Saccharomyces cerevisiae S288C chromosome II, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
Complete DNA sequence of yeast chromosome II
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006936.\n\nOn Apr 26, 2011 this sequence version replaced NC_001134.7.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL02L; Telomeric region on the left arm of Chromosome II; annotated components include an X element core sequence, X element combinatorial repeats, and a short Y' element; TEL02L does have telomeric repeats (TEL02L-TR), but they are missing from the genome annotation due to difficulties encountered during sequencing and/or assembly
ARS200; Autonomously replicating sequence
Helicase-like protein encoded within the telomeric Y' element
hypothetical protein; YBL112C is contained within TEL02L
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:22842922]
Helicase-like protein encoded within the telomeric Y' element; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
ARS201; Autonomously replicating sequence
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:22984289]
Mitochondrial intermembrane space hypothetical protein; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
putative Rab GTPase-binding protein SRO77
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:26928762]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:10402465]
EXISTENCE:genetic interaction:GO:0006893 Golgi to plasma membrane transport [PMID:10402465]
EXISTENCE:physical interaction:GO:0005886 plasma membrane [PMID:10402465]
EXISTENCE:physical interaction:GO:0006893 Golgi to plasma membrane transport [PMID:10402465]
Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004697 protein kinase C activity [PMID:26179915|PMID:8207005]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11545731]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11545731]
EXISTENCE:direct assay:GO:0005856 cytoskeleton [PMID:15910746]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:32184286]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:10893184]
EXISTENCE:direct assay:GO:0032165 prospore septin filament array [PMID:24390141]
EXISTENCE:direct assay:GO:0034063 stress granule assembly [PMID:32184286]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:7874200]
EXISTENCE:mutant phenotype:GO:0010606 positive regulation of cytoplasmic mRNA processing body assembly [PMID:21163942]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:20385774]
EXISTENCE:mutant phenotype:GO:0032186 cellular bud neck septin ring organization [PMID:26179915]
EXISTENCE:mutant phenotype:GO:0035556 intracellular signal transduction [PMID:7874200]
EXISTENCE:mutant phenotype:GO:0060211 regulation of nuclear-transcribed mRNA poly(A) tail shortening [PMID:21163942]
EXISTENCE:mutant phenotype:GO:0060237 regulation of fungal-type cell wall organization [PMID:7874200]
Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
EXISTENCE:direct assay:GO:0035859 Seh1-associated complex [PMID:21454883]
EXISTENCE:genetic interaction:GO:1904263 positive regulation of TORC1 signaling [PMID:23716719]
Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
EXISTENCE:direct assay:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9032238]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10848632]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10848632]
EXISTENCE:direct assay:GO:0031930 mitochondria-nucleus signaling pathway [PMID:9032238]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19158363]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9032238|PMID:9242640]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19840933]
EXISTENCE:mutant phenotype:GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific [PMID:9032238]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12393187|PMID:9032238|PMID:9242640]
EXISTENCE:mutant phenotype:GO:0071400 cellular response to oleic acid [PMID:12069115|PMID:12393187]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:9867831]
bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:7596416]
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:10406798|PMID:9725919]
EXISTENCE:genetic interaction:GO:0042147 retrograde transport, endosome to Golgi [PMID:10406798]
Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0070086 ubiquitin-dependent endocytosis [PMID:18976803|PMID:32744498|PMID:31658248]
EXISTENCE:physical interaction:GO:0031625 ubiquitin protein ligase binding [PMID:18976803]
EXISTENCE:physical interaction:GO:0070086 ubiquitin-dependent endocytosis [PMID:18976803]
Alpha-arrestin, ubiquitin ligase adaptor for Rsp5p; regulates starvation- and substrate-induced Ub-dependent endocytosis of select plasma membrane localized amino acid transporters; substrate of Rsp5p-mediated Lys63-polyubiquitination and Ubp2-mediated deubiquitination; promoter contains several Gcn4p binding sites required for induction in response to starvation via Gcn4p and the general amino acid control pathway; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
ARS230; Autonomously Replicating Sequence
YBLWTy2-1; Ty2 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:10024171]
Phenylalanine tRNA (tRNA-Phe), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11502169|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core [PMID:15294286|PMID:17082766]
EXISTENCE:direct assay:GO:0015986 proton motive force-driven ATP synthesis [PMID:20691145]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:18722382]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:10869431]
EXISTENCE:direct assay:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:20691145]
EXISTENCE:mutant phenotype:GO:0005754 mitochondrial proton-transporting ATP synthase, catalytic core [PMID:2876995]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:2876995]
EXISTENCE:mutant phenotype:GO:0046933 proton-transporting ATP synthase activity, rotational mechanism [PMID:1827117|PMID:2876995]
Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:genetic interaction:GO:0034354 'de novo' NAD biosynthetic process from tryptophan [PMID:12062417]
EXISTENCE:mutant phenotype:GO:0004502 kynurenine 3-monooxygenase activity [PMID:15806102]
Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; localizes to mitochondria and peroxisomes; putative therapeutic target for Huntington disease
EXISTENCE:direct assay:GO:0000796 condensin complex [PMID:10811823]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10749931]
EXISTENCE:genetic interaction:GO:0003682 chromatin binding [PMID:11864994]
EXISTENCE:mutant phenotype:GO:0000070 mitotic sister chromatid segregation [PMID:10749931]
EXISTENCE:mutant phenotype:GO:0007076 mitotic chromosome condensation [PMID:10749931|PMID:10811823|PMID:7698648]
EXISTENCE:mutant phenotype:GO:0070058 tRNA gene clustering [PMID:18708579]
Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:25546499]
Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1656237]
EXISTENCE:direct assay:GO:0070847 core mediator complex [PMID:9891034]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0001113 transcription open complex formation at RNA polymerase II promoter [PMID:20048049]
EXISTENCE:mutant phenotype:GO:0003713 transcription coactivator activity [PMID:15831453]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16452140]
EXISTENCE:mutant phenotype:GO:0051123 RNA polymerase II preinitiation complex assembly [PMID:15964818]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:11983894]
EXISTENCE:curator inference:GO:0003735 structural constituent of ribosome [PMID:11983894]
EXISTENCE:direct assay:GO:0022625 cytosolic large ribosomal subunit [PMID:11983894]
Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
phospholipid metabolism-regulating protein SCS22
EXISTENCE:genetic interaction:GO:0008654 phospholipid biosynthetic process [PMID:15668246]
EXISTENCE:genetic interaction:GO:0060304 regulation of phosphatidylinositol dephosphorylation [PMID:23237950]
EXISTENCE:genetic interaction:GO:0061709 reticulophagy [PMID:35101986]
EXISTENCE:genetic interaction:GO:0090158 endoplasmic reticulum membrane organization [PMID:23237950]
Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect the ER and plasma membrane (PM); regulates PM PI4P levels by controlling access of the Sac1p phosphatase to its substrate, PI4P; human VAP homolog; similar to D. melanogaster inturned protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; SCS22 has a paralog, SCS2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:genetic interaction:GO:0070006 metalloaminopeptidase activity [PMID:8618900]
EXISTENCE:mutant phenotype:GO:0035551 protein initiator methionine removal involved in protein maturation [PMID:11811952]
Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
mitochondrial 37S ribosomal protein MRP21
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:9528754]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:9528754]
EXISTENCE:genetic interaction:GO:0070124 mitochondrial translational initiation [PMID:9528754]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:9528754]
Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:3513174|PMID:7569949]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:11095737]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0042162 telomeric DNA binding [PMID:17656141]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:8604297]
EXISTENCE:genetic interaction:GO:0006281 DNA repair [PMID:25628362]
EXISTENCE:genetic interaction:GO:0006302 double-strand break repair [PMID:17347674]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:3513174|PMID:8604297]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:25329891]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:17347674]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:17347674]
EXISTENCE:mutant phenotype:GO:0042770 signal transduction in response to DNA damage [PMID:17693258]
Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene
Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
ARS202; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:12097146|PMID:28751498]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12097146]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12097146|PMID:22842922]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:12097146]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:19053807]
EXISTENCE:genetic interaction:GO:0000920 septum digestion after cytokinesis [PMID:16615892]
EXISTENCE:genetic interaction:GO:0007015 actin filament organization [PMID:31964708]
EXISTENCE:genetic interaction:GO:0007118 budding cell apical bud growth [PMID:17417630]
EXISTENCE:mutant phenotype:GO:0099500 vesicle fusion to plasma membrane [PMID:28751498]
Protein involved in polar growth, functionally redundant with Boi2p; required for fusion of secretory vesicles with plasma membrane at sites of polarized growth; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
anaphase promoting complex subunit CDC27
EXISTENCE:direct assay:GO:0005680 anaphase-promoting complex [PMID:9469814]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:direct assay:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:8895471]
EXISTENCE:direct assay:GO:0034399 nuclear periphery [PMID:25817432]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0031145 anaphase-promoting complex-dependent catabolic process [PMID:16481473]
EXISTENCE:mutant phenotype:GO:0061630 ubiquitin protein ligase activity [PMID:16481473]
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
EXISTENCE:direct assay:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:11308030]
EXISTENCE:direct assay:GO:0052925 dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity [PMID:11308030]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:8842708]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:8842708]
EXISTENCE:mutant phenotype:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:8842708]
Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
Non-essential hypothetical protein; null mutation results in a decrease in plasma membrane electron transport
glutamyl-tRNA(Gln) amidotransferase subunit PET112
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19417106|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex [PMID:19417106]
EXISTENCE:direct assay:GO:0050567 glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity [PMID:19417106]
EXISTENCE:direct assay:GO:0070681 glutaminyl-tRNAGln biosynthesis via transamidation [PMID:19417106]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:8082172]
Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
EXISTENCE:curator inference:GO:0006913 nucleocytoplasmic transport [PMID:10684247]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0044611 nuclear pore inner ring [PMID:18046406]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:23452847]
EXISTENCE:direct assay:GO:1990841 promoter-specific chromatin binding [PMID:23452847]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:19414608|PMID:20498018]
EXISTENCE:genetic interaction:GO:0051292 nuclear pore complex assembly [PMID:20498018|PMID:19414608]
EXISTENCE:mutant phenotype:GO:0003682 chromatin binding [PMID:23452847]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:11290711]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:12509452|PMID:19414606|PMID:17418788]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:23452847]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:23452847]
EXISTENCE:mutant phenotype:GO:0036228 protein localization to nuclear inner membrane [PMID:16929305|PMID:21659568]
Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and Nup157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10837468]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:11689437|PMID:15659643|PMID:16680092|PMID:23129774|P MID:19398890]
EXISTENCE:direct assay:GO:0005776 autophagosome [PMID:10525546|PMID:16079147]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:28003432]
EXISTENCE:direct assay:GO:0061025 membrane fusion [PMID:17632063]
EXISTENCE:direct assay:GO:0120095 vacuole-isolation membrane contact site [PMID:23549786]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10837468]
EXISTENCE:mutant phenotype:GO:0000422 autophagy of mitochondrion [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10837468]
EXISTENCE:mutant phenotype:GO:0008429 phosphatidylethanolamine binding [PMID:19398890]
EXISTENCE:mutant phenotype:GO:0016241 regulation of macroautophagy [PMID:19398890]
EXISTENCE:mutant phenotype:GO:0031386 protein tag [PMID:22652539]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:22885598]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:9649430|PMID:19398890]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:25680528]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:18701704]
EXISTENCE:mutant phenotype:GO:0044805 late nucleophagy [PMID:22768199]
EXISTENCE:mutant phenotype:GO:0061025 membrane fusion [PMID:17632063]
EXISTENCE:mutant phenotype:GO:0061709 reticulophagy [PMID:26181331]
EXISTENCE:mutant phenotype:GO:0071211 protein targeting to vacuole involved in autophagy [PMID:22885598]
EXISTENCE:mutant phenotype:GO:0140042 lipid droplet formation [PMID:28003432]
EXISTENCE:mutant phenotype:GO:1905153 regulation of membrane invagination [PMID:31616012]
EXISTENCE:physical interaction:GO:0000329 fungal-type vacuole membrane [PMID:10837468]
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10837468]
Ubiquitin-like protein conjugated to phosphatidylethanolamine (PE); role in membrane fusion and phagophore expansion during autophagosome formation; deconjugation is also required for biogenesis; targets Atg1p to autophagosomes; binds to the Atg1p-Atg13p complex, triggers its vacuolar degradation; role in vacuolar membrane protein turnover during early stationary phase; component of autophagosomes and Cvt vesicles; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0004822 isoleucine-tRNA ligase activity [PMID:7619074|PMID:782885]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:7619074]
EXISTENCE:direct assay:GO:0006428 isoleucyl-tRNA aminoacylation [PMID:782885]
Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:10745074]
EXISTENCE:genetic interaction:GO:0006457 protein folding [PMID:9789005]
EXISTENCE:genetic interaction:GO:0016887 ATP hydrolysis activity [PMID:3302682]
EXISTENCE:genetic interaction:GO:0051082 unfolded protein binding [PMID:9789005]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:24855027]
EXISTENCE:mutant phenotype:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:8754838]
ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005682 U5 snRNP [PMID:11720285]
EXISTENCE:mutant phenotype:GO:0000244 spliceosomal tri-snRNP complex assembly [PMID:11720285]
Component of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:10024243]
EXISTENCE:curator inference:GO:0031428 box C/D RNP complex [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0030562 rRNA 2'-O-ribose methylation guide activity [PMID:10024243]
EXISTENCE:mutant phenotype:GO:0031167 rRNA methylation [PMID:10024243]
C/D box small nucleolar RNA (snoRNA); guides 2'-O-methylation of small subunit (SSU) rRNA at position G1428
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:18782943]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:18782943]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:18782943]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911]
EXISTENCE:genetic interaction:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16246728]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005506 iron ion binding [PMID:18021800|PMID:34154323]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:18627462|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18627462|PMID:14562095]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:34154323]
EXISTENCE:direct assay:GO:0008270 zinc ion binding [PMID:18021800]
EXISTENCE:direct assay:GO:0016730 oxidoreductase activity, acting on iron-sulfur proteins as donors [PMID:18021800]
EXISTENCE:direct assay:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:34154323]
EXISTENCE:direct assay:GO:0045182 translation regulator activity [PMID:34154323]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:15485916]
Zn-ribbon protein that co-purifies with Dph1 and Dph2; forms a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs; forms a stable heterodimer with Ats1p
hypothetical protein; conserved among S. cerevisiae strains; YBL071C is not an essential gene
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11739806]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:11739806]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11739806]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:11739806]
EXISTENCE:genetic interaction:GO:0006612 protein targeting to membrane [PMID:7822420]
EXISTENCE:mutant phenotype:GO:0032596 protein transport into membrane raft [PMID:11739806]
EXISTENCE:physical interaction:GO:0032596 protein transport into membrane raft [PMID:11739806]
Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
ribose phosphate diphosphokinase subunit PRS4
EXISTENCE:direct assay:GO:0002189 ribose phosphate diphosphokinase complex [PMID:15280369]
EXISTENCE:genetic interaction:GO:0004749 ribose phosphate diphosphokinase activity [PMID:15280369]
EXISTENCE:genetic interaction:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:15280369]
EXISTENCE:mutant phenotype:GO:0006015 5-phosphoribose 1-diphosphate biosynthetic process [PMID:10212224]
EXISTENCE:physical interaction:GO:0031505 fungal-type cell wall organization [PMID:15470112]
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic
ARS203; Autonomously replicating sequence
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:11076031]
EXISTENCE:mutant phenotype:GO:0010995 free ubiquitin chain depolymerization [PMID:11076031]
Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
Putative transcription factor; has homolog in Kluyveromyces lactis
EXISTENCE:direct assay:GO:0008379 thioredoxin peroxidase activity [PMID:10681558|PMID:10821871]
EXISTENCE:direct assay:GO:0045454 cell redox homeostasis [PMID:10681558]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:15051715]
EXISTENCE:mutant phenotype:GO:0008379 thioredoxin peroxidase activity [PMID:10681558]
EXISTENCE:mutant phenotype:GO:0045454 cell redox homeostasis [PMID:10681558]
EXISTENCE:mutant phenotype:GO:0046686 response to cadmium ion [PMID:19332553]
EXISTENCE:mutant phenotype:GO:1903205 regulation of hydrogen peroxide-induced cell death [PMID:31389622]
Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:1618910]
EXISTENCE:genetic interaction:GO:0000070 mitotic sister chromatid segregation [PMID:7860634]
EXISTENCE:genetic interaction:GO:0000073 initial mitotic spindle pole body separation [PMID:1618897|PMID:1618910]
EXISTENCE:genetic interaction:GO:0003777 microtubule motor activity [PMID:1618910]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:9813090]
EXISTENCE:mutant phenotype:GO:0007019 microtubule depolymerization [PMID:19041752]
EXISTENCE:mutant phenotype:GO:0030543 2-micrometer plasmid partitioning [PMID:19364922]
EXISTENCE:mutant phenotype:GO:0090307 mitotic spindle assembly [PMID:9813090]
Kinesin-related motor protein; tracks plus-ends and stabilizes microtubules; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:15470103]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15470103|PMID:9314530|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:9234668]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:17142567|PMID:9234668]
Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
Arf family guanine nucleotide exchange factor YEL1
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17786213]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:17786213]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17786213|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0008104 protein localization [PMID:18397879]
EXISTENCE:mutant phenotype:GO:0010513 positive regulation of phosphatidylinositol biosynthetic process [PMID:18208507]
EXISTENCE:mutant phenotype:GO:0051666 actin cortical patch localization [PMID:18397879]
Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:19703468]
EXISTENCE:mutant phenotype:GO:0033108 mitochondrial respiratory chain complex assembly [PMID:19703468]
Protein involved in respiratory chain complex assembly or maintenance; protein of the mitochondrial intermembrane space; contains twin Cx9C motifs that can form coiled coil-helix-coiled-coil helix fold
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:16823961|PMID:26928762]
Putative mitochondrial hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; IAI11 has a paralog, YER093C-A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0036435 K48-linked polyubiquitin modification-dependent protein binding [PMID:28525741]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:20855502]
EXISTENCE:mutant phenotype:GO:0005977 glycogen metabolic process [PMID:7891699]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:23418575|PMID:7891699]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:21148305]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:7891699]
EXISTENCE:mutant phenotype:GO:0031134 sister chromatid biorientation [PMID:20483956]
EXISTENCE:mutant phenotype:GO:0034727 piecemeal microautophagy of the nucleus [PMID:20855502]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:15258615]
Substrate adaptor for Cdc48p; ubiquitin regulatory X (UBX) and Ub-associated (UBA) domain-containing protein that acts as a substrate recruiting cofactor; K48-specific Ub chain binding protein involved in ER-associated degradation (ERAD); positively regulates Glc7p protein phosphatase activity to promote growth and mitotic progression in complex with Cdc48p; regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C)
EXISTENCE:direct assay:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:12799450]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process [PMID:17082762]
EXISTENCE:genetic interaction:GO:0004045 aminoacyl-tRNA hydrolase activity [PMID:12475929]
EXISTENCE:mutant phenotype:GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process [PMID:17082762]
One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1
EXISTENCE:direct assay:GO:0004722 protein serine/threonine phosphatase activity [PMID:10580002]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12477803|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12477803|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:10580002]
Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004519 endonuclease activity [PMID:15657035]
EXISTENCE:direct assay:GO:0008296 3'-5'-exodeoxyribonuclease activity [PMID:15657035]
EXISTENCE:mutant phenotype:GO:0006309 apoptotic DNA fragmentation [PMID:15657035]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:15657035]
3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:19158363]
PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization
EXISTENCE:direct assay:GO:0004402 histone acetyltransferase activity [PMID:10817755]
EXISTENCE:direct assay:GO:0033100 NuA3 histone acetyltransferase complex [PMID:10817755|PMID:17157260]
EXISTENCE:direct assay:GO:1990467 NuA3a histone acetyltransferase complex [PMID:25104842]
EXISTENCE:direct assay:GO:1990468 NuA3b histone acetyltransferase complex [PMID:25104842]
EXISTENCE:mutant phenotype:GO:0004402 histone acetyltransferase activity [PMID:10817755]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:11731479]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11731479]
Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:23222640|PMID:14562095]
EXISTENCE:genetic interaction:GO:0000077 DNA damage checkpoint signaling [PMID:15024067]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:15024067]
EXISTENCE:physical interaction:GO:0000077 DNA damage checkpoint signaling [PMID:15024067]
Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347|PMID:26928762]
EXISTENCE:direct assay:GO:0001671 ATPase activator activity [PMID:10387016]
EXISTENCE:direct assay:GO:0005483 soluble NSF attachment protein activity [PMID:11994317]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8636207]
EXISTENCE:direct assay:GO:0035494 SNARE complex disassembly [PMID:18650938|PMID:9425154]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:8670830|PMID:9685264]
EXISTENCE:direct assay:GO:0048280 vesicle fusion with Golgi apparatus [PMID:1601878]
EXISTENCE:mutant phenotype:GO:0006914 autophagy [PMID:11694599]
EXISTENCE:physical interaction:GO:0005483 soluble NSF attachment protein activity [PMID:10387016]
EXISTENCE:physical interaction:GO:0031201 SNARE complex [PMID:10397773]
Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP
hypothetical protein, essential for stationary phase survival; not required for growth on nonfermentable carbon sources; possibly linked with vacuolar transport
hypothetical protein; conserved across S. cerevisiae strains; identified in a screen for mutants with increased levels of rDNA transcription
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:25366307]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11452010]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11452010|PMID:26928762]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10954428|PMID:25366307]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:16563434]
EXISTENCE:genetic interaction:GO:0032467 positive regulation of cytokinesis [PMID:25371407]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:10954428]
EXISTENCE:mutant phenotype:GO:0030968 endoplasmic reticulum unfolded protein response [PMID:16582425]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:25625208]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:25366307]
Endocytic adaptor; K63-specific Ub-chain binding protein involved in receptor endocytosis; scaffold protein that localizes to nascent endocytic sites after homo-oligomerization and organizes the endocytic machinery; role in autophagy of aberrant clathrin-mediated endocytosis protein condensates; binds membranes in a Ub-dependent manner; interacts with and functions upstream of Cmk2p to suppress stress-induced non-apoptotic cell death; putative cytokinesis regulator; homolog of mammalian Eps15
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16857015|PMID:19520826|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:19520826]
EXISTENCE:direct assay:GO:0030289 protein phosphatase 4 complex [PMID:16299494|PMID:17517611]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:16857015]
EXISTENCE:mutant phenotype:GO:0030289 protein phosphatase 4 complex [PMID:16299494]
EXISTENCE:mutant phenotype:GO:2000002 negative regulation of DNA damage checkpoint [PMID:16299494]
Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
ubiquinol--cytochrome-c reductase subunit COR1
EXISTENCE:direct assay:GO:0005750 mitochondrial respiratory chain complex III [PMID:10873857|PMID:3023384]
EXISTENCE:direct assay:GO:0008121 ubiquinol-cytochrome-c reductase activity [PMID:3023384]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:2174874]
Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain
hypothetical protein; YBL044W is not an essential protein
Non-essential hypothetical protein; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:26928762]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16854981|PMID:22842922]
EXISTENCE:direct assay:GO:0015213 uridine transmembrane transporter activity [PMID:16854981]
EXISTENCE:direct assay:GO:0015862 uridine transport [PMID:16854981]
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:10827169]
EXISTENCE:mutant phenotype:GO:0015213 uridine transmembrane transporter activity [PMID:10827169]
EXISTENCE:mutant phenotype:GO:0015862 uridine transport [PMID:10827169|PMID:16854981]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:10827169]
High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:26928762]
EXISTENCE:direct assay:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:19162040]
EXISTENCE:direct assay:GO:0019774 proteasome core complex, beta-subunit complex [PMID:9087403]
EXISTENCE:direct assay:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:11545745|PMID:19029916]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:2194670]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:2194671|PMID:9405360]
EXISTENCE:mutant phenotype:GO:0045015 HDEL sequence binding [PMID:2194670]
HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:26928762]
Mitochondrial hypothetical protein; mCherry fusion protein localizes to the vacuole
ARS206; Autonomously replicating sequence
EXISTENCE:direct assay:GO:0003883 CTP synthase activity [PMID:8075080]
EXISTENCE:direct assay:GO:0006241 CTP biosynthetic process [PMID:8075080]
EXISTENCE:direct assay:GO:0097268 cytoophidium [PMID:20713603]
EXISTENCE:mutant phenotype:GO:0003883 CTP synthase activity [PMID:1753946]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:9668079]
EXISTENCE:mutant phenotype:GO:0019856 pyrimidine nucleobase biosynthetic process [PMID:1753946]
Major CTP synthase isozyme (see also URA8); catalyzes ATP-dependent transfer of amide nitrogen from glutamine to UTP, forming CTP, final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; targeted to vacuole via AP-3 pathway; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has paralog URA8 that arose from the whole genome duplication
mitochondrial 54S ribosomal protein YmL47
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:7478995|PMID:9151978]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:7478995|PMID:9151978]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:7478995]
Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetic lethality with hac1 mutation suggests a possible role in synthesis of precursors for protein glycosylation
EXISTENCE:curator inference:GO:0006886 intracellular protein transport [PMID:10564262]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:19458192|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0030122 AP-2 adaptor complex [PMID:10564262]
Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
EXISTENCE:direct assay:GO:0030170 pyridoxal phosphate binding [PMID:12499548]
Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
DNA-directed DNA polymerase alpha subunit POL12
EXISTENCE:curator inference:GO:0006273 lagging strand elongation [PMID:3888995]
EXISTENCE:direct assay:GO:0003887 DNA-directed DNA polymerase activity [PMID:1704371|PMID:3888995]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8289832|PMID:15282802]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:15282802]
EXISTENCE:direct assay:GO:0005658 alpha DNA polymerase:primase complex [PMID:22593576|PMID:3888995]
EXISTENCE:genetic interaction:GO:0016233 telomere capping [PMID:15132993]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:8289832]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:15132993]
EXISTENCE:physical interaction:GO:0016233 telomere capping [PMID:15132993]
B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:19952112]
EXISTENCE:direct assay:GO:0005827 polar microtubule [PMID:19952112]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:12058056]
EXISTENCE:direct assay:GO:0043515 kinetochore binding [PMID:19952112]
EXISTENCE:direct assay:GO:0048487 beta-tubulin binding [PMID:12058056]
EXISTENCE:direct assay:GO:0072686 mitotic spindle [PMID:19952112|PMID:7806575]
EXISTENCE:direct assay:GO:1990023 mitotic spindle midzone [PMID:19952112]
EXISTENCE:mutant phenotype:GO:0008017 microtubule binding [PMID:12058056]
EXISTENCE:mutant phenotype:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:19952112]
EXISTENCE:mutant phenotype:GO:0048487 beta-tubulin binding [PMID:12058056]
EXISTENCE:mutant phenotype:GO:0090307 mitotic spindle assembly [PMID:7806575]
Microtubule plus-end-tracking non-motor protein; binds to MTs, stabilizing interpolar MT plus ends, facilitating polymerization of spindle MTs and producing an outward force on spindle poles; required for structural integrity of the mitotic spindle; binds detached kinetochores (KTs) and promotes capture; relocalizes to MTs and stabilizes the spindle once captured KTs reach a spindle pole; localizes to the spindle, spindles poles, and detached KTs; CLASP family member homologous to human CLASP1/2
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:4555411|PMID:5366000]
EXISTENCE:mutant phenotype:GO:0019238 cyclohydrolase activity [PMID:4555411]
GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:11739741]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:20953064|PMID:23222640]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:11867544|PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11867544]
EXISTENCE:direct assay:GO:0008298 intracellular mRNA localization [PMID:11867544]
EXISTENCE:genetic interaction:GO:0000723 telomere maintenance [PMID:11739741]
EXISTENCE:genetic interaction:GO:0048255 mRNA stabilization [PMID:20953064]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:11739741]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:11867544]
EXISTENCE:mutant phenotype:GO:0048255 mRNA stabilization [PMID:20953064]
RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K; increases mRNA stability by binding (CNN)n repeat elements
EXISTENCE:direct assay:GO:0005819 spindle [PMID:17634282]
EXISTENCE:direct assay:GO:0005880 nuclear microtubule [PMID:17634282]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:17634282]
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:23142046]
EXISTENCE:direct assay:GO:0015630 microtubule cytoskeleton [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0000022 mitotic spindle elongation [PMID:18194531]
Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
EXISTENCE:direct assay:GO:0005471 ATP:ADP antiporter activity [PMID:8476415]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24769239|PMID:16823961]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:795470]
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:35127709]
EXISTENCE:direct assay:GO:0015866 ADP transport [PMID:8476415]
EXISTENCE:direct assay:GO:0015867 ATP transport [PMID:8476415|PMID:35127709]
EXISTENCE:direct assay:GO:0042626 ATPase-coupled transmembrane transporter activity [PMID:35127709]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:8476415]
EXISTENCE:genetic interaction:GO:0009061 anaerobic respiration [PMID:1915842]
EXISTENCE:mutant phenotype:GO:0006839 mitochondrial transport [PMID:2167309]
EXISTENCE:mutant phenotype:GO:0006915 apoptotic process [PMID:17822411]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:2167309]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:18728780]
Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; also functions in peroxisomes; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:17443350]
hypothetical protein that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
ARS207; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000398 mRNA splicing, via spliceosome [PMID:10377396]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18611963]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:15075370]
EXISTENCE:direct assay:GO:0005688 U6 snRNP [PMID:10369684|PMID:11720284]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15075370]
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419]
EXISTENCE:direct assay:GO:1990726 Lsm1-7-Pat1 complex [PMID:24139796]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:10428970]
EXISTENCE:physical interaction:GO:0005732 sno(s)RNA-containing ribonucleoprotein complex [PMID:15075370]
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0000500 RNA polymerase I upstream activating factor complex [PMID:8846924]
EXISTENCE:direct assay:GO:0001165 RNA polymerase I cis-regulatory region sequence-specific DNA binding [PMID:18451108]
EXISTENCE:direct assay:GO:0001181 RNA polymerase I general transcription initiation factor activity [PMID:8846924]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:22362748]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:8846924]
Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:17567576]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9767141]
EXISTENCE:direct assay:GO:0016428 tRNA (cytosine-5-)-methyltransferase activity [PMID:10445884]
EXISTENCE:direct assay:GO:0030488 tRNA methylation [PMID:10445884]
EXISTENCE:mutant phenotype:GO:0002127 tRNA wobble base cytosine methylation [PMID:22760636]
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:31416893]
EXISTENCE:mutant phenotype:GO:0019222 regulation of metabolic process [PMID:31416893]
EXISTENCE:mutant phenotype:GO:0070301 cellular response to hydrogen peroxide [PMID:22760636]
S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:10783164]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10704410|PMID:22842922|PMID:8224843]
EXISTENCE:direct assay:GO:0005656 nuclear pre-replicative complex [PMID:16824194|PMID:9335335]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10704410|PMID:22842922|PMID:8224843]
EXISTENCE:direct assay:GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication [PMID:16824194]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:18657510]
EXISTENCE:direct assay:GO:0031261 DNA replication preinitiation complex [PMID:9554851]
EXISTENCE:direct assay:GO:0031298 replication fork protection complex [PMID:16531994]
EXISTENCE:direct assay:GO:0042555 MCM complex [PMID:12480933]
EXISTENCE:direct assay:GO:0071162 CMG complex [PMID:26524492]
EXISTENCE:direct assay:GO:1905775 negative regulation of DNA helicase activity [PMID:13679365]
EXISTENCE:mutant phenotype:GO:0000727 double-strand break repair via break-induced replication [PMID:20516198]
EXISTENCE:mutant phenotype:GO:0003697 single-stranded DNA binding [PMID:17895243]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10834843]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:22564307]
EXISTENCE:mutant phenotype:GO:1902975 mitotic DNA replication initiation [PMID:9407029|PMID:8224843]
Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000262 mitochondrial chromosome [PMID:31532710]
EXISTENCE:genetic interaction:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:7957078]
EXISTENCE:genetic interaction:GO:0051131 chaperone-mediated protein complex assembly [PMID:8810243]
EXISTENCE:mutant phenotype:GO:0004176 ATP-dependent peptidase activity [PMID:8146662|PMID:8276800]
EXISTENCE:mutant phenotype:GO:0005759 mitochondrial matrix [PMID:8146662|PMID:8276800]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:16336126|PMID:7957078]
EXISTENCE:mutant phenotype:GO:1901858 regulation of mitochondrial DNA metabolic process [PMID:31532710]
ATP-dependent Lon protease; involved in degradation of misfolded mitochondrial proteins; required for mitochondrial maintenance and biogenesis; regulates mitochondrial DNA copy number with Mrx6p; subunit of a complex containing Mrx6p, Pet20p, and Mam33p that may regulate mtDNA replication; protease-independent, chaperone-like function in mitochondrial membrane complex assembly; localizes to the mitochondrial matrix
EXISTENCE:direct assay:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:2832951]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:7828851]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:2832951]
EXISTENCE:direct assay:GO:0016602 CCAAT-binding factor complex [PMID:2832951]
EXISTENCE:mutant phenotype:GO:0043457 regulation of cellular respiration [PMID:2182199]
EXISTENCE:physical interaction:GO:0016602 CCAAT-binding factor complex [PMID:2832951]
Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
EXISTENCE:genetic interaction:GO:0005789 endoplasmic reticulum membrane [PMID:11807558]
EXISTENCE:genetic interaction:GO:0034203 glycolipid translocation [PMID:11807558]
EXISTENCE:mutant phenotype:GO:0005789 endoplasmic reticulum membrane [PMID:11807558]
EXISTENCE:mutant phenotype:GO:0034203 glycolipid translocation [PMID:11807558]
Membrane protein required for translocation of Man5GlcNac2-PP-Dol; required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein; essential gene
DNA-(apurinic or apyrimidinic site) lyase APN2
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:10806210]
EXISTENCE:direct assay:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:10806210]
EXISTENCE:direct assay:GO:0008081 phosphoric diester hydrolase activity [PMID:11238902]
EXISTENCE:direct assay:GO:0008311 double-stranded DNA 3'-5' exodeoxyribonuclease activity [PMID:11238902]
EXISTENCE:direct assay:GO:0008408 3'-5' exonuclease activity [PMID:30778235]
EXISTENCE:genetic interaction:GO:0006284 base-excision repair [PMID:9765213]
Class II abasic (AP) endonuclease involved in repair of DNA damage; also possesses 3'-diesterase and PCNA-stiimulated 3' to 5' exonuclease activities; resolves blocked 3' ends that result from removal of misincorporated nucleotides; frays and cleaves 3' DNA termini facilitating 3' DNA-end healing via endonucleolytic cleavage; homolog of human APEX2 and E. coli exoIII
EXISTENCE:direct assay:GO:0000171 ribonuclease MRP activity [PMID:9620854]
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:9620854]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9620854]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9620854]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380]
Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:20936498]
EXISTENCE:mutant phenotype:GO:0000423 mitophagy [PMID:31332264]
EXISTENCE:mutant phenotype:GO:0000425 pexophagy [PMID:31332264]
EXISTENCE:mutant phenotype:GO:0005048 signal sequence binding [PMID:8187177]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:8187177]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:8187177]
Type I transmembrane sorting receptor for multiple vacuolar hydrolases; plays role in post-ER quality control in the Golgi, involved in protein targeting from the endoplasmic reticulum to the vacuole for degradation; cycles between late-Golgi and prevacuolar endosome-like compartments; targeted to vacuole via AP-3 pathway
mitogen-activated serine/threonine-protein kinase FUS3
EXISTENCE:direct assay:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:15620357|PMID:8384702]
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0004707 MAP kinase activity [PMID:15620357|PMID:8384702]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10233162|PMID:18417610]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10233162]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:11781566|PMID:17952059]
EXISTENCE:direct assay:GO:0071507 pheromone response MAPK cascade [PMID:8159759]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:10652102|PMID:15620357|PMID:8001818]
EXISTENCE:mutant phenotype:GO:0010526 negative regulation of transposition, RNA-mediated [PMID:9566871]
EXISTENCE:mutant phenotype:GO:0046827 positive regulation of protein export from nucleus [PMID:22588722]
EXISTENCE:physical interaction:GO:0043409 negative regulation of MAPK cascade [PMID:16424299]
Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
EXISTENCE:direct assay:GO:0003986 acetyl-CoA hydrolase activity [PMID:12606555]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:12606555|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:11746603]
EXISTENCE:direct assay:GO:0008775 acetate CoA-transferase activity [PMID:19298859]
EXISTENCE:mutant phenotype:GO:0006083 acetate metabolic process [PMID:8841387]
Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
Isoleucine tRNA (tRNA-Ile), predicted by tRNAscan-SE analysis
Glycine tRNA (tRNA-Gly), predicted by tRNAscan-SE analysis
ARS231; Autonomously Replicating Sequence
EXISTENCE:curator inference:GO:0001164 RNA polymerase I core promoter sequence-specific DNA binding []
EXISTENCE:direct assay:GO:0001163 RNA polymerase I transcription regulatory region sequence-specific DNA binding [PMID:20421203]
EXISTENCE:direct assay:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:11032814]
EXISTENCE:direct assay:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7958901]
EXISTENCE:direct assay:GO:0070860 RNA polymerase I core factor complex [PMID:7958901|PMID:8702872|PMID:8887672]
EXISTENCE:mutant phenotype:GO:0042790 nucleolar large rRNA transcription by RNA polymerase I [PMID:7958901]
EXISTENCE:physical interaction:GO:0001179 RNA polymerase I general transcription initiation factor binding [PMID:11032814]
Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
EXISTENCE:direct assay:GO:0004479 methionyl-tRNA formyltransferase activity [PMID:12549912]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:5277072|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0071951 conversion of methionyl-tRNA to N-formyl-methionyl-tRNA [PMID:12549912]
EXISTENCE:mutant phenotype:GO:0004479 methionyl-tRNA formyltransferase activity [PMID:10781559]
EXISTENCE:mutant phenotype:GO:0071951 conversion of methionyl-tRNA to N-formyl-methionyl-tRNA [PMID:10781559]
Methionyl-tRNA formyltransferase; catalyzes formylation of mitochondria initiator Met-tRNA and residual cytosolic Met-tRNA due to inefficient translocation into the mitochondrial matrix; starvation or stationary phase entry increases Gcn2p phosphorylation-mediated cytosolic retention of Fmt1p, enhancing N-terminal protein formylation and ultimately N-end rule pathway mediated destruction that involves the Psh1p ubiquitin ligase; potential Cdc28p substrate
bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1
EXISTENCE:direct assay:GO:0004366 glycerol-3-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762|PMID:19525420]
EXISTENCE:direct assay:GO:0016287 glycerone-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:mutant phenotype:GO:0004366 glycerol-3-phosphate O-acyltransferase activity [PMID:11544256]
EXISTENCE:mutant phenotype:GO:0008654 phospholipid biosynthetic process [PMID:11544256]
Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed
EXISTENCE:direct assay:GO:0030136 clathrin-coated vesicle [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:30523155]
EXISTENCE:mutant phenotype:GO:0035653 clathrin-coated vesicle cargo loading, AP-1-mediated [PMID:30523155]
Clathrin adaptor (adaptin) accessory protein; physically interacts with and is involved in localization of clathrin adaptor protein complex-1 (AP-1); green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16855400]
EXISTENCE:mutant phenotype:GO:0000278 mitotic cell cycle [PMID:16855400]
EXISTENCE:mutant phenotype:GO:0044879 mitotic morphogenesis checkpoint signaling [PMID:33585466]
Atypical protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of formins and polarisome components in mitosis, and survival after spindle assembly checkpoint delay; negatively regulates the morphogenesis checkpoint in response to polarity insults in opposition to ALK1; phosphorylated in response to DNA damage; similar to mammalian haspins
hypothetical protein; identified by fungal homology and RT-PCR
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:9001207]
EXISTENCE:direct assay:GO:0000417 HIR complex [PMID:16264190|PMID:16303565]
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:11782447]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:16264190]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9001207]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:16264190|PMID:16303565]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:16449659]
EXISTENCE:genetic interaction:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:23962978]
EXISTENCE:mutant phenotype:GO:0016480 negative regulation of transcription by RNA polymerase III [PMID:23962978]
EXISTENCE:mutant phenotype:GO:0031491 nucleosome binding [PMID:16264190]
EXISTENCE:physical interaction:GO:0000417 HIR complex [PMID:16264190]
Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
cytoskeletal protein-binding protein SLA1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:17286805]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11950888]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10198057|PMID:16824951]
EXISTENCE:direct assay:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:12814545|PMID:22973053]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:17244534]
EXISTENCE:direct assay:GO:0140224 SLAC complex [PMID:22973053]
EXISTENCE:direct assay:GO:1990964 actin cytoskeleton-regulatory complex [PMID:10594004]
EXISTENCE:mutant phenotype:GO:0000147 actin cortical patch assembly [PMID:10198057|PMID:8335689]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:17286805]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11940605|PMID:17363896]
EXISTENCE:mutant phenotype:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:12814545]
EXISTENCE:mutant phenotype:GO:0140312 cargo adaptor activity [PMID:11940605|PMID:17065552|PMID:17363896]
EXISTENCE:physical interaction:GO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation [PMID:22973053]
EXISTENCE:physical interaction:GO:0140224 SLAC complex [PMID:22973053]
Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
EXISTENCE:direct assay:GO:0006337 nucleosome disassembly [PMID:16492771]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:12183366]
EXISTENCE:direct assay:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:17081996]
EXISTENCE:direct assay:GO:0016586 RSC-type complex [PMID:10619019|PMID:12183366]
EXISTENCE:genetic interaction:GO:0016586 RSC-type complex [PMID:16204215]
EXISTENCE:mutant phenotype:GO:0016586 RSC-type complex [PMID:16204215]
EXISTENCE:physical interaction:GO:0016586 RSC-type complex [PMID:16204215]
Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
drug-responsive transcription factor PDR3
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:8798494]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11136452|PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276]
EXISTENCE:genetic interaction:GO:2001040 positive regulation of cellular response to drug [PMID:8078477]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8798494|PMID:9428726]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8798494|PMID:9428726]
EXISTENCE:mutant phenotype:GO:2001040 positive regulation of cellular response to drug [PMID:8078477|PMID:9767597]
Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate
YBLWTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:17652137]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:15590835|PMID:17652137|PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:17652137|PMID:14562095]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:17652137|PMID:12150911]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:17652137]
EXISTENCE:direct assay:GO:0032040 small-subunit processome [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835|PMID:17652137]
EXISTENCE:mutant phenotype:GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835|PMID:17652137]
EXISTENCE:mutant phenotype:GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:17652137|PMID:15590835]
Component of the small-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15781691]
Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
Non-essential hypothetical protein; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
ARS208; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0004555 alpha,alpha-trehalase activity [PMID:18065618]
EXISTENCE:mutant phenotype:GO:0005993 trehalose catabolic process [PMID:18065618]
Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
ditrans,polycis-polyprenyl diphosphate synthase
EXISTENCE:direct assay:GO:0004659 prenyltransferase activity [PMID:17345630|PMID:9858571]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:11442630|PMID:24868093|PMID:9858571|PMID:26928762]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24868093]
EXISTENCE:direct assay:GO:0006486 protein glycosylation [PMID:9858571]
EXISTENCE:direct assay:GO:0019408 dolichol biosynthetic process [PMID:10217761|PMID:25066056]
EXISTENCE:direct assay:GO:0045547 dehydrodolichyl diphosphate synthase activity [PMID:11442630|PMID:25066056]
EXISTENCE:direct assay:GO:1904423 dehydrodolichyl diphosphate synthase complex [PMID:25066056]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:9858571]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:12911815]
Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1; major enzyme of polyprenol synthesis in both the endoplasmic reticulum (ER) and in lipid droplets; participates in ER protein sorting; human ortholog DHDDS functionally complements the heat sensitive growth defect of a ts allele, and is associated with retinitis pigmentosa
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0000010 trans-hexaprenyltranstransferase activity [PMID:9708911]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:10760477]
EXISTENCE:mutant phenotype:GO:0006744 ubiquinone biosynthetic process [PMID:9708911]
Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17615295|PMID:26928762]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17615295]
EXISTENCE:genetic interaction:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:15720390]
EXISTENCE:genetic interaction:GO:0000030 mannosyltransferase activity [PMID:15623507]
EXISTENCE:genetic interaction:GO:0006506 GPI anchor biosynthetic process [PMID:15623507]
EXISTENCE:genetic interaction:GO:0031501 mannosyltransferase complex [PMID:17615295]
EXISTENCE:mutant phenotype:GO:0000009 alpha-1,6-mannosyltransferase activity [PMID:15720390]
EXISTENCE:mutant phenotype:GO:0000030 mannosyltransferase activity [PMID:15720390]
EXISTENCE:mutant phenotype:GO:0006506 GPI anchor biosynthetic process [PMID:15720390]
EXISTENCE:physical interaction:GO:0031501 mannosyltransferase complex [PMID:17615295]
Functional ortholog of human PIG-V; PIG-V is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:15590673]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:32421152]
EXISTENCE:direct assay:GO:0043495 protein-membrane adaptor activity [PMID:32421152]
EXISTENCE:direct assay:GO:0072659 protein localization to plasma membrane [PMID:32421152]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17287526]
EXISTENCE:mutant phenotype:GO:0006033 chitin localization [PMID:15590673]
Plasma membrane (PM) ubiquitin ligase-substrate adaptor; involved in cell wall chitin deposition; ubiquitinates and down-regulates chitin synthase Chs3p abundance at PM via calcineurin/Crz1 signaling pathway which regulates RCR1 expression; delivered to PM by exomer complex using novel PM sorting motif; RCR1 has a paralog, RCR2, that arose from the whole genome duplication
succinate-semialdehyde dehydrogenase (NAD(P)(+))
EXISTENCE:genetic interaction:GO:0006540 glutamate decarboxylation to succinate [PMID:11031268]
EXISTENCE:mutant phenotype:GO:0006540 glutamate decarboxylation to succinate [PMID:11031268]
EXISTENCE:mutant phenotype:GO:0009013 succinate-semialdehyde dehydrogenase [NAD(P)+] activity [PMID:11031268|PMID:3888627]
EXISTENCE:mutant phenotype:GO:0009450 gamma-aminobutyric acid catabolic process [PMID:11031268|PMID:3888627]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11031268]
Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:22842922]
Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10903515]
EXISTENCE:genetic interaction:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:10572257|PMID:9235926]
EXISTENCE:mutant phenotype:GO:0015244 fluconazole transmembrane transporter activity [PMID:9235926]
EXISTENCE:mutant phenotype:GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane [PMID:10572257]
Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
ARS209; Autonomously Replicating Sequence; originally referred to as H4 ARS
Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; involved in mRNA multiplexing; N-terminal domain involved in maintaining genomic integrity
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11914276]
EXISTENCE:direct assay:GO:0008823 cupric reductase activity [PMID:32631887]
EXISTENCE:direct assay:GO:0043505 CENP-A containing nucleosome [PMID:22693454]
EXISTENCE:mutant phenotype:GO:0006878 cellular copper ion homeostasis [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0008823 cupric reductase activity [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:32631887]
EXISTENCE:mutant phenotype:GO:0009303 rRNA transcription [PMID:16002464]
EXISTENCE:mutant phenotype:GO:0043935 sexual sporulation resulting in formation of a cellular spore [PMID:20713519]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:21460225]
Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
EXISTENCE:curator inference:GO:0006796 phosphate-containing compound metabolic process [PMID:8224193]
EXISTENCE:direct assay:GO:0004427 inorganic diphosphate phosphatase activity [PMID:8224193]
Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YBRWTy1-2; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains co-transcribed genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats
hypothetical protein; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum while mCherry fusion protein localizes to both the endoplasmic reticulum and vacuole
Threonine tRNA (tRNA-Thr), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095]
EXISTENCE:direct assay:GO:0004362 glutathione-disulfide reductase (NADPH) activity [PMID:18400945]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:18503006]
EXISTENCE:direct assay:GO:0005796 Golgi lumen [PMID:18400945]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:18400945]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:18503006]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:18400945]
Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:9756928]
EXISTENCE:mutant phenotype:GO:0000026 alpha-1,2-mannosyltransferase activity [PMID:9756928]
EXISTENCE:mutant phenotype:GO:0006486 protein glycosylation [PMID:9756928]
Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12514182]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:12514182]
EXISTENCE:direct assay:GO:0070250 mating projection membrane [PMID:12514182]
Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:8849456]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:19198597]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:19198597]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:19366694|PMID:8849456|PMID:9488461]
EXISTENCE:direct assay:GO:0008139 nuclear localization sequence binding [PMID:9488461]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:19366694|PMID:8849456]
EXISTENCE:mutant phenotype:GO:0010458 exit from mitosis [PMID:12524331]
Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis
UDP-glucose:hexose-1-phosphate uridylyltransferase
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10993714]
EXISTENCE:direct assay:GO:0008108 UDP-glucose:hexose-1-phosphate uridylyltransferase activity [PMID:387754]
EXISTENCE:direct assay:GO:0033499 galactose catabolic process via UDP-galactose [PMID:387754]
EXISTENCE:mutant phenotype:GO:0008108 UDP-glucose:hexose-1-phosphate uridylyltransferase activity [PMID:14158615]
EXISTENCE:mutant phenotype:GO:0033499 galactose catabolic process via UDP-galactose [PMID:14158615]
Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism; human homolog UGP2 can complement yeast null mutant
bifunctional UDP-glucose 4-epimerase/aldose 1-epimerase
EXISTENCE:direct assay:GO:0003978 UDP-glucose 4-epimerase activity [PMID:14764091]
EXISTENCE:direct assay:GO:0004034 aldose 1-epimerase activity [PMID:14764091]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:14764091]
EXISTENCE:direct assay:GO:0033499 galactose catabolic process via UDP-galactose [PMID:14764091]
EXISTENCE:mutant phenotype:GO:0003978 UDP-glucose 4-epimerase activity [PMID:14158615]
UDP-glucose-4-epimerase; catalyzes interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers; human homolog GALE implicated in galactosemia, can complement yeast null mutant
lncRNA antisense to GAL10 and overlapping GAL1 mRNAs; GAL10-ncRNA transcription recruits Set2p methyltransferase and histone deacetylation activities in cis, leading to stable changes in chromatin structure; acts to enhance glucose repression of GAL1-10 induction at low environmental sugar concentrations; expression driven by Reb1p; does not appear to control GAL1 induction
EXISTENCE:direct assay:GO:0004335 galactokinase activity [PMID:14144]
EXISTENCE:direct assay:GO:0033499 galactose catabolic process via UDP-galactose [PMID:14144]
EXISTENCE:direct assay:GO:0046835 carbohydrate phosphorylation [PMID:14144]
EXISTENCE:genetic interaction:GO:0000411 positive regulation of transcription by galactose [PMID:2199310]
EXISTENCE:genetic interaction:GO:0005737 cytoplasm [PMID:2199310]
EXISTENCE:mutant phenotype:GO:0004335 galactokinase activity [PMID:13495476]
EXISTENCE:mutant phenotype:GO:0033499 galactose catabolic process via UDP-galactose [PMID:13495476]
Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; human homolog GALK2 complements yeast null mutant; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:8948441]
EXISTENCE:direct assay:GO:0015505 uracil:cation symporter activity [PMID:8948441]
EXISTENCE:direct assay:GO:0015857 uracil transport [PMID:8948441]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:15536122]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:8948441]
Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
EXISTENCE:direct assay:GO:0016791 phosphatase activity [PMID:10550052|PMID:15684411]
EXISTENCE:direct assay:GO:0047407 ADP-ribosyl-[dinitrogen reductase] hydrolase activity [PMID:23474712]
Phosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9008706|PMID:15470103|PMID:8909536]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:15755916]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:9008706]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:15470103|PMID:8909536]
EXISTENCE:direct assay:GO:0045009 chitosome [PMID:8970154]
EXISTENCE:mutant phenotype:GO:0004100 chitin synthase activity [PMID:9760183]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:1293886]
EXISTENCE:mutant phenotype:GO:0034221 fungal-type cell wall chitin biosynthetic process [PMID:3280554]
EXISTENCE:mutant phenotype:GO:0097271 protein localization to bud neck [PMID:29405545]
Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:25959673]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:8702795]
EXISTENCE:genetic interaction:GO:0034599 cellular response to oxidative stress [PMID:30593977]
EXISTENCE:genetic interaction:GO:0045454 cell redox homeostasis [PMID:30593977]
Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10684247]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26359986]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:17430889]
EXISTENCE:mutant phenotype:GO:1900036 positive regulation of cellular response to heat [PMID:34571008]
P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; positively regulates heat shock response; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:11509667|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0016631 enoyl-[acyl-carrier-protein] reductase activity [PMID:11509667]
EXISTENCE:mutant phenotype:GO:0006633 fatty acid biosynthetic process [PMID:11509667]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:11509667]
2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant
hypothetical protein; conserved among S. cerevisiae strains; YBR027C is not an essential gene
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:26582391]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:14562095]
EXISTENCE:direct assay:GO:0018105 peptidyl-serine phosphorylation [PMID:26582391]
EXISTENCE:direct assay:GO:0046777 protein autophosphorylation [PMID:26582391]
EXISTENCE:genetic interaction:GO:0004674 protein serine/threonine kinase activity [PMID:26582391]
EXISTENCE:genetic interaction:GO:0018105 peptidyl-serine phosphorylation [PMID:26582391]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:26582391]
EXISTENCE:mutant phenotype:GO:0018105 peptidyl-serine phosphorylation [PMID:25767889|PMID:26582391]
EXISTENCE:mutant phenotype:GO:0046777 protein autophosphorylation [PMID:26582391]
AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
EXISTENCE:direct assay:GO:0004605 phosphatidate cytidylyltransferase activity [PMID:8557688]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:23623749|PMID:8557688|PMID:26928762]
EXISTENCE:direct assay:GO:0070319 Golgi to plasma membrane transport vesicle [PMID:2163397]
EXISTENCE:mutant phenotype:GO:0006658 phosphatidylserine metabolic process [PMID:2832385]
EXISTENCE:mutant phenotype:GO:0016024 CDP-diacylglycerol biosynthetic process [PMID:2832385]
EXISTENCE:mutant phenotype:GO:0046488 phosphatidylinositol metabolic process [PMID:2832385|PMID:8557688]
Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids; human homolog CDS1 can complement yeast cds1 null mutant
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476|PMID:14562095]
EXISTENCE:genetic interaction:GO:0016279 protein-lysine N-methyltransferase activity [PMID:18957409]
EXISTENCE:genetic interaction:GO:0018026 peptidyl-lysine monomethylation [PMID:18957409]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:18957409]
EXISTENCE:mutant phenotype:GO:0018026 peptidyl-lysine monomethylation [PMID:18957409|PMID:24517342]
Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia
Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication
hypothetical protein; conserved among S. cerevisiae strains; YBR032W is not an essential gene
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667]
Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication
protein-arginine omega-N methyltransferase HMT1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:8668183]
EXISTENCE:direct assay:GO:0018216 peptidyl-arginine methylation [PMID:22997150|PMID:25715670|PMID:8647869]
EXISTENCE:direct assay:GO:0035241 protein-arginine omega-N monomethyltransferase activity [PMID:8647869]
EXISTENCE:direct assay:GO:0035242 protein-arginine omega-N asymmetric methyltransferase activity [PMID:8647869]
EXISTENCE:mutant phenotype:GO:0006406 mRNA export from nucleus [PMID:8668183]
EXISTENCE:mutant phenotype:GO:0018216 peptidyl-arginine methylation [PMID:18515076|PMID:20035717|PMID:8647869]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:20053728]
EXISTENCE:mutant phenotype:GO:0035241 protein-arginine omega-N monomethyltransferase activity [PMID:8647869]
EXISTENCE:mutant phenotype:GO:0035242 protein-arginine omega-N asymmetric methyltransferase activity [PMID:8647869]
EXISTENCE:mutant phenotype:GO:0046656 folic acid biosynthetic process [PMID:31515157]
EXISTENCE:mutant phenotype:GO:0060567 negative regulation of termination of DNA-templated transcription [PMID:20053728]
Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p, ribosomal protein Rps2p, and histones H3 and H4; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region
EXISTENCE:curator inference:GO:0009443 pyridoxal 5'-phosphate salvage [PMID:7896706]
EXISTENCE:direct assay:GO:0004733 pyridoxamine-phosphate oxidase activity [PMID:26327315]
EXISTENCE:direct assay:GO:0010181 FMN binding [PMID:26327315]
EXISTENCE:mutant phenotype:GO:0004733 pyridoxamine-phosphate oxidase activity [PMID:7896706]
Pyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:15306656]
EXISTENCE:mutant phenotype:GO:0030559 rRNA pseudouridylation guide activity [PMID:15306656|PMID:15923376]
EXISTENCE:mutant phenotype:GO:0031118 rRNA pseudouridine synthesis [PMID:15306656|PMID:15923376]
EXISTENCE:physical interaction:GO:0031429 box H/ACA snoRNP complex [PMID:15923376]
H/ACA small nucleolar RNA (snoRNA); guides pseudouridylation of small subunit (SSU) rRNA at positions U632 and U766
EXISTENCE:direct assay:GO:0000333 telomerase catalytic core complex [PMID:15813705]
EXISTENCE:direct assay:GO:0003720 telomerase activity [PMID:15813705]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12101098]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:10898986]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12101098]
EXISTENCE:direct assay:GO:0007004 telomere maintenance via telomerase [PMID:15813705]
EXISTENCE:mutant phenotype:GO:0000722 telomere maintenance via recombination [PMID:23390378|PMID:25822194]
EXISTENCE:mutant phenotype:GO:0007004 telomere maintenance via telomerase [PMID:15813705]
RNA template component of telomerase; flexible scaffold that tethers telomerase holoenzyme protein subunits to the complex; contains template sequence that Est2p uses to add irregular repeats of TG(1-3) residues onto a DNA end; promoter regulated by the cell cycle dependent transcriptional activators Swi4p/Swi6p and Mbp1p/Swi6p
mannosylinositol phosphorylceramide synthase regulatory subunit
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:7854312]
EXISTENCE:mutant phenotype:GO:0006688 glycosphingolipid biosynthetic process [PMID:8063782]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:7854312]
EXISTENCE:mutant phenotype:GO:0030234 enzyme regulator activity [PMID:12954640]
Endoplasmic reticulum membrane protein; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005507 copper ion binding [PMID:11546815]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:1944230]
EXISTENCE:mutant phenotype:GO:0033617 mitochondrial cytochrome c oxidase assembly [PMID:10854440]
Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004100 chitin synthase activity [PMID:9990311]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:8909536|PMID:15772160]
EXISTENCE:mutant phenotype:GO:0006031 chitin biosynthetic process [PMID:22573892]
EXISTENCE:mutant phenotype:GO:1902406 mitotic actomyosin contractile ring maintenance [PMID:15772160]
Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
EXISTENCE:direct assay:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:17082766]
EXISTENCE:mutant phenotype:GO:0005756 mitochondrial proton-transporting ATP synthase, central stalk [PMID:7929329]
EXISTENCE:mutant phenotype:GO:0015986 proton motive force-driven ATP synthesis [PMID:7929329]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:7929329]
Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9456310]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12374868]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:9456310]
EXISTENCE:mutant phenotype:GO:0000755 cytogamy [PMID:9456310]
Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating; targeted to vacuole via AP-3 pathway
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9988704]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:10515935|PMID:24868093]
EXISTENCE:direct assay:GO:0009898 cytoplasmic side of plasma membrane [PMID:17679730]
EXISTENCE:genetic interaction:GO:0044539 long-chain fatty acid import into cell [PMID:12601005]
EXISTENCE:mutant phenotype:GO:0000038 very long-chain fatty acid metabolic process [PMID:9660783|PMID:9988704]
EXISTENCE:mutant phenotype:GO:0005324 long-chain fatty acid transporter activity [PMID:9079682]
EXISTENCE:mutant phenotype:GO:0005777 peroxisome [PMID:9660783]
EXISTENCE:mutant phenotype:GO:0006515 protein quality control for misfolded or incompletely synthesized proteins [PMID:31746669]
EXISTENCE:mutant phenotype:GO:0031957 very long-chain fatty acid-CoA ligase activity [PMID:9660783]
EXISTENCE:mutant phenotype:GO:0044539 long-chain fatty acid import into cell [PMID:10880966|PMID:12601005]
Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:14562095]
EXISTENCE:mutant phenotype:GO:0016746 acyltransferase activity [PMID:19796168|PMID:27462707]
EXISTENCE:mutant phenotype:GO:0036149 phosphatidylinositol acyl-chain remodeling [PMID:19796168|PMID:27462707]
Acyltransferase; enzyme mainly responsible for the introduction of saturated very long chain fatty acids into neo-synthesized molecules of phosphatidylinositol; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:15649438|PMID:34887841]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
EXISTENCE:mutant phenotype:GO:0010509 polyamine homeostasis [PMID:21148207]
EXISTENCE:mutant phenotype:GO:0015203 polyamine transmembrane transporter activity [PMID:21148207]
EXISTENCE:mutant phenotype:GO:0015562 efflux transmembrane transporter activity [PMID:15649438]
EXISTENCE:mutant phenotype:GO:0015565 threonine efflux transmembrane transporter activity [PMID:34887841]
EXISTENCE:mutant phenotype:GO:0032973 amino acid export across plasma membrane [PMID:34887841]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15649438]
Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:9822678]
EXISTENCE:mutant phenotype:GO:0034553 mitochondrial respiratory chain complex II assembly [PMID:9822678]
Protein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone
ARS211; Autonomously Replicating Sequence
Valine tRNA (tRNA-Val), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:11724821]
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:11724821]
EXISTENCE:mutant phenotype:GO:0008157 protein phosphatase 1 binding [PMID:9584086]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:11724821]
EXISTENCE:mutant phenotype:GO:0043666 regulation of phosphoprotein phosphatase activity [PMID:11724821]
EXISTENCE:physical interaction:GO:0000164 protein phosphatase type 1 complex [PMID:9584086]
Meiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p
EXISTENCE:direct assay:GO:0003960 NADPH:quinone reductase activity [PMID:17497241]
EXISTENCE:direct assay:GO:0032440 2-alkenal reductase [NAD(P)+] activity [PMID:21276778]
EXISTENCE:direct assay:GO:0035925 mRNA 3'-UTR AU-rich region binding [PMID:17497241|PMID:19007762]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:17497241]
NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:14576278|PMID:16823961]
hypothetical protein; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:curator inference:GO:0002181 cytoplasmic translation [PMID:6814480]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:6814480]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:6814480]
EXISTENCE:mutant phenotype:GO:0000028 ribosomal small subunit assembly [PMID:8070651]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:27601258]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:20852627]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:25479637|PMID:27601258]
EXISTENCE:mutant phenotype:GO:0001174 transcriptional start site selection at RNA polymerase II promoter [PMID:27601258]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:27601258]
EXISTENCE:mutant phenotype:GO:0006369 termination of RNA polymerase II transcription [PMID:25479637]
RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:8649403]
EXISTENCE:mutant phenotype:GO:0043666 regulation of phosphoprotein phosphatase activity [PMID:8649403]
EXISTENCE:physical interaction:GO:0000164 protein phosphatase type 1 complex [PMID:8649403]
Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21442317|PMID:14562095]
EXISTENCE:direct assay:GO:0032126 eisosome [PMID:19064668]
hypothetical protein; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
hypothetical protein; induced by cell wall perturbation
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:25503505]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:14562095]
Protein with a putative role in response to acid stress; null mutant is sensitive to acetic acid; transcription is regulated by Haa1p and induced in the presence of acetic acid; protein observed in plasma membrane foci in the presence of acetic acid; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:10377396|PMID:10449419|PMID:8479905]
EXISTENCE:direct assay:GO:0071001 U4/U6 snRNP [PMID:8479905]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:2147224]
EXISTENCE:mutant phenotype:GO:0071001 U4/U6 snRNP [PMID:2147224]
Splicing factor; component of the U4/U6-U5 snRNP complex
17-beta-hydroxysteroid dehydrogenase-like protein
Putative glycoside hydrolase of the mitochondrial intermembrane space
Glutamine tRNA (tRNA-Gln), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
Putative membrane protein upregulated in toxic manganese levels; mRNA identified as translated by ribosome profiling data; partially overlaps dubious ORF YBR056C-B; YBR056W-A has a paralog, YDR034W-B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0036396 RNA N6-methyladenosine methyltransferase complex [PMID:22685417]
EXISTENCE:genetic interaction:GO:0080009 mRNA methylation [PMID:22685417]
EXISTENCE:mutant phenotype:GO:0006279 premeiotic DNA replication [PMID:9504908]
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:22685417]
EXISTENCE:mutant phenotype:GO:0080009 mRNA methylation [PMID:22685417]
EXISTENCE:mutant phenotype:GO:2000221 negative regulation of pseudohyphal growth [PMID:22685417]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:22685417]
EXISTENCE:physical interaction:GO:0036396 RNA N6-methyladenosine methyltransferase complex [PMID:22685417]
Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:8982460]
EXISTENCE:direct assay:GO:0004843 cysteine-type deubiquitinase activity [PMID:19410548]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616]
EXISTENCE:mutant phenotype:GO:0045721 negative regulation of gluconeogenesis [PMID:12686616]
Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:10713067]
EXISTENCE:direct assay:GO:0035339 SPOTS complex [PMID:20182505]
EXISTENCE:mutant phenotype:GO:0008047 enzyme activator activity [PMID:10713067]
EXISTENCE:mutant phenotype:GO:0090154 positive regulation of sphingolipid biosynthetic process [PMID:10713067|PMID:23286903]
EXISTENCE:physical interaction:GO:0006666 3-keto-sphinganine metabolic process [PMID:10713067]
Protein that stimulates the activity of serine palmitoyltransferase; involved in sphingolipid biosynthesis; Lcb1p and Lcb2p are the two components of serine palmitoyltransferase
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:29895620|PMID:12956961]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:24390141]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:14562095|PMID:26928762]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:29895620]
EXISTENCE:mutant phenotype:GO:1900186 negative regulation of clathrin-dependent endocytosis [PMID:29895620|PMID:28069741|PMID:17178891]
Ser/Thr protein kinase; phosphorylates Pan1p, Sla1p and Ent1p to negatively regulate endocytosis in response to membrane stress; regulates actin cytoskeleton organization and clathrin-dependent endocytosis; phosphorylated and inhibited by upstream kinase, Fpk1p; member, along with Ark1p and Prk1p, of the Ark kinase family
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:9159120|PMID:9288745]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:16824194|PMID:9288745]
EXISTENCE:direct assay:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:direct assay:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:12897051]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:7579692]
EXISTENCE:mutant phenotype:GO:0005664 nuclear origin of replication recognition complex [PMID:9372948]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:16716188|PMID:7579692]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16980387]
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
EXISTENCE:direct assay:GO:0002128 tRNA nucleoside ribose methylation [PMID:11927565]
EXISTENCE:direct assay:GO:0002130 wobble position ribose methylation [PMID:11927565]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11927565]
EXISTENCE:direct assay:GO:0106050 tRNA 2'-O-methyltransferase activity [PMID:11927565]
EXISTENCE:mutant phenotype:GO:0002130 wobble position ribose methylation [PMID:22912484]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:11927565]
2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively; yeast null mutant can be functionally complemented by human FTSJ1, mutations in which have been implicated in nonsyndromic X-linked intellectual disability (NSXLID)
hypothetical protein that interacts with Msb2p; may play a role in activation of the filamentous growth pathway
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:34694322]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:34694322]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:34694322]
EXISTENCE:direct assay:GO:0044232 organelle membrane contact site [PMID:34694322]
EXISTENCE:direct assay:GO:0140056 organelle localization by membrane tethering [PMID:34694322]
Molecular tether on nuclear membrane; mediates contact by interacting with Tom70p on mitochondria; protein abundance is regulated by phosphatidylcholine, enabling coupling of phospholipid homeostasis with contact extent
EXISTENCE:direct assay:GO:0017070 U6 snRNA binding [PMID:33547186]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:9528778]
Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:20802496]
EXISTENCE:genetic interaction:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:12024013|PMID:15466424|PMID:16278455]
EXISTENCE:genetic interaction:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:16278455]
EXISTENCE:genetic interaction:GO:0043709 cell adhesion involved in single-species biofilm formation [PMID:12024013]
EXISTENCE:genetic interaction:GO:2000221 negative regulation of pseudohyphal growth [PMID:12024013]
EXISTENCE:mutant phenotype:GO:0000433 carbon catabolite repression of transcription from RNA polymerase II promoter by glucose [PMID:11404322]
Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose
EXISTENCE:direct assay:GO:0005199 structural constituent of cell wall [PMID:10216230]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:10216230|PMID:15781460]
EXISTENCE:direct assay:GO:0016298 lipase activity [PMID:9818717]
EXISTENCE:direct assay:GO:0031505 fungal-type cell wall organization [PMID:10216230]
Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10654085]
EXISTENCE:direct assay:GO:0006865 amino acid transport [PMID:10654085]
EXISTENCE:direct assay:GO:0015171 amino acid transmembrane transporter activity [PMID:10654085]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10654085]
High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:23666621]
EXISTENCE:genetic interaction:GO:0015827 tryptophan transport [PMID:7523855]
EXISTENCE:genetic interaction:GO:0022893 low-affinity tryptophan transmembrane transporter activity [PMID:7523855]
EXISTENCE:mutant phenotype:GO:0015828 tyrosine transport [PMID:7523855]
Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
Antisense lncRNA XUT_2F-154 (TBRT); transcription mediates repression of BAP2 and TAT1
N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14
EXISTENCE:direct assay:GO:0004577 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [PMID:16100113]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:35563734]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:16100110|PMID:17686769]
EXISTENCE:direct assay:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:16100113]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:15282802]
EXISTENCE:mutant phenotype:GO:0004577 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [PMID:15615718|PMID:16100113]
EXISTENCE:mutant phenotype:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:15615718|PMID:16100113]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:16100110|PMID:16100113]
EXISTENCE:physical interaction:GO:0006488 dolichol-linked oligosaccharide biosynthetic process [PMID:16100110]
EXISTENCE:physical interaction:GO:0043541 UDP-N-acetylglucosamine transferase complex [PMID:16100110|PMID:16100113]
Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; localizes to the peroxisome; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant; mutations of human ALG14 cause congenital myasthenic syndrome
ARS212; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21777356|PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:21777356]
hypothetical protein found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:20844764]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2645298]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:2645298|PMID:11914276]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:10581247]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405|PMID:24291094]
EXISTENCE:direct assay:GO:0034605 cellular response to heat [PMID:10581247]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:10581247]
Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
hypothetical protein; identified by fungal homology and RT-PCR; localizes to cytosol and peroxisomes
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:16831867]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:16831867]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:10970884]
EXISTENCE:direct assay:GO:0015616 DNA translocase activity [PMID:10970884]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:10970884]
EXISTENCE:direct assay:GO:0032392 DNA geometric change [PMID:10970884|PMID:16831867]
EXISTENCE:direct assay:GO:0032508 DNA duplex unwinding [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0003690 double-stranded DNA binding [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:9409819|PMID:9409820]
EXISTENCE:mutant phenotype:GO:0008094 ATP-dependent activity, acting on DNA [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0032392 DNA geometric change [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0032508 DNA duplex unwinding [PMID:16831867]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:16230461]
DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA, promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis, meiosis; implicated in cell divisions in telomerase-negative strains through adaptation to DNA damage, which allows bypass of cell cycle arrest and contributes senescence-specific genome instability; phosphorylated in response to DNA damage
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23679341|PMID:19001347|PMID:26928762]
Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to M28 family of metalloproteases; has lumenal-facing protease domain; proposed role in vacuole physiology; targeted to vacuole via AP-3 pathway
ARS213; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15989961|PMID:23708375|PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0031902 late endosome membrane [PMID:16732272]
EXISTENCE:direct assay:GO:0071986 Ragulator complex [PMID:16732272]
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:15539461]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:15989961]
EXISTENCE:physical interaction:GO:0071986 Ragulator complex [PMID:15989961]
Subunit of EGO/GSE complex; vacuolar/endosomal membrane-associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from endosome to plasma membrane; essential for integrity and function of EGO; targeted to vacuole via AP-3 pathway; gene exhibits synthetic genetic interaction with MSS4
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239|PMID:14576278]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12583915|PMID:16622836]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:15781460]
EXISTENCE:mutant phenotype:GO:0031505 fungal-type cell wall organization [PMID:15583168]
GPI-anchored protein involved in efficient glucose uptake; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication
translation initiation factor eIF3 core subunit a
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11302750]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:9671501]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:22842922]
EXISTENCE:direct assay:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9671501]
EXISTENCE:direct assay:GO:0006413 translational initiation [PMID:9671501]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:19470581|PMID:26777405]
EXISTENCE:direct assay:GO:0043614 multi-eIF complex [PMID:15838098]
EXISTENCE:mutant phenotype:GO:0002188 translation reinitiation [PMID:20679478]
EXISTENCE:physical interaction:GO:0005852 eukaryotic translation initiation factor 3 complex [PMID:9660829]
eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; essential for loading mRNA onto ribosome at entry and exit channels of preinitiation complex; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
EXISTENCE:direct assay:GO:0000149 SNARE binding [PMID:27364524]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:23613772]
EXISTENCE:direct assay:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9382859]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:10387016]
EXISTENCE:direct assay:GO:0048280 vesicle fusion with Golgi apparatus [PMID:9382859]
EXISTENCE:direct assay:GO:0070300 phosphatidic acid binding [PMID:27364524]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:11694599]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11694599]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:2071670]
EXISTENCE:mutant phenotype:GO:0048219 inter-Golgi cisterna vesicle-mediated transport [PMID:10637300|PMID:2071670]
AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
SAGA histone acetyltransferase complex subunit SPT7
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9224714|PMID:9674426]
EXISTENCE:direct assay:GO:0005198 structural molecule activity [PMID:12101232]
EXISTENCE:direct assay:GO:0006325 chromatin organization [PMID:9674426]
EXISTENCE:direct assay:GO:0046695 SLIK (SAGA-like) complex [PMID:12446794]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:7713415]
EXISTENCE:mutant phenotype:GO:0005198 structural molecule activity [PMID:9858534]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:12101232|PMID:9858534]
Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
Arginine tRNA (tRNA-Arg), predicted by tRNAscan-SE analysis; one of 11 nuclear tRNA genes containing the tDNA-anticodon UCU (converted to mcm5-UCU in the mature tRNA); decodes AGA codons into arginine; one of 19 nuclear tRNAs for arginine; part of dicistronic transcript with tD(GUC)B
Aspartate tRNA (tRNA-Asp), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:2154373|PMID:24034674]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:2154373]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:20598539]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20598539]
EXISTENCE:direct assay:GO:0006513 protein monoubiquitination [PMID:8391479]
EXISTENCE:direct assay:GO:0016567 protein ubiquitination [PMID:16926149]
EXISTENCE:direct assay:GO:0030674 protein-macromolecule adaptor activity [PMID:11788821]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21357418]
EXISTENCE:direct assay:GO:0070628 proteasome binding [PMID:10848595]
EXISTENCE:mutant phenotype:GO:0000209 protein polyubiquitination [PMID:32009536]
EXISTENCE:mutant phenotype:GO:0043130 ubiquitin binding [PMID:21357418]
EXISTENCE:mutant phenotype:GO:0043162 ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [PMID:11788821]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:20462952]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579]
Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
ARS214; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:20118212]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:9036858]
EXISTENCE:direct assay:GO:0003700 DNA-binding transcription factor activity [PMID:10652102]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19218425]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:10652102]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:9036858]
EXISTENCE:direct assay:GO:1990527 Tec1p-Ste12p-Dig1p complex [PMID:16782869]
EXISTENCE:direct assay:GO:2000222 positive regulation of pseudohyphal growth [PMID:9036858]
EXISTENCE:genetic interaction:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:16782869]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:16782869]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12455687]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:8730867|PMID:9036858|PMID:9055077]
EXISTENCE:mutant phenotype:GO:0010527 positive regulation of transposition, RNA-mediated [PMID:10652102]
Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1
EXISTENCE:direct assay:GO:0004329 formate-tetrahydrofolate ligase activity [PMID:3528153]
EXISTENCE:direct assay:GO:0004477 methenyltetrahydrofolate cyclohydrolase activity [PMID:3528153]
EXISTENCE:direct assay:GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity [PMID:3528153]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:3528153|PMID:16823961|PMID:14576278|PMID:24769239]
EXISTENCE:direct assay:GO:0046656 folic acid biosynthetic process [PMID:3528153]
EXISTENCE:mutant phenotype:GO:0006139 nucleobase-containing compound metabolic process [PMID:10781559]
Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
EXISTENCE:genetic interaction:GO:0009061 anaerobic respiration [PMID:1915842|PMID:2165073]
Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
ARS215; Autonomously Replicating Sequence; replication origin of very weak function
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:11030653|PMID:17234190]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:19453974]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:23237950]
EXISTENCE:direct assay:GO:0033101 cellular bud membrane [PMID:11030653|PMID:13679573]
EXISTENCE:mutant phenotype:GO:0072659 protein localization to plasma membrane [PMID:24416406]
Cortical ER protein involved in ER-plasma membrane (PM) tethering; one of 6 proteins (Ist2, Scs2, Scs22, Tcb1, Tcb2, Tcb3) that connect ER to PM, regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1 phosphatase to PI4P; required to locate Osh6, Osh7 in ER/PM contact site to allow phosphatidylserine transport from ER to PM; localizes to mother in small-budded cells, to bud in medium-, large-budded cells; mRNA transported to bud tip by actomyosin-driven process
EXISTENCE:direct assay:GO:0003689 DNA clamp loader activity [PMID:12604797|PMID:8995429]
EXISTENCE:direct assay:GO:0005663 DNA replication factor C complex [PMID:7651383]
EXISTENCE:direct assay:GO:0006272 leading strand elongation [PMID:1346062]
EXISTENCE:direct assay:GO:0031389 Rad17 RFC-like complex [PMID:12604797]
EXISTENCE:mutant phenotype:GO:0005663 DNA replication factor C complex [PMID:8559655]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:12665596]
EXISTENCE:physical interaction:GO:0031389 Rad17 RFC-like complex [PMID:10660302]
EXISTENCE:physical interaction:GO:0031390 Ctf18 RFC-like complex [PMID:11389843]
EXISTENCE:physical interaction:GO:0031391 Elg1 RFC-like complex [PMID:12909721|PMID:12912927]
Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15282802]
EXISTENCE:direct assay:GO:0005657 replication fork [PMID:12015307]
EXISTENCE:direct assay:GO:0006272 leading strand elongation [PMID:1682321]
EXISTENCE:direct assay:GO:0006273 lagging strand elongation [PMID:7673186]
EXISTENCE:direct assay:GO:0030337 DNA polymerase processivity factor activity [PMID:18635534|PMID:2902631]
EXISTENCE:direct assay:GO:1902394 positive regulation of exodeoxyribonuclease activity [PMID:12192046]
EXISTENCE:direct assay:GO:1903022 positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands [PMID:12192046]
EXISTENCE:direct assay:GO:1905779 positive regulation of exonuclease activity [PMID:30778235]
EXISTENCE:genetic interaction:GO:0000278 mitotic cell cycle [PMID:7516465]
EXISTENCE:genetic interaction:GO:0000710 meiotic mismatch repair [PMID:18245822]
EXISTENCE:genetic interaction:GO:0006298 mismatch repair [PMID:8910404|PMID:9504910]
EXISTENCE:genetic interaction:GO:0006301 postreplication repair [PMID:8790390]
EXISTENCE:genetic interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:16934511]
EXISTENCE:genetic interaction:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:10545450]
EXISTENCE:genetic interaction:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:16934511]
EXISTENCE:genetic interaction:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:16079237]
EXISTENCE:genetic interaction:GO:0070987 error-free translesion synthesis [PMID:19264809]
EXISTENCE:mutant phenotype:GO:0000710 meiotic mismatch repair [PMID:18245822]
EXISTENCE:mutant phenotype:GO:0006289 nucleotide-excision repair [PMID:9806417]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:8858149]
EXISTENCE:mutant phenotype:GO:0006301 postreplication repair [PMID:8790390]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:16157874]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:16157874]
EXISTENCE:mutant phenotype:GO:0035753 maintenance of DNA trinucleotide repeats [PMID:16079237]
EXISTENCE:physical interaction:GO:0006273 lagging strand elongation [PMID:7673186]
EXISTENCE:physical interaction:GO:0006298 mismatch repair [PMID:8858149]
EXISTENCE:physical interaction:GO:0007064 mitotic sister chromatid cohesion [PMID:16934511]
EXISTENCE:physical interaction:GO:0043626 PCNA complex [PMID:8001157]
Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays a crucial role during Okazaki fragment processing; leucine at position 164 is essential for stable complex formation with replicative DNA polymerase delta
high-mobility group nucleosome-binding protein
EXISTENCE:direct assay:GO:0001195 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III [PMID:16407207]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:3169249]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:7721780]
EXISTENCE:direct assay:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:17178828]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:14739928]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:8946917]
EXISTENCE:genetic interaction:GO:0070898 RNA polymerase III preinitiation complex assembly [PMID:11287614]
High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:18387953]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9430585|PMID:9495346]
EXISTENCE:direct assay:GO:0042721 TIM22 mitochondrial import inner membrane insertion complex [PMID:9495346]
EXISTENCE:direct assay:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9495346]
EXISTENCE:mutant phenotype:GO:0005543 phospholipid binding [PMID:18387953]
EXISTENCE:mutant phenotype:GO:0008320 protein transmembrane transporter activity [PMID:9430585|PMID:9495346]
EXISTENCE:mutant phenotype:GO:0045039 protein insertion into mitochondrial inner membrane [PMID:9430585|PMID:9495346]
Essential protein of the inner mitochondrial membrane; peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
EXISTENCE:direct assay:GO:0006796 phosphate-containing compound metabolic process [PMID:2407294]
EXISTENCE:direct assay:GO:0042723 thiamine-containing compound metabolic process [PMID:2407294]
EXISTENCE:mutant phenotype:GO:0003993 acid phosphatase activity [PMID:2676709]
EXISTENCE:mutant phenotype:GO:0030287 cell wall-bounded periplasmic space [PMID:2676709]
Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
EXISTENCE:direct assay:GO:0003993 acid phosphatase activity [PMID:8817921]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:3537693]
EXISTENCE:genetic interaction:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:genetic interaction:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0006796 phosphate-containing compound metabolic process [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0008361 regulation of cell size [PMID:12089449]
EXISTENCE:mutant phenotype:GO:0016036 cellular response to phosphate starvation [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0017111 ribonucleoside triphosphate phosphatase activity [PMID:16278456]
EXISTENCE:mutant phenotype:GO:0047429 nucleoside triphosphate diphosphatase activity [PMID:16278456]
Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
EXISTENCE:direct assay:GO:0000932 P-body [PMID:17307817]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
Putative tubulin tyrosine ligase associated with P-bodies; may have a role in mRNA metabolism; yeast knockout collection strain identified as a pby1 null mutant is actually wild-type for PBY1 and deleted for mms4
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16275642]
EXISTENCE:mutant phenotype:GO:0000747 conjugation with cellular fusion [PMID:10628851]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:10628851]
EXISTENCE:mutant phenotype:GO:0004407 histone deacetylase activity [PMID:16275642]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:2000219 positive regulation of invasive growth in response to glucose limitation [PMID:18202364]
Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the ER
ubiquitin-binding serine/threonine protein kinase VPS15
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:7989323]
EXISTENCE:direct assay:GO:0034271 phosphatidylinositol 3-kinase complex, class III, type I [PMID:11157979]
EXISTENCE:direct assay:GO:0034272 phosphatidylinositol 3-kinase complex, class III, type II [PMID:11157979]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:21070969]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:23335340]
EXISTENCE:mutant phenotype:GO:0000011 vacuole inheritance [PMID:1493335]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:7721937]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:11157979|PMID:32788210]
EXISTENCE:mutant phenotype:GO:0030242 autophagy of peroxisome [PMID:21121900]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:23335340]
EXISTENCE:mutant phenotype:GO:0045053 protein retention in Golgi apparatus [PMID:8649377]
EXISTENCE:mutant phenotype:GO:0045324 late endosome to vacuole transport [PMID:11157979]
EXISTENCE:mutant phenotype:GO:0048017 inositol lipid-mediated signaling [PMID:16839886]
Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
EXISTENCE:direct assay:GO:0004520 endodeoxyribonuclease activity [PMID:11641278]
EXISTENCE:direct assay:GO:0008821 crossover junction endodeoxyribonuclease activity [PMID:17363897]
EXISTENCE:direct assay:GO:0048476 Holliday junction resolvase complex [PMID:17363897]
EXISTENCE:genetic interaction:GO:0000712 resolution of meiotic recombination intermediates [PMID:18691964]
EXISTENCE:genetic interaction:GO:0006265 DNA topological change [PMID:11139495]
EXISTENCE:genetic interaction:GO:0007131 reciprocal meiotic recombination [PMID:11641278]
EXISTENCE:genetic interaction:GO:0010520 regulation of reciprocal meiotic recombination [PMID:18691965]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:9604884]
EXISTENCE:mutant phenotype:GO:0006974 cellular response to DNA damage stimulus [PMID:11139495]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:18615017]
Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:12052876]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:12052876]
EXISTENCE:direct assay:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:23530227]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:23530227]
Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for degradation; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
EXISTENCE:curator inference:GO:0090522 vesicle tethering involved in exocytosis [PMID:10438536]
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10438536]
EXISTENCE:direct assay:GO:0000145 exocyst [PMID:10438536]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10438536]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10438536]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:10438536]
EXISTENCE:mutant phenotype:GO:0000245 spliceosomal complex assembly [PMID:11425851]
EXISTENCE:mutant phenotype:GO:0001927 exocyst assembly [PMID:15788396]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:10438536|PMID:15788396]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:10438536|PMID:15788396]
EXISTENCE:mutant phenotype:GO:0051601 exocyst localization [PMID:15788396]
EXISTENCE:physical interaction:GO:0000245 spliceosomal complex assembly [PMID:11425851]
Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9651685]
EXISTENCE:direct assay:GO:0034967 Set3 complex [PMID:11711434]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:19379692]
EXISTENCE:mutant phenotype:GO:0032874 positive regulation of stress-activated MAPK cascade [PMID:18487345]
EXISTENCE:physical interaction:GO:0045835 negative regulation of meiotic nuclear division [PMID:11711434]
WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16140254|PMID:16823961|PMID:24769239]
EXISTENCE:genetic interaction:GO:0005342 organic acid transmembrane transporter activity [PMID:16140254]
EXISTENCE:genetic interaction:GO:0006839 mitochondrial transport [PMID:16140254]
EXISTENCE:genetic interaction:GO:0055085 transmembrane transport [PMID:16140254]
Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
glucose-induced degradation complex subunit VID24
EXISTENCE:direct assay:GO:0031410 cytoplasmic vesicle [PMID:9508768]
EXISTENCE:direct assay:GO:0034657 GID complex [PMID:16872538|PMID:18508925]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:9508768|PMID:12730205]
EXISTENCE:mutant phenotype:GO:0007039 protein catabolic process in the vacuole [PMID:15358789|PMID:9508768]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:12686616|PMID:15358789]
GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:27905431|PMID:26928762]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:8709965]
EXISTENCE:mutant phenotype:GO:0045047 protein targeting to ER [PMID:27905431]
EXISTENCE:mutant phenotype:GO:0051604 protein maturation [PMID:19433630]
Involved in SRP-independent protein targeting to the ER; component of an alternative ER-targeting pathway partially redundant with the GET pathway that can compensate for SRP loss; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; role in establishing nucleus-vacuole junctions (NVJs) in high glucose; targeted to the NVJ during glucose starvation to facilitate expansion; role in phosphate transport with Pho86p
EXISTENCE:direct assay:GO:0000940 outer kinetochore [PMID:12589047]
EXISTENCE:genetic interaction:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:15241623]
EXISTENCE:mutant phenotype:GO:0034087 establishment of mitotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0034089 establishment of meiotic sister chromatid cohesion [PMID:19730685]
EXISTENCE:mutant phenotype:GO:0034090 maintenance of meiotic sister chromatid cohesion [PMID:14752166]
EXISTENCE:mutant phenotype:GO:0045144 meiotic sister chromatid segregation [PMID:15241623]
EXISTENCE:mutant phenotype:GO:0071459 protein localization to chromosome, centromeric region [PMID:16357219|PMID:26653857]
Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:21035341]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:15561700]
EXISTENCE:genetic interaction:GO:0000147 actin cortical patch assembly [PMID:21035341]
EXISTENCE:mutant phenotype:GO:0051016 barbed-end actin filament capping [PMID:23333351]
Protein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:1639847]
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:3533275|PMID:8461293]
EXISTENCE:direct assay:GO:0005823 central plaque of spindle pole body [PMID:8601600|PMID:8886974]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:1639847]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:1639847|PMID:26928762]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:9859992]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:1639847]
EXISTENCE:direct assay:GO:0048306 calcium-dependent protein binding [PMID:1321337]
EXISTENCE:direct assay:GO:1903525 regulation of membrane tubulation [PMID:27093085]
EXISTENCE:genetic interaction:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12836012]
EXISTENCE:genetic interaction:GO:0006661 phosphatidylinositol biosynthetic process [PMID:12079494]
EXISTENCE:mutant phenotype:GO:0000742 karyogamy involved in conjugation with cellular fusion [PMID:12836012]
EXISTENCE:mutant phenotype:GO:0006606 protein import into nucleus [PMID:17878171]
EXISTENCE:mutant phenotype:GO:0006661 phosphatidylinositol biosynthetic process [PMID:12079494]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:27093085]
EXISTENCE:mutant phenotype:GO:0006898 receptor-mediated endocytosis [PMID:7988551|PMID:9450989]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:8310294]
EXISTENCE:mutant phenotype:GO:0007114 cell budding [PMID:8310294]
EXISTENCE:mutant phenotype:GO:0016237 lysosomal microautophagy [PMID:16055436]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:9859992]
EXISTENCE:mutant phenotype:GO:0051300 spindle pole body organization [PMID:1469052]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:10749918|PMID:8294515|PMID:9450989|PMID:9950677]
EXISTENCE:physical interaction:GO:0048306 calcium-dependent protein binding [PMID:9756868]
EXISTENCE:physical interaction:GO:0051019 mitogen-activated protein kinase binding [PMID:27421986]
Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability
chitobiosyldiphosphodolichol beta-1,4 mannosyltransferase
EXISTENCE:direct assay:GO:0019187 beta-1,4-mannosyltransferase activity [PMID:6368538]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:6369318]
EXISTENCE:mutant phenotype:GO:0006490 oligosaccharide-lipid intermediate biosynthetic process [PMID:7037780]
EXISTENCE:physical interaction:GO:0005783 endoplasmic reticulum [PMID:15044395]
Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability; human homolog ALG1 complements yeast null mutant
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19251690|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0019693 ribose phosphate metabolic process [PMID:10542272|PMID:12370179]
EXISTENCE:direct assay:GO:0047631 ADP-ribose diphosphatase activity [PMID:10542272]
Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:14718168]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:29598828]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:14718168]
EXISTENCE:direct assay:GO:0005654 nucleoplasm [PMID:14718168]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:18488019]
EXISTENCE:direct assay:GO:0070390 transcription export complex 2 [PMID:15311284]
EXISTENCE:direct assay:GO:0071819 DUBm complex [PMID:20395473]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:18003937]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:18667528]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:14718168]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:19269973]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:14718168]
EXISTENCE:mutant phenotype:GO:0071028 nuclear mRNA surveillance [PMID:18003937]
Global transcriptional regulator, component of SAGA, TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to nuclear periphery and to non-nascent mRNP; plays role in negatively regulating telomere length through modulation of H2BK123 mono-ubiquitination and its interaction with nuclear pore complex
EXISTENCE:direct assay:GO:0003713 transcription coactivator activity [PMID:9867831]
EXISTENCE:direct assay:GO:0003714 transcription corepressor activity [PMID:9741624]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:1739976]
EXISTENCE:direct assay:GO:0042826 histone deacetylase binding [PMID:14525981]
EXISTENCE:direct assay:GO:1900191 negative regulation of single-species biofilm formation [PMID:32054862]
EXISTENCE:genetic interaction:GO:0003713 transcription coactivator activity [PMID:9867831]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:18708352|PMID:9858577|PMID:9560430]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:9280286]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:26546823]
EXISTENCE:mutant phenotype:GO:2001020 regulation of response to DNA damage stimulus [PMID:9741624]
EXISTENCE:physical interaction:GO:0003713 transcription coactivator activity [PMID:9867831]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:9741624]
EXISTENCE:physical interaction:GO:0017053 transcription repressor complex [PMID:9111019]
General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+]
EXISTENCE:direct assay:GO:0000113 nucleotide-excision repair factor 4 complex [PMID:9497356]
EXISTENCE:direct assay:GO:0000715 nucleotide-excision repair, DNA damage recognition [PMID:15177043]
EXISTENCE:direct assay:GO:0003684 damaged DNA binding [PMID:9497356]
EXISTENCE:direct assay:GO:0004842 ubiquitin-protein transferase activity [PMID:16675952]
EXISTENCE:direct assay:GO:0008094 ATP-dependent activity, acting on DNA [PMID:9497356]
EXISTENCE:direct assay:GO:0008104 protein localization [PMID:15226437]
EXISTENCE:direct assay:GO:0031463 Cul3-RING ubiquitin ligase complex [PMID:16675952]
EXISTENCE:mutant phenotype:GO:0000715 nucleotide-excision repair, DNA damage recognition [PMID:15177043]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:16675952]
Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
L-aminoadipate-semialdehyde dehydrogenase
EXISTENCE:direct assay:GO:0004043 L-aminoadipate-semialdehyde dehydrogenase activity [PMID:10320345]
EXISTENCE:direct assay:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:10320345]
EXISTENCE:mutant phenotype:GO:0004043 L-aminoadipate-semialdehyde dehydrogenase activity [PMID:3023949|PMID:4401608]
EXISTENCE:mutant phenotype:GO:0019878 lysine biosynthetic process via aminoadipic acid [PMID:3023949|PMID:4401608]
Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p
EXISTENCE:mutant phenotype:GO:0006098 pentose-phosphate shunt [PMID:7916691]
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication
translation elongation factor EF-1 alpha
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:25561732]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:17925388]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:20032305]
EXISTENCE:direct assay:GO:0019003 GDP binding [PMID:17925388]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:25561732]
EXISTENCE:direct assay:GO:0051017 actin filament bundle assembly [PMID:19095653|PMID:25561732|PMID:16116436]
EXISTENCE:direct assay:GO:1904408 melatonin binding [PMID:31708896]
EXISTENCE:genetic interaction:GO:0006409 tRNA export from nucleus [PMID:10766739|PMID:17203074]
EXISTENCE:mutant phenotype:GO:0003746 translation elongation factor activity [PMID:10514524]
EXISTENCE:mutant phenotype:GO:0006409 tRNA export from nucleus [PMID:10766739|PMID:17203074]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:10514524]
EXISTENCE:mutant phenotype:GO:0051017 actin filament bundle assembly [PMID:16116436]
EXISTENCE:physical interaction:GO:0005739 mitochondrion [PMID:16962558]
Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging
EXISTENCE:direct assay:GO:0005685 U1 snRNP [PMID:9630245]
EXISTENCE:direct assay:GO:0071004 U2-type prespliceosome [PMID:16618970]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:8449403]
EXISTENCE:genetic interaction:GO:0030619 U1 snRNA binding [PMID:8449403]
EXISTENCE:physical interaction:GO:0030619 U1 snRNA binding [PMID:8849781]
U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing
EXISTENCE:direct assay:GO:0005761 mitochondrial ribosome [PMID:21670217]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:21670217]
EXISTENCE:mutant phenotype:GO:0070131 positive regulation of mitochondrial translation [PMID:21670217]
EXISTENCE:physical interaction:GO:0034551 mitochondrial respiratory chain complex III assembly [PMID:21670217]
EXISTENCE:physical interaction:GO:0043022 ribosome binding [PMID:21670217]
EXISTENCE:physical interaction:GO:0061671 Cbp3p-Cbp6 complex [PMID:21670217]
Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
EXISTENCE:direct assay:GO:0004820 glycine-tRNA ligase activity [PMID:21877692]
EXISTENCE:mutant phenotype:GO:0004820 glycine-tRNA ligase activity [PMID:10874035]
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:10874035]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:10874035]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:10224248]
EXISTENCE:mutant phenotype:GO:0006426 glycyl-tRNA aminoacylation [PMID:10874035]
EXISTENCE:mutant phenotype:GO:0070150 mitochondrial glycyl-tRNA aminoacylation [PMID:10874035]
Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant
mitochondrial 54S ribosomal protein YmL36
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:12392552]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:12392552]
EXISTENCE:mutant phenotype:GO:0032543 mitochondrial translation [PMID:19339279]
Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region
transcription factor TFIIIC subunit TFC1
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:1474578|PMID:2002052|PMID:2052571|PMID:2180956]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:3633923|PMID:8464480]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2002052]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:12533520]
Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
ARS216; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0006470 protein dephosphorylation [PMID:10580002]
Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
alpha,alpha-trehalose-phosphate synthase (UDP-forming) TPS1
EXISTENCE:direct assay:GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity [PMID:1425702]
EXISTENCE:direct assay:GO:0005946 alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [PMID:1425702]
EXISTENCE:direct assay:GO:0005992 trehalose biosynthetic process [PMID:1425702]
EXISTENCE:direct assay:GO:0044262 cellular carbohydrate metabolic process [PMID:1425702]
EXISTENCE:mutant phenotype:GO:0003825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity [PMID:9837904|PMID:1425702]
EXISTENCE:mutant phenotype:GO:0004805 trehalose-phosphatase activity [PMID:9837904]
EXISTENCE:mutant phenotype:GO:0005946 alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [PMID:1425702]
EXISTENCE:mutant phenotype:GO:0005992 trehalose biosynthetic process [PMID:1425702]
EXISTENCE:mutant phenotype:GO:0044262 cellular carbohydrate metabolic process [PMID:1425702]
EXISTENCE:mutant phenotype:GO:0071465 cellular response to desiccation [PMID:25456447]
EXISTENCE:physical interaction:GO:0005946 alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [PMID:9837904]
Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid; required for pseudohyphal growth
hypothetical protein; identified by gene-trapping, microarray analysis, and genome-wide homology searches; mRNA identified as translated by ribosome profiling data; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; partially overlaps the dubious ORF YBR126W-B
H(+)-transporting V1 sector ATPase subunit B
EXISTENCE:direct assay:GO:0000221 vacuolar proton-transporting V-type ATPase, V1 domain [PMID:2478556|PMID:2857169]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:18048916|PMID:23836928|PMID:26928762]
EXISTENCE:direct assay:GO:0045121 membrane raft [PMID:23836928]
EXISTENCE:direct assay:GO:1990816 vacuole-mitochondrion membrane contact site [PMID:25026036]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:10991947]
EXISTENCE:mutant phenotype:GO:0007035 vacuolar acidification [PMID:18048916|PMID:2141385]
EXISTENCE:mutant phenotype:GO:0046961 proton-transporting ATPase activity, rotational mechanism [PMID:2141385]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:2141385]
EXISTENCE:mutant phenotype:GO:1902906 proteasome storage granule assembly [PMID:23690178]
Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress; human homolog ATP6V1B1, implicated in autosomal-recessive distal renal tubular acidosis (RTA) with sensorineural deafness, complements yeast null mutant
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:16421251]
EXISTENCE:direct assay:GO:0000407 phagophore assembly site [PMID:16421251|PMID:23129774]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:9712845]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:9712845|PMID:11157979|PMID:32788210]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:8901576]
Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:22562153]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22562153|PMID:14562095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:22562153]
EXISTENCE:genetic interaction:GO:1902647 negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process [PMID:22562153]
EXISTENCE:mutant phenotype:GO:1902647 negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process [PMID:22562153]
hypothetical protein; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:10212145]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11032818]
EXISTENCE:direct assay:GO:1990825 sequence-specific mRNA binding [PMID:28092367]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:8625407]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:10212145|PMID:28092367]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:14691136]
Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance
EXISTENCE:direct assay:GO:0001786 phosphatidylserine binding [PMID:24413168]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:20797862|PMID:24413168]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:20797862]
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:11069774]
EXISTENCE:direct assay:GO:0006914 autophagy [PMID:12208507]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:24413168]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:24413168]
EXISTENCE:direct assay:GO:0035658 Mon1-Ccz1 complex [PMID:24413168]
EXISTENCE:direct assay:GO:0043547 positive regulation of GTPase activity [PMID:20797862|PMID:24413168]
EXISTENCE:direct assay:GO:0097352 autophagosome maturation [PMID:12208507]
EXISTENCE:genetic interaction:GO:0044395 protein targeting to vacuolar membrane [PMID:23979137]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12364329]
EXISTENCE:mutant phenotype:GO:0007034 vacuolar transport [PMID:11590240]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:19793921]
EXISTENCE:mutant phenotype:GO:0044395 protein targeting to vacuolar membrane [PMID:23979137]
EXISTENCE:mutant phenotype:GO:0048278 vesicle docking [PMID:14662743]
EXISTENCE:physical interaction:GO:0035658 Mon1-Ccz1 complex [PMID:20797862]
Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10545096]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:10545096]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10545096]
EXISTENCE:mutant phenotype:GO:1902269 positive regulation of polyamine transmembrane transport [PMID:23755272]
EXISTENCE:mutant phenotype:GO:1902274 positive regulation of (R)-carnitine transmembrane transport [PMID:23755272]
Plasma membrane regulator of polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes
EXISTENCE:direct assay:GO:0000144 cellular bud neck septin ring [PMID:10490648|PMID:27053666]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:11408575]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10805747|PMID:11408575|PMID:22842922]
EXISTENCE:direct assay:GO:0032174 cellular bud neck septin collar [PMID:23389636]
EXISTENCE:direct assay:GO:0035241 protein-arginine omega-N monomethyltransferase activity [PMID:16426232|PMID:18515076]
EXISTENCE:direct assay:GO:0035243 protein-arginine omega-N symmetric methyltransferase activity [PMID:18515076]
EXISTENCE:mutant phenotype:GO:0000086 G2/M transition of mitotic cell cycle [PMID:10490630]
EXISTENCE:mutant phenotype:GO:0000144 cellular bud neck septin ring [PMID:27053666]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:23042131]
EXISTENCE:mutant phenotype:GO:0051726 regulation of cell cycle [PMID:8647431]
Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; responsible for symmetric dimethylation of histone H4 on Arg3; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
cyclin-dependent protein kinase regulatory subunit CKS1
EXISTENCE:direct assay:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:2664468]
EXISTENCE:direct assay:GO:0030295 protein kinase activator activity [PMID:10913169]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:20855529]
EXISTENCE:direct assay:GO:0043130 ubiquitin binding [PMID:17198374]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20516216]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:15629725]
EXISTENCE:mutant phenotype:GO:0007346 regulation of mitotic cell cycle [PMID:2664468|PMID:8491379]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20516216]
Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant
EXISTENCE:direct assay:GO:0000723 telomere maintenance [PMID:15721260]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23934994]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:17202155]
EXISTENCE:genetic interaction:GO:0004672 protein kinase activity [PMID:21841787]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:10511542]
EXISTENCE:mutant phenotype:GO:0006310 DNA recombination [PMID:10511543]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:10511543]
EXISTENCE:mutant phenotype:GO:1901857 positive regulation of cellular respiration [PMID:31073026]
EXISTENCE:mutant phenotype:GO:2000105 positive regulation of DNA-templated DNA replication [PMID:23307868]
Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11266464]
EXISTENCE:direct assay:GO:0072380 TRC complex [PMID:20850366]
EXISTENCE:genetic interaction:GO:0006620 post-translational protein targeting to endoplasmic reticulum membrane [PMID:25288638]
Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene
Cytoplasmic hypothetical protein; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:14562095|PMID:26928762]
EXISTENCE:direct assay:GO:0000328 fungal-type vacuole lumen [PMID:29514932]
EXISTENCE:direct assay:GO:0017171 serine hydrolase activity [PMID:14645503]
EXISTENCE:genetic interaction:GO:0004185 serine-type carboxypeptidase activity [PMID:29514932]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:29514932]
EXISTENCE:genetic interaction:GO:0031638 zymogen activation [PMID:29514932]
EXISTENCE:genetic interaction:GO:0046938 phytochelatin biosynthetic process [PMID:17408619]
Vacuolar serine-type carboxypeptidase; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner; N-glycosylated
ARS217; Autonomously replicating sequence
EXISTENCE:mutant phenotype:GO:0005096 GTPase activator activity [PMID:2178777]
EXISTENCE:mutant phenotype:GO:0007193 adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway [PMID:2540426]
EXISTENCE:mutant phenotype:GO:0016020 membrane [PMID:1875942]
EXISTENCE:mutant phenotype:GO:0043547 positive regulation of GTPase activity [PMID:2178777]
EXISTENCE:mutant phenotype:GO:0045761 regulation of adenylate cyclase activity [PMID:1875942]
EXISTENCE:mutant phenotype:GO:0046580 negative regulation of Ras protein signal transduction [PMID:15339905]
GTPase-activating protein; negatively regulates RAS by converting it from GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis
25S rRNA (adenine2142-N1)-methyltransferase
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:23558746|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0016433 rRNA (adenine) methyltransferase activity [PMID:23558746]
Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; null mutant exhibits abnormal lipid metabolic phenotype; not an essential gene
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:13680366]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:13680366]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:13680366]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:13680366|PMID:7739558]
Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
EXISTENCE:direct assay:GO:0003747 translation release factor activity [PMID:20947765]
EXISTENCE:direct assay:GO:0006415 translational termination [PMID:20947765]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:23451152]
EXISTENCE:direct assay:GO:0018444 translation release factor complex [PMID:7556078]
EXISTENCE:genetic interaction:GO:0006415 translational termination [PMID:18724935]
EXISTENCE:mutant phenotype:GO:0006353 DNA-templated transcription termination [PMID:23109428]
EXISTENCE:mutant phenotype:GO:0006415 translational termination [PMID:9182990|PMID:18724935]
EXISTENCE:mutant phenotype:GO:0016149 translation release factor activity, codon specific [PMID:9182990]
Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
hypothetical protein; conserved across S. cerevisiae strains; YBR144C is not an essential gene
EXISTENCE:genetic interaction:GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway [PMID:12499363]
EXISTENCE:mutant phenotype:GO:0006116 NADH oxidation [PMID:15082781]
EXISTENCE:mutant phenotype:GO:0019655 glycolytic fermentation to ethanol [PMID:15082781]
Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
mitochondrial 37S ribosomal protein MRPS9
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:11278769]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:11278769]
EXISTENCE:direct assay:GO:0005763 mitochondrial small ribosomal subunit [PMID:11278769]
Mitochondrial ribosomal protein of the small subunit
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23169667]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:23169667]
EXISTENCE:mutant phenotype:GO:0034488 basic amino acid transmembrane export from vacuole [PMID:23169667]
Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:19465564]
EXISTENCE:direct assay:GO:0031105 septin complex [PMID:24390141]
EXISTENCE:mutant phenotype:GO:0032120 ascospore-type prospore membrane formation [PMID:19465564]
Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication
D-arabinose 1-dehydrogenase (NAD(P)(+)) ARA1
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9920381]
EXISTENCE:direct assay:GO:0044262 cellular carbohydrate metabolic process [PMID:9920381]
EXISTENCE:direct assay:GO:0045290 D-arabinose 1-dehydrogenase [NAD(P)+] activity [PMID:9920381]
EXISTENCE:mutant phenotype:GO:0044262 cellular carbohydrate metabolic process [PMID:9920381]
NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
hypothetical protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; associates with rDNA chromatin in vivo; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:30879301]
2Fe-2S cytosolic hypothetical protein; bishistidinyl coordinated, non-Rieske [2Fe-2S] cluster containing thiredoxin-like ferredoxin; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm; homologous to AIM32
EXISTENCE:direct assay:GO:0046540 U4/U6 x U5 tri-snRNP complex [PMID:9858581|PMID:10449419]
EXISTENCE:genetic interaction:GO:0000349 generation of catalytic spliceosome for first transesterification step [PMID:9858581]
EXISTENCE:mutant phenotype:GO:0000398 mRNA splicing, via spliceosome [PMID:9858581|PMID:10449419]
mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia; temperature-sensitive phenotype of prp38-1 mutant can be suppressed by human homolog of SPP381, MFAP1
2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase
EXISTENCE:direct assay:GO:0008703 5-amino-6-(5-phosphoribosylamino)uracil reductase activity [PMID:23620735]
EXISTENCE:mutant phenotype:GO:0008703 5-amino-6-(5-phosphoribosylamino)uracil reductase activity [PMID:4555411]
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:5366000]
Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
DNA-directed RNA polymerase core subunit RPB5
EXISTENCE:direct assay:GO:0001054 RNA polymerase I activity [PMID:9837969]
EXISTENCE:direct assay:GO:0001055 RNA polymerase II activity [PMID:8288647]
EXISTENCE:direct assay:GO:0001056 RNA polymerase III activity [PMID:3905793]
EXISTENCE:direct assay:GO:0003968 RNA-dependent RNA polymerase activity [PMID:18004386]
EXISTENCE:direct assay:GO:0005665 RNA polymerase II, core complex [PMID:1331084|PMID:2183013|PMID:2186966]
EXISTENCE:direct assay:GO:0005666 RNA polymerase III complex [PMID:10611227|PMID:3905793]
EXISTENCE:direct assay:GO:0005736 RNA polymerase I complex [PMID:11486042|PMID:9837969]
EXISTENCE:direct assay:GO:0006360 transcription by RNA polymerase I [PMID:9837969]
EXISTENCE:direct assay:GO:0042797 tRNA transcription by RNA polymerase III [PMID:3905793]
EXISTENCE:genetic interaction:GO:0006366 transcription by RNA polymerase II [PMID:9860960]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:17179178]
EXISTENCE:mutant phenotype:GO:0006366 transcription by RNA polymerase II [PMID:17179178|PMID:9860960]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:17179178]
RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
ETC8; Chromosome-organizing-clamp; tethers chromosomal regions to the nuclear periphery; binds TFIIIC transcription factor but does not recruit RNA Polymerase III; can act as a transcription-blocking insulator or as a heterochromatin barrier element; located between divergently transcribed genes RPB5 and CNS1
EXISTENCE:direct assay:GO:0030544 Hsp70 protein binding [PMID:15044454]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:25380751]
EXISTENCE:direct assay:GO:0051879 Hsp90 protein binding [PMID:12788914]
EXISTENCE:genetic interaction:GO:0042026 protein refolding [PMID:9819421]
EXISTENCE:physical interaction:GO:0005737 cytoplasm [PMID:15044454]
EXISTENCE:physical interaction:GO:0006457 protein folding [PMID:15044454]
TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant
EXISTENCE:direct assay:GO:0005828 kinetochore microtubule [PMID:11724818]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:11724818]
EXISTENCE:direct assay:GO:0032133 chromosome passenger complex [PMID:19158380]
EXISTENCE:direct assay:GO:0051233 spindle midzone [PMID:12566427]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:10385519]
EXISTENCE:mutant phenotype:GO:0030295 protein kinase activator activity [PMID:10385519]
EXISTENCE:mutant phenotype:GO:0032185 septin cytoskeleton organization [PMID:17652458]
EXISTENCE:mutant phenotype:GO:0032465 regulation of cytokinesis [PMID:16615892]
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
hypothetical protein; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12628189]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12628189]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12628189]
EXISTENCE:genetic interaction:GO:0001100 negative regulation of exit from mitosis [PMID:12628189]
EXISTENCE:genetic interaction:GO:2001042 negative regulation of septum digestion after cytokinesis [PMID:30273335]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:12628189]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:12628189]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:30273335]
EXISTENCE:mutant phenotype:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:12628189]
EXISTENCE:mutant phenotype:GO:0032984 protein-containing complex disassembly [PMID:12628189]
EXISTENCE:mutant phenotype:GO:2001042 negative regulation of septum digestion after cytokinesis [PMID:30273335]
EXISTENCE:physical interaction:GO:0031267 small GTPase binding [PMID:12628189]
Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12087109]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:23263984]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:23263984]
EXISTENCE:mutant phenotype:GO:0030497 fatty acid elongation [PMID:12087109]
EXISTENCE:mutant phenotype:GO:0042761 very long-chain fatty acid biosynthetic process [PMID:23263984]
EXISTENCE:mutant phenotype:GO:0045703 ketoreductase activity [PMID:12087109]
Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
cyclin-dependent serine/threonine-protein kinase CDC28
EXISTENCE:direct assay:GO:0000307 cyclin-dependent protein kinase holoenzyme complex [PMID:1849458|PMID:2142620]
EXISTENCE:direct assay:GO:0000723 telomere maintenance [PMID:24164896]
EXISTENCE:direct assay:GO:0000993 RNA polymerase II complex binding [PMID:22689984]
EXISTENCE:direct assay:GO:0004693 cyclin-dependent protein serine/threonine kinase activity [PMID:2142620|PMID:27068241]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14685274]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:3312233]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:17560371]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12554645]
EXISTENCE:direct assay:GO:0010571 positive regulation of nuclear cell cycle DNA replication [PMID:14747467]
EXISTENCE:direct assay:GO:0045892 negative regulation of DNA-templated transcription [PMID:14993267]
EXISTENCE:direct assay:GO:0045893 positive regulation of DNA-templated transcription [PMID:1652372]
EXISTENCE:direct assay:GO:0045930 negative regulation of mitotic cell cycle [PMID:10074450]
EXISTENCE:direct assay:GO:0051446 positive regulation of meiotic cell cycle [PMID:16814718]
EXISTENCE:direct assay:GO:1905634 regulation of protein localization to chromatin [PMID:25602519]
EXISTENCE:genetic interaction:GO:0000706 meiotic DNA double-strand break processing [PMID:20150422]
EXISTENCE:genetic interaction:GO:0010568 regulation of budding cell apical bud growth [PMID:17417630]
EXISTENCE:genetic interaction:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:16688214]
EXISTENCE:genetic interaction:GO:0010898 positive regulation of triglyceride catabolic process [PMID:19150427]
EXISTENCE:genetic interaction:GO:0030163 protein catabolic process [PMID:21993622]
EXISTENCE:genetic interaction:GO:0032210 regulation of telomere maintenance via telomerase [PMID:19135888]
EXISTENCE:genetic interaction:GO:0045893 positive regulation of DNA-templated transcription [PMID:1652372]
EXISTENCE:genetic interaction:GO:0090307 mitotic spindle assembly [PMID:21558801]
EXISTENCE:genetic interaction:GO:1901319 positive regulation of trehalose catabolic process [PMID:27203179]
EXISTENCE:genetic interaction:GO:1902596 negative regulation of DNA replication origin binding [PMID:29388912]
EXISTENCE:genetic interaction:GO:1990758 mitotic sister chromatid biorientation [PMID:22056777]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:22056777]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:21841787]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:22521784|PMID:21841787|PMID:8930895]
EXISTENCE:mutant phenotype:GO:0006303 double-strand break repair via nonhomologous end joining [PMID:22563681]
EXISTENCE:mutant phenotype:GO:0006370 7-methylguanosine mRNA capping [PMID:22689984]
EXISTENCE:mutant phenotype:GO:0006892 post-Golgi vesicle-mediated transport [PMID:22767578]
EXISTENCE:mutant phenotype:GO:0007130 synaptonemal complex assembly [PMID:20825495]
EXISTENCE:mutant phenotype:GO:0010568 regulation of budding cell apical bud growth [PMID:17417630]
EXISTENCE:mutant phenotype:GO:0010570 regulation of filamentous growth [PMID:9891070]
EXISTENCE:mutant phenotype:GO:0010571 positive regulation of nuclear cell cycle DNA replication [PMID:12783856|PMID:14747467|PMID:4580573]
EXISTENCE:mutant phenotype:GO:0010696 positive regulation of mitotic spindle pole body separation [PMID:16688214|PMID:8887667]
EXISTENCE:mutant phenotype:GO:0010898 positive regulation of triglyceride catabolic process [PMID:19150427]
EXISTENCE:mutant phenotype:GO:0030163 protein catabolic process [PMID:21098119]
EXISTENCE:mutant phenotype:GO:0032880 regulation of protein localization [PMID:29263158]
EXISTENCE:mutant phenotype:GO:0034504 protein localization to nucleus [PMID:19188495]
EXISTENCE:mutant phenotype:GO:0045819 positive regulation of glycogen catabolic process [PMID:27203179]
EXISTENCE:mutant phenotype:GO:0045875 negative regulation of sister chromatid cohesion [PMID:21549314]
EXISTENCE:mutant phenotype:GO:0045892 negative regulation of DNA-templated transcription [PMID:12081645|PMID:14993267]
EXISTENCE:mutant phenotype:GO:0045931 positive regulation of mitotic cell cycle [PMID:2165600|PMID:7002718]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:22689984]
EXISTENCE:mutant phenotype:GO:0051446 positive regulation of meiotic cell cycle [PMID:16814718|PMID:2680756]
EXISTENCE:mutant phenotype:GO:0051447 negative regulation of meiotic cell cycle [PMID:12081645]
EXISTENCE:mutant phenotype:GO:0090169 regulation of spindle assembly [PMID:21558801]
EXISTENCE:mutant phenotype:GO:1901319 positive regulation of trehalose catabolic process [PMID:27203179]
EXISTENCE:mutant phenotype:GO:1902889 protein localization to spindle microtubule [PMID:22521784]
EXISTENCE:mutant phenotype:GO:1903500 negative regulation of mitotic actomyosin contractile ring assembly [PMID:24413167]
EXISTENCE:mutant phenotype:GO:1904291 positive regulation of mitotic DNA damage checkpoint [PMID:15496928]
EXISTENCE:mutant phenotype:GO:1905168 positive regulation of double-strand break repair via homologous recombination [PMID:15496928]
EXISTENCE:mutant phenotype:GO:1990139 protein localization to nuclear periphery [PMID:20702586]
EXISTENCE:mutant phenotype:GO:2001033 negative regulation of double-strand break repair via nonhomologous end joining [PMID:19699692]
Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant
mannosylinositol phosphorylceramide synthase catalytic subunit CSH1
EXISTENCE:mutant phenotype:GO:0006688 glycosphingolipid biosynthetic process [PMID:12954640]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:12954640]
EXISTENCE:mutant phenotype:GO:0103064 inositol phosphorylceramide mannosyltransferase activity [PMID:12954640]
Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:11935221|PMID:15781460]
Covalently-bound cell wall hypothetical protein; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762]
EXISTENCE:genetic interaction:GO:0009306 protein secretion [PMID:10469658]
hypothetical protein; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
EXISTENCE:direct assay:GO:0045145 single-stranded DNA 5'-3' exodeoxyribonuclease activity [PMID:20086101]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:20086101]
Mitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:9388248]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:15077113|PMID:15564045]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:15975906|PMID:23345439]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:15975906|PMID:26928762]
EXISTENCE:genetic interaction:GO:0016236 macroautophagy [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:12052896|PMID:12210899]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:12052896]
EXISTENCE:mutant phenotype:GO:0016192 vesicle-mediated transport [PMID:11840166]
EXISTENCE:mutant phenotype:GO:0016236 macroautophagy [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0031503 protein-containing complex localization [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0034067 protein localization to Golgi apparatus [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0034497 protein localization to phagophore assembly site [PMID:27462928]
EXISTENCE:mutant phenotype:GO:0034976 response to endoplasmic reticulum stress [PMID:26966233]
EXISTENCE:mutant phenotype:GO:0043001 Golgi to plasma membrane protein transport [PMID:16926193]
Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10991951]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:10991951]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:10991951]
Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
EXISTENCE:direct assay:GO:0006571 tyrosine biosynthetic process [PMID:30771296]
EXISTENCE:direct assay:GO:0008977 prephenate dehydrogenase (NAD+) activity [PMID:30771296]
EXISTENCE:mutant phenotype:GO:0006570 tyrosine metabolic process [PMID:2697638]
EXISTENCE:mutant phenotype:GO:0008977 prephenate dehydrogenase (NAD+) activity [PMID:2697638]
Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:26135751]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9618478|PMID:9620854]
EXISTENCE:direct assay:GO:0005697 telomerase holoenzyme complex [PMID:27156450]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0000172 ribonuclease MRP complex [PMID:9618478]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9618478|PMID:9620854]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9618478|PMID:9620854]
Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:14617799]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:14617799]
Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
EXISTENCE:direct assay:GO:0000774 adenyl-nucleotide exchange factor activity [PMID:16688212]
EXISTENCE:genetic interaction:GO:0010499 proteasomal ubiquitin-independent protein catabolic process [PMID:29507114]
EXISTENCE:genetic interaction:GO:0042026 protein refolding [PMID:16688211]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:29507114]
Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000837 Doa10p ubiquitin ligase complex [PMID:16873066]
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873065|PMID:16873066]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922|PMID:26928762]
EXISTENCE:direct assay:GO:0006274 DNA replication termination [PMID:31509741]
EXISTENCE:direct assay:GO:0030894 replisome [PMID:31509741]
EXISTENCE:direct assay:GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex [PMID:11598205|PMID:11733065]
EXISTENCE:direct assay:GO:0036266 Cdc48p-Npl4p-Vms1p AAA ATPase complex [PMID:21070972]
EXISTENCE:direct assay:GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network [PMID:11598205]
EXISTENCE:direct assay:GO:1990112 RQC complex [PMID:23178123]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:11739805]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:11740563]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:20206597]
EXISTENCE:mutant phenotype:GO:0051228 mitotic spindle disassembly [PMID:14636562]
EXISTENCE:mutant phenotype:GO:0051974 negative regulation of telomerase activity [PMID:26365526]
EXISTENCE:mutant phenotype:GO:0070651 nonfunctional rRNA decay [PMID:22505030]
EXISTENCE:mutant phenotype:GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system [PMID:18812321]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:11740563|PMID:11813000]
EXISTENCE:mutant phenotype:GO:0072665 protein localization to vacuole [PMID:29444958]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579|PMID:21070972]
EXISTENCE:mutant phenotype:GO:0099638 endosome to plasma membrane protein transport [PMID:36125415]
EXISTENCE:mutant phenotype:GO:1900182 positive regulation of protein localization to nucleus [PMID:11598205|PMID:11733065]
EXISTENCE:mutant phenotype:GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process [PMID:23358411]
Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; K48-specific Ub chain binding receptor that assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates destined for proteasomal degradation; role in regulated destruction of ER membrane proteins, like HMG-CoA reductase (Hmg1/2p), cytosolic proteins (Fbp1p) and mislocalized proteins (Cse4p); mobilizes membrane-bound transcription factors by regulated Ub/proteasome-dependent processing; contains a UBX domain
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:26928762|PMID:27831485]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:9275186]
EXISTENCE:direct assay:GO:0008320 protein transmembrane transporter activity [PMID:9252322]
EXISTENCE:direct assay:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:7758110]
EXISTENCE:genetic interaction:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:32513868]
EXISTENCE:mutant phenotype:GO:0030447 filamentous growth [PMID:15645503]
EXISTENCE:mutant phenotype:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:8257795|PMID:32513868]
EXISTENCE:physical interaction:GO:0031207 Sec62/Sec63 complex [PMID:8257795]
Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and translocation into the ER; other members are Sec63p, Sec62p, and Sec72p
EXISTENCE:direct assay:GO:0005515 protein binding [PMID:36288698]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:36288698]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:17973654]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17973654]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:36288698]
EXISTENCE:mutant phenotype:GO:0032984 protein-containing complex disassembly [PMID:36288698]
EXISTENCE:mutant phenotype:GO:0071630 nuclear protein quality control by the ubiquitin-proteasome system [PMID:36288698]
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17973654]
GYF domain protein; involved in COPII vesicle formation; regulates Cdc48p function in transcription stress response; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; homologous to human GIGYF1 and GIGYF2; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11922673]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11922673]
EXISTENCE:direct assay:GO:0006974 cellular response to DNA damage stimulus [PMID:10975253]
EXISTENCE:genetic interaction:GO:0043248 proteasome assembly [PMID:17431397]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:9491890]
EXISTENCE:mutant phenotype:GO:0043248 proteasome assembly [PMID:9491890]
Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:11742990]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11687631|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:1903341 regulation of meiotic DNA double-strand break formation [PMID:23246437]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11752412|PMID:11742990]
Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
3-methyl-2-oxobutanoate hydroxymethyltransferase
EXISTENCE:mutant phenotype:GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity [PMID:11154694]
EXISTENCE:mutant phenotype:GO:0015940 pantothenate biosynthetic process [PMID:11154694]
Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
medium-chain fatty acid ethyl ester synthase/esterase
EXISTENCE:direct assay:GO:0034319 alcohol O-butanoyltransferase activity [PMID:16361250]
EXISTENCE:direct assay:GO:0034338 short-chain carboxylesterase activity [PMID:16361250]
EXISTENCE:direct assay:GO:0051792 medium-chain fatty acid biosynthetic process [PMID:16361250]
EXISTENCE:mutant phenotype:GO:0044255 cellular lipid metabolic process [PMID:10515935]
EXISTENCE:mutant phenotype:GO:0051792 medium-chain fatty acid biosynthetic process [PMID:16361250]
EXISTENCE:mutant phenotype:GO:0051793 medium-chain fatty acid catabolic process [PMID:16361250]
Octanoyl-CoA:ethanol acyltransferase; also functions as thioesterase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:19812251]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:11266460|PMID:9685359|PMID:9786948|PMID:16407407]
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11266460]
EXISTENCE:genetic interaction:GO:0048312 intracellular distribution of mitochondria [PMID:23341591]
EXISTENCE:mutant phenotype:GO:0008053 mitochondrial fusion [PMID:9786948|PMID:12808034]
EXISTENCE:physical interaction:GO:0008053 mitochondrial fusion [PMID:12808034]
Mitofusin, protein involved in mitochondrial outer membrane fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system; activity regulated by ubiquitylation at conserved lysine residues and by deubiquitylases Ubp2p and Ubp12p
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:12455697|PMID:15755916]
EXISTENCE:mutant phenotype:GO:0005275 amine transmembrane transporter activity [PMID:12455697]
EXISTENCE:mutant phenotype:GO:0015837 amine transport [PMID:12455697]
EXISTENCE:mutant phenotype:GO:0030476 ascospore wall assembly [PMID:12455697]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:12455697]
Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0003700 DNA-binding transcription factor activity []
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12482976]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12482976]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:10064699]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:9121433|PMID:19158363]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12482976]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:12482976]
MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:10900202|PMID:26928762]
EXISTENCE:direct assay:GO:0032541 cortical endoplasmic reticulum [PMID:24866405]
EXISTENCE:genetic interaction:GO:0046513 ceramide biosynthetic process [PMID:11694577]
EXISTENCE:genetic interaction:GO:0050291 sphingosine N-acyltransferase activity [PMID:11694577]
EXISTENCE:mutant phenotype:GO:0046514 ceramide catabolic process [PMID:10702247]
EXISTENCE:mutant phenotype:GO:0050291 sphingosine N-acyltransferase activity [PMID:10702247]
EXISTENCE:mutant phenotype:GO:0070774 phytoceramidase activity [PMID:10702247|PMID:10900202]
Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication
hypothetical protein; YBR184W is not an essential gene
EXISTENCE:direct assay:GO:0005743 mitochondrial inner membrane [PMID:11381092]
EXISTENCE:direct assay:GO:0007007 inner mitochondrial membrane organization [PMID:11381092]
EXISTENCE:direct assay:GO:0097177 mitochondrial ribosome binding [PMID:16601683]
EXISTENCE:genetic interaction:GO:0070131 positive regulation of mitochondrial translation [PMID:20427570]
EXISTENCE:mutant phenotype:GO:0032979 protein insertion into mitochondrial inner membrane from matrix [PMID:16601683]
Membrane-associated mitoribosome receptor; involved along with Dpi29p in the cotranslational insertion of nascent polypeptide chains into the inner mitochondrial membrane; associates with nascent polypeptide chains; interacts with Mdm38p and may recruit mRNA-specific translational activators to the mitoribosome; null mutant displays decreased tolerance to mistranslation-induced proteotoxic stress; localizes to the inner mitochondrial membrane
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11029058]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:24367111]
EXISTENCE:genetic interaction:GO:0042138 meiotic DNA double-strand break formation [PMID:22745819]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:18305165]
EXISTENCE:mutant phenotype:GO:0016887 ATP hydrolysis activity [PMID:24367111]
EXISTENCE:mutant phenotype:GO:0042138 meiotic DNA double-strand break formation [PMID:22745819]
EXISTENCE:mutant phenotype:GO:0051598 meiotic recombination checkpoint signaling [PMID:22072981]
Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation
putative ribosome biosynthesis protein GDT1
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:14562095]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:22842922]
EXISTENCE:direct assay:GO:0005384 manganese ion transmembrane transporter activity [PMID:29632074]
EXISTENCE:direct assay:GO:0005797 Golgi medial cisterna [PMID:23569283]
EXISTENCE:direct assay:GO:0005801 cis-Golgi network [PMID:23569283]
EXISTENCE:direct assay:GO:0015085 calcium ion transmembrane transporter activity [PMID:27075443]
EXISTENCE:direct assay:GO:0070588 calcium ion transmembrane transport [PMID:27075443]
EXISTENCE:direct assay:GO:0071421 manganese ion transmembrane transport [PMID:29632074]
EXISTENCE:genetic interaction:GO:0006874 cellular calcium ion homeostasis [PMID:23569283]
EXISTENCE:genetic interaction:GO:0030026 cellular manganese ion homeostasis [PMID:29632074]
EXISTENCE:mutant phenotype:GO:0006874 cellular calcium ion homeostasis [PMID:23569283]
EXISTENCE:mutant phenotype:GO:0030026 cellular manganese ion homeostasis [PMID:29632074]
EXISTENCE:mutant phenotype:GO:0032468 Golgi calcium ion homeostasis [PMID:27075443]
Calcium and manganese transporter with higher affinity for Ca2+; involved in Ca2+ and Mn2+ homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion localizes to the vacuole; required for the Mn2+-dependent function of glycosylation enzymes; TMEM165, a human ortholog linked to Congenital Disorders of Glycosylation, functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression regulated by Gcr1p
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:11018040]
EXISTENCE:direct assay:GO:0000974 Prp19 complex [PMID:11018040]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:11018040]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:11018040]
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
EXISTENCE:mutant phenotype:GO:0045903 positive regulation of translational fidelity [PMID:3522920|PMID:6273576|PMID:7031258|PMID:7031259]
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:7891656|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0006864 pyrimidine nucleotide transport [PMID:16194150]
EXISTENCE:direct assay:GO:0015218 pyrimidine nucleotide transmembrane transporter activity [PMID:16194150]
EXISTENCE:direct assay:GO:0030001 metal ion transport [PMID:23800229]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:16194150]
EXISTENCE:mutant phenotype:GO:0000002 mitochondrial genome maintenance [PMID:7891656]
Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
EXISTENCE:direct assay:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15705057]
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:12054864]
EXISTENCE:direct assay:GO:0017025 TBP-class protein binding [PMID:16964259]
EXISTENCE:mutant phenotype:GO:0030674 protein-macromolecule adaptor activity [PMID:18381891]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:18381891]
EXISTENCE:physical interaction:GO:0003714 transcription corepressor activity [PMID:16528100]
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11238915]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11238915]
EXISTENCE:direct assay:GO:0006335 DNA replication-dependent chromatin assembly [PMID:9030687]
EXISTENCE:direct assay:GO:0033186 CAF-1 complex [PMID:9030687]
EXISTENCE:direct assay:GO:0042393 histone binding [PMID:16503640]
Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16962558|PMID:24769239]
EXISTENCE:mutant phenotype:GO:0004347 glucose-6-phosphate isomerase activity [PMID:3007940]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:3020369]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:3007940]
Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:32034123]
hypothetical protein; identified by fungal homology and RT-PCR; ORF emerged de novo in thymine-rich ancestral non-genic region, accumulated substantial changes under positive selection, and progressively increased its transmembrane propensity to give rise to a protein that integrates into the endoplasmic reticulum (ER) membrane while retaining potential for adaptive change
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000124 SAGA complex [PMID:9674426]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:12600943]
EXISTENCE:direct assay:GO:0005669 transcription factor TFIID complex [PMID:10788514|PMID:15448131]
EXISTENCE:direct assay:GO:0006366 transcription by RNA polymerase II [PMID:12138208|PMID:15448131]
EXISTENCE:mutant phenotype:GO:0060090 molecular adaptor activity [PMID:11585915]
Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
EXISTENCE:direct assay:GO:0000030 mannosyltransferase activity [PMID:26296208]
Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain
phosphatidylinositol-3-phosphate-binding protein BEM1
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:9128251|PMID:11927541]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11927541]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:24390141]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11927541]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11927541]
EXISTENCE:direct assay:GO:0005938 cell cortex [PMID:11927541]
EXISTENCE:direct assay:GO:0030427 site of polarized growth [PMID:23613772]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:11557775]
EXISTENCE:mutant phenotype:GO:0000753 cell morphogenesis involved in conjugation with cellular fusion [PMID:10087263]
EXISTENCE:mutant phenotype:GO:0045185 maintenance of protein location [PMID:11927541]
EXISTENCE:mutant phenotype:GO:0061191 positive regulation of vacuole fusion, non-autophagic [PMID:16854988]
EXISTENCE:physical interaction:GO:0060090 molecular adaptor activity [PMID:11113154]
Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
ARS220; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex [PMID:16873066]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:8631297]
EXISTENCE:direct assay:GO:0051787 misfolded protein binding [PMID:24292014]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:23363603|PMID:8631297]
EXISTENCE:mutant phenotype:GO:0030970 retrograde protein transport, ER to cytosol [PMID:24292014]
EXISTENCE:mutant phenotype:GO:0070843 misfolded protein transport [PMID:24292014]
EXISTENCE:physical interaction:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:16619026]
ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into ER membrane and routing them to Hrd1p for ubiquitination; function normally requires N-terminal acetylation by NatB; N- and C- termini protrude into cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1
mini-chromosome maintenance complex protein 7
EXISTENCE:direct assay:GO:0003678 DNA helicase activity [PMID:15723534]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:11756674|PMID:16824194|PMID:9288745]
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:12480933]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10704410|PMID:7708676]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10704410|PMID:7708676]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:15723534|PMID:13679365]
EXISTENCE:direct assay:GO:0009378 four-way junction helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0017116 single-stranded DNA helicase activity [PMID:15723534|PMID:18657510]
EXISTENCE:direct assay:GO:0043138 3'-5' DNA helicase activity [PMID:13679365]
EXISTENCE:direct assay:GO:0097373 MCM core complex [PMID:13679365]
EXISTENCE:direct assay:GO:1904931 MCM complex binding [PMID:26912723]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:10783164|PMID:2044962]
EXISTENCE:genetic interaction:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10783164]
EXISTENCE:mutant phenotype:GO:0006270 DNA replication initiation [PMID:10783164|PMID:2044962]
EXISTENCE:mutant phenotype:GO:0006271 DNA strand elongation involved in DNA replication [PMID:10783164|PMID:10834843]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:19064704]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:19064704]
EXISTENCE:mutant phenotype:GO:0033260 nuclear DNA replication [PMID:2044962]
Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
EXISTENCE:mutant phenotype:GO:0007165 signal transduction [PMID:12661761]
Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
ARS221; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004806 triglyceride lipase activity [PMID:21478434]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:21478430|PMID:24868093]
EXISTENCE:direct assay:GO:0016788 hydrolase activity, acting on ester bonds [PMID:21478434]
EXISTENCE:mutant phenotype:GO:0055088 lipid homeostasis [PMID:21478434]
Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
EXISTENCE:genetic interaction:GO:0006491 N-glycan processing [PMID:9182588]
EXISTENCE:genetic interaction:GO:0006493 protein O-linked glycosylation [PMID:9182588]
EXISTENCE:mutant phenotype:GO:0000030 mannosyltransferase activity [PMID:9182588]
Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10608875|PMID:23836928|PMID:14562095]
EXISTENCE:genetic interaction:GO:0034755 iron ion transmembrane transport [PMID:10608875]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11378903]
Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
bifunctional urea carboxylase/allophanate hydrolase
EXISTENCE:mutant phenotype:GO:0004039 allophanate hydrolase activity [PMID:6105114]
EXISTENCE:mutant phenotype:GO:0004847 urea carboxylase activity [PMID:6105114]
EXISTENCE:mutant phenotype:GO:0043419 urea catabolic process [PMID:6105114]
Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress
hypothetical protein; conserved across S. cerevisiae strains; YBR209W is not an essential gene
Cysteine tRNA (tRNA-Cys), predicted by tRNAscan-SE analysis
Glutamate tRNA (tRNA-Glu), predicted by tRNAscan-SE analysis; thiolation of uridine at wobble position (34) requires Ncs6p
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:17298976]
EXISTENCE:genetic interaction:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:17298976]
Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000776 kinetochore [PMID:14633972]
EXISTENCE:direct assay:GO:0000817 COMA complex [PMID:14633972]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:22842922]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:14633972]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19597337]
EXISTENCE:physical interaction:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:14633972]
Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0000932 P-body [PMID:16885161]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:14570877|PMID:23222640]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:18981231|PMID:19470581|PMID:26777405]
EXISTENCE:direct assay:GO:0048471 perinuclear region of cytoplasm [PMID:14570877]
EXISTENCE:direct assay:GO:0061158 3'-UTR-mediated mRNA destabilization [PMID:14570877]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:14570877]
EXISTENCE:mutant phenotype:GO:0061158 3'-UTR-mediated mRNA destabilization [PMID:14570877]
RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase MET8
EXISTENCE:direct assay:GO:0004325 ferrochelatase activity [PMID:11980703]
EXISTENCE:direct assay:GO:0043115 precorrin-2 dehydrogenase activity [PMID:10051442|PMID:11980703]
EXISTENCE:mutant phenotype:GO:0000103 sulfate assimilation [PMID:10051442]
EXISTENCE:mutant phenotype:GO:0019354 siroheme biosynthetic process [PMID:9003798]
Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:11914276]
EXISTENCE:genetic interaction:GO:0000920 septum digestion after cytokinesis [PMID:16041152]
Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005634 nucleus [PMID:16449659]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:1406694]
Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
EXISTENCE:direct assay:GO:0015035 protein-disulfide reductase activity [PMID:28628095]
EXISTENCE:direct assay:GO:0065003 protein-containing complex assembly [PMID:28628095]
EXISTENCE:direct assay:GO:0140297 DNA-binding transcription factor binding [PMID:28628095]
EXISTENCE:mutant phenotype:GO:0018158 protein oxidation [PMID:28628095]
Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0006501 C-terminal protein lipidation [PMID:17986448]
EXISTENCE:direct assay:GO:0008047 enzyme activator activity [PMID:23503366]
EXISTENCE:direct assay:GO:0019776 Atg8 ligase activity [PMID:17986448]
EXISTENCE:direct assay:GO:0034274 Atg12-Atg5-Atg16 complex [PMID:11897782]
EXISTENCE:mutant phenotype:GO:0000045 autophagosome assembly [PMID:16874032]
EXISTENCE:mutant phenotype:GO:0032258 cytoplasm to vacuole transport by the Cvt pathway [PMID:9759731]
EXISTENCE:mutant phenotype:GO:0061739 protein lipidation involved in autophagosome assembly [PMID:24485455]
EXISTENCE:physical interaction:GO:0031386 protein tag [PMID:10233150|PMID:10508157|PMID:9759731]
Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; homolog of mammalian ULK1
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:196563|PMID:2665820]
EXISTENCE:mutant phenotype:GO:0004736 pyruvate carboxylase activity [PMID:2039506]
EXISTENCE:mutant phenotype:GO:0006094 gluconeogenesis [PMID:8185321]
Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
hypothetical protein; YBR219C is not an essential gene
hypothetical protein; YBR220C is not an essential gene
pyruvate dehydrogenase (acetyl-transferring) subunit E1 beta
EXISTENCE:direct assay:GO:0005967 mitochondrial pyruvate dehydrogenase complex [PMID:2007123]
EXISTENCE:direct assay:GO:0006086 acetyl-CoA biosynthetic process from pyruvate [PMID:7947791]
EXISTENCE:direct assay:GO:0042645 mitochondrial nucleoid [PMID:15692048]
EXISTENCE:mutant phenotype:GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity [PMID:8433986]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:8433986]
E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria
EXISTENCE:direct assay:GO:0005778 peroxisomal membrane [PMID:8841414]
EXISTENCE:direct assay:GO:0005782 peroxisomal matrix [PMID:8841414]
EXISTENCE:direct assay:GO:0033611 oxalate catabolic process [PMID:24291261]
EXISTENCE:direct assay:GO:0050203 oxalate-CoA ligase activity [PMID:24291261]
EXISTENCE:mutant phenotype:GO:0033611 oxalate catabolic process [PMID:24291261]
EXISTENCE:mutant phenotype:GO:0050203 oxalate-CoA ligase activity [PMID:24291261]
Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12206772]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:29523818]
EXISTENCE:direct assay:GO:0017005 3'-tyrosyl-DNA phosphodiesterase activity [PMID:10521354]
EXISTENCE:direct assay:GO:0070260 5'-tyrosyl-DNA phosphodiesterase activity [PMID:16751265]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:15135727|PMID:10521354|PMID:16751265]
Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topo I and topo II; mutations in the human homolog, TDP1, result in the a neurodegenerative disease, spinocerebellar ataxia with axonal neuropathy (SCAN1); yeast cells and human rhabdomyosarcoma lines that overexpress TDP1 both exhibit elevated dosage chromosomal instability (dCIN)
hypothetical protein; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
EXISTENCE:direct assay:GO:0005524 ATP binding [PMID:25957689]
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:9827555]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:25957689]
EXISTENCE:direct assay:GO:1904287 positive regulation of protein-pyridoxal-5-phosphate linkage [PMID:25957689]
EXISTENCE:mutant phenotype:GO:0030150 protein import into mitochondrial matrix [PMID:27398316]
EXISTENCE:mutant phenotype:GO:0042026 protein refolding [PMID:16460754]
Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis
EXISTENCE:direct assay:GO:0017108 5'-flap endonuclease activity [PMID:12832395]
EXISTENCE:genetic interaction:GO:0006261 DNA-templated DNA replication [PMID:12832395]
EXISTENCE:physical interaction:GO:0033557 Slx1-Slx4 complex [PMID:12832395]
Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0005788 endoplasmic reticulum lumen [PMID:16373354]
EXISTENCE:genetic interaction:GO:0070880 fungal-type cell wall beta-glucan biosynthetic process [PMID:9430631]
EXISTENCE:mutant phenotype:GO:0006491 N-glycan processing [PMID:16373354]
EXISTENCE:mutant phenotype:GO:0033919 glucan 1,3-alpha-glucosidase activity [PMID:16373354]
EXISTENCE:physical interaction:GO:0017177 glucosidase II complex [PMID:16373354]
Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16689936]
EXISTENCE:direct assay:GO:1990593 nascent polypeptide-associated complex binding [PMID:25487825]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:25487825]
EXISTENCE:mutant phenotype:GO:0033750 ribosome localization [PMID:25487825]
Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources
Mitochondrial hypothetical protein; YBR230W-A has a paralog, COQ8, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000395 mRNA 5'-splice site recognition [PMID:18084028]
EXISTENCE:direct assay:GO:0030627 pre-mRNA 5'-splice site binding [PMID:18084028]
EXISTENCE:genetic interaction:GO:0000398 mRNA splicing, via spliceosome [PMID:11158289]
U2 spliceosomal RNA (U2 snRNA), component of the spliceosome; pairs with the branchpoint sequence; functionally equivalent to mammalian U2 snRNA; stress-induced pseudouridylations at positions 56 and 93 contributes to regulation of splicing
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:15351640|PMID:23222640]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:23222640|PMID:22842922]
RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:direct assay:GO:0008017 microtubule binding [PMID:15664196|PMID:16777964]
EXISTENCE:direct assay:GO:0008608 attachment of spindle microtubules to kinetochore [PMID:17620411]
EXISTENCE:direct assay:GO:0031116 positive regulation of microtubule polymerization [PMID:15664196]
EXISTENCE:direct assay:GO:0042729 DASH complex [PMID:15640796|PMID:15664196]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:17620411|PMID:20479465]
EXISTENCE:direct assay:GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore [PMID:20479465|PMID:20479468]
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
EXISTENCE:direct assay:GO:0005885 Arp2/3 protein complex [PMID:10377407|PMID:15485833]
EXISTENCE:mutant phenotype:GO:0044396 actin cortical patch organization [PMID:15485833]
EXISTENCE:mutant phenotype:GO:2000601 positive regulation of Arp2/3 complex-mediated actin nucleation [PMID:15485833]
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:23022132]
EXISTENCE:genetic interaction:GO:0055075 potassium ion homeostasis [PMID:23022132]
EXISTENCE:mutant phenotype:GO:0015379 potassium:chloride symporter activity [PMID:23022132]
EXISTENCE:mutant phenotype:GO:0034486 vacuolar transmembrane transport [PMID:23022132]
Vacuolar membrane cation-chloride cotransporter (CCC); involved in potassium homeostasis and salt tolerance; localizes to sites of contact between vacuole and mitochondria (vCLAMPs); targeted to vacuole via AP-3 pathway; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
EXISTENCE:direct assay:GO:0004482 mRNA (guanine-N7-)-methyltransferase activity [PMID:7623811]
EXISTENCE:direct assay:GO:0006370 7-methylguanosine mRNA capping [PMID:7623811]
Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia
EXISTENCE:direct assay:GO:0008186 ATP-dependent activity, acting on RNA [PMID:8969184]
EXISTENCE:mutant phenotype:GO:0000348 mRNA branch site recognition [PMID:8405998]
RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
EXISTENCE:direct assay:GO:0000932 P-body [PMID:23222640]
EXISTENCE:direct assay:GO:0031966 mitochondrial membrane [PMID:17194787]
EXISTENCE:genetic interaction:GO:0009060 aerobic respiration [PMID:17194787]
Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:25123508|PMID:19111667|PMID:19158363]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:25123508]
EXISTENCE:genetic interaction:GO:0061414 positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:25123508]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:25123508]
EXISTENCE:mutant phenotype:GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific [PMID:25123508]
EXISTENCE:mutant phenotype:GO:0061415 negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source [PMID:25123508]
Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:22404710]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:16194233]
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:19013460]
EXISTENCE:mutant phenotype:GO:0090180 positive regulation of thiamine biosynthetic process [PMID:1551422]
Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:23708375|PMID:14562095|PMID:26928762]
Putative transporter, member of the sugar porter family; localizes to the vacuolar membrane; non-essential gene; upregulated by and required for tolerance to 4-Methylcyclohexane methanol; YBR241C has a paralog, VPS73, that arose from the whole genome duplication
ARS222; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0002953 5'-deoxynucleotidase activity [PMID:29752939]
EXISTENCE:direct assay:GO:0009159 deoxyribonucleoside monophosphate catabolic process [PMID:29752939]
EXISTENCE:direct assay:GO:0050484 GMP 5'-nucleotidase activity [PMID:29752939]
5'-deoxynucleotidase involved in deoxyribonucleoside monophosphate degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
EXISTENCE:direct assay:GO:0043541 UDP-N-acetylglucosamine transferase complex [PMID:19129246]
EXISTENCE:mutant phenotype:GO:0003975 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity [PMID:6096695]
EXISTENCE:mutant phenotype:GO:0006487 protein N-linked glycosylation [PMID:15794922]
EXISTENCE:mutant phenotype:GO:0009060 aerobic respiration [PMID:15794922]
UDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin; human homolog DPAGT1 can complement yeast ALG7 mutant
EXISTENCE:direct assay:GO:0004602 glutathione peroxidase activity [PMID:11445588|PMID:11875065]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21763276|PMID:14562095]
EXISTENCE:direct assay:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
EXISTENCE:mutant phenotype:GO:0004602 glutathione peroxidase activity [PMID:10480913]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:11445588]
EXISTENCE:mutant phenotype:GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity [PMID:11445588]
Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000182 rDNA binding [PMID:17259992]
EXISTENCE:direct assay:GO:0000976 transcription cis-regulatory region binding [PMID:23468649]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:12482963]
EXISTENCE:direct assay:GO:0003730 mRNA 3'-UTR binding [PMID:36284099]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922|PMID:36284099]
EXISTENCE:direct assay:GO:0006354 DNA-templated transcription elongation [PMID:15949446]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:12482963]
EXISTENCE:direct assay:GO:0030874 nucleolar chromatin [PMID:17259992]
EXISTENCE:direct assay:GO:0031491 nucleosome binding [PMID:12482963]
EXISTENCE:direct assay:GO:0036436 Isw1a complex [PMID:12482963]
EXISTENCE:direct assay:GO:0036437 Isw1b complex [PMID:12482963]
EXISTENCE:genetic interaction:GO:0001178 regulation of transcriptional start site selection at RNA polymerase II promoter [PMID:19948887]
EXISTENCE:genetic interaction:GO:0006363 termination of RNA polymerase I transcription [PMID:17259992]
EXISTENCE:genetic interaction:GO:0006369 termination of RNA polymerase II transcription [PMID:12504018]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:11238381]
EXISTENCE:mutant phenotype:GO:0006354 DNA-templated transcription elongation [PMID:15949446]
EXISTENCE:mutant phenotype:GO:0007062 sister chromatid cohesion [PMID:27185881]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:16468993]
EXISTENCE:mutant phenotype:GO:0046832 negative regulation of RNA export from nucleus [PMID:36284099]
EXISTENCE:mutant phenotype:GO:1902275 regulation of chromatin organization [PMID:22922743]
EXISTENCE:mutant phenotype:GO:1903895 negative regulation of IRE1-mediated unfolded protein response [PMID:36284099]
ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; binds to 3'UTR of HAC1 mRNA and limits its nuclear export attenuating the UPR
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:21880895]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:21880895]
EXISTENCE:direct assay:GO:0061685 diphthine methylesterase activity [PMID:24739148]
EXISTENCE:mutant phenotype:GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine [PMID:22188241]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:21880895]
EXISTENCE:mutant phenotype:GO:0061685 diphthine methylesterase activity [PMID:24739148]
Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins
snoRNA-binding rRNA-processing protein ENP1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9034325]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:12150911|PMID:15167894]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12150911|PMID:15167894]
EXISTENCE:direct assay:GO:0030515 snoRNA binding [PMID:12527778]
EXISTENCE:direct assay:GO:0030686 90S preribosome [PMID:12150911|PMID:15167894]
EXISTENCE:direct assay:GO:0030688 preribosome, small subunit precursor [PMID:16738661|PMID:15167894]
EXISTENCE:mutant phenotype:GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:15590835]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:12527778]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:27385342]
EXISTENCE:physical interaction:GO:0006364 rRNA processing [PMID:12527778]
Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant
EXISTENCE:curator inference:GO:0005737 cytoplasm [PMID:14190241]
EXISTENCE:direct assay:GO:0000107 imidazoleglycerol-phosphate synthase activity [PMID:10733892]
EXISTENCE:direct assay:GO:0004359 glutaminase activity [PMID:10733892]
EXISTENCE:mutant phenotype:GO:0000105 histidine biosynthetic process [PMID:14190241]
Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
3-deoxy-7-phosphoheptulonate synthase ARO4
EXISTENCE:direct assay:GO:0003849 3-deoxy-7-phosphoheptulonate synthase activity [PMID:2880280]
3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
EXISTENCE:mutant phenotype:GO:0051321 meiotic cell cycle [PMID:17179081]
EXISTENCE:physical interaction:GO:0051321 meiotic cell cycle [PMID:17179081]
hypothetical protein; associates with meiosis-specific protein Spo1p
mitochondrial 37S ribosomal protein MRPS5
EXISTENCE:direct assay:GO:0004170 dUTP diphosphatase activity [PMID:21548881]
EXISTENCE:direct assay:GO:0035863 dITP catabolic process [PMID:21548881]
EXISTENCE:direct assay:GO:0035870 dITP diphosphatase activity [PMID:21548881]
EXISTENCE:direct assay:GO:0046081 dUTP catabolic process [PMID:21548881]
EXISTENCE:mutant phenotype:GO:0004170 dUTP diphosphatase activity [PMID:21548881|PMID:8223452]
EXISTENCE:mutant phenotype:GO:0009213 pyrimidine deoxyribonucleoside triphosphate catabolic process [PMID:8223452]
EXISTENCE:mutant phenotype:GO:0046081 dUTP catabolic process [PMID:21548881]
Deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate; human homolog DUT allows growth of yeast haploid dut1 null mutant after sporulation of heterozygous diploid
EXISTENCE:direct assay:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8187178]
EXISTENCE:direct assay:GO:1990070 TRAPPI protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990071 TRAPPII protein complex [PMID:11239471]
EXISTENCE:direct assay:GO:1990072 TRAPPIII protein complex [PMID:20375281]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:9564032]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11239471]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:23465091|PMID:24329977]
Core component of transport protein particle (TRAPP) complexes I-III; TRAPPs are multimeric guanine nucleotide-exchange factors for GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation leads to defects in endocytic recycling, block in sporulation/meiosis; mutations in human homolog TRAPPC2 cause spondyloepiphyseal dysplasia tarda, TRAPPC2 can complement yeast null mutant
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:27663510]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:27663510]
hypothetical protein; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
Mitochondrial inner membrane protein; associates with respiratory chain supercomplexes III (ubiquinol-cytochrome c reductase) and IV (cytochrome c oxidase); identified by sequence comparison with hemiascomycetous yeast species
EXISTENCE:direct assay:GO:0004746 riboflavin synthase activity [PMID:7814407]
EXISTENCE:mutant phenotype:GO:0009231 riboflavin biosynthetic process [PMID:7814407]
Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854|PMID:9085845]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:23700311]
EXISTENCE:direct assay:GO:0030677 ribonuclease P complex [PMID:9085845]
EXISTENCE:direct assay:GO:0042134 rRNA primary transcript binding [PMID:17881380]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9085845]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9085845]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9085845]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9085845]
Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
EXISTENCE:direct assay:GO:0051568 histone H3-K4 methylation [PMID:11742990|PMID:11805083]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11742990|PMID:11805083]
Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
hypothetical protein; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:10526184]
EXISTENCE:direct assay:GO:0005546 phosphatidylinositol-4,5-bisphosphate binding [PMID:21215255|PMID:25620000]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:10931290]
EXISTENCE:direct assay:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:18845541]
EXISTENCE:direct assay:GO:0030479 actin cortical patch [PMID:10931290]
EXISTENCE:direct assay:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:18845541]
EXISTENCE:direct assay:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:18845541]
EXISTENCE:direct assay:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:18845541]
EXISTENCE:genetic interaction:GO:0005096 GTPase activator activity [PMID:10526184]
EXISTENCE:genetic interaction:GO:0006970 response to osmotic stress [PMID:10590461]
EXISTENCE:mutant phenotype:GO:0010314 phosphatidylinositol-5-phosphate binding [PMID:18845541]
EXISTENCE:mutant phenotype:GO:0010447 response to acidic pH [PMID:16087742]
EXISTENCE:mutant phenotype:GO:0031532 actin cytoskeleton reorganization [PMID:33508381]
EXISTENCE:mutant phenotype:GO:0032266 phosphatidylinositol-3-phosphate binding [PMID:18845541]
EXISTENCE:mutant phenotype:GO:0070273 phosphatidylinositol-4-phosphate binding [PMID:18845541]
EXISTENCE:mutant phenotype:GO:0080025 phosphatidylinositol-3,5-bisphosphate binding [PMID:18845541]
GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; involved in repolarization of actin cytoskeleton during adaptation to heat shock stress; phosphorylated by Pkc1p
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:20481588]
EXISTENCE:direct assay:GO:0018016 N-terminal peptidyl-proline dimethylation [PMID:20481588]
EXISTENCE:direct assay:GO:0071885 N-terminal protein N-methyltransferase activity [PMID:20481588]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:19055778]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:19055778]
EXISTENCE:mutant phenotype:GO:0018016 N-terminal peptidyl-proline dimethylation [PMID:20481588]
EXISTENCE:mutant phenotype:GO:0071885 N-terminal protein N-methyltransferase activity [PMID:20481588]
AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
EXISTENCE:direct assay:GO:0044284 mitochondrial crista junction [PMID:22009199]
EXISTENCE:direct assay:GO:0061617 MICOS complex [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0042407 cristae formation [PMID:21944719|PMID:21987634|PMID:22009199]
EXISTENCE:mutant phenotype:GO:0065003 protein-containing complex assembly [PMID:26968360]
Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic27p whose assembly and stability requires cardiolipin
EXISTENCE:genetic interaction:GO:0006730 one-carbon metabolic process [PMID:9398220]
EXISTENCE:mutant phenotype:GO:0004372 glycine hydroxymethyltransferase activity [PMID:1100397]
EXISTENCE:mutant phenotype:GO:0005739 mitochondrion [PMID:6271|PMID:9398220]
EXISTENCE:mutant phenotype:GO:0006730 one-carbon metabolic process [PMID:1100397]
Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
EXISTENCE:direct assay:GO:0019001 guanyl nucleotide binding [PMID:10394895]
EXISTENCE:mutant phenotype:GO:0007030 Golgi organization [PMID:10394895]
Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:14562095|PMID:11914276|PMID:26928762]
EXISTENCE:direct assay:GO:0006666 3-keto-sphinganine metabolic process [PMID:9804843]
EXISTENCE:direct assay:GO:0030148 sphingolipid biosynthetic process [PMID:9804843]
EXISTENCE:direct assay:GO:0047560 3-dehydrosphinganine reductase activity [PMID:9804843]
EXISTENCE:mutant phenotype:GO:0030148 sphingolipid biosynthetic process [PMID:9804843]
3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family; mutations in human homolog KDSR cause recessive progressive symmetric erythrokeratoderma
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15107529|PMID:14562095]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:16648468]
EXISTENCE:genetic interaction:GO:0000278 mitotic cell cycle [PMID:15107529]
EXISTENCE:genetic interaction:GO:0007117 budding cell bud growth [PMID:15107529]
EXISTENCE:genetic interaction:GO:0042273 ribosomal large subunit biogenesis [PMID:16648468]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:16651379]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:16651379|PMID:16648468]
Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
Protein with potential role in actin cytoskeleton organization; null mutant is slow-growing, sensitive to Congo Red, impaired in endocytosis, has increased lifespan; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
mitochondrial 54S ribosomal protein YmL37
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:2060626]
EXISTENCE:direct assay:GO:0003735 structural constituent of ribosome [PMID:2060626]
EXISTENCE:direct assay:GO:0005762 mitochondrial large ribosomal subunit [PMID:2060626]
Mitochondrial ribosomal protein of the large subunit
ARS224; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:24954416]
EXISTENCE:mutant phenotype:GO:0034553 mitochondrial respiratory chain complex II assembly [PMID:24954416]
EXISTENCE:mutant phenotype:GO:0044183 protein folding chaperone [PMID:24954416]
Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs
EXISTENCE:curator inference:GO:0038203 TORC2 signaling [PMID:15689497]
EXISTENCE:direct assay:GO:0031932 TORC2 complex [PMID:15689497]
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication
S-adenosylmethionine-dependent methyltransferase
EXISTENCE:direct assay:GO:0008757 S-adenosylmethionine-dependent methyltransferase activity [PMID:21858014]
EXISTENCE:direct assay:GO:0016279 protein-lysine N-methyltransferase activity [PMID:25086354]
EXISTENCE:mutant phenotype:GO:0016279 protein-lysine N-methyltransferase activity [PMID:22522802]
EXISTENCE:mutant phenotype:GO:0045905 positive regulation of translational termination [PMID:25231983]
S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:19446323]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:19446323]
EXISTENCE:direct assay:GO:0044183 protein folding chaperone [PMID:19412159]
EXISTENCE:genetic interaction:GO:0070682 proteasome regulatory particle assembly [PMID:19412159|PMID:19446322|PMID:19446323]
EXISTENCE:mutant phenotype:GO:0006298 mismatch repair [PMID:10681183]
EXISTENCE:mutant phenotype:GO:0070682 proteasome regulatory particle assembly [PMID:19217412|PMID:19446322|PMID:19446323]
Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:14755638]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:14755638]
EXISTENCE:mutant phenotype:GO:0030435 sporulation resulting in formation of a cellular spore [PMID:14755638]
UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11390356]
EXISTENCE:genetic interaction:GO:0048478 replication fork protection [PMID:18593882]
EXISTENCE:mutant phenotype:GO:0000077 DNA damage checkpoint signaling [PMID:10660074]
EXISTENCE:physical interaction:GO:0005634 nucleus [PMID:11390356]
Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
EXISTENCE:direct assay:GO:0000781 chromosome, telomeric region [PMID:9710643]
EXISTENCE:direct assay:GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding [PMID:30104203]
EXISTENCE:direct assay:GO:0003688 DNA replication origin binding [PMID:30104203]
EXISTENCE:direct assay:GO:0019237 centromeric DNA binding [PMID:30104203]
EXISTENCE:genetic interaction:GO:0006270 DNA replication initiation [PMID:24532715]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:1577274]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:24692507]
EXISTENCE:mutant phenotype:GO:0016233 telomere capping [PMID:20523746]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:1577274]
EXISTENCE:mutant phenotype:GO:0032297 negative regulation of DNA-templated DNA replication initiation [PMID:27820830]
EXISTENCE:mutant phenotype:GO:0070198 protein localization to chromosome, telomeric region [PMID:29529242]
EXISTENCE:mutant phenotype:GO:0097752 regulation of DNA stability [PMID:27820830]
EXISTENCE:mutant phenotype:GO:0101018 negative regulation of mitotic DNA replication initiation from late origin [PMID:27820830]
EXISTENCE:physical interaction:GO:0006270 DNA replication initiation [PMID:24532715]
EXISTENCE:physical interaction:GO:0070187 shelterin complex [PMID:23746845]
Protein involved in control of DNA replication; binds Rap1p C-terminus, telomeres, centromeres, replication origins; contributes to resection of DNA double strand breaks (DSBs); acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; in addition to controlling DNA replication initiation, plays ongoing role after initiation and controls events at blocked replication forks
ARS225; Autonomously Replicating Sequence; replication origin of very weak function
tyrosine/serine/threonine protein phosphatase PPS1
EXISTENCE:direct assay:GO:0008138 protein tyrosine/serine/threonine phosphatase activity [PMID:9083070]
EXISTENCE:mutant phenotype:GO:0033260 nuclear DNA replication [PMID:9083070]
Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
DNA polymerase epsilon noncatalytic subunit
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:14622139|PMID:16916794]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:14622139]
EXISTENCE:direct assay:GO:0006261 DNA-templated DNA replication [PMID:14622139|PMID:16369485]
EXISTENCE:direct assay:GO:0008622 epsilon DNA polymerase complex [PMID:12571237|PMID:2406268]
EXISTENCE:direct assay:GO:0030337 DNA polymerase processivity factor activity [PMID:16369485]
EXISTENCE:direct assay:GO:0042276 error-prone translesion synthesis [PMID:19776424]
EXISTENCE:genetic interaction:GO:0006272 leading strand elongation [PMID:21124948]
EXISTENCE:genetic interaction:GO:0008310 single-stranded DNA 3'-5' exodeoxyribonuclease activity [PMID:21124948]
EXISTENCE:genetic interaction:GO:0030337 DNA polymerase processivity factor activity [PMID:21124948]
EXISTENCE:mutant phenotype:GO:0006261 DNA-templated DNA replication [PMID:1923754]
EXISTENCE:mutant phenotype:GO:0006272 leading strand elongation [PMID:21124948]
Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000791 euchromatin [PMID:11983171]
EXISTENCE:direct assay:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:16246725]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:11983171]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:15149594|PMID:15643076]
EXISTENCE:direct assay:GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding [PMID:22796944]
EXISTENCE:direct assay:GO:2001209 positive regulation of transcription elongation by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006360 transcription by RNA polymerase I [PMID:20299458]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:genetic interaction:GO:0090262 regulation of transcription-coupled nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:10219085]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:15180994]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:16582434]
EXISTENCE:mutant phenotype:GO:0001015 snoRNA transcription by RNA polymerase II [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:11884586]
EXISTENCE:mutant phenotype:GO:0006360 transcription by RNA polymerase I [PMID:20299458]
EXISTENCE:mutant phenotype:GO:0006362 transcription elongation by RNA polymerase I [PMID:19164765]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:20299458]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11927560]
EXISTENCE:mutant phenotype:GO:0006383 transcription by RNA polymerase III [PMID:31501524]
EXISTENCE:mutant phenotype:GO:0031124 mRNA 3'-end processing [PMID:15149594]
EXISTENCE:mutant phenotype:GO:0031126 sno(s)RNA 3'-end processing [PMID:16246725]
EXISTENCE:mutant phenotype:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:14710186]
EXISTENCE:mutant phenotype:GO:0045910 negative regulation of DNA recombination [PMID:9891041]
EXISTENCE:mutant phenotype:GO:0070911 global genome nucleotide-excision repair [PMID:21737840]
EXISTENCE:mutant phenotype:GO:1901525 negative regulation of mitophagy [PMID:31525119]
EXISTENCE:mutant phenotype:GO:2001163 regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:15149594]
EXISTENCE:mutant phenotype:GO:2001165 positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [PMID:18469135]
EXISTENCE:physical interaction:GO:0000993 RNA polymerase II complex binding [PMID:16581788]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:11884586|PMID:11927560|PMID:9032243]
Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
SCF ubiquitin ligase complex subunit SAF1
EXISTENCE:direct assay:GO:0019005 SCF ubiquitin ligase complex [PMID:11283612|PMID:19882662]
EXISTENCE:mutant phenotype:GO:0004842 ubiquitin-protein transferase activity [PMID:17517885]
EXISTENCE:mutant phenotype:GO:0019005 SCF ubiquitin ligase complex [PMID:17517885]
EXISTENCE:mutant phenotype:GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [PMID:16677311|PMID:17517885]
EXISTENCE:physical interaction:GO:0019005 SCF ubiquitin ligase complex [PMID:17517885]
F-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:17179087]
EXISTENCE:direct assay:GO:0006751 glutathione catabolic process [PMID:22277648]
EXISTENCE:direct assay:GO:0036374 glutathione hydrolase activity [PMID:22277648]
EXISTENCE:direct assay:GO:0061672 glutathione hydrolase complex [PMID:22277648]
EXISTENCE:genetic interaction:GO:0008233 peptidase activity [PMID:17179087]
EXISTENCE:genetic interaction:GO:0008242 omega peptidase activity [PMID:17179087]
EXISTENCE:mutant phenotype:GO:0006751 glutathione catabolic process [PMID:22277648|PMID:17179087]
Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
mitochondrial 54S ribosomal protein YmL27
EXISTENCE:curator inference:GO:0032543 mitochondrial translation [PMID:12392552]
Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
EXISTENCE:direct assay:GO:0005048 signal sequence binding [PMID:12134063]
EXISTENCE:direct assay:GO:0071261 Ssh1 translocon complex [PMID:8612571]
EXISTENCE:genetic interaction:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:11702951|PMID:18347066]
EXISTENCE:mutant phenotype:GO:0006614 SRP-dependent cotranslational protein targeting to membrane [PMID:11702951]
Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
metallo-dependent hydrolase superfamily protein
Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
hypothetical protein; YBR285W is not an essential gene
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:8175799|PMID:26928762]
EXISTENCE:direct assay:GO:0004177 aminopeptidase activity [PMID:8175799]
EXISTENCE:direct assay:GO:0007039 protein catabolic process in the vacuole [PMID:8175799]
EXISTENCE:mutant phenotype:GO:0004177 aminopeptidase activity [PMID:8175800]
Vacuolar aminopeptidase Y; processed to mature form by Prb1p; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
hypothetical protein; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
EXISTENCE:direct assay:GO:0030123 AP-3 adaptor complex [PMID:9250663]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:17895371]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:9335339]
Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:2233708|PMID:22932476]
EXISTENCE:direct assay:GO:0016514 SWI/SNF complex [PMID:18644858|PMID:8016655|PMID:8127913|PMID:8159677]
EXISTENCE:genetic interaction:GO:0000436 carbon catabolite activation of transcription from RNA polymerase II promoter [PMID:14580348]
EXISTENCE:genetic interaction:GO:0006338 chromatin remodeling [PMID:1459453]
EXISTENCE:genetic interaction:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1901413]
EXISTENCE:mutant phenotype:GO:0000724 double-strand break repair via homologous recombination [PMID:16024655]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:1459453]
EXISTENCE:mutant phenotype:GO:0016514 SWI/SNF complex [PMID:8159677]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:1339306|PMID:3542227]
EXISTENCE:mutant phenotype:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:14580348]
EXISTENCE:physical interaction:GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding [PMID:11865042|PMID:14580348]
Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
EXISTENCE:direct assay:GO:0000324 fungal-type vacuole [PMID:23708375|PMID:26928762]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:14988731]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:9115231]
EXISTENCE:mutant phenotype:GO:0006511 ubiquitin-dependent protein catabolic process [PMID:16456538]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:14988731|PMID:9988727]
EXISTENCE:mutant phenotype:GO:0030001 metal ion transport [PMID:9115231]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:14988731]
Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
EXISTENCE:curator inference:GO:0005743 mitochondrial inner membrane [PMID:7876161]
EXISTENCE:direct assay:GO:0005371 tricarboxylate secondary active transmembrane transporter activity [PMID:7876161]
EXISTENCE:direct assay:GO:0006843 mitochondrial citrate transmembrane transport [PMID:7876161]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:7876161]
Mitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family
hypothetical protein; conserved across S. cerevisiae strains; YBR292C is not an essential gene
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:15572352]
EXISTENCE:direct assay:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015174 basic amino acid transmembrane transporter activity [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0015802 basic amino acid transport [PMID:15572352]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:15572352]
Permease of basic amino acids in the vacuolar membrane
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:7616962]
EXISTENCE:mutant phenotype:GO:0008272 sulfate transport [PMID:7616962]
EXISTENCE:mutant phenotype:GO:0015116 sulfate transmembrane transporter activity [PMID:7616962]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:7616962]
High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
ARS228; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0005839 proteasome core complex [PMID:27226596]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:27226596|PMID:17107946]
EXISTENCE:mutant phenotype:GO:0005507 copper ion binding [PMID:17107946]
EXISTENCE:mutant phenotype:GO:0006875 cellular metal ion homeostasis [PMID:10743563]
EXISTENCE:mutant phenotype:GO:0008551 P-type cadmium transporter activity [PMID:10743563|PMID:17107946]
EXISTENCE:mutant phenotype:GO:0015691 cadmium ion transport [PMID:17107946]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:17107946]
EXISTENCE:physical interaction:GO:0070628 proteasome binding [PMID:27226596]
Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
EXISTENCE:direct assay:GO:0015319 sodium:inorganic phosphate symporter activity [PMID:23216645]
EXISTENCE:direct assay:GO:0035435 phosphate ion transmembrane transport [PMID:23216645]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:9671031]
EXISTENCE:mutant phenotype:GO:0006817 phosphate ion transport [PMID:9671031]
EXISTENCE:mutant phenotype:GO:0015319 sodium:inorganic phosphate symporter activity [PMID:9671031]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:9671031]
Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
EXISTENCE:direct assay:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:29883473]
EXISTENCE:direct assay:GO:1990948 ubiquitin ligase inhibitor activity [PMID:29883473]
EXISTENCE:mutant phenotype:GO:1904667 negative regulation of ubiquitin protein ligase activity [PMID:29883473]
EXISTENCE:physical interaction:GO:0010997 anaphase-promoting complex binding [PMID:29883473]
Inhibitor of the Anaphase-Promoting Complex/Cyclosome (APC/C); binds to APC/C and inhibits the activity of both APC/C(Cdc20) and APC/C(Cdh1); inhibits the binding of Cdc20 and Cdh1 to APC/C; overexpression results in increased sensitivity to microtubule poison; localizes to peroxisomes; originally identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; functional homolog of human MAD2L1BP (p31comet)
MAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C
Maltose permease; high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C
EXISTENCE:direct assay:GO:0000025 maltose catabolic process [PMID:21873635]
EXISTENCE:direct assay:GO:0004575 sucrose alpha-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0005987 sucrose catabolic process [PMID:20471265]
EXISTENCE:direct assay:GO:0032450 maltose alpha-glucosidase activity [PMID:20471265]
EXISTENCE:direct assay:GO:0033934 glucan 1,4-alpha-maltotriohydrolase activity [PMID:20471265]
Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
EXISTENCE:direct assay:GO:0005768 endosome [PMID:25942624]
Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
TEL02R; Telomeric region on the right arm of Chromosome II; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats