Saccharomyces cerevisiae S288C chromosome V, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome V
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (29-JUL-2014) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006939.\n\nOn Apr 26, 2011 this sequence version replaced NC_001137.2.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL05L; Telomeric region on the left arm of Chromosome V; annotated components include an X element core sequence and a long Y' element; TEL05L does have telomeric repeats (TEL05L-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
Helicase-like protein encoded within the telomeric Y' element
ARS502; Autonomously Replicating Sequence
ARS503; Autonomously Replicating Sequence
hypothetical protein; located adjacent to ARS503 and the telomere on the left arm of chromosome V; regulated by inositol/choline
ARS504; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
Protein required for sporulation; localizes to the peroxisome
EXISTENCE:direct assay:GO:0004458 D-lactate dehydrogenase (cytochrome) activity [PMID:10509019]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10509019|PMID:11914276]
EXISTENCE:direct assay:GO:0071949 FAD binding [PMID:26774271]
EXISTENCE:direct assay:GO:0099615 (D)-2-hydroxyglutarate-pyruvate transhydrogenase activity [PMID:26774271]
EXISTENCE:mutant phenotype:GO:0019249 lactate biosynthetic process [PMID:26774271]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:16999827]
EXISTENCE:mutant phenotype:GO:0099615 (D)-2-hydroxyglutarate-pyruvate transhydrogenase activity [PMID:26774271]
2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase; converts D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate in the presence of FAD, with concomitant reduction of pyruvate to D-lactate; minor lactate dehydrogenase activity; component of the retrograde regulon that consists of genes whose expression are stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source; located in the cytoplasm
EXISTENCE:mutant phenotype:GO:0046029 mannitol dehydrogenase activity [PMID:26996892]
Mannitol dehydrogenase; deletion suppresses mutation of mpt5; DSF1 has a paralog, MAN2, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:genetic interaction:GO:0015795 sorbitol transmembrane transport [PMID:26996892]
EXISTENCE:genetic interaction:GO:0015797 mannitol transmembrane transport [PMID:26996892]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Putative transmembrane polyol transporter; supports growth on and uptake of mannitol and sorbitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; induced by non-fermentable carbon sources; induced in low glucose, repressed in high glucose; HXT13 has a paralog, HXT17, that arose from a segmental duplication
hypothetical protein; expressed at both mRNA and protein levels; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0006473 protein acetylation [PMID:23775086]
EXISTENCE:direct assay:GO:0008080 N-acetyltransferase activity [PMID:23775086]
EXISTENCE:direct assay:GO:0047812 D-amino-acid N-acetyltransferase activity [PMID:15375647]
EXISTENCE:mutant phenotype:GO:0046416 D-amino acid metabolic process [PMID:16362288]
EXISTENCE:mutant phenotype:GO:0070458 cellular detoxification of nitrogen compound [PMID:16362288|PMID:23775086]
D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:19469713]
EXISTENCE:direct assay:GO:0006879 cellular iron ion homeostasis [PMID:9884238]
EXISTENCE:direct assay:GO:0031410 cytoplasmic vesicle [PMID:10748025]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9884238]
EXISTENCE:mutant phenotype:GO:0015344 siderophore uptake transmembrane transporter activity [PMID:9884238]
EXISTENCE:mutant phenotype:GO:0015891 siderophore transport [PMID:9884238]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9884238]
Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11274162]
Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15536122]
EXISTENCE:direct assay:GO:0015174 basic amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0015802 basic amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:29402931|PMID:15536122]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10654085]
EXISTENCE:genetic interaction:GO:1903826 L-arginine transmembrane transport [PMID:23047103]
EXISTENCE:mutant phenotype:GO:0061459 L-arginine transmembrane transporter activity [PMID:10753927]
Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
nitrogen permease regulating protein NPR2
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:direct assay:GO:1990130 GATOR1 complex [PMID:21900499]
EXISTENCE:genetic interaction:GO:0010508 positive regulation of autophagy [PMID:25046117]
EXISTENCE:genetic interaction:GO:1904262 negative regulation of TORC1 signaling [PMID:23716719|PMID:25046117]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:12869630]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:25046117]
EXISTENCE:mutant phenotype:GO:0015824 proline transport [PMID:7867803]
EXISTENCE:mutant phenotype:GO:0015840 urea transport [PMID:7867803]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:19521502|PMID:23716719|PMID:25046117]
EXISTENCE:mutant phenotype:GO:2000785 regulation of autophagosome assembly [PMID:21900499]
Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11511347]
EXISTENCE:direct assay:GO:0005828 kinetochore microtubule [PMID:19041752]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11511347|PMID:1618897]
EXISTENCE:direct assay:GO:0008569 minus-end-directed microtubule motor activity [PMID:21350123]
EXISTENCE:direct assay:GO:0008574 plus-end-directed microtubule motor activity [PMID:21350123]
EXISTENCE:direct assay:GO:0033047 regulation of mitotic sister chromatid segregation [PMID:31253793]
EXISTENCE:genetic interaction:GO:0000070 mitotic sister chromatid segregation [PMID:7860634]
EXISTENCE:genetic interaction:GO:0000073 initial mitotic spindle pole body separation [PMID:1618897|PMID:1618910]
EXISTENCE:genetic interaction:GO:0003777 microtubule motor activity [PMID:1618910]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:23851487|PMID:9813090]
EXISTENCE:mutant phenotype:GO:0000073 initial mitotic spindle pole body separation [PMID:1618897]
EXISTENCE:mutant phenotype:GO:0000235 astral microtubule [PMID:19041752]
EXISTENCE:mutant phenotype:GO:0007019 microtubule depolymerization [PMID:19041752]
EXISTENCE:mutant phenotype:GO:0008569 minus-end-directed microtubule motor activity [PMID:27216310]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:31964755]
EXISTENCE:mutant phenotype:GO:0090307 mitotic spindle assembly [PMID:23851487|PMID:9813090]
Bipolar kinesin motor protein; switches from minus- to plus-end-directed motility in vitro depending on conditions; involved in mitotic spindle assembly and chromosome segregation
EXISTENCE:mutant phenotype:GO:0000425 pexophagy [PMID:31332264]
EXISTENCE:mutant phenotype:GO:0004252 serine-type endopeptidase activity [PMID:377296|PMID:385314]
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:2674123|PMID:385314]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:2674123]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:2674123]
Vacuolar proteinase B yscB with H3 N-terminal endopeptidase activity; serine protease of subtilisin family; involved in protein degradation in vacuole; required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via AP-3 pathway; PRB1 has a paralog, YSP3, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0004175 endopeptidase activity [PMID:10821182]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9604886]
EXISTENCE:mutant phenotype:GO:0033108 mitochondrial respiratory chain complex assembly [PMID:19703468]
EXISTENCE:physical interaction:GO:0042720 mitochondrial inner membrane peptidase complex [PMID:10821182]
Subunit of the mitochondrial inner membrane peptidase (IMP); IMP is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
hypothetical protein; null mutant is sensitive to heat, pH disturbances, ionic stress, and various chemicals; hypersensitivity to hygromycin B indicative of defects in vacuolar trafficking
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0004610 phosphoacetylglucosamine mutase activity [PMID:8174553]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:8174553]
Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
hypothetical protein; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; may have a role in telomere maintenance; target of UME6 regulation
EXISTENCE:direct assay:GO:0000123 histone acetyltransferase complex [PMID:8858151|PMID:9575221]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:8858151]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14761951|PMID:9575221]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8858151|PMID:9575221]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:8858151]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:8858151]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:10982821]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:8858151|PMID:9575221]
EXISTENCE:physical interaction:GO:0000123 histone acetyltransferase complex [PMID:8858151]
Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
EXISTENCE:direct assay:GO:0000027 ribosomal large subunit assembly [PMID:31745560]
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:12093911]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12093911|PMID:22842922]
EXISTENCE:direct assay:GO:0006364 rRNA processing [PMID:31745560]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:31745560|PMID:31413149]
EXISTENCE:direct assay:GO:0090070 positive regulation of ribosome biogenesis [PMID:31745560]
EXISTENCE:mutant phenotype:GO:0009303 rRNA transcription [PMID:12093911]
EXISTENCE:mutant phenotype:GO:0030685 nucleolar preribosome [PMID:31413149]
EXISTENCE:mutant phenotype:GO:0090070 positive regulation of ribosome biogenesis [PMID:31413149]
Protein involved in 60S assembly and pre-40S AFs recycling; has sequence similarity to human MybBP1A and weak sequence similarity to B-type DNA polymerases; not required for chromosomal DNA replication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U776 and small subunit (SSU) rRNA at position U759
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:9822666]
Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0031417 NatC complex [PMID:10504710]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:11274203]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:11274203]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functionally complement the null allele
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:17430883]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:17430883]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17430883]
Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain
H(+)-transporting V1 sector ATPase subunit D
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762|PMID:23836928]
EXISTENCE:direct assay:GO:0016471 vacuolar proton-transporting V-type ATPase complex [PMID:10801866]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:29519938]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:10801866]
Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
ARS507; Autonomously Replicating Sequence
mitochondrial 54S ribosomal protein RML2
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9445368]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:9445368]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:9079633]
Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:31804585]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at positions G2619 and U2724
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position A796
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:19220810]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:21453443]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:19220810]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:21453443]
Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9587404]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0016156 fumarate reductase (NADH) activity [PMID:8946166]
EXISTENCE:genetic interaction:GO:0071454 cellular response to anoxia [PMID:17345583|PMID:9711846]
Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004793 threonine aldolase activity [PMID:9163906]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12676688]
EXISTENCE:direct assay:GO:0006545 glycine biosynthetic process [PMID:3086094]
EXISTENCE:direct assay:GO:0006567 threonine catabolic process [PMID:3086094]
EXISTENCE:direct assay:GO:0008732 L-allo-threonine aldolase activity [PMID:9151955]
Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23207916]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
hypothetical protein; proposed role in Golgi vesicle trafficking based on phenotypic profile, presence of a coiled-coil domain often associated with vesicle tethering proteins, and interaction profile process enrichment; may interact with ribosomes, based on co-purification studies; localizes to the ER and cell periphery in HTP studies; contains fibronectin type III domain fold
EXISTENCE:direct assay:GO:0004382 GDP phosphatase activity [PMID:7506254]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:9614179]
EXISTENCE:direct assay:GO:0045134 UDP phosphatase activity [PMID:11425802]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:8391537]
Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies
EXISTENCE:direct assay:GO:0003951 NAD+ kinase activity [PMID:15978040]
EXISTENCE:direct assay:GO:0006741 NADP biosynthetic process [PMID:15978040]
EXISTENCE:direct assay:GO:0042736 NADH kinase activity [PMID:15978040]
ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:12045225]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953|PMID:15781460]
EXISTENCE:direct assay:GO:0016757 glycosyltransferase activity [PMID:23919454]
EXISTENCE:genetic interaction:GO:0006037 cell wall chitin metabolic process [PMID:18694928]
EXISTENCE:genetic interaction:GO:0016757 glycosyltransferase activity [PMID:18694928]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0006037 cell wall chitin metabolic process [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0016757 glycosyltransferase activity [PMID:18694928]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:18694928]
Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:8636074]
EXISTENCE:direct assay:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:7814361]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:7814361]
EXISTENCE:direct assay:GO:0009055 electron transfer activity [PMID:7814361]
Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0019509 L-methionine salvage from methylthioadenosine [PMID:18625006]
Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0000111 nucleotide-excision repair factor 2 complex [PMID:7768886]
EXISTENCE:direct assay:GO:0000224 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity [PMID:20016784]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:9813069|PMID:9837874]
EXISTENCE:direct assay:GO:0006517 protein deglycosylation [PMID:20016784]
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:11323716]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:25311859|PMID:15117949|PMID:15242647]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:26150418]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:15167887]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:19889839]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:15121879]
Proteasome-associated ubiquitin receptor; K48-specific Ub chain binding protein that recruits substrates to the proteasome; subunit of nuclear excision repair factor 2 (NER2) with Rad4p that binds damaged DNA during NER, linking repair to proteolysis; NER2 binds DDR gene promoters to repress transcription in the absence of damage; stimulates Png1p-induced protein deglycosylation; contains a Ub-like (UBL) and two Ub-associated (UBA) domains; UBA2 protects Rad23p from proteasomal degradation
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:10037752]
EXISTENCE:direct assay:GO:0000136 mannan polymerase complex [PMID:9430634]
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:9430634]
EXISTENCE:direct assay:GO:0006487 protein N-linked glycosylation [PMID:9430634]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:9430634]
Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
hypothetical protein; originally considered essential but deletion overlapped the TATA box of neighboring essential gene HYP2, while deletion that avoids the HYP2 TATA box is viable; transcription may be regulated by Gcr1p
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:19120453]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:21451136]
EXISTENCE:direct assay:GO:0003746 translation elongation factor activity [PMID:19424157]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:16215987]
EXISTENCE:direct assay:GO:0045901 positive regulation of translational elongation [PMID:19424157]
EXISTENCE:direct assay:GO:0045905 positive regulation of translational termination [PMID:19424157]
EXISTENCE:direct assay:GO:0045948 positive regulation of translational initiation [PMID:21451136]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:11861547]
EXISTENCE:genetic interaction:GO:0006452 translational frameshifting [PMID:19424157]
EXISTENCE:genetic interaction:GO:0045901 positive regulation of translational elongation [PMID:19338753|PMID:19424157]
EXISTENCE:mutant phenotype:GO:0003746 translation elongation factor activity [PMID:19424157]
EXISTENCE:mutant phenotype:GO:0045901 positive regulation of translational elongation [PMID:19338753|PMID:19424157]
EXISTENCE:mutant phenotype:GO:1903272 positive regulation of cytoplasmic translational elongation through polyproline stretches [PMID:24923804]
Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has paralog ANB1 that arose from whole genome duplication; in humans EIF5A variants cause a disorder characterized by variable combinations of developmental delay, microcephaly, micrognathia and dysmorphism; human EIF5A complements inviability of yeast hyp2 anb1 double null mutant
hypothetical protein; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:9554851]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10704410|PMID:8224843]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10704410|PMID:8224843|PMID:11914276]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12480933]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:18657510]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0042555 MCM complex [PMID:12480933]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:direct assay:GO:1904931 MCM complex binding [PMID:26912723]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:12060653]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10834843]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:12480933]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:19064704]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:19064704]
EXISTENCE:mutant phenotype:GO:1902975 mitotic DNA replication initiation [PMID:8224843]
EXISTENCE:physical interaction:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12058017|PMID:22745129|PMID:30785834]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:11791712]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:30785834]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:12058017]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:24392018]
EXISTENCE:mutant phenotype:GO:0055092 sterol homeostasis [PMID:22918956|PMID:30785834]
P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid and sterol composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1; null mutation is complemented by human Parkinson disease-related ATP13A1
ARS508; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11150530|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006626 protein targeting to mitochondrion [PMID:11150530]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:12943685]
Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:genetic interaction:GO:0042823 pyridoxal phosphate biosynthetic process [PMID:17696614]
EXISTENCE:mutant phenotype:GO:0042823 pyridoxal phosphate biosynthetic process [PMID:17696614]
Putative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; localizes to cytosol and peroxisomes; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
hypothetical protein; conserved among S. cerevisiae strains; YEL028W is not an essential gene
EXISTENCE:curator inference:GO:0006413 translational initiation [PMID:9023104]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0071074 eukaryotic initiation factor eIF2 binding [PMID:14698289]
Methionine initiator tRNA (tRNA-Met); predicted by tRNAscan-SE analysis; one of four initiator methionine tRNAs in yeast that are functional for translation
H(+)-transporting V0 sector ATPase subunit c
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15252052]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:2145283]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:9434348]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:9434348]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:2145283]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:2145283]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:2145283]
Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10684247]
EXISTENCE:direct assay:GO:0030621 U4 snRNA binding [PMID:17631273|PMID:14730029]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:11081632]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:17631273|PMID:14730029]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:1905216 positive regulation of RNA binding [PMID:23509373]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:14730029|PMID:11720284]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14730029]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:14730029]
RNA binding protein; part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:26928762]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
ubiquinol--cytochrome-c reductase catalytic subunit RIP1
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857|PMID:3036836]
EXISTENCE:direct assay:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:3036836]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:2537289]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:3036836]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
hypothetical protein; expression is increased greatly during sporulation; YEL023C is not an essential gene
Arf family guanine nucleotide exchange factor GEA2
EXISTENCE:direct assay:GO:0000137 Golgi cis cisterna [PMID:11294905]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10430582]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11294905]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:14668359]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11493664]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11493664]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20444982]
EXISTENCE:mutant phenotype:GO:0033363 secretory granule organization [PMID:14734650]
Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004590 orotidine-5'-phosphate decarboxylase activity [PMID:2061334]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18812321]
EXISTENCE:direct assay:GO:0006222 UMP biosynthetic process [PMID:2061334]
EXISTENCE:mutant phenotype:GO:0004590 orotidine-5'-phosphate decarboxylase activity [PMID:5651325|PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process [PMID:5651325|PMID:4550660]
EXISTENCE:mutant phenotype:GO:0006222 UMP biosynthetic process [PMID:4550660|PMID:5651325]
Orotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound
EXISTENCE:direct assay:GO:0042719 mitochondrial intermembrane space protein transporter complex [PMID:9889188|PMID:9822593]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:9495346]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:10469659|PMID:9822593]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:12138093]
EXISTENCE:direct assay:GO:0140318 protein transporter activity [PMID:9822593]
EXISTENCE:genetic interaction:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9822593]
EXISTENCE:genetic interaction:GO:0140318 protein transporter activity [PMID:9822593]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9889188]
Essential protein of the mitochondrial intermembrane space; forms a complex with Tim10p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:19095620|PMID:9620854]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:19095620|PMID:9620854]
EXISTENCE:direct assay:GO:0008033 tRNA processing [PMID:9620854]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:1990278]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:1990278]
EXISTENCE:mutant phenotype:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
RNA component of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; may be responsible for recognition of substrate tRNAs
putative indolepyruvate decarboxylase family protein
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27392156|PMID:26928762]
Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15738391]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391|PMID:31765407]
EXISTENCE:direct assay:GO:0030915 Smc5-Smc6 complex [PMID:15738391]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391|PMID:7788712]
EXISTENCE:mutant phenotype:GO:0019789 SUMO transferase activity [PMID:15738391|PMID:24108357|PMID:31765407]
EXISTENCE:mutant phenotype:GO:0030291 protein serine/threonine kinase inhibitor activity [PMID:24108357]
EXISTENCE:mutant phenotype:GO:1990683 DNA double-strand break attachment to nuclear envelope [PMID:27056668]
Highly conserved SUMO E3 ligase subunit of SMC5-SMC6 complex; required for anchoring dsDNA breaks to the nuclear periphery; SMC5-SMC6 plays a key role in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; required for efficient sister chromatid cohesion; mutants are sensitive to MMS, show increased spontaneous mutation and mitotic recombination; SUMOylates and inhibits Snf1p function; supports nucleolar function
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911]
Non-essential subunit of the NuA4 acetyltransferase complex; Esa1p-associated factor; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:8063750]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:8063750]
EXISTENCE:genetic interaction:GO:0032781 positive regulation of ATP-dependent activity [PMID:8063750]
Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
hypothetical protein may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery
nucleotide diphosphatase/phosphodiesterase NPP2
EXISTENCE:direct assay:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
EXISTENCE:genetic interaction:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:genetic interaction:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0009141 nucleoside triphosphate metabolic process [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; NPP2 has a paralog, NPP1, that arose from the whole genome duplication; npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to single mutants
EXISTENCE:direct assay:GO:0000932 P-body [PMID:15020463]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:24492965]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23706738|PMID:22842922]
EXISTENCE:genetic interaction:GO:0033962 P-body assembly [PMID:18981231]
EXISTENCE:mutant phenotype:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:15225544]
EXISTENCE:mutant phenotype:GO:0033962 P-body assembly [PMID:17984320|PMID:18981231]
EXISTENCE:mutant phenotype:GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:23956223|PMID:24492965]
Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16301533|PMID:9490720|PMID:9664035|PMID:9739084|PMID :26928762]
EXISTENCE:direct assay:GO:0000425 pexophagy [PMID:31262095]
EXISTENCE:direct assay:GO:0006914 autophagy [PMID:31262095]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10888680]
EXISTENCE:direct assay:GO:0034517 ribophagy [PMID:31262095]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:10888680]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:10888680]
EXISTENCE:direct assay:GO:0071563 Myo2p-Vac17p-Vac8p transport complex [PMID:12529432]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:16301533|PMID:8885233|PMID:10888680]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:31649143]
EXISTENCE:mutant phenotype:GO:0000407 phagophore assembly site [PMID:31649143]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:15901835]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:34893607]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:32508216|PMID:34893607]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:12529432|PMID:18701704]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:11441010|PMID:16301533]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:12594460]
EXISTENCE:mutant phenotype:GO:0051656 establishment of organelle localization [PMID:32508216]
EXISTENCE:mutant phenotype:GO:0071255 Cvt vesicle assembly [PMID:10837477]
EXISTENCE:mutant phenotype:GO:0071562 nucleus-vacuole junction assembly [PMID:10888680]
EXISTENCE:mutant phenotype:GO:1903044 protein localization to membrane raft [PMID:10888680]
EXISTENCE:physical interaction:GO:0043495 protein-membrane adaptor activity [PMID:12594460]
EXISTENCE:physical interaction:GO:0071561 nucleus-vacuole junction [PMID:10888680]
EXISTENCE:physical interaction:GO:0071563 Myo2p-Vac17p-Vac8p transport complex [PMID:12529432|PMID:12594460]
Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; mediates anchoring of phosphatidylinositol 3-kinase complex I (PIK3C3-C1) to autophagosome; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvj1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:1869573]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10811607]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10811607]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29519818]
Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0003844 1,4-alpha-glucan branching enzyme activity [PMID:1729600]
EXISTENCE:mutant phenotype:GO:0005978 glycogen biosynthetic process [PMID:1729600]
Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functionally complemented by human GBE1, which is associated with glycogen storage disease
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
hypothetical protein; conserved among S. cerevisiae strains; overlaps ORF YEL010W
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
amino acid starvation-responsive transcription factor GCN4
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:19940160]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18794364]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:16581788]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12455686|PMID:14648200]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:2204805|PMID:3290651|PMID:3464968|PMID:3530496|PMID: 3907851|PMID:19158363]
EXISTENCE:direct assay:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:18794364]
EXISTENCE:genetic interaction:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15121833]
EXISTENCE:genetic interaction:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:1425591]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15590823|PMID:26979516]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:21148207]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:16581788|PMID:19940160]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:16581788]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:31962153]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:21183953]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:15121833]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0060261 positive regulation of transcription initiation by RNA polymerase II [PMID:1425591]
EXISTENCE:mutant phenotype:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:mutant phenotype:GO:1990139 protein localization to nuclear periphery [PMID:31211995]
EXISTENCE:physical interaction:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:19940160]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:20308326|PMID:21183953]
bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels; contains four upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
One of four upstream open reading frames (uORFs) in 5' untranslated region of GCN4 gene, regulate translation
hypothetical protein; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism
Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:genetic interaction:GO:0035352 NAD transmembrane transport [PMID:16291748]
EXISTENCE:genetic interaction:GO:0051724 NAD transmembrane transporter activity [PMID:16291748]
EXISTENCE:mutant phenotype:GO:0035352 NAD transmembrane transport [PMID:16291748]
EXISTENCE:mutant phenotype:GO:0051724 NAD transmembrane transporter activity [PMID:16291748]
Mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication; human NAD+ transporter MCART1/SLC25A51 functionally complements the yia6 yea6 double null mutant, and yeast YEA6 reciprocally complements defects in MCART1/SLC25A51 null cells
ARS510; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10788474]
EXISTENCE:genetic interaction:GO:0015786 UDP-glucose transmembrane transport [PMID:18693752]
EXISTENCE:mutant phenotype:GO:0005462 UDP-N-acetylglucosamine transmembrane transporter activity [PMID:10788474]
EXISTENCE:mutant phenotype:GO:0015786 UDP-glucose transmembrane transport [PMID:18693752]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:10788474]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:10788474]
EXISTENCE:mutant phenotype:GO:1990569 UDP-N-acetylglucosamine transmembrane transport [PMID:10788474]
Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER
tubulin-binding prefolding complex subunit GIM4
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9463374]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:9463374]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9463374]
EXISTENCE:physical interaction:GO:0016272 prefoldin complex [PMID:9463374]
Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
dolichyl-diphosphooligosaccharide-protein glycotransferase
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:1724755]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12860997|PMID:1724755]
EXISTENCE:direct assay:GO:0008250 oligosaccharyltransferase complex [PMID:10358084]
EXISTENCE:direct assay:GO:0016757 glycosyltransferase activity [PMID:1600939]
EXISTENCE:mutant phenotype:GO:0004576 oligosaccharyl transferase activity [PMID:1600939]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:12974639]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:9405463]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Non-catalytic beta subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes N-linked glycosylation of newly synthesized proteins in the ER; forms a pocket with Ost2p involved in binding terminal glucose units of the donor glycan; human homolog DDOST can complement yeast growth defect during down-regulation of the yeast gene
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:22842922|PMID:26928762]
hypothetical protein; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:7962051]
EXISTENCE:direct assay:GO:0006493 protein O-linked glycosylation [PMID:7962051]
EXISTENCE:mutant phenotype:GO:0000033 alpha-1,3-mannosyltransferase activity [PMID:8146181]
EXISTENCE:mutant phenotype:GO:0006491 N-glycan processing [PMID:8146181]
Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides; one of five S. cerevisiae proteins of MNN1 family; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:11914276]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:11583614]
Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
EXISTENCE:direct assay:GO:0004476 mannose-6-phosphate isomerase activity [PMID:8011630]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:1377774]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:1377774]
EXISTENCE:mutant phenotype:GO:0009298 GDP-mannose biosynthetic process [PMID:1377774]
Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
hypothetical protein; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10551827]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10409709]
EXISTENCE:direct assay:GO:0017110 nucleoside diphosphate phosphatase activity [PMID:10409709]
EXISTENCE:direct assay:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:10409709]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:10409709]
Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:16803892]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16803892]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247|PMID:26928762]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10684247|PMID:16803892]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:11583615]
EXISTENCE:genetic interaction:GO:0030687 preribosome, large subunit precursor [PMID:16803892]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:11583615]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12089522]
GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:9215891]
EXISTENCE:genetic interaction:GO:0007021 tubulin complex assembly [PMID:9215891]
EXISTENCE:mutant phenotype:GO:0007021 tubulin complex assembly [PMID:9215891]
EXISTENCE:physical interaction:GO:0043014 alpha-tubulin binding [PMID:9885248]
Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
translation machinery-associated protein 20
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:23613772]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:16702403]
hypothetical protein that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:2962902]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10449419|PMID:10377396]
EXISTENCE:physical interaction:GO:0017069 snRNA binding [PMID:2440583]
U4 spliceosomal RNA (U4 snRNA); interacts with U6
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11595741|PMID:9491896]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:8978675]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:18195105]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11595741|PMID:17717527|PMID:9491896|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11595741|PMID:9491896|PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0006887 exocytosis [PMID:15772160]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:11283608]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9491896|PMID:19053807]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:12913108|PMID:17717527]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:genetic interaction:GO:0048309 endoplasmic reticulum inheritance [PMID:15583030]
EXISTENCE:genetic interaction:GO:0051601 exocyst localization [PMID:17717527|PMID:18946089]
EXISTENCE:mutant phenotype:GO:0001927 exocyst assembly [PMID:15583030]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:12960429|PMID:15583030]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:11595741]
Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:8702493]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:10889207]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:8702493|PMID:11024003]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:8702493]
Nuclear envelope protein; interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
bifunctional 4-hydroxy-4-methyl-2-oxoglutarate aldolase/oxaloacetate decarboxylase
EXISTENCE:direct assay:GO:0008948 oxaloacetate decarboxylase activity [PMID:24359411]
EXISTENCE:direct assay:GO:0019619 3,4-dihydroxybenzoate catabolic process [PMID:24359411]
EXISTENCE:direct assay:GO:0047443 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity [PMID:24359411]
Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate
ARS511; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:9363789]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460|PMID:8733242]
EXISTENCE:direct assay:GO:0031505 fungal-type cell wall organization [PMID:9363789]
Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p; target of K. lactis zymocin
EXISTENCE:curator inference:GO:0034515 proteasome storage granule [PMID:9087403]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10419517]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15973433]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:mutant phenotype:GO:0061133 endopeptidase activator activity [PMID:8808631]
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
DEAH-box ATP-dependent RNA helicase PRP22
EXISTENCE:direct assay:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:12212850]
EXISTENCE:direct assay:GO:0000386 second spliceosomal transesterification activity [PMID:12212850]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:9582286]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:22408182]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:9524130]
EXISTENCE:mutant phenotype:GO:0000390 spliceosomal complex disassembly [PMID:9524130]
EXISTENCE:mutant phenotype:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:9524130]
EXISTENCE:mutant phenotype:GO:0071021 U2-type post-spliceosomal complex [PMID:12212850]
DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
oxygen-dependent protoporphyrinogen oxidase
EXISTENCE:direct assay:GO:0004729 oxygen-dependent protoporphyrinogen oxidase activity [PMID:7798202]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239|PMID:26928762]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7798202]
EXISTENCE:mutant phenotype:GO:0004729 oxygen-dependent protoporphyrinogen oxidase activity [PMID:7052077]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:7035824]
Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:11452010]
Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
EXISTENCE:direct assay:GO:0004467 long-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:8670886]
EXISTENCE:direct assay:GO:0031956 medium-chain fatty acid-CoA ligase activity [PMID:8206942]
EXISTENCE:genetic interaction:GO:0001676 long-chain fatty acid metabolic process [PMID:7962057]
EXISTENCE:genetic interaction:GO:0031957 very long-chain fatty acid-CoA ligase activity [PMID:9988704]
EXISTENCE:mutant phenotype:GO:0001676 long-chain fatty acid metabolic process [PMID:7962057]
Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:10352017]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:10352017]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:10352017]
EXISTENCE:direct assay:GO:0031116 positive regulation of microtubule polymerization [PMID:20392838]
EXISTENCE:direct assay:GO:0035371 microtubule plus-end [PMID:20392838]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:20392838]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:10352017]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714]
EXISTENCE:genetic interaction:GO:0030543 2-micrometer plasmid partitioning [PMID:28004422]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:11285282]
EXISTENCE:mutant phenotype:GO:0007019 microtubule depolymerization [PMID:10352017]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:33752231]
EXISTENCE:mutant phenotype:GO:0007026 negative regulation of microtubule depolymerization [PMID:10352017]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:14742714]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:10352017]
EXISTENCE:mutant phenotype:GO:0030543 2-micrometer plasmid partitioning [PMID:28004422]
EXISTENCE:mutant phenotype:GO:1904825 protein localization to microtubule plus-end [PMID:29576319]
Microtubule plus end-tracking protein; forms a complex with Kar9p that makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1)
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23509072]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:7926051]
EXISTENCE:direct assay:GO:0005745 m-AAA complex [PMID:8681382]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:19748354]
EXISTENCE:direct assay:GO:0097002 mitochondrial inner boundary membrane [PMID:19019989]
EXISTENCE:mutant phenotype:GO:0006465 signal peptide processing [PMID:12417197]
EXISTENCE:mutant phenotype:GO:0008237 metallopeptidase activity [PMID:8681382]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:19748354]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:27398316]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:8681382]
Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
kinetochore-associated Ndc80 complex subunit SPC25
EXISTENCE:direct assay:GO:0031262 Ndc80 complex [PMID:11179222|PMID:11266451]
EXISTENCE:mutant phenotype:GO:0000776 kinetochore [PMID:11179222]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11266451]
EXISTENCE:mutant phenotype:GO:0098653 centromere clustering [PMID:11179222]
EXISTENCE:physical interaction:GO:0031262 Ndc80 complex [PMID:11179222]
Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
inositol phosphosphingolipid phospholipase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14699160]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:17880915]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14699160|PMID:26928762]
EXISTENCE:direct assay:GO:0030149 sphingolipid catabolic process [PMID:11006294]
EXISTENCE:direct assay:GO:0052712 inositol phosphosphingolipid phospholipase activity [PMID:11006294]
EXISTENCE:mutant phenotype:GO:0009651 response to salt stress [PMID:12180980]
EXISTENCE:mutant phenotype:GO:0046513 ceramide biosynthetic process [PMID:17880915|PMID:23620586]
EXISTENCE:mutant phenotype:GO:0090266 regulation of mitotic cell cycle spindle assembly checkpoint [PMID:32205408]
Inositol phosphosphingolipid phospholipase C; hydrolyzes complex sphingolipids to produce ceramide; regulates the spindle assembly checkpoint upstream of PP2A-Cdc55; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; mediates Na+ and Li+ halotolerance; activated by phosphatidylserine, cardiolipin and phosphatidylglycerol; mitochondrial outer membrane protein; ortholog of mammalian neutral sphingomyelinase type 2
Arf family guanine nucleotide exchange factor SBH2
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:12750387]
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:27831485]
EXISTENCE:direct assay:GO:0071261 Ssh1 translocon complex [PMID:8612571]
EXISTENCE:genetic interaction:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:18347066]
Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication
guanine nucleotide-binding protein subunit alpha
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16924114]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:31690628]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16030250|PMID:31690628|PMID:16622836]
EXISTENCE:direct assay:GO:0010737 protein kinase A signaling [PMID:31690628]
EXISTENCE:genetic interaction:GO:0010255 glucose mediated signaling pathway [PMID:10361302|PMID:2830616]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:9384580]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:10454558|PMID:10655215|PMID:9384580]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:10454558]
EXISTENCE:mutant phenotype:GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway [PMID:9628870]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0009757 hexose mediated signaling [PMID:10655215]
EXISTENCE:mutant phenotype:GO:0010255 glucose mediated signaling pathway [PMID:2830616|PMID:9628870]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:10454558]
EXISTENCE:physical interaction:GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway [PMID:16924114]
Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus
proteasome regulatory particle lid subunit RPN3
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:9741626]
Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:23447536]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8187178]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:direct assay:GO:0140297 DNA-binding transcription factor binding [PMID:9660972]
EXISTENCE:mutant phenotype:GO:0001139 RNA polymerase II complex recruiting activity [PMID:19805365]
EXISTENCE:mutant phenotype:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:16263706]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:33789343]
EXISTENCE:physical interaction:GO:0001139 RNA polymerase II complex recruiting activity [PMID:21415355]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7009582]
EXISTENCE:mutant phenotype:GO:0004735 pyrroline-5-carboxylate reductase activity [PMID:1508147|PMID:2824433]
EXISTENCE:mutant phenotype:GO:0006561 proline biosynthetic process [PMID:1508147|PMID:2824433]
Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18427809]
EXISTENCE:mutant phenotype:GO:0004092 carnitine O-acetyltransferase activity [PMID:11329169]
EXISTENCE:mutant phenotype:GO:0006066 alcohol metabolic process [PMID:11329169]
EXISTENCE:mutant phenotype:GO:0009437 carnitine metabolic process [PMID:11329169]
Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
translation initiation factor eIF2 subunit gamma
EXISTENCE:direct assay:GO:0001731 formation of translation preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14698289|PMID:16246727]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:22683627]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:11018020|PMID:15838098]
EXISTENCE:direct assay:GO:1990856 methionyl-initiator methionine tRNA binding [PMID:16565414]
EXISTENCE:mutant phenotype:GO:0000049 tRNA binding [PMID:8947054]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:23063529]
EXISTENCE:mutant phenotype:GO:0005525 GTP binding [PMID:8947054]
EXISTENCE:mutant phenotype:GO:0005850 eukaryotic translation initiation factor 2 complex [PMID:8417348]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:23063529]
EXISTENCE:mutant phenotype:GO:0045903 positive regulation of translational fidelity [PMID:9308967]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:22683627]
Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID) syndrome
Histidine tRNA (tRNA-His), predicted by tRNAscan-SE analysis
CDP-diacylglycerol-serine O-phosphatidyltransferase
EXISTENCE:direct assay:GO:0003882 CDP-diacylglycerol-serine O-phosphatidyltransferase activity [PMID:6088519]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:3005242]
EXISTENCE:direct assay:GO:0006659 phosphatidylserine biosynthetic process [PMID:6088519]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:6274497]
EXISTENCE:mutant phenotype:GO:0003882 CDP-diacylglycerol-serine O-phosphatidyltransferase activity [PMID:6988386]
EXISTENCE:mutant phenotype:GO:0006659 phosphatidylserine biosynthetic process [PMID:6988386|PMID:3031455]
Phosphatidylserine synthase; functions in phospholipid biosynthesis; transcriptionally repressed by myo-inositol and choline
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11331606|PMID:17237508]
EXISTENCE:direct assay:GO:0005641 nuclear envelope lumen [PMID:17237508]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17237508]
EXISTENCE:direct assay:GO:0031588 nucleotide-activated protein kinase complex [PMID:12393914]
EXISTENCE:genetic interaction:GO:0004679 AMP-activated protein kinase activity [PMID:10990457]
EXISTENCE:genetic interaction:GO:0007165 signal transduction [PMID:10990457]
EXISTENCE:genetic interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:9121458]
EXISTENCE:genetic interaction:GO:0140767 enzyme-substrate adaptor activity [PMID:27592031]
EXISTENCE:genetic interaction:GO:2000222 positive regulation of pseudohyphal growth [PMID:16980405]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12556493]
EXISTENCE:mutant phenotype:GO:0004679 AMP-activated protein kinase activity [PMID:7813428]
EXISTENCE:mutant phenotype:GO:0007155 cell adhesion [PMID:12556493]
EXISTENCE:physical interaction:GO:0031588 nucleotide-activated protein kinase complex [PMID:7813428|PMID:9121458]
One of three alternate beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functionally redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:19680226]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12239282|PMID:9832517]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19680226]
EXISTENCE:genetic interaction:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:9832517]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:14993292]
EXISTENCE:genetic interaction:GO:0034644 cellular response to UV [PMID:19680226]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:19680226]
EXISTENCE:mutant phenotype:GO:0000430 regulation of transcription from RNA polymerase II promoter by glucose [PMID:23275883]
EXISTENCE:mutant phenotype:GO:0045471 response to ethanol [PMID:23275883]
Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes
ARS512; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284|PMID:11720285]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
Core Sm protein Sm B; part of heteroheptameric complex (with Smd1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm B and Sm B'
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:17289584]
EXISTENCE:mutant phenotype:GO:2001168 positive regulation of histone H2B ubiquitination [PMID:20008511]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:17289584]
EXISTENCE:physical interaction:GO:0042393 histone binding [PMID:17289584]
Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:11210571]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15537705]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15537705]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:26906739]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:15537705]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:9151665]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:9151665]
Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095]
EXISTENCE:genetic interaction:GO:0006378 mRNA polyadenylation [PMID:16788068]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:9196079]
EXISTENCE:mutant phenotype:GO:0032465 regulation of cytokinesis [PMID:30455324]
EXISTENCE:mutant phenotype:GO:0043631 RNA polyadenylation [PMID:16788068]
Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:15972461]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15972461]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:15972461]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15972461]
hypothetical protein; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
hypothetical protein; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:12554867]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:12554867]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19682251|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19682251]
EXISTENCE:mutant phenotype:GO:0032056 positive regulation of translation in response to stress [PMID:19682251]
RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16260602]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:16260602]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:16260602]
EXISTENCE:mutant phenotype:GO:0042254 ribosome biogenesis [PMID:16260602]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:32320318]
ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; important for disaggregation of ordered and disordered aggregates; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant
bifunctional nucleotidase/lysophosphatidic acid phosphatase
EXISTENCE:direct assay:GO:0008252 nucleotidase activity [PMID:23670538]
EXISTENCE:direct assay:GO:0052642 lysophosphatidic acid phosphatase activity [PMID:18234677]
EXISTENCE:mutant phenotype:GO:0009166 nucleotide catabolic process [PMID:23670538]
EXISTENCE:mutant phenotype:GO:0016036 cellular response to phosphate starvation [PMID:18234677]
EXISTENCE:mutant phenotype:GO:0052642 lysophosphatidic acid phosphatase activity [PMID:18234677]
EXISTENCE:mutant phenotype:GO:0070328 triglyceride homeostasis [PMID:26979516]
EXISTENCE:mutant phenotype:GO:0090549 response to carbon starvation [PMID:23670538]
Lysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; repressed by Gcn4p under normal conditions; PHM8 has a paralog, SDT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:34191293]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391]
Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
Mitochondrial hypothetical protein; almost completely overlaps ORF HVG1/YER039C
hypothetical protein; HVG1 has a paralog, VRG4, that arose from the whole genome duplication
hypothetical protein; YER039C-A is not an essential gene
nitrogen-responsive transcriptional regulator GLN3
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7608102]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:8655543]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10604478|PMID:27293098]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12140287]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:7568152]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:8655543]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:2153652|PMID:26649943]
EXISTENCE:mutant phenotype:GO:2001159 regulation of protein localization by the Cvt pathway [PMID:26649943]
Transcriptional activator in nitrogen catabolite repression system; localization and activity regulated by quality of nitrogen source and Ure2p; full-length protein forms prion-like aggregates when overproduced
crossover junction endodeoxyribonuclease
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:34928393|PMID:19520826]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19520826]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:34928393]
EXISTENCE:direct assay:GO:0008821 crossover junction endodeoxyribonuclease activity [PMID:19020614]
EXISTENCE:direct assay:GO:0051908 double-stranded DNA 5'-3' exodeoxyribonuclease activity [PMID:34928393]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:20106725]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:20106725]
EXISTENCE:mutant phenotype:GO:0008821 crossover junction endodeoxyribonuclease activity [PMID:19020614]
Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p
peptide-methionine-S-sulfoxide reductase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20799725|PMID:14562095]
EXISTENCE:direct assay:GO:0008113 peptide-methionine (S)-S-oxide reductase activity [PMID:15141092|PMID:9275166]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:11929995]
EXISTENCE:mutant phenotype:GO:0008113 peptide-methionine (S)-S-oxide reductase activity [PMID:15141092|PMID:9275166]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11929995|PMID:9275166]
Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease
EXISTENCE:mutant phenotype:GO:0004013 adenosylhomocysteinase activity [PMID:18591246]
EXISTENCE:mutant phenotype:GO:0006641 triglyceride metabolic process [PMID:18591246]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:18591246]
EXISTENCE:mutant phenotype:GO:0019510 S-adenosylhomocysteine catabolic process [PMID:18591246]
S-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12119386]
EXISTENCE:direct assay:GO:0006696 ergosterol biosynthetic process [PMID:11160377]
EXISTENCE:mutant phenotype:GO:0006696 ergosterol biosynthetic process [PMID:10929718|PMID:29319811]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:12119386|PMID:15522820]
Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16783010|PMID:17558514]
EXISTENCE:genetic interaction:GO:0042138 meiotic DNA double-strand break formation [PMID:10224247]
Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10825197]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10825197]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:10825197]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10825197]
ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:26605945|PMID:27303688]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15590821]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:26605945|PMID:27303688]
Meiosis-specific protein required for prospore membrane morphogenesis; required for the proper shape of the prospore membrane (PSM) and for spore wall formation; functions cooperatively with SPO71 in PSM elongation; physically interacts with Spo71p; genetically antagonistic to SPO1, similar to SPO71; localizes to the PSM; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis; dysferlin domain-only protein
Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:32777224]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:32777224]
Nuclear type II J heat shock protein of the E. coli dnaJ family; overexpression stabilizes amino acid permeases; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly; localizes to the cytosol and plasma membrane, while a GFP fusion protein localizes to the nucleus
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:16341089|PMID:16341090]
EXISTENCE:direct assay:GO:0031071 cysteine desulfurase activity [PMID:24045011]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:16341089|PMID:16341090]
EXISTENCE:physical interaction:GO:0016226 iron-sulfur cluster assembly [PMID:16341090]
EXISTENCE:physical interaction:GO:1990221 L-cysteine desulfurase complex [PMID:24045011]
Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:20040577]
EXISTENCE:direct assay:GO:0018188 peptidyl-proline di-hydroxylation [PMID:24550462|PMID:31320474]
EXISTENCE:direct assay:GO:0031543 peptidyl-proline dioxygenase activity [PMID:24550462|PMID:31320474]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:16809762]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:24550462|PMID:16809762|PMID:20630870]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:24550462]
EXISTENCE:mutant phenotype:GO:0008198 ferrous iron binding [PMID:25381260]
Prolyl hydroxylase; catalyzes oxygen-dependent dihydroxylation of Rps23a/b, a 40S ribosomal decoding center subunit; influences translational termination and regulates translational accuracy; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; disputed role as a oxidative dealkylase repairing DNA methyl-base lesions; poly(rA)-binding protein affecting poly(A) tail length and mRNA stability; Fe(II)/2-oxoglutarate-dependent dioxygenase family member similar to human prolyl 4-hydroxylase OGFOD1
mitochondrial 37S ribosomal protein RSM18
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:17142463]
EXISTENCE:direct assay:GO:0051864 histone H3K36 demethylase activity [PMID:16362057]
EXISTENCE:genetic interaction:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:17525156]
EXISTENCE:mutant phenotype:GO:0051864 histone H3K36 demethylase activity [PMID:17369256]
JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe
EXISTENCE:direct assay:GO:0004072 aspartate kinase activity [PMID:18626862]
EXISTENCE:direct assay:GO:0009088 threonine biosynthetic process [PMID:18626862]
EXISTENCE:mutant phenotype:GO:0004072 aspartate kinase activity [PMID:18626862]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:4380684]
EXISTENCE:mutant phenotype:GO:0009088 threonine biosynthetic process [PMID:4380684|PMID:18626862]
EXISTENCE:mutant phenotype:GO:0009090 homoserine biosynthetic process [PMID:4380684]
Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
ARS513; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:14756774]
EXISTENCE:direct assay:GO:0005375 copper ion transmembrane transporter activity [PMID:23846699]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14756774|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0035434 copper ion transmembrane transport [PMID:23846699]
EXISTENCE:direct assay:GO:0035435 phosphate ion transmembrane transport [PMID:14756774]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:23846699]
Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:8754819]
EXISTENCE:mutant phenotype:GO:0005977 glycogen metabolic process [PMID:11973298]
EXISTENCE:physical interaction:GO:0000164 protein phosphatase type 1 complex [PMID:8754819]
Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241]
EXISTENCE:mutant phenotype:GO:0003879 ATP phosphoribosyltransferase activity [PMID:14190241]
ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8267570|PMID:22842922]
EXISTENCE:direct assay:GO:0015205 nucleobase transmembrane transporter activity [PMID:9092500]
EXISTENCE:direct assay:GO:0015212 cytidine transmembrane transporter activity [PMID:10501935]
EXISTENCE:direct assay:GO:0015856 cytosine transport [PMID:9092500]
EXISTENCE:direct assay:GO:0015861 cytidine transport [PMID:10501935]
EXISTENCE:direct assay:GO:0072530 purine-containing compound transmembrane transport [PMID:9092500]
EXISTENCE:mutant phenotype:GO:0015212 cytidine transmembrane transporter activity [PMID:10501935]
EXISTENCE:mutant phenotype:GO:0015861 cytidine transport [PMID:10501935]
Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:23874617]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:23874617]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23874617]
EXISTENCE:direct assay:GO:0042254 ribosome biogenesis [PMID:23874617]
Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
putative isoleucine biosynthesis protein HMF1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11442631]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11442631]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11003673]
Member of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:27998984]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:8381337|PMID:27998984]
Assembly factor that couples heme a synthesis to complex IV assembly; regulates the oligomerization state of the Cox15p heme a synthase, and couples this to the assembly of the cytochrome C oxidase complex (complex IV)
EXISTENCE:genetic interaction:GO:0005979 regulation of glycogen biosynthetic process [PMID:11602261]
EXISTENCE:genetic interaction:GO:0005981 regulation of glycogen catabolic process [PMID:11602261]
Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0015205 nucleobase transmembrane transporter activity [PMID:16845689]
EXISTENCE:mutant phenotype:GO:0015856 cytosine transport [PMID:16845689]
Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function
ARS513.5; Very weak autonomously replicating sequence
ARS513.7; Weak autonomously replicating sequence
Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant
EXISTENCE:direct assay:GO:0000121 glycerol-1-phosphatase activity [PMID:8662716]
EXISTENCE:direct assay:GO:0006970 response to osmotic stress [PMID:8662716]
EXISTENCE:direct assay:GO:0044262 cellular carbohydrate metabolic process [PMID:8662716]
EXISTENCE:mutant phenotype:GO:0006114 glycerol biosynthetic process [PMID:11058591]
DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16738307]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:16738307]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:16738307]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:9707445]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:16738307]
EXISTENCE:mutant phenotype:GO:0022618 ribonucleoprotein complex assembly [PMID:16738307]
Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11342098]
Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8870676|PMID:9370278]
EXISTENCE:mutant phenotype:GO:0004451 isocitrate lyase activity [PMID:1551398]
EXISTENCE:mutant phenotype:GO:0006097 glyoxylate cycle [PMID:8878673]
Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
ARS514; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:9023104]
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
hypothetical protein; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:20567505]
EXISTENCE:mutant phenotype:GO:0006112 energy reserve metabolic process [PMID:20567505]
Protein involved in energy metabolism under respiratory conditions; abundance is increased upon intracellular iron depletion or in response to DNA replication stress; suppresses pro-apoptotic effect of expressing human BAX in yeast cells; RGI1 has a paralog, RGI2, that arose from the whole genome duplication
CCR4-NOT core ubiquitin-protein ligase subunit MOT2
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:19346402]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:16926149]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:19346402|PMID:16926149]
EXISTENCE:genetic interaction:GO:0010498 proteasomal protein catabolic process [PMID:24261871]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:19346402]
EXISTENCE:mutant phenotype:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:15706350]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:11404327]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:10490603]
Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication
bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase
EXISTENCE:direct assay:GO:0003942 N-acetyl-gamma-glutamyl-phosphate reductase activity [PMID:1851947]
EXISTENCE:direct assay:GO:0003991 acetylglutamate kinase activity [PMID:1851947]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:1851947]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:15486299]
Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
ribonucleotide-diphosphate reductase subunit RNR1
EXISTENCE:direct assay:GO:0000166 nucleotide binding [PMID:16537479]
EXISTENCE:direct assay:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:10535923|PMID:10716984|PMID:11893751]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12732713|PMID:9315670|PMID:22842922]
EXISTENCE:direct assay:GO:0005971 ribonucleoside-diphosphate reductase complex [PMID:10716984]
EXISTENCE:direct assay:GO:0009263 deoxyribonucleotide biosynthetic process [PMID:10535923|PMID:5459124]
EXISTENCE:mutant phenotype:GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor [PMID:8552025]
Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:28706108|PMID:29467252]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:29467252]
EXISTENCE:physical interaction:GO:0030837 negative regulation of actin filament polymerization [PMID:29467252]
EXISTENCE:physical interaction:GO:0110131 Aim21-Tda2 complex [PMID:28706108|PMID:29467252]
Subunit of a complex that associates with actin filaments; forms a complex with Aim21p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; TcTex1-type dynein light chain family member; null mutant is sensitive to expression of the top1-T722A allele
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:12584253]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17079729]
EXISTENCE:direct assay:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:direct assay:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:27831485]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:11102525|PMID:19390046]
EXISTENCE:mutant phenotype:GO:0006799 polyphosphate biosynthetic process [PMID:27252384]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:17079729]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:11823419|PMID:27252384]
EXISTENCE:physical interaction:GO:0033254 vacuolar transporter chaperone complex [PMID:11823419]
Regulatory subunit of vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; also binds mRNA; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004029 aldehyde dehydrogenase (NAD+) activity [PMID:9473035]
EXISTENCE:direct assay:GO:0004030 aldehyde dehydrogenase [NAD(P)+] activity [PMID:9473035]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10585550|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0019413 acetate biosynthetic process [PMID:15256563]
Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:15659647]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15659647]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:15659647]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:15659647]
EXISTENCE:physical interaction:GO:0000139 Golgi membrane [PMID:15659647]
EXISTENCE:physical interaction:GO:0005789 endoplasmic reticulum membrane [PMID:15659647]
EXISTENCE:physical interaction:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:15659647]
Integral membrane protein required for ER to Golgi transport; localized to the Golgi, the ER, and COPII vesicles; interacts with Yip1p and Yif1p
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:9224718]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17952059]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17952059|PMID:10523653]
EXISTENCE:direct assay:GO:0035335 peptidyl-tyrosine dephosphorylation [PMID:9224718]
EXISTENCE:genetic interaction:GO:0035335 peptidyl-tyrosine dephosphorylation [PMID:9224718|PMID:10523653]
EXISTENCE:genetic interaction:GO:0043937 regulation of sporulation [PMID:10679022]
EXISTENCE:genetic interaction:GO:0071507 pheromone response MAPK cascade [PMID:9224718]
EXISTENCE:genetic interaction:GO:0071852 fungal-type cell wall organization or biogenesis [PMID:10523653]
EXISTENCE:genetic interaction:GO:1903138 negative regulation of cell wall integrity MAPK cascade [PMID:10523653]
EXISTENCE:mutant phenotype:GO:0035335 peptidyl-tyrosine dephosphorylation [PMID:9224718]
EXISTENCE:mutant phenotype:GO:0051220 cytoplasmic sequestering of protein [PMID:10817757]
Phosphotyrosine-specific protein phosphatase; major role in the pheromone adaptive response through dephosphorylation of the Fus3p MAPK with a minor role by Ptp2p and Msg5p; minor role in the inactivation of Hog1p MAPK during osmolarity sensing with major role by Ptp2p; dephosphorylates and regulates the localization of Hog1p; minor role in the cell wall integrity pathway through tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p plays a major role; localizes to the cytoplasm
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239]
Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
ARS515; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004177 aminopeptidase activity [PMID:19720832]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19720832]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19720832|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0016485 protein processing [PMID:19837041]
EXISTENCE:mutant phenotype:GO:0004177 aminopeptidase activity [PMID:19720832|PMID:19837041]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:19720832|PMID:19837041]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:19837041]
Mitochondrial aminopeptidase; cleaves N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of aminopeptidase P family; exists in rapid equilibrium between monomer and dimer, with dimer being active species; ortholog of human gene XPNPEP3, which is mutated in rare hereditary kidney disease similar to nephronophthisis
hypothetical protein; identified by fungal homology and RT-PCR
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:15175754]
EXISTENCE:expression pattern:GO:0045892 negative regulation of DNA-templated transcription [PMID:15175754]
EXISTENCE:mutant phenotype:GO:0045892 negative regulation of DNA-templated transcription [PMID:15175754]
Non-protein-coding RNA that regulates the transcription of SER3; expression of SRG1 RNA represses expression of its neighboring gene SER3 via a transcription-interference mechanism
EXISTENCE:direct assay:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
EXISTENCE:genetic interaction:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
EXISTENCE:mutant phenotype:GO:0004617 phosphoglycerate dehydrogenase activity [PMID:12525494]
EXISTENCE:mutant phenotype:GO:0009070 serine family amino acid biosynthetic process [PMID:12525494]
EXISTENCE:mutant phenotype:GO:0061759 alpha-ketoglutarate reductase activity [PMID:26774271]
3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309|PMID:12150911]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:15167894]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292|PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292|PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292|PMID:15590835]
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:genetic interaction:GO:0043495 protein-membrane adaptor activity [PMID:18724936]
EXISTENCE:genetic interaction:GO:0043529 GET complex [PMID:16269340]
EXISTENCE:genetic interaction:GO:0045048 protein insertion into ER membrane [PMID:18724936]
EXISTENCE:mutant phenotype:GO:0000423 mitophagy [PMID:29673596]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
hypothetical protein; expressed at both mRNA and protein levels
EXISTENCE:genetic interaction:GO:0006567 threonine catabolic process [PMID:7042346]
EXISTENCE:mutant phenotype:GO:0004794 L-threonine ammonia-lyase activity [PMID:5345980]
EXISTENCE:mutant phenotype:GO:0009097 isoleucine biosynthetic process [PMID:5345980]
Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
Arf family guanine nucleotide exchange factor SBH1
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:27831485]
EXISTENCE:direct assay:GO:0005784 Sec61 translocon complex [PMID:7758110|PMID:8612571]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:31201345]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0015450 protein-transporting ATPase activity [PMID:7758110]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:19857245]
Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; cotranslationally N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:9755194]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:9755194]
Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:10580002|PMID:9528768]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12477803|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12477803|PMID:14562095]
EXISTENCE:direct assay:GO:0035970 peptidyl-threonine dephosphorylation [PMID:12477803]
EXISTENCE:direct assay:GO:1990439 MAP kinase serine/threonine phosphatase activity [PMID:12477803]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:10580002]
EXISTENCE:mutant phenotype:GO:1900102 negative regulation of endoplasmic reticulum unfolded protein response [PMID:9528768]
EXISTENCE:physical interaction:GO:1900102 negative regulation of endoplasmic reticulum unfolded protein response [PMID:9528768]
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004049 anthranilate synthase activity [PMID:3881257]
EXISTENCE:genetic interaction:GO:0004049 anthranilate synthase activity [PMID:6323449]
EXISTENCE:mutant phenotype:GO:0000162 tryptophan biosynthetic process [PMID:8432699]
EXISTENCE:physical interaction:GO:0005950 anthranilate synthase complex [PMID:3881257]
Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase
EXISTENCE:mutant phenotype:GO:0003871 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity [PMID:3542720]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:1101032]
Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination
EXISTENCE:curator inference:GO:0031929 TOR signaling [PMID:12408816]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:30969890]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:15809876]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9804843]
EXISTENCE:mutant phenotype:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:15809876]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15809876]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:30969890]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:16002396]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:30969890]
EXISTENCE:physical interaction:GO:0001558 regulation of cell growth [PMID:12408816]
EXISTENCE:physical interaction:GO:0030950 establishment or maintenance of actin cytoskeleton polarity [PMID:12408816]
EXISTENCE:physical interaction:GO:0031932 TORC2 complex [PMID:12408816]
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; contains an N-terminal armadillo repeat region necessary for recruitment to the PM and for anchoring Tor2p and Avo1p at the PM; acts along with Avo1p as a scaffold important for integrity of the TORC2 complex; involved in sphingolipid metabolism; contains a RasGEFN domain
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
hypothetical protein; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; localizes to the peroxisome in glucose; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9087403]
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:9087403]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:genetic interaction:GO:0061133 endopeptidase activator activity [PMID:9207060]
Beta 3 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit C10
EXISTENCE:direct assay:GO:0000150 DNA strand exchange activity [PMID:8066464]
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11459983]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9427283]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:26540255]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:32083180]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:20371520|PMID:32083180]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:26540255]
EXISTENCE:direct assay:GO:0006310 DNA recombination [PMID:22955832]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:8066464]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:10506208]
EXISTENCE:direct assay:GO:0042148 strand invasion [PMID:8066464|PMID:9271413]
EXISTENCE:mutant phenotype:GO:0000709 meiotic joint molecule formation [PMID:1581961]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:25822194]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:32083180]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:7026973]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:7026973]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:16581767]
EXISTENCE:mutant phenotype:GO:0043504 mitochondrial DNA repair [PMID:26540255]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:31142613]
Strand exchange protein; forms helical filament with DNA that searches for homology; involved in recombinational repair of DNA DSBs during vegetative growth and meiosis; phosphorylation by Cdc28p in G2/M phase promotes DNA binding, strand invasion, and primer extension; L1 and L2 motifs of DNA binding site I have critical roles in ensuring DNA fidelity during meiotic recombination; RAD51 and RAD4 pathways confer resistance to benzo[a]pyrene dihydrodiol; homolog of Dmc1p and bacterial RecA
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:15755916]
EXISTENCE:direct assay:GO:0030437 ascospore formation [PMID:11918806]
EXISTENCE:genetic interaction:GO:0006031 chitin biosynthetic process [PMID:11918806]
EXISTENCE:genetic interaction:GO:0008047 enzyme activator activity [PMID:11918806]
Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
ARS516; Autonomously Replicating Sequence
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
putative ubiquitin-specific protease UBP9
Putative ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
ribose phosphate diphosphokinase subunit PRS2
EXISTENCE:direct assay:GO:0002189 ribose phosphate diphosphokinase complex [PMID:15280369]
EXISTENCE:genetic interaction:GO:0004749 ribose phosphate diphosphokinase activity [PMID:15280369]
EXISTENCE:genetic interaction:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:15280369]
EXISTENCE:mutant phenotype:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:10212224]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:15470112]
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:8396728]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8396728]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:8396728]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:8396728|PMID:8641272|PMID:8781238]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:19345192]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:18191224|PMID:8396728]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:8396728]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:18191224|PMID:8393731|PMID:8641272|PMID:8781238]
Ubiquitin-conjugating enzyme involved in ERAD; located at the cytosolic side of the ER membrane; tail region contains a transmembrane segment at the C-terminus; substrate of the ubiquitin-proteasome pathway; ER-associated protein degradation is also known as ERAD
EXISTENCE:genetic interaction:GO:0006612 protein targeting to membrane [PMID:7822420]
Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:18782943]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8B has a paralog, RPS8A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11279056]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11279056]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:9789005]
EXISTENCE:genetic interaction:GO:0051082 unfolded protein binding [PMID:9789005]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:8754838]
Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:30065069]
EXISTENCE:direct assay:GO:0140597 protein carrier chaperone [PMID:30065069]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:30065069]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
Chaperone for Replication Protein A complex (RPA); involved in nuclear import of RPA and its binding to ssDNA at replication forks; has a role in regulation of Ty1 transposition
EXISTENCE:curator inference:GO:0006913 nucleocytoplasmic transport [PMID:10684247]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:24062435]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:24062435]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0044611 nuclear pore inner ring [PMID:18046406]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:19414608]
EXISTENCE:genetic interaction:GO:0051292 nuclear pore complex assembly [PMID:19414608]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20098417|PMID:20932479]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:12663816|PMID:11163190|PMID:23861669]
EXISTENCE:direct assay:GO:0033551 monopolin complex [PMID:12689592]
EXISTENCE:direct assay:GO:0045859 regulation of protein kinase activity [PMID:27491543]
EXISTENCE:genetic interaction:GO:0010789 meiotic sister chromatid cohesion involved in meiosis I [PMID:15062096]
EXISTENCE:genetic interaction:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:23861669]
EXISTENCE:mutant phenotype:GO:0010789 meiotic sister chromatid cohesion involved in meiosis I [PMID:15062096]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0045143 homologous chromosome segregation [PMID:11163190]
EXISTENCE:mutant phenotype:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:11163190]
Monopolin; meiosis-specific kinetochore-associated protein involved in monopolar attachment of sister kinetochores to the meiotic spindle; subunit of monopolin, a complex that prevents biorientation of sister kinetochores to ensure homolog biorientation during meiosis I; regulates the conformation, enzyme kinetics and substrate specificity of the Dsn1p kinase, Hrr1p; expressed only during the first meiotic division
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:8970155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15210706]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:8970155]
EXISTENCE:mutant phenotype:GO:0032185 septin cytoskeleton organization [PMID:28776765]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:9725905]
EXISTENCE:mutant phenotype:GO:2000728 regulation of mRNA export from nucleus in response to heat stress [PMID:15210706]
RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12024012]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10515942]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12024012|PMID:8913742|PMID:11914276]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12024012]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10064592|PMID:12024012|PMID:10591965|PMID:16568252|P MID:10373537]
EXISTENCE:mutant phenotype:GO:1900189 positive regulation of cell adhesion involved in single-species biofilm formation [PMID:18202364]
Transcription factor; required for flocculation, diploid filamentous growth, and haploid invasive growth; forms a heterodimer with Mss11p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1; S. cerevisiae genome reference strain S288C contains an internal in-frame stop at codon 142, which in other strains encodes tryptophan
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9238021|PMID:10684247]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9238021|PMID:10684247]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:9321403]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:11694505]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:0061608 nuclear import signal receptor activity [PMID:9321403]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:11352936]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:11694505]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:9321403]
EXISTENCE:mutant phenotype:GO:2000220 regulation of pseudohyphal growth [PMID:22043304]
EXISTENCE:physical interaction:GO:0061608 nuclear import signal receptor activity [PMID:9182759]
Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0033309 SBF transcription complex [PMID:1832338|PMID:2689885|PMID:8423776]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:2649246|PMID:8423776]
EXISTENCE:mutant phenotype:GO:0033309 SBF transcription complex [PMID:8423776]
EXISTENCE:mutant phenotype:GO:0061408 positive regulation of transcription from RNA polymerase II promoter in response to heat stress [PMID:20641022]
EXISTENCE:mutant phenotype:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:2649246|PMID:3542227]
EXISTENCE:physical interaction:GO:0033309 SBF transcription complex [PMID:1465410]
DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18611963]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:10369684|PMID:11720284]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15075370]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:10369684]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:genetic interaction:GO:0033962 P-body assembly [PMID:17984320|PMID:18981231]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10428970]
EXISTENCE:mutant phenotype:GO:0017070 U6 snRNA binding [PMID:10369684]
EXISTENCE:physical interaction:GO:0005732 sno(s)RNA-containing ribonucleoprotein complex [PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:genetic interaction:GO:0006878 cellular copper ion homeostasis [PMID:20681974]
EXISTENCE:genetic interaction:GO:0007034 vacuolar transport [PMID:20526336]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:18178563]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:18178563]
Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:28751498]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12097146]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12097146]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:12097146]
EXISTENCE:genetic interaction:GO:0000920 septum digestion after cytokinesis [PMID:16615892]
EXISTENCE:genetic interaction:GO:0007015 actin filament organization [PMID:31964708]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:17417630]
EXISTENCE:mutant phenotype:GO:0099500 vesicle fusion to plasma membrane [PMID:28751498]
Protein involved in polar growth; functionally redundant with Boi1p; required for fusion of secretory vesicles with plasma membrane at sites of polarized growth and for inhibition of membrane abscission until the end of cytokinesis; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
EXISTENCE:expression pattern:GO:0030437 ascospore formation [PMID:2253272]
hypothetical protein; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
SUMO-targeted ubiquitin ligase complex subunit SLX8
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:26906737]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:17728242|PMID:17848550|PMID:18032921|PMID:26906737]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19270524|PMID:26906737|PMID:22842922]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:17728242|PMID:17848550|PMID:18032921|PMID:26906737]
EXISTENCE:direct assay:GO:0033768 SUMO-targeted ubiquitin ligase complex [PMID:17848550]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495]
EXISTENCE:genetic interaction:GO:0016567 protein ubiquitination [PMID:26906737]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:16387868]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:16428246]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495|PMID:16325482]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:26906737]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:16387868]
Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10980703]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:10980703]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10980703|PMID:26928762]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12374868]
EXISTENCE:genetic interaction:GO:0007231 osmosensory signaling pathway [PMID:7624781]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:19439450]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:9744864|PMID:18591427]
EXISTENCE:mutant phenotype:GO:0005078 MAP-kinase scaffold activity [PMID:15200958]
EXISTENCE:physical interaction:GO:0005034 osmosensor activity [PMID:7624781]
EXISTENCE:physical interaction:GO:0005078 MAP-kinase scaffold activity [PMID:15200958|PMID:16778768]
EXISTENCE:physical interaction:GO:0007231 osmosensory signaling pathway [PMID:7624781]
EXISTENCE:physical interaction:GO:0044697 HICS complex [PMID:22623719]
Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11274162]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20118605|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005313 L-glutamate transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0015183 L-aspartate transmembrane transporter activity [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0032974 amino acid transmembrane export from vacuole [PMID:11274162]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:11274162]
Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication
phosphatidylinositol-binding protein SCS2
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12727870|PMID:25420878]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12727870|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9537365]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25420878]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:17984322]
EXISTENCE:direct assay:GO:0031965 nuclear membrane [PMID:9537365]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:17963691]
EXISTENCE:direct assay:GO:0061163 endoplasmic reticulum polarization [PMID:25083872]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:25420878]
EXISTENCE:genetic interaction:GO:0008654 phospholipid biosynthetic process [PMID:8537323]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:11333225|PMID:12399377]
EXISTENCE:genetic interaction:GO:0048309 endoplasmic reticulum inheritance [PMID:17984322]
EXISTENCE:genetic interaction:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:23237950]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:genetic interaction:GO:0090158 endoplasmic reticulum membrane organization [PMID:23237950]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:12761300|PMID:9537365]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11333225]
EXISTENCE:mutant phenotype:GO:0032377 regulation of intracellular lipid transport [PMID:12727870]
EXISTENCE:mutant phenotype:GO:0033149 FFAT motif binding [PMID:12727870]
EXISTENCE:mutant phenotype:GO:0042308 negative regulation of protein import into nucleus [PMID:12727870]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:17984322]
EXISTENCE:physical interaction:GO:0005935 cellular bud neck [PMID:25083872]
EXISTENCE:physical interaction:GO:0033149 FFAT motif binding [PMID:12727870]
EXISTENCE:physical interaction:GO:0042308 negative regulation of protein import into nucleus [PMID:12727870]
Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
hypothetical protein; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy
ADP-ribosylation factor GTPase-activating protein
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:9927415]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9927415]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:15254269]
EXISTENCE:direct assay:GO:0048205 COPI coating of Golgi vesicle [PMID:15254269]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9927415]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9927415]
EXISTENCE:mutant phenotype:GO:0048205 COPI coating of Golgi vesicle [PMID:15254269]
EXISTENCE:physical interaction:GO:0030126 COPI vesicle coat [PMID:15254269]
ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:16301533]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14668479|PMID:24390141|PMID:26928762]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8816449]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8816449]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:19116312]
Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; targeted to vacuole via AP-3 pathway; regulates vesicle fusion in AP-3 pathway
ARS517; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0031683 G-protein beta/gamma-subunit complex binding [PMID:19820940]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:11309124]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:19820940]
EXISTENCE:mutant phenotype:GO:0010969 regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:19820940]
Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
NEDD4 family E3 ubiquitin-protein ligase
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:9108033]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:21685393|PMID:23443663|PMID:27694912|PMID:9108033]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14608372]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14657247|PMID:15078904]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14657247]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:14608372]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:19176477|PMID:21685393]
EXISTENCE:direct assay:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:30361468]
EXISTENCE:direct assay:GO:0010008 endosome membrane [PMID:14657247]
EXISTENCE:direct assay:GO:0010994 free ubiquitin chain polymerization [PMID:19153599]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:23443663]
EXISTENCE:direct assay:GO:0034450 ubiquitin-ubiquitin ligase activity [PMID:19153599]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:15078904]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:19252184]
EXISTENCE:genetic interaction:GO:0006513 protein monoubiquitination [PMID:14761940|PMID:19176477]
EXISTENCE:genetic interaction:GO:0006808 regulation of nitrogen utilization [PMID:15247235]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:10366593]
EXISTENCE:genetic interaction:GO:0010794 regulation of dolichol biosynthetic process [PMID:18771750]
EXISTENCE:genetic interaction:GO:0010795 regulation of ubiquinone biosynthetic process [PMID:18771750]
EXISTENCE:genetic interaction:GO:0019220 regulation of phosphate metabolic process [PMID:18165238]
EXISTENCE:genetic interaction:GO:0032443 regulation of ergosterol biosynthetic process [PMID:18771750]
EXISTENCE:genetic interaction:GO:0032956 regulation of actin cytoskeleton organization [PMID:15855235]
EXISTENCE:genetic interaction:GO:0034517 ribophagy [PMID:18670191]
EXISTENCE:genetic interaction:GO:0051865 protein autoubiquitination [PMID:24069405]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:9108033|PMID:32800549]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:21685393|PMID:27694912|PMID:9108033]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:12399376]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:25344756|PMID:9614172]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:14761940|PMID:19176477|PMID:21685393|PMID:32800549]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:10366593]
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:12163175]
EXISTENCE:mutant phenotype:GO:0010793 regulation of mRNA export from nucleus [PMID:14608372]
EXISTENCE:mutant phenotype:GO:0010794 regulation of dolichol biosynthetic process [PMID:18771750]
EXISTENCE:mutant phenotype:GO:0010795 regulation of ubiquinone biosynthetic process [PMID:18771750]
EXISTENCE:mutant phenotype:GO:0010796 regulation of multivesicular body size [PMID:17182850]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0031384 regulation of initiation of mating projection growth [PMID:21685393]
EXISTENCE:mutant phenotype:GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process [PMID:24962567]
EXISTENCE:mutant phenotype:GO:0032443 regulation of ergosterol biosynthetic process [PMID:18771750]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17182849]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:12867034]
EXISTENCE:mutant phenotype:GO:0034644 cellular response to UV [PMID:10490634]
EXISTENCE:mutant phenotype:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:17344478]
EXISTENCE:mutant phenotype:GO:0045723 positive regulation of fatty acid biosynthetic process [PMID:11007476]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:12654912]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11007476]
EXISTENCE:mutant phenotype:GO:0048260 positive regulation of receptor-mediated endocytosis [PMID:11179425]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:17344478|PMID:31658248|PMID:32800549]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:21070972]
EXISTENCE:mutant phenotype:GO:2000203 regulation of ribosomal large subunit export from nucleus [PMID:14608372]
EXISTENCE:mutant phenotype:GO:2000232 regulation of rRNA processing [PMID:14608372]
EXISTENCE:mutant phenotype:GO:2000235 regulation of tRNA processing [PMID:14608372]
EXISTENCE:mutant phenotype:GO:2000238 regulation of tRNA export from nucleus [PMID:14608372]
EXISTENCE:physical interaction:GO:0000151 ubiquitin ligase complex [PMID:9931424]
EXISTENCE:physical interaction:GO:0009410 response to xenobiotic stimulus [PMID:12163175]
EXISTENCE:physical interaction:GO:0010793 regulation of mRNA export from nucleus [PMID:15713680]
EXISTENCE:physical interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:17182849]
EXISTENCE:physical interaction:GO:0032880 regulation of protein localization [PMID:12867034]
EXISTENCE:physical interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:15713680]
NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p and Put4p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:16861225|PMID:11583614|PMID:17443350]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16861225]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16861225]
Protein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9814757]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9814757]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:9814757]
Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:24567329]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21777356]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:21777356]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:24567329]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:21777356]
Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:12748292|PMID:12847291|PMID:16201971|PMID:27524664]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22289182]
EXISTENCE:genetic interaction:GO:0090329 regulation of DNA-templated DNA replication [PMID:9341678]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:12748292|PMID:16201971]
EXISTENCE:mutant phenotype:GO:0005979 regulation of glycogen biosynthetic process [PMID:15340085]
EXISTENCE:mutant phenotype:GO:1900180 regulation of protein localization to nucleus [PMID:15340085]
EXISTENCE:mutant phenotype:GO:2000220 regulation of pseudohyphal growth [PMID:19880754]
Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal growth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:24236068]
EXISTENCE:mutant phenotype:GO:0071252 cellular response to sulfur dioxide [PMID:31799324]
EXISTENCE:mutant phenotype:GO:0080135 regulation of cellular response to stress [PMID:31799324]
Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under all growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:25144938]
EXISTENCE:mutant phenotype:GO:0042255 ribosome assembly [PMID:27303706]
EXISTENCE:physical interaction:GO:0030686 90S preribosome [PMID:25144938]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication; human homolog can partially complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:3327689]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:3327689]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:28542199]
EXISTENCE:mutant phenotype:GO:1990882 rRNA acetylation [PMID:28542199]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567|PMID:11081632]
EXISTENCE:physical interaction:GO:0042134 rRNA primary transcript binding [PMID:3327689]
C/D box small nucleolar RNA (snoRNA); acts as antisense guide to target RNA base acetylation by Kre33p to cytosine 1280 in 18S rRNA (responsible for ac4C 1280); establishes extended bipartite complementarity around the cytosines targeted for acetylation, similar to pseudouridylation pocket formation by the H/ACA snoRNPs; base pairing between snR4 and 18S rRNA requires the putative helicase activity of Kre33p
EXISTENCE:genetic interaction:GO:0030437 ascospore formation [PMID:9383076]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:9383076]
Protein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:15546625]
EXISTENCE:genetic interaction:GO:0031167 rRNA methylation [PMID:15546625]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243|PMID:15546625]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243|PMID:15546625]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U2921 and small subunit (SSU) rRNA at position A420
type 1 serine/threonine-protein phosphatase catalytic subunit GLC7
EXISTENCE:direct assay:GO:0000164 protein phosphatase type 1 complex [PMID:8289829]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19948764]
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:10747092]
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:1660885]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23418575]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11412094]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10747092]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11412094]
EXISTENCE:direct assay:GO:0032153 cell division site [PMID:26771880]
EXISTENCE:genetic interaction:GO:0000076 DNA replication checkpoint signaling [PMID:19884341]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:29529242]
EXISTENCE:genetic interaction:GO:0008360 regulation of cell shape [PMID:12477789]
EXISTENCE:genetic interaction:GO:2000370 positive regulation of clathrin-dependent endocytosis [PMID:12356757]
EXISTENCE:mutant phenotype:GO:0000076 DNA replication checkpoint signaling [PMID:19884341]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:19884341]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:11973298]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:25730376]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:10072383]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:10072380]
EXISTENCE:mutant phenotype:GO:0007116 regulation of cell budding [PMID:10639337]
EXISTENCE:mutant phenotype:GO:0007346 regulation of mitotic cell cycle [PMID:23418575]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:10207049]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:9584086]
EXISTENCE:mutant phenotype:GO:0031297 replication fork processing [PMID:19884341]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19948764]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:10072380|PMID:7891699|PMID:8164671|PMID:9584086]
EXISTENCE:mutant phenotype:GO:0070873 regulation of glycogen metabolic process [PMID:1660885]
EXISTENCE:mutant phenotype:GO:1901901 regulation of protein localization to cell division site involved in cytokinesis [PMID:26771880]
EXISTENCE:mutant phenotype:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:26771880]
EXISTENCE:physical interaction:GO:0000164 protein phosphatase type 1 complex [PMID:19948764]
EXISTENCE:physical interaction:GO:0051321 meiotic cell cycle [PMID:8754819]
Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p
EXISTENCE:direct assay:GO:0003993 acid phosphatase activity [PMID:11601995]
EXISTENCE:direct assay:GO:0030946 protein tyrosine phosphatase activity, metal-dependent [PMID:11601995]
Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene
hypothetical protein; conserved among S. cerevisiae strains; YER135C is not an essential gene
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
ARS518; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005093 Rab GDP-dissociation inhibitor activity [PMID:8157010]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:8157010]
GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18418087]
EXISTENCE:direct assay:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:7925282]
EXISTENCE:direct assay:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:10921896]
EXISTENCE:mutant phenotype:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:1655273]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:7925282]
RNA subunit of the Signal Recognition Particle (SRP)
ARS519; Autonomously Replicating Sequence
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
YERCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
hypothetical protein; YER138W-A has a paralog, YBL107W-A, that arose from a single-locus duplication
RNA polymerase II subunit B1 CTD phosphatase RTR1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18408053]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18408053|PMID:14562095]
EXISTENCE:direct assay:GO:0008420 RNA polymerase II CTD heptapeptide repeat phosphatase activity [PMID:19394294]
EXISTENCE:direct assay:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:19394294]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:18408053]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:18408053]
EXISTENCE:mutant phenotype:GO:0070940 dephosphorylation of RNA polymerase II C-terminal domain [PMID:19394294]
EXISTENCE:physical interaction:GO:0006366 transcription by RNA polymerase II [PMID:18408053]
Dual specificity protein phosphatase; dephosphorylates T1 and S5 in C-terminal domain (CTD) of RNA polymerase II largest subunit, Rpo21p; autoregulates turnover of its mRNA; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:23275891]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9228094]
EXISTENCE:genetic interaction:GO:0006784 heme A biosynthetic process [PMID:11788607]
EXISTENCE:genetic interaction:GO:0016653 oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor [PMID:11788607]
EXISTENCE:mutant phenotype:GO:0006784 heme A biosynthetic process [PMID:11788607]
EXISTENCE:mutant phenotype:GO:0016653 oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor [PMID:26940873|PMID:11248251]
Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:2265619]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:18472311]
EXISTENCE:direct assay:GO:0003905 alkylbase DNA N-glycosylase activity [PMID:2682633|PMID:2265619]
EXISTENCE:direct assay:GO:0008725 DNA-3-methyladenine glycosylase activity [PMID:18472311]
EXISTENCE:genetic interaction:GO:0006285 base-excision repair, AP site formation [PMID:18043759]
EXISTENCE:mutant phenotype:GO:0006285 base-excision repair, AP site formation [PMID:18043759]
EXISTENCE:mutant phenotype:GO:0006307 DNA dealkylation involved in DNA repair [PMID:2265620]
3-methyl-adenine DNA glycosylase; initiates base excision repair (BER) by removing damaged bases to create abasic sites that are subsequently repaired; expansion of BER repair removes alkylated bases resulting from alkylating agent-induced DNA damage, compensating for the lack of DNA repair by oxidative dealkylation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:10330187]
EXISTENCE:direct assay:GO:0004190 aspartic-type endopeptidase activity [PMID:32193351]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10330187]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:15964793]
EXISTENCE:direct assay:GO:0031593 polyubiquitin modification-dependent protein binding [PMID:32193351]
EXISTENCE:direct assay:GO:1904855 proteasome regulatory particle binding [PMID:15964793]
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0004190 aspartic-type endopeptidase activity [PMID:32193351]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:12051757]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:21094643]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:10330187]
EXISTENCE:mutant phenotype:GO:0031593 polyubiquitin modification-dependent protein binding [PMID:32193351]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0045740 positive regulation of DNA replication [PMID:31276951]
Aspartic-type endopeptidase; involved in the cleavage of long-chain polyubiquitinated substrates; role in proteasome-independent, ubiquitin (Ub)-dependent proteolysis and DNA-protein crosslink repair; Ub-shuttle receptor for HO and Ufo1p; DNA damage-inducible v-SNARE binding protein and negative regulator of constitutive exocytosis; possible role in the S-phase checkpoint; UBL-UBA family member containing Ub-like (UBL), helical, Ub-associated (UBA) and retroviral-like proteinase (RVP) domains
putative ubiquitin-specific protease UBP5
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:17145966]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17145966]
Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005381 iron ion transmembrane transporter activity [PMID:16700543|PMID:8599111]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10748025|PMID:16700543]
EXISTENCE:direct assay:GO:0033573 high-affinity iron permease complex [PMID:16522632]
EXISTENCE:genetic interaction:GO:0034755 iron ion transmembrane transport [PMID:8599111]
EXISTENCE:genetic interaction:GO:1901684 arsenate ion transmembrane transport [PMID:23295455]
EXISTENCE:mutant phenotype:GO:0034755 iron ion transmembrane transport [PMID:16700543|PMID:8599111]
High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
hypothetical protein; conserved among S. cerevisiae strains; overlaps verified ORF LSM5/YER146W
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10369684|PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
cohesin-loading factor complex subunit SCC4
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10882066]
EXISTENCE:direct assay:GO:0043515 kinetochore binding [PMID:28938124]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:15610742]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:10882066]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:18708580]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:18708580]
EXISTENCE:mutant phenotype:GO:0071169 establishment of protein localization to chromatin [PMID:10882066]
EXISTENCE:physical interaction:GO:0032116 SMC loading complex [PMID:10882066]
Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
EXISTENCE:curator inference:GO:0000995 RNA polymerase III general transcription initiation factor activity []
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity []
EXISTENCE:direct assay:GO:0000126 transcription factor TFIIIB complex [PMID:1423590|PMID:1458536]
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:2677740]
EXISTENCE:direct assay:GO:0001016 RNA polymerase III transcription regulatory region sequence-specific DNA binding [PMID:8662956]
EXISTENCE:direct assay:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:8895657]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:19098311]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9818719]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:1736286]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
EXISTENCE:direct assay:GO:0070893 transposon integration [PMID:10882723]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:1480467]
EXISTENCE:mutant phenotype:GO:0001188 RNA polymerase I preinitiation complex assembly [PMID:8895657]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:1586947]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12840001]
EXISTENCE:physical interaction:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:8895657]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:8909546]
EXISTENCE:direct assay:GO:0000133 polarisome [PMID:9632790]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:8909546]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:8909546]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:8909546]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:33656555]
EXISTENCE:direct assay:GO:0031489 myosin V binding [PMID:33656555]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:8909546|PMID:19053807]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0007118 budding cell apical bud growth [PMID:10866679]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:8909546]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8013906|PMID:8909546|PMID:33656555]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0031384 regulation of initiation of mating projection growth [PMID:14734532]
EXISTENCE:mutant phenotype:GO:0031385 regulation of termination of mating projection growth [PMID:14734532]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:10085294|PMID:11740491|PMID:8909546]
EXISTENCE:mutant phenotype:GO:2000251 positive regulation of actin cytoskeleton reorganization [PMID:17237521|PMID:19633059]
Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9613572]
EXISTENCE:mutant phenotype:GO:0010447 response to acidic pH [PMID:16980434]
GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication
mRNA-binding ubiquitin-specific protease UBP3
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:1429680]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764|PMID:23222640]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:1429680]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:18498751]
EXISTENCE:genetic interaction:GO:0045053 protein retention in Golgi apparatus [PMID:32673164]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:23476013]
EXISTENCE:mutant phenotype:GO:0016579 protein deubiquitination [PMID:18498751|PMID:23476013|PMID:23645675]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:18391941]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:32673164]
EXISTENCE:mutant phenotype:GO:0047484 regulation of response to osmotic stress [PMID:21743437]
EXISTENCE:mutant phenotype:GO:0060628 regulation of ER to Golgi vesicle-mediated transport [PMID:12778054]
EXISTENCE:physical interaction:GO:0047484 regulation of response to osmotic stress [PMID:21743437]
EXISTENCE:physical interaction:GO:1990861 Ubp3-Bre5 deubiquitination complex [PMID:12778054]
Ubiquitin-specific protease that cleaves Ub-protein fusions; interacts with cofactor Bre5p to regulate transport between ER and Golgi, deubiquitinating COPII and COPI proteins, Sec23p and Sec27p; mediates steady-state retention of Golgi membrane proteins with Bre5p; phosphorylation (S695) by Hog1p regulates transcriptional activation by osmostress; inhibits Ras/PKA signaling through Ira2p; inhibits silencing; role in ribophagy; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0047536 2-aminoadipate transaminase activity [PMID:19342587]
Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12529447|PMID:8389363]
EXISTENCE:mutant phenotype:GO:0003743 translation initiation factor activity [PMID:2833749]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:2833749]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:2833749]
Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
EXISTENCE:direct assay:GO:0030061 mitochondrial crista [PMID:22513091]
EXISTENCE:direct assay:GO:0097002 mitochondrial inner boundary membrane [PMID:22513091]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:14657018]
EXISTENCE:mutant phenotype:GO:0032977 membrane insertase activity [PMID:9285818|PMID:9428747]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:17452441|PMID:9285818|PMID:9428747|PMID:9482871]
EXISTENCE:mutant phenotype:GO:0033615 mitochondrial proton-transporting ATP synthase complex assembly [PMID:17344477]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:22846909]
Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:17914457]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:7962098|PMID:12145202]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17914457]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17914457]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:17914457]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:17914457]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:17914457]
EXISTENCE:genetic interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:17914457]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:12145202]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:7579704]
EXISTENCE:mutant phenotype:GO:0035024 negative regulation of Rho protein signal transduction [PMID:17914457]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:12145202]
Rho GTPase activating protein (RhoGAP); contains RhoGAP activity specific for both Rho1p and Cdc42p, and potentially Rho4p; acts with the RhoGAP Bem3p to negatively regulate Cdc42p-dependent signal transduction; involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence and for the morphogenesis checkpoint in response to actin depolymerization; localizes to sites of polarized growth and the plasma membrane
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:31323700]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:31323700]
EXISTENCE:mutant phenotype:GO:1901857 positive regulation of cellular respiration [PMID:31081026]
Protein involved in mitochondrial function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; human homolog MYG1 is linked to developmental processes and dermatological diseases, complements yeast null mutant
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10512869]
EXISTENCE:mutant phenotype:GO:0000301 retrograde transport, vesicle recycling within Golgi [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0017119 Golgi transport complex [PMID:11703943]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:20065092]
EXISTENCE:mutant phenotype:GO:0140312 cargo adaptor activity [PMID:15229219]
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
hypothetical protein; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
negative cofactor 2 transcription regulator complex subunit BUR6
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8948634|PMID:9023340|PMID:9096360]
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:18703679]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18703679]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:12237409]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9023340]
EXISTENCE:direct assay:GO:0017054 negative cofactor 2 complex [PMID:8948634]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12237409]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10713169]
EXISTENCE:genetic interaction:GO:0003714 transcription corepressor activity [PMID:10713169]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10713169]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:10713169]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18048413]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:18048413]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:9096360]
EXISTENCE:physical interaction:GO:0017054 negative cofactor 2 complex [PMID:9023340]
Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p; bur6 ncb2 double mutation is functionally complemented by coexpression of human DRAP1 and DR1, although the single bur6 mutation is not complemented by its ortholog DRAP1
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
YERCTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
ARS520; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000217 DNA secondary structure binding [PMID:15563464]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:2072912]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2072912|PMID:22932476]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16449659]
EXISTENCE:genetic interaction:GO:0043631 RNA polyadenylation [PMID:16788068]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:17526727]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:16449659|PMID:19057669]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:26109053]
EXISTENCE:mutant phenotype:GO:0140713 histone chaperone activity [PMID:26109053]
Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19889839]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19889839]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:27035942]
EXISTENCE:direct assay:GO:1990165 single-strand break-containing DNA binding [PMID:27035942]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:18936173]
Damaged-DNA binding subunit of Nuclear Excision Repair Factor 2; recognizes and binds damaged DNA with Rad23p during nucleotide excision repair (NER); also involved with Rad23p in turnover of ubiquitylated proteins; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage; RAD4 and RAD51 pathways confer resistance to benzo[a]pyrene dihydrodiol
EXISTENCE:direct assay:GO:0003839 gamma-glutamylcyclotransferase activity [PMID:23070364]
EXISTENCE:direct assay:GO:0006751 glutathione catabolic process [PMID:23070364]
EXISTENCE:mutant phenotype:GO:0006751 glutathione catabolic process [PMID:23070364]
Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:15647753]
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:17259992]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12504018|PMID:12682017]
EXISTENCE:direct assay:GO:0000976 transcription cis-regulatory region binding [PMID:23468649]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:21623345]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:10811623|PMID:33174727]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:10811623]
EXISTENCE:direct assay:GO:0030874 nucleolar chromatin [PMID:17259992]
EXISTENCE:direct assay:GO:0031490 chromatin DNA binding [PMID:12682017]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:15647753]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:15647753]
EXISTENCE:direct assay:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:10811623|PMID:33174727]
EXISTENCE:genetic interaction:GO:0001178 regulation of transcriptional start site selection at RNA polymerase II promoter [PMID:19948887]
EXISTENCE:genetic interaction:GO:0006363 termination of RNA polymerase I transcription [PMID:17259992]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12682017]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:12504018]
EXISTENCE:genetic interaction:GO:2000104 negative regulation of DNA-templated DNA replication [PMID:18245327]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:12504018]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:31222142]
EXISTENCE:mutant phenotype:GO:0034728 nucleosome organization [PMID:10811623]
EXISTENCE:mutant phenotype:GO:1902275 regulation of chromatin organization [PMID:22922743]
EXISTENCE:physical interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12682017]
Chromatin remodeler that regulates various aspects of transcription; acts in conjunction with Isw1b to regulate chromatin structure, maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains chromo domain, helicase domain and DNA-binding domain; component of both SAGA and SLIK complexes; in human, deletion of CHD1 is a frequent event in prostate cancer
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15630021|PMID:15769879|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15630021|PMID:15769879|PMID:11914276]
EXISTENCE:direct assay:GO:0005840 ribosome [PMID:8631901]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:10357826]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:9193001]
EXISTENCE:direct assay:GO:0008428 ribonuclease inhibitor activity [PMID:11889048]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:17908917|PMID:18981231|PMID:19470581|PMID:24291094|P MID:26777405]
EXISTENCE:direct assay:GO:0060211 regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:17766253]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:27184763]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:10357826]
EXISTENCE:mutant phenotype:GO:0008143 poly(A) binding [PMID:9193001]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:9223284]
EXISTENCE:mutant phenotype:GO:0060211 regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:17766253]
EXISTENCE:physical interaction:GO:0034236 protein kinase A catalytic subunit binding [PMID:22899713]
Poly(A) binding protein; interacts with the cleavage factor complex CF I, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G; stimulates, but is not required for the deadenylation activity of the Pan2p-Pan3p poly(A)-ribonuclease complex
aminophospholipid-translocating P4-type ATPase DNF1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12221123|PMID:12631737|PMID:33060204]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0070867 mating projection tip membrane [PMID:25378585]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0006886 intracellular protein transport [PMID:12221123]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:12631737]
EXISTENCE:genetic interaction:GO:0007163 establishment or maintenance of cell polarity [PMID:15461661]
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:12631737|PMID:17015438]
EXISTENCE:genetic interaction:GO:0090555 phosphatidylethanolamine flippase activity [PMID:12631737|PMID:17015438]
EXISTENCE:genetic interaction:GO:0140345 phosphatidylcholine flippase activity [PMID:12631737]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:12631737]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:17015438]
EXISTENCE:mutant phenotype:GO:0090555 phosphatidylethanolamine flippase activity [PMID:17015438|PMID:27432949]
EXISTENCE:mutant phenotype:GO:0099040 ceramide translocation [PMID:33060204]
EXISTENCE:mutant phenotype:GO:0140345 phosphatidylcholine flippase activity [PMID:27432949]
EXISTENCE:mutant phenotype:GO:0140351 glycosylceramide flippase activity [PMID:33060204]
EXISTENCE:physical interaction:GO:1990531 phospholipid-translocating ATPase complex [PMID:15090616]
Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:12518319]
EXISTENCE:mutant phenotype:GO:0010628 positive regulation of gene expression [PMID:19158491]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:7891677]
Serine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations
ARS521; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0001680 tRNA 3'-terminal CCA addition [PMID:1634528]
EXISTENCE:direct assay:GO:0004810 CCA tRNA nucleotidyltransferase [PMID:1634528]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:1634528]
EXISTENCE:direct assay:GO:0052927 CTP:tRNA cytidylyltransferase activity [PMID:23872483]
EXISTENCE:direct assay:GO:0052929 ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity [PMID:23872483]
ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites; human homolog TRNT1 complements yeast null mutant
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10523651]
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10523651]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10523651]
EXISTENCE:direct assay:GO:0032454 histone H3K9 demethylase activity [PMID:17371840]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:21296759|PMID:19111667]
EXISTENCE:direct assay:GO:0051864 histone H3K36 demethylase activity [PMID:17369256|PMID:17371840|PMID:17525156]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:25660547]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:25660547]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:25660547]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10523651]
EXISTENCE:mutant phenotype:GO:0010507 negative regulation of autophagy [PMID:25660547]
EXISTENCE:mutant phenotype:GO:0043565 sequence-specific DNA binding [PMID:21296759]
EXISTENCE:mutant phenotype:GO:0051864 histone H3K36 demethylase activity [PMID:17369256|PMID:17371840|PMID:17525156]
JmjC domain-containing histone demethylase; targets tri- and dimethylated H3K36; associates with actively transcribed regions and promotes elongation; repressor of autophagy-related genes in nutrient-replete conditions; damage-responsive repressor of PHR1; phosphorylated by the Rad53p-dependent DNA damage checkpoint pathway and by a Rim1p-mediated event during starvation; target of stress-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15753074|PMID:8537371]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8537371]
EXISTENCE:direct assay:GO:0009117 nucleotide metabolic process [PMID:15753074]
EXISTENCE:direct assay:GO:0046899 nucleoside triphosphate adenylate kinase activity [PMID:15753074|PMID:8537371]
Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3
EXISTENCE:direct assay:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:8855246]
EXISTENCE:direct assay:GO:0000439 transcription factor TFIIH core complex [PMID:14500720|PMID:19818408]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:8132553]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408|PMID:27381459]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19818408]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:2957691]
EXISTENCE:direct assay:GO:0043139 5'-3' DNA helicase activity [PMID:2827162]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665]
EXISTENCE:mutant phenotype:GO:0000019 regulation of mitotic recombination [PMID:10713167|PMID:9799251]
EXISTENCE:mutant phenotype:GO:0010525 regulation of transposition, RNA-mediated [PMID:10713167]
EXISTENCE:mutant phenotype:GO:0045951 positive regulation of mitotic recombination [PMID:24189665]
5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:8649518]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419|PMID:8670905]
EXISTENCE:mutant phenotype:GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) [PMID:9705931]
EXISTENCE:mutant phenotype:GO:0003724 RNA helicase activity [PMID:9705931|PMID:8649518]
RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:12604797]
EXISTENCE:direct assay:GO:0031389 Rad17 RFC-like complex [PMID:12604797|PMID:10660302]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:9564050]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:11267834]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511543]
EXISTENCE:physical interaction:GO:0031389 Rad17 RFC-like complex [PMID:10660302]
Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:32098918]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14519092]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:17074835]
EXISTENCE:mutant phenotype:GO:0015036 disulfide oxidoreductase activity [PMID:10567543|PMID:27085841]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:20889785]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10567543|PMID:17074835]
EXISTENCE:mutant phenotype:GO:2000678 negative regulation of transcription regulatory region DNA binding [PMID:23045394]
EXISTENCE:physical interaction:GO:0006879 cellular iron ion homeostasis [PMID:17074835]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:23045394]
Glutathione-dependent oxidoreductase and glutathione S-transferase; hydroperoxide/superoxide-radical responsive monothiol glutaredoxin subfamily member with Grx3p and Grx5p; protects cells from oxidative damage with GRX3 and GRX5; with Grx3p, promotes dissociation of Aft1p from iron regulon gene promoters and subsequent nuclear export in iron-replete conditions, regulating iron homeostasis; involved with Grx3p in Sir2p deglutathionylation, restoring deacetylase activity after disulfide stress
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11695919]
EXISTENCE:direct assay:GO:0034198 cellular response to amino acid starvation [PMID:18092814]
EXISTENCE:direct assay:GO:0046547 trans-aconitate 3-methyltransferase activity [PMID:11695919]
Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9434351]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:10836794]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
EXISTENCE:mutant phenotype:GO:0006449 regulation of translational termination [PMID:10836794]
DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:12167636]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12167636|PMID:25409301|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25409301|PMID:22842922]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:24142105]
EXISTENCE:genetic interaction:GO:0001402 signal transduction involved in filamentous growth [PMID:9215628]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:9215628]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:9215628]
EXISTENCE:genetic interaction:GO:0030437 ascospore formation [PMID:9215628]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:24850890]
EXISTENCE:genetic interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:17559233]
EXISTENCE:genetic interaction:GO:0070873 regulation of glycogen metabolic process [PMID:9215628]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:14704161]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:24142105]
EXISTENCE:mutant phenotype:GO:0043066 negative regulation of apoptotic process [PMID:22785534]
EXISTENCE:mutant phenotype:GO:0050815 phosphoserine residue binding [PMID:9822578]
EXISTENCE:mutant phenotype:GO:0070842 aggresome assembly [PMID:23843611]
EXISTENCE:physical interaction:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:17030612]
14-3-3 protein, major isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, the spindle position checkpoint and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; antiapoptotic gene; similar to several human 14-3-3 proteins
pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7589446|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:2007123]
EXISTENCE:direct assay:GO:0006086 acetyl-CoA biosynthetic process from pyruvate [PMID:7947791]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity [PMID:11589696]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
EXISTENCE:direct assay:GO:0000150 DNA strand exchange activity [PMID:23769192]
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:1581960]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:11551925]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:11551925]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141|PMID:7528104]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:30301803]
EXISTENCE:genetic interaction:GO:0007130 synaptonemal complex assembly [PMID:24367271]
EXISTENCE:mutant phenotype:GO:0000709 meiotic joint molecule formation [PMID:22955832|PMID:9323140]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:1581960|PMID:16581767]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:1581960]
Meiosis-specific recombinase required for double-strand break repair; also required for pairing between homologous chromosomes and for the normal morphogenesis of synaptonemal complex; binds ssDNA and dsDNA, forms helical filaments; potent inhibitor of the ATPase activity of Srs2p helicase, blocking its ssDNA translocating motor activity and inhibiting its antirecombinase activity; stimulated by Rdh54p; homolog of Rad51p and the bacterial RecA protein
ARS522; Autonomously Replicating Sequence; active origin of replication near the right end of Chromosome V that is activated late in S phase, late activation appears to result from telomeric proximity
EXISTENCE:direct assay:GO:0004860 protein kinase inhibitor activity [PMID:32423992]
EXISTENCE:direct assay:GO:0051019 mitogen-activated protein kinase binding [PMID:32423992]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:8455558]
Meiosis-specific MAPK inhibitor; binds Smk1p during meiosis I; links APC/C-Ama1 to Smk1p activation; required for sporulation; transcript is induced 7.5 hr after induction of meiosis; Isc10p is ubiquitylated and degraded in an Ama1p-dependent manner upon completion of meiosis II
EXISTENCE:physical interaction:GO:0006886 intracellular protein transport [PMID:12620189]
Protein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
Mitochondrial hypothetical protein; conserved among S. cerevisiae strains; extensively overlaps a Ty1 LTR; YER181C is not an essential gene
EXISTENCE:direct assay:GO:0009396 folic acid-containing compound biosynthetic process [PMID:11923304]
EXISTENCE:direct assay:GO:0030272 5-formyltetrahydrofolate cyclo-ligase activity [PMID:11923304]
5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
EXISTENCE:curator inference:GO:0003700 DNA-binding transcription factor activity [PMID:24998441]
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:24998441]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:24998441|PMID:19111667]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:24998441]
Transcriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:18326586]
EXISTENCE:mutant phenotype:GO:0035351 heme transmembrane transport [PMID:18326586]
Plasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
hypothetical protein; induced in respiratory-deficient cells
hypothetical protein; conserved across S. cerevisiae strains; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p
TEL05R; Telomeric region on the right arm of Chromosome V; annotated components include an X element core sequence, X element combinatorial repeats, and a long Y' element; TEL05R does have telomeric repeats (TEL05R-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
ARS523; Autonomously Replicating Sequence
Y' element ATP-dependent helicase protein 1 copy 2
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9837911]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9837911]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p; induced by treatment with 8-methoxypsoralen and UVA irradiation