Saccharomyces cerevisiae S288C chromosome VI, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006940.\n\nOn Apr 26, 2011 this sequence version replaced NC_001138.4.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL06L; Telomeric region on the left arm of Chromosome VI; composed of an X element core sequence, X element combinatorial repeats, a stretch of telomeric repeats, and a short Y' element
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol
hypothetical protein; down-regulated at low calcium levels; mCherry fusion protein localizes to the vacuole
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
Helicase-like protein encoded within the telomeric Y' element; induced by treatment with 8-methoxypsoralen and UVA irradiation; SWAT-GFP and mCherry fusion proteins localize to the nucleus
hypothetical protein; induced by treatment with 8-methoxypsoralen and UVA irradiation
ARS600; Subtelomeric Autonomously Replicating Sequence; originally published as ARS120
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
EXISTENCE:direct assay:GO:0018820 cyanamide hydratase activity [PMID:25847245]
EXISTENCE:mutant phenotype:GO:0018890 cyanamide metabolic process [PMID:25847245]
Cyanamide hydratase that detoxifies cyanamide; member of the HD domain metalloprotein superfamily; expression is induced over 100-fold by cyanamide and by SN2-type DNA alkylating agents such as MMS and DMA; induction decreased in rad6 and rad18 mutants; gene and protein are identical to DDI2 and Ddi2p
putative pyridoxal 5'-phosphate synthase
EXISTENCE:direct assay:GO:0008614 pyridoxine metabolic process [PMID:9791124]
hypothetical protein; nearly identical to Sno2p; expression is induced before the diauxic shift and also in the absence of thiamin
EXISTENCE:direct assay:GO:0036381 pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity [PMID:30498136]
EXISTENCE:genetic interaction:GO:0008615 pyridoxine biosynthetic process [PMID:30498136]
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:30498136]
Pyridoxal-5'-phosphate synthase; involved in thiamine and pyridoxine biosynthesis; member of a stationary phase-induced gene family where transcriptional induction precedes the diauxic shift; induced in the absence of thiamine in a Thi2/3p-dependent manner and repressed in its presence; forms a co-regulated gene pair with SNO3; paralog of SNZ1 and SNZ2
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:12777485]
Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family; expression induced in cells treated with the mycotoxin patulin
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0006865 amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0015171 amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10654085]
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:14697254]
EXISTENCE:mutant phenotype:GO:0015171 amino acid transmembrane transporter activity [PMID:14697254]
Low-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
ARS600.4; Strong autonomously replicating sequence
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0015254 glycerol channel activity [PMID:12832087]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:12832087]
Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol
EXISTENCE:genetic interaction:GO:0004371 glycerone kinase activity [PMID:12401799]
EXISTENCE:genetic interaction:GO:0061610 glycerol to glycerone phosphate metabolic process [PMID:22979944]
EXISTENCE:mutant phenotype:GO:0004371 glycerone kinase activity [PMID:12401799]
EXISTENCE:mutant phenotype:GO:0097237 cellular response to toxic substance [PMID:12401799]
Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:25673751]
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:25673751]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:25673751]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:25673751]
Zinc cluster transcription factor that regulates respiratory growth; binds to promoters of genes involved in respiration, gluconeogenesis, and the glyoxylate shunt; required for normal carbon source utilization and adaptation to pH, osmotic and ethanol stress especially during non-fermentative metabolism
hypothetical protein; SWAT-GFP fusion protein localizes to the cell periphery while mCherry fusion protein localizes to both the cell periphery and vacuole; YFL051C is not an essential gene
ARS601; Autonomously replicating sequence on Chromosome VI; overlaps ARS602, ARS601/ARS602 together appear to be activated during the second half of S phase in 32% of cell cycles
ARS602; Autonomously replicating sequence on Chromosome VI; overlaps ARS601, ARS601/ARS602 together appear to be activated during the second half of S phase in 32% of cell cycles
EXISTENCE:direct assay:GO:0015693 magnesium ion transport [PMID:12167543]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:9430719]
EXISTENCE:mutant phenotype:GO:0006812 cation transport [PMID:9430719]
EXISTENCE:mutant phenotype:GO:0022890 inorganic cation transmembrane transporter activity [PMID:9430719]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9430719]
Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition
EXISTENCE:curator inference:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18644858]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:16204215]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:16464826|PMID:18644858|PMID:8127913|PMID:16204215]
Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:12857885]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12857885]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:direct assay:GO:0097367 carbohydrate derivative binding [PMID:16439369]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12857885]
Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; forms a complex with Ssp120p that may function in trafficking plasma membrane glycoproteins through early secretory pathway; EMP47 has a paralog, EMP46, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0007264 small GTPase mediated signal transduction [PMID:11591390]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:11591390]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:22842922]
EXISTENCE:physical interaction:GO:0007264 small GTPase mediated signal transduction [PMID:11591390]
GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:32978391]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:25565209]
EXISTENCE:mutant phenotype:GO:2000214 regulation of proline metabolic process [PMID:32978391]
Regulator of mitochondrial proline metabolism; tethered with Put7p to inner mitochondrial membrane in large hetero-oligomeric complex, abundance of which is regulated by proline; involved in mitochondrial proline homeostasis and cellular redox balance; null exhibits pronounced defect in proline utilization, and can be functionally complemented by expression of human homolog MCUR1
EXISTENCE:direct assay:GO:0004615 phosphomannomutase activity [PMID:3288631]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:3905826]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen; human homolog PMM2 complements yeast mutants
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:16427015]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:16427015]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:15755922]
Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0044233 mitochondria-associated endoplasmic reticulum membrane [PMID:26001273]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:26001273]
Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10608875|PMID:24390141|PMID:23836928]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:genetic interaction:GO:0004322 ferroxidase activity [PMID:9413439]
EXISTENCE:mutant phenotype:GO:0006826 iron ion transport [PMID:9413439]
Multicopper oxidase; integral membrane protein with similarity to Fet3p; targeted to vacuole via AP-3 pathway; may have role in iron transport
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
Putative transporter; member of the sugar porter family; YFL040W is not an essential gene; may have a role in intracellular sterol transport
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:3967297|PMID:8163554]
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608|PMID:16299513]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:6217414]
EXISTENCE:direct assay:GO:0005884 actin filament [PMID:6217414]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:10911987]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:8245125]
EXISTENCE:direct assay:GO:0030476 ascospore wall assembly [PMID:17118118]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:3967297|PMID:6365930|PMID:8163554]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:3967297|PMID:6365930|PMID:8163554]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:10911987|PMID:15485911]
EXISTENCE:genetic interaction:GO:0009306 protein secretion [PMID:10793147]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:9864365]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:17287397]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:8978821]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8590801]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:10793147]
EXISTENCE:mutant phenotype:GO:1902404 mitotic actomyosin contractile ring contraction [PMID:9732290]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:10952318|PMID:24034245]
Actin, structural protein in cytoskeleton; involved in cell polarization, endocytosis, and other cytoskeletal functions; monomeric actin in the nucleus plays a role in INO80 chromatin remodeling
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10747087]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:18388317]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:20375281]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:3311726|PMID:9447979]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10747087]
EXISTENCE:direct assay:GO:0005795 Golgi stack [PMID:12802060]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:26906739]
EXISTENCE:direct assay:GO:0031410 cytoplasmic vesicle [PMID:23129774]
EXISTENCE:direct assay:GO:0035493 SNARE complex assembly [PMID:8603910]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:18388317]
EXISTENCE:genetic interaction:GO:0048194 Golgi vesicle budding [PMID:18388317]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:15689495|PMID:2504726|PMID:7593181]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:18388317]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20375281]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20375281]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:20059749]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:26195667]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:20059749]
EXISTENCE:mutant phenotype:GO:0035494 SNARE complex disassembly [PMID:9157884]
EXISTENCE:mutant phenotype:GO:0043549 regulation of kinase activity [PMID:26195667]
EXISTENCE:mutant phenotype:GO:0048211 Golgi vesicle docking [PMID:9545229]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
EXISTENCE:mutant phenotype:GO:0090114 COPII-coated vesicle budding [PMID:15689495]
EXISTENCE:mutant phenotype:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:22844259]
EXISTENCE:mutant phenotype:GO:1990261 pre-mRNA catabolic process [PMID:22844259]
EXISTENCE:physical interaction:GO:0000149 SNARE binding [PMID:18388317]
EXISTENCE:physical interaction:GO:0043549 regulation of kinase activity [PMID:26195667]
Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles
EXISTENCE:curator inference:GO:0000070 mitotic sister chromatid segregation [PMID:9488492]
EXISTENCE:curator inference:GO:0005828 kinetochore microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0005880 nuclear microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0005881 cytoplasmic microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0030473 nuclear migration along microtubule [PMID:9488492]
EXISTENCE:curator inference:GO:0045143 homologous chromosome segregation [PMID:9488492]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:9488492]
EXISTENCE:direct assay:GO:0045298 tubulin complex [PMID:9488492]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:12181356]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:8171338]
EXISTENCE:mutant phenotype:GO:0031122 cytoplasmic microtubule organization [PMID:8171338|PMID:12181356]
EXISTENCE:mutant phenotype:GO:0090307 mitotic spindle assembly [PMID:12181356|PMID:7806575]
EXISTENCE:physical interaction:GO:0045298 tubulin complex [PMID:9488492]
Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
EXISTENCE:direct assay:GO:0003896 DNA primase activity [PMID:26184436]
EXISTENCE:direct assay:GO:0003899 DNA-directed 5'-3' RNA polymerase activity [PMID:2426263|PMID:3517858]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:2426263|PMID:3517858]
EXISTENCE:direct assay:GO:0006269 DNA replication, synthesis of RNA primer [PMID:26184436]
EXISTENCE:direct assay:GO:0006390 mitochondrial transcription [PMID:21911502]
EXISTENCE:direct assay:GO:0034245 mitochondrial DNA-directed RNA polymerase complex [PMID:7929382]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:3517858]
EXISTENCE:mutant phenotype:GO:0006390 mitochondrial transcription [PMID:3517858]
Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11747810|PMID:12196508]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12196508]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11747810]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11747810|PMID:12196508]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:12196508]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:11747810]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:11747810|PMID:12196508]
EXISTENCE:mutant phenotype:GO:0001934 positive regulation of protein phosphorylation [PMID:12196508]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:12972564]
EXISTENCE:mutant phenotype:GO:0007163 establishment or maintenance of cell polarity [PMID:12196508]
Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:6814480]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:27478489]
Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; autoregulates splicing of its pre-m-RNA; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0016020 membrane [PMID:26658609]
EXISTENCE:direct assay:GO:0035650 AP-1 adaptor complex binding [PMID:26658609]
EXISTENCE:mutant phenotype:GO:0035652 clathrin-coated vesicle cargo loading [PMID:26658609]
Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk
ARS603; Highly-efficient autonomously replicating sequence on Chromosome VI; activated during the second half of S phase in 67% of cell cycles
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:9744870|PMID:9111339|PMID:24140345|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:20471941|PMID:23273919]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690612]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690612|PMID:14562095]
EXISTENCE:genetic interaction:GO:0010508 positive regulation of autophagy [PMID:25660547]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24140345]
EXISTENCE:genetic interaction:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:24140345]
EXISTENCE:genetic interaction:GO:1903452 positive regulation of G1 to G0 transition [PMID:23273919]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:20471941]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:25660547]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24140345]
EXISTENCE:mutant phenotype:GO:0051039 positive regulation of transcription involved in meiotic cell cycle [PMID:9111339]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:24140345]
EXISTENCE:mutant phenotype:GO:1901992 positive regulation of mitotic cell cycle phase transition [PMID:23861665]
EXISTENCE:mutant phenotype:GO:1903452 positive regulation of G1 to G0 transition [PMID:20471941|PMID:9744870]
Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:15141165|PMID:9077435]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8898193]
EXISTENCE:direct assay:GO:0010674 negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:15141165]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9077435]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:15141165|PMID:9077435]
EXISTENCE:mutant phenotype:GO:0010674 negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:15141165]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16582425]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9077435|PMID:8898193]
Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates unfolded protein response via UPRE binding and membrane biogenesis; translation initiation repressed under non-stress conditions by un-spliced intron that interacts with 5'-UTR; under ER stress Ire1p nuclease cleaves out inhibitory intron and triggers efficient Hac1p translation; two functional forms of Hac1p are produced; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0008453 alanine-glyoxylate transaminase activity [PMID:16226833]
EXISTENCE:direct assay:GO:0019265 glycine biosynthetic process, by transamination of glyoxylate [PMID:16226833]
EXISTENCE:mutant phenotype:GO:0008453 alanine-glyoxylate transaminase activity [PMID:14745783]
Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant
cyclin-dependent protein kinase-activating kinase CAK1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9819350]
EXISTENCE:direct assay:GO:0019912 cyclin-dependent protein kinase activating kinase activity [PMID:10373527|PMID:8752210|PMID:8752211|PMID:8781234]
EXISTENCE:genetic interaction:GO:0060633 negative regulation of transcription initiation by RNA polymerase II [PMID:19269370]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8752210|PMID:8752211]
EXISTENCE:mutant phenotype:GO:0019912 cyclin-dependent protein kinase activating kinase activity [PMID:10373527|PMID:8752210|PMID:8752211|PMID:8781234]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:11739722]
Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; required for premeiotic DNA synthesis, expression of early and middle sporulation specific genes and direct phosphorylation of the Smk1p MAPK to regulate spore morphogenesis
putative ATP-binding cassette family ATPase CAF16
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0030014 CCR4-NOT complex [PMID:11113136]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11113136]
Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12189143]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12189143]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:12189143]
GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport; localizes to the ER and peroxisomes
EXISTENCE:direct assay:GO:0000755 cytogamy [PMID:17369365]
EXISTENCE:direct assay:GO:0004934 mating-type alpha-factor pheromone receptor activity [PMID:2161538]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2839507]
EXISTENCE:genetic interaction:GO:0004934 mating-type alpha-factor pheromone receptor activity [PMID:9742115]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3037311]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:17369365]
EXISTENCE:mutant phenotype:GO:0004934 mating-type alpha-factor pheromone receptor activity [PMID:6360378|PMID:9742115]
EXISTENCE:mutant phenotype:GO:0030031 cell projection assembly [PMID:12378535]
Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8862519|PMID:26928762]
EXISTENCE:genetic interaction:GO:0006505 GPI anchor metabolic process [PMID:14734546]
EXISTENCE:genetic interaction:GO:0050185 phosphatidylinositol deacylase activity [PMID:14734546]
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:8862519]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8862519]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:8862519]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11673477]
EXISTENCE:mutant phenotype:GO:0034368 protein-lipid complex remodeling [PMID:27325793]
GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:10911987|PMID:17274630]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:15610740|PMID:16135807]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10911987]
EXISTENCE:direct assay:GO:0032777 Piccolo NuA4 histone acetyltransferase complex [PMID:12782659]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:22539722]
Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb; coding sequence contains length polymorphisms in different strains
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:18959479]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19387492|PMID:14562095]
EXISTENCE:genetic interaction:GO:0001731 formation of translation preinitiation complex [PMID:19387492]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:19387492]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:32503921]
EXISTENCE:mutant phenotype:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:32503921]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:32503921]
EXISTENCE:mutant phenotype:GO:1990113 RNA polymerase I assembly [PMID:23459708]
EXISTENCE:mutant phenotype:GO:1990114 RNA polymerase II core complex assembly [PMID:23459708]
EXISTENCE:mutant phenotype:GO:1990115 RNA polymerase III assembly [PMID:23459708]
EXISTENCE:mutant phenotype:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:32503921]
Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; mutations affect TOR signaling; ortholog of human URI/RMP
phenylalanine--tRNA ligase subunit alpha
EXISTENCE:direct assay:GO:0004826 phenylalanine-tRNA ligase activity [PMID:16162501|PMID:166841]
EXISTENCE:direct assay:GO:0006432 phenylalanyl-tRNA aminoacylation [PMID:16162501|PMID:166841]
EXISTENCE:direct assay:GO:0009328 phenylalanine-tRNA ligase complex [PMID:3049607]
EXISTENCE:mutant phenotype:GO:0002161 aminoacyl-tRNA editing activity [PMID:27226603]
Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10799523]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10799523]
EXISTENCE:genetic interaction:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:7568152]
EXISTENCE:genetic interaction:GO:0043565 sequence-specific DNA binding [PMID:7568152]
EXISTENCE:genetic interaction:GO:0090294 nitrogen catabolite activation of transcription [PMID:7568152|PMID:8622686]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:7568152]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9106207]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0090294 nitrogen catabolite activation of transcription [PMID:7568152|PMID:8622686]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:19380492]
Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18312373]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18312373]
Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme
hypothetical protein; conserved across S. cerevisiae strains; YFL019C is not an essential gene
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons
EXISTENCE:direct assay:GO:0004148 dihydrolipoyl dehydrogenase activity [PMID:2821168]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:12756305]
EXISTENCE:direct assay:GO:0009353 mitochondrial oxoglutarate dehydrogenase complex [PMID:2072900]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431|PMID:11926067]
EXISTENCE:genetic interaction:GO:0042743 hydrogen peroxide metabolic process [PMID:17110466]
EXISTENCE:mutant phenotype:GO:0004375 glycine dehydrogenase (decarboxylating) activity [PMID:7498764]
EXISTENCE:mutant phenotype:GO:0004591 oxoglutarate dehydrogenase (succinyl-transferring) activity [PMID:3528755]
EXISTENCE:mutant phenotype:GO:0004738 pyruvate dehydrogenase activity [PMID:3528755]
EXISTENCE:mutant phenotype:GO:0005960 glycine cleavage complex [PMID:7498764]
EXISTENCE:mutant phenotype:GO:0006090 pyruvate metabolic process [PMID:3528755]
EXISTENCE:mutant phenotype:GO:0006103 2-oxoglutarate metabolic process [PMID:3528755]
EXISTENCE:mutant phenotype:GO:0006546 glycine catabolic process [PMID:7498764]
EXISTENCE:mutant phenotype:GO:0006550 isoleucine catabolic process [PMID:1479341]
EXISTENCE:mutant phenotype:GO:0006552 leucine catabolic process [PMID:1479341]
EXISTENCE:mutant phenotype:GO:0006564 L-serine biosynthetic process [PMID:7498764]
EXISTENCE:mutant phenotype:GO:0006574 valine catabolic process [PMID:1479341]
EXISTENCE:mutant phenotype:GO:0042743 hydrogen peroxide metabolic process [PMID:17110466]
Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9012791|PMID:9630245]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
glucosamine 6-phosphate N-acetyltransferase
EXISTENCE:direct assay:GO:0004343 glucosamine 6-phosphate N-acetyltransferase activity [PMID:9867860]
EXISTENCE:direct assay:GO:0006048 UDP-N-acetylglucosamine biosynthetic process [PMID:9867860]
EXISTENCE:mutant phenotype:GO:0006048 UDP-N-acetylglucosamine biosynthetic process [PMID:9867860]
Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
ARS603.1; Very weak autonomously replicating sequence
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:9973563]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:15383543]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:9973563]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:10567545]
EXISTENCE:mutant phenotype:GO:0006458 'de novo' protein folding [PMID:8168133]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:7957078]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:8603724]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:8168133]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:8943361]
Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
hypothetical protein; conserved across S. cerevisiae strains; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20797624]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:20797624]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:20797624|PMID:10675505|PMID:16622836]
EXISTENCE:direct assay:GO:0007155 cell adhesion [PMID:11816034]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20797624]
EXISTENCE:mutant phenotype:GO:0007009 plasma membrane organization [PMID:20797624]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:20797624]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:20797624]
EXISTENCE:mutant phenotype:GO:0071470 cellular response to osmotic stress [PMID:20797624]
Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:12887900]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia
hypothetical protein; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005354 galactose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Hexose transporter; expressed at low levels and expression is repressed by glucose
EXISTENCE:mutant phenotype:GO:0006865 amino acid transport [PMID:8497191]
Protein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12062425|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
WW domain containing hypothetical protein; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
SCF ubiquitin ligase complex subunit CDC4
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:9346239]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0016363 nuclear matrix [PMID:2244914]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:23645675]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:direct assay:GO:0043224 nuclear SCF ubiquitin ligase complex [PMID:11080155]
EXISTENCE:direct assay:GO:0050815 phosphoserine residue binding [PMID:23314252]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:9346238]
EXISTENCE:genetic interaction:GO:0000086 G2/M transition of mitotic cell cycle [PMID:7954792]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:10409741]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:10409741]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:328339]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:21098119]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238|PMID:9346239]
F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p
ARS603.5; Autonomously replicating sequence on Chromosome VI; activated early in S phase in 50% of cell cycles
EXISTENCE:direct assay:GO:0000217 DNA secondary structure binding [PMID:9727028]
EXISTENCE:direct assay:GO:0003680 minor groove of adenine-thymine-rich DNA binding [PMID:9727028]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:9727028]
EXISTENCE:direct assay:GO:0030892 mitotic cohesin complex [PMID:9990856]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:9335333]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:8276886]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11448778|PMID:15280507]
Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12973301|PMID:15778719|PMID:22908043]
EXISTENCE:direct assay:GO:0016504 peptidase activator activity [PMID:22025621]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:23982732]
EXISTENCE:direct assay:GO:0061136 regulation of proteasomal protein catabolic process [PMID:22025621]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:22025621]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:17911101]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:12973301]
EXISTENCE:mutant phenotype:GO:0070628 proteasome binding [PMID:22025621]
EXISTENCE:mutant phenotype:GO:1902906 proteasome storage granule assembly [PMID:23982732]
EXISTENCE:mutant phenotype:GO:1990236 proteasome core complex import into nucleus [PMID:23982732]
Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
ARS604; Extremely inefficient ARS on Chromosome VI
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9128251]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:2111819]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:2111819]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0006887 exocytosis [PMID:15772160]
EXISTENCE:direct assay:GO:0030133 transport vesicle [PMID:23079598]
EXISTENCE:direct assay:GO:0031982 vesicle [PMID:12456647]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:22832273]
EXISTENCE:genetic interaction:GO:0006914 autophagy [PMID:20444978]
EXISTENCE:genetic interaction:GO:0007107 membrane addition at site of cytokinesis [PMID:12456647]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:9017592]
EXISTENCE:mutant phenotype:GO:0006906 vesicle fusion [PMID:9017592]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:20444978]
EXISTENCE:mutant phenotype:GO:0007107 membrane addition at site of cytokinesis [PMID:12456647]
EXISTENCE:mutant phenotype:GO:0031321 ascospore-type prospore assembly [PMID:9425151]
Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane; exocyst association is negatively regulated via phosphorylation by Cdc5p during cytokinesis
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11823419|PMID:26928762]
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:17079729]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17079729]
EXISTENCE:direct assay:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:direct assay:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:genetic interaction:GO:0006797 polyphosphate metabolic process [PMID:11102525]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:11102525]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:10480897]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:11823419]
EXISTENCE:physical interaction:GO:0033254 vacuolar transporter chaperone complex [PMID:11823419]
Regulatory subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; targeted to vacuole via AP-3 pathway; VTC2 has a paralog, VTC3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11454751]
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0062037 D-loop DNA binding [PMID:30467025]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:11454751|PMID:18615017|PMID:7622037]
EXISTENCE:physical interaction:GO:0062128 MutSgamma complex [PMID:9374523]
Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
ARS605; Autonomously replicating sequence on Chromosome VI; activated early in S phase in 27% of cell cycles; inactive during premeiotic S phase
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
YFLWTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
putative ATP-dependent RNA helicase SPB4
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:9769101]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9769101|PMID:14562095]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:21825077]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:22735702]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:21825077|PMID:2408148]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:2408148]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
EXISTENCE:physical interaction:GO:0030686 90S preribosome [PMID:21825077]
Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9430663]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9430663]
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:9430663]
EXISTENCE:direct assay:GO:0031119 tRNA pseudouridine synthesis [PMID:9430663]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:9430663]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:9430663]
EXISTENCE:mutant phenotype:GO:1990481 mRNA pseudouridine synthesis [PMID:25192136]
tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11309412]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16871394]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:31323064]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:27458021|PMID:17443350|PMID:23212245]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:16871394]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16871394]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16871394]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16871394]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:11309412]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614|PMID:27458021|PMID:31779129]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:31779129]
EXISTENCE:mutant phenotype:GO:0101031 chaperone complex [PMID:31779129]
EXISTENCE:physical interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
Nucleolar protein involved in the asymmetric localization of ASH1 mRNA; binds cooperatively with She2p to the E3 zip-code element in the 3'-UTR of the mRNA; constituent of 66S pre-ribosomal particles involved in pre-rRNA processing and nuclear export of the 60S subunit; may facilitate Rpl43p loading; required post-transcriptionally for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:7688296]
EXISTENCE:direct assay:GO:0044612 nuclear pore linkers [PMID:18046406]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:9813081]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11071906]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220|PMID:7828598]
EXISTENCE:mutant phenotype:GO:0006999 nuclear pore organization [PMID:11121302|PMID:8682854]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:18206968]
Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
type 1 protein phosphatase-activating protein YPI1
EXISTENCE:direct assay:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:14506263]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17142459|PMID:18172024]
EXISTENCE:direct assay:GO:0032515 negative regulation of phosphoprotein phosphatase activity [PMID:14506263]
EXISTENCE:direct assay:GO:0035308 negative regulation of protein dephosphorylation [PMID:14506263]
EXISTENCE:direct assay:GO:1900180 regulation of protein localization to nucleus [PMID:18172024]
EXISTENCE:genetic interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:18172024]
EXISTENCE:genetic interaction:GO:0032516 positive regulation of phosphoprotein phosphatase activity [PMID:18172024]
EXISTENCE:genetic interaction:GO:0072542 protein phosphatase activator activity [PMID:18172024]
EXISTENCE:mutant phenotype:GO:0004865 protein serine/threonine phosphatase inhibitor activity [PMID:14506263]
EXISTENCE:mutant phenotype:GO:0005977 glycogen metabolic process [PMID:14506263]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:22367039]
EXISTENCE:mutant phenotype:GO:0032515 negative regulation of phosphoprotein phosphatase activity [PMID:14506263]
EXISTENCE:mutant phenotype:GO:0035308 negative regulation of protein dephosphorylation [PMID:14506263]
EXISTENCE:mutant phenotype:GO:1900180 regulation of protein localization to nucleus [PMID:17142459]
Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
proteasome regulatory particle lid subunit RPN11
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15210724]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19773362]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19773362]
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:25389291]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:direct assay:GO:0140492 metal-dependent deubiquitinase activity [PMID:25389291]
EXISTENCE:genetic interaction:GO:0016579 protein deubiquitination [PMID:14581483]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:20941496]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:19773362]
EXISTENCE:mutant phenotype:GO:0016559 peroxisome fission [PMID:19773362]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:12183636]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12183636]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:20061387]
EXISTENCE:mutant phenotype:GO:0140492 metal-dependent deubiquitinase activity [PMID:12353037|PMID:12183636]
EXISTENCE:mutant phenotype:GO:1902906 proteasome storage granule assembly [PMID:23936414]
Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:24681967]
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:10022888]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10022888]
Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle
Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18004758]
Putative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23625923]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:9234705]
EXISTENCE:mutant phenotype:GO:0006448 regulation of translational elongation [PMID:9234705]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:32320318]
Positive regulator of the Gcn2p kinase activity; important for disaggregation of ordered and disordered protein aggregates; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; suppresses frameshifting at stalled ribosomes
EXISTENCE:direct assay:GO:0000502 proteasome complex [PMID:12408819]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:12408819|PMID:17018280]
EXISTENCE:direct assay:GO:0005838 proteasome regulatory particle [PMID:11029046]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:12408819|PMID:17018280]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:12408819]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:21070972]
EXISTENCE:mutant phenotype:GO:1901799 negative regulation of proteasomal protein catabolic process [PMID:17018280]
Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains en bloc, rather than from the distal tip of the chain; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance; human homolog UBP14 complements yeast null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:21944719|PMID:21987634|PMID:22009199]
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane and may connect Mic60p with the Mic10p-Mic12p-Mic27p subcomplex; both yeast and human Mic19p become oxidized, and oxidation may regulate MICOS
Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
ARS606; Highly-efficient autonomously replicating sequence on Chromosome VI; activated early in S phase in 74% of cell cycles; replication capacity dependent upon Sum1p
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:33002606]
hypothetical protein; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication
hypothetical protein; identified by homology
EXISTENCE:curator inference:GO:0006338 chromatin remodeling [PMID:12482963]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12482963]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12482963]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:12482963]
EXISTENCE:direct assay:GO:0036436 Isw1a complex [PMID:12482963]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:27185881]
Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
calmodulin-dependent protein kinase CMK1
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004683 calmodulin-dependent protein kinase activity [PMID:2061341]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9756868]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:11743609]
Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004373 glycogen (starch) synthase activity [PMID:2123485]
EXISTENCE:mutant phenotype:GO:0005978 glycogen biosynthetic process [PMID:1908457]
Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:31699995]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:31699995]
EXISTENCE:direct assay:GO:0032233 positive regulation of actin filament bundle assembly [PMID:31699995]
Protein that positively regulates actin assembly; forms a homodimer and interacts with G-actin; localization to the bud neck and bud tip requires Spa2p; YFR016C is not an essential gene
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:21585652]
EXISTENCE:mutant phenotype:GO:0004857 enzyme inhibitor activity [PMID:21585652]
EXISTENCE:mutant phenotype:GO:0045818 negative regulation of glycogen catabolic process [PMID:21585652]
Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
hypothetical protein; SWAT-GFP and seamless GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole
1-phosphatidylinositol-3-phosphate 5-kinase
EXISTENCE:direct assay:GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity [PMID:9811604]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11889142|PMID:9763421]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:11889142|PMID:9763421]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:9702203]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:9811604]
EXISTENCE:direct assay:GO:0070772 PAS complex [PMID:19037259]
EXISTENCE:mutant phenotype:GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity [PMID:9763421|PMID:9811604]
EXISTENCE:mutant phenotype:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:9763421|PMID:9811604]
EXISTENCE:physical interaction:GO:0070772 PAS complex [PMID:19037259]
1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:26928762]
hypothetical protein, secreted when constitutively expressed; SWAT-GFP fusion protein localizes to the endoplasmic reticulum (ER) and extracellular region, while mCherry fusion protein localizes to the ER and vacuole; mRNA identified as translated by ribosome profiling data; CSS2 is a non-essential gene
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15103325|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:18497569]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:18769150]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11707261]
EXISTENCE:direct assay:GO:0006624 vacuolar protein processing [PMID:11707261]
EXISTENCE:direct assay:GO:0016236 macroautophagy [PMID:11707261]
EXISTENCE:direct assay:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:11707261]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:15103325|PMID:15155809]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:15155809]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:15103325]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:11707261]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11707261]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:11739783]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:11707261|PMID:16876790]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044090 positive regulation of vacuole organization [PMID:17699591]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:15103325]
Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (CVT) pathway; binds PtdIns(3,5)P2, PI3P and PI4P; WD-40 repeat containing protein and PROPPIN family member; relocalizes from the vacuole to cytoplasm upon DNA replication stress; mammalian homologs include: WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood (SENDA)
EXISTENCE:direct assay:GO:0031625 ubiquitin protein ligase binding [PMID:24820415]
EXISTENCE:genetic interaction:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:24820415]
EXISTENCE:genetic interaction:GO:0002092 positive regulation of receptor internalization [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:24820415]
EXISTENCE:mutant phenotype:GO:0031625 ubiquitin protein ligase binding [PMID:24820415]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
Alpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; SWAT-GFP and mCherry fusion proteins localize to the cytosol; ROG3 has a paralog, ROD1, that arose from the whole genome duplication
RNA of Unknown Function; precise location of 3' end uncertain; RUF22 may be longer than currently annotated
ARS607; Highly-efficient autonomously replicating sequence on Chromosome VI; activated very early in S phase in >85% of cell cycles
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:28193845]
EXISTENCE:genetic interaction:GO:0006417 regulation of translation [PMID:28193845]
EXISTENCE:genetic interaction:GO:0010609 mRNA localization resulting in post-transcriptional regulation of gene expression [PMID:28193845]
EXISTENCE:genetic interaction:GO:0016071 mRNA metabolic process [PMID:28193845]
Putative RNA binding protein; partially redundant function with paralog MIP6 in the steady-state expression, translational timing and localization of a subset of Ndt80-dependent mRNAs that are protected until translated near the end of meiosis II; regulator of meiotic commitment; redundant function with MIP6 in sporulation; localizes to puncta near the openings of the prospore membrane; suppressor of a DNA polymerase epsilon mutation; induced by NDT80
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:19158382]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:19158382]
Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241]
EXISTENCE:mutant phenotype:GO:0004401 histidinol-phosphatase activity [PMID:14190241]
Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:19073890]
hypothetical protein; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR); SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:11448778]
EXISTENCE:direct assay:GO:0016407 acetyltransferase activity [PMID:11864574|PMID:18614053|PMID:22593213]
EXISTENCE:direct assay:GO:0018393 internal peptidyl-lysine acetylation [PMID:18614053]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16962805]
EXISTENCE:genetic interaction:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:16934511]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:9990856]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11448778]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:34897432]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0016407 acetyltransferase activity [PMID:22593213]
EXISTENCE:mutant phenotype:GO:0032200 telomere organization [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:9990855|PMID:9990856]
EXISTENCE:mutant phenotype:GO:0034089 establishment of meiotic sister chromatid cohesion [PMID:35103590]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0140588 chromatin looping [PMID:32032532]
Acetyltransferase required for sister chromatid cohesion; elicits cohesin dimerization during S phase; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by Cdc28p, Cdc7p, Mck1p to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; relative distribution to nucleus increases upon DNA replication stress; mutations in human homolog ESCO2 cause Roberts syndrome
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:9295359|PMID:17173039|PMID:24319056|PMID:24954044]
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:26085509|PMID:35238874]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26090959|PMID:11914276]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12062061|PMID:26090959|PMID:36259662]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26090959]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:12062061]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:36259662]
EXISTENCE:direct assay:GO:0010508 positive regulation of autophagy [PMID:35238874]
EXISTENCE:direct assay:GO:0030869 RENT complex [PMID:10219244]
EXISTENCE:direct assay:GO:0044732 mitotic spindle pole body [PMID:36259662]
EXISTENCE:direct assay:GO:0071958 new mitotic spindle pole body [PMID:36259662]
EXISTENCE:direct assay:GO:0140013 meiotic nuclear division [PMID:35238874]
EXISTENCE:genetic interaction:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:26085509]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:6749599]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:29694832]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16769819]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:10219244]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19158678]
EXISTENCE:mutant phenotype:GO:0032467 positive regulation of cytokinesis [PMID:25371407]
EXISTENCE:mutant phenotype:GO:0051229 meiotic spindle disassembly [PMID:12737806]
EXISTENCE:mutant phenotype:GO:0071470 cellular response to osmotic stress [PMID:25411400]
EXISTENCE:mutant phenotype:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:26085509]
EXISTENCE:mutant phenotype:GO:2000786 positive regulation of autophagosome assembly [PMID:29694832]
Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response, autophagy during meiosis; downregulates microautophagy induction after TORC1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10409731]
EXISTENCE:mutant phenotype:GO:0043200 response to amino acid [PMID:10409731]
Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
EXISTENCE:direct assay:GO:0004783 sulfite reductase (NADPH) activity [PMID:6751400]
EXISTENCE:direct assay:GO:0009337 sulfite reductase complex (NADPH) [PMID:6751400]
EXISTENCE:mutant phenotype:GO:0000103 sulfate assimilation [PMID:19236486|PMID:7928966]
Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide
ARS608; Extremely inefficient autonomously replicating sequence on Chr VI; activated early in S phase in <10% of cell cycles
EXISTENCE:curator inference:GO:0007130 synaptonemal complex assembly [PMID:10811823]
EXISTENCE:curator inference:GO:0010032 meiotic chromosome condensation [PMID:10811823]
EXISTENCE:curator inference:GO:0051307 meiotic chromosome separation [PMID:10811823]
EXISTENCE:direct assay:GO:0000796 condensin complex [PMID:10811823]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16055730]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:9727028]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12719426]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:10811823]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:10811823]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:18708579]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:10811823]
EXISTENCE:mutant phenotype:GO:1903342 negative regulation of meiotic DNA double-strand break formation [PMID:25103240]
Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:18263608]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:18263608]
Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
RNA of Unknown Function; precise location of 3' end uncertain; RUF23 may be longer than currently annotated
hypothetical protein; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
Mitochondrial hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
ubiquinol--cytochrome-c reductase subunit 6
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11914276|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:8288589]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:8288589]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:8288589]
Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
phosphate-sensing transcription factor PHO4
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18408055]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10320381]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10320381]
EXISTENCE:direct assay:GO:0016036 cellular response to phosphate starvation [PMID:10320381]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:2183025|PMID:2664469|PMID:19111667|PMID:19158363]
EXISTENCE:genetic interaction:GO:0045937 positive regulation of phosphate metabolic process [PMID:3915785]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3915785]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:2183025]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:2196175]
EXISTENCE:mutant phenotype:GO:0016036 cellular response to phosphate starvation [PMID:10320381]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3139496|PMID:4616940]
Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability
hypothetical protein; deletion mutant exhibits synthetic phenotype with alpha-synuclein
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
anaphase promoting complex subunit CDC26
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:8895471]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:12399376]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:8895471]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:8895471]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16461455]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:15870268]
Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:18085829]
EXISTENCE:mutant phenotype:GO:0070987 error-free translesion synthesis [PMID:30111537]
Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci; SWAT-GFP and mCherry fusion proteins localize to the nucleus
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly
EXISTENCE:direct assay:GO:1903765 negative regulation of potassium ion export across plasma membrane [PMID:15947196]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8649382]
EXISTENCE:mutant phenotype:GO:1903765 negative regulation of potassium ion export across plasma membrane [PMID:15947196]
Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17157937|PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:17157937]
Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17893149]
EXISTENCE:mutant phenotype:GO:0006078 (1->6)-beta-D-glucan biosynthetic process [PMID:17893149]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18439903]
Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:22993212]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:22993212]
EXISTENCE:physical interaction:GO:0016192 vesicle-mediated transport [PMID:22993212]
Clathrin coat accessory factor; involved in clathrin-mediated vesicle trafficking; may function to link the AP-1 clathrin adaptor complex with the Rab GTPase Ypt31p; has structural similarity to G-proteins; mouse homolog Aagab (p34) functionally complements irc6 null mutation; null mutant displays increased levels of spontaneous Rad52p foci
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17179087|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0070573 metallodipeptidase activity [PMID:19346245]
EXISTENCE:genetic interaction:GO:0008242 omega peptidase activity [PMID:17179087]
EXISTENCE:mutant phenotype:GO:0006751 glutathione catabolic process [PMID:17179087]
EXISTENCE:mutant phenotype:GO:0070573 metallodipeptidase activity [PMID:19346245]
Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant
Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14633972]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:22561345|PMID:22561346]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:22561345|PMID:22561346]
EXISTENCE:mutant phenotype:GO:1905560 negative regulation of kinetochore assembly [PMID:22561345]
EXISTENCE:physical interaction:GO:0007059 chromosome segregation [PMID:14633972|PMID:9520439]
Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
nicotinate-nucleotide diphosphorylase (carboxylating)
EXISTENCE:genetic interaction:GO:0034354 'de novo' NAD biosynthetic process from tryptophan [PMID:12062417]
EXISTENCE:mutant phenotype:GO:0004514 nicotinate-nucleotide diphosphorylase (carboxylating) activity [PMID:12062417]
Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
Cytosolic protein required for sporulation
mitochondrial 37S ribosomal protein YMR31
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2693936]
EXISTENCE:direct assay:GO:0004591 oxoglutarate dehydrogenase (succinyl-transferring) activity [PMID:25165143]
EXISTENCE:direct assay:GO:0005761 mitochondrial ribosome [PMID:2693936]
EXISTENCE:direct assay:GO:0009353 mitochondrial oxoglutarate dehydrogenase complex [PMID:25165143]
EXISTENCE:mutant phenotype:GO:0004591 oxoglutarate dehydrogenase (succinyl-transferring) activity [PMID:25165143]
EXISTENCE:mutant phenotype:GO:0006099 tricarboxylic acid cycle [PMID:25165143]
EXISTENCE:mutant phenotype:GO:0006103 2-oxoglutarate metabolic process [PMID:25165143]
Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; exists in two forms derived from a single mRNA translated from two alternative start sites; has similarity to human mitochondrial ribosomal protein MRP-S36
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:9087403]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:21435344]
EXISTENCE:genetic interaction:GO:0048313 Golgi inheritance [PMID:18595704]
EXISTENCE:genetic interaction:GO:0090167 Golgi distribution to daughter cells [PMID:18595704]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8617224]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8617224]
EXISTENCE:mutant phenotype:GO:0030126 COPI vesicle coat [PMID:8617224]
Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
proteasome regulatory particle lid subunit RPN12
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:9741626]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10490625]
Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004396 hexokinase activity [PMID:12611889|PMID:332086]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11254124]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:14562095]
EXISTENCE:direct assay:GO:0006013 mannose metabolic process [PMID:332086]
EXISTENCE:direct assay:GO:0006096 glycolytic process [PMID:332086]
EXISTENCE:genetic interaction:GO:0046323 glucose import [PMID:6300872]
EXISTENCE:genetic interaction:GO:1990539 fructose import across plasma membrane [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0004396 hexokinase activity [PMID:6341351]
EXISTENCE:mutant phenotype:GO:0006000 fructose metabolic process [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0006006 glucose metabolic process [PMID:6300872]
Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
ARS609; Autonomously replicating sequence on Chromosome VI; activated very late in S phase in 37% of cell cycles; this origin is specifically fired in the absence of RAD53 in the presence of hydroxyurea
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:direct assay:GO:0006790 sulfur compound metabolic process [PMID:21569935]
EXISTENCE:direct assay:GO:0019450 L-cysteine catabolic process to pyruvate [PMID:21569935]
EXISTENCE:direct assay:GO:0047804 cysteine-S-conjugate beta-lyase activity [PMID:21569935]
EXISTENCE:mutant phenotype:GO:0006790 sulfur compound metabolic process [PMID:21569935]
EXISTENCE:mutant phenotype:GO:0019450 L-cysteine catabolic process to pyruvate [PMID:21569935]
EXISTENCE:mutant phenotype:GO:0047804 cysteine-S-conjugate beta-lyase activity [PMID:21569935]
Cysteine desulphydrase, enables growth on cysteine as nitrogen source; involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner
Telomere-linked gene, silencing of which is strictly Sir2-dependent
ARS610; Autonomously Replicating Sequence
TEL06R; Telomeric region on the right arm of Chromosome VI; composed of an X element core sequence and a short terminal stretch of telomeric repeats