]
EXISTENCE:direct assay:GO:0043023 ribosomal large subunit binding [PMID:11105761|PMID:17347149]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:24240281]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:23212245]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11086007]
Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:18497569]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10233150|PMID:26928762]
EXISTENCE:direct assay:GO:0006501 C-terminal protein lipidation [PMID:15277523|PMID:19398890]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10233148]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0006501 C-terminal protein lipidation [PMID:11038174|PMID:12965207|PMID:15277523|PMID:11100732]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:23382696]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:10233150|PMID:12965207]
EXISTENCE:mutant phenotype:GO:0019778 Atg12 activating enzyme activity [PMID:10233150]
EXISTENCE:mutant phenotype:GO:0019779 Atg8 activating enzyme activity [PMID:11100732]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10233150|PMID:12965207]
EXISTENCE:mutant phenotype:GO:0032446 protein modification by small protein conjugation [PMID:10233150]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
Autophagy-related protein and dual specificity member of the E1 family; mediates the attachment of Atg12p to Atg5p and Atg8p to phosphatidylethanolamine which are required steps in autophagosome formation; E1 enzymes are also known as ubiquitin-activating enzymes; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
ARS820; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:22842922]
EXISTENCE:direct assay:GO:0005822 inner plaque of spindle pole body [PMID:9215630|PMID:9130700]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:9215630|PMID:9130700]
EXISTENCE:direct assay:GO:0007020 microtubule nucleation [PMID:11950928|PMID:24842996]
EXISTENCE:direct assay:GO:0008275 gamma-tubulin small complex [PMID:9130700|PMID:9384578|PMID:25599398]
EXISTENCE:mutant phenotype:GO:0031122 cytoplasmic microtubule organization [PMID:9670012]
EXISTENCE:physical interaction:GO:0008275 gamma-tubulin small complex [PMID:9130700]
EXISTENCE:physical interaction:GO:0043015 gamma-tubulin binding [PMID:9130700]
Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
hypothetical protein; expressed at both mRNA and protein levels; SWAT-GFP and mCherry fusion proteins localize to the vacuole
EXISTENCE:direct assay:GO:0000015 phosphopyruvate hydratase complex [PMID:6282834]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16565073]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:direct assay:GO:0009898 cytoplasmic side of plasma membrane [PMID:16544286]
EXISTENCE:direct assay:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:16565073]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:mutant phenotype:GO:0004634 phosphopyruvate hydratase activity [PMID:6282834]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:6282834]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:16565073]
Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; mRNA is asymmetrically distributed to daughter cells in a Sir2p-dependent manner, and asymmetry is lost during replicative aging; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11523804]
EXISTENCE:genetic interaction:GO:0005375 copper ion transmembrane transporter activity [PMID:7499355]
EXISTENCE:genetic interaction:GO:0015677 copper ion import [PMID:7499355]
EXISTENCE:mutant phenotype:GO:0005375 copper ion transmembrane transporter activity [PMID:11523804]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:23450501]
EXISTENCE:mutant phenotype:GO:0015680 protein maturation by copper ion transfer [PMID:11523804]
Low-affinity copper transporter of the vacuolar membrane; mutation confers resistance to toxic copper concentrations, while overexpression confers resistance to copper starvation; regulated by nonsense-mediated mRNA decay pathway
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0004499 N,N-dimethylaniline monooxygenase activity [PMID:10600176]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10077572]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:10077572]
Flavin-containing monooxygenase; localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:24994900]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:24994900]
Putative transcription factor containing a WOPR domain; binds DNA in vitro; similar to C. albicans Wor1p transcription factor that regulates white-opaque switching; overexpression causes a cell cycle delay or arrest
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:11943786]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:11943786]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11943786]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:11943786]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16914749]
Transcription factor; involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro
EXISTENCE:direct assay:GO:0003959 NADPH dehydrogenase activity [PMID:8454584]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:17897954]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17897954]
Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:16107716]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16957051]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:16957051]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:16957051]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:30842360|PMID:16957051]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:16107716]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:16107716]
EXISTENCE:mutant phenotype:GO:0090110 COPII-coated vesicle cargo loading [PMID:30842360]
EXISTENCE:mutant phenotype:GO:0097020 COPII receptor activity [PMID:20236934]
EXISTENCE:physical interaction:GO:0097020 COPII receptor activity [PMID:20236934]
ER exit adaptor for Golgi mannosyltransferases; facilitates the exit of Kre2p, Ktr1p and Mnn4p from the ER; involved in COPII-coated vesicle cargo loading and transport; may also promote the retention of proteins in the early Golgi compartment; integral membrane protein of early Golgi and ER
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:32941095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:32941095]
EXISTENCE:direct assay:GO:0030659 cytoplasmic vesicle membrane [PMID:32941095]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:32941095]
EXISTENCE:mutant phenotype:GO:0007163 establishment or maintenance of cell polarity [PMID:32941095]
GTPase activating protein (GAP) for Rho3p, involved in cell polarity green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
phosphogluconate dehydrogenase (decarboxylating) GND1
EXISTENCE:mutant phenotype:GO:0004616 phosphogluconate dehydrogenase (decarboxylating) activity [PMID:1328471]
EXISTENCE:mutant phenotype:GO:0009051 pentose-phosphate shunt, oxidative branch [PMID:1328471]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:9480895]
6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:11742972]
EXISTENCE:direct assay:GO:0070056 prospore membrane leading edge [PMID:11742972|PMID:24390141]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:11742972]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:11742972]
Protein involved in the control of meiotic nuclear division; involved in the coordination of meiosis with spore formation; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; required for assembly of the leading edge coat and both prospore membrane shaping and organization; transcription is induced midway through meiosis
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:11553705]
Sporulation protein required for prospore membrane formation; required for prospore membrane formation at selected spindle poles; ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16394584|PMID:17560372|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16394584]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:12408816]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:12408816]
EXISTENCE:physical interaction:GO:0031931 TORC1 complex [PMID:12408816]
Ubiquitin-binding protein; centrally regulates metabolic homeostasis and carbon utilization during nutrient limitation by managing Snf1p activity; subunit of TORC1 rapamycin-sensitive complex involved in growth control; central scaffold that holds the different components of TORC1 together; contains four HEAT repeats and seven WD-40 repeats
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:22343726]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12139626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12139626]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:22343726]
EXISTENCE:direct assay:GO:0033588 elongator holoenzyme complex [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:15769872]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:22343726]
Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin; localizes to cytosol, nucleus and peroxisomes
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11598210]
EXISTENCE:direct assay:GO:0042765 GPI-anchor transamidase complex [PMID:11598210]
EXISTENCE:mutant phenotype:GO:0016255 attachment of GPI anchor to protein [PMID:11598210]
Subunit of the glycosylphosphatidylinositol transamidase complex; involved in the addition of GPIs to newly synthesized proteins; luminally-oriented, type I integral membrane glycoprotein of the ER; human PIG-T homolog
EXISTENCE:direct assay:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:12799450]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:14576278]
EXISTENCE:genetic interaction:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:12475929]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:12799450]
One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2
bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase
EXISTENCE:direct assay:GO:0004310 farnesyl-diphosphate farnesyltransferase activity [PMID:8157649|PMID:8323279]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762|PMID:1807826]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:8323279]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:350878]
EXISTENCE:direct assay:GO:0051996 squalene synthase activity [PMID:8157649|PMID:8323279]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:1807826|PMID:200835]
Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:11389843|PMID:17483413]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:11389843]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:16525505]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:21347277]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
Subunit of a complex with Ctf18p; shares some subunits with Replication Factor C; required for sister chromatid cohesion
EXISTENCE:direct assay:GO:0071782 endoplasmic reticulum tubular network [PMID:22729086]
EXISTENCE:genetic interaction:GO:0034976 response to endoplasmic reticulum stress [PMID:27117666]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:29915089]
EXISTENCE:mutant phenotype:GO:0071786 endoplasmic reticulum tubular network organization [PMID:22729086]
Lunapark family member involved in ER network formation; regulates the ER asymmetry-induced inheritance block during ER stress; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; required for reticulophagy where it is proposed to stabilize the actin-dependent remodeling of the ER; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0005854 nascent polypeptide-associated complex [PMID:10219998]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:16343487]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:16343487]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:16343487]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:16343487]
EXISTENCE:genetic interaction:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:10518932]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:9482879]
Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15631992]
EXISTENCE:genetic interaction:GO:1900208 regulation of cardiolipin metabolic process [PMID:22403410]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:15631992]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:16020778]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266|PMID:16020778]
Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:28533415]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10888680]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10888680]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:10888680]
EXISTENCE:direct assay:GO:0071562 nucleus-vacuole junction assembly [PMID:10888680]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:16912077]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:12529432]
EXISTENCE:mutant phenotype:GO:0071562 nucleus-vacuole junction assembly [PMID:10888680]
Nuclear envelope protein; anchored to nuclear inner membrane, interacts with vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN); targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:19812255]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309|PMID:12150911|PMID:11914276]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0071528 tRNA re-export from nucleus [PMID:19812255]
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14690591]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:12374754|PMID:15528184]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:19158380]
EXISTENCE:direct assay:GO:0032133 chromosome passenger complex [PMID:19158380]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:19158380]
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates mitotic chromosome segregation; not required for the kinase activity of the complex; mediates the interaction of Sli15p and Bir1p; other complex members are Ipl1p, Sli15p, and Bir1p
proteasome regulatory particle base subunit RPN10
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:8887631]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:20941496]
EXISTENCE:mutant phenotype:GO:0000502 proteasome complex [PMID:15242647]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15242647]
Proteasome polyubiquitin receptor; non-ATPase subunit of the 19S regulatory particle (RP) of the 26S proteasome that links the RP base to the lid; N-terminus plays a role in the assembly of the regulatory particle (RP); ubiquitin-interacting motif selectively binds to polyubiquitin chains; homolog of the mammalian S5a protein
EXISTENCE:direct assay:GO:0004309 exopolyphosphatase activity [PMID:17215253|PMID:7860598|PMID:31175919]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7860598]
EXISTENCE:direct assay:GO:0006798 polyphosphate catabolic process [PMID:8157625]
EXISTENCE:mutant phenotype:GO:0004309 exopolyphosphatase activity [PMID:7860598]
EXISTENCE:mutant phenotype:GO:0006798 polyphosphate catabolic process [PMID:7860598]
Exopolyphosphatase; hydrolyzes inorganic polyphosphate (poly P) into Pi residues; located in the cytosol, plasma membrane, and mitochondrial matrix
ARS822; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:35123996]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11935221]
EXISTENCE:mutant phenotype:GO:0019677 NAD catabolic process [PMID:35123996]
Putative AMP hydrolase; secreted protein proposed to function as an adenosine monophosphatase in NAD+ degradation, via nucleoside salvage pathway that converts NAD+ to AMP and adenosine; overexpression results in adenosine accumulation and reduced levels of NAD+, deletion results in increased NAD+ and NADH; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; similar to human NT5E/CD73 gene involved in cancer progression
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [PMID:21700223|PMID:27053108]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:19279007]
EXISTENCE:direct assay:GO:0036508 protein alpha-1,2-demannosylation [PMID:21700223]
EXISTENCE:direct assay:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:27053108]
EXISTENCE:genetic interaction:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:21700223]
EXISTENCE:mutant phenotype:GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [PMID:19124653]
EXISTENCE:mutant phenotype:GO:0030246 carbohydrate binding [PMID:11375935]
EXISTENCE:mutant phenotype:GO:0097466 ubiquitin-dependent glycoprotein ERAD pathway [PMID:11254655|PMID:11375935]
EXISTENCE:mutant phenotype:GO:1904380 endoplasmic reticulum mannose trimming [PMID:27053108]
EXISTENCE:mutant phenotype:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:19124653|PMID:27053108]
Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; involved in glycan trimming of both folded and misfolded glycoproteins; complexes with Pdi1p, and trims a mannose from Man8GlcNac2 glycans to generate Man7GlcNac2, an oligosaccharide signal on glycoproteins destined for ER-associated protein degradation; requires Pdi1p for stability and substrate recognition; human homolog EDEM1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15466158|PMID:17560372|PMID:19748353|PMID:22842922]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:17568771]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:17568771|PMID:25921291]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15466158]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19159085|PMID:14562095]
EXISTENCE:genetic interaction:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:19684113]
EXISTENCE:genetic interaction:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:19684113]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:17568771]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:19684113]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:19684113]
EXISTENCE:mutant phenotype:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:19684113]
EXISTENCE:mutant phenotype:GO:0047484 regulation of response to osmotic stress [PMID:17568771]
EXISTENCE:mutant phenotype:GO:0060963 positive regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15466158]
EXISTENCE:mutant phenotype:GO:0090153 regulation of sphingolipid biosynthetic process [PMID:24196832]
EXISTENCE:mutant phenotype:GO:1901494 regulation of cysteine metabolic process [PMID:31582588]
EXISTENCE:mutant phenotype:GO:1904828 positive regulation of hydrogen sulfide biosynthetic process [PMID:31582588]
AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
kinase-regulated stress-responsive transcription factor SKN7
EXISTENCE:direct assay:GO:0000156 phosphorelay response regulator activity [PMID:11073911]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:11854400|PMID:9118942]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10888672|PMID:22932476]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11854400|PMID:9118942]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
EXISTENCE:mutant phenotype:GO:0000156 phosphorelay response regulator activity [PMID:11073911]
EXISTENCE:mutant phenotype:GO:0000304 response to singlet oxygen [PMID:16862604]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:9118942]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:17965252]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:9118942]
EXISTENCE:mutant phenotype:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:27373166]
Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
S-adenosylmethionine-dependent methyltransferase
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:22343720]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:22343720]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:27911222]
Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
branched-chain-amino-acid transaminase BAT1
EXISTENCE:direct assay:GO:0004084 branched-chain-amino-acid transaminase activity [PMID:8798704]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8798704]
EXISTENCE:direct assay:GO:0009082 branched-chain amino acid biosynthetic process [PMID:8798704]
EXISTENCE:mutant phenotype:GO:0009082 branched-chain amino acid biosynthetic process [PMID:8798704]
EXISTENCE:mutant phenotype:GO:0009083 branched-chain amino acid catabolic process [PMID:8702755]
Mitochondrial branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA biosynthesis; homolog of murine ECA39; highly expressed during logarithmic phase and repressed during stationary phase; BAT1 has a paralog, BAT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:22028670]
EXISTENCE:mutant phenotype:GO:0008168 methyltransferase activity [PMID:22028670]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:22028670]
S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9483793]
EXISTENCE:mutant phenotype:GO:0000128 flocculation [PMID:19420680|PMID:19087208]
EXISTENCE:mutant phenotype:GO:0005537 mannose binding [PMID:2665372]
EXISTENCE:mutant phenotype:GO:0031589 cell-substrate adhesion [PMID:19087208]
Lectin-like cell wall protein (flocculin) involved in flocculation; binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin resistant but heat labile; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO5 has a paralog, FLO1, that arose from a segmental duplication
Pseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YHR212W-A has a paralog, YAR061W, that arose from a segmental duplication
Pseudogenic fragment with similarity to flocculins; YHR213W has a paralog, YAR062W, that arose from a segmental duplication
Pseudogenic fragment with similarity to flocculins; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; YHR213W-B has a paralog, YAR064W, that arose from a segmental duplication
hypothetical protein; predicted to be a glycosylphosphatidylinositol-modified (GPI) protein; YHR214W has a paralog, YAR066W, that arose from a segmental duplication
YHRCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes; YHR214C-B has a paralog, YAR070C, that arose from a segmental duplication
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; SWAT-GFP and mCherry fusion proteins localize to the nucleus and endoplasmic reticulum respectively; YHR214C-D has a paralog, YAR069C, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0003993 acid phosphatase activity [PMID:8817921]
One of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; pregulated by phosphate starvation; PHO12 has a paralog, PHO11, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:20097157]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:20097157]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in GTP biosynthesis, expression is induced by mycophenolic acid resulting in resistance to the drug, expression is repressed by nutrient limitation; IMD2 has a paralog, YAR073W/YAR075W, that arose from a segmental duplication
ARS824; Autonomously Replicating Sequence
TEL08R; Telomeric region on the right arm of Chromosome VIII; composed of an X element core sequence, X element combinatorial repeats, an internal stretch of telomeric repeats, a short Y' element, and a terminal stretch of telomeric repeats
Helicase-like protein encoded within the telomeric Y' element
hypothetical protein with similarity to helicases; located in the telomere region on the right arm of chromosome VIII