Saccharomyces cerevisiae S288C chromosome IV, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (06-FEB-2013) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006938.\n\nOn Apr 26, 2011 this sequence version replaced NC_001136.9.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL04L; Telomeric region on the left arm of Chromosome IV; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats
ARS400; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:mutant phenotype:GO:0038024 cargo receptor activity [PMID:9560393]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005363 maltose transmembrane transporter activity [PMID:12210897]
EXISTENCE:direct assay:GO:0008643 carbohydrate transport [PMID:12210897]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:12210897]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
Alpha-glucoside permease; transports maltose, maltotriose, alpha-methylglucoside, and turanose; almost identical to Mph3p; encoded in a subtelomeric position in a region likely to have undergone duplication
EXISTENCE:mutant phenotype:GO:0003939 L-iditol 2-dehydrogenase activity [PMID:26996892]
Sorbitol dehydrogenase; protein sequence is 99% identical to the Sor1p sorbitol dehydrogenase
EXISTENCE:genetic interaction:GO:0015795 sorbitol transmembrane transport [PMID:26996892]
EXISTENCE:genetic interaction:GO:0015797 mannitol transmembrane transport [PMID:26996892]
EXISTENCE:genetic interaction:GO:1902341 xylitol transmembrane transport [PMID:26996892]
EXISTENCE:mutant phenotype:GO:0005353 fructose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0008645 hexose transmembrane transport [PMID:10618490]
EXISTENCE:mutant phenotype:GO:0015578 mannose transmembrane transporter activity [PMID:10618490]
Putative transmembrane polyol transporter; supports growth on and uptake of mannitol, sorbitol and xylitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose
ARS403; Autonomously Replicating Sequence
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase
EXISTENCE:genetic interaction:GO:0009228 thiamine biosynthetic process [PMID:12777485]
Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
EXISTENCE:direct assay:GO:0047681 aryl-alcohol dehydrogenase (NADP+) activity [PMID:29079624]
Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
hypothetical protein; conserved across S. cerevisiae strains
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDL241W is not an essential gene
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11447600]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:15514049]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11447600]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11447600|PMID:23613772|PMID:14562095]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:23613772]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:15514049]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:15514049]
EXISTENCE:genetic interaction:GO:0000755 cytogamy [PMID:15514049]
EXISTENCE:genetic interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:11447600|PMID:11713681]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:11447600|PMID:11713681]
EXISTENCE:genetic interaction:GO:0090038 negative regulation of protein kinase C signaling [PMID:11713681]
EXISTENCE:genetic interaction:GO:0090334 regulation of cell wall (1->3)-beta-D-glucan biosynthetic process [PMID:11447600]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:15514049]
EXISTENCE:mutant phenotype:GO:0035024 negative regulation of Rho protein signal transduction [PMID:11713681]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:11713681]
EXISTENCE:mutant phenotype:GO:0090038 negative regulation of protein kinase C signaling [PMID:11713681]
GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:11742972|PMID:11973299]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:11742972|PMID:11973299]
EXISTENCE:direct assay:GO:0070056 prospore membrane leading edge [PMID:11742972|PMID:11973299|PMID:24390141]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:17905927]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:17905927]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:11973299]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:11742972]
EXISTENCE:physical interaction:GO:0005816 spindle pole body [PMID:11973299]
Protein required for spore wall formation; subunit of leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms ring-like structure at leading edge of prospore membrane during meiosis II; mediates assembly of LEP complex, formation of ring-like structure via interaction with spindle pole body components, and prospore membrane maturation; also detected in peroxisomes; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006147 guanine catabolic process [PMID:10542258]
EXISTENCE:direct assay:GO:0008892 guanine deaminase activity [PMID:15565584]
Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
hypothetical protein; required for respiratory growth; YDL237W is not an essential gene
EXISTENCE:direct assay:GO:0004035 alkaline phosphatase activity [PMID:9561742]
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:9561742]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0008967 phosphoglycolate phosphatase activity [PMID:27294321]
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:27294321]
EXISTENCE:direct assay:GO:0046838 phosphorylated carbohydrate dephosphorylation [PMID:27294321]
Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14665464]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14665464]
EXISTENCE:direct assay:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:8808622]
EXISTENCE:direct assay:GO:0016772 transferase activity, transferring phosphorus-containing groups [PMID:8808622]
EXISTENCE:mutant phenotype:GO:0007234 osmosensory signaling via phosphorelay pathway [PMID:8808622]
EXISTENCE:physical interaction:GO:0043424 protein histidine kinase binding [PMID:8808622]
Osmotic stress-responsive phosphorelay intermediate sensor protein; phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10508155]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:35134079]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0016192 vesicle-mediated transport [PMID:11118206]
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:10508155]
EXISTENCE:mutant phenotype:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:18809726]
GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking; contains a PH-like domain
Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
olichyl-diphosphooligosaccharide--protein glycotransferase OST4
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26928762]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:12810948]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:10677492]
EXISTENCE:physical interaction:GO:0006487 protein N-linked glycosylation [PMID:9405463]
EXISTENCE:physical interaction:GO:0008250 oligosaccharyltransferase complex [PMID:9405463]
Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11378903]
Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
EXISTENCE:direct assay:GO:0004725 protein tyrosine phosphatase activity [PMID:1649172]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:21460040]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:21460040]
Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:17146552]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19502427]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:1394434|PMID:16413483]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9860955]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9670014]
EXISTENCE:direct assay:GO:0051083 'de novo' cotranslational protein folding [PMID:9670014]
EXISTENCE:genetic interaction:GO:0000054 ribosomal subunit export from nucleus [PMID:20368619]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:20368619]
EXISTENCE:genetic interaction:GO:0042149 cellular response to glucose starvation [PMID:19723765]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:1394434]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:17483428]
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:15456889]
EXISTENCE:mutant phenotype:GO:0006452 translational frameshifting [PMID:16607023]
EXISTENCE:physical interaction:GO:0002181 cytoplasmic translation [PMID:1394434]
Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
ARS404; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:6313222|PMID:6099261]
EXISTENCE:direct assay:GO:0007534 gene conversion at mating-type locus [PMID:6313222]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:6297747]
Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p
EXISTENCE:direct assay:GO:0003779 actin binding [PMID:10069805]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:15975906|PMID:8816753]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:16452633]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16452633]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9927415]
EXISTENCE:mutant phenotype:GO:0005856 cytoskeleton [PMID:10069805]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9927415]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:9927415]
EXISTENCE:mutant phenotype:GO:0030037 actin filament reorganization involved in cell cycle [PMID:10069805]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:16926193]
ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:17603111]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:22144691|PMID:29321251]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:14597621]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0061163 endoplasmic reticulum polarization [PMID:25083872]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:18431499]
EXISTENCE:genetic interaction:GO:0000921 septin ring assembly [PMID:17603111]
EXISTENCE:genetic interaction:GO:0097271 protein localization to bud neck [PMID:25971666]
EXISTENCE:mutant phenotype:GO:0000917 division septum assembly [PMID:17603111]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:22144691]
EXISTENCE:mutant phenotype:GO:0010458 exit from mitosis [PMID:12699621]
EXISTENCE:mutant phenotype:GO:0032186 cellular bud neck septin ring organization [PMID:12636916]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:17603111]
EXISTENCE:physical interaction:GO:0005935 cellular bud neck [PMID:25083872]
Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
EXISTENCE:genetic interaction:GO:0008361 regulation of cell size [PMID:11290704]
EXISTENCE:genetic interaction:GO:0061157 mRNA destabilization [PMID:24386402]
Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:11923536]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:11923536]
Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11784867]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:11784867]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:11784867]
Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:9710643]
EXISTENCE:direct assay:GO:0010521 telomerase inhibitor activity [PMID:19043074]
EXISTENCE:direct assay:GO:0032210 regulation of telomere maintenance via telomerase [PMID:27659693]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17166094|PMID:8824190|PMID:8943008]
EXISTENCE:direct assay:GO:0051974 negative regulation of telomerase activity [PMID:19043074]
EXISTENCE:direct assay:GO:0061770 translation elongation factor binding [PMID:31006804]
EXISTENCE:genetic interaction:GO:0007004 telomere maintenance via telomerase [PMID:11239396]
EXISTENCE:genetic interaction:GO:0032210 regulation of telomere maintenance via telomerase [PMID:11046137]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:11390652]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:8978029]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:7565765]
EXISTENCE:mutant phenotype:GO:0043047 single-stranded telomeric DNA binding [PMID:17166094]
EXISTENCE:mutant phenotype:GO:0071166 ribonucleoprotein complex localization [PMID:25387524]
EXISTENCE:physical interaction:GO:1990879 CST complex [PMID:11230140]
Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle
EXISTENCE:mutant phenotype:GO:0006399 tRNA metabolic process [PMID:10918062]
EXISTENCE:mutant phenotype:GO:0051500 D-tyrosyl-tRNA(Tyr) deacylase activity [PMID:10766779]
EXISTENCE:mutant phenotype:GO:0097358 D-leucyl-tRNA(Leu) deacylase activity [PMID:10918062]
EXISTENCE:mutant phenotype:GO:1900829 D-tyrosine catabolic process [PMID:10918062]
EXISTENCE:mutant phenotype:GO:1900832 D-leucine catabolic process [PMID:10918062]
D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
hypothetical protein; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:32591483]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:32591483]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:11864609]
EXISTENCE:direct assay:GO:0030943 mitochondrion targeting sequence binding [PMID:11864609]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:10648604|PMID:10637294]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:32591483]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:32591483]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:8955274|PMID:32591483|PMID:10648604]
Essential core component of mitochondrial TIM22 complex; involved in insertion of polytopic proteins into inner membrane; forms channel through which proteins are imported; different regions of Tim22p have functionally diverse roles in recruiting partner subunits to core channel
EXISTENCE:direct assay:GO:0008180 COP9 signalosome [PMID:12446563]
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:12186635|PMID:12446563|PMID:24164706]
EXISTENCE:mutant phenotype:GO:0000754 adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion [PMID:12446563]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:12183637]
EXISTENCE:mutant phenotype:GO:0070452 positive regulation of ergosterol biosynthetic process [PMID:24164706]
Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis
EXISTENCE:direct assay:GO:0004352 glutamate dehydrogenase (NAD+) activity [PMID:6343120]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:6343120]
EXISTENCE:direct assay:GO:0006807 nitrogen compound metabolic process [PMID:6343120]
EXISTENCE:mutant phenotype:GO:0004352 glutamate dehydrogenase (NAD+) activity [PMID:1975578]
EXISTENCE:mutant phenotype:GO:0006807 nitrogen compound metabolic process [PMID:1975578]
NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11337509]
EXISTENCE:genetic interaction:GO:0031138 negative regulation of conjugation with cellular fusion [PMID:11337509]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11337509]
EXISTENCE:mutant phenotype:GO:0031138 negative regulation of conjugation with cellular fusion [PMID:11337509]
Serine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:17425675|PMID:14562095]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:21282979]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:21893585]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:22588997|PMID:21282979]
EXISTENCE:genetic interaction:GO:0042274 ribosomal small subunit biogenesis [PMID:17425675]
rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:1423607|PMID:15623581]
EXISTENCE:genetic interaction:GO:0090114 COPII-coated vesicle budding [PMID:10564255]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15623581]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:10564255|PMID:15623581]
Endoplasmic reticulum packaging chaperone; required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14985124|PMID:26928762]
EXISTENCE:genetic interaction:GO:0015495 gamma-aminobutyric acid:proton symporter activity [PMID:8455553]
EXISTENCE:genetic interaction:GO:0015812 gamma-aminobutyric acid transport [PMID:8455553]
EXISTENCE:genetic interaction:GO:0055085 transmembrane transport [PMID:8455553]
EXISTENCE:mutant phenotype:GO:0015489 putrescine transmembrane transporter activity [PMID:14985124]
EXISTENCE:mutant phenotype:GO:0015812 gamma-aminobutyric acid transport [PMID:14985124]
EXISTENCE:mutant phenotype:GO:0015847 putrescine transport [PMID:14985124]
GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
ARS405; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:19435883]
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:19435883]
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:19435883]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:19435883]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:19435883]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:12088152]
EXISTENCE:physical interaction:GO:0000974 Prp19 complex [PMID:12088152]
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11433018|PMID:9843512]
EXISTENCE:direct assay:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:direct assay:GO:0034513 box H/ACA snoRNA binding [PMID:11433018]
EXISTENCE:mutant phenotype:GO:0000469 cleavage involved in rRNA processing [PMID:9843512]
EXISTENCE:mutant phenotype:GO:0000493 box H/ACA snoRNP assembly [PMID:11433018]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9843512]
EXISTENCE:mutant phenotype:GO:0031120 snRNA pseudouridine synthesis [PMID:15962000]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:9843512]
Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
EXISTENCE:direct assay:GO:0000822 inositol hexakisphosphate binding [PMID:16783363]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:15459192]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10610322]
EXISTENCE:direct assay:GO:0006409 tRNA export from nucleus [PMID:31453808]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:16783364]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:10610322]
EXISTENCE:genetic interaction:GO:0008047 enzyme activator activity [PMID:16783364]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:20371601]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:18724935]
EXISTENCE:mutant phenotype:GO:0006449 regulation of translational termination [PMID:20371601]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:16783364]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:8898365|PMID:27385342]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:18724935]
Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
hypothetical protein; YDL206W is not an essential protein
EXISTENCE:mutant phenotype:GO:0004418 hydroxymethylbilane synthase activity [PMID:1508149|PMID:323256]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:14559249|PMID:323256]
Porphobilinogen deaminase; catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme; human homolog HMBS can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:17905927]
EXISTENCE:direct assay:GO:0071782 endoplasmic reticulum tubular network [PMID:16469703]
EXISTENCE:genetic interaction:GO:0032581 ER-dependent peroxisome organization [PMID:27129769]
EXISTENCE:genetic interaction:GO:0034976 response to endoplasmic reticulum stress [PMID:27117666]
EXISTENCE:genetic interaction:GO:0048309 endoplasmic reticulum inheritance [PMID:27117666]
EXISTENCE:genetic interaction:GO:0071786 endoplasmic reticulum tubular network organization [PMID:16469703|PMID:18309084|PMID:18442980]
EXISTENCE:genetic interaction:GO:0071788 endoplasmic reticulum tubular network maintenance [PMID:21502358]
Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress
EXISTENCE:mutant phenotype:GO:0009967 positive regulation of signal transduction [PMID:18806213]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:18806213]
Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
mitochondrial 54S ribosomal protein YmL11
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:9151978]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:9151978]
Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
EXISTENCE:direct assay:GO:0008176 tRNA (guanine-N7-)-methyltransferase activity [PMID:17382321]
EXISTENCE:direct assay:GO:0106004 tRNA (guanine-N7)-methylation [PMID:17382321]
EXISTENCE:direct assay:GO:0106143 tRNA (m7G46) methyltransferase complex [PMID:12403464|PMID:17382321|PMID:18184583]
EXISTENCE:mutant phenotype:GO:0008176 tRNA (guanine-N7-)-methyltransferase activity [PMID:12403464]
EXISTENCE:mutant phenotype:GO:0106004 tRNA (guanine-N7)-methylation [PMID:12403464]
Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
methylated-DNA--protein-cysteine methyltransferase
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:1641326]
EXISTENCE:direct assay:GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity [PMID:2403555|PMID:2065659]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
EXISTENCE:mutant phenotype:GO:0006307 DNA dealkylation involved in DNA repair [PMID:1641326]
DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage; localizes to the peroxisome in a Pex5p-dependent manner
Putative transporter; member of the sugar porter family
EXISTENCE:direct assay:GO:0001408 guanine nucleotide transport [PMID:14998997]
EXISTENCE:direct assay:GO:0001409 guanine nucleotide transmembrane transporter activity [PMID:14998997]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:14998997]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:14998997]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:14629196]
Mitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:15282802]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:9290207]
Anti-silencing protein; causes derepression of silent loci when overexpressed
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:17604721]
EXISTENCE:direct assay:GO:0030127 COPII vesicle coat [PMID:8004676]
EXISTENCE:direct assay:GO:0090114 COPII-coated vesicle budding [PMID:8223424]
EXISTENCE:mutant phenotype:GO:0090114 COPII-coated vesicle budding [PMID:12475940]
Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2406560|PMID:24124599]
EXISTENCE:genetic interaction:GO:0051594 detection of glucose [PMID:8901598]
EXISTENCE:mutant phenotype:GO:0010255 glucose mediated signaling pathway [PMID:9564039]
EXISTENCE:mutant phenotype:GO:0015755 fructose transmembrane transport [PMID:2046678]
EXISTENCE:mutant phenotype:GO:0015761 mannose transmembrane transport [PMID:2046678]
EXISTENCE:mutant phenotype:GO:0045835 negative regulation of meiotic nuclear division [PMID:18616605]
EXISTENCE:mutant phenotype:GO:0051594 detection of glucose [PMID:8901598]
EXISTENCE:mutant phenotype:GO:1904659 glucose transmembrane transport [PMID:2046678]
Plasma membrane low glucose sensor, regulates glucose transport; high affinity sensor that contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication
ditrans,polycis-polyprenyl diphosphate synthase
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:14690591]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11086160]
EXISTENCE:direct assay:GO:0019408 dolichol biosynthetic process [PMID:25066056]
EXISTENCE:direct assay:GO:0045547 dehydrodolichyl diphosphate synthase activity [PMID:25066056]
EXISTENCE:direct assay:GO:1904423 dehydrodolichyl diphosphate synthase complex [PMID:25066056]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:16943325]
Forms dehydrodolichyl diphosphate synthase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:8816753]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:20841378|PMID:14562095]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9736713|PMID:9813082]
EXISTENCE:direct assay:GO:1903292 protein localization to Golgi membrane [PMID:33788598]
EXISTENCE:direct assay:GO:1903358 regulation of Golgi organization [PMID:33788598]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20444982]
EXISTENCE:physical interaction:GO:0006893 Golgi to plasma membrane transport [PMID:16498409]
ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; involved in mitochondrial morphology; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:20392820]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:20392820]
Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15652483|PMID:14562095]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:10089879]
EXISTENCE:direct assay:GO:0034450 ubiquitin-ubiquitin ligase activity [PMID:10089879]
EXISTENCE:direct assay:GO:0070936 protein K48-linked ubiquitination [PMID:28165462]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:7615550|PMID:10089879]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:21148305]
EXISTENCE:mutant phenotype:GO:0031398 positive regulation of protein ubiquitination [PMID:27226596]
Ubiquitin (Ub) chain assembly factor (E4); forms branched Ub chains on substrates destined for degradation, catalyzing K48-linked multi-monoubiquitination of K29-linked Ub chains assembled by the Ub-ligase Ufd4p; involved in the Ub-fusion degradation pathway, OLE membrane fluidity pathway, stress response survival and the ERAD pathway for misfolded proteins, where it promotes ubiquitination and transfer of ERAD substrates to Rad23p; U-box family member; homologous to human Ufd2a and Ufd2b
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25605335]
EXISTENCE:genetic interaction:GO:0006012 galactose metabolic process [PMID:12372297]
EXISTENCE:mutant phenotype:GO:1990115 RNA polymerase III assembly [PMID:25605335]
Protein involved in assembly of the RNA Pol III complex; high copy suppressor of Pol III assembly mutation and psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; physically interacts with Pol III; proposed to bind single-stranded nucleic acids via its R3H domain; capable of forming the prion [VTS1+]
ARS406; Autonomously Replicating Sequence
phosphoprotein phosphatase 2A catalytic subunit PPH22
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:16541024]
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:12135475|PMID:25387524]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:12518319]
EXISTENCE:genetic interaction:GO:1903432 regulation of TORC1 signaling [PMID:12820961]
EXISTENCE:genetic interaction:GO:2000786 positive regulation of autophagosome assembly [PMID:27973551]
EXISTENCE:mutant phenotype:GO:2000198 negative regulation of ribonucleoprotein complex localization [PMID:25387524]
EXISTENCE:physical interaction:GO:0006417 regulation of translation [PMID:10329624]
Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph21p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
hypothetical protein; YDL186W is not an essential gene
H(+)-transporting V1 sector ATPase subunit A
EXISTENCE:direct assay:GO:0000221 vacuolar proton-transporting V-type ATPase, V1 domain [PMID:1530931|PMID:2478556|PMID:8626613]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:7775427|PMID:26928762]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0019538 protein metabolic process [PMID:8508780]
EXISTENCE:direct assay:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:8718887]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:29519938|PMID:18048916]
Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
EXISTENCE:direct assay:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:18782943]
Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:20197279]
EXISTENCE:genetic interaction:GO:0006813 potassium ion transport [PMID:20197279]
EXISTENCE:genetic interaction:GO:1902600 proton transmembrane transport [PMID:20197279]
Protein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system
EXISTENCE:direct assay:GO:0004410 homocitrate synthase activity [PMID:14984204]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9099739|PMID:11914276]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:25628362]
EXISTENCE:genetic interaction:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:18524920]
EXISTENCE:mutant phenotype:GO:0004410 homocitrate synthase activity [PMID:8923736]
Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:12167646]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12809520|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0032780 negative regulation of ATP-dependent activity [PMID:12809520]
EXISTENCE:direct assay:GO:0042030 ATPase inhibitor activity [PMID:15294286]
Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:36047762|PMID:26928762]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:36047762]
G protein-coupled receptor-like protein; involved in negatively regulating TORC1 activity; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:17853895]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17853895]
EXISTENCE:genetic interaction:GO:0032878 regulation of establishment or maintenance of cell polarity [PMID:17853895]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:9593297]
EXISTENCE:physical interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:9593297]
EXISTENCE:physical interaction:GO:0032878 regulation of establishment or maintenance of cell polarity [PMID:17853895]
Cyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004458 D-lactate dehydrogenase (cytochrome) activity [PMID:10509019]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10509019]
EXISTENCE:direct assay:GO:0051990 (R)-2-hydroxyglutarate dehydrogenase activity [PMID:26774271]
EXISTENCE:direct assay:GO:0071949 FAD binding [PMID:26774271]
EXISTENCE:mutant phenotype:GO:0003779 actin binding [PMID:15158445]
EXISTENCE:mutant phenotype:GO:1903457 lactate catabolic process [PMID:26774271]
D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase; mitochondrial matrix protein that oxidizes D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate with a minor role in lactate catabolism; located in the mitochondrial matrix
hypothetical protein; similar to the mouse IMPACT gene; YDL177C is not an essential gene
EXISTENCE:direct assay:GO:0043254 regulation of protein-containing complex assembly [PMID:35650207]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:32064787]
hypothetical protein involved in regulating assembly of Gid4p into the GID E3 ligase complex; interacts with GID complex components; involved in actin patch formation; computationally predicted to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; non-essential gene
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:22402490]
EXISTENCE:direct assay:GO:0004652 polynucleotide adenylyltransferase activity [PMID:15935759|PMID:15935758]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16541108]
EXISTENCE:direct assay:GO:0031499 TRAMP complex [PMID:15828860|PMID:15935758|PMID:15935759]
EXISTENCE:direct assay:GO:0034458 3'-5' RNA helicase activity [PMID:22532666]
EXISTENCE:direct assay:GO:0043629 ncRNA polyadenylation [PMID:15828860|PMID:15935758]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:15935758|PMID:17643380]
EXISTENCE:genetic interaction:GO:0004652 polynucleotide adenylyltransferase activity [PMID:15935759]
EXISTENCE:genetic interaction:GO:0071031 nuclear mRNA surveillance of mRNA 3'-end processing [PMID:17410208]
EXISTENCE:genetic interaction:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:15935758|PMID:18007593]
EXISTENCE:genetic interaction:GO:0071036 nuclear polyadenylation-dependent snoRNA catabolic process [PMID:15935758]
EXISTENCE:genetic interaction:GO:0071037 nuclear polyadenylation-dependent snRNA catabolic process [PMID:15935758]
EXISTENCE:genetic interaction:GO:0071039 nuclear polyadenylation-dependent CUT catabolic process [PMID:18591258]
EXISTENCE:mutant phenotype:GO:0006400 tRNA modification [PMID:22319136]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:22402490]
RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9525904]
EXISTENCE:mutant phenotype:GO:0004458 D-lactate dehydrogenase (cytochrome) activity [PMID:8492799]
EXISTENCE:mutant phenotype:GO:1903457 lactate catabolic process [PMID:26774271|PMID:8492799]
Major mitochondrial D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26172854|PMID:14562095]
Low complexity protein; mediates inhibition of Mep1p and Mep3p activity; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylation and localization regulated by TORC1-effector kinase, Npr1p
EXISTENCE:direct assay:GO:0016040 glutamate synthase (NADH) activity [PMID:7836314]
EXISTENCE:expression pattern:GO:0006537 glutamate biosynthetic process [PMID:9657994]
EXISTENCE:expression pattern:GO:0019676 ammonia assimilation cycle [PMID:11356843]
EXISTENCE:mutant phenotype:GO:0016040 glutamate synthase (NADH) activity [PMID:7836314]
NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12235130]
EXISTENCE:direct assay:GO:0001010 RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity [PMID:21515579]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:21515579]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19159085]
EXISTENCE:genetic interaction:GO:0001010 RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity [PMID:21515579]
EXISTENCE:mutant phenotype:GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter [PMID:7899074]
EXISTENCE:mutant phenotype:GO:0009450 gamma-aminobutyric acid catabolic process [PMID:3888627]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:7899074]
Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4
hypothetical protein; transcript accumulates in response to any combination of stress conditions
bifunctional alcohol dehydrogenase/S-(hydroxymethyl)glutathione dehydrogenase
EXISTENCE:direct assay:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:8483449]
EXISTENCE:direct assay:GO:0046294 formaldehyde catabolic process [PMID:8483449]
EXISTENCE:direct assay:GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity [PMID:8483449]
EXISTENCE:genetic interaction:GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0004022 alcohol dehydrogenase (NAD+) activity [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0033833 hydroxymethylfurfural reductase (NADH) activity [PMID:16652391]
EXISTENCE:mutant phenotype:GO:0033859 furaldehyde metabolic process [PMID:16652391]
Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:26777405]
Putative RNA binding hypothetical protein; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
EXISTENCE:direct assay:GO:0004017 adenylate kinase activity [PMID:24823650]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10692169]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:24823650]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16287850|PMID:12837249]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:16287850]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10692169]
Essential NTPase required for small ribosome subunit synthesis; mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p; complex with Rps14 is conserved between humans, yeast, and arches; may be the endonuclease for site D; depletion leads to accumulation of pre-40S ribosomes in 80S-like ribosomes; human TAF9 functionally complements the lethality of the null mutation
EXISTENCE:direct assay:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11889048]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7926748]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:26952104]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:2111445]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16926149]
EXISTENCE:mutant phenotype:GO:0031087 deadenylation-independent decapping of nuclear-transcribed mRNA [PMID:15706350]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:2111445]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:10490603]
Component of the CCR4-NOT core complex, involved in mRNA decapping; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor
EXISTENCE:direct assay:GO:0003910 DNA ligase (ATP) activity [PMID:1445910|PMID:6347688]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10531002]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10531002|PMID:24769239]
EXISTENCE:direct assay:GO:0006266 DNA ligation [PMID:9759502]
EXISTENCE:direct assay:GO:0006273 lagging strand elongation [PMID:9759502]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:6749599]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:10531002]
EXISTENCE:mutant phenotype:GO:0006266 DNA ligation [PMID:9759502]
EXISTENCE:mutant phenotype:GO:0006273 lagging strand elongation [PMID:9759502]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:10052932]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:10052932]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:9230305]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:16079237]
DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:10449404]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:10449404]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:12529323]
EXISTENCE:direct assay:GO:0070530 K63-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:10449404]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:31370222]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10449404]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:10449404]
Epsin-like protein involved in endocytosis and actin patch assembly; K63-specific Ub chain binding protein that functions as an endocytic adaptor; binds clathrin via a clathrin-binding domain motif at C-terminus; contains two ubiquitin-interacting motifs (UIMs); functionally redundant with Ent2p; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0071821 FANCM-MHF complex [PMID:22325783]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:20347428]
Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2
DExD/H-box ATP-dependent RNA helicase DHH1
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922|PMID:12032091]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:20368989|PMID:26777405]
EXISTENCE:direct assay:GO:0010603 regulation of cytoplasmic mRNA processing body assembly [PMID:27692063]
EXISTENCE:direct assay:GO:0098562 cytoplasmic side of membrane [PMID:27146487]
EXISTENCE:genetic interaction:GO:0033962 P-body assembly [PMID:18981231]
EXISTENCE:genetic interaction:GO:0045900 negative regulation of translational elongation [PMID:22719226]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:11780629]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:32989641]
EXISTENCE:mutant phenotype:GO:0010603 regulation of cytoplasmic mRNA processing body assembly [PMID:27692063]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:32989641]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:21844211]
EXISTENCE:mutant phenotype:GO:0033962 P-body assembly [PMID:18182159]
EXISTENCE:mutant phenotype:GO:0034063 stress granule assembly [PMID:18981231|PMID:20368989]
EXISTENCE:mutant phenotype:GO:0036267 invasive filamentous growth [PMID:32989641]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:17041186|PMID:18182159|PMID:30973873]
EXISTENCE:mutant phenotype:GO:0045900 negative regulation of translational elongation [PMID:22719226]
Cytoplasmic DEAD-box helicase and mRNA decapping activator; interacts with decapping and deadenylase complexes to coordinate mRNA decapping and decay; regulates general translational repression; translational activator of select mRNAs during filamentous growth, mating and autophagy; cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body assembly, while ATPase stimulation by Not1p promotes disassembly; forms cytoplasmic foci on replication stress
hypothetical protein; identified by sequence comparison with hemiascomycetous yeast species
mitogen-activated protein kinase kinase STE7
EXISTENCE:direct assay:GO:0004708 MAP kinase kinase activity [PMID:8668180|PMID:8384702]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11781566]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:11781566|PMID:17952059]
EXISTENCE:direct assay:GO:0071507 pheromone response MAPK cascade [PMID:8159759|PMID:8455599|PMID:8384702]
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:10880465]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:9363895]
EXISTENCE:genetic interaction:GO:0071507 pheromone response MAPK cascade [PMID:8455599]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:15456892]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:8001818]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8643578]
EXISTENCE:mutant phenotype:GO:0010525 regulation of transposition, RNA-mediated [PMID:9566871]
EXISTENCE:mutant phenotype:GO:0071507 pheromone response MAPK cascade [PMID:8159759|PMID:8384702]
EXISTENCE:physical interaction:GO:0071507 pheromone response MAPK cascade [PMID:8668180]
Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
hypothetical protein; localizes to the ER and peroxisomes; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:22367945]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:22367945]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22367945|PMID:14562095|PMID:22842922]
EXISTENCE:mutant phenotype:GO:2000001 regulation of DNA damage checkpoint [PMID:25817432]
Nuclear protein with role in transcription, protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; also localizes to coding regions of transcribed genes; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12972503|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12972503|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:32553169]
EXISTENCE:direct assay:GO:0007135 meiosis II [PMID:18423199]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:11438663|PMID:1387566|PMID:16688214]
EXISTENCE:genetic interaction:GO:1901673 regulation of mitotic spindle assembly [PMID:8319908]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:8388545|PMID:8455600]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:1427070]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:8388545|PMID:8455600]
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:8001134]
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:30467025]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0062037 D-loop DNA binding [PMID:30467025]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:8001134|PMID:15066280|PMID:9374523]
EXISTENCE:physical interaction:GO:0062128 MutSgamma complex [PMID:9374523]
Protein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9611201]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309|PMID:12837249]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292]
Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene
DNA-directed RNA polymerase III subunit C53
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:1406624]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:16874040|PMID:18497569|PMID:23129774]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16874040]
EXISTENCE:direct assay:GO:0030659 cytoplasmic vesicle membrane [PMID:23129774]
EXISTENCE:direct assay:GO:0061908 phagophore [PMID:23549786]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:10662773|PMID:23129774|PMID:24881874]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:10662773]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:23129774]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
Lipid scramblase that mediates autophagosomal membrane expansion; translocates phospholipids between outer and inner leaflets of liposomes; transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between phagophore assembly site (PAS) and other cytosolic punctate structures
snoRNA-binding rRNA-processing protein NOP14
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10684247|PMID:12068309|PMID:12150911]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:15167894]
EXISTENCE:direct assay:GO:0034511 U3 snoRNA binding [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11694595]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:11694595]
EXISTENCE:physical interaction:GO:0030692 Noc4p-Nop14p complex [PMID:12446671]
Nucleolar protein; forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
proteasome regulatory particle lid subunit RPN5
EXISTENCE:direct assay:GO:0000502 proteasome complex [PMID:21289098]
EXISTENCE:direct assay:GO:0008180 COP9 signalosome [PMID:21289098]
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:direct assay:GO:0034515 proteasome storage granule [PMID:18504300]
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:21289098]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:27226596]
Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:19506040]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:19506040]
Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
EXISTENCE:direct assay:GO:0030126 COPI vesicle coat [PMID:21435344]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9427388]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8001155]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:27068463]
EXISTENCE:mutant phenotype:GO:0030126 COPI vesicle coat [PMID:8001155]
Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
chaperonin-containing T-complex subunit CCT4
EXISTENCE:direct assay:GO:0005832 chaperonin-containing T-complex [PMID:16762366]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:16762366]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16762366]
EXISTENCE:physical interaction:GO:0005832 chaperonin-containing T-complex [PMID:15704212]
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0008610 lipid biosynthetic process [PMID:7564918]
EXISTENCE:direct assay:GO:0008808 cardiolipin synthase activity [PMID:7564918]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:25843549]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:26235513]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:27402848]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:18799619]
EXISTENCE:mutant phenotype:GO:0007006 mitochondrial membrane organization [PMID:10777514]
EXISTENCE:mutant phenotype:GO:0008610 lipid biosynthetic process [PMID:9614098]
EXISTENCE:mutant phenotype:GO:0008808 cardiolipin synthase activity [PMID:9614098]
Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
biotin--[acetyl-CoA-carboxylase] ligase BPL1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0036211 protein modification process [PMID:10551847]
EXISTENCE:direct assay:GO:0071734 biotin-[pyruvate-carboxylase] ligase activity [PMID:10551847]
EXISTENCE:mutant phenotype:GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity [PMID:6108218]
EXISTENCE:mutant phenotype:GO:0036211 protein modification process [PMID:6108218]
Biotin:apoprotein ligase; covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon; human homolog HLCS can complement yeast BPL1 mutant
DNA-directed RNA polymerase II core subunit RPO21
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:26945063]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:3299050]
EXISTENCE:mutant phenotype:GO:0019985 translesion synthesis [PMID:22405652]
RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
EXISTENCE:direct assay:GO:0000779 condensed chromosome, centromeric region [PMID:17569568|PMID:17574026]
EXISTENCE:direct assay:GO:2000059 negative regulation of ubiquitin-dependent protein catabolic process [PMID:21070970]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:17548816]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:17569568|PMID:17574026]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:17569568]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:17548816|PMID:17569568]
EXISTENCE:mutant phenotype:GO:0097030 CENP-A containing nucleosome binding [PMID:21412236]
EXISTENCE:physical interaction:GO:0000779 condensed chromosome, centromeric region [PMID:17548816]
EXISTENCE:physical interaction:GO:0097030 CENP-A containing nucleosome binding [PMID:21412236]
Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitination; homolog of mammalian HJURP
ARS409; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0005536 glucose binding [PMID:29954842]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:8901598]
Plasma membrane high glucose sensor that regulates glucose transport; low affinity sesnor that contains 12 predicted transmembrane segments and a long C-terminal tail required for hexose transporter induction; phosphorylation of the tail by Yck1p/Yck2p facilitates binding to the HXT co-repressors, Mth1p and Std1p; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005094 Rho GDP-dissociation inhibitor activity [PMID:10066831]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10066831|PMID:26928762]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:15108020]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15108020]
EXISTENCE:direct assay:GO:0007015 actin filament organization [PMID:9242378]
EXISTENCE:direct assay:GO:0032889 regulation of vacuole fusion, non-autophagic [PMID:11598008]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:9242378]
EXISTENCE:physical interaction:GO:0007266 Rho protein signal transduction [PMID:9242378]
Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
phosphoprotein phosphatase 2A catalytic subunit PPH21
EXISTENCE:direct assay:GO:0000159 protein phosphatase type 2A complex [PMID:17550305]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:2176150]
Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph22p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0043085 positive regulation of catalytic activity [PMID:21347278]
Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16421250]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:9346239|PMID:10385629]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:19882662|PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:9346238]
EXISTENCE:genetic interaction:GO:0000086 G2/M transition of mitotic cell cycle [PMID:9736614|PMID:7954792]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8943317]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:9499404]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238|PMID:9346239]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:9499404]
Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant
EXISTENCE:direct assay:GO:0004410 homocitrate synthase activity [PMID:10103047]
EXISTENCE:mutant phenotype:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:18524920]
Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12167646|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0051117 ATPase binding [PMID:2172220]
Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:22096102]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:22096102]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:1601875]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:1601875]
Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276|PMID:22842922]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:8628289]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20709757|PMID:26928762]
EXISTENCE:direct assay:GO:0006816 calcium ion transport [PMID:8628289]
EXISTENCE:direct assay:GO:0015369 calcium:proton antiporter activity [PMID:8628289]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:8628289]
EXISTENCE:mutant phenotype:GO:0006816 calcium ion transport [PMID:8628289]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:8628289]
EXISTENCE:mutant phenotype:GO:0015369 calcium:proton antiporter activity [PMID:20709757|PMID:8628289]
EXISTENCE:mutant phenotype:GO:0015386 potassium:proton antiporter activity [PMID:20709757]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:8628289]
Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
EXISTENCE:direct assay:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:7973731]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:14688790]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14688790]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:14688790]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14688790]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:7973731]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:19458192]
EXISTENCE:physical interaction:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:7973731]
Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication
ARS409.5; Strong autonomously replicating sequence
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9436996|PMID:26928762]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:21070972|PMID:24769239]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15910746]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15910746|PMID:26928762]
EXISTENCE:direct assay:GO:0006274 DNA replication termination [PMID:31509741]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:7492595]
EXISTENCE:direct assay:GO:0030894 replisome [PMID:31509741]
EXISTENCE:direct assay:GO:0030970 retrograde protein transport, ER to cytosol [PMID:32588820]
EXISTENCE:direct assay:GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex [PMID:11598205|PMID:11733065]
EXISTENCE:direct assay:GO:0036266 Cdc48p-Npl4p-Vms1p AAA ATPase complex [PMID:21070972]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:11733065]
EXISTENCE:direct assay:GO:1990112 RQC complex [PMID:23178123]
EXISTENCE:genetic interaction:GO:0032984 protein-containing complex disassembly [PMID:26255844]
EXISTENCE:genetic interaction:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0010636 positive regulation of mitochondrial fusion [PMID:29309037]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:20855502]
EXISTENCE:mutant phenotype:GO:0016320 endoplasmic reticulum membrane fusion [PMID:7553849]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:23418575]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11739805|PMID:11756557]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:11740563|PMID:32588820]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:20483956]
EXISTENCE:mutant phenotype:GO:0032984 protein-containing complex disassembly [PMID:25342810|PMID:26255844]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:20508643]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:20855502]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:20206597|PMID:29309037]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0051228 mitotic spindle disassembly [PMID:14636562]
EXISTENCE:mutant phenotype:GO:0051974 negative regulation of telomerase activity [PMID:26365526]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:22505030]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:18812321]
EXISTENCE:mutant phenotype:GO:0071630 nuclear protein quality control by the ubiquitin-proteasome system [PMID:22718752]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:11740563|PMID:11813000]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579|PMID:21070972]
EXISTENCE:mutant phenotype:GO:0099638 endosome to plasma membrane protein transport [PMID:36125415]
EXISTENCE:mutant phenotype:GO:0120174 stress-induced homeostatically regulated protein degradation pathway [PMID:29861160]
EXISTENCE:mutant phenotype:GO:1900182 positive regulation of protein localization to nucleus [PMID:11598205|PMID:11733065]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23358411|PMID:23479637]
EXISTENCE:mutant phenotype:GO:1990171 SCF complex disassembly in response to cadmium stress [PMID:23000173]
EXISTENCE:mutant phenotype:GO:2001168 positive regulation of histone H2B ubiquitination [PMID:25183520]
EXISTENCE:physical interaction:GO:1990112 RQC complex [PMID:23479637]
AAA ATPase with protein-unfoldase activity; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:direct assay:GO:0009117 nucleotide metabolic process [PMID:11805111]
EXISTENCE:direct assay:GO:0043530 adenosine 5'-monophosphoramidase activity [PMID:11805111]
EXISTENCE:mutant phenotype:GO:0010043 response to zinc ion [PMID:31692084]
Adenosine 5'-monophosphoramidase; interacts physically and genetically with CDK and TFIIK subunit Kin28p, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant
EXISTENCE:direct assay:GO:0004032 alditol:NADP+ 1-oxidoreductase activity [PMID:11306085]
EXISTENCE:direct assay:GO:0004033 aldo-keto reductase (NADP) activity [PMID:17140678]
EXISTENCE:direct assay:GO:0006725 cellular aromatic compound metabolic process [PMID:16268655]
EXISTENCE:direct assay:GO:0042180 cellular ketone metabolic process [PMID:17140678]
EXISTENCE:direct assay:GO:0043603 amide metabolic process [PMID:15564669]
EXISTENCE:direct assay:GO:0051268 alpha-keto amide reductase activity [PMID:15564669]
EXISTENCE:direct assay:GO:0051269 alpha-keto ester reductase activity [PMID:15564669]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:17919749]
NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:19168755]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11566881|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000328 fungal-type vacuole lumen [PMID:19168755]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:19168755]
hypothetical protein; localized to the vacuolar outer membrane; predicted to be palmitoylated
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:1429680]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:1429680]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15635103]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15635103|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:31713515]
EXISTENCE:direct assay:GO:0016579 protein deubiquitination [PMID:1429680|PMID:2050695]
EXISTENCE:direct assay:GO:1905524 negative regulation of protein autoubiquitination [PMID:31713515]
Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10684247|PMID:14562095|PMID:26928762]
A cargo receptor protein for Pma1p; works with Psg1p to promote the transport and maturation of Pma1p; localizes to the ER and COPII vesicles; a non-essential protein
EXISTENCE:direct assay:GO:0004322 ferroxidase activity [PMID:12149269|PMID:12732649]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:10428860]
EXISTENCE:direct assay:GO:0006879 cellular iron ion homeostasis [PMID:9180083]
EXISTENCE:direct assay:GO:0008198 ferrous iron binding [PMID:10930361|PMID:16784228]
EXISTENCE:direct assay:GO:0016226 iron-sulfur cluster assembly [PMID:12947415]
EXISTENCE:direct assay:GO:0034986 iron chaperone activity [PMID:12732649]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:11734220|PMID:9180083]
EXISTENCE:genetic interaction:GO:0016226 iron-sulfur cluster assembly [PMID:14741370]
EXISTENCE:genetic interaction:GO:0034986 iron chaperone activity [PMID:15247478]
EXISTENCE:mutant phenotype:GO:0006749 glutathione metabolic process [PMID:18562474]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:11734220|PMID:9180083]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:12221295|PMID:12947415|PMID:12970193]
EXISTENCE:physical interaction:GO:0016226 iron-sulfur cluster assembly [PMID:14741370]
Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog FXN is mutated in Friedrich's ataxia; human FTL gene can complement yeast yfh1 null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:27476175|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0015187 glycine transmembrane transporter activity [PMID:27476175]
EXISTENCE:genetic interaction:GO:0006783 heme biosynthetic process [PMID:26821380]
EXISTENCE:genetic interaction:GO:0015187 glycine transmembrane transporter activity [PMID:26821380]
EXISTENCE:genetic interaction:GO:1904983 glycine import into mitochondrion [PMID:26821380]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:26821380|PMID:27476175]
EXISTENCE:mutant phenotype:GO:0015187 glycine transmembrane transporter activity [PMID:26821380|PMID:27476175]
EXISTENCE:mutant phenotype:GO:1904983 glycine import into mitochondrion [PMID:26821380|PMID:27476175]
Mitochondrial glycine transporter; required for the transport of glycine into mitochondria for initiation of heme biosynthesis, with YMC1 acting as a secondary transporter; homolog of human SLC25A38, a mitochondrial glycine transporter associated with nonsyndromic autosomal recessive congenital sideroblastic anemia; human SLC25A38 can complement the heme deficiency associated with the null mutant; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:21498574]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:21498574|PMID:14562095|PMID:22842922]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:22956544]
EXISTENCE:genetic interaction:GO:1990344 secondary cell septum biogenesis [PMID:19528296]
EXISTENCE:physical interaction:GO:0044697 HICS complex [PMID:22623719]
EXISTENCE:physical interaction:GO:1990344 secondary cell septum biogenesis [PMID:19528296]
SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21818277]
EXISTENCE:direct assay:GO:0031080 nuclear pore outer ring [PMID:11823431|PMID:18046406]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:15725626]
EXISTENCE:genetic interaction:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:16418532|PMID:17538013]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:8565072]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:12730220]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:22331846]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:16418532]
EXISTENCE:mutant phenotype:GO:0045893 positive regulation of DNA-templated transcription [PMID:15817685]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22110603]
EXISTENCE:mutant phenotype:GO:0051664 nuclear pore localization [PMID:8565072]
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
ARS410; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:21504834|PMID:23267056]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:21504834]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:21504834]
RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene
ARS411; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:12048214|PMID:15659643|PMID:18497569]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12554655]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:12048214|PMID:11557775]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:12554655]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:12048214]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:12554655]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:12554655]
EXISTENCE:physical interaction:GO:0034498 early endosome to Golgi transport [PMID:12554655]
Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway, endosomal sorting and selective autophagy; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
tRNA (guanosine(18)-2'-O)-methyltransferase
EXISTENCE:direct assay:GO:0016423 tRNA (guanine) methyltransferase activity [PMID:9917067]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:9917067]
EXISTENCE:mutant phenotype:GO:0002128 tRNA nucleoside ribose methylation [PMID:32053677]
2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
exosome non-catalytic core subunit RRP42
EXISTENCE:curator inference:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:10465791]
EXISTENCE:curator inference:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10465791]
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:10465791]
EXISTENCE:curator inference:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10508172|PMID:9390555]
EXISTENCE:mutant phenotype:GO:0016075 rRNA catabolic process [PMID:10508172]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
EXISTENCE:direct assay:GO:0005835 fatty acid synthase complex [PMID:32160528]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:25042801]
EXISTENCE:direct assay:GO:0070682 proteasome regulatory particle assembly [PMID:25042801]
EXISTENCE:direct assay:GO:0098772 molecular function regulator activity [PMID:32160528]
EXISTENCE:mutant phenotype:GO:0006633 fatty acid biosynthetic process [PMID:32160528]
Regulatory gamma subunit of fatty acid synthase (FAS); ATPase dedicated chaperone that adapts proteasome assembly to stress; induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication
TFIIH complex serine/threonine-protein kinase subunit KIN28
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:19818408|PMID:27381459]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:19818408]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:direct assay:GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity [PMID:7760796|PMID:9702190]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:14749387]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:11839796|PMID:19450536|PMID:19679665|PMID:7760796|PM ID:9702190]
EXISTENCE:direct assay:GO:0070985 transcription factor TFIIK complex [PMID:11839796|PMID:19818408]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:12015980]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:7760796|PMID:7783209]
EXISTENCE:mutant phenotype:GO:0006370 7-methylguanosine mRNA capping [PMID:22689984]
EXISTENCE:mutant phenotype:GO:0006995 cellular response to nitrogen starvation [PMID:26567215]
EXISTENCE:mutant phenotype:GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity [PMID:9702190]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:26567215]
EXISTENCE:mutant phenotype:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:9702190]
EXISTENCE:mutant phenotype:GO:1900018 phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex [PMID:10594013|PMID:19666497]
EXISTENCE:mutant phenotype:GO:1901921 phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [PMID:10594013]
EXISTENCE:mutant phenotype:GO:1903654 phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter [PMID:27477907]
EXISTENCE:mutant phenotype:GO:1905866 positive regulation of Atg1/ULK1 kinase complex assembly [PMID:26567215]
EXISTENCE:physical interaction:GO:1901921 phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [PMID:10594013]
Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:11604502]
EXISTENCE:genetic interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11604502]
EXISTENCE:physical interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:11950926|PMID:17452441]
Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:2664469]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:3303332|PMID:9566882|PMID:7902583]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1493793|PMID:22932476]
EXISTENCE:direct assay:GO:0006141 regulation of purine nucleobase metabolic process [PMID:1495962]
EXISTENCE:direct assay:GO:0043388 positive regulation of DNA binding [PMID:8355698]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:genetic interaction:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9566882|PMID:7902583]
EXISTENCE:genetic interaction:GO:0045937 positive regulation of phosphate metabolic process [PMID:3915785]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3915785|PMID:7902583]
EXISTENCE:genetic interaction:GO:0120213 regulation of histidine biosynthetic process [PMID:3303332]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:3303332|PMID:7902583|PMID:9566882]
EXISTENCE:mutant phenotype:GO:0006141 regulation of purine nucleobase metabolic process [PMID:1495962]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:2196175]
EXISTENCE:mutant phenotype:GO:0043388 positive regulation of DNA binding [PMID:7902583]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3303332|PMID:7902583]
EXISTENCE:mutant phenotype:GO:0120213 regulation of histidine biosynthetic process [PMID:3303332]
EXISTENCE:mutant phenotype:GO:2000679 positive regulation of transcription regulatory region DNA binding [PMID:9354395]
Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:15738391]
EXISTENCE:direct assay:GO:0030915 Smc5-Smc6 complex [PMID:15738391]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391|PMID:15752197]
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
putative N(6)-L-threonylcarbamoyladenine synthase
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:33277478]
EXISTENCE:direct assay:GO:0002949 tRNA threonylcarbamoyladenosine modification [PMID:23620299]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
EXISTENCE:direct assay:GO:0061711 N(6)-L-threonylcarbamoyladenine synthase activity [PMID:23620299]
EXISTENCE:mutant phenotype:GO:0072670 mitochondrial tRNA threonylcarbamoyladenosine modification [PMID:21183954]
Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex
EXISTENCE:direct assay:GO:0003977 UDP-N-acetylglucosamine diphosphorylase activity [PMID:9603950]
EXISTENCE:direct assay:GO:0006048 UDP-N-acetylglucosamine biosynthetic process [PMID:9603950]
UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:1678279|PMID:18635534]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:15773893]
EXISTENCE:direct assay:GO:0006278 RNA-templated DNA biosynthetic process [PMID:17429354]
EXISTENCE:direct assay:GO:0043137 DNA replication, removal of RNA primer [PMID:16837458]
EXISTENCE:genetic interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18606574]
EXISTENCE:genetic interaction:GO:0045005 DNA-templated DNA replication maintenance of fidelity [PMID:15601866]
EXISTENCE:mutant phenotype:GO:0003887 DNA-directed DNA polymerase activity [PMID:1678279]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:1678279]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:18606574]
EXISTENCE:mutant phenotype:GO:0008296 3'-5'-exodeoxyribonuclease activity [PMID:1648480]
EXISTENCE:mutant phenotype:GO:0045004 DNA replication proofreading [PMID:32123096]
Catalytic subunit of DNA polymerase delta; responsible for most of lagging strand DNA synthesis; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:11904430|PMID:16319894]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:24647101]
EXISTENCE:genetic interaction:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:34244791]
EXISTENCE:genetic interaction:GO:0048478 replication fork protection [PMID:17158920]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:11904430]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:24647101]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:34244791]
Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:18261907]
EXISTENCE:direct assay:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:20850366]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12680698]
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:25242142]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:25242142]
EXISTENCE:genetic interaction:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:18261907]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:16269340]
EXISTENCE:mutant phenotype:GO:0009408 response to heat [PMID:12680698]
EXISTENCE:mutant phenotype:GO:0010038 response to metal ion [PMID:12680698]
EXISTENCE:mutant phenotype:GO:0045048 protein insertion into ER membrane [PMID:18724936]
EXISTENCE:physical interaction:GO:0043529 GET complex [PMID:16269340]
Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:17261844]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17261844]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17261844]
EXISTENCE:mutant phenotype:GO:0009306 protein secretion [PMID:20156967]
EXISTENCE:physical interaction:GO:0005801 cis-Golgi network [PMID:17261844]
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:16269340]
Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10449419|PMID:10377396]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10449419|PMID:11720284]
Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome
proteasome regulatory particle lid subunit RPN6
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:9741626]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12486135]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:12486135]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:12486135]
Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
dolichyl-phosphate-mannose-protein mannosyltransferase PMT1
EXISTENCE:direct assay:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:21956107|PMID:8367478|PMID:8543034]
EXISTENCE:direct assay:GO:0035269 protein O-linked mannosylation [PMID:8367478|PMID:8543034]
EXISTENCE:direct assay:GO:0097582 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex [PMID:12551906]
EXISTENCE:direct assay:GO:0097583 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex [PMID:12551906]
EXISTENCE:genetic interaction:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:8543034]
EXISTENCE:genetic interaction:GO:0032527 protein exit from endoplasmic reticulum [PMID:21147851]
EXISTENCE:genetic interaction:GO:0035269 protein O-linked mannosylation [PMID:8543034|PMID:21231968]
EXISTENCE:genetic interaction:GO:0071712 ER-associated misfolded protein catabolic process [PMID:18182384|PMID:21147851]
EXISTENCE:genetic interaction:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:21231968|PMID:23704572]
EXISTENCE:mutant phenotype:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:8367478|PMID:8543034]
EXISTENCE:mutant phenotype:GO:0035269 protein O-linked mannosylation [PMID:8367478|PMID:18182384|PMID:8543034]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:18182384]
EXISTENCE:mutant phenotype:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:23704572]
EXISTENCE:physical interaction:GO:0097582 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex [PMID:21956107|PMID:8543034]
Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; functions as a heterodimer with Pmt2p but can also pair with Pmt3p; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
putative dolichyl-phosphate-mannose-protein mannosyltransferase PMT5
EXISTENCE:direct assay:GO:0097584 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex [PMID:12551906]
EXISTENCE:direct assay:GO:0097585 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex [PMID:12551906]
Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals
RNA-binding signal recognition particle subunit SRP14
EXISTENCE:direct assay:GO:0005786 signal recognition particle, endoplasmic reticulum targeting [PMID:7925282]
EXISTENCE:direct assay:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:10921896]
EXISTENCE:direct assay:GO:0008312 7S RNA binding [PMID:10573124]
Signal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:26362318]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:26362318]
EXISTENCE:direct assay:GO:0044695 Dsc E3 ubiquitin ligase complex [PMID:22681890]
EXISTENCE:mutant phenotype:GO:0033523 histone H2B ubiquitination [PMID:25183520]
EXISTENCE:mutant phenotype:GO:0072583 clathrin-dependent endocytosis [PMID:26362318]
Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10
EXISTENCE:direct assay:GO:0004660 protein farnesyltransferase activity [PMID:9380709]
EXISTENCE:direct assay:GO:0005965 protein farnesyltransferase complex [PMID:8424764]
EXISTENCE:direct assay:GO:0018343 protein farnesylation [PMID:1763050]
Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:25569132]
EXISTENCE:mutant phenotype:GO:0043007 maintenance of rDNA [PMID:18997772]
Chromosome linkage inner nuclear membrane protein; CLIP, involved in perinuclear chromosome tethering and rDNA repeat stability; involved in regulating mitotic exit; dephosphorylated by Cdc14p in anaphase, which promotes timely rDNA segregation and allows mitotic progression; interacts with Csm1p and Lrs4p; null mutant has elevated levels of unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery; Cdc28p substrate
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247|PMID:9864357]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:genetic interaction:GO:0006999 nuclear pore organization [PMID:19414609]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:17726058]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:mutant phenotype:GO:0003729 mRNA binding [PMID:17726058]
EXISTENCE:mutant phenotype:GO:0005685 U1 snRNP [PMID:10500099]
EXISTENCE:mutant phenotype:GO:0006376 mRNA splice site selection [PMID:10500099]
Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia
Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
Homolog of modifier of aggregation-4 (MOAG-4) from worm and small EDRK-rich factor protein (SERF1a) from human; highly conserved enhancer of amyloid formation of Abeta40 and alpha-synuclein both in vitro and in vivo; protein displays very high degree of disorder, segment spanning residues 36E-65K has strong helical propensity
NADH-ubiquinone reductase (H(+)-translocating) NDE2
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006116 NADH oxidation [PMID:12032156]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9733747]
EXISTENCE:mutant phenotype:GO:0019655 glycolytic fermentation to ethanol [PMID:9733747]
Mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000346 transcription export complex [PMID:11979277]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:15942929]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:28059701]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0006406 mRNA export from nucleus [PMID:11675790|PMID:11696331]
EXISTENCE:genetic interaction:GO:0003724 RNA helicase activity [PMID:11156602]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:11156602]
EXISTENCE:mutant phenotype:GO:0006283 transcription-coupled nucleotide-excision repair [PMID:17537816]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17960421]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:11675790|PMID:11696331]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:18614048]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:15942929]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:11675790]
Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:genetic interaction:GO:0006413 translational initiation [PMID:30481328]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:24391917]
EXISTENCE:direct assay:GO:0045860 positive regulation of protein kinase activity [PMID:24391917]
Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
branched-chain-2-oxoacid decarboxylase THI3
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16194233|PMID:16850348|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16194233]
EXISTENCE:mutant phenotype:GO:0090180 positive regulation of thiamine biosynthetic process [PMID:16194233|PMID:19013460]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:19013460]
EXISTENCE:physical interaction:GO:0090180 positive regulation of thiamine biosynthetic process [PMID:16194233|PMID:19013460]
Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected
putative serine/threonine protein kinase MRK1
EXISTENCE:genetic interaction:GO:0042176 regulation of protein catabolic process [PMID:10958669]
Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:21124907]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:1447211|PMID:7628449|PMID:22375831]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:8824293]
EXISTENCE:direct assay:GO:0030060 L-malate dehydrogenase activity [PMID:1447211]
EXISTENCE:mutant phenotype:GO:0006635 fatty acid beta-oxidation [PMID:7628449]
EXISTENCE:mutant phenotype:GO:0006735 NADH regeneration [PMID:7628449]
EXISTENCE:mutant phenotype:GO:0030060 L-malate dehydrogenase activity [PMID:1986231|PMID:7628449]
Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle; mutation in human homolog MDH2 causes early-onset severe encephalopathy
ARS412; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11853670]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19748353|PMID:9111041|PMID:26928762]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:19748353]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:16601699]
EXISTENCE:direct assay:GO:0035542 regulation of SNARE complex assembly [PMID:18385512]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:direct assay:GO:0099022 vesicle tethering [PMID:27307091]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026035|PMID:25026036]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:9111041]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:12730205]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:10944212]
EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:19748353]
EXISTENCE:physical interaction:GO:0030897 HOPS complex [PMID:10944212]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:11062257|PMID:18809726|PMID:20604902|PMID:21325627]
Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:24390141]
EXISTENCE:direct assay:GO:0033698 Rpd3L complex [PMID:16286007|PMID:16314178|PMID:19040720]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0016479 negative regulation of transcription by RNA polymerase I [PMID:19270272]
Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
EXISTENCE:mutant phenotype:GO:0006450 regulation of translational fidelity [PMID:18829863]
Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:11081632]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A2256
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12876294]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:23103252]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:19531475]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:23390378]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:16783014]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:15632065]
EXISTENCE:mutant phenotype:GO:0010390 histone monoubiquitination [PMID:12535538|PMID:12535539]
EXISTENCE:mutant phenotype:GO:0016574 histone ubiquitination [PMID:23103252]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:23103252]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:12535539]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:16166626|PMID:16783014]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:15632126]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:15280549]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:19531475]
EXISTENCE:mutant phenotype:GO:0070534 protein K63-linked ubiquitination [PMID:25622294]
EXISTENCE:physical interaction:GO:0006366 transcription by RNA polymerase II [PMID:19531475]
E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing
EXISTENCE:genetic interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:26787842]
EXISTENCE:genetic interaction:GO:0043407 negative regulation of MAP kinase activity [PMID:26787842]
EXISTENCE:physical interaction:GO:0005078 MAP-kinase scaffold activity [PMID:26787842]
EXISTENCE:physical interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:26787842]
Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway; physically interacts with the cytoplasmic domain of Hkr1p, and with Sho1p, Pbs2p, and Ste11p; prevents cross-talk signaling from Hkr1p of the osmotic stress MAPK cascade to the Kss1p MAPK cascade; non-essential gene
ARS413; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:20378542|PMID:11914276|PMID:26928762]
hypothetical protein; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:17526728]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:12620224]
EXISTENCE:direct assay:GO:0070577 lysine-acetylated histone binding [PMID:20126658]
EXISTENCE:genetic interaction:GO:0031452 negative regulation of heterochromatin formation [PMID:16239142]
EXISTENCE:mutant phenotype:GO:0031452 negative regulation of heterochromatin formation [PMID:16239142]
EXISTENCE:physical interaction:GO:0001094 TFIID-class transcription factor complex binding [PMID:10783167]
Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:1745228]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:3033440]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:3033440]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:3033440]
EXISTENCE:physical interaction:GO:0005761 mitochondrial ribosome [PMID:15927884]
Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
EXISTENCE:direct assay:GO:0004129 cytochrome-c oxidase activity [PMID:1331058|PMID:7851399]
EXISTENCE:direct assay:GO:0005751 mitochondrial respiratory chain complex IV [PMID:1331058]
EXISTENCE:direct assay:GO:0006123 mitochondrial electron transport, cytochrome c to oxygen [PMID:1331058]
Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
EXISTENCE:direct assay:GO:0004450 isocitrate dehydrogenase (NADP+) activity [PMID:15574419]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15001388|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:genetic interaction:GO:0006537 glutamate biosynthetic process [PMID:15574419]
EXISTENCE:mutant phenotype:GO:0004450 isocitrate dehydrogenase (NADP+) activity [PMID:8099357]
Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:9418908]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16009135]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9418908|PMID:26928762]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:16679311]
EXISTENCE:mutant phenotype:GO:0032527 protein exit from endoplasmic reticulum [PMID:16009135]
EXISTENCE:mutant phenotype:GO:0032581 ER-dependent peroxisome organization [PMID:20427571]
EXISTENCE:mutant phenotype:GO:0045046 protein import into peroxisome membrane [PMID:10637226]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16679311|PMID:9418908]
EXISTENCE:physical interaction:GO:0033328 peroxisome membrane targeting sequence binding [PMID:16679311]
EXISTENCE:physical interaction:GO:0045033 peroxisome inheritance [PMID:22486971]
EXISTENCE:physical interaction:GO:0050821 protein stabilization [PMID:16679311]
Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16782016]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7800043]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:9341106|PMID:9435231]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:9341106|PMID:9435231]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:7800043]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:9341106|PMID:9435231]
EXISTENCE:mutant phenotype:GO:0019789 SUMO transferase activity [PMID:9435231]
SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:26112308]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:23118189]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:19806183]
Transport adaptor or symportin; assembly chaperone that co-translationally associates with nascent Rpl5p, preventing aggregation; facilitates synchronized nuclear coimport of two 5S-rRNA binding proteins, Rpl5p and Rpl11p, mediated by import receptor Kap104p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:18782943|PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480|PMID:18782943]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480|PMID:18782943]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11565749]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15167894|PMID:22842922]
EXISTENCE:direct assay:GO:0043021 ribonucleoprotein complex binding [PMID:15167894]
EXISTENCE:mutant phenotype:GO:0000461 endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11565749]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15167894|PMID:12089522]
Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:26540255]
EXISTENCE:genetic interaction:GO:0005634 nucleus [PMID:12368472]
EXISTENCE:genetic interaction:GO:0045002 double-strand break repair via single-strand annealing [PMID:11606529]
EXISTENCE:genetic interaction:GO:2000278 regulation of DNA biosynthetic process [PMID:23824283]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:11238918]
EXISTENCE:mutant phenotype:GO:0006277 DNA amplification [PMID:23271978]
EXISTENCE:mutant phenotype:GO:0043504 mitochondrial DNA repair [PMID:26540255]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10655498]
Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:17192843]
EXISTENCE:direct assay:GO:0012507 ER to Golgi transport vesicle membrane [PMID:17192843]
EXISTENCE:direct assay:GO:0048211 Golgi vesicle docking [PMID:9545229]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:8603910]
EXISTENCE:mutant phenotype:GO:0035493 SNARE complex assembly [PMID:8603910]
Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
hypothetical protein; YDL057W is not an essential gene
EXISTENCE:direct assay:GO:0030907 MBF transcription complex [PMID:8372350]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8372350]
EXISTENCE:mutant phenotype:GO:0071931 positive regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:8372350]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:30465652]
Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
EXISTENCE:direct assay:GO:0004475 mannose-1-phosphate guanylyltransferase (GTP) activity [PMID:9195935]
EXISTENCE:direct assay:GO:0009298 GDP-mannose biosynthetic process [PMID:9195935]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:11055399]
EXISTENCE:mutant phenotype:GO:0004475 mannose-1-phosphate guanylyltransferase (GTP) activity [PMID:9195935]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:9195935]
EXISTENCE:mutant phenotype:GO:0009298 GDP-mannose biosynthetic process [PMID:9195935]
GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase); synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:19001347|PMID:26928762]
Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress; also detected in peroxisomes
1-acylglycerol-3-phosphate O-acyltransferase SLC1
EXISTENCE:direct assay:GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity [PMID:9212466]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093|PMID:10515935]
EXISTENCE:genetic interaction:GO:0046474 glycerophospholipid biosynthetic process [PMID:17675291]
1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:19749380]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:7799435]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10564276]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:11720288]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11720288]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9150139]
RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:8810042]
EXISTENCE:mutant phenotype:GO:0006078 (1->6)-beta-D-glucan biosynthetic process [PMID:8810042]
Protein with similarity to Kre9p; Kre9p is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication
type 2A-related serine/threonine-protein phosphatase SIT4
EXISTENCE:direct assay:GO:0000159 protein phosphatase type 2A complex [PMID:8600023]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:1848673|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:1848673]
EXISTENCE:mutant phenotype:GO:0000159 protein phosphatase type 2A complex [PMID:8600023]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:23864707]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15150670]
EXISTENCE:mutant phenotype:GO:0032956 regulation of actin cytoskeleton organization [PMID:12080055]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:18357452]
EXISTENCE:mutant phenotype:GO:0035556 intracellular signal transduction [PMID:12080055]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:12080055]
EXISTENCE:mutant phenotype:GO:1903432 regulation of TORC1 signaling [PMID:10604478|PMID:12820961]
Ceramide-activated, type 2A-related serine-threonine phosphatase; functions in G1/S transition of mitotic cycle; controls lifespan, mitochondrial function, cell cycle progression by regulating HXK2 phosphorylation; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:16278452]
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:33141558]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:31543266]
EXISTENCE:direct assay:GO:0031210 phosphatidylcholine binding [PMID:33141558]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:31543266]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:33141558]
EXISTENCE:genetic interaction:GO:0032366 intracellular sterol transport [PMID:16278452]
Sterol transport protein, functional homolog of human NPC2 Niemann-Pick disease type C2 protein; together with Ncr1p mediates integration of ergosterol into vacuolar membrane; functions as general 'lipid solubilizer', binds variety of amphiphilic lipid ligands; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; yeast NPC2 can complement mutations in human NPC2
mitochondrial 37S ribosomal protein YmS-T
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9065385|PMID:9151978]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:9065385|PMID:9151978]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:9065385]
Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria
EXISTENCE:direct assay:GO:0003919 FMN adenylyltransferase activity [PMID:7799934]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:7799934]
EXISTENCE:direct assay:GO:0006747 FAD biosynthetic process [PMID:7799934]
EXISTENCE:mutant phenotype:GO:0006747 FAD biosynthetic process [PMID:7799934]
Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8200349|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0003723 RNA binding [PMID:8413192]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:8413192]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:12637560]
Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
EXISTENCE:direct assay:GO:0000245 spliceosomal complex assembly [PMID:8969185]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:8065365]
Subunit of the SF3a splicing factor complex; required for spliceosome assembly
EXISTENCE:direct assay:GO:0000792 heterochromatin [PMID:20176978]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:29795547]
EXISTENCE:direct assay:GO:0005677 chromatin silencing complex [PMID:9122169]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9214640]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:11553718]
EXISTENCE:direct assay:GO:0017136 NAD-dependent histone deacetylase activity [PMID:10811920]
EXISTENCE:direct assay:GO:0030869 RENT complex [PMID:10219244]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:19217406]
EXISTENCE:direct assay:GO:0032041 NAD-dependent histone H3K14 deacetylase activity [PMID:10693811]
EXISTENCE:direct assay:GO:0046969 NAD-dependent histone H3K9 deacetylase activity [PMID:10693811]
EXISTENCE:direct assay:GO:0046970 NAD-dependent histone H4K16 deacetylase activity [PMID:10693811]
EXISTENCE:genetic interaction:GO:0006325 chromatin organization [PMID:26951198]
EXISTENCE:genetic interaction:GO:0045910 negative regulation of DNA recombination [PMID:16182251]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:2647300]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:27122604]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:9501103]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:27185881]
EXISTENCE:mutant phenotype:GO:0008156 negative regulation of DNA replication [PMID:15082529]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11950950|PMID:3297920]
EXISTENCE:mutant phenotype:GO:0031494 regulation of mating type switching [PMID:31461456]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:1913809|PMID:9501103|PMID:11950950]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:27122604]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:25822194]
EXISTENCE:mutant phenotype:GO:0097695 establishment of protein-containing complex localization to telomere [PMID:26218225]
EXISTENCE:mutant phenotype:GO:0097752 regulation of DNA stability [PMID:27820830]
EXISTENCE:mutant phenotype:GO:1904524 negative regulation of DNA amplification [PMID:26195783]
Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication, suppressing early firing of multicopy rDNA origins; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy
peptide alpha-N-acetyltransferase complex A subunit NAT1
EXISTENCE:direct assay:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:2551674]
EXISTENCE:direct assay:GO:0006474 N-terminal protein amino acid acetylation [PMID:2551674]
EXISTENCE:direct assay:GO:0031415 NatA complex [PMID:14517307]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:14517307]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:2551674]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:2551674]
Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs
Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the IMI1
Protein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the gene IMI1
EXISTENCE:direct assay:GO:0009982 pseudouridine synthase activity [PMID:15466869]
EXISTENCE:direct assay:GO:0031119 tRNA pseudouridine synthesis [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0009982 pseudouridine synthase activity [PMID:15466869]
EXISTENCE:mutant phenotype:GO:0031119 tRNA pseudouridine synthesis [PMID:15466869]
Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10655215|PMID:9524122|PMID:9388468]
EXISTENCE:genetic interaction:GO:0009757 hexose mediated signaling [PMID:9813141]
EXISTENCE:genetic interaction:GO:0010255 glucose mediated signaling pathway [PMID:10361302]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0004930 G protein-coupled receptor activity [PMID:9524122]
EXISTENCE:mutant phenotype:GO:0005536 glucose binding [PMID:15494315]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:10655215]
EXISTENCE:mutant phenotype:GO:0007186 G protein-coupled receptor signaling pathway [PMID:9524122]
EXISTENCE:mutant phenotype:GO:0009731 detection of sucrose stimulus [PMID:15494315]
EXISTENCE:mutant phenotype:GO:0009745 sucrose mediated signaling [PMID:15494315]
EXISTENCE:mutant phenotype:GO:0009757 hexose mediated signaling [PMID:10655215|PMID:9813141]
EXISTENCE:mutant phenotype:GO:0010255 glucose mediated signaling pathway [PMID:10361302|PMID:15494315|PMID:9813141]
EXISTENCE:mutant phenotype:GO:0051594 detection of glucose [PMID:15494315]
EXISTENCE:physical interaction:GO:0004930 G protein-coupled receptor activity [PMID:9388468|PMID:9524122]
EXISTENCE:physical interaction:GO:0007186 G protein-coupled receptor signaling pathway [PMID:9388468|PMID:9524122]
Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
tRNA-5-taurinomethyluridine 2-sulfurtransferase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15944150|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase [PMID:15509579]
EXISTENCE:mutant phenotype:GO:1990799 mitochondrial tRNA wobble position uridine thiolation [PMID:15509579]
tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
putative ATP-dependent RNA helicase DBP10
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10871363]
EXISTENCE:genetic interaction:GO:0030687 preribosome, large subunit precursor [PMID:16803892]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10871363]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10871363]
EXISTENCE:mutant phenotype:GO:1902626 assembly of large subunit precursor of preribosome [PMID:22735702]
EXISTENCE:physical interaction:GO:0030687 preribosome, large subunit precursor [PMID:16095611|PMID:16803892]
Putative ATP-dependent RNA helicase of the DEAD-box protein family; constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
SF3a splicing factor complex subunit PRP9
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:8969185]
EXISTENCE:mutant phenotype:GO:0000395 mRNA 5'-splice site recognition [PMID:2147224]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:2147224]
Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:15657399]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11248049]
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:11248049]
EXISTENCE:mutant phenotype:GO:0005524 ATP binding [PMID:15657399]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:16862144]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:17118118]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:18287533]
EXISTENCE:mutant phenotype:GO:0034314 Arp2/3 complex-mediated actin nucleation [PMID:16862144]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:10377407]
EXISTENCE:mutant phenotype:GO:0051654 establishment of mitochondrion localization [PMID:16107558]
Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants
serine/threonine/tyrosine protein kinase MPS1
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11827982]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11278681|PMID:25382489]
EXISTENCE:direct assay:GO:0004712 protein serine/threonine/tyrosine kinase activity [PMID:7737118]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:11827982]
EXISTENCE:direct assay:GO:0043515 kinetochore binding [PMID:31405987]
EXISTENCE:genetic interaction:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:8567717]
EXISTENCE:genetic interaction:GO:0034501 protein localization to kinetochore [PMID:24402315]
EXISTENCE:mutant phenotype:GO:0004712 protein serine/threonine/tyrosine kinase activity [PMID:15668173]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:8567717|PMID:32307893]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:1869587]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:18060784]
EXISTENCE:mutant phenotype:GO:0051225 spindle assembly [PMID:15668173]
EXISTENCE:mutant phenotype:GO:0051988 regulation of attachment of spindle microtubules to kinetochore [PMID:23334295]
Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); detected in peroxisomes; similar to human Mps1p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:35779633]
Mitochondrial inner membrane protein; high copy suppressor of ER stress-mediated cell death; overexpression increases O2 consumption, impairs processing of COB and COX1 RNAs encoding mitochondrial oxidative phosphorylation factors, and impacts the transition from fermentative to respiratory metabolism
putative serine/threonine protein kinase RTK1
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:23785265]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:23785265]
Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
ARS414; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11935221]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:11063681]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:11063681]
hypothetical protein; involved in invasive and pseudohyphal growth
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partially overlaps the verified gene DIA3; identified by fungal homology and RT-PCR; mRNA identified as translated by ribosome profiling data
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon AGC (converted to IGC in the mature tRNA), decodes GCU and GCC codons into alanine, one of 16 nuclear tRNAs for alanine
glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20026609]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:15210723]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15210723]
EXISTENCE:mutant phenotype:GO:0006116 NADH oxidation [PMID:9171333]
EXISTENCE:mutant phenotype:GO:0006973 intracellular accumulation of glycerol [PMID:8196651]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:26276932]
EXISTENCE:mutant phenotype:GO:0047952 glycerol-3-phosphate dehydrogenase [NAD(P)+] activity [PMID:8196651]
NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import
phosphoglycerate mutase family protein GPM2
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24146988|PMID:11914276]
Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM2 has a paralog, GPM3, that arose from the whole genome duplication
stress-regulated transcription factor RPN4
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10350051]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10350051]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19914394|PMID:8514125|PMID:22842922]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10350051|PMID:19914394]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15178333]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:20376190|PMID:11027285]
EXISTENCE:mutant phenotype:GO:0006282 regulation of DNA repair [PMID:11027285|PMID:20376190]
Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
oxysterol-binding protein related protein OSH2
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:25420878]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12727870]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11408574|PMID:25420878]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11408574]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:20008566]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:20008566]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:20008566]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:mutant phenotype:GO:0035621 ER to Golgi ceramide transport [PMID:24213531]
Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane and at the nuclear envelope; regulated by sterol binding; OSH2 has a paralog, SWH1, that arose from the whole genome duplication
Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:23746350]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10508166]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2023904]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:34432494]
EXISTENCE:direct assay:GO:0031431 Dbf4-dependent protein kinase complex [PMID:19692334]
EXISTENCE:direct assay:GO:1902977 mitotic DNA replication preinitiation complex assembly [PMID:21729781|PMID:26338774]
EXISTENCE:genetic interaction:GO:0031431 Dbf4-dependent protein kinase complex [PMID:8474449]
EXISTENCE:genetic interaction:GO:0031503 protein-containing complex localization [PMID:19013276]
EXISTENCE:genetic interaction:GO:0033314 mitotic DNA replication checkpoint signaling [PMID:18372119]
EXISTENCE:genetic interaction:GO:0060903 positive regulation of meiosis I [PMID:18245451]
EXISTENCE:genetic interaction:GO:1904968 positive regulation of monopolar spindle attachment to meiosis I kinetochore [PMID:19013276]
EXISTENCE:genetic interaction:GO:1905263 positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination [PMID:18245451|PMID:19013276]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:2023904|PMID:2166954|PMID:10508166|PMID:10805723|PMI D:18245450]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:9472018]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:16319063]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:19013276]
EXISTENCE:mutant phenotype:GO:1902977 mitotic DNA replication preinitiation complex assembly [PMID:21729781]
EXISTENCE:mutant phenotype:GO:1904968 positive regulation of monopolar spindle attachment to meiosis I kinetochore [PMID:19013276]
EXISTENCE:mutant phenotype:GO:1905263 positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination [PMID:18245450]
EXISTENCE:mutant phenotype:GO:1905342 positive regulation of protein localization to kinetochore [PMID:34432494]
EXISTENCE:physical interaction:GO:0001100 negative regulation of exit from mitosis [PMID:19478884]
EXISTENCE:physical interaction:GO:0031431 Dbf4-dependent protein kinase complex [PMID:8474449]
S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45 to drive assembly of the pre-IC and the CMG helicase; maintains Cse4p at kinetochores through phosphorylation to regulate chromosome segregation, regulates pre-meiotic replication, meiotic DSB formation, monopolar attachment of homologs during MI through monopolin recruitment and middle meiotic gene expression; HsCDC7 and DBF4 complement
trans-2-enoyl-CoA reductase (NADPH) TSC13
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11113186]
EXISTENCE:mutant phenotype:GO:0000038 very long-chain fatty acid metabolic process [PMID:11113186]
EXISTENCE:mutant phenotype:GO:0016491 oxidoreductase activity [PMID:11113186]
Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11932453|PMID:2686980]
EXISTENCE:direct assay:GO:0008649 rRNA methyltransferase activity [PMID:12215523]
EXISTENCE:direct assay:GO:0031167 rRNA methylation [PMID:12215523]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:11081632]
EXISTENCE:direct assay:GO:1990258 histone glutamine methylation [PMID:24352239]
EXISTENCE:mutant phenotype:GO:0000494 box C/D RNA 3'-end processing [PMID:10733567|PMID:15167896]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:1825809]
EXISTENCE:mutant phenotype:GO:0008649 rRNA methyltransferase activity [PMID:12215523]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:12215523|PMID:8431947]
EXISTENCE:mutant phenotype:GO:0043144 sno(s)RNA processing [PMID:1825809]
EXISTENCE:mutant phenotype:GO:1990258 histone glutamine methylation [PMID:24352239]
EXISTENCE:mutant phenotype:GO:1990259 histone glutamine N-methyltransferase activity [PMID:24352239]
Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin; inviability of the null mutant is functionally complemented by human FBL
SUMO-targeted ubiquitin ligase complex subunit SLX5
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:23785440]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:17728242|PMID:17848550|PMID:18032921]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19270524|PMID:23785440]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:17728242|PMID:17848550|PMID:18032921|PMID:26906737]
EXISTENCE:direct assay:GO:0032183 SUMO binding [PMID:17848550]
EXISTENCE:direct assay:GO:0033768 SUMO-targeted ubiquitin ligase complex [PMID:17848550]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495]
EXISTENCE:genetic interaction:GO:0016567 protein ubiquitination [PMID:26906737]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:16387868]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:16428246]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:26960795]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:31320817]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495|PMID:16325482]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:26906737]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:16387868]
EXISTENCE:physical interaction:GO:0032183 SUMO binding [PMID:17728242]
Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex; role in Ub-mediated degradation of histone variant Cse4p preventing mislocalization to euchromatin; role in proteolysis of spindle positioning protein Kar9p, and DNA repair proteins Rad52p and Rad57p; forms SUMO-dependent nuclear foci, including DNA repair centers; contains a RING domain and two SIM motifs; associates with the centromere; required for maintenance of genome integrity like human ortholog RNF4
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12514182]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:26928762|PMID:14562095]
EXISTENCE:direct assay:GO:0004362 glutathione-disulfide reductase (NADPH) activity [PMID:18400945]
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:18503006]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:18503006]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:18503006]
EXISTENCE:direct assay:GO:0005796 Golgi lumen [PMID:18400945]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:18400945]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18503006]
EXISTENCE:direct assay:GO:0051537 2 iron, 2 sulfur cluster binding [PMID:18171082]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:18400945]
EXISTENCE:mutant phenotype:GO:0004362 glutathione-disulfide reductase (NADPH) activity [PMID:18400945]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:18400945]
Cis-Golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
hypothetical protein; mRNA identified as translated by ribosome profiling data; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:10888670]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473]
Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
ARS415; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
proteasome regulatory particle base subunit RPT2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15210724]
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:9741626]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:7721833]
EXISTENCE:direct assay:GO:0045732 positive regulation of protein catabolic process [PMID:15135049]
EXISTENCE:genetic interaction:GO:0036402 proteasome-activating activity [PMID:11430818]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:9724628]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:23102099]
EXISTENCE:mutant phenotype:GO:0036402 proteasome-activating activity [PMID:11430818]
EXISTENCE:mutant phenotype:GO:0043171 peptide catabolic process [PMID:9724628]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19412160]
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:26546002|PMID:10580002]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11113180]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11113180]
EXISTENCE:direct assay:GO:1990439 MAP kinase serine/threonine phosphatase activity [PMID:11113180]
EXISTENCE:genetic interaction:GO:0043409 negative regulation of MAPK cascade [PMID:26546002]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:9529388]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:20400943]
EXISTENCE:mutant phenotype:GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation [PMID:8196609]
EXISTENCE:mutant phenotype:GO:0043409 negative regulation of MAPK cascade [PMID:26546002]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:14685261]
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating the osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation; involved in myosin-V dependent inheritance of mitochondria, peroxisomes, vacuoles and secretory vesicles; localizes to the nucleus, cytoplasm and peroxisomes
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:9891034]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12468546]
EXISTENCE:direct assay:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12482986]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15254252]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:33045248]
EXISTENCE:mutant phenotype:GO:0090399 replicative senescence [PMID:33045248]
EXISTENCE:physical interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11470794]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11470794]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:17082766]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18722382]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:8026496]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:8026496]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:8026496]
Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:18614046]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18614046|PMID:9990856]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:18321989]
EXISTENCE:direct assay:GO:0030892 mitotic cohesin complex [PMID:9990856]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:27325700]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11448778|PMID:23178808]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:18321989]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:18614046]
EXISTENCE:mutant phenotype:GO:0006979 response to oxidative stress [PMID:34897432]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:9335334]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:16962805]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:16888651]
EXISTENCE:physical interaction:GO:0019901 protein kinase binding [PMID:27325700]
Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separase Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:19726587]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12887900]
EXISTENCE:direct assay:GO:0031011 Ino80 complex [PMID:12887900]
EXISTENCE:direct assay:GO:0045027 DNA end binding [PMID:19726587]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:15607974]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:17762868]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:24034245]
Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins
Cytoplasmic protein required for sporulation; targeted to vacuole via AP-3 pathway
EXISTENCE:mutant phenotype:GO:0005993 trehalose catabolic process [PMID:23597844]
EXISTENCE:mutant phenotype:GO:0071465 cellular response to desiccation [PMID:25456447]
Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; phosphorylated and activated by Cdc28p at the G1/S phase transition to coordinately regulate carbohydrate metabolism and the cell cycle; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10825193]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10825193]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:7836422]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:10991951]
EXISTENCE:mutant phenotype:GO:0006405 RNA export from nucleus [PMID:7489726]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10991951]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:7489726]
Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:17287526|PMID:14562095|PMID:32421152]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:32421152]
EXISTENCE:direct assay:GO:0031982 vesicle [PMID:17287526]
EXISTENCE:direct assay:GO:0072665 protein localization to vacuole [PMID:32421152]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17287526]
Vacuolar ubiquitin ligase-substrate adaptor; delivered to vacuole using ubiquitin as a sorting signal; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:9159392]
EXISTENCE:direct assay:GO:0033065 Rad51C-XRCC3 complex [PMID:9159392]
EXISTENCE:mutant phenotype:GO:0000707 meiotic DNA recombinase assembly [PMID:9679065]
EXISTENCE:physical interaction:GO:0000730 DNA recombinase assembly [PMID:11459983]
Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
RNA polymerase III-inhibiting protein MAF1
EXISTENCE:direct assay:GO:0000994 RNA polymerase III core binding [PMID:16762835|PMID:20887893]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19574957|PMID:16762835|PMID:19684113]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:19574957]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16762835|PMID:19574957|PMID:19684113]
EXISTENCE:direct assay:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:16762835]
EXISTENCE:genetic interaction:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:11438659]
EXISTENCE:mutant phenotype:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:11438659|PMID:16762835]
EXISTENCE:physical interaction:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:11438659|PMID:16762835]
Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8065298]
EXISTENCE:genetic interaction:GO:0010737 protein kinase A signaling [PMID:8065298]
hypothetical protein; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
phosphoribosylanthranilate isomerase TRP1
EXISTENCE:direct assay:GO:0000162 tryptophan biosynthetic process [PMID:3286643]
EXISTENCE:direct assay:GO:0004640 phosphoribosylanthranilate isomerase activity [PMID:3286643]
EXISTENCE:mutant phenotype:GO:0000162 tryptophan biosynthetic process [PMID:348687|PMID:10790693]
EXISTENCE:mutant phenotype:GO:0004640 phosphoribosylanthranilate isomerase activity [PMID:348687]
Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA); enhances vegetative growth at low and high temperatures when used as an auxotrophic marker in strains such as W303
ARS1; Early-firing autonomously replicating sequence; highly efficient chromosomal replicator active in nearly every cell cycle
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18952899]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10866670|PMID:18952899]
EXISTENCE:mutant phenotype:GO:0000435 positive regulation of transcription from RNA polymerase II promoter by galactose [PMID:3013721]
EXISTENCE:mutant phenotype:GO:0006012 galactose metabolic process [PMID:4577139]
EXISTENCE:mutant phenotype:GO:0045185 maintenance of protein location [PMID:19651897]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:8628318]
Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12469340]
EXISTENCE:genetic interaction:GO:0030003 cellular cation homeostasis [PMID:8985171]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:8985171]
Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:22096102]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:22096102]
Ribosomal 60S subunit protein L4B; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4B has a paralog, RPL4A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:genetic interaction:GO:0031261 DNA replication preinitiation complex [PMID:12730134]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:12768207|PMID:12730134]
EXISTENCE:mutant phenotype:GO:0031261 DNA replication preinitiation complex [PMID:12730134]
EXISTENCE:physical interaction:GO:0000811 GINS complex [PMID:12768207|PMID:12730134]
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:18653893]
EXISTENCE:genetic interaction:GO:0034183 negative regulation of maintenance of mitotic sister chromatid cohesion [PMID:22901742]
EXISTENCE:mutant phenotype:GO:0034183 negative regulation of maintenance of mitotic sister chromatid cohesion [PMID:22901742|PMID:23219725]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:31617566]
EXISTENCE:mutant phenotype:GO:0140588 chromatin looping [PMID:32032532]
EXISTENCE:mutant phenotype:GO:1902340 negative regulation of chromosome condensation [PMID:23219725]
Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:16818607]
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:18347097]
EXISTENCE:direct assay:GO:0045950 negative regulation of mitotic recombination [PMID:16818607]
EXISTENCE:direct assay:GO:1903873 negative regulation of DNA recombinase mediator complex assembly [PMID:18347097]
EXISTENCE:genetic interaction:GO:0007130 synaptonemal complex assembly [PMID:24367271]
EXISTENCE:genetic interaction:GO:0045950 negative regulation of mitotic recombination [PMID:16818607]
EXISTENCE:mutant phenotype:GO:0004857 enzyme inhibitor activity [PMID:27483004]
EXISTENCE:mutant phenotype:GO:0045950 negative regulation of mitotic recombination [PMID:16818607]
Meiosis-specific protein; down-regulates Rad51p-mediated mitotic recombination when the meiotic recombination machinery is impaired; promotes synapsis and required for the normal morphogenesis of synaptonemal complex; prevents the recruitment of Rad54p to site-specific DNA double-strand breaks in vivo; early meiotic gene, transcribed specifically during meiotic prophase
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:15664196|PMID:16777964]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:17620411]
EXISTENCE:direct assay:GO:0031116 positive regulation of microtubule polymerization [PMID:15664196]
EXISTENCE:direct assay:GO:0042729 DASH complex [PMID:15664196|PMID:15640796]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:17620411|PMID:20479465]
EXISTENCE:direct assay:GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore [PMID:20479465|PMID:20479468]
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
EXISTENCE:direct assay:GO:0000827 inositol-1,3,4,5,6-pentakisphosphate kinase activity [PMID:10827188]
EXISTENCE:direct assay:GO:0000828 inositol hexakisphosphate kinase activity [PMID:10574768|PMID:11956213]
EXISTENCE:direct assay:GO:0000829 inositol heptakisphosphate kinase activity [PMID:11956213]
EXISTENCE:direct assay:GO:0032958 inositol phosphate biosynthetic process [PMID:10574768|PMID:17412958]
EXISTENCE:mutant phenotype:GO:0010919 regulation of inositol phosphate biosynthetic process [PMID:23824185]
EXISTENCE:mutant phenotype:GO:0032958 inositol phosphate biosynthetic process [PMID:11956213]
Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24390141]
Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication
ARS417; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0006730 one-carbon metabolic process [PMID:10871621]
EXISTENCE:mutant phenotype:GO:0004375 glycine dehydrogenase (decarboxylating) activity [PMID:9047339]
EXISTENCE:mutant phenotype:GO:0005960 glycine cleavage complex [PMID:9047339]
EXISTENCE:mutant phenotype:GO:0006544 glycine metabolic process [PMID:9047339]
EXISTENCE:mutant phenotype:GO:0006730 one-carbon metabolic process [PMID:10871621]
T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
hypothetical protein; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9372960]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16449634]
EXISTENCE:physical interaction:GO:0097078 FAL1-SGD1 complex [PMID:21576267]
Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:17362880|PMID:18497569]
EXISTENCE:direct assay:GO:1990316 Atg1/ULK1 kinase complex [PMID:26753620]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:17362880]
Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0004828 serine-tRNA ligase activity [PMID:3031581]
EXISTENCE:direct assay:GO:0006434 seryl-tRNA aminoacylation [PMID:3031581]
Cytosolic seryl-tRNA synthetase; class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:8070651]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:25092870]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748|PMID:14562095]
EXISTENCE:direct assay:GO:0006363 termination of RNA polymerase I transcription [PMID:22805593]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:22362748]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:22362748]
EXISTENCE:mutant phenotype:GO:0006363 termination of RNA polymerase I transcription [PMID:22805593]
RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:10637310]
EXISTENCE:direct assay:GO:0042147 retrograde transport, endosome to Golgi [PMID:12686613]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:10637310]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:17645731]
EXISTENCE:mutant phenotype:GO:0090156 cellular sphingolipid homeostasis [PMID:26357016]
EXISTENCE:physical interaction:GO:0000938 GARP complex [PMID:10637310]
Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10454550]
EXISTENCE:direct assay:GO:0007039 protein catabolic process in the vacuole [PMID:14684743]
EXISTENCE:mutant phenotype:GO:0006986 response to unfolded protein [PMID:25730376]
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:14684743]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:19723765]
EXISTENCE:mutant phenotype:GO:0043433 negative regulation of DNA-binding transcription factor activity [PMID:12167649]
Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YDR029W is not an essential gene
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:8830695]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:8830695]
Protein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:17095012]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:19703468]
Mitochondrial intermembrane space hypothetical protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003955 NAD(P)H dehydrogenase (quinone) activity [PMID:28499769]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
FMN-dependent NAD(P)H:quinone oxidoreductase; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
ARS450; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10675524|PMID:12469340|PMID:16622836]
Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; null mutation confers sensitivity to acetic acid
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9701810]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10975256]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11952910|PMID:7935367]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:3123231]
EXISTENCE:mutant phenotype:GO:2001196 positive regulation of lysine biosynthetic process via alpha-aminoadipate and saccharopine [PMID:3123231]
Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
YDRCTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
hypothetical protein; contained within the solo Ty1 LTR element YDRWdelta7
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
Predicted tail-anchored plasma membrane protein; contains conserved CYSTM module; related proteins in other organisms may be involved in response to stress; N- and C-terminal fusion proteins localize to the cell periphery; YDR034W-B has a paralog, YBR056W-A, that arose from the whole genome duplication
3-deoxy-7-phosphoheptulonate synthase ARO3
EXISTENCE:direct assay:GO:0003849 3-deoxy-7-phosphoheptulonate synthase activity [PMID:2880280]
3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:28154081]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:28154081]
EXISTENCE:direct assay:GO:0003860 3-hydroxyisobutyryl-CoA hydrolase activity [PMID:12697341]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:28154081]
3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
EXISTENCE:direct assay:GO:0004824 lysine-tRNA ligase activity [PMID:3888626]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:330225]
EXISTENCE:direct assay:GO:0006430 lysyl-tRNA aminoacylation [PMID:3888626]
EXISTENCE:mutant phenotype:GO:0004824 lysine-tRNA ligase activity [PMID:7628447]
EXISTENCE:mutant phenotype:GO:0006430 lysyl-tRNA aminoacylation [PMID:7628447]
putative Na(+)-exporting P-type ATPase ENA5
Protein with similarity to P-type ATPase sodium pumps; member of the Na+ efflux ATPase family
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7664728]
EXISTENCE:genetic interaction:GO:0006814 sodium ion transport [PMID:10073572|PMID:1657642]
EXISTENCE:genetic interaction:GO:0008554 P-type sodium transporter activity [PMID:1657642]
EXISTENCE:genetic interaction:GO:0015662 P-type ion transporter activity [PMID:1657642]
EXISTENCE:mutant phenotype:GO:0006813 potassium ion transport [PMID:11932440]
EXISTENCE:mutant phenotype:GO:0008556 P-type potassium transmembrane transporter activity [PMID:11932440]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:11932440]
P-type ATPase sodium pump; involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux
Na(+)/Li(+)-exporting P-type ATPase ENA1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:7664728|PMID:9315618|PMID:16622836]
EXISTENCE:direct assay:GO:0008554 P-type sodium transporter activity [PMID:9315618]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:9315618]
EXISTENCE:genetic interaction:GO:0006814 sodium ion transport [PMID:1657642]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:8612770]
EXISTENCE:genetic interaction:GO:0009268 response to pH [PMID:12453218]
EXISTENCE:genetic interaction:GO:0009651 response to salt stress [PMID:11457832]
EXISTENCE:mutant phenotype:GO:0042149 cellular response to glucose starvation [PMID:9383192]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9315618]
P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance
mitochondrial 37S ribosomal protein RSM10
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
hypothetical protein; expression is increased in ssu72-ts69 mutant
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position A2220 and small subunit (SSU) rRNA at position A619
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:17023428|PMID:10022891]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10022891]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11404322]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15466424|PMID:16278455|PMID:12024013]
EXISTENCE:genetic interaction:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:16278455]
EXISTENCE:genetic interaction:GO:0043709 cell adhesion involved in single-species biofilm formation [PMID:12024013]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:12024013]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18287536|PMID:12509465|PMID:17023428]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:11281938|PMID:11404322|PMID:10022891]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:10022891]
EXISTENCE:mutant phenotype:GO:0071475 cellular hyperosmotic salinity response [PMID:12509465]
EXISTENCE:mutant phenotype:GO:0090606 single-species surface biofilm formation [PMID:16556982]
EXISTENCE:physical interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12509465]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:10022891]
EXISTENCE:physical interaction:GO:0071475 cellular hyperosmotic salinity response [PMID:12509465]
Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose
EXISTENCE:direct assay:GO:0004109 coproporphyrinogen oxidase activity [PMID:3516695]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:3516695]
EXISTENCE:mutant phenotype:GO:0004109 coproporphyrinogen oxidase activity [PMID:2838478]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:7035824]
Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
DNA-directed RNA polymerase III core subunit RPC11
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793|PMID:9869639]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793|PMID:9869639]
EXISTENCE:mutant phenotype:GO:0001056 RNA polymerase III activity [PMID:9869639]
EXISTENCE:mutant phenotype:GO:0006386 termination of RNA polymerase III transcription [PMID:9869639]
EXISTENCE:mutant phenotype:GO:0042797 tRNA transcription by RNA polymerase III [PMID:9869639]
RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
EXISTENCE:direct assay:GO:0006865 amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0015171 amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10654085]
Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004853 uroporphyrinogen decarboxylase activity [PMID:2180703]
EXISTENCE:mutant phenotype:GO:0004853 uroporphyrinogen decarboxylase activity [PMID:7035824]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:1471989|PMID:14559249|PMID:7035824]
Uroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
EXISTENCE:direct assay:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:29632312]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:21070972|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:21148305]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:21070972]
EXISTENCE:direct assay:GO:0005840 ribosome [PMID:29632312]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:21070972]
Peptidyl-tRNA hydrolase that releases stalled peptides from ribosomes, component of a Cdc48p-complex; involved in protein quality control; exhibits cytosolic and ER-membrane localization; contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; translocates to mitochondria under oxidative stress and forms a complex with Cdc48p and Npl4p that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD)
ARS418; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004807 triose-phosphate isomerase activity [PMID:236471]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:27385335|PMID:11914276]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:11329176]
Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0003993 acid phosphatase activity [PMID:19753119]
EXISTENCE:mutant phenotype:GO:0032366 intracellular sterol transport [PMID:19060182]
Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
protein serine/threonine kinase activating protein DBF4
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:8066465]
EXISTENCE:direct assay:GO:0045860 positive regulation of protein kinase activity [PMID:19692334]
EXISTENCE:genetic interaction:GO:0001100 negative regulation of exit from mitosis [PMID:19478884]
EXISTENCE:genetic interaction:GO:0043539 protein serine/threonine kinase activator activity [PMID:8474449]
EXISTENCE:genetic interaction:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:8474449]
EXISTENCE:genetic interaction:GO:1903468 positive regulation of DNA replication initiation [PMID:9407029]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:19478884]
EXISTENCE:mutant phenotype:GO:0043539 protein serine/threonine kinase activator activity [PMID:8474449]
EXISTENCE:mutant phenotype:GO:0060903 positive regulation of meiosis I [PMID:16319063]
EXISTENCE:mutant phenotype:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:8474449]
EXISTENCE:physical interaction:GO:0006270 DNA replication initiation [PMID:8474449]
Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation
SCF E2 ubiquitin-protein ligase catalytic subunit CDC34
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:1848239]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:2842867|PMID:9346239]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155|PMID:8164658]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11080155]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238]
EXISTENCE:direct assay:GO:0051865 protein autoubiquitination [PMID:8383676]
EXISTENCE:genetic interaction:GO:0000086 G2/M transition of mitotic cell cycle [PMID:7954792|PMID:9736614]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:2842867]
EXISTENCE:mutant phenotype:GO:0019005 SCF ubiquitin ligase complex [PMID:9736614]
EXISTENCE:mutant phenotype:GO:0051865 protein autoubiquitination [PMID:8383676]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238]
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15583168]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953|PMID:15583168]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:15583168]
Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:14562095]
hypothetical protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:16168371]
EXISTENCE:direct assay:GO:0034099 luminal surveillance complex [PMID:16873065]
EXISTENCE:direct assay:GO:0070492 oligosaccharide binding [PMID:19111666]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16168370|PMID:16168371|PMID:16168372|PMID:22298424]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:22298424]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:24292014]
EXISTENCE:mutant phenotype:GO:0097466 ubiquitin-dependent glycoprotein ERAD pathway [PMID:19124653]
EXISTENCE:physical interaction:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
ARS419; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:9544243]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19959834]
EXISTENCE:mutant phenotype:GO:0004806 triglyceride lipase activity [PMID:9544243]
EXISTENCE:mutant phenotype:GO:0019433 triglyceride catabolic process [PMID:19959834]
Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA), decodes AGA codons into arginine, one of 19 nuclear tRNAs for arginine
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:2154373]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:2154373]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:10848595]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:29519818]
Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
RNA-binding ribosome biosynthesis protein MAK21
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11371346]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23209026]
EXISTENCE:direct assay:GO:0030690 Noc1p-Noc2p complex [PMID:11371346]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:9786894]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:23209026|PMID:11371346]
Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239|PMID:16823961]
Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:mutant phenotype:GO:0004758 serine C-palmitoyltransferase activity [PMID:1556076]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:1556075|PMID:1556076]
Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20362448|PMID:14562095]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:20362448]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:20362448]
EXISTENCE:direct assay:GO:0071846 actin filament debranching [PMID:20362448]
Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:2517480|PMID:3533916|PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2517480|PMID:3533916|PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:2517480|PMID:3533916|PMID:6814480]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:2517480]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
EXISTENCE:direct assay:GO:0099617 matrix side of mitochondrial inner membrane [PMID:30759361]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:19751518]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:16168084]
EXISTENCE:mutant phenotype:GO:0097745 mitochondrial tRNA 5'-end processing [PMID:30759361]
hypothetical protein; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; required for vacuolar acidification; localizes to the matrix side of the inner mitochondrial membrane
putative protein-serine/threonine phosphatase
hypothetical protein; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0000502 proteasome complex [PMID:10069815]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:19410548|PMID:8247125]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:17145966]
EXISTENCE:direct assay:GO:0010995 free ubiquitin chain depolymerization [PMID:19410548]
EXISTENCE:genetic interaction:GO:0070676 intralumenal vesicle formation [PMID:23444383]
EXISTENCE:mutant phenotype:GO:0004843 cysteine-type deubiquitinase activity [PMID:8247125]
EXISTENCE:mutant phenotype:GO:0006275 regulation of DNA replication [PMID:8657109]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:17145966]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:9119204]
EXISTENCE:mutant phenotype:GO:0010992 ubiquitin recycling [PMID:19410548]
EXISTENCE:mutant phenotype:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:17376168]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
hypothetical protein; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004059 aralkylamine N-acetyltransferase activity [PMID:11559708]
EXISTENCE:direct assay:GO:0004145 diamine N-acetyltransferase activity [PMID:15723835]
EXISTENCE:mutant phenotype:GO:0004145 diamine N-acetyltransferase activity [PMID:15723835]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:15723835]
Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
EXISTENCE:direct assay:GO:0006676 mannosyl diphosphorylinositol ceramide metabolic process [PMID:9368028]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:6991492]
EXISTENCE:direct assay:GO:0016772 transferase activity, transferring phosphorus-containing groups [PMID:9368028]
EXISTENCE:genetic interaction:GO:0010507 negative regulation of autophagy [PMID:20030721]
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:15792805]
Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:7623818]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17573544]
Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
EXISTENCE:mutant phenotype:GO:0004805 trehalose-phosphatase activity [PMID:8444170|PMID:9837904]
EXISTENCE:mutant phenotype:GO:0005992 trehalose biosynthetic process [PMID:8203161|PMID:8444170]
EXISTENCE:mutant phenotype:GO:0010508 positive regulation of autophagy [PMID:32240040]
EXISTENCE:mutant phenotype:GO:0070413 trehalose metabolism in response to stress [PMID:9837904]
EXISTENCE:physical interaction:GO:0005946 alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [PMID:9837904]
Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in the synthesis of storage carbohydrate trehalose; positive regulator of autophagy involved in the dephosphorylation of Rim15p leading to ATG8 induction; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
phosphoprotein phosphatase PP4 catalytic subunit PPH3
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:20951350]
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:16299494|PMID:7941742]
EXISTENCE:direct assay:GO:0030289 protein phosphatase 4 complex [PMID:16299494|PMID:17517611]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:21135129]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:16299494]
EXISTENCE:mutant phenotype:GO:0030289 protein phosphatase 4 complex [PMID:16299494]
EXISTENCE:mutant phenotype:GO:0051173 positive regulation of nitrogen compound metabolic process [PMID:10940301]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:20951350]
EXISTENCE:mutant phenotype:GO:1902660 negative regulation of glucose mediated signaling pathway [PMID:24277933]
EXISTENCE:mutant phenotype:GO:2000002 negative regulation of DNA damage checkpoint [PMID:16299494|PMID:17517611]
EXISTENCE:mutant phenotype:GO:2001034 positive regulation of double-strand break repair via nonhomologous end joining [PMID:24498054]
Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
EXISTENCE:mutant phenotype:GO:0000730 DNA recombinase assembly [PMID:11459983|PMID:12887906|PMID:14765116]
Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15351639]
EXISTENCE:direct assay:GO:0005840 ribosome [PMID:12627396]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9613572]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:15351639]
EXISTENCE:mutant phenotype:GO:0005199 structural constituent of cell wall [PMID:9642191]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9642191]
Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0070987 error-free translesion synthesis [PMID:19496932]
EXISTENCE:direct assay:GO:0097196 Shu complex [PMID:23575680]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:15654096]
EXISTENCE:genetic interaction:GO:0043007 maintenance of rDNA [PMID:21372173]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0000730 DNA recombinase assembly [PMID:23575680]
EXISTENCE:mutant phenotype:GO:0035861 site of double-strand break [PMID:23575680]
EXISTENCE:physical interaction:GO:0097196 Shu complex [PMID:15654096]
Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; important for both mitotic and meiotic homologous recombination, and contains a conserved SWIM domain that is necessary for both
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:15507444|PMID:11498004]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:11498004]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:11498004|PMID:15507444]
Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
EXISTENCE:curator inference:GO:0000993 RNA polymerase II complex binding [PMID:19818408]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22932476]
EXISTENCE:direct assay:GO:0005675 transcription factor TFIIH holo complex [PMID:15220919|PMID:27381459]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:30905750]
EXISTENCE:direct assay:GO:0070816 phosphorylation of RNA polymerase II C-terminal domain [PMID:19450536|PMID:19679665]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:17215295]
EXISTENCE:physical interaction:GO:0000439 transcription factor TFIIH core complex [PMID:17215295]
Component of RNA polymerase II general transcription factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; relocalizes to the cytosol in response to hypoxia; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:9111041|PMID:26928762]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:9159129]
EXISTENCE:mutant phenotype:GO:0015031 protein transport [PMID:9184222]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:18809726|PMID:20604902|PMID:21325627]
Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:22404710]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:22404710]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:19013460]
EXISTENCE:genetic interaction:GO:0090180 positive regulation of thiamine biosynthetic process [PMID:16850348]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8264540]
EXISTENCE:mutant phenotype:GO:0090180 positive regulation of thiamine biosynthetic process [PMID:16850348]
EXISTENCE:mutant phenotype:GO:2001172 positive regulation of glycolytic fermentation to ethanol [PMID:8264540]
Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17293872]
EXISTENCE:genetic interaction:GO:0016233 telomere capping [PMID:15132993|PMID:9042864]
EXISTENCE:genetic interaction:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:17947422]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:9042864|PMID:15132993]
EXISTENCE:mutant phenotype:GO:0032211 negative regulation of telomere maintenance via telomerase [PMID:17947422]
EXISTENCE:physical interaction:GO:0016233 telomere capping [PMID:15132993|PMID:9042864]
EXISTENCE:physical interaction:GO:1990879 CST complex [PMID:11230140|PMID:9042864]
Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1
25S rRNA (adenine645-N1)-methyltransferase
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10864042|PMID:23180764|PMID:22588997]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:22588997]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:10864042]
EXISTENCE:genetic interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:23180764]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:10864042]
EXISTENCE:mutant phenotype:GO:0016433 rRNA (adenine) methyltransferase activity [PMID:23180764]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:23180764]
Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with gar1 mutation; deletion disrupts telomere maintenance by influencing expression of neighboring gene STN1; mammalian homolog nucleomethylin has been linked to nutrient availability signaling pathways and obesity
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:16107716]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17178117]
Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:7823940]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:18552279|PMID:8413281]
EXISTENCE:mutant phenotype:GO:0032185 septin cytoskeleton organization [PMID:18552279]
EXISTENCE:mutant phenotype:GO:0097271 protein localization to bud neck [PMID:18552279]
Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; also detected in peroxisomes; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:27831485]
EXISTENCE:direct assay:GO:0005784 Sec61 translocon complex [PMID:7758110|PMID:8612571]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0015450 protein-transporting ATPase activity [PMID:7758110]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:direct assay:GO:0071261 Ssh1 translocon complex [PMID:8612571]
EXISTENCE:genetic interaction:GO:0005198 structural molecule activity [PMID:7961662]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:7961662]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:12860997]
Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p); this complex forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614|PMID:17443350]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:3549696]
Essential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles
EXISTENCE:direct assay:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:12212850]
EXISTENCE:direct assay:GO:0000386 second spliceosomal transesterification activity [PMID:12212850]
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:12212850]
EXISTENCE:direct assay:GO:0071021 U2-type post-spliceosomal complex [PMID:12212850]
RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 6 nuclear tRNA genes containing the tDNA-anticodon ACG (converted to ICG in the mature tRNA), decodes CGU, CGC, and probably CGA codons into arginine, one of 19 nuclear tRNAs for arginine
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:27587415|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0006799 polyphosphate biosynthetic process [PMID:27587415]
EXISTENCE:mutant phenotype:GO:0030643 cellular phosphate ion homeostasis [PMID:27587415]
EXISTENCE:physical interaction:GO:0033254 vacuolar transporter chaperone complex [PMID:27587415]
Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:30045857]
hypothetical protein; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane
Fe-S cluster-binding ribosome biosynthesis protein
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:15660134]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15277527]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15277527|PMID:15660134|PMID:14562095]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:15277527]
EXISTENCE:direct assay:GO:0032790 ribosome disassembly [PMID:24424461]
EXISTENCE:genetic interaction:GO:0006415 translational termination [PMID:20062004]
EXISTENCE:mutant phenotype:GO:0000054 ribosomal subunit export from nucleus [PMID:15660134|PMID:22855532]
EXISTENCE:mutant phenotype:GO:0006413 translational initiation [PMID:15277527|PMID:15660134]
EXISTENCE:mutant phenotype:GO:0032790 ribosome disassembly [PMID:22770215]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:15277527]
EXISTENCE:mutant phenotype:GO:0045727 positive regulation of translation [PMID:24424461]
EXISTENCE:physical interaction:GO:0006415 translational termination [PMID:20062004]
Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress
ARS420; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:10089880]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:32265276]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:10089880]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10880451]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10880451]
EXISTENCE:direct assay:GO:0010994 free ubiquitin chain polymerization [PMID:19706603]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:10089880]
EXISTENCE:mutant phenotype:GO:0006301 postreplication repair [PMID:10089880]
EXISTENCE:mutant phenotype:GO:0044395 protein targeting to vacuolar membrane [PMID:32265276]
EXISTENCE:physical interaction:GO:0031371 ubiquitin conjugating enzyme complex [PMID:10089880]
E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
aminophospholipid-translocating P4-type ATPase DNF2
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12221123|PMID:12631737|PMID:33060204]
EXISTENCE:direct assay:GO:0070867 mating projection tip membrane [PMID:25378585]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:12631737]
EXISTENCE:genetic interaction:GO:0007163 establishment or maintenance of cell polarity [PMID:15461661]
EXISTENCE:genetic interaction:GO:0015247 aminophospholipid flippase activity [PMID:12631737|PMID:17015438]
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:12631737|PMID:17015438]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:12631737]
EXISTENCE:mutant phenotype:GO:0015247 aminophospholipid flippase activity [PMID:12631737|PMID:17015438]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:12631737|PMID:17015438]
EXISTENCE:mutant phenotype:GO:0099040 ceramide translocation [PMID:33060204]
EXISTENCE:mutant phenotype:GO:0140346 phosphatidylserine flippase activity [PMID:12631737]
EXISTENCE:mutant phenotype:GO:0140351 glycosylceramide flippase activity [PMID:33060204]
Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10835355|PMID:12799368]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12799368]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:12799368]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10835355]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10523651|PMID:24140345]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:12799368]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10835355]
EXISTENCE:mutant phenotype:GO:0051864 histone H3K36 demethylase activity [PMID:17369256]
EXISTENCE:mutant phenotype:GO:0071071 regulation of phospholipid biosynthetic process [PMID:12799368]
Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
ARS421; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:10066781]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:9545323]
EXISTENCE:direct assay:GO:0006298 mismatch repair [PMID:11237611]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:9819445]
EXISTENCE:direct assay:GO:0032137 guanine/thymine mispair binding [PMID:8816473]
EXISTENCE:direct assay:GO:0032138 single base insertion or deletion binding [PMID:8816473]
EXISTENCE:genetic interaction:GO:0036297 interstrand cross-link repair [PMID:22912599]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:16702432]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:2685551|PMID:9520271]
EXISTENCE:mutant phenotype:GO:0032138 single base insertion or deletion binding [PMID:10347163]
EXISTENCE:mutant phenotype:GO:0043111 replication fork arrest [PMID:27045900]
EXISTENCE:physical interaction:GO:0032301 MutSalpha complex [PMID:8816473]
Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p; contains PIP motif that binds PCNA (Pol30p) and Rev1p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15456753]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18281282]
EXISTENCE:direct assay:GO:0051536 iron-sulfur cluster binding [PMID:31493153]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:17074835]
EXISTENCE:mutant phenotype:GO:0015036 disulfide oxidoreductase activity [PMID:10567543|PMID:27085841]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:20889785]
EXISTENCE:mutant phenotype:GO:0031065 positive regulation of histone deacetylation [PMID:27085841]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17074835|PMID:10567543]
EXISTENCE:mutant phenotype:GO:2000678 negative regulation of transcription regulatory region DNA binding [PMID:23045394]
EXISTENCE:physical interaction:GO:0006879 cellular iron ion homeostasis [PMID:17074835]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:23045394]
Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive monothiol glutaredoxin subfamily member with Grx4p and Grx5p; redundantly protects cells from oxidative damage along with GRX4 and GRX5; with Grx4p, promotes the dissociation of Aft1p from iron regulon gene promoters and subsequent nuclear export in iron-replete conditions, regulating iron homeostasis; involved with Grx4p in the deglutathionylation of Sir2p, restoring deacetylase activity after disulfide stress
YDRCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:12167636]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12167636|PMID:25409301|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25409301|PMID:22842922]
EXISTENCE:genetic interaction:GO:0001402 signal transduction involved in filamentous growth [PMID:9215628]
EXISTENCE:genetic interaction:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:19934224]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:19934224]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:9215628]
EXISTENCE:genetic interaction:GO:0007265 Ras protein signal transduction [PMID:9215628]
EXISTENCE:genetic interaction:GO:0030437 ascospore formation [PMID:9215628]
EXISTENCE:genetic interaction:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:17559233]
EXISTENCE:genetic interaction:GO:0070873 regulation of glycogen metabolic process [PMID:9215628]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:14704161]
EXISTENCE:mutant phenotype:GO:0043066 negative regulation of apoptotic process [PMID:22785534]
EXISTENCE:mutant phenotype:GO:0050815 phosphoserine residue binding [PMID:9822578]
EXISTENCE:physical interaction:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:17030612]
14-3-3 protein, minor isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates multiple processes including exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; abundance relative to Bmh1p increases during sporulation; similar to several human 14-3-3 proteins
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:14562095]
Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16648468]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:16648468|PMID:12374754]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16648468]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16648468|PMID:12374754]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:16648468]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:17803941|PMID:18077551]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:16648468|PMID:18077551]
Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex
hypothetical protein; conserved among S. cerevisiae strains; YDR102C is not an essential gene; homozygous diploid deletion strain exhibits high budding index
EXISTENCE:direct assay:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8062390]
EXISTENCE:direct assay:GO:0001934 positive regulation of protein phosphorylation [PMID:19303851]
EXISTENCE:direct assay:GO:0005078 MAP-kinase scaffold activity [PMID:8062390]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:16847350]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9732267]
EXISTENCE:direct assay:GO:0070867 mating projection tip membrane [PMID:9732267]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:15192700]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:16847350]
EXISTENCE:mutant phenotype:GO:0043409 negative regulation of MAPK cascade [PMID:16424299]
EXISTENCE:physical interaction:GO:0005078 MAP-kinase scaffold activity [PMID:7851759]
EXISTENCE:physical interaction:GO:0031681 G-protein beta-subunit binding [PMID:7667635]
Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24036347]
EXISTENCE:direct assay:GO:1902657 protein localization to prospore membrane [PMID:24036347]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:22611022|PMID:26605945|PMID:27303688]
Meiosis-specific protein required for prospore membrane morphogenesis; localizes to the prospore membrane (PSM) during sporulation; required for PSM elongation and closure; genetically antagonistic to SPO1; recruits Vps13p to the PSM during sporulation; interacts and functions cooperatively with Spo73p; mutants have defects in the PSM, aberrant spore wall formation and reduced PtdIns-phosphate pools in the PSM; contains three PH-like domains
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:26928762|PMID:23836928]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
Vacuolar membrane hypothetical protein; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11011149]
EXISTENCE:genetic interaction:GO:0000278 mitotic cell cycle [PMID:16291862]
EXISTENCE:physical interaction:GO:0005200 structural constituent of cytoskeleton [PMID:16291862]
EXISTENCE:physical interaction:GO:0005869 dynactin complex [PMID:16291862]
Component of the dynactin complex; localized to the pointed end of the Arp1p filament; may regulate membrane association of the complex
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20681974]
EXISTENCE:genetic interaction:GO:0006878 cellular copper ion homeostasis [PMID:20681974]
EXISTENCE:genetic interaction:GO:0007034 vacuolar transport [PMID:20526336]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:18178563]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:18178563]
Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:20375281|PMID:27672095]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:23986483]
EXISTENCE:direct assay:GO:0031410 cytoplasmic vesicle [PMID:23129774]
EXISTENCE:direct assay:GO:1990072 TRAPPIII protein complex [PMID:20375281]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10727015]
EXISTENCE:genetic interaction:GO:0034497 protein localization to phagophore assembly site [PMID:27672095]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:23129774|PMID:16079147]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11239471]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:16079147|PMID:16874038|PMID:19793921|PMID:20375281]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:16079147|PMID:16874038]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:16874038|PMID:19793921|PMID:20375281]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:20375281|PMID:23129774|PMID:27672095]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8619313]
EXISTENCE:mutant phenotype:GO:0071255 Cvt vesicle assembly [PMID:16079147]
Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
EXISTENCE:direct assay:GO:0019150 D-ribulokinase activity [PMID:27909055]
EXISTENCE:direct assay:GO:0019321 pentose metabolic process [PMID:27909055]
EXISTENCE:mutant phenotype:GO:0019321 pentose metabolic process [PMID:27909055]
D-ribulokinase; carbohydrate kinase that specifically converts D-ribulose to D-ribulose 5-phosphate during pentose metabolism; null mutant accumulates D-ribulose and the precursor ribitol; proposed to function as a D-ribulose metabolic repair enzyme; member of the FGGY family of carbohydrate kinases; human homolog, FGGY, is involved in both D-ribulose and ribitol metabolism; human FGGY has been linked to both sporadic amyotrophic lateral sclerosis and bipolar disorder
replication fork barrier binding protein FOB1
EXISTENCE:direct assay:GO:0000183 rDNA heterochromatin formation [PMID:12923057]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10230397]
EXISTENCE:direct assay:GO:0008156 negative regulation of DNA replication [PMID:14645529]
EXISTENCE:direct assay:GO:0043110 rDNA spacer replication fork barrier binding [PMID:14645529]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:16769819]
EXISTENCE:genetic interaction:GO:0043007 maintenance of rDNA [PMID:23593017]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:26951198]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:9078378]
EXISTENCE:mutant phenotype:GO:0031582 replication fork arrest at rDNA repeats [PMID:26951198]
EXISTENCE:mutant phenotype:GO:0034503 protein localization to nucleolar rDNA repeats [PMID:19362534]
EXISTENCE:mutant phenotype:GO:0043110 rDNA spacer replication fork barrier binding [PMID:26951198]
EXISTENCE:mutant phenotype:GO:0043570 maintenance of DNA repeat elements [PMID:16507999]
EXISTENCE:mutant phenotype:GO:0045911 positive regulation of DNA recombination [PMID:25822194]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:18923139]
Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases
EXISTENCE:direct assay:GO:0030170 pyridoxal phosphate binding [PMID:29867852]
Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11149918|PMID:19520826]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19520826]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:11149918]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:15533838]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:9635435]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:15533838]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:27418100]
EXISTENCE:mutant phenotype:GO:0007127 meiosis I [PMID:19001291]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:11149918]
EXISTENCE:physical interaction:GO:0019899 enzyme binding [PMID:11149918|PMID:9635435]
Securin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
hypothetical protein; deletion mutant exhibits poor growth at elevated pH and calcium
putative mitochondrial 54S ribosomal protein
Putative mitochondrial ribosomal protein of the large subunit; required for respiratory growth and for efficient translation of mitochondrial COX1 and COX3; conditional mutant has reduced cytochrome c oxidase activity; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress; contains and N-terminal mitochondrial targeting sequence; similar to E. coli L34 ribosomal protein
mitochondrial 54S ribosomal protein MRPL1
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:14759368]
EXISTENCE:physical interaction:GO:0006364 rRNA processing [PMID:14759368]
hypothetical protein that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:26928762]
hypothetical protein; proposed role as basic amino acid permease based on phylogeny; targeted to vacuole via AP-3 pathway; physical interaction with Atg27p suggests possible role in autophagy; non-essential gene
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:22342701]
EXISTENCE:direct assay:GO:0005746 mitochondrial respirasome [PMID:27091403]
EXISTENCE:mutant phenotype:GO:0005751 mitochondrial respiratory chain complex IV [PMID:27091403]
Subunit of cytochrome C oxidase complex; stabilizes or regulates formation of respiratory chain supercomplexes composed of Complex III (ubiquinol-cytochrome c reductase) and Complex IV (cytochrome c oxidase)
EXISTENCE:direct assay:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:9801306]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:7599275]
EXISTENCE:direct assay:GO:0005637 nuclear inner membrane [PMID:7599275]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7599275]
EXISTENCE:mutant phenotype:GO:0004809 tRNA (guanine-N2-)-methyltransferase activity [PMID:9801306]
EXISTENCE:mutant phenotype:GO:0005637 nuclear inner membrane [PMID:19602197]
EXISTENCE:mutant phenotype:GO:0030488 tRNA methylation [PMID:2426253]
tRNA methyltransferase; two forms of protein are made by alternative translation starts; localizes to both nucleus and mitochondrion to produce modified base N2,N2-dimethylguanosine in tRNAs in both compartments; nuclear Trm1p is evenly distributed around inner nuclear membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body (SPB) mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon SPB
DNA polymerase epsilon noncatalytic subunit
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:14622139|PMID:16916794]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:14622139]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:14622139|PMID:16369485]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:19595716]
EXISTENCE:direct assay:GO:0008622 epsilon DNA polymerase complex [PMID:11024162|PMID:12571237]
EXISTENCE:direct assay:GO:0008623 CHRAC [PMID:14673157|PMID:15024052]
EXISTENCE:direct assay:GO:0030337 DNA polymerase processivity factor activity [PMID:16369485]
EXISTENCE:direct assay:GO:0031490 chromatin DNA binding [PMID:17491017]
EXISTENCE:direct assay:GO:0031492 nucleosomal DNA binding [PMID:19595716]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:19776424]
EXISTENCE:genetic interaction:GO:0006272 leading strand elongation [PMID:21124948]
EXISTENCE:genetic interaction:GO:0008310 single-stranded DNA 3'-5' exodeoxyribonuclease activity [PMID:21124948]
EXISTENCE:genetic interaction:GO:0030337 DNA polymerase processivity factor activity [PMID:21124948]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:11024162]
EXISTENCE:mutant phenotype:GO:0006272 leading strand elongation [PMID:21124948]
EXISTENCE:physical interaction:GO:0008622 epsilon DNA polymerase complex [PMID:15024052]
EXISTENCE:physical interaction:GO:0008623 CHRAC [PMID:15024052]
Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:1652871|PMID:8203145|PMID:30305396]
EXISTENCE:direct assay:GO:0009898 cytoplasmic side of plasma membrane [PMID:8020610]
EXISTENCE:genetic interaction:GO:0045921 positive regulation of exocytosis [PMID:15563607]
EXISTENCE:genetic interaction:GO:1903896 positive regulation of IRE1-mediated unfolded protein response [PMID:25348718]
EXISTENCE:physical interaction:GO:0005886 plasma membrane [PMID:15563607]
EXISTENCE:physical interaction:GO:0045921 positive regulation of exocytosis [PMID:15563607]
S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated unfolded protein response; regulates HAC1 mRNA translocation, splicing and translation with KIN2 during ER stress; direct phosphorylation of the substrate Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the plasma membrane; activation loop phosphorylation (T302) required for full kinase activity; orthologous to MARK/PAR-1, AMP-activated protein kinase (AMPK) family members
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:8195172]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8195172]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8012448|PMID:15455074]
EXISTENCE:direct assay:GO:0090575 RNA polymerase II transcription regulator complex [PMID:8195172]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8195172]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:8125958]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8125958]
EXISTENCE:physical interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8195172]
EXISTENCE:physical interaction:GO:0090575 RNA polymerase II transcription regulator complex [PMID:8195172]
Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:31945465]
Mitochondrial hypothetical protein; null mutant show severe fragmentation of mitochondria; expression is strongly induced by alpha factor; may have role in maintenance of mitochondrial morphology
hypothetical protein; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
ARS422; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:18701706]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:18701706]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:15973437]
EXISTENCE:genetic interaction:GO:0042144 vacuole fusion, non-autophagic [PMID:16186255]
EXISTENCE:mutant phenotype:GO:0016409 palmitoyltransferase activity [PMID:15973437|PMID:26224664]
EXISTENCE:mutant phenotype:GO:0017157 regulation of exocytosis [PMID:18701706]
EXISTENCE:mutant phenotype:GO:0018345 protein palmitoylation [PMID:15973437|PMID:16751107]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:18701706]
EXISTENCE:mutant phenotype:GO:0030866 cortical actin cytoskeleton organization [PMID:18701706]
Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
EXISTENCE:direct assay:GO:0003855 3-dehydroquinate dehydratase activity [PMID:6355828]
EXISTENCE:direct assay:GO:0003856 3-dehydroquinate synthase activity [PMID:6355828]
EXISTENCE:direct assay:GO:0003866 3-phosphoshikimate 1-carboxyvinyltransferase activity [PMID:6355828]
EXISTENCE:direct assay:GO:0004764 shikimate 3-dehydrogenase (NADP+) activity [PMID:6355828]
EXISTENCE:direct assay:GO:0004765 shikimate kinase activity [PMID:6355828]
Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:23716719]
Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:3060468]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:12857851]
EXISTENCE:direct assay:GO:0032432 actin filament bundle [PMID:3060468]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:2643162|PMID:3060468]
EXISTENCE:genetic interaction:GO:0070649 formin-nucleated actin cable assembly [PMID:27068241]
EXISTENCE:mutant phenotype:GO:0007015 actin filament organization [PMID:8522605]
EXISTENCE:mutant phenotype:GO:0070649 formin-nucleated actin cable assembly [PMID:27068241]
Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant
EXISTENCE:direct assay:GO:0000235 astral microtubule [PMID:17173039]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19948764]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:11929974]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11929974|PMID:17173039]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:17804403|PMID:11929974|PMID:17173039|PMID:22842922]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:17804403]
EXISTENCE:direct assay:GO:0005882 intermediate filament [PMID:11929974]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:17173039]
EXISTENCE:direct assay:GO:0045104 intermediate filament cytoskeleton organization [PMID:12551942]
EXISTENCE:direct assay:GO:0072686 mitotic spindle [PMID:11929974|PMID:17173039]
EXISTENCE:genetic interaction:GO:1902426 deactivation of mitotic spindle assembly checkpoint [PMID:19948764]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:17173039]
EXISTENCE:mutant phenotype:GO:0007052 mitotic spindle organization [PMID:17173039|PMID:17804403]
EXISTENCE:mutant phenotype:GO:0008017 microtubule binding [PMID:17804403]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19948764]
EXISTENCE:mutant phenotype:GO:1902426 deactivation of mitotic spindle assembly checkpoint [PMID:19948764]
EXISTENCE:physical interaction:GO:0000164 protein phosphatase type 1 complex [PMID:19948764]
Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:10582239|PMID:14747994]
EXISTENCE:direct assay:GO:0031625 ubiquitin protein ligase binding [PMID:21186364]
EXISTENCE:genetic interaction:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:10582239]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22842922]
Protein that associates with the large mitoribosomal subunit; abundance and relative nuclear distribution to the protein increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10383953]
Cell wall protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication; S. cerevisiae genome reference strain S288C contains an internal in-frame stop at codon 67, which in other strains encodes glutamine
ATP-binding cassette glutathione S-conjugate transporter YCF1
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23658021|PMID:8692889|PMID:26928762|PMID:23836928]
EXISTENCE:direct assay:GO:0010038 response to metal ion [PMID:10220408|PMID:12755704|PMID:9217966]
EXISTENCE:direct assay:GO:0015431 ABC-type glutathione S-conjugate transporter activity [PMID:10220408|PMID:12383937|PMID:12755704|PMID:9217966]
EXISTENCE:genetic interaction:GO:0006749 glutathione metabolic process [PMID:23242256]
EXISTENCE:mutant phenotype:GO:0015127 bilirubin transmembrane transporter activity [PMID:10790694]
EXISTENCE:mutant phenotype:GO:0015723 bilirubin transport [PMID:10790694]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:23658021]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:23242256]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:10790694]
Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10990452]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:10990452]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:10990452]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:11082047]
EXISTENCE:physical interaction:GO:0000139 Golgi membrane [PMID:10990452]
EXISTENCE:physical interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10990452]
EXISTENCE:physical interaction:GO:0034066 Ric1-Rgp1 guanyl-nucleotide exchange factor complex [PMID:10990452]
EXISTENCE:physical interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:10990452]
Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p
EXISTENCE:direct assay:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:24084588]
EXISTENCE:direct assay:GO:0003676 nucleic acid binding [PMID:12093753]
EXISTENCE:mutant phenotype:GO:0000445 THO complex part of transcription export complex [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0000446 nucleoplasmic THO complex [PMID:11060033]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:11060033|PMID:3044923]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:12093753|PMID:17960421]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:11979277]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:26663077]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:16908536]
EXISTENCE:mutant phenotype:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:26663077]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:9891041]
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
EXISTENCE:direct assay:GO:0031386 protein tag [PMID:9531531|PMID:9545234]
EXISTENCE:direct assay:GO:0045116 protein neddylation [PMID:9531531|PMID:9545234]
EXISTENCE:genetic interaction:GO:0045116 protein neddylation [PMID:9531531]
Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
S-adenosylmethionine-dependent methyltransferase
EXISTENCE:direct assay:GO:0006417 regulation of translation [PMID:15509572|PMID:16321977]
EXISTENCE:direct assay:GO:0008276 protein methyltransferase activity [PMID:15509572|PMID:17008308]
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:16321977]
EXISTENCE:direct assay:GO:0015934 large ribosomal subunit [PMID:33166396]
EXISTENCE:direct assay:GO:0018364 peptidyl-glutamine methylation [PMID:17008308|PMID:15509572]
EXISTENCE:mutant phenotype:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:16321977|PMID:33166396]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:33166396]
EXISTENCE:physical interaction:GO:0035657 eRF1 methyltransferase complex [PMID:17008308]
S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20477991]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16301316|PMID:30981741]
EXISTENCE:mutant phenotype:GO:0000301 retrograde transport, vesicle recycling within Golgi [PMID:30981741]
EXISTENCE:mutant phenotype:GO:0000902 cell morphogenesis [PMID:10931277]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:16301316]
Protein involved in vesicular transport at trans-Golgi network (TGN); TGN-localized, leucine-zipper domain protein; involved in endosome-to-Golgi transport during endocytic recycling, and retrograde transport of glycosyltransferases from the TGN to the Golgi; involved in organization of the ER, establishment of cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005053 peroxisome matrix targeting signal-2 binding [PMID:8670791]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:15545321|PMID:7535304|PMID:7957058]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15545321|PMID:7957058]
EXISTENCE:physical interaction:GO:0005053 peroxisome matrix targeting signal-2 binding [PMID:8636211]
EXISTENCE:physical interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:10087260|PMID:9094717]
Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:15797381|PMID:15078868]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15797381]
EXISTENCE:direct assay:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15078868|PMID:15797381]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:20080635]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15797381]
EXISTENCE:mutant phenotype:GO:0051788 response to misfolded protein [PMID:15797381]
EXISTENCE:mutant phenotype:GO:0071630 nuclear protein quality control by the ubiquitin-proteasome system [PMID:20080635]
Ubiquitin-protein ligase; role in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity; contains intrinsically disordered regions involved in substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins; localizes to the cytoplasm, nucleus and peroxisomes
EXISTENCE:direct assay:GO:0004190 aspartic-type endopeptidase activity [PMID:10446224]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:16087741]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:18591427]
GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity []
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10817999|PMID:12600943]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
EXISTENCE:direct assay:GO:0016573 histone acetylation [PMID:9674426]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:10751405]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
EXISTENCE:mutant phenotype:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:10751405|PMID:11561287|PMID:12582246]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12600943|PMID:12840001]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:9674426]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:12501245]
Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; overexpression of the human ortholog, TAF12, an oncogene involved in the formation of choroid plexus carcinomas, results in dosage chromosomal instability (dCIN) in a human cell line similar to the dCIN observed in yeast overexpressors
ARS451; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:3284746]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8816483]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8816483]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10409653]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:10319811|PMID:11562354]
EXISTENCE:mutant phenotype:GO:0007074 positive regulation of transcription involved in exit from mitosis, from RNA polymerase II promoter [PMID:9017392|PMID:9529390]
EXISTENCE:mutant phenotype:GO:0031496 positive regulation of mating type switching [PMID:6436497]
EXISTENCE:mutant phenotype:GO:0036033 mediator complex binding [PMID:11562354]
EXISTENCE:physical interaction:GO:0036033 mediator complex binding [PMID:11562354]
Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
bifunctional choline kinase/ethanolamine kinase EKI1
EXISTENCE:direct assay:GO:0004103 choline kinase activity [PMID:10329685]
EXISTENCE:direct assay:GO:0004305 ethanolamine kinase activity [PMID:10329685]
EXISTENCE:genetic interaction:GO:0004103 choline kinase activity [PMID:10329685]
EXISTENCE:genetic interaction:GO:0004305 ethanolamine kinase activity [PMID:10329685]
EXISTENCE:genetic interaction:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:10329685]
EXISTENCE:genetic interaction:GO:0006656 phosphatidylcholine biosynthetic process [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0004305 ethanolamine kinase activity [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0006646 phosphatidylethanolamine biosynthetic process [PMID:10329685]
EXISTENCE:mutant phenotype:GO:0006656 phosphatidylcholine biosynthetic process [PMID:10329685]
Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
ARS423; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0006099 tricarboxylic acid cycle [PMID:2072900]
EXISTENCE:direct assay:GO:0009353 mitochondrial oxoglutarate dehydrogenase complex [PMID:2072900]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431|PMID:11926067]
EXISTENCE:mutant phenotype:GO:0006103 2-oxoglutarate metabolic process [PMID:2115121]
Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:15023338]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:25083872]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11121446]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11121446]
EXISTENCE:direct assay:GO:0015631 tubulin binding [PMID:11266443]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:17336903]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:23341591|PMID:26839174]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:23641071]
EXISTENCE:mutant phenotype:GO:0000226 microtubule cytoskeleton organization [PMID:11121446]
EXISTENCE:mutant phenotype:GO:0000266 mitochondrial fission [PMID:17336903]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:11121446]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:20505073|PMID:23341591|PMID:26839174]
Protein required for nuclear migration; component of the mitochondria-ER-cortex-anchor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:15652485]
EXISTENCE:genetic interaction:GO:0000956 nuclear-transcribed mRNA catabolic process [PMID:15652485]
EXISTENCE:genetic interaction:GO:0006879 cellular iron ion homeostasis [PMID:15652485]
mRNA binding protein, member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; functions with Tis11p in regulation of iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005844 polysome [PMID:19448108]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:21076151]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:23899355]
EXISTENCE:mutant phenotype:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:23899355]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:23899355]
Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsically unstructured protein whose stability is enhanced upon binding Rbg2p
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15107463]
EXISTENCE:direct assay:GO:0030125 clathrin vesicle coat [PMID:12483220]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:12483220]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:15107463]
EXISTENCE:genetic interaction:GO:0034498 early endosome to Golgi transport [PMID:20658963]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:12483220]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:15107463]
EXISTENCE:mutant phenotype:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:15107463]
Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:2687115]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15643056]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:genetic interaction:GO:0045836 positive regulation of meiotic nuclear division [PMID:15643056]
EXISTENCE:mutant phenotype:GO:0016018 cyclosporin A binding [PMID:7515500]
EXISTENCE:mutant phenotype:GO:0019538 protein metabolic process [PMID:11641409|PMID:12242280]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:15643056]
EXISTENCE:mutant phenotype:GO:0045836 positive regulation of meiotic nuclear division [PMID:15643056]
EXISTENCE:physical interaction:GO:0034967 Set3 complex [PMID:15643056]
Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress
DNA-directed RNA polymerase I subunit RPA14
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:7768955|PMID:9837969]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:genetic interaction:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:9121426]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7768955]
EXISTENCE:direct assay:GO:0004073 aspartate-semialdehyde dehydrogenase activity [PMID:4145650]
EXISTENCE:mutant phenotype:GO:0009086 methionine biosynthetic process [PMID:4380684]
EXISTENCE:mutant phenotype:GO:0009088 threonine biosynthetic process [PMID:4380684]
EXISTENCE:mutant phenotype:GO:0009090 homoserine biosynthetic process [PMID:4380684]
Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:12631707]
EXISTENCE:direct assay:GO:0070390 transcription export complex 2 [PMID:15311284]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:12631707]
EXISTENCE:genetic interaction:GO:0030029 actin filament-based process [PMID:2656401]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:8799844]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:18003937]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:12411502|PMID:12631707]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:10716708]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:19806183]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:18003937]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12411502]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:12631707]
mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10409731|PMID:24124599]
EXISTENCE:mutant phenotype:GO:0043200 response to amino acid [PMID:10409731]
Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25936803|PMID:26447800|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26447800|PMID:14562095]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:25936803]
EXISTENCE:direct assay:GO:0140318 protein transporter activity [PMID:26447800]
EXISTENCE:genetic interaction:GO:0051083 'de novo' cotranslational protein folding [PMID:19325107]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:25936803|PMID:26447800]
Specific assembly chaperone for ribosomal protein Rpl4a/Rpl4b; associates co-translationally with a evolutionarily conserved internal loop of nascent Rpl4a/b, and is released only after an the eukaryotic-specific extension of nascent Rpl4a/b mediates nuclear import, incorporation into the pre-ribosome and complex disassembly; role in biogenesis of the 60S ribosomal subunit; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14573466]
EXISTENCE:genetic interaction:GO:0043409 negative regulation of MAPK cascade [PMID:14685261]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:14685261]
EXISTENCE:mutant phenotype:GO:0043409 negative regulation of MAPK cascade [PMID:22570491]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:14685261]
Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p to the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm; contains an SH3 domain and a Ptc1p binding domain (PBM)
U2-type spliceosomal complex subunit CWC15
EXISTENCE:direct assay:GO:0005684 U2-type spliceosomal complex [PMID:11884590]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:11884590]
Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:10427089]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15466482]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10427089|PMID:11038189]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10427089|PMID:11038189|PMID:26928762]
EXISTENCE:direct assay:GO:0031340 positive regulation of vesicle fusion [PMID:15466482]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:10427089]
EXISTENCE:physical interaction:GO:0006887 exocytosis [PMID:10427089]
EXISTENCE:physical interaction:GO:0006904 vesicle docking involved in exocytosis [PMID:10427089]
Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:17382321]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:12403464]
Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:8978675]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11595741]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:8978675]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11595741|PMID:17717527]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11595741|PMID:26928762]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:15583030]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0001927 exocyst assembly [PMID:15583030]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:6996832]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:7026045]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:15583030]
Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10817999]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:15448131|PMID:12138208]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:8662725]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:12840001]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:11940675]
Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9242486]
EXISTENCE:mutant phenotype:GO:0030474 spindle pole body duplication [PMID:9060463]
EXISTENCE:mutant phenotype:GO:0043410 positive regulation of MAPK cascade [PMID:17220467]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:17242065]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:7753858]
EXISTENCE:physical interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:17220467]
Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17616518|PMID:20385783|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20385783|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:17616518|PMID:19158363]
EXISTENCE:mutant phenotype:GO:0000432 positive regulation of transcription from RNA polymerase II promoter by glucose [PMID:17616518]
EXISTENCE:mutant phenotype:GO:0000976 transcription cis-regulatory region binding [PMID:17616518]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:17616518]
Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
hypothetical protein; identified by fungal homology and RT-PCR
Arf family guanine nucleotide exchange factor SEC7
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10430582|PMID:9539714]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:12006663]
EXISTENCE:direct assay:GO:0005798 Golgi-associated vesicle [PMID:1729652]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:1986005]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:1986005]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:20444982]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9473503]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:3042778]
Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
Retrotransposon TYA Gag gene; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag; YDR170W-A is part of a mutant retrotransposon; distribution in the cytoplasm becomes irregular rather than punctate upon DNA replication stress
ARS425; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:9199338]
EXISTENCE:mutant phenotype:GO:0030307 positive regulation of cell growth [PMID:31649258]
EXISTENCE:mutant phenotype:GO:0140311 protein sequestering activity [PMID:31649258]
Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
translation termination factor GTPase eRF3
EXISTENCE:direct assay:GO:0003747 translation release factor activity [PMID:20947765]
EXISTENCE:direct assay:GO:0006415 translational termination [PMID:20947765]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23451152]
EXISTENCE:direct assay:GO:0018444 translation release factor complex [PMID:7556078]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:8469113]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:12923185]
Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
EXISTENCE:direct assay:GO:0000823 inositol-1,4,5-trisphosphate 6-kinase activity [PMID:10574768|PMID:10720331]
EXISTENCE:direct assay:GO:0000824 inositol tetrakisphosphate 3-kinase activity [PMID:10574768|PMID:10720331]
EXISTENCE:direct assay:GO:0000825 inositol tetrakisphosphate 6-kinase activity [PMID:11311242]
EXISTENCE:direct assay:GO:0000827 inositol-1,3,4,5,6-pentakisphosphate kinase activity [PMID:11311242]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10720331|PMID:3311884]
EXISTENCE:direct assay:GO:0032958 inositol phosphate biosynthetic process [PMID:10574768|PMID:11311242]
EXISTENCE:direct assay:GO:0035004 phosphatidylinositol 3-kinase activity [PMID:16123124]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:16123124]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12828642]
EXISTENCE:mutant phenotype:GO:0000821 regulation of arginine metabolic process [PMID:11119723|PMID:2274024|PMID:8043104]
EXISTENCE:mutant phenotype:GO:0008440 inositol-1,4,5-trisphosphate 3-kinase activity [PMID:10683435]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:10632874]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12828642]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:10632874]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:10632874]
Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates genes responsive to arginine, phosphate, and nitrogen; needed by most [PSI+] variants for prion propagation
EXISTENCE:direct assay:GO:0000400 four-way junction DNA binding [PMID:15507436|PMID:16533046]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16612005|PMID:17646381]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:15507436|PMID:16533046]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12374750]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8969238]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:25063301]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:15507436|PMID:16533046|PMID:25063301]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:22553361]
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:18451108]
EXISTENCE:direct assay:GO:0051276 chromosome organization [PMID:25063301]
EXISTENCE:genetic interaction:GO:0006356 regulation of transcription by RNA polymerase I [PMID:12374750]
EXISTENCE:genetic interaction:GO:0060962 regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:18187511]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:18187511]
EXISTENCE:mutant phenotype:GO:0006265 DNA topological change [PMID:31969709]
EXISTENCE:mutant phenotype:GO:0006356 regulation of transcription by RNA polymerase I [PMID:16612005]
EXISTENCE:mutant phenotype:GO:0030261 chromosome condensation [PMID:27030801]
EXISTENCE:mutant phenotype:GO:0044804 autophagy of nucleus [PMID:31553911]
EXISTENCE:mutant phenotype:GO:0060962 regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:17875934|PMID:18187511]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:31980450|PMID:31553911]
Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia
mitochondrial 37S ribosomal protein RSM24
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:11773077]
EXISTENCE:direct assay:GO:0140671 ADA complex [PMID:7862114|PMID:10490601]
Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:2265617]
EXISTENCE:genetic interaction:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:2265617]
EXISTENCE:genetic interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:10878801]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:11134048|PMID:2265617]
EXISTENCE:mutant phenotype:GO:0016050 vesicle organization [PMID:11134048]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:10878801]
Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)
succinate dehydrogenase membrane anchor subunit SDH4
EXISTENCE:genetic interaction:GO:0008177 succinate dehydrogenase (ubiquinone) activity [PMID:18028869]
EXISTENCE:mutant phenotype:GO:0045333 cellular respiration [PMID:16232921]
Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma
EXISTENCE:mutant phenotype:GO:0000338 protein deneddylation [PMID:12186635|PMID:12446563]
Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:26283797]
Protein with a potential role in tethering ER and vacuoles; localizes to nucleus-vacuole junctions in an Mdm1p-dependent manner; contains a lipid-binding PXA domain
cohesin-loading factor complex subunit SCC2
EXISTENCE:curator inference:GO:0030543 2-micrometer plasmid partitioning [PMID:17670945]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:9990856]
EXISTENCE:direct assay:GO:0005729 2-micrometer circle DNA [PMID:17670945]
EXISTENCE:direct assay:GO:0043515 kinetochore binding [PMID:28938124]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:11448778|PMID:15610741|PMID:15610742]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:18708580|PMID:19948494]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:10882066]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:19948494]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:18708580]
EXISTENCE:mutant phenotype:GO:0071168 protein localization to chromatin [PMID:16793545|PMID:18708580]
EXISTENCE:mutant phenotype:GO:0071169 establishment of protein localization to chromatin [PMID:10882066]
EXISTENCE:physical interaction:GO:0032116 SMC loading complex [PMID:10882066]
Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2; conserved role in NHEJ
EXISTENCE:curator inference:GO:0000781 chromosome, telomeric region [PMID:11731479]
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:11731479]
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:12626510]
EXISTENCE:direct assay:GO:0016407 acetyltransferase activity [PMID:11731479]
EXISTENCE:direct assay:GO:0031509 subtelomeric heterochromatin formation [PMID:11731479]
EXISTENCE:direct assay:GO:0033255 SAS acetyltransferase complex [PMID:12626510]
Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:18332110]
EXISTENCE:genetic interaction:GO:0006506 GPI anchor biosynthetic process [PMID:25165136]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:7969142]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:33112703]
Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls
EXISTENCE:direct assay:GO:0031683 G-protein beta/gamma-subunit complex binding [PMID:10749875]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:16415341]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10749875]
Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10102375|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:11065362]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:10102375]
Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19506038]
EXISTENCE:genetic interaction:GO:0070584 mitochondrion morphogenesis [PMID:19506038]
EXISTENCE:mutant phenotype:GO:0070584 mitochondrion morphogenesis [PMID:19506038]
Mitochondrial hypothetical protein; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication
Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; co-purifies with ribosomes; GFP-fusion protein localizes to the cytoplasm
chaperonin-containing T-complex subunit CCT6
Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:15372079]
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:15958490]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10747087]
EXISTENCE:direct assay:GO:0019905 syntaxin binding [PMID:12186954|PMID:15372079]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:10747087]
EXISTENCE:genetic interaction:GO:0005783 endoplasmic reticulum [PMID:15958490]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9545229]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:15958490]
EXISTENCE:mutant phenotype:GO:0019905 syntaxin binding [PMID:12186954]
EXISTENCE:mutant phenotype:GO:0035543 positive regulation of SNARE complex assembly [PMID:12186954]
EXISTENCE:mutant phenotype:GO:0048280 vesicle fusion with Golgi apparatus [PMID:8636207]
Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
RuvB family ATP-dependent DNA helicase pontin
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:16299513]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10684247]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:10952318]
EXISTENCE:direct assay:GO:0043139 5'-3' DNA helicase activity [PMID:18234224]
EXISTENCE:direct assay:GO:0070209 ASTRA complex [PMID:19040720]
EXISTENCE:direct assay:GO:0097255 R2TP complex [PMID:15766533]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:18268103]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11278922]
EXISTENCE:physical interaction:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:physical interaction:GO:0006338 chromatin remodeling [PMID:14690608]
EXISTENCE:physical interaction:GO:0031011 Ino80 complex [PMID:10952318|PMID:24034245]
EXISTENCE:physical interaction:GO:0097255 R2TP complex [PMID:18268103]
ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26158509|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26158509|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:26158509]
EXISTENCE:direct assay:GO:0009299 mRNA transcription [PMID:31167142]
EXISTENCE:direct assay:GO:0017136 NAD-dependent histone deacetylase activity [PMID:21401809]
EXISTENCE:direct assay:GO:0098781 ncRNA transcription [PMID:31167142]
EXISTENCE:genetic interaction:GO:0031509 subtelomeric heterochromatin formation [PMID:7498786]
EXISTENCE:mutant phenotype:GO:0046459 short-chain fatty acid metabolic process [PMID:12618394]
EXISTENCE:mutant phenotype:GO:1990414 replication-born double-strand break repair via sister chromatid exchange [PMID:23357952]
NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10610322|PMID:10684247|PMID:7634338]
EXISTENCE:direct assay:GO:0044614 nuclear pore cytoplasmic filaments [PMID:10610322|PMID:10684247]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:10952996]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20098417|PMID:20932479]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809|PMID:9353254|PMID:9353255]
EXISTENCE:mutant phenotype:GO:0071472 cellular response to salt stress [PMID:22851651]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17803941|PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p; human homolog NUP42 can complement yeast mutant
EXISTENCE:direct assay:GO:0000373 Group II intron splicing [PMID:17081564]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:17081564]
EXISTENCE:direct assay:GO:0003724 RNA helicase activity [PMID:17081564]
EXISTENCE:direct assay:GO:0006392 transcription elongation by mitochondrial RNA polymerase [PMID:24732805]
EXISTENCE:direct assay:GO:0033592 RNA strand annealing activity [PMID:17081564]
EXISTENCE:mutant phenotype:GO:0000372 Group I intron splicing [PMID:15618406]
EXISTENCE:mutant phenotype:GO:0000373 Group II intron splicing [PMID:15618406]
EXISTENCE:mutant phenotype:GO:0000963 mitochondrial RNA processing [PMID:15618406]
EXISTENCE:mutant phenotype:GO:0005759 mitochondrial matrix [PMID:2535893]
EXISTENCE:mutant phenotype:GO:0006392 transcription elongation by mitochondrial RNA polymerase [PMID:24732805]
EXISTENCE:mutant phenotype:GO:0034337 RNA folding [PMID:21045551]
Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12819204]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:7862160]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:12819204]
EXISTENCE:mutant phenotype:GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled [PMID:12819204]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:7862160]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:12773397]
EXISTENCE:mutant phenotype:GO:1900364 negative regulation of mRNA polyadenylation [PMID:15121841]
EXISTENCE:mutant phenotype:GO:1901901 regulation of protein localization to cell division site involved in cytokinesis [PMID:26771880]
EXISTENCE:mutant phenotype:GO:1903501 positive regulation of mitotic actomyosin contractile ring assembly [PMID:26771880]
RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14690591]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
EXISTENCE:direct assay:GO:1990143 CoA-synthesizing protein complex [PMID:23789928]
EXISTENCE:genetic interaction:GO:0015937 coenzyme A biosynthetic process [PMID:19266201]
Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; involved in histone acylation; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:1745228]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:3329045]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:3329045]
EXISTENCE:physical interaction:GO:0005761 mitochondrial ribosome [PMID:15127226]
Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:17005568]
EXISTENCE:direct assay:GO:0018026 peptidyl-lysine monomethylation [PMID:17005568]
EXISTENCE:mutant phenotype:GO:0018023 peptidyl-lysine trimethylation [PMID:17005568]
Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23625923|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:12514182]
EXISTENCE:direct assay:GO:0005793 endoplasmic reticulum-Golgi intermediate compartment [PMID:22782902]
EXISTENCE:genetic interaction:GO:0000321 re-entry into mitotic cell cycle after pheromone arrest [PMID:12588993]
EXISTENCE:genetic interaction:GO:0031929 TOR signaling [PMID:23625923]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12134085]
Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0042729 DASH complex [PMID:15640796|PMID:15664196]
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
EXISTENCE:genetic interaction:GO:0007035 vacuolar acidification [PMID:11283612|PMID:11844802]
EXISTENCE:genetic interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:11283612|PMID:11844802]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:11283612|PMID:11844802]
EXISTENCE:mutant phenotype:GO:0043254 regulation of protein-containing complex assembly [PMID:11844802]
EXISTENCE:physical interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:11283612]
EXISTENCE:physical interaction:GO:0043291 RAVE complex [PMID:11283612]
Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11469793]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:11469793|PMID:9266513]
Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15277543|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005385 zinc ion transmembrane transporter activity [PMID:11058603]
EXISTENCE:mutant phenotype:GO:0006829 zinc ion transport [PMID:15277543]
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:11058603]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15277543]
Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
EXISTENCE:direct assay:GO:0000932 P-body [PMID:17984081]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:16467471|PMID:17984081]
EXISTENCE:mutant phenotype:GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [PMID:17984081]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:23390378]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:16467471]
Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication
DNA-binding transcriptional regulator UME6
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7926768|PMID:8528081]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:11081629|PMID:7926768]
EXISTENCE:direct assay:GO:0033698 Rpd3L complex [PMID:16314178|PMID:19040720|PMID:16286007]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
EXISTENCE:direct assay:GO:0140297 DNA-binding transcription factor binding [PMID:11081629]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:7926768|PMID:8618927|PMID:24881874|PMID:8614637]
EXISTENCE:mutant phenotype:GO:0001081 nitrogen catabolite repression of transcription from RNA polymerase II promoter [PMID:1579492]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:7926768]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23478442]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:20876298]
EXISTENCE:mutant phenotype:GO:0009847 spore germination [PMID:21059190]
EXISTENCE:mutant phenotype:GO:0010673 positive regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:21123654|PMID:8618927]
EXISTENCE:mutant phenotype:GO:0010674 negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle [PMID:21123654|PMID:8618927]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:18156287]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8614637]
EXISTENCE:physical interaction:GO:0140297 DNA-binding transcription factor binding [PMID:9150136]
Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis
1-phosphatidylinositol-4-phosphate 5-kinase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9624177]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:9624177|PMID:22562153]
EXISTENCE:direct assay:GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity [PMID:9624178|PMID:9624177]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:22562153]
EXISTENCE:direct assay:GO:0046854 phosphatidylinositol phosphate biosynthetic process [PMID:9624177|PMID:9624178]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:22562153]
EXISTENCE:genetic interaction:GO:0031321 ascospore-type prospore assembly [PMID:19502581]
Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
hypothetical protein; conserved among S. cerevisiae strains; YDR209C is not an essential gene; partially overlaps uncharacterized gene YDR210W
Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YDRWTy2-2; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
YDRCTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
translation initiation factor eIF2B catalytic subunit epsilon
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:9472020]
EXISTENCE:direct assay:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705|PMID:8506384]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:8506384]
EXISTENCE:direct assay:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8506384|PMID:9472020]
EXISTENCE:genetic interaction:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:genetic interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705]
EXISTENCE:genetic interaction:GO:0006446 regulation of translational initiation [PMID:8336705]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:10805739|PMID:12356745]
EXISTENCE:mutant phenotype:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8441423]
EXISTENCE:physical interaction:GO:0003743 translation initiation factor activity [PMID:8336705]
EXISTENCE:physical interaction:GO:0005851 eukaryotic translation initiation factor 2B complex [PMID:8336705|PMID:8506384]
EXISTENCE:physical interaction:GO:0006446 regulation of translational initiation [PMID:8336705]
EXISTENCE:physical interaction:GO:0032045 guanyl-nucleotide exchange factor complex [PMID:8336705|PMID:9472020]
Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; mutations in human ortholog cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); forms cytoplasmic foci upon DNA replication stress
chaperonin-containing T-complex alpha subunit TCP1
Alpha subunit of chaperonin-containing T-complex; complex mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11533229]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11533229]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18675371]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:18675371]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26448198]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:16055745]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11533229|PMID:12077145|PMID:16055745|PMID:11238402|P MID:26448198]
EXISTENCE:mutant phenotype:GO:0071456 cellular response to hypoxia [PMID:19807692]
Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:12504007]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:36260391]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:36260391]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:12504007]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:12504007]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:36260391]
EXISTENCE:physical interaction:GO:0005737 cytoplasm [PMID:12504007]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:12504007]
Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; involved in the nuclear import of Hsp82p after DNA damage; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
hypothetical protein; conserved across S. cerevisiae strains; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:10536147|PMID:12748191|PMID:1448103|PMID:1899286|PMI D:1996109|PMID:9300483]
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:10536147]
EXISTENCE:direct assay:GO:0001093 TFIIB-class transcription factor binding [PMID:8943299]
EXISTENCE:direct assay:GO:0001094 TFIID-class transcription factor complex binding [PMID:9742103]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10608811|PMID:27663510]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:10713069]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:1355328]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:7500953|PMID:10608811|PMID:1355328|PMID:7760841|PMID :8358828|PMID:1899286]
EXISTENCE:mutant phenotype:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:12748191|PMID:1355328|PMID:11431484]
EXISTENCE:physical interaction:GO:0003713 transcription coactivator activity [PMID:8943299]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:8943299]
Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
ARS427; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:17721446]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:17726056]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:17721446]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:16219777|PMID:2664461]
EXISTENCE:genetic interaction:GO:0051726 regulation of cell cycle [PMID:2664461]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:9564050]
EXISTENCE:mutant phenotype:GO:0000785 chromatin [PMID:17721446]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:2664461]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:11267834]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:26490958]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:24302356]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:17721446|PMID:16166626]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:16166626|PMID:17721446]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8670896]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:2664461]
EXISTENCE:mutant phenotype:GO:0110027 negative regulation of DNA strand resection involved in replication fork processing [PMID:25637499]
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; protects double-strand breaks from premature resection; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:8885406]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18826657]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:18826657]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18826657|PMID:24390141]
EXISTENCE:direct assay:GO:0072687 meiotic spindle [PMID:18826657]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:18826657]
Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:26839174]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:16790494]
EXISTENCE:mutant phenotype:GO:0048312 intracellular distribution of mitochondria [PMID:26839174|PMID:35281729]
Mitochondria-associated F-box protein; tether that anchors higher-functioning mitochondria at the mother cell distal tip throughout the cell cycle and at the bud tip prior to cytokinesis; role in maintenance of normal mitochondrial morphology and quality control; interacts with Skp1p through the F-box motif; asymmetrically localized to the mother cell through the cell cycle with some bud tip localization just before cytokinesis; function regulated by the bud site selection machinery
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:16373354]
EXISTENCE:mutant phenotype:GO:0000271 polysaccharide biosynthetic process [PMID:16373354]
EXISTENCE:mutant phenotype:GO:0004558 alpha-1,4-glucosidase activity [PMID:16373354|PMID:19542522]
EXISTENCE:mutant phenotype:GO:0006491 N-glycan processing [PMID:16373354]
EXISTENCE:physical interaction:GO:0017177 glucosidase II complex [PMID:16373354]
Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:15620355]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:15620355]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15620355]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15620355]
EXISTENCE:mutant phenotype:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15620355]
EXISTENCE:physical interaction:GO:0010688 negative regulation of ribosomal protein gene transcription by RNA polymerase II [PMID:15620355]
Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication
ARS428; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000786 nucleosome [PMID:9256430]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:8017102]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9256430|PMID:8017102]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:11973294]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:17526727]
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15781691]
Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:20659900]
EXISTENCE:direct assay:GO:0004017 adenylate kinase activity [PMID:15753074|PMID:18433446]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:2850178]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:2850178]
EXISTENCE:direct assay:GO:0006172 ADP biosynthetic process [PMID:2848829]
EXISTENCE:mutant phenotype:GO:0004017 adenylate kinase activity [PMID:18433446|PMID:2850178]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:20980819]
EXISTENCE:mutant phenotype:GO:0009117 nucleotide metabolic process [PMID:2850178]
EXISTENCE:mutant phenotype:GO:0036388 pre-replicative complex assembly [PMID:20659900]
Adenylate kinase, required for purine metabolism; controls ATP homeostasis; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:19217406|PMID:22654676]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:22654676]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:22654676]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:22654676|PMID:3297920|PMID:26587833]
EXISTENCE:mutant phenotype:GO:0031453 positive regulation of heterochromatin formation [PMID:26587833]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:22654676|PMID:9501103|PMID:1913809|PMID:26587833]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:26399229|PMID:27122604]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:12080091]
EXISTENCE:mutant phenotype:GO:0097695 establishment of protein-containing complex localization to telomere [PMID:29290466|PMID:26218225]
SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some SIR4 alleles prolong lifespan; required for Ku-mediated telomerase recruitment, telomere lengthening, and telomere hypercluster formation in quiescent yeast cells
ARS429; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:12727883]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:15998810]
EXISTENCE:direct assay:GO:0006369 termination of RNA polymerase II transcription [PMID:12727883|PMID:15998810]
EXISTENCE:direct assay:GO:0006378 mRNA polyadenylation [PMID:11344258]
EXISTENCE:direct assay:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:11344258]
EXISTENCE:mutant phenotype:GO:0000993 RNA polymerase II complex binding [PMID:15998810]
EXISTENCE:mutant phenotype:GO:0003729 mRNA binding [PMID:15998810]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:15998810]
EXISTENCE:mutant phenotype:GO:0030847 termination of RNA polymerase II transcription, exosome-dependent [PMID:12819204]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:15998810]
EXISTENCE:physical interaction:GO:0005849 mRNA cleavage factor complex [PMID:11344258]
mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12553664|PMID:23836928|PMID:26928762]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:12553664]
EXISTENCE:genetic interaction:GO:0042144 vacuole fusion, non-autophagic [PMID:12553664]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:12553664]
EXISTENCE:physical interaction:GO:0042144 vacuole fusion, non-autophagic [PMID:12553664]
Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase; contains a putative inverse BAR (I-BAR) domain that can mold lipid bilayer membranes into protrusions
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10671482]
EXISTENCE:direct assay:GO:0043069 negative regulation of programmed cell death [PMID:31752220]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10671482]
EXISTENCE:direct assay:GO:1902884 positive regulation of response to oxidative stress [PMID:31752220]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:10671482]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:10671482]
EXISTENCE:mutant phenotype:GO:0043069 negative regulation of programmed cell death [PMID:31752220]
EXISTENCE:mutant phenotype:GO:1902884 positive regulation of response to oxidative stress [PMID:31752220]
Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
EXISTENCE:direct assay:GO:0003870 5-aminolevulinate synthase activity [PMID:6381051]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:3023841]
EXISTENCE:mutant phenotype:GO:0003870 5-aminolevulinate synthase activity [PMID:323256|PMID:24173275|PMID:7035824]
EXISTENCE:mutant phenotype:GO:0006783 heme biosynthetic process [PMID:323256|PMID:7035824]
EXISTENCE:mutant phenotype:GO:1902117 positive regulation of organelle assembly [PMID:32075909]
5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p; has a pyridoxal phosphate cofactor whose insertion is mediated by Mcx1p
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16002643|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16624861]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:16002643]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:16624861]
EXISTENCE:genetic interaction:GO:0051292 nuclear pore complex assembly [PMID:19273614]
EXISTENCE:genetic interaction:GO:0071786 endoplasmic reticulum tubular network organization [PMID:18309084|PMID:18442980|PMID:19665976|PMID:16469703]
EXISTENCE:physical interaction:GO:0071786 endoplasmic reticulum tubular network organization [PMID:19665976]
Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; increases tubular ER when overexpressed; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; member of the RTNLA subfamily
EXISTENCE:mutant phenotype:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:2507177]
Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9418882]
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9418882]
U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:10887197]
EXISTENCE:direct assay:GO:0008531 riboflavin kinase activity [PMID:10887197]
EXISTENCE:direct assay:GO:0009398 FMN biosynthetic process [PMID:10887197]
EXISTENCE:direct assay:GO:0043231 intracellular membrane-bounded organelle [PMID:10887197]
EXISTENCE:mutant phenotype:GO:0008531 riboflavin kinase activity [PMID:10887197]
EXISTENCE:mutant phenotype:GO:0009398 FMN biosynthetic process [PMID:10887197]
Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane; human RFK functionally complements the lethality of the null mutation
mitochondrial 54S ribosomal protein YmL7/YmL5
Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:7929113]
Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
hypothetical protein; may interact with ribosomes, based on co-purification experiments
EXISTENCE:physical interaction:GO:0000243 commitment complex [PMID:10072386]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:10072386]
EXISTENCE:physical interaction:GO:0003729 mRNA binding [PMID:10072386]
Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
Tyrosine tRNA (tRNA-Tyr), predicted by tRNAscan-SE analysis; can mutate to suppress ochre nonsense mutations
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:23462954]
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720284]
EXISTENCE:mutant phenotype:GO:0000384 first spliceosomal transesterification activity [PMID:7520570]
EXISTENCE:mutant phenotype:GO:0000395 mRNA 5'-splice site recognition [PMID:23462954]
Pre-mRNA splicing factor; RNA binding protein involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family
EXISTENCE:direct assay:GO:0005052 peroxisome matrix targeting signal-1 binding [PMID:7980572]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:15632140]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:16849337]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:15283676]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15283676|PMID:8858166]
EXISTENCE:direct assay:GO:1990429 peroxisomal importomer complex [PMID:22375831]
EXISTENCE:genetic interaction:GO:0016558 protein import into peroxisome matrix [PMID:27678487]
EXISTENCE:mutant phenotype:GO:0005052 peroxisome matrix targeting signal-1 binding [PMID:11154700]
EXISTENCE:mutant phenotype:GO:0016560 protein import into peroxisome matrix, docking [PMID:16849337]
EXISTENCE:physical interaction:GO:0005778 peroxisomal membrane [PMID:8858166]
EXISTENCE:physical interaction:GO:0016560 protein import into peroxisome matrix, docking [PMID:12595255|PMID:16849337]
EXISTENCE:physical interaction:GO:0030674 protein-macromolecule adaptor activity [PMID:15798189]
Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
EXISTENCE:direct assay:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:10037752]
EXISTENCE:direct assay:GO:0000136 mannan polymerase complex [PMID:10037752]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:10037752]
EXISTENCE:mutant phenotype:GO:0000917 division septum assembly [PMID:16034811]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:8991513|PMID:23210626]
Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17110339]
EXISTENCE:direct assay:GO:1990070 TRAPPI protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9564032]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17287728]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:18439903]
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4
putative serine/threonine protein kinase VHS1
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:29249654]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:29249654]
Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0046316 gluconokinase activity [PMID:26279678]
Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:32491201]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:32491201]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8106383]
Essential hypothetical protein; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005854 nascent polypeptide-associated complex [PMID:10219998]
EXISTENCE:genetic interaction:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:10518932]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:10219998]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:18214544]
Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
EXISTENCE:direct assay:GO:0000987 cis-regulatory region sequence-specific DNA binding [PMID:9199298]
EXISTENCE:direct assay:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9199298]
EXISTENCE:direct assay:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:direct assay:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9799240]
EXISTENCE:genetic interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
EXISTENCE:genetic interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9199298]
EXISTENCE:genetic interaction:GO:0007346 regulation of mitotic cell cycle [PMID:18308733]
EXISTENCE:genetic interaction:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:22696683]
EXISTENCE:mutant phenotype:GO:0007346 regulation of mitotic cell cycle [PMID:18308733]
EXISTENCE:mutant phenotype:GO:0031335 regulation of sulfur amino acid metabolic process [PMID:9199298]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22696683]
EXISTENCE:physical interaction:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9799240]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9799240]
Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000940 outer kinetochore [PMID:12589047]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:12589047|PMID:12642611]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:8243998|PMID:9339342]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:22426531]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0034089 establishment of meiotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0034090 maintenance of meiotic sister chromatid cohesion [PMID:14752166]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:12642611]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:16357219|PMID:26653857]
Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27663510]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12686616]
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:12686616|PMID:16872538]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:18508925]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:18508925]
Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
EXISTENCE:direct assay:GO:0004096 catalase activity [PMID:9931255]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:14998369]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:1986244]
EXISTENCE:direct assay:GO:0042744 hydrogen peroxide catabolic process [PMID:9931255]
EXISTENCE:genetic interaction:GO:0004096 catalase activity [PMID:3897793]
EXISTENCE:genetic interaction:GO:0042744 hydrogen peroxide catabolic process [PMID:3897793]
EXISTENCE:mutant phenotype:GO:0000302 response to reactive oxygen species [PMID:15893481]
EXISTENCE:mutant phenotype:GO:0004096 catalase activity [PMID:3897793]
EXISTENCE:mutant phenotype:GO:0042744 hydrogen peroxide catabolic process [PMID:3897793]
Catalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation
EXISTENCE:genetic interaction:GO:0016279 protein-lysine N-methyltransferase activity [PMID:18957409]
EXISTENCE:genetic interaction:GO:0018026 peptidyl-lysine monomethylation [PMID:18957409]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:18957409]
EXISTENCE:mutant phenotype:GO:0018026 peptidyl-lysine monomethylation [PMID:18957409|PMID:24517342]
Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:8413229]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:11734006]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:11734006]
EXISTENCE:direct assay:GO:0051787 misfolded protein binding [PMID:7628444]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:7500331]
EXISTENCE:genetic interaction:GO:0034605 cellular response to heat [PMID:8830768]
EXISTENCE:genetic interaction:GO:0050821 protein stabilization [PMID:7628444]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:11734006]
EXISTENCE:mutant phenotype:GO:0034605 cellular response to heat [PMID:7628444|PMID:8830768]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:11734006]
EXISTENCE:mutant phenotype:GO:0043335 protein unfolding [PMID:16460754]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:16460754]
Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded proteins as well as resolubilize protein aggregates; involved in response to ethanol stress
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:18287073]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11179441]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18287073]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18287073]
EXISTENCE:physical interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:21552514]
EXISTENCE:physical interaction:GO:0001010 RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity [PMID:21552514]
Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10022899]
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:12609981|PMID:15060174|PMID:12574115]
EXISTENCE:mutant phenotype:GO:0030071 regulation of mitotic metaphase/anaphase transition [PMID:12609981]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:10022899]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:15060174]
EXISTENCE:mutant phenotype:GO:1904824 anaphase-promoting complex assembly [PMID:15060174]
Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
EXISTENCE:curator inference:GO:0031505 fungal-type cell wall organization [PMID:7875558]
EXISTENCE:direct assay:GO:0004338 glucan exo-1,3-beta-glucosidase activity [PMID:7875558]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:7875558]
Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YDRWTy2-3; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
YDRCTy1-3; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; mutated in S288C
Retrotransposon TYA Gag and TYB Pol genes; Gag processing produces capsid proteins, Pol is cleaved to produce protease, reverse transcriptase and integrase activities; in YDRCTy1-3 TYB is mutant and probably non-functional
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; Gag processing produces capsid proteins; in YDRCTy1-3 TYB is mutant and probably non-functional
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10628970]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:9079876]
EXISTENCE:mutant phenotype:GO:0035312 5'-3' exodeoxyribonuclease activity [PMID:23598996]
Mitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:16647879]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:12370247]
EXISTENCE:direct assay:GO:0018345 protein palmitoylation [PMID:12370247|PMID:16227572]
EXISTENCE:genetic interaction:GO:0090029 negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8524293]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:16227572]
EXISTENCE:mutant phenotype:GO:0018345 protein palmitoylation [PMID:16227572|PMID:16751107]
EXISTENCE:mutant phenotype:GO:0030100 regulation of endocytosis [PMID:10866691]
EXISTENCE:mutant phenotype:GO:0090029 negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8649369]
EXISTENCE:physical interaction:GO:0031683 G-protein beta/gamma-subunit complex binding [PMID:8649369]
Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:24034674]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:27678487|PMID:12876220]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:18644345]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:18644345|PMID:19687296|PMID:22471590]
EXISTENCE:mutant phenotype:GO:0016558 protein import into peroxisome matrix [PMID:15536088]
Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders
EXISTENCE:direct assay:GO:0016574 histone ubiquitination [PMID:22570702]
EXISTENCE:direct assay:GO:0061630 ubiquitin protein ligase activity [PMID:22570702]
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:31532761]
EXISTENCE:mutant phenotype:GO:0036205 histone catabolic process [PMID:22570702]
EXISTENCE:mutant phenotype:GO:0061157 mRNA destabilization [PMID:31532761]
RING finger ubiquitin ligase (E3); subunit of ribosome-associated quality control trigger complex (RQT); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; required to trigger ribosome-associated quality control (RQC) and canonical no-go decay; ubiquitinates Rps20 and Rps3; binds to ribosomal RNA, mRNA and tRNA
iron-sulfur cluster assembly protein CIA1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16314508|PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16314508]
EXISTENCE:direct assay:GO:0097361 CIA complex [PMID:22678362]
EXISTENCE:genetic interaction:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:17937914]
EXISTENCE:mutant phenotype:GO:0097428 protein maturation by iron-sulfur cluster transfer [PMID:16314508|PMID:17937914]
Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at late step of Fe-S cluster assembly; forms CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription and telomere maintenance; contains WD40 repeats; human homolog CIAO1 complements the yeast cia1 null mutant
EXISTENCE:mutant phenotype:GO:0004830 tryptophan-tRNA ligase activity [PMID:2999114]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:2999114]
EXISTENCE:mutant phenotype:GO:0070183 mitochondrial tryptophanyl-tRNA aminoacylation [PMID:2999114]
Mitochondrial tryptophanyl-tRNA synthetase
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:17961510]
EXISTENCE:direct assay:GO:0012510 trans-Golgi network transport vesicle membrane [PMID:9325307]
EXISTENCE:direct assay:GO:0019829 ATPase-coupled cation transmembrane transporter activity [PMID:15078884]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:7708696]
EXISTENCE:mutant phenotype:GO:0019829 ATPase-coupled cation transmembrane transporter activity [PMID:7708696]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:7708696]
EXISTENCE:mutant phenotype:GO:0060003 copper ion export [PMID:7708696]
Cu(+2)-transporting P-type ATPase; required for export of copper from cytosol into extracytosolic compartment; targeted to vacuole via AP-3 pathway; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant
EXISTENCE:direct assay:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9261170]
EXISTENCE:direct assay:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
EXISTENCE:genetic interaction:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:genetic interaction:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
EXISTENCE:mutant phenotype:GO:0004416 hydroxyacylglutathione hydrolase activity [PMID:9261170]
EXISTENCE:mutant phenotype:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:9261170]
Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070056 prospore membrane leading edge [PMID:10899120]
EXISTENCE:physical interaction:GO:0030476 ascospore wall assembly [PMID:11973299]
Meiosis-specific component of the spindle pole body; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane (PSM) during meiosis II; required for PSM growth and closure; DON1 has a paralog, CUE5, that arose from the whole genome
hypothetical protein; conserved among S. cerevisiae strains; YDR274C is not an essential gene
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:14562095]
hypothetical protein involved in multidrug resistance; inhibits oxidative damage induced by amphotericin B; overexpression increases flocculation, biofilm formation, invasive and pseudohyphal growth; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10835350]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:26303201]
EXISTENCE:direct assay:GO:0046625 sphingolipid binding [PMID:26303201]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:26303201]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:26303201]
EXISTENCE:mutant phenotype:GO:0006812 cation transport [PMID:10835350]
EXISTENCE:mutant phenotype:GO:0042391 regulation of membrane potential [PMID:10835350]
EXISTENCE:mutant phenotype:GO:0071466 cellular response to xenobiotic stimulus [PMID:23416056]
Small plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:10629208]
EXISTENCE:mutant phenotype:GO:1904659 glucose transmembrane transport [PMID:10629208]
Negative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication
ARS430; Autonomously Replicating Sequence
hypothetical protein; conserved among S. cerevisiae strains; YDR278C is not an essential gene
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis
EXISTENCE:curator inference:GO:0043137 DNA replication, removal of RNA primer [PMID:14734815]
EXISTENCE:direct assay:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
EXISTENCE:direct assay:GO:0006401 RNA catabolic process [PMID:14734815]
EXISTENCE:direct assay:GO:0032299 ribonuclease H2 complex [PMID:14734815]
EXISTENCE:genetic interaction:GO:0004523 RNA-DNA hybrid ribonuclease activity [PMID:14734815]
Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome
exosome non-catalytic core subunit RRP45
EXISTENCE:genetic interaction:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:genetic interaction:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10465791|PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034473 U1 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034475 U4 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0034476 U5 snRNA 3'-end processing [PMID:10508172]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:10465791]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
hypothetical protein; expression is regulated by phosphate levels
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:23022099]
Mitochondrial inner membrane hypothetical protein; associates with mitochondrial ribosome; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:7623840]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:28057755]
EXISTENCE:direct assay:GO:0004694 eukaryotic translation initiation factor 2alpha kinase activity [PMID:8798780]
EXISTENCE:direct assay:GO:0006446 regulation of translational initiation [PMID:7934812]
EXISTENCE:direct assay:GO:0016239 positive regulation of macroautophagy [PMID:28057755]
EXISTENCE:direct assay:GO:0022626 cytosolic ribosome [PMID:2038314]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059]
EXISTENCE:mutant phenotype:GO:0000049 tRNA binding [PMID:7623840]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:17890903]
EXISTENCE:mutant phenotype:GO:0004672 protein kinase activity [PMID:28057755]
EXISTENCE:mutant phenotype:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:6351059|PMID:7623840]
EXISTENCE:mutant phenotype:GO:1904262 negative regulation of TORC1 signaling [PMID:28057755]
Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11139591|PMID:26928762|PMID:23836928]
EXISTENCE:direct assay:GO:0000810 diacylglycerol diphosphate phosphatase activity [PMID:8567632]
EXISTENCE:direct assay:GO:0008195 phosphatidate phosphatase activity [PMID:10329682]
EXISTENCE:mutant phenotype:GO:0000810 diacylglycerol diphosphate phosphatase activity [PMID:9452443]
EXISTENCE:mutant phenotype:GO:0006644 phospholipid metabolic process [PMID:10329682]
Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:26653857]
EXISTENCE:direct assay:GO:0000795 synaptonemal complex [PMID:24098146]
EXISTENCE:direct assay:GO:0000802 transverse filament [PMID:10662769]
EXISTENCE:genetic interaction:GO:0098530 positive regulation of strand invasion [PMID:32386601]
EXISTENCE:mutant phenotype:GO:0007129 homologous chromosome pairing at meiosis [PMID:15805472|PMID:9611194]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15066280]
EXISTENCE:mutant phenotype:GO:0033235 positive regulation of protein sumoylation [PMID:23326245|PMID:27184389]
EXISTENCE:mutant phenotype:GO:0045128 negative regulation of reciprocal meiotic recombination [PMID:26653857]
EXISTENCE:mutant phenotype:GO:1990166 protein localization to site of double-strand break [PMID:23593021]
EXISTENCE:physical interaction:GO:0032184 SUMO polymer binding [PMID:16847351]
Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-allelc sites; potential Cdc28p substrate
Mitochondrial hypothetical protein; predicted to have thiol-disulfide oxidoreductase active site
inositol monophosphate 1-phosphatase INM2
EXISTENCE:direct assay:GO:0008934 inositol monophosphate 1-phosphatase activity [PMID:10096091]
EXISTENCE:direct assay:GO:0046855 inositol phosphate dephosphorylation [PMID:10096091]
EXISTENCE:genetic interaction:GO:0008934 inositol monophosphate 1-phosphatase activity [PMID:12593845]
EXISTENCE:genetic interaction:GO:0046855 inositol phosphate dephosphorylation [PMID:12593845]
EXISTENCE:mutant phenotype:GO:0008934 inositol monophosphate 1-phosphatase activity [PMID:12593845]
EXISTENCE:mutant phenotype:GO:0046855 inositol phosphate dephosphorylation [PMID:12593845]
Inositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15738391]
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:15565157]
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:20818393]
EXISTENCE:direct assay:GO:0035861 site of double-strand break [PMID:22355353]
EXISTENCE:genetic interaction:GO:0031124 mRNA 3'-end processing [PMID:15565157]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:11779788]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15565157]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:15565157]
Protein involved in transcription termination by RNA polymerase II; interacts with exonuclease Rat1p and Rai1p; has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:24440721]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:32371399]
EXISTENCE:direct assay:GO:0032075 positive regulation of nuclease activity [PMID:32371399]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:23026052]
EXISTENCE:direct assay:GO:0036297 interstrand cross-link repair [PMID:32371399]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:30068549|PMID:24440721]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:23026052|PMID:24440721|PMID:28385527]
EXISTENCE:genetic interaction:GO:0006289 nucleotide-excision repair [PMID:24682993]
EXISTENCE:genetic interaction:GO:0032204 regulation of telomere maintenance [PMID:24440721]
EXISTENCE:mutant phenotype:GO:0032075 positive regulation of nuclease activity [PMID:32371399]
EXISTENCE:mutant phenotype:GO:0032202 telomere assembly [PMID:24440721]
EXISTENCE:physical interaction:GO:0006289 nucleotide-excision repair [PMID:24682993]
3'-5' DNA helicase of the conserved RecQ family; involved in Pso2p-mediated interstrand crosslink repair; acts with Rad4p in nucleotide-excision repair; binds telomeres, modulates telomerase activity with Pif1p and inhibits telomere addition to dsDNA breaks; role in telomerase-independent telomere maintenance; lacks ssDNA annealing and strand exchange activities; human RecQL4, a structural and functional homolog, is involved in Rothmund-Thomson, Baller-Gerold and RAPADILINO syndromes
Signal recognition particle receptor subunit alpha
EXISTENCE:curator inference:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:7844142]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9679135]
EXISTENCE:mutant phenotype:GO:0005047 signal recognition particle binding [PMID:1327299]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:1327299]
EXISTENCE:physical interaction:GO:0005785 signal recognition particle receptor complex [PMID:9679135]
Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
mRNA-binding translational repressor SSD1
EXISTENCE:direct assay:GO:0000932 P-body [PMID:19962308|PMID:21339329]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:19962308|PMID:23222640|PMID:31909711]
EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR binding [PMID:24465507]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21762218]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19962308|PMID:9195905]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:21339329]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:21339329]
EXISTENCE:direct assay:GO:0008298 intracellular mRNA localization [PMID:31909711]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:21339329]
EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR binding [PMID:20977549]
EXISTENCE:genetic interaction:GO:0000900 mRNA regulatory element binding translation repressor activity [PMID:19962308]
EXISTENCE:genetic interaction:GO:0017148 negative regulation of translation [PMID:19962308]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:19962308]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:21339329]
EXISTENCE:mutant phenotype:GO:1903450 regulation of G1 to G0 transition [PMID:31141453]
Translational repressor; involved in polar growth, wall integrity, autodiploidization; regulated by Cbk1p phosphorylation to affect bud-specific translational control and localization of specific mRNAs; binds 5'UTRs of mRNAs encoding cell wall proteins; recognizes CNYTCNYT motif in RNA; interacts with TOR pathway components; nucleocytoplasmic shuttling appears to be critical to Ssd1p function; W303 lacks a functional SSD1 gene product; pseudonuclease descended from Dis3L2-family nucleases
EXISTENCE:direct assay:GO:0008117 sphinganine-1-phosphate aldolase activity [PMID:9334171]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:18487605]
EXISTENCE:direct assay:GO:0097038 perinuclear endoplasmic reticulum [PMID:18487605]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:9334171]
EXISTENCE:mutant phenotype:GO:0009267 cellular response to starvation [PMID:10329480]
EXISTENCE:mutant phenotype:GO:0019722 calcium-mediated signaling [PMID:11278643]
Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate; localizes to the ER and to the peroxisome
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:19573535]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:16415367]
EXISTENCE:direct assay:GO:0004407 histone deacetylase activity [PMID:19573535]
EXISTENCE:direct assay:GO:0070823 HDA1 complex [PMID:11287668]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11287668]
EXISTENCE:mutant phenotype:GO:0031047 RNA-mediated gene silencing [PMID:18022365]
EXISTENCE:physical interaction:GO:0070823 HDA1 complex [PMID:11287668]
Subunit of HDA1 histone deacetylase complex; tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; Hda2p relocalizes to cytosol in response to hypoxia; other members of the HDA1 histone deacetylase complex, which regulates aging through trehalose metabolism, are Hda1p and Hda3p
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:24675956]
EXISTENCE:direct assay:GO:0000002 mitochondrial genome maintenance [PMID:12034822]
EXISTENCE:direct assay:GO:0000150 DNA strand exchange activity [PMID:12198175]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12198175]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:19729448]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:24675956]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12034822]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12034822|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:24675956]
EXISTENCE:genetic interaction:GO:0000002 mitochondrial genome maintenance [PMID:12198175]
EXISTENCE:mutant phenotype:GO:0000150 DNA strand exchange activity [PMID:12198175]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:12198175]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:19074198]
EXISTENCE:mutant phenotype:GO:0090297 positive regulation of mitochondrial DNA replication [PMID:17116696|PMID:19074198]
EXISTENCE:physical interaction:GO:0006355 regulation of DNA-templated transcription [PMID:9736700]
Mitochondrial ribosomal protein of the large subunit; also involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8868422]
EXISTENCE:mutant phenotype:GO:0000170 sphingosine hydroxylase activity [PMID:9556590]
EXISTENCE:mutant phenotype:GO:0006665 sphingolipid metabolic process [PMID:9556590]
Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
ARS431; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000274 mitochondrial proton-transporting ATP synthase, stator stalk [PMID:18722382]
EXISTENCE:mutant phenotype:GO:0000274 mitochondrial proton-transporting ATP synthase, stator stalk [PMID:2146269]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:15254373|PMID:2146269]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:7866306]
Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:24357410]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0032040 small-subunit processome [PMID:24357410]
Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
EXISTENCE:direct assay:GO:0004349 glutamate 5-kinase activity [PMID:12513997]
EXISTENCE:direct assay:GO:0006561 proline biosynthetic process [PMID:12513997]
EXISTENCE:genetic interaction:GO:0034517 ribophagy [PMID:27442630]
EXISTENCE:mutant phenotype:GO:0004349 glutamate 5-kinase activity [PMID:2824433]
EXISTENCE:mutant phenotype:GO:0034517 ribophagy [PMID:27442630]
Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:9099738]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:10523662|PMID:12819204]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:12145212]
RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
mannose-ethanolamine phosphotransferase GPI11
EXISTENCE:curator inference:GO:0005789 endoplasmic reticulum membrane [PMID:10793139]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:10793139]
EXISTENCE:mutant phenotype:GO:0051377 mannose-ethanolamine phosphotransferase activity [PMID:10793139]
ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019]
EXISTENCE:genetic interaction:GO:0033262 regulation of nuclear cell cycle DNA replication [PMID:17542652]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11336698]
Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication
peptidylprolyl isomerase family protein CPR5
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8377189|PMID:11914276|PMID:26928762]
Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0016787 hydrolase activity [PMID:12028594]
EXISTENCE:direct assay:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:1653209]
EXISTENCE:mutant phenotype:GO:0009164 nucleoside catabolic process [PMID:9573184]
Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
EXISTENCE:curator inference:GO:0004842 ubiquitin-protein transferase activity [PMID:14747994]
EXISTENCE:curator inference:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:14747994]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:14747994]
F-box protein that regulates pheromone-dependent morphogenesis; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
putative dolichyl-phosphate-mannose--protein mannosyltransferase
Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:11118219]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:11118219]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:11056220]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:11118219]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11118219]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9367980]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:18387956]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:9367980]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:9367980]
EXISTENCE:genetic interaction:GO:0005886 plasma membrane [PMID:18387956]
EXISTENCE:genetic interaction:GO:0030010 establishment of cell polarity [PMID:9367980]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:16371506]
EXISTENCE:genetic interaction:GO:0032488 Cdc42 protein signal transduction [PMID:9367979]
EXISTENCE:mutant phenotype:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:18387956]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:14734533]
EXISTENCE:mutant phenotype:GO:0090338 positive regulation of formin-nucleated actin cable assembly [PMID:22918946]
EXISTENCE:physical interaction:GO:0031106 septin ring organization [PMID:16371506]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:9367979|PMID:9367980]
Rho-family GTPase interacting protein and Cdc42p effector; required with Gic1p for cell polarity establishment and initiation of budding; involved in recruitment of septins to the presumptive bud site; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; gic1 gic2 double mutants are defective for polarizing Cdc42p at elevated temperature, so may function both up- and downstream of Cdc42p; regulates mitotic exit
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12832469]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:12832469]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16077008]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8754829]
EXISTENCE:direct assay:GO:0010944 negative regulation of transcription by competitive promoter binding [PMID:12832469]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16077008]
EXISTENCE:genetic interaction:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:16077008]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10562556]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:12832469]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16077008]
EXISTENCE:mutant phenotype:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:16077008]
EXISTENCE:physical interaction:GO:0032298 positive regulation of DNA-templated DNA replication initiation [PMID:16077008]
Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
EXISTENCE:direct assay:GO:0000112 nucleotide-excision repair factor 3 complex [PMID:8855246]
EXISTENCE:direct assay:GO:0000439 transcription factor TFIIH core complex [PMID:14500720|PMID:19818408]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:15909982]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:15909982]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:7891722]
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
rRNA-binding ribosome biosynthesis protein
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12702244]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:11864606]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:11864606]
Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
phosphatidylinositol-3-phosphate-binding ubiquitin-protein ligase
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11526110]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:11526110]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11526110]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:11526110|PMID:31604822]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:9702203]
EXISTENCE:mutant phenotype:GO:0031647 regulation of protein stability [PMID:31604822]
EXISTENCE:mutant phenotype:GO:0042176 regulation of protein catabolic process [PMID:31604822]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:31604822]
RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol 3-phosphate; contains a FYVE finger domain
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:15916602|PMID:18936173]
Protein involved in nucleotide excision repair (NER); homologous to RAD4
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10390371]
EXISTENCE:direct assay:GO:0032958 inositol phosphate biosynthetic process [PMID:10960485]
EXISTENCE:direct assay:GO:0035299 inositol pentakisphosphate 2-kinase activity [PMID:10960485]
EXISTENCE:mutant phenotype:GO:0032958 inositol phosphate biosynthetic process [PMID:10960485|PMID:10390371]
EXISTENCE:mutant phenotype:GO:0035299 inositol pentakisphosphate 2-kinase activity [PMID:10960485]
EXISTENCE:mutant phenotype:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:17660569]
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable; human IPPK can complement ipk1 null mutant
EXISTENCE:direct assay:GO:0005740 mitochondrial envelope [PMID:15355998]
Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
YDRWTy1-4; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:15885712]
hypothetical protein involved in DNA repair
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:10323865]
EXISTENCE:direct assay:GO:0000817 COMA complex [PMID:14633972]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:26653857]
EXISTENCE:mutant phenotype:GO:1905262 negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination [PMID:26653857]
Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:29526591]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:22927826]
Protein needed for ER membrane biosynthesis in response to ER stress; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis; homologous to human FIT2/FITM2, a fatty acyl-CoA diphosphatase; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11553703]
EXISTENCE:direct assay:GO:0030276 clathrin binding [PMID:16687570]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:16687570]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:18948116]
EXISTENCE:direct assay:GO:0072318 clathrin coat disassembly [PMID:23913685]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:11553703]
EXISTENCE:mutant phenotype:GO:0072318 clathrin coat disassembly [PMID:11146663]
Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles; required for propagation of [URE3] prion variant
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:17537182|PMID:323221]
EXISTENCE:direct assay:GO:0004067 asparaginase activity [PMID:17537182|PMID:27824095]
EXISTENCE:mutant phenotype:GO:0004067 asparaginase activity [PMID:17537182|PMID:27824095]
EXISTENCE:mutant phenotype:GO:0006530 asparagine catabolic process [PMID:323221]
Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia; important enzyme for the treatment of acute lymphoblastic leukemia; has low glutaminase activity and dependence; synthesized constitutively
ARS452; Autonomously Replicating Sequence
mitochondrial 54S ribosomal protein YmL35
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552|PMID:9151978]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:28931599]
Mitochondrial ribosomal protein of the large subunit; involved together with Mrp7p in assembly of cytochrome c oxidase
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:9857174]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:19528297]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:16551625|PMID:20219971]
Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae
phosphatidylinositol-3-phosphate binding protein
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9168472|PMID:22842922]
EXISTENCE:direct assay:GO:0010009 cytoplasmic side of endosome membrane [PMID:9168472]
EXISTENCE:genetic interaction:GO:0006895 Golgi to endosome transport [PMID:9335586]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:1493335]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:9168472]
EXISTENCE:mutant phenotype:GO:0006904 vesicle docking involved in exocytosis [PMID:9168472]
EXISTENCE:mutant phenotype:GO:0006906 vesicle fusion [PMID:9168472]
EXISTENCE:physical interaction:GO:0006896 Golgi to vacuole transport [PMID:10021387]
Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:22588997|PMID:12150911]
EXISTENCE:direct assay:GO:0034455 t-UTP complex [PMID:17515605]
EXISTENCE:mutant phenotype:GO:0045943 positive regulation of transcription by RNA polymerase I [PMID:15489292]
Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript; Small Subunit processome is also known as SSU processome
EXISTENCE:direct assay:GO:0000796 condensin complex [PMID:10811823]
EXISTENCE:genetic interaction:GO:0003682 chromatin binding [PMID:11864994]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:11864994]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:11864994]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0010032 meiotic chromosome condensation [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0051307 meiotic chromosome separation [PMID:14662740]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:18708579]
EXISTENCE:mutant phenotype:GO:1903342 negative regulation of meiotic DNA double-strand break formation [PMID:25103240]
Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding by the complex; required for tRNA genes clustering at the nucleolus; required for replication slow zone breakage following Mec1p inactivation; transcription is cell cycle regulated, peaking in mitosis and declining in G1; protein is constitutively degraded by the proteasome; rate limiting for condensin recruitment to chromatin
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962064]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:26001273]
EXISTENCE:direct assay:GO:0032934 sterol binding [PMID:26001273]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:29339490]
EXISTENCE:mutant phenotype:GO:0015918 sterol transport [PMID:33255682]
EXISTENCE:mutant phenotype:GO:0032366 intracellular sterol transport [PMID:26001273]
Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:9346239]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11080155]
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:9346238|PMID:9346239]
EXISTENCE:direct assay:GO:0031518 CBF3 complex [PMID:8670864|PMID:8706132]
EXISTENCE:direct assay:GO:0051382 kinetochore assembly [PMID:12084919]
EXISTENCE:direct assay:GO:0065003 protein-containing complex assembly [PMID:15090617]
EXISTENCE:genetic interaction:GO:0007035 vacuolar acidification [PMID:11283612]
EXISTENCE:genetic interaction:GO:0031518 CBF3 complex [PMID:8706132]
EXISTENCE:mutant phenotype:GO:0000082 G1/S transition of mitotic cell cycle [PMID:8706131|PMID:8706132]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:8706131|PMID:8706132]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:16330709]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:11283612]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:17205042]
EXISTENCE:mutant phenotype:GO:0010458 exit from mitosis [PMID:17205042]
EXISTENCE:mutant phenotype:GO:0045116 protein neddylation [PMID:9531531]
EXISTENCE:mutant phenotype:GO:0051382 kinetochore assembly [PMID:12084919]
EXISTENCE:mutant phenotype:GO:2000766 negative regulation of cytoplasmic translation [PMID:26310304]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12769845]
EXISTENCE:physical interaction:GO:0007096 regulation of exit from mitosis [PMID:17205042]
EXISTENCE:physical interaction:GO:0031518 CBF3 complex [PMID:8670864]
EXISTENCE:physical interaction:GO:0043291 RAVE complex [PMID:11283612|PMID:11844802]
Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:23900285]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:1894692]
EXISTENCE:direct assay:GO:0032581 ER-dependent peroxisome organization [PMID:17646399]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:23900285]
EXISTENCE:mutant phenotype:GO:0045033 peroxisome inheritance [PMID:19948495]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:9418908]
Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:21211725]
UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8978684]
EXISTENCE:direct assay:GO:0042765 GPI-anchor transamidase complex [PMID:11598210]
EXISTENCE:genetic interaction:GO:0003923 GPI-anchor transamidase activity [PMID:10793132]
EXISTENCE:mutant phenotype:GO:0016255 attachment of GPI anchor to protein [PMID:11598210|PMID:10727241]
Catalytic subunit of the ER membrane GPI transamidase complex; involved in adding glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; luminally-oriented type I integral membrane glycoprotein of the ER; human PIGK protein is a functional homolog
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:32735705]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:25389272]
EXISTENCE:direct assay:GO:0036121 double-stranded DNA helicase activity [PMID:25389272]
EXISTENCE:direct assay:GO:0061749 forked DNA-dependent helicase activity [PMID:32735705]
EXISTENCE:mutant phenotype:GO:0032042 mitochondrial DNA metabolic process [PMID:25389272]
EXISTENCE:mutant phenotype:GO:0070125 mitochondrial translational elongation [PMID:34398681]
Double-stranded DNA-dependent helicase of the DExH/D-box family; branch point-binding helicase that preferentially unwinds the nascent lagging strand with a 3' to 5' polarity at a replication fork; contains double-stranded DNA translocase activity; responsible for DNA branch migration; required for maintenance of the mitochondrial (mt) genome; localizes to the mitochondrial matrix; monomeric
EXISTENCE:genetic interaction:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23479637]
EXISTENCE:mutant phenotype:GO:0072344 rescue of stalled ribosome [PMID:26943317]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23479637]
Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Rqc2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:31533921]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:16299513]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:14690608]
Swi2/Snf2-related ATPase; catalytic subunit of SWR1 complex, which exchanges histone variant H2A.Z (Htz1p) for chromatin-bound histone H2A; N-terminus can deliver H2A.Z-H2B dimer to DNA-(H3-H4)2 tetrasome; relocalizes to cytosol in response to hypoxia; chronological aging factor, mediates lifespan extension by dietary restriction
EXISTENCE:direct assay:GO:0005049 nuclear export signal receptor activity [PMID:9214382]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10485850|PMID:10545454|PMID:17020589]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17020589]
EXISTENCE:direct assay:GO:0006611 protein export from nucleus [PMID:12697814|PMID:9853758]
EXISTENCE:genetic interaction:GO:0071528 tRNA re-export from nucleus [PMID:20032305]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:23716718]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12697814]
Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog
Protein that associates with mitochondrial ribosome; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene
mitochondrial 37S ribosomal protein MRPS28
EXISTENCE:curator inference:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:30520011]
hypothetical protein; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
EXISTENCE:direct assay:GO:0004521 endoribonuclease activity [PMID:27034467]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16762320|PMID:16769905]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:16769905]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320|PMID:16769905]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320|PMID:16769905]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16762320|PMID:16769905]
EXISTENCE:mutant phenotype:GO:0004521 endoribonuclease activity [PMID:27034467]
EXISTENCE:physical interaction:GO:0032040 small-subunit processome [PMID:16769905]
PINc domain endonuclease; required for early cleavage of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
tRNA of undetermined specificity, predicted by tRNAscan-SE analysis; very similar to serine tRNAs
EXISTENCE:direct assay:GO:0004814 arginine-tRNA ligase activity [PMID:9622124]
EXISTENCE:direct assay:GO:0006420 arginyl-tRNA aminoacylation [PMID:9622124]
Arginyl-tRNA synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDR341C has a paralog, MSR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005355 glucose transmembrane transporter activity [PMID:12702270]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11904149|PMID:12469340|PMID:16622836]
EXISTENCE:direct assay:GO:1904659 glucose transmembrane transport [PMID:12702270]
EXISTENCE:mutant phenotype:GO:0005355 glucose transmembrane transporter activity [PMID:20525688]
EXISTENCE:mutant phenotype:GO:0015146 pentose transmembrane transporter activity [PMID:17180689]
High-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt6p, expressed at high basal levels relative to other HXTs, expression repressed by high glucose levels; HXT7 has a paralog, HXT4, that arose from the whole genome duplication
ARS432; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0031966 mitochondrial membrane [PMID:31862471]
High-affinity glucose transporter; member of the major facilitator superfamily, nearly identical to Hxt7p, expressed at high basal levels relative to other HXTs, repression of expression by high glucose requires SNF3; HXT6 has a paralog, HXT1, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22544065|PMID:24124599]
Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
ARS453; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:16034825]
Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
mitochondrial 37S ribosomal protein MRP1
Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:22190733]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:30679249]
hypothetical protein thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication
Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
Methionine tRNA (tRNA-Met), functions in translational elongation; not involved in translational initiation; predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12646576]
EXISTENCE:genetic interaction:GO:0045182 translation regulator activity [PMID:17110482]
EXISTENCE:genetic interaction:GO:0070131 positive regulation of mitochondrial translation [PMID:17110482]
EXISTENCE:mutant phenotype:GO:0045182 translation regulator activity [PMID:17110482]
EXISTENCE:mutant phenotype:GO:0070071 proton-transporting two-sector ATPase complex assembly [PMID:17110482]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:17110482]
Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:10679005]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:10679005]
Protein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23169667|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:23169667]
EXISTENCE:mutant phenotype:GO:0034488 basic amino acid transmembrane export from vacuole [PMID:23169667]
EXISTENCE:mutant phenotype:GO:0034490 basic amino acid transmembrane import into vacuole [PMID:32776922]
Putative vacuolar membrane transporter for cationic amino acids; involved in vacuolar export of arginine under nitrogen starvation conditions; appears to be inactive under nitrogen replete conditions; may contribute to amino acid homeostasis; vacuolar membrane protein; member of the PQ-loop family, containing seven transmembrane domains; homologous to the human lysosomal cationic amino acid exporter PQLC2; null mutant is functionally complemented by the rat PQLC2 vacuolar transporter
EXISTENCE:direct assay:GO:0004791 thioredoxin-disulfide reductase activity [PMID:7961686]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18406344]
EXISTENCE:direct assay:GO:0008198 ferrous iron binding [PMID:18406344]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:9571241]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:15051715]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:9571241]
Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004048 anthranilate phosphoribosyltransferase activity [PMID:2428012]
EXISTENCE:mutant phenotype:GO:0000162 tryptophan biosynthetic process [PMID:10790693]
Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:8886974|PMID:8247006]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762|PMID:8886974]
EXISTENCE:direct assay:GO:0005822 inner plaque of spindle pole body [PMID:9215630|PMID:28331072]
EXISTENCE:direct assay:GO:0005823 central plaque of spindle pole body [PMID:9215630|PMID:8886974]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:9384578|PMID:24842996]
EXISTENCE:direct assay:GO:0065003 protein-containing complex assembly [PMID:27226487|PMID:24842996|PMID:20631709]
EXISTENCE:direct assay:GO:0110120 gamma-tubulin complex localization to nuclear side of mitotic spindle pole body [PMID:9384578]
EXISTENCE:genetic interaction:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12836012]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:23468650]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12836012]
EXISTENCE:mutant phenotype:GO:0005200 structural constituent of cytoskeleton [PMID:7503995]
EXISTENCE:mutant phenotype:GO:0044877 protein-containing complex binding [PMID:24842996]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:24842996]
EXISTENCE:mutant phenotype:GO:0090063 positive regulation of microtubule nucleation [PMID:24842996]
EXISTENCE:mutant phenotype:GO:1902440 protein localization to mitotic spindle pole body [PMID:8886974]
EXISTENCE:physical interaction:GO:0110120 gamma-tubulin complex localization to nuclear side of mitotic spindle pole body [PMID:9384578]
Inner plaque spindle pole body (SPB) component; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner; ortholog of human pericentrin (kendrin)
EXISTENCE:direct assay:GO:0031083 BLOC-1 complex [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0007032 endosome organization [PMID:23547030]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:23547030]
Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:10747088]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:15039776|PMID:20150893]
EXISTENCE:genetic interaction:GO:0006896 Golgi to vacuole transport [PMID:10747088]
EXISTENCE:mutant phenotype:GO:0006895 Golgi to endosome transport [PMID:15039776]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:10747088]
EXISTENCE:mutant phenotype:GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15039776]
Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:16135807]
EXISTENCE:direct assay:GO:0035267 NuA4 histone acetyltransferase complex [PMID:15485911|PMID:15353583]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:18212047]
Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:27913624]
EXISTENCE:mutant phenotype:GO:0000055 ribosomal large subunit export from nucleus [PMID:12912920]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:12912920]
Essential nuclear chaperone for ribosomal protein Rpl23p; involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
transcription factor TFIIIC subunit TFC6
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:9418847|PMID:1474578]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:2183033|PMID:3633923|PMID:8464480|PMID:9418847]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:genetic interaction:GO:0006383 transcription by RNA polymerase III [PMID:9418847]
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
ETC6; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; remains predominantly localized to the nuclear periphery throughout cell cycle; located between divergently transcribed genes TFC6 and ESC2
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11090616]
EXISTENCE:direct assay:GO:0033235 positive regulation of protein sumoylation [PMID:33446573]
EXISTENCE:direct assay:GO:0061656 SUMO conjugating enzyme activity [PMID:33446573]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:19158388]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:18757937]
EXISTENCE:genetic interaction:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:19158388]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:19158388]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:18757937]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11090616]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:19158388]
EXISTENCE:mutant phenotype:GO:0033235 positive regulation of protein sumoylation [PMID:23935535]
Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
proteasome regulatory particle lid subunit SEM1
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9927667]
EXISTENCE:direct assay:GO:0008541 proteasome regulatory particle, lid subcomplex [PMID:15117943|PMID:15572408]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:15117943]
EXISTENCE:direct assay:GO:0070390 transcription export complex 2 [PMID:19289793]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:19061648]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:19061648]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:15572408]
EXISTENCE:mutant phenotype:GO:0016578 histone deubiquitination [PMID:23599000]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:9927667]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:23620289]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:15117943]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:19217412]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:10373512]
EXISTENCE:mutant phenotype:GO:0072742 SAGA complex localization to transcription regulatory region [PMID:23599000]
EXISTENCE:physical interaction:GO:0006406 mRNA export from nucleus [PMID:19061648]
19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005681 spliceosomal complex [PMID:9769104]
EXISTENCE:genetic interaction:GO:0000348 mRNA branch site recognition [PMID:15133121]
EXISTENCE:genetic interaction:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:10999606]
EXISTENCE:genetic interaction:GO:0000389 mRNA 3'-splice site recognition [PMID:10628969|PMID:15133121]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:10999606]
EXISTENCE:mutant phenotype:GO:0000386 second spliceosomal transesterification activity [PMID:10999606]
Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression, particularly at the G1/S phase transition; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functionally complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15056729]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:15056729]
Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YDRWTy1-5; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:19726565]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14690591]
EXISTENCE:mutant phenotype:GO:0006673 inositol phosphoceramide metabolic process [PMID:19726565]
EXISTENCE:mutant phenotype:GO:0070917 inositol phosphoceramide synthase regulator activity [PMID:19726565]
EXISTENCE:physical interaction:GO:0070916 inositol phosphoceramide synthase complex [PMID:19726565]
Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
trifunctional aldehyde reductase/carbonyl reductase (NADPH)/glucose 1-dehydrogenase (NADP(+)) YPR1
EXISTENCE:direct assay:GO:0004090 carbonyl reductase (NADPH) activity [PMID:19016485]
EXISTENCE:direct assay:GO:0019568 arabinose catabolic process [PMID:12271459]
EXISTENCE:direct assay:GO:0042843 D-xylose catabolic process [PMID:12271459]
EXISTENCE:mutant phenotype:GO:0019568 arabinose catabolic process [PMID:12271459]
NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:14522986]
EXISTENCE:direct assay:GO:0003691 double-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9845372]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:14522986]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:15721260]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:17698079]
EXISTENCE:direct assay:GO:0051880 G-quadruplex DNA binding [PMID:17698079]
EXISTENCE:genetic interaction:GO:0006284 base-excision repair [PMID:20040573]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:15548595|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0006284 base-excision repair [PMID:20040573]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:12399380|PMID:9501103]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:15548595]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:27173583]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:15548595]
EXISTENCE:mutant phenotype:GO:0097552 mitochondrial double-strand break repair via homologous recombination [PMID:22214610]
EXISTENCE:physical interaction:GO:0030870 Mre11 complex [PMID:9845372]
FHA domain-containing component of the Mre11 complex; MRE11-RAD50-XRS2 complex is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling; FHA domain is required for activation of Tel1p
EXISTENCE:direct assay:GO:0110155 NAD-cap decapping [PMID:33139726]
EXISTENCE:genetic interaction:GO:0004534 5'-3' exoribonuclease activity [PMID:35140172]
EXISTENCE:genetic interaction:GO:0070966 nuclear-transcribed mRNA catabolic process, no-go decay [PMID:31913314]
EXISTENCE:mutant phenotype:GO:0000470 maturation of LSU-rRNA [PMID:35140172]
EXISTENCE:mutant phenotype:GO:0004534 5'-3' exoribonuclease activity [PMID:35140172]
EXISTENCE:mutant phenotype:GO:0110155 NAD-cap decapping [PMID:33139726]
Cytoplasmic 5' exoribonuclease; transcriptomics wide distributive exonuclease; involved in cytoplasmic mRNA degradation, turnover of tRNA introns and processing of spliceosomal introns; required for the final 5'-end processing of 25S rRNA; minor contributor to No-Go Decay and the unfolded protein response; decapping protein involved in surveillance and removal of non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs); sequence similarity to Rai1p
EXISTENCE:genetic interaction:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:18809304]
Putative chitinase; functionally complements A. gossypii cts2 mutant sporulation defect
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:18410729]
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:22553352]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:18410729]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:20026658]
EXISTENCE:genetic interaction:GO:0045053 protein retention in Golgi apparatus [PMID:32673164]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:18635803]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16582425]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:32673164|PMID:18410729]
EXISTENCE:mutant phenotype:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:22553352]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:20026658]
EXISTENCE:physical interaction:GO:0019899 enzyme binding [PMID:18410729]
Golgi PI4K effector, PtdIns4P sensor, and retention receptor; interacts with glycosyltransferases, and in the PtdIns4P-bound state mediates retention of these enzymes in the Golgi; PtdIns4P sensor that limits PI4K signaling and regulates lipid homeostasis, interacting with the catalytic domain of Sac1p, the major PtdIns4P phosphatase, directing dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; contributes to telomere function; ortholog of human GOLPH3
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:17908202]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:10559922]
EXISTENCE:mutant phenotype:GO:0009966 regulation of signal transduction [PMID:12477731]
EXISTENCE:mutant phenotype:GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity [PMID:10559922]
N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functionally complements the heat sensitivity of a frq1 ts mutant
mRNA-binding phosphate metabolism regulator
EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR binding [PMID:24206186]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24206186]
EXISTENCE:mutant phenotype:GO:0019220 regulation of phosphate metabolic process [PMID:24206186]
EXISTENCE:mutant phenotype:GO:0043488 regulation of mRNA stability [PMID:24206186]
N6-Methyladenosine (m6A) reader; co-transcriptionally recruited to specific methylated mRNAs during meiotic prophase, contributing to the down-regulation of transcript abundance, and the timing of meiotic recombination; regulates PHO4 mRNA stability, binding to the 3'UTR in a phosphate-dependent manner; posttranscriptional regulator of phosphate and glucose metabolism; contains a highly conserved YTH domain that exhibits RNA-binding activity; human homolog YTHDF2 can complement yeast null mutant
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:22561346]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:22561346]
EXISTENCE:genetic interaction:GO:0007059 chromosome segregation [PMID:22561346]
Kinetochore localized hypothetical protein; interacts with Cnn1p (CENP-T); orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-W and fission yeast new1
bifunctional AAA family ATPase chaperone/translocase BCS1
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:8599931]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:22017868]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:22017868]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:22017868]
EXISTENCE:mutant phenotype:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:24055101]
EXISTENCE:mutant phenotype:GO:0051131 chaperone-mediated protein complex assembly [PMID:10508156]
EXISTENCE:physical interaction:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:22017868]
Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane and delivers it to an assembly intermediate of respiratory Complex III; also required for assembly of the Qcr10p subunit; mutation is functionally complemented by human homolog BCS1L, linked to neonatal diseases
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9727014]
EXISTENCE:direct assay:GO:0015039 NADPH-adrenodoxin reductase activity [PMID:9727014]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:20534343]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:11035018]
Oxidoreductase of the mitochondrial inner membrane; involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
EXISTENCE:mutant phenotype:GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) [PMID:9288937]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:9288937]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:9288937]
Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18611963]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15075370|PMID:10369684]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:10369684|PMID:11720284]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15075370]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10428970]
EXISTENCE:mutant phenotype:GO:0030490 maturation of SSU-rRNA [PMID:19806183]
EXISTENCE:physical interaction:GO:0005732 sno(s)RNA-containing ribonucleoprotein complex [PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12455995|PMID:17981141]
EXISTENCE:direct assay:GO:0032177 cellular bud neck split septin rings [PMID:18166650]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
EXISTENCE:genetic interaction:GO:0005096 GTPase activator activity [PMID:12455995]
EXISTENCE:genetic interaction:GO:0031106 septin ring organization [PMID:14517318|PMID:12455995]
EXISTENCE:genetic interaction:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
EXISTENCE:mutant phenotype:GO:0007264 small GTPase mediated signal transduction [PMID:12455995]
EXISTENCE:mutant phenotype:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
EXISTENCE:physical interaction:GO:0043547 positive regulation of GTPase activity [PMID:12455995]
GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0034553 mitochondrial respiratory chain complex II assembly [PMID:19465911]
EXISTENCE:physical interaction:GO:0034553 mitochondrial respiratory chain complex II assembly [PMID:24954417]
Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; member of the LYR protein family; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy
EXISTENCE:direct assay:GO:0006559 L-phenylalanine catabolic process [PMID:12902239]
EXISTENCE:direct assay:GO:0050177 phenylpyruvate decarboxylase activity [PMID:12902239|PMID:21501384]
EXISTENCE:genetic interaction:GO:0000949 aromatic amino acid family catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:genetic interaction:GO:0000950 branched-chain amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006552 leucine catabolic process [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006559 L-phenylalanine catabolic process [PMID:12499363]
EXISTENCE:genetic interaction:GO:0006569 tryptophan catabolic process [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0000951 methionine catabolic process to 3-methylthiopropanol [PMID:16423070]
EXISTENCE:mutant phenotype:GO:0006552 leucine catabolic process [PMID:10753893]
EXISTENCE:mutant phenotype:GO:0016831 carboxy-lyase activity [PMID:10753893]
Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:11390651]
EXISTENCE:direct assay:GO:0043462 regulation of ATP-dependent activity [PMID:28059701]
EXISTENCE:direct assay:GO:0071667 DNA/RNA hybrid binding [PMID:29954833]
EXISTENCE:direct assay:GO:1902281 negative regulation of RNA helicase activity [PMID:23721653]
EXISTENCE:direct assay:GO:1990119 RNA helicase inhibitor activity [PMID:23721653]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:11979277]
Nuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:28794267]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:28794267]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:28794267]
EXISTENCE:mutant phenotype:GO:0097250 mitochondrial respirasome assembly [PMID:28794267]
Mitochondrial assembly factor; important for assembly of the mitochondrial respiratory chain bc1 complex (complex III), cytochrome oxidase (complex IV), and the complex III-complex IV supercomplex; embedded in the mitochondrial inner membrane, facing the intermembrane space
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:9559554]
Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:12408861]
Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4
ARS433; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12966084]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:12429834]
Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:16950777]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:10835368|PMID:16950777]
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:30876804]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:16950777]
EXISTENCE:mutant phenotype:GO:0045901 positive regulation of translational elongation [PMID:19338753]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:16950777]
Eukaryotic translation elongation factor 2 (eEF2, EF-2); also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004520 endodeoxyribonuclease activity [PMID:11641278]
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:25765656]
EXISTENCE:direct assay:GO:0008821 crossover junction endodeoxyribonuclease activity [PMID:17363897]
EXISTENCE:direct assay:GO:0048476 Holliday junction resolvase complex [PMID:17363897]
EXISTENCE:direct assay:GO:0051097 negative regulation of helicase activity [PMID:25765656]
EXISTENCE:genetic interaction:GO:0000712 resolution of meiotic recombination intermediates [PMID:18691964]
EXISTENCE:genetic interaction:GO:0000727 double-strand break repair via break-induced replication [PMID:23071463]
EXISTENCE:genetic interaction:GO:0006265 DNA topological change [PMID:11139495]
EXISTENCE:genetic interaction:GO:0007131 reciprocal meiotic recombination [PMID:11641278]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:23071463]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:10905349]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495]
Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p
Putative transporter; member of the sugar porter family; non-essential gene; overexpression results in elevated colony sectoring, an indicator of chromosomal instability
EXISTENCE:direct assay:GO:0005516 calmodulin binding [PMID:27093085]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:32015451]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20610658|PMID:27093085]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10393809|PMID:16239147|PMID:16824951]
EXISTENCE:direct assay:GO:0042803 protein homodimerization activity [PMID:32015451]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:10393809|PMID:19053807]
EXISTENCE:direct assay:GO:0060988 lipid tube assembly [PMID:20610658]
EXISTENCE:direct assay:GO:0097320 plasma membrane tubulation [PMID:27093085]
EXISTENCE:genetic interaction:GO:0072741 protein localization to cell division site [PMID:23469085]
EXISTENCE:genetic interaction:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:23469085]
EXISTENCE:mutant phenotype:GO:0005516 calmodulin binding [PMID:27093085]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:27093085|PMID:8590801]
EXISTENCE:mutant phenotype:GO:0008289 lipid binding [PMID:27093085]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:8590801]
EXISTENCE:mutant phenotype:GO:0097320 plasma membrane tubulation [PMID:27093085]
EXISTENCE:mutant phenotype:GO:1903475 mitotic actomyosin contractile ring assembly [PMID:23469085]
EXISTENCE:physical interaction:GO:0008092 cytoskeletal protein binding [PMID:10388809]
EXISTENCE:physical interaction:GO:0016192 vesicle-mediated transport [PMID:15802519]
EXISTENCE:physical interaction:GO:1990528 Rvs161p-Rvs167p complex [PMID:20610658]
Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin; necessary for efficient trafficking of CoQ6 to mitochondria
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:9038344]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:23878277]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:9038344]
EXISTENCE:genetic interaction:GO:0032956 regulation of actin cytoskeleton organization [PMID:2183030|PMID:9038344]
EXISTENCE:genetic interaction:GO:0035024 negative regulation of Rho protein signal transduction [PMID:9038344]
EXISTENCE:genetic interaction:GO:0060237 regulation of fungal-type cell wall organization [PMID:12949174|PMID:10625705]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:10625705]
EXISTENCE:mutant phenotype:GO:0090038 negative regulation of protein kinase C signaling [PMID:10625705|PMID:12207708]
GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7629121]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:9312010]
EXISTENCE:direct assay:GO:0019948 SUMO activating enzyme activity [PMID:9312010]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:9312010]
EXISTENCE:mutant phenotype:GO:0019948 SUMO activating enzyme activity [PMID:9312010]
EXISTENCE:physical interaction:GO:0031510 SUMO activating enzyme complex [PMID:9312010]
Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in glycine codons
hypothetical protein; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9224714|PMID:9674426]
EXISTENCE:direct assay:GO:0001403 invasive growth in response to glucose limitation [PMID:12072450]
EXISTENCE:direct assay:GO:0003712 transcription coregulator activity [PMID:11485989]
EXISTENCE:direct assay:GO:0007124 pseudohyphal growth [PMID:12072450]
EXISTENCE:mutant phenotype:GO:0003712 transcription coregulator activity [PMID:9858534]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:12370284]
Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:12591915]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:11907266]
EXISTENCE:mutant phenotype:GO:0007007 inner mitochondrial membrane organization [PMID:12591915]
Protein required for normal mitochondrial morphology; localized to mitochondrial inner membrane; may be involved in fission of the inner membrane; forms a homo-oligomeric complex
proteasome regulatory particle base subunit RPT3
EXISTENCE:direct assay:GO:0008540 proteasome regulatory particle, base subcomplex [PMID:11742986|PMID:9741626]
EXISTENCE:mutant phenotype:GO:0045899 positive regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:19843524]
ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9238021]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9238021]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:9412461]
EXISTENCE:genetic interaction:GO:0006406 mRNA export from nucleus [PMID:9238021]
EXISTENCE:mutant phenotype:GO:0061608 nuclear import signal receptor activity [PMID:9412461]
EXISTENCE:physical interaction:GO:0061608 nuclear import signal receptor activity [PMID:9412461]
Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
negative cofactor 2 transcription regulator complex subunit NCB2
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:8948634|PMID:9023340|PMID:9096360]
EXISTENCE:direct assay:GO:0001046 core promoter sequence-specific DNA binding [PMID:18703679]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:18703679]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:8948634]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9023340]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:21976730]
EXISTENCE:direct assay:GO:0017054 negative cofactor 2 complex [PMID:8948634]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8948634]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10713169]
EXISTENCE:genetic interaction:GO:0003714 transcription corepressor activity [PMID:10713169]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10713169|PMID:9023340]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:10713169|PMID:9023340]
EXISTENCE:mutant phenotype:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:9023340]
EXISTENCE:mutant phenotype:GO:0045898 regulation of RNA polymerase II transcription preinitiation complex assembly [PMID:21976730]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18048413]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:18048413]
EXISTENCE:physical interaction:GO:0017025 TBP-class protein binding [PMID:9096360]
EXISTENCE:physical interaction:GO:0017054 negative cofactor 2 complex [PMID:9023340]
Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p
EXISTENCE:direct assay:GO:0033553 rDNA heterochromatin [PMID:15489292]
Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
EXISTENCE:direct assay:GO:0004422 hypoxanthine phosphoribosyltransferase activity [PMID:371963|PMID:6170313|PMID:7035445]
EXISTENCE:direct assay:GO:0032263 GMP salvage [PMID:7035445]
EXISTENCE:direct assay:GO:0032264 IMP salvage [PMID:3546595|PMID:7035445]
EXISTENCE:genetic interaction:GO:0004422 hypoxanthine phosphoribosyltransferase activity [PMID:9335580]
EXISTENCE:genetic interaction:GO:0032263 GMP salvage [PMID:20980241]
EXISTENCE:mutant phenotype:GO:0004422 hypoxanthine phosphoribosyltransferase activity [PMID:9335580]
EXISTENCE:mutant phenotype:GO:0032263 GMP salvage [PMID:20980241]
Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
trifunctional uridine nucleosidase/nicotinamide riboside hydrolase/nicotinic acid riboside hydrolase
EXISTENCE:direct assay:GO:0045437 uridine nucleosidase activity [PMID:19001417]
EXISTENCE:direct assay:GO:0046135 pyrimidine nucleoside catabolic process [PMID:11872485|PMID:12111094]
EXISTENCE:direct assay:GO:0050263 ribosylpyrimidine nucleosidase activity [PMID:11872485|PMID:12111094]
EXISTENCE:direct assay:GO:0070635 nicotinamide riboside hydrolase activity [PMID:19001417]
EXISTENCE:direct assay:GO:0070636 nicotinic acid riboside hydrolase activity [PMID:19001417]
EXISTENCE:genetic interaction:GO:0019358 nicotinate nucleotide salvage [PMID:17482543]
EXISTENCE:genetic interaction:GO:0034356 NAD biosynthesis via nicotinamide riboside salvage pathway [PMID:19001417]
EXISTENCE:mutant phenotype:GO:0006216 cytidine catabolic process [PMID:23670538]
EXISTENCE:mutant phenotype:GO:0006218 uridine catabolic process [PMID:23670538]
EXISTENCE:mutant phenotype:GO:0008655 pyrimidine-containing compound salvage [PMID:11872485]
Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
EXISTENCE:direct assay:GO:0003959 NADPH dehydrogenase activity [PMID:29365103]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:29365103]
EXISTENCE:direct assay:GO:0030476 ascospore wall assembly [PMID:8183942]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:8183942]
N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:direct assay:GO:0003824 catalytic activity [PMID:8183942]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:32503197]
Sporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
ARS434; Autonomously Replicating Sequence
DNA-directed RNA polymerase II subunit RPB7
EXISTENCE:direct assay:GO:0000932 P-body [PMID:17875743]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:11087726]
EXISTENCE:direct assay:GO:0003727 single-stranded RNA binding [PMID:11087726]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17056745]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:2183013|PMID:2186966|PMID:1331084]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17056745]
EXISTENCE:direct assay:GO:0006367 transcription initiation at RNA polymerase II promoter [PMID:11087726]
EXISTENCE:genetic interaction:GO:0000291 nuclear-transcribed mRNA catabolic process, exonucleolytic [PMID:17875743]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:10082533]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:11087726]
EXISTENCE:mutant phenotype:GO:0003727 single-stranded RNA binding [PMID:11087726]
EXISTENCE:mutant phenotype:GO:0045948 positive regulation of translational initiation [PMID:21074047]
EXISTENCE:mutant phenotype:GO:0060213 positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:17875743]
EXISTENCE:physical interaction:GO:0031369 translation initiation factor binding [PMID:21074047]
RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
mitochondrial 54S ribosomal protein YmL41
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:1544898|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:1544898|PMID:9151978]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:1544898]
ATP-binding cassette multidrug transporter PDR15
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:17881724]
Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:16314430]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16314430]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17041589]
EXISTENCE:mutant phenotype:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:11239471]
EXISTENCE:mutant phenotype:GO:0034498 early endosome to Golgi transport [PMID:16314430]
EXISTENCE:mutant phenotype:GO:0043087 regulation of GTPase activity [PMID:17041589]
Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
phosphoribosylglycinamide formyltransferase
EXISTENCE:genetic interaction:GO:0004644 phosphoribosylglycinamide formyltransferase activity [PMID:4581491]
EXISTENCE:mutant phenotype:GO:0004644 phosphoribosylglycinamide formyltransferase activity [PMID:4581491]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:4581491]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:4581491]
EXISTENCE:mutant phenotype:GO:0046084 adenine biosynthetic process [PMID:17247984]
Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
ETC1; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; located between divergently transcribed genes ADE8 and SIZ1
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:16204216]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:18701921]
EXISTENCE:direct assay:GO:0005940 septin ring [PMID:11587849]
EXISTENCE:direct assay:GO:0016925 protein sumoylation [PMID:23443663|PMID:26960795]
EXISTENCE:direct assay:GO:0019789 SUMO transferase activity [PMID:11577116|PMID:26960795]
EXISTENCE:direct assay:GO:0031397 negative regulation of protein ubiquitination [PMID:23443663]
EXISTENCE:genetic interaction:GO:0016925 protein sumoylation [PMID:26960795]
EXISTENCE:genetic interaction:GO:1990683 DNA double-strand break attachment to nuclear envelope [PMID:27056668]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16204216]
EXISTENCE:mutant phenotype:GO:0016925 protein sumoylation [PMID:11587849]
EXISTENCE:mutant phenotype:GO:0019789 SUMO transferase activity [PMID:11577116|PMID:11587849]
EXISTENCE:mutant phenotype:GO:0031397 negative regulation of protein ubiquitination [PMID:23443663]
SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p
protein-S-isoprenylcysteine carboxyl O-methyltransferase
EXISTENCE:direct assay:GO:0004671 protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity [PMID:2050108]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11451995|PMID:9693378]
EXISTENCE:mutant phenotype:GO:0004671 protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity [PMID:11451995|PMID:8212897]
EXISTENCE:mutant phenotype:GO:0005637 nuclear inner membrane [PMID:18923140]
EXISTENCE:mutant phenotype:GO:0006481 C-terminal protein methylation [PMID:2173693]
EXISTENCE:mutant phenotype:GO:0007323 peptide pheromone maturation [PMID:2050108]
Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762|PMID:15093775]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:20579315]
EXISTENCE:genetic interaction:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:17083136]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:20579315]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:17083136]
EXISTENCE:mutant phenotype:GO:1904152 regulation of retrograde protein transport, ER to cytosol [PMID:29351849]
Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
EXISTENCE:direct assay:GO:0004534 5'-3' exoribonuclease activity [PMID:20005841]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:20005841]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:20005841]
EXISTENCE:direct assay:GO:1990275 preribosome binding [PMID:20005841]
EXISTENCE:mutant phenotype:GO:0000477 generation of mature 5'-end of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:20005841]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12837249]
5' exoribonuclease required for 5' end processing of pre-60S rRNA; binds late pre-60S ribosomes, accompanying them from nucleolus to nuclear periphery; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; yeast null mutant is complemented by expression of human homolog NOL12
EXISTENCE:direct assay:GO:0016020 membrane [PMID:2178921]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:2178921|PMID:34821548]
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:2178921]
Predicted membrane protein required for lumenal ER protein retention; recycles inorganic phosphate byproduct of glycosylation from ER and Golgi; involved in recycling of early Golgi glycosyltransferases from late to early Golgi compartments; mutants secrete the endogenous ER protein, BiP (Kar2p)
Putative aminopeptidase; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:11105756|PMID:11842115]
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:11105756]
EXISTENCE:direct assay:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:19617314]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:11842115]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:11105756|PMID:11842115]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:11105756]
EXISTENCE:direct assay:GO:0071008 U2-type post-mRNA release spliceosomal complex [PMID:11105756]
Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:9822666]
Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
ARS435; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:10601233|PMID:9974380]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23313845]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:23313845]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:10713149]
EXISTENCE:direct assay:GO:0070987 error-free translesion synthesis [PMID:10713149|PMID:10932195|PMID:11027270|PMID:9974380]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:23326240]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:23326240]
EXISTENCE:mutant phenotype:GO:0042276 error-prone translesion synthesis [PMID:12110599|PMID:16387871]
EXISTENCE:physical interaction:GO:0005657 replication fork [PMID:11545742]
DNA polymerase eta; involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have a role in protection against mitochondrial mutagenesis; may be involved in meiosis; mutations in human pol eta are responsible for XPV
ARS436; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8550469]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:17627274]
EXISTENCE:genetic interaction:GO:0005034 osmosensor activity [PMID:17627274]
EXISTENCE:genetic interaction:GO:0006972 hyperosmotic response [PMID:17627274]
EXISTENCE:genetic interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:17627274]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:8550469]
EXISTENCE:mutant phenotype:GO:0006075 (1->3)-beta-D-glucan biosynthetic process [PMID:8550469]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:8550469]
EXISTENCE:physical interaction:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:17627274]
Mucin family member that functions as an osmosensor in the HOG pathway; large, highly glycosylated protein that functions redundantly with Msb2p in Sho1p-mediated osmostresss induction of the HOG signaling pathway; cytoplasmic domain interacts with Ahk1p a scaffold protein that prevents cross talk with the Kss1p MAPK of the filamentous growth pathway; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:23651256]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23651256|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0009074 aromatic amino acid family catabolic process [PMID:10207060]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10207060]
Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:11331606]
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:14966266]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:14966266]
EXISTENCE:direct assay:GO:0031588 nucleotide-activated protein kinase complex [PMID:12393914]
EXISTENCE:genetic interaction:GO:0004679 AMP-activated protein kinase activity [PMID:10990457]
EXISTENCE:genetic interaction:GO:0007165 signal transduction [PMID:10990457]
EXISTENCE:genetic interaction:GO:0043254 regulation of protein-containing complex assembly [PMID:9121458]
EXISTENCE:genetic interaction:GO:0140767 enzyme-substrate adaptor activity [PMID:27592031]
EXISTENCE:mutant phenotype:GO:0004679 AMP-activated protein kinase activity [PMID:12393914]
EXISTENCE:physical interaction:GO:0031588 nucleotide-activated protein kinase complex [PMID:1496382|PMID:7813428|PMID:9121458]
One of three alternate beta-subunits of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9372930]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15341652|PMID:17187783]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15341652|PMID:17187783]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9372930]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9372930]
AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
One of two upstream open reading frames (uORFs) in 5' untranslated region of CAD1 gene, regulate translation
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:17546040]
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:21633107]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:26928762]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:22213798]
EXISTENCE:genetic interaction:GO:0008574 plus-end-directed microtubule motor activity [PMID:23761070]
EXISTENCE:genetic interaction:GO:0051292 nuclear pore complex assembly [PMID:17546040]
EXISTENCE:mutant phenotype:GO:0008574 plus-end-directed microtubule motor activity [PMID:21633107|PMID:23761070]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:22213798]
EXISTENCE:mutant phenotype:GO:0040001 establishment of mitotic spindle localization [PMID:21633107|PMID:22213798]
EXISTENCE:mutant phenotype:GO:0051292 nuclear pore complex assembly [PMID:17546040]
EXISTENCE:physical interaction:GO:0005868 cytoplasmic dynein complex [PMID:21633107]
EXISTENCE:physical interaction:GO:0051292 nuclear pore complex assembly [PMID:17546040]
Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:direct assay:GO:0035091 phosphatidylinositol binding [PMID:11557775]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:12554655]
Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
proteasome regulatory particle lid subunit RPN9
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:10490597]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:10490597]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:10490597]
Non-ATPase regulatory subunit of the 26S proteasome; similar to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0004061 arylformamidase activity [PMID:18205391]
EXISTENCE:direct assay:GO:0009435 NAD biosynthetic process [PMID:18205391]
Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
translation initiation factor eIF3 core subunit g
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:9671501]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9671501|PMID:9660829]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:9671501]
EXISTENCE:direct assay:GO:0032781 positive regulation of ATP-dependent activity [PMID:29192585]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:15838098]
EXISTENCE:mutant phenotype:GO:0002188 translation reinitiation [PMID:20679478]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:25925566]
EXISTENCE:physical interaction:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9660829]
eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:15606766]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:15772085]
EXISTENCE:genetic interaction:GO:0034982 mitochondrial protein processing [PMID:28228553]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:15772085]
EXISTENCE:mutant phenotype:GO:0051603 proteolysis involved in protein catabolic process [PMID:15772085]
Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology; mutation in human homolog PITRM1 is implicated in autosomal recessive spinocerebellar ataxias (ARCA)
EXISTENCE:direct assay:GO:0003691 double-stranded telomeric DNA binding [PMID:32026548]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:17684230|PMID:7774613|PMID:23222640]
EXISTENCE:direct assay:GO:0008143 poly(A) binding [PMID:7962083]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:22284680]
EXISTENCE:direct assay:GO:0031370 eukaryotic initiation factor 4G binding [PMID:22284680]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:18818768]
EXISTENCE:direct assay:GO:0061752 telomeric repeat-containing RNA binding [PMID:32026548]
EXISTENCE:direct assay:GO:2000805 negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled [PMID:15902270]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:19061647]
EXISTENCE:genetic interaction:GO:0006415 translational termination [PMID:19733178]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:19061647]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:8675010]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:8675010]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:19733178]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:18818768]
EXISTENCE:mutant phenotype:GO:2000805 negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled [PMID:15902270]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:18818768]
RNA-binding protein; promotes elongation, regulates termination, and is involved in nuclear export of poly(A) mRNA; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ligase Bre1p, linking histone ubiquitination to mRNA processing; binds TERRA and stabilizes R-loops at short telomeres; phosphorylation by Sky1p and interaction with Mtr10p promotes shuttling and mRNA dissociation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15075373|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:15939668]
EXISTENCE:direct assay:GO:0042765 GPI-anchor transamidase complex [PMID:15939668]
EXISTENCE:mutant phenotype:GO:0016255 attachment of GPI anchor to protein [PMID:11483512]
Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
EXISTENCE:mutant phenotype:GO:0006481 C-terminal protein methylation [PMID:11060018]
EXISTENCE:mutant phenotype:GO:0010506 regulation of autophagy [PMID:23870128]
EXISTENCE:mutant phenotype:GO:0018423 protein C-terminal leucine carboxyl O-methyltransferase activity [PMID:11060018]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:11060018]
Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth
EXISTENCE:mutant phenotype:GO:0006883 cellular sodium ion homeostasis [PMID:7768897]
Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
phosphatidylinositol N-acetylglucosaminyltransferase GPI19
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:14690591|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16278447]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:16278447]
EXISTENCE:mutant phenotype:GO:0008194 UDP-glycosyltransferase activity [PMID:16278447]
EXISTENCE:physical interaction:GO:0000506 glycosylphosphatidylinositol-N- acetylglucosaminyltransferase (GPI-GnT) complex [PMID:16278447]
Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16850348]
Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension
EXISTENCE:direct assay:GO:0000183 rDNA heterochromatin formation [PMID:10082585]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10082585]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12689592]
EXISTENCE:direct assay:GO:0033551 monopolin complex [PMID:12689592]
EXISTENCE:genetic interaction:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:12689592]
EXISTENCE:mutant phenotype:GO:0045143 homologous chromosome segregation [PMID:12689592]
Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11029058]
EXISTENCE:direct assay:GO:0006334 nucleosome assembly [PMID:29339748]
EXISTENCE:direct assay:GO:0031151 histone H3K79 methyltransferase activity [PMID:12080090|PMID:18158898]
EXISTENCE:direct assay:GO:0034729 histone H3-K79 methylation [PMID:12080090]
EXISTENCE:genetic interaction:GO:0000077 DNA damage checkpoint signaling [PMID:17267293]
EXISTENCE:genetic interaction:GO:0000725 recombinational repair [PMID:17267293]
EXISTENCE:genetic interaction:GO:0006289 nucleotide-excision repair [PMID:17267293]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:17267293]
EXISTENCE:genetic interaction:GO:0051598 meiotic recombination checkpoint signaling [PMID:11029058]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:17267293]
EXISTENCE:mutant phenotype:GO:0000725 recombinational repair [PMID:17267293]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:17267293]
EXISTENCE:mutant phenotype:GO:0031151 histone H3K79 methyltransferase activity [PMID:12080090]
EXISTENCE:mutant phenotype:GO:0031452 negative regulation of heterochromatin formation [PMID:26587833]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11029058|PMID:26587833]
EXISTENCE:mutant phenotype:GO:0031571 mitotic G1 DNA damage checkpoint signaling [PMID:16166626]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:16166626]
EXISTENCE:mutant phenotype:GO:0034729 histone H3-K79 methylation [PMID:12080090]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:21460225]
Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response; single DOT1 gene produces two Dot1p isoforms from alternative translation start sites as a result of leaky scanning by the ribosome
Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication
MED13; NUT8; RYE3; SCA1; SRB9; SSX5; UME2
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:15601835]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:11383511|PMID:15477388|PMID:12200444]
EXISTENCE:direct assay:GO:0030332 cyclin binding [PMID:29212878]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8524287]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:11139488]
EXISTENCE:mutant phenotype:GO:0000435 positive regulation of transcription from RNA polymerase II promoter by galactose [PMID:15601835]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15601835]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
Putative hydrolase acting on ester bonds
EXISTENCE:direct assay:GO:0000795 synaptonemal complex [PMID:23326245|PMID:24098146]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11678331]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:15598532|PMID:23326245]
EXISTENCE:mutant phenotype:GO:0070193 synaptonemal complex organization [PMID:23326245]
Meiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:3915776]
Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
chromatin-binding transcription regulator ADA2
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:18950642]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:19737915]
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:7972120]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:19737915]
EXISTENCE:mutant phenotype:GO:0001786 phosphatidylserine binding [PMID:18950642]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:18950642]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:19737915]
Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
snoRNA-binding rRNA-processing protein UTP6
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309|PMID:12150911]
EXISTENCE:direct assay:GO:0034388 Pwp2p-containing subcomplex of 90S preribosome [PMID:15231838|PMID:17515605]
Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
EXISTENCE:genetic interaction:GO:0006407 rRNA export from nucleus [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:14627813]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:10705372|PMID:12464633]
EXISTENCE:genetic interaction:GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:12464633]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:10705372]
EXISTENCE:mutant phenotype:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:12464633]
Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000298 endopolyphosphatase activity [PMID:11447286|PMID:31175919]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:11566881]
EXISTENCE:direct assay:GO:0004309 exopolyphosphatase activity [PMID:17009950]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:17009950]
EXISTENCE:direct assay:GO:0006797 polyphosphate metabolic process [PMID:11447286]
EXISTENCE:direct assay:GO:0006798 polyphosphate catabolic process [PMID:17009950]
EXISTENCE:direct assay:GO:0016787 hydrolase activity [PMID:31175919]
EXISTENCE:mutant phenotype:GO:0000298 endopolyphosphatase activity [PMID:11447286]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:11566881]
EXISTENCE:mutant phenotype:GO:0004309 exopolyphosphatase activity [PMID:16862600]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:16862600]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:11447286]
Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10681558]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:15163410]
EXISTENCE:direct assay:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:10681558]
EXISTENCE:direct assay:GO:0051920 peroxiredoxin activity [PMID:9888818|PMID:17210445]
EXISTENCE:mutant phenotype:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:9888818]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:10681558]
Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004385 guanylate kinase activity [PMID:1334480]
EXISTENCE:mutant phenotype:GO:0004385 guanylate kinase activity [PMID:1334480|PMID:11063676]
EXISTENCE:mutant phenotype:GO:0046711 GDP biosynthetic process [PMID:11063676|PMID:1334480]
Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20826459]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:22210050]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:22210050|PMID:20709757|PMID:9694857]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:22210050]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:10589731]
EXISTENCE:mutant phenotype:GO:0015385 sodium:proton antiporter activity [PMID:10998367|PMID:15635088]
EXISTENCE:mutant phenotype:GO:0015386 potassium:proton antiporter activity [PMID:15635088]
EXISTENCE:mutant phenotype:GO:0030004 cellular monovalent inorganic cation homeostasis [PMID:10589731]
EXISTENCE:mutant phenotype:GO:0035725 sodium ion transmembrane transport [PMID:10998367|PMID:15635088]
EXISTENCE:mutant phenotype:GO:0061763 multivesicular body-lysosome fusion [PMID:29212874]
EXISTENCE:mutant phenotype:GO:0071805 potassium ion transmembrane transport [PMID:15635088]
Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:19578373]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:19578373]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:10395901]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238398]
EXISTENCE:mutant phenotype:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238398]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238398]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11238398]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:23129771]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:10395901]
EXISTENCE:mutant phenotype:GO:0006997 nucleus organization [PMID:10395901]
E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
ARS439; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:16929305]
Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16647879]
EXISTENCE:direct assay:GO:0016409 palmitoyltransferase activity [PMID:16186255]
EXISTENCE:direct assay:GO:0018345 protein palmitoylation [PMID:16186255]
Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
EXISTENCE:direct assay:GO:0070985 transcription factor TFIIK complex [PMID:19818408]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:10681587]
Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:10022942]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:3285180]
EXISTENCE:mutant phenotype:GO:0000772 mating pheromone activity [PMID:3285180]
Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2
mitochondrial 54S ribosomal protein YmL28
Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:11212916]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11212916|PMID:19906648]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1588961|PMID:21127045]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21127045]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21127045]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:11212916]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:11212916|PMID:19906648]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:10648791]
Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0010629 negative regulation of gene expression [PMID:8005438]
hypothetical protein; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17448464|PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17448464|PMID:22842922|PMID:14562095]
EXISTENCE:direct assay:GO:0018216 peptidyl-arginine methylation [PMID:11856739]
EXISTENCE:direct assay:GO:0019702 protein arginine N5-methyltransferase activity [PMID:11856739]
EXISTENCE:mutant phenotype:GO:0018216 peptidyl-arginine methylation [PMID:9873020]
EXISTENCE:mutant phenotype:GO:0019702 protein arginine N5-methyltransferase activity [PMID:9873020]
Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:genetic interaction:GO:0000196 cell wall integrity MAPK cascade [PMID:10567559]
Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:14981247]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:9427746]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:15973437]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11739407]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:9427746]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:10397773]
Essential t-SNARE that mediates fusion of vesicles with the late Golgi; forms a complex with Tlg2p and Vti1p; mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:direct assay:GO:0051568 histone H3-K4 methylation [PMID:11742990|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11687631|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0051568 histone H3-K4 methylation [PMID:11752412|PMID:11805083]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:19237599|PMID:16407407]
EXISTENCE:mutant phenotype:GO:0008053 mitochondrial fusion [PMID:11257114|PMID:12808034]
Outer membrane component of the mitochondrial fusion machinery; binds to Fzo1p and Mgm1p to link these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but likely not a transporter; similar to human SLC25A46 implicated in optic atroprophy spectrum disorder and Leigh syndrome
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:23212245]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:23212245]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23212245]
Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22266655]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567|PMID:11081632]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at positions A2280 and A2281
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII)
ARS440; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0000387 spliceosomal snRNP assembly [PMID:9326489]
Splicing factor; component of the U4/U6-U5 snRNP complex
hypothetical protein; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
AMP-activated serine/threonine-protein kinase catalytic subunit SNF1
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:29643469]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11486005|PMID:2557546|PMID:27524664|PMID:7913470|PMI D:27592031]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:26198097]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17237508]
EXISTENCE:direct assay:GO:0005641 nuclear envelope lumen [PMID:17237508]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17237508]
EXISTENCE:direct assay:GO:0006995 cellular response to nitrogen starvation [PMID:12024013]
EXISTENCE:direct assay:GO:0031588 nucleotide-activated protein kinase complex [PMID:12393914|PMID:2481228]
EXISTENCE:genetic interaction:GO:0000132 establishment of mitotic spindle orientation [PMID:29643469]
EXISTENCE:genetic interaction:GO:0017148 negative regulation of translation [PMID:18955495]
EXISTENCE:genetic interaction:GO:2000222 positive regulation of pseudohyphal growth [PMID:12024013]
EXISTENCE:mutant phenotype:GO:0000132 establishment of mitotic spindle orientation [PMID:29643469]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:11095711|PMID:12556493]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:27524664]
EXISTENCE:mutant phenotype:GO:0004679 AMP-activated protein kinase activity [PMID:11486005|PMID:2557546|PMID:7913470]
EXISTENCE:mutant phenotype:GO:0007155 cell adhesion [PMID:12556493]
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:11486014]
EXISTENCE:mutant phenotype:GO:0017148 negative regulation of translation [PMID:18955495]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:32005698]
EXISTENCE:mutant phenotype:GO:0045722 positive regulation of gluconeogenesis [PMID:8628258|PMID:8710504|PMID:9111319]
EXISTENCE:mutant phenotype:GO:0071940 fungal-type cell wall assembly [PMID:24486034]
EXISTENCE:mutant phenotype:GO:0090606 single-species surface biofilm formation [PMID:12024013]
EXISTENCE:mutant phenotype:GO:1900436 positive regulation of filamentous growth of a population of unicellular organisms in response to starvation [PMID:22904036]
EXISTENCE:mutant phenotype:GO:2000222 positive regulation of pseudohyphal growth [PMID:12024013|PMID:16980405]
EXISTENCE:physical interaction:GO:0031588 nucleotide-activated protein kinase complex [PMID:2481228|PMID:9121458]
AMP-activated S/T protein kinase; complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-repressed gene transcription, heat shock, sporulation, and peroxisome biogenesis; active form involved in mitotic spindle alignment in non-limiting glucose; regulates filamentous growth and acts as a non-canonical GEF-activating Arf3p during invasive growth; sumoylation targets it for Ub-ligase mediated destruction; contains polyhistidine tract that acts as pH sensing module
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:17881380]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:7958920]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:10523674]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:22977255]
EXISTENCE:mutant phenotype:GO:0030541 plasmid partitioning [PMID:10523674]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:11880623|PMID:7958920|PMID:17881380]
Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27129769]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:12707309]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:27129769|PMID:26928762]
EXISTENCE:direct assay:GO:0007031 peroxisome organization [PMID:12707309]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:12707309]
ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:10825185]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9094309]
EXISTENCE:direct assay:GO:1990526 Ste12p-Dig1p-Dig2p complex [PMID:16782869]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9094309|PMID:9860980]
EXISTENCE:genetic interaction:GO:2000218 negative regulation of invasive growth in response to glucose limitation [PMID:8918885|PMID:9094309]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:8918885|PMID:9094309]
MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:2676517|PMID:26928762]
EXISTENCE:direct assay:GO:0004035 alkaline phosphatase activity [PMID:8499492]
EXISTENCE:mutant phenotype:GO:0046496 nicotinamide nucleotide metabolic process [PMID:21349851]
Repressible vacuolar alkaline phosphatase; controls polyphosphate content; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
U2-type spliceosomal complex subunit CWC21
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:19789211]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:19789211]
Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
EXISTENCE:direct assay:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:1550992]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:7490293]
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:1541391]
EXISTENCE:mutant phenotype:GO:0006491 N-glycan processing [PMID:1495972]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:1495972]
Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0016239 positive regulation of macroautophagy [PMID:12446664]
EXISTENCE:mutant phenotype:GO:0030029 actin filament-based process [PMID:9153752]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:12377769]
Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:16299513|PMID:14690608]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:14690608|PMID:23580526]
EXISTENCE:physical interaction:GO:0042393 histone binding [PMID:16299513]
Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:14562095]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:18032584]
Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
3,4-dihydroxy-2-butanone-4-phosphate synthase RIB3
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:12595523]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12595523]
EXISTENCE:mutant phenotype:GO:0008686 3,4-dihydroxy-2-butanone-4-phosphate synthase activity [PMID:7559556]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:12595523]
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:5366000]
3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase); required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
EXISTENCE:direct assay:GO:0005881 cytoplasmic microtubule [PMID:21633107|PMID:15642746]
EXISTENCE:genetic interaction:GO:0030473 nuclear migration along microtubule [PMID:9201714]
EXISTENCE:mutant phenotype:GO:0008574 plus-end-directed microtubule motor activity [PMID:15642746]
EXISTENCE:mutant phenotype:GO:0030473 nuclear migration along microtubule [PMID:9201714|PMID:15642746]
EXISTENCE:mutant phenotype:GO:0040001 establishment of mitotic spindle localization [PMID:21633107|PMID:23577064]
EXISTENCE:physical interaction:GO:0005868 cytoplasmic dynein complex [PMID:9658168]
Dynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8
EXISTENCE:physical interaction:GO:0000811 GINS complex [PMID:12730134|PMID:12768207]
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:22918958|PMID:10074427]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12221112]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:12221112]
EXISTENCE:direct assay:GO:0018107 peptidyl-threonine phosphorylation [PMID:10074427]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:18034155]
EXISTENCE:genetic interaction:GO:0010606 positive regulation of cytoplasmic mRNA processing body assembly [PMID:21163942]
EXISTENCE:genetic interaction:GO:0018105 peptidyl-serine phosphorylation [PMID:25767889]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:22918958]
EXISTENCE:genetic interaction:GO:0060211 regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:21163942]
EXISTENCE:mutant phenotype:GO:0000196 cell wall integrity MAPK cascade [PMID:10567559]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11807089]
EXISTENCE:mutant phenotype:GO:0018107 peptidyl-threonine phosphorylation [PMID:10074427]
EXISTENCE:mutant phenotype:GO:0071902 positive regulation of protein serine/threonine kinase activity [PMID:12221112]
Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ypk2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0006882 cellular zinc ion homeostasis [PMID:15060275]
Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:20404342]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:21807901]
EXISTENCE:mutant phenotype:GO:0044183 protein folding chaperone [PMID:22927643]
EXISTENCE:physical interaction:GO:0044183 protein folding chaperone [PMID:22927643]
Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog
mitochondrial 37S ribosomal protein RSM28
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:15701796]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:15701796]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:15701796]
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:17194786]
Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation
EXISTENCE:direct assay:GO:0005768 endosome [PMID:23417307]
EXISTENCE:direct assay:GO:0033263 CORVET complex [PMID:17488625]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:2202738]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:2202738]
Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
EXISTENCE:direct assay:GO:0000900 mRNA regulatory element binding translation repressor activity [PMID:15198983]
EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR binding [PMID:15198983]
EXISTENCE:direct assay:GO:0015934 large ribosomal subunit [PMID:19806183]
EXISTENCE:direct assay:GO:0017148 negative regulation of translation [PMID:15198983]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:27458021]
EXISTENCE:direct assay:GO:0048027 mRNA 5'-UTR binding [PMID:31323064]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:19806183|PMID:27458021|PMID:31779129]
EXISTENCE:mutant phenotype:GO:0050821 protein stabilization [PMID:31779129]
EXISTENCE:mutant phenotype:GO:0101031 chaperone complex [PMID:31779129]
Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16233524|PMID:22842922]
EXISTENCE:mutant phenotype:GO:0005365 myo-inositol transmembrane transporter activity [PMID:2040626]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:24603354]
EXISTENCE:mutant phenotype:GO:0015798 myo-inositol transport [PMID:2040626]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:2040626]
Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0048279 vesicle fusion with endoplasmic reticulum [PMID:8612273]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:19346454]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:8334998|PMID:11914276|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1537327]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:12853481|PMID:12893879|PMID:9214619]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:8612273]
Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER and for efficient nuclear fusion during mating; mediates Sey1p-independent homotypic ER fusion; interacts with the Dsl1p complex through Tip20p
EXISTENCE:direct assay:GO:0000228 nuclear chromosome [PMID:11983176]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:11983176]
EXISTENCE:direct assay:GO:0045184 establishment of protein localization [PMID:11983176]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:11060031]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:12181334]
EXISTENCE:mutant phenotype:GO:0045184 establishment of protein localization [PMID:11983176]
Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p and contributes to its activation; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:23268442]
EXISTENCE:genetic interaction:GO:0000448 cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:23268442]
Ribosomal 60S subunit protein L37B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12464632]
EXISTENCE:genetic interaction:GO:0006974 cellular response to DNA damage stimulus [PMID:12760057]
Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004478 methionine adenosyltransferase activity [PMID:18078345]
EXISTENCE:mutant phenotype:GO:0006555 methionine metabolic process [PMID:4585174]
EXISTENCE:mutant phenotype:GO:0006556 S-adenosylmethionine biosynthetic process [PMID:17426150]
S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon
ARS442; Putative replication origin; identified in multiple array studies, not yet confirmed by plasmid-based assay
EXISTENCE:direct assay:GO:0008195 phosphatidate phosphatase activity [PMID:10685032]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:10685032]
Lipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
Asn and gln rich hypothetical protein; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; capable of forming the prion [PSP1+]; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0051321 meiotic cell cycle [PMID:22194413]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:22194413]
EXISTENCE:mutant phenotype:GO:0070193 synaptonemal complex organization [PMID:22194413]
Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
ARS443; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:9813093]
EXISTENCE:genetic interaction:GO:0007117 budding cell bud growth [PMID:15107529]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:9813093]
EXISTENCE:mutant phenotype:GO:0007117 budding cell bud growth [PMID:9925642]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:9925642]
EXISTENCE:mutant phenotype:GO:1901900 regulation of protein localization to cell division site [PMID:28148650]
Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; negatively regulates Fpk1p kinase that stimulates phospholipid flippases Dnf1p and Dnf2p; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0015193 L-proline transmembrane transporter activity [PMID:14968425]
EXISTENCE:genetic interaction:GO:0098718 serine import across plasma membrane [PMID:32845888]
EXISTENCE:mutant phenotype:GO:0090156 cellular sphingolipid homeostasis [PMID:32845888]
EXISTENCE:mutant phenotype:GO:0098718 serine import across plasma membrane [PMID:32845888]
Broad specificity amino acid permease; high-affinity glutamine permease; major serine permease with minor contributions from paralog Agp1p; role in serine uptake impacts cellular sphingolipid homeostasis; also transports Leu, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10364461|PMID:10579719]
EXISTENCE:direct assay:GO:0005940 septin ring [PMID:10364461|PMID:10579719]
EXISTENCE:direct assay:GO:0031386 protein tag [PMID:10364461|PMID:10579719]
Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:10103055]
EXISTENCE:mutant phenotype:GO:0006111 regulation of gluconeogenesis [PMID:8878673]
EXISTENCE:mutant phenotype:GO:0015976 carbon utilization [PMID:8878673]
Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:19703468]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:12586695]
Non-essential hypothetical protein; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs; deletion affects mitochondrial morphology
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:12684511]
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:12684511|PMID:18992757]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11958675|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11958675]
EXISTENCE:mutant phenotype:GO:0015036 disulfide oxidoreductase activity [PMID:9571241]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:9571241]
Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:21777356|PMID:14562095]
hypothetical protein that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10564276]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:10564276]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:10564276]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:8628314]
RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0004396 hexokinase activity [PMID:34354536]
EXISTENCE:direct assay:GO:0019318 hexose metabolic process [PMID:34354536]
Hexokinase expressed during growth with low glucose levels; induced on non-fermentable carbon sources; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; protein abundance increases in response to DNA replication stress; EMI2 has a paralog, GLK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:17261844]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10417390|PMID:11914276]
EXISTENCE:direct assay:GO:0031090 organelle membrane [PMID:22144692]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:17261844]
EXISTENCE:mutant phenotype:GO:0005801 cis-Golgi network [PMID:17261844]
Acetylated cis-Golgi protein, homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; required for unconventional protein secretion (UPS) of Acb1p in response to starvation; forms amyloid fibrils under heat shock; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0003756 protein disulfide isomerase activity [PMID:16002399]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276|PMID:26928762]
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:16002399]
EXISTENCE:direct assay:GO:0019153 protein-disulfide reductase (glutathione) activity [PMID:16002399]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16002399]
EXISTENCE:genetic interaction:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:11157982]
EXISTENCE:genetic interaction:GO:0015035 protein-disulfide reductase activity [PMID:11157982]
EXISTENCE:mutant phenotype:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16002399]
Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
peptidylprolyl isomerase family protein FPR2
EXISTENCE:direct assay:GO:0003755 peptidyl-prolyl cis-trans isomerase activity [PMID:1380159]
EXISTENCE:direct assay:GO:0005528 FK506 binding [PMID:1380159]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1380159]
Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2
Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component
putative serine/threonine protein kinase SPS1
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:27182947]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:25409301|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25409301|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:3023934]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15755916]
EXISTENCE:mutant phenotype:GO:0051229 meiotic spindle disassembly [PMID:32788308]
EXISTENCE:mutant phenotype:GO:1903024 positive regulation of ascospore-type prospore membrane formation [PMID:27182947|PMID:32788308]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:32788308]
STE20-family GCKIII protein kinase; required for prospore membrane closure, efficient spore packaging, and localization of enzymes involved in spore wall synthesis; component of a non-canonical Hippo pathway with Cdc15p involved in spindle disassembly and sustained release of Cdc14p during anaphase II; forms a complex with Cdc15p; phosphorylation requires Cdc15p; required for Ssp1p phosphorylation and turnover; interacts with 14-3-3 proteins; expressed in late meiosis; nuclear and cytoplasmic
ARS446; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:12627398]
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:21135091]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:21135091]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:21135091]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9677411]
EXISTENCE:genetic interaction:GO:0006891 intra-Golgi vesicle-mediated transport [PMID:9677411]
ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
hypothetical protein; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:20406419|PMID:26928762]
hypothetical protein; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:15282305]
Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15923376|PMID:15306656]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2266; overexpression confers resistance to baking-associated stress
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:11532139]
Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
ubiquinol--cytochrome-c reductase subunit 7
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857]
EXISTENCE:mutant phenotype:GO:0006122 mitochondrial electron transport, ubiquinol to cytochrome c [PMID:2540976]
EXISTENCE:mutant phenotype:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:2540976]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:2540976]
EXISTENCE:mutant phenotype:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:11556808]
Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
bifunctional AP-4-A phosphorylase/ADP sulfurylase
EXISTENCE:direct assay:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863]
EXISTENCE:direct assay:GO:0009164 nucleoside catabolic process [PMID:2174863]
EXISTENCE:genetic interaction:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863]
EXISTENCE:genetic interaction:GO:0009164 nucleoside catabolic process [PMID:2174863]
EXISTENCE:mutant phenotype:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863]
EXISTENCE:mutant phenotype:GO:0009164 nucleoside catabolic process [PMID:2174863]
Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14690591]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14690591]
EXISTENCE:mutant phenotype:GO:0004594 pantothenate kinase activity [PMID:19266201]
EXISTENCE:mutant phenotype:GO:0008204 ergosterol metabolic process [PMID:31409644]
EXISTENCE:mutant phenotype:GO:2001023 regulation of response to drug [PMID:31409644]
Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:14565975]
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19893618]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:14562095]
Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation
glutathione-independent methylglyoxalase
EXISTENCE:direct assay:GO:0000932 P-body [PMID:24706893]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:24706893|PMID:26777405]
EXISTENCE:direct assay:GO:0019172 glyoxalase III activity [PMID:24302734]
EXISTENCE:direct assay:GO:0019243 methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione [PMID:24302734]
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:26306045]
EXISTENCE:direct assay:GO:0061077 chaperone-mediated protein folding [PMID:26306045]
EXISTENCE:mutant phenotype:GO:0031669 cellular response to nutrient levels [PMID:24706893]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17395014]
Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0015891 siderophore transport [PMID:12196168]
Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall
glucose-inactivated glycerol proton symporter STL1
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15703210]
EXISTENCE:direct assay:GO:0015295 solute:proton symporter activity [PMID:15703210|PMID:17434487]
EXISTENCE:mutant phenotype:GO:0015295 solute:proton symporter activity [PMID:15703210|PMID:17434487]
EXISTENCE:mutant phenotype:GO:0015793 glycerol transmembrane transport [PMID:17434487|PMID:15703210]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15703210]
Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:17054397|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0016831 carboxy-lyase activity [PMID:11693915|PMID:8181743]
EXISTENCE:mutant phenotype:GO:0019439 aromatic compound catabolic process [PMID:11693915]
EXISTENCE:mutant phenotype:GO:0046281 cinnamic acid catabolic process [PMID:20471595|PMID:8181743]
Phenylacrylic acid decarboxylase; decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; confers resistance to cinnamic acid; overexpression of both Pad1p and Fdc1p increases cinnamic acid decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; contains mRNA binding activity; homolog of E. coli UbiX
putative phenylacrylic acid decarboxylase FDC1
EXISTENCE:direct assay:GO:0016831 carboxy-lyase activity [PMID:25862228|PMID:25647642]
EXISTENCE:direct assay:GO:0033494 ferulate metabolic process [PMID:25862228]
EXISTENCE:mutant phenotype:GO:0016831 carboxy-lyase activity [PMID:25862228]
EXISTENCE:mutant phenotype:GO:0033494 ferulate metabolic process [PMID:25862228]
EXISTENCE:mutant phenotype:GO:0046281 cinnamic acid catabolic process [PMID:20471595]
Ferulic acid decarboxylase; involved in the decarboxylation of aromatic carboxylic acids such as phenylacrylic (cinnamic) acid, ferulic acid and coumaric acid and formation of the corresponding vinyl derivatives; overexpression of both Pad1p and Fdc1p increases decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm in an HTP study
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:18085829]
Protein similar to bacterial secreted DfsB proteins; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0033721 aldehyde dehydrogenase (NADP+) activity [PMID:25656103]
EXISTENCE:mutant phenotype:GO:1901426 response to furfural [PMID:25656103]
Aldehyde reductase; substrates include both aromatic and aliphatic aldehydes; uses NADPH as cofactor; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
hypothetical protein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to the vacuole; member of the seripauperin multigene family encoded mainly in subtelomeric regions
TEL04R; Telomeric region on the right arm of Chromosome IV; composed of an X element core sequence, X element combinatorial repeats, a short stretch of telomeric repeats, and a long Y' element
Y' element ATP-dependent helicase protein 1 copy 1
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:9837911]
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:9837911]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:9837911]
Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p