Saccharomyces cerevisiae S288C chromosome III, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The complete DNA sequence of yeast chromosome III
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (12-APR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (27-MAY-2010) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (21-MAY-2010) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006937.\n\nOn Apr 26, 2011 this sequence version replaced NC_001135.4.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL03L; Telomeric region on the left arm of Chromosome III; composed of an X element core sequence, X element combinatorial repeats, and a terminal stretch of telomeric repeats
ARS300; Autonomously Replicating Sequence
YCLWTy5-1; Ty5 element, LTR retrotransposon of the Copia (Pseudoviridae) group; this is the only near-full length Ty5 retrotransposon in S288C and it is non-functional
Pseudogene: encodes fragment of Ty Pol protein
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:21490148]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21490148]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0015299 solute:proton antiporter activity [PMID:21490148]
EXISTENCE:mutant phenotype:GO:0034775 glutathione transmembrane transport [PMID:21490148]
Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; imports glutathione from the vacuole and exports it through the plasma membrane; has a role in resistance to oxidative stress and modulation of the PKA pathway; GEX1 has a paralog, GEX2, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15572352]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015802 basic amino acid transport [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15572352]
Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication
HML; Mating type cassette - left; unexpressed complete copy of the mating-type genes, silenced by the Sir2 histone deacetylase and its associated SIR1, SIR3 and SIR4 proteins; mating-type switching involves replacement of the Ya or Yalpha sequences present at the MAT locus with either the a or alpha genes located at one of the HM loci in a process stimulated by the HO endonuclease; sequence replacement occurs by gene conversion, leaving the donor unaltered
ARS301; Inactive replication origin linked to the silent mating type locus HML; functions as a transcriptional silencer
Silenced copy of ALPHA2 at HML; homeobox-domain protein that associates with Mcm1p in haploid cells to repress a-specific gene expression and interacts with a1p in diploid cells to repress haploid-specific gene expression
transcriptional co-activator mating type protein alpha
Silenced copy of ALPHA1 at HML; ALPHA1 encodes a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression
ARS302; Inactive replication origin linked to the silent mating type locus HML; functions as a transcriptional silencer
ARS303; Autonomously Replicating Sequence on Chromosome III; inactive as replication origin at native locus near HML
ARS320; Autonomously Replicating Sequence on Chromosome III; inactive as replication origin at native locus near HML
EXISTENCE:direct assay:GO:0003941 L-serine ammonia-lyase activity [PMID:7042346]
EXISTENCE:direct assay:GO:0004794 L-threonine ammonia-lyase activity [PMID:7042346]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:genetic interaction:GO:0006565 L-serine catabolic process [PMID:7042346]
EXISTENCE:genetic interaction:GO:0006567 threonine catabolic process [PMID:7042346]
Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12594460]
EXISTENCE:genetic interaction:GO:0000011 vacuole inheritance [PMID:12594460]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:12594460]
EXISTENCE:physical interaction:GO:0000011 vacuole inheritance [PMID:12594460]
EXISTENCE:physical interaction:GO:0043495 protein-membrane adaptor activity [PMID:12642614]
EXISTENCE:physical interaction:GO:0071563 Myo2p-Vac17p-Vac8p transport complex [PMID:12594460]
Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p; involved in regulation of asymmetric inheritance of aggregated/misfolded proteins and age reset
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:direct assay:GO:0043596 nuclear replication fork [PMID:16103218]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:17483413]
EXISTENCE:genetic interaction:GO:0048478 replication fork protection [PMID:18851837]
EXISTENCE:mutant phenotype:GO:0000076 DNA replication checkpoint signaling [PMID:11331602|PMID:11715016|PMID:12944972]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:17323081]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:16137625]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:16219777]
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:17483413]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11331602]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11331602]
EXISTENCE:mutant phenotype:GO:0031573 mitotic intra-S DNA damage checkpoint signaling [PMID:11715016]
EXISTENCE:mutant phenotype:GO:0033262 regulation of nuclear cell cycle DNA replication [PMID:23178807]
EXISTENCE:mutant phenotype:GO:0043570 maintenance of DNA repeat elements [PMID:18321795]
EXISTENCE:mutant phenotype:GO:0048478 replication fork protection [PMID:18632578]
EXISTENCE:physical interaction:GO:0048478 replication fork protection [PMID:18851837]
S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; exposure to ethanol affects localization; mammalian claspin homolog
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11027267|PMID:12150911]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:15167894]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:11027267]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:11996121]
EXISTENCE:mutant phenotype:GO:0042274 ribosomal small subunit biogenesis [PMID:12150911]
Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:26649943]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:20195295]
EXISTENCE:mutant phenotype:GO:0006873 cellular ion homeostasis [PMID:10234789]
De novo-originated gene from non-coding sequence; protein can suppress mating and promote vegetative growth; shortens lag phase by physically interacting with Snf1p; binds with Matalpha2p to promoters of haploid-specific genes; functions in mating and fermentation, mediates crosstalk between reproduction and vegetative growth; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis
EXISTENCE:direct assay:GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding [PMID:20195295]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20195295]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:20195295]
Transcriptional repressor encoded by the FYV5 antisense strand; negatively regulates transcription of FYV5 by binding to the promoter on the sense strand
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061024 membrane organization [PMID:25955211]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:21944719|PMID:21987634|PMID:22009199]
Conserved component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic12p and Mic27p whose assembly and stability requires cardiolipin; homo-oligomers cause membrane bending; ortholog of human MINOS1
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:8218194]
EXISTENCE:direct assay:GO:0004222 metalloendopeptidase activity [PMID:8218194|PMID:8307027]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8307027|PMID:14562095|PMID:24390141]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:8307027]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:8218194]
EXISTENCE:direct assay:GO:0006508 proteolysis [PMID:8307027]
EXISTENCE:mutant phenotype:GO:0004222 metalloendopeptidase activity [PMID:8307027]
EXISTENCE:mutant phenotype:GO:0006508 proteolysis [PMID:8307027]
Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:21441307]
EXISTENCE:genetic interaction:GO:0007031 peroxisome organization [PMID:21441307]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:21441307]
Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:17101777]
EXISTENCE:genetic interaction:GO:0003700 DNA-binding transcription factor activity [PMID:17101777]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:8754797]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:17101777]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:17101777]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:8754797]
Transcription factor required for response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone; null mutant exhibits abnormal lipid metabolic phenotype
RE (recombination enhancer); Orientation-independent recombination enhancer; cis-acting region that controls mating-type dependent recombination along entire left arm of Chromosome III; Sir2p and the condensin complex are recruited to the RE exclusively in MATa cells, where the RE drives donor preference directional switching; RDT1 expression is MATa-specific
ARS304; Autonomously Replicating Sequence on Chromosome III
Small gene in chromosome III recombination enhancer; activated during mating-type switching; bound by condensin complex, deletion drastically changes chromosome III structure, alters donor preference; promoter functions as locus control region (LCR) that regulates both transcription and long-range chromatin interactions
27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10556316]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10556316|PMID:10648622]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614]
EXISTENCE:direct assay:GO:0031167 rRNA methylation [PMID:15546625]
EXISTENCE:genetic interaction:GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity [PMID:14636587]
EXISTENCE:genetic interaction:GO:0031167 rRNA methylation [PMID:14636587|PMID:15546625]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10556316|PMID:10648622]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10556316|PMID:10648622]
EXISTENCE:mutant phenotype:GO:0016435 rRNA (guanine) methyltransferase activity [PMID:15546625]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:15546625]
AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
EXISTENCE:direct assay:GO:0000030 mannosyltransferase activity [PMID:15635094]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9649520|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:15635094]
EXISTENCE:mutant phenotype:GO:0016485 protein processing [PMID:9649520]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16418276]
EXISTENCE:physical interaction:GO:1990529 glycosylphosphatidylinositol-mannosyltransferase I complex [PMID:15635094]
Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X
EXISTENCE:direct assay:GO:0004860 protein kinase inhibitor activity [PMID:23954433]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:23954433]
EXISTENCE:direct assay:GO:0006469 negative regulation of protein kinase activity [PMID:23954433]
EXISTENCE:genetic interaction:GO:0031505 fungal-type cell wall organization [PMID:11532139]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:9090049]
Protein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication
bifunctional AP-4-A phosphorylase/ADP sulfurylase
EXISTENCE:direct assay:GO:0004780 sulfate adenylyltransferase (ADP) activity [PMID:2556364]
EXISTENCE:direct assay:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863|PMID:2556364]
EXISTENCE:direct assay:GO:0009164 nucleoside catabolic process [PMID:2174863]
EXISTENCE:direct assay:GO:0009165 nucleotide biosynthetic process [PMID:2172926]
EXISTENCE:genetic interaction:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863]
EXISTENCE:genetic interaction:GO:0009164 nucleoside catabolic process [PMID:2174863]
EXISTENCE:mutant phenotype:GO:0004780 sulfate adenylyltransferase (ADP) activity [PMID:2556364]
EXISTENCE:mutant phenotype:GO:0008796 bis(5'-nucleosyl)-tetraphosphatase activity [PMID:2174863|PMID:2556364]
EXISTENCE:mutant phenotype:GO:0009164 nucleoside catabolic process [PMID:2174863]
AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication
ARS305; Efficient early-firing replication origin on Chromosome III
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11935221]
hypothetical protein; localizes to membrane fraction; YCL049C is not an essential gene
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCL048W-A has a paralog, YDR524C-B, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:15590821]
hypothetical protein; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
nicotinamide-nucleotide adenylyltransferase
EXISTENCE:direct assay:GO:0000309 nicotinamide-nucleotide adenylyltransferase activity [PMID:24759102]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:24759102]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24759102]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:22204397]
EXISTENCE:genetic interaction:GO:0000309 nicotinamide-nucleotide adenylyltransferase activity [PMID:24759102]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:21460040]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:21460040]
EXISTENCE:genetic interaction:GO:0034356 NAD biosynthesis via nicotinamide riboside salvage pathway [PMID:24759102]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:22204397]
EXISTENCE:physical interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:22204397]
Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0072546 EMC complex [PMID:19325107]
EXISTENCE:genetic interaction:GO:0034975 protein folding in endoplasmic reticulum [PMID:19325107]
Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0031942 i-AAA complex [PMID:18843051|PMID:16267274]
EXISTENCE:direct assay:GO:0051787 misfolded protein binding [PMID:18843051]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:18843051]
Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
EXISTENCE:direct assay:GO:0003756 protein disulfide isomerase activity [PMID:16002399|PMID:16413482]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:1761554]
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:16002399]
EXISTENCE:direct assay:GO:0036508 protein alpha-1,2-demannosylation [PMID:21700223]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16002399]
EXISTENCE:direct assay:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:21700223|PMID:27053108]
EXISTENCE:genetic interaction:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:21700223]
EXISTENCE:mutant phenotype:GO:0003756 protein disulfide isomerase activity [PMID:11157982]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:11157982]
EXISTENCE:mutant phenotype:GO:1904382 mannose trimming involved in glycoprotein ERAD pathway [PMID:21700223]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16002399]
Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
hypothetical protein; epitope-tagged protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0004340 glucokinase activity [PMID:8501032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:14562095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0006013 mannose metabolic process [PMID:237183]
EXISTENCE:direct assay:GO:0006096 glycolytic process [PMID:8501032]
EXISTENCE:genetic interaction:GO:0046323 glucose import [PMID:6300872]
EXISTENCE:mutant phenotype:GO:0004340 glucokinase activity [PMID:6341351]
EXISTENCE:mutant phenotype:GO:0006006 glucose metabolic process [PMID:6300872]
Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
glucose-induced degradation complex subunit GID7
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:18508925]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; localizes to peroxisomes in oleate-growing cells; computational analysis suggests that Gid7p and Moh1p have similar functions
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:17021250]
EXISTENCE:mutant phenotype:GO:0032974 amino acid transmembrane export from vacuole [PMID:17021250]
Vacuolar membrane protein; required for efflux of amino acids during autophagic body breakdown in vacuole; targeted to vacuole via AP-3 pathway; null mutation causes gradual loss of viability during starvation
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:10564276]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:10564276]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640|PMID:26777405]
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:10564276]
Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
hypothetical protein; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004364 glutathione transferase activity [PMID:12684511]
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:18992757|PMID:11875065|PMID:12684511]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:genetic interaction:GO:0010731 protein glutathionylation [PMID:20074363]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:9571241]
Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18298676]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:15651983|PMID:18298676|PMID:12388763]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:15651983]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:12388763]
EXISTENCE:genetic interaction:GO:0030036 actin cytoskeleton organization [PMID:12388763]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:12388763]
EXISTENCE:physical interaction:GO:0007015 actin filament organization [PMID:10512884]
Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat
peptide-methionine (R)-S-oxide reductase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:20799725]
EXISTENCE:direct assay:GO:0033743 peptide-methionine (R)-S-oxide reductase activity [PMID:15141092]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:11929995]
EXISTENCE:mutant phenotype:GO:0033743 peptide-methionine (R)-S-oxide reductase activity [PMID:15141092]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11929995]
Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9742096]
EXISTENCE:direct assay:GO:0019887 protein kinase regulator activity [PMID:10397774]
EXISTENCE:direct assay:GO:0032093 SAM domain binding [PMID:16337230]
EXISTENCE:genetic interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:9742096]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:8793874]
EXISTENCE:mutant phenotype:GO:0001402 signal transduction involved in filamentous growth [PMID:9738877]
EXISTENCE:mutant phenotype:GO:0007232 osmosensory signaling pathway via Sho1 osmosensor [PMID:11370856]
EXISTENCE:physical interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:9742096]
Adaptor protein for various signaling pathways; involved in mating response, invasive/filamentous growth, osmotolerance; acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0019843 rRNA binding [PMID:24130456]
EXISTENCE:direct assay:GO:0032545 CURI complex [PMID:17452446]
EXISTENCE:direct assay:GO:0034456 UTP-C complex [PMID:17515605]
EXISTENCE:genetic interaction:GO:0000028 ribosomal small subunit assembly [PMID:9271380]
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:9271380]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:9271380]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9271380]
Essential protein involved in rRNA processing and ribosome biogenesis; forms a complex with Utp22p; protein abundance increases in response to DNA replication stress
trifunctional histidinol dehydrogenase/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase
EXISTENCE:direct assay:GO:0004399 histidinol dehydrogenase activity [PMID:379004]
EXISTENCE:direct assay:GO:0004635 phosphoribosyl-AMP cyclohydrolase activity [PMID:379004]
EXISTENCE:direct assay:GO:0004636 phosphoribosyl-ATP diphosphatase activity [PMID:379004]
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:5919326|PMID:14190241]
EXISTENCE:mutant phenotype:GO:0004399 histidinol dehydrogenase activity [PMID:5919326]
EXISTENCE:mutant phenotype:GO:0004635 phosphoribosyl-AMP cyclohydrolase activity [PMID:5919326]
EXISTENCE:mutant phenotype:GO:0004636 phosphoribosyl-ATP diphosphatase activity [PMID:5919326]
Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:11756471|PMID:35235370|PMID:11511347]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:2277073]
EXISTENCE:direct assay:GO:0005819 spindle [PMID:2277073]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11511347]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11511347]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:9398684]
EXISTENCE:direct assay:GO:0019894 kinesin binding [PMID:35235370]
EXISTENCE:direct assay:GO:0031115 negative regulation of microtubule polymerization [PMID:20392838]
EXISTENCE:direct assay:GO:0035371 microtubule plus-end [PMID:20392838]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:16380440]
EXISTENCE:genetic interaction:GO:0000022 mitotic spindle elongation [PMID:7559759]
EXISTENCE:genetic interaction:GO:0030543 2-micrometer plasmid partitioning [PMID:28004422]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:2277073]
EXISTENCE:mutant phenotype:GO:0000743 nuclear migration involved in conjugation with cellular fusion [PMID:16380440]
EXISTENCE:mutant phenotype:GO:0000819 sister chromatid segregation [PMID:35235370]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:33752231]
EXISTENCE:mutant phenotype:GO:0030543 2-micrometer plasmid partitioning [PMID:28004422]
Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; promotes plus-end directed movement of Dyn1p; ortholog of mammalian CLIP-170
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11511345]
[PIN(+)] prion, named for [PSI+] INducibility; an infectious protein conformation that is generally an ordered protein aggregate
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:2690078]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12374868|PMID:3302672|PMID:19053807]
EXISTENCE:genetic interaction:GO:0000755 cytogamy [PMID:3302672]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:3313002|PMID:3302672]
EXISTENCE:mutant phenotype:GO:0031385 regulation of termination of mating projection growth [PMID:14734532]
EXISTENCE:mutant phenotype:GO:0032065 maintenance of protein location in cell cortex [PMID:18474625]
Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
hypothetical protein; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
ARS306; Early-firing autonomously replicating sequence on Chromosome III
EXISTENCE:direct assay:GO:0016657 oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor [PMID:22687599]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:19904831]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:19904831]
EXISTENCE:mutant phenotype:GO:0045922 negative regulation of fatty acid metabolic process [PMID:8701605]
Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27226538]
EXISTENCE:direct assay:GO:0006865 amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0015171 amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10654085]
EXISTENCE:genetic interaction:GO:0015193 L-proline transmembrane transporter activity [PMID:14968425]
EXISTENCE:genetic interaction:GO:0098718 serine import across plasma membrane [PMID:32845888]
EXISTENCE:mutant phenotype:GO:0098718 serine import across plasma membrane [PMID:32845888]
Broad-specificity, low-affinity amino acid permease; minor serine permease with major contributions from paralog Gnp1p; involved in uptake of asparagine, glutamine, and other amino acids; expression induced by amino acids and regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p)
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15901837]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15282802|PMID:9925642]
EXISTENCE:direct assay:GO:0005940 septin ring [PMID:15901837]
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:15901837]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:9925642]
EXISTENCE:mutant phenotype:GO:0007117 budding cell bud growth [PMID:9925642]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:9925642]
Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Glutamine tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the vacuole
YCLWTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations
EXISTENCE:direct assay:GO:0003862 3-isopropylmalate dehydrogenase activity [PMID:3071718]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:4355481]
EXISTENCE:mutant phenotype:GO:0003862 3-isopropylmalate dehydrogenase activity [PMID:6297759]
EXISTENCE:mutant phenotype:GO:0006097 glyoxylate cycle [PMID:23642236]
EXISTENCE:mutant phenotype:GO:0009098 leucine biosynthetic process [PMID:6297759]
Beta-isopropylmalate dehydrogenase (IMDH); catalyzes the third step in the leucine biosynthesis pathway; can additionally catalyze the conversion of beta-ethylmalate into alpha-ketovalerate
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19720832]
EXISTENCE:direct assay:GO:0016226 iron-sulfur cluster assembly [PMID:15220327]
EXISTENCE:direct assay:GO:0031071 cysteine desulfurase activity [PMID:15220327|PMID:12065597]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:11110795]
EXISTENCE:mutant phenotype:GO:0006879 cellular iron ion homeostasis [PMID:10551871]
EXISTENCE:mutant phenotype:GO:0016226 iron-sulfur cluster assembly [PMID:12970193]
EXISTENCE:mutant phenotype:GO:0034227 tRNA thio-modification [PMID:14722066|PMID:15220327]
EXISTENCE:mutant phenotype:GO:0070903 mitochondrial tRNA thio-modification [PMID:14722066]
EXISTENCE:physical interaction:GO:1990221 L-cysteine desulfurase complex [PMID:24045011]
Cysteine desulfurase involved in iron-sulfur cluster biogenesis; required for thio-modification of mitochondrial and cytoplasmic tRNAs; involved in DNA damage response by providing iron-sulfur cluster cofactors for DNA repair enzymes; essential protein located predominantly in mitochondria
EXISTENCE:mutant phenotype:GO:0007064 mitotic sister chromatid cohesion [PMID:11389843]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:16525505]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:21347277]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance
EXISTENCE:direct assay:GO:0000142 cellular bud neck contractile ring [PMID:7730410]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:25002677]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12972503]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:25002677]
EXISTENCE:genetic interaction:GO:0043547 positive regulation of GTPase activity [PMID:25002677]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9744878]
EXISTENCE:mutant phenotype:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:25002677]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:2065354]
Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern in haploids; localizes with septins to the bud neck contractile ring in mitosis
hypothetical protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
single-stranded telomeric DNA-binding/mRNA-binding protein
EXISTENCE:direct assay:GO:0000723 telomere maintenance [PMID:12519786]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:14769921]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:12634846|PMID:14769921|PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12634846|PMID:22932476|PMID:26359986]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:33910495]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:26777405|PMID:33910495]
EXISTENCE:direct assay:GO:0043047 single-stranded telomeric DNA binding [PMID:7777547|PMID:7800479]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:12519786]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:12519786]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:12634846]
EXISTENCE:mutant phenotype:GO:0043047 single-stranded telomeric DNA binding [PMID:7777547]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:24452287]
Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:12052880]
EXISTENCE:direct assay:GO:0035064 methylated histone binding [PMID:21685874]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:22224423]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:21685874]
EXISTENCE:mutant phenotype:GO:0072742 SAGA complex localization to transcription regulatory region [PMID:21685874]
EXISTENCE:mutant phenotype:GO:0140671 ADA complex [PMID:21734642]
EXISTENCE:physical interaction:GO:0140671 ADA complex [PMID:21734642]
Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
acetolactate synthase regulatory subunit
EXISTENCE:direct assay:GO:0003984 acetolactate synthase activity [PMID:16390333|PMID:8972574]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005948 acetolactate synthase complex [PMID:10213630]
EXISTENCE:direct assay:GO:0009082 branched-chain amino acid biosynthetic process [PMID:8972574]
EXISTENCE:direct assay:GO:0030234 enzyme regulator activity [PMID:8972574]
EXISTENCE:mutant phenotype:GO:0003984 acetolactate synthase activity [PMID:16390333]
Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
ubiquitin-binding ESCRT-I subunit protein STP22
EXISTENCE:curator inference:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:15086794]
EXISTENCE:direct assay:GO:0000813 ESCRT I complex [PMID:15044434]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:11511343]
EXISTENCE:direct assay:GO:0009898 cytoplasmic side of plasma membrane [PMID:20028738]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:19380877]
EXISTENCE:direct assay:GO:0061709 reticulophagy [PMID:31802527]
EXISTENCE:mutant phenotype:GO:0006612 protein targeting to membrane [PMID:11511343]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:11511343]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:17145965]
EXISTENCE:mutant phenotype:GO:1902915 negative regulation of protein polyubiquitination [PMID:23645667]
EXISTENCE:mutant phenotype:GO:1904669 ATP export [PMID:26585826]
EXISTENCE:physical interaction:GO:0000813 ESCRT I complex [PMID:16615894]
Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
H(+)-transporting V0 sector ATPase subunit e
EXISTENCE:mutant phenotype:GO:0000032 cell wall mannoprotein biosynthetic process [PMID:14734024]
EXISTENCE:mutant phenotype:GO:0000220 vacuolar proton-transporting V-type ATPase, V0 domain [PMID:14970230]
EXISTENCE:mutant phenotype:GO:0000329 fungal-type vacuole membrane [PMID:14970230]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:14970230]
EXISTENCE:mutant phenotype:GO:0042625 ATPase-coupled ion transmembrane transporter activity [PMID:14970230]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:14970230]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:16569636]
EXISTENCE:physical interaction:GO:0065003 protein-containing complex assembly [PMID:16569636]
Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15923376]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:18332121|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15923376|PMID:18332121]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U966 and of 5.8S rRNA at position 73
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:24434579]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0034389 lipid droplet organization [PMID:26572621]
EXISTENCE:mutant phenotype:GO:0090155 negative regulation of sphingolipid biosynthetic process [PMID:31594806]
Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2
ARS307; Autonomously Replicating Sequence on Chromosome III
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:9799363]
EXISTENCE:mutant phenotype:GO:0008444 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity [PMID:9545322]
EXISTENCE:mutant phenotype:GO:0032049 cardiolipin biosynthetic process [PMID:9545322]
Phosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
hypothetical protein; YCL002C is not an essential gene
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:22761830]
EXISTENCE:direct assay:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11238450]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:direct assay:GO:0030137 COPI-coated vesicle [PMID:11238450]
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:8707815]
EXISTENCE:mutant phenotype:GO:0006890 retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum [PMID:11238450]
EXISTENCE:mutant phenotype:GO:0030137 COPI-coated vesicle [PMID:11238450]
Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
hypothetical protein; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
hypothetical protein; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
ARS308; Autonomously Replicating Sequence on Chromosome III
hypothetical protein; conserved among S. cerevisiae strains; YCR001W is not an essential gene
EXISTENCE:curator inference:GO:0030011 maintenance of cell polarity [PMID:10882120]
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:9884239|PMID:31990274]
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:18552279]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:14993234]
EXISTENCE:direct assay:GO:0005200 structural constituent of cytoskeleton [PMID:18550837|PMID:22144691|PMID:29321251]
EXISTENCE:direct assay:GO:0005545 1-phosphatidylinositol binding [PMID:12665577]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:18826657|PMID:24390141]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:18826657]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:18550837]
EXISTENCE:direct assay:GO:0032160 septin filament array [PMID:15282341]
EXISTENCE:direct assay:GO:0042764 ascospore-type prospore [PMID:18701287]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:direct assay:GO:0043934 sporulation [PMID:18701287]
EXISTENCE:direct assay:GO:0060090 molecular adaptor activity [PMID:15901837]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:12665577]
EXISTENCE:direct assay:GO:0072687 meiotic spindle [PMID:18826657]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:4950437]
EXISTENCE:mutant phenotype:GO:0000921 septin ring assembly [PMID:14993234]
EXISTENCE:mutant phenotype:GO:0010458 exit from mitosis [PMID:12699621]
EXISTENCE:physical interaction:GO:0031105 septin complex [PMID:9813094]
Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
mitochondrial 54S ribosomal protein YmL32
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:2060626]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2060626]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:2060626]
Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21442317|PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
hypothetical protein; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0004108 citrate (Si)-synthase activity [PMID:2181273]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25982115]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:16877773|PMID:25982115]
EXISTENCE:genetic interaction:GO:0004108 citrate (Si)-synthase activity [PMID:2181273]
EXISTENCE:genetic interaction:GO:0006101 citrate metabolic process [PMID:12913175]
Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YCR006C is not an essential gene
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; can mutate to suppress +1 frameshift mutations in proline codons
Putative integral membrane protein; member of DUP240 gene family; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCR007C is not an essential gene
Asparagine tRNA (tRNA-Asn), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25117470]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:25117470]
EXISTENCE:genetic interaction:GO:0000082 G1/S transition of mitotic cell cycle [PMID:12518319]
EXISTENCE:genetic interaction:GO:0008104 protein localization [PMID:21237705]
EXISTENCE:mutant phenotype:GO:0009249 protein lipoylation [PMID:25117470]
EXISTENCE:mutant phenotype:GO:0009594 detection of nutrient [PMID:32191698]
EXISTENCE:mutant phenotype:GO:0030003 cellular cation homeostasis [PMID:10207057]
EXISTENCE:mutant phenotype:GO:0045807 positive regulation of endocytosis [PMID:32191698]
EXISTENCE:mutant phenotype:GO:1903329 regulation of iron-sulfur cluster assembly [PMID:25117470]
Ser/Thr protein kinase involved in salt tolerance, nutrient response; functions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:9566960]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20610658|PMID:27093085]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:16239147]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:12477802]
EXISTENCE:direct assay:GO:0060988 lipid tube assembly [PMID:20610658]
EXISTENCE:direct assay:GO:0097320 plasma membrane tubulation [PMID:27093085]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:11096097]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:8590801]
EXISTENCE:mutant phenotype:GO:0006970 response to osmotic stress [PMID:1776363]
EXISTENCE:mutant phenotype:GO:0030036 actin cytoskeleton organization [PMID:7891662]
EXISTENCE:mutant phenotype:GO:0031532 actin cytoskeleton reorganization [PMID:7891662]
EXISTENCE:mutant phenotype:GO:0042594 response to starvation [PMID:1776363]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:8590801]
EXISTENCE:physical interaction:GO:0008092 cytoskeletal protein binding [PMID:10388809]
EXISTENCE:physical interaction:GO:1990528 Rvs161p-Rvs167p complex [PMID:20610658]
Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
ARS309; Autonomously Replicating Sequence on Chromosome III
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:17395151|PMID:16622836]
EXISTENCE:mutant phenotype:GO:0006846 acetate transport [PMID:14968426]
EXISTENCE:mutant phenotype:GO:0008519 ammonium transmembrane transporter activity [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0015123 acetate transmembrane transporter activity [PMID:14968426]
EXISTENCE:mutant phenotype:GO:0019740 nitrogen utilization [PMID:12429834]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:14968426]
EXISTENCE:mutant phenotype:GO:0072488 ammonium transmembrane transport [PMID:12429834]
Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11914276]
Putative ATP-dependent permease of the ABC transporter family
EXISTENCE:direct assay:GO:0004618 phosphoglycerate kinase activity [PMID:6254992]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:27385335|PMID:11914276]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:16823961|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:400791]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:400791]
3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:8265341]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:8265341]
EXISTENCE:direct assay:GO:0006302 double-strand break repair [PMID:12235149]
EXISTENCE:direct assay:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:10438542|PMID:15342630]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:10438542]
DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
EXISTENCE:direct assay:GO:0042131 thiamine phosphate phosphatase activity [PMID:26071590]
EXISTENCE:mutant phenotype:GO:0006817 phosphate ion transport [PMID:25725023]
Protein required for cold tolerance; involved in phosphate uptake; YCR015C is not an essential gene
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:9160748]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:9160748]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376|PMID:9472021]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U1042
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis;can mutate to suppress +1 frameshift mutations in glycine codons
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11427965]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11427965]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11427965]
EXISTENCE:mutant phenotype:GO:0006505 GPI anchor metabolic process [PMID:27462707]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:17714445|PMID:17761529]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:11427965]
GPI lipid remodelase; responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety; can also use lyso-GPI protein anchors and various base resistant lipids as substrates; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
EXISTENCE:genetic interaction:GO:0006364 rRNA processing [PMID:2179050]
Protein involved in the processing of pre-rRNA to mature rRNA; contains a C2/C2 zinc finger motif; srd1 mutation suppresses defects caused by the rrp1-1 mutation
EXISTENCE:curator inference:GO:0006413 translational initiation [PMID:9023104]
EXISTENCE:direct assay:GO:0016282 eukaryotic 43S preinitiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0071074 eukaryotic initiation factor eIF2 binding [PMID:14698289]
Methionine tRNA (tRNA-Met), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:8915539]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8915539]
EXISTENCE:mutant phenotype:GO:0030533 triplet codon-amino acid adaptor activity [PMID:17560369]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:17560369]
Lysine tRNA (tRNA-Lys), predicted by tRNAscan-SE analysis; a small portion is imported into mitochondria via interaction with mt lysyl-tRNA synthetase Msk1p and is necessary to decode AAG codons at high temperature, when base modification of mt-encoded tRNA-Lys is reduced
Protein necessary for stability of L-A dsRNA-containing particles
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:16850348]
EXISTENCE:expression pattern:GO:0006772 thiamine metabolic process [PMID:10383756]
hypothetical protein; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
EXISTENCE:direct assay:GO:0031417 NatC complex [PMID:10504710]
EXISTENCE:mutant phenotype:GO:0004596 peptide alpha-N-acetyltransferase activity [PMID:11274203]
EXISTENCE:mutant phenotype:GO:0006474 N-terminal protein amino acid acetylation [PMID:11274203]
Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
EXISTENCE:curator inference:GO:0003682 chromatin binding [PMID:12417720]
EXISTENCE:curator inference:GO:0006338 chromatin remodeling [PMID:12417720]
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:12417720]
Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:1535043|PMID:16622836]
EXISTENCE:mutant phenotype:GO:0032780 negative regulation of ATP-dependent activity [PMID:9250391]
Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
hypothetical protein; conserved among S. cerevisiae strains; YCR022C is not an essential gene
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:12562762]
Vacuolar membrane hypothetical protein; targeted to vacuole via AP-3 pathway; member of multidrug resistance family; not an essential gene
EXISTENCE:direct assay:GO:0004816 asparagine-tRNA ligase activity [PMID:9030748]
EXISTENCE:direct assay:GO:0006421 asparaginyl-tRNA aminoacylation [PMID:9030748]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:9030748]
EXISTENCE:mutant phenotype:GO:0070145 mitochondrial asparaginyl-tRNA aminoacylation [PMID:9030748]
Mitochondrial asparaginyl-tRNA synthetase
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:1532582]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:1532582]
EXISTENCE:genetic interaction:GO:0030234 enzyme regulator activity [PMID:8063750]
EXISTENCE:genetic interaction:GO:0032781 positive regulation of ATP-dependent activity [PMID:8063750]
Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
hypothetical protein; conserved across S. cerevisiae strains; YCR025C is not an essential gene
nucleotide diphosphatase/phosphodiesterase NPP1
EXISTENCE:direct assay:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
EXISTENCE:genetic interaction:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:genetic interaction:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0009141 nucleoside triphosphate metabolic process [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0016036 cellular response to phosphate starvation [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants
ARS310; Compound autonomously replicating sequence on Chromosome III
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:17101785]
Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:15706032]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15706032]
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:10373490]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:15020461]
EXISTENCE:mutant phenotype:GO:0015233 pantothenate transmembrane transporter activity [PMID:10373490]
EXISTENCE:mutant phenotype:GO:0015887 pantothenate transmembrane transport [PMID:10373490]
Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:1324172]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:1324172|PMID:24769239|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431]
EXISTENCE:direct assay:GO:0090297 positive regulation of mitochondrial DNA replication [PMID:26184436]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:1324172]
ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:26179915]
EXISTENCE:direct assay:GO:0001400 mating projection base [PMID:11014808]
EXISTENCE:direct assay:GO:0004857 enzyme inhibitor activity [PMID:19962315]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11014808]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11014808]
EXISTENCE:direct assay:GO:0043086 negative regulation of catalytic activity [PMID:19962315]
EXISTENCE:direct assay:GO:0061645 endocytic patch [PMID:19962315]
EXISTENCE:genetic interaction:GO:0032185 septin cytoskeleton organization [PMID:18791237]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:19776351]
EXISTENCE:mutant phenotype:GO:0032185 septin cytoskeleton organization [PMID:18791237]
EXISTENCE:physical interaction:GO:0032185 septin cytoskeleton organization [PMID:18791237]
Negative regulator of WASP-Arp23 complex; involved in endocytic site formation; directly inhibits Las17p stimulation of Arp23 complex-mediated actin assembly in vitro; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of large subunit (LSU) rRNA at position U2347
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:9858605]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:2277060|PMID:8293976]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15125836]
Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
EXISTENCE:mutant phenotype:GO:0000454 snoRNA guided rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
H/ACA box small nucleolar RNA (snoRNA); guides pseudouridylation of large subunit (LSU) rRNA at position U2735 and small subunit (SSU) rRNA at position U466
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:35343566]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15296494]
EXISTENCE:direct assay:GO:0031267 small GTPase binding [PMID:35343566]
EXISTENCE:mutant phenotype:GO:0006497 protein lipidation [PMID:35343566]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:15296494]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:35343566]
EXISTENCE:mutant phenotype:GO:0009268 response to pH [PMID:9509574]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15296494]
Endosomal protein involved in endocytosis and autophagy; homologous to Chediak-Higashi syndrome and Beige proteins, both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:19379692]
EXISTENCE:mutant phenotype:GO:0032874 positive regulation of stress-activated MAPK cascade [PMID:18487345]
Set3C histone deacetylase scaffold; can act as prion [ESI+] (Expressed Subtelomeric Information) that drives emergence and transgenerational inheritance of activated chromatin state; prion conformer is triggered by transient Snt1p phosphorylation upon cell cycle arrest; [ESI+] reshapes the activity Set3C, recruiting RNAPII, interfering with Rap1p binding to activate genes in otherwise repressed subtelomeric domains; relocalizes to cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12087109]
EXISTENCE:genetic interaction:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:23325927]
EXISTENCE:mutant phenotype:GO:0009922 fatty acid elongase activity [PMID:12684876]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:9832547]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9211877]
EXISTENCE:mutant phenotype:GO:0030497 fatty acid elongation [PMID:9211877]
EXISTENCE:mutant phenotype:GO:0042761 very long-chain fatty acid biosynthetic process [PMID:23325927]
Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
exosome non-catalytic core subunit RRP43
EXISTENCE:curator inference:GO:0043628 regulatory ncRNA 3'-end processing [PMID:10465791]
EXISTENCE:curator inference:GO:0070478 nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070481 nuclear-transcribed mRNA catabolic process, non-stop decay [PMID:10465791]
EXISTENCE:curator inference:GO:0070651 nonfunctional rRNA decay [PMID:10465791]
EXISTENCE:curator inference:GO:0071051 polyadenylation-dependent snoRNA 3'-end processing [PMID:10465791]
EXISTENCE:direct assay:GO:0000176 nuclear exosome (RNase complex) [PMID:10465791|PMID:19046973]
EXISTENCE:direct assay:GO:0000177 cytoplasmic exosome (RNase complex) [PMID:19046973|PMID:10465791]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:9891085]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:9891085]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9891085]
EXISTENCE:direct assay:GO:0071038 nuclear polyadenylation-dependent tRNA catabolic process [PMID:15828860|PMID:17643380]
EXISTENCE:mutant phenotype:GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:10508172|PMID:12364597|PMID:9390555]
EXISTENCE:mutant phenotype:GO:0006397 mRNA processing [PMID:27385342]
EXISTENCE:mutant phenotype:GO:0034427 nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' [PMID:12364597]
EXISTENCE:mutant phenotype:GO:0071035 nuclear polyadenylation-dependent rRNA catabolic process [PMID:18940861]
EXISTENCE:mutant phenotype:GO:0071042 nuclear polyadenylation-dependent mRNA catabolic process [PMID:19369424]
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
Ribokinase, phosphorylates ribose to ribose-5-phosphate; required for recycling ribose during nucleotide metabolism
ARS313; Autonomously Replicating Sequence on Chromosome III
SPX domain-containing inorganic phosphate transporter
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:21143198]
EXISTENCE:genetic interaction:GO:0005315 inorganic phosphate transmembrane transporter activity [PMID:11779791]
EXISTENCE:genetic interaction:GO:0006817 phosphate ion transport [PMID:11779791]
EXISTENCE:genetic interaction:GO:2000185 regulation of phosphate transmembrane transport [PMID:12821119]
EXISTENCE:mutant phenotype:GO:0006797 polyphosphate metabolic process [PMID:17804816]
EXISTENCE:mutant phenotype:GO:0097080 plasma membrane selenite transport [PMID:20688911]
Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of PHO pathway; targeted to vacuole via AP-3 pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
ARS314; Autonomously Replicating Sequence on Chromosome III; active in <10% of cell cycles
Ras family guanine nucleotide exchange factor BUD5
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11313501]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11313501]
EXISTENCE:genetic interaction:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:11589573|PMID:8234337]
EXISTENCE:mutant phenotype:GO:0007120 axial cellular bud site selection [PMID:1905981]
EXISTENCE:mutant phenotype:GO:0007121 bipolar cellular bud site selection [PMID:1905981]
GTP/GDP exchange factor for Rsr1p (Bud1p); required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types
MATALPHA; Mating type locus; note that the systematic sequence in SGD contains MATalpha sequences
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:3893743]
EXISTENCE:direct assay:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:15598821]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1976249]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:7569974|PMID:7739902]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:3893743]
Homeobox-domain protein; with Mcm1p, represses a-specific genes in haploids; acts with A1p to repress transcription of haploid-specific genes in diploids; one of two genes encoded by the MATalpha mating type cassette
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:6337727]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:15118075]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:6337727]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:6337727]
EXISTENCE:mutant phenotype:GO:0007532 regulation of mating-type specific transcription, DNA-templated [PMID:6337727]
Transcriptional co-activator that regulates mating-type-specific genes; targets the transcription factor Mcm1p to the promoters of alpha-specific genes; one of two genes encoded by the MATalpha mating type cassette
hypothetical protein; overexpression suppresses the high-frequency loss of mini-chromosomes, probably by increasing the rate of proper chromosome segregation; translated gene product of YCR041W, but not its transcript, is responsible for suppression; suppression ability of YCR041W is completely dependent on silencing protein Sir4p
EXISTENCE:curator inference:GO:0016251 RNA polymerase II general transcription initiation factor activity [PMID:15448131]
EXISTENCE:curator inference:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:10788514]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10818000|PMID:12600943]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
TFIID subunit (150 kDa); involved in RNA polymerase II transcription initiation
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:9560393]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17021251]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:17021251]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:9560393]
Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:19779569]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:19779569]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:19779569]
Probable subtilisin-family protease; role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores
mitochondrial 54S ribosomal protein IMG1
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome
18S rRNA (guanine1575-N7)-methyltransferase
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:18332120]
EXISTENCE:mutant phenotype:GO:0000056 ribosomal small subunit export from nucleus [PMID:18332120]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:18332120]
EXISTENCE:mutant phenotype:GO:0016435 rRNA (guanine) methyltransferase activity [PMID:18332120]
EXISTENCE:mutant phenotype:GO:0070476 rRNA (guanine-N7)-methylation [PMID:18332120]
Ribosome biogenesis factor; methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; required for recruitment of Rps2p; functions with DEAH-box RNA helicase Ecm16p; diploid mutant displays random budding pattern; functional homolog of human WBSCR22
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10672016|PMID:26928762]
EXISTENCE:genetic interaction:GO:0034737 ergosterol O-acyltransferase activity [PMID:10672016]
EXISTENCE:genetic interaction:GO:0034738 lanosterol O-acyltransferase activity [PMID:10672016]
EXISTENCE:mutant phenotype:GO:0016125 sterol metabolic process [PMID:10672016]
EXISTENCE:mutant phenotype:GO:0034738 lanosterol O-acyltransferase activity [PMID:10672016]
Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
Non-essential hypothetical protein; deletion mutant is synthetically sick or lethal with alpha-synuclein
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:31886770]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:31886770]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:17081996]
Component of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:24390141]
EXISTENCE:mutant phenotype:GO:0004795 threonine synthase activity [PMID:8082795]
EXISTENCE:mutant phenotype:GO:0009088 threonine biosynthetic process [PMID:8082795]
Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
Essential hypothetical protein; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
snoRNA-binding rRNA-processing protein PWP2
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12068309|PMID:12150911]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8804409]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:15231838|PMID:12150911]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:12068309]
EXISTENCE:direct assay:GO:0034388 Pwp2p-containing subcomplex of 90S preribosome [PMID:15231838|PMID:17515605]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15231838|PMID:15489292|PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:12068309]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15231838|PMID:15489292|PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15489292|PMID:15590835|PMID:15231838]
EXISTENCE:mutant phenotype:GO:0000920 septum digestion after cytokinesis [PMID:8804409]
EXISTENCE:mutant phenotype:GO:0030010 establishment of cell polarity [PMID:8804409]
Conserved 90S pre-ribosomal component; essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
EXISTENCE:direct assay:GO:0003785 actin monomer binding [PMID:15126500]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:22404850]
EXISTENCE:direct assay:GO:1905538 polysome binding [PMID:22404850]
EXISTENCE:mutant phenotype:GO:0001933 negative regulation of protein phosphorylation [PMID:15126500]
EXISTENCE:mutant phenotype:GO:0004860 protein kinase inhibitor activity [PMID:15126500]
Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
EXISTENCE:direct assay:GO:0051087 chaperone binding [PMID:18412542]
EXISTENCE:direct assay:GO:0097255 R2TP complex [PMID:15766533]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:mutant phenotype:GO:0000492 box C/D snoRNP assembly [PMID:18268103]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:mutant phenotype:GO:0051087 chaperone binding [PMID:15766533]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:15766533]
EXISTENCE:physical interaction:GO:0051087 chaperone binding [PMID:15766533]
EXISTENCE:physical interaction:GO:0097255 R2TP complex [PMID:18268103]
Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants
ARS315; Highly-active autonomously replicating sequence; initiates replication in 90% of cell cycles
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; non-essential gene; upregulated by and required for tolerance to 4-Methylcyclohexane methanol; induced by treatment with 8-methoxypsoralen and UVA irradiation; contains 10-11 predicted transmembrane domains
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis; i6A37 modification is catalyzed by Mod5p; can mutate to suppress amber or ochre nonsense mutations; suppressor mutant alleles are recessive lethal and can only be maintained when an additional wild-type copy of the gene is present, because SUP61 encodes the only tRNA species that can decode UCG codons
EXISTENCE:direct assay:GO:0005686 U2 snRNP [PMID:16314500]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:11452010]
EXISTENCE:physical interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:16314500|PMID:15303280]
Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:12464632]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:16912276]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19159085|PMID:20847055]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20847055]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:33339134]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8441413]
EXISTENCE:genetic interaction:GO:0051300 spindle pole body organization [PMID:9920414]
EXISTENCE:mutant phenotype:GO:0003700 DNA-binding transcription factor activity [PMID:16912276]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:20847055]
EXISTENCE:mutant phenotype:GO:0034246 mitochondrial transcription factor activity [PMID:33339134]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:20847055]
EXISTENCE:mutant phenotype:GO:1903108 regulation of mitochondrial transcription [PMID:33339134]
Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources, NAD synthesis, oxidative stress resistance; regulated by cell wall integrity checkpoint; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4
EXISTENCE:direct assay:GO:0000785 chromatin [PMID:10880451]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:7926769|PMID:9287349]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10880451]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:9287349]
EXISTENCE:direct assay:GO:0097505 Rad6-Rad18 complex [PMID:9287349]
EXISTENCE:genetic interaction:GO:0042275 error-free postreplication DNA repair [PMID:10924462|PMID:9576943]
EXISTENCE:genetic interaction:GO:0042276 error-prone translesion synthesis [PMID:11973297|PMID:15687278]
EXISTENCE:genetic interaction:GO:0070987 error-free translesion synthesis [PMID:9409821]
EXISTENCE:mutant phenotype:GO:0006513 protein monoubiquitination [PMID:12226657]
E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
EXISTENCE:direct assay:GO:0003400 regulation of COPII vesicle coating [PMID:21291503]
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:21291503]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:7593162]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:21291503]
EXISTENCE:physical interaction:GO:0043547 positive regulation of GTPase activity [PMID:21291503]
Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004620 phospholipase activity [PMID:27543826]
EXISTENCE:direct assay:GO:0005775 vacuolar lumen [PMID:11566994]
EXISTENCE:direct assay:GO:0006624 vacuolar protein processing [PMID:11085977]
EXISTENCE:direct assay:GO:0006660 phosphatidylserine catabolic process [PMID:27543826]
EXISTENCE:direct assay:GO:0006914 autophagy [PMID:11085977|PMID:11566994]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:11085977]
EXISTENCE:direct assay:GO:0030397 membrane disassembly [PMID:11085977]
EXISTENCE:mutant phenotype:GO:0000425 pexophagy [PMID:31332264]
EXISTENCE:mutant phenotype:GO:0004620 phospholipase activity [PMID:27543826]
EXISTENCE:mutant phenotype:GO:0006624 vacuolar protein processing [PMID:11085977]
EXISTENCE:mutant phenotype:GO:0006660 phosphatidylserine catabolic process [PMID:27543826]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:11085977|PMID:11566994]
EXISTENCE:mutant phenotype:GO:0030397 membrane disassembly [PMID:11085977]
EXISTENCE:mutant phenotype:GO:0034496 multivesicular body membrane disassembly [PMID:12499386]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0046461 neutral lipid catabolic process [PMID:26061644]
Phospholipase; preferentially hydrolyses phosphatidylserine, with minor activity against cardiolipin and phosphatidylethanolamine; required for lysis of autophagic and CVT bodies; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; targeted to intravacuolar vesicles during autophagy via multivesicular body (MVB) pathway; required for maintenance of lipid droplet quantity after diauxic shift; regulates lipolysis; expression regulated by Yap1p during autophagy
peptidylprolyl isomerase family protein CPR4
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication
mitochondrial 54S ribosomal protein IMG2
Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:16221974]
EXISTENCE:mutant phenotype:GO:0000027 ribosomal large subunit assembly [PMID:16221974]
EXISTENCE:mutant phenotype:GO:0110136 protein-RNA complex remodeling [PMID:19737519]
Ribosomal large subunit (60S) assembly factor; association of the N-terminal ubiquitin-like (UBL) domain of this preribosomal factor with the MIDAS domain in the Rea1p tail, promoted by the GTPase Nog2p, is required for the ATP-dependent dissociation of preribosomal factors including the Rix1p subcomplex, Rea1p, Nog2p and Rsa1p itself prior to nuclear export of pre-60S particles; contains WD-repeats and a UBL domain; localizes to the nucleolus
mitogen-activated protein kinase kinase kinase SSK22
EXISTENCE:genetic interaction:GO:0000161 osmosensory signaling MAPK cascade [PMID:7624781|PMID:9742096]
EXISTENCE:mutant phenotype:GO:0000161 osmosensory signaling MAPK cascade [PMID:7624781]
MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15454531]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:15454531]
Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:15885099|PMID:27142334]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:15885099]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27142334]
EXISTENCE:direct assay:GO:0015811 L-cystine transport [PMID:27142334]
EXISTENCE:genetic interaction:GO:0015184 L-cystine transmembrane transporter activity [PMID:15885099]
EXISTENCE:genetic interaction:GO:0015811 L-cystine transport [PMID:15885099]
Protein involved in cystine transport; localizes to the vacuole, plasma membrane and endosome; similarity to human cystinosin, a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains; mutation is functionally complemented by human CTNS
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26206314]
EXISTENCE:mutant phenotype:GO:0038202 TORC1 signaling [PMID:26206314]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:26206314]
EXISTENCE:physical interaction:GO:0071986 Ragulator complex [PMID:26206314]
Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; identified by homology to Ashbya gossypii; EGO2 has a paralog, EGO4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:22362029]
Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; specifically blocks all three proteasome active sites; exhibits boundary activity which inhibits the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:23893485]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:12730603]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:23706738]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:25035297]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23706738]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23706738|PMID:22842922]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23222640]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:10779343]
EXISTENCE:direct assay:GO:0045947 negative regulation of translational initiation [PMID:20832728]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:genetic interaction:GO:0033962 P-body assembly [PMID:18981231]
EXISTENCE:mutant phenotype:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:10913177]
EXISTENCE:mutant phenotype:GO:0000932 P-body [PMID:12730603]
EXISTENCE:mutant phenotype:GO:0001731 formation of translation preinitiation complex [PMID:20832728]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:10779343]
EXISTENCE:mutant phenotype:GO:0033962 P-body assembly [PMID:18981231]
EXISTENCE:mutant phenotype:GO:0045947 negative regulation of translational initiation [PMID:20832728]
EXISTENCE:physical interaction:GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA [PMID:10913177]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:26952104]
Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:17002782]
EXISTENCE:direct assay:GO:0004741 [pyruvate dehydrogenase (lipoamide)] phosphatase activity [PMID:28539364]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:14562095]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:17166847]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:18180296]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:17166847]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:17166847]
EXISTENCE:mutant phenotype:GO:0004741 [pyruvate dehydrogenase (lipoamide)] phosphatase activity [PMID:18180296]
EXISTENCE:mutant phenotype:GO:0070262 peptidyl-serine dephosphorylation [PMID:18180296]
EXISTENCE:mutant phenotype:GO:1901524 regulation of mitophagy [PMID:31548421]
EXISTENCE:mutant phenotype:GO:1904184 positive regulation of pyruvate dehydrogenase activity [PMID:18180296]
Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:20505076]
EXISTENCE:direct assay:GO:0016592 mediator complex [PMID:11383511|PMID:12200444]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16109375]
EXISTENCE:mutant phenotype:GO:0000411 positive regulation of transcription by galactose [PMID:15601835]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:20505076]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:15601835]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16464826]
EXISTENCE:direct assay:GO:0140110 transcription regulator activity [PMID:16464826]
EXISTENCE:physical interaction:GO:0016573 histone acetylation [PMID:21734642]
Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:10037727|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0015036 disulfide oxidoreductase activity [PMID:10037727]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:10037727]
Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
AAR1; AER2; AMM1; CRT4; CYC9; FLK1; ROX4; SFL2; UMR7
chromatin-silencing transcriptional regulator TUP1
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9741624]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1739976]
EXISTENCE:direct assay:GO:0036033 mediator complex binding [PMID:11226276]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:8675011]
EXISTENCE:direct assay:GO:0042826 histone deacetylase binding [PMID:14525981]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:21536737]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18708352|PMID:1900249|PMID:9858577|PMID:9560430|PMID :1312982]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:9461460]
EXISTENCE:mutant phenotype:GO:0003714 transcription corepressor activity [PMID:9741624]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9280286]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26546823]
EXISTENCE:mutant phenotype:GO:2001020 regulation of response to DNA damage stimulus [PMID:9741624]
EXISTENCE:physical interaction:GO:0017053 transcription repressor complex [PMID:9111019]
EXISTENCE:physical interaction:GO:0036033 mediator complex binding [PMID:11226276]
EXISTENCE:physical interaction:GO:0042826 histone deacetylase binding [PMID:14525981]
General repressor of transcription in complex with Cyc8p; involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4 and stabilization of nucleosomes over promoters; appears to enhance expression of mating-related genes; functions as a coactivator in certain situations
hypothetical protein; conserved among S. cerevisiae strains; YCR085W is not an essential gene
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11489916]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12689592]
EXISTENCE:direct assay:GO:0033551 monopolin complex [PMID:12689592]
EXISTENCE:genetic interaction:GO:0051455 monopolar spindle attachment to meiosis I kinetochore [PMID:12689592]
EXISTENCE:mutant phenotype:GO:0034503 protein localization to nucleolar rDNA repeats [PMID:19362534]
EXISTENCE:mutant phenotype:GO:0045132 meiotic chromosome segregation [PMID:11470404]
EXISTENCE:mutant phenotype:GO:0045143 homologous chromosome segregation [PMID:12689592]
EXISTENCE:mutant phenotype:GO:0070550 rDNA chromatin condensation [PMID:18923139]
EXISTENCE:physical interaction:GO:0045143 homologous chromosome segregation [PMID:12689592]
Nucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11950888]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:3060468|PMID:8163554|PMID:16824951]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:3060468]
EXISTENCE:direct assay:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:16824951]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:18177206]
EXISTENCE:mutant phenotype:GO:0044379 protein localization to actin cortical patch [PMID:10087264|PMID:11668184|PMID:15798181]
EXISTENCE:mutant phenotype:GO:0051016 barbed-end actin filament capping [PMID:23333351]
Actin-binding protein of the cortical actin cytoskeleton; important for activation of actin nucleation mediated by the Arp2/Arp3 complex; inhibits actin filament elongation at the barbed-end; phosphorylation within its proline-rich region, mediated by Cdc28p and Pho85p, protects Abp1p from PEST sequence-mediated proteolysis; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa)
EXISTENCE:direct assay:GO:0005937 mating projection [PMID:11027318]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9456310]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:9456310]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9456310]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:11027318]
EXISTENCE:mutant phenotype:GO:0044182 filamentous growth of a population of unicellular organisms [PMID:11027318]
Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
ARS316; Inefficient autonomously replicating sequence; active in 25% of cell cycles
putative serine/threonine protein kinase KIN82
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:18199685]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:11337509]
Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000403 Y-form DNA binding [PMID:16781730]
EXISTENCE:direct assay:GO:0000404 heteroduplex DNA loop binding [PMID:16781730]
EXISTENCE:direct assay:GO:0000406 double-strand/single-strand DNA junction binding [PMID:16781730]
EXISTENCE:direct assay:GO:0030983 mismatched DNA binding [PMID:17636021]
EXISTENCE:direct assay:GO:0032135 DNA insertion or deletion binding [PMID:8805366]
EXISTENCE:genetic interaction:GO:0000735 removal of nonhomologous ends [PMID:9256462]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:16702432]
EXISTENCE:mutant phenotype:GO:0000735 removal of nonhomologous ends [PMID:9256462]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:8510668|PMID:9520271]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:15920474]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:8849883]
EXISTENCE:mutant phenotype:GO:0043111 replication fork arrest [PMID:27045900]
EXISTENCE:physical interaction:GO:0032302 MutSbeta complex [PMID:8805366]
Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
EXISTENCE:direct assay:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11889048]
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:27692063]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:7926748]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0010607 negative regulation of cytoplasmic mRNA processing body assembly [PMID:27692063]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:mutant phenotype:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:2099190]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:9055077]
EXISTENCE:mutant phenotype:GO:0010607 negative regulation of cytoplasmic mRNA processing body assembly [PMID:27692063]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:2680756]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10490603]
EXISTENCE:physical interaction:GO:0030015 CCR4-NOT core complex [PMID:10490603]
Subunit of the CCR4-NOT1 core complex; this complex has multiple roles in the regulation of mRNA levels including regulation of transcription and destabilization of mRNA by deadenylation; basal transcription factor that increases initiation and elongation; activates the ATPase activity of Dhh1p, resulting in processing body disassembly
aminophospholipid translocase regulatory protein CDC50
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:15090616]
EXISTENCE:direct assay:GO:0031902 late endosome membrane [PMID:12589066]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:16195350]
EXISTENCE:genetic interaction:GO:0015247 aminophospholipid flippase activity [PMID:15090616]
EXISTENCE:genetic interaction:GO:0045332 phospholipid translocation [PMID:15090616]
EXISTENCE:genetic interaction:GO:0051666 actin cortical patch localization [PMID:16195350]
EXISTENCE:mutant phenotype:GO:0006886 intracellular protein transport [PMID:16956384]
EXISTENCE:mutant phenotype:GO:0042147 retrograde transport, endosome to Golgi [PMID:28057802]
EXISTENCE:physical interaction:GO:1990530 Cdc50p-Drs2p complex [PMID:15090616]
Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
HMR; Mating-type cassette - right; unexpressed complete copy of the mating-type genes, silenced by the Sir2 histone deacetylase and its associated SIR1, SIR3 and SIR4 proteins; mating-type switching involves replacement of the Ya or Yalpha sequences present at the MAT locus with either the a or alpha genes located at one of the HM loci in a process stimulated by the HO endonuclease; sequence replacement occurs by gene conversion, leaving the donor unaltered
ARS317; ARS that fires extremely inefficiently and late during S phase; fires late and inefficiently compared to other origins in the genome; active in only a small fraction of cell cycles
Silenced copy of a2 at HMR; similarity to Alpha2p; required along with a1p for inhibiting expression of the HO endonuclease in a/alpha HO/HO diploid cells with an active mating-type interconversion system
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:1977088]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8411150]
EXISTENCE:direct assay:GO:0007532 regulation of mating-type specific transcription, DNA-templated [PMID:1977088]
Silenced copy of a1 at HMR; homeobox corepressor that interacts with Alpha2p to repress haploid-specific gene transcription in diploid cells
ARS318; ARS that becomes active if the nearby ARS317/HMR-E silencer is deleted; active in only a small fraction of cell cycles
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0001406 glycerophosphodiester transmembrane transporter activity [PMID:16924500]
EXISTENCE:direct assay:GO:0001407 glycerophosphodiester transmembrane transport [PMID:16924500]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16924500]
EXISTENCE:genetic interaction:GO:0015169 glycerol-3-phosphate transmembrane transporter activity [PMID:20133652]
EXISTENCE:genetic interaction:GO:0015794 glycerol-3-phosphate transmembrane transport [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0001406 glycerophosphodiester transmembrane transporter activity [PMID:9691030]
EXISTENCE:mutant phenotype:GO:0001407 glycerophosphodiester transmembrane transport [PMID:9691030]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:9691030]
EXISTENCE:mutant phenotype:GO:0015169 glycerol-3-phosphate transmembrane transporter activity [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0015794 glycerol-3-phosphate transmembrane transport [PMID:20133652]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9691030]
Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
Protein involved in protein sorting; involved in targeting mitochondrial membrane proteins to mitochondrial translocation system; mutation causes respiration defects
hypothetical protein; localizes to the membrane fraction; YCR101C is not an essential gene
EXISTENCE:direct assay:GO:0071468 cellular response to acidic pH [PMID:31374572]
EXISTENCE:mutant phenotype:GO:0046688 response to copper ion [PMID:15886332]
Putative quinone oxidoreductase; overexpression improves ethanol production and acid stress resistance; involved in copper metabolism; similar to Cochliobolus carbonum toxD gene
Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
EXISTENCE:direct assay:GO:0006066 alcohol metabolic process [PMID:12423374]
EXISTENCE:direct assay:GO:0008106 alcohol dehydrogenase (NADP+) activity [PMID:12423374]
NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance
EXISTENCE:mutant phenotype:GO:0009410 response to xenobiotic stimulus [PMID:11943786]
Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD3 has a paralog, AAD15, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
TEL03R; Telomeric region on the right arm of Chromosome III; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats
ARS319; Highly-active subtelomeric autonomously replicating sequence; initiates replication in 90% of cell cycles
hypothetical protein; identified by fungal homology and RT-PCR