Saccharomyces cerevisiae S288C chromosome VII, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome VII
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006941.\n\nOn Apr 26, 2011 this sequence version replaced NC_001139.8.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL07L; Telomeric region on the left arm of Chromosome VII; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
hypothetical protein; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
hypothetical protein; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
Protein with similarity to GPI-anchored aspartic proteases; such proteases are Yap1p and Yap3p; mCherry fusion protein localizes to the vacuole
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11935221]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
hypothetical protein; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:30700649]
EXISTENCE:genetic interaction:GO:0000033 alpha-1,3-mannosyltransferase activity [PMID:10521541]
EXISTENCE:genetic interaction:GO:0006493 protein O-linked glycosylation [PMID:10521541]
EXISTENCE:mutant phenotype:GO:0000033 alpha-1,3-mannosyltransferase activity [PMID:10521541]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:10521541]
Mannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:3282541]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095|PMID:24769239]
EXISTENCE:genetic interaction:GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:3023838]
EXISTENCE:mutant phenotype:GO:0006113 fermentation [PMID:3023838]
Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
EXISTENCE:mutant phenotype:GO:0000006 high-affinity zinc transmembrane transporter activity [PMID:8637895]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:8637895]
EXISTENCE:mutant phenotype:GO:0071578 zinc ion import across plasma membrane [PMID:8637895]
High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10234785]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10234785|PMID:35809138]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10234785]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10234785]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10234785]
EXISTENCE:mutant phenotype:GO:0018890 cyanamide metabolic process [PMID:35809138]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:35809138|PMID:10870099]
Transcription factor involved in sulfite metabolism; activates transcription of SSU1, DDI2, DDI3; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004396 hexokinase activity [PMID:12611889|PMID:332086]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11311123|PMID:9563516]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11311123|PMID:9563516]
EXISTENCE:direct assay:GO:0006013 mannose metabolic process [PMID:332086]
EXISTENCE:direct assay:GO:0006096 glycolytic process [PMID:332086|PMID:8917466]
EXISTENCE:direct assay:GO:0046015 regulation of transcription by glucose [PMID:11311123]
EXISTENCE:genetic interaction:GO:0046323 glucose import [PMID:6300872]
EXISTENCE:genetic interaction:GO:1990539 fructose import across plasma membrane [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0004396 hexokinase activity [PMID:6341351]
EXISTENCE:mutant phenotype:GO:0006000 fructose metabolic process [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0006006 glucose metabolic process [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:8917466]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0031966 mitochondrial membrane [PMID:31862471]
EXISTENCE:mutant phenotype:GO:2001234 negative regulation of apoptotic signaling pathway [PMID:31862471]
Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:12446794]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10848632]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7493987]
EXISTENCE:direct assay:GO:0051219 phosphoprotein binding [PMID:14536080]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7493987]
EXISTENCE:mutant phenotype:GO:0001079 nitrogen catabolite regulation of transcription from RNA polymerase II promoter [PMID:11687605]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:8422683]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:11076970]
EXISTENCE:mutant phenotype:GO:0031930 mitochondria-nucleus signaling pathway [PMID:7493987|PMID:8422683]
EXISTENCE:mutant phenotype:GO:0034504 protein localization to nucleus [PMID:10848632]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:12399373]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7493987]
Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:28051769]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:11971962]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11971962]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:12039965]
EXISTENCE:direct assay:GO:0051321 meiotic cell cycle [PMID:11971962]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:15805472]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15066280]
EXISTENCE:mutant phenotype:GO:1990166 protein localization to site of double-strand break [PMID:28051769]
Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006311 meiotic gene conversion [PMID:18069899]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:18069899]
Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
EXISTENCE:direct assay:GO:0000217 DNA secondary structure binding [PMID:30566683]
EXISTENCE:direct assay:GO:0000795 synaptonemal complex [PMID:9590170]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:9590170]
EXISTENCE:mutant phenotype:GO:0033235 positive regulation of protein sumoylation [PMID:23326245]
Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
Antisense transcript that represses the meiosis-specific ZIP2 gene; expressed in haploid cells under sporulation inducing conditions; regulates ZIP2 in a cis-dependent manner; RME3 is repressed by the MAT a1/alpha2 complex in diploid cells
3',5'-cyclic-nucleotide phosphodiesterase PDE1
EXISTENCE:genetic interaction:GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity [PMID:2824992]
EXISTENCE:mutant phenotype:GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity [PMID:2824992|PMID:6300049]
EXISTENCE:mutant phenotype:GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway [PMID:9880329]
Low-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:30133335]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11483521|PMID:26432634]
EXISTENCE:genetic interaction:GO:0055088 lipid homeostasis [PMID:20016074|PMID:26432634]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:30133335]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:20016074]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:26432634|PMID:20016074]
Essential nuclear envelope integral membrane protein; interacts with chromatin, helping to maintain normal chromatin architecture and transcriptional regulation of certain loci at the nuclear envelope; interacts and functions with Apq12p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA nuclear export, and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; homologous to Brl1p
EXISTENCE:direct assay:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:26638174]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12612077|PMID:22932476]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:10805743]
EXISTENCE:direct assay:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:20802481]
EXISTENCE:direct assay:GO:0034353 mRNA 5'-diphosphatase activity [PMID:20802481]
EXISTENCE:direct assay:GO:1990174 phosphodiesterase decapping endonuclease activity [PMID:20802481]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10805743]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10805743]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:20802481]
EXISTENCE:mutant phenotype:GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled [PMID:15565157]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:16131592]
EXISTENCE:mutant phenotype:GO:0110155 NAD-cap decapping [PMID:33139726]
EXISTENCE:mutant phenotype:GO:1990174 phosphodiesterase decapping endonuclease activity [PMID:20802481]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:10805743]
Nuclear decapping endonuclease; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; involved in an early step of decapping non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs), part of ncRNA surveillance; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0004818 glutamate-tRNA ligase activity [PMID:11069915]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19417106]
EXISTENCE:direct assay:GO:0006424 glutamyl-tRNA aminoacylation [PMID:11069915]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405|PMID:26359986]
EXISTENCE:direct assay:GO:0017102 methionyl glutamyl tRNA synthetase complex [PMID:11069915]
Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:12682017]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:20732871]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15643076]
EXISTENCE:direct assay:GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding [PMID:22796944]
EXISTENCE:direct assay:GO:2001173 regulation of histone H2B conserved C-terminal lysine ubiquitination [PMID:19531475]
EXISTENCE:direct assay:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11884586]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15180994]
EXISTENCE:mutant phenotype:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23109428]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15149594]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:14710186]
EXISTENCE:mutant phenotype:GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [PMID:18469135]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:32290544]
EXISTENCE:mutant phenotype:GO:0051569 regulation of histone H3-K4 methylation [PMID:12667454|PMID:12876293|PMID:12876294|PMID:15180994]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:2001160 regulation of histone H3-K79 methylation [PMID:12876293]
EXISTENCE:mutant phenotype:GO:2001163 regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:15149594]
EXISTENCE:mutant phenotype:GO:2001165 positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:18469135]
EXISTENCE:mutant phenotype:GO:2001166 regulation of histone H2B ubiquitination [PMID:12876293|PMID:12876294]
EXISTENCE:mutant phenotype:GO:2001173 regulation of histone H2B conserved C-terminal lysine ubiquitination [PMID:17576814]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:11884586|PMID:11927560]
Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:9707437]
EXISTENCE:direct assay:GO:0043829 tRNA-specific adenosine-37 deaminase activity [PMID:9707437]
tRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala
hypothetical protein; N-terminally propionylated in vivo; deletion mutant is viable
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10385521|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10385521|PMID:22842922]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:15888545]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:15888545|PMID:10385521]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:12456659]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:10385521]
EXISTENCE:physical interaction:GO:0061608 nuclear import signal receptor activity [PMID:10385521]
Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:12402045]
EXISTENCE:direct assay:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:12402045]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:12402045]
EXISTENCE:direct assay:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:12402045]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:12399376]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:9348530|PMID:12402045]
EXISTENCE:mutant phenotype:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:12402045]
Processivity factor; required for ubiquitination activity of anaphase promoting complex (APC), mediates the activity of APC by contributing to substrate recognition; involved in cyclin proteolysis; contains conserved DOC1 homology domain
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:9857050]
EXISTENCE:genetic interaction:GO:0005049 nuclear export signal receptor activity [PMID:9857050]
EXISTENCE:genetic interaction:GO:0006611 protein export from nucleus [PMID:9857050]
EXISTENCE:mutant phenotype:GO:0061015 snRNA import into nucleus [PMID:31189105]
EXISTENCE:physical interaction:GO:0005049 nuclear export signal receptor activity [PMID:9857050]
EXISTENCE:physical interaction:GO:0006611 protein export from nucleus [PMID:9857050]
Nuclear envelope protein that acts as a recycling factor; mediates the nuclear export of Srp1p (importin alpha) back to the cytoplasm after its import substrates have been released into the nucleoplasm, thereby allowing the participation of Srp1p in multiple rounds of nuclear import; required for accurate chromosome segregation; homolog of metazoan CAS and human CSE1L, overexpression of which is implicated in cancer progression
EXISTENCE:direct assay:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:2832951]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7828851]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:2832951]
EXISTENCE:direct assay:GO:0016602 CCAAT-binding factor complex [PMID:2832951]
EXISTENCE:mutant phenotype:GO:0043457 regulation of cellular respiration [PMID:2182199]
EXISTENCE:physical interaction:GO:0016602 CCAAT-binding factor complex [PMID:2832951]
Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9774408|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0070899 mitochondrial tRNA wobble uridine modification [PMID:15509579]
Mitochondrial protein; forms heterodimer complex with Mss1p that performs 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants; human homolog MTO1 can complement yeast null mutant
hypothetical protein; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
bifunctional aminoimidazole ribotide synthase/glycinamide ribotide synthase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
Bifunctional enzyme of 'de novo' purine nucleotide pathway; contains both phosphoribosylglycinamidine synthetase/glycinamide ribotide synthetase (EC 6.3.4.13) ['Ade5'] and phosphoribosylaminoimidazole synthetase/aminoimidazole ribotide synthetase (EC 6.3.3.1) ['Ade7'] activities
Rab GTPase-binding exocyst subunit SEC15
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:16478783]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:1512289|PMID:7615633|PMID:8978675]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:16478783]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:16478783|PMID:26928762]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:10022848]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15037780]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:15037780]
Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4 and fly CG11137; mutation is functionally complemented by human EMC4
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
hypothetical protein; non-essential gene
ARS702; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8649382]
Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly
glucose-induced degradation complex subunit VID30
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:16872538|PMID:18508925]
EXISTENCE:mutant phenotype:GO:0006808 regulation of nitrogen utilization [PMID:11356843]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616|PMID:15358789]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
dolichyl-diphosphooligosaccharide--protein glycotransferase subunit
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12805367]
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084|PMID:12805367]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9135133]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:9405463]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005458 GDP-mannose transmembrane transporter activity [PMID:20576760]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:9395539]
EXISTENCE:mutant phenotype:GO:0005458 GDP-mannose transmembrane transporter activity [PMID:9395539]
EXISTENCE:mutant phenotype:GO:1990570 GDP-mannose transmembrane transport [PMID:9395539]
Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008252 nucleotidase activity [PMID:11934891]
EXISTENCE:mutant phenotype:GO:0006206 pyrimidine nucleobase metabolic process [PMID:11934891]
Pyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000301 retrograde transport, vesicle recycling within Golgi [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0007030 Golgi organization [PMID:15932880]
EXISTENCE:mutant phenotype:GO:0017119 Golgi transport complex [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:15932880]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20065092]
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:12554867]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:12554867]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19682251]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19682251|PMID:14562095]
EXISTENCE:direct assay:GO:0098745 Dcp1-Dcp2 complex [PMID:11139489]
EXISTENCE:mutant phenotype:GO:0032056 positive regulation of translation in response to stress [PMID:19682251]
EXISTENCE:physical interaction:GO:0098745 Dcp1-Dcp2 complex [PMID:11139489]
RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17034789|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17034789|PMID:14562095|PMID:16823961|PMID:24769239]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18281282]
EXISTENCE:direct assay:GO:0051536 iron-sulfur cluster binding [PMID:31493153]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18281282]
Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:14981098|PMID:16407407]
EXISTENCE:genetic interaction:GO:0015914 phospholipid transport [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266]
EXISTENCE:mutant phenotype:GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering [PMID:19556461]
EXISTENCE:physical interaction:GO:0032865 ERMES complex [PMID:21945531]
Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
EXISTENCE:direct assay:GO:0005880 nuclear microtubule [PMID:9693366]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:9693366]
EXISTENCE:direct assay:GO:0008574 plus-end-directed microtubule motor activity [PMID:16906145|PMID:16906148]
EXISTENCE:direct assay:GO:0070462 plus-end specific microtubule depolymerization [PMID:16906145|PMID:16906148]
EXISTENCE:direct assay:GO:0070463 tubulin-dependent ATPase activity [PMID:16906148]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:9281582|PMID:16906148]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:33752231]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:10525539]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:9693366]
EXISTENCE:mutant phenotype:GO:0031115 negative regulation of microtubule polymerization [PMID:24616221]
EXISTENCE:mutant phenotype:GO:0032888 regulation of mitotic spindle elongation [PMID:24616221]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:31964755]
EXISTENCE:mutant phenotype:GO:0051228 mitotic spindle disassembly [PMID:23851487|PMID:9813090]
EXISTENCE:mutant phenotype:GO:0090307 mitotic spindle assembly [PMID:23851487]
Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:9032248]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:20417603]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:9032248]
Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
SKI complex subunit WD repeat protein SKI8
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:14992724]
EXISTENCE:direct assay:GO:0055087 Ski complex [PMID:18042677|PMID:10744028]
EXISTENCE:genetic interaction:GO:0007131 reciprocal meiotic recombination [PMID:14992724]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:14992724]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:9482746]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:14992724]
EXISTENCE:mutant phenotype:GO:0065004 protein-DNA complex assembly [PMID:14992724]
EXISTENCE:mutant phenotype:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12881429]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:11910110|PMID:17660569]
EXISTENCE:physical interaction:GO:0007131 reciprocal meiotic recombination [PMID:14992724]
EXISTENCE:physical interaction:GO:0055087 Ski complex [PMID:18042677]
Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11433291|PMID:16984909|PMID:26928762|PMID:27881666]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:26774783]
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:19805279]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:17400548]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:16984909]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0031201 SNARE complex [PMID:9628864]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:27365394]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:17400548]
EXISTENCE:mutant phenotype:GO:0061909 autophagosome-lysosome fusion [PMID:26774783]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:9628864]
Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:19145231]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18664566]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:17592039|PMID:18755837]
EXISTENCE:mutant phenotype:GO:0002143 tRNA wobble position uridine thiolation [PMID:18664566|PMID:19145231|PMID:19151091]
EXISTENCE:mutant phenotype:GO:0032447 protein urmylation [PMID:14551258]
Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae; localizes to cytosol and peroxisomes
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15537705]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15537705]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:15537705]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:9151665]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:9151665]
Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:8756637]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:8756637|PMID:9832517]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22353369|PMID:9832517|PMID:22842922]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22353369]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9858577]
EXISTENCE:genetic interaction:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:8756637|PMID:9832517]
EXISTENCE:genetic interaction:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9832517]
EXISTENCE:genetic interaction:GO:1900436 positive regulation of filamentous growth of a population of unicellular organisms in response to starvation [PMID:22904036]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9858577]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:9832517]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9832517]
Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11331606]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12562756]
EXISTENCE:direct assay:GO:0031588 nucleotide-activated protein kinase complex [PMID:12393914]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:12556493]
EXISTENCE:genetic interaction:GO:0004679 AMP-activated protein kinase activity [PMID:10990457]
EXISTENCE:genetic interaction:GO:0007165 signal transduction [PMID:10990457]
EXISTENCE:genetic interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:9121458]
EXISTENCE:genetic interaction:GO:0140767 enzyme-substrate adaptor activity [PMID:27592031]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:10921902]
EXISTENCE:physical interaction:GO:0031588 nucleotide-activated protein kinase complex [PMID:7813428|PMID:9121458]
One of three alternate beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10413469]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10413469]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:19683499]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:12952948|PMID:15082784]
EXISTENCE:direct assay:GO:0007063 regulation of sister chromatid cohesion [PMID:31582854]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:11432837]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:18089575]
EXISTENCE:direct assay:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15987999]
EXISTENCE:genetic interaction:GO:0035101 FACT complex [PMID:11432837]
EXISTENCE:genetic interaction:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:15987999]
EXISTENCE:mutant phenotype:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:19574230]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27226635]
EXISTENCE:physical interaction:GO:0006261 DNA-templated DNA replication [PMID:9199353]
EXISTENCE:physical interaction:GO:0035101 FACT complex [PMID:11432837]
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization
EXISTENCE:direct assay:GO:0006897 endocytosis [PMID:16643280]
EXISTENCE:genetic interaction:GO:0030866 cortical actin cytoskeleton organization [PMID:12181333]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:27062026]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16643280|PMID:27062026]
EXISTENCE:mutant phenotype:GO:0030866 cortical actin cytoskeleton organization [PMID:12181333]
Clathrin heavy chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion; the light chain (CLC1) is thought to regulate function
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:11967269]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:12819196]
Fatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix; component of a light sensing pathway, converting light into a hydrogen peroxide signal, modulating nucleo-cytoplasmic shuttling of stress-responsive transcription factor, Msn2p
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis
hypothetical protein; mRNA identified as translated by ribosome profiling data; protein identified via enrichment-based proteogenomics; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
ARS704; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004185 serine-type carboxypeptidase activity [PMID:2668738]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:8308064]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:23656787]
Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase
EXISTENCE:direct assay:GO:0047536 2-aminoadipate transaminase activity [PMID:19342587]
EXISTENCE:genetic interaction:GO:0006571 tyrosine biosynthetic process [PMID:9491082]
EXISTENCE:genetic interaction:GO:0009094 L-phenylalanine biosynthetic process [PMID:9491082]
EXISTENCE:mutant phenotype:GO:0008793 aromatic-amino-acid:2-oxoglutarate aminotransferase activity [PMID:9491082]
EXISTENCE:mutant phenotype:GO:0009072 aromatic amino acid metabolic process [PMID:9491083]
EXISTENCE:mutant phenotype:GO:0047536 2-aminoadipate transaminase activity [PMID:9491082]
Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis; involved with Aro9p in production of kynurenic acid to detoxify excess tryptophan
ARS706; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:15723534]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10704410]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10704410]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:15723534|PMID:13679365]
EXISTENCE:direct assay:GO:0009378 four-way junction helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:15723534|PMID:18657510]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:direct assay:GO:0042555 MCM complex [PMID:12480933]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:direct assay:GO:0097373 MCM core complex [PMID:13679365]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10834843]
Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8862519]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:8862519]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8862519]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:8862519]
Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:17178117]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:11943201]
Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
EXISTENCE:genetic interaction:GO:0075297 negative regulation of ascospore formation [PMID:9383076]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
EXISTENCE:mutant phenotype:GO:0031929 TOR signaling [PMID:20457806]
EXISTENCE:mutant phenotype:GO:0075297 negative regulation of ascospore formation [PMID:9383076]
Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0008721 D-serine ammonia-lyase activity [PMID:17937657|PMID:17869212]
EXISTENCE:mutant phenotype:GO:0070178 D-serine metabolic process [PMID:17937657]
D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9234705]
EXISTENCE:direct assay:GO:0016239 positive regulation of macroautophagy [PMID:28057755]
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:9234705]
EXISTENCE:mutant phenotype:GO:0006448 regulation of translational elongation [PMID:9234705]
EXISTENCE:mutant phenotype:GO:0019887 protein kinase regulator activity [PMID:11101534]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:28057755]
Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA; suppresses frameshifting at stalled ribosomes
hypothetical protein; identified based on comparisons of the genome sequences of six Saccharomyces species; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:direct assay:GO:0045129 NAD-independent histone deacetylase activity [PMID:11711434]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:19379692]
EXISTENCE:mutant phenotype:GO:0032874 positive regulation of stress-activated MAPK cascade [PMID:18487345]
EXISTENCE:mutant phenotype:GO:0045835 negative regulation of meiotic nuclear division [PMID:11711434]
Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
Antisense transcript initiated from the 3' end of the IME4 locus; expressed in haploids and interferes with IME4 expression through a cis-acting mechanism; RME2 expression is repressed by the MAT a1/alpha2 repressor complex
Haploid-specific gene repressed by a1-alpha2; turned off in sir3 null strains, absence enhances the sensitivity of rad52-327 cells to campothecin almost 100-fold
EXISTENCE:direct assay:GO:0036396 RNA N6-methyladenosine methyltransferase complex [PMID:22685417]
EXISTENCE:genetic interaction:GO:0080009 mRNA methylation [PMID:22685417]
EXISTENCE:mutant phenotype:GO:0001734 mRNA (N6-adenosine)-methyltransferase activity [PMID:12384598]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:1545790|PMID:12384598|PMID:22685417]
EXISTENCE:mutant phenotype:GO:0080009 mRNA methylation [PMID:12384598|PMID:22685417]
EXISTENCE:mutant phenotype:GO:1902974 meiotic DNA replication initiation [PMID:31363087]
EXISTENCE:mutant phenotype:GO:2000221 negative regulation of pseudohyphal growth [PMID:22685417]
mRNA N6-adenosine methyltransferase; essential for meiosis in S288C but dispensable for meiosis in SK1, mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70)
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24478450|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:10429195]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8395517]
EXISTENCE:mutant phenotype:GO:0097250 mitochondrial respirasome assembly [PMID:22342701]
Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
protein phosphatase 2A regulatory subunit CDC55
EXISTENCE:direct assay:GO:0000159 protein phosphatase type 2A complex [PMID:8600023]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12388751]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12388751]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21536748]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12388751]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12388751]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12388751]
EXISTENCE:genetic interaction:GO:0019888 protein phosphatase regulator activity [PMID:17550305]
EXISTENCE:genetic interaction:GO:0044818 mitotic G2/M transition checkpoint [PMID:20980617]
EXISTENCE:genetic interaction:GO:0045143 homologous chromosome segregation [PMID:27455870]
EXISTENCE:genetic interaction:GO:2000786 positive regulation of autophagosome assembly [PMID:27973551]
EXISTENCE:mutant phenotype:GO:0000159 protein phosphatase type 2A complex [PMID:8600023]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:16314395]
EXISTENCE:mutant phenotype:GO:0010971 positive regulation of G2/M transition of mitotic cell cycle [PMID:21536748]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:32029270]
EXISTENCE:mutant phenotype:GO:0061586 positive regulation of transcription by transcription factor localization [PMID:23275436]
EXISTENCE:mutant phenotype:GO:0090266 regulation of mitotic cell cycle spindle assembly checkpoint [PMID:23886942|PMID:27191586]
EXISTENCE:mutant phenotype:GO:1900182 positive regulation of protein localization to nucleus [PMID:23275436]
EXISTENCE:mutant phenotype:GO:1905477 positive regulation of protein localization to membrane [PMID:32029270]
Regulatory subunit B of protein phosphatase 2A (PP2A); Zds1p/2p-dependent localization to cytoplasm promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division, chromosome segregation during achiasmate meiosis; maintains nucleolar sequestration of Cdc14p in early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; controls phosphorylation and stability of Cln2p; homolog of mammalian B55
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25144938]
EXISTENCE:mutant phenotype:GO:0042255 ribosome assembly [PMID:27303706]
EXISTENCE:physical interaction:GO:0030686 90S preribosome [PMID:25144938]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26A has a paralog, RPS26B, that arose from the whole genome duplication; human homolog can partially complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:17215247]
Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12649274]
EXISTENCE:direct assay:GO:0051180 vitamin transport [PMID:12649274]
EXISTENCE:direct assay:GO:0090482 vitamin transmembrane transporter activity [PMID:12649274]
Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
EXISTENCE:mutant phenotype:GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor [PMID:17173333]
Putative protein with sequence similar to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0004121 cystathionine beta-lyase activity [PMID:21478306]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:11565790]
EXISTENCE:direct assay:GO:0047804 cysteine-S-conjugate beta-lyase activity [PMID:29436228]
EXISTENCE:mutant phenotype:GO:0004121 cystathionine beta-lyase activity [PMID:10821189]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:10821189]
EXISTENCE:mutant phenotype:GO:0019346 transsulfuration [PMID:10821189]
Peroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:11940665]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:15249670]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:11470404|PMID:16581767]
Protein required for recombination and meiotic nuclear division; forms a complex with Hop2p, which is involved in chromosome pairing and repair of meiotic double-strand breaks
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11549884|PMID:17449140]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:17449140]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:18208507]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:17449140]
EXISTENCE:mutant phenotype:GO:0010512 negative regulation of phosphatidylinositol biosynthetic process [PMID:18208507]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12782635]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:16911513]
EXISTENCE:physical interaction:GO:0140297 DNA-binding transcription factor binding [PMID:16911513]
Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
EXISTENCE:direct assay:GO:0000045 autophagosome assembly [PMID:18725539]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:11689437|PMID:15659643|PMID:18497569]
EXISTENCE:direct assay:GO:0000421 autophagosome membrane [PMID:22885598]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:22885598|PMID:25921291|PMID:9224897|PMID:33536246]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9190802|PMID:26928762]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:direct assay:GO:0120095 vacuole-isolation membrane contact site [PMID:23549786]
EXISTENCE:direct assay:GO:1990316 Atg1/ULK1 kinase complex [PMID:26753620]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:22778255|PMID:9224897]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:9224897]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:23382696]
EXISTENCE:mutant phenotype:GO:0006995 cellular response to nitrogen starvation [PMID:33536246]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:32788210]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:33536246]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10995454|PMID:12589048]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:33536246]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
EXISTENCE:physical interaction:GO:1990316 Atg1/ULK1 kinase complex [PMID:15743910]
Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; forms a complex with Atg13p and Atg17p during autophagy; required for re-phosphorylation of Atg13p during termination of autophagy, following prolonged nitrogen starvation; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
ARS707; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:12847291|PMID:16201971]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15879520|PMID:11914276]
EXISTENCE:mutant phenotype:GO:1900180 regulation of protein localization to nucleus [PMID:15340085]
Protein kinase; related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; localizes to cytosol and peroxisomes; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11157761|PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20498834|PMID:22842922|PMID:14562095]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:20427513]
EXISTENCE:direct assay:GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:16715093]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:29297464]
EXISTENCE:mutant phenotype:GO:1902416 positive regulation of mRNA binding [PMID:22890846]
EXISTENCE:mutant phenotype:GO:1903450 regulation of G1 to G0 transition [PMID:31141453]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:16715093|PMID:20705650]
EXISTENCE:physical interaction:GO:0045947 negative regulation of translational initiation [PMID:20705650]
mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
hypothetical protein; contributes to high inhibitor stress tolerance; deletion mutant is viable
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:18042458]
EXISTENCE:direct assay:GO:0000737 DNA catabolic process, endonucleolytic [PMID:18042458]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:18042458]
EXISTENCE:direct assay:GO:0030870 Mre11 complex [PMID:25231868]
EXISTENCE:direct assay:GO:1905779 positive regulation of exonuclease activity [PMID:25231868]
EXISTENCE:genetic interaction:GO:0000706 meiotic DNA double-strand break processing [PMID:20150422]
EXISTENCE:genetic interaction:GO:0000729 DNA double-strand break processing [PMID:18806779]
EXISTENCE:genetic interaction:GO:0042138 meiotic DNA double-strand break formation [PMID:22745819]
EXISTENCE:mutant phenotype:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:18042458]
EXISTENCE:mutant phenotype:GO:0000706 meiotic DNA double-strand break processing [PMID:9215888]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:19595717]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:26490958]
EXISTENCE:mutant phenotype:GO:0000737 DNA catabolic process, endonucleolytic [PMID:18042458]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:18042458]
EXISTENCE:mutant phenotype:GO:0007534 gene conversion at mating-type locus [PMID:18806779]
EXISTENCE:mutant phenotype:GO:0010791 DNA double-strand break processing involved in repair via synthesis-dependent strand annealing [PMID:23254329]
EXISTENCE:mutant phenotype:GO:0031860 telomeric 3' overhang formation [PMID:19595717]
EXISTENCE:mutant phenotype:GO:0035306 positive regulation of dephosphorylation [PMID:25831494]
Endonuclease required for telomere elongation; required for telomeric 5' C-rich strand resection; involved in ds-break repair and processing hairpin DNA structures with the MRX complex; function requires sumoylation and phosphorylation; exists as inactive oligomers that are transiently released into smaller active units by phosphorylation; DNA damage triggers Sae2p removal, so active Sae2p is present only transiently; sequence and functional similarity with human CtIP/RBBP8
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:15565172]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11452010]
EXISTENCE:direct assay:GO:0070274 RES complex [PMID:15565172]
Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
EXISTENCE:direct assay:GO:0000932 P-body [PMID:12730603|PMID:25811606]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23706738]
EXISTENCE:direct assay:GO:0004534 5'-3' exoribonuclease activity [PMID:3545079]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23706738|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640]
EXISTENCE:direct assay:GO:0016078 tRNA catabolic process [PMID:21632824]
EXISTENCE:direct assay:GO:0031370 eukaryotic initiation factor 4G binding [PMID:30899024]
EXISTENCE:direct assay:GO:0090512 eisosome membrane domain/MCC [PMID:25811606]
EXISTENCE:direct assay:GO:0110155 NAD-cap decapping [PMID:35173156]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:9488433]
EXISTENCE:genetic interaction:GO:0043144 sno(s)RNA processing [PMID:9488433]
EXISTENCE:mutant phenotype:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:10848586]
EXISTENCE:mutant phenotype:GO:0000932 P-body [PMID:12730603]
EXISTENCE:mutant phenotype:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:24119399]
EXISTENCE:mutant phenotype:GO:0004534 5'-3' exoribonuclease activity [PMID:1979303]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9488433]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:30899024]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:16240118]
EXISTENCE:mutant phenotype:GO:0016242 negative regulation of macroautophagy [PMID:29465287]
EXISTENCE:mutant phenotype:GO:0032204 regulation of telomere maintenance [PMID:28160602]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:23706738]
EXISTENCE:mutant phenotype:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:23706738|PMID:33258404]
EXISTENCE:mutant phenotype:GO:0061157 mRNA destabilization [PMID:24567736]
EXISTENCE:mutant phenotype:GO:0070479 nuclear-transcribed mRNA catabolic process, 5'-3' exonucleolytic nonsense-mediated decay [PMID:30874502]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:19481524]
EXISTENCE:mutant phenotype:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:31913314]
EXISTENCE:mutant phenotype:GO:1901522 positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [PMID:33258404]
Evolutionarily-conserved 5'-3' exonuclease; deNADding enzyme that modulates mitochondrial NAD-capped RNA; component of cytoplasmic P- bodies involved in mRNA decay; enters nucleus, positively regulates transcription initiation and elongation; involved in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, telomere maintenance, and turnover of tRNA introns; negative regulator of autophagy; activated by scavenger decapping enzyme Dcs1p
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11914276]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:1385442]
EXISTENCE:direct assay:GO:0017056 structural constituent of nuclear pore [PMID:19680227]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406|PMID:24574455|PMID:9813081]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906|PMID:9971735]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:8524308]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:7813444]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:7813444]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
One of two upstream open reading frames (uORFs) in 5' untranslated region of ROK1 gene, regulate translation
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:23153376]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9154839]
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:18975973|PMID:23153376]
EXISTENCE:direct assay:GO:0030490 maturation of SSU-rRNA [PMID:23630256]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9154839]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9154839]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9154839]
EXISTENCE:mutant phenotype:GO:0048254 snoRNA localization [PMID:18833290]
RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:24390141]
EXISTENCE:direct assay:GO:0035974 meiotic spindle pole body [PMID:12796288]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12796288]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0035974 meiotic spindle pole body [PMID:12796288]
Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
EXISTENCE:direct assay:GO:0002949 tRNA threonylcarbamoyladenosine modification [PMID:23620299]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25038083]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:19369944]
EXISTENCE:direct assay:GO:0061710 L-threonylcarbamoyladenylate synthase [PMID:23620299]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:19369944]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:19884342]
EXISTENCE:mutant phenotype:GO:0070525 tRNA threonylcarbamoyladenosine metabolic process [PMID:19287007|PMID:21183954]
Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:12615994]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:28987344]
EXISTENCE:mutant phenotype:GO:0035825 homologous recombination [PMID:28987344]
Protein with a role in DNA repair; mutants show reduced efficiency of non-homologous end-joining repair
ARS709; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:24124599|PMID:9092527]
EXISTENCE:direct assay:GO:0005388 P-type calcium transporter activity [PMID:10545175]
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:10545175]
EXISTENCE:direct assay:GO:0006816 calcium ion transport [PMID:10545175]
EXISTENCE:direct assay:GO:0006828 manganese ion transport [PMID:10801856]
EXISTENCE:direct assay:GO:0140613 P-type manganese transporter activity [PMID:10801856]
EXISTENCE:genetic interaction:GO:0006874 cellular calcium ion homeostasis [PMID:23569283]
EXISTENCE:genetic interaction:GO:0030026 cellular manganese ion homeostasis [PMID:29632074]
EXISTENCE:mutant phenotype:GO:0005388 P-type calcium transporter activity [PMID:10545175|PMID:1379856]
EXISTENCE:mutant phenotype:GO:0006816 calcium ion transport [PMID:10545175]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:29632074]
High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:2653812|PMID:2674688|PMID:3052856]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:3052856]
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:2068093]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2651899]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:3052856]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:2674688]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:29604179]
EXISTENCE:mutant phenotype:GO:0046688 response to copper ion [PMID:3043194]
Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12578832]
EXISTENCE:direct assay:GO:0030695 GTPase regulator activity [PMID:12578832]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:12578832]
RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12577053]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:10506208]
EXISTENCE:direct assay:GO:0015616 DNA translocase activity [PMID:10506208]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:10506208]
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:19129197]
EXISTENCE:direct assay:GO:0032392 DNA geometric change [PMID:10506208]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:11238918]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:12399378]
DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11248676|PMID:22842922]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:23872066]
EXISTENCE:genetic interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11401714]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:23223039]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:19793923]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11401714]
Zn(II)2Cys6 family transcription factor; positively regulates sterol uptake genes under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during vegetative growth; positively regulates mating with SUT2 by repressing expression of genes that act as mating inhibitors; repressed by STE12; relocalizes from the nucleus to the cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0097038 perinuclear endoplasmic reticulum [PMID:22586098]
EXISTENCE:genetic interaction:GO:0006915 apoptotic process [PMID:22586098]
EXISTENCE:mutant phenotype:GO:0016175 superoxide-generating NAD(P)H oxidase activity [PMID:22586098]
EXISTENCE:mutant phenotype:GO:0032956 regulation of actin cytoskeleton organization [PMID:22586098]
NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
hypothetical protein; deletion mutant has no detectable phenotype
ARS710; Autonomously Replicating Sequence
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
putative serine/threonine protein kinase RCK1
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:10778743]
Protein kinase involved in oxidative stress response; promotes pseudohyphal growth via activation of Ubp3p phosphorylation; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
carbonyl reductase (NADPH-dependent) ARI1
EXISTENCE:direct assay:GO:0004090 carbonyl reductase (NADPH) activity [PMID:19577617|PMID:19016485]
EXISTENCE:direct assay:GO:0016491 oxidoreductase activity [PMID:14574691]
NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:3281936]
EXISTENCE:direct assay:GO:0004559 alpha-mannosidase activity [PMID:3281936]
EXISTENCE:direct assay:GO:0034270 Cvt complex [PMID:12479808|PMID:20659891]
EXISTENCE:mutant phenotype:GO:0009313 oligosaccharide catabolic process [PMID:12723970]
EXISTENCE:mutant phenotype:GO:0019309 mannose catabolic process [PMID:26947009]
Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; upregulated under glucose starvation; targeted to vacuole via CVT pathway and AP-3 pathway
protein geranylgeranyltransferase type I subunit CDC43
EXISTENCE:direct assay:GO:0004662 CAAX-protein geranylgeranyltransferase activity [PMID:1400380]
EXISTENCE:direct assay:GO:0005953 CAAX-protein geranylgeranyltransferase complex [PMID:1400380]
EXISTENCE:direct assay:GO:0018344 protein geranylgeranylation [PMID:2034682]
Beta subunit of geranylgeranyltransferase type I; subunit of the Ram2p-Cdc43p heterodimer that catalyzes the geranylgeranylation of the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
EXISTENCE:direct assay:GO:0008897 holo-[acyl-carrier-protein] synthase activity [PMID:10320345]
EXISTENCE:mutant phenotype:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:3921525]
Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:9094717|PMID:9312008]
EXISTENCE:direct assay:GO:0016560 protein import into peroxisome matrix, docking [PMID:9094717]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831|PMID:12667447]
EXISTENCE:mutant phenotype:GO:0016560 protein import into peroxisome matrix, docking [PMID:16107331]
EXISTENCE:physical interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:9094717|PMID:9312008|PMID:16107331]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:9094717|PMID:9312008]
EXISTENCE:physical interaction:GO:0097027 ubiquitin-protein transferase activator activity [PMID:9094717]
Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9671481|PMID:22842922]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19368889]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:19368889]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9671481]
Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:10952318]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:10952318]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:10952318]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:10952318]
EXISTENCE:direct assay:GO:0019222 regulation of metabolic process [PMID:29462149]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:10361278]
EXISTENCE:direct assay:GO:0035065 regulation of histone acetylation [PMID:29462149]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:10952318]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:10952318]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23207916]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19620280]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:10952318]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:18258182]
EXISTENCE:mutant phenotype:GO:0032006 regulation of TOR signaling [PMID:29462149]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17681272]
Nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity; involved in positioning and spacing of nucleosomes over most genes independently of transcription; promotes nucleosome shifts in the 3' direction; has a role in modulating stress gene transcription
hypothetical protein; conserved among S. cerevisiae strains; YGL149W is not an essential gene
bifunctional chorismate synthase/riboflavin reductase [NAD(P)H] ARO2
EXISTENCE:direct assay:GO:0004107 chorismate synthase activity [PMID:8971708]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19793921|PMID:14562095]
EXISTENCE:direct assay:GO:0042602 riboflavin reductase (NADPH) activity [PMID:8971708]
EXISTENCE:mutant phenotype:GO:0004107 chorismate synthase activity [PMID:8971708]
EXISTENCE:mutant phenotype:GO:0009073 aromatic amino acid family biosynthetic process [PMID:8971708]
Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication
Integral membrane protein, member of the p24 family; forms complex with Erp3p, Erp5p, and Emp24p; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11739780]
EXISTENCE:direct assay:GO:0070939 Dsl1/NZR complex [PMID:19151722]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9639310]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:30837368]
EXISTENCE:physical interaction:GO:0070939 Dsl1/NZR complex [PMID:15958492]
Peripheral membrane protein required for COPI vesicle fusion to the ER; mediates Sey1p-independent homotypic ER fusion; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
ARS701; ARS containing only an imperfect match to the core consensus sequence
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25433290]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25433290]
EXISTENCE:direct assay:GO:0047372 acylglycerol lipase activity [PMID:25433290]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:25433290]
Lipase with specificity for monoacylglycerol; preferred substrate is 1-oleoylglycerol; null mutation affects lipid droplet morphology and overexpression causes increased accumulation of reactive oxygen species
EXISTENCE:direct assay:GO:0003747 translation release factor activity [PMID:10748224]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:8265342|PMID:14576278|PMID:16823961]
EXISTENCE:genetic interaction:GO:0003747 translation release factor activity [PMID:1475194]
EXISTENCE:genetic interaction:GO:0070126 mitochondrial translational termination [PMID:1475194]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:1475194]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:1475194]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:14734569]
EXISTENCE:mutant phenotype:GO:0070126 mitochondrial translational termination [PMID:15827612]
Mitochondrial translation release factor RF1; involved in stop codon recognition and hydrolysis of peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
putative glycosylphosphatidylinositol-alpha 1,2 mannosyltransferase
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:9639537|PMID:9576863]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:9759715]
Integral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:17190603]
EXISTENCE:direct assay:GO:0000502 proteasome complex [PMID:12408819]
EXISTENCE:direct assay:GO:0034450 ubiquitin-ubiquitin ligase activity [PMID:17190603]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25344756]
EXISTENCE:mutant phenotype:GO:0010994 free ubiquitin chain polymerization [PMID:22497224]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:17190603]
Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
ARS712; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
hypothetical protein; non-essential gene; contains multiple predicted transmembrane domains
putative flavin adenine dinucleotide transporter
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16717099]
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:27462707]
Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that arose from the whole genome duplication
hypothetical protein; has no significant sequence similarity to any known protein
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:21435344]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:7929113]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8001155]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:27068463]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17101773]
EXISTENCE:physical interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17101773]
Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
21S rRNA (uridine2791-2'-O) methyltransferase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11867542]
EXISTENCE:direct assay:GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity [PMID:11867542]
EXISTENCE:direct assay:GO:0031167 rRNA methylation [PMID:11867542]
EXISTENCE:mutant phenotype:GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity [PMID:14636587|PMID:11867542]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:11867542|PMID:14636587]
Mitochondrial 2' O-ribose methyltransferase; required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:31439809]
Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal; ribosomes containing Rpl1b are more efficient in translation of respiration-related proteins
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:9584169]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:9584169]
EXISTENCE:genetic interaction:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:9584169]
EXISTENCE:mutant phenotype:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:9584169]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:9584169]
Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12624196]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:14673157]
EXISTENCE:physical interaction:GO:0008623 CHRAC [PMID:14673157]
Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication
DNA-binding E3 ubiquitin-protein ligase SNT2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:23878396]
EXISTENCE:direct assay:GO:0016574 histone ubiquitination [PMID:22570702]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:22570702]
EXISTENCE:direct assay:GO:0070211 Snt2C complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:23878396]
EXISTENCE:mutant phenotype:GO:0036205 histone catabolic process [PMID:22570702]
Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitination, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:genetic interaction:GO:0031533 mRNA cap methyltransferase complex [PMID:9710603]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15226422]
EXISTENCE:mutant phenotype:GO:0004484 mRNA guanylyltransferase activity [PMID:8718687]
EXISTENCE:mutant phenotype:GO:0006370 7-methylguanosine mRNA capping [PMID:8718687]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:27348091]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15226422]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:9832501]
EXISTENCE:physical interaction:GO:0031533 mRNA cap methyltransferase complex [PMID:9710603]
Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant
mitochondrial 37S ribosomal protein RSM23
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
U2-type spliceosomal complex subunit CWC23
EXISTENCE:direct assay:GO:0005684 U2-type spliceosomal complex [PMID:11884590]
EXISTENCE:genetic interaction:GO:0000390 spliceosomal complex disassembly [PMID:19822657]
Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:19057509]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:15356001|PMID:15477388]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:23390378]
EXISTENCE:physical interaction:GO:0006281 DNA repair [PMID:8849885]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:29526591|PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:7706223]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:22927826]
EXISTENCE:mutant phenotype:GO:0055091 phospholipid homeostasis [PMID:33033181]
Protein required for normal ER membrane biosynthesis in response to ER stress; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; human homolog FIT2/FITM2, a fatty acyl-CoA diphosphatase, complements the yeast null mutant; disputed role in the synthesis of inositol phospholipids from inositol; required for inositol prototrophy
methylenetetrahydrofolate reductase (NAD(P)H) MET13
EXISTENCE:direct assay:GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity [PMID:10600168]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:10600168|PMID:10029993]
Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12364329]
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:24413168]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:20797862|PMID:24413168]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20797862]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12387888]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:24413168]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:24413168]
EXISTENCE:direct assay:GO:0035658 Mon1-Ccz1 complex [PMID:24413168]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:20797862|PMID:24413168]
EXISTENCE:genetic interaction:GO:0044395 protein targeting to vacuolar membrane [PMID:23979137]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:12387888]
EXISTENCE:mutant phenotype:GO:0044395 protein targeting to vacuolar membrane [PMID:23979137]
EXISTENCE:mutant phenotype:GO:0048278 vesicle docking [PMID:14662743]
EXISTENCE:physical interaction:GO:0035658 Mon1-Ccz1 complex [PMID:20797862]
Subunit of heterodimeric guanine nucleotide exchange factor (GEF); subunit of Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to vacuolar membrane; required for autophagy, CVT pathway, and mitophagy; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2517480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:2517480|PMID:3533916|PMID:385049]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:2517480]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0045903 positive regulation of translational fidelity [PMID:17545469|PMID:3522920]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:31182640]
Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; required for release of methyltransferase Bud23p from pre-40S precursor; homologous to mammalian ribosomal protein S2 and bacterial S5
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:26220998]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7962083|PMID:11779864|PMID:8474438]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7962083]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11927564]
EXISTENCE:direct assay:GO:0008097 5S rRNA binding [PMID:26220998]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:17630287]
EXISTENCE:direct assay:GO:0008312 7S RNA binding [PMID:26220998]
EXISTENCE:direct assay:GO:0033204 ribonuclease P RNA binding [PMID:26220998]
EXISTENCE:direct assay:GO:0045945 positive regulation of transcription by RNA polymerase III [PMID:26220998]
EXISTENCE:genetic interaction:GO:1900152 negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:26119729]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:11779864]
EXISTENCE:mutant phenotype:GO:0043488 regulation of mRNA stability [PMID:17636033]
EXISTENCE:physical interaction:GO:0043488 regulation of mRNA stability [PMID:17636033]
Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; stimulates RNA polymerase III transcription by enhancing TFIIIB binding to promoters; dimerizes upon RNA binding; protects mRNA against decay by nuclear exosome in a poly(A)-tail-dependent manner; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; NLS binds Kap104p; protein abundance increases under DNA replication stress; related to human hnRNPs
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:1905907 negative regulation of amyloid fibril formation [PMID:19129493]
Proposed gamma subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing
DEAH-box ATP-dependent RNA helicase PRP43
EXISTENCE:direct assay:GO:0000390 spliceosomal complex disassembly [PMID:16357217]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:17875666]
EXISTENCE:genetic interaction:GO:0000390 spliceosomal complex disassembly [PMID:17875666]
EXISTENCE:genetic interaction:GO:0030490 maturation of SSU-rRNA [PMID:19801658]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16382143]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:11886864]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:16227579|PMID:16382144]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:16382143]
EXISTENCE:mutant phenotype:GO:0071014 post-mRNA release spliceosomal complex [PMID:11886864]
RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11279158|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:18801050]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:27499294]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:27499294]
EXISTENCE:mutant phenotype:GO:0004672 protein kinase activity [PMID:22593570]
EXISTENCE:mutant phenotype:GO:0008289 lipid binding [PMID:27499294]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:27499294]
EXISTENCE:mutant phenotype:GO:1901006 ubiquinone-6 biosynthetic process [PMID:11279158|PMID:25498144]
ATPase required for ubiquinone biosynthesis and respiratory growth; maintains levels of CoQ biosynthetic proteins; binds to CoQ biosynthesis intermediates; UbiB protein kinase-like family member that lacks canonical protein kinase activity; similar to prokaryotic proteins involved in ubiquinone biosynthesis; human homolog ADCK3 complements a coq8 null, is associated with CoQ and respiratory-chain deficiencies, and is mutated in autosomal-recessive cerebellar ataxia type 2
ARS714; Autonomously Replicating Sequence
Tryptophan tRNA (tRNA-Trp), predicted by tRNAscan-SE analysis
hypothetical protein; conserved among S. cerevisiae strains; YGL118C is not an essential gene
ARS715; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
ubiquitin-protein transferase activating protein CDC20
EXISTENCE:direct assay:GO:0010997 anaphase-promoting complex binding [PMID:15916961]
EXISTENCE:direct assay:GO:0033597 mitotic checkpoint complex [PMID:11726501]
EXISTENCE:direct assay:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:15060174|PMID:17889668]
EXISTENCE:direct assay:GO:1990757 ubiquitin ligase activator activity [PMID:19362536|PMID:15060174]
EXISTENCE:genetic interaction:GO:1905786 positive regulation of anaphase-promoting complex-dependent catabolic process [PMID:9334304]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:12152084]
EXISTENCE:mutant phenotype:GO:0040020 regulation of meiotic nuclear division [PMID:10855492]
EXISTENCE:mutant phenotype:GO:0045842 positive regulation of mitotic metaphase/anaphase transition [PMID:10647015]
EXISTENCE:mutant phenotype:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:9504909]
EXISTENCE:mutant phenotype:GO:1905786 positive regulation of anaphase-promoting complex-dependent catabolic process [PMID:9334304]
EXISTENCE:physical interaction:GO:0005680 anaphase-promoting complex [PMID:9501986]
EXISTENCE:physical interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:9461437]
EXISTENCE:physical interaction:GO:0033597 mitotic checkpoint complex [PMID:10704439|PMID:11726501]
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
AMP-activated serine/threonine-protein kinase regulatory subunit SNF4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17237508|PMID:2481228]
EXISTENCE:direct assay:GO:0005641 nuclear envelope lumen [PMID:17237508]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17237508|PMID:2481228]
EXISTENCE:direct assay:GO:0031588 nucleotide-activated protein kinase complex [PMID:12393914|PMID:2481228]
EXISTENCE:genetic interaction:GO:0004679 AMP-activated protein kinase activity [PMID:2557546]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:2557546]
EXISTENCE:genetic interaction:GO:0043539 protein serine/threonine kinase activator activity [PMID:2557546]
EXISTENCE:mutant phenotype:GO:0004679 AMP-activated protein kinase activity [PMID:11486005|PMID:2557546|PMID:7913470]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:31962153]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:1355328]
EXISTENCE:mutant phenotype:GO:0043539 protein serine/threonine kinase activator activity [PMID:11486005|PMID:2557546]
EXISTENCE:mutant phenotype:GO:0045722 positive regulation of gluconeogenesis [PMID:2481228]
EXISTENCE:physical interaction:GO:0031588 nucleotide-activated protein kinase complex [PMID:2481228|PMID:9121458]
EXISTENCE:physical interaction:GO:0045722 positive regulation of gluconeogenesis [PMID:2481228]
Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
hypothetical protein; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:23746350]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11296242]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11296242]
EXISTENCE:mutant phenotype:GO:0006268 DNA unwinding involved in DNA replication [PMID:11296242]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:11296242]
EXISTENCE:physical interaction:GO:0031261 DNA replication preinitiation complex [PMID:11296242]
Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with the DDK-phosphorylated MCM complex and required for the recruitment of Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein
TATA-binding protein-associated factor TAF6
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10817999]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12840001]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:9844639]
EXISTENCE:mutant phenotype:GO:2000144 positive regulation of DNA-templated transcription initiation [PMID:29485702]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12628920]
EXISTENCE:mutant phenotype:GO:0072344 rescue of stalled ribosome [PMID:28757607]
Subunit of ribosome-associated quality control trigger complex (RQT); has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
hypothetical protein, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR binding [PMID:33876744]
EXISTENCE:direct assay:GO:0090615 mitochondrial mRNA processing [PMID:33876744]
EXISTENCE:direct assay:GO:1903108 regulation of mitochondrial transcription [PMID:33876744]
EXISTENCE:genetic interaction:GO:0006413 translational initiation [PMID:17194786]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:17194786|PMID:17194787]
EXISTENCE:mutant phenotype:GO:0043488 regulation of mRNA stability [PMID:17194787]
Mitochondrial protein that controls mitochondrial gene expression; binds the dodecamer element in mitochondrial mRNAs, facilitates processing and delivery of mitochondrial mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes; RMD9 has a paralog, YBR238C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:11082046]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:15210731]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15210731|PMID:10873803]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:10873803]
EXISTENCE:direct assay:GO:0031489 myosin V binding [PMID:9700160]
EXISTENCE:direct assay:GO:0031982 vesicle [PMID:12456647]
EXISTENCE:direct assay:GO:0120155 MIH complex [PMID:24895401]
EXISTENCE:genetic interaction:GO:1903476 protein localization to cell division site involved in mitotic actomyosin contractile ring assembly [PMID:10873803]
EXISTENCE:mutant phenotype:GO:0006903 vesicle targeting [PMID:12456647]
EXISTENCE:mutant phenotype:GO:1903476 protein localization to cell division site involved in mitotic actomyosin contractile ring assembly [PMID:10873803|PMID:11082046]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10873803|PMID:11082046|PMID:15210731]
EXISTENCE:physical interaction:GO:0016460 myosin II complex [PMID:15210731]
EXISTENCE:physical interaction:GO:0032038 myosin II heavy chain binding [PMID:15210731|PMID:11082046]
EXISTENCE:physical interaction:GO:0120155 MIH complex [PMID:24413167|PMID:24895401]
Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11726524|PMID:15710377]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:11069915]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:16343487]
EXISTENCE:direct assay:GO:0051351 positive regulation of ligase activity [PMID:11069915]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:16343487]
EXISTENCE:mutant phenotype:GO:0000049 tRNA binding [PMID:11726524]
EXISTENCE:mutant phenotype:GO:0006418 tRNA aminoacylation for protein translation [PMID:9659920]
EXISTENCE:mutant phenotype:GO:0017102 methionyl glutamyl tRNA synthetase complex [PMID:9659920]
Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p
Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family; VPS73 has a paralog, YBR241C, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:22096102]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:6337137]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:2104804]
Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance
EXISTENCE:direct assay:GO:0002953 5'-deoxynucleotidase activity [PMID:29752939]
EXISTENCE:direct assay:GO:0009159 deoxyribonucleoside monophosphate catabolic process [PMID:29752939]
EXISTENCE:direct assay:GO:0050484 GMP 5'-nucleotidase activity [PMID:29752939]
5'-deoxynucleotidase; non-essential gene involved in deoxyribonucleoside monophosphate degradation; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0006913 nucleocytoplasmic transport [PMID:10684247]
EXISTENCE:curator inference:GO:0017056 structural constituent of nuclear pore [PMID:11823431]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:18046406|PMID:11823431]
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:23974112]
Subunit of the Nup84 nuclear pore and SEACAT subcomplexes; involved in nucleocytoplasmic transport and NPC biogenesis in the nuclear pore subcomplex; subunit of SEACAT, a subcomplex of the SEA complex that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; human SEH1 homolog
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12773575]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:12773575]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:20670889]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:12773575]
Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at positions U1110, U2349, and U2351
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:12853481]
EXISTENCE:direct assay:GO:0098554 cytoplasmic side of endoplasmic reticulum membrane [PMID:12893879]
EXISTENCE:mutant phenotype:GO:0005484 SNAP receptor activity [PMID:12853481]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:12893879|PMID:12853481]
EXISTENCE:physical interaction:GO:0005484 SNAP receptor activity [PMID:12853481]
Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER and Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16365162]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1865879|PMID:11024003|PMID:1666302]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11024003]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:1865879]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0006407 rRNA export from nucleus [PMID:11739405]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:11024003]
EXISTENCE:mutant phenotype:GO:0006997 nucleus organization [PMID:8455603]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342]
Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12464632]
Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; can mutate to become a missense suppressor tRNA that inserts glutamate residues at lysine codons; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:7720726]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9624182]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:9335586]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:1493335]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:7628704]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:7720726]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:9650782]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:7628704]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10545112]
EXISTENCE:mutant phenotype:GO:0035543 positive regulation of SNARE complex assembly [PMID:11432826]
EXISTENCE:mutant phenotype:GO:0048210 Golgi vesicle fusion to target membrane [PMID:9650782]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:11432826]
EXISTENCE:physical interaction:GO:0000149 SNARE binding [PMID:16769821]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:10397773]
EXISTENCE:physical interaction:GO:0051082 unfolded protein binding [PMID:11432826]
Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment; mutation in human VPS45 is associated with congenital neutropenia and primary myelofibrosis of infancy
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:8550599]
EXISTENCE:direct assay:GO:0031251 PAN complex [PMID:8816488]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:11953437]
EXISTENCE:mutant phenotype:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:8550599]
EXISTENCE:mutant phenotype:GO:0004535 poly(A)-specific ribonuclease activity [PMID:8550599]
Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; complex acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19893618|PMID:27170178]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:19893618]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:19893618]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:19893618]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19893618|PMID:24402315]
EXISTENCE:physical interaction:GO:0034501 protein localization to kinetochore [PMID:24402315]
Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:8044840]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:11823431|PMID:18046406|PMID:9305650]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:20647373]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:20647373]
EXISTENCE:genetic interaction:GO:0036228 protein localization to nuclear inner membrane [PMID:21659568]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:8044840|PMID:9001245|PMID:9133678|PMID:9305650]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:22331846]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:10638763|PMID:11862215|PMID:16418532]
EXISTENCE:mutant phenotype:GO:0046822 regulation of nucleocytoplasmic transport [PMID:23499021]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:9001245|PMID:9133678|PMID:9305650|PMID:8195299]
Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8921898|PMID:15728363]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15728363]
EXISTENCE:direct assay:GO:0016226 iron-sulfur cluster assembly [PMID:17401378]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:26195633]
EXISTENCE:direct assay:GO:0051539 4 iron, 4 sulfur cluster binding [PMID:17401378]
EXISTENCE:direct assay:GO:1904564 Nbp35-Cfd1 ATPase complex [PMID:26195633]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0005506 iron ion binding [PMID:15728363]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:15728363]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:8921898]
Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10679327]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10679327]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11740566]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15342630]
EXISTENCE:physical interaction:GO:0032807 DNA ligase IV complex [PMID:17567543]
Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; sumoylated by Siz1p and Nfi1p SUMO ligases on Lys301; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
EXISTENCE:direct assay:GO:0000772 mating pheromone activity [PMID:6306574]
EXISTENCE:direct assay:GO:1903135 cupric ion binding [PMID:27476515]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3887136]
EXISTENCE:mutant phenotype:GO:0000772 mating pheromone activity [PMID:3887136]
Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed; binds copper(II) ions
hypothetical protein; conserved across S. cerevisiae strains; partially overlaps snR10, a snoRNA required for preRNA processing
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689|PMID:8455623]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:3327689]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:3327689]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9160748]
EXISTENCE:physical interaction:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:physical interaction:GO:0005730 nucleolus [PMID:2121740]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA); essential gene required for for preRNA processing, specifically for cleavage at sites A1 and A2; also guides pseudouridylation of large subunit (LSU) rRNA at position U2923
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:10089880]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:32265276]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:10089880]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10880451]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10880451]
EXISTENCE:direct assay:GO:0010994 free ubiquitin chain polymerization [PMID:19706603]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:10089880|PMID:9576943]
EXISTENCE:mutant phenotype:GO:0006301 postreplication repair [PMID:10089880|PMID:9576943]
EXISTENCE:mutant phenotype:GO:0044395 protein targeting to vacuolar membrane [PMID:32265276]
EXISTENCE:physical interaction:GO:0000209 protein polyubiquitination [PMID:10089880]
EXISTENCE:physical interaction:GO:0031371 ubiquitin conjugating enzyme complex [PMID:10089880]
Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
coiled-coil domain-containing protein MAD1
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19139260|PMID:24402315|PMID:27170178]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7593191]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12473689]
EXISTENCE:genetic interaction:GO:0006913 nucleocytoplasmic transport [PMID:12473689]
EXISTENCE:genetic interaction:GO:0044774 mitotic DNA integrity checkpoint signaling [PMID:16651657]
EXISTENCE:mutant phenotype:GO:0000776 kinetochore [PMID:19139260]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:10704439]
EXISTENCE:mutant phenotype:GO:0090268 activation of mitotic cell cycle spindle assembly checkpoint [PMID:15802513]
EXISTENCE:mutant phenotype:GO:1901925 negative regulation of protein import into nucleus during spindle assembly checkpoint [PMID:23177738]
EXISTENCE:physical interaction:GO:0006913 nucleocytoplasmic transport [PMID:12473689]
Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
hypothetical protein; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15813700|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15813700]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:16597698]
EXISTENCE:mutant phenotype:GO:0008374 O-acyltransferase activity [PMID:16597698|PMID:18036137]
EXISTENCE:mutant phenotype:GO:0015793 glycerol transmembrane transport [PMID:10931309]
EXISTENCE:mutant phenotype:GO:0019563 glycerol catabolic process [PMID:10747858]
Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; homolog of the mammalian Hedgehog pathway modulator HHATL; GUP1 has a paralog, GUP2, that arose from the whole genome duplication
ARS716; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
hypothetical protein; MINDY family deubiquitinase that does not appear to contain catalytic activity; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication; ortholog of human MINDY1/FAM63A
hypothetical protein; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22628554|PMID:22628558]
EXISTENCE:direct assay:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:25672363]
EXISTENCE:direct assay:GO:0050833 pyruvate transmembrane transporter activity [PMID:25672363]
EXISTENCE:mutant phenotype:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:22628554|PMID:22628558]
EXISTENCE:mutant phenotype:GO:0050833 pyruvate transmembrane transporter activity [PMID:22628554|PMID:22628558]
Highly conserved subunit of mitochondrial pyruvate carrier (MPC); MPC is a mitochondrial inner membrane complex that mediates pyruvate uptake and comprises Mpc1p and Mpc2p during fermentative growth, or Mcp1p and Mpc3p during respiratory growth; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog MPC1 is associated with lactic acidosis and hyperpyruvatemia
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9032262]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:22083961]
EXISTENCE:mutant phenotype:GO:0000464 endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9032262]
RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
EXISTENCE:mutant phenotype:GO:0015220 choline transmembrane transporter activity [PMID:2203793|PMID:3514579]
EXISTENCE:mutant phenotype:GO:0015871 choline transport [PMID:2203793]
EXISTENCE:mutant phenotype:GO:0031460 glycine betaine transport [PMID:16487344]
EXISTENCE:mutant phenotype:GO:0034228 ethanolamine transmembrane transporter activity [PMID:3514579]
EXISTENCE:mutant phenotype:GO:0034229 ethanolamine transport [PMID:3514579]
EXISTENCE:mutant phenotype:GO:1900749 (R)-carnitine transport [PMID:23755272]
EXISTENCE:mutant phenotype:GO:1901235 (R)-carnitine transmembrane transporter activity [PMID:23755272]
Plasma membrane transporter for choline, ethanolamine, and carnitine; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19466602]
EXISTENCE:direct assay:GO:0042273 ribosomal large subunit biogenesis [PMID:23945946]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:22893726]
Ribosomal 60S subunit protein L7A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:8674114]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A807
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position G805
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:32967926]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:10397772]
EXISTENCE:direct assay:GO:0005640 nuclear outer membrane [PMID:32059771]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10397772]
EXISTENCE:direct assay:GO:0034993 meiotic nuclear membrane microtubule tethering complex [PMID:32967926]
EXISTENCE:direct assay:GO:0044821 meiotic telomere tethering at nuclear periphery [PMID:32967926]
EXISTENCE:direct assay:GO:0140444 cytoskeleton-nuclear membrane anchor activity [PMID:32967926]
EXISTENCE:genetic interaction:GO:0030474 spindle pole body duplication [PMID:10654940]
EXISTENCE:mutant phenotype:GO:0000741 karyogamy [PMID:16923827]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:10654940|PMID:16923827|PMID:1869587]
EXISTENCE:mutant phenotype:GO:0034993 meiotic nuclear membrane microtubule tethering complex [PMID:32059771]
EXISTENCE:mutant phenotype:GO:0071988 protein localization to spindle pole body [PMID:16923827]
EXISTENCE:mutant phenotype:GO:0140444 cytoskeleton-nuclear membrane anchor activity [PMID:32059771]
Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication
stress-responsive transcription factor HSF1
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:3044612]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12821147]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:3044612]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:31124783]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:2017170|PMID:3044612|PMID:8985158]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:27320198|PMID:9296388]
EXISTENCE:mutant phenotype:GO:0070202 regulation of establishment of protein localization to chromosome [PMID:23447536]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:18270585]
Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:27402848|PMID:8670839]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:8670839]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11877447|PMID:22842922|PMID:16911514]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11877447|PMID:16911514|PMID:22842922]
EXISTENCE:direct assay:GO:0043515 kinetochore binding [PMID:16172405]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:14739928]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16172405]
EXISTENCE:mutant phenotype:GO:0032048 cardiolipin metabolic process [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:22157760]
EXISTENCE:mutant phenotype:GO:0034758 positive regulation of iron ion transport [PMID:7720713]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:22157760]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:32147528|PMID:12095998|PMID:7720713]
Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
DNA-directed RNA polymerase II core subunit RPB9
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:genetic interaction:GO:0001193 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II [PMID:17535246]
EXISTENCE:genetic interaction:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17030604]
EXISTENCE:mutant phenotype:GO:0001193 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II [PMID:16492753]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:17179178]
EXISTENCE:mutant phenotype:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:7800482]
RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription; positively regulates ATG1 transcription as a key regulator of autophagy
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:24675956]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:24675956]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:24675956]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15221522]
Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L7/L12 and human MRPL7 ribosomal proteins; associates with the mitochondrial nucleoid; required for normal respiratory growth
EXISTENCE:direct assay:GO:0000210 NAD+ diphosphatase activity [PMID:11361135]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:11361135]
EXISTENCE:direct assay:GO:0006734 NADH metabolic process [PMID:11361135]
EXISTENCE:mutant phenotype:GO:0110154 RNA decapping [PMID:34074215]
NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; enzymatic component of one of several partly redundant surveillance pathways involved in decapping of non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs); also possesses deCoAping activity involved in the decapping of non-canonical dephospho-CoA capped RNA (dpCoA-RNA); nudix hydrolase family member
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:17090597|PMID:12052880]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:18488019]
EXISTENCE:direct assay:GO:0046822 regulation of nucleocytoplasmic transport [PMID:31165730]
EXISTENCE:direct assay:GO:0071819 DUBm complex [PMID:20395473]
EXISTENCE:direct assay:GO:1905634 regulation of protein localization to chromatin [PMID:31165730]
EXISTENCE:genetic interaction:GO:0005198 structural molecule activity [PMID:15932941]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:15932941]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:15932941]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:17090597|PMID:15932941]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:18488019]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:20434206]
EXISTENCE:mutant phenotype:GO:0016573 histone acetylation [PMID:15932941]
EXISTENCE:mutant phenotype:GO:0016578 histone deubiquitination [PMID:19226466|PMID:25526805]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:17090597]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:19226466]
EXISTENCE:physical interaction:GO:0000124 SAGA complex [PMID:15932941]
EXISTENCE:physical interaction:GO:0046695 SLIK (SAGA-like) complex [PMID:15932941]
Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
EXISTENCE:direct assay:GO:0004378 GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity [PMID:16878994]
EXISTENCE:direct assay:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:16878994]
EXISTENCE:direct assay:GO:0033164 glycolipid 1,6-alpha-mannosyltransferase activity [PMID:16878994]
Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in N-linked glycosylation pathway; mutants exhibit temperature-sensitive growth and abnormal accumulation of lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; mutations of human ALG2 cause congenital myasthenic syndrome; human ALG2 complements temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12702275|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:24206665]
EXISTENCE:direct assay:GO:1990400 mitochondrial ribosomal large subunit rRNA binding [PMID:24206665]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:24206665]
EXISTENCE:mutant phenotype:GO:0016070 RNA metabolic process [PMID:12702275]
EXISTENCE:mutant phenotype:GO:1902775 mitochondrial large ribosomal subunit assembly [PMID:24206665]
Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17684231]
EXISTENCE:genetic interaction:GO:0009982 pseudouridine synthase activity [PMID:17684231]
EXISTENCE:genetic interaction:GO:0031119 tRNA pseudouridine synthesis [PMID:17684231]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:17684231]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:17684231]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25192136]
Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:196563|PMID:2665820]
EXISTENCE:mutant phenotype:GO:0004736 pyruvate carboxylase activity [PMID:2039506]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:8185321]
Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
ARS717; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:15664196|PMID:16777964]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:17620411]
EXISTENCE:direct assay:GO:0031116 positive regulation of microtubule polymerization [PMID:15664196]
EXISTENCE:direct assay:GO:0042729 DASH complex [PMID:15640796|PMID:15664196]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:17620411|PMID:20479465]
EXISTENCE:direct assay:GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore [PMID:20479465|PMID:20479468]
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p and Pse1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:18286174]
EXISTENCE:genetic interaction:GO:0007052 mitotic spindle organization [PMID:18286174]
Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:17918780|PMID:16319894]
EXISTENCE:genetic interaction:GO:0015976 carbon utilization [PMID:17918780]
EXISTENCE:mutant phenotype:GO:0004740 pyruvate dehydrogenase (acetyl-transferring) kinase activity [PMID:18180296]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:18180296]
EXISTENCE:mutant phenotype:GO:1901524 regulation of mitophagy [PMID:31548421]
EXISTENCE:mutant phenotype:GO:1904183 negative regulation of pyruvate dehydrogenase activity [PMID:18180296]
Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:14752010]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:9287349]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:3306404|PMID:9287349]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8436296|PMID:10880451]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:9287349]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:10848595]
EXISTENCE:direct assay:GO:0097505 Rad6-Rad18 complex [PMID:9287349]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:16783014]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:23988329]
EXISTENCE:genetic interaction:GO:0042275 error-free postreplication DNA repair [PMID:9576943]
EXISTENCE:genetic interaction:GO:0042276 error-prone translesion synthesis [PMID:770231]
EXISTENCE:genetic interaction:GO:0070987 error-free translesion synthesis [PMID:9409821]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:1651502|PMID:8436296]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:8436296|PMID:15504724]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:12226657]
EXISTENCE:mutant phenotype:GO:0010390 histone monoubiquitination [PMID:12535538|PMID:12535539]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:12535539|PMID:9343433]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:15632126|PMID:16166626]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:15280549]
EXISTENCE:mutant phenotype:GO:0070534 protein K63-linked ubiquitination [PMID:25622294]
EXISTENCE:mutant phenotype:GO:0071596 ubiquitin-dependent protein catabolic process via the N-end rule pathway [PMID:1651502|PMID:8505328]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:20462952]
EXISTENCE:mutant phenotype:GO:0090089 regulation of dipeptide transport [PMID:9427760]
EXISTENCE:mutant phenotype:GO:0120174 stress-induced homeostatically regulated protein degradation pathway [PMID:29861160]
EXISTENCE:mutant phenotype:GO:1990303 UBR1-RAD6 ubiquitin ligase complex [PMID:8505328]
EXISTENCE:physical interaction:GO:0006366 transcription by RNA polymerase II [PMID:15632065]
EXISTENCE:physical interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15504724]
EXISTENCE:physical interaction:GO:1990303 UBR1-RAD6 ubiquitin ligase complex [PMID:1651502|PMID:8505328]
EXISTENCE:physical interaction:GO:1990304 MUB1-RAD6-UBR2 ubiquitin ligase complex [PMID:18070918]
Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
hypothetical protein; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:genetic interaction:GO:0000920 septum digestion after cytokinesis [PMID:16041152]
Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12475963]
EXISTENCE:direct assay:GO:0045471 response to ethanol [PMID:31540996]
EXISTENCE:genetic interaction:GO:0009055 electron transfer activity [PMID:8530368]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:2202739]
EXISTENCE:mutant phenotype:GO:0004768 stearoyl-CoA 9-desaturase activity [PMID:1978720]
EXISTENCE:mutant phenotype:GO:0006636 unsaturated fatty acid biosynthetic process [PMID:1978720]
Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9732282]
EXISTENCE:direct assay:GO:0038024 cargo receptor activity [PMID:23270647]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9732282]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:9732282]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:9732282]
EXISTENCE:mutant phenotype:GO:0038024 cargo receptor activity [PMID:23270647]
COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p and Nha1p antiporter to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
pheromone-regulated DUP240 family protein PRM8
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12925749]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:12101299]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:12101299]
Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12925749|PMID:26928762]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12925749]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12925749]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12925749]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:12925749]
Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles; MST27 has a paralog, MST28, that arose from a segmental duplication
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0008175 tRNA methyltransferase activity [PMID:16642040]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0008175 tRNA methyltransferase activity [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:16642040]
EXISTENCE:mutant phenotype:GO:0031591 wybutosine biosynthetic process [PMID:16642040|PMID:17150819]
tRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:22851688|PMID:23222640]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:22851688]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:22851688]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:17908917|PMID:18981231|PMID:19470581|PMID:26777405]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:12861028]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:22851688]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:22851688]
EXISTENCE:mutant phenotype:GO:0016281 eukaryotic translation initiation factor 4F complex [PMID:8336723]
EXISTENCE:mutant phenotype:GO:0031369 translation initiation factor binding [PMID:22851688]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:18981231]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12861028]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:22851688]
Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); proposed to stimulate the ATPase and helicase activities of eIF4A synergistically with eIF4G based on sequence similarity with Tif4631p; proposed to be required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; associated with Pab1p-mediated poly(A)-tail stimulated, translation
proteasome regulatory particle base subunit RPT6
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10419517]
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:direct assay:GO:0019904 protein domain specific binding [PMID:7870180]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:genetic interaction:GO:0006289 nucleotide-excision repair [PMID:11410533]
EXISTENCE:genetic interaction:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:16269334]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:20855529]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:11389845]
EXISTENCE:mutant phenotype:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:17167105]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:19843524]
EXISTENCE:mutant phenotype:GO:0051091 positive regulation of DNA-binding transcription factor activity [PMID:16269334]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:22505030]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19412160]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:10688918|PMID:12447385]
EXISTENCE:physical interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:10688918]
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase catalytic subunit ALG13
EXISTENCE:direct assay:GO:0004577 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [PMID:16100113]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16100110|PMID:14690591]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762|PMID:16100113]
EXISTENCE:direct assay:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:16100113]
EXISTENCE:mutant phenotype:GO:0004577 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [PMID:15615718|PMID:16100113]
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:15615718|PMID:16100113]
EXISTENCE:physical interaction:GO:0043541 UDP-N-acetylglucosamine transferase complex [PMID:16100110|PMID:16100113]
Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant
EXISTENCE:direct assay:GO:0009898 cytoplasmic side of plasma membrane [PMID:20028738]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:31658248]
Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:17684230]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0072423 response to DNA damage checkpoint signaling [PMID:24603480]
EXISTENCE:physical interaction:GO:0005849 mRNA cleavage factor complex [PMID:11344258]
Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:15767671]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9334234]
EXISTENCE:direct assay:GO:0006362 transcription elongation by RNA polymerase I [PMID:7002153]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:17901206]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:7002153|PMID:7020755|PMID:8288647|PMID:9334234]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:18628399]
EXISTENCE:direct assay:GO:0031564 transcription antitermination [PMID:8636112|PMID:9037112|PMID:9169440]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:8876173]
EXISTENCE:direct assay:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:17913884]
EXISTENCE:genetic interaction:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:17901206]
EXISTENCE:genetic interaction:GO:0031440 regulation of mRNA 3'-end processing [PMID:15531585]
EXISTENCE:mutant phenotype:GO:0001139 RNA polymerase II complex recruiting activity [PMID:15767671]
EXISTENCE:mutant phenotype:GO:0001193 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II [PMID:14531857|PMID:16492753]
EXISTENCE:mutant phenotype:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:15767671|PMID:17901206]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:8288647]
EXISTENCE:mutant phenotype:GO:0031440 regulation of mRNA 3'-end processing [PMID:15531585]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:18628399]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:17913884]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:6985606|PMID:8876173]
General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
hypothetical protein; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
Putative mitochondrial hypothetical protein; conserved in fungi; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0004655 porphobilinogen synthase activity [PMID:2204246]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:8973546]
EXISTENCE:mutant phenotype:GO:0004655 porphobilinogen synthase activity [PMID:2445751|PMID:323256]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:14559249|PMID:323256]
Aminolevulinate dehydratase; a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus; human homolog ALAD can complement yeast hem2 mutant
ARS718; Autonomously Replicating Sequence
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:25656103]
EXISTENCE:direct assay:GO:0004090 carbonyl reductase (NADPH) activity [PMID:18630881|PMID:19016485]
EXISTENCE:direct assay:GO:0006725 cellular aromatic compound metabolic process [PMID:16268655]
EXISTENCE:direct assay:GO:0016614 oxidoreductase activity, acting on CH-OH group of donors [PMID:14574691|PMID:16268655]
EXISTENCE:direct assay:GO:0042180 cellular ketone metabolic process [PMID:14574691|PMID:16268655]
EXISTENCE:mutant phenotype:GO:1901426 response to furfural [PMID:25656103]
Aldehyde reductase; reduces aliphatic aldehyde substrates using NADH as cofactor; shown to reduce carbonyl compounds to chiral alcohols
initiation-specific alpha-1,6-mannosyltransferase
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:17042779|PMID:9276464]
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:10189369|PMID:1628616]
EXISTENCE:mutant phenotype:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:8253757]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:7649302]
Mannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12736687]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922|PMID:12736687]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:12736687]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:11901108]
EXISTENCE:mutant phenotype:GO:0008936 nicotinamidase activity [PMID:19381334]
EXISTENCE:mutant phenotype:GO:0019358 nicotinate nucleotide salvage [PMID:11901108]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11901108]
EXISTENCE:mutant phenotype:GO:1904524 negative regulation of DNA amplification [PMID:26195783]
Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YGL036W is not an essential gene
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12239282|PMID:8114729|PMID:9832517]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:7724528|PMID:9832517]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17237508|PMID:9285828|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17237508|PMID:11914276|PMID:9285828]
EXISTENCE:direct assay:GO:0061987 negative regulation of transcription from RNA polymerase II promoter by glucose [PMID:7724528]
EXISTENCE:genetic interaction:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:9832517]
EXISTENCE:genetic interaction:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517|PMID:7724528]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517]
Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
hypothetical protein; conserved among S. cerevisiae strains; YGL034C is not an essential gene
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9708739]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:9708739]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:16581767]
Meiosis-specific protein that localizes to chromosomes; prevents synapsis between nonhomologous chromosomes and ensures synapsis between homologs; forms complex with Mnd1p to promote homolog pairing and meiotic double-strand break repair; heterodimer of Hop2p-Mnd1p stimulates the Dmc1p-mediated strand invasion
EXISTENCE:direct assay:GO:0000752 agglutination involved in conjugation with cellular fusion [PMID:1756718]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:1756718]
EXISTENCE:direct assay:GO:0050839 cell adhesion molecule binding [PMID:11292808|PMID:7957044]
Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:30407570]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:30407570]
Ribosomal 60S subunit protein L24A; forms two bridges within ribosome, stimulates translation initiation and elongation; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:14970382]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9121443]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11142377]
EXISTENCE:genetic interaction:GO:0030627 pre-mRNA 5'-splice site binding [PMID:18570876]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9121443]
EXISTENCE:mutant phenotype:GO:0030627 pre-mRNA 5'-splice site binding [PMID:18570876]
EXISTENCE:mutant phenotype:GO:0048025 negative regulation of mRNA splicing, via spliceosome [PMID:9121443|PMID:18570876]
Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11116400]
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:11932453]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17443350]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:11116400]
Protein involved in nucleolar integrity and processing of pre-rRNA; has a role in processing rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; relocalizes from nucleus to nucleolus upon DNA replication stress
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
putative glucan endo-1,3-beta-D-glucosidase
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9748433]
Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
EXISTENCE:direct assay:GO:0004573 Glc3Man9GlcNAc2 oligosaccharide glucosidase activity [PMID:14680956]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8576053]
EXISTENCE:genetic interaction:GO:0070880 fungal-type cell wall beta-glucan biosynthetic process [PMID:9430631]
EXISTENCE:mutant phenotype:GO:0004573 Glc3Man9GlcNAc2 oligosaccharide glucosidase activity [PMID:9430631]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9363442]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:9430631]
Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
EXISTENCE:direct assay:GO:0004834 tryptophan synthase activity [PMID:391563|PMID:6342605]
EXISTENCE:mutant phenotype:GO:0000162 tryptophan biosynthetic process [PMID:10790693]
Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:23447536]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11073972]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10722672]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15254252]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12558798]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12482986]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:10722672]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:29698392]
EXISTENCE:direct assay:GO:1904263 positive regulation of TORC1 signaling [PMID:34535752]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:29698392]
EXISTENCE:mutant phenotype:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:29698392]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:34535752]
Phosphatidylinositol 3-phosphate binding protein; formation of a distinct complex with TORC1, induced by glutamine in dose-dependent manner, results in TORC1 activation, suggesting dual roles as both a glutamine sensor and a direct TORC1 activator; involved in leucine- and glutamine-responsive TORC1 activation, independent of the Gtr/Ego pathway; related to human PLEKHF1, a lysosomal membrane permeabilization regulator; contains a PtdIns3P binding FYVE domain; similar to Fab1p and Vps27p
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
EXISTENCE:curator inference:GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity [PMID:9405463]
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084]
Catalytic subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER lumen; involved in recognition of N-acetylglucosamine; forms a subcomplex with Ost3p and Ost4p
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16855400]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:33585466]
Atypical protein kinase; along with paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of formins and polarisome components in mitosis, and survival after spindle assembly checkpoint delay; regulates the morphogenesis checkpoint in response to polarity insults through inactivation of Mih1p; phosphorylated in response to DNA damage; similar to mammalian haspins
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:genetic interaction:GO:0043495 protein-membrane adaptor activity [PMID:18724936]
EXISTENCE:genetic interaction:GO:0045048 protein insertion into ER membrane [PMID:18724936]
EXISTENCE:mutant phenotype:GO:0000423 mitophagy [PMID:29673596]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:physical interaction:GO:0043529 GET complex [PMID:16269340]
Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
EXISTENCE:direct assay:GO:0005956 protein kinase CK2 complex [PMID:8135547]
EXISTENCE:direct assay:GO:0006356 regulation of transcription by RNA polymerase I [PMID:11551505]
EXISTENCE:direct assay:GO:0006359 regulation of transcription by RNA polymerase III [PMID:11551505]
EXISTENCE:direct assay:GO:0006974 cellular response to DNA damage stimulus [PMID:11551505]
EXISTENCE:direct assay:GO:0019887 protein kinase regulator activity [PMID:8135547]
EXISTENCE:direct assay:GO:0030291 protein serine/threonine kinase inhibitor activity [PMID:18265947]
EXISTENCE:direct assay:GO:0034456 UTP-C complex [PMID:17515605]
EXISTENCE:mutant phenotype:GO:0019887 protein kinase regulator activity [PMID:11827175]
Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:12756240]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11278728|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11171977|PMID:11273703]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:11278728]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:9813017]
EXISTENCE:mutant phenotype:GO:0051087 chaperone binding [PMID:11278728]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:11171977]
EXISTENCE:mutant phenotype:GO:0106034 protein maturation by [2Fe-2S] cluster transfer [PMID:11273703]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:11278728]
Specialized J-protein that functions in Fe-S cluster biogenesis; functions with Hsp70 in Fe-S cluster biogenesis in mitochondria; involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
EXISTENCE:direct assay:GO:0004057 arginyltransferase activity [PMID:2185248]
EXISTENCE:direct assay:GO:0016598 protein arginylation [PMID:2185248]
EXISTENCE:mutant phenotype:GO:0004057 arginyltransferase activity [PMID:2185248]
EXISTENCE:mutant phenotype:GO:0016598 protein arginylation [PMID:2185248]
Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway; may have a role in regulating stress response
EXISTENCE:direct assay:GO:0005049 nuclear export signal receptor activity [PMID:31023724]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:10525531]
EXISTENCE:direct assay:GO:0051168 nuclear export [PMID:31023724]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:10525531]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:10525531]
EXISTENCE:physical interaction:GO:0061608 nuclear import signal receptor activity [PMID:10525531]
Karyopherin beta; bidirectional nuclear transport receptor (biportin) that shuttles distinct sets of cargoes in opposite directions; responsible for import of Toa1p-Toa2p complex into nucleus; depletes eIF5A and eEF2 from cell nuclei; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:33634134]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:33634134]
EXISTENCE:direct assay:GO:0090339 negative regulation of formin-nucleated actin cable assembly [PMID:33634134]
EXISTENCE:direct assay:GO:0099503 secretory vesicle [PMID:33634134]
Formin inhibitor; negatively regulates Bnr1-mediated actin filament nucleation but not filament elongation; performs distinct but overlapping roles with Hof1p to control actin cable assembly and polarized secretion; bnr1 bil2 double mutants are inviable; localizes to the cytosol, secretory vesicles and sites of polarized growth
EXISTENCE:direct assay:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:17389596]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:18327269|PMID:15024427|PMID:23222640]
EXISTENCE:genetic interaction:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11101532]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:9150138]
Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
AMY1; ANT1; BOR2; CYH3; NRA2; SMR2; TIL1; TPE1; TPE3
drug-responsive transcription factor PDR1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11136452|PMID:22932476]
EXISTENCE:genetic interaction:GO:2001040 positive regulation of cellular response to drug [PMID:8078477]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8798494|PMID:9428726]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8798494|PMID:9428726]
EXISTENCE:mutant phenotype:GO:2001040 positive regulation of cellular response to drug [PMID:15713640|PMID:9767597]
Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000246 delta24(24-1) sterol reductase activity [PMID:14922]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10722850|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10722850]
EXISTENCE:direct assay:GO:0050614 delta24-sterol reductase activity [PMID:10722850]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:8125337]
C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
proteasome core particle subunit alpha 1
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:9087403]
EXISTENCE:curator inference:GO:0034515 proteasome storage granule [PMID:9087403]
EXISTENCE:curator inference:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:9087403]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019773 proteasome core complex, alpha-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
EXISTENCE:direct assay:GO:0001561 fatty acid alpha-oxidation [PMID:30530523]
EXISTENCE:direct assay:GO:0102672 fatty acid alpha-oxygenase activity [PMID:30530523]
EXISTENCE:mutant phenotype:GO:0046521 sphingoid catabolic process [PMID:25345524]
Dioxygenase that catalyzes alpha-oxygenation of 2-hydroxy fatty acids; involved in metabolism of phytosphingosine; not an essential gene
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:4355481]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:mutant phenotype:GO:0003861 3-isopropylmalate dehydratase activity [PMID:5724969]
EXISTENCE:mutant phenotype:GO:0009098 leucine biosynthetic process [PMID:5724969|PMID:6323436]
Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11739806|PMID:12469340|PMID:16622836]
EXISTENCE:direct assay:GO:0008553 P-type proton-exporting transporter activity [PMID:17912695]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:15536122]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:10716729]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:8396147]
EXISTENCE:direct assay:GO:1902600 proton transmembrane transport [PMID:8396147]
EXISTENCE:mutant phenotype:GO:1902906 proteasome storage granule assembly [PMID:23690178]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:29570051]
Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived hexameric protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding
hypothetical protein; conserved among S. cerevisiae strains; located in the upstream region of PMA1; deletion leads to polyamine resistance due to downregulation of PMA1
ARS719; Autonomously Replicating Sequence
hypothetical protein; identified by SAGE
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:24124599|PMID:7507493|PMID:26928762]
EXISTENCE:genetic interaction:GO:0005388 P-type calcium transporter activity [PMID:7507493]
EXISTENCE:mutant phenotype:GO:0005388 P-type calcium transporter activity [PMID:10431803]
EXISTENCE:mutant phenotype:GO:0006816 calcium ion transport [PMID:15280017|PMID:7507493]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:7507493]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:7507493]
Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in presence of elevated concentrations of calcium; targeted to vacuole via AP-3 pathway; similar to mammalian PMCA1a
Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19446323]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19446323]
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:19412159]
EXISTENCE:genetic interaction:GO:0070682 proteasome regulatory particle assembly [PMID:19412159|PMID:19446322|PMID:19446323]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:17499717]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19446323]
Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12456658]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12456658]
EXISTENCE:direct assay:GO:0030332 cyclin binding [PMID:11566880]
EXISTENCE:direct assay:GO:0045732 positive regulation of protein catabolic process [PMID:17178718]
EXISTENCE:direct assay:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:15060174]
EXISTENCE:direct assay:GO:2000060 positive regulation of ubiquitin-dependent protein catabolic process [PMID:18172166]
EXISTENCE:genetic interaction:GO:0010697 negative regulation of mitotic spindle pole body separation [PMID:16688214]
EXISTENCE:genetic interaction:GO:0120151 positive regulation of mitotic actomyosin contractile ring disassembly [PMID:19109423]
EXISTENCE:mutant phenotype:GO:0010697 negative regulation of mitotic spindle pole body separation [PMID:16688214]
EXISTENCE:mutant phenotype:GO:0045842 positive regulation of mitotic metaphase/anaphase transition [PMID:9831566]
EXISTENCE:mutant phenotype:GO:0120151 positive regulation of mitotic actomyosin contractile ring disassembly [PMID:19109423]
EXISTENCE:mutant phenotype:GO:1902426 deactivation of mitotic spindle assembly checkpoint [PMID:25025567]
EXISTENCE:physical interaction:GO:0005680 anaphase-promoting complex [PMID:9831566]
Activator of anaphase-promoting complex/cyclosome (APC/C); antagonist of the spindle assembly checkpoint; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including: Cdc20p, Ase1p, Cin8p, Fin1p and Clb5p; partially active in metaphase, and fully active in anaphase; cell-cycle regulated
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10359606]
Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating)
EXISTENCE:direct assay:GO:0000252 C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity [PMID:9811880]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11279045|PMID:26928762|PMID:12119386]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12119386]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:9811880]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:9811880]
EXISTENCE:mutant phenotype:GO:0047012 sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity [PMID:9811880]
C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:26545399]
EXISTENCE:direct assay:GO:0018023 peptidyl-lysine trimethylation [PMID:26545399]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:25446118]
EXISTENCE:mutant phenotype:GO:0018023 peptidyl-lysine trimethylation [PMID:25446118]
S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911|PMID:15353583]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18439903]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:15302830]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:14690608]
Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:12676951|PMID:16675952]
EXISTENCE:direct assay:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:16675952]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:17296727]
EXISTENCE:mutant phenotype:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:17296727]
Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:14617799]
EXISTENCE:genetic interaction:GO:1900063 regulation of peroxisome organization [PMID:27872254]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:14617799]
Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0001111 RNA polymerase II promoter clearance [PMID:7995524]
EXISTENCE:curator inference:GO:0001139 RNA polymerase II complex recruiting activity [PMID:7995524]
EXISTENCE:curator inference:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:7995524]
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:14749386]
EXISTENCE:direct assay:GO:0005674 transcription factor TFIIF complex [PMID:12242279|PMID:7995524]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:1331084|PMID:18362165]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:18362165]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:15572698|PMID:20033062]
TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
EXISTENCE:direct assay:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:12212850]
EXISTENCE:direct assay:GO:0000386 second spliceosomal transesterification activity [PMID:12212850]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:8474454]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:8474454]
EXISTENCE:direct assay:GO:0071021 U2-type post-spliceosomal complex [PMID:12212850]
EXISTENCE:genetic interaction:GO:0000386 second spliceosomal transesterification activity [PMID:17626844]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:14718167]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:12403466]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:14718167]
Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p
ethanolamine-phosphate cytidylyltransferase
EXISTENCE:direct assay:GO:0004306 ethanolamine-phosphate cytidylyltransferase activity [PMID:8982874]
EXISTENCE:mutant phenotype:GO:0004306 ethanolamine-phosphate cytidylyltransferase activity [PMID:8982874]
EXISTENCE:mutant phenotype:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:8982874]
Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2
ATPase-stabilizing factor family protein
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22442684]
EXISTENCE:mutant phenotype:GO:0071465 cellular response to desiccation [PMID:22442684]
Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication
ARS720; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:16339720]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9195974]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:12913108]
t-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functionally redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog
nicotinamide-nucleotide adenylyltransferase NMA2
EXISTENCE:direct assay:GO:0000309 nicotinamide-nucleotide adenylyltransferase activity [PMID:21621500]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11884393]
EXISTENCE:direct assay:GO:0009435 NAD biosynthetic process [PMID:21621500]
Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication
Putative cysteine synthase; localized to the mitochondrial outer membrane
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:9630245]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9630245]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:18591427]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:15256499]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:17627274]
EXISTENCE:genetic interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:17627274]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:2690082]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:15256499|PMID:18591427]
EXISTENCE:mutant phenotype:GO:0005034 osmosensor activity [PMID:12052881]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:12052881]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:12052881]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:19439450]
Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0016453 C-acetyltransferase activity [PMID:28356220]
Ethanol acetyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
Pyridoxamine-phosphate oxidase-related protein (PNPO-RP); deletion causes significant increase in 4.5.6-NADH3
hypothetical protein; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:23740823]
EXISTENCE:mutant phenotype:GO:0003867 4-aminobutyrate transaminase activity [PMID:3888627]
EXISTENCE:mutant phenotype:GO:0009450 gamma-aminobutyric acid catabolic process [PMID:3888627]
Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
H(+)-transporting V1 sector ATPase subunit F
Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
Putative mitochondrial hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27337501]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:20388713]
EXISTENCE:genetic interaction:GO:0042149 cellular response to glucose starvation [PMID:20388713]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10330137]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15637049|PMID:20388713]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:20388713]
Transmembrane sensor of the cell wall integrity pathway (CWI); involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; structurally and functionally similar to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0006400 tRNA modification [PMID:14633974]
EXISTENCE:direct assay:GO:0008193 tRNA guanylyltransferase activity [PMID:14633974]
tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
hypothetical protein; conserved across S. cerevisiae strains
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication
YGRWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:8226973|PMID:16407407]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:24821790]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:8226973]
Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:11493598]
EXISTENCE:direct assay:GO:0006879 cellular iron ion homeostasis [PMID:11493598]
EXISTENCE:direct assay:GO:0016971 flavin-linked sulfhydryl oxidase activity [PMID:10899311]
EXISTENCE:mutant phenotype:GO:0016972 thiol oxidase activity [PMID:15989955]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15989955]
EXISTENCE:mutant phenotype:GO:0045041 protein import into mitochondrial intermembrane space [PMID:15989955|PMID:16181637]
Flavin-linked sulfhydryl oxidase of the mitochondrial IMS; N-terminus is an intrinsically disordered domain that in the cytosol helps target Erv1p to mitochondria, and in the intermembrane space oxidizes Mia40p as part of a disulfide relay system that promotes intermembrane space retention of imported proteins; functional ortholog of human GFER (ALR); human GFER carrying N-terminal 21 amino acids of Erv1p functionally complements the lethality of the erv1 null mutation
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2865
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:26135751]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9620854]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:27156450]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:17881380]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9620854]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380]
Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:28465236]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:21525245|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0044255 cellular lipid metabolic process [PMID:28465236]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:28465236]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:28465236]
Conserved mitochondrial hypothetical protein; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18310357]
EXISTENCE:mutant phenotype:GO:0042546 cell wall biogenesis [PMID:18310357]
Protein involved in yeast cell wall biogenesis; localizes to the cell periphery; production of Nag1p is dependent upon the presence of Slt2p and Rlm1p; gene is nested within and antisense to IMO32
EXISTENCE:direct assay:GO:0000148 1,3-beta-D-glucan synthase complex [PMID:7983071]
EXISTENCE:direct assay:GO:0003843 1,3-beta-D-glucan synthase activity [PMID:7565718|PMID:7649185]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:15755916]
EXISTENCE:direct assay:GO:0006075 (1->3)-beta-D-glucan biosynthetic process [PMID:7565718]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:16116083]
EXISTENCE:mutant phenotype:GO:0006075 (1->3)-beta-D-glucan biosynthetic process [PMID:7565718]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:16116083]
Catalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239|PMID:14562095]
EXISTENCE:direct assay:GO:0005744 TIM23 mitochondrial import inner membrane translocase complex [PMID:15797382|PMID:15878866]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:15797382]
Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity
Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
hypothetical protein, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11504728]
EXISTENCE:direct assay:GO:0047874 dolichyldiphosphatase activity [PMID:11504728]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:10024662]
EXISTENCE:mutant phenotype:GO:0008610 lipid biosynthetic process [PMID:11504728]
Dolichyl pyrophosphate (Dol-P-P) phosphatase; cleaves the anhydride linkage in Dol-P-P; involved in synthesis of Dol-P-P-linked oligosaccharide intermediate required for protein N-glycosylation; has a luminally oriented active site in the ER; mutation affects vesicular transport, membrane and cell wall biogenesis and lipid homeostasis
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:20156967]
EXISTENCE:mutant phenotype:GO:0005576 extracellular region [PMID:20156967]
EXISTENCE:mutant phenotype:GO:0036042 long-chain fatty acyl-CoA binding [PMID:11294913]
EXISTENCE:mutant phenotype:GO:0042761 very long-chain fatty acid biosynthetic process [PMID:11294913]
Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20182505|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:genetic interaction:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:20182505]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:12093374]
EXISTENCE:mutant phenotype:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:20182505]
EXISTENCE:physical interaction:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:20182505]
Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
YGRCTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
ARS721; Autonomously Replicating Sequence
YGRCTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; mutated in S288C
ARS722; Autonomously Replicating Sequence
hypothetical protein; conserved among S. cerevisiae strains; YGR039W is not an essential gene
mitogen-activated serine/threonine-protein kinase KSS1
EXISTENCE:direct assay:GO:0004707 MAP kinase activity [PMID:8668180|PMID:8918885]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18417610|PMID:7579701]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:27400980]
EXISTENCE:genetic interaction:GO:0010973 positive regulation of division septum assembly [PMID:27400980]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:11583629]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:9393860]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:8001818]
EXISTENCE:mutant phenotype:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:9744865]
Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
ARS723; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11514631|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:8657162]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:17581861]
Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:26966248]
EXISTENCE:direct assay:GO:0035861 site of double-strand break [PMID:26966248]
EXISTENCE:direct assay:GO:0051096 positive regulation of helicase activity [PMID:26966248]
EXISTENCE:mutant phenotype:GO:0032205 negative regulation of telomere maintenance [PMID:26966248]
hypothetical protein; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
sedoheptulose-7-phosphate:D-glyceraldehyde-3- phosphate transaldolase NQM1
EXISTENCE:direct assay:GO:0004801 transaldolase activity [PMID:18831051]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:25887987]
Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication
ARS724; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9580682]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9580682]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11713667|PMID:9580682|PMID:22842922]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12006664|PMID:14668363]
EXISTENCE:direct assay:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:11713667]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:8339935]
EXISTENCE:mutant phenotype:GO:0045835 negative regulation of meiotic nuclear division [PMID:3513021]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12006664]
EXISTENCE:mutant phenotype:GO:0051038 negative regulation of transcription involved in meiotic cell cycle [PMID:8339935]
Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
putative phosphatidate cytidylyltransferase
EXISTENCE:direct assay:GO:0004605 phosphatidate cytidylyltransferase activity [PMID:23623749]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:16790493|PMID:16943180]
EXISTENCE:genetic interaction:GO:0032049 cardiolipin biosynthetic process [PMID:20485265]
EXISTENCE:mutant phenotype:GO:0004605 phosphatidate cytidylyltransferase activity [PMID:23623749]
EXISTENCE:mutant phenotype:GO:0032049 cardiolipin biosynthetic process [PMID:19114592]
Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase); required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
transcription factor TFIIIC subunit TFC4
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:1474578|PMID:2002052|PMID:2180956|PMID:8387209]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:3633923|PMID:8464480|PMID:2183033]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2002052]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:8264649]
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11733065|PMID:26928762]
EXISTENCE:direct assay:GO:0006274 DNA replication termination [PMID:31509741]
EXISTENCE:direct assay:GO:0030894 replisome [PMID:31509741]
EXISTENCE:direct assay:GO:0031593 polyubiquitin modification-dependent protein binding [PMID:16004872]
EXISTENCE:direct assay:GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex [PMID:11598205|PMID:11733065]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:16004872]
EXISTENCE:direct assay:GO:1990112 RQC complex [PMID:23178123]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11739805]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:11740563|PMID:11813000]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:20206597]
EXISTENCE:mutant phenotype:GO:0051974 negative regulation of telomerase activity [PMID:26365526]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:18812321]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:11740563|PMID:11813000]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579]
EXISTENCE:mutant phenotype:GO:1900182 positive regulation of protein localization to nucleus [PMID:11598205|PMID:11733065]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23358411]
Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transcription factors by regulated Ub/proteasome-dependent processing (RUP)
Mitochondrial outer membrane hypothetical protein; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication
hypothetical protein; mRNA identified as translated by ribosome profiling data
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:12133843]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:12133843]
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:12133843]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:12133843]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:12133843]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:12133843]
Eukaryotic initiation factor eIF2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
EXISTENCE:direct assay:GO:0000102 L-methionine secondary active transmembrane transporter activity [PMID:8893857]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25942624]
EXISTENCE:genetic interaction:GO:1903692 methionine import across plasma membrane [PMID:8893857]
EXISTENCE:mutant phenotype:GO:0000101 sulfur amino acid transport [PMID:8893857]
EXISTENCE:mutant phenotype:GO:1903692 methionine import across plasma membrane [PMID:8893857]
High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; involved in both methionine and cysteine uptake; subject to transport-activity dependent, methionine-induced endocytosis, vacuolar delivery and degradation; starvation-induced endocytosis is transport-activity independent
EXISTENCE:curator inference:GO:0006338 chromatin remodeling [PMID:10619019]
EXISTENCE:curator inference:GO:0008094 ATP-dependent activity, acting on DNA [PMID:10619019]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16024655]
EXISTENCE:physical interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:26387955]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26387955]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:26387955]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:26387955]
EXISTENCE:direct assay:GO:1990877 FNIP-folliculin RagC/D GAP [PMID:26387955]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:26387955]
Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface
EXISTENCE:direct assay:GO:0003400 regulation of COPII vesicle coating [PMID:22792405]
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:17640275]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22792405]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17640275]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:17640275]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:17640275]
EXISTENCE:mutant phenotype:GO:0003400 regulation of COPII vesicle coating [PMID:22792405]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:17640275]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:17640275]
EXISTENCE:physical interaction:GO:0003400 regulation of COPII vesicle coating [PMID:22792405]
Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:8636217]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18826657]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18826657|PMID:24390141]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:8636217]
Sporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at positions G2791 and G2793
EXISTENCE:direct assay:GO:0000254 C-4 methylsterol oxidase activity [PMID:8663358|PMID:12880870]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8663358]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8663358]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:8663358|PMID:12880870]
EXISTENCE:mutant phenotype:GO:0000254 C-4 methylsterol oxidase activity [PMID:8552601]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:8552601]
EXISTENCE:physical interaction:GO:0005783 endoplasmic reticulum [PMID:12119386]
C-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol; human MSMO1 functionally complements the growth defect caused by repression of ERG25 expression
phosphoribosylformylglycinamidine synthase
EXISTENCE:mutant phenotype:GO:0004642 phosphoribosylformylglycinamidine synthase activity [PMID:9331966]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:1743513]
Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11950926]
EXISTENCE:genetic interaction:GO:0032977 membrane insertase activity [PMID:17922846]
EXISTENCE:genetic interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926|PMID:17922846]
EXISTENCE:mutant phenotype:GO:0032977 membrane insertase activity [PMID:11950926]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926]
EXISTENCE:physical interaction:GO:0032977 membrane insertase activity [PMID:17452441]
EXISTENCE:physical interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:17452441]
Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functionally complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p
EXISTENCE:curator inference:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:16908835]
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:16908835]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:15057281]
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:26945063]
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:27376968]
EXISTENCE:direct assay:GO:0031934 mating-type region heterochromatin [PMID:15057281]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:26945063]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:16908835]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:26945063]
EXISTENCE:genetic interaction:GO:0006370 7-methylguanosine mRNA capping [PMID:12556496]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:20042611]
EXISTENCE:genetic interaction:GO:2001208 negative regulation of transcription elongation by RNA polymerase I [PMID:21467039]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:15057281]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:9450930]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:20713510]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:31462158]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:15057281]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:12554661]
EXISTENCE:mutant phenotype:GO:2000232 regulation of rRNA processing [PMID:21949810]
EXISTENCE:mutant phenotype:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:16908835]
EXISTENCE:physical interaction:GO:0032044 DSIF complex [PMID:9450930]
Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and along with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10373489]
EXISTENCE:direct assay:GO:0015225 biotin transmembrane transporter activity [PMID:10373489]
EXISTENCE:direct assay:GO:0015878 biotin transport [PMID:10373489]
High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
EXISTENCE:direct assay:GO:0006970 response to osmotic stress [PMID:31337681]
EXISTENCE:direct assay:GO:0030163 protein catabolic process [PMID:31337681]
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:31337681]
EXISTENCE:direct assay:GO:0042594 response to starvation [PMID:31337681]
Recognition component (N-recognin) of the Pro/N-degron pathway; recognizes and targets for degradation proteins with N-terminal degradation signals; expressed only under starvation or osmotic stress
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
hypothetical protein; contains a zinc finger motif similar to that of Adr1p
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane; SWAT-GFP and mCherry fusion proteins localize to the cytosol
Rho family guanine nucleotide exchange factor ROM1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22065638]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8641285]
EXISTENCE:genetic interaction:GO:0007264 small GTPase mediated signal transduction [PMID:8641285]
EXISTENCE:genetic interaction:GO:0090630 activation of GTPase activity [PMID:8641285]
Guanine nucleotide exchange factor (GEF) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:21912603]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:21912603]
Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9763508]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12196393]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12196393]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:9268361]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:18676617]
EXISTENCE:physical interaction:GO:0016567 protein ubiquitination [PMID:18676617]
Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9012791|PMID:9630245]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) [PMID:9582287]
EXISTENCE:mutant phenotype:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:9582287]
Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly
mitochondrial 54S ribosomal protein YmL25
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:12667447]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:10652355]
Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
tubulin-binding prefolding complex subunit PAC10
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9463374]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:16413483]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9611197]
EXISTENCE:physical interaction:GO:0016272 prefoldin complex [PMID:9463374]
Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding
hypothetical protein; YGR079W is not an essential gene
EXISTENCE:direct assay:GO:0003785 actin monomer binding [PMID:9700161]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:26458246]
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:16569665]
EXISTENCE:direct assay:GO:0030042 actin filament depolymerization [PMID:16569665|PMID:26458246]
EXISTENCE:direct assay:GO:0030836 positive regulation of actin filament depolymerization [PMID:26458246]
EXISTENCE:direct assay:GO:0042989 sequestering of actin monomers [PMID:9700161]
EXISTENCE:direct assay:GO:0051014 actin filament severing [PMID:16569665]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:16569665]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:16569665]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:16569665]
EXISTENCE:mutant phenotype:GO:0051014 actin filament severing [PMID:16569665]
Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, stimulates actin depolymerization as does the mouse homolog, mTwf1
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:17018574]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:17018574]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:31067825]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17018574]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:22956913]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17018574]
Nuclear export receptor for exportin Crm1p; required for pre-rRNA processing and nuclear export of pre-40S pre-ribosomal particles; associates with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; binds G-quadruplex (G4) structures in DNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936|PMID:16407407]
EXISTENCE:direct assay:GO:0005742 mitochondrial outer membrane translocase complex [PMID:9774667]
EXISTENCE:direct assay:GO:0030943 mitochondrion targeting sequence binding [PMID:9252394]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:8132642]
EXISTENCE:mutant phenotype:GO:0016031 tRNA import into mitochondrion [PMID:7837265]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:8132642|PMID:8223428|PMID:9774667]
Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
translation initiation factor eIF2B subunit delta
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8506384]
EXISTENCE:direct assay:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8506384]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:8506384]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:8887689]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8506384]
EXISTENCE:genetic interaction:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:genetic interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705]
EXISTENCE:genetic interaction:GO:0006446 regulation of translational initiation [PMID:8164676|PMID:8887689]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:8164676|PMID:8887689]
EXISTENCE:genetic interaction:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8336705]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:2038326]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:9032257]
EXISTENCE:mutant phenotype:GO:0030234 enzyme regulator activity [PMID:9032257]
EXISTENCE:physical interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8506384]
EXISTENCE:physical interaction:GO:0006446 regulation of translational initiation [PMID:8887689]
Delta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
mitochondrial 37S ribosomal protein YmS-A
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552|PMID:9151978]
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:2277060]
Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:36162502|PMID:16407407]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836|PMID:22842922]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:22123866]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:16496001|PMID:22869600]
EXISTENCE:mutant phenotype:GO:0006469 negative regulation of protein kinase activity [PMID:15016821]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16496001]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:16496001]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:15016821]
EXISTENCE:mutant phenotype:GO:0070941 eisosome assembly [PMID:18296441|PMID:19037108]
EXISTENCE:mutant phenotype:GO:0097446 protein localization to eisosome filament [PMID:25811606]
Eisosome core component involved in endocytosis; mitochondria phosphoprotein that localizes to the outer membrane and is involved in maintaining mitochondrial morphology and mitophagy; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0004737 pyruvate decarboxylase activity [PMID:23423327]
EXISTENCE:genetic interaction:GO:0000949 aromatic amino acid family catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006559 L-phenylalanine catabolic process [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006569 tryptophan catabolic process [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0004737 pyruvate decarboxylase activity [PMID:1744053]
EXISTENCE:mutant phenotype:GO:0006067 ethanol metabolic process [PMID:1744053]
Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
EXISTENCE:direct assay:GO:0004096 catalase activity [PMID:19463791]
EXISTENCE:mutant phenotype:GO:0000302 response to reactive oxygen species [PMID:15893481]
Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide
Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
ARS727; Autonomously Replicating Sequence
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
EXISTENCE:curator inference:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12150911|PMID:15590835|PMID:14562095]
EXISTENCE:direct assay:GO:0032545 CURI complex [PMID:17452446]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12837249]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:23194188]
Component of the small-subunit processome; required for nuclear export of tRNAs; forms a complex with Rrp7p; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
EXISTENCE:direct assay:GO:0000387 spliceosomal snRNP assembly [PMID:9199293]
EXISTENCE:mutant phenotype:GO:0000387 spliceosomal snRNP assembly [PMID:9199293]
Splicing factor; component of the U4/U6-U5 snRNP complex; homologous to human PRPF31 and used to study retinitis pigmentosa
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:8131744|PMID:21498574]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10984431|PMID:11434459]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10984431|PMID:11434459]
EXISTENCE:direct assay:GO:0034973 Sid2-Mob1 complex [PMID:9528782]
EXISTENCE:mutant phenotype:GO:0000280 nuclear division [PMID:2181271]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:22573892]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:18048916]
EXISTENCE:mutant phenotype:GO:0032465 regulation of cytokinesis [PMID:22573892]
EXISTENCE:mutant phenotype:GO:1901900 regulation of protein localization to cell division site [PMID:22573892]
EXISTENCE:physical interaction:GO:0034973 Sid2-Mob1 complex [PMID:9528782]
Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:24919400]
EXISTENCE:direct assay:GO:0061632 RNA lariat debranching enzyme activator activity [PMID:24919400]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:24919400]
Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0004832 valine-tRNA ligase activity [PMID:782885]
EXISTENCE:direct assay:GO:0006438 valyl-tRNA aminoacylation [PMID:782885]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:3275649]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:3275649]
Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
exosome non-catalytic core subunit RRP46
EXISTENCE:curator inference:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:10465791]
EXISTENCE:curator inference:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10465791]
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:19046973|PMID:10465791]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:19046973|PMID:10465791]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791]
EXISTENCE:mutant phenotype:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:19369424]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12411483]
EXISTENCE:direct assay:GO:0090422 thiamine pyrophosphate transmembrane transporter activity [PMID:12411483]
EXISTENCE:direct assay:GO:1990545 mitochondrial thiamine pyrophosphate transmembrane transport [PMID:12411483]
EXISTENCE:mutant phenotype:GO:1990545 mitochondrial thiamine pyrophosphate transmembrane transport [PMID:12411483]
Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19956799|PMID:14562095]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:14555478]
EXISTENCE:mutant phenotype:GO:0016247 channel regulator activity [PMID:24298058]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:14555478]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:14555478]
EXISTENCE:mutant phenotype:GO:0090372 positive regulation of glycerol transport [PMID:19956799]
Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme
EXISTENCE:direct assay:GO:0004197 cysteine-type endopeptidase activity [PMID:11081626|PMID:11081625]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11149918|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11149918|PMID:22842922]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:11149918]
EXISTENCE:genetic interaction:GO:0031536 positive regulation of exit from mitosis [PMID:14551257]
EXISTENCE:genetic interaction:GO:0032888 regulation of mitotic spindle elongation [PMID:18430955]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:9635435]
EXISTENCE:mutant phenotype:GO:0004197 cysteine-type endopeptidase activity [PMID:11081626]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:18321989]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:27418100|PMID:14551257]
EXISTENCE:mutant phenotype:GO:0032515 negative regulation of phosphoprotein phosphatase activity [PMID:16713564]
EXISTENCE:mutant phenotype:GO:0032888 regulation of mitotic spindle elongation [PMID:18430955]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
EXISTENCE:physical interaction:GO:0032515 negative regulation of phosphoprotein phosphatase activity [PMID:16713564]
Separase/separin, a caspase-like cysteine protease; cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit, resulting in the dissociation of cohesin from chromatin and sister chromatid separation; cleaves meiotic-cohesin subunit Rec8p along chromosome arms in meiosis I and at centromeric sites during meiosis II; inhibits PP2A-Cdc55p to promote mitotic exit; inhibited by Pds1p (securin); relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:9490802]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:10525964]
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:3513174]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:8649421]
EXISTENCE:mutant phenotype:GO:0034502 protein localization to chromosome [PMID:18334620]
Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10559187]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12807768]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:35134079]
EXISTENCE:genetic interaction:GO:0090630 activation of GTPase activity [PMID:15574876]
Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12774122]
EXISTENCE:mutant phenotype:GO:0004252 serine-type endopeptidase activity [PMID:12774122|PMID:20558178]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:12417197]
EXISTENCE:mutant phenotype:GO:0010821 regulation of mitochondrion organization [PMID:11907266|PMID:12707284]
Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
glutamyl-tRNA(Gln) amidotransferase subunit F
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19417106|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex [PMID:19417106]
EXISTENCE:direct assay:GO:0050567 glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity [PMID:19417106]
EXISTENCE:direct assay:GO:0070681 glutaminyl-tRNAGln biosynthesis via transamidation [PMID:19417106]
Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
mRNA-binding ribosome synthesis protein NOP7
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10684247]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614]
EXISTENCE:direct assay:GO:0042273 ribosomal large subunit biogenesis [PMID:11911362|PMID:16888624]
EXISTENCE:direct assay:GO:0070180 large ribosomal subunit rRNA binding [PMID:21811236]
EXISTENCE:direct assay:GO:0070545 PeBoW complex [PMID:16287855]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12837249]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:12110181]
EXISTENCE:physical interaction:GO:0070545 PeBoW complex [PMID:16287855]
Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8187178]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:8324825]
EXISTENCE:mutant phenotype:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:20048049]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15831453]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:23476016]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9660861]
EXISTENCE:mutant phenotype:GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly [PMID:9660861]
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:18799613]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:18799613]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:18799613]
ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; not essential for viability even though this is the only tRNA that decodes GAG codons
EXISTENCE:direct assay:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:1849458]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12972503|PMID:14562095|PMID:24223874]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12972503]
EXISTENCE:direct assay:GO:0060631 regulation of meiosis I [PMID:18423199]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:1387566]
EXISTENCE:genetic interaction:GO:0060631 regulation of meiosis I [PMID:24223874]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:1849458]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:1427070]
EXISTENCE:mutant phenotype:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:1849458]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:1427070]
EXISTENCE:mutant phenotype:GO:0008315 G2/MI transition of meiotic cell cycle [PMID:8455600]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:1849458]
EXISTENCE:mutant phenotype:GO:0060631 regulation of meiosis I [PMID:24223874]
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:14993267]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:14993267]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8253070|PMID:8319908]
EXISTENCE:genetic interaction:GO:0006279 premeiotic DNA replication [PMID:9732268]
EXISTENCE:genetic interaction:GO:0045740 positive regulation of DNA replication [PMID:8319908]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:14993267]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8253070]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:9732268]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:14993267]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:29263158]
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis
YGRWTy3-1; Ty3 element, LTR retrotransposon of the Gypsy (Metaviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; transposition is induced upon exposure to mating pheromone
EXISTENCE:direct assay:GO:0000943 retrotransposon nucleocapsid [PMID:15956549]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:21917850]
EXISTENCE:mutant phenotype:GO:0003677 DNA binding [PMID:21917850]
EXISTENCE:mutant phenotype:GO:0032197 transposition, RNA-mediated [PMID:17442718]
EXISTENCE:direct assay:GO:0004623 phospholipase A2 activity [PMID:19244244]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19244244]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:23637464]
EXISTENCE:direct assay:GO:0032048 cardiolipin metabolic process [PMID:19244244]
EXISTENCE:genetic interaction:GO:0032048 cardiolipin metabolic process [PMID:19244244]
EXISTENCE:mutant phenotype:GO:0004623 phospholipase A2 activity [PMID:19244244]
EXISTENCE:mutant phenotype:GO:0035965 cardiolipin acyl-chain remodeling [PMID:19244244|PMID:23637464]
Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
ARS728; Autonomously Replicating Sequence
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15306853|PMID:9162072]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15306853]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:17882259]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:11389896]
Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase; also localizes to the peroxisome
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:20479465|PMID:17620411]
EXISTENCE:direct assay:GO:0071459 protein localization to chromosome, centromeric region [PMID:30903360]
EXISTENCE:direct assay:GO:0098653 centromere clustering [PMID:30903360]
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p and Pse1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
chromatin-remodeling histone chaperone SPT6
EXISTENCE:direct assay:GO:0000414 regulation of histone H3-K36 methylation [PMID:18541663]
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:15531585]
EXISTENCE:direct assay:GO:0001073 transcription antitermination factor activity, DNA binding [PMID:15531585]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2201908]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:8633238]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:21094070]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:22308335]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:8633238]
EXISTENCE:genetic interaction:GO:0001073 transcription antitermination factor activity, DNA binding [PMID:15531585]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:8633238]
EXISTENCE:genetic interaction:GO:0031564 transcription antitermination [PMID:15531585]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16455495]
EXISTENCE:mutant phenotype:GO:0000414 regulation of histone H3-K36 methylation [PMID:18541663]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:6392016]
EXISTENCE:mutant phenotype:GO:0001073 transcription antitermination factor activity, DNA binding [PMID:15531585]
EXISTENCE:mutant phenotype:GO:0001178 regulation of transcriptional start site selection at RNA polymerase II promoter [PMID:12934008]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:12934008]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:8633238]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:19034519]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:21094070]
EXISTENCE:mutant phenotype:GO:0031564 transcription antitermination [PMID:15531585]
EXISTENCE:mutant phenotype:GO:0034728 nucleosome organization [PMID:21098123]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18086892]
EXISTENCE:mutant phenotype:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:20502685]
Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for fidelity of promoter selection through maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within coding regions; required for H3K36 trimethylation but not dimethylation by Set2p
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:genetic interaction:GO:0006450 regulation of translational fidelity [PMID:7498767|PMID:8415737|PMID:8950190]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:7498767|PMID:8415737|PMID:8950190]
Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:24574455|PMID:9813081|PMID:18046406]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327|PMID:17875746]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:9725905]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:14960378|PMID:9725905]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10512869]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20065092]
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:26172854]
EXISTENCE:direct assay:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:9234685]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:9234685]
Ammonium permease; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation; activity regulated by TORC1 effectors, Npr1p and Par32p; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922|PMID:14562095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15879523|PMID:16024810]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:17660569]
Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:12694636]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12694636]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12694636]
EXISTENCE:direct assay:GO:0006470 protein dephosphorylation [PMID:7925273]
Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
asparagine synthase (glutamine-hydrolyzing) 2
EXISTENCE:direct assay:GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity [PMID:6105958]
EXISTENCE:genetic interaction:GO:0006529 asparagine biosynthetic process [PMID:8951815]
Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:33704406]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:32776922]
EXISTENCE:mutant phenotype:GO:0034490 basic amino acid transmembrane import into vacuole [PMID:32776922]
EXISTENCE:mutant phenotype:GO:0034618 arginine binding [PMID:33704406]
Vacuolar membrane protein required for uptake and storage of arginine in nitrogen-replete cells; nitrogen starvation appears to inhibit Vsb1-dependent activity, resulting in mobilization of stored vacuolar arginine to the cytosol; arginine uptake appears to be driven by a V-ATPase mediated H+ gradient; member of the APC (amino acid-polyamine-organocation) transporter superfamily
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
Protein involved in heme trafficking to the nucleus; expression is regulated by Msn2p/Msn4p, indicating possible role in stress response; localizes to cytosol and peroxisomes
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:19370060]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:12794079]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:11105756|PMID:11842115]
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:11105756]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:11842115]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:11105756|PMID:11842115]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:11105756]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:11105756]
Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668|PMID:19269952]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:33443082]
EXISTENCE:mutant phenotype:GO:0097446 protein localization to eisosome filament [PMID:33443082]
Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:20581291]
hypothetical protein; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9259555|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10835343|PMID:9632789]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:22375831]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:10835343]
EXISTENCE:genetic interaction:GO:0007007 inner mitochondrial membrane organization [PMID:19221197]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:12882345]
EXISTENCE:mutant phenotype:GO:0007007 inner mitochondrial membrane organization [PMID:19221197]
EXISTENCE:mutant phenotype:GO:0045861 negative regulation of proteolysis [PMID:10207067]
EXISTENCE:mutant phenotype:GO:0070584 mitochondrion morphogenesis [PMID:19221197]
Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:17452527|PMID:22085930]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:1326082]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:17452527|PMID:24034674]
EXISTENCE:direct assay:GO:0051865 protein autoubiquitination [PMID:17452527]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:17452527]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:2568633]
EXISTENCE:mutant phenotype:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:17452527]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29519818]
Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:11733989]
Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation
proteasome core particle subunit alpha 3
EXISTENCE:mutant phenotype:GO:0080129 proteasome core complex assembly [PMID:14739934]
Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:23577202]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:23577202]
EXISTENCE:physical interaction:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:23577202]
Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12562762]
EXISTENCE:mutant phenotype:GO:0000296 spermine transport [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000297 spermine transmembrane transporter activity [PMID:11171066]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11171066]
Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:9407032]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:16381814]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:10097110]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:9407032]
EXISTENCE:direct assay:GO:0031518 CBF3 complex [PMID:1997204|PMID:8670864|PMID:8706132]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:12566427]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:16381814]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:16330709]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8486732]
Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
Vacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; targeted to vacuole via AP-3 pathway; VPS62 has a paralog, TDA6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:17101785]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22718905]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:17101785]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11053386|PMID:22718905]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:18833194]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:15701790]
EXISTENCE:mutant phenotype:GO:0006885 regulation of pH [PMID:11053386|PMID:15701790]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:12615067|PMID:22718905]
EXISTENCE:mutant phenotype:GO:0030307 positive regulation of cell growth [PMID:31649258]
EXISTENCE:mutant phenotype:GO:0034504 protein localization to nucleus [PMID:22718905]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:17101785]
EXISTENCE:mutant phenotype:GO:0140311 protein sequestering activity [PMID:31649258]
EXISTENCE:physical interaction:GO:0000149 SNARE binding [PMID:17101785]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:22718905]
EXISTENCE:physical interaction:GO:0006886 intracellular protein transport [PMID:22718905]
EXISTENCE:physical interaction:GO:0034504 protein localization to nucleus [PMID:22718905]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:22718905]
v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; preferentially expressed after severe ethanol stress, involved in clearance of denatured proteins caused by severe ethanol stress
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
beta-glucan synthesis-associated protein SKN1
EXISTENCE:direct assay:GO:0016020 membrane [PMID:7929594]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:20030721]
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:15792805]
EXISTENCE:mutant phenotype:GO:0006078 (1->6)-beta-D-glucan biosynthetic process [PMID:7929594]
EXISTENCE:mutant phenotype:GO:0015926 glucosidase activity [PMID:10601196]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:15792805]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:7929594]
Protein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication
ARS729; Autonomously Replicating Sequence
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15544818|PMID:16850348]
EXISTENCE:direct assay:GO:0008198 ferrous iron binding [PMID:22031445]
EXISTENCE:direct assay:GO:0052837 thiazole biosynthetic process [PMID:22031445]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:9367751]
EXISTENCE:mutant phenotype:GO:0009228 thiamine biosynthetic process [PMID:7941734]
Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835|PMID:14690591]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:24357410]
EXISTENCE:mutant phenotype:GO:0032040 small-subunit processome [PMID:24357410]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:19806183]
Component of the SSU; required for pre-18S rRNA processing, biogenesis of the small ribosomal subunit; interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD repeats, and has homology to Spb1p
hypothetical protein; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0017196 N-terminal peptidyl-methionine acetylation [PMID:8175741]
hypothetical protein; has an apparent role in acetylation of N-terminal methionine residues
Ribosomal 60S subunit protein L24B; forms two bridges within ribosome, stimulates translation initiation and elongation; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:27758859]
EXISTENCE:direct assay:GO:0106158 glycero-3-phosphocholine acyltransferase activity [PMID:27758859]
EXISTENCE:genetic interaction:GO:0090640 phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine [PMID:30514764]
EXISTENCE:mutant phenotype:GO:0036151 phosphatidylcholine acyl-chain remodeling [PMID:30514764]
Glycerophosphocholine acyltransferase (GPCAT); involved in in phosphatidylcholine (PC) synthesis; uses acyl-CoA to acylate glycero-3-phosphocholine to yield lyso-PC; also catalyzes acylation of glycerophosphoethanolamine with acyl-CoA; predicted to be an integral membrane protein
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:20124025|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:20124025]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:19751518]
EXISTENCE:mutant phenotype:GO:0000963 mitochondrial RNA processing [PMID:19562342]
EXISTENCE:mutant phenotype:GO:0008380 RNA splicing [PMID:19562342]
EXISTENCE:mutant phenotype:GO:0016072 rRNA metabolic process [PMID:20124025]
EXISTENCE:mutant phenotype:GO:2000234 positive regulation of rRNA processing [PMID:32152734]
Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:12058023]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:1910037]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:12058023]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8723349|PMID:12058023]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15282802]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:12058023]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:12058023]
EXISTENCE:genetic interaction:GO:0032507 maintenance of protein location in cell [PMID:14872283]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9744878]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:20587777]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:2065354]
EXISTENCE:mutant phenotype:GO:0007264 small GTPase mediated signal transduction [PMID:1910037]
EXISTENCE:mutant phenotype:GO:0035025 positive regulation of Rho protein signal transduction [PMID:14988726]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:12058023|PMID:14988726]
EXISTENCE:mutant phenotype:GO:2000114 regulation of establishment of cell polarity [PMID:31411940]
EXISTENCE:mutant phenotype:GO:2001108 positive regulation of Rho guanyl-nucleotide exchange factor activity [PMID:14988726]
Ras-type GTPase; required for bud site selection during both axial and bipolar budding, for morphological changes in response to mating pheromone, and for efficient cell fusion; recruits Cdc24p to the incipient bud site, activating its guanyl-nucleotide exchange factor activity and the Rho-like GTPase, Cdc42p; localizes to the plasma membrane, the incipient bud site, polarized growth sites, the cell division site and the vacuole membrane; Ras superfamily member similar to mammalian Rap GTPases
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:16709151]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16936141]
Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
EXISTENCE:direct assay:GO:0004122 cystathionine beta-synthase activity [PMID:10766767|PMID:11948191|PMID:19264153]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0006535 cysteine biosynthetic process from serine [PMID:11948191]
EXISTENCE:direct assay:GO:0070814 hydrogen sulfide biosynthetic process [PMID:19531479]
EXISTENCE:mutant phenotype:GO:0004122 cystathionine beta-synthase activity [PMID:10509018|PMID:8366024]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:22438835]
EXISTENCE:mutant phenotype:GO:0019343 cysteine biosynthetic process via cystathionine [PMID:10509018|PMID:8366024]
EXISTENCE:mutant phenotype:GO:0019346 transsulfuration [PMID:8366024]
Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria; human CBS can complement yeast null mutant
cleavage polyadenylation factor subunit PTI1
EXISTENCE:curator inference:GO:0003723 RNA binding [PMID:12819204]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095|PMID:24390141]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:12504017]
EXISTENCE:mutant phenotype:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:12773397]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:12773397]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:12504017]
Essential component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; relocalizes to the cytosol in response to hypoxia; similar to mammalian Cleavage-Stimulation Factor CstF-64
phosphatidylethanolamine N-methyltransferase
EXISTENCE:direct assay:GO:0004608 phosphatidylethanolamine N-methyltransferase activity [PMID:3066687|PMID:2445736]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:3066687|PMID:2445736]
EXISTENCE:genetic interaction:GO:0004608 phosphatidylethanolamine N-methyltransferase activity [PMID:2445736]
EXISTENCE:genetic interaction:GO:0006656 phosphatidylcholine biosynthetic process [PMID:2445736]
EXISTENCE:mutant phenotype:GO:0004608 phosphatidylethanolamine N-methyltransferase activity [PMID:3066687]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:23000174|PMID:3066687]
Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
EXISTENCE:curator inference:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:8534909]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:15935758|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10611222]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:11030620]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791|PMID:18007593]
EXISTENCE:mutant phenotype:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
EXISTENCE:mutant phenotype:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10611222]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:19861225]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1706724|PMID:11914276]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:1706724]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:1706724]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:7800479]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:33106247]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:33106247]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:33106247]
EXISTENCE:mutant phenotype:GO:0051880 G-quadruplex DNA binding [PMID:33106247]
Nucleolin, nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; binds to G-quadruplex DNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation
Putative component of the protein phosphatase type 2A complex
hypothetical protein; identified by sequence comparison with hemiascomycetous yeast species
YGRWTy2-2; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
YGRCTy1-3; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:18606994|PMID:21062831|PMID:24080224]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:12810920]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21925384]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:17908917|PMID:18981231|PMID:26777405]
EXISTENCE:direct assay:GO:1901195 positive regulation of formation of translation preinitiation complex [PMID:30281017]
EXISTENCE:direct assay:GO:1902280 regulation of RNA helicase activity [PMID:24080224]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:12810920]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:19806183]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:21925384]
Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); stimulates the ATPase and helicase activities of eIF4A synergistically with eIF4G; required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; association with Pab1p mediates poly(A) tail-stimulated translation; role in assembly of PET region in biogenesis of 60S ribosomal subunit
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15989961|PMID:26928762|PMID:23836928]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:18258182]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:26387955]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:16143306]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10388807|PMID:11073942]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10388807|PMID:11073942]
EXISTENCE:direct assay:GO:0031902 late endosome membrane [PMID:16732272]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:23716719]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18258182]
EXISTENCE:direct assay:GO:0071986 Ragulator complex [PMID:16732272]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:25046117]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:15989961]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:35158208]
EXISTENCE:mutant phenotype:GO:1903778 protein localization to vacuolar membrane [PMID:25046117]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353|PMID:26387955]
EXISTENCE:physical interaction:GO:0071986 Ragulator complex [PMID:15989961]
EXISTENCE:physical interaction:GO:1990131 Gtr1-Gtr2 GTPase complex [PMID:10388807|PMID:16143306]
Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4 homolog; subunit of EGO, a vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and esorting of Gap1p; activates transcription when chromatin bound; role in the oxidative stress response; activated by the Lst4p-Lst7p GAP complex; homolog of human RagC
mitochondrial 37S ribosomal protein MRPS35
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:17475775]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17475775]
EXISTENCE:genetic interaction:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:17475775]
EXISTENCE:genetic interaction:GO:0065003 protein-containing complex assembly [PMID:17475775]
EXISTENCE:physical interaction:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:17475775]
Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:27062026]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:16643280]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:21849475]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:27062026]
EXISTENCE:physical interaction:GO:0006897 endocytosis [PMID:16643280]
Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:26928762]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:28049721]
EXISTENCE:mutant phenotype:GO:1900063 regulation of peroxisome organization [PMID:28049721]
Regulator of peroxisome abundance; peroxisomal membrane protein, remote homolog to several curvature-generating human proteins; functionally interacts with vesicle-budding-inducing ADP-ribosylation factor Arf1p
ARS731; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11406626]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11406626]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:11406626]
EXISTENCE:direct assay:GO:0031119 tRNA pseudouridine synthesis [PMID:11406626]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:11406626]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:11406626]
tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26450372]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26450372]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:30208026]
EXISTENCE:mutant phenotype:GO:0002182 cytoplasmic translational elongation [PMID:30208026]
EXISTENCE:mutant phenotype:GO:0055072 iron ion homeostasis [PMID:26450372]
Ribosome-associated protein; required for global translational elongation after recovery from stationary phase due to starvation; crosslinks to the 25S rRNA near the A site that overlaps the GTPase activation center, and to tRNAs; possible role in the iron deprivation response; localizes to the nucleus and cytoplasm, with enhanced nuclear localization under iron-replete conditions; null mutant exhibits slow growth during iron deprivation; orthologous to human CCDC124
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20016005]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:6427211]
EXISTENCE:genetic interaction:GO:0004609 phosphatidylserine decarboxylase activity [PMID:14660568]
Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventually give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:2645139|PMID:16823961|PMID:14576278|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0004825 methionine-tRNA ligase activity [PMID:2645139]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:2645139]
EXISTENCE:mutant phenotype:GO:0006431 methionyl-tRNA aminoacylation [PMID:2645139]
Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:9724632]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:14557247|PMID:15659647]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:15659647]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:15659647]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:9724632]
EXISTENCE:mutant phenotype:GO:0048280 vesicle fusion with Golgi apparatus [PMID:12657649]
EXISTENCE:physical interaction:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:15659647]
Integral membrane protein; required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; human homolog YIPF5 can complement yeast yip1 mutant
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:21076151]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:23899355]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:23899355]
Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8063753|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:8063753]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:15759116|PMID:8063753]
Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
hypothetical protein; predicted to have a role in cell budding based on computational 'guilt by association' analysis
EXISTENCE:direct assay:GO:0004506 squalene monooxygenase activity [PMID:9450962|PMID:8358382]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9450962|PMID:26928762]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093|PMID:9450962]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:8358382]
EXISTENCE:mutant phenotype:GO:0004506 squalene monooxygenase activity [PMID:200835]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:200835]
Squalene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine; directed to the ER via the guided entry of tail-anchored proteins (GET) pathway; human SQLE functionally complements the lethality of the erg1 null mutation
EXISTENCE:direct assay:GO:0004026 alcohol O-acetyltransferase activity [PMID:9836419]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:18034159]
EXISTENCE:genetic interaction:GO:1901089 acetate ester metabolic process involved in fermentation [PMID:12957907]
EXISTENCE:mutant phenotype:GO:0008202 steroid metabolic process [PMID:10103065]
EXISTENCE:mutant phenotype:GO:0009636 response to toxic substance [PMID:18034159]
Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922|PMID:11914276]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:15082763]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:26777405]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:30982600]
EXISTENCE:genetic interaction:GO:1900365 positive regulation of mRNA polyadenylation [PMID:9819425]
EXISTENCE:mutant phenotype:GO:0043007 maintenance of rDNA [PMID:25073155]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:15082763]
EXISTENCE:mutant phenotype:GO:1900365 positive regulation of mRNA polyadenylation [PMID:9819425]
EXISTENCE:mutant phenotype:GO:1901524 regulation of mitophagy [PMID:30982600]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:30982600]
EXISTENCE:physical interaction:GO:0045727 positive regulation of translation [PMID:15082763]
EXISTENCE:physical interaction:GO:1900365 positive regulation of mRNA polyadenylation [PMID:9819425]
Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; regulates TORC1 signaling and autophagy; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; regulates expression of genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:10323865]
EXISTENCE:direct assay:GO:0000817 COMA complex [PMID:14633972]
EXISTENCE:mutant phenotype:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:19597337]
Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7
ribonucleotide-diphosphate reductase subunit RNR4
EXISTENCE:direct assay:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:10535923|PMID:10716984]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12732713|PMID:9315670|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12732713|PMID:22842922]
EXISTENCE:direct assay:GO:0005971 ribonucleoside-diphosphate reductase complex [PMID:10716984]
EXISTENCE:direct assay:GO:0009263 deoxyribonucleotide biosynthetic process [PMID:10535923|PMID:5459124]
EXISTENCE:genetic interaction:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:9315670|PMID:9315671]
EXISTENCE:mutant phenotype:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:16285741|PMID:9315671]
EXISTENCE:physical interaction:GO:0005971 ribonucleoside-diphosphate reductase complex [PMID:9315670]
Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:10051608|PMID:10397776]
EXISTENCE:direct assay:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:12221072]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10051608|PMID:10469659]
EXISTENCE:direct assay:GO:0140318 protein transporter activity [PMID:10051608|PMID:10469659]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10469659]
EXISTENCE:mutant phenotype:GO:0140318 protein transporter activity [PMID:10469659]
EXISTENCE:physical interaction:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:10051608]
Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
ubiquinol--cytochrome-c reductase subunit 9
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:1332881]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:1332881]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:1332881]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:8389362]
Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:20462952]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:20462952]
EXISTENCE:genetic interaction:GO:0005737 cytoplasm [PMID:20080635]
EXISTENCE:genetic interaction:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:20462952]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:10581257|PMID:2209542]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:10581257]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:20080635]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:23988329]
EXISTENCE:mutant phenotype:GO:0071596 ubiquitin-dependent protein catabolic process via the N-end rule pathway [PMID:2209542]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:19041308|PMID:20080635|PMID:20462952|PMID:22670231|P MID:27448207]
EXISTENCE:physical interaction:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:10688918]
EXISTENCE:physical interaction:GO:1904855 proteasome regulatory particle binding [PMID:10688918]
E3 Ub-ligase (N-recognin); recognizes and ubiquitinates N-end rule pathway substrates in complex with Rad6p; Roq1p-bound form accelerates degradation of misfolded and native ER membrane and cytosolic proteins as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; role in ER-associated protein degradation; modifies Cup9p to regulate peptide transport; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
EXISTENCE:direct assay:GO:0004831 tyrosine-tRNA ligase activity [PMID:8509419|PMID:782885]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11359929]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11359929]
EXISTENCE:direct assay:GO:0006437 tyrosyl-tRNA aminoacylation [PMID:8509419|PMID:782885]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:11359929]
Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functionally complements the heat sensitivity of a ts allele; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:11904169]
EXISTENCE:direct assay:GO:0072542 protein phosphatase activator activity [PMID:11904169]
EXISTENCE:direct assay:GO:0080163 regulation of protein serine/threonine phosphatase activity [PMID:11904169]
EXISTENCE:genetic interaction:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:15572698|PMID:16081422|PMID:16147988|PMID:7724527]
EXISTENCE:genetic interaction:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:11003641]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:15572698|PMID:16081422|PMID:18362165]
EXISTENCE:mutant phenotype:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:18362165]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:18362165]
TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
EXISTENCE:direct assay:GO:0061770 translation elongation factor binding [PMID:30876804]
EXISTENCE:mutant phenotype:GO:0061077 chaperone-mediated protein folding [PMID:30876804]
Chaperone protein for translation factor eEF2; involved in biogenesis of eukaryotic Elongation Factor 2 (Eft1p, Eft2p); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:16541024]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:7969164]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7969164]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:1651171]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:18081426]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:16357219|PMID:24402315]
EXISTENCE:mutant phenotype:GO:0034508 centromere complex assembly [PMID:22365852]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12769845]
Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10757808]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10757808|PMID:15781460]
EXISTENCE:direct assay:GO:0016757 glycosyltransferase activity [PMID:23919454]
EXISTENCE:genetic interaction:GO:0006037 cell wall chitin metabolic process [PMID:18694928]
EXISTENCE:genetic interaction:GO:0016757 glycosyltransferase activity [PMID:18694928]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0006037 cell wall chitin metabolic process [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0016757 glycosyltransferase activity [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:18694928]
Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
EXISTENCE:mutant phenotype:GO:0005291 high-affinity L-histidine transmembrane transporter activity [PMID:5447432]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:3905514]
EXISTENCE:mutant phenotype:GO:0006828 manganese ion transport [PMID:9790586]
EXISTENCE:mutant phenotype:GO:0015817 histidine transport [PMID:3905514|PMID:5447432]
High-affinity histidine permease; also involved in the transport of manganese ions
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) TDH3
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:25288528]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:18959479]
EXISTENCE:direct assay:GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity [PMID:3905788]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11158358|PMID:26928762]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11158358]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:expression pattern:GO:0006094 gluconeogenesis [PMID:3905788]
EXISTENCE:expression pattern:GO:0006096 glycolytic process [PMID:3905788]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17726063]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:27247412]
EXISTENCE:mutant phenotype:GO:0020037 heme binding [PMID:30012884]
EXISTENCE:mutant phenotype:GO:0072593 reactive oxygen species metabolic process [PMID:17726063]
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:2007123]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:2007123|PMID:7947791]
EXISTENCE:direct assay:GO:0006086 acetyl-CoA biosynthetic process from pyruvate [PMID:2007123|PMID:7947791]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:2007123]
EXISTENCE:mutant phenotype:GO:0006086 acetyl-CoA biosynthetic process from pyruvate [PMID:2007123]
EXISTENCE:physical interaction:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:7947791]
E3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core
EXISTENCE:direct assay:GO:0005998 xylulose catabolic process [PMID:10981687]
EXISTENCE:mutant phenotype:GO:0004856 xylulokinase activity [PMID:9595677]
Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
ARS737; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:32554806]
EXISTENCE:genetic interaction:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:12769863|PMID:12881429]
EXISTENCE:genetic interaction:GO:0071028 nuclear mRNA surveillance [PMID:11030620]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10508172|PMID:9390555]
EXISTENCE:mutant phenotype:GO:0016075 rRNA catabolic process [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172|PMID:10611222]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
hypothetical protein; required for survival upon exposure to K1 killer toxin
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20424846]
EXISTENCE:genetic interaction:GO:0061091 regulation of phospholipid translocation [PMID:27033517]
EXISTENCE:mutant phenotype:GO:0015931 nucleobase-containing compound transport [PMID:20424846]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:27033517]
Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17576801]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17576801|PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:19075114|PMID:14690591]
EXISTENCE:direct assay:GO:0006623 protein targeting to vacuole [PMID:17576801]
EXISTENCE:direct assay:GO:0006898 receptor-mediated endocytosis [PMID:17576801]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:17576801]
EXISTENCE:direct assay:GO:0072659 protein localization to plasma membrane [PMID:19075114]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17576801]
Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4p; is required, along with Efr3p, for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches at the plasma membrane; positively regulates Stt4p; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
dolichyl-phosphate-mannose-protein mannosyltransferase PMT6
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:9184828]
EXISTENCE:mutant phenotype:GO:0035269 protein O-linked mannosylation [PMID:18182384]
Protein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12139626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12139626]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:25960406]
EXISTENCE:direct assay:GO:0033588 elongator holoenzyme complex [PMID:11689709]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11689709]
Subunit of Elongator complex; binds to microtubules via conserved alkaline residues; has two seven-bladed WD40 beta propellers; Elongator complex is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
EXISTENCE:direct assay:GO:0004105 choline-phosphate cytidylyltransferase activity [PMID:2826147]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12200438]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12200438]
EXISTENCE:direct assay:GO:0006656 phosphatidylcholine biosynthetic process [PMID:10397762]
EXISTENCE:direct assay:GO:0006657 CDP-choline pathway [PMID:10397762]
EXISTENCE:direct assay:GO:0042564 NLS-dependent protein nuclear import complex [PMID:19141610]
Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; controls phosphatidylcholine homeostasis; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
EXISTENCE:direct assay:GO:0004792 thiosulfate sulfurtransferase activity [PMID:19864628]
EXISTENCE:direct assay:GO:0016311 dephosphorylation [PMID:19382206]
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:19382206]
Phosphatase with sequence similarity to Mih1p; member of the Cdc25p subfamily of tyrosine phosphatases and Arr2p, an arsenate reductase, based on the presence of a rhodanese-homology domain; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3
EXISTENCE:mutant phenotype:GO:0004329 formate-tetrahydrofolate ligase activity [PMID:8464869]
EXISTENCE:mutant phenotype:GO:0004477 methenyltetrahydrofolate cyclohydrolase activity [PMID:8464869]
EXISTENCE:mutant phenotype:GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity [PMID:8464869]
EXISTENCE:mutant phenotype:GO:0006760 folic acid-containing compound metabolic process [PMID:5563870]
EXISTENCE:mutant phenotype:GO:0009113 purine nucleobase biosynthetic process [PMID:8464869]
Cytoplasmic trifunctional enzyme; catalyzes sequential reactions 10-formyl-THF synthetase (EC 6.3.4.3), 5,10-methenyl-THF cyclohydrolase (EC 3.5.4.9), and 5,10-methylene-THF dehydrogenase (EC 1.5.1.5), to supply forms of activated one-carbon units required for biosynthesis of purines, histidine, methionine, pantothenic acid, the formyl group of mitochondrial fMet-tRNAfMet, and the methyl group of thymidylate; null mutation causes auxotrophy for adenine and histidine in rich medium
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:14997573]
ATP-binding hypothetical protein; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
ubiquitin-binding ESCRT-I subunit protein MVB12
EXISTENCE:curator inference:GO:0032509 endosome transport via multivesicular body sorting pathway [PMID:17145965]
EXISTENCE:direct assay:GO:0000813 ESCRT I complex [PMID:17135292|PMID:17145965]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17135292|PMID:17145965|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17145965|PMID:26928762]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:19380877]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17135292|PMID:17145965]
EXISTENCE:mutant phenotype:GO:0031333 negative regulation of protein-containing complex assembly [PMID:17145965]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0000813 ESCRT I complex [PMID:17135292|PMID:17145965]
ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
EXISTENCE:mutant phenotype:GO:0006564 L-serine biosynthetic process [PMID:1326413]
EXISTENCE:mutant phenotype:GO:0036424 L-phosphoserine phosphatase activity [PMID:1326413]
Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8603912]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9813082]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9813082]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:9657146]
EXISTENCE:direct assay:GO:0080058 protein deglutathionylation [PMID:20074363]
EXISTENCE:genetic interaction:GO:0000103 sulfate assimilation [PMID:2026619]
EXISTENCE:genetic interaction:GO:0006749 glutathione metabolic process [PMID:23242256]
EXISTENCE:genetic interaction:GO:0045454 cell redox homeostasis [PMID:22561702]
EXISTENCE:genetic interaction:GO:0080058 protein deglutathionylation [PMID:20074363]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:8603912|PMID:9015301]
EXISTENCE:mutant phenotype:GO:0080058 protein deglutathionylation [PMID:27708136]
Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system that protects cells from oxidative and reductive stress; subunit of the LMA1 complex, with Pbi2p, that promotes vacuole inheritance; cofactor for Tsa1p; required for ER-Golgi transport; facilitates mitochondrial import of small Tims (Tim9p, Tim10p, Tim13p) by maintaining them in reduced form along with Trx1p; component of a signal transduction redox relay in a light sensing pathway; abundance increases under DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9852145|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9852145|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:9852145]
EXISTENCE:physical interaction:GO:0061770 translation elongation factor binding [PMID:9852145]
Essential protein with two zinc fingers; present in nucleus of growing cells, relocates to cytoplasm in starved cells via a process mediated by Cpr1p; binds translation elongation factor eEF-1 (Tef1p); relative distribution to nucleus increases upon DNA replication stress; human ZPR1 gene can complement yeast by allowing growth during down-regulation of yeast zpr1
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15025559]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15025559]
EXISTENCE:direct assay:GO:0008080 N-acetyltransferase activity [PMID:15025559]
EXISTENCE:direct assay:GO:0009410 response to xenobiotic stimulus [PMID:15025559]
EXISTENCE:mutant phenotype:GO:0008080 N-acetyltransferase activity [PMID:15025559]
N-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22129104]
Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:8626693]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:8626693]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:8626693]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:8626693|PMID:9973221]
Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication
mitochondrial 37S ribosomal protein RSM27
phosphatidylinositol N-acetylglucosaminyltransferase
EXISTENCE:curator inference:GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity [PMID:9729469]
EXISTENCE:direct assay:GO:0006506 GPI anchor biosynthetic process [PMID:8910381]
EXISTENCE:genetic interaction:GO:0000506 glycosylphosphatidylinositol-N- acetylglucosaminyltransferase (GPI-GnT) complex [PMID:9729469]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:8910381]
Membrane protein involved in the synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1 are functional homologs
EXISTENCE:direct assay:GO:0005262 calcium channel activity [PMID:10958666]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10958666]
EXISTENCE:direct assay:GO:0006816 calcium ion transport [PMID:10958666]
EXISTENCE:mutant phenotype:GO:0005262 calcium channel activity [PMID:9343395]
EXISTENCE:mutant phenotype:GO:0006816 calcium ion transport [PMID:9343395]
Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19139260]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9891088]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:18573877]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030619 U1 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030620 U2 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030621 U4 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0030623 U5 snRNA binding [PMID:31189105]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:9857197]
EXISTENCE:genetic interaction:GO:0034501 protein localization to kinetochore [PMID:19139260]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11313466]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:9323132]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:9323132|PMID:33481703]
EXISTENCE:mutant phenotype:GO:0051168 nuclear export [PMID:31189105]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:9323132]
EXISTENCE:mutant phenotype:GO:0071528 tRNA re-export from nucleus [PMID:33074312]
Nuclear export factor, exportin; major karyopherin involved in export of proteins, RNAs, and ribosomal subunits from the nucleus
mitochondrial 54S ribosomal protein YmL9
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:1597181]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2060626]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:2060626]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11489916]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11489916|PMID:15282802]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11489916|PMID:15282802]
EXISTENCE:mutant phenotype:GO:1902413 negative regulation of mitotic cytokinesis [PMID:16816427]
Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:24231520]
EXISTENCE:direct assay:GO:0000395 mRNA 5'-splice site recognition [PMID:18084028]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0030627 pre-mRNA 5'-splice site binding [PMID:11453062|PMID:18084028|PMID:9430647]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:9430647]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:9430647]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:3555841]
EXISTENCE:mutant phenotype:GO:0034403 alignment of 3' and 5' splice sites of mRNA [PMID:9430647]
U5 spliceosomal RNA (U5 snRNA), long form; associated with spliceosome
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:2440583]
EXISTENCE:direct assay:GO:0030627 pre-mRNA 5'-splice site binding [PMID:18084028|PMID:9430647]
U5 spliceosomal RNA (U5 snRNA), short form; derived from SNR7-L by 3' end processing; associated with spliceosome
EXISTENCE:direct assay:GO:0000372 Group I intron splicing [PMID:18388132]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8389363]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8389363]
EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR binding [PMID:18388132]
EXISTENCE:direct assay:GO:0097157 pre-mRNA intronic binding [PMID:18388132]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:9341683]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:2548921]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:2548921]
EXISTENCE:mutant phenotype:GO:0090615 mitochondrial mRNA processing [PMID:9341683]
Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing; also stimulates efficient synthesis of Cox1p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:15155809]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15103325]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18769150]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:22704557]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:15155809|PMID:22704557|PMID:22753491]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:15155809]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:15103325|PMID:22704557|PMID:22753491]
EXISTENCE:mutant phenotype:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:22704557|PMID:22753491]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18769150]
EXISTENCE:mutant phenotype:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:22704557|PMID:22753491]
Phosphoinositide (PI) binding protein; involved in micronucleophagy; belongs to the PROPPIN family of proteins defined by a seven-bladed beta-propeller fold and FRRG motif required for PI binding; displays punctate cytoplasmic localization
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11113970]
EXISTENCE:mutant phenotype:GO:0045117 azole transmembrane transport [PMID:11113970]
EXISTENCE:mutant phenotype:GO:1901474 azole transmembrane transporter activity [PMID:11113970]
Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole
EXISTENCE:direct assay:GO:1904668 positive regulation of ubiquitin protein ligase activity [PMID:15797379|PMID:11114178]
EXISTENCE:direct assay:GO:1990757 ubiquitin ligase activator activity [PMID:15797379|PMID:11114178]
EXISTENCE:genetic interaction:GO:1903024 positive regulation of ascospore-type prospore membrane formation [PMID:32788308]
EXISTENCE:mutant phenotype:GO:0007127 meiosis I [PMID:11114178]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:23101628]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11114178]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
EXISTENCE:mutant phenotype:GO:0044778 meiotic DNA integrity checkpoint signaling [PMID:23101628]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:23101628]
EXISTENCE:mutant phenotype:GO:0045732 positive regulation of protein catabolic process [PMID:21118994]
EXISTENCE:mutant phenotype:GO:1905786 positive regulation of anaphase-promoting complex-dependent catabolic process [PMID:11114178]
EXISTENCE:physical interaction:GO:0005680 anaphase-promoting complex [PMID:11114178]
Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest
dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase
EXISTENCE:direct assay:GO:0106073 dolichyl pyrophosphate Glc2Man9GlcNAc2 alpha-1,2-glucosyltransferase activity [PMID:9597543]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:9597543]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9597543]
Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase; located in the ER; functions in pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1; human homolog ALG10B can complement yeast die2 null mutant
Small toxic protein; overexpression inhibits ergosterol biosynthesis; not required for normal growth, not conserved in related yeast species including S. paradoxus
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10206705]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10206705]
EXISTENCE:genetic interaction:GO:0007346 regulation of mitotic cell cycle [PMID:11410349]
EXISTENCE:genetic interaction:GO:0032995 regulation of fungal-type cell wall biogenesis [PMID:10206705]
EXISTENCE:mutant phenotype:GO:0007346 regulation of mitotic cell cycle [PMID:12823808]
EXISTENCE:mutant phenotype:GO:0032995 regulation of fungal-type cell wall biogenesis [PMID:10206705]
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
EXISTENCE:genetic interaction:GO:0051321 meiotic cell cycle [PMID:10564265]
EXISTENCE:genetic interaction:GO:1904750 negative regulation of protein localization to nucleolus [PMID:14551257]
hypothetical protein; FEAR network component that partially overlaps the function of SPO12 in promoting Cdc14p release from the nucleolus during early mitotic anaphase and mitotic exit; overexpression partially suppresses the meiotic defect of spo12; BNS1 has a paralog, SPO12, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:22375831]
EXISTENCE:direct assay:GO:1904855 proteasome regulatory particle binding [PMID:11029046]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19446322|PMID:19446323]
Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6
EXISTENCE:mutant phenotype:GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity [PMID:11069666|PMID:7939631]
EXISTENCE:mutant phenotype:GO:0006796 phosphate-containing compound metabolic process [PMID:4570606]
Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:15611069]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15611069]
EXISTENCE:direct assay:GO:0034599 cellular response to oxidative stress [PMID:8810268|PMID:9545281]
EXISTENCE:mutant phenotype:GO:0016966 nitric oxide reductase activity [PMID:10758168|PMID:15611069]
EXISTENCE:mutant phenotype:GO:0071218 cellular response to misfolded protein [PMID:27341336]
EXISTENCE:mutant phenotype:GO:1902170 cellular response to reactive nitrogen species [PMID:27341336]
Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:22009199|PMID:21987634]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:21987634|PMID:22009199]
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex; orthologous to human APOO and APOOL
Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9786949]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9786949]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9786949|PMID:19053807]
EXISTENCE:direct assay:GO:1990615 Kelch-containing formin regulatory complex [PMID:24828508]
EXISTENCE:genetic interaction:GO:0032465 regulation of cytokinesis [PMID:24828508]
EXISTENCE:genetic interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:24828508]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:9786949]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0060627 regulation of vesicle-mediated transport [PMID:24828508]
Protein that negatively regulates mitotic exit; forms a complex with Kel1p and Bud14p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:9864360]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9864360]
EXISTENCE:direct assay:GO:0051099 positive regulation of binding [PMID:17451428]
EXISTENCE:genetic interaction:GO:0016558 protein import into peroxisome matrix [PMID:9864360]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:26276932]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9864360]
Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558]
EXISTENCE:direct assay:GO:0005945 6-phosphofructokinase complex [PMID:4271029|PMID:6231961|PMID:21241708]
EXISTENCE:direct assay:GO:0006096 glycolytic process [PMID:3000145]
EXISTENCE:genetic interaction:GO:0003872 6-phosphofructokinase activity [PMID:3007939]
EXISTENCE:genetic interaction:GO:0006096 glycolytic process [PMID:2965996]
EXISTENCE:mutant phenotype:GO:0003872 6-phosphofructokinase activity [PMID:3007939]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:2965996]
EXISTENCE:mutant phenotype:GO:0051453 regulation of intracellular pH [PMID:24860096]
Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:33443082]
EXISTENCE:physical interaction:GO:0006897 endocytosis [PMID:9531549]
Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:22628558]
EXISTENCE:mutant phenotype:GO:0006850 mitochondrial pyruvate transmembrane transport [PMID:22628554]
EXISTENCE:mutant phenotype:GO:0050833 pyruvate transmembrane transporter activity [PMID:22628554]
Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress
ARS733; Autonomously Replicating Sequence
succinate--CoA ligase (GDP-forming) subunit beta
EXISTENCE:direct assay:GO:0004775 succinate-CoA ligase (ADP-forming) activity [PMID:9874242]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9874242|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006104 succinyl-CoA metabolic process [PMID:9874242]
EXISTENCE:mutant phenotype:GO:0004775 succinate-CoA ligase (ADP-forming) activity [PMID:9874242]
Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10704371]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:15107621]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:11160833]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:15107621]
Protein required for actin organization and passage through Start; highly conserved nuclear protein; required for actin cytoskeleton organization; plays a critical role in G1 events; binds Nap1p; involved in 60S ribosome biogenesis
transcription factor TFIIIB subunit BRF1
EXISTENCE:curator inference:GO:0000995 RNA polymerase III general transcription initiation factor activity [PMID:11387215|PMID:12930823|PMID:1458536|PMID:7995525]
EXISTENCE:direct assay:GO:0000126 transcription factor TFIIIB complex [PMID:1423590|PMID:1458536]
EXISTENCE:direct assay:GO:0000994 RNA polymerase III core binding [PMID:7995525|PMID:9689057]
EXISTENCE:direct assay:GO:0001156 TFIIIC-class transcription factor complex binding [PMID:12930823]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:9271407]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:15190063]
EXISTENCE:direct assay:GO:0070893 transposon integration [PMID:10882723]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11387215]
EXISTENCE:mutant phenotype:GO:0001112 DNA-templated transcription open complex formation [PMID:11387215]
EXISTENCE:mutant phenotype:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11387215|PMID:9218475|PMID:9710642]
EXISTENCE:physical interaction:GO:0000994 RNA polymerase III core binding [PMID:7995525]
EXISTENCE:physical interaction:GO:0001156 TFIIIC-class transcription factor complex binding [PMID:12930823]
TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome
2',3'-cyclic-nucleotide 3'-phosphodiesterase
EXISTENCE:direct assay:GO:0004113 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity [PMID:10734185]
EXISTENCE:direct assay:GO:0009187 cyclic nucleotide metabolic process [PMID:10734185]
EXISTENCE:mutant phenotype:GO:0004113 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity [PMID:10734185]
Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0009051 pentose-phosphate shunt, oxidative branch [PMID:15454531]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15454531]
EXISTENCE:genetic interaction:GO:0017057 6-phosphogluconolactonase activity [PMID:15454531]
6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16449570]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:9832522]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9832522]
Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
EXISTENCE:physical interaction:GO:2000134 negative regulation of G1/S transition of mitotic cell cycle [PMID:33480968]
RNA binding protein and negative regulator of START; binds mRNA of the G1 cyclin CLN3 to stress granules for translational repression under conditions of stress; recruits the cyclin-dependent protein kinase Cdc28p to stress granules when stressful conditions are relieved, resulting in the dissolution of the granules, and resumption of translation; localizes to stress granules induced by glucose deprivation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19159085|PMID:14690591|PMID:14562095]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:21941128]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:21941128|PMID:14562095|PMID:14690591]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:21941128]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21941128]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21941128]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:21941128]
Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9224714|PMID:9674426]
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:18039853]
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:31699900]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:8601308]
EXISTENCE:direct assay:GO:0010484 histone H3 acetyltransferase activity [PMID:18458063]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:20126658]
EXISTENCE:direct assay:GO:0140068 histone crotonyltransferase activity [PMID:31699900]
EXISTENCE:direct assay:GO:0140671 ADA complex [PMID:10490601|PMID:7862114]
EXISTENCE:genetic interaction:GO:0010484 histone H3 acetyltransferase activity [PMID:18458063]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:19822662]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:11867538]
EXISTENCE:mutant phenotype:GO:0010484 histone H3 acetyltransferase activity [PMID:18458063]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:19822662]
Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
proteasome core particle subunit alpha 5
Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
ARS734; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000015 phosphopyruvate hydratase complex [PMID:6282834]
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16565073]
EXISTENCE:direct assay:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:16565073]
EXISTENCE:mutant phenotype:GO:0004634 phosphopyruvate hydratase activity [PMID:6282834]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:6282834]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:16565073]
Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication
ARS735; Replication origin; identified in multiple array studies, confirmed by plasmid-based recombinational ARS assay
putative N,N-dimethylaniline monooxygenase COQ6
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12721307]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:21944752|PMID:9266513]
EXISTENCE:mutant phenotype:GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [PMID:21944752]
Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
phosphogluconate dehydrogenase (decarboxylating) GND2
EXISTENCE:mutant phenotype:GO:0004616 phosphogluconate dehydrogenase (decarboxylating) activity [PMID:1328471]
EXISTENCE:mutant phenotype:GO:0009051 pentose-phosphate shunt, oxidative branch [PMID:1328471]
6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12890866]
EXISTENCE:direct assay:GO:0030170 pyridoxal phosphate binding [PMID:24184947]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:16601688]
EXISTENCE:mutant phenotype:GO:0031921 pyridoxal phosphate transport [PMID:25637770]
Mitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5'-phosphate (PLP) transporter, important for delivery of the PLP cofactor to mitochondrial enzymes; involved in mitochondrial iron homeostasis
EXISTENCE:direct assay:GO:0000014 single-stranded DNA endodeoxyribonuclease activity [PMID:8247134]
EXISTENCE:direct assay:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:8855246]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:7926735]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:12110180]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:7926735]
Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
ETC4; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; remains predominantly localized to the nuclear periphery throughout cell cycle; located between divergently transcribed genes RAD2 and TNA1
EXISTENCE:mutant phenotype:GO:0046942 carboxylic acid transport [PMID:10869563]
EXISTENCE:mutant phenotype:GO:0046943 carboxylic acid transmembrane transporter activity [PMID:10869563]
EXISTENCE:mutant phenotype:GO:1903222 quinolinic acid transmembrane transport [PMID:23457190]
High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17895371]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:9335339]
EXISTENCE:mutant phenotype:GO:0030123 AP-3 adaptor complex [PMID:9250663]
Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
EXISTENCE:direct assay:GO:0000408 EKC/KEOPS complex [PMID:16564010|PMID:16874308]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:9305753|PMID:14519092]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:14519092]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:11452010]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:16564010]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:14519092]
EXISTENCE:mutant phenotype:GO:0070525 tRNA threonylcarbamoyladenosine metabolic process [PMID:21183954]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16874308]
Component of KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; provides the ATPase and GTPase activities of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human ortholog TP53RK
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:24868093|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:18034159]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18034159]
EXISTENCE:direct assay:GO:0034084 steryl deacetylase activity [PMID:18034159]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:18034159]
EXISTENCE:mutant phenotype:GO:0034084 steryl deacetylase activity [PMID:18034159]
Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER
EXISTENCE:direct assay:GO:0004825 methionine-tRNA ligase activity [PMID:3312199]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:3905796]
EXISTENCE:direct assay:GO:0006431 methionyl-tRNA aminoacylation [PMID:3312199]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26359986]
Methionyl-tRNA synthetase; forms a complex with Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which increases the catalytic efficiency of both synthetases; involved in the nuclear export of tRNAs; mutations in human ortholog MARS is associated with pediatric pulmonary alveolar proteinosis, interstitial lung and liver disease, and Charcot-Marie-Tooth disease; human MARS gene can complement the yeast null mutant
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340]
hypothetical protein; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:31323700]
EXISTENCE:mutant phenotype:GO:0003934 GTP cyclohydrolase I activity [PMID:8573145]
EXISTENCE:mutant phenotype:GO:0009396 folic acid-containing compound biosynthetic process [PMID:8573145]
GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesis; human homolog GCH1 is implicated in dopa-responsive dystonia (DRD), and can complement yeast null mutant
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11489916|PMID:11914276]
Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:34471130]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18493054|PMID:19581291]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:34471130]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:34471130]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:16079223|PMID:19581291]
EXISTENCE:direct assay:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:34471130]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18493054]
EXISTENCE:mutant phenotype:GO:0003682 chromatin binding [PMID:19581291]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:34471130]
EXISTENCE:mutant phenotype:GO:0006337 nucleosome disassembly [PMID:34471130]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:32079723]
EXISTENCE:mutant phenotype:GO:0042393 histone binding [PMID:19581291]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:19683497]
EXISTENCE:mutant phenotype:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:34471130]
EXISTENCE:mutant phenotype:GO:2000219 positive regulation of invasive growth in response to glucose limitation [PMID:18202364]
Chromatin segregase involved in DNA replication; ATPase activity, stimulated by S phase CDK-dependent phosphorylation, is coupled to nucleosome disassembly, promoting DNA replication; regulates cell-cycle-dependent histone gene expression; contains AAA+-ATPase domains and a non-canonical bromodomain; hexameric; relocalizes to the cytosol in response to hypoxia; human ortholog ATAD2 is a coregulator of oncogenic transcription factors and an oncogene overexpressed in cancers with poor prognosis
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:10922069|PMID:11438647]
Subunit of ribosome-associated quality control trigger complex (RQT); putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses; human homolog is ASCC3
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22588997|PMID:14562095]
EXISTENCE:direct assay:GO:0030685 nucleolar preribosome [PMID:29481617]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:22588997]
EXISTENCE:mutant phenotype:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:16510898]
EXISTENCE:mutant phenotype:GO:0071027 nuclear RNA surveillance [PMID:29481617]
Ribosome biogenesis factor required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
hypothetical protein; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity []
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10817999|PMID:10818000|PMID:12600943]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:8980232]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:11677244]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:15143185]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:9288741|PMID:9288742]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10713163|PMID:12600943|PMID:12840001]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:15143185|PMID:9844639]
TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia
EXISTENCE:curator inference:GO:0006338 chromatin remodeling [PMID:14729968]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:17188033]
EXISTENCE:direct assay:GO:0015616 DNA translocase activity [PMID:17188033]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:15506919|PMID:18644858]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:14729968]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:14729968]
Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10359084]
EXISTENCE:genetic interaction:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10716935]
EXISTENCE:genetic interaction:GO:0034415 tRNA 3'-trailer cleavage, exonucleolytic [PMID:18456844]
EXISTENCE:genetic interaction:GO:0034476 U5 snRNA 3'-end processing [PMID:10716935]
EXISTENCE:mutant phenotype:GO:0000175 3'-5'-exoribonuclease activity [PMID:10716935]
EXISTENCE:mutant phenotype:GO:0002107 generation of mature 3'-end of 5S rRNA generated by RNA polymerase III [PMID:10716935]
EXISTENCE:mutant phenotype:GO:0034415 tRNA 3'-trailer cleavage, exonucleolytic [PMID:18456844|PMID:10716935]
3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
putative pantetheine-phosphate adenylyltransferase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:direct assay:GO:1990143 CoA-synthesizing protein complex [PMID:23789928]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19266201]
Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; involved in histone acylation; probable pantetheine-phosphate adenylyltransferase (PPAT); PPAT catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT) and by human COASY
U2-type spliceosomal complex subunit CWC22
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:20956557]
Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:19129178]
EXISTENCE:direct assay:GO:0005773 vacuole [PMID:23222640]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9748433|PMID:15781460]
EXISTENCE:genetic interaction:GO:0000747 conjugation with cellular fusion [PMID:9748433]
Cell wall protein with similarity to glucanases; targeted to vacuole via AP-3 pathway; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:12107183]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12107183]
EXISTENCE:genetic interaction:GO:0010521 telomerase inhibitor activity [PMID:14977919]
EXISTENCE:genetic interaction:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:14977919]
EXISTENCE:mutant phenotype:GO:0000494 box C/D RNA 3'-end processing [PMID:12107183]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:24823796]
EXISTENCE:mutant phenotype:GO:0010521 telomerase inhibitor activity [PMID:14977919]
EXISTENCE:mutant phenotype:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:14977919]
EXISTENCE:physical interaction:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:14977919]
Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9575223]
EXISTENCE:direct assay:GO:0008559 ABC-type xenobiotic transporter activity [PMID:23270647]
EXISTENCE:direct assay:GO:0042908 xenobiotic transport [PMID:23270647]
EXISTENCE:mutant phenotype:GO:0008559 ABC-type xenobiotic transporter activity [PMID:23270647]
EXISTENCE:mutant phenotype:GO:0042908 xenobiotic transport [PMID:23270647]
Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR)
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:2509432|PMID:9748433]
EXISTENCE:direct assay:GO:0042973 glucan endo-1,3-beta-D-glucosidase activity [PMID:8458852]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:16986442]
EXISTENCE:mutant phenotype:GO:0042973 glucan endo-1,3-beta-D-glucosidase activity [PMID:7851411]
Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall
EXISTENCE:curator inference:GO:0032259 methylation [PMID:21858014|PMID:22650761]
Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11157978]
EXISTENCE:mutant phenotype:GO:0097020 COPII receptor activity [PMID:11711675]
Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20368619|PMID:11914276]
EXISTENCE:direct assay:GO:0005840 ribosome [PMID:17242366]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:25380751]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0005730 nucleolus [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:20368619]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:15456889]
EXISTENCE:mutant phenotype:GO:0006452 translational frameshifting [PMID:16607023]
EXISTENCE:mutant phenotype:GO:0051083 'de novo' cotranslational protein folding [PMID:11274393]
Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant
EXISTENCE:mutant phenotype:GO:0004076 biotin synthase activity [PMID:10525840]
EXISTENCE:mutant phenotype:GO:0009102 biotin biosynthetic process [PMID:10525840]
Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
EXISTENCE:direct assay:GO:0004574 oligo-1,6-glucosidase activity [PMID:20471265|PMID:15291818]
EXISTENCE:direct assay:GO:0004575 sucrose alpha-glucosidase activity [PMID:24649402]
EXISTENCE:genetic interaction:GO:0046352 disaccharide catabolic process [PMID:20471265]
EXISTENCE:mutant phenotype:GO:0046352 disaccharide catabolic process [PMID:20562106]
Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family
EXISTENCE:mutant phenotype:GO:0044262 cellular carbohydrate metabolic process [PMID:3025617]
MAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C
EXISTENCE:direct assay:GO:0005352 alpha-glucoside:proton symporter activity [PMID:12702465]
EXISTENCE:direct assay:GO:0015574 trehalose transmembrane transporter activity [PMID:12702465]
EXISTENCE:direct assay:GO:0015771 trehalose transport [PMID:12702465]
EXISTENCE:genetic interaction:GO:0015768 maltose transport [PMID:6371820]
EXISTENCE:mutant phenotype:GO:0005364 maltose:proton symporter activity [PMID:8594329|PMID:1999393]
EXISTENCE:mutant phenotype:GO:0015768 maltose transport [PMID:8594329]
High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
EXISTENCE:direct assay:GO:0004575 sucrose alpha-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0032450 maltose alpha-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0033934 glucan 1,4-alpha-maltotriohydrolase activity [PMID:20471265]
EXISTENCE:genetic interaction:GO:0000025 maltose catabolic process [PMID:20471265]
EXISTENCE:genetic interaction:GO:0005987 sucrose catabolic process [PMID:20471265]
Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to both the endoplasmic reticulum and vacuole
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
TEL07R; Telomeric region on the right arm of Chromosome VII; annotated components include an X element core sequence, X element combinatorial repeats, and a long Y' element; TEL07R does have telomeric repeats (TEL07R-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
ARS736; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
Y' element ATP-dependent helicase protein 1 copy 3
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9837911]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9837911]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p